PIWIL3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIWIL3
Entrez Gene ID	615479
Full Name	piwi like RNA-mediated gene silencing 3
General protein information	Preferred Names piwi like RNA-mediated gene silencing 3 Names piwi-like protein 3 piwi-like 3
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010823439.2	XP_010821741.1	piwi-like protein 3
NM_001319896.1	NP_001306825.1	piwi-like protein 3
XM_010814123.2	XP_010812425.1	piwi-like protein 3
XM_024977452.1	XP_024833220.1	piwi-like protein 3 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PIWIL3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIWIL3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa55767	XM_010823439.2 Latest version!	Bos taurus piwi-like RNA-mediated gene silencing 3 (PIWIL3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa55767	XM_010814123.2 Latest version!	Bos taurus piwi-like RNA-mediated gene silencing 3 (PIWIL3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa95431	NM_001319896.1 Latest version!	Bos taurus piwi like RNA-mediated gene silencing 3 (PIWIL3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa224395	XM_024977452.1 Latest version!	Bos taurus piwi like RNA-mediated gene silencing 3 (PIWIL3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIWIL3 ( XM_010823439.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821741.1 Bos taurus piwi-like RNA-mediated gene silencing 3 (PIWIL3), mRNA.

Price & Availability↑

CloneID	OBa55767
Clone ID Related Accession (Same CDS sequence)	XM_010823439.2 , XM_010814123.2
Accession Version	XM_010823439.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	piwi-like protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646373.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823439.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGGCA GGGCCAGAGT TCACGCCCGA GGCAGACGGC GGGATACACC ACCCTCGGAG 
CCCGCTCAGC CCGAGGCGGC GAGGGGACAC CCAATGCCTC CAGTGCCCAG GACAGACCCA 
CCACTGAGCT TCGCTGACTT AGTGAGACGC GGCACAGCGG CTCAGCAGCC TCGTCAAATC 
CAGCCTAGAC TCCAGTCATT GACAGGTGTT AGACACGTGG CTGAGGCTGG ACCACCAGCA 
GGAGCAGAAG CGACGTCTCA GCCTTCAGAA GAACTTAAAC CGGCATCTGA ATCACAGGAA 
TCCTTGCTGA GGAAGAAGAG GGATGTTGGT AGAGATTATC GAGACTTTGT GGTAAATACT 
CGGCAGAATT TGGTCCACGT TAGAGAGTCG ACAAAGGGCA CGCAAGGTAG CCCGGTAATG 
CTATTTTCGA ATCATGTCCG GCTCAAGTCT TGCTCTCAGA GGCATTTATA CAAGTACAAC 
GTCATCTACA CGCCAGACAT AGAGGATGGA CGCAAACGGG AAGCTTTACT CTCTGAACTT 
GAAAAGTTAC TGGGAAACCG TTGTATATAC GATGGAAACT CTTTGCTGTT ACCTCACTCG 
CTAGGGGAAA CGAAAAAGGA AGTGGTCAGC AAGTTGAAAA ATGAACCTGT GAAGATAACC 
TTCGAGCTCT CCAGAGAACT CCAGACCACT TCTCCAGACT GCCTCCGCTA TTACAACATT 
CTCTTTAGAA AAATGTTGGA GAAGATGGAT TTGAACCAAA TTGGTCGCAA CTATTACAAT 
AAAAATAAGA AGACTGAGTT CAACGAGTAT AGGTTGGAAA TATGGCCTGG CTATGTTACT 
TCTATTCTTC CATATGAAAC TGGTCTTACT CTCTGTGCTG ATGTGAGCCA CAAACTGCTC 
CGAATGGAAA CTGCTTATGA CTTAATATCA CACACTCGTG AAAAAGCCAG AGGAGAAGAT 
GCCAAAGAGA AAATTCTTAG AAAATTAGTT GGGTCATCTG TTCTTACAAA GTATAACAAC 
AGAACCTACA GAGTGGATGA TATTATTTGG GAGATGTCTC CCAGCTCTAC ATTTACCAAA 
TCAGATGGCA GTGAAATCTC CTTTGTAGAC TACTACAAGG AGCGGTATGG TACAGTGGTC 
ACTACCTTAA ATCAACCACT TTTGATCACC AAAGGCAAAT GGAAAAAGAG CCGACAGGAC 
ACACCTCATC AGCCTATCAT GCTGGTTCCT GAGCTGTGCC ACCTGACAGG CTTAACGGAT 
GACATGCGTA AAGACTACAG AATGATGAGA GACTTGTCCA CCCACACGAG GATGGATCCT 
GATAGAAGGC AGCATAAACT GCTCACATTT ATGGATGCTT TAAGAAAAAA TAATACTGTG 
CAGAAGGAGC TTCGAGACTG GAATTTGGAA CTCGAGGAGG GGTTTTTGTC CTTTTCTGGA 
AGAACTCTGC AAGATGTAAG AATTCACCAA GGAAGAAGGA TGTTTGACAA CCATAAAGCA 
GACTGGTCAA AAAACACAAG GGAGGCACCA TTACTTAGAG CAATGTCACT GGATCATTGG 
CTAATAGTCT ATACTAAGGG AAATTATGAA ACAGCCCTGA CCTTGCAGCA GAATCTACAG 
AGAGTGACAC CCAAAATGGG CATAACTGTG AGAAATGCAA AACTGCTTGA AGCAGCTGAT 
ACAGTCCAAT CTTACATAAG AACCTTAGAA AAACATGCTT CACAAAAAAC ACAGATGGTC 
CTTTGCCTGC TGCCCACTGA CAACAAGGAA ATTTACGATG GCATAAAAAG ATACCTGTGC 
ATCAATTACC CAATTCCAAG CCAGTGCGTG TTGAAACGGA CCTTGGATAG ACCTAAGACG 
CCAGTGACCA TCGCTACAAA AATTGCTCTG CAGATGAACT GCAAGCTGGG AGGTGCCCTC 
TGGAAGGTGG ACATAGGATT GCAGAACGCA ATGTTCATCG GTATCGATTG TTTCCACGAT 
ATTGAACGTC GACGGAAGTC AGTCGCGGGA TTTGTGGCAA GCATCGATCC AGACCTGACG 
CGGTGGTTCT CTCAGTGTAT CATCCAGGAG TCGGGGCAGG AGCTTGTCAA GGGGCTGACA 
ACCTGCTTGT ATACTGCCCT GAAGCTCTGG TCTGAACAAA ATTCATCTCC GCCACGTTCT 
ATCATTGTGT ATCGAGATGG AGTGGGAGAC GGACAGCTTC AAGCGCTCAT AGATCAAGAA 
GTAAAACAGA TGGAGTCCTA CTTAGAGAAC TCTTACAGAG GACAAAAGGT CAGACTAACT 
TTCATCGTGG TGAAGAAACG AATAAATACC AGATTTTTTA TTGAAAAGGA TGGAGGGAGA 
CTTAACAATC CATCTCCAGG AACAGTTATT GACCTAAAGG TGACTAGGAC AGAATGGTAT 
GATTTTTTCA TTGTGAGTCA GTCTGTGCGG GAAGGTACTG GGACTGTCAC TCCTACTCAC 
TATAACGTCA TCCACGACAC GCTGTACTTG AGCCCGGATG CGGTCCAGGG TCTCACTTAC 
AGACTCTGCC ACATGTACTA CAATTTTTCG GGTATCATCC GAGTTCCTGC TCCTTGCCAT 
TATGCCCATA AACTGGCTTA CTTGGTGGGC CAGAGTCTTC ATCAACAACC ACACGAATCT 
CTGTCAAAAT CTCTCTTTTA CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821741.1
CDS	288..2954
Translation	MTGRARVHARGRRRDTPPSEPAQPEAARGHPMPPVPRTDPPLSFADLVRRGTAAQQPRQIQPRLQSLTGVRHVAEAGPPAGAEATSQPSEELKPASESQESLLRKKRDVGRDYRDFVVNTRQNLVHVRESTKGTQGSPVMLFSNHVRLKSCSQRHLYKYNVIYTPDIEDGRKREALLSELEKLLGNRCIYDGNSLLLPHSLGETKKEVVSKLKNEPVKITFELSRELQTTSPDCLRYYNILFRKMLEKMDLNQIGRNYYNKNKKTEFNEYRLEIWPGYVTSILPYETGLTLCADVSHKLLRMETAYDLISHTREKARGEDAKEKILRKLVGSSVLTKYNNRTYRVDDIIWEMSPSSTFTKSDGSEISFVDYYKERYGTVVTTLNQPLLITKGKWKKSRQDTPHQPIMLVPELCHLTGLTDDMRKDYRMMRDLSTHTRMDPDRRQHKLLTFMDALRKNNTVQKELRDWNLELEEGFLSFSGRTLQDVRIHQGRRMFDNHKADWSKNTREAPLLRAMSLDHWLIVYTKGNYETALTLQQNLQRVTPKMGITVRNAKLLEAADTVQSYIRTLEKHASQKTQMVLCLLPTDNKEIYDGIKRYLCINYPIPSQCVLKRTLDRPKTPVTIATKIALQMNCKLGGALWKVDIGLQNAMFIGIDCFHDIERRRKSVAGFVASIDPDLTRWFSQCIIQESGQELVKGLTTCLYTALKLWSEQNSSPPRSIIVYRDGVGDGQLQALIDQEVKQMESYLENSYRGQKVRLTFIVVKKRINTRFFIEKDGGRLNNPSPGTVIDLKVTRTEWYDFFIVSQSVREGTGTVTPTHYNVIHDTLYLSPDAVQGLTYRLCHMYYNFSGIIRVPAPCHYAHKLAYLVGQSLHQQPHESLSKSLFYL

