CFAP221 cDNA ORF clone, Bos taurus(cattle)

The following CFAP221 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CFAP221 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227750 XM_024981052.1
Latest version!
Bos taurus cilia and flagella associated protein 221 (CFAP221), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa227751 XM_024981062.1
Latest version!
Bos taurus cilia and flagella associated protein 221 (CFAP221), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa227752 XM_024981066.1
Latest version!
Bos taurus cilia and flagella associated protein 221 (CFAP221), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa227753 XM_024981068.1
Latest version!
Bos taurus cilia and flagella associated protein 221 (CFAP221), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa87956 XM_015458168.1
Latest version!
Bos taurus cilia and flagella associated protein 221 (CFAP221), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa87956 XM_015462327.1
Latest version!
Bos taurus cilia and flagella associated protein 221 (CFAP221), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa227750
    Clone ID Related Accession (Same CDS sequence) XM_024981052.1
    Accession Version XM_024981052.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2514bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 221 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAGCAG TGAAAACCCC CAGCAGAGAA GTAAAGAATG CTGAAGAACC ATTTCAAAAT 
    GTGTCACCCC TCCTTTTAAG GAACCTAGTG GAGGAACCCA AGAGACCAAA AGAAGTACCC
    AATCATCTCC TAGAGTCAAA GGTTTATGCG AATCTTCTGA ATAATAAGGT CATACAGGCA
    AAACCTGGCA TATTACATTT TGGAGGCTAT CAAGTAGAAA AACAACACCA GCAAATATTG
    CATCTGGTCA ATATTTGTGA TGAAGACATA CATGTTCATA TTTTACCCCC GCAAACCAAA
    TACTTTCAGA TCAAATATGT TAAAAAGGAG CACCGCCTTG TCCCTGGCTT GTCTCTCAGG
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGATTGCAT CCGTGTTCAC
    TGTAAGGGAG ACGAGACTTT GCTTATTCCC ATTCATGCCT ATCCTGTCGT GAACACACTG
    GACTTTCCCT CATTTATAAA TCTATCAAAT GTTCTACTTG GTGAAAGCAA AAGCTACGTG
    ATTCCTTTGT GGTGCAGTTG CCCTGTGGAT TTTGAGTTTC ATGTCACTGT GGTTCATCCT
    CATCAGGCCT TTACTGTGGA GCCAACATCA GGAATAATCC CGGCGAATGG GAAGATGAAT
    GTGACAGTGA AGTTTACCCC GTTTCAGTAT GGGATGGCAC AGATAAAAGT GCAGCTGTGG
    GTTTCACAGT TCAACTCTCA ACCGTATGAG TGTGTCTTCA CCGGGACATG CTACCCCAAT
    GTGGCTTTAA AGTTGGAAGA ATTTGAAAGA GTAAATAATC TTTCTAAAAA AGTAGTTGTT
    CCTCCAGAAA AGGCAATGAC ACGTGTACCT TTTCCCCAAT TGCCAGCAAA AGCAAAGCCT
    CCAAAGATTA AGGAAATCGA ATATCACAAC TTGAGATTTC CAGTAGATTT ATTGAGTCCA
    TTTGCTGTGG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TAAAGATTAA AGAATTGAGA
    GAAGTTTTGG ACCAAGGCAC TGAGATTTCA AAAACAAGAC AAATGAAAGA AGCGCTCTTT
    GAACAGAAAG TCAGACAGGA CACTTGTGAA GAGATTGAAA ATCACCTGAA GTGGCAGGTG
    CACCTCGGTA AGGATCATAT GTCCTTTAAA TTTAAAAAAG ACATTTATGA GGAGCGGCAA
    AGAGTAAACA ACAAATACAA GCTAGATAGA GGAGATCCTA TTTTGTATGA GGAATTCCAG
    CGACGCAAGA CAGAAGTTCA CCCCAAACGG ATTTTACACG ACCTGAAAGA AAAAATCCAA
    GAATTTCATC CTAATTTTGA TCCACTCATT AATAATACTT GGGTCAACAG ATTCCGAGCA
    CAGAGACGGT TTCAACAAGC GGCTCGCAAG GTCCTGATTC ATCGCCGCTT GCTTACTATG
    CTGAATGCCG TCCGTAACAT ACAGAAAGAC ATCATTATGA AAAGGTTCAC TCAGGAAGGA
    CAGTCCATGG CACAAGACAA GAGCTTCAAA TACCACCTGG GGCGAGTCAG TAAGGACGCC
    AGCCGGTTCT CTGTGTCTCC CCAGGAAGTA CTGCCCTTTG CCTTTCCGTC CTACAGCCCT
    CCCCAGGACT CCAACGAACT GGCTCCTGGT GGCCTTGGAC CTGTCCCAAT TAAATCTTCA
    GAAATTCAAG TCAAGCAGCC CCATTCCTTC TTCAATCTGC AGGTTCCTCA GCTATACAAG
    ATCAAGAGGT ACCAGCCAGT CTCTGTGCAG GAGTCTTCAG CCAGCTACAG ACCTCAGAAG
    CTTGCTCGGG CCCTGAGGCA AGGAGCTGAG GACGAAGCCA CCACGGTCAT CACCCTGCCC
    AAGCAGGATT CCTCGCCCCA GCTGCCCGGT GAGACCTCCG TCCTGAGCAT AAAACTCCCC
    GAGGCCTTGG CCCACGCTCC AGATTACGAC CCGCTTTATA TTTTTAATCC CAGCCCGGGA
    TTGTTTGCTG TGAAGCACCC TCTGACTTAC GTGGAAACCT TGATCGATTA CCACCTGTGC
    TCCCACCCCA AGTACAAGTT CACCAAGGAG TGCCACTGCG GCTCCAGCAT CCCCATCACC
    CAGAAGCAAT TTCTCCATCA CACGGACATA ATACCTGGAG TGATGACCTG GAAGAGGTTC
    CAGAGCCTGG TGTTCTTATC CCTGCCCGAA ACTTCTAAGA CAGAGGTGTT GCCGAGCTGT
    GATTCCTTCA ACTTAGTCAT GCTGCCTGTC GACGTCCCTG CCGTTCTTGA CGCCTTGCCG
    GAAGAAGACA GGATAGAGAC AGTTGAACGT GAGCTCTGTG AACAGAGTAT GGACATTATG
    CTGACTCCAG AAATGATCGA AGCGGAATTC CCAATGCTGG ATTACAGGGA CACCAAGAAA
    GAGAAAGAGG TGAAAGACCA AGCACAGCCA ACGGAGAAGG CAGGAGAGAA GGTGCTCGAG
    GAGATGAAGA ACCTGCGGAG CAAAGCGCTC AGCTCCTACC TGATCCTAGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836820.1
    CDS355..2868
    Translation

    Target ORF information:

    RefSeq Version XM_024981052.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 221 (CFAP221), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981052.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAGCAG TGAAAACCCC CAGCAGAGAA GTAAAGAATG CTGAAGAACC ATTTCAAAAT 
