PARP4 cDNA ORF clone, Bos taurus(cattle)

The following PARP4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PARP4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa221292 XM_002691868.6
Latest version!
Bos taurus poly(ADP-ribose) polymerase family member 4 (PARP4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24788 XM_002691868.5
Latest version!
Bos taurus poly(ADP-ribose) polymerase family member 4 (PARP4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa24788 XM_867149.8
Latest version!
Bos taurus poly(ADP-ribose) polymerase family member 4 (PARP4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa92120 XM_015465709.1
Latest version!
Bos taurus poly(ADP-ribose) polymerase family member 4 (PARP4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa92120 XM_015473840.1
Latest version!
Bos taurus poly(ADP-ribose) polymerase family member 4 (PARP4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa221292
    Clone ID Related Accession (Same CDS sequence) XM_002691868.6
    Accession Version XM_002691868.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product poly [ADP-ribose] polymerase 4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002691868.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG GAATCTTCGC AAACTGTACC TTCTGTTTGA AAGCCAAGCA CTTAACTCGT 
    CAACAGAAGA GAAAACTACA AACTGATATT AAGGAAAATG GTGGAAATTT TTCCTTTTTA
    TTAAATGCTC AGTGCACACA TGTAATCTTA GACAATGCTG ATGTTCTGAG CCAGTATCAG
    CTGAAGTCTA TTCAGAAGAA CCATATTCTT ATTGCAAACC CAGATTTTGT ATGGGAATCT
    ATCAAAGAAA GAAGACTGCT GGATACAAAG ATTTATGGTC CCAGTGAGTC ACTGGACATC
    ACATCAGCTT CCAGTGAAGA ATGGAGCAGC CCTAAAATGG AAATGAATGA TTCTTCATTC
    CTGGACAGTT CCACAGAGAA GGAGAACACT GTTGAACTCA CTAAGTTTTA TGCAGAGGGT
    GTTGAAATTC CTCATTTTCC TCAAGATTTT GAAGTTGCAA AATATAACAT CTTGGAAAAA
    GTTGTGACAG AGGGAGGTAA GGAGACCGCC GTGGTGGAGC TGCAGTGCCT CCAGGGCCCT
    GGGGACCGTG GCTTTCTGAT ATGCACACAC TTCCTCCTGG CCACTGGCAC CCAGACCAGA
    AGGCAGTCCT CTGTGAAGAA AACCTCTGCA GACGCAAGTG AATACTATGA GAATTATATT
    GAGGAACTGA AGAAACAAGG CTTTCTACTG AGAGAACATT TTACACCTGA AGCAACCCAG
    TTAGCATCTG AAAAACTGCA AGCATTGCTT TTGGAGGAGG TAATAAACTC GAATGCCCTG
    AGCCCCGAGG TGAGCGGCTT GGTGGAGATG CTCTGGGCAG AAGCCCTCGG CCGCCTGGAG
    GACACACTGC TGAGACCTGT GGGCATGATG AGCCTGAATG ACGTGAGCAA GGCAGAGGGA
    ATTCTCCTCC TGGTAAAGGA AGCAATGAGA AATGGAGAAA CACAGGAGCA ACTGCAAACG
    ATGCTGACCG AGTTCTACAG ATTAATCCCT CACAGAGCTG CTGTGGCCGA GAAGGTCAGC
    CTGAGCCTGC TGGCTAAGAA GGAGGACCTG TGCCAGCTAA TAAGAGACAT GGTTAATGTC
    TGTGAAACTA ATTTGGCCAA ACCCAACCCC CCATCTCTTG CCAAATACCG AGCTTTGAGA
    TGCAAAATTG AGCATGTTGA GCCGAACACT GAAGAATTTT TGAGAGTAAG AGAAGAAGTG
    CTGCAGAATA ATCACAGTAA GGGTCCAGTG GACATCTTGC AGATATTTAG AGTTGGCAGA
    GTGAGTGAAA CCACAGAGTT CCAGGGCCAC CTTGGCAATG TCAGGCACTT ACTGCATGGG
    TCTCCTGTGC AGAGCTTCAT GGGAATCCTG TCCCGTGGGT TGCTTTTACC CAAAGTAGTT
    GAAGATCGTG GCATGAAGAG AACAGACATT GGAAATCTTG GGAGTGGGAT ATATTTCAGC
    GATTCAATCA GTACAAGCAT CAAGTACTCG CACCCTGGAC AGACAGATGG CGCCCGACTC
    CTGGTCGTCT GTGATGTCGC ACTCGGCAGG TGCATGGACC TGCACAAGCG AGACTTCTCC
    CTGACGGAGG CACCACCCGA GTACGACAGC GTCCACGGGG TGCGGGGAAC GGATATGGTC
    AGCTCGGACT TTGAGGATGA TGAATTTGTT GTATACAAAA CCAATCAGGT TAAAATGAAA
    TATCTTGTTA AATTTTCTGT ACCTGGAGAT GAAATAAAGG ACTTTCATCC TTGTGACAAT
    ACGGAACTAG AGGAAGACAG ACCTGAGTTT TCAAATTTTT CAGCAGTTGA AGATCACCAG
    CTTCCAGGCA GCACGCCTTT CCCCGACATC AAGGCCGGCC TCCAGGATGC CTCTGGGAAT
    TCAGTTCCTC TGGAGGACGT TCACATCAAG GGGAAAATAA TAGACTTTGT AGCCCAGGTC
    ATTGTTTTTC AAACATACAC AAACCAAAGT CATGTGCCCA TTGAGGCAAA ATATGTTTTT
    CCTTTGGACG ACAAGGCAGC TGTATGTGGT TTCGAGGCTT TCATCAATGG GAAGCACGTG
    GTGGGAGAGA TTAAAGAGAA GGAAGTGGCC CAGCGAGAGT ACCGAGTGGC GATCAGTCAA
    GGTCATGGCG CCTACCTGAT GGATCAGGAC ACACCCGACA TCTTCACCGT CAGCGTCGGG
    AACTTACCCC CAAAGGCCAA AGTCCTGGTC AAAATCACCT ACATCACGGA GCTGAGCATC
    CAGGGTGCCT GCGCGGTCTT CTTCCTGCCG GCCACGGTGG CGCCCTGGCA GCAGGACAGG
    GCTCTGAGGG AGAACCTTCA GGATACAGTG GAGAAGATTT GTATAAAGGA AATAGGAGCA
    AAGCAAAGCT TTTCTCTGAG CATGTCTATC GAGATGCCGC ACATGATTGA ATTCATCTCC
    AGTGACACAC ACGACCTGAG AAAAAAGAGA ACAGACTGCA AAGCTGTGAT TAGTACTGTG
    GAAGGCAGCT CCTTAGACAG CAATGGATTT TCTCTCCTCA TCGGTTTGGT TGATGTGTAT
    CTCCCAAGAA TGTGGGTTGA AAAGCATCCA GAAAAAGAAA GCGAGGCCTG CATGCTTGTG
    TTTCAACCAA ATCTTGGTAT TACCGTCCCT GACGAAGCTG AGAGGAGTGA AGTGATTATT
    TGTCTTGATT GCTCCAATTC CATGGAGGGC GAGACATTCC TGCAAGCCAA ACAGATTGCC
    TTGCATGCAC TGTCCTGTGT GGGAAAGGAG CAGAAGGTGA ACGTCATCAA GTTCGGCACA
    AGTTACAAGG AATTATTTTC ATATCCTAAG TGCATCACGA GCAATAAGAT GCCGACAGAG
    TTCATCATGT CTGCTACACC TACCATGGGA AACACAGATT TCTGGAAGAC ACTTCGATAC
    TTAAATTTGC TGTACCCTTC CCACGTGTTA CGGAACATCC TCCTCATATC CGACGGGCAC
    CTCCAGAACG AGAGCCTGAC CTTGCAGCTC GTAAGAAGAA GCGTGCCGCA CACCAGGCTG
    TTCTCCTGTG GGGTCAGTTC TACAGCAAAT CGTCATGTCT TACGGAGTCT GTCCCACTAT
    GGCGCTGGAG TATTTGAATA TTTTAACTCA AAATCCAAAC ATAGCTGGAA AAAACAGATA
    GAAGCCCAGA TGGATAGGCT GAGTTCTCCC AGTTGCCACT CGGTCTCCGT CAAATGGCAG
    CAGCTGAGCA CGGACACGCC CGCACCCCTG CAGGCCCCCA CCCAGGTGCA GTCCCTGTTC
    CACAGCGAGC GACTCCTTGT CTACGGGCTC ACTCCTCACT GTACACAGGC AACTCTACAT
    GCGCTGATTC AAGAGAGAGA GTATTCTACA GTGGTGTCAA CTACTGAACT TCAGAAGACA
    ACTGGAACGA TGATTCACAA GCTGGCAGCT CGAGCTCTGA TAAGAGATTA TGAAGATGGC
    ATTCTCCACG AAAACGAAGT GAATCACGAG ATGAAGAAGC AAACTTTAAA ATCTCTGATT
    ATTAAACTCA GTAAAGAAAA CTCTCTCATA ACACAGTTTA CAAGCTTTGT GGCTGTGGAG
    AAAAGGTATG GTAATGAGTC ACCTTCTCCT GATATTCCAA ATACTTTGGA ACTTATTGCC
    AAAGAAGACA TAGATTTTCT GCCATACATG AGCTGGGAGG GGGAACAACC AGATGCCAGC
    AGGATGCAGA CACTTTCAGC ATCGTCTGAA TGGAACCAAG AACCCAAACG TAAATGTAAA
    CGTAAACAGA CTAAAAAGAA GCGTTCATCA CTTAAGATGA TACTTTCTGA AGATTTTAAA
    GCCTTTAGCT CAGATGCCCC AGAAGAATCT GAAGCGGTCA GTTTTGTCTT CAGGGATGCA
    GAGGAGCCCT GGGGTTTGGG TCTGATGGAG TCATCTAAAC AAACAGAAGT TAAACCACAA
    TTCACAGAAG CTATCTCACA GAAAACGGCC TTTTCTGACA TTCCTACTGC CCCTAGTTTC
    TTAACTACAC CCCTCCATGT TGGCTCTGGT TTCTACCTTC AGGCCAGTTC AGCACTGTTT
    GGTTCTCCAG CTGGTCCCAA ACACTTCGGT TCCCCTAAGA AGTACCTGGA AATGGGCAGT
    GGTGCCGACG TTTCCCTGGA CATGGCGTCT TTTCCCAGGC TGCATCTCCA GGGGTCACCT
    TTGCAACTTG CTGCTGCTCC CAGGGTCTCG TTTAGCTCCG GGTCTCAGCT GCCCAAAACC
    AATCAAGGGG GTGCCACAGG CTTCAGTGCC CTCACTACCC TTCAGCCAGA CCCTCTCTCA
    CGGCCGAATC TTTTTTCCTG TTCTGCAAGT CTTCTCCCTT GTCCACTTGT AAAGTCTGTC
    TTTTCTTCCC CTGCCTCTCC TCCCTCCCTA CCCAGGGGCG TTGTTCCCCC TCCTCCCCCT
    CTACCTGGGT GGTGTGGTGT TCTTCCTTCT CCCCCTCTTC CCCCTCTAAC TGGGGGACTT
    GTTCCCCCTC CTCCCCCTCT ATCTCAGAGG GTTGGCATTC CTCCTCCCCC TCCTCCCCCT
    CTACCTGGGT GGTGTGGTGT TCCTCCTTCT CCCCCACTTC CCCCTCTAAC TGGGGGACTT
    GTTCCCCCTC CTCCCCCTCT ACCTGGGTGG GGTGGTGTTC CTCCTCCTCC CCATCTTCCC
    CCTCTATCTG GAGGAATTGT TCCCCCTCCT CCCCCTCTAT CTCAGGGGGT TGGCATTCCT
    CCTCCCCCTC CTCCCCCTCT TTCCTCTCCT CTTCCCTCTC ACCATCTCCC ATCTCCTAGG
    AGTGCCAGTT ATAAGCTGCC AGTGGCTCCA TCTGGCCCTG CTGAGGATGC CCGGCTGGAC
    ATTCCTCTGT TAGCTGTGTA TGATAAGCCT TTGTCATTAG ACTCCCTCCA ATATGACTGT
    TCATTTTTTG AATGTTCTTC TGAAACGGAA AGTGATAAAA CAGGAGAACT TCTGGCAAGT
    AGGATTTTTA AAAAGCTGGC AGAATATGAC CTGAGAAGTG GCTTTACTGC AAGTACTCAG
    GCACAAAAAG ATGAGGATGG CCTAGTTGGC AAAGCAAGCT GGAGGAATTA TGTGCCTTCG
    ACTGAACTCT TCAGGCTTCA GACCAAGGAT GGATGCTGGA AACTTACACA AGAACTGGGA
    TTTATATTAA AACTTAATAC AAATATTTTA AACAGCTTTC TTGAGCAAAA AGGCATTCGA
    TCTTTAGGTG TAAGAGGAAG AGAGCGCCTT CTAGATCTGA TCGCCACGTT TCTAGTGCTG
    CAGTTCCTTC GCACCAAGTT GGAACAGGAG GGAATAATCG TCAAATCGCT GATGAGACTG
    GATGATGCTT CCATTCCCAG GAATATTCCC TGGGATTGCG AGAAAATAAA GAAAGCCAGT
    GAGTGGCTCA GAAGAACTGA AGGGCAGTAC CCATCCATCT GCCAGCGGCT GGAGCTGGGT
    AAAGACTGGG ACTCCGCCAC CAAGCAGCTG CTGGGTATCC TGCCCAGAGA TGCCGCCTCC
    CCTCCTCACA GGCTCCTTAA GCACAGCCAA GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002691914.3
    CDS150..5645
    Translation

    Target ORF information:

    RefSeq Version XM_002691868.6
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(ADP-ribose) polymerase family member 4 (PARP4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691868.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG GAATCTTCGC AAACTGTACC TTCTGTTTGA AAGCCAAGCA CTTAACTCGT 
