SIRT1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SIRT1
Entrez Gene ID	613629
Full Name	sirtuin 1
General protein information	Preferred Names sirtuin 1 Names NAD-dependent protein deacetylase sirtuin-1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024986766.1	XP_024842534.1	NAD-dependent protein deacetylase sirtuin-1 isoform X1
NM_001192980.3	NP_001179909.3	NAD-dependent protein deacetylase sirtuin-1
XM_015461011.1	XP_015316497.1	NAD-dependent protein deacetylase sirtuin-1 isoform X1
NM_001192980.1	NP_001179909.1	NAD-dependent protein deacetylase sirtuin-1
NM_001192980.2	NP_001179909.2	NAD-dependent protein deacetylase sirtuin-1
XM_024986767.1	XP_024842535.1	NAD-dependent protein deacetylase sirtuin-1 isoform X2
XM_015461011.2	XP_015316497.1	NAD-dependent protein deacetylase sirtuin-1 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SIRT1	XP_003312628.1
Rattus norvegicus (Norway rat)	Sirt1	XP_006256208.1
Xenopus tropicalis (tropical clawed frog)	sirt1	NP_001136381.1
Homo sapiens (human)	SIRT1	NP_036370.2
Canis lupus familiaris (dog)	SIRT1	XP_546130.2
Gallus gallus (chicken)	SIRT1	NP_001004767.1
	SIRT1	XP_001087854.2
Danio rerio (zebrafish)	sirt1	XP_005173091.1
Bos taurus (cattle)	SIRT1	NP_001179909.1
Mus musculus (house mouse)	Sirt1	NP_062786.1

Gene Ontology
Bibliography

Related articles in PubMed

Resveratrol induces apoptosis and inhibits adipogenesis by stimulating the SIRT1-AMPK?-FOXO1 signalling pathway in bovine intramuscular adipocytes.
Liu X, Zhao H, Jin Q, You W, Cheng H, Liu Y, Song E, Liu G, Tan X, Zhang X, Wan F
Molecular and cellular biochemistry(2017 Aug)

Haplotype distribution in the class I sirtuin genes and their associations with ultrasound carcass traits in Qinchuan cattle (Bos taurus).
Gui L, Hao R, Zhang Y, Zhao X, Zan L
Molecular and cellular probes29(3)167-71(2015 Jun)

Molecular characterization, expression profiles, and analysis of Qinchuan cattle SIRT1 gene association with meat quality and body measurement traits (Bos taurus).
Gui L, Wang H, Wei S, Zhang Y, Zan L
Molecular biology reports41(8)5237-46(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results indicate that the variations in the class I sirtuin genes and their corresponding genotypes may be considered as molecular markers for economic traits in cattle breeding.
Title: Haplotype distribution in the class I sirtuin genes and their associations with ultrasound carcass traits in Qinchuan cattle (Bos taurus).

These results suggest that the SIRT1 gene could be used in marker assisted selection to improve the production traits of Qinchuan cattle.
Title: Molecular characterization, expression profiles, and analysis of Qinchuan cattle SIRT1 gene association with meat quality and body measurement traits (Bos taurus).

Downregulation of SIRT1 and FoxO1 were observed in the backfat tissue of Lilu cattle with increasing age. SIRT1 may play an important role in the development of bovine adipose tissue.
Title: Gene expression profiling of SIRT1, FoxO1, and PPAR? in backfat tissues and subcutaneous adipocytes of Lilu bulls.

5 novel SNPs were found in SIRT1. SNP g.-274C>G was shown to have association with 24-months-old body weight and g.17379A>G polymorphism was significantly related to 6 and 12-months-old body weight in NY population; results provide evidence that polymorphisms in SIRT1 are associated with growth efficiency traits
Title: SIRT1 gene polymorphisms are associated with growth traits in Nanyang cattle.

Study indicates that a variation in the SIRT1 gene, the c.-274G variant in the promoter region, is associated with greater body size in Nanyang cattle.
Title: A novel c.-274C>G polymorphism in bovine SIRT1 gene contributes to diminished promoter activity and is associated with increased body size.

The following SIRT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIRT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa231668	XM_015461011.2 Latest version!	Bos taurus sirtuin 1 (SIRT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa88927	XM_015461011.1 Latest version!	Bos taurus sirtuin 1 (SIRT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBa239219	NM_001192980.3 Latest version!	Bos taurus sirtuin 1 (SIRT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa231666	XM_024986766.1 Latest version!	Bos taurus sirtuin 1 (SIRT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa231667	XM_024986767.1 Latest version!	Bos taurus sirtuin 1 (SIRT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa01331	NM_001192980.1 Latest version!	Bos taurus sirtuin 1 (SIRT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa216304	NM_001192980.2 Latest version!	Bos taurus sirtuin 1 (SIRT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIRT1 ( XM_015461011.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316497.1 Bos taurus sirtuin 1 (SIRT1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa231668
Clone ID Related Accession (Same CDS sequence)	XM_015461011.2
Accession Version	XM_015461011.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NAD-dependent protein deacetylase sirtuin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCTGT GCAGTGGAAG GAAAACAATT TTGGAAATAT ATCCTGGACA ATTCCAACCA 
TCTCTTTGTC ACAAATTCAT AGCCTTGTCT GATAAGGAAG GAAAACTACT TCGCAACTAT 
ACTCAGAACA TAGATACACT GGAGCAGGTT GCAGGAATCC AAAAGATAAT TCAGTGTCAT 
GGTTCCTTTG CAACAGCATC TTGCCTGATT TGTAAATATA AAGTTGACTG TGAAGCTGTA 
CGAGGAGATA TTTTTAATCA GGTGGTTCCT CGATGTCCTA GATGCCCAGC TGATGAACCG 
CTTGCTATCA TGAAACCAGA GATTGTCTTT TTTGGTGAAA ATTTACCAGA ACAGTTTCAT 
AGAGCCATGA AGTATGACAA AGATGAAGTT GATCTTCTCA TTGTTATTGG ATCTTCCCTG 
AAAGTAAGAC CAGTAGCACT AATTCCAAGT TCCATACCCC ATGAAGTGCC TCAGATATTA 
ATTAATAGGG AACCTTTGCC TCACCTGCAT TTTGATGTAG AGCTTCTTGG AGACTGTGAC 
GTAATTATTA ATGAACTGTG TCATAGGTTA GGTGGTGAAT ATGCCAAACT TTGCTGTAAC 
CCTGTGAAGC TTTCAGAAAT TACTGAAAAA CCTCCTCGAA TACAAAAAGA GTTGGCACAT 
TTGTCAGAAT TGCCACCCAC ACCCCTCAAT ATTTCAGAAG GCTCAAGTTC ACCAGAAAGA 
ACTTCACCAC CAGATTCTTC AGTGATTGTC ACTCTTTTAG ACCAAGAAAC AAAGAGTAAC 
GTTGATGATC CAGATGTGTC TGAATCAAAA GACCATGTCA CAGAAAAATC ACAGGAAGTA 
CAGACTTCTA CTAGGAGCAT TGAAAGTGTT AATGAACAGT TGGAGAGTCC AGATTTGAAG 
AATGCTGTCT CCAATTCTGG TGAGAAAAAT GAAAGAACTT CAGTAGCTGA AACAGTGAGA 
AAATGCTGGC CAGCTAGACT TGCAAAGGAG CAGATTAGTA AACGCCTTGA TGATAATCAG 
TATCTGTTTT TACCACCAAA CCGTTACATT TTCCATGGCG CTGAGGTATA TTCAGACTCT 
GAAGATGATG TCTTATCCTC TAGTTCTTGC GGCAGTAACA GTGATAGTGG AACGTGCCAG 
AGTCCAAGTT TAGAAGAACC CATGGAGGAT GAAAGTGAGA ATGAAGAATT TTACAATGGT 
TTGGAAGATG ATGCTGATGT TAATGAGAGA GCTGGAGGAA CTGTATTTGA AGCTGATGGA 
GGTGATCAAG AGGCAATTAA TGAAGCTATA TCTGTGAAAC AGGAAGCAAC ATGCATTAAC 
TATCCATCAA ACAAATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316497.1
CDS	233..1573
Translation	MCLCSGRKTILEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQKIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRIQKELAHLSELPPTPLNISEGSSSPERTSPPDSSVIVTLLDQETKSNVDDPDVSESKDHVTEKSQEVQTSTRSIESVNEQLESPDLKNAVSNSGEKNERTSVAETVRKCWPARLAKEQISKRLDDNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESENEEFYNGLEDDADVNERAGGTVFEADGGDQEAINEAISVKQEATCINYPSNKS

Target ORF information:

RefSeq Version	XM_015461011.2
Organism	Bos taurus(cattle)
Definition	Bos taurus sirtuin 1 (SIRT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461011.2

ORF Insert Sequence:

