MGMT cDNA ORF clone, Bos taurus(cattle)

The following MGMT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MGMT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa12259 XM_002698584.5
Latest version!
Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa12259 XM_001249417.6
Latest version!
Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa12259 XM_024986116.1
Latest version!
Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa12259 XM_005197119.3
Latest version!
Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa12259 XM_005225875.4
Latest version!
Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa231267 XM_024986115.1
Latest version!
Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OBa88794 XM_015460790.2
Latest version!
Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OBa88794 XM_015469656.1
Latest version!
Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
View Old Accession Versions >>
OBa12259 XM_002698584.4 Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa12259 XM_005225875.3 Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa88794 XM_015460790.1 Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa12259
    Clone ID Related Accession (Same CDS sequence) XM_002698584.4 , XM_002698584.5 , XM_001249417.6 , XM_005225875.3 , XM_024986116.1 , XM_005197119.3 , XM_005225875.4
    Accession Version XM_002698584.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product methylated-DNA--protein-cysteine methyltransferase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104587.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002698584.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATGAGA CCTGTGAAAT GAAATACAAA GTGGTGGACA GCCCTCTGGG GAAGTTGGAG 
    ATCTCTGGTT GTGAGCGGGG TCTGCATGGG ATAAAGCTGC ATGGCGGGAA GACCCCAGAC
    ACTGGGCCTG CGGAGGCCGC CGCTCCCGCT GAGCAGCTCG GAGGTTCCGG GGCAGTGCTG
    GAGCCCCTGC TGCAGTGCGC GGCCTGGCTG GACGCCTACT TCCGCGAGCC CGCAGTCCTT
    GAGGGGCTCC CCGTGCCTGC GCTGCACCAT CCCATCTTCC AGAAAGAGTC CTTCACCAGA
    CAGGTGTTAT GGAAGCTGCT GACGCTTGTG AAATTCGGAG AAACGGTTTC TTACCAGCAA
    TTAGCAGCCC TGGCGGGCAA CCCCAGAGCA GCGCGGGCCG TGGGAGGAGC CATGAGGAGC
    AACCCTATCC CCATCCTCAT CCCGTGCCAT CGAGTGGTCT GCAGCAGCGG GGCCATGGGC
    AACTACTCCG GAGGAGTGGG CGTGAAGGAG TGGCTGCTGG CCCACGAGGG CCGGCCCGCC
    GGGAAAGCAG CCCGTGGAGG GGGCTCCCGT GCCACCGGAT CCTGGCGCGG GGCCCTGGGT
    AGCACCGCCA GCTCCCAGCC CGCAGGCCGA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002698630.2
    CDS212..847
    Translation

    Target ORF information:

    RefSeq Version XM_002698584.4
    Organism Bos taurus(cattle)
    Definition Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002698584.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATGAGA CCTGTGAAAT GAAATACAAA GTGGTGGACA GCCCTCTGGG GAAGTTGGAG 
    ATCTCTGGTT GTGAGCGGGG TCTGCATGGG ATAAAGCTGC ATGGCGGGAA GACCCCAGAC
    ACTGGGCCTG CGGAGGCCGC CGCTCCCGCT GAGCAGCTCG GAGGTTCCGG GGCAGTGCTG
    GAGCCCCTGC TGCAGTGCGC GGCCTGGCTG GACGCCTACT TCCGCGAGCC CGCAGTCCTT
    GAGGGGCTCC CCGTGCCTGC GCTGCACCAT CCCATCTTCC AGAAAGAGTC CTTCACCAGA
    CAGGTGTTAT GGAAGCTGCT GACGCTTGTG AAATTCGGAG AAACGGTTTC TTACCAGCAA
    TTAGCAGCCC TGGCGGGCAA CCCCAGAGCA GCGCGGGCCG TGGGAGGAGC CATGAGGAGC
    AACCCTATCC CCATCCTCAT CCCGTGCCAT CGAGTGGTCT GCAGCAGCGG GGCCATGGGC
    AACTACTCCG GAGGAGTGGG CGTGAAGGAG TGGCTGCTGG CCCACGAGGG CCGGCCCGCC
    GGGAAAGCAG CCCGTGGAGG GGGCTCCCGT GCCACCGGAT CCTGGCGCGG GGCCCTGGGT
    AGCACCGCCA GCTCCCAGCC CGCAGGCCGA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12259
    Clone ID Related Accession (Same CDS sequence) XM_002698584.4 , XM_002698584.5 , XM_001249417.6 , XM_005225875.3 , XM_024986116.1 , XM_005197119.3 , XM_005225875.4
    Accession Version XM_002698584.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product methylated-DNA--protein-cysteine methyltransferase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002698584.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATGAGA CCTGTGAAAT GAAATACAAA GTGGTGGACA GCCCTCTGGG GAAGTTGGAG 
    ATCTCTGGTT GTGAGCGGGG TCTGCATGGG ATAAAGCTGC ATGGCGGGAA GACCCCAGAC
    ACTGGGCCTG CGGAGGCCGC CGCTCCCGCT GAGCAGCTCG GAGGTTCCGG GGCAGTGCTG
    GAGCCCCTGC TGCAGTGCGC GGCCTGGCTG GACGCCTACT TCCGCGAGCC CGCAGTCCTT
    GAGGGGCTCC CCGTGCCTGC GCTGCACCAT CCCATCTTCC AGAAAGAGTC CTTCACCAGA
    CAGGTGTTAT GGAAGCTGCT GACGCTTGTG AAATTCGGAG AAACGGTTTC TTACCAGCAA
    TTAGCAGCCC TGGCGGGCAA CCCCAGAGCA GCGCGGGCCG TGGGAGGAGC CATGAGGAGC
    AACCCTATCC CCATCCTCAT CCCGTGCCAT CGAGTGGTCT GCAGCAGCGG GGCCATGGGC
    AACTACTCCG GAGGAGTGGG CGTGAAGGAG TGGCTGCTGG CCCACGAGGG CCGGCCCGCC
    GGGAAAGCAG CCCGTGGAGG GGGCTCCCGT GCCACCGGAT CCTGGCGCGG GGCCCTGGGT
    AGCACCGCCA GCTCCCAGCC CGCAGGCCGA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002698630.2
    CDS216..851
    Translation