    CAACAGAAGA GAAAACTACA AACTGATATT AAGGAAAATG GTGGAAATTT TTCCTTTTTA
    TTAAATGCTC AGTGCACACA TGTAATCTTA GACAATGCTG ATGTTCTGAG CCAGTATCAG
    CTGAAGTCTA TTCAGAAGAA CCATATTCTT ATTGCAAACC CAGATTTTGT ATGGGAATCT
    ATCAAAGAAA GAAGACTGCT GGATACAAAG ATTTATGGTC CCAGTGAGTC ACTGGACATC
    ACATCAGCTT CCAGTGAAGA ATGGAGCAGC CCTAAAATGG AAATGAATGA TTCTTCATTC
    CTGGACAGTT CCACAGAGAA GGAGAACACT GTTGAACTCA CTAAGTTTTA TGCAGAGGGT
    GTTGAAATTC CTCATTTTCC TCAAGATTTT GAAGTTGCAA AATATAACAT CTTGGAAAAA
    GTTGTGACAG AGGGAGGTAA GGAGACCGCC GTGGTGGAGC TGCAGTGCCT CCAGGGCCCT
    GGGGACCGTG GCTTTCTGAT ATGCACACAC TTCCTCCTGG CCACTGGCAC CCAGACCAGA
    AGGCAGTCCT CTGTGAAGAA AACCTCTGCA GACGCAAGTG AATACTATGA GAATTATATT
    GAGGAACTGA AGAAACAAGG CTTTCTACTG AGAGAACATT TTACACCTGA AGCAACCCAG
    TTAGCATCTG AAAAACTGCA AGCATTGCTT TTGGAGGAGG TAATAAACTC GAATGCCCTG
    AGCCCCGAGG TGAGCGGCTT GGTGGAGATG CTCTGGGCAG AAGCCCTCGG CCGCCTGGAG
    GACACACTGC TGAGACCTGT GGGCATGATG AGCCTGAATG ACGTGAGCAA GGCAGAGGGA
    ATTCTCCTCC TGGTAAAGGA AGCAATGAGA AATGGAGAAA CACAGGAGCA ACTGCAAACG
    ATGCTGACCG AGTTCTACAG ATTAATCCCT CACAGAGCTG CTGTGGCCGA GAAGGTCAGC
    CTGAGCCTGC TGGCTAAGAA GGAGGACCTG TGCCAGCTAA TAAGAGACAT GGTTAATGTC
    TGTGAAACTA ATTTGGCCAA ACCCAACCCC CCATCTCTTG CCAAATACCG AGCTTTGAGA
    TGCAAAATTG AGCATGTTGA GCCGAACACT GAAGAATTTT TGAGAGTAAG AGAAGAAGTG
    CTGCAGAATA ATCACAGTAA GGGTCCAGTG GACATCTTGC AGATATTTAG AGTTGGCAGA
    GTGAGTGAAA CCACAGAGTT CCAGGGCCAC CTTGGCAATG TCAGGCACTT ACTGCATGGG
    TCTCCTGTGC AGAGCTTCAT GGGAATCCTG TCCCGTGGGT TGCTTTTACC CAAAGTAGTT
    GAAGATCGTG GCATGAAGAG AACAGACATT GGAAATCTTG GGAGTGGGAT ATATTTCAGC
    GATTCAATCA GTACAAGCAT CAAGTACTCG CACCCTGGAC AGACAGATGG CGCCCGACTC
    CTGGTCGTCT GTGATGTCGC ACTCGGCAGG TGCATGGACC TGCACAAGCG AGACTTCTCC
    CTGACGGAGG CACCACCCGA GTACGACAGC GTCCACGGGG TGCGGGGAAC GGATATGGTC
    AGCTCGGACT TTGAGGATGA TGAATTTGTT GTATACAAAA CCAATCAGGT TAAAATGAAA
    TATCTTGTTA AATTTTCTGT ACCTGGAGAT GAAATAAAGG ACTTTCATCC TTGTGACAAT
    ACGGAACTAG AGGAAGACAG ACCTGAGTTT TCAAATTTTT CAGCAGTTGA AGATCACCAG
    CTTCCAGGCA GCACGCCTTT CCCCGACATC AAGGCCGGCC TCCAGGATGC CTCTGGGAAT
    TCAGTTCCTC TGGAGGACGT TCACATCAAG GGGAAAATAA TAGACTTTGT AGCCCAGGTC
    ATTGTTTTTC AAACATACAC AAACCAAAGT CATGTGCCCA TTGAGGCAAA ATATGTTTTT
    CCTTTGGACG ACAAGGCAGC TGTATGTGGT TTCGAGGCTT TCATCAATGG GAAGCACGTG
    GTGGGAGAGA TTAAAGAGAA GGAAGTGGCC CAGCGAGAGT ACCGAGTGGC GATCAGTCAA
    GGTCATGGCG CCTACCTGAT GGATCAGGAC ACACCCGACA TCTTCACCGT CAGCGTCGGG
    AACTTACCCC CAAAGGCCAA AGTCCTGGTC AAAATCACCT ACATCACGGA GCTGAGCATC
    CAGGGTGCCT GCGCGGTCTT CTTCCTGCCG GCCACGGTGG CGCCCTGGCA GCAGGACAGG
    GCTCTGAGGG AGAACCTTCA GGATACAGTG GAGAAGATTT GTATAAAGGA AATAGGAGCA
    AAGCAAAGCT TTTCTCTGAG CATGTCTATC GAGATGCCGC ACATGATTGA ATTCATCTCC
    AGTGACACAC ACGACCTGAG AAAAAAGAGA ACAGACTGCA AAGCTGTGAT TAGTACTGTG
    GAAGGCAGCT CCTTAGACAG CAATGGATTT TCTCTCCTCA TCGGTTTGGT TGATGTGTAT
    CTCCCAAGAA TGTGGGTTGA AAAGCATCCA GAAAAAGAAA GCGAGGCCTG CATGCTTGTG
    TTTCAACCAA ATCTTGGTAT TACCGTCCCT GACGAAGCTG AGAGGAGTGA AGTGATTATT
    TGTCTTGATT GCTCCAATTC CATGGAGGGC GAGACATTCC TGCAAGCCAA ACAGATTGCC
    TTGCATGCAC TGTCCTGTGT GGGAAAGGAG CAGAAGGTGA ACGTCATCAA GTTCGGCACA
    AGTTACAAGG AATTATTTTC ATATCCTAAG TGCATCACGA GCAATAAGAT GCCGACAGAG
    TTCATCATGT CTGCTACACC TACCATGGGA AACACAGATT TCTGGAAGAC ACTTCGATAC
    TTAAATTTGC TGTACCCTTC CCACGTGTTA CGGAACATCC TCCTCATATC CGACGGGCAC
    CTCCAGAACG AGAGCCTGAC CTTGCAGCTC GTAAGAAGAA GCGTGCCGCA CACCAGGCTG
    TTCTCCTGTG GGGTCAGTTC TACAGCAAAT CGTCATGTCT TACGGAGTCT GTCCCACTAT
    GGCGCTGGAG TATTTGAATA TTTTAACTCA AAATCCAAAC ATAGCTGGAA AAAACAGATA
    GAAGCCCAGA TGGATAGGCT GAGTTCTCCC AGTTGCCACT CGGTCTCCGT CAAATGGCAG
    CAGCTGAGCA CGGACACGCC CGCACCCCTG CAGGCCCCCA CCCAGGTGCA GTCCCTGTTC
    CACAGCGAGC GACTCCTTGT CTACGGGCTC ACTCCTCACT GTACACAGGC AACTCTACAT
    GCGCTGATTC AAGAGAGAGA GTATTCTACA GTGGTGTCAA CTACTGAACT TCAGAAGACA
    ACTGGAACGA TGATTCACAA GCTGGCAGCT CGAGCTCTGA TAAGAGATTA TGAAGATGGC
    ATTCTCCACG AAAACGAAGT GAATCACGAG ATGAAGAAGC AAACTTTAAA ATCTCTGATT
    ATTAAACTCA GTAAAGAAAA CTCTCTCATA ACACAGTTTA CAAGCTTTGT GGCTGTGGAG
    AAAAGGTATG GTAATGAGTC ACCTTCTCCT GATATTCCAA ATACTTTGGA ACTTATTGCC
    AAAGAAGACA TAGATTTTCT GCCATACATG AGCTGGGAGG GGGAACAACC AGATGCCAGC
    AGGATGCAGA CACTTTCAGC ATCGTCTGAA TGGAACCAAG AACCCAAACG TAAATGTAAA
    CGTAAACAGA CTAAAAAGAA GCGTTCATCA CTTAAGATGA TACTTTCTGA AGATTTTAAA
    GCCTTTAGCT CAGATGCCCC AGAAGAATCT GAAGCGGTCA GTTTTGTCTT CAGGGATGCA
    GAGGAGCCCT GGGGTTTGGG TCTGATGGAG TCATCTAAAC AAACAGAAGT TAAACCACAA
    TTCACAGAAG CTATCTCACA GAAAACGGCC TTTTCTGACA TTCCTACTGC CCCTAGTTTC
    TTAACTACAC CCCTCCATGT TGGCTCTGGT TTCTACCTTC AGGCCAGTTC AGCACTGTTT
    GGTTCTCCAG CTGGTCCCAA ACACTTCGGT TCCCCTAAGA AGTACCTGGA AATGGGCAGT
    GGTGCCGACG TTTCCCTGGA CATGGCGTCT TTTCCCAGGC TGCATCTCCA GGGGTCACCT
    TTGCAACTTG CTGCTGCTCC CAGGGTCTCG TTTAGCTCCG GGTCTCAGCT GCCCAAAACC
    AATCAAGGGG GTGCCACAGG CTTCAGTGCC CTCACTACCC TTCAGCCAGA CCCTCTCTCA
    CGGCCGAATC TTTTTTCCTG TTCTGCAAGT CTTCTCCCTT GTCCACTTGT AAAGTCTGTC
    TTTTCTTCCC CTGCCTCTCC TCCCTCCCTA CCCAGGGGCG TTGTTCCCCC TCCTCCCCCT
    CTACCTGGGT GGTGTGGTGT TCTTCCTTCT CCCCCTCTTC CCCCTCTAAC TGGGGGACTT
    GTTCCCCCTC CTCCCCCTCT ATCTCAGAGG GTTGGCATTC CTCCTCCCCC TCCTCCCCCT
    CTACCTGGGT GGTGTGGTGT TCCTCCTTCT CCCCCACTTC CCCCTCTAAC TGGGGGACTT
    GTTCCCCCTC CTCCCCCTCT ACCTGGGTGG GGTGGTGTTC CTCCTCCTCC CCATCTTCCC
    CCTCTATCTG GAGGAATTGT TCCCCCTCCT CCCCCTCTAT CTCAGGGGGT TGGCATTCCT
    CCTCCCCCTC CTCCCCCTCT TTCCTCTCCT CTTCCCTCTC ACCATCTCCC ATCTCCTAGG
    AGTGCCAGTT ATAAGCTGCC AGTGGCTCCA TCTGGCCCTG CTGAGGATGC CCGGCTGGAC
    ATTCCTCTGT TAGCTGTGTA TGATAAGCCT TTGTCATTAG ACTCCCTCCA ATATGACTGT
    TCATTTTTTG AATGTTCTTC TGAAACGGAA AGTGATAAAA CAGGAGAACT TCTGGCAAGT
    AGGATTTTTA AAAAGCTGGC AGAATATGAC CTGAGAAGTG GCTTTACTGC AAGTACTCAG
    GCACAAAAAG ATGAGGATGG CCTAGTTGGC AAAGCAAGCT GGAGGAATTA TGTGCCTTCG
    ACTGAACTCT TCAGGCTTCA GACCAAGGAT GGATGCTGGA AACTTACACA AGAACTGGGA
    TTTATATTAA AACTTAATAC AAATATTTTA AACAGCTTTC TTGAGCAAAA AGGCATTCGA
    TCTTTAGGTG TAAGAGGAAG AGAGCGCCTT CTAGATCTGA TCGCCACGTT TCTAGTGCTG
    CAGTTCCTTC GCACCAAGTT GGAACAGGAG GGAATAATCG TCAAATCGCT GATGAGACTG
    GATGATGCTT CCATTCCCAG GAATATTCCC TGGGATTGCG AGAAAATAAA GAAAGCCAGT
    GAGTGGCTCA GAAGAACTGA AGGGCAGTAC CCATCCATCT GCCAGCGGCT GGAGCTGGGT
    AAAGACTGGG ACTCCGCCAC CAAGCAGCTG CTGGGTATCC TGCCCAGAGA TGCCGCCTCC
    CCTCCTCACA GGCTCCTTAA GCACAGCCAA GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24788
    Clone ID Related Accession (Same CDS sequence) XM_867149.8 , XM_002691868.5
    Accession Version XM_867149.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product poly [ADP-ribose] polymerase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645955.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_867149.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG GAATCTTCGC AAACTGTACC TTCTGTTTGA AAGCCAAGCA CTTAACTCGT 
    CAACAGAAGA GAAAACTACA AACTGATATT AAGGAAAATG GTGGAAATTT TTCCTTTTTA
    TTAAATGCTC AGTGCACACA TGTAATCTTA GACAATGCTG ATGTTCTGAG CCAGTATCAG
    CTGAAGTCTA TTCAGAAGAA CCATATTCTT ATTGCAAACC CAGATTTTGT ATGGGAATCT
    ATCAAAGAAA GAAGACTGCT GGATACAAAG ATTTATGGTC CCAGTGAGTC ACTGGACATC
    ACATCAGCTT CCAGTGAAGA ATGGAGCAGC CCTAAAATGG AAATGAATGA TTCTTCATTC
    CTGGACAGTT CCACAGAGAA GGAGAACACT GTTGAACTCA CTAAGTTTTA TGCAGAGGGT
    GTTGAAATTC CTCATTTTCC TCAAGATTTT GAAGTTGCAA AATATAACAT CTTGGAAAAA
    GTTGTGACAG AGGGAGGTAA GGAGACCGCC GTGGTGGAGC TGCAGTGCCT CCAGGGCCCT
    GGGGACCGTG GCTTTCTGAT ATGCACACAC TTCCTCCTGG CCACTGGCAC CCAGACCAGA
    AGGCAGTCCT CTGTGAAGAA AACCTCTGCA GACGCAAGTG AATACTATGA GAATTATATT
    GAGGAACTGA AGAAACAAGG CTTTCTACTG AGAGAACATT TTACACCTGA AGCAACCCAG
    TTAGCATCTG AAAAACTGCA AGCATTGCTT TTGGAGGAGG TAATAAACTC GAATGCCCTG
    AGCCCCGAGG TGAGCGGCTT GGTGGAGATG CTCTGGGCAG AAGCCCTCGG CCGCCTGGAG
    GACACACTGC TGAGACCTGT GGGCATGATG AGCCTGAATG ACGTGAGCAA GGCAGAGGGA
    ATTCTCCTCC TGGTAAAGGA AGCAATGAGA AATGGAGAAA CACAGGAGCA ACTGCAAACG
    ATGCTGACCG AGTTCTACAG ATTAATCCCT CACAGAGCTG CTGTGGCCGA GAAGGTCAGC
    CTGAGCCTGC TGGCTAAGAA GGAGGACCTG TGCCAGCTAA TAAGAGACAT GGTTAATGTC
    TGTGAAACTA ATTTGGCCAA ACCCAACCCC CCATCTCTTG CCAAATACCG AGCTTTGAGA