Target ORF information:

RefSeq Version	XM_010823439.2
Organism	Bos taurus(cattle)
Definition	Bos taurus piwi-like RNA-mediated gene silencing 3 (PIWIL3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823439.2

ORF Insert Sequence:

ATGACCGGCA GGGCCAGAGT TCACGCCCGA GGCAGACGGC GGGATACACC ACCCTCGGAG 
CCCGCTCAGC CCGAGGCGGC GAGGGGACAC CCAATGCCTC CAGTGCCCAG GACAGACCCA 
CCACTGAGCT TCGCTGACTT AGTGAGACGC GGCACAGCGG CTCAGCAGCC TCGTCAAATC 
CAGCCTAGAC TCCAGTCATT GACAGGTGTT AGACACGTGG CTGAGGCTGG ACCACCAGCA 
GGAGCAGAAG CGACGTCTCA GCCTTCAGAA GAACTTAAAC CGGCATCTGA ATCACAGGAA 
TCCTTGCTGA GGAAGAAGAG GGATGTTGGT AGAGATTATC GAGACTTTGT GGTAAATACT 
CGGCAGAATT TGGTCCACGT TAGAGAGTCG ACAAAGGGCA CGCAAGGTAG CCCGGTAATG 
CTATTTTCGA ATCATGTCCG GCTCAAGTCT TGCTCTCAGA GGCATTTATA CAAGTACAAC 
GTCATCTACA CGCCAGACAT AGAGGATGGA CGCAAACGGG AAGCTTTACT CTCTGAACTT 
GAAAAGTTAC TGGGAAACCG TTGTATATAC GATGGAAACT CTTTGCTGTT ACCTCACTCG 
CTAGGGGAAA CGAAAAAGGA AGTGGTCAGC AAGTTGAAAA ATGAACCTGT GAAGATAACC 
TTCGAGCTCT CCAGAGAACT CCAGACCACT TCTCCAGACT GCCTCCGCTA TTACAACATT 
CTCTTTAGAA AAATGTTGGA GAAGATGGAT TTGAACCAAA TTGGTCGCAA CTATTACAAT 
AAAAATAAGA AGACTGAGTT CAACGAGTAT AGGTTGGAAA TATGGCCTGG CTATGTTACT 
TCTATTCTTC CATATGAAAC TGGTCTTACT CTCTGTGCTG ATGTGAGCCA CAAACTGCTC 
CGAATGGAAA CTGCTTATGA CTTAATATCA CACACTCGTG AAAAAGCCAG AGGAGAAGAT 
GCCAAAGAGA AAATTCTTAG AAAATTAGTT GGGTCATCTG TTCTTACAAA GTATAACAAC 
AGAACCTACA GAGTGGATGA TATTATTTGG GAGATGTCTC CCAGCTCTAC ATTTACCAAA 
TCAGATGGCA GTGAAATCTC CTTTGTAGAC TACTACAAGG AGCGGTATGG TACAGTGGTC 
ACTACCTTAA ATCAACCACT TTTGATCACC AAAGGCAAAT GGAAAAAGAG CCGACAGGAC 
ACACCTCATC AGCCTATCAT GCTGGTTCCT GAGCTGTGCC ACCTGACAGG CTTAACGGAT 
GACATGCGTA AAGACTACAG AATGATGAGA GACTTGTCCA CCCACACGAG GATGGATCCT 
GATAGAAGGC AGCATAAACT GCTCACATTT ATGGATGCTT TAAGAAAAAA TAATACTGTG 
CAGAAGGAGC TTCGAGACTG GAATTTGGAA CTCGAGGAGG GGTTTTTGTC CTTTTCTGGA 
AGAACTCTGC AAGATGTAAG AATTCACCAA GGAAGAAGGA TGTTTGACAA CCATAAAGCA 
GACTGGTCAA AAAACACAAG GGAGGCACCA TTACTTAGAG CAATGTCACT GGATCATTGG 
CTAATAGTCT ATACTAAGGG AAATTATGAA ACAGCCCTGA CCTTGCAGCA GAATCTACAG 
AGAGTGACAC CCAAAATGGG CATAACTGTG AGAAATGCAA AACTGCTTGA AGCAGCTGAT 
ACAGTCCAAT CTTACATAAG AACCTTAGAA AAACATGCTT CACAAAAAAC ACAGATGGTC 
CTTTGCCTGC TGCCCACTGA CAACAAGGAA ATTTACGATG GCATAAAAAG ATACCTGTGC 
ATCAATTACC CAATTCCAAG CCAGTGCGTG TTGAAACGGA CCTTGGATAG ACCTAAGACG 
CCAGTGACCA TCGCTACAAA AATTGCTCTG CAGATGAACT GCAAGCTGGG AGGTGCCCTC 
TGGAAGGTGG ACATAGGATT GCAGAACGCA ATGTTCATCG GTATCGATTG TTTCCACGAT 
ATTGAACGTC GACGGAAGTC AGTCGCGGGA TTTGTGGCAA GCATCGATCC AGACCTGACG 
CGGTGGTTCT CTCAGTGTAT CATCCAGGAG TCGGGGCAGG AGCTTGTCAA GGGGCTGACA 
ACCTGCTTGT ATACTGCCCT GAAGCTCTGG TCTGAACAAA ATTCATCTCC GCCACGTTCT 
ATCATTGTGT ATCGAGATGG AGTGGGAGAC GGACAGCTTC AAGCGCTCAT AGATCAAGAA 
GTAAAACAGA TGGAGTCCTA CTTAGAGAAC TCTTACAGAG GACAAAAGGT CAGACTAACT 
TTCATCGTGG TGAAGAAACG AATAAATACC AGATTTTTTA TTGAAAAGGA TGGAGGGAGA 
CTTAACAATC CATCTCCAGG AACAGTTATT GACCTAAAGG TGACTAGGAC AGAATGGTAT 
GATTTTTTCA TTGTGAGTCA GTCTGTGCGG GAAGGTACTG GGACTGTCAC TCCTACTCAC 
TATAACGTCA TCCACGACAC GCTGTACTTG AGCCCGGATG CGGTCCAGGG TCTCACTTAC 
AGACTCTGCC ACATGTACTA CAATTTTTCG GGTATCATCC GAGTTCCTGC TCCTTGCCAT 
TATGCCCATA AACTGGCTTA CTTGGTGGGC CAGAGTCTTC ATCAACAACC ACACGAATCT 
CTGTCAAAAT CTCTCTTTTA CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIWIL3 ( XM_010814123.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812425.1 Bos taurus piwi-like RNA-mediated gene silencing 3 (PIWIL3), mRNA.

