RTTN cDNA ORF clone, Bos taurus(cattle)

The following RTTN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RTTN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa229994 XM_024984409.1
Latest version!
Bos taurus rotatin (RTTN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa229993 XM_024984408.1
Latest version!
Bos taurus rotatin (RTTN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa36051 NM_001206207.1
Latest version!
Bos taurus rotatin (RTTN), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa32440 XM_005224039.4
Latest version!
Bos taurus rotatin (RTTN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
View Old Accession Versions >>
OBa32440 XM_005224039.3 Bos taurus rotatin (RTTN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa229994
    Clone ID Related Accession (Same CDS sequence) XM_024984409.1
    Accession Version XM_024984409.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5790bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rotatin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGTCCTGA CGGGGCTCAT CCGGAAACTC GGTCACCCGC TGGCCGAGAT CCGGGAGCGC 
    GCGCTCAGGA GTATTCTCTG CAAGCTCGAG CACAGCTTGG TCTGCGGCGC GGACCTGGCT
    TCGCACAGGC TGCTGTTTCT CCACTTGCTG GAATGGTTCA ATTTCCCGTC CGTCCCAGTG
    AAGGACGAGG TCCTGGGTCT GCTGAGCAGA CTGGTTAAGT ATCCTCTAGC AGTGCAGCAT
    TTGGTTGATC TTGGCGCGGT CGAATTCTTA TCTAAACTTC GCCCTAATGT GGAGCCGAAT
    CTACAGGCAG AAATTGATGG CATTCTGGAT GGACTTTTTA GTCTTCCTTC AGAAGTTCCT
    GAAATGTATT CTTCATCTTA CCAGACCAGT CAAACTGATT TGCTGCAACA ACCTGAAATC
    TTAACAGGAT ATTTTCCTCA AGACAAAAGT ACTTTGCAGC AGACAGAAAT ACCTTCACGA
    CCTGTGGCAG CAAATCCGAC CGCAAAGTGT TTGAAGTTTT CTACTTTCCC CTGGCTCCCC
    CTGACCGCAG CTGACAGACA CGTTCTCTCG TCCAACGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTGTAA TCTGGAACAC CTGTGAACTG TTGAAAGACG TTATCATGCA AGATTTTCCT
    GCTGAGATAT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGAG GAGACGGGAA GCATCGTCTG GCATTACAGT CAGTGTCCTG CCTGCAGCAG
    CTGTGCACGT GTTTAAGAAA CAGACTCAAT TTCCACAGAG ATCCAAGTTT CTTCTCTAAT
    AAACAAGATG CCGTGTCCCA GAACTCTTCC CTGTCTTACT GTCCTGATGC CAGGGCGGCT
    CCGCATTCCC AGAACCCGTC TCCCGGAAGC AGCAGCCCGC GGCCCTCCGT GGTGGGGCGC
    ACGGGCCAGC GGCCCCGGGG GGACGGCCAG GACTGGGACG CCGTGTCCTC CAGTGGGAGT
    AGCAGCCACG CTCACGTCAG CTCGCGGGCC TCTGCCCCCT CGCCTCTGGA CCTGGCGCCC
    GTGGACCTGC CGGAGCTGGC AGAGGACGCG CTGGAGCTGC AGCTGCAGCA GCTGAGCCTG
    CCCCAGCTCT GCGTCTCCCT CCTGGAGGCC GCCGCGCCCC TCCTGAGGAC AGGTAGTAGA
    CAGATGATAA TAAGAGTTCT GGAGTTGCTC GCAGAAGTTG TGGTGCTTAT TGGTGAAGCG
    ATTTCAGCAC ATCTGTGGGA TGACAGCAGT CTTTTTGCAA TAGATATGAA AGAGAAGCTG
    CTCGTGGTGT TGGCGTCCCT TGGAGACACC GTGTGCTACC ATAAAAGCTG TGTCAGTCTG
    GAGCAGCCGG AGGCGCTGGT CGTGCACCAC AGGATGGCCT TTGTCAGCAT CTCGCTGTTT
    GCTGTCCGGC TCCTGCAGAC GCTGCTCCCT GTGGAAAAGG CAAGCCGCTT TTTACCGGAG
    CCTATGTTAG CAGCATTATT TTTTATTTCT TTGGACATGC CTGTTTCTTT GGAATATCCA
    AACATTCACG AAGCTGTTGT GGCCTATTTG GAACAGCTGA ATTCTGAAAA CTACAGTATT
    TATAAACGAA CTGCAGAGGC TGCTTATTCA ATTGAGTGTA CCTGTAACTT CCTGTCTGAT
    GTTGGGAAGG AGGGAGAAAA GAATCTCTCA GAGTTAGTGG AGCTGGCAGA TCAAGCCTTA
    CGTAGCTTTT CCTACCATCA GCATTTCCCC CTAATAAAGG AAATCATCTC CATTTGTTCA
    AAAATCTGGA AATCCGTGCA GGCCAGTCCT TTACTACAAG GAGAAAGTCA GAAGGTGTTT
    CTGCGGATGT TGTCTCACCC TTTGCTACAA GTGAAAGTTG AAACTTACCA CTGCTGTCTG
    GAAATTGTTA AGGAATGTTT AGGCATCCAT AATATCAGTA AACCTGTGTC CTCCCTTTGT
    AATGGAATTC ATTTTCTACT TCACCCAAAA GTTCTGTATG AAATCTCTGC ATTTGGCATT
    CAAGACCCCA AAAATGAGGT GAGCGCCGCA GCCAAGGCCA TCCTGGTGCA CCTGCTGCAG
    GGCCGACTGA GGGTGTCGGC GCCGACCTGG AACAAGTTCA TCGAGTCCCT GTGCCCCGTC
    ATCCCCGTGC TGCAGGGCTA CGCCGACACT GAAGACCCTC TGGGGAACTG CATCCTCCAC
    CTCAGCAGAG AGAGTCCAGA GACCGATGAA GGGGTGCTCC CCTCTGCCAC CCGGCTGAAG
    TCCATGCTGC GGCTCCTGCT GGTGAAGAAG CCCTCGGTCC GGGTGCTGGC ATTAAAGCTC
    TTAGCATCTC ACCTCACAAG GGAAGAGGGG TCTGACACCA AGCGCCCTTC AATAGATGCC
    AGAGTTCTCT CCAGAGTTAC TAATTTATTT ATTGTGAAAA AACCTATTGA ACTCAAATTG
    GATGACCGAA GAGAACTGAT GATTAAGTTG GAGACTGTTG AAAAGGTGTA TGGAATCTTT
    GTCTCAGATG ATGTTGATCT TGTTTTGAGA AAGTCAGCCG CAGAGCAGTT AGTGGTGATT
    ATGCAAGATA TTAAAATGCA TGCTGTGGTG AAAAAAGTGC ACCTAGTTGA CAAGATAATT
    GAGTATTTAA GTGAATGTGT GGGTCAGGAT GGTGAGGTGG TGGAGTGTTT GGTTCAGCCG
    AGCCTCATGC TCCTGAGGAA GGTCCTCTGT GCCGACCCCG TCCTGCGAGA GTCTCTGTCC
    CAGAAGACTT CTGTCCTGAC CCTGCTCTTC AGAGTTTCCT TGATATTTCA TGAAGATTGT
    ACCATTGTGA CTGAAGTTGG AGCATTGTTT TGTCTTCTGT TATTTGATGA AGTATCAAGA
    ATGGATATGT GGTCTGATAG TCCTTCCTGC ACACCATCTT CACCATCAGT CTTCAGTCTG
    CCTGTTTCAG TTTCTAGAAG GTATCATCTA CCAGTGCATG TAGTTGGCCA CCATGCCGTG
    AGTCCTTACT CCATGGTTTT GCCGTTATCT GCTGACTCTT TGGCCTTGAA GCCGGTATCA
    GATATGCTGA GGATGGCTTG GAACCTGTCG TGGTATCACG GGAGTGACAA TGTGTTGAAG
    CAAGTGAACT CCGAAGCTGA AATCCAGGAA TTTTTAGATA CACTGAAGTT ATCCACCGAG
    GACACCCTTA CTCTGAAGAT GACCCACACA CCTAGTGGAC TCAAGGACTG TCTCTGCTCC
    ATTGTTCAGG CCGCAGGCCA CAGGGAAGTG AGGGCTGCCC TCACAAGGAT GAGTTTTTAT
    CTCCTAAATG ACAGACTGTC TTTCAAGGGC GGCACTGGCC CCTGTGGGGT TACCCTGAAG
    TCCTTGTCTT GGCACACCAC ACTAAACAGG TTTTTACAAG TTCTTCCTGC TTGCACTGAA
    GATGAGAAGC TGTTAATAGA TATCATACAT TTTCTCAGTA AGTTATTGAA GGAACAAAGG
    AAAAATGTAC CCGTAGAACT TCTAAACTGG CTTCTAGAAG TACTTCTTAG ACAAAGTCCA
    AATCCACTGT TGGACCTGCT GGTTCTGACG GAGTCCCAGA CACGAGAGGA AACAGATGAT
    GTCCGCACTG CGGTCAGGCA GCAGCTCCAG AAAGAGCTGA TTGCTCTCTT TAGTACCTTG
    CTGCTCAGTT TTGAGGCTGT TACTGACAGG AAATGCTTGG AGCTTTTTTA CGTTTTCCAA
    ACGCAGCTGG CCCTGAAACT ACTCCAGTGT CTGAGAGTCA CGGACGCTCC TCACTTCTAT
    GGCCTACCGT CCCTTGAGAG GACCTTGCGG GGGATGGCCC ACCTCACGGC GTGTCCAGGG
    TGGAGCACAC ACTCTCCTCT GACAAAGCCA CTCGACATTT GTGGGAAATA CTTGTCAGGT
    CTCCTCGAAG TCATCACATC CTTCTACGTG GAGCGAGGAG GAAATGCGAT GTCCTTCATG
    GGAAAAGGTG TCACAAAGAG CACCGTTCTC TGCCTGCTCC ACTTGTCGCA TGAGATGATG
    GCCCAGGCCC AGAGCTCGGG GTGGATGTCA CTTTGGTTCT TGCCTTTGGG GAGTCAGAGT
    GAAGACCATG CTCCCACTCA GCAGGGATTG GCTTGGCTGA TTCCGTTGTG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTAACCAC CCTTGAAACT
    GGCTGTGTGG CCTTGGCAAA CAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGAACGGTG
    GTGAACATTC TTCTGGACCA GTCGGAATGC AGCATGGTGC GCCGGGAGGC TGCATTTATT
    TTTCAGAATC TCCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGCCCCTGTG TCCATGATGA GGACTCTGGG CTGTCTCTCA TAGGAAAACC TGCCCTCCAG
    GCTCTTTTAT ACCACTGCCA TTTTTATGAA CATTTGAATC AGATGGTAAA ACATTGTTAC
    CTGGGACGGT ATGCATTTGA TTTCAGTTTT TCAGCTCTTA ATGGAGCTCC TGAAGGCAAT
    GATTTAAATG GTTTAGATGA CTCGTTCAAG TTCTGGAGGG CTCCATCTCG AATGTCCAGT
    CAAGATCGAG ATCCAAGTTC TCTCTCTACC TCGGAAACAA TGGTGGCAGC TTCTTCGGGC
    AGTGCTGAAT TTCAGTCCCT CGTGCTCTCC ATGGTGCCTC ACCCTGAAGT CACACACGAC
    CAGCTGGTGG CCCAAGGTCA GCGTGAAGCT GCATCTCCAC GGCTTCCTCC TGATTCACCC
    CTCTCTGCTC CTCTGCCCAG GCAGTGTGTG TTTGTCACCC CGCCCCTCCT CTCCGCGATG
    TGCAGCCTCC TAGACAGCCT CTTGGTGGCC ACTCCAGGCG ACACTGCCGG CGCTTTCCGA
    CGAACCCGTC TCATTGAGCT TCTCTGTAGC ATCGCAGTTG CCACCCTCCT AGAGACAAGT
    GTCCAGGAGC TCCGGGCCTC GCTGCCTACA TCTCTTCCTG CTGAACACAC TCAGGCTCAG
    GTGTCCTTTC TCCTGGAATA CTTGTCCTCC TTATCCCGGC TCCTGCAGTC ATGTTTATTG
    GTGGATCCCG ACCTTGTGAT TCAGGATGAG CTTCTGAAAC CTCTTATCCC AAACATCATC
    CGAGTTCTCA CCCTATGCAC CAAAGATATA TTAGATGTAG AGTTAAGGTC AGCTTTTTAT
    CAGACATGGA CTCATCTGTT CAACCTTCTG GCCGCACTGC TGAGGCGAGC TGGCCCTGCC
    TCTTTCCCAT ATGTTACCCT GGGTCTGGCC CGGTCTTGGG CAGCGGTGAT CGATACATTC
    TGCAGGTGTG CGCGTTTGTC TACCACAAGC CCTGCTCTGT ACACCGCCAG CCTGCAGTTC
    CTTTCTGTTC TCTTGACCGA AGAAGCAAAA AGGCGTCTCC AGGATGAGGA GAAAACAAGC
    TTATGCCACA GTCCAACAGT GGCTTCACTT CTTGACAAAA CCCAGGAAAG TCAGAACTCA
    CTAGAACGAC TCACTGAAGT CATTCTTCAG TGCTATGAGG GGAAAACCTC CAAAGATGTC
    CTGAAGAGGG TCGCTGCGAA CGCACTGATG TCGCTTCTGG CCGTCAGCAG AAGAGCACAG
    AGACATGCGC TGAAGGCCCA TCTTGTAGAC AGCTGCGTGG AGCAGATGAA GCACATTCAT
    GCACAGCTGA ACCTGGGTTC TCTGAAGCCG GGAAGGGCAG TGCTGAGGAA AAAGGTCACT
    GAGTTCTCAG TCTCTGGGAG GCAGGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840177.1
    CDS309..6098
    Translation

    Target ORF information:

    RefSeq Version XM_024984409.1
    Organism Bos taurus(cattle)
    Definition Bos taurus rotatin (RTTN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984409.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGTCCTGA CGGGGCTCAT CCGGAAACTC GGTCACCCGC TGGCCGAGAT CCGGGAGCGC 
    GCGCTCAGGA GTATTCTCTG CAAGCTCGAG CACAGCTTGG TCTGCGGCGC GGACCTGGCT
    TCGCACAGGC TGCTGTTTCT CCACTTGCTG GAATGGTTCA ATTTCCCGTC CGTCCCAGTG
    AAGGACGAGG TCCTGGGTCT GCTGAGCAGA CTGGTTAAGT ATCCTCTAGC AGTGCAGCAT
    TTGGTTGATC TTGGCGCGGT CGAATTCTTA TCTAAACTTC GCCCTAATGT GGAGCCGAAT
    CTACAGGCAG AAATTGATGG CATTCTGGAT GGACTTTTTA GTCTTCCTTC AGAAGTTCCT
    GAAATGTATT CTTCATCTTA CCAGACCAGT CAAACTGATT TGCTGCAACA ACCTGAAATC
    TTAACAGGAT ATTTTCCTCA AGACAAAAGT ACTTTGCAGC AGACAGAAAT ACCTTCACGA
    CCTGTGGCAG CAAATCCGAC CGCAAAGTGT TTGAAGTTTT CTACTTTCCC CTGGCTCCCC
    CTGACCGCAG CTGACAGACA CGTTCTCTCG TCCAACGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTGTAA TCTGGAACAC CTGTGAACTG TTGAAAGACG TTATCATGCA AGATTTTCCT
    GCTGAGATAT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGAG GAGACGGGAA GCATCGTCTG GCATTACAGT CAGTGTCCTG CCTGCAGCAG
    CTGTGCACGT GTTTAAGAAA CAGACTCAAT TTCCACAGAG ATCCAAGTTT CTTCTCTAAT
    AAACAAGATG CCGTGTCCCA GAACTCTTCC CTGTCTTACT GTCCTGATGC CAGGGCGGCT
    CCGCATTCCC AGAACCCGTC TCCCGGAAGC AGCAGCCCGC GGCCCTCCGT GGTGGGGCGC
    ACGGGCCAGC GGCCCCGGGG GGACGGCCAG GACTGGGACG CCGTGTCCTC CAGTGGGAGT
    AGCAGCCACG CTCACGTCAG CTCGCGGGCC TCTGCCCCCT CGCCTCTGGA CCTGGCGCCC
    GTGGACCTGC CGGAGCTGGC AGAGGACGCG CTGGAGCTGC AGCTGCAGCA GCTGAGCCTG
    CCCCAGCTCT GCGTCTCCCT CCTGGAGGCC GCCGCGCCCC TCCTGAGGAC AGGTAGTAGA
    CAGATGATAA TAAGAGTTCT GGAGTTGCTC GCAGAAGTTG TGGTGCTTAT TGGTGAAGCG
    ATTTCAGCAC ATCTGTGGGA TGACAGCAGT CTTTTTGCAA TAGATATGAA AGAGAAGCTG
    CTCGTGGTGT TGGCGTCCCT TGGAGACACC GTGTGCTACC ATAAAAGCTG TGTCAGTCTG
    GAGCAGCCGG AGGCGCTGGT CGTGCACCAC AGGATGGCCT TTGTCAGCAT CTCGCTGTTT