    TGCAAAATTG AGCATGTTGA GCCGAACACT GAAGAATTTT TGAGAGTAAG AGAAGAAGTG
    CTGCAGAATA ATCACAGTAA GGGTCCAGTG GACATCTTGC AGATATTTAG AGTTGGCAGA
    GTGAGTGAAA CCACAGAGTT CCAGGGCCAC CTTGGCAATG TCAGGCACTT ACTGCATGGG
    TCTCCTGTGC AGAGCTTCAT GGGAATCCTG TCCCGTGGGT TGCTTTTACC CAAAGTAGTT
    GAAGATCGTG GCATGAAGAG AACAGACATT GGAAATCTTG GGAGTGGGAT ATATTTCAGC
    GATTCAATCA GTACAAGCAT CAAGTACTCG CACCCTGGAC AGACAGATGG CGCCCGACTC
    CTGGTCGTCT GTGATGTCGC ACTCGGCAGG TGCATGGACC TGCACAAGCG AGACTTCTCC
    CTGACGGAGG CACCACCCGA GTACGACAGC GTCCACGGGG TGCGGGGAAC GGATATGGTC
    AGCTCGGACT TTGAGGATGA TGAATTTGTT GTATACAAAA CCAATCAGGT TAAAATGAAA
    TATCTTGTTA AATTTTCTGT ACCTGGAGAT GAAATAAAGG ACTTTCATCC TTGTGACAAT
    ACGGAACTAG AGGAAGACAG ACCTGAGTTT TCAAATTTTT CAGCAGTTGA AGATCACCAG
    CTTCCAGGCA GCACGCCTTT CCCCGACATC AAGGCCGGCC TCCAGGATGC CTCTGGGAAT
    TCAGTTCCTC TGGAGGACGT TCACATCAAG GGGAAAATAA TAGACTTTGT AGCCCAGGTC
    ATTGTTTTTC AAACATACAC AAACCAAAGT CATGTGCCCA TTGAGGCAAA ATATGTTTTT
    CCTTTGGACG ACAAGGCAGC TGTATGTGGT TTCGAGGCTT TCATCAATGG GAAGCACGTG
    GTGGGAGAGA TTAAAGAGAA GGAAGTGGCC CAGCGAGAGT ACCGAGTGGC GATCAGTCAA
    GGTCATGGCG CCTACCTGAT GGATCAGGAC ACACCCGACA TCTTCACCGT CAGCGTCGGG
    AACTTACCCC CAAAGGCCAA AGTCCTGGTC AAAATCACCT ACATCACGGA GCTGAGCATC
    CAGGGTGCCT GCGCGGTCTT CTTCCTGCCG GCCACGGTGG CGCCCTGGCA GCAGGACAGG
    GCTCTGAGGG AGAACCTTCA GGATACAGTG GAGAAGATTT GTATAAAGGA AATAGGAGCA
    AAGCAAAGCT TTTCTCTGAG CATGTCTATC GAGATGCCGC ACATGATTGA ATTCATCTCC
    AGTGACACAC ACGACCTGAG AAAAAAGAGA ACAGACTGCA AAGCTGTGAT TAGTACTGTG
    GAAGGCAGCT CCTTAGACAG CAATGGATTT TCTCTCCTCA TCGGTTTGGT TGATGTGTAT
    CTCCCAAGAA TGTGGGTTGA AAAGCATCCA GAAAAAGAAA GCGAGGCCTG CATGCTTGTG
    TTTCAACCAA ATCTTGGTAT TACCGTCCCT GACGAAGCTG AGAGGAGTGA AGTGATTATT
    TGTCTTGATT GCTCCAATTC CATGGAGGGC GAGACATTCC TGCAAGCCAA ACAGATTGCC
    TTGCATGCAC TGTCCTGTGT GGGAAAGGAG CAGAAGGTGA ACGTCATCAA GTTCGGCACA
    AGTTACAAGG AATTATTTTC ATATCCTAAG TGCATCACGA GCAATAAGAT GCCGACAGAG
    TTCATCATGT CTGCTACACC TACCATGGGA AACACAGATT TCTGGAAGAC ACTTCGATAC
    TTAAATTTGC TGTACCCTTC CCACGTGTTA CGGAACATCC TCCTCATATC CGACGGGCAC
    CTCCAGAACG AGAGCCTGAC CTTGCAGCTC GTAAGAAGAA GCGTGCCGCA CACCAGGCTG
    TTCTCCTGTG GGGTCAGTTC TACAGCAAAT CGTCATGTCT TACGGAGTCT GTCCCACTAT
    GGCGCTGGAG TATTTGAATA TTTTAACTCA AAATCCAAAC ATAGCTGGAA AAAACAGATA
    GAAGCCCAGA TGGATAGGCT GAGTTCTCCC AGTTGCCACT CGGTCTCCGT CAAATGGCAG
    CAGCTGAGCA CGGACACGCC CGCACCCCTG CAGGCCCCCA CCCAGGTGCA GTCCCTGTTC
    CACAGCGAGC GACTCCTTGT CTACGGGCTC ACTCCTCACT GTACACAGGC AACTCTACAT
    GCGCTGATTC AAGAGAGAGA GTATTCTACA GTGGTGTCAA CTACTGAACT TCAGAAGACA
    ACTGGAACGA TGATTCACAA GCTGGCAGCT CGAGCTCTGA TAAGAGATTA TGAAGATGGC
    ATTCTCCACG AAAACGAAGT GAATCACGAG ATGAAGAAGC AAACTTTAAA ATCTCTGATT
    ATTAAACTCA GTAAAGAAAA CTCTCTCATA ACACAGTTTA CAAGCTTTGT GGCTGTGGAG
    AAAAGGTATG GTAATGAGTC ACCTTCTCCT GATATTCCAA ATACTTTGGA ACTTATTGCC
    AAAGAAGACA TAGATTTTCT GCCATACATG AGCTGGGAGG GGGAACAACC AGATGCCAGC
    AGGATGCAGA CACTTTCAGC ATCGTCTGAA TGGAACCAAG AACCCAAACG TAAATGTAAA
    CGTAAACAGA CTAAAAAGAA GCGTTCATCA CTTAAGATGA TACTTTCTGA AGATTTTAAA
    GCCTTTAGCT CAGATGCCCC AGAAGAATCT GAAGCGGTCA GTTTTGTCTT CAGGGATGCA
    GAGGAGCCCT GGGGTTTGGG TCTGATGGAG TCATCTAAAC AAACAGAAGT TAAACCACAA
    TTCACAGAAG CTATCTCACA GAAAACGGCC TTTTCTGACA TTCCTACTGC CCCTAGTTTC
    TTAACTACAC CCCTCCATGT TGGCTCTGGT TTCTACCTTC AGGCCAGTTC AGCACTGTTT
    GGTTCTCCAG CTGGTCCCAA ACACTTCGGT TCCCCTAAGA AGTACCTGGA AATGGGCAGT
    GGTGCCGACG TTTCCCTGGA CATGGCGTCT TTTCCCAGGC TGCATCTCCA GGGGTCACCT
    TTGCAACTTG CTGCTGCTCC CAGGGTCTCG TTTAGCTCCG GGTCTCAGCT GCCCAAAACC
    AATCAAGGGG GTGCCACAGG CTTCAGTGCC CTCACTACCC TTCAGCCAGA CCCTCTCTCA
    CGGCCGAATC TTTTTTCCTG TTCTGCAAGT CTTCTCCCTT GTCCACTTGT AAAGTCTGTC
    TTTTCTTCCC CTGCCTCTCC TCCCTCCCTA CCCAGGGGCG TTGTTCCCCC TCCTCCCCCT
    CTACCTGGGT GGTGTGGTGT TCTTCCTTCT CCCCCTCTTC CCCCTCTAAC TGGGGGACTT
    GTTCCCCCTC CTCCCCCTCT ATCTCAGAGG GTTGGCATTC CTCCTCCCCC TCCTCCCCCT
    CTACCTGGGT GGTGTGGTGT TCCTCCTTCT CCCCCACTTC CCCCTCTAAC TGGGGGACTT
    GTTCCCCCTC CTCCCCCTCT ACCTGGGTGG GGTGGTGTTC CTCCTCCTCC CCATCTTCCC
    CCTCTATCTC AGGGGGTGGG CATTCCTCCT CCCCCTCCTC CCCCTCTTTC CTCTCCTCTT
    CCCTCTCACC ATCTCCCATC TCCTAGGAGT GCCAGTTATA AGCTGCCAGT GGCTCCATCT
    GGCCCTGCTG AGGATGCCCG GCTGGACATT CCTCTGTTAG CTGTGTATGA TAAGCCTTTG
    TCATTAGACT CCCTCCAATA TGACTGTTCA TTTTTTGAAT GTTCTTCTGA AACGGAAAGT
    GATAAAACAG GAGAACTTCT GGCAAGTAGG ATTTTTAAAA AGCTGGCAGA ATATGACCTG
    AGAAGTGGCT TTACTGCAAG TACTCAGGCA CAAAAAGATG AGGATGGCCT AGTTGGCAAA
    GCAAGCTGGA GGAATTATGT GCCTTCGACT GAACTCTTCA GGCTTCAGAC CAAGGATGGA
    TGCTGGAAAC TTACACAAGA ACTGGGATTT ATATTAAAAC TTAATACAAA TATTTTAAAC
    AGCTTTCTTG AGCAAAAAGG CATTCGATCT TTAGGTGTAA GAGGAAGAGA GCGCCTTCTA
    GATCTGATCG CCACGTTTCT AGTGCTGCAG TTCCTTCGCA CCAAGTTGGA ACAGGAGGGA
    ATAATCGTCA AATCGCTGAT GAGACTGGAT GATGCTTCCA TTCCCAGGAA TATTCCCTGG
    GATTGCGAGA AAATAAAGAA AGCCAGTGAG TGGCTCAGAA GAACTGAAGG GCAGTACCCA
    TCCATCTGCC AGCGGCTGGA GCTGGGTAAA GACTGGGACT CCGCCACCAA GCAGCTGCTG
    GGTATCCTGC CCAGAGATGC CGCCTCCCCT CCTCACAGGC TCCTTAAGCA CAGCCAAGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_872242.7
    CDS133..5595
    Translation

    Target ORF information:

    RefSeq Version XM_867149.8
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(ADP-ribose) polymerase family member 4 (PARP4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_867149.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG GAATCTTCGC AAACTGTACC TTCTGTTTGA AAGCCAAGCA CTTAACTCGT 
    CAACAGAAGA GAAAACTACA AACTGATATT AAGGAAAATG GTGGAAATTT TTCCTTTTTA
    TTAAATGCTC AGTGCACACA TGTAATCTTA GACAATGCTG ATGTTCTGAG CCAGTATCAG
    CTGAAGTCTA TTCAGAAGAA CCATATTCTT ATTGCAAACC CAGATTTTGT ATGGGAATCT
    ATCAAAGAAA GAAGACTGCT GGATACAAAG ATTTATGGTC CCAGTGAGTC ACTGGACATC
    ACATCAGCTT CCAGTGAAGA ATGGAGCAGC CCTAAAATGG AAATGAATGA TTCTTCATTC
    CTGGACAGTT CCACAGAGAA GGAGAACACT GTTGAACTCA CTAAGTTTTA TGCAGAGGGT
    GTTGAAATTC CTCATTTTCC TCAAGATTTT GAAGTTGCAA AATATAACAT CTTGGAAAAA
    GTTGTGACAG AGGGAGGTAA GGAGACCGCC GTGGTGGAGC TGCAGTGCCT CCAGGGCCCT
    GGGGACCGTG GCTTTCTGAT ATGCACACAC TTCCTCCTGG CCACTGGCAC CCAGACCAGA
    AGGCAGTCCT CTGTGAAGAA AACCTCTGCA GACGCAAGTG AATACTATGA GAATTATATT
    GAGGAACTGA AGAAACAAGG CTTTCTACTG AGAGAACATT TTACACCTGA AGCAACCCAG
    TTAGCATCTG AAAAACTGCA AGCATTGCTT TTGGAGGAGG TAATAAACTC GAATGCCCTG
    AGCCCCGAGG TGAGCGGCTT GGTGGAGATG CTCTGGGCAG AAGCCCTCGG CCGCCTGGAG
    GACACACTGC TGAGACCTGT GGGCATGATG AGCCTGAATG ACGTGAGCAA GGCAGAGGGA
    ATTCTCCTCC TGGTAAAGGA AGCAATGAGA AATGGAGAAA CACAGGAGCA ACTGCAAACG
    ATGCTGACCG AGTTCTACAG ATTAATCCCT CACAGAGCTG CTGTGGCCGA GAAGGTCAGC
    CTGAGCCTGC TGGCTAAGAA GGAGGACCTG TGCCAGCTAA TAAGAGACAT GGTTAATGTC
    TGTGAAACTA ATTTGGCCAA ACCCAACCCC CCATCTCTTG CCAAATACCG AGCTTTGAGA
    TGCAAAATTG AGCATGTTGA GCCGAACACT GAAGAATTTT TGAGAGTAAG AGAAGAAGTG
    CTGCAGAATA ATCACAGTAA GGGTCCAGTG GACATCTTGC AGATATTTAG AGTTGGCAGA
    GTGAGTGAAA CCACAGAGTT CCAGGGCCAC CTTGGCAATG TCAGGCACTT ACTGCATGGG
    TCTCCTGTGC AGAGCTTCAT GGGAATCCTG TCCCGTGGGT TGCTTTTACC CAAAGTAGTT
    GAAGATCGTG GCATGAAGAG AACAGACATT GGAAATCTTG GGAGTGGGAT ATATTTCAGC
    GATTCAATCA GTACAAGCAT CAAGTACTCG CACCCTGGAC AGACAGATGG CGCCCGACTC
    CTGGTCGTCT GTGATGTCGC ACTCGGCAGG TGCATGGACC TGCACAAGCG AGACTTCTCC
    CTGACGGAGG CACCACCCGA GTACGACAGC GTCCACGGGG TGCGGGGAAC GGATATGGTC
    AGCTCGGACT TTGAGGATGA TGAATTTGTT GTATACAAAA CCAATCAGGT TAAAATGAAA
    TATCTTGTTA AATTTTCTGT ACCTGGAGAT GAAATAAAGG ACTTTCATCC TTGTGACAAT
    ACGGAACTAG AGGAAGACAG ACCTGAGTTT TCAAATTTTT CAGCAGTTGA AGATCACCAG
    CTTCCAGGCA GCACGCCTTT CCCCGACATC AAGGCCGGCC TCCAGGATGC CTCTGGGAAT
    TCAGTTCCTC TGGAGGACGT TCACATCAAG GGGAAAATAA TAGACTTTGT AGCCCAGGTC
    ATTGTTTTTC AAACATACAC AAACCAAAGT CATGTGCCCA TTGAGGCAAA ATATGTTTTT
    CCTTTGGACG ACAAGGCAGC TGTATGTGGT TTCGAGGCTT TCATCAATGG GAAGCACGTG
    GTGGGAGAGA TTAAAGAGAA GGAAGTGGCC CAGCGAGAGT ACCGAGTGGC GATCAGTCAA
    GGTCATGGCG CCTACCTGAT GGATCAGGAC ACACCCGACA TCTTCACCGT CAGCGTCGGG
    AACTTACCCC CAAAGGCCAA AGTCCTGGTC AAAATCACCT ACATCACGGA GCTGAGCATC
    CAGGGTGCCT GCGCGGTCTT CTTCCTGCCG GCCACGGTGG CGCCCTGGCA GCAGGACAGG