    GTGTCACCCC TCCTTTTAAG GAACCTAGTG GAGGAACCCA AGAGACCAAA AGAAGTACCC
    AATCATCTCC TAGAGTCAAA GGTTTATGCG AATCTTCTGA ATAATAAGGT CATACAGGCA
    AAACCTGGCA TATTACATTT TGGAGGCTAT CAAGTAGAAA AACAACACCA GCAAATATTG
    CATCTGGTCA ATATTTGTGA TGAAGACATA CATGTTCATA TTTTACCCCC GCAAACCAAA
    TACTTTCAGA TCAAATATGT TAAAAAGGAG CACCGCCTTG TCCCTGGCTT GTCTCTCAGG
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGATTGCAT CCGTGTTCAC
    TGTAAGGGAG ACGAGACTTT GCTTATTCCC ATTCATGCCT ATCCTGTCGT GAACACACTG
    GACTTTCCCT CATTTATAAA TCTATCAAAT GTTCTACTTG GTGAAAGCAA AAGCTACGTG
    ATTCCTTTGT GGTGCAGTTG CCCTGTGGAT TTTGAGTTTC ATGTCACTGT GGTTCATCCT
    CATCAGGCCT TTACTGTGGA GCCAACATCA GGAATAATCC CGGCGAATGG GAAGATGAAT
    GTGACAGTGA AGTTTACCCC GTTTCAGTAT GGGATGGCAC AGATAAAAGT GCAGCTGTGG
    GTTTCACAGT TCAACTCTCA ACCGTATGAG TGTGTCTTCA CCGGGACATG CTACCCCAAT
    GTGGCTTTAA AGTTGGAAGA ATTTGAAAGA GTAAATAATC TTTCTAAAAA AGTAGTTGTT
    CCTCCAGAAA AGGCAATGAC ACGTGTACCT TTTCCCCAAT TGCCAGCAAA AGCAAAGCCT
    CCAAAGATTA AGGAAATCGA ATATCACAAC TTGAGATTTC CAGTAGATTT ATTGAGTCCA
    TTTGCTGTGG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TAAAGATTAA AGAATTGAGA
    GAAGTTTTGG ACCAAGGCAC TGAGATTTCA AAAACAAGAC AAATGAAAGA AGCGCTCTTT
    GAACAGAAAG TCAGACAGGA CACTTGTGAA GAGATTGAAA ATCACCTGAA GTGGCAGGTG
    CACCTCGGTA AGGATCATAT GTCCTTTAAA TTTAAAAAAG ACATTTATGA GGAGCGGCAA
    AGAGTAAACA ACAAATACAA GCTAGATAGA GGAGATCCTA TTTTGTATGA GGAATTCCAG
    CGACGCAAGA CAGAAGTTCA CCCCAAACGG ATTTTACACG ACCTGAAAGA AAAAATCCAA
    GAATTTCATC CTAATTTTGA TCCACTCATT AATAATACTT GGGTCAACAG ATTCCGAGCA
    CAGAGACGGT TTCAACAAGC GGCTCGCAAG GTCCTGATTC ATCGCCGCTT GCTTACTATG
    CTGAATGCCG TCCGTAACAT ACAGAAAGAC ATCATTATGA AAAGGTTCAC TCAGGAAGGA
    CAGTCCATGG CACAAGACAA GAGCTTCAAA TACCACCTGG GGCGAGTCAG TAAGGACGCC
    AGCCGGTTCT CTGTGTCTCC CCAGGAAGTA CTGCCCTTTG CCTTTCCGTC CTACAGCCCT
    CCCCAGGACT CCAACGAACT GGCTCCTGGT GGCCTTGGAC CTGTCCCAAT TAAATCTTCA
    GAAATTCAAG TCAAGCAGCC CCATTCCTTC TTCAATCTGC AGGTTCCTCA GCTATACAAG
    ATCAAGAGGT ACCAGCCAGT CTCTGTGCAG GAGTCTTCAG CCAGCTACAG ACCTCAGAAG
    CTTGCTCGGG CCCTGAGGCA AGGAGCTGAG GACGAAGCCA CCACGGTCAT CACCCTGCCC
    AAGCAGGATT CCTCGCCCCA GCTGCCCGGT GAGACCTCCG TCCTGAGCAT AAAACTCCCC
    GAGGCCTTGG CCCACGCTCC AGATTACGAC CCGCTTTATA TTTTTAATCC CAGCCCGGGA
    TTGTTTGCTG TGAAGCACCC TCTGACTTAC GTGGAAACCT TGATCGATTA CCACCTGTGC
    TCCCACCCCA AGTACAAGTT CACCAAGGAG TGCCACTGCG GCTCCAGCAT CCCCATCACC
    CAGAAGCAAT TTCTCCATCA CACGGACATA ATACCTGGAG TGATGACCTG GAAGAGGTTC
    CAGAGCCTGG TGTTCTTATC CCTGCCCGAA ACTTCTAAGA CAGAGGTGTT GCCGAGCTGT
    GATTCCTTCA ACTTAGTCAT GCTGCCTGTC GACGTCCCTG CCGTTCTTGA CGCCTTGCCG
    GAAGAAGACA GGATAGAGAC AGTTGAACGT GAGCTCTGTG AACAGAGTAT GGACATTATG
    CTGACTCCAG AAATGATCGA AGCGGAATTC CCAATGCTGG ATTACAGGGA CACCAAGAAA
    GAGAAAGAGG TGAAAGACCA AGCACAGCCA ACGGAGAAGG CAGGAGAGAA GGTGCTCGAG
    GAGATGAAGA ACCTGCGGAG CAAAGCGCTC AGCTCCTACC TGATCCTAGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227751
    Clone ID Related Accession (Same CDS sequence) XM_024981062.1
    Accession Version XM_024981062.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 221 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCAGCAG TGAAAACCCC CAGCAGAGAA GTAAAGAATG CTGAAGAACC ATTTCAAAAT 
    GTGTCACCCC TCCTTTTAAG GAACCTAGTG GAGGAACCCA AGAGACCAAA AGAAGTACCC
    AATCATCTCC TAGAGTCAAA GGTTTATGCG AATCTTCTGA ATAATAAGGT CATACAGGCA
    AAACCTGGCA TATTACATTT TGGAGGCTAT CAAGTAGAAA AACAACACCA GCAAATATTG
    CATCTGGTCA ATATTTGTGA TGAAGACATA CATGTTCATA TTTTACCCCC GCAAACCAAA
    TACTTTCAGA TCAAATATGT TAAAAAGGAG CACCGCCTTG TCCCTGGCTT GTCTCTCAGG
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGATTGCAT CCGTGTTCAC
    TGTAAGGGAG ACGAGACTTT GCTTATTCCC ATTCATGCCT ATCCTGTCGT GAACACACTG
    GACTTTCCCT CATTTATAAA TCTATCAAAT GTTCTACTTG GTGAAAGCAA AAGCTACGTG
    ATTCCTTTGT GGTGCAGTTG CCCTGTGGAT TTTGAGTTTC ATGTCACTGT GGTTCATCCT
    CATCAGGCCT TTACTGTGGA GCCAACATCA GGAATAATCC CGGCGAATGG GAAGATGAAT
    GTGACAGTGA AGTTTACCCC GTTTCAGTAT GGGATGGCAC AGATAAAAGT GCAGCTGTGG
    GTTTCACAGT TCAACTCTCA ACCGTATGAG TGTGTCTTCA CCGGGACATG CTACCCCAAT
    GTGGCTTTAA AGTTGGAAGA ATTTGAAAGA GTAAATAATC TTTCTAAAAA AGTAGTTGTT
    CCTCCAGAAA AGGCAATGAC ACGTGTACCT TTTCCCCAAT TGCCAGCAAA AGCAAAGCCT
    CCAAAGATTA AGGAAATCGA ATATCACAAC TTGAGATTTC CAGTAGATTT ATTGAGTCCA
    TTTGCTGTGG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TAAAGATTAA AGAATTGAGA
    GAAGTTTTGG ACCAAGGCAC TGAGATTTCA AAAACAAGAC AAATGAAAGA AGCGCTCTTT
    GAACAGAAAG TCAGACAGGA CACTTGTGAA GAGATTGAAA ATCACCTGAA GTGGCAGGTG
    CACCTCGGTA AGGATCATAT GTCCTTTAAA TTTAAAAAAG ACATTTATGA GGAGCGGCAA
    AGAGTAAACA ACAAATACAA GCTAGATAGA GGAGATCCTA TTTTGTATGA GGAATTCCAG