Price & Availability↑

CloneID	OBa55767
Clone ID Related Accession (Same CDS sequence)	XM_010823439.2 , XM_010814123.2
Accession Version	XM_010814123.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	piwi-like protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104458.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814123.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGGCA GGGCCAGAGT TCACGCCCGA GGCAGACGGC GGGATACACC ACCCTCGGAG 
CCCGCTCAGC CCGAGGCGGC GAGGGGACAC CCAATGCCTC CAGTGCCCAG GACAGACCCA 
CCACTGAGCT TCGCTGACTT AGTGAGACGC GGCACAGCGG CTCAGCAGCC TCGTCAAATC 
CAGCCTAGAC TCCAGTCATT GACAGGTGTT AGACACGTGG CTGAGGCTGG ACCACCAGCA 
GGAGCAGAAG CGACGTCTCA GCCTTCAGAA GAACTTAAAC CGGCATCTGA ATCACAGGAA 
TCCTTGCTGA GGAAGAAGAG GGATGTTGGT AGAGATTATC GAGACTTTGT GGTAAATACT 
CGGCAGAATT TGGTCCACGT TAGAGAGTCG ACAAAGGGCA CGCAAGGTAG CCCGGTAATG 
CTATTTTCGA ATCATGTCCG GCTCAAGTCT TGCTCTCAGA GGCATTTATA CAAGTACAAC 
GTCATCTACA CGCCAGACAT AGAGGATGGA CGCAAACGGG AAGCTTTACT CTCTGAACTT 
GAAAAGTTAC TGGGAAACCG TTGTATATAC GATGGAAACT CTTTGCTGTT ACCTCACTCG 
CTAGGGGAAA CGAAAAAGGA AGTGGTCAGC AAGTTGAAAA ATGAACCTGT GAAGATAACC 
TTCGAGCTCT CCAGAGAACT CCAGACCACT TCTCCAGACT GCCTCCGCTA TTACAACATT 
CTCTTTAGAA AAATGTTGGA GAAGATGGAT TTGAACCAAA TTGGTCGCAA CTATTACAAT 
AAAAATAAGA AGACTGAGTT CAACGAGTAT AGGTTGGAAA TATGGCCTGG CTATGTTACT 
TCTATTCTTC CATATGAAAC TGGTCTTACT CTCTGTGCTG ATGTGAGCCA CAAACTGCTC 
CGAATGGAAA CTGCTTATGA CTTAATATCA CACACTCGTG AAAAAGCCAG AGGAGAAGAT 
GCCAAAGAGA AAATTCTTAG AAAATTAGTT GGGTCATCTG TTCTTACAAA GTATAACAAC 
AGAACCTACA GAGTGGATGA TATTATTTGG GAGATGTCTC CCAGCTCTAC ATTTACCAAA 
TCAGATGGCA GTGAAATCTC CTTTGTAGAC TACTACAAGG AGCGGTATGG TACAGTGGTC 
ACTACCTTAA ATCAACCACT TTTGATCACC AAAGGCAAAT GGAAAAAGAG CCGACAGGAC 
ACACCTCATC AGCCTATCAT GCTGGTTCCT GAGCTGTGCC ACCTGACAGG CTTAACGGAT 
GACATGCGTA AAGACTACAG AATGATGAGA GACTTGTCCA CCCACACGAG GATGGATCCT 
GATAGAAGGC AGCATAAACT GCTCACATTT ATGGATGCTT TAAGAAAAAA TAATACTGTG 
CAGAAGGAGC TTCGAGACTG GAATTTGGAA CTCGAGGAGG GGTTTTTGTC CTTTTCTGGA 
AGAACTCTGC AAGATGTAAG AATTCACCAA GGAAGAAGGA TGTTTGACAA CCATAAAGCA 
GACTGGTCAA AAAACACAAG GGAGGCACCA TTACTTAGAG CAATGTCACT GGATCATTGG 
CTAATAGTCT ATACTAAGGG AAATTATGAA ACAGCCCTGA CCTTGCAGCA GAATCTACAG 
AGAGTGACAC CCAAAATGGG CATAACTGTG AGAAATGCAA AACTGCTTGA AGCAGCTGAT 
ACAGTCCAAT CTTACATAAG AACCTTAGAA AAACATGCTT CACAAAAAAC ACAGATGGTC 
CTTTGCCTGC TGCCCACTGA CAACAAGGAA ATTTACGATG GCATAAAAAG ATACCTGTGC 
ATCAATTACC CAATTCCAAG CCAGTGCGTG TTGAAACGGA CCTTGGATAG ACCTAAGACG 
CCAGTGACCA TCGCTACAAA AATTGCTCTG CAGATGAACT GCAAGCTGGG AGGTGCCCTC 
TGGAAGGTGG ACATAGGATT GCAGAACGCA ATGTTCATCG GTATCGATTG TTTCCACGAT 
ATTGAACGTC GACGGAAGTC AGTCGCGGGA TTTGTGGCAA GCATCGATCC AGACCTGACG 
CGGTGGTTCT CTCAGTGTAT CATCCAGGAG TCGGGGCAGG AGCTTGTCAA GGGGCTGACA 
ACCTGCTTGT ATACTGCCCT GAAGCTCTGG TCTGAACAAA ATTCATCTCC GCCACGTTCT 
ATCATTGTGT ATCGAGATGG AGTGGGAGAC GGACAGCTTC AAGCGCTCAT AGATCAAGAA 
GTAAAACAGA TGGAGTCCTA CTTAGAGAAC TCTTACAGAG GACAAAAGGT CAGACTAACT 
TTCATCGTGG TGAAGAAACG AATAAATACC AGATTTTTTA TTGAAAAGGA TGGAGGGAGA 
CTTAACAATC CATCTCCAGG AACAGTTATT GACCTAAAGG TGACTAGGAC AGAATGGTAT 
GATTTTTTCA TTGTGAGTCA GTCTGTGCGG GAAGGTACTG GGACTGTCAC TCCTACTCAC 
TATAACGTCA TCCACGACAC GCTGTACTTG AGCCCGGATG CGGTCCAGGG TCTCACTTAC 
AGACTCTGCC ACATGTACTA CAATTTTTCG GGTATCATCC GAGTTCCTGC TCCTTGCCAT 
TATGCCCATA AACTGGCTTA CTTGGTGGGC CAGAGTCTTC ATCAACAACC ACACGAATCT 
CTGTCAAAAT CTCTCTTTTA CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812425.1
CDS	288..2954
Translation	MTGRARVHARGRRRDTPPSEPAQPEAARGHPMPPVPRTDPPLSFADLVRRGTAAQQPRQIQPRLQSLTGVRHVAEAGPPAGAEATSQPSEELKPASESQESLLRKKRDVGRDYRDFVVNTRQNLVHVRESTKGTQGSPVMLFSNHVRLKSCSQRHLYKYNVIYTPDIEDGRKREALLSELEKLLGNRCIYDGNSLLLPHSLGETKKEVVSKLKNEPVKITFELSRELQTTSPDCLRYYNILFRKMLEKMDLNQIGRNYYNKNKKTEFNEYRLEIWPGYVTSILPYETGLTLCADVSHKLLRMETAYDLISHTREKARGEDAKEKILRKLVGSSVLTKYNNRTYRVDDIIWEMSPSSTFTKSDGSEISFVDYYKERYGTVVTTLNQPLLITKGKWKKSRQDTPHQPIMLVPELCHLTGLTDDMRKDYRMMRDLSTHTRMDPDRRQHKLLTFMDALRKNNTVQKELRDWNLELEEGFLSFSGRTLQDVRIHQGRRMFDNHKADWSKNTREAPLLRAMSLDHWLIVYTKGNYETALTLQQNLQRVTPKMGITVRNAKLLEAADTVQSYIRTLEKHASQKTQMVLCLLPTDNKEIYDGIKRYLCINYPIPSQCVLKRTLDRPKTPVTIATKIALQMNCKLGGALWKVDIGLQNAMFIGIDCFHDIERRRKSVAGFVASIDPDLTRWFSQCIIQESGQELVKGLTTCLYTALKLWSEQNSSPPRSIIVYRDGVGDGQLQALIDQEVKQMESYLENSYRGQKVRLTFIVVKKRINTRFFIEKDGGRLNNPSPGTVIDLKVTRTEWYDFFIVSQSVREGTGTVTPTHYNVIHDTLYLSPDAVQGLTYRLCHMYYNFSGIIRVPAPCHYAHKLAYLVGQSLHQQPHESLSKSLFYL