    GCTCTGAGGG AGAACCTTCA GGATACAGTG GAGAAGATTT GTATAAAGGA AATAGGAGCA
    AAGCAAAGCT TTTCTCTGAG CATGTCTATC GAGATGCCGC ACATGATTGA ATTCATCTCC
    AGTGACACAC ACGACCTGAG AAAAAAGAGA ACAGACTGCA AAGCTGTGAT TAGTACTGTG
    GAAGGCAGCT CCTTAGACAG CAATGGATTT TCTCTCCTCA TCGGTTTGGT TGATGTGTAT
    CTCCCAAGAA TGTGGGTTGA AAAGCATCCA GAAAAAGAAA GCGAGGCCTG CATGCTTGTG
    TTTCAACCAA ATCTTGGTAT TACCGTCCCT GACGAAGCTG AGAGGAGTGA AGTGATTATT
    TGTCTTGATT GCTCCAATTC CATGGAGGGC GAGACATTCC TGCAAGCCAA ACAGATTGCC
    TTGCATGCAC TGTCCTGTGT GGGAAAGGAG CAGAAGGTGA ACGTCATCAA GTTCGGCACA
    AGTTACAAGG AATTATTTTC ATATCCTAAG TGCATCACGA GCAATAAGAT GCCGACAGAG
    TTCATCATGT CTGCTACACC TACCATGGGA AACACAGATT TCTGGAAGAC ACTTCGATAC
    TTAAATTTGC TGTACCCTTC CCACGTGTTA CGGAACATCC TCCTCATATC CGACGGGCAC
    CTCCAGAACG AGAGCCTGAC CTTGCAGCTC GTAAGAAGAA GCGTGCCGCA CACCAGGCTG
    TTCTCCTGTG GGGTCAGTTC TACAGCAAAT CGTCATGTCT TACGGAGTCT GTCCCACTAT
    GGCGCTGGAG TATTTGAATA TTTTAACTCA AAATCCAAAC ATAGCTGGAA AAAACAGATA
    GAAGCCCAGA TGGATAGGCT GAGTTCTCCC AGTTGCCACT CGGTCTCCGT CAAATGGCAG
    CAGCTGAGCA CGGACACGCC CGCACCCCTG CAGGCCCCCA CCCAGGTGCA GTCCCTGTTC
    CACAGCGAGC GACTCCTTGT CTACGGGCTC ACTCCTCACT GTACACAGGC AACTCTACAT
    GCGCTGATTC AAGAGAGAGA GTATTCTACA GTGGTGTCAA CTACTGAACT TCAGAAGACA
    ACTGGAACGA TGATTCACAA GCTGGCAGCT CGAGCTCTGA TAAGAGATTA TGAAGATGGC
    ATTCTCCACG AAAACGAAGT GAATCACGAG ATGAAGAAGC AAACTTTAAA ATCTCTGATT
    ATTAAACTCA GTAAAGAAAA CTCTCTCATA ACACAGTTTA CAAGCTTTGT GGCTGTGGAG
    AAAAGGTATG GTAATGAGTC ACCTTCTCCT GATATTCCAA ATACTTTGGA ACTTATTGCC
    AAAGAAGACA TAGATTTTCT GCCATACATG AGCTGGGAGG GGGAACAACC AGATGCCAGC
    AGGATGCAGA CACTTTCAGC ATCGTCTGAA TGGAACCAAG AACCCAAACG TAAATGTAAA
    CGTAAACAGA CTAAAAAGAA GCGTTCATCA CTTAAGATGA TACTTTCTGA AGATTTTAAA
    GCCTTTAGCT CAGATGCCCC AGAAGAATCT GAAGCGGTCA GTTTTGTCTT CAGGGATGCA
    GAGGAGCCCT GGGGTTTGGG TCTGATGGAG TCATCTAAAC AAACAGAAGT TAAACCACAA
    TTCACAGAAG CTATCTCACA GAAAACGGCC TTTTCTGACA TTCCTACTGC CCCTAGTTTC
    TTAACTACAC CCCTCCATGT TGGCTCTGGT TTCTACCTTC AGGCCAGTTC AGCACTGTTT
    GGTTCTCCAG CTGGTCCCAA ACACTTCGGT TCCCCTAAGA AGTACCTGGA AATGGGCAGT
    GGTGCCGACG TTTCCCTGGA CATGGCGTCT TTTCCCAGGC TGCATCTCCA GGGGTCACCT
    TTGCAACTTG CTGCTGCTCC CAGGGTCTCG TTTAGCTCCG GGTCTCAGCT GCCCAAAACC
    AATCAAGGGG GTGCCACAGG CTTCAGTGCC CTCACTACCC TTCAGCCAGA CCCTCTCTCA
    CGGCCGAATC TTTTTTCCTG TTCTGCAAGT CTTCTCCCTT GTCCACTTGT AAAGTCTGTC
    TTTTCTTCCC CTGCCTCTCC TCCCTCCCTA CCCAGGGGCG TTGTTCCCCC TCCTCCCCCT
    CTACCTGGGT GGTGTGGTGT TCTTCCTTCT CCCCCTCTTC CCCCTCTAAC TGGGGGACTT
    GTTCCCCCTC CTCCCCCTCT ATCTCAGAGG GTTGGCATTC CTCCTCCCCC TCCTCCCCCT
    CTACCTGGGT GGTGTGGTGT TCCTCCTTCT CCCCCACTTC CCCCTCTAAC TGGGGGACTT
    GTTCCCCCTC CTCCCCCTCT ACCTGGGTGG GGTGGTGTTC CTCCTCCTCC CCATCTTCCC
    CCTCTATCTC AGGGGGTGGG CATTCCTCCT CCCCCTCCTC CCCCTCTTTC CTCTCCTCTT
    CCCTCTCACC ATCTCCCATC TCCTAGGAGT GCCAGTTATA AGCTGCCAGT GGCTCCATCT
    GGCCCTGCTG AGGATGCCCG GCTGGACATT CCTCTGTTAG CTGTGTATGA TAAGCCTTTG
    TCATTAGACT CCCTCCAATA TGACTGTTCA TTTTTTGAAT GTTCTTCTGA AACGGAAAGT
    GATAAAACAG GAGAACTTCT GGCAAGTAGG ATTTTTAAAA AGCTGGCAGA ATATGACCTG
    AGAAGTGGCT TTACTGCAAG TACTCAGGCA CAAAAAGATG AGGATGGCCT AGTTGGCAAA
    GCAAGCTGGA GGAATTATGT GCCTTCGACT GAACTCTTCA GGCTTCAGAC CAAGGATGGA
    TGCTGGAAAC TTACACAAGA ACTGGGATTT ATATTAAAAC TTAATACAAA TATTTTAAAC
    AGCTTTCTTG AGCAAAAAGG CATTCGATCT TTAGGTGTAA GAGGAAGAGA GCGCCTTCTA
    GATCTGATCG CCACGTTTCT AGTGCTGCAG TTCCTTCGCA CCAAGTTGGA ACAGGAGGGA
    ATAATCGTCA AATCGCTGAT GAGACTGGAT GATGCTTCCA TTCCCAGGAA TATTCCCTGG
    GATTGCGAGA AAATAAAGAA AGCCAGTGAG TGGCTCAGAA GAACTGAAGG GCAGTACCCA
    TCCATCTGCC AGCGGCTGGA GCTGGGTAAA GACTGGGACT CCGCCACCAA GCAGCTGCTG
    GGTATCCTGC CCAGAGATGC CGCCTCCCCT CCTCACAGGC TCCTTAAGCA CAGCCAAGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24788
    Clone ID Related Accession (Same CDS sequence) XM_867149.8 , XM_002691868.5
    Accession Version XM_002691868.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product poly [ADP-ribose] polymerase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104312.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691868.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG GAATCTTCGC AAACTGTACC TTCTGTTTGA AAGCCAAGCA CTTAACTCGT 
    CAACAGAAGA GAAAACTACA AACTGATATT AAGGAAAATG GTGGAAATTT TTCCTTTTTA
    TTAAATGCTC AGTGCACACA TGTAATCTTA GACAATGCTG ATGTTCTGAG CCAGTATCAG
    CTGAAGTCTA TTCAGAAGAA CCATATTCTT ATTGCAAACC CAGATTTTGT ATGGGAATCT
    ATCAAAGAAA GAAGACTGCT GGATACAAAG ATTTATGGTC CCAGTGAGTC ACTGGACATC
    ACATCAGCTT CCAGTGAAGA ATGGAGCAGC CCTAAAATGG AAATGAATGA TTCTTCATTC
    CTGGACAGTT CCACAGAGAA GGAGAACACT GTTGAACTCA CTAAGTTTTA TGCAGAGGGT
    GTTGAAATTC CTCATTTTCC TCAAGATTTT GAAGTTGCAA AATATAACAT CTTGGAAAAA
    GTTGTGACAG AGGGAGGTAA GGAGACCGCC GTGGTGGAGC TGCAGTGCCT CCAGGGCCCT
    GGGGACCGTG GCTTTCTGAT ATGCACACAC TTCCTCCTGG CCACTGGCAC CCAGACCAGA
    AGGCAGTCCT CTGTGAAGAA AACCTCTGCA GACGCAAGTG AATACTATGA GAATTATATT
    GAGGAACTGA AGAAACAAGG CTTTCTACTG AGAGAACATT TTACACCTGA AGCAACCCAG
    TTAGCATCTG AAAAACTGCA AGCATTGCTT TTGGAGGAGG TAATAAACTC GAATGCCCTG
    AGCCCCGAGG TGAGCGGCTT GGTGGAGATG CTCTGGGCAG AAGCCCTCGG CCGCCTGGAG
    GACACACTGC TGAGACCTGT GGGCATGATG AGCCTGAATG ACGTGAGCAA GGCAGAGGGA
    ATTCTCCTCC TGGTAAAGGA AGCAATGAGA AATGGAGAAA CACAGGAGCA ACTGCAAACG
    ATGCTGACCG AGTTCTACAG ATTAATCCCT CACAGAGCTG CTGTGGCCGA GAAGGTCAGC
    CTGAGCCTGC TGGCTAAGAA GGAGGACCTG TGCCAGCTAA TAAGAGACAT GGTTAATGTC
    TGTGAAACTA ATTTGGCCAA ACCCAACCCC CCATCTCTTG CCAAATACCG AGCTTTGAGA
    TGCAAAATTG AGCATGTTGA GCCGAACACT GAAGAATTTT TGAGAGTAAG AGAAGAAGTG
    CTGCAGAATA ATCACAGTAA GGGTCCAGTG GACATCTTGC AGATATTTAG AGTTGGCAGA
    GTGAGTGAAA CCACAGAGTT CCAGGGCCAC CTTGGCAATG TCAGGCACTT ACTGCATGGG
    TCTCCTGTGC AGAGCTTCAT GGGAATCCTG TCCCGTGGGT TGCTTTTACC CAAAGTAGTT
    GAAGATCGTG GCATGAAGAG AACAGACATT GGAAATCTTG GGAGTGGGAT ATATTTCAGC
    GATTCAATCA GTACAAGCAT CAAGTACTCG CACCCTGGAC AGACAGATGG CGCCCGACTC
    CTGGTCGTCT GTGATGTCGC ACTCGGCAGG TGCATGGACC TGCACAAGCG AGACTTCTCC
    CTGACGGAGG CACCACCCGA GTACGACAGC GTCCACGGGG TGCGGGGAAC GGATATGGTC
    AGCTCGGACT TTGAGGATGA TGAATTTGTT GTATACAAAA CCAATCAGGT TAAAATGAAA
    TATCTTGTTA AATTTTCTGT ACCTGGAGAT GAAATAAAGG ACTTTCATCC TTGTGACAAT
    ACGGAACTAG AGGAAGACAG ACCTGAGTTT TCAAATTTTT CAGCAGTTGA AGATCACCAG
    CTTCCAGGCA GCACGCCTTT CCCCGACATC AAGGCCGGCC TCCAGGATGC CTCTGGGAAT
    TCAGTTCCTC TGGAGGACGT TCACATCAAG GGGAAAATAA TAGACTTTGT AGCCCAGGTC
    ATTGTTTTTC AAACATACAC AAACCAAAGT CATGTGCCCA TTGAGGCAAA ATATGTTTTT
    CCTTTGGACG ACAAGGCAGC TGTATGTGGT TTCGAGGCTT TCATCAATGG GAAGCACGTG
    GTGGGAGAGA TTAAAGAGAA GGAAGTGGCC CAGCGAGAGT ACCGAGTGGC GATCAGTCAA
    GGTCATGGCG CCTACCTGAT GGATCAGGAC ACACCCGACA TCTTCACCGT CAGCGTCGGG
    AACTTACCCC CAAAGGCCAA AGTCCTGGTC AAAATCACCT ACATCACGGA GCTGAGCATC
    CAGGGTGCCT GCGCGGTCTT CTTCCTGCCG GCCACGGTGG CGCCCTGGCA GCAGGACAGG
    GCTCTGAGGG AGAACCTTCA GGATACAGTG GAGAAGATTT GTATAAAGGA AATAGGAGCA
    AAGCAAAGCT TTTCTCTGAG CATGTCTATC GAGATGCCGC ACATGATTGA ATTCATCTCC
    AGTGACACAC ACGACCTGAG AAAAAAGAGA ACAGACTGCA AAGCTGTGAT TAGTACTGTG
    GAAGGCAGCT CCTTAGACAG CAATGGATTT TCTCTCCTCA TCGGTTTGGT TGATGTGTAT
    CTCCCAAGAA TGTGGGTTGA AAAGCATCCA GAAAAAGAAA GCGAGGCCTG CATGCTTGTG
    TTTCAACCAA ATCTTGGTAT TACCGTCCCT GACGAAGCTG AGAGGAGTGA AGTGATTATT
    TGTCTTGATT GCTCCAATTC CATGGAGGGC GAGACATTCC TGCAAGCCAA ACAGATTGCC
    TTGCATGCAC TGTCCTGTGT GGGAAAGGAG CAGAAGGTGA ACGTCATCAA GTTCGGCACA
    AGTTACAAGG AATTATTTTC ATATCCTAAG TGCATCACGA GCAATAAGAT GCCGACAGAG
    TTCATCATGT CTGCTACACC TACCATGGGA AACACAGATT TCTGGAAGAC ACTTCGATAC
    TTAAATTTGC TGTACCCTTC CCACGTGTTA CGGAACATCC TCCTCATATC CGACGGGCAC
    CTCCAGAACG AGAGCCTGAC CTTGCAGCTC GTAAGAAGAA GCGTGCCGCA CACCAGGCTG
    TTCTCCTGTG GGGTCAGTTC TACAGCAAAT CGTCATGTCT TACGGAGTCT GTCCCACTAT
    GGCGCTGGAG TATTTGAATA TTTTAACTCA AAATCCAAAC ATAGCTGGAA AAAACAGATA
    GAAGCCCAGA TGGATAGGCT GAGTTCTCCC AGTTGCCACT CGGTCTCCGT CAAATGGCAG
    CAGCTGAGCA CGGACACGCC CGCACCCCTG CAGGCCCCCA CCCAGGTGCA GTCCCTGTTC
    CACAGCGAGC GACTCCTTGT CTACGGGCTC ACTCCTCACT GTACACAGGC AACTCTACAT
    GCGCTGATTC AAGAGAGAGA GTATTCTACA GTGGTGTCAA CTACTGAACT TCAGAAGACA
    ACTGGAACGA TGATTCACAA GCTGGCAGCT CGAGCTCTGA TAAGAGATTA TGAAGATGGC