    CGACGCAAGA CAGAAGTTCA CCCCAAACGG ATTTTACACG ACCTGAAAGA AAAAATCCAA
    GAATTTCATC CTAATTTTGA TCCACTCATT AATAATACTT GGGTCAACAG ATTCCGAGCA
    CAGAGACGGT TTCAACAAGC GGCTCGCAAG GTCCTGATTC ATCGCCGCTT GCTTACTATG
    CTGAATGCCG TCCGTAACAT ACAGAAAGAC ATCATTATGA AAAGGTTCAC TCAGGAAGGA
    CAGTCCATGG CACAAGACAA GAGCTTCAAA TACCACCTGG GGCGAGTCAG TAAGGACGCC
    AGCCGGTTCT CTGTGTCTCC CCAGGAAGTA CTGCCCTTTG CCTTTCCGTC CTACAGCCCT
    CCCCAGGACT CCAACGAACT GGCTCCTGGT GGCCTTGGAC CTGTCCCAAT TAAATCTTCA
    GAAATTCAAG TCAAGCAGCC CCATTCCTTC TTCAATCTGC AGGTTCCTCA GCTATACAAG
    ATCAAGAGGT ACCAGCCAGT CTCTGTGCAG GAGTCTTCAG CCAGCTACAG ACCTCAGAAG
    CTTGCTCGGG CCCTGAGGCA AGGAGCTGAG GACGAAGCCA CCACGGTCAT CACCCTGCCC
    AAGCAGGATT CCTCGCCCCA GCTGCCCGGT GAGACCTCCG TCCTGAGCAT AAAACTCCCC
    GAGGCCTTGG CCCACGCTCC AGATTACGAC CCGCTTTATA TTTTTAATCC CAGCCCGGGA
    TTGTTTGCTG TGAAGCACCC TCTGACTTAC GTGGAAACCT TGATCGATTA CCACCTGTGC
    TCCCACCCCA AGTACAAGTT CACCAAGGAG TGCCACTGCG GCTCCAGCAT CCCCATCACC
    CAGAAGCAAT TTCTCCATCA CACGGACATA ATACCTGGAG TGATGACCTG GAAGAGGTTC
    CAGAGCCTGG TGTTCTTATC CCTGCCCGAA ACTTCTAAGA CAGAGGTGTT GCCGAGTGAG
    CTCTGTGAAC AGAGTATGGA CATTATGCTG ACTCCAGAAA TGATCGAAGC GGAATTCCCA
    ATGCTGGATT ACAGGGACAC CAAGAAAGAG AAAGAGGTGA AAGACCAAGC ACAGCCAACG
    GAGAAGGCAG GAGAGAAGGT GCTCGAGGAG ATGAAGAACC TGCGGAGCAA AGCGCTCAGC
    TCCTACCTGA TCCTAGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836830.1
    CDS355..2775
    Translation

    Target ORF information:

    RefSeq Version XM_024981062.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 221 (CFAP221), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCAGCAG TGAAAACCCC CAGCAGAGAA GTAAAGAATG CTGAAGAACC ATTTCAAAAT 
    GTGTCACCCC TCCTTTTAAG GAACCTAGTG GAGGAACCCA AGAGACCAAA AGAAGTACCC
    AATCATCTCC TAGAGTCAAA GGTTTATGCG AATCTTCTGA ATAATAAGGT CATACAGGCA
    AAACCTGGCA TATTACATTT TGGAGGCTAT CAAGTAGAAA AACAACACCA GCAAATATTG
    CATCTGGTCA ATATTTGTGA TGAAGACATA CATGTTCATA TTTTACCCCC GCAAACCAAA
    TACTTTCAGA TCAAATATGT TAAAAAGGAG CACCGCCTTG TCCCTGGCTT GTCTCTCAGG
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGATTGCAT CCGTGTTCAC
    TGTAAGGGAG ACGAGACTTT GCTTATTCCC ATTCATGCCT ATCCTGTCGT GAACACACTG
    GACTTTCCCT CATTTATAAA TCTATCAAAT GTTCTACTTG GTGAAAGCAA AAGCTACGTG
    ATTCCTTTGT GGTGCAGTTG CCCTGTGGAT TTTGAGTTTC ATGTCACTGT GGTTCATCCT
    CATCAGGCCT TTACTGTGGA GCCAACATCA GGAATAATCC CGGCGAATGG GAAGATGAAT
    GTGACAGTGA AGTTTACCCC GTTTCAGTAT GGGATGGCAC AGATAAAAGT GCAGCTGTGG
    GTTTCACAGT TCAACTCTCA ACCGTATGAG TGTGTCTTCA CCGGGACATG CTACCCCAAT
    GTGGCTTTAA AGTTGGAAGA ATTTGAAAGA GTAAATAATC TTTCTAAAAA AGTAGTTGTT
    CCTCCAGAAA AGGCAATGAC ACGTGTACCT TTTCCCCAAT TGCCAGCAAA AGCAAAGCCT
    CCAAAGATTA AGGAAATCGA ATATCACAAC TTGAGATTTC CAGTAGATTT ATTGAGTCCA
    TTTGCTGTGG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TAAAGATTAA AGAATTGAGA
    GAAGTTTTGG ACCAAGGCAC TGAGATTTCA AAAACAAGAC AAATGAAAGA AGCGCTCTTT
    GAACAGAAAG TCAGACAGGA CACTTGTGAA GAGATTGAAA ATCACCTGAA GTGGCAGGTG
    CACCTCGGTA AGGATCATAT GTCCTTTAAA TTTAAAAAAG ACATTTATGA GGAGCGGCAA
    AGAGTAAACA ACAAATACAA GCTAGATAGA GGAGATCCTA TTTTGTATGA GGAATTCCAG
    CGACGCAAGA CAGAAGTTCA CCCCAAACGG ATTTTACACG ACCTGAAAGA AAAAATCCAA
    GAATTTCATC CTAATTTTGA TCCACTCATT AATAATACTT GGGTCAACAG ATTCCGAGCA
    CAGAGACGGT TTCAACAAGC GGCTCGCAAG GTCCTGATTC ATCGCCGCTT GCTTACTATG
    CTGAATGCCG TCCGTAACAT ACAGAAAGAC ATCATTATGA AAAGGTTCAC TCAGGAAGGA
    CAGTCCATGG CACAAGACAA GAGCTTCAAA TACCACCTGG GGCGAGTCAG TAAGGACGCC
    AGCCGGTTCT CTGTGTCTCC CCAGGAAGTA CTGCCCTTTG CCTTTCCGTC CTACAGCCCT
    CCCCAGGACT CCAACGAACT GGCTCCTGGT GGCCTTGGAC CTGTCCCAAT TAAATCTTCA
    GAAATTCAAG TCAAGCAGCC CCATTCCTTC TTCAATCTGC AGGTTCCTCA GCTATACAAG
    ATCAAGAGGT ACCAGCCAGT CTCTGTGCAG GAGTCTTCAG CCAGCTACAG ACCTCAGAAG
    CTTGCTCGGG CCCTGAGGCA AGGAGCTGAG GACGAAGCCA CCACGGTCAT CACCCTGCCC
    AAGCAGGATT CCTCGCCCCA GCTGCCCGGT GAGACCTCCG TCCTGAGCAT AAAACTCCCC
    GAGGCCTTGG CCCACGCTCC AGATTACGAC CCGCTTTATA TTTTTAATCC CAGCCCGGGA
    TTGTTTGCTG TGAAGCACCC TCTGACTTAC GTGGAAACCT TGATCGATTA CCACCTGTGC
    TCCCACCCCA AGTACAAGTT CACCAAGGAG TGCCACTGCG GCTCCAGCAT CCCCATCACC
    CAGAAGCAAT TTCTCCATCA CACGGACATA ATACCTGGAG TGATGACCTG GAAGAGGTTC
    CAGAGCCTGG TGTTCTTATC CCTGCCCGAA ACTTCTAAGA CAGAGGTGTT GCCGAGTGAG
    CTCTGTGAAC AGAGTATGGA CATTATGCTG ACTCCAGAAA TGATCGAAGC GGAATTCCCA
    ATGCTGGATT ACAGGGACAC CAAGAAAGAG AAAGAGGTGA AAGACCAAGC ACAGCCAACG
    GAGAAGGCAG GAGAGAAGGT GCTCGAGGAG ATGAAGAACC TGCGGAGCAA AGCGCTCAGC
    TCCTACCTGA TCCTAGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227752
    Clone ID Related Accession (Same CDS sequence) XM_024981066.1
    Accession Version XM_024981066.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 221 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG TGAAAACCCC CAGCAGAGAA GTAAAGAATG CTGAAGAACC ATTTCAAAAT 
