PRKAR1A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKAR1A
Entrez Gene ID	615074
Full Name	protein kinase cAMP-dependent type I regulatory subunit alpha
General protein information	Preferred Names protein kinase cAMP-dependent type I regulatory subunit alpha Names cAMP-dependent protein kinase type I-alpha regulatory subunit cAMP-dependent protein kinase, regulatory subunit alpha 1 protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001076358.1	NP_001069826.1	cAMP-dependent protein kinase type I-alpha regulatory subunit
XM_024980423.1	XP_024836191.1	cAMP-dependent protein kinase type I-alpha regulatory subunit isoform X1
XM_024980422.1	XP_024836190.1	cAMP-dependent protein kinase type I-alpha regulatory subunit isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006448	XP_001688871.1
Danio rerio (zebrafish)	prkar1ab	NP_001017732.1
Drosophila melanogaster (fruit fly)	Pka-R1	NP_730573.2
Homo sapiens (human)	PRKAR1A	NP_997637.1
Pan troglodytes (chimpanzee)	PRKAR1A	XP_003315746.1
	LOC719021	XP_001112547.2
Canis lupus familiaris (dog)	PRKAR1A	XP_005624335.1
Bos taurus (cattle)	PRKAR1A	NP_001069826.1
Gallus gallus (chicken)	PRKAR1A	NP_001007846.1
Danio rerio (zebrafish)	prkar1aa	NP_001009989.1
Mus musculus (house mouse)	Prkar1a	NP_068680.1
Rattus norvegicus (Norway rat)	Prkar1a	NP_037313.1
Xenopus tropicalis (tropical clawed frog)	prkar1a	NP_001025530.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular Simulations Reveal an Unresolved Conformation of the Type IA Protein Kinase A Regulatory Subunit and Suggest Its Role in the cAMP Regulatory Mechanism.
Hirakis SP, Malmstrom RD, Amaro RE
Biochemistry56(30)3885-3888(2017 08)

An Isoform-Specific Myristylation Switch Targets Type II PKA Holoenzymes to Membranes.
Zhang P, Ye F, Bastidas AC, Kornev AP, Wu J, Ginsberg MH, Taylor SS
Structure (London, England : 1993)23(9)1563-72(2015 Sep)

Mapping the Free Energy Landscape of PKA Inhibition and Activation: A Double-Conformational Selection Model for the Tandem cAMP-Binding Domains of PKA RI?.
Akimoto M, McNicholl ET, Ramkissoon A, Moleschi K, Taylor SS, Melacini G
PLoS biology13(11)e1002305(2015)

PKA RI? homodimer structure reveals an intermolecular interface with implications for cooperative cAMP binding and Carney complex disease.
Bruystens JG, Wu J, Fortezzo A, Kornev AP, Blumenthal DK, Taylor SS
Structure (London, England : 1993)22(1)59-69(2014 Jan)

Realizing the allosteric potential of the tetrameric protein kinase A RI? holoenzyme.
Boettcher AJ, Wu J, Kim C, Yang J, Bruystens J, Cheung N, Pennypacker JK, Blumenthal DA, Kornev AP, Taylor SS
Structure (London, England : 1993)19(2)265-76(2011 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that enzyme activation by cAMP involves highly stable conformation of Prkar1a as it binds to Prkaca; glycine residue, G235, appears to function as hinge in B/C helix conserved in Prkar1a; this "Flipback" conformation plays role in cAMP association to A domain of Prkar1a. (Prkar1a = cyclic AMP-dependent protein kinase RIalpha subunit; Prkaca = cyclic AMP-dependent protein kinase catalytic subunit)
Title: Molecular Simulations Reveal an Unresolved Conformation of the Type IA Protein Kinase A Regulatory Subunit and Suggest Its Role in the cAMP Regulatory Mechanism.

Data suggest PRKAR1A contains two structurally homologous cAMP-binding domains that exhibit marked differences in dynamic profiles in activation/inhibition of Prkaca; conservation of structure does not necessarily imply conservation of dynamics.
Title: Mapping the Free Energy Landscape of PKA Inhibition and Activation: A Double-Conformational Selection Model for the Tandem cAMP-Binding Domains of PKA RI?.