    ATTCTCCACG AAAACGAAGT GAATCACGAG ATGAAGAAGC AAACTTTAAA ATCTCTGATT
    ATTAAACTCA GTAAAGAAAA CTCTCTCATA ACACAGTTTA CAAGCTTTGT GGCTGTGGAG
    AAAAGGTATG GTAATGAGTC ACCTTCTCCT GATATTCCAA ATACTTTGGA ACTTATTGCC
    AAAGAAGACA TAGATTTTCT GCCATACATG AGCTGGGAGG GGGAACAACC AGATGCCAGC
    AGGATGCAGA CACTTTCAGC ATCGTCTGAA TGGAACCAAG AACCCAAACG TAAATGTAAA
    CGTAAACAGA CTAAAAAGAA GCGTTCATCA CTTAAGATGA TACTTTCTGA AGATTTTAAA
    GCCTTTAGCT CAGATGCCCC AGAAGAATCT GAAGCGGTCA GTTTTGTCTT CAGGGATGCA
    GAGGAGCCCT GGGGTTTGGG TCTGATGGAG TCATCTAAAC AAACAGAAGT TAAACCACAA
    TTCACAGAAG CTATCTCACA GAAAACGGCC TTTTCTGACA TTCCTACTGC CCCTAGTTTC
    TTAACTACAC CCCTCCATGT TGGCTCTGGT TTCTACCTTC AGGCCAGTTC AGCACTGTTT
    GGTTCTCCAG CTGGTCCCAA ACACTTCGGT TCCCCTAAGA AGTACCTGGA AATGGGCAGT
    GGTGCCGACG TTTCCCTGGA CATGGCGTCT TTTCCCAGGC TGCATCTCCA GGGGTCACCT
    TTGCAACTTG CTGCTGCTCC CAGGGTCTCG TTTAGCTCCG GGTCTCAGCT GCCCAAAACC
    AATCAAGGGG GTGCCACAGG CTTCAGTGCC CTCACTACCC TTCAGCCAGA CCCTCTCTCA
    CGGCCGAATC TTTTTTCCTG TTCTGCAAGT CTTCTCCCTT GTCCACTTGT AAAGTCTGTC
    TTTTCTTCCC CTGCCTCTCC TCCCTCCCTA CCCAGGGGCG TTGTTCCCCC TCCTCCCCCT
    CTACCTGGGT GGTGTGGTGT TCTTCCTTCT CCCCCTCTTC CCCCTCTAAC TGGGGGACTT
    GTTCCCCCTC CTCCCCCTCT ATCTCAGAGG GTTGGCATTC CTCCTCCCCC TCCTCCCCCT
    CTACCTGGGT GGTGTGGTGT TCCTCCTTCT CCCCCACTTC CCCCTCTAAC TGGGGGACTT
    GTTCCCCCTC CTCCCCCTCT ACCTGGGTGG GGTGGTGTTC CTCCTCCTCC CCATCTTCCC
    CCTCTATCTC AGGGGGTGGG CATTCCTCCT CCCCCTCCTC CCCCTCTTTC CTCTCCTCTT
    CCCTCTCACC ATCTCCCATC TCCTAGGAGT GCCAGTTATA AGCTGCCAGT GGCTCCATCT
    GGCCCTGCTG AGGATGCCCG GCTGGACATT CCTCTGTTAG CTGTGTATGA TAAGCCTTTG
    TCATTAGACT CCCTCCAATA TGACTGTTCA TTTTTTGAAT GTTCTTCTGA AACGGAAAGT
    GATAAAACAG GAGAACTTCT GGCAAGTAGG ATTTTTAAAA AGCTGGCAGA ATATGACCTG
    AGAAGTGGCT TTACTGCAAG TACTCAGGCA CAAAAAGATG AGGATGGCCT AGTTGGCAAA
    GCAAGCTGGA GGAATTATGT GCCTTCGACT GAACTCTTCA GGCTTCAGAC CAAGGATGGA
    TGCTGGAAAC TTACACAAGA ACTGGGATTT ATATTAAAAC TTAATACAAA TATTTTAAAC
    AGCTTTCTTG AGCAAAAAGG CATTCGATCT TTAGGTGTAA GAGGAAGAGA GCGCCTTCTA
    GATCTGATCG CCACGTTTCT AGTGCTGCAG TTCCTTCGCA CCAAGTTGGA ACAGGAGGGA
    ATAATCGTCA AATCGCTGAT GAGACTGGAT GATGCTTCCA TTCCCAGGAA TATTCCCTGG
    GATTGCGAGA AAATAAAGAA AGCCAGTGAG TGGCTCAGAA GAACTGAAGG GCAGTACCCA
    TCCATCTGCC AGCGGCTGGA GCTGGGTAAA GACTGGGACT CCGCCACCAA GCAGCTGCTG
    GGTATCCTGC CCAGAGATGC CGCCTCCCCT CCTCACAGGC TCCTTAAGCA CAGCCAAGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002691914.2
    CDS133..5595
    Translation

    Target ORF information:

    RefSeq Version XM_002691868.5
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(ADP-ribose) polymerase family member 4 (PARP4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691868.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGACTGTGG GAATCTTCGC AAACTGTACC TTCTGTTTGA AAGCCAAGCA CTTAACTCGT 
    CAACAGAAGA GAAAACTACA AACTGATATT AAGGAAAATG GTGGAAATTT TTCCTTTTTA
    TTAAATGCTC AGTGCACACA TGTAATCTTA GACAATGCTG ATGTTCTGAG CCAGTATCAG
    CTGAAGTCTA TTCAGAAGAA CCATATTCTT ATTGCAAACC CAGATTTTGT ATGGGAATCT
    ATCAAAGAAA GAAGACTGCT GGATACAAAG ATTTATGGTC CCAGTGAGTC ACTGGACATC
    ACATCAGCTT CCAGTGAAGA ATGGAGCAGC CCTAAAATGG AAATGAATGA TTCTTCATTC
    CTGGACAGTT CCACAGAGAA GGAGAACACT GTTGAACTCA CTAAGTTTTA TGCAGAGGGT
    GTTGAAATTC CTCATTTTCC TCAAGATTTT GAAGTTGCAA AATATAACAT CTTGGAAAAA
    GTTGTGACAG AGGGAGGTAA GGAGACCGCC GTGGTGGAGC TGCAGTGCCT CCAGGGCCCT
    GGGGACCGTG GCTTTCTGAT ATGCACACAC TTCCTCCTGG CCACTGGCAC CCAGACCAGA
    AGGCAGTCCT CTGTGAAGAA AACCTCTGCA GACGCAAGTG AATACTATGA GAATTATATT
    GAGGAACTGA AGAAACAAGG CTTTCTACTG AGAGAACATT TTACACCTGA AGCAACCCAG
    TTAGCATCTG AAAAACTGCA AGCATTGCTT TTGGAGGAGG TAATAAACTC GAATGCCCTG
    AGCCCCGAGG TGAGCGGCTT GGTGGAGATG CTCTGGGCAG AAGCCCTCGG CCGCCTGGAG
    GACACACTGC TGAGACCTGT GGGCATGATG AGCCTGAATG ACGTGAGCAA GGCAGAGGGA
    ATTCTCCTCC TGGTAAAGGA AGCAATGAGA AATGGAGAAA CACAGGAGCA ACTGCAAACG
    ATGCTGACCG AGTTCTACAG ATTAATCCCT CACAGAGCTG CTGTGGCCGA GAAGGTCAGC
    CTGAGCCTGC TGGCTAAGAA GGAGGACCTG TGCCAGCTAA TAAGAGACAT GGTTAATGTC
    TGTGAAACTA ATTTGGCCAA ACCCAACCCC CCATCTCTTG CCAAATACCG AGCTTTGAGA
    TGCAAAATTG AGCATGTTGA GCCGAACACT GAAGAATTTT TGAGAGTAAG AGAAGAAGTG
    CTGCAGAATA ATCACAGTAA GGGTCCAGTG GACATCTTGC AGATATTTAG AGTTGGCAGA
    GTGAGTGAAA CCACAGAGTT CCAGGGCCAC CTTGGCAATG TCAGGCACTT ACTGCATGGG
    TCTCCTGTGC AGAGCTTCAT GGGAATCCTG TCCCGTGGGT TGCTTTTACC CAAAGTAGTT
    GAAGATCGTG GCATGAAGAG AACAGACATT GGAAATCTTG GGAGTGGGAT ATATTTCAGC
    GATTCAATCA GTACAAGCAT CAAGTACTCG CACCCTGGAC AGACAGATGG CGCCCGACTC
    CTGGTCGTCT GTGATGTCGC ACTCGGCAGG TGCATGGACC TGCACAAGCG AGACTTCTCC
    CTGACGGAGG CACCACCCGA GTACGACAGC GTCCACGGGG TGCGGGGAAC GGATATGGTC
    AGCTCGGACT TTGAGGATGA TGAATTTGTT GTATACAAAA CCAATCAGGT TAAAATGAAA
    TATCTTGTTA AATTTTCTGT ACCTGGAGAT GAAATAAAGG ACTTTCATCC TTGTGACAAT
    ACGGAACTAG AGGAAGACAG ACCTGAGTTT TCAAATTTTT CAGCAGTTGA AGATCACCAG
    CTTCCAGGCA GCACGCCTTT CCCCGACATC AAGGCCGGCC TCCAGGATGC CTCTGGGAAT
    TCAGTTCCTC TGGAGGACGT TCACATCAAG GGGAAAATAA TAGACTTTGT AGCCCAGGTC
    ATTGTTTTTC AAACATACAC AAACCAAAGT CATGTGCCCA TTGAGGCAAA ATATGTTTTT
    CCTTTGGACG ACAAGGCAGC TGTATGTGGT TTCGAGGCTT TCATCAATGG GAAGCACGTG
    GTGGGAGAGA TTAAAGAGAA GGAAGTGGCC CAGCGAGAGT ACCGAGTGGC GATCAGTCAA
    GGTCATGGCG CCTACCTGAT GGATCAGGAC ACACCCGACA TCTTCACCGT CAGCGTCGGG
    AACTTACCCC CAAAGGCCAA AGTCCTGGTC AAAATCACCT ACATCACGGA GCTGAGCATC
    CAGGGTGCCT GCGCGGTCTT CTTCCTGCCG GCCACGGTGG CGCCCTGGCA GCAGGACAGG
    GCTCTGAGGG AGAACCTTCA GGATACAGTG GAGAAGATTT GTATAAAGGA AATAGGAGCA
    AAGCAAAGCT TTTCTCTGAG CATGTCTATC GAGATGCCGC ACATGATTGA ATTCATCTCC
    AGTGACACAC ACGACCTGAG AAAAAAGAGA ACAGACTGCA AAGCTGTGAT TAGTACTGTG
    GAAGGCAGCT CCTTAGACAG CAATGGATTT TCTCTCCTCA TCGGTTTGGT TGATGTGTAT
    CTCCCAAGAA TGTGGGTTGA AAAGCATCCA GAAAAAGAAA GCGAGGCCTG CATGCTTGTG
    TTTCAACCAA ATCTTGGTAT TACCGTCCCT GACGAAGCTG AGAGGAGTGA AGTGATTATT
    TGTCTTGATT GCTCCAATTC CATGGAGGGC GAGACATTCC TGCAAGCCAA ACAGATTGCC
    TTGCATGCAC TGTCCTGTGT GGGAAAGGAG CAGAAGGTGA ACGTCATCAA GTTCGGCACA
    AGTTACAAGG AATTATTTTC ATATCCTAAG TGCATCACGA GCAATAAGAT GCCGACAGAG
    TTCATCATGT CTGCTACACC TACCATGGGA AACACAGATT TCTGGAAGAC ACTTCGATAC
    TTAAATTTGC TGTACCCTTC CCACGTGTTA CGGAACATCC TCCTCATATC CGACGGGCAC
    CTCCAGAACG AGAGCCTGAC CTTGCAGCTC GTAAGAAGAA GCGTGCCGCA CACCAGGCTG
    TTCTCCTGTG GGGTCAGTTC TACAGCAAAT CGTCATGTCT TACGGAGTCT GTCCCACTAT
    GGCGCTGGAG TATTTGAATA TTTTAACTCA AAATCCAAAC ATAGCTGGAA AAAACAGATA
    GAAGCCCAGA TGGATAGGCT GAGTTCTCCC AGTTGCCACT CGGTCTCCGT CAAATGGCAG
    CAGCTGAGCA CGGACACGCC CGCACCCCTG CAGGCCCCCA CCCAGGTGCA GTCCCTGTTC
    CACAGCGAGC GACTCCTTGT CTACGGGCTC ACTCCTCACT GTACACAGGC AACTCTACAT
    GCGCTGATTC AAGAGAGAGA GTATTCTACA GTGGTGTCAA CTACTGAACT TCAGAAGACA
    ACTGGAACGA TGATTCACAA GCTGGCAGCT CGAGCTCTGA TAAGAGATTA TGAAGATGGC
    ATTCTCCACG AAAACGAAGT GAATCACGAG ATGAAGAAGC AAACTTTAAA ATCTCTGATT
    ATTAAACTCA GTAAAGAAAA CTCTCTCATA ACACAGTTTA CAAGCTTTGT GGCTGTGGAG
    AAAAGGTATG GTAATGAGTC ACCTTCTCCT GATATTCCAA ATACTTTGGA ACTTATTGCC
    AAAGAAGACA TAGATTTTCT GCCATACATG AGCTGGGAGG GGGAACAACC AGATGCCAGC
    AGGATGCAGA CACTTTCAGC ATCGTCTGAA TGGAACCAAG AACCCAAACG TAAATGTAAA
    CGTAAACAGA CTAAAAAGAA GCGTTCATCA CTTAAGATGA TACTTTCTGA AGATTTTAAA
    GCCTTTAGCT CAGATGCCCC AGAAGAATCT GAAGCGGTCA GTTTTGTCTT CAGGGATGCA
    GAGGAGCCCT GGGGTTTGGG TCTGATGGAG TCATCTAAAC AAACAGAAGT TAAACCACAA
    TTCACAGAAG CTATCTCACA GAAAACGGCC TTTTCTGACA TTCCTACTGC CCCTAGTTTC
    TTAACTACAC CCCTCCATGT TGGCTCTGGT TTCTACCTTC AGGCCAGTTC AGCACTGTTT
    GGTTCTCCAG CTGGTCCCAA ACACTTCGGT TCCCCTAAGA AGTACCTGGA AATGGGCAGT
    GGTGCCGACG TTTCCCTGGA CATGGCGTCT TTTCCCAGGC TGCATCTCCA GGGGTCACCT
    TTGCAACTTG CTGCTGCTCC CAGGGTCTCG TTTAGCTCCG GGTCTCAGCT GCCCAAAACC
    AATCAAGGGG GTGCCACAGG CTTCAGTGCC CTCACTACCC TTCAGCCAGA CCCTCTCTCA
    CGGCCGAATC TTTTTTCCTG TTCTGCAAGT CTTCTCCCTT GTCCACTTGT AAAGTCTGTC
    TTTTCTTCCC CTGCCTCTCC TCCCTCCCTA CCCAGGGGCG TTGTTCCCCC TCCTCCCCCT
    CTACCTGGGT GGTGTGGTGT TCTTCCTTCT CCCCCTCTTC CCCCTCTAAC TGGGGGACTT
    GTTCCCCCTC CTCCCCCTCT ATCTCAGAGG GTTGGCATTC CTCCTCCCCC TCCTCCCCCT
    CTACCTGGGT GGTGTGGTGT TCCTCCTTCT CCCCCACTTC CCCCTCTAAC TGGGGGACTT