    GTGTCACCCC TCCTTTTAAG GAACCTAGTG GAGGAACCCA AGAGACCAAA AGAAGTACCC
    AATCATCTCC TAGAGTCAAA GGTTTATGCG AATCTTCTGA ATAATAAGGT CATACAGGCA
    AAACCTGGCA TATTACATTT TGGAGGCTAT CAAGTAGAAA AACAACACCA GCAAATATTG
    CATCTGGTCA ATATTTGTGA TGAAGACATA CATGTTCATA TTTTACCCCC GCAAACCAAA
    TACTTTCAGA TCAAATATGT TAAAAAGGAG CACCGCCTTG TCCCTGGCTT GTCTCTCAGG
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGATTGCAT CCGTGTTCAC
    TGTAAGGGAG ACGAGACTTT GCTTATTCCC ATTCATGCCT ATCCTGTCGT GAACACACTG
    GACTTTCCCT CATTTATAAA TCTATCAAAT GTTCTACTTG GTGAAAGCAA AAGCTACGTG
    ATTCCTTTGT GGTGCAGTTG CCCTGTGGAT TTTGAGTTTC ATGTCACTGT GGTTCATCCT
    CATCAGGCCT TTACTGTGGA GCCAACATCA GGAATAATCC CGGCGAATGG GAAGATGAAT
    GTGACAGTGA AGTTTACCCC GTTTCAGTAT GGGATGGCAC AGATAAAAGT GCAGCTGTGG
    GTTTCACAGT TCAACTCTCA ACCGTATGAG TGTGTCTTCA CCGGGACATG CTACCCCAAT
    GTGGCTTTAA AGTTGGAAGA ATTTGAAAGA GTAAATAATC TTTCTAAAAA AGTAGTTGTT
    CCTCCAGAAA AGGCAATGAC ACGTGTACCT TTTCCCCAAT TGCCAGCAAA AGCAAAGCCT
    CCAAAGATTA AGGAAATCGA ATATCACAAC TTGAGATTTC CAGTAGATTT ATTGAGTCCA
    TTTGCTGTGG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TAAAGATTAA AGAATTGAGA
    GAAGTTTTGG ACCAAGGCAC TGAGATTTCA AAAACAAGAC AAATGAAAGA AGCGCTCTTT
    GAACAGAAAG TCAGACAGGA CACTTGTGAA GAGATTGAAA ATCACCTGAA GTGGCAGGTG
    CACCTCGGTA AGGATCATAT GTCCTTTAAA TTTAAAAAAG ACATTTATGA GGAGCGGCAA
    AGAGTAAACA ACAAATACAA GCTAGATAGA GGAGATCCTA TTTTGTATGA GGAATTCCAG
    CGACGCAAGA CAGAAGTTCA CCCCAAACGG ATTTTACACG ACCTGAAAGA AAAAATCCAA
    GAATTTCATC CTAATTTTGA TCCACTCATT AATAATACTT GGGTCAACAG ATTCCGAGCA
    CAGAGACGGT TTCAACAAGC GGCTCGCAAG GTCCTGATTC ATCGCCGCTT GCTTACTATG
    CTGAATGCCG TCCGTAACAT ACAGAAAGAC ATCATTATGA AAAGGTTCAC TCAGGAAGGA
    CAGTCCATGG CACAAGACAA GAGCTTCAAA TACCACCTGG GGCGAGTCAG TAAGGACGCC
    AGCCGGTTCT CTGTGTCTCC CCAGGAAGTA CTGCCCTTTG CCTTTCCGTC CTACAGCCCT
    CCCCAGGACT CCAACGAACT GGCTCCTGGT GGCCTTGGAC CTGTCCCAAT TAAATCTTCA
    GAAATTCAAG TCAAGCAGCC CCATTCCTTC TTCAATCTGC AGGTTCCTCA GCTATACAAG
    ATCAAGAGGT ACCAGCCAGT CTCTGTGCAG GAGTCTTCAG CCAGCTACAG ACCTCAGAAG
    CTTGCTCGGG CCCTGAGGCA AGGAGCTGAG GACGAAGCCA CCACGGTCAT CACCCTGCCC
    AAGCAGGATT CCTCGCCCCA GCTGCCCGGT GAGACCTCCG TCCTGAGCAT AAAACTCCCC
    GAGGCCTTGG CCCACGCTCC AGATTACGAC CCGCTTTATA TTTTTAATCC CAGCCCGGGA
    TTGTTTGCTG TGAAGCACCC TCTGACTTAC GTGGAAACCT TGATCGATTA CCACCTGTGC
    TCCCACCCCA AGTACAAGTT CACCAAGGAG TGCCACTGCG GCTCCAGCAT CCCCATCACC
    CAGAAGCAAT TTCTCCATCA CACGGACATA ATACCTGGAG TGATGACCTG GAAGAGGTTC
    CAGAGCCTGG TGTTCTTATC CCTGCCCGAA ACTTCTAAGA CAGAGGTGTT GCCGAGGGAC
    ACAGGTTGGA TCCCTGATCT GGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836834.1
    CDS355..2601
    Translation

    Target ORF information:

    RefSeq Version XM_024981066.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 221 (CFAP221), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981066.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG TGAAAACCCC CAGCAGAGAA GTAAAGAATG CTGAAGAACC ATTTCAAAAT 
    GTGTCACCCC TCCTTTTAAG GAACCTAGTG GAGGAACCCA AGAGACCAAA AGAAGTACCC
    AATCATCTCC TAGAGTCAAA GGTTTATGCG AATCTTCTGA ATAATAAGGT CATACAGGCA
    AAACCTGGCA TATTACATTT TGGAGGCTAT CAAGTAGAAA AACAACACCA GCAAATATTG
    CATCTGGTCA ATATTTGTGA TGAAGACATA CATGTTCATA TTTTACCCCC GCAAACCAAA
    TACTTTCAGA TCAAATATGT TAAAAAGGAG CACCGCCTTG TCCCTGGCTT GTCTCTCAGG
    ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGATTGCAT CCGTGTTCAC
    TGTAAGGGAG ACGAGACTTT GCTTATTCCC ATTCATGCCT ATCCTGTCGT GAACACACTG
    GACTTTCCCT CATTTATAAA TCTATCAAAT GTTCTACTTG GTGAAAGCAA AAGCTACGTG
    ATTCCTTTGT GGTGCAGTTG CCCTGTGGAT TTTGAGTTTC ATGTCACTGT GGTTCATCCT
    CATCAGGCCT TTACTGTGGA GCCAACATCA GGAATAATCC CGGCGAATGG GAAGATGAAT
    GTGACAGTGA AGTTTACCCC GTTTCAGTAT GGGATGGCAC AGATAAAAGT GCAGCTGTGG
    GTTTCACAGT TCAACTCTCA ACCGTATGAG TGTGTCTTCA CCGGGACATG CTACCCCAAT
    GTGGCTTTAA AGTTGGAAGA ATTTGAAAGA GTAAATAATC TTTCTAAAAA AGTAGTTGTT
    CCTCCAGAAA AGGCAATGAC ACGTGTACCT TTTCCCCAAT TGCCAGCAAA AGCAAAGCCT
    CCAAAGATTA AGGAAATCGA ATATCACAAC TTGAGATTTC CAGTAGATTT ATTGAGTCCA
    TTTGCTGTGG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TAAAGATTAA AGAATTGAGA
    GAAGTTTTGG ACCAAGGCAC TGAGATTTCA AAAACAAGAC AAATGAAAGA AGCGCTCTTT
    GAACAGAAAG TCAGACAGGA CACTTGTGAA GAGATTGAAA ATCACCTGAA GTGGCAGGTG
    CACCTCGGTA AGGATCATAT GTCCTTTAAA TTTAAAAAAG ACATTTATGA GGAGCGGCAA
    AGAGTAAACA ACAAATACAA GCTAGATAGA GGAGATCCTA TTTTGTATGA GGAATTCCAG
    CGACGCAAGA CAGAAGTTCA CCCCAAACGG ATTTTACACG ACCTGAAAGA AAAAATCCAA
    GAATTTCATC CTAATTTTGA TCCACTCATT AATAATACTT GGGTCAACAG ATTCCGAGCA
    CAGAGACGGT TTCAACAAGC GGCTCGCAAG GTCCTGATTC ATCGCCGCTT GCTTACTATG