    Target ORF information:

    RefSeq Version XM_002698584.5
    Organism Bos taurus(cattle)
    Definition Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002698584.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATGAGA CCTGTGAAAT GAAATACAAA GTGGTGGACA GCCCTCTGGG GAAGTTGGAG 
    ATCTCTGGTT GTGAGCGGGG TCTGCATGGG ATAAAGCTGC ATGGCGGGAA GACCCCAGAC
    ACTGGGCCTG CGGAGGCCGC CGCTCCCGCT GAGCAGCTCG GAGGTTCCGG GGCAGTGCTG
    GAGCCCCTGC TGCAGTGCGC GGCCTGGCTG GACGCCTACT TCCGCGAGCC CGCAGTCCTT
    GAGGGGCTCC CCGTGCCTGC GCTGCACCAT CCCATCTTCC AGAAAGAGTC CTTCACCAGA
    CAGGTGTTAT GGAAGCTGCT GACGCTTGTG AAATTCGGAG AAACGGTTTC TTACCAGCAA
    TTAGCAGCCC TGGCGGGCAA CCCCAGAGCA GCGCGGGCCG TGGGAGGAGC CATGAGGAGC
    AACCCTATCC CCATCCTCAT CCCGTGCCAT CGAGTGGTCT GCAGCAGCGG GGCCATGGGC
    AACTACTCCG GAGGAGTGGG CGTGAAGGAG TGGCTGCTGG CCCACGAGGG CCGGCCCGCC
    GGGAAAGCAG CCCGTGGAGG GGGCTCCCGT GCCACCGGAT CCTGGCGCGG GGCCCTGGGT
    AGCACCGCCA GCTCCCAGCC CGCAGGCCGA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12259
    Clone ID Related Accession (Same CDS sequence) XM_002698584.4 , XM_002698584.5 , XM_001249417.6 , XM_005225875.3 , XM_024986116.1 , XM_005197119.3 , XM_005225875.4
    Accession Version XM_001249417.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product methylated-DNA--protein-cysteine methyltransferase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001249417.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATGAGA CCTGTGAAAT GAAATACAAA GTGGTGGACA GCCCTCTGGG GAAGTTGGAG 
    ATCTCTGGTT GTGAGCGGGG TCTGCATGGG ATAAAGCTGC ATGGCGGGAA GACCCCAGAC
    ACTGGGCCTG CGGAGGCCGC CGCTCCCGCT GAGCAGCTCG GAGGTTCCGG GGCAGTGCTG
    GAGCCCCTGC TGCAGTGCGC GGCCTGGCTG GACGCCTACT TCCGCGAGCC CGCAGTCCTT
    GAGGGGCTCC CCGTGCCTGC GCTGCACCAT CCCATCTTCC AGAAAGAGTC CTTCACCAGA
    CAGGTGTTAT GGAAGCTGCT GACGCTTGTG AAATTCGGAG AAACGGTTTC TTACCAGCAA
    TTAGCAGCCC TGGCGGGCAA CCCCAGAGCA GCGCGGGCCG TGGGAGGAGC CATGAGGAGC
    AACCCTATCC CCATCCTCAT CCCGTGCCAT CGAGTGGTCT GCAGCAGCGG GGCCATGGGC
    AACTACTCCG GAGGAGTGGG CGTGAAGGAG TGGCTGCTGG CCCACGAGGG CCGGCCCGCC
    GGGAAAGCAG CCCGTGGAGG GGGCTCCCGT GCCACCGGAT CCTGGCGCGG GGCCCTGGGT
    AGCACCGCCA GCTCCCAGCC CGCAGGCCGA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001249418.3
    CDS212..847
    Translation

    Target ORF information:

    RefSeq Version XM_001249417.6
    Organism Bos taurus(cattle)
    Definition Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001249417.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATGAGA CCTGTGAAAT GAAATACAAA GTGGTGGACA GCCCTCTGGG GAAGTTGGAG 
    ATCTCTGGTT GTGAGCGGGG TCTGCATGGG ATAAAGCTGC ATGGCGGGAA GACCCCAGAC
    ACTGGGCCTG CGGAGGCCGC CGCTCCCGCT GAGCAGCTCG GAGGTTCCGG GGCAGTGCTG
    GAGCCCCTGC TGCAGTGCGC GGCCTGGCTG GACGCCTACT TCCGCGAGCC CGCAGTCCTT
    GAGGGGCTCC CCGTGCCTGC GCTGCACCAT CCCATCTTCC AGAAAGAGTC CTTCACCAGA
    CAGGTGTTAT GGAAGCTGCT GACGCTTGTG AAATTCGGAG AAACGGTTTC TTACCAGCAA
    TTAGCAGCCC TGGCGGGCAA CCCCAGAGCA GCGCGGGCCG TGGGAGGAGC CATGAGGAGC
    AACCCTATCC CCATCCTCAT CCCGTGCCAT CGAGTGGTCT GCAGCAGCGG GGCCATGGGC
    AACTACTCCG GAGGAGTGGG CGTGAAGGAG TGGCTGCTGG CCCACGAGGG CCGGCCCGCC
    GGGAAAGCAG CCCGTGGAGG GGGCTCCCGT GCCACCGGAT CCTGGCGCGG GGCCCTGGGT
    AGCACCGCCA GCTCCCAGCC CGCAGGCCGA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12259
    Clone ID Related Accession (Same CDS sequence) XM_002698584.4 , XM_002698584.5 , XM_001249417.6 , XM_005225875.3 , XM_024986116.1 , XM_005197119.3 , XM_005225875.4
    Accession Version XM_005225875.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product methylated-DNA--protein-cysteine methyltransferase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104587.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225875.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATGAGA CCTGTGAAAT GAAATACAAA GTGGTGGACA GCCCTCTGGG GAAGTTGGAG 
    ATCTCTGGTT GTGAGCGGGG TCTGCATGGG ATAAAGCTGC ATGGCGGGAA GACCCCAGAC
    ACTGGGCCTG CGGAGGCCGC CGCTCCCGCT GAGCAGCTCG GAGGTTCCGG GGCAGTGCTG
    GAGCCCCTGC TGCAGTGCGC GGCCTGGCTG GACGCCTACT TCCGCGAGCC CGCAGTCCTT
    GAGGGGCTCC CCGTGCCTGC GCTGCACCAT CCCATCTTCC AGAAAGAGTC CTTCACCAGA
    CAGGTGTTAT GGAAGCTGCT GACGCTTGTG AAATTCGGAG AAACGGTTTC TTACCAGCAA
    TTAGCAGCCC TGGCGGGCAA CCCCAGAGCA GCGCGGGCCG TGGGAGGAGC CATGAGGAGC
    AACCCTATCC CCATCCTCAT CCCGTGCCAT CGAGTGGTCT GCAGCAGCGG GGCCATGGGC
    AACTACTCCG GAGGAGTGGG CGTGAAGGAG TGGCTGCTGG CCCACGAGGG CCGGCCCGCC
    GGGAAAGCAG CCCGTGGAGG GGGCTCCCGT GCCACCGGAT CCTGGCGCGG GGCCCTGGGT
    AGCACCGCCA GCTCCCAGCC CGCAGGCCGA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225932.1
    CDS102..737
    Translation

    Target ORF information:

    RefSeq Version XM_005225875.3
    Organism Bos taurus(cattle)
    Definition Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225875.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATGAGA CCTGTGAAAT GAAATACAAA GTGGTGGACA GCCCTCTGGG GAAGTTGGAG 
    ATCTCTGGTT GTGAGCGGGG TCTGCATGGG ATAAAGCTGC ATGGCGGGAA GACCCCAGAC
    ACTGGGCCTG CGGAGGCCGC CGCTCCCGCT GAGCAGCTCG GAGGTTCCGG GGCAGTGCTG
    GAGCCCCTGC TGCAGTGCGC GGCCTGGCTG GACGCCTACT TCCGCGAGCC CGCAGTCCTT
    GAGGGGCTCC CCGTGCCTGC GCTGCACCAT CCCATCTTCC AGAAAGAGTC CTTCACCAGA
    CAGGTGTTAT GGAAGCTGCT GACGCTTGTG AAATTCGGAG AAACGGTTTC TTACCAGCAA
    TTAGCAGCCC TGGCGGGCAA CCCCAGAGCA GCGCGGGCCG TGGGAGGAGC CATGAGGAGC
    AACCCTATCC CCATCCTCAT CCCGTGCCAT CGAGTGGTCT GCAGCAGCGG GGCCATGGGC
    AACTACTCCG GAGGAGTGGG CGTGAAGGAG TGGCTGCTGG CCCACGAGGG CCGGCCCGCC
    GGGAAAGCAG CCCGTGGAGG GGGCTCCCGT GCCACCGGAT CCTGGCGCGG GGCCCTGGGT
    AGCACCGCCA GCTCCCAGCC CGCAGGCCGA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12259
    Clone ID Related Accession (Same CDS sequence) XM_002698584.4 , XM_002698584.5 , XM_001249417.6 , XM_005225875.3 , XM_024986116.1 , XM_005197119.3 , XM_005225875.4
    Accession Version XM_024986116.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product methylated-DNA--protein-cysteine methyltransferase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATGAGA CCTGTGAAAT GAAATACAAA GTGGTGGACA GCCCTCTGGG GAAGTTGGAG 
    ATCTCTGGTT GTGAGCGGGG TCTGCATGGG ATAAAGCTGC ATGGCGGGAA GACCCCAGAC
    ACTGGGCCTG CGGAGGCCGC CGCTCCCGCT GAGCAGCTCG GAGGTTCCGG GGCAGTGCTG
    GAGCCCCTGC TGCAGTGCGC GGCCTGGCTG GACGCCTACT TCCGCGAGCC CGCAGTCCTT
    GAGGGGCTCC CCGTGCCTGC GCTGCACCAT CCCATCTTCC AGAAAGAGTC CTTCACCAGA
    CAGGTGTTAT GGAAGCTGCT GACGCTTGTG AAATTCGGAG AAACGGTTTC TTACCAGCAA
    TTAGCAGCCC TGGCGGGCAA CCCCAGAGCA GCGCGGGCCG TGGGAGGAGC CATGAGGAGC
    AACCCTATCC CCATCCTCAT CCCGTGCCAT CGAGTGGTCT GCAGCAGCGG GGCCATGGGC
    AACTACTCCG GAGGAGTGGG CGTGAAGGAG TGGCTGCTGG CCCACGAGGG CCGGCCCGCC
    GGGAAAGCAG CCCGTGGAGG GGGCTCCCGT GCCACCGGAT CCTGGCGCGG GGCCCTGGGT
    AGCACCGCCA GCTCCCAGCC CGCAGGCCGA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841884.1
    CDS509..1144
    Translation

    Target ORF information:

    RefSeq Version XM_024986116.1
    Organism Bos taurus(cattle)
    Definition Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986116.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATGAGA CCTGTGAAAT GAAATACAAA GTGGTGGACA GCCCTCTGGG GAAGTTGGAG 
    ATCTCTGGTT GTGAGCGGGG TCTGCATGGG ATAAAGCTGC ATGGCGGGAA GACCCCAGAC
    ACTGGGCCTG CGGAGGCCGC CGCTCCCGCT GAGCAGCTCG GAGGTTCCGG GGCAGTGCTG
    GAGCCCCTGC TGCAGTGCGC GGCCTGGCTG GACGCCTACT TCCGCGAGCC CGCAGTCCTT
    GAGGGGCTCC CCGTGCCTGC GCTGCACCAT CCCATCTTCC AGAAAGAGTC CTTCACCAGA
    CAGGTGTTAT GGAAGCTGCT GACGCTTGTG AAATTCGGAG AAACGGTTTC TTACCAGCAA
    TTAGCAGCCC TGGCGGGCAA CCCCAGAGCA GCGCGGGCCG TGGGAGGAGC CATGAGGAGC
    AACCCTATCC CCATCCTCAT CCCGTGCCAT CGAGTGGTCT GCAGCAGCGG GGCCATGGGC
    AACTACTCCG GAGGAGTGGG CGTGAAGGAG TGGCTGCTGG CCCACGAGGG CCGGCCCGCC
    GGGAAAGCAG CCCGTGGAGG GGGCTCCCGT GCCACCGGAT CCTGGCGCGG GGCCCTGGGT
    AGCACCGCCA GCTCCCAGCC CGCAGGCCGA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12259
    Clone ID Related Accession (Same CDS sequence) XM_002698584.4 , XM_002698584.5 , XM_001249417.6 , XM_005225875.3 , XM_024986116.1 , XM_005197119.3 , XM_005225875.4
    Accession Version XM_005197119.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product methylated-DNA--protein-cysteine methyltransferase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197119.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATGAGA CCTGTGAAAT GAAATACAAA GTGGTGGACA GCCCTCTGGG GAAGTTGGAG 
    ATCTCTGGTT GTGAGCGGGG TCTGCATGGG ATAAAGCTGC ATGGCGGGAA GACCCCAGAC
    ACTGGGCCTG CGGAGGCCGC CGCTCCCGCT GAGCAGCTCG GAGGTTCCGG GGCAGTGCTG
    GAGCCCCTGC TGCAGTGCGC GGCCTGGCTG GACGCCTACT TCCGCGAGCC CGCAGTCCTT
    GAGGGGCTCC CCGTGCCTGC GCTGCACCAT CCCATCTTCC AGAAAGAGTC CTTCACCAGA
    CAGGTGTTAT GGAAGCTGCT GACGCTTGTG AAATTCGGAG AAACGGTTTC TTACCAGCAA
    TTAGCAGCCC TGGCGGGCAA CCCCAGAGCA GCGCGGGCCG TGGGAGGAGC CATGAGGAGC
    AACCCTATCC CCATCCTCAT CCCGTGCCAT CGAGTGGTCT GCAGCAGCGG GGCCATGGGC
    AACTACTCCG GAGGAGTGGG CGTGAAGGAG TGGCTGCTGG CCCACGAGGG CCGGCCCGCC
    GGGAAAGCAG CCCGTGGAGG GGGCTCCCGT GCCACCGGAT CCTGGCGCGG GGCCCTGGGT
    AGCACCGCCA GCTCCCAGCC CGCAGGCCGA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005197176.1
    CDS102..737
    Translation