    GCTGTCCGGC TCCTGCAGAC GCTGCTCCCT GTGGAAAAGG CAAGCCGCTT TTTACCGGAG
    CCTATGTTAG CAGCATTATT TTTTATTTCT TTGGACATGC CTGTTTCTTT GGAATATCCA
    AACATTCACG AAGCTGTTGT GGCCTATTTG GAACAGCTGA ATTCTGAAAA CTACAGTATT
    TATAAACGAA CTGCAGAGGC TGCTTATTCA ATTGAGTGTA CCTGTAACTT CCTGTCTGAT
    GTTGGGAAGG AGGGAGAAAA GAATCTCTCA GAGTTAGTGG AGCTGGCAGA TCAAGCCTTA
    CGTAGCTTTT CCTACCATCA GCATTTCCCC CTAATAAAGG AAATCATCTC CATTTGTTCA
    AAAATCTGGA AATCCGTGCA GGCCAGTCCT TTACTACAAG GAGAAAGTCA GAAGGTGTTT
    CTGCGGATGT TGTCTCACCC TTTGCTACAA GTGAAAGTTG AAACTTACCA CTGCTGTCTG
    GAAATTGTTA AGGAATGTTT AGGCATCCAT AATATCAGTA AACCTGTGTC CTCCCTTTGT
    AATGGAATTC ATTTTCTACT TCACCCAAAA GTTCTGTATG AAATCTCTGC ATTTGGCATT
    CAAGACCCCA AAAATGAGGT GAGCGCCGCA GCCAAGGCCA TCCTGGTGCA CCTGCTGCAG
    GGCCGACTGA GGGTGTCGGC GCCGACCTGG AACAAGTTCA TCGAGTCCCT GTGCCCCGTC
    ATCCCCGTGC TGCAGGGCTA CGCCGACACT GAAGACCCTC TGGGGAACTG CATCCTCCAC
    CTCAGCAGAG AGAGTCCAGA GACCGATGAA GGGGTGCTCC CCTCTGCCAC CCGGCTGAAG
    TCCATGCTGC GGCTCCTGCT GGTGAAGAAG CCCTCGGTCC GGGTGCTGGC ATTAAAGCTC
    TTAGCATCTC ACCTCACAAG GGAAGAGGGG TCTGACACCA AGCGCCCTTC AATAGATGCC
    AGAGTTCTCT CCAGAGTTAC TAATTTATTT ATTGTGAAAA AACCTATTGA ACTCAAATTG
    GATGACCGAA GAGAACTGAT GATTAAGTTG GAGACTGTTG AAAAGGTGTA TGGAATCTTT
    GTCTCAGATG ATGTTGATCT TGTTTTGAGA AAGTCAGCCG CAGAGCAGTT AGTGGTGATT
    ATGCAAGATA TTAAAATGCA TGCTGTGGTG AAAAAAGTGC ACCTAGTTGA CAAGATAATT
    GAGTATTTAA GTGAATGTGT GGGTCAGGAT GGTGAGGTGG TGGAGTGTTT GGTTCAGCCG
    AGCCTCATGC TCCTGAGGAA GGTCCTCTGT GCCGACCCCG TCCTGCGAGA GTCTCTGTCC
    CAGAAGACTT CTGTCCTGAC CCTGCTCTTC AGAGTTTCCT TGATATTTCA TGAAGATTGT
    ACCATTGTGA CTGAAGTTGG AGCATTGTTT TGTCTTCTGT TATTTGATGA AGTATCAAGA
    ATGGATATGT GGTCTGATAG TCCTTCCTGC ACACCATCTT CACCATCAGT CTTCAGTCTG
    CCTGTTTCAG TTTCTAGAAG GTATCATCTA CCAGTGCATG TAGTTGGCCA CCATGCCGTG
    AGTCCTTACT CCATGGTTTT GCCGTTATCT GCTGACTCTT TGGCCTTGAA GCCGGTATCA
    GATATGCTGA GGATGGCTTG GAACCTGTCG TGGTATCACG GGAGTGACAA TGTGTTGAAG
    CAAGTGAACT CCGAAGCTGA AATCCAGGAA TTTTTAGATA CACTGAAGTT ATCCACCGAG
    GACACCCTTA CTCTGAAGAT GACCCACACA CCTAGTGGAC TCAAGGACTG TCTCTGCTCC
    ATTGTTCAGG CCGCAGGCCA CAGGGAAGTG AGGGCTGCCC TCACAAGGAT GAGTTTTTAT
    CTCCTAAATG ACAGACTGTC TTTCAAGGGC GGCACTGGCC CCTGTGGGGT TACCCTGAAG
    TCCTTGTCTT GGCACACCAC ACTAAACAGG TTTTTACAAG TTCTTCCTGC TTGCACTGAA
    GATGAGAAGC TGTTAATAGA TATCATACAT TTTCTCAGTA AGTTATTGAA GGAACAAAGG
    AAAAATGTAC CCGTAGAACT TCTAAACTGG CTTCTAGAAG TACTTCTTAG ACAAAGTCCA
    AATCCACTGT TGGACCTGCT GGTTCTGACG GAGTCCCAGA CACGAGAGGA AACAGATGAT
    GTCCGCACTG CGGTCAGGCA GCAGCTCCAG AAAGAGCTGA TTGCTCTCTT TAGTACCTTG
    CTGCTCAGTT TTGAGGCTGT TACTGACAGG AAATGCTTGG AGCTTTTTTA CGTTTTCCAA
    ACGCAGCTGG CCCTGAAACT ACTCCAGTGT CTGAGAGTCA CGGACGCTCC TCACTTCTAT
    GGCCTACCGT CCCTTGAGAG GACCTTGCGG GGGATGGCCC ACCTCACGGC GTGTCCAGGG
    TGGAGCACAC ACTCTCCTCT GACAAAGCCA CTCGACATTT GTGGGAAATA CTTGTCAGGT
    CTCCTCGAAG TCATCACATC CTTCTACGTG GAGCGAGGAG GAAATGCGAT GTCCTTCATG
    GGAAAAGGTG TCACAAAGAG CACCGTTCTC TGCCTGCTCC ACTTGTCGCA TGAGATGATG
    GCCCAGGCCC AGAGCTCGGG GTGGATGTCA CTTTGGTTCT TGCCTTTGGG GAGTCAGAGT
    GAAGACCATG CTCCCACTCA GCAGGGATTG GCTTGGCTGA TTCCGTTGTG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTAACCAC CCTTGAAACT
    GGCTGTGTGG CCTTGGCAAA CAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGAACGGTG
    GTGAACATTC TTCTGGACCA GTCGGAATGC AGCATGGTGC GCCGGGAGGC TGCATTTATT
    TTTCAGAATC TCCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGCCCCTGTG TCCATGATGA GGACTCTGGG CTGTCTCTCA TAGGAAAACC TGCCCTCCAG
    GCTCTTTTAT ACCACTGCCA TTTTTATGAA CATTTGAATC AGATGGTAAA ACATTGTTAC
    CTGGGACGGT ATGCATTTGA TTTCAGTTTT TCAGCTCTTA ATGGAGCTCC TGAAGGCAAT
    GATTTAAATG GTTTAGATGA CTCGTTCAAG TTCTGGAGGG CTCCATCTCG AATGTCCAGT
    CAAGATCGAG ATCCAAGTTC TCTCTCTACC TCGGAAACAA TGGTGGCAGC TTCTTCGGGC
    AGTGCTGAAT TTCAGTCCCT CGTGCTCTCC ATGGTGCCTC ACCCTGAAGT CACACACGAC
    CAGCTGGTGG CCCAAGGTCA GCGTGAAGCT GCATCTCCAC GGCTTCCTCC TGATTCACCC
    CTCTCTGCTC CTCTGCCCAG GCAGTGTGTG TTTGTCACCC CGCCCCTCCT CTCCGCGATG
    TGCAGCCTCC TAGACAGCCT CTTGGTGGCC ACTCCAGGCG ACACTGCCGG CGCTTTCCGA
    CGAACCCGTC TCATTGAGCT TCTCTGTAGC ATCGCAGTTG CCACCCTCCT AGAGACAAGT
    GTCCAGGAGC TCCGGGCCTC GCTGCCTACA TCTCTTCCTG CTGAACACAC TCAGGCTCAG
    GTGTCCTTTC TCCTGGAATA CTTGTCCTCC TTATCCCGGC TCCTGCAGTC ATGTTTATTG
    GTGGATCCCG ACCTTGTGAT TCAGGATGAG CTTCTGAAAC CTCTTATCCC AAACATCATC
    CGAGTTCTCA CCCTATGCAC CAAAGATATA TTAGATGTAG AGTTAAGGTC AGCTTTTTAT
    CAGACATGGA CTCATCTGTT CAACCTTCTG GCCGCACTGC TGAGGCGAGC TGGCCCTGCC
    TCTTTCCCAT ATGTTACCCT GGGTCTGGCC CGGTCTTGGG CAGCGGTGAT CGATACATTC
    TGCAGGTGTG CGCGTTTGTC TACCACAAGC CCTGCTCTGT ACACCGCCAG CCTGCAGTTC
    CTTTCTGTTC TCTTGACCGA AGAAGCAAAA AGGCGTCTCC AGGATGAGGA GAAAACAAGC
    TTATGCCACA GTCCAACAGT GGCTTCACTT CTTGACAAAA CCCAGGAAAG TCAGAACTCA
    CTAGAACGAC TCACTGAAGT CATTCTTCAG TGCTATGAGG GGAAAACCTC CAAAGATGTC
    CTGAAGAGGG TCGCTGCGAA CGCACTGATG TCGCTTCTGG CCGTCAGCAG AAGAGCACAG
    AGACATGCGC TGAAGGCCCA TCTTGTAGAC AGCTGCGTGG AGCAGATGAA GCACATTCAT
    GCACAGCTGA ACCTGGGTTC TCTGAAGCCG GGAAGGGCAG TGCTGAGGAA AAAGGTCACT
    GAGTTCTCAG TCTCTGGGAG GCAGGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229993
    Clone ID Related Accession (Same CDS sequence) XM_024984408.1
    Accession Version XM_024984408.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6045bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rotatin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCAAGATT TTCCTGCTGA GATATTCCTT CAAAGGCCAA AAATTGTTCA GAGCCTTTTA 
    TCTCTGTTGA AACTGGCCTT TGGAGGAGAC GGGAAGCATC GTCTGGCATT ACAGTCAGTG
    TCCTGCCTGC AGCAGCTGTG CACGTGTTTA AGAAACAGAC TCAATTTCCA CAGAGATCCA
    AGTTTCTTCT CTAATAAACA AGATGCCGTG TCCCAGAACT CTTCCCTGTC TTACTGTCCT
    GATGCCAGGG CGGCTCCGCA TTCCCAGAAC CCGTCTCCCG GAAGCAGCAG CCCGCGGCCC
    TCCGTGGTGG GGCGCACGGG CCAGCGGCCC CGGGGGGACG GCCAGGACTG GGACGCCGTG
    TCCTCCAGTG GGAGTAGCAG CCACGCTCAC GTCAGCTCGC GGGCCTCTGC CCCCTCGCCT
    CTGGACCTGG CGCCCGTGGA CCTGCCGGAG CTGGCAGAGG ACGCGCTGGA GCTGCAGCTG
    CAGCAGCTGA GCCTGCCCCA GCTCTGCGTC TCCCTCCTGG AGGCCGCCGC GCCCCTCCTG
    AGGACAGGTA GTAGACAGAT GATAATAAGA GTTCTGGAGT TGCTCGCAGA AGTTGTGGTG
    CTTATTGGTG AAGCGATTTC AGCACATCTG TGGGATGACA GCAGTCTTTT TGCAATAGAT
    ATGAAAGAGA AGCTGCTCGT GGTGTTGGCG TCCCTTGGAG ACACCGTGTG CTACCATAAA
    AGCTGTGTCA GTCTGGAGCA GCCGGAGGCG CTGGTCGTGC ACCACAGGAT GGCCTTTGTC
    AGCATCTCGC TGTTTGCTGT CCGGCTCCTG CAGACGCTGC TCCCTGTGGA AAAGGCAAGC
    CGCTTTTTAC CGGAGCCTAT GTTAGCAGCA TTATTTTTTA TTTCTTTGGA CATGCCTGTT
    TCTTTGGAAT ATCCAAACAT TCACGAAGCT GTTGTGGCCT ATTTGGAACA GCTGAATTCT
    GAAAACTACA GTATTTATAA ACGAACTGCA GAGGCTGCTT ATTCAATTGA GTGTACCTGT
    AACTTCCTGT CTGATGTTGG GAAGGAGGGA GAAAAGAATC TCTCAGAGTT AGTGGAGCTG
    GCAGATCAAG CCTTACGTAG CTTTTCCTAC CATCAGCATT TCCCCCTAAT AAAGGAAATC
    ATCTCCATTT GTTCAAAAAT CTGGAAATCC GTGCAGGCCA GTCCTTTACT ACAAGGAGAA
    AGTCAGAAGG TGTTTCTGCG GATGTTGTCT CACCCTTTGC TACAAGTGAA AGTTGAAACT
    TACCACTGCT GTCTGGAAAT TGTTAAGGAA TGTTTAGGCA TCCATAATAT CAGTAAACCT
    GTGTCCTCCC TTTGTAATGG AATTCATTTT CTACTTCACC CAAAAGTTCT GTATGAAATC
    TCTGCATTTG GCATTCAAGA CCCCAAAAAT GAGGTGAGCG CCGCAGCCAA GGCCATCCTG
    GTGCACCTGC TGCAGGGCCG ACTGAGGGTG TCGGCGCCGA CCTGGAACAA GTTCATCGAG
    TCCCTGTGCC CCGTCATCCC CGTGCTGCAG GGCTACGCCG ACACTGAAGA CCCTCTGGGG
    AACTGCATCC TCCACCTCAG CAGAGAGAGT CCAGAGACCG ATGAAGGGGT GCTCCCCTCT
    GCCACCCGGC TGAAGTCCAT GCTGCGGCTC CTGCTGGTGA AGAAGCCCTC GGTCCGGGTG
    CTGGCATTAA AGCTCTTAGC ATCTCACCTC ACAAGGGAAG AGGGGTCTGA CACCAAGCGC
    CCTTCAATAG ATGCCAGAGT TCTCTCCAGA GTTACTAATT TATTTATTGT GAAAAAACCT
    ATTGAACTCA AATTGGATGA CCGAAGAGAA CTGATGATTA AGTTGGAGAC TGTTGAAAAG
    GTGTATGGAA TCTTTGTCTC AGATGATGTT GATCTTGTTT TGAGAAAGTC AGCCGCAGAG
    CAGTTAGTGG TGATTATGCA AGATATTAAA ATGCATGCTG TGGTGAAAAA AGTGCACCTA
    GTTGACAAGA TAATTGAGTA TTTAAGTGAA TGTGTGGGTC AGGATGGTGA GGTGGTGGAG
    TGTTTGGTTC AGCCGAGCCT CATGCTCCTG AGGAAGGTCC TCTGTGCCGA CCCCGTCCTG
    CGAGAGTCTC TGTCCCAGAA GACTTCTGTC CTGACCCTGC TCTTCAGAGT TTCCTTGATA
    TTTCATGAAG ATTGTACCAT TGTGACTGAA GTTGGAGCAT TGTTTTGTCT TCTGTTATTT
    GATGAAGTAT CAAGAATGGA TATGTGGTCT GATAGTCCTT CCTGCACACC ATCTTCACCA
    TCAGTCTTCA GTCTGCCTGT TTCAGTTTCT AGAAGGTATC ATCTACCAGT GCATGTAGTT
    GGCCACCATG CCGTGAGTCC TTACTCCATG GTTTTGCCGT TATCTGCTGA CTCTTTGGCC
    TTGAAGCCGG TATCAGATAT GCTGAGGATG GCTTGGAACC TGTCGTGGTA TCACGGGAGT
    GACAATGTGT TGAAGCAAGT GAACTCCGAA GCTGAAATCC AGGAATTTTT AGATACACTG
    AAGTTATCCA CCGAGGACAC CCTTACTCTG AAGATGACCC ACACACCTAG TGGACTCAAG
    GACTGTCTCT GCTCCATTGT TCAGGCCGCA GGCCACAGGG AAGTGAGGGC TGCCCTCACA
    AGGATGAGTT TTTATCTCCT AAATGACAGA CTGTCTTTCA AGGGCGGCAC TGGCCCCTGT
    GGGGTTACCC TGAAGTCCTT GTCTTGGCAC ACCACACTAA ACAGGTTTTT ACAAGTTCTT
    CCTGCTTGCA CTGAAGATGA GAAGCTGTTA ATAGATATCA TACATTTTCT CAGTAAGTTA
    TTGAAGGAAC AAAGGAAAAA TGTACCCGTA GAACTTCTAA ACTGGCTTCT AGAAGTACTT
    CTTAGACAAA GTCCAAATCC ACTGTTGGAC CTGCTGGTTC TGACGGAGTC CCAGACACGA
    GAGGAAACAG ATGATGTCCG CACTGCGGTC AGGCAGCAGC TCCAGAAAGA GCTGATTGCT
    CTCTTTAGTA CCTTGCTGCT CAGTTTTGAG GCTGTTACTG ACAGGAAATG CTTGGAGCTT
    TTTTACGTTT TCCAAACGCA GCTGGCCCTG AAACTACTCC AGTGTCTGAG AGTCACGGAC
    GCTCCTCACT TCTATGGCCT ACCGTCCCTT GAGAGGACCT TGCGGGGGAT GGCCCACCTC
    ACGGCGTGTC CAGGGTGGAG CACACACTCT CCTCTGACAA AGCCACTCGA CATTTGTGGG
    AAATACTTGT CAGGTCTCCT CGAAGTCATC ACATCCTTCT ACGTGGAGCG AGGAGGAAAT
    GCGATGTCCT TCATGGGAAA AGGTGTCACA AAGAGCACCG TTCTCTGCCT GCTCCACTTG
    TCGCATGAGA TGATGGCCCA GGCCCAGAGC TCGGGGTGGA TGTCACTTTG GTTCTTGCCT
    TTGGGGAGTC AGAGTGAAGA CCATGCTCCC ACTCAGCAGG GATTGGCTTG GCTGATTCCG
    TTGTGGGTTG ACCGAGACCC AGAGGTGAGG TTCACTTCAT TGGGATTAGG ATCAGCACTA
    ACCACCCTTG AAACTGGCTG TGTGGCCTTG GCAAACAGTT GTCAAAACAT TTCTGGTGGG
    CTCTGGGGAA CGGTGGTGAA CATTCTTCTG GACCAGTCGG AATGCAGCAT GGTGCGCCGG
    GAGGCTGCAT TTATTTTTCA GAATCTCCTT GTAATTCCAA TGCCTTCAGT AATTATAAAG
    GATTATACTT GGCAGGGCCC CTGTGTCCAT GATGAGGACT CTGGGCTGTC TCTCATAGGA
    AAACCTGCCC TCCAGGCTCT TTTATACCAC TGCCATTTTT ATGAACATTT GAATCAGATG