    CTGAATGCCG TCCGTAACAT ACAGAAAGAC ATCATTATGA AAAGGTTCAC TCAGGAAGGA
    CAGTCCATGG CACAAGACAA GAGCTTCAAA TACCACCTGG GGCGAGTCAG TAAGGACGCC
    AGCCGGTTCT CTGTGTCTCC CCAGGAAGTA CTGCCCTTTG CCTTTCCGTC CTACAGCCCT
    CCCCAGGACT CCAACGAACT GGCTCCTGGT GGCCTTGGAC CTGTCCCAAT TAAATCTTCA
    GAAATTCAAG TCAAGCAGCC CCATTCCTTC TTCAATCTGC AGGTTCCTCA GCTATACAAG
    ATCAAGAGGT ACCAGCCAGT CTCTGTGCAG GAGTCTTCAG CCAGCTACAG ACCTCAGAAG
    CTTGCTCGGG CCCTGAGGCA AGGAGCTGAG GACGAAGCCA CCACGGTCAT CACCCTGCCC
    AAGCAGGATT CCTCGCCCCA GCTGCCCGGT GAGACCTCCG TCCTGAGCAT AAAACTCCCC
    GAGGCCTTGG CCCACGCTCC AGATTACGAC CCGCTTTATA TTTTTAATCC CAGCCCGGGA
    TTGTTTGCTG TGAAGCACCC TCTGACTTAC GTGGAAACCT TGATCGATTA CCACCTGTGC
    TCCCACCCCA AGTACAAGTT CACCAAGGAG TGCCACTGCG GCTCCAGCAT CCCCATCACC
    CAGAAGCAAT TTCTCCATCA CACGGACATA ATACCTGGAG TGATGACCTG GAAGAGGTTC
    CAGAGCCTGG TGTTCTTATC CCTGCCCGAA ACTTCTAAGA CAGAGGTGTT GCCGAGGGAC
    ACAGGTTGGA TCCCTGATCT GGGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227753
    Clone ID Related Accession (Same CDS sequence) XM_024981068.1
    Accession Version XM_024981068.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 221 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGTGGCG ATACTATTAT GATTGCATCC GTGTTCACTG GAGACGAGAC TTTGCTTATT 
    CCCATTCATG CCTATCCTGT CGTGAACACA CTGGACTTTC CCTCATTTAT AAATCTATCA
    AATGTTCTAC TTGGTGAAAG CAAAAGCTAC GTGATTCCTT TGTGGTGCAG TTGCCCTGTG
    GATTTTGAGT TTCATGTCAC TGTGGTTCAT CCTCATCAGG CCTTTACTGT GGAGCCAACA
    TCAGGAATAA TCCCGGCGAA TGGGAAGATG AATGTGACAG TGAAGTTTAC CCCGTTTCAG
    TATGGGATGG CACAGATAAA AGTGCAGCTG TGGGTTTCAC AGTTCAACTC TCAACCGTAT
    GAGTGTGTCT TCACCGGGAC ATGCTACCCC AATGTGGCTT TAAAGTTGGA AGAATTTGAA
    AGAGTAAATA ATCTTTCTAA AAAAGTAGTT GTTCCTCCAG AAAAGGCAAT GACACGTGTA
    CCTTTTCCCC AATTGCCAGC AAAAGCAAAG CCTCCAAAGA TTAAGGAAAT CGAATATCAC
    AACTTGAGAT TTCCAGTAGA TTTATTGAGT CCATTTGCTG TGGCAACTGT TTTAAACCAA
    GAACCAGGAA AATTAAAGAT TAAAGAATTG AGAGAAGTTT TGGACCAAGG CACTGAGATT
    TCAAAAACAA GACAAATGAA AGAAGCGCTC TTTGAACAGA AAGTCAGACA GGACACTTGT
    GAAGAGATTG AAAATCACCT GAAGTGGCAG GTGCACCTCG GTAAGGATCA TATGTCCTTT
    AAATTTAAAA AAGACATTTA TGAGGAGCGG CAAAGAGTAA ACAACAAATA CAAGCTAGAT
    AGAGGAGATC CTATTTTGTA TGAGGAATTC CAGCGACGCA AGACAGAAGT TCACCCCAAA
    CGGATTTTAC ACGACCTGAA AGAAAAAATC CAAGAATTTC ATCCTAATTT TGATCCACTC
    ATTAATAATA CTTGGGTCAA CAGATTCCGA GCACAGAGAC GGTTTCAACA AGCGGCTCGC
    AAGGTCCTGA TTCATCGCCG CTTGCTTACT ATGCTGAATG CCGTCCGTAA CATACAGAAA
    GACATCATTA TGAAAAGGTT CACTCAGGAA GGACAGTCCA TGGCACAAGA CAAGAGCTTC
    AAATACCACC TGGGGCGAGT CAGTAAGGAC GCCAGCCGGT TCTCTGTGTC TCCCCAGGAA
    GTACTGCCCT TTGCCTTTCC GTCCTACAGC CCTCCCCAGG ACTCCAACGA ACTGGCTCCT
    GGTGGCCTTG GACCTGTCCC AATTAAATCT TCAGAAATTC AAGTCAAGCA GCCCCATTCC
    TTCTTCAATC TGCAGGTTCC TCAGCTATAC AAGATCAAGA GGTACCAGCC AGTCTCTGTG
    CAGGAGTCTT CAGCCAGCTA CAGACCTCAG AAGCTTGCTC GGGCCCTGAG GCAAGGAGCT
    GAGGACGAAG CCACCACGGT CATCACCCTG CCCAAGCAGG ATTCCTCGCC CCAGCTGCCC
    GGTGAGACCT CCGTCCTGAG CATAAAACTC CCCGAGGCCT TGGCCCACGC TCCAGATTAC
    GACCCGCTTT ATATTTTTAA TCCCAGCCCG GGATTGTTTG CTGTGAAGCA CCCTCTGACT
    TACGTGGAAA CCTTGATCGA TTACCACCTG TGCTCCCACC CCAAGTACAA GTTCACCAAG
    GAGTGCCACT GCGGCTCCAG CATCCCCATC ACCCAGAAGC AATTTCTCCA TCACACGGAC
    ATAATACCTG GAGTGATGAC CTGGAAGAGG TTCCAGAGCC TGGTGTTCTT ATCCCTGCCC
    GAAACTTCTA AGACAGAGGT GTTGCCGAGC TGTGATTCCT TCAACTTAGT CATGCTGCCT
    GTCGACGTCC CTGCCGTTCT TGACGCCTTG CCGGAAGAAG ACAGGATAGA GACAGTTGAA
    CGTGAGCTCT GTGAACAGAG TATGGACATT ATGCTGACTC CAGAAATGAT CGAAGCGGAA
    TTCCCAATGC TGGATTACAG GGACACCAAG AAAGAGAAAG AGGTGAAAGA CCAAGCACAG
    CCAACGGAGA AGGCAGGAGA GAAGGTGCTC GAGGAGATGA AGAACCTGCG GAGCAAAGCG
    CTCAGCTCCT ACCTGATCCT AGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836836.1
    CDS55..2181
    Translation

    Target ORF information:

    RefSeq Version XM_024981068.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 221 (CFAP221), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981068.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGTGGCG ATACTATTAT GATTGCATCC GTGTTCACTG GAGACGAGAC TTTGCTTATT 
    CCCATTCATG CCTATCCTGT CGTGAACACA CTGGACTTTC CCTCATTTAT AAATCTATCA
    AATGTTCTAC TTGGTGAAAG CAAAAGCTAC GTGATTCCTT TGTGGTGCAG TTGCCCTGTG
    GATTTTGAGT TTCATGTCAC TGTGGTTCAT CCTCATCAGG CCTTTACTGT GGAGCCAACA
    TCAGGAATAA TCCCGGCGAA TGGGAAGATG AATGTGACAG TGAAGTTTAC CCCGTTTCAG
    TATGGGATGG CACAGATAAA AGTGCAGCTG TGGGTTTCAC AGTTCAACTC TCAACCGTAT
    GAGTGTGTCT TCACCGGGAC ATGCTACCCC AATGTGGCTT TAAAGTTGGA AGAATTTGAA
    AGAGTAAATA ATCTTTCTAA AAAAGTAGTT GTTCCTCCAG AAAAGGCAAT GACACGTGTA
    CCTTTTCCCC AATTGCCAGC AAAAGCAAAG CCTCCAAAGA TTAAGGAAAT CGAATATCAC
    AACTTGAGAT TTCCAGTAGA TTTATTGAGT CCATTTGCTG TGGCAACTGT TTTAAACCAA