Target ORF information:

RefSeq Version	XM_010814123.2
Organism	Bos taurus(cattle)
Definition	Bos taurus piwi-like RNA-mediated gene silencing 3 (PIWIL3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814123.2

ORF Insert Sequence:

ATGACCGGCA GGGCCAGAGT TCACGCCCGA GGCAGACGGC GGGATACACC ACCCTCGGAG 
CCCGCTCAGC CCGAGGCGGC GAGGGGACAC CCAATGCCTC CAGTGCCCAG GACAGACCCA 
CCACTGAGCT TCGCTGACTT AGTGAGACGC GGCACAGCGG CTCAGCAGCC TCGTCAAATC 
CAGCCTAGAC TCCAGTCATT GACAGGTGTT AGACACGTGG CTGAGGCTGG ACCACCAGCA 
GGAGCAGAAG CGACGTCTCA GCCTTCAGAA GAACTTAAAC CGGCATCTGA ATCACAGGAA 
TCCTTGCTGA GGAAGAAGAG GGATGTTGGT AGAGATTATC GAGACTTTGT GGTAAATACT 
CGGCAGAATT TGGTCCACGT TAGAGAGTCG ACAAAGGGCA CGCAAGGTAG CCCGGTAATG 
CTATTTTCGA ATCATGTCCG GCTCAAGTCT TGCTCTCAGA GGCATTTATA CAAGTACAAC 
GTCATCTACA CGCCAGACAT AGAGGATGGA CGCAAACGGG AAGCTTTACT CTCTGAACTT 
GAAAAGTTAC TGGGAAACCG TTGTATATAC GATGGAAACT CTTTGCTGTT ACCTCACTCG 
CTAGGGGAAA CGAAAAAGGA AGTGGTCAGC AAGTTGAAAA ATGAACCTGT GAAGATAACC 
TTCGAGCTCT CCAGAGAACT CCAGACCACT TCTCCAGACT GCCTCCGCTA TTACAACATT 
CTCTTTAGAA AAATGTTGGA GAAGATGGAT TTGAACCAAA TTGGTCGCAA CTATTACAAT 
AAAAATAAGA AGACTGAGTT CAACGAGTAT AGGTTGGAAA TATGGCCTGG CTATGTTACT 
TCTATTCTTC CATATGAAAC TGGTCTTACT CTCTGTGCTG ATGTGAGCCA CAAACTGCTC 
CGAATGGAAA CTGCTTATGA CTTAATATCA CACACTCGTG AAAAAGCCAG AGGAGAAGAT 
GCCAAAGAGA AAATTCTTAG AAAATTAGTT GGGTCATCTG TTCTTACAAA GTATAACAAC 
AGAACCTACA GAGTGGATGA TATTATTTGG GAGATGTCTC CCAGCTCTAC ATTTACCAAA 
TCAGATGGCA GTGAAATCTC CTTTGTAGAC TACTACAAGG AGCGGTATGG TACAGTGGTC 
ACTACCTTAA ATCAACCACT TTTGATCACC AAAGGCAAAT GGAAAAAGAG CCGACAGGAC 
ACACCTCATC AGCCTATCAT GCTGGTTCCT GAGCTGTGCC ACCTGACAGG CTTAACGGAT 
GACATGCGTA AAGACTACAG AATGATGAGA GACTTGTCCA CCCACACGAG GATGGATCCT 
GATAGAAGGC AGCATAAACT GCTCACATTT ATGGATGCTT TAAGAAAAAA TAATACTGTG 
CAGAAGGAGC TTCGAGACTG GAATTTGGAA CTCGAGGAGG GGTTTTTGTC CTTTTCTGGA 
AGAACTCTGC AAGATGTAAG AATTCACCAA GGAAGAAGGA TGTTTGACAA CCATAAAGCA 
GACTGGTCAA AAAACACAAG GGAGGCACCA TTACTTAGAG CAATGTCACT GGATCATTGG 
CTAATAGTCT ATACTAAGGG AAATTATGAA ACAGCCCTGA CCTTGCAGCA GAATCTACAG 
AGAGTGACAC CCAAAATGGG CATAACTGTG AGAAATGCAA AACTGCTTGA AGCAGCTGAT 
ACAGTCCAAT CTTACATAAG AACCTTAGAA AAACATGCTT CACAAAAAAC ACAGATGGTC 
CTTTGCCTGC TGCCCACTGA CAACAAGGAA ATTTACGATG GCATAAAAAG ATACCTGTGC 
ATCAATTACC CAATTCCAAG CCAGTGCGTG TTGAAACGGA CCTTGGATAG ACCTAAGACG 
CCAGTGACCA TCGCTACAAA AATTGCTCTG CAGATGAACT GCAAGCTGGG AGGTGCCCTC 
TGGAAGGTGG ACATAGGATT GCAGAACGCA ATGTTCATCG GTATCGATTG TTTCCACGAT 
ATTGAACGTC GACGGAAGTC AGTCGCGGGA TTTGTGGCAA GCATCGATCC AGACCTGACG 
CGGTGGTTCT CTCAGTGTAT CATCCAGGAG TCGGGGCAGG AGCTTGTCAA GGGGCTGACA 
ACCTGCTTGT ATACTGCCCT GAAGCTCTGG TCTGAACAAA ATTCATCTCC GCCACGTTCT 
ATCATTGTGT ATCGAGATGG AGTGGGAGAC GGACAGCTTC AAGCGCTCAT AGATCAAGAA 
GTAAAACAGA TGGAGTCCTA CTTAGAGAAC TCTTACAGAG GACAAAAGGT CAGACTAACT 
TTCATCGTGG TGAAGAAACG AATAAATACC AGATTTTTTA TTGAAAAGGA TGGAGGGAGA 
CTTAACAATC CATCTCCAGG AACAGTTATT GACCTAAAGG TGACTAGGAC AGAATGGTAT 
GATTTTTTCA TTGTGAGTCA GTCTGTGCGG GAAGGTACTG GGACTGTCAC TCCTACTCAC 
TATAACGTCA TCCACGACAC GCTGTACTTG AGCCCGGATG CGGTCCAGGG TCTCACTTAC 
AGACTCTGCC ACATGTACTA CAATTTTTCG GGTATCATCC GAGTTCCTGC TCCTTGCCAT 
TATGCCCATA AACTGGCTTA CTTGGTGGGC CAGAGTCTTC ATCAACAACC ACACGAATCT 
CTGTCAAAAT CTCTCTTTTA CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIWIL3 ( NM_001319896.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001306825.1 Bos taurus piwi like RNA-mediated gene silencing 3 (PIWIL3), mRNA.