ATGTGCCTGT GCAGTGGAAG GAAAACAATT TTGGAAATAT ATCCTGGACA ATTCCAACCA 
TCTCTTTGTC ACAAATTCAT AGCCTTGTCT GATAAGGAAG GAAAACTACT TCGCAACTAT 
ACTCAGAACA TAGATACACT GGAGCAGGTT GCAGGAATCC AAAAGATAAT TCAGTGTCAT 
GGTTCCTTTG CAACAGCATC TTGCCTGATT TGTAAATATA AAGTTGACTG TGAAGCTGTA 
CGAGGAGATA TTTTTAATCA GGTGGTTCCT CGATGTCCTA GATGCCCAGC TGATGAACCG 
CTTGCTATCA TGAAACCAGA GATTGTCTTT TTTGGTGAAA ATTTACCAGA ACAGTTTCAT 
AGAGCCATGA AGTATGACAA AGATGAAGTT GATCTTCTCA TTGTTATTGG ATCTTCCCTG 
AAAGTAAGAC CAGTAGCACT AATTCCAAGT TCCATACCCC ATGAAGTGCC TCAGATATTA 
ATTAATAGGG AACCTTTGCC TCACCTGCAT TTTGATGTAG AGCTTCTTGG AGACTGTGAC 
GTAATTATTA ATGAACTGTG TCATAGGTTA GGTGGTGAAT ATGCCAAACT TTGCTGTAAC 
CCTGTGAAGC TTTCAGAAAT TACTGAAAAA CCTCCTCGAA TACAAAAAGA GTTGGCACAT 
TTGTCAGAAT TGCCACCCAC ACCCCTCAAT ATTTCAGAAG GCTCAAGTTC ACCAGAAAGA 
ACTTCACCAC CAGATTCTTC AGTGATTGTC ACTCTTTTAG ACCAAGAAAC AAAGAGTAAC 
GTTGATGATC CAGATGTGTC TGAATCAAAA GACCATGTCA CAGAAAAATC ACAGGAAGTA 
CAGACTTCTA CTAGGAGCAT TGAAAGTGTT AATGAACAGT TGGAGAGTCC AGATTTGAAG 
AATGCTGTCT CCAATTCTGG TGAGAAAAAT GAAAGAACTT CAGTAGCTGA AACAGTGAGA 
AAATGCTGGC CAGCTAGACT TGCAAAGGAG CAGATTAGTA AACGCCTTGA TGATAATCAG 
TATCTGTTTT TACCACCAAA CCGTTACATT TTCCATGGCG CTGAGGTATA TTCAGACTCT 
GAAGATGATG TCTTATCCTC TAGTTCTTGC GGCAGTAACA GTGATAGTGG AACGTGCCAG 
AGTCCAAGTT TAGAAGAACC CATGGAGGAT GAAAGTGAGA ATGAAGAATT TTACAATGGT 
TTGGAAGATG ATGCTGATGT TAATGAGAGA GCTGGAGGAA CTGTATTTGA AGCTGATGGA 
GGTGATCAAG AGGCAATTAA TGAAGCTATA TCTGTGAAAC AGGAAGCAAC ATGCATTAAC 
TATCCATCAA ACAAATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT1 ( XM_015461011.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316497.1 Bos taurus sirtuin 1 (SIRT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa88927
Clone ID Related Accession (Same CDS sequence)	XM_015461011.1
Accession Version	XM_015461011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NAD-dependent protein deacetylase sirtuin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCTGT GCAGTGGAAG GAAAACAATT TTGGAAATAT ATCCTGGACA ATTCCAACCA 
TCTCTTTGTC ACAAATTCAT AGCCTTGTCT GATAAGGAAG GAAAACTACT TCGCAACTAT 
ACTCAGAACA TAGATACACT GGAGCAGGTT GCAGGAATCC AAAAGATAAT TCAGTGTCAT 
GGTTCCTTTG CAACAGCATC TTGCCTGATT TGTAAATATA AAGTTGACTG TGAAGCTGTA 
CGAGGAGATA TTTTTAATCA GGTGGTTCCT CGATGTCCTA GATGCCCAGC TGATGAACCG 
CTTGCTATCA TGAAACCAGA AATTGTCTTT TTTGGTGAAA ATTTACCAGA ACAGTTTCAT 
AGAGCCATGA AGTATGACAA AGATGAAGTT GATCTTCTCA TTGTTATTGG ATCTTCCCTG 
AAAGTAAGAC CAGTAGCACT AATTCCAAGT TCCATACCCC ATGAAGTGCC TCAGATATTA 
ATTAATAGGG AACCTTTGCC TCACCTGCAT TTTGATGTAG AGCTTCTTGG AGACTGTGAC 
GTAATTATTA ATGAACTGTG TCATAGGTTA GGTGGTGAAT ATGCCAAACT TTGCTGTAAC 
CCTGTGAAGC TTTCAGAAAT TACTGAAAAA CCTCCTCGAA TACAAAAAGA GTTGGCACAT 
TTGTCAGAAT TGCCACCCAC ACCCCTCAAT ATTTCAGAAG GCTCAAGTTC ACCAGAAAGA 
ACTTCACCAC CAGATTCTTC AGTGATTGTC ACTCTTTTAG ACCAAGAAAC AAAGAGTAAC 
GTTGATGATC CAGATGTGTC TGAATCAAAA GACCATGTCA CAGAAAAATC ACAGGAAGTA 
CAGACTTCTA CTAGGAGCAT TGAAAGTGTT AATGAACAGT TGGAGAGTCC AGATTTGAAG 
AATGCTGTCT CCAATTCTGG TGAGAAAAAT GAAAGAACTT CAGTAGCTGA AACAGTGAGA 
AAATGCTGGC CAGCTAGACT TGCAAAGGAG CAGATTAGTA AACGCCTTGA TGATAATCAG 
TATCTGTTTT TACCACCAAA CCGTTACATT TTCCATGGCG CTGAGGTATA TTCAGACTCT 
GAAGATGATG TCTTATCCTC TAGTTCTTGC GGCAGTAACA GTGATAGTGG AACGTGCCAG 
AGTCCAAGTT TAGAAGAACC CATGGAGGAT GAAAGTGAGA ATGAAGAATT TTACAATGGT 
TTGGAAGATG ATGCTGATGT TAATGAGAGA GCTGGAGGAA CTGTATTTGA AGCTGATGGA 
GGTGATCAAG AGGCAATTAA TGAAGCTATA TCTGTGAAAC AGGAAGCAAC ATGCATTAAC 
TATCCATCAA ACAAATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316497.1
CDS	91..1431
Translation	MCLCSGRKTILEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQKIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRIQKELAHLSELPPTPLNISEGSSSPERTSPPDSSVIVTLLDQETKSNVDDPDVSESKDHVTEKSQEVQTSTRSIESVNEQLESPDLKNAVSNSGEKNERTSVAETVRKCWPARLAKEQISKRLDDNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESENEEFYNGLEDDADVNERAGGTVFEADGGDQEAINEAISVKQEATCINYPSNKS

Target ORF information:

RefSeq Version	XM_015461011.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sirtuin 1 (SIRT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461011.1

ORF Insert Sequence:

ATGTGCCTGT GCAGTGGAAG GAAAACAATT TTGGAAATAT ATCCTGGACA ATTCCAACCA 
TCTCTTTGTC ACAAATTCAT AGCCTTGTCT GATAAGGAAG GAAAACTACT TCGCAACTAT 
ACTCAGAACA TAGATACACT GGAGCAGGTT GCAGGAATCC AAAAGATAAT TCAGTGTCAT 
GGTTCCTTTG CAACAGCATC TTGCCTGATT TGTAAATATA AAGTTGACTG TGAAGCTGTA 
CGAGGAGATA TTTTTAATCA GGTGGTTCCT CGATGTCCTA GATGCCCAGC TGATGAACCG 
CTTGCTATCA TGAAACCAGA AATTGTCTTT TTTGGTGAAA ATTTACCAGA ACAGTTTCAT 
AGAGCCATGA AGTATGACAA AGATGAAGTT GATCTTCTCA TTGTTATTGG ATCTTCCCTG 
AAAGTAAGAC CAGTAGCACT AATTCCAAGT TCCATACCCC ATGAAGTGCC TCAGATATTA 
ATTAATAGGG AACCTTTGCC TCACCTGCAT TTTGATGTAG AGCTTCTTGG AGACTGTGAC 
GTAATTATTA ATGAACTGTG TCATAGGTTA GGTGGTGAAT ATGCCAAACT TTGCTGTAAC 
CCTGTGAAGC TTTCAGAAAT TACTGAAAAA CCTCCTCGAA TACAAAAAGA GTTGGCACAT 
TTGTCAGAAT TGCCACCCAC ACCCCTCAAT ATTTCAGAAG GCTCAAGTTC ACCAGAAAGA 
ACTTCACCAC CAGATTCTTC AGTGATTGTC ACTCTTTTAG ACCAAGAAAC AAAGAGTAAC 
GTTGATGATC CAGATGTGTC TGAATCAAAA GACCATGTCA CAGAAAAATC ACAGGAAGTA 
CAGACTTCTA CTAGGAGCAT TGAAAGTGTT AATGAACAGT TGGAGAGTCC AGATTTGAAG 
AATGCTGTCT CCAATTCTGG TGAGAAAAAT GAAAGAACTT CAGTAGCTGA AACAGTGAGA 
AAATGCTGGC CAGCTAGACT TGCAAAGGAG CAGATTAGTA AACGCCTTGA TGATAATCAG 
TATCTGTTTT TACCACCAAA CCGTTACATT TTCCATGGCG CTGAGGTATA TTCAGACTCT 
GAAGATGATG TCTTATCCTC TAGTTCTTGC GGCAGTAACA GTGATAGTGG AACGTGCCAG 
AGTCCAAGTT TAGAAGAACC CATGGAGGAT GAAAGTGAGA ATGAAGAATT TTACAATGGT 
TTGGAAGATG ATGCTGATGT TAATGAGAGA GCTGGAGGAA CTGTATTTGA AGCTGATGGA 
GGTGATCAAG AGGCAATTAA TGAAGCTATA TCTGTGAAAC AGGAAGCAAC ATGCATTAAC 
TATCCATCAA ACAAATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT1 ( NM_001192980.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179909.3 Bos taurus sirtuin 1 (SIRT1), mRNA.