    GTTCCCCCTC CTCCCCCTCT ACCTGGGTGG GGTGGTGTTC CTCCTCCTCC CCATCTTCCC
    CCTCTATCTC AGGGGGTGGG CATTCCTCCT CCCCCTCCTC CCCCTCTTTC CTCTCCTCTT
    CCCTCTCACC ATCTCCCATC TCCTAGGAGT GCCAGTTATA AGCTGCCAGT GGCTCCATCT
    GGCCCTGCTG AGGATGCCCG GCTGGACATT CCTCTGTTAG CTGTGTATGA TAAGCCTTTG
    TCATTAGACT CCCTCCAATA TGACTGTTCA TTTTTTGAAT GTTCTTCTGA AACGGAAAGT
    GATAAAACAG GAGAACTTCT GGCAAGTAGG ATTTTTAAAA AGCTGGCAGA ATATGACCTG
    AGAAGTGGCT TTACTGCAAG TACTCAGGCA CAAAAAGATG AGGATGGCCT AGTTGGCAAA
    GCAAGCTGGA GGAATTATGT GCCTTCGACT GAACTCTTCA GGCTTCAGAC CAAGGATGGA
    TGCTGGAAAC TTACACAAGA ACTGGGATTT ATATTAAAAC TTAATACAAA TATTTTAAAC
    AGCTTTCTTG AGCAAAAAGG CATTCGATCT TTAGGTGTAA GAGGAAGAGA GCGCCTTCTA
    GATCTGATCG CCACGTTTCT AGTGCTGCAG TTCCTTCGCA CCAAGTTGGA ACAGGAGGGA
    ATAATCGTCA AATCGCTGAT GAGACTGGAT GATGCTTCCA TTCCCAGGAA TATTCCCTGG
    GATTGCGAGA AAATAAAGAA AGCCAGTGAG TGGCTCAGAA GAACTGAAGG GCAGTACCCA
    TCCATCTGCC AGCGGCTGGA GCTGGGTAAA GACTGGGACT CCGCCACCAA GCAGCTGCTG
    GGTATCCTGC CCAGAGATGC CGCCTCCCCT CCTCACAGGC TCCTTAAGCA CAGCCAAGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92120
    Clone ID Related Accession (Same CDS sequence) XM_015465709.1 , XM_015473840.1
    Accession Version XM_015465709.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product poly [ADP-ribose] polymerase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645955.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTCTGGG CAGAAGCCCT CGGCCGCCTG GAGGACACAC TGCTGAGACC TGTGGGCATG 
    ATGAGCCTGA ATGACGTGAG CAAGGCAGAG GGAATTCTCC TCCTGGTAAA GGAAGCAATG
    AGAAATGGAG AAACACAGGA GCAACTGCAA ACGATGCTGA CCGAGTTCTA CAGATTAATC
    CCTCACAGAG CTGCTGTGGC CGAGAAGGTC AGCCTGAGCC TGCTGGCTAA GAAGGAGGAC
    CTGTGCCAGC TAATAAGAGA CATGGTTAAT GTCTGTGAAA CTAATTTGGC CAAACCCAAC
    CCCCCATCTC TTGCCAAATA CCGAGCTTTG AGATGCAAAA TTGAGCATGT TGAGCCGAAC
    ACTGAAGAAT TTTTGAGAGT AAGAGAAGAA GTGCTGCAGA ATAATCACAG TAAGGGTCCA
    GTGGACATCT TGCAGATATT TAGAGTTGGC AGAGTGAGTG AAACCACAGA GTTCCAGGGC
    CACCTTGGCA ATGTCAGGCA CTTACTGCAT GGGTCTCCTG TGCAGAGCTT CATGGGAATC
    CTGTCCCGTG GGTTGCTTTT ACCCAAAGTA GTTGAAGATC GTGGCATGAA GAGAACAGAC
    ATTGGAAATC TTGGGAGTGG GATATATTTC AGCGATTCAA TCAGTACAAG CATCAAGTAC
    TCGCACCCTG GACAGACAGA TGGCGCCCGA CTCCTGGTCG TCTGTGATGT CGCACTCGGC
    AGGTGCATGG ACCTGCACAA GCGAGACTTC TCCCTGACGG AGGCACCACC CGAGTACGAC
    AGCGTCCACG GGGTGCGGGG AACGGATATG GTCAGCTCGG ACTTTGAGGA TGATGAATTT
    GTTGTATACA AAACCAATCA GGTTAAAATG AAATATCTTG TTAAATTTTC TGTACCTGGA
    GATGAAATAA AGGACTTTCA TCCTTGTGAC AATACGGAAC TAGAGGAAGA CAGACCTGAG
    TTTTCAAATT TTTCAGCAGT TGAAGATCAC CAGCTTCCAG GCAGCACGCC TTTCCCCGAC
    ATCAAGGCCG GCCTCCAGGA TGCCTCTGGG AATTCAGTTC CTCTGGAGGA CGTTCACATC
    AAGGGGAAAA TAATAGACTT TGTAGCCCAG GTCATTGTTT TTCAAACATA CACAAACCAA
    AGTCATGTGC CCATTGAGGC AAAATATGTT TTTCCTTTGG ACGACAAGGC AGCTGTATGT
    GGTTTCGAGG CTTTCATCAA TGGGAAGCAC GTGGTGGGAG AGATTAAAGA GAAGGAAGTG
    GCCCAGCGAG AGTACCGAGT GGCGATCAGT CAAGGTCATG GCGCCTACCT GATGGATCAG
    GACACACCCG ACATCTTCAC CGTCAGCGTC GGGAACTTAC CCCCAAAGGC CAAAGTCCTG
    GTCAAAATCA CCTACATCAC GGAGCTGAGC ATCCAGGGTG CCTGCGCGGT CTTCTTCCTG
    CCGGCCACGG TGGCGCCCTG GCAGCAGGAC AGGGCTCTGA GGGAGAACCT TCAGGATACA
    GTGGAGAAGA TTTGTATAAA GGAAATAGGA GCAAAGCAAA GCTTTTCTCT GAGCATGTCT
    ATCGAGATGC CGCACATGAT TGAATTCATC TCCAGTGACA CACACGACCT GAGAAAAAAG
    AGAACAGACT GCAAAGCTGT GATTAGTACT GTGGAAGGCA GCTCCTTAGA CAGCAATGGA
    TTTTCTCTCC TCATCGGTTT GGTTGATGTG TATCTCCCAA GAATGTGGGT TGAAAAGCAT
    CCAGAAAAAG AAAGCGAGGC CTGCATGCTT GTGTTTCAAC CAAATCTTGG TATTACCGTC
    CCTGACGAAG CTGAGAGGAG TGAAGTGATT ATTTGTCTTG ATTGCTCCAA TTCCATGGAG
    GGCGAGACAT TCCTGCAAGC CAAACAGATT GCCTTGCATG CACTGTCCTG TGTGGGAAAG
    GAGCAGAAGG TGAACGTCAT CAAGTTCGGC ACAAGTTACA AGGAATTATT TTCATATCCT
    AAGTGCATCA CGAGCAATAA GATGCCGACA GAGTTCATCA TGTCTGCTAC ACCTACCATG
    GGAAACACAG ATTTCTGGAA GACACTTCGA TACTTAAATT TGCTGTACCC TTCCCACGTG
    TTACGGAACA TCCTCCTCAT ATCCGACGGG CACCTCCAGA ACGAGAGCCT GACCTTGCAG
    CTCGTAAGAA GAAGCGTGCC GCACACCAGG CTGTTCTCCT GTGGGGTCAG TTCTACAGCA
    AATCGTCATG TCTTACGGAG TCTGTCCCAC TATGGCGCTG GAGTATTTGA ATATTTTAAC
    TCAAAATCCA AACATAGCTG GAAAAAACAG ATAGAAGCCC AGATGGATAG GCTGAGTTCT
    CCCAGTTGCC ACTCGGTCTC CGTCAAATGG CAGCAGCTGA GCACGGACAC GCCCGCACCC
    CTGCAGGCCC CCACCCAGGT GCAGTCCCTG TTCCACAGCG AGCGACTCCT TGTCTACGGG
    CTCACTCCTC ACTGTACACA GGCAACTCTA CATGCGCTGA TTCAAGAGAG AGAGTATTCT
    ACAGTGGTGT CAACTACTGA ACTTCAGAAG ACAACTGGAA CGATGATTCA CAAGCTGGCA
    GCTCGAGCTC TGATAAGAGA TTATGAAGAT GGCATTCTCC ACGAAAACGA AGTGAATCAC
    GAGATGAAGA AGCAAACTTT AAAATCTCTG ATTATTAAAC TCAGTAAAGA AAACTCTCTC
    ATAACACAGT TTACAAGCTT TGTGGCTGTG GAGAAAAGGT ATGGTAATGA GTCACCTTCT
    CCTGATATTC CAAATACTTT GGAACTTATT GCCAAAGAAG ACATAGATTT TCTGCCATAC
    ATGAGCTGGG AGGGGGAACA ACCAGATGCC AGCAGGATGC AGACACTTTC AGCATCGTCT
    GAATGGAACC AAGAACCCAA ACGTAAATGT AAACGTAAAC AGACTAAAAA GAAGCGTTCA
    TCACTTAAGA TGATACTTTC TGAAGATTTT AAAGCCTTTA GCTCAGATGC CCCAGAAGAA
    TCTGAAGCGG TCAGTTTTGT CTTCAGGGAT GCAGAGGAGC CCTGGGGTTT GGGTCTGATG
    GAGTCATCTA AACAAACAGA AGTTAAACCA CAATTCACAG AAGCTATCTC ACAGAAAACG
    GCCTTTTCTG ACATTCCTAC TGCCCCTAGT TTCTTAACTA CACCCCTCCA TGTTGGCTCT
    GGTTTCTACC TTCAGGCCAG TTCAGCACTG TTTGGTTCTC CAGCTGGTCC CAAACACTTC
    GGTTCCCCTA AGAAGTACCT GGAAATGGGC AGTGGTGCCG ACGTTTCCCT GGACATGGCG
    TCTTTTCCCA GGCTGCATCT CCAGGGGTCA CCTTTGCAAC TTGCTGCTGC TCCCAGGGTC
    TCGTTTAGCT CCGGGTCTCA GCTGCCCAAA ACCAATCAAG GGGGTGCCAC AGGCTTCAGT
    GCCCTCACTA CCCTTCAGCC AGACCCTCTC TCACGGCCGA ATCTTTTTTC CTGTTCTGCA
    AGTCTTCTCC CTTGTCCACT TGTAAAGTCT GTCTTTTCTT CCCCTGCCTC TCCTCCCTCC
    CTACCCAGGG GCGTTGTTCC CCCTCCTCCC CCTCTACCTG GGTGGTGTGG TGTTCTTCCT
    TCTCCCCCTC TTCCCCCTCT AACTGGGGGA CTTGTTCCCC CTCCTCCCCC TCTATCTCAG
    AGGGTTGGCA TTCCTCCTCC CCCTCCTCCC CCTCTACCTG GGTGGTGTGG TGTTCCTCCT
    TCTCCCCCAC TTCCCCCTCT AACTGGGGGA CTTGTTCCCC CTCCTCCCCC TCTACCTGGG
    TGGGGTGGTG TTCCTCCTCC TCCCCATCTT CCCCCTCTAT CTCAGGGGGT GGGCATTCCT
    CCTCCCCCTC CTCCCCCTCT TTCCTCTCCT CTTCCCTCTC ACCATCTCCC ATCTCCTAGG
    AGTGCCAGTT ATAAGCTGCC AGTGGCTCCA TCTGGCCCTG CTGAGGATGC CCGGCTGGAC
    ATTCCTCTGT TAGCTGTGTA TGATAAGCCT TTGTCATTAG ACTCCCTCCA ATATGACTGT
    TCATTTTTTG AATGTTCTTC TGAAACGGAA AGTGATAAAA CAGGAGAACT TCTGGCAAGT
    AGGATTTTTA AAAAGCTGGC AGAATATGAC CTGAGAAGTG GCTTTACTGC AAGTACTCAG
    GCACAAAAAG ATGAGGATGG CCTAGTTGGC AAAGCAAGCT GGAGGAATTA TGTGCCTTCG
    ACTGAACTCT TCAGGCTTCA GACCAAGGAT GGATGCTGGA AACTTACACA AGAACTGGGA
    TTTATATTAA AACTTAATAC AAATATTTTA AACAGCTTTC TTGAGCAAAA AGGCATTCGA
    TCTTTAGGTG TAAGAGGAAG AGAGCGCCTT CTAGATCTGA TCGCCACGTT TCTAGTGCTG
    CAGTTCCTTC GCACCAAGTT GGAACAGGAG GGAATAATCG TCAAATCGCT GATGAGACTG
    GATGATGCTT CCATTCCCAG GAATATTCCC TGGGATTGCG AGAAAATAAA GAAAGCCAGT
    GAGTGGCTCA GAAGAACTGA AGGGCAGTAC CCATCCATCT GCCAGCGGCT GGAGCTGGGT
    AAAGACTGGG ACTCCGCCAC CAAGCAGCTG CTGGGTATCC TGCCCAGAGA TGCCGCCTCC
    CCTCCTCACA GGCTCCTTAA GCACAGCCAA GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321195.1
    CDS393..5048
    Translation

    Target ORF information:

    RefSeq Version XM_015465709.1
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(ADP-ribose) polymerase family member 4 (PARP4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465709.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTCTGGG CAGAAGCCCT CGGCCGCCTG GAGGACACAC TGCTGAGACC TGTGGGCATG 
    ATGAGCCTGA ATGACGTGAG CAAGGCAGAG GGAATTCTCC TCCTGGTAAA GGAAGCAATG
    AGAAATGGAG AAACACAGGA GCAACTGCAA ACGATGCTGA CCGAGTTCTA CAGATTAATC
    CCTCACAGAG CTGCTGTGGC CGAGAAGGTC AGCCTGAGCC TGCTGGCTAA GAAGGAGGAC
    CTGTGCCAGC TAATAAGAGA CATGGTTAAT GTCTGTGAAA CTAATTTGGC CAAACCCAAC
    CCCCCATCTC TTGCCAAATA CCGAGCTTTG AGATGCAAAA TTGAGCATGT TGAGCCGAAC
    ACTGAAGAAT TTTTGAGAGT AAGAGAAGAA GTGCTGCAGA ATAATCACAG TAAGGGTCCA
    GTGGACATCT TGCAGATATT TAGAGTTGGC AGAGTGAGTG AAACCACAGA GTTCCAGGGC
    CACCTTGGCA ATGTCAGGCA CTTACTGCAT GGGTCTCCTG TGCAGAGCTT CATGGGAATC
    CTGTCCCGTG GGTTGCTTTT ACCCAAAGTA GTTGAAGATC GTGGCATGAA GAGAACAGAC
    ATTGGAAATC TTGGGAGTGG GATATATTTC AGCGATTCAA TCAGTACAAG CATCAAGTAC
    TCGCACCCTG GACAGACAGA TGGCGCCCGA CTCCTGGTCG TCTGTGATGT CGCACTCGGC