    GTAAAACATT GTTACCTGGG ACGGTATGCA TTTGATTTCA GTTTTTCAGC TCTTAATGGA
    GCTCCTGAAG GCAATGATTT AAATGGTTTA GATGACTCGT TCAAGTTCTG GAGGGCTCCA
    TCTCGAATGT CCAGTCAAGA TCGAGATCCA AGTTCTCTCT CTACCTCGGA AACAATGGTG
    GCAGCTTCTT CGGGCAGTGC TGAATTTCAG TCCCTCGTGC TCTCCATGGT GCCTCACCCT
    GAAGTCACAC ACGACCAGCT GGTGGCCCAA GGTCAGCGTG AAGCTGCATC TCCACGGCTT
    CCTCCTGATT CACCCCTCTC TGCTCCTCTG CCCAGGCAGT GTGTGTTTGT CACCCCGCCC
    CTCCTCTCCG CGATGTGCAG CCTCCTAGAC AGCCTCTTGG TGGCCACTCC AGGCGACACT
    GCCGGCGCTT TCCGACGAAC CCGTCTCATT GAGCTTCTCT GTAGCATCGC AGTTGCCACC
    CTCCTAGAGA CAAGTGTCCA GGAGCTCCGG GCCTCGCTGC CTACATCTCT TCCTGCTGAA
    CACACTCAGG CTCAGGTGTC CTTTCTCCTG GAATACTTGT CCTCCTTATC CCGGCTCCTG
    CAGTCATGTT TATTGGTGGA TCCCGACCTT GTGATTCAGG ATGAGCTTCT GAAACCTCTT
    ATCCCAAACA TCATCCGAGT TCTCACCCTA TGCACCAAAG ATATATTAGA TGTAGAGTTA
    AGGTCAGCTT TTTATCAGAC ATGGACTCAT CTGTTCAACC TTCTGGCCGC ACTGCTGAGG
    CGAGCTGGCC CTGCCTCTTT CCCATATGTT ACCCTGGGTC TGGCCCGGTC TTGGGCAGCG
    GTGATCGATA CATTCTGCAG GTGTGCGCGT TTGTCTACCA CAAGCCCTGC TCTGTACACC
    GCCAGCCTGC AGTTCCTTTC TGTTCTCTTG ACCGAAGAAG CAAAAAGGCG TCTCCAGGAT
    GAGGAGAAAA CAAGCTTATG CCACAGTCCA ACAGTGGCTT CACTTCTTGA CAAAACCCAG
    GAAAGTCAGA ACTCACTAGA ACGACTCACT GAAGTCATTC TTCAGTGCTA TGAGGGGAAA
    ACCTCCAAAG ATGTCCTGAA GAGGGTCGCT GCGAACGCAC TGATGTCGCT TCTGGCCGTC
    AGCAGAAGAG CACAGAGACA TGCGCTGAAG GCCCATCTTG TAGACAGCTG CGTGGAGCAG
    ATGAAGCACA TTCATGCACA GCTGAACCTG GGTTCTCTGA AGCCGGGAAG GGCAGTGCTG
    AGGAAAAAGG AGGATGGTTT TATTAAAGAA TTAAGCATTG CCATGCAGCT CCTAAGAAAC
    TGTCTTTATC AGAATGAAGA ATGTAAAGGG GCGGCTCTGG ACGCTCACCT GGCCCCTGTC
    CTGCACGCTC TCTGGCCTTG GCTCTTGATG GATGACTCAC TGATGCAGGT TGCGCTGCAG
    CTGCTTTGTG TCTACACCGC AAATTTCCCA AATGGTTGCA GTTCCCTCTG TTGGTCAAGT
    TATGGACAGC ATCCTGTTCA AGCCGCACAC CGAGGGGCCC CCGGCAGCTC CCTGATGCTG
    TGTATCCTGA AGATGGCTTC CCAGGTGCCT CCAGAGAACA CGGCTGTTCA GCAGGCAGTG
    TTCATGATTC TTTCAAACCT GGCCTTGTCT CATGACTGTA AAGGGGTAAT TCAGAAAAGT
    AACTTCCTGC AGAACTTTCT GTCTCTGACG TTGCCGAAAG GGGGAAACAA ACATCTGAGT
    AACCTGAGCG TGCTCTGGCT GAAGCTGCTA CTGAACATGT CCTTCGAAGA AGATGGGCAG
    CAGGTGATCC TGCGGCTGGA CGGCTGTCTA GACCTGCTCA CAGAGATGAG CAGGTTCAAG
    CACAAGAGCA GCCTCTCCAT CCCCCTTCTT ATTTTCCATA ACATCTGCTT CAGTCCTGCC
    AACAAGCCCA AGATCCTGGC TAACGAGAAA GTCATTAGTG TGCTCGCTGC CTGTCTGGAA
    AGTGAGAATC AAAACGCTCA GAGGATTGGA GCAGCCGCAC TGTGGGCTCT CATTCACAAT
    TACCAGAAGG CAAAAACAAC TTTGAAAAAC CCATCAATAA AAAGAAGAGT GGAAGAAGCA
    TATTCCTTAG CAAAGAAAAC TTTCTCAGAC TCAGAAGAAA CCCCTCTAAG TGCCTATTAT
    TTGAAATGTC TTGAAAACCT TGTGCAGCAG CTTAACTCTT CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840176.1
    CDS387..6431
    Translation

    Target ORF information:

    RefSeq Version XM_024984408.1
    Organism Bos taurus(cattle)
    Definition Bos taurus rotatin (RTTN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984408.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCAAGATT TTCCTGCTGA GATATTCCTT CAAAGGCCAA AAATTGTTCA GAGCCTTTTA 
    TCTCTGTTGA AACTGGCCTT TGGAGGAGAC GGGAAGCATC GTCTGGCATT ACAGTCAGTG
    TCCTGCCTGC AGCAGCTGTG CACGTGTTTA AGAAACAGAC TCAATTTCCA CAGAGATCCA
    AGTTTCTTCT CTAATAAACA AGATGCCGTG TCCCAGAACT CTTCCCTGTC TTACTGTCCT
    GATGCCAGGG CGGCTCCGCA TTCCCAGAAC CCGTCTCCCG GAAGCAGCAG CCCGCGGCCC
    TCCGTGGTGG GGCGCACGGG CCAGCGGCCC CGGGGGGACG GCCAGGACTG GGACGCCGTG
    TCCTCCAGTG GGAGTAGCAG CCACGCTCAC GTCAGCTCGC GGGCCTCTGC CCCCTCGCCT
    CTGGACCTGG CGCCCGTGGA CCTGCCGGAG CTGGCAGAGG ACGCGCTGGA GCTGCAGCTG
    CAGCAGCTGA GCCTGCCCCA GCTCTGCGTC TCCCTCCTGG AGGCCGCCGC GCCCCTCCTG
    AGGACAGGTA GTAGACAGAT GATAATAAGA GTTCTGGAGT TGCTCGCAGA AGTTGTGGTG
    CTTATTGGTG AAGCGATTTC AGCACATCTG TGGGATGACA GCAGTCTTTT TGCAATAGAT
    ATGAAAGAGA AGCTGCTCGT GGTGTTGGCG TCCCTTGGAG ACACCGTGTG CTACCATAAA
    AGCTGTGTCA GTCTGGAGCA GCCGGAGGCG CTGGTCGTGC ACCACAGGAT GGCCTTTGTC
    AGCATCTCGC TGTTTGCTGT CCGGCTCCTG CAGACGCTGC TCCCTGTGGA AAAGGCAAGC
    CGCTTTTTAC CGGAGCCTAT GTTAGCAGCA TTATTTTTTA TTTCTTTGGA CATGCCTGTT
    TCTTTGGAAT ATCCAAACAT TCACGAAGCT GTTGTGGCCT ATTTGGAACA GCTGAATTCT
    GAAAACTACA GTATTTATAA ACGAACTGCA GAGGCTGCTT ATTCAATTGA GTGTACCTGT
    AACTTCCTGT CTGATGTTGG GAAGGAGGGA GAAAAGAATC TCTCAGAGTT AGTGGAGCTG
    GCAGATCAAG CCTTACGTAG CTTTTCCTAC CATCAGCATT TCCCCCTAAT AAAGGAAATC
    ATCTCCATTT GTTCAAAAAT CTGGAAATCC GTGCAGGCCA GTCCTTTACT ACAAGGAGAA
    AGTCAGAAGG TGTTTCTGCG GATGTTGTCT CACCCTTTGC TACAAGTGAA AGTTGAAACT
    TACCACTGCT GTCTGGAAAT TGTTAAGGAA TGTTTAGGCA TCCATAATAT CAGTAAACCT
    GTGTCCTCCC TTTGTAATGG AATTCATTTT CTACTTCACC CAAAAGTTCT GTATGAAATC
    TCTGCATTTG GCATTCAAGA CCCCAAAAAT GAGGTGAGCG CCGCAGCCAA GGCCATCCTG
    GTGCACCTGC TGCAGGGCCG ACTGAGGGTG TCGGCGCCGA CCTGGAACAA GTTCATCGAG
    TCCCTGTGCC CCGTCATCCC CGTGCTGCAG GGCTACGCCG ACACTGAAGA CCCTCTGGGG
    AACTGCATCC TCCACCTCAG CAGAGAGAGT CCAGAGACCG ATGAAGGGGT GCTCCCCTCT
    GCCACCCGGC TGAAGTCCAT GCTGCGGCTC CTGCTGGTGA AGAAGCCCTC GGTCCGGGTG
    CTGGCATTAA AGCTCTTAGC ATCTCACCTC ACAAGGGAAG AGGGGTCTGA CACCAAGCGC
    CCTTCAATAG ATGCCAGAGT TCTCTCCAGA GTTACTAATT TATTTATTGT GAAAAAACCT
    ATTGAACTCA AATTGGATGA CCGAAGAGAA CTGATGATTA AGTTGGAGAC TGTTGAAAAG
    GTGTATGGAA TCTTTGTCTC AGATGATGTT GATCTTGTTT TGAGAAAGTC AGCCGCAGAG
    CAGTTAGTGG TGATTATGCA AGATATTAAA ATGCATGCTG TGGTGAAAAA AGTGCACCTA
    GTTGACAAGA TAATTGAGTA TTTAAGTGAA TGTGTGGGTC AGGATGGTGA GGTGGTGGAG
    TGTTTGGTTC AGCCGAGCCT CATGCTCCTG AGGAAGGTCC TCTGTGCCGA CCCCGTCCTG
    CGAGAGTCTC TGTCCCAGAA GACTTCTGTC CTGACCCTGC TCTTCAGAGT TTCCTTGATA
    TTTCATGAAG ATTGTACCAT TGTGACTGAA GTTGGAGCAT TGTTTTGTCT TCTGTTATTT
    GATGAAGTAT CAAGAATGGA TATGTGGTCT GATAGTCCTT CCTGCACACC ATCTTCACCA
    TCAGTCTTCA GTCTGCCTGT TTCAGTTTCT AGAAGGTATC ATCTACCAGT GCATGTAGTT
    GGCCACCATG CCGTGAGTCC TTACTCCATG GTTTTGCCGT TATCTGCTGA CTCTTTGGCC
    TTGAAGCCGG TATCAGATAT GCTGAGGATG GCTTGGAACC TGTCGTGGTA TCACGGGAGT
    GACAATGTGT TGAAGCAAGT GAACTCCGAA GCTGAAATCC AGGAATTTTT AGATACACTG
    AAGTTATCCA CCGAGGACAC CCTTACTCTG AAGATGACCC ACACACCTAG TGGACTCAAG
    GACTGTCTCT GCTCCATTGT TCAGGCCGCA GGCCACAGGG AAGTGAGGGC TGCCCTCACA
    AGGATGAGTT TTTATCTCCT AAATGACAGA CTGTCTTTCA AGGGCGGCAC TGGCCCCTGT
    GGGGTTACCC TGAAGTCCTT GTCTTGGCAC ACCACACTAA ACAGGTTTTT ACAAGTTCTT
    CCTGCTTGCA CTGAAGATGA GAAGCTGTTA ATAGATATCA TACATTTTCT CAGTAAGTTA
    TTGAAGGAAC AAAGGAAAAA TGTACCCGTA GAACTTCTAA ACTGGCTTCT AGAAGTACTT
    CTTAGACAAA GTCCAAATCC ACTGTTGGAC CTGCTGGTTC TGACGGAGTC CCAGACACGA
    GAGGAAACAG ATGATGTCCG CACTGCGGTC AGGCAGCAGC TCCAGAAAGA GCTGATTGCT
    CTCTTTAGTA CCTTGCTGCT CAGTTTTGAG GCTGTTACTG ACAGGAAATG CTTGGAGCTT
    TTTTACGTTT TCCAAACGCA GCTGGCCCTG AAACTACTCC AGTGTCTGAG AGTCACGGAC
    GCTCCTCACT TCTATGGCCT ACCGTCCCTT GAGAGGACCT TGCGGGGGAT GGCCCACCTC
    ACGGCGTGTC CAGGGTGGAG CACACACTCT CCTCTGACAA AGCCACTCGA CATTTGTGGG
    AAATACTTGT CAGGTCTCCT CGAAGTCATC ACATCCTTCT ACGTGGAGCG AGGAGGAAAT
    GCGATGTCCT TCATGGGAAA AGGTGTCACA AAGAGCACCG TTCTCTGCCT GCTCCACTTG
    TCGCATGAGA TGATGGCCCA GGCCCAGAGC TCGGGGTGGA TGTCACTTTG GTTCTTGCCT
    TTGGGGAGTC AGAGTGAAGA CCATGCTCCC ACTCAGCAGG GATTGGCTTG GCTGATTCCG
    TTGTGGGTTG ACCGAGACCC AGAGGTGAGG TTCACTTCAT TGGGATTAGG ATCAGCACTA
    ACCACCCTTG AAACTGGCTG TGTGGCCTTG GCAAACAGTT GTCAAAACAT TTCTGGTGGG
    CTCTGGGGAA CGGTGGTGAA CATTCTTCTG GACCAGTCGG AATGCAGCAT GGTGCGCCGG
    GAGGCTGCAT TTATTTTTCA GAATCTCCTT GTAATTCCAA TGCCTTCAGT AATTATAAAG
    GATTATACTT GGCAGGGCCC CTGTGTCCAT GATGAGGACT CTGGGCTGTC TCTCATAGGA
    AAACCTGCCC TCCAGGCTCT TTTATACCAC TGCCATTTTT ATGAACATTT GAATCAGATG
    GTAAAACATT GTTACCTGGG ACGGTATGCA TTTGATTTCA GTTTTTCAGC TCTTAATGGA
    GCTCCTGAAG GCAATGATTT AAATGGTTTA GATGACTCGT TCAAGTTCTG GAGGGCTCCA
    TCTCGAATGT CCAGTCAAGA TCGAGATCCA AGTTCTCTCT CTACCTCGGA AACAATGGTG
    GCAGCTTCTT CGGGCAGTGC TGAATTTCAG TCCCTCGTGC TCTCCATGGT GCCTCACCCT
    GAAGTCACAC ACGACCAGCT GGTGGCCCAA GGTCAGCGTG AAGCTGCATC TCCACGGCTT
    CCTCCTGATT CACCCCTCTC TGCTCCTCTG CCCAGGCAGT GTGTGTTTGT CACCCCGCCC
    CTCCTCTCCG CGATGTGCAG CCTCCTAGAC AGCCTCTTGG TGGCCACTCC AGGCGACACT
    GCCGGCGCTT TCCGACGAAC CCGTCTCATT GAGCTTCTCT GTAGCATCGC AGTTGCCACC
    CTCCTAGAGA CAAGTGTCCA GGAGCTCCGG GCCTCGCTGC CTACATCTCT TCCTGCTGAA
    CACACTCAGG CTCAGGTGTC CTTTCTCCTG GAATACTTGT CCTCCTTATC CCGGCTCCTG
    CAGTCATGTT TATTGGTGGA TCCCGACCTT GTGATTCAGG ATGAGCTTCT GAAACCTCTT
    ATCCCAAACA TCATCCGAGT TCTCACCCTA TGCACCAAAG ATATATTAGA TGTAGAGTTA
    AGGTCAGCTT TTTATCAGAC ATGGACTCAT CTGTTCAACC TTCTGGCCGC ACTGCTGAGG
    CGAGCTGGCC CTGCCTCTTT CCCATATGTT ACCCTGGGTC TGGCCCGGTC TTGGGCAGCG
    GTGATCGATA CATTCTGCAG GTGTGCGCGT TTGTCTACCA CAAGCCCTGC TCTGTACACC
    GCCAGCCTGC AGTTCCTTTC TGTTCTCTTG ACCGAAGAAG CAAAAAGGCG TCTCCAGGAT
    GAGGAGAAAA CAAGCTTATG CCACAGTCCA ACAGTGGCTT CACTTCTTGA CAAAACCCAG
    GAAAGTCAGA ACTCACTAGA ACGACTCACT GAAGTCATTC TTCAGTGCTA TGAGGGGAAA
    ACCTCCAAAG ATGTCCTGAA GAGGGTCGCT GCGAACGCAC TGATGTCGCT TCTGGCCGTC
    AGCAGAAGAG CACAGAGACA TGCGCTGAAG GCCCATCTTG TAGACAGCTG CGTGGAGCAG
    ATGAAGCACA TTCATGCACA GCTGAACCTG GGTTCTCTGA AGCCGGGAAG GGCAGTGCTG
    AGGAAAAAGG AGGATGGTTT TATTAAAGAA TTAAGCATTG CCATGCAGCT CCTAAGAAAC
    TGTCTTTATC AGAATGAAGA ATGTAAAGGG GCGGCTCTGG ACGCTCACCT GGCCCCTGTC
    CTGCACGCTC TCTGGCCTTG GCTCTTGATG GATGACTCAC TGATGCAGGT TGCGCTGCAG
    CTGCTTTGTG TCTACACCGC AAATTTCCCA AATGGTTGCA GTTCCCTCTG TTGGTCAAGT
    TATGGACAGC ATCCTGTTCA AGCCGCACAC CGAGGGGCCC CCGGCAGCTC CCTGATGCTG
    TGTATCCTGA AGATGGCTTC CCAGGTGCCT CCAGAGAACA CGGCTGTTCA GCAGGCAGTG
    TTCATGATTC TTTCAAACCT GGCCTTGTCT CATGACTGTA AAGGGGTAAT TCAGAAAAGT
    AACTTCCTGC AGAACTTTCT GTCTCTGACG TTGCCGAAAG GGGGAAACAA ACATCTGAGT
    AACCTGAGCG TGCTCTGGCT GAAGCTGCTA CTGAACATGT CCTTCGAAGA AGATGGGCAG
    CAGGTGATCC TGCGGCTGGA CGGCTGTCTA GACCTGCTCA CAGAGATGAG CAGGTTCAAG
    CACAAGAGCA GCCTCTCCAT CCCCCTTCTT ATTTTCCATA ACATCTGCTT CAGTCCTGCC
    AACAAGCCCA AGATCCTGGC TAACGAGAAA GTCATTAGTG TGCTCGCTGC CTGTCTGGAA
    AGTGAGAATC AAAACGCTCA GAGGATTGGA GCAGCCGCAC TGTGGGCTCT CATTCACAAT
    TACCAGAAGG CAAAAACAAC TTTGAAAAAC CCATCAATAA AAAGAAGAGT GGAAGAAGCA