Price & Availability↑

CloneID	OBa239219
Clone ID Related Accession (Same CDS sequence)	NM_001192980.3
Accession Version	NM_001192980.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Bos taurus(cattle)
Product	NAD-dependent protein deacetylase sirtuin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000028.1. On May 29, 2018 this sequence version replaced NM_001192980.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACG AGGCGGCGCT CGCCCTTCAG CCCGGCGGCT CTCCCTCGGT AGTGGCGGCT 
GAGAGGGAGG CCCCGTCGCC GCCTGCTGGG GAGCCGCTCC GCAAGAGGCC GCGAAGAGAC 
GGCCCCGGCG TCGGGCGGAG CTCGGGCGAG CCCGGTGGGA CGGCCCCCGA GCGGGAACTG 
CCGGCGGCGG CCGGCAGTTG CCCGGCGGCG GCGGCGGCGC TGTGGCGGGA GGCCCAGGCG 
GCGGCGGCGG CGGCGGCCGA AGAAGAAGAC AATGGGCCGG GCTTACAGGG CCTATCCAGG 
GAGGCGCCAC CGGCCGACGA CTTCTACGAC GACGACGACG AGGGCGAGGA GGAGGAAGAG 
GCGGCGGCCG CTATTGGGTA CCGAGATAAC CTTCTGTTTG GTGATGAAAT CATCACCAAC 
GGTTTCCATT CGTGTGAAAG TGACGAAGAT GATAGAGCCT CGCATGCGAG TTCTAGTGAC 
TGGACTCCAA GACCTCGGAT AGGTCCATAT ACTTTTGTTC AGCAACATCT CATGATTGGC 
ACAGATCCTC GAACAATTCT TAAAGATTTA CTACCAGAAA CAATTCCTCC ACCTGAATTG 
GATGATATGA CACTGTGGCA GATTGTTATT AATATCCTTT CAGAACCACC AAAAAGGAAA 
AAAAGAAAAG ATATTAATAC AATTGAAGAT GCTGTGAAAT TACTGCAAGA GTGCAAAAAA 
ATAATAGTTC TAACTGGAGC TGGGGTTTCT GTTTCTTGTG GAATACCTGA CTTCAGGTCA 
AGAGACGGTA TTTATGCTCG CCTTGCAATA GACTTTCCAG ACCTTCCAGA TCCTCAAGCA 
ATGTTTGATA TTGAATATTT CAGAAAAGAC CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
ATATATCCTG GACAATTCCA ACCATCTCTT TGTCACAAAT TCATAGCCTT GTCTGATAAG 
GAAGGAAAAC TACTTCGCAA CTATACTCAG AACATAGATA CACTGGAGCA GGTTGCAGGA 
ATCCAAAAGA TAATTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA 
TATAAAGTTG ACTGTGAAGC TGTACGAGGA GATATTTTTA ATCAGGTGGT TCCTCGATGT 
CCTAGATGCC CAGCTGATGA ACCGCTTGCT ATCATGAAAC CAGAGATTGT CTTTTTTGGT 
GAAAATTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGATCTT 
CTCATTGTTA TTGGATCTTC CCTGAAAGTA AGACCAGTAG CACTAATTCC AAGTTCCATA 
CCCCATGAAG TGCCTCAGAT ATTAATTAAT AGGGAACCTT TGCCTCACCT GCATTTTGAT 
GTAGAGCTTC TTGGAGACTG TGACGTAATT ATTAATGAAC TGTGTCATAG GTTAGGTGGT 
GAATATGCCA AACTTTGCTG TAACCCTGTG AAGCTTTCAG AAATTACTGA AAAACCTCCT 
CGAATACAAA AAGAGTTGGC ACATTTGTCA GAATTGCCAC CCACACCCCT CAATATTTCA 
GAAGGCTCAA GTTCACCAGA AAGAACTTCA CCACCAGATT CTTCAGTGAT TGTCACTCTT 
TTAGACCAAG AAACAAAGAG TAACGTTGAT GATCCAGATG TGTCTGAATC AAAAGACCAT 
GTCACAGAAA AATCACAGGA AGTACAGACT TCTACTAGGA GCATTGAAAG TGTTAATGAA 
CAGTTGGAGA GTCCAGATTT GAAGAATGCT GTCTCCAATT CTGGTGAGAA AAATGAAAGA 
ACTTCAGTAG CTGAAACAGT GAGAAAATGC TGGCCAGCTA GACTTGCAAA GGAGCAGATT 
AGTAAACGCC TTGATGATAA TCAGTATCTG TTTTTACCAC CAAACCGTTA CATTTTCCAT 
GGCGCTGAGG TATATTCAGA CTCTGAAGAT GATGTCTTAT CCTCTAGTTC TTGCGGCAGT 
AACAGTGATA GTGGAACGTG CCAGAGTCCA AGTTTAGAAG AACCCATGGA GGATGAAAGT 
GAGAATGAAG AATTTTACAA TGGTTTGGAA GATGATGCTG ATGTTAATGA GAGAGCTGGA 
GGAACTGTAT TTGAAGCTGA TGGAGGTGAT CAAGAGGCAA TTAATGAAGC TATATCTGTG 
AAACAGGAAG CAACATGCAT TAACTATCCA TCAAACAAAT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179909.3
CDS	34..2238
Translation	MADEAALALQPGGSPSVVAAEREAPSPPAGEPLRKRPRRDGPGVGRSSGEPGGTAPERELPAAAGSCPAAAAALWREAQAAAAAAAEEEDNGPGLQGLSREAPPADDFYDDDDEGEEEEEAAAAIGYRDNLLFGDEIITNGFHSCESDEDDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAIDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQKIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRIQKELAHLSELPPTPLNISEGSSSPERTSPPDSSVIVTLLDQETKSNVDDPDVSESKDHVTEKSQEVQTSTRSIESVNEQLESPDLKNAVSNSGEKNERTSVAETVRKCWPARLAKEQISKRLDDNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESENEEFYNGLEDDADVNERAGGTVFEADGGDQEAINEAISVKQEATCINYPSNKS

Target ORF information:

RefSeq Version	NM_001192980.3
Organism	Bos taurus(cattle)
Definition	Bos taurus sirtuin 1 (SIRT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192980.3

ORF Insert Sequence:

ATGGCGGACG AGGCGGCGCT CGCCCTTCAG CCCGGCGGCT CTCCCTCGGT AGTGGCGGCT 
GAGAGGGAGG CCCCGTCGCC GCCTGCTGGG GAGCCGCTCC GCAAGAGGCC GCGAAGAGAC 
GGCCCCGGCG TCGGGCGGAG CTCGGGCGAG CCCGGTGGGA CGGCCCCCGA GCGGGAACTG 
CCGGCGGCGG CCGGCAGTTG CCCGGCGGCG GCGGCGGCGC TGTGGCGGGA GGCCCAGGCG 
GCGGCGGCGG CGGCGGCCGA AGAAGAAGAC AATGGGCCGG GCTTACAGGG CCTATCCAGG 
GAGGCGCCAC CGGCCGACGA CTTCTACGAC GACGACGACG AGGGCGAGGA GGAGGAAGAG 
GCGGCGGCCG CTATTGGGTA CCGAGATAAC CTTCTGTTTG GTGATGAAAT CATCACCAAC 
GGTTTCCATT CGTGTGAAAG TGACGAAGAT GATAGAGCCT CGCATGCGAG TTCTAGTGAC 
TGGACTCCAA GACCTCGGAT AGGTCCATAT ACTTTTGTTC AGCAACATCT CATGATTGGC 
ACAGATCCTC GAACAATTCT TAAAGATTTA CTACCAGAAA CAATTCCTCC ACCTGAATTG 
GATGATATGA CACTGTGGCA GATTGTTATT AATATCCTTT CAGAACCACC AAAAAGGAAA 
AAAAGAAAAG ATATTAATAC AATTGAAGAT GCTGTGAAAT TACTGCAAGA GTGCAAAAAA 
ATAATAGTTC TAACTGGAGC TGGGGTTTCT GTTTCTTGTG GAATACCTGA CTTCAGGTCA 
AGAGACGGTA TTTATGCTCG CCTTGCAATA GACTTTCCAG ACCTTCCAGA TCCTCAAGCA 
ATGTTTGATA TTGAATATTT CAGAAAAGAC CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
ATATATCCTG GACAATTCCA ACCATCTCTT TGTCACAAAT TCATAGCCTT GTCTGATAAG 
GAAGGAAAAC TACTTCGCAA CTATACTCAG AACATAGATA CACTGGAGCA GGTTGCAGGA 
ATCCAAAAGA TAATTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA 
TATAAAGTTG ACTGTGAAGC TGTACGAGGA GATATTTTTA ATCAGGTGGT TCCTCGATGT 
CCTAGATGCC CAGCTGATGA ACCGCTTGCT ATCATGAAAC CAGAGATTGT CTTTTTTGGT 
GAAAATTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGATCTT 
CTCATTGTTA TTGGATCTTC CCTGAAAGTA AGACCAGTAG CACTAATTCC AAGTTCCATA 
CCCCATGAAG TGCCTCAGAT ATTAATTAAT AGGGAACCTT TGCCTCACCT GCATTTTGAT 
GTAGAGCTTC TTGGAGACTG TGACGTAATT ATTAATGAAC TGTGTCATAG GTTAGGTGGT 
GAATATGCCA AACTTTGCTG TAACCCTGTG AAGCTTTCAG AAATTACTGA AAAACCTCCT 
CGAATACAAA AAGAGTTGGC ACATTTGTCA GAATTGCCAC CCACACCCCT CAATATTTCA 
GAAGGCTCAA GTTCACCAGA AAGAACTTCA CCACCAGATT CTTCAGTGAT TGTCACTCTT 
TTAGACCAAG AAACAAAGAG TAACGTTGAT GATCCAGATG TGTCTGAATC AAAAGACCAT 
GTCACAGAAA AATCACAGGA AGTACAGACT TCTACTAGGA GCATTGAAAG TGTTAATGAA 
CAGTTGGAGA GTCCAGATTT GAAGAATGCT GTCTCCAATT CTGGTGAGAA AAATGAAAGA 
ACTTCAGTAG CTGAAACAGT GAGAAAATGC TGGCCAGCTA GACTTGCAAA GGAGCAGATT 
AGTAAACGCC TTGATGATAA TCAGTATCTG TTTTTACCAC CAAACCGTTA CATTTTCCAT 
GGCGCTGAGG TATATTCAGA CTCTGAAGAT GATGTCTTAT CCTCTAGTTC TTGCGGCAGT 
AACAGTGATA GTGGAACGTG CCAGAGTCCA AGTTTAGAAG AACCCATGGA GGATGAAAGT 
GAGAATGAAG AATTTTACAA TGGTTTGGAA GATGATGCTG ATGTTAATGA GAGAGCTGGA 
GGAACTGTAT TTGAAGCTGA TGGAGGTGAT CAAGAGGCAA TTAATGAAGC TATATCTGTG 
AAACAGGAAG CAACATGCAT TAACTATCCA TCAAACAAAT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT1 ( XM_024986766.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842534.1 Bos taurus sirtuin 1 (SIRT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa231666
Clone ID Related Accession (Same CDS sequence)	XM_024986766.1
Accession Version	XM_024986766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NAD-dependent protein deacetylase sirtuin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG AGGCGGCGCT CGCCCTTCAG CCCGGCGGCT CTCCCTCGGT AGTGGCGGCT 
GAGAGGGAGG CCCCGTCGCC GCCTGCTGGG GAGCCGCTCC GCAAGAGGCC GCGAAGAGAC 
GGCCCCGGCG TCGGGCGGAG CTCGGGCGAG CCCGGTGGGA CGGCCCCCGA GCGGGAACTG 
CCGGCGGCGG CCGGCAGTTG CCCGGCGGCG GCGGCGGCGC TGTGGCGGGA GGCCCAGGCG 
GCGGCGGCGG CGGCGGCCGA AGAAGAAGAC AATGGGCCGG GCTTACAGGG CCTATCCAGG 
GAGGCGCCAC CGGCCGACGA CTTCTACGAC GACGACGACG AGGGCGAGGA GGAGGAAGAG 
GCGGCGGCCG CTATTGGGTA CCGAGATAAC CTTCTGTTTG GTGATGAAAT CATCACCAAC 
GGTTTCCATT CGTGTGAAAG TGACGAAGAT GATAGAGCCT CGCATGCGAG TTCTAGTGAC 
TGGACTCCAA GACCTCGGAT AGGTCCATAT ACTTTTGTTC AGCAACATCT CATGATTGGC 
ACAGATCCTC GAACAATTCT TAAAGATTTA CTACCAGAAA CAATTCCTCC ACCTGAATTG 
GATGATATGA CACTGTGGCA GATTGTTATT AATATCCTTT CAGAACCACC AAAAAGGAAA 
AAAAGAAAAG ATATTAATAC AATTGAAGAT GCTGTGAAAT TACTGCAAGA GTGCAAAAAA 
ATAATAGTTC TAACTGGAGC TGGGGTTTCT GTTTCTTGTG GAATACCTGA CTTCAGGTCA 
AGAGACGGTA TTTATGCTCG CCTTGCAATA GACTTTCCAG ACCTTCCAGA TCCTCAAGCA 
ATGTTTGATA TTGAATATTT CAGAAAAGAC CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
ATATATCCTG GACAATTCCA ACCATCTCTT TGTCACAAAT TCATAGCCTT GTCTGATAAG 
GAAGGAAAAC TACTTCGCAA CTATACTCAG AACATAGATA CACTGGAGCA GGTTGCAGGA 
ATCCAAAAGA TAATTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA 
TATAAAGTTG ACTGTGAAGC TGTACGAGGA GATATTTTTA ATCAGGTGGT TCCTCGATGT 
CCTAGATGCC CAGCTGATGA ACCGCTTGCT ATCATGAAAC CAGAGATTGT CTTTTTTGGT 
GAAAATTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGATCTT 
CTCATTGTTA TTGGATCTTC CCTGAAAGTA AGACCAGTAG CACTAATTCC AAGTTCCATA 
CCCCATGAAG TGCCTCAGAT ATTAATTAAT AGGGAACCTT TGCCTCACCT GCATTTTGAT 
GTAGAGCTTC TTGGAGACTG TGACGTAATT ATTAATGAAC TGTGTCATAG GTTAGGTGGT 
GAATATGCCA AACTTTGCTG TAACCCTGTG AAGCTTTCAG AAATTACTGA AAAACCTCCT 
CGAATACAAA AAGAGTTGGC ACATTTGTCA GAATTGCCAC CCACACCCCT CAATATTTCA 
GAAGGCTCAA GTTCACCAGA AAGAACTTCA CCACCAGATT CTTCAGTGAT TGTCACTCTT 
TTAGACCAAG AAACAAAGAG TAACGTTGAT GATCCAGATG TGTCTGAATC AAAAGACCAT 
GTCACAGAAA AATCACAGGA AGTACAGACT TCTACTAGGA GCATTGAAAG TGTTAATGAA 
CAGTTGGAGA GTCCAGATTT GAAGAATGCT GTCTCCAATT CTGATAATCA GTATCTGTTT 
TTACCACCAA ACCGTTACAT TTTCCATGGC GCTGAGGTAT ATTCAGACTC TGAAGATGAT 
GTCTTATCCT CTAGTTCTTG CGGCAGTAAC AGTGATAGTG GAACGTGCCA GAGTCCAAGT 
TTAGAAGAAC CCATGGAGGA TGAAAGTGAG AATGAAGAAT TTTACAATGG TTTGGAAGAT 
GATGCTGATG TTAATGAGAG AGCTGGAGGA ACTGTATTTG AAGCTGATGG AGGTGATCAA 
GAGGCAATTA ATGAAGCTAT ATCTGTGAAA CAGGAAGCAA CATGCATTAA CTATCCATCA 
AACAAATCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842534.1
CDS	101..2212
Translation	MADEAALALQPGGSPSVVAAEREAPSPPAGEPLRKRPRRDGPGVGRSSGEPGGTAPERELPAAAGSCPAAAAALWREAQAAAAAAAEEEDNGPGLQGLSREAPPADDFYDDDDEGEEEEEAAAAIGYRDNLLFGDEIITNGFHSCESDEDDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAIDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQKIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRIQKELAHLSELPPTPLNISEGSSSPERTSPPDSSVIVTLLDQETKSNVDDPDVSESKDHVTEKSQEVQTSTRSIESVNEQLESPDLKNAVSNSDNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESENEEFYNGLEDDADVNERAGGTVFEADGGDQEAINEAISVKQEATCINYPSNKS