    Target ORF information:

    RefSeq Version XM_005197119.3
    Organism Bos taurus(cattle)
    Definition Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005197119.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATGAGA CCTGTGAAAT GAAATACAAA GTGGTGGACA GCCCTCTGGG GAAGTTGGAG 
    ATCTCTGGTT GTGAGCGGGG TCTGCATGGG ATAAAGCTGC ATGGCGGGAA GACCCCAGAC
    ACTGGGCCTG CGGAGGCCGC CGCTCCCGCT GAGCAGCTCG GAGGTTCCGG GGCAGTGCTG
    GAGCCCCTGC TGCAGTGCGC GGCCTGGCTG GACGCCTACT TCCGCGAGCC CGCAGTCCTT
    GAGGGGCTCC CCGTGCCTGC GCTGCACCAT CCCATCTTCC AGAAAGAGTC CTTCACCAGA
    CAGGTGTTAT GGAAGCTGCT GACGCTTGTG AAATTCGGAG AAACGGTTTC TTACCAGCAA
    TTAGCAGCCC TGGCGGGCAA CCCCAGAGCA GCGCGGGCCG TGGGAGGAGC CATGAGGAGC
    AACCCTATCC CCATCCTCAT CCCGTGCCAT CGAGTGGTCT GCAGCAGCGG GGCCATGGGC
    AACTACTCCG GAGGAGTGGG CGTGAAGGAG TGGCTGCTGG CCCACGAGGG CCGGCCCGCC
    GGGAAAGCAG CCCGTGGAGG GGGCTCCCGT GCCACCGGAT CCTGGCGCGG GGCCCTGGGT
    AGCACCGCCA GCTCCCAGCC CGCAGGCCGA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12259
    Clone ID Related Accession (Same CDS sequence) XM_002698584.4 , XM_002698584.5 , XM_001249417.6 , XM_005225875.3 , XM_024986116.1 , XM_005197119.3 , XM_005225875.4
    Accession Version XM_005225875.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product methylated-DNA--protein-cysteine methyltransferase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225875.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATGAGA CCTGTGAAAT GAAATACAAA GTGGTGGACA GCCCTCTGGG GAAGTTGGAG 
    ATCTCTGGTT GTGAGCGGGG TCTGCATGGG ATAAAGCTGC ATGGCGGGAA GACCCCAGAC
    ACTGGGCCTG CGGAGGCCGC CGCTCCCGCT GAGCAGCTCG GAGGTTCCGG GGCAGTGCTG
    GAGCCCCTGC TGCAGTGCGC GGCCTGGCTG GACGCCTACT TCCGCGAGCC CGCAGTCCTT
    GAGGGGCTCC CCGTGCCTGC GCTGCACCAT CCCATCTTCC AGAAAGAGTC CTTCACCAGA
    CAGGTGTTAT GGAAGCTGCT GACGCTTGTG AAATTCGGAG AAACGGTTTC TTACCAGCAA
    TTAGCAGCCC TGGCGGGCAA CCCCAGAGCA GCGCGGGCCG TGGGAGGAGC CATGAGGAGC
    AACCCTATCC CCATCCTCAT CCCGTGCCAT CGAGTGGTCT GCAGCAGCGG GGCCATGGGC
    AACTACTCCG GAGGAGTGGG CGTGAAGGAG TGGCTGCTGG CCCACGAGGG CCGGCCCGCC
    GGGAAAGCAG CCCGTGGAGG GGGCTCCCGT GCCACCGGAT CCTGGCGCGG GGCCCTGGGT
    AGCACCGCCA GCTCCCAGCC CGCAGGCCGA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225932.1
    CDS118..753
    Translation

    Target ORF information:

    RefSeq Version XM_005225875.4
    Organism Bos taurus(cattle)
    Definition Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225875.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGATGAGA CCTGTGAAAT GAAATACAAA GTGGTGGACA GCCCTCTGGG GAAGTTGGAG 
    ATCTCTGGTT GTGAGCGGGG TCTGCATGGG ATAAAGCTGC ATGGCGGGAA GACCCCAGAC
    ACTGGGCCTG CGGAGGCCGC CGCTCCCGCT GAGCAGCTCG GAGGTTCCGG GGCAGTGCTG
    GAGCCCCTGC TGCAGTGCGC GGCCTGGCTG GACGCCTACT TCCGCGAGCC CGCAGTCCTT
    GAGGGGCTCC CCGTGCCTGC GCTGCACCAT CCCATCTTCC AGAAAGAGTC CTTCACCAGA
    CAGGTGTTAT GGAAGCTGCT GACGCTTGTG AAATTCGGAG AAACGGTTTC TTACCAGCAA
    TTAGCAGCCC TGGCGGGCAA CCCCAGAGCA GCGCGGGCCG TGGGAGGAGC CATGAGGAGC
    AACCCTATCC CCATCCTCAT CCCGTGCCAT CGAGTGGTCT GCAGCAGCGG GGCCATGGGC
    AACTACTCCG GAGGAGTGGG CGTGAAGGAG TGGCTGCTGG CCCACGAGGG CCGGCCCGCC
    GGGAAAGCAG CCCGTGGAGG GGGCTCCCGT GCCACCGGAT CCTGGCGCGG GGCCCTGGGT
    AGCACCGCCA GCTCCCAGCC CGCAGGCCGA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231267
    Clone ID Related Accession (Same CDS sequence) XM_024986115.1
    Accession Version XM_024986115.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product methylated-DNA--protein-cysteine methyltransferase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTGGTGTT CCCGCGGAAC TCAGGTTCCC GTGAGAATTG GGGTTCTTAT GAGACCTGGG 
    GTTTCCTCGG GACTCGGGGT TCCCACGAGA CTCGGGGTTC CCGGCGCTGT GGAGTCGACC
    GCTCTCCCTG AGGTACTTGG GAGAATGGAT GAGACCTGTG AAATGAAATA CAAAGTGGTG
    GACAGCCCTC TGGGGAAGTT GGAGATCTCT GGTTGTGAGC GGGGTCTGCA TGGGATAAAG
    CTGCATGGCG GGAAGACCCC AGACACTGGG CCTGCGGAGG CCGCCGCTCC CGCTGAGCAG
    CTCGGAGGTT CCGGGGCAGT GCTGGAGCCC CTGCTGCAGT GCGCGGCCTG GCTGGACGCC
    TACTTCCGCG AGCCCGCAGT CCTTGAGGGG CTCCCCGTGC CTGCGCTGCA CCATCCCATC
    TTCCAGAAAG AGTCCTTCAC CAGACAGGTG TTATGGAAGC TGCTGACGCT TGTGAAATTC
    GGAGAAACGG TTTCTTACCA GCAATTAGCA GCCCTGGCGG GCAACCCCAG AGCAGCGCGG
    GCCGTGGGAG GAGCCATGAG GAGCAACCCT ATCCCCATCC TCATCCCGTG CCATCGAGTG
    GTCTGCAGCA GCGGGGCCAT GGGCAACTAC TCCGGAGGAG TGGGCGTGAA GGAGTGGCTG
    CTGGCCCACG AGGGCCGGCC CGCCGGGAAA GCAGCCCGTG GAGGGGGCTC CCGTGCCACC
    GGATCCTGGC GCGGGGCCCT GGGTAGCACC GCCAGCTCCC AGCCCGCAGG CCGAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841883.1
    CDS16..795
    Translation

    Target ORF information:

    RefSeq Version XM_024986115.1
    Organism Bos taurus(cattle)
    Definition Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986115.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTGGTGTT CCCGCGGAAC TCAGGTTCCC GTGAGAATTG GGGTTCTTAT GAGACCTGGG 
    GTTTCCTCGG GACTCGGGGT TCCCACGAGA CTCGGGGTTC CCGGCGCTGT GGAGTCGACC
    GCTCTCCCTG AGGTACTTGG GAGAATGGAT GAGACCTGTG AAATGAAATA CAAAGTGGTG
    GACAGCCCTC TGGGGAAGTT GGAGATCTCT GGTTGTGAGC GGGGTCTGCA TGGGATAAAG
    CTGCATGGCG GGAAGACCCC AGACACTGGG CCTGCGGAGG CCGCCGCTCC CGCTGAGCAG
    CTCGGAGGTT CCGGGGCAGT GCTGGAGCCC CTGCTGCAGT GCGCGGCCTG GCTGGACGCC
    TACTTCCGCG AGCCCGCAGT CCTTGAGGGG CTCCCCGTGC CTGCGCTGCA CCATCCCATC
    TTCCAGAAAG AGTCCTTCAC CAGACAGGTG TTATGGAAGC TGCTGACGCT TGTGAAATTC
    GGAGAAACGG TTTCTTACCA GCAATTAGCA GCCCTGGCGG GCAACCCCAG AGCAGCGCGG
    GCCGTGGGAG GAGCCATGAG GAGCAACCCT ATCCCCATCC TCATCCCGTG CCATCGAGTG
    GTCTGCAGCA GCGGGGCCAT GGGCAACTAC TCCGGAGGAG TGGGCGTGAA GGAGTGGCTG
    CTGGCCCACG AGGGCCGGCC CGCCGGGAAA GCAGCCCGTG GAGGGGGCTC CCGTGCCACC
    GGATCCTGGC GCGGGGCCCT GGGTAGCACC GCCAGCTCCC AGCCCGCAGG CCGAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88794
    Clone ID Related Accession (Same CDS sequence) XM_015469656.1 , XM_015460790.1 , XM_015460790.2
    Accession Version XM_015469656.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product methylated-DNA--protein-cysteine methyltransferase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACGCACC AACATTTCTC TCATCTTAAT GCCCCATTCA TGTGTGTGGA CTCTCCTTGG 
    CGTTTTGGTC TTTGTTTGCT TTGGAGCTAT TTAAAGCGAC ATTTATTGCC TGCGGAGGCC
    GCCGCTCCCG CTGAGCAGCT CGGAGGTTCC GGGGCAGTGC TGGAGCCCCT GCTGCAGTGC
    GCGGCCTGGC TGGACGCCTA CTTCCGCGAG CCCGCAGTCC TTGAGGGGCT CCCCGTGCCT
    GCGCTGCACC ATCCCATCTT CCAGAAAGAG TCCTTCACCA GACAGGTGTT ATGGAAGCTG
    CTGACGCTTG TGAAATTCGG AGAAACGGTT TCTTACCAGC AATTAGCAGC CCTGGCGGGC
    AACCCCAGAG CAGCGCGGGC CGTGGGAGGA GCCATGAGGA GCAACCCTAT CCCCATCCTC
    ATCCCGTGCC ATCGAGTGGT CTGCAGCAGC GGGGCCATGG GCAACTACTC CGGAGGAGTG
    GGCGTGAAGG AGTGGCTGCT GGCCCACGAG GGCCGGCCCG CCGGGAAAGC AGCCCGTGGA
    GGGGGCTCCC GTGCCACCGG ATCCTGGCGC GGGGCCCTGG GTAGCACCGC CAGCTCCCAG
    CCCGCAGGCC GAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325142.1
    CDS61..678
    Translation