    TATTCCTTAG CAAAGAAAAC TTTCTCAGAC TCAGAAGAAA CCCCTCTAAG TGCCTATTAT
    TTGAAATGTC TTGAAAACCT TGTGCAGCAG CTTAACTCTT CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36051
    Clone ID Related Accession (Same CDS sequence) NM_001206207.1
    Accession Version NM_001206207.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6687bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product rotatin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000024.1. On Apr 20, 2011 this sequence version replaced XM_867169.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145432 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGTCCTGA CGGGGCTCAT CCGGAAACTC GGTCACCCGC TGGCCGAGAT CCGGGAGCGC 
    GCGCTCAGGA GTATTCTCTG CAAGCTCGAG CACAGCTTGG TCTGCGGCGC GGACCTGGCT
    TCGCACAGGC TGCTGTTTCT CCACTTGCTG GAATGGTTCA ATTTCCCGTC CGTCCCAGTG
    AAGGACGAGG TCCTGGGTCT GCTGAGCAGA CTGGTTAAGT ATCCTCTAGC AGTGCAGCAT
    TTGGTTGATC TTGGCGCGGT CGAATTCTTA TCTAAACTTC GCCCTAATGT GGAGCCGAAT
    CTACAGGCAG AAATTGATGG CATTCTGGAT GGACTTTTTA GTCTTCCTTC AGAAGTTCCT
    GAAATGTATT CTTCATCTTA CCAGACCAGT CAAACTGATT TGCTGCAACA ACCTGAAATC
    TTAACAGGAT ATTTTCCTCA AGACAAAAGT ACTTTGCAGC AGACAGAAAT ACCTTCACGA
    CCTGTGGCAA ATCCGACCGC AAAGTGTTTG AAGTTTTCTA CTTTCCCCTG GCTCCCCCTG
    ACCGCAGCTG ACAGACACGT TCTCTCGTCC AACGAAAGCT CTTTAAGAAG TAGTAACCAT
    ACTGTAATCT GGAACACCTG TGAACTGTTG AAAGACGTTA TCATGCAAGA TTTTCCTGCT
    GAGATATTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC
    TTTGGAGGAG ACGGGAAGCA TCGTCTGGCA TTACAGTCAG TGTCCTGCCT GCAGCAGCTG
    TGCACGTGTT TAAGAAACAG ACTCAATTTC CACAGAGATC CAAGTTTCTT CTCTAATAAA
    CAAGATGCCG TGTCCCAGAA CTCTTCCCTG TCTTACTGTC CTGATGCCAG GGCGGCTCCG
    CATTCCCAGA ACCCGTCTCC CGGAAGCAGC AGCCCGCGGC CCTCCGTGGT GGGGCGCACG
    GGCCAGCGGC CCCGGGGGGA CGGCCAGGAC TGGGACGCCG TGTCCTCCAG TGGGAGTAGC
    AGCCACGCTC ACGTCAGCTC GCGGGCCTCT GCCCCCTCGC CTCTGGACCT GGCGCCCGTG
    GACCTGCCGG AGCTGGCAGA GGACGCGCTG GAGCTGCAGC TGCAGCAGCT GAGCCTGCCC
    CAGCTCTGCG TCTCCCTCCT GGAGGCCGCC GCGCCCCTCC TGAGGACAGG TAGTAGACAG
    ATGATAATAA GAGTTCTGGA GTTGCTCGCA GAAGTTGTGG TGCTTATTGG TGAAGCGATT
    TCAGCACATC TGTGGGATGA CAGCAGTCTT TTTGCAATAG ATATGAAAGA GAAGCTGCTC
    GTGGTGTTGG CGTCCCTTGG AGACACCGTG TGCTACCATA AAAGCTGTGT CAGTCTGGAG
    CAGCCGGAGG CGCTGGTCGT GCACCACAGG ATGGCCTTTG TCAGCATCTC GCTGTTTGCT
    GTCCGGCTCC TGCAGACGCT GCTCCCTGTG GAAAAGGCAA GCCGCTTTTT ACCGGAGCCT
    ATGTTAGCAG CATTATTTTT TATTTCTTTG GACATGCCTG TTTCTTTGGA ATATCCAAAC
    ATTCACGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT
    AAACGAACTG CAGAGGCTGC TTATTCAATT GAGTGTACCT GTAACTTCCT GTCTGATGTT
    GGGAAGGAGG GAGAAAAGAA TCTCTCAGAG TTAGTGGAGC TGGCAGATCA AGCCTTACGT
    AGCTTTTCCT ACCATCAGCA TTTCCCCCTA ATAAAGGAAA TCATCTCCAT TTGTTCAAAA
    ATCTGGAAAT CCGTGCAGGC CAGTCCTTTA CTACAAGGAG AAAGTCAGAA GGTGTTTCTG
    CGGATGTTGT CTCACCCTTT GCTACAAGTG AAAGTTGAAA CTTACCACTG CTGTCTGGAA
    ATTGTTAAGG AATGTTTAGG CATCCATAAT ATCAGTAAAC CTGTGTCCTC CCTTTGTAAT
    GGAATTCATT TTCTACTTCA CCCAAAAGTT CTGTATGAAA TCTCTGCATT TGGCATTCAA
    GACCCCAAAA ATGAGGTGAG CGCCGCAGCC AAGGCCATCC TGGTGCACCT GCTGCAGGGC
    CGACTGAGGG TGTCGGCGCC GACCTGGAAC AAGTTCATCG AGTCCCTGTG CCCCGTCATC
    CCCGTGCTGC AGGGCTACGC CGACACTGAA GACCCTCTGG GGAACTGCAT CCTCCACCTC
    AGCAGAGAGA GTCCAGAGAC CGATGAAGGG GTGCTCCCCT CTGCCACCCG GCTGAAGTCC
    ATGCTGCGGC TCCTGCTGGT GAAGAAGCCC TCGGTCCGGG TGCTGGCATT AAAGCTCTTA
    GCATCTCACC TCACAAGGGA AGAGGGGTCT GACACCAAGC GCCCTTCAAT AGATGCCAGA
    GTTCTCTCCA GAGTTACTAA TTTATTTATT GTGAAAAAAC CTATTGAACT CAAATTGGAT
    GACCGAAGAG AACTGATGAT TAAGTTGGAG ACTGTTGAAA AGGTGTATGG AATCTTTGTC
    TCAGATGATG TTGATCTTGT TTTGAGAAAG TCAGCCGCAG AGCAGTTAGT GGTGATTATG
    CAAGATATTA AAATGCATGC TGTGGTGAAA AAAGTGCACC TAGTTGACAA GATAATTGAG
    TATTTAAGTG AATGTGTGGG TCAGGATGGT GAGGTGGTGG AGTGTTTGGT TCAGCCGAGC
    CTCATGCTCC TGAGGAAGGT CCTCTGTGCC GACCCCGTCC TGCGAGAGTC TCTGTCCCAG
    AAGACTTCTG TCCTGACCCT GCTCTTCAGA GTTTCCTTGA TATTTCATGA AGATTGTACC
    ATTGTGACTG AAGTTGGAGC ATTGTTTTGT CTTCTGTTAT TTGATGAAGT ATCAAGAATG
    GATATGTGGT CTGATAGTCC TTCCTGCACA CCATCTTCAC CATCAGTCTT CAGTCTGCCT
    GTTTCAGTTT CTAGAAGGTA TCATCTACCA GTGCATGTAG TTGGCCACCA TGCCGTGAGT
    CCTTACTCCA TGGTTTTGCC GTTATCTGCT GACTCTTTGG CCTTGAAGCC GGTATCAGAT
    ATGCTGAGGA TGGCTTGGAA CCTGTCGTGG TATCACGGGA GTGACAATGT GTTGAAGCAA
    GTGAACTCCG AAGCTGAAAT CCAGGAATTT TTAGATACAC TGAAGTTATC CACCGAGGAC
    ACCCTTACTC TGAAGATGAC CCACACACCT AGTGGACTCA AGGACTGTCT CTGCTCCATT
    GTTCAGGCCG CAGGCCACAG GGAAGTGAGG GCTGCCCTCA CAAGGATGAG TTTTTATCTC
    CTAAATGACA GACTGTCTTT CAAGGGCGGC ACTGGCCCCT GTGGGGTTAC CCTGAAGTCC
    TTGTCTTGGC ACACCACACT AAACAGGTTT TTACAAGTTC TTCCTGCTTG CACTGAAGAT
    GAGAAGCTGT TAATAGATAT CATACATTTT CTCAGTAAGT TATTGAAGGA ACAAAGGAAA
    AATGTACCCG TAGAACTTCT AAACTGGCTT CTAGAAGTAC TTCTTAGACA AAGTCCAAAT
    CCACTGTTGG ACCTGCTGGT TCTGACGGAG TCCCAGACAC GAGAGGAAAC AGATGATGTC
    CGCACTGCGG TCAGGCAGCA GCTCCAGAAA GAGCTGATTG CTCTCTTTAG TACCTTGCTG
    CTCAGTTTTG AGGCTGTTAC TGACAGGAAA TGCTTGGAGC TTTTTTACGT TTTCCAAACG
    CAGCTGGCCC TGAAACTACT CCAGTGTCTG AGAGTCACGG ACGCTCCTCA CTTCTATGGC
    CTACCGTCCC TTGAGAGGAC CTTGCGGGGG ATGGCCCACC TCACGGCGTG TCCAGGGTGG
    AGCACACACT CTCCTCTGAC AAAGCCACTC GACATTTGTG GGAAATACTT GTCAGGTCTC
    CTCGAAGTCA TCACATCCTT CTACGTGGAG CGAGGAGGAA ATGCGATGTC CTTCATGGGA
    AAAGGTGTCA CAAAGAGCAC CGTTCTCTGC CTGCTCCACT TGTCGCATGA GATGATGGCC
    CAGGCCCAGA GCTCGGGGTG GATGTCACTT TGGTTCTTGC CTTTGGGGAG TCAGAGTGAA
    GACCATGCTC CCACTCAGCA GGGATTGGCT TGGCTGATTC CGTTGTGGGT TGACCGAGAC
    CCAGAGGTGA GGTTCACTTC ATTGGGATTA GGATCAGCAC TAACCACCCT TGAAACTGGC
    TGTGTGGCCT TGGCAAACAG TTGTCAAAAC ATTTCTGGTG GGCTCTGGGG AACGGTGGTG
    AACATTCTTC TGGACCAGTC GGAATGCAGC ATGGTGCGCC GGGAGGCTGC ATTTATTTTT
    CAGAATCTCC TTGTAATTCC AATGCCTTCA GTAATTATAA AGGATTATAC TTGGCAGGGC
    CCCTGTGTCC ATGATGAGGA CTCTGGGCTG TCTCTCATAG GAAAACCTGC CCTCCAGGCT
    CTTTTATACC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAACA TTGTTACCTG
    GGACGGTATG CATTTGATTT CAGTTTTTCA GCTCTTAATG GAGCTCCTGA AGGCAATGAT
    TTAAATGGTT TAGATGACTC GTTCAAGTTC TGGAGGGCTC CATCTCGAAT GTCCAGTCAA
    GATCGAGATC CAAGTTCTCT CTCTACCTCG GAAACAATGG TGGCAGCTTC TTCGGGCAGT
    GCTGAATTTC AGTCCCTCGT GCTCTCCATG GTGCCTCACC CTGAAGTCAC ACACGACCAG
    CTGGTGGCCC AAGGTCAGCG TGAAGCTGCA TCTCCACGGC TTCCTCCTGA TTCACCCCTC
    TCTGCTCCTC TGCCCAGGCA GTGTGTGTTT GTCACCCCGC CCCTCCTCTC CGCGATGTGC
    AGCCTCCTAG ACAGCCTCTT GGTGGCCACT CCAGGCGACA CTGCCGGCGC TTTCCGACGA
    ACCCGTCTCA TTGAGCTTCT CTGTAGCATC GCAGTTGCCA CCCTCCTAGA GACAAGTGTC
    CAGGAGCTCC GGGCCTCGCT GCCTACATCT CTTCCTGCTG AACACACTCA GGCTCAGGTG
    TCCTTTCTCC TGGAATACTT GTCCTCCTTA TCCCGGCTCC TGCAGTCATG TTTATTGGTG
    GATCCCGACC TTGTGATTCA GGATGAGCTT CTGAAACCTC TTATCCCAAA CATCATCCGA
    GTTCTCACCC TATGCACCAA AGATATATTA GATGTAGAGT TAAGGTCAGC TTTTTATCAG
    ACATGGACTC ATCTGTTCAA CCTTCTGGCC GCACTGCTGA GGCGAGCTGG CCCTGCCTCT
    TTCCCATATG TTACCCTGGG TCTGGCCCGG TCTTGGGCAG CGGTGATCGA TACATTCTGC
    AGGTGTGCGC GTTTGTCTAC CACAAGCCCT GCTCTGTACA CCGCCAGCCT GCAGTTCCTT
    TCTGTTCTCT TGACCGAAGA AGCAAAAAGG CGTCTCCAGG ATGAGGAGAA AACAAGCTTA
    TGCCACAGTC CAACAGTGGC TTCACTTCTT GACAAAACCC AGGAAAGTCA GAACTCACTA
    GAACGACTCA CTGAAGTCAT TCTTCAGTGC TATGAGGGGA AAACCTCCAA AGATGTCCTG
    AAGAGGGTCG CTGCGAACGC ACTGATGTCG CTTCTGGCCG TCAGCAGAAG AGCACAGAGA
    CATGCGCTGA AGGCCCATCT TGTAGACAGC TGCGTGGAGC AGATGAAGCA CATTCATGCA
    CAGCTGAACC TGGGTTCTCT GAAGCCGGGA AGGGCAGTGC TGAGGAAAAA GGAGGATGGT
    TTTATTAAAG AATTAAGCAT TGCCATGCAG CTCCTAAGAA ACTGTCTTTA TCAGAATGAA
    GAATGTAAAG GGGCGGCTCT GGACGCTCAC CTGGCCCCTG TCCTGCACGC TCTCTGGCCT
    TGGCTCTTGA TGGATGACTC ACTGATGCAG GTTGCGCTGC AGCTGCTTTG TGTCTACACC
    GCAAATTTCC CAAATGGTTG CAGTTCCCTC TGTTGGTCAA GTTATGGACA GCATCCTGTT
    CAAGCCGCAC ACCGAGGGGC CCCCGGCAGC TCCCTGATGC TGTGTATCCT GAAGATGGCT
    TCCCAGGTGC CTCCAGAGAA CACGGCTGTT CAGCAGGCAG TGTTCATGAT TCTTTCAAAC
    CTGGCCTTGT CTCATGACTG TAAAGGGGTA ATTCAGAAAA GTAACTTCCT GCAGAACTTT
    CTGTCTCTGA CGTTGCCGAA AGGGGGAAAC AAACATCTGA GTAACCTGAG CGTGCTCTGG
    CTGAAGCTGC TACTGAACAT GTCCTTCGAA GAAGATGGGC AGCAGGTGAT CCTGCGGCTG
    GACGGCTGTC TAGACCTGCT CACAGAGATG AGCAGGTTCA AGCACAAGAG CAGCCTCTCC
    ATCCCCCTTC TTATTTTCCA TAACATCTGC TTCAGTCCTG CCAACAAGCC CAAGATCCTG
    GCTAACGAGA AAGTCATTAG TGTGCTCGCT GCCTGTCTGG AAAGTGAGAA TCAAAACGCT
    CAGAGGATTG GAGCAGCCGC ACTGTGGGCT CTCATTCACA ATTACCAGAA GGCAAAAACA
    ACTTTGAAAA ACCCATCAAT AAAAAGAAGA GTGGAAGAAG CATATTCCTT AGCAAAGAAA
    ACTTTCTCAG ACTCAGAAGA AACCCCTCTA AGTGCCTATT ATTTGAAATG TCTTGAAAAC
    CTTGTGCAGC AGCTTAACTC TTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193136.1
    CDS9..6695
    Translation

    Target ORF information:

    RefSeq Version NM_001206207.1
    Organism Bos taurus(cattle)
    Definition Bos taurus rotatin (RTTN), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206207.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGTCCTGA CGGGGCTCAT CCGGAAACTC GGTCACCCGC TGGCCGAGAT CCGGGAGCGC 
    GCGCTCAGGA GTATTCTCTG CAAGCTCGAG CACAGCTTGG TCTGCGGCGC GGACCTGGCT
    TCGCACAGGC TGCTGTTTCT CCACTTGCTG GAATGGTTCA ATTTCCCGTC CGTCCCAGTG
    AAGGACGAGG TCCTGGGTCT GCTGAGCAGA CTGGTTAAGT ATCCTCTAGC AGTGCAGCAT
    TTGGTTGATC TTGGCGCGGT CGAATTCTTA TCTAAACTTC GCCCTAATGT GGAGCCGAAT
    CTACAGGCAG AAATTGATGG CATTCTGGAT GGACTTTTTA GTCTTCCTTC AGAAGTTCCT
    GAAATGTATT CTTCATCTTA CCAGACCAGT CAAACTGATT TGCTGCAACA ACCTGAAATC
    TTAACAGGAT ATTTTCCTCA AGACAAAAGT ACTTTGCAGC AGACAGAAAT ACCTTCACGA
    CCTGTGGCAA ATCCGACCGC AAAGTGTTTG AAGTTTTCTA CTTTCCCCTG GCTCCCCCTG
    ACCGCAGCTG ACAGACACGT TCTCTCGTCC AACGAAAGCT CTTTAAGAAG TAGTAACCAT
    ACTGTAATCT GGAACACCTG TGAACTGTTG AAAGACGTTA TCATGCAAGA TTTTCCTGCT
    GAGATATTCC TTCAAAGGCC AAAAATTGTT CAGAGCCTTT TATCTCTGTT GAAACTGGCC
    TTTGGAGGAG ACGGGAAGCA TCGTCTGGCA TTACAGTCAG TGTCCTGCCT GCAGCAGCTG
    TGCACGTGTT TAAGAAACAG ACTCAATTTC CACAGAGATC CAAGTTTCTT CTCTAATAAA
    CAAGATGCCG TGTCCCAGAA CTCTTCCCTG TCTTACTGTC CTGATGCCAG GGCGGCTCCG
    CATTCCCAGA ACCCGTCTCC CGGAAGCAGC AGCCCGCGGC CCTCCGTGGT GGGGCGCACG