    GAACCAGGAA AATTAAAGAT TAAAGAATTG AGAGAAGTTT TGGACCAAGG CACTGAGATT
    TCAAAAACAA GACAAATGAA AGAAGCGCTC TTTGAACAGA AAGTCAGACA GGACACTTGT
    GAAGAGATTG AAAATCACCT GAAGTGGCAG GTGCACCTCG GTAAGGATCA TATGTCCTTT
    AAATTTAAAA AAGACATTTA TGAGGAGCGG CAAAGAGTAA ACAACAAATA CAAGCTAGAT
    AGAGGAGATC CTATTTTGTA TGAGGAATTC CAGCGACGCA AGACAGAAGT TCACCCCAAA
    CGGATTTTAC ACGACCTGAA AGAAAAAATC CAAGAATTTC ATCCTAATTT TGATCCACTC
    ATTAATAATA CTTGGGTCAA CAGATTCCGA GCACAGAGAC GGTTTCAACA AGCGGCTCGC
    AAGGTCCTGA TTCATCGCCG CTTGCTTACT ATGCTGAATG CCGTCCGTAA CATACAGAAA
    GACATCATTA TGAAAAGGTT CACTCAGGAA GGACAGTCCA TGGCACAAGA CAAGAGCTTC
    AAATACCACC TGGGGCGAGT CAGTAAGGAC GCCAGCCGGT TCTCTGTGTC TCCCCAGGAA
    GTACTGCCCT TTGCCTTTCC GTCCTACAGC CCTCCCCAGG ACTCCAACGA ACTGGCTCCT
    GGTGGCCTTG GACCTGTCCC AATTAAATCT TCAGAAATTC AAGTCAAGCA GCCCCATTCC
    TTCTTCAATC TGCAGGTTCC TCAGCTATAC AAGATCAAGA GGTACCAGCC AGTCTCTGTG
    CAGGAGTCTT CAGCCAGCTA CAGACCTCAG AAGCTTGCTC GGGCCCTGAG GCAAGGAGCT
    GAGGACGAAG CCACCACGGT CATCACCCTG CCCAAGCAGG ATTCCTCGCC CCAGCTGCCC
    GGTGAGACCT CCGTCCTGAG CATAAAACTC CCCGAGGCCT TGGCCCACGC TCCAGATTAC
    GACCCGCTTT ATATTTTTAA TCCCAGCCCG GGATTGTTTG CTGTGAAGCA CCCTCTGACT
    TACGTGGAAA CCTTGATCGA TTACCACCTG TGCTCCCACC CCAAGTACAA GTTCACCAAG
    GAGTGCCACT GCGGCTCCAG CATCCCCATC ACCCAGAAGC AATTTCTCCA TCACACGGAC
    ATAATACCTG GAGTGATGAC CTGGAAGAGG TTCCAGAGCC TGGTGTTCTT ATCCCTGCCC
    GAAACTTCTA AGACAGAGGT GTTGCCGAGC TGTGATTCCT TCAACTTAGT CATGCTGCCT
    GTCGACGTCC CTGCCGTTCT TGACGCCTTG CCGGAAGAAG ACAGGATAGA GACAGTTGAA
    CGTGAGCTCT GTGAACAGAG TATGGACATT ATGCTGACTC CAGAAATGAT CGAAGCGGAA
    TTCCCAATGC TGGATTACAG GGACACCAAG AAAGAGAAAG AGGTGAAAGA CCAAGCACAG
    CCAACGGAGA AGGCAGGAGA GAAGGTGCTC GAGGAGATGA AGAACCTGCG GAGCAAAGCG
    CTCAGCTCCT ACCTGATCCT AGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87956
    Clone ID Related Accession (Same CDS sequence) XM_015458168.1 , XM_015462327.1
    Accession Version XM_015458168.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 221
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103843.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCGGCACA TCCAGCCTCT GATAAAGCAA TGTGTTCATG TTAATGCTTC TTTTTCTCTC 
    CAGCATCTGG TCAATATTTG TGATGAAGAC ATACATGTTC ATATTTTACC CCCGCAAACC
    AAATACTTTC AGATCAAATA TGTTAAAAAG GAGCACCGCC TTGTCCCTGG CTTGTCTCTC
    AGGATCACCA TTACATTTTC TCCAGATGAG TGGCGATACT ATTATGATTG CATCCGTGTT
    CACTGTAAGG GAGACGAGAC TTTGCTTATT CCCATTCATG CCTATCCTGT CGTGAACACA
    CTGGACTTTC CCTCATTTAT AAATCTATCA AATGTTCTAC TTGGTGAAAG CAAAAGCTAC
    GTGATTCCTT TGTGGTGCAG TTGCCCTGTG GATTTTGAGT TTCATGTCAC TGTGGTTCAT
    CCTCATCAGG CCTTTACTGT GGAGCCAACA TCAGGAATAA TCCCGGCGAA TGGGAAGATG
    AATGTGACAG TGAAGTTTAC CCCGTTTCAG TATGGGATGG CACAGATAAA AGTGCAGCTG
    TGGGTTTCAC AGTTCAACTC TCAACCGTAT GAGTGTGTCT TCACCGGGAC ATGCTACCCC
    AATGTGGCTT TAAAGTTGGA AGAATTTGAA AGAGTAAATA ATCTTTCTAA AAAAGTAGTT
    GTTCCTCCAG AAAAGGCAAT GACACGTGTA CCTTTTCCCC AATTGCCAGC AAAAGCAAAG
    CCTCCAAAGA TTAAGGAAAT CGAATATCAC AACTTGAGAT TTCCAGTAGA TTTATTGAGT
    CCATTTGCTG TGGCAACTGT TTTAAACCAA GAACCAGGAA AATTAAAGAT TAAAGAATTG
    AGAGAAGTTT TGGACCAAGG CACTGAGATT TCAAAAACAA GACAAATGAA AGAAGCGCTC
    TTTGAACAGA AAGTCAGACA GGACACTTGT GAAGAGATTG AAAATCACCT GAAGTGGCAG
    GTGCACCTCG GTAAGGATCA TATGTCCTTT AAATTTAAAA AAGACATTTA TGAGGAGCGG
    CAAAGAGTAA ACAACAAATA CAAGCTAGAT AGAGGAGATC CTATTTTGTA TGAGGAATTC
    CAGCGACGCA AGACAGAAGT TCACCCCAAA CGGATTTTAC ACGACCTGAA AGAAAAAATC
    CAAGAATTTC ATCCTAATTT TGATCCACTC ATTAATAATA CTTGGGTCAA CAGATTCCGA
    GCACAGAGAC GGTTTCAACA AGCGGCTCGC AAGGTCCTGA TTCATCGCCG CTTGCTTACT
    ATGCTGAATG CCGTCCGTAA CATACAGAAA GACATCATTA TGAAAAGGTT CACTCAGGAA
    GGACAGTCCA TGGCACAAGA CAAGAGCTTC AAATACCACC TGGGGCGAGT CAGTAAGGAC
    GCCAGCCGGT TCTCTGTGTC TCCCCAGGAA GTACTGCCCT TTGCCTTTCC GTCCTACAGC
    CCTCCCCAGG ACTCCAACGA ACTGGCTCCT GGTGGCCTTG GACCTGTCCC AATTAAATCT
    TCAGAAATTC AAGTCAAGCA GCCCCATTCC TTCTTCAATC TGCAGGTTCC TCAGCTATAC
    AAGATCAAGA GGTACCAGCC AGTCTCTGTG CAGGAGTCTT CAGCCAGCTA CAGACCTCAG
    AAGCTTGCTC GGGCCCTGAG GCAAGGAGCT GAGGACGAAG CCACCACGGT CATCACCCTG
    CCCAAGCAGG ATTCCTCGCC CCAGCTGCCC GGTGAGACCT CCGTCCTGAG CATAAAACTC
    CCCGAGGCCT TGGCCCACGC TCCAGATTAC GACCCGCTTT ATATTTTTAA TCCCAGCCCG
    GGATTGTTTG CTGTGAAGCA CCCTCTGACT TACGTGGAAA CCTTGATCGA TTACCACCTG
    TGCTCCCACC CCAAGTACAA GTTCACCAAG GAGTGCCACT GCGGCTCCAG CATCCCCATC
    ACCCAGAAGC AATTTCTCCA TCACACGGAC ATAATACCTG GAGTGATGAC CTGGAAGAGG
    TTCCAGAGCC TGGTGTTCTT ATCCCTGCCC GAAACTTCTA AGACAGAGGT GTTGCCGAGC
    TGTGATTCCT TCAACTTAGT CATGCTGCCT GTCGACGTCC CTGCCGTTCT TGACGCCTTG
    CCGGAAGAAG ACAGGATAGA GACAGTTGAA CGTGAGCTCT GTGAACAGAG TATGGACATT
    ATGCTGACTC CAGAAATGAT CGAAGCGGAA TTCCCAATGC TGGATTACAG GGACACCAAG