Target ORF information:

RefSeq Version	XM_024986766.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sirtuin 1 (SIRT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986766.1

ORF Insert Sequence:

ATGGCGGACG AGGCGGCGCT CGCCCTTCAG CCCGGCGGCT CTCCCTCGGT AGTGGCGGCT 
GAGAGGGAGG CCCCGTCGCC GCCTGCTGGG GAGCCGCTCC GCAAGAGGCC GCGAAGAGAC 
GGCCCCGGCG TCGGGCGGAG CTCGGGCGAG CCCGGTGGGA CGGCCCCCGA GCGGGAACTG 
CCGGCGGCGG CCGGCAGTTG CCCGGCGGCG GCGGCGGCGC TGTGGCGGGA GGCCCAGGCG 
GCGGCGGCGG CGGCGGCCGA AGAAGAAGAC AATGGGCCGG GCTTACAGGG CCTATCCAGG 
GAGGCGCCAC CGGCCGACGA CTTCTACGAC GACGACGACG AGGGCGAGGA GGAGGAAGAG 
GCGGCGGCCG CTATTGGGTA CCGAGATAAC CTTCTGTTTG GTGATGAAAT CATCACCAAC 
GGTTTCCATT CGTGTGAAAG TGACGAAGAT GATAGAGCCT CGCATGCGAG TTCTAGTGAC 
TGGACTCCAA GACCTCGGAT AGGTCCATAT ACTTTTGTTC AGCAACATCT CATGATTGGC 
ACAGATCCTC GAACAATTCT TAAAGATTTA CTACCAGAAA CAATTCCTCC ACCTGAATTG 
GATGATATGA CACTGTGGCA GATTGTTATT AATATCCTTT CAGAACCACC AAAAAGGAAA 
AAAAGAAAAG ATATTAATAC AATTGAAGAT GCTGTGAAAT TACTGCAAGA GTGCAAAAAA 
ATAATAGTTC TAACTGGAGC TGGGGTTTCT GTTTCTTGTG GAATACCTGA CTTCAGGTCA 
AGAGACGGTA TTTATGCTCG CCTTGCAATA GACTTTCCAG ACCTTCCAGA TCCTCAAGCA 
ATGTTTGATA TTGAATATTT CAGAAAAGAC CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
ATATATCCTG GACAATTCCA ACCATCTCTT TGTCACAAAT TCATAGCCTT GTCTGATAAG 
GAAGGAAAAC TACTTCGCAA CTATACTCAG AACATAGATA CACTGGAGCA GGTTGCAGGA 
ATCCAAAAGA TAATTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA 
TATAAAGTTG ACTGTGAAGC TGTACGAGGA GATATTTTTA ATCAGGTGGT TCCTCGATGT 
CCTAGATGCC CAGCTGATGA ACCGCTTGCT ATCATGAAAC CAGAGATTGT CTTTTTTGGT 
GAAAATTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGATCTT 
CTCATTGTTA TTGGATCTTC CCTGAAAGTA AGACCAGTAG CACTAATTCC AAGTTCCATA 
CCCCATGAAG TGCCTCAGAT ATTAATTAAT AGGGAACCTT TGCCTCACCT GCATTTTGAT 
GTAGAGCTTC TTGGAGACTG TGACGTAATT ATTAATGAAC TGTGTCATAG GTTAGGTGGT 
GAATATGCCA AACTTTGCTG TAACCCTGTG AAGCTTTCAG AAATTACTGA AAAACCTCCT 
CGAATACAAA AAGAGTTGGC ACATTTGTCA GAATTGCCAC CCACACCCCT CAATATTTCA 
GAAGGCTCAA GTTCACCAGA AAGAACTTCA CCACCAGATT CTTCAGTGAT TGTCACTCTT 
TTAGACCAAG AAACAAAGAG TAACGTTGAT GATCCAGATG TGTCTGAATC AAAAGACCAT 
GTCACAGAAA AATCACAGGA AGTACAGACT TCTACTAGGA GCATTGAAAG TGTTAATGAA 
CAGTTGGAGA GTCCAGATTT GAAGAATGCT GTCTCCAATT CTGATAATCA GTATCTGTTT 
TTACCACCAA ACCGTTACAT TTTCCATGGC GCTGAGGTAT ATTCAGACTC TGAAGATGAT 
GTCTTATCCT CTAGTTCTTG CGGCAGTAAC AGTGATAGTG GAACGTGCCA GAGTCCAAGT 
TTAGAAGAAC CCATGGAGGA TGAAAGTGAG AATGAAGAAT TTTACAATGG TTTGGAAGAT 
GATGCTGATG TTAATGAGAG AGCTGGAGGA ACTGTATTTG AAGCTGATGG AGGTGATCAA 
GAGGCAATTA ATGAAGCTAT ATCTGTGAAA CAGGAAGCAA CATGCATTAA CTATCCATCA 
AACAAATCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT1 ( XM_024986767.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842535.1 Bos taurus sirtuin 1 (SIRT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa231667
Clone ID Related Accession (Same CDS sequence)	XM_024986767.1
Accession Version	XM_024986767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NAD-dependent protein deacetylase sirtuin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG AGGCGGCGCT CGCCCTTCAG CCCGGCGGCT CTCCCTCGGT AGTGGCGGCT 
GAGAGGGAGG CCCCGTCGCC GCCTGCTGGG GAGCCGCTCC GCAAGAGGCC GCGAAGAGAC 
GGCCCCGGCG TCGGGCGGAG CTCGGGCGAG CCCGGTGGGA CGGCCCCCGA GCGGGAACTG 
CCGGCGGCGG CCGGCAGTTG CCCGGCGGCG GCGGCGGCGC TGTGGCGGGA GGCCCAGGCG 
GCGGCGGCGG CGGCGGCCGA AGAAGAAGAC AATGGGCCGG GCTTACAGGG CCTATCCAGG 
GAGGCGCCAC CGGCCGACGA CTTCTACGAC GACGACGACG AGGGCGAGGA GGAGGAAGAG 
GCGGCGGCCG CTATTGGGTA CCGAGATAAC CTTCTGTTTG GTGATGAAAT CATCACCAAC 
GGTTTCCATT CGTGTGAAAG TGACGAAGAT GATAGAGCCT CGCATGCGAG TTCTAGTGAC 
TGGACTCCAA GACCTCGGAT AGGTCCATAT ACTTTTGTTC AGCAACATCT CATGATTGGC 
ACAGATCCTC GAACAATTCT TAAAGATTTA CTACCAGAAA CAATTCCTCC ACCTGAATTG 
GATGATATGA CACTGTGGCA GATTGTTATT AATATCCTTT CAGAACCACC AAAAAGGAAA 
AAAAGAAAAG ATATTAATAC AATTGAAGAT GCTGTGAAAT TACTGCAAGA GTGCAAAAAA 
ATAATAGTTC TAACTGGAGC TGGGGTTTCT GTTTCTTGTG GAATACCTGA CTTCAGGTCA 
AGAGACGGTA TTTATGCTCG CCTTGCAATA GACTTTCCAG ACCTTCCAGA TCCTCAAGCA 
ATGTTTGATA TTGAATATTT CAGAAAAGAC CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
ATATATCCTG GACAATTCCA ACCATCTCTT TGTCACAAAT TCATAGCCTT GTCTGATAAG 
GAAGGAAAAC TACTTCGCAA CTATACTCAG AACATAGATA CACTGGAGCA GGTTGCAGGA 
ATCCAAAAGA TAATTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA 
TATAAAGTTG ACTGTGAAGC TGTACGAGGA GATATTTTTA ATCAGGTGGT TCCTCGATGT 
CCTAGATGCC CAGCTGATGA ACCGCTTGCT ATCATGAAAC CAGAGATTGT CTTTTTTGGT 
GAAAATTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGATCTT 
CTCATTGTTA TTGGATCTTC CCTGAAAGTA AGACCAGTAG CACTAATTCC AAATAATCAG 
TATCTGTTTT TACCACCAAA CCGTTACATT TTCCATGGCG CTGAGGTATA TTCAGACTCT 
GAAGATGATG TCTTATCCTC TAGTTCTTGC GGCAGTAACA GTGATAGTGG AACGTGCCAG 
AGTCCAAGTT TAGAAGAACC CATGGAGGAT GAAAGTGAGA ATGAAGAATT TTACAATGGT 
TTGGAAGATG ATGCTGATGT TAATGAGAGA GCTGGAGGAA CTGTATTTGA AGCTGATGGA 
GGTGATCAAG AGGCAATTAA TGAAGCTATA TCTGTGAAAC AGGAAGCAAC ATGCATTAAC 
TATCCATCAA ACAAATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842535.1
CDS	104..1744
Translation	MADEAALALQPGGSPSVVAAEREAPSPPAGEPLRKRPRRDGPGVGRSSGEPGGTAPERELPAAAGSCPAAAAALWREAQAAAAAAAEEEDNGPGLQGLSREAPPADDFYDDDDEGEEEEEAAAAIGYRDNLLFGDEIITNGFHSCESDEDDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAIDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQKIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPNNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESENEEFYNGLEDDADVNERAGGTVFEADGGDQEAINEAISVKQEATCINYPSNKS