    GGCCAGCGGC CCCGGGGGGA CGGCCAGGAC TGGGACGCCG TGTCCTCCAG TGGGAGTAGC
    AGCCACGCTC ACGTCAGCTC GCGGGCCTCT GCCCCCTCGC CTCTGGACCT GGCGCCCGTG
    GACCTGCCGG AGCTGGCAGA GGACGCGCTG GAGCTGCAGC TGCAGCAGCT GAGCCTGCCC
    CAGCTCTGCG TCTCCCTCCT GGAGGCCGCC GCGCCCCTCC TGAGGACAGG TAGTAGACAG
    ATGATAATAA GAGTTCTGGA GTTGCTCGCA GAAGTTGTGG TGCTTATTGG TGAAGCGATT
    TCAGCACATC TGTGGGATGA CAGCAGTCTT TTTGCAATAG ATATGAAAGA GAAGCTGCTC
    GTGGTGTTGG CGTCCCTTGG AGACACCGTG TGCTACCATA AAAGCTGTGT CAGTCTGGAG
    CAGCCGGAGG CGCTGGTCGT GCACCACAGG ATGGCCTTTG TCAGCATCTC GCTGTTTGCT
    GTCCGGCTCC TGCAGACGCT GCTCCCTGTG GAAAAGGCAA GCCGCTTTTT ACCGGAGCCT
    ATGTTAGCAG CATTATTTTT TATTTCTTTG GACATGCCTG TTTCTTTGGA ATATCCAAAC
    ATTCACGAAG CTGTTGTGGC CTATTTGGAA CAGCTGAATT CTGAAAACTA CAGTATTTAT
    AAACGAACTG CAGAGGCTGC TTATTCAATT GAGTGTACCT GTAACTTCCT GTCTGATGTT
    GGGAAGGAGG GAGAAAAGAA TCTCTCAGAG TTAGTGGAGC TGGCAGATCA AGCCTTACGT
    AGCTTTTCCT ACCATCAGCA TTTCCCCCTA ATAAAGGAAA TCATCTCCAT TTGTTCAAAA
    ATCTGGAAAT CCGTGCAGGC CAGTCCTTTA CTACAAGGAG AAAGTCAGAA GGTGTTTCTG
    CGGATGTTGT CTCACCCTTT GCTACAAGTG AAAGTTGAAA CTTACCACTG CTGTCTGGAA
    ATTGTTAAGG AATGTTTAGG CATCCATAAT ATCAGTAAAC CTGTGTCCTC CCTTTGTAAT
    GGAATTCATT TTCTACTTCA CCCAAAAGTT CTGTATGAAA TCTCTGCATT TGGCATTCAA
    GACCCCAAAA ATGAGGTGAG CGCCGCAGCC AAGGCCATCC TGGTGCACCT GCTGCAGGGC
    CGACTGAGGG TGTCGGCGCC GACCTGGAAC AAGTTCATCG AGTCCCTGTG CCCCGTCATC
    CCCGTGCTGC AGGGCTACGC CGACACTGAA GACCCTCTGG GGAACTGCAT CCTCCACCTC
    AGCAGAGAGA GTCCAGAGAC CGATGAAGGG GTGCTCCCCT CTGCCACCCG GCTGAAGTCC
    ATGCTGCGGC TCCTGCTGGT GAAGAAGCCC TCGGTCCGGG TGCTGGCATT AAAGCTCTTA
    GCATCTCACC TCACAAGGGA AGAGGGGTCT GACACCAAGC GCCCTTCAAT AGATGCCAGA
    GTTCTCTCCA GAGTTACTAA TTTATTTATT GTGAAAAAAC CTATTGAACT CAAATTGGAT
    GACCGAAGAG AACTGATGAT TAAGTTGGAG ACTGTTGAAA AGGTGTATGG AATCTTTGTC
    TCAGATGATG TTGATCTTGT TTTGAGAAAG TCAGCCGCAG AGCAGTTAGT GGTGATTATG
    CAAGATATTA AAATGCATGC TGTGGTGAAA AAAGTGCACC TAGTTGACAA GATAATTGAG
    TATTTAAGTG AATGTGTGGG TCAGGATGGT GAGGTGGTGG AGTGTTTGGT TCAGCCGAGC
    CTCATGCTCC TGAGGAAGGT CCTCTGTGCC GACCCCGTCC TGCGAGAGTC TCTGTCCCAG
    AAGACTTCTG TCCTGACCCT GCTCTTCAGA GTTTCCTTGA TATTTCATGA AGATTGTACC
    ATTGTGACTG AAGTTGGAGC ATTGTTTTGT CTTCTGTTAT TTGATGAAGT ATCAAGAATG
    GATATGTGGT CTGATAGTCC TTCCTGCACA CCATCTTCAC CATCAGTCTT CAGTCTGCCT
    GTTTCAGTTT CTAGAAGGTA TCATCTACCA GTGCATGTAG TTGGCCACCA TGCCGTGAGT
    CCTTACTCCA TGGTTTTGCC GTTATCTGCT GACTCTTTGG CCTTGAAGCC GGTATCAGAT
    ATGCTGAGGA TGGCTTGGAA CCTGTCGTGG TATCACGGGA GTGACAATGT GTTGAAGCAA
    GTGAACTCCG AAGCTGAAAT CCAGGAATTT TTAGATACAC TGAAGTTATC CACCGAGGAC
    ACCCTTACTC TGAAGATGAC CCACACACCT AGTGGACTCA AGGACTGTCT CTGCTCCATT
    GTTCAGGCCG CAGGCCACAG GGAAGTGAGG GCTGCCCTCA CAAGGATGAG TTTTTATCTC
    CTAAATGACA GACTGTCTTT CAAGGGCGGC ACTGGCCCCT GTGGGGTTAC CCTGAAGTCC
    TTGTCTTGGC ACACCACACT AAACAGGTTT TTACAAGTTC TTCCTGCTTG CACTGAAGAT
    GAGAAGCTGT TAATAGATAT CATACATTTT CTCAGTAAGT TATTGAAGGA ACAAAGGAAA
    AATGTACCCG TAGAACTTCT AAACTGGCTT CTAGAAGTAC TTCTTAGACA AAGTCCAAAT
    CCACTGTTGG ACCTGCTGGT TCTGACGGAG TCCCAGACAC GAGAGGAAAC AGATGATGTC
    CGCACTGCGG TCAGGCAGCA GCTCCAGAAA GAGCTGATTG CTCTCTTTAG TACCTTGCTG
    CTCAGTTTTG AGGCTGTTAC TGACAGGAAA TGCTTGGAGC TTTTTTACGT TTTCCAAACG
    CAGCTGGCCC TGAAACTACT CCAGTGTCTG AGAGTCACGG ACGCTCCTCA CTTCTATGGC
    CTACCGTCCC TTGAGAGGAC CTTGCGGGGG ATGGCCCACC TCACGGCGTG TCCAGGGTGG
    AGCACACACT CTCCTCTGAC AAAGCCACTC GACATTTGTG GGAAATACTT GTCAGGTCTC
    CTCGAAGTCA TCACATCCTT CTACGTGGAG CGAGGAGGAA ATGCGATGTC CTTCATGGGA
    AAAGGTGTCA CAAAGAGCAC CGTTCTCTGC CTGCTCCACT TGTCGCATGA GATGATGGCC
    CAGGCCCAGA GCTCGGGGTG GATGTCACTT TGGTTCTTGC CTTTGGGGAG TCAGAGTGAA
    GACCATGCTC CCACTCAGCA GGGATTGGCT TGGCTGATTC CGTTGTGGGT TGACCGAGAC
    CCAGAGGTGA GGTTCACTTC ATTGGGATTA GGATCAGCAC TAACCACCCT TGAAACTGGC
    TGTGTGGCCT TGGCAAACAG TTGTCAAAAC ATTTCTGGTG GGCTCTGGGG AACGGTGGTG
    AACATTCTTC TGGACCAGTC GGAATGCAGC ATGGTGCGCC GGGAGGCTGC ATTTATTTTT
    CAGAATCTCC TTGTAATTCC AATGCCTTCA GTAATTATAA AGGATTATAC TTGGCAGGGC
    CCCTGTGTCC ATGATGAGGA CTCTGGGCTG TCTCTCATAG GAAAACCTGC CCTCCAGGCT
    CTTTTATACC ACTGCCATTT TTATGAACAT TTGAATCAGA TGGTAAAACA TTGTTACCTG
    GGACGGTATG CATTTGATTT CAGTTTTTCA GCTCTTAATG GAGCTCCTGA AGGCAATGAT
    TTAAATGGTT TAGATGACTC GTTCAAGTTC TGGAGGGCTC CATCTCGAAT GTCCAGTCAA
    GATCGAGATC CAAGTTCTCT CTCTACCTCG GAAACAATGG TGGCAGCTTC TTCGGGCAGT
    GCTGAATTTC AGTCCCTCGT GCTCTCCATG GTGCCTCACC CTGAAGTCAC ACACGACCAG
    CTGGTGGCCC AAGGTCAGCG TGAAGCTGCA TCTCCACGGC TTCCTCCTGA TTCACCCCTC
    TCTGCTCCTC TGCCCAGGCA GTGTGTGTTT GTCACCCCGC CCCTCCTCTC CGCGATGTGC
    AGCCTCCTAG ACAGCCTCTT GGTGGCCACT CCAGGCGACA CTGCCGGCGC TTTCCGACGA
    ACCCGTCTCA TTGAGCTTCT CTGTAGCATC GCAGTTGCCA CCCTCCTAGA GACAAGTGTC
    CAGGAGCTCC GGGCCTCGCT GCCTACATCT CTTCCTGCTG AACACACTCA GGCTCAGGTG
    TCCTTTCTCC TGGAATACTT GTCCTCCTTA TCCCGGCTCC TGCAGTCATG TTTATTGGTG
    GATCCCGACC TTGTGATTCA GGATGAGCTT CTGAAACCTC TTATCCCAAA CATCATCCGA
    GTTCTCACCC TATGCACCAA AGATATATTA GATGTAGAGT TAAGGTCAGC TTTTTATCAG
    ACATGGACTC ATCTGTTCAA CCTTCTGGCC GCACTGCTGA GGCGAGCTGG CCCTGCCTCT
    TTCCCATATG TTACCCTGGG TCTGGCCCGG TCTTGGGCAG CGGTGATCGA TACATTCTGC
    AGGTGTGCGC GTTTGTCTAC CACAAGCCCT GCTCTGTACA CCGCCAGCCT GCAGTTCCTT
    TCTGTTCTCT TGACCGAAGA AGCAAAAAGG CGTCTCCAGG ATGAGGAGAA AACAAGCTTA
    TGCCACAGTC CAACAGTGGC TTCACTTCTT GACAAAACCC AGGAAAGTCA GAACTCACTA
    GAACGACTCA CTGAAGTCAT TCTTCAGTGC TATGAGGGGA AAACCTCCAA AGATGTCCTG
    AAGAGGGTCG CTGCGAACGC ACTGATGTCG CTTCTGGCCG TCAGCAGAAG AGCACAGAGA
    CATGCGCTGA AGGCCCATCT TGTAGACAGC TGCGTGGAGC AGATGAAGCA CATTCATGCA
    CAGCTGAACC TGGGTTCTCT GAAGCCGGGA AGGGCAGTGC TGAGGAAAAA GGAGGATGGT
    TTTATTAAAG AATTAAGCAT TGCCATGCAG CTCCTAAGAA ACTGTCTTTA TCAGAATGAA
    GAATGTAAAG GGGCGGCTCT GGACGCTCAC CTGGCCCCTG TCCTGCACGC TCTCTGGCCT
    TGGCTCTTGA TGGATGACTC ACTGATGCAG GTTGCGCTGC AGCTGCTTTG TGTCTACACC
    GCAAATTTCC CAAATGGTTG CAGTTCCCTC TGTTGGTCAA GTTATGGACA GCATCCTGTT
    CAAGCCGCAC ACCGAGGGGC CCCCGGCAGC TCCCTGATGC TGTGTATCCT GAAGATGGCT
    TCCCAGGTGC CTCCAGAGAA CACGGCTGTT CAGCAGGCAG TGTTCATGAT TCTTTCAAAC
    CTGGCCTTGT CTCATGACTG TAAAGGGGTA ATTCAGAAAA GTAACTTCCT GCAGAACTTT
    CTGTCTCTGA CGTTGCCGAA AGGGGGAAAC AAACATCTGA GTAACCTGAG CGTGCTCTGG
    CTGAAGCTGC TACTGAACAT GTCCTTCGAA GAAGATGGGC AGCAGGTGAT CCTGCGGCTG
    GACGGCTGTC TAGACCTGCT CACAGAGATG AGCAGGTTCA AGCACAAGAG CAGCCTCTCC
    ATCCCCCTTC TTATTTTCCA TAACATCTGC TTCAGTCCTG CCAACAAGCC CAAGATCCTG
    GCTAACGAGA AAGTCATTAG TGTGCTCGCT GCCTGTCTGG AAAGTGAGAA TCAAAACGCT
    CAGAGGATTG GAGCAGCCGC ACTGTGGGCT CTCATTCACA ATTACCAGAA GGCAAAAACA
    ACTTTGAAAA ACCCATCAAT AAAAAGAAGA GTGGAAGAAG CATATTCCTT AGCAAAGAAA
    ACTTTCTCAG ACTCAGAAGA AACCCCTCTA AGTGCCTATT ATTTGAAATG TCTTGAAAAC
    CTTGTGCAGC AGCTTAACTC TTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32440
    Clone ID Related Accession (Same CDS sequence) XM_005224039.3 , XM_005224039.4
    Accession Version XM_005224039.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rotatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104566.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224039.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGTCCTGA CGGGGCTCAT CCGGAAACTC GGTCACCCGC TGGCCGAGAT CCGGGAGCGC 
    GCGCTCAGGA GTATTCTCTG CAAGCTCGAG CACAGCTTGG TCTGCGGCGC GGACCTGGCT
    TCGCACAGGC TGCTGTTTCT CCACTTGCTG GAATGGTTCA ATTTCCCGTC CGTCCCAGTG
    AAGGACGAGG TCCTGGGTCT GCTGAGCAGA CTGGTTAAGT ATCCTCTAGC AGTGCAGCAT
    TTGGTTGATC TTGGCGCGGT CGAATTCTTA TCTAAACTTC GCCCTAATGT GGAGCCGAAT
    CTACAGGCAG AAATTGATGG CATTCTGGAT GGACTTTTTA GTCTTCCTTC AGAAGTTCCT
    GAAATGTATT CTTCATCTTA CCAGACCAGT CAAACTGATT TGCTGCAACA ACCTGAAATC
    TTAACAGGAT ATTTTCCTCA AGACAAAAGT ACTTTGCAGC AGACAGAAAT ACCTTCACGA
    CCTGTGGCAG CAAATCCGAC CGCAAAGTGT TTGAAGTTTT CTACTTTCCC CTGGCTCCCC
    CTGACCGCAG CTGACAGACA CGTTCTCTCG TCCAACGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTGTAA TCTGGAACAC CTGTGAACTG TTGAAAGACG TTATCATGCA AGATTTTCCT
    GCTGAGATAT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGAG GAGACGGGAA GCATCGTCTG GCATTACAGT CAGTGTCCTG CCTGCAGCAG
    CTGTGCACGT GTTTAAGAAA CAGACTCAAT TTCCACAGAG ATCCAAGTTT CTTCTCTAAT
    AAACAAGATG CCGTGTCCCA GAACTCTTCC CTGTCTTACT GTCCTGATGC CAGGGCGGCT
    CCGCATTCCC AGAACCCGTC TCCCGGAAGC AGCAGCCCGC GGCCCTCCGT GGTGGGGCGC
    ACGGGCCAGC GGCCCCGGGG GGACGGCCAG GACTGGGACG CCGTGTCCTC CAGTGGGAGT
    AGCAGCCACG CTCACGTCAG CTCGCGGGCC TCTGCCCCCT CGCCTCTGGA CCTGGCGCCC
    GTGGACCTGC CGGAGCTGGC AGAGGACGCG CTGGAGCTGC AGCTGCAGCA GCTGAGCCTG
    CCCCAGCTCT GCGTCTCCCT CCTGGAGGCC GCCGCGCCCC TCCTGAGGAC AGGTAGTAGA
    CAGATGATAA TAAGAGTTCT GGAGTTGCTC GCAGAAGTTG TGGTGCTTAT TGGTGAAGCG
    ATTTCAGCAC ATCTGTGGGA TGACAGCAGT CTTTTTGCAA TAGATATGAA AGAGAAGCTG
    CTCGTGGTGT TGGCGTCCCT TGGAGACACC GTGTGCTACC ATAAAAGCTG TGTCAGTCTG
    GAGCAGCCGG AGGCGCTGGT CGTGCACCAC AGGATGGCCT TTGTCAGCAT CTCGCTGTTT
    GCTGTCCGGC TCCTGCAGAC GCTGCTCCCT GTGGAAAAGG CAAGCCGCTT TTTACCGGAG
    CCTATGTTAG CAGCATTATT TTTTATTTCT TTGGACATGC CTGTTTCTTT GGAATATCCA
    AACATTCACG AAGCTGTTGT GGCCTATTTG GAACAGCTGA ATTCTGAAAA CTACAGTATT
    TATAAACGAA CTGCAGAGGC TGCTTATTCA ATTGAGTGTA CCTGTAACTT CCTGTCTGAT
    GTTGGGAAGG AGGGAGAAAA GAATCTCTCA GAGTTAGTGG AGCTGGCAGA TCAAGCCTTA
    CGTAGCTTTT CCTACCATCA GCATTTCCCC CTAATAAAGG AAATCATCTC CATTTGTTCA
    AAAATCTGGA AATCCGTGCA GGCCAGTCCT TTACTACAAG GAGAAAGTCA GAAGGTGTTT
    CTGCGGATGT TGTCTCACCC TTTGCTACAA GTGAAAGTTG AAACTTACCA CTGCTGTCTG
    GAAATTGTTA AGGAATGTTT AGGCATCCAT AATATCAGTA AACCTGTGTC CTCCCTTTGT
    AATGGAATTC ATTTTCTACT TCACCCAAAA GTTCTGTATG AAATCTCTGC ATTTGGCATT
    CAAGACCCCA AAAATGAGGT GAGCGCCGCA GCCAAGGCCA TCCTGGTGCA CCTGCTGCAG
    GGCCGACTGA GGGTGTCGGC GCCGACCTGG AACAAGTTCA TCGAGTCCCT GTGCCCCGTC
    ATCCCCGTGC TGCAGGGCTA CGCCGACACT GAAGACCCTC TGGGGAACTG CATCCTCCAC
    CTCAGCAGAG AGAGTCCAGA GACCGATGAA GGGGTGCTCC CCTCTGCCAC CCGGCTGAAG
    TCCATGCTGC GGCTCCTGCT GGTGAAGAAG CCCTCGGTCC GGGTGCTGGC ATTAAAGCTC