Data show that RSK1 regulates PKAc activity in a cAMP-independent manner, and PKARIalpha by associating with RSK1 regulates its activation and its biological functions.
Title: p90 ribosomal S6 kinase 1 (RSK1) and the catalytic subunit of protein kinase A (PKA) compete for binding the pseudosubstrate region of PKAR1alpha: role in the regulation of PKA and RSK1 activities.

Results describe the structures of the protein kinase A RIalpha subunit D/D domain alone and in complex with D-AKAP2.
Title: Structure of D-AKAP2:PKA RI complex: insights into AKAP specificity and selectivity.

Thermodynamic analysis of protein kinase A (PKA) Ialpha activation was performed using Quantum 3.3.0 docking software and a Gaussian 03W quantum mechanical computational package.
Title: Thermodynamic analysis of protein kinase A Ialpha activation.

The following PRKAR1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKAR1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa42589	NM_001076358.1 Latest version!	Bos taurus protein kinase cAMP-dependent type I regulatory subunit alpha (PRKAR1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa226547	XM_024980422.1 Latest version!	Bos taurus protein kinase cAMP-dependent type I regulatory subunit alpha (PRKAR1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa226547	XM_024980423.1 Latest version!	Bos taurus protein kinase cAMP-dependent type I regulatory subunit alpha (PRKAR1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKAR1A ( NM_001076358.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001069826.1 Bos taurus protein kinase cAMP-dependent type I regulatory subunit alpha (PRKAR1A), mRNA.

Price & Availability↑

CloneID	OBa42589
Clone ID Related Accession (Same CDS sequence)	NM_001076358.1
Accession Version	NM_001076358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	cAMP-dependent protein kinase type I-alpha regulatory subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC118242.1. On Oct 4, 2006 this sequence version replaced XM_866779.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC118242.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145433, SAMN03145477 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTCCG GCACCACCGC CAGCGAGGAG GAGCGCAGCC TCCGGGAATG CGAGCTCTAT 
GTCCAGAAGC ACAACATCCA GGCGCTGCTC AAGGACTCCA TCGTGCAGCT GTGCACCGCA 
CGGCCCGAGA GGCCCATGGC CTTCCTCAGG GAATACTTCG AGAAGCTGGA GAAGGAGGAG 
GCAAAGCAGA TCCAGAACCT GCAGAAAGCA GGCAGCCGTG CAGACTCTCG GGAGGATGAA 
ATCTCCCCGC CGCCCCCTAA CCCGGTGGTG AAGGGCCGGC GGCGACGCGG GGCCATCAGC 
GCTGAGGTCT ACACCGAGGA GGATGCCGCG TCCTATGTTC GGAAGGTTAT ACCGAAAGAT 
TATAAGACAA TGGCTGCTTT AGCTAAAGCC ATTGAAAAGA ACGTACTGTT TTCACATCTT 
GATGATAACG AGAGAAGTGA CATTTTTGAC GCCATGTTCC CGGTTTCCTT TATTGCTGGA 
GAGACTGTTA TTCAGCAGGG TGACGAAGGG GATAACTTCT ACGTGATTGA CCAAGGAGAG 
ATGGACGTCT ATGTCAACAA TGAATGGGCA ACCAGTGTTG GGGAAGGAGG GAGCTTCGGG 
GAGCTTGCTC TGATTTACGG GACTCCTCGA GCGGCCACTG TCAAGGCCAA GACGAACGTG 
AAACTGTGGG GCATTGACCG GGACAGCTAC AGAAGGATCC TCATGGGAAG CACGCTGAGA 
AAGCGGAAGA TGTATGAGGA GTTTCTTAGT AAAGTGTCTA TTTTAGAATC TCTGGACAAG 
TGGGAGCGTC TCACGGTAGC TGATGCATTG GAACCAGTCC AGTTTGAAGA CGGGCAGAAG 
ATTGTGGTAC AGGGGGAGCC GGGTGATGAA TTCTTCATTA TTTTAGAGGG CTCAGCCGCG 
GTGCTGCAGC GGCGCTCAGA GAACGAAGAG TTTGTGGAAG TGGGAAGGTT GGGGCCTTCA 
GACTACTTCG GCGAGATCGC TCTGCTGATG AACCGGCCCC GTGCAGCCAC CGTGGTGGCC 
CGTGGCCCGC TGAAGTGCGT CAAGCTGGAC CGGCCGCGGT TCGAGCGCGT TCTCGGCCCG 
TGCTCCGACA TCCTCAAGCG CAACATCCAG CAGTACAACA GCTTCGTGTC CCTGTCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001069826.1
CDS	131..1273
Translation	MASGTTASEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFEKLEKEEAKQIQNLQKAGSRADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV

Target ORF information:

RefSeq Version	NM_001076358.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase cAMP-dependent type I regulatory subunit alpha (PRKAR1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076358.1

ORF Insert Sequence:

ATGGCTTCCG GCACCACCGC CAGCGAGGAG GAGCGCAGCC TCCGGGAATG CGAGCTCTAT 
GTCCAGAAGC ACAACATCCA GGCGCTGCTC AAGGACTCCA TCGTGCAGCT GTGCACCGCA 
CGGCCCGAGA GGCCCATGGC CTTCCTCAGG GAATACTTCG AGAAGCTGGA GAAGGAGGAG 
GCAAAGCAGA TCCAGAACCT GCAGAAAGCA GGCAGCCGTG CAGACTCTCG GGAGGATGAA 
ATCTCCCCGC CGCCCCCTAA CCCGGTGGTG AAGGGCCGGC GGCGACGCGG GGCCATCAGC 
GCTGAGGTCT ACACCGAGGA GGATGCCGCG TCCTATGTTC GGAAGGTTAT ACCGAAAGAT 
TATAAGACAA TGGCTGCTTT AGCTAAAGCC ATTGAAAAGA ACGTACTGTT TTCACATCTT 
GATGATAACG AGAGAAGTGA CATTTTTGAC GCCATGTTCC CGGTTTCCTT TATTGCTGGA 
GAGACTGTTA TTCAGCAGGG TGACGAAGGG GATAACTTCT ACGTGATTGA CCAAGGAGAG 
ATGGACGTCT ATGTCAACAA TGAATGGGCA ACCAGTGTTG GGGAAGGAGG GAGCTTCGGG 
GAGCTTGCTC TGATTTACGG GACTCCTCGA GCGGCCACTG TCAAGGCCAA GACGAACGTG 
AAACTGTGGG GCATTGACCG GGACAGCTAC AGAAGGATCC TCATGGGAAG CACGCTGAGA 
AAGCGGAAGA TGTATGAGGA GTTTCTTAGT AAAGTGTCTA TTTTAGAATC TCTGGACAAG 
TGGGAGCGTC TCACGGTAGC TGATGCATTG GAACCAGTCC AGTTTGAAGA CGGGCAGAAG 
ATTGTGGTAC AGGGGGAGCC GGGTGATGAA TTCTTCATTA TTTTAGAGGG CTCAGCCGCG 
GTGCTGCAGC GGCGCTCAGA GAACGAAGAG TTTGTGGAAG TGGGAAGGTT GGGGCCTTCA 
GACTACTTCG GCGAGATCGC TCTGCTGATG AACCGGCCCC GTGCAGCCAC CGTGGTGGCC 
CGTGGCCCGC TGAAGTGCGT CAAGCTGGAC CGGCCGCGGT TCGAGCGCGT TCTCGGCCCG 
TGCTCCGACA TCCTCAAGCG CAACATCCAG CAGTACAACA GCTTCGTGTC CCTGTCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAR1A ( XM_024980423.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836191.1 Bos taurus protein kinase cAMP-dependent type I regulatory subunit alpha (PRKAR1A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa226547
Clone ID Related Accession (Same CDS sequence)	XM_024980423.1 , XM_024980422.1
Accession Version	XM_024980423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cAMP-dependent protein kinase type I-alpha regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCG GCACCACCGC CAGCGAGGAG GAGCGCAGCC TCCGGGAATG CGAGCTCTAT 
GTCCAGAAGC ACAACATCCA GGCGCTGCTC AAGGACTCCA TCGTGCAGCT GTGCACCGCA 
CGGCCCGAGA GGCCCATGGC CTTCCTCAGG GAATACTTCG AGAAGCTGGA GAAGGAGGAG 
GCAAAGCAGA TCCAGAACCT GCAGAAAGCA GGCAGCCGTG CAGACTCTCG GGAGGATGAA 
ATCTCCCCGC CGCCCCCTAA CCCGGTGGTG AAGGGCCGGC GGCGACGCGG GGCCATCAGC 
GCTGAGGTCT ACACCGAGGA GGATGCCGCG TCCTATGTTC GGAAGGTTAT ACCGAAAGAT 
TATAAGACAA TGGCTGCTTT AGCTAAAGCC ATTGAAAAGA ACGTACTGTT TTCACATCTT 
GATGATAACG AGAGAAGTGA CATTTTTGAC GCCATGTTCC CGGTTTCCTT TATTGCTGGA 
GAGACTGTTA TTCAGCAGGG TGACGAAGGG GATAACTTCT ACGTGATTGA CCAAGGAGAG 
ATGGACGTCT ATGTCAACAA TGAATGGGCA ACCAGTGTTG GGGAAGGAGG GAGCTTCGGG 
GAGCTTGCTC TGATTTACGG GACTCCTCGA GCGGCCACTG TCAAGGCCAA GACGAACGTG 
AAACTGTGGG GCATTGACCG GGACAGCTAC AGAAGGATCC TCATGGGAAG CACGCTGAGA 
AAGCGGAAGA TGTATGAGGA GTTTCTTAGT AAAGTGTCTA TTTTAGAATC TCTGGACAAG 
TGGGAGCGTC TCACGGTAGC TGATGCATTG GAACCAGTCC AGTTTGAAGA CGGGCAGAAG 
ATTGTGGTAC AGGGGGAGCC GGGTGATGAA TTCTTCATTA TTTTAGAGGG CTCAGCCGCG 
GTGCTGCAGC GGCGCTCAGA GAACGAAGAG TTTGTGGAAG TGGGAAGGTT GGGGCCTTCA 
GACTACTTCG GCGAGATCGC TCTGCTGATG AACCGGCCCC GTGCAGCCAC CGTGGTGGCC 
CGTGGCCCGC TGAAGTGCGT CAAGCTGGAC CGGCCGCGGT TCGAGCGCGT TCTTGGCCCG 
TGCTCCGACA TCCTCAAGCG CAACATCCAG CAGTACAACA GCTTCGTGTC CCTGTCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836191.1
CDS	21..1163
Translation	MASGTTASEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFEKLEKEEAKQIQNLQKAGSRADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV

Target ORF information:

RefSeq Version	XM_024980423.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase cAMP-dependent type I regulatory subunit alpha (PRKAR1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980423.1

ORF Insert Sequence:

ATGGCTTCCG GCACCACCGC CAGCGAGGAG GAGCGCAGCC TCCGGGAATG CGAGCTCTAT 
GTCCAGAAGC ACAACATCCA GGCGCTGCTC AAGGACTCCA TCGTGCAGCT GTGCACCGCA 
CGGCCCGAGA GGCCCATGGC CTTCCTCAGG GAATACTTCG AGAAGCTGGA GAAGGAGGAG 
GCAAAGCAGA TCCAGAACCT GCAGAAAGCA GGCAGCCGTG CAGACTCTCG GGAGGATGAA 
ATCTCCCCGC CGCCCCCTAA CCCGGTGGTG AAGGGCCGGC GGCGACGCGG GGCCATCAGC 
GCTGAGGTCT ACACCGAGGA GGATGCCGCG TCCTATGTTC GGAAGGTTAT ACCGAAAGAT 
TATAAGACAA TGGCTGCTTT AGCTAAAGCC ATTGAAAAGA ACGTACTGTT TTCACATCTT 
GATGATAACG AGAGAAGTGA CATTTTTGAC GCCATGTTCC CGGTTTCCTT TATTGCTGGA 
GAGACTGTTA TTCAGCAGGG TGACGAAGGG GATAACTTCT ACGTGATTGA CCAAGGAGAG 
ATGGACGTCT ATGTCAACAA TGAATGGGCA ACCAGTGTTG GGGAAGGAGG GAGCTTCGGG 
GAGCTTGCTC TGATTTACGG GACTCCTCGA GCGGCCACTG TCAAGGCCAA GACGAACGTG 
AAACTGTGGG GCATTGACCG GGACAGCTAC AGAAGGATCC TCATGGGAAG CACGCTGAGA 
AAGCGGAAGA TGTATGAGGA GTTTCTTAGT AAAGTGTCTA TTTTAGAATC TCTGGACAAG 
TGGGAGCGTC TCACGGTAGC TGATGCATTG GAACCAGTCC AGTTTGAAGA CGGGCAGAAG 
ATTGTGGTAC AGGGGGAGCC GGGTGATGAA TTCTTCATTA TTTTAGAGGG CTCAGCCGCG 
GTGCTGCAGC GGCGCTCAGA GAACGAAGAG TTTGTGGAAG TGGGAAGGTT GGGGCCTTCA 
GACTACTTCG GCGAGATCGC TCTGCTGATG AACCGGCCCC GTGCAGCCAC CGTGGTGGCC 
CGTGGCCCGC TGAAGTGCGT CAAGCTGGAC CGGCCGCGGT TCGAGCGCGT TCTTGGCCCG 
TGCTCCGACA TCCTCAAGCG CAACATCCAG CAGTACAACA GCTTCGTGTC CCTGTCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAR1A ( XM_024980422.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836190.1 Bos taurus protein kinase cAMP-dependent type I regulatory subunit alpha (PRKAR1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226547
Clone ID Related Accession (Same CDS sequence)	XM_024980423.1 , XM_024980422.1