Target ORF information:

RefSeq Version	XM_024986767.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sirtuin 1 (SIRT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986767.1

ORF Insert Sequence:

ATGGCGGACG AGGCGGCGCT CGCCCTTCAG CCCGGCGGCT CTCCCTCGGT AGTGGCGGCT 
GAGAGGGAGG CCCCGTCGCC GCCTGCTGGG GAGCCGCTCC GCAAGAGGCC GCGAAGAGAC 
GGCCCCGGCG TCGGGCGGAG CTCGGGCGAG CCCGGTGGGA CGGCCCCCGA GCGGGAACTG 
CCGGCGGCGG CCGGCAGTTG CCCGGCGGCG GCGGCGGCGC TGTGGCGGGA GGCCCAGGCG 
GCGGCGGCGG CGGCGGCCGA AGAAGAAGAC AATGGGCCGG GCTTACAGGG CCTATCCAGG 
GAGGCGCCAC CGGCCGACGA CTTCTACGAC GACGACGACG AGGGCGAGGA GGAGGAAGAG 
GCGGCGGCCG CTATTGGGTA CCGAGATAAC CTTCTGTTTG GTGATGAAAT CATCACCAAC 
GGTTTCCATT CGTGTGAAAG TGACGAAGAT GATAGAGCCT CGCATGCGAG TTCTAGTGAC 
TGGACTCCAA GACCTCGGAT AGGTCCATAT ACTTTTGTTC AGCAACATCT CATGATTGGC 
ACAGATCCTC GAACAATTCT TAAAGATTTA CTACCAGAAA CAATTCCTCC ACCTGAATTG 
GATGATATGA CACTGTGGCA GATTGTTATT AATATCCTTT CAGAACCACC AAAAAGGAAA 
AAAAGAAAAG ATATTAATAC AATTGAAGAT GCTGTGAAAT TACTGCAAGA GTGCAAAAAA 
ATAATAGTTC TAACTGGAGC TGGGGTTTCT GTTTCTTGTG GAATACCTGA CTTCAGGTCA 
AGAGACGGTA TTTATGCTCG CCTTGCAATA GACTTTCCAG ACCTTCCAGA TCCTCAAGCA 
ATGTTTGATA TTGAATATTT CAGAAAAGAC CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
ATATATCCTG GACAATTCCA ACCATCTCTT TGTCACAAAT TCATAGCCTT GTCTGATAAG 
GAAGGAAAAC TACTTCGCAA CTATACTCAG AACATAGATA CACTGGAGCA GGTTGCAGGA 
ATCCAAAAGA TAATTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA 
TATAAAGTTG ACTGTGAAGC TGTACGAGGA GATATTTTTA ATCAGGTGGT TCCTCGATGT 
CCTAGATGCC CAGCTGATGA ACCGCTTGCT ATCATGAAAC CAGAGATTGT CTTTTTTGGT 
GAAAATTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGATCTT 
CTCATTGTTA TTGGATCTTC CCTGAAAGTA AGACCAGTAG CACTAATTCC AAATAATCAG 
TATCTGTTTT TACCACCAAA CCGTTACATT TTCCATGGCG CTGAGGTATA TTCAGACTCT 
GAAGATGATG TCTTATCCTC TAGTTCTTGC GGCAGTAACA GTGATAGTGG AACGTGCCAG 
AGTCCAAGTT TAGAAGAACC CATGGAGGAT GAAAGTGAGA ATGAAGAATT TTACAATGGT 
TTGGAAGATG ATGCTGATGT TAATGAGAGA GCTGGAGGAA CTGTATTTGA AGCTGATGGA 
GGTGATCAAG AGGCAATTAA TGAAGCTATA TCTGTGAAAC AGGAAGCAAC ATGCATTAAC 
TATCCATCAA ACAAATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT1 ( NM_001192980.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179909.1 Bos taurus sirtuin 1 (SIRT1), mRNA.

Price & Availability↑

CloneID	OBa01331
Clone ID Related Accession (Same CDS sequence)	NM_001192980.1
Accession Version	NM_001192980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-23
Organism	Bos taurus(Cattle)
Product	NAD-dependent protein deacetylase sirtuin-1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03009214.1. On Jul 17, 2010 this sequence version replaced XM_864818.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00000728, SAMN00120238 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACG AGGCGGCGCT CGCCCTTCAG CCCGGCGGCT CTCCCTCGGT AGTGGCGGCT 
GAGAGGGAGG CCCCGTCGCC GCCTGCTGGG GAGCCGCTCC GCAAGAGGCC GCGAAGAGAC 
GGCCCCGGCG TCGGGCGGAG CTCGGGCGAG CCCGGTGGGA CGGCCCCCGA GCGGGAACTG 
CCGGCGGCGG CCGGCAGTTG CCCGGCGGCG GCGGCGGCGC TGTGGCGGGA GGCCCAGGCG 
GCGGCGGCGG CGGCGGCCGA AGAAGAAGAC AATGGGCCGG GCTTACAGGG CCTATCCAGG 
GAGGCGCCAC CGGCCGACGA CTTCTACGAC GACGACGACG AGGGCGAGGA GGAGGAAGAG 
GCGGCGGCCG CTATTGGGTA CCGAGATAAC CTTCTGTTTG GTGATGAAAT CATCACCAAC 
GGTTTCCATT CGTGTGAAAG TGACGAAGAT GATAGAGCCT CGCATGCGAG TTCTAGTGAC 
TGGACTCCAA GACCTCGGAT AGGTCCATAT ACTTTTGTTC AGCAACATCT CATGATTGGC 
ACAGATCCTC GAACAATTCT TAAAGATTTA CTACCAGAAA CAATTCCTCC ACCTGAATTG 
GATGATATGA CACTGTGGCA GATTGTTATT AATATCCTTT CAGAACCACC AAAAAGGAAA 
AAAAGAAAAG ATATTAATAC AATTGAAGAT GCTGTGAAAT TACTGCAAGA GTGCAAAAAA 
ATAATAGTTC TAACTGGAGC TGGGGTTTCT GTTTCTTGTG GAATACCTGA CTTCAGGTCA 
AGAGACGGTA TTTATGCTCG CCTTGCAATA GACTTTCCAG ACCTTCCAGA TCCTCAAGCA 
ATGTTTGATA TTGAATATTT CAGAAAAGAC CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
ATATATCCTG GACAATTCCA ACCATCTCTT TGTCACAAAT TCATAGCCTT GTCTGATAAG 
GAAGGAAAAC TACTTCGCAA CTATACTCAG AACATAGATA CACTGGAGCA GGTTGCAGGA 
ATCCAAAAGA TAATTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA 
TATAAAGTTG ACTGTGAAGC TGTACGAGGA GATATTTTTA ATCAGGTGGT TCCTCGATGT 
CCTAGATGCC CAGCTGATGA ACCGCTTGCT ATCATGAAAC CAGAAATTGT CTTTTTTGGT 
GAAAATTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGATCTT 
CTCATTGTTA TTGGATCTTC CCTGAAAGTA AGACCAGTAG CACTAATTCC AAGTTCCATA 
CCCCATGAAG TGCCTCAGAT ATTAATTAAT AGGGAACCTT TGCCTCACCT GCATTTTGAT 
GTAGAGCTTC TTGGAGACTG TGACGTAATT ATTAATGAAC TGTGTCATAG GTTAGGTGGT 
GAATATGCCA AACTTTGCTG TAACCCTGTG AAGCTTTCAG AAATTACTGA AAAACCTCCT 
CGAATACAAA AAGAGTTGGC ACATTTGTCA GAATTGCCAC CCACACCCCT CAATATTTCA 
GAAGGCTCAA GTTCACCAGA AAGAACTTCA CCACCAGATT CTTCAGTGAT TGTCACTCTT 
TTAGACCAAG AAACAAAGAG TAACGTTGAT GATCCAGATG TGTCTGAATC AAAAGACCAT 
GTCACAGAAA AATCACAGGA AGTACAGACT TCTACTAGGA GCATTGAAAG TGTTAATGAA 
CAGTTGGAGA GTCCAGATTT GAAGAATGCT GTCTCCAATT CTGGTGAGAA AAATGAAAGA 
ACTTCAGTAG CTGAAACAGT GAGAAAATGC TGGCCAGCTA GACTTGCAAA GGAGCAGATT 
AGTAAACGCC TTGATGATAA TCAGTATCTG TTTTTACCAC CAAACCGTTA CATTTTCCAT 
GGCGCTGAGG TATATTCAGA CTCTGAAGAT GATGTCTTAT CCTCTAGTTC TTGCGGCAGT 
AACAGTGATA GTGGAACGTG CCAGAGTCCA AGTTTAGAAG AACCCATGGA GGATGAAAGT 
GAGAATGAAG AATTTTACAA TGGTTTGGAA GATGATGCTG ATGTTAATGA GAGAGCTGGA 
GGAACTGTAT TTGAAGCTGA TGGAGGTGAT CAAGAGGCAA TTAATGAAGC TATATCTGTG 
AAACAGGAAG CAACATGCAT TAACTATCCA TCAAACAAAT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179909.1
CDS	34..2238
Translation	MADEAALALQPGGSPSVVAAEREAPSPPAGEPLRKRPRRDGPGVGRSSGEPGGTAPERELPAAAGSCPAAAAALWREAQAAAAAAAEEEDNGPGLQGLSREAPPADDFYDDDDEGEEEEEAAAAIGYRDNLLFGDEIITNGFHSCESDEDDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAIDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQKIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRIQKELAHLSELPPTPLNISEGSSSPERTSPPDSSVIVTLLDQETKSNVDDPDVSESKDHVTEKSQEVQTSTRSIESVNEQLESPDLKNAVSNSGEKNERTSVAETVRKCWPARLAKEQISKRLDDNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESENEEFYNGLEDDADVNERAGGTVFEADGGDQEAINEAISVKQEATCINYPSNKS