    TTAGCATCTC ACCTCACAAG GGAAGAGGGG TCTGACACCA AGCGCCCTTC AATAGATGCC
    AGAGTTCTCT CCAGAGTTAC TAATTTATTT ATTGTGAAAA AACCTATTGA ACTCAAATTG
    GATGACCGAA GAGAACTGAT GATTAAGTTG GAGACTGTTG AAAAGGTGTA TGGAATCTTT
    GTCTCAGATG ATGTTGATCT TGTTTTGAGA AAGTCAGCCG CAGAGCAGTT AGTGGTGATT
    ATGCAAGATA TTAAAATGCA TGCTGTGGTG AAAAAAGTGC ACCTAGTTGA CAAGATAATT
    GAGTATTTAA GTGAATGTGT GGGTCAGGAT GGTGAGGTGG TGGAGTGTTT GGTTCAGCCG
    AGCCTCATGC TCCTGAGGAA GGTCCTCTGT GCCGACCCCG TCCTGCGAGA GTCTCTGTCC
    CAGAAGACTT CTGTCCTGAC CCTGCTCTTC AGAGTTTCCT TGATATTTCA TGAAGATTGT
    ACCATTGTGA CTGAAGTTGG AGCATTGTTT TGTCTTCTGT TATTTGATGA AGTATCAAGA
    ATGGATATGT GGTCTGATAG TCCTTCCTGC ACACCATCTT CACCATCAGT CTTCAGTCTG
    CCTGTTTCAG TTTCTAGAAG GTATCATCTA CCAGTGCATG TAGTTGGCCA CCATGCCGTG
    AGTCCTTACT CCATGGTTTT GCCGTTATCT GCTGACTCTT TGGCCTTGAA GCCGGTATCA
    GATATGCTGA GGATGGCTTG GAACCTGTCG TGGTATCACG GGAGTGACAA TGTGTTGAAG
    CAAGTGAACT CCGAAGCTGA AATCCAGGAA TTTTTAGATA CACTGAAGTT ATCCACCGAG
    GACACCCTTA CTCTGAAGAT GACCCACACA CCTAGTGGAC TCAAGGACTG TCTCTGCTCC
    ATTGTTCAGG CCGCAGGCCA CAGGGAAGTG AGGGCTGCCC TCACAAGGAT GAGTTTTTAT
    CTCCTAAATG ACAGACTGTC TTTCAAGGGC GGCACTGGCC CCTGTGGGGT TACCCTGAAG
    TCCTTGTCTT GGCACACCAC ACTAAACAGG TTTTTACAAG TTCTTCCTGC TTGCACTGAA
    GATGAGAAGC TGTTAATAGA TATCATACAT TTTCTCAGTA AGTTATTGAA GGAACAAAGG
    AAAAATGTAC CCGTAGAACT TCTAAACTGG CTTCTAGAAG TACTTCTTAG ACAAAGTCCA
    AATCCACTGT TGGACCTGCT GGTTCTGACG GAGTCCCAGA CACGAGAGGA AACAGATGAT
    GTCCGCACTG CGGTCAGGCA GCAGCTCCAG AAAGAGCTGA TTGCTCTCTT TAGTACCTTG
    CTGCTCAGTT TTGAGGCTGT TACTGACAGG AAATGCTTGG AGCTTTTTTA CGTTTTCCAA
    ACGCAGCTGG CCCTGAAACT ACTCCAGTGT CTGAGAGTCA CGGACGCTCC TCACTTCTAT
    GGCCTACCGT CCCTTGAGAG GACCTTGCGG GGGATGGCCC ACCTCACGGC GTGTCCAGGG
    TGGAGCACAC ACTCTCCTCT GACAAAGCCA CTCGACATTT GTGGGAAATA CTTGTCAGGT
    CTCCTCGAAG TCATCACATC CTTCTACGTG GAGCGAGGAG GAAATGCGAT GTCCTTCATG
    GGAAAAGGTG TCACAAAGAG CACCGTTCTC TGCCTGCTCC ACTTGTCGCA TGAGATGATG
    GCCCAGGCCC AGAGCTCGGG GTGGATGTCA CTTTGGTTCT TGCCTTTGGG GAGTCAGAGT
    GAAGACCATG CTCCCACTCA GCAGGGATTG GCTTGGCTGA TTCCGTTGTG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTAACCAC CCTTGAAACT
    GGCTGTGTGG CCTTGGCAAA CAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGAACGGTG
    GTGAACATTC TTCTGGACCA GTCGGAATGC AGCATGGTGC GCCGGGAGGC TGCATTTATT
    TTTCAGAATC TCCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGCCCCTGTG TCCATGATGA GGACTCTGGG CTGTCTCTCA TAGGAAAACC TGCCCTCCAG
    GCTCTTTTAT ACCACTGCCA TTTTTATGAA CATTTGAATC AGATGGTAAA ACATTGTTAC
    CTGGGACGGT ATGCATTTGA TTTCAGTTTT TCAGCTCTTA ATGGAGCTCC TGAAGGCAAT
    GATTTAAATG GTTTAGATGA CTCGTTCAAG TTCTGGAGGG CTCCATCTCG AATGTCCAGT
    CAAGATCGAG ATCCAAGTTC TCTCTCTACC TCGGAAACAA TGGTGGCAGC TTCTTCGGGC
    AGTGCTGAAT TTCAGTCCCT CGTGCTCTCC ATGGTGCCTC ACCCTGAAGT CACACACGAC
    CAGCTGGTGG CCCAAGGTCA GCGTGAAGCT GCATCTCCAC GGCTTCCTCC TGATTCACCC
    CTCTCTGCTC CTCTGCCCAG GCAGTGTGTG TTTGTCACCC CGCCCCTCCT CTCCGCGATG
    TGCAGCCTCC TAGACAGCCT CTTGGTGGCC ACTCCAGGCG ACACTGCCGG CGCTTTCCGA
    CGAACCCGTC TCATTGAGCT TCTCTGTAGC ATCGCAGTTG CCACCCTCCT AGAGACAAGT
    GTCCAGGAGC TCCGGGCCTC GCTGCCTACA TCTCTTCCTG CTGAACACAC TCAGGCTCAG
    GTGTCCTTTC TCCTGGAATA CTTGTCCTCC TTATCCCGGC TCCTGCAGTC ATGTTTATTG
    GTGGATCCCG ACCTTGTGAT TCAGGATGAG CTTCTGAAAC CTCTTATCCC AAACATCATC
    CGAGTTCTCA CCCTATGCAC CAAAGATATA TTAGATGTAG AGTTAAGGTC AGCTTTTTAT
    CAGACATGGA CTCATCTGTT CAACCTTCTG GCCGCACTGC TGAGGCGAGC TGGCCCTGCC
    TCTTTCCCAT ATGTTACCCT GGGTCTGGCC CGGTCTTGGG CAGCGGTGAT CGATACATTC
    TGCAGGTGTG CGCGTTTGTC TACCACAAGC CCTGCTCTGT ACACCGCCAG CCTGCAGTTC
    CTTTCTGTTC TCTTGACCGA AGAAGCAAAA AGGCGTCTCC AGGATGAGGA GAAAACAAGC
    TTATGCCACA GTCCAACAGT GGCTTCACTT CTTGACAAAA CCCAGGAAAG TCAGAACTCA
    CTAGAACGAC TCACTGAAGT CATTCTTCAG TGCTATGAGG GGAAAACCTC CAAAGATGTC
    CTGAAGAGGG TCGCTGCGAA CGCACTGATG TCGCTTCTGG CCGTCAGCAG AAGAGCACAG
    AGACATGCGC TGAAGGCCCA TCTTGTAGAC AGCTGCGTGG AGCAGATGAA GCACATTCAT
    GCACAGCTGA ACCTGGGTTC TCTGAAGCCG GGAAGGGCAG TGCTGAGGAA AAAGGAGGAT
    GGTTTTATTA AAGAATTAAG CATTGCCATG CAGCTCCTAA GAAACTGTCT TTATCAGAAT
    GAAGAATGTA AAGGGGCGGC TCTGGACGCT CACCTGGCCC CTGTCCTGCA CGCTCTCTGG
    CCTTGGCTCT TGATGGATGA CTCACTGATG CAGGTTGCGC TGCAGCTGCT TTGTGTCTAC
    ACCGCAAATT TCCCAAATGG TTGCAGTTCC CTCTGTTGGT CAAGTTATGG ACAGCATCCT
    GTTCAAGCCG CACACCGAGG GGCCCCCGGC AGCTCCCTGA TGCTGTGTAT CCTGAAGATG
    GCTTCCCAGG TGCCTCCAGA GAACACGGCT GTTCAGCAGG CAGTGTTCAT GATTCTTTCA
    AACCTGGCCT TGTCTCATGA CTGTAAAGGG GTAATTCAGA AAAGTAACTT CCTGCAGAAC
    TTTCTGTCTC TGACGTTGCC GAAAGGGGGA AACAAACATC TGAGTAACCT GAGCGTGCTC
    TGGCTGAAGC TGCTACTGAA CATGTCCTTC GAAGAAGATG GGCAGCAGGT GATCCTGCGG
    CTGGACGGCT GTCTAGACCT GCTCACAGAG ATGAGCAGGT TCAAGCACAA GAGCAGCCTC
    TCCATCCCCC TTCTTATTTT CCATAACATC TGCTTCAGTC CTGCCAACAA GCCCAAGATC
    CTGGCTAACG AGAAAGTCAT TAGTGTGCTC GCTGCCTGTC TGGAAAGTGA GAATCAAAAC
    GCTCAGAGGA TTGGAGCAGC CGCACTGTGG GCTCTCATTC ACAATTACCA GAAGGCAAAA
    ACAACTTTGA AAAACCCATC AATAAAAAGA AGAGTGGAAG AAGCATATTC CTTAGCAAAG
    AAAACTTTCT CAGACTCAGA AGAAACCCCT CTAAGTGCCT ATTATTTGAA ATGTCTTGAA
    AACCTTGTGC AGCAGCTTAA CTCTTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224096.1
    CDS281..6970
    Translation

    Target ORF information:

    RefSeq Version XM_005224039.3
    Organism Bos taurus(cattle)
    Definition Bos taurus rotatin (RTTN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224039.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGTCCTGA CGGGGCTCAT CCGGAAACTC GGTCACCCGC TGGCCGAGAT CCGGGAGCGC 
    GCGCTCAGGA GTATTCTCTG CAAGCTCGAG CACAGCTTGG TCTGCGGCGC GGACCTGGCT
    TCGCACAGGC TGCTGTTTCT CCACTTGCTG GAATGGTTCA ATTTCCCGTC CGTCCCAGTG
    AAGGACGAGG TCCTGGGTCT GCTGAGCAGA CTGGTTAAGT ATCCTCTAGC AGTGCAGCAT
    TTGGTTGATC TTGGCGCGGT CGAATTCTTA TCTAAACTTC GCCCTAATGT GGAGCCGAAT
    CTACAGGCAG AAATTGATGG CATTCTGGAT GGACTTTTTA GTCTTCCTTC AGAAGTTCCT
    GAAATGTATT CTTCATCTTA CCAGACCAGT CAAACTGATT TGCTGCAACA ACCTGAAATC
    TTAACAGGAT ATTTTCCTCA AGACAAAAGT ACTTTGCAGC AGACAGAAAT ACCTTCACGA
    CCTGTGGCAG CAAATCCGAC CGCAAAGTGT TTGAAGTTTT CTACTTTCCC CTGGCTCCCC
    CTGACCGCAG CTGACAGACA CGTTCTCTCG TCCAACGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTGTAA TCTGGAACAC CTGTGAACTG TTGAAAGACG TTATCATGCA AGATTTTCCT
    GCTGAGATAT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGAG GAGACGGGAA GCATCGTCTG GCATTACAGT CAGTGTCCTG CCTGCAGCAG
    CTGTGCACGT GTTTAAGAAA CAGACTCAAT TTCCACAGAG ATCCAAGTTT CTTCTCTAAT
    AAACAAGATG CCGTGTCCCA GAACTCTTCC CTGTCTTACT GTCCTGATGC CAGGGCGGCT
    CCGCATTCCC AGAACCCGTC TCCCGGAAGC AGCAGCCCGC GGCCCTCCGT GGTGGGGCGC
    ACGGGCCAGC GGCCCCGGGG GGACGGCCAG GACTGGGACG CCGTGTCCTC CAGTGGGAGT
    AGCAGCCACG CTCACGTCAG CTCGCGGGCC TCTGCCCCCT CGCCTCTGGA CCTGGCGCCC
    GTGGACCTGC CGGAGCTGGC AGAGGACGCG CTGGAGCTGC AGCTGCAGCA GCTGAGCCTG
    CCCCAGCTCT GCGTCTCCCT CCTGGAGGCC GCCGCGCCCC TCCTGAGGAC AGGTAGTAGA
    CAGATGATAA TAAGAGTTCT GGAGTTGCTC GCAGAAGTTG TGGTGCTTAT TGGTGAAGCG
    ATTTCAGCAC ATCTGTGGGA TGACAGCAGT CTTTTTGCAA TAGATATGAA AGAGAAGCTG
    CTCGTGGTGT TGGCGTCCCT TGGAGACACC GTGTGCTACC ATAAAAGCTG TGTCAGTCTG
    GAGCAGCCGG AGGCGCTGGT CGTGCACCAC AGGATGGCCT TTGTCAGCAT CTCGCTGTTT
    GCTGTCCGGC TCCTGCAGAC GCTGCTCCCT GTGGAAAAGG CAAGCCGCTT TTTACCGGAG
    CCTATGTTAG CAGCATTATT TTTTATTTCT TTGGACATGC CTGTTTCTTT GGAATATCCA
    AACATTCACG AAGCTGTTGT GGCCTATTTG GAACAGCTGA ATTCTGAAAA CTACAGTATT
    TATAAACGAA CTGCAGAGGC TGCTTATTCA ATTGAGTGTA CCTGTAACTT CCTGTCTGAT
    GTTGGGAAGG AGGGAGAAAA GAATCTCTCA GAGTTAGTGG AGCTGGCAGA TCAAGCCTTA
    CGTAGCTTTT CCTACCATCA GCATTTCCCC CTAATAAAGG AAATCATCTC CATTTGTTCA
    AAAATCTGGA AATCCGTGCA GGCCAGTCCT TTACTACAAG GAGAAAGTCA GAAGGTGTTT
    CTGCGGATGT TGTCTCACCC TTTGCTACAA GTGAAAGTTG AAACTTACCA CTGCTGTCTG
    GAAATTGTTA AGGAATGTTT AGGCATCCAT AATATCAGTA AACCTGTGTC CTCCCTTTGT
    AATGGAATTC ATTTTCTACT TCACCCAAAA GTTCTGTATG AAATCTCTGC ATTTGGCATT
    CAAGACCCCA AAAATGAGGT GAGCGCCGCA GCCAAGGCCA TCCTGGTGCA CCTGCTGCAG
    GGCCGACTGA GGGTGTCGGC GCCGACCTGG AACAAGTTCA TCGAGTCCCT GTGCCCCGTC
    ATCCCCGTGC TGCAGGGCTA CGCCGACACT GAAGACCCTC TGGGGAACTG CATCCTCCAC
    CTCAGCAGAG AGAGTCCAGA GACCGATGAA GGGGTGCTCC CCTCTGCCAC CCGGCTGAAG
    TCCATGCTGC GGCTCCTGCT GGTGAAGAAG CCCTCGGTCC GGGTGCTGGC ATTAAAGCTC
    TTAGCATCTC ACCTCACAAG GGAAGAGGGG TCTGACACCA AGCGCCCTTC AATAGATGCC
    AGAGTTCTCT CCAGAGTTAC TAATTTATTT ATTGTGAAAA AACCTATTGA ACTCAAATTG
    GATGACCGAA GAGAACTGAT GATTAAGTTG GAGACTGTTG AAAAGGTGTA TGGAATCTTT
    GTCTCAGATG ATGTTGATCT TGTTTTGAGA AAGTCAGCCG CAGAGCAGTT AGTGGTGATT
    ATGCAAGATA TTAAAATGCA TGCTGTGGTG AAAAAAGTGC ACCTAGTTGA CAAGATAATT
    GAGTATTTAA GTGAATGTGT GGGTCAGGAT GGTGAGGTGG TGGAGTGTTT GGTTCAGCCG
    AGCCTCATGC TCCTGAGGAA GGTCCTCTGT GCCGACCCCG TCCTGCGAGA GTCTCTGTCC
    CAGAAGACTT CTGTCCTGAC CCTGCTCTTC AGAGTTTCCT TGATATTTCA TGAAGATTGT
    ACCATTGTGA CTGAAGTTGG AGCATTGTTT TGTCTTCTGT TATTTGATGA AGTATCAAGA
    ATGGATATGT GGTCTGATAG TCCTTCCTGC ACACCATCTT CACCATCAGT CTTCAGTCTG
    CCTGTTTCAG TTTCTAGAAG GTATCATCTA CCAGTGCATG TAGTTGGCCA CCATGCCGTG
    AGTCCTTACT CCATGGTTTT GCCGTTATCT GCTGACTCTT TGGCCTTGAA GCCGGTATCA
    GATATGCTGA GGATGGCTTG GAACCTGTCG TGGTATCACG GGAGTGACAA TGTGTTGAAG
    CAAGTGAACT CCGAAGCTGA AATCCAGGAA TTTTTAGATA CACTGAAGTT ATCCACCGAG
    GACACCCTTA CTCTGAAGAT GACCCACACA CCTAGTGGAC TCAAGGACTG TCTCTGCTCC
    ATTGTTCAGG CCGCAGGCCA CAGGGAAGTG AGGGCTGCCC TCACAAGGAT GAGTTTTTAT
    CTCCTAAATG ACAGACTGTC TTTCAAGGGC GGCACTGGCC CCTGTGGGGT TACCCTGAAG
    TCCTTGTCTT GGCACACCAC ACTAAACAGG TTTTTACAAG TTCTTCCTGC TTGCACTGAA
    GATGAGAAGC TGTTAATAGA TATCATACAT TTTCTCAGTA AGTTATTGAA GGAACAAAGG
    AAAAATGTAC CCGTAGAACT TCTAAACTGG CTTCTAGAAG TACTTCTTAG ACAAAGTCCA
    AATCCACTGT TGGACCTGCT GGTTCTGACG GAGTCCCAGA CACGAGAGGA AACAGATGAT
    GTCCGCACTG CGGTCAGGCA GCAGCTCCAG AAAGAGCTGA TTGCTCTCTT TAGTACCTTG
    CTGCTCAGTT TTGAGGCTGT TACTGACAGG AAATGCTTGG AGCTTTTTTA CGTTTTCCAA
    ACGCAGCTGG CCCTGAAACT ACTCCAGTGT CTGAGAGTCA CGGACGCTCC TCACTTCTAT
    GGCCTACCGT CCCTTGAGAG GACCTTGCGG GGGATGGCCC ACCTCACGGC GTGTCCAGGG