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CloneID	OBa95431
Clone ID Related Accession (Same CDS sequence)	NM_001319896.1
Accession Version	NM_001319896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	piwi-like protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KR911819.1. ##Evidence-Data-START## Transcript exon combination :: KR911819.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145491 [ECO:0000350] ##Evidence-Data-END##

ATGACCGGCA GGGCCAGAGT TCACGCCCGA GGCAGACGGC GGGATACACC ACCCTCGGAG 
CCCGCTCAGC CCGAGGCGGC GAGGGGACAC CCAATGCCTC CAGTGCCCAG GACAGACCCA 
CCACTGAGCT TCGCTGACTT AGTGAGACGC GGCACAGCGG CTCAGCAGCC TCGTCAAATC 
CAGCCTAGAC TCCAGTCATT GACAGGTGTT AGACACGTGG CTGAGGCTGG ACCACCAGCA 
GGAGCAGAAG CGACGTCTCA GCCTTCAGAA GAACTTAAAC CGGCATCTGA ATCACAGGAA 
TCCTTGCTGA GGAAGAAGAG GGATGTTGGT AGAGATTATC GAGACTTTGT GGTAAATACT 
CGGCAGAATT TGGTCCACGT TAGAGAGTCG ACAAAGGGCA CGCAAGGTAG CCCGGTAATG 
CTATTTTCGA ATCATGTCCG GCTCAAGTCT TGCTCTCAGA GGCATTTATA CAAGTACAAC 
GTCATCTACA CGCCAGACAT AGAGGATGGA CGCAAACGGG AAGCTTTACT CTCTGAACTT 
GAAAAGTTAC TGGGAAACCG TTGTATATAC GATGGAAACT CTTTGCTGTT ACCTCACTCG 
CTAGGGGAAA CGAAAAAGGA AGTGGTCAGC AAGTTGAAAA ATGAACCTGT GAAGATAACC 
TTCGAGCTCT CCAGAGAACT CCAGACCACT TCTCCAGACT GCCTCCGCTA TTACAACATT 
CTCTTTAGAA AAATGTTGGA GAAGATGGAT TTGAACCAAA TTGGTCGCAA CTATTACAAT 
AAAAATAAGA AGACTGAGTT CAACGAGTAT AGGTTGGAAA TATGGCCTGG CTATGTTACT 
TCTATTCTTC CATATGAAAC TGGTCTTACT CTCTGTGCTG ATGTGAGCCA CAAACTGCTC 
CGAATGGAAA CTGCTTATGA CTTAATATCA CACACTCGTG AAAAAGCCAG AGGAGAAGAT 
GCCAAAGAGA AAATTCTTAG AAAATTAGTT GGGTCATCTG TTCTTACAAA GTATAACAAC 
AGAACCTACA GAGTGGATGA TATTATTTGG GAGATGTCTC CCAGCTCTAC ATTTACCAAA 
TCAGATGGCA GTGAAATCTC CTTTGTAGAC TACTACAAGG AGCGGTATGG TACAGTGGTC 
ACTACCTTAA ATCAACCACT TTTGATCACC AAAGGCAAAT GGAAAAAGAG CCGACAGGAC 
ACACCTCATC AGCCTATCAT GCTGGTTCCT GAGCTGTGCC ACCTGACAGG CTTAACGGAT 
GACATGCGTA AAGACTACAG AATGATGAGA GACTTGTCCA CCCACACGAG GATGGATCCT 
GATAGAAGGC AGCATAAACT GCTCACATTT ATGGATGCTT TAAGAAAAAA TAATACTGTG 
CAGAAGGAGC TTCGAGACTG GAATTTGGAA CTCGAGGAGG GGTTTTTGTC CTTTTCTGGA 
AGAACTCTGC AAGATGTAAG AATTCACCAA GGAAGAAGGA TGGTAAGATT TGACAACCAT 
AAAGCAGACT GGTCAAAAAA CACAAGGGAG GCACCATTAC TTAGAGCAAT GTCACTGGAT 
CATTGGCTAA TAGTCTATAC TAAGGGAAAT TATGAAACAG CCCTGACCTT GCAGCAGAAT 
CTACAGAGAG TGACACCCAA AATGGGCATA ACTGTGAGAA ATGCAAAACT GCTTGAAGCA 
GCTGATACAG TCCAATCTTA CATAAGAACC TTAGAAAAAC ATGCTTCACA AAAAACACAG 
ATGGTCCTTT GCCTGCTGCC CACTGACAAC AAGGAAATTT ACGATGGCAT AAAAAGATAC 
CTGTGCATCA ATTACCCAAT TCCAAGCCAG TGCGTGTTGA AACGGACCTT GGATAGACCT 
AAGACGCCAG TGACCATCGC TACAAAAATT GCTCTGCAGA TGAACTGCAA GCTGGGAGGT 
GCCCTCTGGA AGGTGGACAT AGGATTGCAG AACGCAATGT TCATCGGTAT CGATTGTTTC 
CACGATATTG AACGTCGACG GAAGTCAGTC GCGGGATTTG TGGCAAGCAT CGATCCAGAC 
CTGACGCGGT GGTTCTCTCA GTGTATCATC CAGGAGTCGG GGCAGGAGCT TGTCAAGGGG 
CTGACAACCT GCTTGTATAC TGCCCTGAAG CTCTGGTCTG AACAAAATTC ATCTCCGCCA 
CGTTCTATCA TTGTGTATCG AGATGGAGTG GGAGACGGAC AGCTTCAAGC GCTCATAGAT 
CAAGAAGTAA AACAGATGGA GTCCTACTTA GAGAACTCTT ACAGAGGACA AAAGGTCAGA 
CTAACTTTCA TCGTGGTGAA GAAACGAATA AATACCAGAT TTTTTATTGA AAAGGATGGA 
GGGAGACTTA ACAATCCATC TCCAGGAACA GTTATTGACC TAAAGGTGAC TAGGACAGAA 
TGGTATGATT TTTTCATTGT GAGTCAGTCT GTGCGGGAAG GTACTGGGAC TGTCACTCCT 
ACTCACTATA ACGTCATCCA CGACACGCTG TACTTGAGCC CGGATGCGGT CCAGGGTCTC 
ACTTACAGAC TCTGCCACAT GTACTACAAT TTTTCGGGTA TCATCCGAGT TCCTGCTCCT 
TGCCATTATG CCCATAAACT GGCTTACTTG GTGGGCCAGA GTCTTCATCA ACAACCACAC 
GAATCTCTGT CAAAATCTCT CTTTTACCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001306825.1
CDS	297..2969
Translation	MTGRARVHARGRRRDTPPSEPAQPEAARGHPMPPVPRTDPPLSFADLVRRGTAAQQPRQIQPRLQSLTGVRHVAEAGPPAGAEATSQPSEELKPASESQESLLRKKRDVGRDYRDFVVNTRQNLVHVRESTKGTQGSPVMLFSNHVRLKSCSQRHLYKYNVIYTPDIEDGRKREALLSELEKLLGNRCIYDGNSLLLPHSLGETKKEVVSKLKNEPVKITFELSRELQTTSPDCLRYYNILFRKMLEKMDLNQIGRNYYNKNKKTEFNEYRLEIWPGYVTSILPYETGLTLCADVSHKLLRMETAYDLISHTREKARGEDAKEKILRKLVGSSVLTKYNNRTYRVDDIIWEMSPSSTFTKSDGSEISFVDYYKERYGTVVTTLNQPLLITKGKWKKSRQDTPHQPIMLVPELCHLTGLTDDMRKDYRMMRDLSTHTRMDPDRRQHKLLTFMDALRKNNTVQKELRDWNLELEEGFLSFSGRTLQDVRIHQGRRMVRFDNHKADWSKNTREAPLLRAMSLDHWLIVYTKGNYETALTLQQNLQRVTPKMGITVRNAKLLEAADTVQSYIRTLEKHASQKTQMVLCLLPTDNKEIYDGIKRYLCINYPIPSQCVLKRTLDRPKTPVTIATKIALQMNCKLGGALWKVDIGLQNAMFIGIDCFHDIERRRKSVAGFVASIDPDLTRWFSQCIIQESGQELVKGLTTCLYTALKLWSEQNSSPPRSIIVYRDGVGDGQLQALIDQEVKQMESYLENSYRGQKVRLTFIVVKKRINTRFFIEKDGGRLNNPSPGTVIDLKVTRTEWYDFFIVSQSVREGTGTVTPTHYNVIHDTLYLSPDAVQGLTYRLCHMYYNFSGIIRVPAPCHYAHKLAYLVGQSLHQQPHESLSKSLFYL