Accession Version	XM_024980422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cAMP-dependent protein kinase type I-alpha regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCG GCACCACCGC CAGCGAGGAG GAGCGCAGCC TCCGGGAATG CGAGCTCTAT 
GTCCAGAAGC ACAACATCCA GGCGCTGCTC AAGGACTCCA TCGTGCAGCT GTGCACCGCA 
CGGCCCGAGA GGCCCATGGC CTTCCTCAGG GAATACTTCG AGAAGCTGGA GAAGGAGGAG 
GCAAAGCAGA TCCAGAACCT GCAGAAAGCA GGCAGCCGTG CAGACTCTCG GGAGGATGAA 
ATCTCCCCGC CGCCCCCTAA CCCGGTGGTG AAGGGCCGGC GGCGACGCGG GGCCATCAGC 
GCTGAGGTCT ACACCGAGGA GGATGCCGCG TCCTATGTTC GGAAGGTTAT ACCGAAAGAT 
TATAAGACAA TGGCTGCTTT AGCTAAAGCC ATTGAAAAGA ACGTACTGTT TTCACATCTT 
GATGATAACG AGAGAAGTGA CATTTTTGAC GCCATGTTCC CGGTTTCCTT TATTGCTGGA 
GAGACTGTTA TTCAGCAGGG TGACGAAGGG GATAACTTCT ACGTGATTGA CCAAGGAGAG 
ATGGACGTCT ATGTCAACAA TGAATGGGCA ACCAGTGTTG GGGAAGGAGG GAGCTTCGGG 
GAGCTTGCTC TGATTTACGG GACTCCTCGA GCGGCCACTG TCAAGGCCAA GACGAACGTG 
AAACTGTGGG GCATTGACCG GGACAGCTAC AGAAGGATCC TCATGGGAAG CACGCTGAGA 
AAGCGGAAGA TGTATGAGGA GTTTCTTAGT AAAGTGTCTA TTTTAGAATC TCTGGACAAG 
TGGGAGCGTC TCACGGTAGC TGATGCATTG GAACCAGTCC AGTTTGAAGA CGGGCAGAAG 
ATTGTGGTAC AGGGGGAGCC GGGTGATGAA TTCTTCATTA TTTTAGAGGG CTCAGCCGCG 
GTGCTGCAGC GGCGCTCAGA GAACGAAGAG TTTGTGGAAG TGGGAAGGTT GGGGCCTTCA 
GACTACTTCG GCGAGATCGC TCTGCTGATG AACCGGCCCC GTGCAGCCAC CGTGGTGGCC 
CGTGGCCCGC TGAAGTGCGT CAAGCTGGAC CGGCCGCGGT TCGAGCGCGT TCTTGGCCCG 
TGCTCCGACA TCCTCAAGCG CAACATCCAG CAGTACAACA GCTTCGTGTC CCTGTCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836190.1
CDS	159..1301
Translation	MASGTTASEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFEKLEKEEAKQIQNLQKAGSRADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV

Target ORF information:

RefSeq Version	XM_024980422.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase cAMP-dependent type I regulatory subunit alpha (PRKAR1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980422.1

ORF Insert Sequence:

ATGGCTTCCG GCACCACCGC CAGCGAGGAG GAGCGCAGCC TCCGGGAATG CGAGCTCTAT 
GTCCAGAAGC ACAACATCCA GGCGCTGCTC AAGGACTCCA TCGTGCAGCT GTGCACCGCA 
CGGCCCGAGA GGCCCATGGC CTTCCTCAGG GAATACTTCG AGAAGCTGGA GAAGGAGGAG 
GCAAAGCAGA TCCAGAACCT GCAGAAAGCA GGCAGCCGTG CAGACTCTCG GGAGGATGAA 
ATCTCCCCGC CGCCCCCTAA CCCGGTGGTG AAGGGCCGGC GGCGACGCGG GGCCATCAGC 
GCTGAGGTCT ACACCGAGGA GGATGCCGCG TCCTATGTTC GGAAGGTTAT ACCGAAAGAT 
TATAAGACAA TGGCTGCTTT AGCTAAAGCC ATTGAAAAGA ACGTACTGTT TTCACATCTT 
GATGATAACG AGAGAAGTGA CATTTTTGAC GCCATGTTCC CGGTTTCCTT TATTGCTGGA 
GAGACTGTTA TTCAGCAGGG TGACGAAGGG GATAACTTCT ACGTGATTGA CCAAGGAGAG 
ATGGACGTCT ATGTCAACAA TGAATGGGCA ACCAGTGTTG GGGAAGGAGG GAGCTTCGGG 
GAGCTTGCTC TGATTTACGG GACTCCTCGA GCGGCCACTG TCAAGGCCAA GACGAACGTG 
AAACTGTGGG GCATTGACCG GGACAGCTAC AGAAGGATCC TCATGGGAAG CACGCTGAGA 
AAGCGGAAGA TGTATGAGGA GTTTCTTAGT AAAGTGTCTA TTTTAGAATC TCTGGACAAG 
TGGGAGCGTC TCACGGTAGC TGATGCATTG GAACCAGTCC AGTTTGAAGA CGGGCAGAAG 
ATTGTGGTAC AGGGGGAGCC GGGTGATGAA TTCTTCATTA TTTTAGAGGG CTCAGCCGCG 
GTGCTGCAGC GGCGCTCAGA GAACGAAGAG TTTGTGGAAG TGGGAAGGTT GGGGCCTTCA 
GACTACTTCG GCGAGATCGC TCTGCTGATG AACCGGCCCC GTGCAGCCAC CGTGGTGGCC 
CGTGGCCCGC TGAAGTGCGT CAAGCTGGAC CGGCCGCGGT TCGAGCGCGT TCTTGGCCCG 
TGCTCCGACA TCCTCAAGCG CAACATCCAG CAGTACAACA GCTTCGTGTC CCTGTCTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular Simulations Reveal an Unresolved Conformation of the Type IA Protein Kinase A Regulatory Subunit and Suggest Its Role in the cAMP Regulatory Mechanism.
Biochemistry56(30)3885-3888(2017 08)
Hirakis SP,Malmstrom RD,Amaro RE

An Isoform-Specific Myristylation Switch Targets Type II PKA Holoenzymes to Membranes.
Structure (London, England : 1993)23(9)1563-72(2015 Sep)
Zhang P,Ye F,Bastidas AC,Kornev AP,Wu J,Ginsberg MH,Taylor SS

Mapping the Free Energy Landscape of PKA Inhibition and Activation: A Double-Conformational Selection Model for the Tandem cAMP-Binding Domains of PKA RI?.
PLoS biology13(11)e1002305(2015)
Akimoto M,McNicholl ET,Ramkissoon A,Moleschi K,Taylor SS,Melacini G

PKA RI? homodimer structure reveals an intermolecular interface with implications for cooperative cAMP binding and Carney complex disease.
Structure (London, England : 1993)22(1)59-69(2014 Jan)
Bruystens JG,Wu J,Fortezzo A,Kornev AP,Blumenthal DK,Taylor SS

Realizing the allosteric potential of the tetrameric protein kinase A RI? holoenzyme.
Structure (London, England : 1993)19(2)265-76(2011 Feb)
Boettcher AJ,Wu J,Kim C,Yang J,Bruystens J,Cheung N,Pennypacker JK,Blumenthal DA,Kornev AP,Taylor SS

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PRKAR1A cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

ORF Insert Sequence:

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