Target ORF information:

RefSeq Version	NM_001192980.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus sirtuin 1 (SIRT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192980.1

ORF Insert Sequence:

ATGGCGGACG AGGCGGCGCT CGCCCTTCAG CCCGGCGGCT CTCCCTCGGT AGTGGCGGCT 
GAGAGGGAGG CCCCGTCGCC GCCTGCTGGG GAGCCGCTCC GCAAGAGGCC GCGAAGAGAC 
GGCCCCGGCG TCGGGCGGAG CTCGGGCGAG CCCGGTGGGA CGGCCCCCGA GCGGGAACTG 
CCGGCGGCGG CCGGCAGTTG CCCGGCGGCG GCGGCGGCGC TGTGGCGGGA GGCCCAGGCG 
GCGGCGGCGG CGGCGGCCGA AGAAGAAGAC AATGGGCCGG GCTTACAGGG CCTATCCAGG 
GAGGCGCCAC CGGCCGACGA CTTCTACGAC GACGACGACG AGGGCGAGGA GGAGGAAGAG 
GCGGCGGCCG CTATTGGGTA CCGAGATAAC CTTCTGTTTG GTGATGAAAT CATCACCAAC 
GGTTTCCATT CGTGTGAAAG TGACGAAGAT GATAGAGCCT CGCATGCGAG TTCTAGTGAC 
TGGACTCCAA GACCTCGGAT AGGTCCATAT ACTTTTGTTC AGCAACATCT CATGATTGGC 
ACAGATCCTC GAACAATTCT TAAAGATTTA CTACCAGAAA CAATTCCTCC ACCTGAATTG 
GATGATATGA CACTGTGGCA GATTGTTATT AATATCCTTT CAGAACCACC AAAAAGGAAA 
AAAAGAAAAG ATATTAATAC AATTGAAGAT GCTGTGAAAT TACTGCAAGA GTGCAAAAAA 
ATAATAGTTC TAACTGGAGC TGGGGTTTCT GTTTCTTGTG GAATACCTGA CTTCAGGTCA 
AGAGACGGTA TTTATGCTCG CCTTGCAATA GACTTTCCAG ACCTTCCAGA TCCTCAAGCA 
ATGTTTGATA TTGAATATTT CAGAAAAGAC CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
ATATATCCTG GACAATTCCA ACCATCTCTT TGTCACAAAT TCATAGCCTT GTCTGATAAG 
GAAGGAAAAC TACTTCGCAA CTATACTCAG AACATAGATA CACTGGAGCA GGTTGCAGGA 
ATCCAAAAGA TAATTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA 
TATAAAGTTG ACTGTGAAGC TGTACGAGGA GATATTTTTA ATCAGGTGGT TCCTCGATGT 
CCTAGATGCC CAGCTGATGA ACCGCTTGCT ATCATGAAAC CAGAAATTGT CTTTTTTGGT 
GAAAATTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGATCTT 
CTCATTGTTA TTGGATCTTC CCTGAAAGTA AGACCAGTAG CACTAATTCC AAGTTCCATA 
CCCCATGAAG TGCCTCAGAT ATTAATTAAT AGGGAACCTT TGCCTCACCT GCATTTTGAT 
GTAGAGCTTC TTGGAGACTG TGACGTAATT ATTAATGAAC TGTGTCATAG GTTAGGTGGT 
GAATATGCCA AACTTTGCTG TAACCCTGTG AAGCTTTCAG AAATTACTGA AAAACCTCCT 
CGAATACAAA AAGAGTTGGC ACATTTGTCA GAATTGCCAC CCACACCCCT CAATATTTCA 
GAAGGCTCAA GTTCACCAGA AAGAACTTCA CCACCAGATT CTTCAGTGAT TGTCACTCTT 
TTAGACCAAG AAACAAAGAG TAACGTTGAT GATCCAGATG TGTCTGAATC AAAAGACCAT 
GTCACAGAAA AATCACAGGA AGTACAGACT TCTACTAGGA GCATTGAAAG TGTTAATGAA 
CAGTTGGAGA GTCCAGATTT GAAGAATGCT GTCTCCAATT CTGGTGAGAA AAATGAAAGA 
ACTTCAGTAG CTGAAACAGT GAGAAAATGC TGGCCAGCTA GACTTGCAAA GGAGCAGATT 
AGTAAACGCC TTGATGATAA TCAGTATCTG TTTTTACCAC CAAACCGTTA CATTTTCCAT 
GGCGCTGAGG TATATTCAGA CTCTGAAGAT GATGTCTTAT CCTCTAGTTC TTGCGGCAGT 
AACAGTGATA GTGGAACGTG CCAGAGTCCA AGTTTAGAAG AACCCATGGA GGATGAAAGT 
GAGAATGAAG AATTTTACAA TGGTTTGGAA GATGATGCTG ATGTTAATGA GAGAGCTGGA 
GGAACTGTAT TTGAAGCTGA TGGAGGTGAT CAAGAGGCAA TTAATGAAGC TATATCTGTG 
AAACAGGAAG CAACATGCAT TAACTATCCA TCAAACAAAT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIRT1 ( NM_001192980.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179909.2 Bos taurus sirtuin 1 (SIRT1), mRNA.