    TGGAGCACAC ACTCTCCTCT GACAAAGCCA CTCGACATTT GTGGGAAATA CTTGTCAGGT
    CTCCTCGAAG TCATCACATC CTTCTACGTG GAGCGAGGAG GAAATGCGAT GTCCTTCATG
    GGAAAAGGTG TCACAAAGAG CACCGTTCTC TGCCTGCTCC ACTTGTCGCA TGAGATGATG
    GCCCAGGCCC AGAGCTCGGG GTGGATGTCA CTTTGGTTCT TGCCTTTGGG GAGTCAGAGT
    GAAGACCATG CTCCCACTCA GCAGGGATTG GCTTGGCTGA TTCCGTTGTG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTAACCAC CCTTGAAACT
    GGCTGTGTGG CCTTGGCAAA CAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGAACGGTG
    GTGAACATTC TTCTGGACCA GTCGGAATGC AGCATGGTGC GCCGGGAGGC TGCATTTATT
    TTTCAGAATC TCCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGCCCCTGTG TCCATGATGA GGACTCTGGG CTGTCTCTCA TAGGAAAACC TGCCCTCCAG
    GCTCTTTTAT ACCACTGCCA TTTTTATGAA CATTTGAATC AGATGGTAAA ACATTGTTAC
    CTGGGACGGT ATGCATTTGA TTTCAGTTTT TCAGCTCTTA ATGGAGCTCC TGAAGGCAAT
    GATTTAAATG GTTTAGATGA CTCGTTCAAG TTCTGGAGGG CTCCATCTCG AATGTCCAGT
    CAAGATCGAG ATCCAAGTTC TCTCTCTACC TCGGAAACAA TGGTGGCAGC TTCTTCGGGC
    AGTGCTGAAT TTCAGTCCCT CGTGCTCTCC ATGGTGCCTC ACCCTGAAGT CACACACGAC
    CAGCTGGTGG CCCAAGGTCA GCGTGAAGCT GCATCTCCAC GGCTTCCTCC TGATTCACCC
    CTCTCTGCTC CTCTGCCCAG GCAGTGTGTG TTTGTCACCC CGCCCCTCCT CTCCGCGATG
    TGCAGCCTCC TAGACAGCCT CTTGGTGGCC ACTCCAGGCG ACACTGCCGG CGCTTTCCGA
    CGAACCCGTC TCATTGAGCT TCTCTGTAGC ATCGCAGTTG CCACCCTCCT AGAGACAAGT
    GTCCAGGAGC TCCGGGCCTC GCTGCCTACA TCTCTTCCTG CTGAACACAC TCAGGCTCAG
    GTGTCCTTTC TCCTGGAATA CTTGTCCTCC TTATCCCGGC TCCTGCAGTC ATGTTTATTG
    GTGGATCCCG ACCTTGTGAT TCAGGATGAG CTTCTGAAAC CTCTTATCCC AAACATCATC
    CGAGTTCTCA CCCTATGCAC CAAAGATATA TTAGATGTAG AGTTAAGGTC AGCTTTTTAT
    CAGACATGGA CTCATCTGTT CAACCTTCTG GCCGCACTGC TGAGGCGAGC TGGCCCTGCC
    TCTTTCCCAT ATGTTACCCT GGGTCTGGCC CGGTCTTGGG CAGCGGTGAT CGATACATTC
    TGCAGGTGTG CGCGTTTGTC TACCACAAGC CCTGCTCTGT ACACCGCCAG CCTGCAGTTC
    CTTTCTGTTC TCTTGACCGA AGAAGCAAAA AGGCGTCTCC AGGATGAGGA GAAAACAAGC
    TTATGCCACA GTCCAACAGT GGCTTCACTT CTTGACAAAA CCCAGGAAAG TCAGAACTCA
    CTAGAACGAC TCACTGAAGT CATTCTTCAG TGCTATGAGG GGAAAACCTC CAAAGATGTC
    CTGAAGAGGG TCGCTGCGAA CGCACTGATG TCGCTTCTGG CCGTCAGCAG AAGAGCACAG
    AGACATGCGC TGAAGGCCCA TCTTGTAGAC AGCTGCGTGG AGCAGATGAA GCACATTCAT
    GCACAGCTGA ACCTGGGTTC TCTGAAGCCG GGAAGGGCAG TGCTGAGGAA AAAGGAGGAT
    GGTTTTATTA AAGAATTAAG CATTGCCATG CAGCTCCTAA GAAACTGTCT TTATCAGAAT
    GAAGAATGTA AAGGGGCGGC TCTGGACGCT CACCTGGCCC CTGTCCTGCA CGCTCTCTGG
    CCTTGGCTCT TGATGGATGA CTCACTGATG CAGGTTGCGC TGCAGCTGCT TTGTGTCTAC
    ACCGCAAATT TCCCAAATGG TTGCAGTTCC CTCTGTTGGT CAAGTTATGG ACAGCATCCT
    GTTCAAGCCG CACACCGAGG GGCCCCCGGC AGCTCCCTGA TGCTGTGTAT CCTGAAGATG
    GCTTCCCAGG TGCCTCCAGA GAACACGGCT GTTCAGCAGG CAGTGTTCAT GATTCTTTCA
    AACCTGGCCT TGTCTCATGA CTGTAAAGGG GTAATTCAGA AAAGTAACTT CCTGCAGAAC
    TTTCTGTCTC TGACGTTGCC GAAAGGGGGA AACAAACATC TGAGTAACCT GAGCGTGCTC
    TGGCTGAAGC TGCTACTGAA CATGTCCTTC GAAGAAGATG GGCAGCAGGT GATCCTGCGG
    CTGGACGGCT GTCTAGACCT GCTCACAGAG ATGAGCAGGT TCAAGCACAA GAGCAGCCTC
    TCCATCCCCC TTCTTATTTT CCATAACATC TGCTTCAGTC CTGCCAACAA GCCCAAGATC
    CTGGCTAACG AGAAAGTCAT TAGTGTGCTC GCTGCCTGTC TGGAAAGTGA GAATCAAAAC
    GCTCAGAGGA TTGGAGCAGC CGCACTGTGG GCTCTCATTC ACAATTACCA GAAGGCAAAA
    ACAACTTTGA AAAACCCATC AATAAAAAGA AGAGTGGAAG AAGCATATTC CTTAGCAAAG
    AAAACTTTCT CAGACTCAGA AGAAACCCCT CTAAGTGCCT ATTATTTGAA ATGTCTTGAA
    AACCTTGTGC AGCAGCTTAA CTCTTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32440
    Clone ID Related Accession (Same CDS sequence) XM_005224039.3 , XM_005224039.4
    Accession Version XM_005224039.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rotatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224039.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGTCCTGA CGGGGCTCAT CCGGAAACTC GGTCACCCGC TGGCCGAGAT CCGGGAGCGC 
    GCGCTCAGGA GTATTCTCTG CAAGCTCGAG CACAGCTTGG TCTGCGGCGC GGACCTGGCT
    TCGCACAGGC TGCTGTTTCT CCACTTGCTG GAATGGTTCA ATTTCCCGTC CGTCCCAGTG
    AAGGACGAGG TCCTGGGTCT GCTGAGCAGA CTGGTTAAGT ATCCTCTAGC AGTGCAGCAT
    TTGGTTGATC TTGGCGCGGT CGAATTCTTA TCTAAACTTC GCCCTAATGT GGAGCCGAAT
    CTACAGGCAG AAATTGATGG CATTCTGGAT GGACTTTTTA GTCTTCCTTC AGAAGTTCCT
    GAAATGTATT CTTCATCTTA CCAGACCAGT CAAACTGATT TGCTGCAACA ACCTGAAATC
    TTAACAGGAT ATTTTCCTCA AGACAAAAGT ACTTTGCAGC AGACAGAAAT ACCTTCACGA
    CCTGTGGCAG CAAATCCGAC CGCAAAGTGT TTGAAGTTTT CTACTTTCCC CTGGCTCCCC
    CTGACCGCAG CTGACAGACA CGTTCTCTCG TCCAACGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTGTAA TCTGGAACAC CTGTGAACTG TTGAAAGACG TTATCATGCA AGATTTTCCT
    GCTGAGATAT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGAG GAGACGGGAA GCATCGTCTG GCATTACAGT CAGTGTCCTG CCTGCAGCAG
    CTGTGCACGT GTTTAAGAAA CAGACTCAAT TTCCACAGAG ATCCAAGTTT CTTCTCTAAT
    AAACAAGATG CCGTGTCCCA GAACTCTTCC CTGTCTTACT GTCCTGATGC CAGGGCGGCT
    CCGCATTCCC AGAACCCGTC TCCCGGAAGC AGCAGCCCGC GGCCCTCCGT GGTGGGGCGC
    ACGGGCCAGC GGCCCCGGGG GGACGGCCAG GACTGGGACG CCGTGTCCTC CAGTGGGAGT
    AGCAGCCACG CTCACGTCAG CTCGCGGGCC TCTGCCCCCT CGCCTCTGGA CCTGGCGCCC
    GTGGACCTGC CGGAGCTGGC AGAGGACGCG CTGGAGCTGC AGCTGCAGCA GCTGAGCCTG
    CCCCAGCTCT GCGTCTCCCT CCTGGAGGCC GCCGCGCCCC TCCTGAGGAC AGGTAGTAGA
    CAGATGATAA TAAGAGTTCT GGAGTTGCTC GCAGAAGTTG TGGTGCTTAT TGGTGAAGCG
    ATTTCAGCAC ATCTGTGGGA TGACAGCAGT CTTTTTGCAA TAGATATGAA AGAGAAGCTG
    CTCGTGGTGT TGGCGTCCCT TGGAGACACC GTGTGCTACC ATAAAAGCTG TGTCAGTCTG
    GAGCAGCCGG AGGCGCTGGT CGTGCACCAC AGGATGGCCT TTGTCAGCAT CTCGCTGTTT
    GCTGTCCGGC TCCTGCAGAC GCTGCTCCCT GTGGAAAAGG CAAGCCGCTT TTTACCGGAG
    CCTATGTTAG CAGCATTATT TTTTATTTCT TTGGACATGC CTGTTTCTTT GGAATATCCA
    AACATTCACG AAGCTGTTGT GGCCTATTTG GAACAGCTGA ATTCTGAAAA CTACAGTATT
    TATAAACGAA CTGCAGAGGC TGCTTATTCA ATTGAGTGTA CCTGTAACTT CCTGTCTGAT
    GTTGGGAAGG AGGGAGAAAA GAATCTCTCA GAGTTAGTGG AGCTGGCAGA TCAAGCCTTA
    CGTAGCTTTT CCTACCATCA GCATTTCCCC CTAATAAAGG AAATCATCTC CATTTGTTCA
    AAAATCTGGA AATCCGTGCA GGCCAGTCCT TTACTACAAG GAGAAAGTCA GAAGGTGTTT
    CTGCGGATGT TGTCTCACCC TTTGCTACAA GTGAAAGTTG AAACTTACCA CTGCTGTCTG
    GAAATTGTTA AGGAATGTTT AGGCATCCAT AATATCAGTA AACCTGTGTC CTCCCTTTGT
    AATGGAATTC ATTTTCTACT TCACCCAAAA GTTCTGTATG AAATCTCTGC ATTTGGCATT
    CAAGACCCCA AAAATGAGGT GAGCGCCGCA GCCAAGGCCA TCCTGGTGCA CCTGCTGCAG
    GGCCGACTGA GGGTGTCGGC GCCGACCTGG AACAAGTTCA TCGAGTCCCT GTGCCCCGTC
    ATCCCCGTGC TGCAGGGCTA CGCCGACACT GAAGACCCTC TGGGGAACTG CATCCTCCAC
    CTCAGCAGAG AGAGTCCAGA GACCGATGAA GGGGTGCTCC CCTCTGCCAC CCGGCTGAAG
    TCCATGCTGC GGCTCCTGCT GGTGAAGAAG CCCTCGGTCC GGGTGCTGGC ATTAAAGCTC
    TTAGCATCTC ACCTCACAAG GGAAGAGGGG TCTGACACCA AGCGCCCTTC AATAGATGCC
    AGAGTTCTCT CCAGAGTTAC TAATTTATTT ATTGTGAAAA AACCTATTGA ACTCAAATTG
    GATGACCGAA GAGAACTGAT GATTAAGTTG GAGACTGTTG AAAAGGTGTA TGGAATCTTT
    GTCTCAGATG ATGTTGATCT TGTTTTGAGA AAGTCAGCCG CAGAGCAGTT AGTGGTGATT
    ATGCAAGATA TTAAAATGCA TGCTGTGGTG AAAAAAGTGC ACCTAGTTGA CAAGATAATT
    GAGTATTTAA GTGAATGTGT GGGTCAGGAT GGTGAGGTGG TGGAGTGTTT GGTTCAGCCG
    AGCCTCATGC TCCTGAGGAA GGTCCTCTGT GCCGACCCCG TCCTGCGAGA GTCTCTGTCC
    CAGAAGACTT CTGTCCTGAC CCTGCTCTTC AGAGTTTCCT TGATATTTCA TGAAGATTGT
    ACCATTGTGA CTGAAGTTGG AGCATTGTTT TGTCTTCTGT TATTTGATGA AGTATCAAGA
    ATGGATATGT GGTCTGATAG TCCTTCCTGC ACACCATCTT CACCATCAGT CTTCAGTCTG
    CCTGTTTCAG TTTCTAGAAG GTATCATCTA CCAGTGCATG TAGTTGGCCA CCATGCCGTG
    AGTCCTTACT CCATGGTTTT GCCGTTATCT GCTGACTCTT TGGCCTTGAA GCCGGTATCA
    GATATGCTGA GGATGGCTTG GAACCTGTCG TGGTATCACG GGAGTGACAA TGTGTTGAAG
    CAAGTGAACT CCGAAGCTGA AATCCAGGAA TTTTTAGATA CACTGAAGTT ATCCACCGAG
    GACACCCTTA CTCTGAAGAT GACCCACACA CCTAGTGGAC TCAAGGACTG TCTCTGCTCC
    ATTGTTCAGG CCGCAGGCCA CAGGGAAGTG AGGGCTGCCC TCACAAGGAT GAGTTTTTAT
    CTCCTAAATG ACAGACTGTC TTTCAAGGGC GGCACTGGCC CCTGTGGGGT TACCCTGAAG
    TCCTTGTCTT GGCACACCAC ACTAAACAGG TTTTTACAAG TTCTTCCTGC TTGCACTGAA
    GATGAGAAGC TGTTAATAGA TATCATACAT TTTCTCAGTA AGTTATTGAA GGAACAAAGG
    AAAAATGTAC CCGTAGAACT TCTAAACTGG CTTCTAGAAG TACTTCTTAG ACAAAGTCCA
    AATCCACTGT TGGACCTGCT GGTTCTGACG GAGTCCCAGA CACGAGAGGA AACAGATGAT
    GTCCGCACTG CGGTCAGGCA GCAGCTCCAG AAAGAGCTGA TTGCTCTCTT TAGTACCTTG
    CTGCTCAGTT TTGAGGCTGT TACTGACAGG AAATGCTTGG AGCTTTTTTA CGTTTTCCAA
    ACGCAGCTGG CCCTGAAACT ACTCCAGTGT CTGAGAGTCA CGGACGCTCC TCACTTCTAT
    GGCCTACCGT CCCTTGAGAG GACCTTGCGG GGGATGGCCC ACCTCACGGC GTGTCCAGGG
    TGGAGCACAC ACTCTCCTCT GACAAAGCCA CTCGACATTT GTGGGAAATA CTTGTCAGGT
    CTCCTCGAAG TCATCACATC CTTCTACGTG GAGCGAGGAG GAAATGCGAT GTCCTTCATG
    GGAAAAGGTG TCACAAAGAG CACCGTTCTC TGCCTGCTCC ACTTGTCGCA TGAGATGATG
    GCCCAGGCCC AGAGCTCGGG GTGGATGTCA CTTTGGTTCT TGCCTTTGGG GAGTCAGAGT
    GAAGACCATG CTCCCACTCA GCAGGGATTG GCTTGGCTGA TTCCGTTGTG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTAACCAC CCTTGAAACT
    GGCTGTGTGG CCTTGGCAAA CAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGAACGGTG
    GTGAACATTC TTCTGGACCA GTCGGAATGC AGCATGGTGC GCCGGGAGGC TGCATTTATT
    TTTCAGAATC TCCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGCCCCTGTG TCCATGATGA GGACTCTGGG CTGTCTCTCA TAGGAAAACC TGCCCTCCAG
    GCTCTTTTAT ACCACTGCCA TTTTTATGAA CATTTGAATC AGATGGTAAA ACATTGTTAC
    CTGGGACGGT ATGCATTTGA TTTCAGTTTT TCAGCTCTTA ATGGAGCTCC TGAAGGCAAT
    GATTTAAATG GTTTAGATGA CTCGTTCAAG TTCTGGAGGG CTCCATCTCG AATGTCCAGT
    CAAGATCGAG ATCCAAGTTC TCTCTCTACC TCGGAAACAA TGGTGGCAGC TTCTTCGGGC
    AGTGCTGAAT TTCAGTCCCT CGTGCTCTCC ATGGTGCCTC ACCCTGAAGT CACACACGAC
    CAGCTGGTGG CCCAAGGTCA GCGTGAAGCT GCATCTCCAC GGCTTCCTCC TGATTCACCC
    CTCTCTGCTC CTCTGCCCAG GCAGTGTGTG TTTGTCACCC CGCCCCTCCT CTCCGCGATG
    TGCAGCCTCC TAGACAGCCT CTTGGTGGCC ACTCCAGGCG ACACTGCCGG CGCTTTCCGA
    CGAACCCGTC TCATTGAGCT TCTCTGTAGC ATCGCAGTTG CCACCCTCCT AGAGACAAGT
    GTCCAGGAGC TCCGGGCCTC GCTGCCTACA TCTCTTCCTG CTGAACACAC TCAGGCTCAG
    GTGTCCTTTC TCCTGGAATA CTTGTCCTCC TTATCCCGGC TCCTGCAGTC ATGTTTATTG
    GTGGATCCCG ACCTTGTGAT TCAGGATGAG CTTCTGAAAC CTCTTATCCC AAACATCATC
    CGAGTTCTCA CCCTATGCAC CAAAGATATA TTAGATGTAG AGTTAAGGTC AGCTTTTTAT
    CAGACATGGA CTCATCTGTT CAACCTTCTG GCCGCACTGC TGAGGCGAGC TGGCCCTGCC