    Target ORF information:

    RefSeq Version XM_015469656.1
    Organism Bos taurus(cattle)
    Definition Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469656.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACGCACC AACATTTCTC TCATCTTAAT GCCCCATTCA TGTGTGTGGA CTCTCCTTGG 
    CGTTTTGGTC TTTGTTTGCT TTGGAGCTAT TTAAAGCGAC ATTTATTGCC TGCGGAGGCC
    GCCGCTCCCG CTGAGCAGCT CGGAGGTTCC GGGGCAGTGC TGGAGCCCCT GCTGCAGTGC
    GCGGCCTGGC TGGACGCCTA CTTCCGCGAG CCCGCAGTCC TTGAGGGGCT CCCCGTGCCT
    GCGCTGCACC ATCCCATCTT CCAGAAAGAG TCCTTCACCA GACAGGTGTT ATGGAAGCTG
    CTGACGCTTG TGAAATTCGG AGAAACGGTT TCTTACCAGC AATTAGCAGC CCTGGCGGGC
    AACCCCAGAG CAGCGCGGGC CGTGGGAGGA GCCATGAGGA GCAACCCTAT CCCCATCCTC
    ATCCCGTGCC ATCGAGTGGT CTGCAGCAGC GGGGCCATGG GCAACTACTC CGGAGGAGTG
    GGCGTGAAGG AGTGGCTGCT GGCCCACGAG GGCCGGCCCG CCGGGAAAGC AGCCCGTGGA
    GGGGGCTCCC GTGCCACCGG ATCCTGGCGC GGGGCCCTGG GTAGCACCGC CAGCTCCCAG
    CCCGCAGGCC GAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88794
    Clone ID Related Accession (Same CDS sequence) XM_015469656.1 , XM_015460790.1 , XM_015460790.2
    Accession Version XM_015460790.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product methylated-DNA--protein-cysteine methyltransferase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104587.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACGCACC AACATTTCTC TCATCTTAAT GCCCCATTCA TGTGTGTGGA CTCTCCTTGG 
    CGTTTTGGTC TTTGTTTGCT TTGGAGCTAT TTAAAGCGAC ATTTATTGCC TGCGGAGGCC
    GCCGCTCCCG CTGAGCAGCT CGGAGGTTCC GGGGCAGTGC TGGAGCCCCT GCTGCAGTGC
    GCGGCCTGGC TGGACGCCTA CTTCCGCGAG CCCGCAGTCC TTGAGGGGCT CCCCGTGCCT
    GCGCTGCACC ATCCCATCTT CCAGAAAGAG TCCTTCACCA GACAGGTGTT ATGGAAGCTG
    CTGACGCTTG TGAAATTCGG AGAAACGGTT TCTTACCAGC AATTAGCAGC CCTGGCGGGC
    AACCCCAGAG CAGCGCGGGC CGTGGGAGGA GCCATGAGGA GCAACCCTAT CCCCATCCTC
    ATCCCGTGCC ATCGAGTGGT CTGCAGCAGC GGGGCCATGG GCAACTACTC CGGAGGAGTG
    GGCGTGAAGG AGTGGCTGCT GGCCCACGAG GGCCGGCCCG CCGGGAAAGC AGCCCGTGGA
    GGGGGCTCCC GTGCCACCGG ATCCTGGCGC GGGGCCCTGG GTAGCACCGC CAGCTCCCAG
    CCCGCAGGCC GAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316276.1
    CDS61..678
    Translation

    Target ORF information:

    RefSeq Version XM_015460790.1
    Organism Bos taurus(cattle)
    Definition Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460790.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACGCACC AACATTTCTC TCATCTTAAT GCCCCATTCA TGTGTGTGGA CTCTCCTTGG 
    CGTTTTGGTC TTTGTTTGCT TTGGAGCTAT TTAAAGCGAC ATTTATTGCC TGCGGAGGCC
    GCCGCTCCCG CTGAGCAGCT CGGAGGTTCC GGGGCAGTGC TGGAGCCCCT GCTGCAGTGC
    GCGGCCTGGC TGGACGCCTA CTTCCGCGAG CCCGCAGTCC TTGAGGGGCT CCCCGTGCCT
    GCGCTGCACC ATCCCATCTT CCAGAAAGAG TCCTTCACCA GACAGGTGTT ATGGAAGCTG
    CTGACGCTTG TGAAATTCGG AGAAACGGTT TCTTACCAGC AATTAGCAGC CCTGGCGGGC
    AACCCCAGAG CAGCGCGGGC CGTGGGAGGA GCCATGAGGA GCAACCCTAT CCCCATCCTC
    ATCCCGTGCC ATCGAGTGGT CTGCAGCAGC GGGGCCATGG GCAACTACTC CGGAGGAGTG
    GGCGTGAAGG AGTGGCTGCT GGCCCACGAG GGCCGGCCCG CCGGGAAAGC AGCCCGTGGA
    GGGGGCTCCC GTGCCACCGG ATCCTGGCGC GGGGCCCTGG GTAGCACCGC CAGCTCCCAG
    CCCGCAGGCC GAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88794
    Clone ID Related Accession (Same CDS sequence) XM_015469656.1 , XM_015460790.1 , XM_015460790.2
    Accession Version XM_015460790.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product methylated-DNA--protein-cysteine methyltransferase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460790.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACGCACC AACATTTCTC TCATCTTAAT GCCCCATTCA TGTGTGTGGA CTCTCCTTGG 
    CGTTTTGGTC TTTGTTTGCT TTGGAGCTAT TTAAAGCGAC ATTTATTGCC TGCGGAGGCC
    GCCGCTCCCG CTGAGCAGCT CGGAGGTTCC GGGGCAGTGC TGGAGCCCCT GCTGCAGTGC
    GCGGCCTGGC TGGACGCCTA CTTCCGCGAG CCCGCAGTCC TTGAGGGGCT CCCCGTGCCT
    GCGCTGCACC ATCCCATCTT CCAGAAAGAG TCCTTCACCA GACAGGTGTT ATGGAAGCTG
    CTGACGCTTG TGAAATTCGG AGAAACGGTT TCTTACCAGC AATTAGCAGC CCTGGCGGGC
    AACCCCAGAG CAGCGCGGGC CGTGGGAGGA GCCATGAGGA GCAACCCTAT CCCCATCCTC
    ATCCCGTGCC ATCGAGTGGT CTGCAGCAGC GGGGCCATGG GCAACTACTC CGGAGGAGTG
    GGCGTGAAGG AGTGGCTGCT GGCCCACGAG GGCCGGCCCG CCGGGAAAGC AGCCCGTGGA
    GGGGGCTCCC GTGCCACCGG ATCCTGGCGC GGGGCCCTGG GTAGCACCGC CAGCTCCCAG
    CCCGCAGGCC GAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316276.1
    CDS54..671
    Translation

    Target ORF information:

    RefSeq Version XM_015460790.2
    Organism Bos taurus(cattle)
    Definition Bos taurus O-6-methylguanine-DNA methyltransferase (MGMT), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460790.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACGCACC AACATTTCTC TCATCTTAAT GCCCCATTCA TGTGTGTGGA CTCTCCTTGG 
    CGTTTTGGTC TTTGTTTGCT TTGGAGCTAT TTAAAGCGAC ATTTATTGCC TGCGGAGGCC
    GCCGCTCCCG CTGAGCAGCT CGGAGGTTCC GGGGCAGTGC TGGAGCCCCT GCTGCAGTGC
    GCGGCCTGGC TGGACGCCTA CTTCCGCGAG CCCGCAGTCC TTGAGGGGCT CCCCGTGCCT
    GCGCTGCACC ATCCCATCTT CCAGAAAGAG TCCTTCACCA GACAGGTGTT ATGGAAGCTG
    CTGACGCTTG TGAAATTCGG AGAAACGGTT TCTTACCAGC AATTAGCAGC CCTGGCGGGC
    AACCCCAGAG CAGCGCGGGC CGTGGGAGGA GCCATGAGGA GCAACCCTAT CCCCATCCTC
    ATCCCGTGCC ATCGAGTGGT CTGCAGCAGC GGGGCCATGG GCAACTACTC CGGAGGAGTG
    GGCGTGAAGG AGTGGCTGCT GGCCCACGAG GGCCGGCCCG CCGGGAAAGC AGCCCGTGGA
    GGGGGCTCCC GTGCCACCGG ATCCTGGCGC GGGGCCCTGG GTAGCACCGC CAGCTCCCAG
    CCCGCAGGCC GAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.