Target ORF information:

RefSeq Version	NM_001319896.1
Organism	Bos taurus(cattle)
Definition	Bos taurus piwi like RNA-mediated gene silencing 3 (PIWIL3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001319896.1

ORF Insert Sequence:

ATGACCGGCA GGGCCAGAGT TCACGCCCGA GGCAGACGGC GGGATACACC ACCCTCGGAG 
CCCGCTCAGC CCGAGGCGGC GAGGGGACAC CCAATGCCTC CAGTGCCCAG GACAGACCCA 
CCACTGAGCT TCGCTGACTT AGTGAGACGC GGCACAGCGG CTCAGCAGCC TCGTCAAATC 
CAGCCTAGAC TCCAGTCATT GACAGGTGTT AGACACGTGG CTGAGGCTGG ACCACCAGCA 
GGAGCAGAAG CGACGTCTCA GCCTTCAGAA GAACTTAAAC CGGCATCTGA ATCACAGGAA 
TCCTTGCTGA GGAAGAAGAG GGATGTTGGT AGAGATTATC GAGACTTTGT GGTAAATACT 
CGGCAGAATT TGGTCCACGT TAGAGAGTCG ACAAAGGGCA CGCAAGGTAG CCCGGTAATG 
CTATTTTCGA ATCATGTCCG GCTCAAGTCT TGCTCTCAGA GGCATTTATA CAAGTACAAC 
GTCATCTACA CGCCAGACAT AGAGGATGGA CGCAAACGGG AAGCTTTACT CTCTGAACTT 
GAAAAGTTAC TGGGAAACCG TTGTATATAC GATGGAAACT CTTTGCTGTT ACCTCACTCG 
CTAGGGGAAA CGAAAAAGGA AGTGGTCAGC AAGTTGAAAA ATGAACCTGT GAAGATAACC 
TTCGAGCTCT CCAGAGAACT CCAGACCACT TCTCCAGACT GCCTCCGCTA TTACAACATT 
CTCTTTAGAA AAATGTTGGA GAAGATGGAT TTGAACCAAA TTGGTCGCAA CTATTACAAT 
AAAAATAAGA AGACTGAGTT CAACGAGTAT AGGTTGGAAA TATGGCCTGG CTATGTTACT 
TCTATTCTTC CATATGAAAC TGGTCTTACT CTCTGTGCTG ATGTGAGCCA CAAACTGCTC 
CGAATGGAAA CTGCTTATGA CTTAATATCA CACACTCGTG AAAAAGCCAG AGGAGAAGAT 
GCCAAAGAGA AAATTCTTAG AAAATTAGTT GGGTCATCTG TTCTTACAAA GTATAACAAC 
AGAACCTACA GAGTGGATGA TATTATTTGG GAGATGTCTC CCAGCTCTAC ATTTACCAAA 
TCAGATGGCA GTGAAATCTC CTTTGTAGAC TACTACAAGG AGCGGTATGG TACAGTGGTC 
ACTACCTTAA ATCAACCACT TTTGATCACC AAAGGCAAAT GGAAAAAGAG CCGACAGGAC 
ACACCTCATC AGCCTATCAT GCTGGTTCCT GAGCTGTGCC ACCTGACAGG CTTAACGGAT 
GACATGCGTA AAGACTACAG AATGATGAGA GACTTGTCCA CCCACACGAG GATGGATCCT 
GATAGAAGGC AGCATAAACT GCTCACATTT ATGGATGCTT TAAGAAAAAA TAATACTGTG 
CAGAAGGAGC TTCGAGACTG GAATTTGGAA CTCGAGGAGG GGTTTTTGTC CTTTTCTGGA 
AGAACTCTGC AAGATGTAAG AATTCACCAA GGAAGAAGGA TGGTAAGATT TGACAACCAT 
AAAGCAGACT GGTCAAAAAA CACAAGGGAG GCACCATTAC TTAGAGCAAT GTCACTGGAT 
CATTGGCTAA TAGTCTATAC TAAGGGAAAT TATGAAACAG CCCTGACCTT GCAGCAGAAT 
CTACAGAGAG TGACACCCAA AATGGGCATA ACTGTGAGAA ATGCAAAACT GCTTGAAGCA 
GCTGATACAG TCCAATCTTA CATAAGAACC TTAGAAAAAC ATGCTTCACA AAAAACACAG 
ATGGTCCTTT GCCTGCTGCC CACTGACAAC AAGGAAATTT ACGATGGCAT AAAAAGATAC 
CTGTGCATCA ATTACCCAAT TCCAAGCCAG TGCGTGTTGA AACGGACCTT GGATAGACCT 
AAGACGCCAG TGACCATCGC TACAAAAATT GCTCTGCAGA TGAACTGCAA GCTGGGAGGT 
GCCCTCTGGA AGGTGGACAT AGGATTGCAG AACGCAATGT TCATCGGTAT CGATTGTTTC 
CACGATATTG AACGTCGACG GAAGTCAGTC GCGGGATTTG TGGCAAGCAT CGATCCAGAC 
CTGACGCGGT GGTTCTCTCA GTGTATCATC CAGGAGTCGG GGCAGGAGCT TGTCAAGGGG 
CTGACAACCT GCTTGTATAC TGCCCTGAAG CTCTGGTCTG AACAAAATTC ATCTCCGCCA 
CGTTCTATCA TTGTGTATCG AGATGGAGTG GGAGACGGAC AGCTTCAAGC GCTCATAGAT 
CAAGAAGTAA AACAGATGGA GTCCTACTTA GAGAACTCTT ACAGAGGACA AAAGGTCAGA 
CTAACTTTCA TCGTGGTGAA GAAACGAATA AATACCAGAT TTTTTATTGA AAAGGATGGA 
GGGAGACTTA ACAATCCATC TCCAGGAACA GTTATTGACC TAAAGGTGAC TAGGACAGAA 
TGGTATGATT TTTTCATTGT GAGTCAGTCT GTGCGGGAAG GTACTGGGAC TGTCACTCCT 
ACTCACTATA ACGTCATCCA CGACACGCTG TACTTGAGCC CGGATGCGGT CCAGGGTCTC 
ACTTACAGAC TCTGCCACAT GTACTACAAT TTTTCGGGTA TCATCCGAGT TCCTGCTCCT 
TGCCATTATG CCCATAAACT GGCTTACTTG GTGGGCCAGA GTCTTCATCA ACAACCACAC 
GAATCTCTGT CAAAATCTCT CTTTTACCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIWIL3 ( XM_024977452.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833220.1 Bos taurus piwi like RNA-mediated gene silencing 3 (PIWIL3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa224395
Clone ID Related Accession (Same CDS sequence)	XM_024977452.1
Accession Version	XM_024977452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	piwi-like protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGGCA GGGCCAGAGT TCACGCCCGA GGCAGACGGC GGGATACACC ACCCTCGGAG 
CCCGCTCAGC CCGAGGCGGC GAGGGGACAC CCAATGCCTC CAGTGCCCAG GACAGACCCA 