    AGGTGCATGG ACCTGCACAA GCGAGACTTC TCCCTGACGG AGGCACCACC CGAGTACGAC
    AGCGTCCACG GGGTGCGGGG AACGGATATG GTCAGCTCGG ACTTTGAGGA TGATGAATTT
    GTTGTATACA AAACCAATCA GGTTAAAATG AAATATCTTG TTAAATTTTC TGTACCTGGA
    GATGAAATAA AGGACTTTCA TCCTTGTGAC AATACGGAAC TAGAGGAAGA CAGACCTGAG
    TTTTCAAATT TTTCAGCAGT TGAAGATCAC CAGCTTCCAG GCAGCACGCC TTTCCCCGAC
    ATCAAGGCCG GCCTCCAGGA TGCCTCTGGG AATTCAGTTC CTCTGGAGGA CGTTCACATC
    AAGGGGAAAA TAATAGACTT TGTAGCCCAG GTCATTGTTT TTCAAACATA CACAAACCAA
    AGTCATGTGC CCATTGAGGC AAAATATGTT TTTCCTTTGG ACGACAAGGC AGCTGTATGT
    GGTTTCGAGG CTTTCATCAA TGGGAAGCAC GTGGTGGGAG AGATTAAAGA GAAGGAAGTG
    GCCCAGCGAG AGTACCGAGT GGCGATCAGT CAAGGTCATG GCGCCTACCT GATGGATCAG
    GACACACCCG ACATCTTCAC CGTCAGCGTC GGGAACTTAC CCCCAAAGGC CAAAGTCCTG
    GTCAAAATCA CCTACATCAC GGAGCTGAGC ATCCAGGGTG CCTGCGCGGT CTTCTTCCTG
    CCGGCCACGG TGGCGCCCTG GCAGCAGGAC AGGGCTCTGA GGGAGAACCT TCAGGATACA
    GTGGAGAAGA TTTGTATAAA GGAAATAGGA GCAAAGCAAA GCTTTTCTCT GAGCATGTCT
    ATCGAGATGC CGCACATGAT TGAATTCATC TCCAGTGACA CACACGACCT GAGAAAAAAG
    AGAACAGACT GCAAAGCTGT GATTAGTACT GTGGAAGGCA GCTCCTTAGA CAGCAATGGA
    TTTTCTCTCC TCATCGGTTT GGTTGATGTG TATCTCCCAA GAATGTGGGT TGAAAAGCAT
    CCAGAAAAAG AAAGCGAGGC CTGCATGCTT GTGTTTCAAC CAAATCTTGG TATTACCGTC
    CCTGACGAAG CTGAGAGGAG TGAAGTGATT ATTTGTCTTG ATTGCTCCAA TTCCATGGAG
    GGCGAGACAT TCCTGCAAGC CAAACAGATT GCCTTGCATG CACTGTCCTG TGTGGGAAAG
    GAGCAGAAGG TGAACGTCAT CAAGTTCGGC ACAAGTTACA AGGAATTATT TTCATATCCT
    AAGTGCATCA CGAGCAATAA GATGCCGACA GAGTTCATCA TGTCTGCTAC ACCTACCATG
    GGAAACACAG ATTTCTGGAA GACACTTCGA TACTTAAATT TGCTGTACCC TTCCCACGTG
    TTACGGAACA TCCTCCTCAT ATCCGACGGG CACCTCCAGA ACGAGAGCCT GACCTTGCAG
    CTCGTAAGAA GAAGCGTGCC GCACACCAGG CTGTTCTCCT GTGGGGTCAG TTCTACAGCA
    AATCGTCATG TCTTACGGAG TCTGTCCCAC TATGGCGCTG GAGTATTTGA ATATTTTAAC
    TCAAAATCCA AACATAGCTG GAAAAAACAG ATAGAAGCCC AGATGGATAG GCTGAGTTCT
    CCCAGTTGCC ACTCGGTCTC CGTCAAATGG CAGCAGCTGA GCACGGACAC GCCCGCACCC
    CTGCAGGCCC CCACCCAGGT GCAGTCCCTG TTCCACAGCG AGCGACTCCT TGTCTACGGG
    CTCACTCCTC ACTGTACACA GGCAACTCTA CATGCGCTGA TTCAAGAGAG AGAGTATTCT
    ACAGTGGTGT CAACTACTGA ACTTCAGAAG ACAACTGGAA CGATGATTCA CAAGCTGGCA
    GCTCGAGCTC TGATAAGAGA TTATGAAGAT GGCATTCTCC ACGAAAACGA AGTGAATCAC
    GAGATGAAGA AGCAAACTTT AAAATCTCTG ATTATTAAAC TCAGTAAAGA AAACTCTCTC
    ATAACACAGT TTACAAGCTT TGTGGCTGTG GAGAAAAGGT ATGGTAATGA GTCACCTTCT
    CCTGATATTC CAAATACTTT GGAACTTATT GCCAAAGAAG ACATAGATTT TCTGCCATAC
    ATGAGCTGGG AGGGGGAACA ACCAGATGCC AGCAGGATGC AGACACTTTC AGCATCGTCT
    GAATGGAACC AAGAACCCAA ACGTAAATGT AAACGTAAAC AGACTAAAAA GAAGCGTTCA
    TCACTTAAGA TGATACTTTC TGAAGATTTT AAAGCCTTTA GCTCAGATGC CCCAGAAGAA
    TCTGAAGCGG TCAGTTTTGT CTTCAGGGAT GCAGAGGAGC CCTGGGGTTT GGGTCTGATG
    GAGTCATCTA AACAAACAGA AGTTAAACCA CAATTCACAG AAGCTATCTC ACAGAAAACG
    GCCTTTTCTG ACATTCCTAC TGCCCCTAGT TTCTTAACTA CACCCCTCCA TGTTGGCTCT
    GGTTTCTACC TTCAGGCCAG TTCAGCACTG TTTGGTTCTC CAGCTGGTCC CAAACACTTC
    GGTTCCCCTA AGAAGTACCT GGAAATGGGC AGTGGTGCCG ACGTTTCCCT GGACATGGCG
    TCTTTTCCCA GGCTGCATCT CCAGGGGTCA CCTTTGCAAC TTGCTGCTGC TCCCAGGGTC
    TCGTTTAGCT CCGGGTCTCA GCTGCCCAAA ACCAATCAAG GGGGTGCCAC AGGCTTCAGT
    GCCCTCACTA CCCTTCAGCC AGACCCTCTC TCACGGCCGA ATCTTTTTTC CTGTTCTGCA
    AGTCTTCTCC CTTGTCCACT TGTAAAGTCT GTCTTTTCTT CCCCTGCCTC TCCTCCCTCC
    CTACCCAGGG GCGTTGTTCC CCCTCCTCCC CCTCTACCTG GGTGGTGTGG TGTTCTTCCT
    TCTCCCCCTC TTCCCCCTCT AACTGGGGGA CTTGTTCCCC CTCCTCCCCC TCTATCTCAG
    AGGGTTGGCA TTCCTCCTCC CCCTCCTCCC CCTCTACCTG GGTGGTGTGG TGTTCCTCCT
    TCTCCCCCAC TTCCCCCTCT AACTGGGGGA CTTGTTCCCC CTCCTCCCCC TCTACCTGGG
    TGGGGTGGTG TTCCTCCTCC TCCCCATCTT CCCCCTCTAT CTCAGGGGGT GGGCATTCCT
    CCTCCCCCTC CTCCCCCTCT TTCCTCTCCT CTTCCCTCTC ACCATCTCCC ATCTCCTAGG
    AGTGCCAGTT ATAAGCTGCC AGTGGCTCCA TCTGGCCCTG CTGAGGATGC CCGGCTGGAC
    ATTCCTCTGT TAGCTGTGTA TGATAAGCCT TTGTCATTAG ACTCCCTCCA ATATGACTGT
    TCATTTTTTG AATGTTCTTC TGAAACGGAA AGTGATAAAA CAGGAGAACT TCTGGCAAGT
    AGGATTTTTA AAAAGCTGGC AGAATATGAC CTGAGAAGTG GCTTTACTGC AAGTACTCAG
    GCACAAAAAG ATGAGGATGG CCTAGTTGGC AAAGCAAGCT GGAGGAATTA TGTGCCTTCG
    ACTGAACTCT TCAGGCTTCA GACCAAGGAT GGATGCTGGA AACTTACACA AGAACTGGGA
    TTTATATTAA AACTTAATAC AAATATTTTA AACAGCTTTC TTGAGCAAAA AGGCATTCGA
    TCTTTAGGTG TAAGAGGAAG AGAGCGCCTT CTAGATCTGA TCGCCACGTT TCTAGTGCTG
    CAGTTCCTTC GCACCAAGTT GGAACAGGAG GGAATAATCG TCAAATCGCT GATGAGACTG
    GATGATGCTT CCATTCCCAG GAATATTCCC TGGGATTGCG AGAAAATAAA GAAAGCCAGT
    GAGTGGCTCA GAAGAACTGA AGGGCAGTAC CCATCCATCT GCCAGCGGCT GGAGCTGGGT
    AAAGACTGGG ACTCCGCCAC CAAGCAGCTG CTGGGTATCC TGCCCAGAGA TGCCGCCTCC
    CCTCCTCACA GGCTCCTTAA GCACAGCCAA GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92120
    Clone ID Related Accession (Same CDS sequence) XM_015465709.1 , XM_015473840.1
    Accession Version XM_015473840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product poly [ADP-ribose] polymerase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104312.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTCTGGG CAGAAGCCCT CGGCCGCCTG GAGGACACAC TGCTGAGACC TGTGGGCATG 
    ATGAGCCTGA ATGACGTGAG CAAGGCAGAG GGAATTCTCC TCCTGGTAAA GGAAGCAATG
    AGAAATGGAG AAACACAGGA GCAACTGCAA ACGATGCTGA CCGAGTTCTA CAGATTAATC
    CCTCACAGAG CTGCTGTGGC CGAGAAGGTC AGCCTGAGCC TGCTGGCTAA GAAGGAGGAC
    CTGTGCCAGC TAATAAGAGA CATGGTTAAT GTCTGTGAAA CTAATTTGGC CAAACCCAAC
    CCCCCATCTC TTGCCAAATA CCGAGCTTTG AGATGCAAAA TTGAGCATGT TGAGCCGAAC
    ACTGAAGAAT TTTTGAGAGT AAGAGAAGAA GTGCTGCAGA ATAATCACAG TAAGGGTCCA
    GTGGACATCT TGCAGATATT TAGAGTTGGC AGAGTGAGTG AAACCACAGA GTTCCAGGGC
    CACCTTGGCA ATGTCAGGCA CTTACTGCAT GGGTCTCCTG TGCAGAGCTT CATGGGAATC
    CTGTCCCGTG GGTTGCTTTT ACCCAAAGTA GTTGAAGATC GTGGCATGAA GAGAACAGAC
    ATTGGAAATC TTGGGAGTGG GATATATTTC AGCGATTCAA TCAGTACAAG CATCAAGTAC
    TCGCACCCTG GACAGACAGA TGGCGCCCGA CTCCTGGTCG TCTGTGATGT CGCACTCGGC
    AGGTGCATGG ACCTGCACAA GCGAGACTTC TCCCTGACGG AGGCACCACC CGAGTACGAC
    AGCGTCCACG GGGTGCGGGG AACGGATATG GTCAGCTCGG ACTTTGAGGA TGATGAATTT
    GTTGTATACA AAACCAATCA GGTTAAAATG AAATATCTTG TTAAATTTTC TGTACCTGGA
    GATGAAATAA AGGACTTTCA TCCTTGTGAC AATACGGAAC TAGAGGAAGA CAGACCTGAG
    TTTTCAAATT TTTCAGCAGT TGAAGATCAC CAGCTTCCAG GCAGCACGCC TTTCCCCGAC
    ATCAAGGCCG GCCTCCAGGA TGCCTCTGGG AATTCAGTTC CTCTGGAGGA CGTTCACATC
    AAGGGGAAAA TAATAGACTT TGTAGCCCAG GTCATTGTTT TTCAAACATA CACAAACCAA
    AGTCATGTGC CCATTGAGGC AAAATATGTT TTTCCTTTGG ACGACAAGGC AGCTGTATGT
    GGTTTCGAGG CTTTCATCAA TGGGAAGCAC GTGGTGGGAG AGATTAAAGA GAAGGAAGTG
    GCCCAGCGAG AGTACCGAGT GGCGATCAGT CAAGGTCATG GCGCCTACCT GATGGATCAG
    GACACACCCG ACATCTTCAC CGTCAGCGTC GGGAACTTAC CCCCAAAGGC CAAAGTCCTG
    GTCAAAATCA CCTACATCAC GGAGCTGAGC ATCCAGGGTG CCTGCGCGGT CTTCTTCCTG
    CCGGCCACGG TGGCGCCCTG GCAGCAGGAC AGGGCTCTGA GGGAGAACCT TCAGGATACA
    GTGGAGAAGA TTTGTATAAA GGAAATAGGA GCAAAGCAAA GCTTTTCTCT GAGCATGTCT
    ATCGAGATGC CGCACATGAT TGAATTCATC TCCAGTGACA CACACGACCT GAGAAAAAAG
    AGAACAGACT GCAAAGCTGT GATTAGTACT GTGGAAGGCA GCTCCTTAGA CAGCAATGGA
    TTTTCTCTCC TCATCGGTTT GGTTGATGTG TATCTCCCAA GAATGTGGGT TGAAAAGCAT
    CCAGAAAAAG AAAGCGAGGC CTGCATGCTT GTGTTTCAAC CAAATCTTGG TATTACCGTC
    CCTGACGAAG CTGAGAGGAG TGAAGTGATT ATTTGTCTTG ATTGCTCCAA TTCCATGGAG
    GGCGAGACAT TCCTGCAAGC CAAACAGATT GCCTTGCATG CACTGTCCTG TGTGGGAAAG
    GAGCAGAAGG TGAACGTCAT CAAGTTCGGC ACAAGTTACA AGGAATTATT TTCATATCCT
    AAGTGCATCA CGAGCAATAA GATGCCGACA GAGTTCATCA TGTCTGCTAC ACCTACCATG
    GGAAACACAG ATTTCTGGAA GACACTTCGA TACTTAAATT TGCTGTACCC TTCCCACGTG
    TTACGGAACA TCCTCCTCAT ATCCGACGGG CACCTCCAGA ACGAGAGCCT GACCTTGCAG
    CTCGTAAGAA GAAGCGTGCC GCACACCAGG CTGTTCTCCT GTGGGGTCAG TTCTACAGCA
    AATCGTCATG TCTTACGGAG TCTGTCCCAC TATGGCGCTG GAGTATTTGA ATATTTTAAC
    TCAAAATCCA AACATAGCTG GAAAAAACAG ATAGAAGCCC AGATGGATAG GCTGAGTTCT
    CCCAGTTGCC ACTCGGTCTC CGTCAAATGG CAGCAGCTGA GCACGGACAC GCCCGCACCC
    CTGCAGGCCC CCACCCAGGT GCAGTCCCTG TTCCACAGCG AGCGACTCCT TGTCTACGGG
    CTCACTCCTC ACTGTACACA GGCAACTCTA CATGCGCTGA TTCAAGAGAG AGAGTATTCT
    ACAGTGGTGT CAACTACTGA ACTTCAGAAG ACAACTGGAA CGATGATTCA CAAGCTGGCA
    GCTCGAGCTC TGATAAGAGA TTATGAAGAT GGCATTCTCC ACGAAAACGA AGTGAATCAC
    GAGATGAAGA AGCAAACTTT AAAATCTCTG ATTATTAAAC TCAGTAAAGA AAACTCTCTC