    AAAGAGAAAG AGGTGAAAGA CCAAGCACAG CCAACGGAGA AGGCAGGAGA GAAGGTGCTC
    GAGGAGATGA AGAACCTGCG GAGCAAAGCG CTCAGCTCCT ACCTGATCCT AGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015313654.1
    CDS1..2337
    Translation

    Target ORF information:

    RefSeq Version XM_015458168.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 221 (CFAP221), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458168.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCGGCACA TCCAGCCTCT GATAAAGCAA TGTGTTCATG TTAATGCTTC TTTTTCTCTC 
    CAGCATCTGG TCAATATTTG TGATGAAGAC ATACATGTTC ATATTTTACC CCCGCAAACC
    AAATACTTTC AGATCAAATA TGTTAAAAAG GAGCACCGCC TTGTCCCTGG CTTGTCTCTC
    AGGATCACCA TTACATTTTC TCCAGATGAG TGGCGATACT ATTATGATTG CATCCGTGTT
    CACTGTAAGG GAGACGAGAC TTTGCTTATT CCCATTCATG CCTATCCTGT CGTGAACACA
    CTGGACTTTC CCTCATTTAT AAATCTATCA AATGTTCTAC TTGGTGAAAG CAAAAGCTAC
    GTGATTCCTT TGTGGTGCAG TTGCCCTGTG GATTTTGAGT TTCATGTCAC TGTGGTTCAT
    CCTCATCAGG CCTTTACTGT GGAGCCAACA TCAGGAATAA TCCCGGCGAA TGGGAAGATG
    AATGTGACAG TGAAGTTTAC CCCGTTTCAG TATGGGATGG CACAGATAAA AGTGCAGCTG
    TGGGTTTCAC AGTTCAACTC TCAACCGTAT GAGTGTGTCT TCACCGGGAC ATGCTACCCC
    AATGTGGCTT TAAAGTTGGA AGAATTTGAA AGAGTAAATA ATCTTTCTAA AAAAGTAGTT
    GTTCCTCCAG AAAAGGCAAT GACACGTGTA CCTTTTCCCC AATTGCCAGC AAAAGCAAAG
    CCTCCAAAGA TTAAGGAAAT CGAATATCAC AACTTGAGAT TTCCAGTAGA TTTATTGAGT
    CCATTTGCTG TGGCAACTGT TTTAAACCAA GAACCAGGAA AATTAAAGAT TAAAGAATTG
    AGAGAAGTTT TGGACCAAGG CACTGAGATT TCAAAAACAA GACAAATGAA AGAAGCGCTC
    TTTGAACAGA AAGTCAGACA GGACACTTGT GAAGAGATTG AAAATCACCT GAAGTGGCAG
    GTGCACCTCG GTAAGGATCA TATGTCCTTT AAATTTAAAA AAGACATTTA TGAGGAGCGG
    CAAAGAGTAA ACAACAAATA CAAGCTAGAT AGAGGAGATC CTATTTTGTA TGAGGAATTC
    CAGCGACGCA AGACAGAAGT TCACCCCAAA CGGATTTTAC ACGACCTGAA AGAAAAAATC
    CAAGAATTTC ATCCTAATTT TGATCCACTC ATTAATAATA CTTGGGTCAA CAGATTCCGA
    GCACAGAGAC GGTTTCAACA AGCGGCTCGC AAGGTCCTGA TTCATCGCCG CTTGCTTACT
    ATGCTGAATG CCGTCCGTAA CATACAGAAA GACATCATTA TGAAAAGGTT CACTCAGGAA
    GGACAGTCCA TGGCACAAGA CAAGAGCTTC AAATACCACC TGGGGCGAGT CAGTAAGGAC
    GCCAGCCGGT TCTCTGTGTC TCCCCAGGAA GTACTGCCCT TTGCCTTTCC GTCCTACAGC
    CCTCCCCAGG ACTCCAACGA ACTGGCTCCT GGTGGCCTTG GACCTGTCCC AATTAAATCT
    TCAGAAATTC AAGTCAAGCA GCCCCATTCC TTCTTCAATC TGCAGGTTCC TCAGCTATAC
    AAGATCAAGA GGTACCAGCC AGTCTCTGTG CAGGAGTCTT CAGCCAGCTA CAGACCTCAG
    AAGCTTGCTC GGGCCCTGAG GCAAGGAGCT GAGGACGAAG CCACCACGGT CATCACCCTG
    CCCAAGCAGG ATTCCTCGCC CCAGCTGCCC GGTGAGACCT CCGTCCTGAG CATAAAACTC
    CCCGAGGCCT TGGCCCACGC TCCAGATTAC GACCCGCTTT ATATTTTTAA TCCCAGCCCG
    GGATTGTTTG CTGTGAAGCA CCCTCTGACT TACGTGGAAA CCTTGATCGA TTACCACCTG
    TGCTCCCACC CCAAGTACAA GTTCACCAAG GAGTGCCACT GCGGCTCCAG CATCCCCATC
    ACCCAGAAGC AATTTCTCCA TCACACGGAC ATAATACCTG GAGTGATGAC CTGGAAGAGG
    TTCCAGAGCC TGGTGTTCTT ATCCCTGCCC GAAACTTCTA AGACAGAGGT GTTGCCGAGC
    TGTGATTCCT TCAACTTAGT CATGCTGCCT GTCGACGTCC CTGCCGTTCT TGACGCCTTG
    CCGGAAGAAG ACAGGATAGA GACAGTTGAA CGTGAGCTCT GTGAACAGAG TATGGACATT
    ATGCTGACTC CAGAAATGAT CGAAGCGGAA TTCCCAATGC TGGATTACAG GGACACCAAG
    AAAGAGAAAG AGGTGAAAGA CCAAGCACAG CCAACGGAGA AGGCAGGAGA GAAGGTGCTC
    GAGGAGATGA AGAACCTGCG GAGCAAAGCG CTCAGCTCCT ACCTGATCCT AGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87956
    Clone ID Related Accession (Same CDS sequence) XM_015458168.1 , XM_015462327.1
    Accession Version XM_015462327.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 221
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644601.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCGGCACA TCCAGCCTCT GATAAAGCAA TGTGTTCATG TTAATGCTTC TTTTTCTCTC 
    CAGCATCTGG TCAATATTTG TGATGAAGAC ATACATGTTC ATATTTTACC CCCGCAAACC
    AAATACTTTC AGATCAAATA TGTTAAAAAG GAGCACCGCC TTGTCCCTGG CTTGTCTCTC
    AGGATCACCA TTACATTTTC TCCAGATGAG TGGCGATACT ATTATGATTG CATCCGTGTT
    CACTGTAAGG GAGACGAGAC TTTGCTTATT CCCATTCATG CCTATCCTGT CGTGAACACA
    CTGGACTTTC CCTCATTTAT AAATCTATCA AATGTTCTAC TTGGTGAAAG CAAAAGCTAC
    GTGATTCCTT TGTGGTGCAG TTGCCCTGTG GATTTTGAGT TTCATGTCAC TGTGGTTCAT
    CCTCATCAGG CCTTTACTGT GGAGCCAACA TCAGGAATAA TCCCGGCGAA TGGGAAGATG
    AATGTGACAG TGAAGTTTAC CCCGTTTCAG TATGGGATGG CACAGATAAA AGTGCAGCTG
    TGGGTTTCAC AGTTCAACTC TCAACCGTAT GAGTGTGTCT TCACCGGGAC ATGCTACCCC
    AATGTGGCTT TAAAGTTGGA AGAATTTGAA AGAGTAAATA ATCTTTCTAA AAAAGTAGTT
    GTTCCTCCAG AAAAGGCAAT GACACGTGTA CCTTTTCCCC AATTGCCAGC AAAAGCAAAG
    CCTCCAAAGA TTAAGGAAAT CGAATATCAC AACTTGAGAT TTCCAGTAGA TTTATTGAGT
    CCATTTGCTG TGGCAACTGT TTTAAACCAA GAACCAGGAA AATTAAAGAT TAAAGAATTG
    AGAGAAGTTT TGGACCAAGG CACTGAGATT TCAAAAACAA GACAAATGAA AGAAGCGCTC
    TTTGAACAGA AAGTCAGACA GGACACTTGT GAAGAGATTG AAAATCACCT GAAGTGGCAG