CCACTGAGCT TCGCTGACTT AGTGAGACGC GGCACAGCGG CTCAGCAGCC TCGTCAAATC 
CAGCCTAGAC TCCAGTCATT GACAGGTGTT AGACACGTGG CTGAGGCTGG ACCACCAGCA 
GGAGCAGAAG CGACGTCTCA GCCTTCAGAA GAACTTAAAC CGGCATCTGA ATCACAGGAA 
TCCTTGCTGA GGAAGAAGAG GGATGTTGGT AGAGATTATC GAGACTTTGT GGTAAATACT 
CGGCAGAATT TGGTCCACGT TAGAGAGTCG ACAAAGGGCA CGCAAGGTAG CCCAGTAATG 
CTATTTTCGA ATCATGTCCG GCTCAAGTCT TGCTCTCAGA GGCATTTATA CAAGTACAAC 
GTCATCTACA CGCCAGACAT AGAGGATGGA CGCAAACGGG AAGCTTTACT CTCTGAACTT 
GAAAAGTTAC TGGGAAACCG TTGTATATAC GATGGAAACT CTTTGCTGTT ACCTCACTCG 
CTAGGGGAAA CGAAAAAGGA AGTGGTCAGC AAGTTGAAAA ATGAACCTGT GAAGATAACC 
TTCGAGCTCT CCAGAGAACT CCAGACCACT TCTCCAGACT GCCTCCGCTA TTACAACATT 
CTCTTTAGAA AAATGTTGGA GAAGATGGAT TTGAACCAAA TTGGTCGCAA CTATTACAAT 
AAAAATAAGA AGACTGAGTT CAACGAGTAT AGGTTGGAAA TATGGCCTGG CTATGTTACT 
TCTATTCTTC CATATGAAAC TGGTCTTACT CTCTGTGCTG ATGTGAGCCA CAAACTGCTC 
CGAATGGAAA CTGCTTATGA CTTAATATCA CACACTCGTG AAAAAGCCAG AGGAGAAGAT 
GCCAAAGAGA AAATTCTTAG AAAATTAGTT GGGTCATCTG TTCTTACAAA GTATAACAAC 
AGAACCTACA GAGTGGATGA TATTATTTGG GAGATGTCTC CCAGCTCTAC ATTTACCAAA 
TCAGATGGCA GTGAAATCTC CTTTGTAGAC TACTACAAGG AGCGGTATGG TACAGTGGTC 
ACTACCTTAA ATCAACCACT TTTGATCACC AAAGGCAAAT GGAAAAAGAG CCGACAGGAC 
ACACCTCATC AGCCTATCAT GCTGGTTCCT GAGCTGTGCC ACCTGACAGG CTTAACGGAT 
GACATGCGTA AAGACTACAG AATGATGAGA GACTTGTCCA CCCACACGAG GATGGATCCT 
GATAGAAGGC AGCATAAACT GCTCACATTT ATGGATGCTT TAAGAAAAAA TAATACTGTG 
CAGAAGGAGC TTCGAGACTG GAATTTGGAA CTCGAGGAGG GGTTTTTGTC CTTTTCTGGA 
AGAACTCTGC AAGATGTAAG AATTCACCAA GGAAGAAGGA TGTTTGACAA CCATAAAGCA 
GACTGGTCAA AAAACACAAG GGAGGCACCA TTACTTAGAG CAATGTCACT GGATCATTGG 
CTAATAGTCT ATACTAAGGG AAATTATGAA ACAGCCCTGA CCTTGCAGCA GAATCTACAG 
AGAGTGACAC CCAAAATGGG CATAACTGTG AGAAATGCAA AACTGCTTGA AGCAGCTGAT 
ACAGTCCAAT CTTACATAAG AACCTTAGAA AAACATGCTT CACAAAAAAC ACAGATGGTC 
CTTTGCCTGC TGCCCACTGA CAACAAGGAA ATTTACGATG GCATAAAAAG ATACCTGTGC 
ATCAATTACC CAATTCCAAG CCAGTGCGTG TTGAAACGGA CCTTGGATAG ACCTAAGACG 
CCAGTGACCA TCGCTACAAA AATTGCTCTG CAGATGAACT GCAAGCTGGG AGGTGCCCTC 
TGGAAGGTGG ACATAGGATT GCAGAACGCA ATGTTCATCG GTATCGATTG TTTCCACGAT 
ATTGAACGTC GACGGAAGTC AGTCGCGGGA TTTGTGGCAA GCATCGATCC AGACCTGACG 
CGGTGGTTCT CTCAGTGTAT CATCCAGGAG TCGGGGCAGG AGCTTGTCAA GGGGCTGACA 
ACCTGCTTGT ATACTGCCCT GAAGCTCTGG TCTGAACAAA ATTCATCTCC GCCACGTTCT 
ATCATTGTGT ATCGAGATGG AGTGGGAGAC GGACAGCTTC AAGCGCTCAT AGATCAAGAA 
GTAAAACAGA TGGAGTCCTA CTTAGAGAAC TCTTACAGAG GACAAAAGGT CAGACTAACT 
TTCATCGTGG TGAAGAAACG AATAAATACC AGATTTTTTA TTGAAAAGGA TGGAGGGAGA 
CTTAACAATC CATCTCCAGG AACAGTTATT GACCTAAAGG TGACTAGGAC AGAATGGTAT 
GATTTTTTCA TTGTGAGTCA GTCTGTGCGG GAAGGTACTG GGACTGTCAC TCCTACTCAC 
TATAACGTCA TCCACGACAC GCTGTACTTG AGCCCGGATG CGGTCCAGGG TCTCACTTAC 
AGACTCTGCC ACATGTACTA CAATTTTTCG GGTATCATCC GAGTTCCTGC TCCTTGCCAT 
TATGCCCATA AACTGGCTTA CTTGGTGGGC CAGAGTCTTC ATCAACAACC ACACGAATCT 
CTGTCAAAAT CTCTCTTTTA CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833220.1
CDS	132..2798
Translation	MTGRARVHARGRRRDTPPSEPAQPEAARGHPMPPVPRTDPPLSFADLVRRGTAAQQPRQIQPRLQSLTGVRHVAEAGPPAGAEATSQPSEELKPASESQESLLRKKRDVGRDYRDFVVNTRQNLVHVRESTKGTQGSPVMLFSNHVRLKSCSQRHLYKYNVIYTPDIEDGRKREALLSELEKLLGNRCIYDGNSLLLPHSLGETKKEVVSKLKNEPVKITFELSRELQTTSPDCLRYYNILFRKMLEKMDLNQIGRNYYNKNKKTEFNEYRLEIWPGYVTSILPYETGLTLCADVSHKLLRMETAYDLISHTREKARGEDAKEKILRKLVGSSVLTKYNNRTYRVDDIIWEMSPSSTFTKSDGSEISFVDYYKERYGTVVTTLNQPLLITKGKWKKSRQDTPHQPIMLVPELCHLTGLTDDMRKDYRMMRDLSTHTRMDPDRRQHKLLTFMDALRKNNTVQKELRDWNLELEEGFLSFSGRTLQDVRIHQGRRMFDNHKADWSKNTREAPLLRAMSLDHWLIVYTKGNYETALTLQQNLQRVTPKMGITVRNAKLLEAADTVQSYIRTLEKHASQKTQMVLCLLPTDNKEIYDGIKRYLCINYPIPSQCVLKRTLDRPKTPVTIATKIALQMNCKLGGALWKVDIGLQNAMFIGIDCFHDIERRRKSVAGFVASIDPDLTRWFSQCIIQESGQELVKGLTTCLYTALKLWSEQNSSPPRSIIVYRDGVGDGQLQALIDQEVKQMESYLENSYRGQKVRLTFIVVKKRINTRFFIEKDGGRLNNPSPGTVIDLKVTRTEWYDFFIVSQSVREGTGTVTPTHYNVIHDTLYLSPDAVQGLTYRLCHMYYNFSGIIRVPAPCHYAHKLAYLVGQSLHQQPHESLSKSLFYL