    TCTTTCCCAT ATGTTACCCT GGGTCTGGCC CGGTCTTGGG CAGCGGTGAT CGATACATTC
    TGCAGGTGTG CGCGTTTGTC TACCACAAGC CCTGCTCTGT ACACCGCCAG CCTGCAGTTC
    CTTTCTGTTC TCTTGACCGA AGAAGCAAAA AGGCGTCTCC AGGATGAGGA GAAAACAAGC
    TTATGCCACA GTCCAACAGT GGCTTCACTT CTTGACAAAA CCCAGGAAAG TCAGAACTCA
    CTAGAACGAC TCACTGAAGT CATTCTTCAG TGCTATGAGG GGAAAACCTC CAAAGATGTC
    CTGAAGAGGG TCGCTGCGAA CGCACTGATG TCGCTTCTGG CCGTCAGCAG AAGAGCACAG
    AGACATGCGC TGAAGGCCCA TCTTGTAGAC AGCTGCGTGG AGCAGATGAA GCACATTCAT
    GCACAGCTGA ACCTGGGTTC TCTGAAGCCG GGAAGGGCAG TGCTGAGGAA AAAGGAGGAT
    GGTTTTATTA AAGAATTAAG CATTGCCATG CAGCTCCTAA GAAACTGTCT TTATCAGAAT
    GAAGAATGTA AAGGGGCGGC TCTGGACGCT CACCTGGCCC CTGTCCTGCA CGCTCTCTGG
    CCTTGGCTCT TGATGGATGA CTCACTGATG CAGGTTGCGC TGCAGCTGCT TTGTGTCTAC
    ACCGCAAATT TCCCAAATGG TTGCAGTTCC CTCTGTTGGT CAAGTTATGG ACAGCATCCT
    GTTCAAGCCG CACACCGAGG GGCCCCCGGC AGCTCCCTGA TGCTGTGTAT CCTGAAGATG
    GCTTCCCAGG TGCCTCCAGA GAACACGGCT GTTCAGCAGG CAGTGTTCAT GATTCTTTCA
    AACCTGGCCT TGTCTCATGA CTGTAAAGGG GTAATTCAGA AAAGTAACTT CCTGCAGAAC
    TTTCTGTCTC TGACGTTGCC GAAAGGGGGA AACAAACATC TGAGTAACCT GAGCGTGCTC
    TGGCTGAAGC TGCTACTGAA CATGTCCTTC GAAGAAGATG GGCAGCAGGT GATCCTGCGG
    CTGGACGGCT GTCTAGACCT GCTCACAGAG ATGAGCAGGT TCAAGCACAA GAGCAGCCTC
    TCCATCCCCC TTCTTATTTT CCATAACATC TGCTTCAGTC CTGCCAACAA GCCCAAGATC
    CTGGCTAACG AGAAAGTCAT TAGTGTGCTC GCTGCCTGTC TGGAAAGTGA GAATCAAAAC
    GCTCAGAGGA TTGGAGCAGC CGCACTGTGG GCTCTCATTC ACAATTACCA GAAGGCAAAA
    ACAACTTTGA AAAACCCATC AATAAAAAGA AGAGTGGAAG AAGCATATTC CTTAGCAAAG
    AAAACTTTCT CAGACTCAGA AGAAACCCCT CTAAGTGCCT ATTATTTGAA ATGTCTTGAA
    AACCTTGTGC AGCAGCTTAA CTCTTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224096.1
    CDS304..6993
    Translation

    Target ORF information:

    RefSeq Version XM_005224039.4
    Organism Bos taurus(cattle)
    Definition Bos taurus rotatin (RTTN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224039.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGTCCTGA CGGGGCTCAT CCGGAAACTC GGTCACCCGC TGGCCGAGAT CCGGGAGCGC 
    GCGCTCAGGA GTATTCTCTG CAAGCTCGAG CACAGCTTGG TCTGCGGCGC GGACCTGGCT
    TCGCACAGGC TGCTGTTTCT CCACTTGCTG GAATGGTTCA ATTTCCCGTC CGTCCCAGTG
    AAGGACGAGG TCCTGGGTCT GCTGAGCAGA CTGGTTAAGT ATCCTCTAGC AGTGCAGCAT
    TTGGTTGATC TTGGCGCGGT CGAATTCTTA TCTAAACTTC GCCCTAATGT GGAGCCGAAT
    CTACAGGCAG AAATTGATGG CATTCTGGAT GGACTTTTTA GTCTTCCTTC AGAAGTTCCT
    GAAATGTATT CTTCATCTTA CCAGACCAGT CAAACTGATT TGCTGCAACA ACCTGAAATC
    TTAACAGGAT ATTTTCCTCA AGACAAAAGT ACTTTGCAGC AGACAGAAAT ACCTTCACGA
    CCTGTGGCAG CAAATCCGAC CGCAAAGTGT TTGAAGTTTT CTACTTTCCC CTGGCTCCCC
    CTGACCGCAG CTGACAGACA CGTTCTCTCG TCCAACGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTGTAA TCTGGAACAC CTGTGAACTG TTGAAAGACG TTATCATGCA AGATTTTCCT
    GCTGAGATAT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGAG GAGACGGGAA GCATCGTCTG GCATTACAGT CAGTGTCCTG CCTGCAGCAG
    CTGTGCACGT GTTTAAGAAA CAGACTCAAT TTCCACAGAG ATCCAAGTTT CTTCTCTAAT
    AAACAAGATG CCGTGTCCCA GAACTCTTCC CTGTCTTACT GTCCTGATGC CAGGGCGGCT
    CCGCATTCCC AGAACCCGTC TCCCGGAAGC AGCAGCCCGC GGCCCTCCGT GGTGGGGCGC
    ACGGGCCAGC GGCCCCGGGG GGACGGCCAG GACTGGGACG CCGTGTCCTC CAGTGGGAGT
    AGCAGCCACG CTCACGTCAG CTCGCGGGCC TCTGCCCCCT CGCCTCTGGA CCTGGCGCCC
    GTGGACCTGC CGGAGCTGGC AGAGGACGCG CTGGAGCTGC AGCTGCAGCA GCTGAGCCTG
    CCCCAGCTCT GCGTCTCCCT CCTGGAGGCC GCCGCGCCCC TCCTGAGGAC AGGTAGTAGA
    CAGATGATAA TAAGAGTTCT GGAGTTGCTC GCAGAAGTTG TGGTGCTTAT TGGTGAAGCG
    ATTTCAGCAC ATCTGTGGGA TGACAGCAGT CTTTTTGCAA TAGATATGAA AGAGAAGCTG
    CTCGTGGTGT TGGCGTCCCT TGGAGACACC GTGTGCTACC ATAAAAGCTG TGTCAGTCTG
    GAGCAGCCGG AGGCGCTGGT CGTGCACCAC AGGATGGCCT TTGTCAGCAT CTCGCTGTTT
    GCTGTCCGGC TCCTGCAGAC GCTGCTCCCT GTGGAAAAGG CAAGCCGCTT TTTACCGGAG
    CCTATGTTAG CAGCATTATT TTTTATTTCT TTGGACATGC CTGTTTCTTT GGAATATCCA
    AACATTCACG AAGCTGTTGT GGCCTATTTG GAACAGCTGA ATTCTGAAAA CTACAGTATT
    TATAAACGAA CTGCAGAGGC TGCTTATTCA ATTGAGTGTA CCTGTAACTT CCTGTCTGAT
    GTTGGGAAGG AGGGAGAAAA GAATCTCTCA GAGTTAGTGG AGCTGGCAGA TCAAGCCTTA
    CGTAGCTTTT CCTACCATCA GCATTTCCCC CTAATAAAGG AAATCATCTC CATTTGTTCA
    AAAATCTGGA AATCCGTGCA GGCCAGTCCT TTACTACAAG GAGAAAGTCA GAAGGTGTTT
    CTGCGGATGT TGTCTCACCC TTTGCTACAA GTGAAAGTTG AAACTTACCA CTGCTGTCTG
    GAAATTGTTA AGGAATGTTT AGGCATCCAT AATATCAGTA AACCTGTGTC CTCCCTTTGT
    AATGGAATTC ATTTTCTACT TCACCCAAAA GTTCTGTATG AAATCTCTGC ATTTGGCATT
    CAAGACCCCA AAAATGAGGT GAGCGCCGCA GCCAAGGCCA TCCTGGTGCA CCTGCTGCAG
    GGCCGACTGA GGGTGTCGGC GCCGACCTGG AACAAGTTCA TCGAGTCCCT GTGCCCCGTC
    ATCCCCGTGC TGCAGGGCTA CGCCGACACT GAAGACCCTC TGGGGAACTG CATCCTCCAC
    CTCAGCAGAG AGAGTCCAGA GACCGATGAA GGGGTGCTCC CCTCTGCCAC CCGGCTGAAG
    TCCATGCTGC GGCTCCTGCT GGTGAAGAAG CCCTCGGTCC GGGTGCTGGC ATTAAAGCTC
    TTAGCATCTC ACCTCACAAG GGAAGAGGGG TCTGACACCA AGCGCCCTTC AATAGATGCC
    AGAGTTCTCT CCAGAGTTAC TAATTTATTT ATTGTGAAAA AACCTATTGA ACTCAAATTG
    GATGACCGAA GAGAACTGAT GATTAAGTTG GAGACTGTTG AAAAGGTGTA TGGAATCTTT
    GTCTCAGATG ATGTTGATCT TGTTTTGAGA AAGTCAGCCG CAGAGCAGTT AGTGGTGATT
    ATGCAAGATA TTAAAATGCA TGCTGTGGTG AAAAAAGTGC ACCTAGTTGA CAAGATAATT
    GAGTATTTAA GTGAATGTGT GGGTCAGGAT GGTGAGGTGG TGGAGTGTTT GGTTCAGCCG
    AGCCTCATGC TCCTGAGGAA GGTCCTCTGT GCCGACCCCG TCCTGCGAGA GTCTCTGTCC
    CAGAAGACTT CTGTCCTGAC CCTGCTCTTC AGAGTTTCCT TGATATTTCA TGAAGATTGT
    ACCATTGTGA CTGAAGTTGG AGCATTGTTT TGTCTTCTGT TATTTGATGA AGTATCAAGA
    ATGGATATGT GGTCTGATAG TCCTTCCTGC ACACCATCTT CACCATCAGT CTTCAGTCTG
    CCTGTTTCAG TTTCTAGAAG GTATCATCTA CCAGTGCATG TAGTTGGCCA CCATGCCGTG
    AGTCCTTACT CCATGGTTTT GCCGTTATCT GCTGACTCTT TGGCCTTGAA GCCGGTATCA
    GATATGCTGA GGATGGCTTG GAACCTGTCG TGGTATCACG GGAGTGACAA TGTGTTGAAG
    CAAGTGAACT CCGAAGCTGA AATCCAGGAA TTTTTAGATA CACTGAAGTT ATCCACCGAG
    GACACCCTTA CTCTGAAGAT GACCCACACA CCTAGTGGAC TCAAGGACTG TCTCTGCTCC
    ATTGTTCAGG CCGCAGGCCA CAGGGAAGTG AGGGCTGCCC TCACAAGGAT GAGTTTTTAT
    CTCCTAAATG ACAGACTGTC TTTCAAGGGC GGCACTGGCC CCTGTGGGGT TACCCTGAAG
    TCCTTGTCTT GGCACACCAC ACTAAACAGG TTTTTACAAG TTCTTCCTGC TTGCACTGAA
    GATGAGAAGC TGTTAATAGA TATCATACAT TTTCTCAGTA AGTTATTGAA GGAACAAAGG
    AAAAATGTAC CCGTAGAACT TCTAAACTGG CTTCTAGAAG TACTTCTTAG ACAAAGTCCA
    AATCCACTGT TGGACCTGCT GGTTCTGACG GAGTCCCAGA CACGAGAGGA AACAGATGAT
    GTCCGCACTG CGGTCAGGCA GCAGCTCCAG AAAGAGCTGA TTGCTCTCTT TAGTACCTTG
    CTGCTCAGTT TTGAGGCTGT TACTGACAGG AAATGCTTGG AGCTTTTTTA CGTTTTCCAA
    ACGCAGCTGG CCCTGAAACT ACTCCAGTGT CTGAGAGTCA CGGACGCTCC TCACTTCTAT
    GGCCTACCGT CCCTTGAGAG GACCTTGCGG GGGATGGCCC ACCTCACGGC GTGTCCAGGG
    TGGAGCACAC ACTCTCCTCT GACAAAGCCA CTCGACATTT GTGGGAAATA CTTGTCAGGT
    CTCCTCGAAG TCATCACATC CTTCTACGTG GAGCGAGGAG GAAATGCGAT GTCCTTCATG
    GGAAAAGGTG TCACAAAGAG CACCGTTCTC TGCCTGCTCC ACTTGTCGCA TGAGATGATG
    GCCCAGGCCC AGAGCTCGGG GTGGATGTCA CTTTGGTTCT TGCCTTTGGG GAGTCAGAGT
    GAAGACCATG CTCCCACTCA GCAGGGATTG GCTTGGCTGA TTCCGTTGTG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTAACCAC CCTTGAAACT
    GGCTGTGTGG CCTTGGCAAA CAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGAACGGTG
    GTGAACATTC TTCTGGACCA GTCGGAATGC AGCATGGTGC GCCGGGAGGC TGCATTTATT
    TTTCAGAATC TCCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGCCCCTGTG TCCATGATGA GGACTCTGGG CTGTCTCTCA TAGGAAAACC TGCCCTCCAG
    GCTCTTTTAT ACCACTGCCA TTTTTATGAA CATTTGAATC AGATGGTAAA ACATTGTTAC
    CTGGGACGGT ATGCATTTGA TTTCAGTTTT TCAGCTCTTA ATGGAGCTCC TGAAGGCAAT
    GATTTAAATG GTTTAGATGA CTCGTTCAAG TTCTGGAGGG CTCCATCTCG AATGTCCAGT
    CAAGATCGAG ATCCAAGTTC TCTCTCTACC TCGGAAACAA TGGTGGCAGC TTCTTCGGGC
    AGTGCTGAAT TTCAGTCCCT CGTGCTCTCC ATGGTGCCTC ACCCTGAAGT CACACACGAC
    CAGCTGGTGG CCCAAGGTCA GCGTGAAGCT GCATCTCCAC GGCTTCCTCC TGATTCACCC
    CTCTCTGCTC CTCTGCCCAG GCAGTGTGTG TTTGTCACCC CGCCCCTCCT CTCCGCGATG
    TGCAGCCTCC TAGACAGCCT CTTGGTGGCC ACTCCAGGCG ACACTGCCGG CGCTTTCCGA
    CGAACCCGTC TCATTGAGCT TCTCTGTAGC ATCGCAGTTG CCACCCTCCT AGAGACAAGT
    GTCCAGGAGC TCCGGGCCTC GCTGCCTACA TCTCTTCCTG CTGAACACAC TCAGGCTCAG
    GTGTCCTTTC TCCTGGAATA CTTGTCCTCC TTATCCCGGC TCCTGCAGTC ATGTTTATTG
    GTGGATCCCG ACCTTGTGAT TCAGGATGAG CTTCTGAAAC CTCTTATCCC AAACATCATC
    CGAGTTCTCA CCCTATGCAC CAAAGATATA TTAGATGTAG AGTTAAGGTC AGCTTTTTAT
    CAGACATGGA CTCATCTGTT CAACCTTCTG GCCGCACTGC TGAGGCGAGC TGGCCCTGCC
    TCTTTCCCAT ATGTTACCCT GGGTCTGGCC CGGTCTTGGG CAGCGGTGAT CGATACATTC
    TGCAGGTGTG CGCGTTTGTC TACCACAAGC CCTGCTCTGT ACACCGCCAG CCTGCAGTTC
    CTTTCTGTTC TCTTGACCGA AGAAGCAAAA AGGCGTCTCC AGGATGAGGA GAAAACAAGC
    TTATGCCACA GTCCAACAGT GGCTTCACTT CTTGACAAAA CCCAGGAAAG TCAGAACTCA
    CTAGAACGAC TCACTGAAGT CATTCTTCAG TGCTATGAGG GGAAAACCTC CAAAGATGTC
    CTGAAGAGGG TCGCTGCGAA CGCACTGATG TCGCTTCTGG CCGTCAGCAG AAGAGCACAG
    AGACATGCGC TGAAGGCCCA TCTTGTAGAC AGCTGCGTGG AGCAGATGAA GCACATTCAT
    GCACAGCTGA ACCTGGGTTC TCTGAAGCCG GGAAGGGCAG TGCTGAGGAA AAAGGAGGAT
    GGTTTTATTA AAGAATTAAG CATTGCCATG CAGCTCCTAA GAAACTGTCT TTATCAGAAT
    GAAGAATGTA AAGGGGCGGC TCTGGACGCT CACCTGGCCC CTGTCCTGCA CGCTCTCTGG
    CCTTGGCTCT TGATGGATGA CTCACTGATG CAGGTTGCGC TGCAGCTGCT TTGTGTCTAC
    ACCGCAAATT TCCCAAATGG TTGCAGTTCC CTCTGTTGGT CAAGTTATGG ACAGCATCCT
    GTTCAAGCCG CACACCGAGG GGCCCCCGGC AGCTCCCTGA TGCTGTGTAT CCTGAAGATG
    GCTTCCCAGG TGCCTCCAGA GAACACGGCT GTTCAGCAGG CAGTGTTCAT GATTCTTTCA
    AACCTGGCCT TGTCTCATGA CTGTAAAGGG GTAATTCAGA AAAGTAACTT CCTGCAGAAC
    TTTCTGTCTC TGACGTTGCC GAAAGGGGGA AACAAACATC TGAGTAACCT GAGCGTGCTC
    TGGCTGAAGC TGCTACTGAA CATGTCCTTC GAAGAAGATG GGCAGCAGGT GATCCTGCGG
    CTGGACGGCT GTCTAGACCT GCTCACAGAG ATGAGCAGGT TCAAGCACAA GAGCAGCCTC
    TCCATCCCCC TTCTTATTTT CCATAACATC TGCTTCAGTC CTGCCAACAA GCCCAAGATC
    CTGGCTAACG AGAAAGTCAT TAGTGTGCTC GCTGCCTGTC TGGAAAGTGA GAATCAAAAC
    GCTCAGAGGA TTGGAGCAGC CGCACTGTGG GCTCTCATTC ACAATTACCA GAAGGCAAAA
    ACAACTTTGA AAAACCCATC AATAAAAAGA AGAGTGGAAG AAGCATATTC CTTAGCAAAG
    AAAACTTTCT CAGACTCAGA AGAAACCCCT CTAAGTGCCT ATTATTTGAA ATGTCTTGAA
    AACCTTGTGC AGCAGCTTAA CTCTTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.