    GTGCACCTCG GTAAGGATCA TATGTCCTTT AAATTTAAAA AAGACATTTA TGAGGAGCGG
    CAAAGAGTAA ACAACAAATA CAAGCTAGAT AGAGGAGATC CTATTTTGTA TGAGGAATTC
    CAGCGACGCA AGACAGAAGT TCACCCCAAA CGGATTTTAC ACGACCTGAA AGAAAAAATC
    CAAGAATTTC ATCCTAATTT TGATCCACTC ATTAATAATA CTTGGGTCAA CAGATTCCGA
    GCACAGAGAC GGTTTCAACA AGCGGCTCGC AAGGTCCTGA TTCATCGCCG CTTGCTTACT
    ATGCTGAATG CCGTCCGTAA CATACAGAAA GACATCATTA TGAAAAGGTT CACTCAGGAA
    GGACAGTCCA TGGCACAAGA CAAGAGCTTC AAATACCACC TGGGGCGAGT CAGTAAGGAC
    GCCAGCCGGT TCTCTGTGTC TCCCCAGGAA GTACTGCCCT TTGCCTTTCC GTCCTACAGC
    CCTCCCCAGG ACTCCAACGA ACTGGCTCCT GGTGGCCTTG GACCTGTCCC AATTAAATCT
    TCAGAAATTC AAGTCAAGCA GCCCCATTCC TTCTTCAATC TGCAGGTTCC TCAGCTATAC
    AAGATCAAGA GGTACCAGCC AGTCTCTGTG CAGGAGTCTT CAGCCAGCTA CAGACCTCAG
    AAGCTTGCTC GGGCCCTGAG GCAAGGAGCT GAGGACGAAG CCACCACGGT CATCACCCTG
    CCCAAGCAGG ATTCCTCGCC CCAGCTGCCC GGTGAGACCT CCGTCCTGAG CATAAAACTC
    CCCGAGGCCT TGGCCCACGC TCCAGATTAC GACCCGCTTT ATATTTTTAA TCCCAGCCCG
    GGATTGTTTG CTGTGAAGCA CCCTCTGACT TACGTGGAAA CCTTGATCGA TTACCACCTG
    TGCTCCCACC CCAAGTACAA GTTCACCAAG GAGTGCCACT GCGGCTCCAG CATCCCCATC
    ACCCAGAAGC AATTTCTCCA TCACACGGAC ATAATACCTG GAGTGATGAC CTGGAAGAGG
    TTCCAGAGCC TGGTGTTCTT ATCCCTGCCC GAAACTTCTA AGACAGAGGT GTTGCCGAGC
    TGTGATTCCT TCAACTTAGT CATGCTGCCT GTCGACGTCC CTGCCGTTCT TGACGCCTTG
    CCGGAAGAAG ACAGGATAGA GACAGTTGAA CGTGAGCTCT GTGAACAGAG TATGGACATT
    ATGCTGACTC CAGAAATGAT CGAAGCGGAA TTCCCAATGC TGGATTACAG GGACACCAAG
    AAAGAGAAAG AGGTGAAAGA CCAAGCACAG CCAACGGAGA AGGCAGGAGA GAAGGTGCTC
    GAGGAGATGA AGAACCTGCG GAGCAAAGCG CTCAGCTCCT ACCTGATCCT AGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317813.1
    CDS1..2337
    Translation

    Target ORF information:

    RefSeq Version XM_015462327.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 221 (CFAP221), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462327.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCGGCACA TCCAGCCTCT GATAAAGCAA TGTGTTCATG TTAATGCTTC TTTTTCTCTC 
    CAGCATCTGG TCAATATTTG TGATGAAGAC ATACATGTTC ATATTTTACC CCCGCAAACC
    AAATACTTTC AGATCAAATA TGTTAAAAAG GAGCACCGCC TTGTCCCTGG CTTGTCTCTC
    AGGATCACCA TTACATTTTC TCCAGATGAG TGGCGATACT ATTATGATTG CATCCGTGTT
    CACTGTAAGG GAGACGAGAC TTTGCTTATT CCCATTCATG CCTATCCTGT CGTGAACACA
    CTGGACTTTC CCTCATTTAT AAATCTATCA AATGTTCTAC TTGGTGAAAG CAAAAGCTAC
    GTGATTCCTT TGTGGTGCAG TTGCCCTGTG GATTTTGAGT TTCATGTCAC TGTGGTTCAT
    CCTCATCAGG CCTTTACTGT GGAGCCAACA TCAGGAATAA TCCCGGCGAA TGGGAAGATG
    AATGTGACAG TGAAGTTTAC CCCGTTTCAG TATGGGATGG CACAGATAAA AGTGCAGCTG
    TGGGTTTCAC AGTTCAACTC TCAACCGTAT GAGTGTGTCT TCACCGGGAC ATGCTACCCC
    AATGTGGCTT TAAAGTTGGA AGAATTTGAA AGAGTAAATA ATCTTTCTAA AAAAGTAGTT
    GTTCCTCCAG AAAAGGCAAT GACACGTGTA CCTTTTCCCC AATTGCCAGC AAAAGCAAAG
    CCTCCAAAGA TTAAGGAAAT CGAATATCAC AACTTGAGAT TTCCAGTAGA TTTATTGAGT
    CCATTTGCTG TGGCAACTGT TTTAAACCAA GAACCAGGAA AATTAAAGAT TAAAGAATTG
    AGAGAAGTTT TGGACCAAGG CACTGAGATT TCAAAAACAA GACAAATGAA AGAAGCGCTC
    TTTGAACAGA AAGTCAGACA GGACACTTGT GAAGAGATTG AAAATCACCT GAAGTGGCAG
    GTGCACCTCG GTAAGGATCA TATGTCCTTT AAATTTAAAA AAGACATTTA TGAGGAGCGG
    CAAAGAGTAA ACAACAAATA CAAGCTAGAT AGAGGAGATC CTATTTTGTA TGAGGAATTC
    CAGCGACGCA AGACAGAAGT TCACCCCAAA CGGATTTTAC ACGACCTGAA AGAAAAAATC
    CAAGAATTTC ATCCTAATTT TGATCCACTC ATTAATAATA CTTGGGTCAA CAGATTCCGA
    GCACAGAGAC GGTTTCAACA AGCGGCTCGC AAGGTCCTGA TTCATCGCCG CTTGCTTACT
    ATGCTGAATG CCGTCCGTAA CATACAGAAA GACATCATTA TGAAAAGGTT CACTCAGGAA
    GGACAGTCCA TGGCACAAGA CAAGAGCTTC AAATACCACC TGGGGCGAGT CAGTAAGGAC
    GCCAGCCGGT TCTCTGTGTC TCCCCAGGAA GTACTGCCCT TTGCCTTTCC GTCCTACAGC
    CCTCCCCAGG ACTCCAACGA ACTGGCTCCT GGTGGCCTTG GACCTGTCCC AATTAAATCT
    TCAGAAATTC AAGTCAAGCA GCCCCATTCC TTCTTCAATC TGCAGGTTCC TCAGCTATAC
    AAGATCAAGA GGTACCAGCC AGTCTCTGTG CAGGAGTCTT CAGCCAGCTA CAGACCTCAG
    AAGCTTGCTC GGGCCCTGAG GCAAGGAGCT GAGGACGAAG CCACCACGGT CATCACCCTG
    CCCAAGCAGG ATTCCTCGCC CCAGCTGCCC GGTGAGACCT CCGTCCTGAG CATAAAACTC
    CCCGAGGCCT TGGCCCACGC TCCAGATTAC GACCCGCTTT ATATTTTTAA TCCCAGCCCG
    GGATTGTTTG CTGTGAAGCA CCCTCTGACT TACGTGGAAA CCTTGATCGA TTACCACCTG
    TGCTCCCACC CCAAGTACAA GTTCACCAAG GAGTGCCACT GCGGCTCCAG CATCCCCATC
    ACCCAGAAGC AATTTCTCCA TCACACGGAC ATAATACCTG GAGTGATGAC CTGGAAGAGG
    TTCCAGAGCC TGGTGTTCTT ATCCCTGCCC GAAACTTCTA AGACAGAGGT GTTGCCGAGC
    TGTGATTCCT TCAACTTAGT CATGCTGCCT GTCGACGTCC CTGCCGTTCT TGACGCCTTG
    CCGGAAGAAG ACAGGATAGA GACAGTTGAA CGTGAGCTCT GTGAACAGAG TATGGACATT
    ATGCTGACTC CAGAAATGAT CGAAGCGGAA TTCCCAATGC TGGATTACAG GGACACCAAG
    AAAGAGAAAG AGGTGAAAGA CCAAGCACAG CCAACGGAGA AGGCAGGAGA GAAGGTGCTC
    GAGGAGATGA AGAACCTGCG GAGCAAAGCG CTCAGCTCCT ACCTGATCCT AGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.