    ATAACACAGT TTACAAGCTT TGTGGCTGTG GAGAAAAGGT ATGGTAATGA GTCACCTTCT
    CCTGATATTC CAAATACTTT GGAACTTATT GCCAAAGAAG ACATAGATTT TCTGCCATAC
    ATGAGCTGGG AGGGGGAACA ACCAGATGCC AGCAGGATGC AGACACTTTC AGCATCGTCT
    GAATGGAACC AAGAACCCAA ACGTAAATGT AAACGTAAAC AGACTAAAAA GAAGCGTTCA
    TCACTTAAGA TGATACTTTC TGAAGATTTT AAAGCCTTTA GCTCAGATGC CCCAGAAGAA
    TCTGAAGCGG TCAGTTTTGT CTTCAGGGAT GCAGAGGAGC CCTGGGGTTT GGGTCTGATG
    GAGTCATCTA AACAAACAGA AGTTAAACCA CAATTCACAG AAGCTATCTC ACAGAAAACG
    GCCTTTTCTG ACATTCCTAC TGCCCCTAGT TTCTTAACTA CACCCCTCCA TGTTGGCTCT
    GGTTTCTACC TTCAGGCCAG TTCAGCACTG TTTGGTTCTC CAGCTGGTCC CAAACACTTC
    GGTTCCCCTA AGAAGTACCT GGAAATGGGC AGTGGTGCCG ACGTTTCCCT GGACATGGCG
    TCTTTTCCCA GGCTGCATCT CCAGGGGTCA CCTTTGCAAC TTGCTGCTGC TCCCAGGGTC
    TCGTTTAGCT CCGGGTCTCA GCTGCCCAAA ACCAATCAAG GGGGTGCCAC AGGCTTCAGT
    GCCCTCACTA CCCTTCAGCC AGACCCTCTC TCACGGCCGA ATCTTTTTTC CTGTTCTGCA
    AGTCTTCTCC CTTGTCCACT TGTAAAGTCT GTCTTTTCTT CCCCTGCCTC TCCTCCCTCC
    CTACCCAGGG GCGTTGTTCC CCCTCCTCCC CCTCTACCTG GGTGGTGTGG TGTTCTTCCT
    TCTCCCCCTC TTCCCCCTCT AACTGGGGGA CTTGTTCCCC CTCCTCCCCC TCTATCTCAG
    AGGGTTGGCA TTCCTCCTCC CCCTCCTCCC CCTCTACCTG GGTGGTGTGG TGTTCCTCCT
    TCTCCCCCAC TTCCCCCTCT AACTGGGGGA CTTGTTCCCC CTCCTCCCCC TCTACCTGGG
    TGGGGTGGTG TTCCTCCTCC TCCCCATCTT CCCCCTCTAT CTCAGGGGGT GGGCATTCCT
    CCTCCCCCTC CTCCCCCTCT TTCCTCTCCT CTTCCCTCTC ACCATCTCCC ATCTCCTAGG
    AGTGCCAGTT ATAAGCTGCC AGTGGCTCCA TCTGGCCCTG CTGAGGATGC CCGGCTGGAC
    ATTCCTCTGT TAGCTGTGTA TGATAAGCCT TTGTCATTAG ACTCCCTCCA ATATGACTGT
    TCATTTTTTG AATGTTCTTC TGAAACGGAA AGTGATAAAA CAGGAGAACT TCTGGCAAGT
    AGGATTTTTA AAAAGCTGGC AGAATATGAC CTGAGAAGTG GCTTTACTGC AAGTACTCAG
    GCACAAAAAG ATGAGGATGG CCTAGTTGGC AAAGCAAGCT GGAGGAATTA TGTGCCTTCG
    ACTGAACTCT TCAGGCTTCA GACCAAGGAT GGATGCTGGA AACTTACACA AGAACTGGGA
    TTTATATTAA AACTTAATAC AAATATTTTA AACAGCTTTC TTGAGCAAAA AGGCATTCGA
    TCTTTAGGTG TAAGAGGAAG AGAGCGCCTT CTAGATCTGA TCGCCACGTT TCTAGTGCTG
    CAGTTCCTTC GCACCAAGTT GGAACAGGAG GGAATAATCG TCAAATCGCT GATGAGACTG
    GATGATGCTT CCATTCCCAG GAATATTCCC TGGGATTGCG AGAAAATAAA GAAAGCCAGT
    GAGTGGCTCA GAAGAACTGA AGGGCAGTAC CCATCCATCT GCCAGCGGCT GGAGCTGGGT
    AAAGACTGGG ACTCCGCCAC CAAGCAGCTG CTGGGTATCC TGCCCAGAGA TGCCGCCTCC
    CCTCCTCACA GGCTCCTTAA GCACAGCCAA GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329326.1
    CDS393..5048
    Translation

    Target ORF information:

    RefSeq Version XM_015473840.1
    Organism Bos taurus(cattle)
    Definition Bos taurus poly(ADP-ribose) polymerase family member 4 (PARP4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTCTGGG CAGAAGCCCT CGGCCGCCTG GAGGACACAC TGCTGAGACC TGTGGGCATG 
    ATGAGCCTGA ATGACGTGAG CAAGGCAGAG GGAATTCTCC TCCTGGTAAA GGAAGCAATG
    AGAAATGGAG AAACACAGGA GCAACTGCAA ACGATGCTGA CCGAGTTCTA CAGATTAATC
    CCTCACAGAG CTGCTGTGGC CGAGAAGGTC AGCCTGAGCC TGCTGGCTAA GAAGGAGGAC
    CTGTGCCAGC TAATAAGAGA CATGGTTAAT GTCTGTGAAA CTAATTTGGC CAAACCCAAC
    CCCCCATCTC TTGCCAAATA CCGAGCTTTG AGATGCAAAA TTGAGCATGT TGAGCCGAAC
    ACTGAAGAAT TTTTGAGAGT AAGAGAAGAA GTGCTGCAGA ATAATCACAG TAAGGGTCCA
    GTGGACATCT TGCAGATATT TAGAGTTGGC AGAGTGAGTG AAACCACAGA GTTCCAGGGC
    CACCTTGGCA ATGTCAGGCA CTTACTGCAT GGGTCTCCTG TGCAGAGCTT CATGGGAATC
    CTGTCCCGTG GGTTGCTTTT ACCCAAAGTA GTTGAAGATC GTGGCATGAA GAGAACAGAC
    ATTGGAAATC TTGGGAGTGG GATATATTTC AGCGATTCAA TCAGTACAAG CATCAAGTAC
    TCGCACCCTG GACAGACAGA TGGCGCCCGA CTCCTGGTCG TCTGTGATGT CGCACTCGGC
    AGGTGCATGG ACCTGCACAA GCGAGACTTC TCCCTGACGG AGGCACCACC CGAGTACGAC
    AGCGTCCACG GGGTGCGGGG AACGGATATG GTCAGCTCGG ACTTTGAGGA TGATGAATTT
    GTTGTATACA AAACCAATCA GGTTAAAATG AAATATCTTG TTAAATTTTC TGTACCTGGA
    GATGAAATAA AGGACTTTCA TCCTTGTGAC AATACGGAAC TAGAGGAAGA CAGACCTGAG
    TTTTCAAATT TTTCAGCAGT TGAAGATCAC CAGCTTCCAG GCAGCACGCC TTTCCCCGAC
    ATCAAGGCCG GCCTCCAGGA TGCCTCTGGG AATTCAGTTC CTCTGGAGGA CGTTCACATC
    AAGGGGAAAA TAATAGACTT TGTAGCCCAG GTCATTGTTT TTCAAACATA CACAAACCAA
    AGTCATGTGC CCATTGAGGC AAAATATGTT TTTCCTTTGG ACGACAAGGC AGCTGTATGT
    GGTTTCGAGG CTTTCATCAA TGGGAAGCAC GTGGTGGGAG AGATTAAAGA GAAGGAAGTG
    GCCCAGCGAG AGTACCGAGT GGCGATCAGT CAAGGTCATG GCGCCTACCT GATGGATCAG
    GACACACCCG ACATCTTCAC CGTCAGCGTC GGGAACTTAC CCCCAAAGGC CAAAGTCCTG
    GTCAAAATCA CCTACATCAC GGAGCTGAGC ATCCAGGGTG CCTGCGCGGT CTTCTTCCTG
    CCGGCCACGG TGGCGCCCTG GCAGCAGGAC AGGGCTCTGA GGGAGAACCT TCAGGATACA
    GTGGAGAAGA TTTGTATAAA GGAAATAGGA GCAAAGCAAA GCTTTTCTCT GAGCATGTCT
    ATCGAGATGC CGCACATGAT TGAATTCATC TCCAGTGACA CACACGACCT GAGAAAAAAG
    AGAACAGACT GCAAAGCTGT GATTAGTACT GTGGAAGGCA GCTCCTTAGA CAGCAATGGA
    TTTTCTCTCC TCATCGGTTT GGTTGATGTG TATCTCCCAA GAATGTGGGT TGAAAAGCAT
    CCAGAAAAAG AAAGCGAGGC CTGCATGCTT GTGTTTCAAC CAAATCTTGG TATTACCGTC
    CCTGACGAAG CTGAGAGGAG TGAAGTGATT ATTTGTCTTG ATTGCTCCAA TTCCATGGAG
    GGCGAGACAT TCCTGCAAGC CAAACAGATT GCCTTGCATG CACTGTCCTG TGTGGGAAAG
    GAGCAGAAGG TGAACGTCAT CAAGTTCGGC ACAAGTTACA AGGAATTATT TTCATATCCT
    AAGTGCATCA CGAGCAATAA GATGCCGACA GAGTTCATCA TGTCTGCTAC ACCTACCATG
    GGAAACACAG ATTTCTGGAA GACACTTCGA TACTTAAATT TGCTGTACCC TTCCCACGTG
    TTACGGAACA TCCTCCTCAT ATCCGACGGG CACCTCCAGA ACGAGAGCCT GACCTTGCAG
    CTCGTAAGAA GAAGCGTGCC GCACACCAGG CTGTTCTCCT GTGGGGTCAG TTCTACAGCA
    AATCGTCATG TCTTACGGAG TCTGTCCCAC TATGGCGCTG GAGTATTTGA ATATTTTAAC
    TCAAAATCCA AACATAGCTG GAAAAAACAG ATAGAAGCCC AGATGGATAG GCTGAGTTCT
    CCCAGTTGCC ACTCGGTCTC CGTCAAATGG CAGCAGCTGA GCACGGACAC GCCCGCACCC
    CTGCAGGCCC CCACCCAGGT GCAGTCCCTG TTCCACAGCG AGCGACTCCT TGTCTACGGG
    CTCACTCCTC ACTGTACACA GGCAACTCTA CATGCGCTGA TTCAAGAGAG AGAGTATTCT
    ACAGTGGTGT CAACTACTGA ACTTCAGAAG ACAACTGGAA CGATGATTCA CAAGCTGGCA
    GCTCGAGCTC TGATAAGAGA TTATGAAGAT GGCATTCTCC ACGAAAACGA AGTGAATCAC
    GAGATGAAGA AGCAAACTTT AAAATCTCTG ATTATTAAAC TCAGTAAAGA AAACTCTCTC
    ATAACACAGT TTACAAGCTT TGTGGCTGTG GAGAAAAGGT ATGGTAATGA GTCACCTTCT
    CCTGATATTC CAAATACTTT GGAACTTATT GCCAAAGAAG ACATAGATTT TCTGCCATAC
    ATGAGCTGGG AGGGGGAACA ACCAGATGCC AGCAGGATGC AGACACTTTC AGCATCGTCT
    GAATGGAACC AAGAACCCAA ACGTAAATGT AAACGTAAAC AGACTAAAAA GAAGCGTTCA
    TCACTTAAGA TGATACTTTC TGAAGATTTT AAAGCCTTTA GCTCAGATGC CCCAGAAGAA
    TCTGAAGCGG TCAGTTTTGT CTTCAGGGAT GCAGAGGAGC CCTGGGGTTT GGGTCTGATG
    GAGTCATCTA AACAAACAGA AGTTAAACCA CAATTCACAG AAGCTATCTC ACAGAAAACG
    GCCTTTTCTG ACATTCCTAC TGCCCCTAGT TTCTTAACTA CACCCCTCCA TGTTGGCTCT
    GGTTTCTACC TTCAGGCCAG TTCAGCACTG TTTGGTTCTC CAGCTGGTCC CAAACACTTC
    GGTTCCCCTA AGAAGTACCT GGAAATGGGC AGTGGTGCCG ACGTTTCCCT GGACATGGCG
    TCTTTTCCCA GGCTGCATCT CCAGGGGTCA CCTTTGCAAC TTGCTGCTGC TCCCAGGGTC
    TCGTTTAGCT CCGGGTCTCA GCTGCCCAAA ACCAATCAAG GGGGTGCCAC AGGCTTCAGT
    GCCCTCACTA CCCTTCAGCC AGACCCTCTC TCACGGCCGA ATCTTTTTTC CTGTTCTGCA
    AGTCTTCTCC CTTGTCCACT TGTAAAGTCT GTCTTTTCTT CCCCTGCCTC TCCTCCCTCC
    CTACCCAGGG GCGTTGTTCC CCCTCCTCCC CCTCTACCTG GGTGGTGTGG TGTTCTTCCT
    TCTCCCCCTC TTCCCCCTCT AACTGGGGGA CTTGTTCCCC CTCCTCCCCC TCTATCTCAG
    AGGGTTGGCA TTCCTCCTCC CCCTCCTCCC CCTCTACCTG GGTGGTGTGG TGTTCCTCCT
    TCTCCCCCAC TTCCCCCTCT AACTGGGGGA CTTGTTCCCC CTCCTCCCCC TCTACCTGGG
    TGGGGTGGTG TTCCTCCTCC TCCCCATCTT CCCCCTCTAT CTCAGGGGGT GGGCATTCCT
    CCTCCCCCTC CTCCCCCTCT TTCCTCTCCT CTTCCCTCTC ACCATCTCCC ATCTCCTAGG
    AGTGCCAGTT ATAAGCTGCC AGTGGCTCCA TCTGGCCCTG CTGAGGATGC CCGGCTGGAC
    ATTCCTCTGT TAGCTGTGTA TGATAAGCCT TTGTCATTAG ACTCCCTCCA ATATGACTGT
    TCATTTTTTG AATGTTCTTC TGAAACGGAA AGTGATAAAA CAGGAGAACT TCTGGCAAGT
    AGGATTTTTA AAAAGCTGGC AGAATATGAC CTGAGAAGTG GCTTTACTGC AAGTACTCAG
    GCACAAAAAG ATGAGGATGG CCTAGTTGGC AAAGCAAGCT GGAGGAATTA TGTGCCTTCG
    ACTGAACTCT TCAGGCTTCA GACCAAGGAT GGATGCTGGA AACTTACACA AGAACTGGGA
    TTTATATTAA AACTTAATAC AAATATTTTA AACAGCTTTC TTGAGCAAAA AGGCATTCGA
    TCTTTAGGTG TAAGAGGAAG AGAGCGCCTT CTAGATCTGA TCGCCACGTT TCTAGTGCTG
    CAGTTCCTTC GCACCAAGTT GGAACAGGAG GGAATAATCG TCAAATCGCT GATGAGACTG
    GATGATGCTT CCATTCCCAG GAATATTCCC TGGGATTGCG AGAAAATAAA GAAAGCCAGT
    GAGTGGCTCA GAAGAACTGA AGGGCAGTAC CCATCCATCT GCCAGCGGCT GGAGCTGGGT
    AAAGACTGGG ACTCCGCCAC CAAGCAGCTG CTGGGTATCC TGCCCAGAGA TGCCGCCTCC
    CCTCCTCACA GGCTCCTTAA GCACAGCCAA GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.