Target ORF information:

RefSeq Version	XM_024977452.1
Organism	Bos taurus(cattle)
Definition	Bos taurus piwi like RNA-mediated gene silencing 3 (PIWIL3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977452.1

ORF Insert Sequence:

ATGACCGGCA GGGCCAGAGT TCACGCCCGA GGCAGACGGC GGGATACACC ACCCTCGGAG 
CCCGCTCAGC CCGAGGCGGC GAGGGGACAC CCAATGCCTC CAGTGCCCAG GACAGACCCA 
CCACTGAGCT TCGCTGACTT AGTGAGACGC GGCACAGCGG CTCAGCAGCC TCGTCAAATC 
CAGCCTAGAC TCCAGTCATT GACAGGTGTT AGACACGTGG CTGAGGCTGG ACCACCAGCA 
GGAGCAGAAG CGACGTCTCA GCCTTCAGAA GAACTTAAAC CGGCATCTGA ATCACAGGAA 
TCCTTGCTGA GGAAGAAGAG GGATGTTGGT AGAGATTATC GAGACTTTGT GGTAAATACT 
CGGCAGAATT TGGTCCACGT TAGAGAGTCG ACAAAGGGCA CGCAAGGTAG CCCAGTAATG 
CTATTTTCGA ATCATGTCCG GCTCAAGTCT TGCTCTCAGA GGCATTTATA CAAGTACAAC 
GTCATCTACA CGCCAGACAT AGAGGATGGA CGCAAACGGG AAGCTTTACT CTCTGAACTT 
GAAAAGTTAC TGGGAAACCG TTGTATATAC GATGGAAACT CTTTGCTGTT ACCTCACTCG 
CTAGGGGAAA CGAAAAAGGA AGTGGTCAGC AAGTTGAAAA ATGAACCTGT GAAGATAACC 
TTCGAGCTCT CCAGAGAACT CCAGACCACT TCTCCAGACT GCCTCCGCTA TTACAACATT 
CTCTTTAGAA AAATGTTGGA GAAGATGGAT TTGAACCAAA TTGGTCGCAA CTATTACAAT 
AAAAATAAGA AGACTGAGTT CAACGAGTAT AGGTTGGAAA TATGGCCTGG CTATGTTACT 
TCTATTCTTC CATATGAAAC TGGTCTTACT CTCTGTGCTG ATGTGAGCCA CAAACTGCTC 
CGAATGGAAA CTGCTTATGA CTTAATATCA CACACTCGTG AAAAAGCCAG AGGAGAAGAT 
GCCAAAGAGA AAATTCTTAG AAAATTAGTT GGGTCATCTG TTCTTACAAA GTATAACAAC 
AGAACCTACA GAGTGGATGA TATTATTTGG GAGATGTCTC CCAGCTCTAC ATTTACCAAA 
TCAGATGGCA GTGAAATCTC CTTTGTAGAC TACTACAAGG AGCGGTATGG TACAGTGGTC 
ACTACCTTAA ATCAACCACT TTTGATCACC AAAGGCAAAT GGAAAAAGAG CCGACAGGAC 
ACACCTCATC AGCCTATCAT GCTGGTTCCT GAGCTGTGCC ACCTGACAGG CTTAACGGAT 
GACATGCGTA AAGACTACAG AATGATGAGA GACTTGTCCA CCCACACGAG GATGGATCCT 
GATAGAAGGC AGCATAAACT GCTCACATTT ATGGATGCTT TAAGAAAAAA TAATACTGTG 
CAGAAGGAGC TTCGAGACTG GAATTTGGAA CTCGAGGAGG GGTTTTTGTC CTTTTCTGGA 
AGAACTCTGC AAGATGTAAG AATTCACCAA GGAAGAAGGA TGTTTGACAA CCATAAAGCA 
GACTGGTCAA AAAACACAAG GGAGGCACCA TTACTTAGAG CAATGTCACT GGATCATTGG 
CTAATAGTCT ATACTAAGGG AAATTATGAA ACAGCCCTGA CCTTGCAGCA GAATCTACAG 
AGAGTGACAC CCAAAATGGG CATAACTGTG AGAAATGCAA AACTGCTTGA AGCAGCTGAT 
ACAGTCCAAT CTTACATAAG AACCTTAGAA AAACATGCTT CACAAAAAAC ACAGATGGTC 
CTTTGCCTGC TGCCCACTGA CAACAAGGAA ATTTACGATG GCATAAAAAG ATACCTGTGC 
ATCAATTACC CAATTCCAAG CCAGTGCGTG TTGAAACGGA CCTTGGATAG ACCTAAGACG 
CCAGTGACCA TCGCTACAAA AATTGCTCTG CAGATGAACT GCAAGCTGGG AGGTGCCCTC 
TGGAAGGTGG ACATAGGATT GCAGAACGCA ATGTTCATCG GTATCGATTG TTTCCACGAT 
ATTGAACGTC GACGGAAGTC AGTCGCGGGA TTTGTGGCAA GCATCGATCC AGACCTGACG 
CGGTGGTTCT CTCAGTGTAT CATCCAGGAG TCGGGGCAGG AGCTTGTCAA GGGGCTGACA 
ACCTGCTTGT ATACTGCCCT GAAGCTCTGG TCTGAACAAA ATTCATCTCC GCCACGTTCT 
ATCATTGTGT ATCGAGATGG AGTGGGAGAC GGACAGCTTC AAGCGCTCAT AGATCAAGAA 
GTAAAACAGA TGGAGTCCTA CTTAGAGAAC TCTTACAGAG GACAAAAGGT CAGACTAACT 
TTCATCGTGG TGAAGAAACG AATAAATACC AGATTTTTTA TTGAAAAGGA TGGAGGGAGA 
CTTAACAATC CATCTCCAGG AACAGTTATT GACCTAAAGG TGACTAGGAC AGAATGGTAT 
GATTTTTTCA TTGTGAGTCA GTCTGTGCGG GAAGGTACTG GGACTGTCAC TCCTACTCAC 
TATAACGTCA TCCACGACAC GCTGTACTTG AGCCCGGATG CGGTCCAGGG TCTCACTTAC 
AGACTCTGCC ACATGTACTA CAATTTTTCG GGTATCATCC GAGTTCCTGC TCCTTGCCAT 
TATGCCCATA AACTGGCTTA CTTGGTGGGC CAGAGTCTTC ATCAACAACC ACACGAATCT 
CTGTCAAAAT CTCTCTTTTA CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PIWIL3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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