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CloneID	OBa216304
Clone ID Related Accession (Same CDS sequence)	NM_001192980.2
Accession Version	NM_001192980.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	NAD-dependent protein deacetylase sirtuin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02061808.1. On Jun 30, 2017 this sequence version replaced NM_001192980.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00120238, SAMN02403639 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACG AGGCGGCGCT CGCCCTTCAG CCCGGCGGCT CTCCCTCGGT AGTGGCGGCT 
GAGAGGGAGG CCCCGTCGCC GCCTGCTGGG GAGCCGCTCC GCAAGAGGCC GCGAAGAGAC 
GGCCCCGGCG TCGGGCGGAG CTCGGGCGAG CCCGGTGGGA CGGCCCCCGA GCGGGAACTG 
CCGGCGGCGG CCGGCAGTTG CCCGGCGGCG GCGGCGGCGC TGTGGCGGGA GGCCCAGGCG 
GCGGCGGCGG CGGCGGCCGA AGAAGAAGAC AATGGGCCGG GCTTACAGGG CCTATCCAGG 
GAGGCGCCAC CGGCCGACGA CTTCTACGAC GACGACGACG AGGGCGAGGA GAAGGAAGAG 
GCGGCGGCCG CTATTGGGTA CCGAGATAAC CTTCTGTTTG GTGATGAAAT CATCACCAAC 
GGTTTCCATT CGTGTGAAAG TGACGAAGAT GATAGAGCCT CGCATGCGAG TTCTAGTGAC 
TGGACTCCAA GACCTCGGAT AGGTCCATAT ACTTTTGTTC AGCAACATCT CATGATTGGC 
ACAGATCCTC GAACAATTCT TAAAGATTTA CTACCAGAAA CAATTCCTCC ACCTGAATTG 
GATGATATGA CACTGTGGCA GATTGTTATT AATATCCTTT CAGAACCACC AAAAAGGAAA 
AAAAGAAAAG ATATTAATAC AATTGAAGAT GCTGTGAAAT TACTGCAAGA GTGCAAAAAA 
ATAATAGTTC TAACTGGAGC TGGGGTTTCT GTTTCTTGTG GAATACCTGA CTTCAGGTCA 
AGAGACGGTA TTTATGCTCG CCTTGCAATA GACTTTCCAG ACCTTCCAGA TCCTCAAGCA 
ATGTTTGATA TTGAATATTT CAGAAAAGAC CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
ATATATCCTG GACAATTCCA ACCATCTCTT TGTCACAAAT TCATAGCCTT GTCTGATAAG 
GAAGGAAAAC TACTTCGCAA CTATACTCAG AACATAGATA CACTGGAGCA GGTTGCAGGA 
ATCCAAAAGA TAATTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA 
TATAAAGTTG ACTGTGAAGC TGTACGAGGA GATATTTTTA ATCAGGTGGT TCCTCGATGT 
CCTAGATGCC CAGCTGATGA ACCGCTTGCT ATCATGAAAC CAGAAATTGT CTTTTTTGGT 
GAAAATTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGATCTT 
CTCATTGTTA TTGGATCTTC CCTGAAAGTA AGACCAGTAG CACTAATTCC AAGTTCCATA 
CCCCATGAAG TGCCTCAGAT ATTAATTAAT AGGGAACCTT TGCCTCACCT GCATTTTGAT 
GTAGAGCTTC TTGGAGACTG TGACGTAATT ATTAATGAAC TGTGTCATAG GTTAGGTGGT 
GAATATGCCA AACTTTGCTG TAACCCTGTG AAGCTTTCAG AAATTACTGA AAAACCTCCT 
CGAATACAAA AAGAGTTGGC ACATTTGTCA GAATTGCCAC CCACACCCCT CAATATTTCA 
GAAGGCTCAA GTTCACCAGA AAGAACTTCA CCACCAGATT CTTCAGTGAT TGTCACTCTT 
TTAGACCAAG AAACAAAGAG TAACGTTGAT GATCCAGATG TGTCTGAATC AAAAGACCAT 
GTCACAGAAA AATCACAGGA AGTACAGACT TCTACTAGGA GCATTGAAAG TGTTAATGAA 
CAGTTGGAGA GTCCAGATTT GAAGAATGCT GTCTCCAATT CTGGTGAGAA AAATGAAAGA 
ACTTCAGTAG CTGAAACAGT GAGAAAATGC TGGCCAGCTA GACTTGCAAA GGAGCAGATT 
AGTAAACGCC TTGATGATAA TCAGTATCTG TTTTTACCAC CAAACCGTTA CATTTTCCAT 
GGCGCTGAGG TATATTCAGA CTCTGAAGAT GATGTCTTAT CCTCTAGTTC TTGCGGCAGT 
AACAGTGATA GTGGAACGTG CCAGAGTCCA AGTTTAGAAG AACCCATGGA GGATGAAAGT 
GAGAATGAAG AATTTTACAA TGGTTTGGAA GATGATGCTG ATGTTAATGA GAGAGCTGGA 
GGAACTGTAT TTGAAGCTGA TGGAGGTGAT CAAGAGGCAA TTAATGAAGC TATATCTGTG 
AAACAGGAAG CAACATGCAT TAACTATCCA TCAAACAAAT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179909.2
CDS	34..2238
Translation	MADEAALALQPGGSPSVVAAEREAPSPPAGEPLRKRPRRDGPGVGRSSGEPGGTAPERELPAAAGSCPAAAAALWREAQAAAAAAAEEEDNGPGLQGLSREAPPADDFYDDDDEGEEKEEAAAAIGYRDNLLFGDEIITNGFHSCESDEDDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAIDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQKIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRIQKELAHLSELPPTPLNISEGSSSPERTSPPDSSVIVTLLDQETKSNVDDPDVSESKDHVTEKSQEVQTSTRSIESVNEQLESPDLKNAVSNSGEKNERTSVAETVRKCWPARLAKEQISKRLDDNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESENEEFYNGLEDDADVNERAGGTVFEADGGDQEAINEAISVKQEATCINYPSNKS

Target ORF information:

RefSeq Version	NM_001192980.2
Organism	Bos taurus(cattle)
Definition	Bos taurus sirtuin 1 (SIRT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192980.2

ORF Insert Sequence:

ATGGCGGACG AGGCGGCGCT CGCCCTTCAG CCCGGCGGCT CTCCCTCGGT AGTGGCGGCT 
GAGAGGGAGG CCCCGTCGCC GCCTGCTGGG GAGCCGCTCC GCAAGAGGCC GCGAAGAGAC 
GGCCCCGGCG TCGGGCGGAG CTCGGGCGAG CCCGGTGGGA CGGCCCCCGA GCGGGAACTG 
CCGGCGGCGG CCGGCAGTTG CCCGGCGGCG GCGGCGGCGC TGTGGCGGGA GGCCCAGGCG 
GCGGCGGCGG CGGCGGCCGA AGAAGAAGAC AATGGGCCGG GCTTACAGGG CCTATCCAGG 
GAGGCGCCAC CGGCCGACGA CTTCTACGAC GACGACGACG AGGGCGAGGA GAAGGAAGAG 
GCGGCGGCCG CTATTGGGTA CCGAGATAAC CTTCTGTTTG GTGATGAAAT CATCACCAAC 
GGTTTCCATT CGTGTGAAAG TGACGAAGAT GATAGAGCCT CGCATGCGAG TTCTAGTGAC 
TGGACTCCAA GACCTCGGAT AGGTCCATAT ACTTTTGTTC AGCAACATCT CATGATTGGC 
ACAGATCCTC GAACAATTCT TAAAGATTTA CTACCAGAAA CAATTCCTCC ACCTGAATTG 
GATGATATGA CACTGTGGCA GATTGTTATT AATATCCTTT CAGAACCACC AAAAAGGAAA 
AAAAGAAAAG ATATTAATAC AATTGAAGAT GCTGTGAAAT TACTGCAAGA GTGCAAAAAA 
ATAATAGTTC TAACTGGAGC TGGGGTTTCT GTTTCTTGTG GAATACCTGA CTTCAGGTCA 
AGAGACGGTA TTTATGCTCG CCTTGCAATA GACTTTCCAG ACCTTCCAGA TCCTCAAGCA 
ATGTTTGATA TTGAATATTT CAGAAAAGAC CCAAGACCAT TCTTCAAGTT TGCAAAGGAA 
ATATATCCTG GACAATTCCA ACCATCTCTT TGTCACAAAT TCATAGCCTT GTCTGATAAG 
GAAGGAAAAC TACTTCGCAA CTATACTCAG AACATAGATA CACTGGAGCA GGTTGCAGGA 
ATCCAAAAGA TAATTCAGTG TCATGGTTCC TTTGCAACAG CATCTTGCCT GATTTGTAAA 
TATAAAGTTG ACTGTGAAGC TGTACGAGGA GATATTTTTA ATCAGGTGGT TCCTCGATGT 
CCTAGATGCC CAGCTGATGA ACCGCTTGCT ATCATGAAAC CAGAAATTGT CTTTTTTGGT 
GAAAATTTAC CAGAACAGTT TCATAGAGCC ATGAAGTATG ACAAAGATGA AGTTGATCTT 
CTCATTGTTA TTGGATCTTC CCTGAAAGTA AGACCAGTAG CACTAATTCC AAGTTCCATA 
CCCCATGAAG TGCCTCAGAT ATTAATTAAT AGGGAACCTT TGCCTCACCT GCATTTTGAT 
GTAGAGCTTC TTGGAGACTG TGACGTAATT ATTAATGAAC TGTGTCATAG GTTAGGTGGT 
GAATATGCCA AACTTTGCTG TAACCCTGTG AAGCTTTCAG AAATTACTGA AAAACCTCCT 
CGAATACAAA AAGAGTTGGC ACATTTGTCA GAATTGCCAC CCACACCCCT CAATATTTCA 
GAAGGCTCAA GTTCACCAGA AAGAACTTCA CCACCAGATT CTTCAGTGAT TGTCACTCTT 
TTAGACCAAG AAACAAAGAG TAACGTTGAT GATCCAGATG TGTCTGAATC AAAAGACCAT 
GTCACAGAAA AATCACAGGA AGTACAGACT TCTACTAGGA GCATTGAAAG TGTTAATGAA 
CAGTTGGAGA GTCCAGATTT GAAGAATGCT GTCTCCAATT CTGGTGAGAA AAATGAAAGA 
ACTTCAGTAG CTGAAACAGT GAGAAAATGC TGGCCAGCTA GACTTGCAAA GGAGCAGATT 
AGTAAACGCC TTGATGATAA TCAGTATCTG TTTTTACCAC CAAACCGTTA CATTTTCCAT 
GGCGCTGAGG TATATTCAGA CTCTGAAGAT GATGTCTTAT CCTCTAGTTC TTGCGGCAGT 
AACAGTGATA GTGGAACGTG CCAGAGTCCA AGTTTAGAAG AACCCATGGA GGATGAAAGT 
GAGAATGAAG AATTTTACAA TGGTTTGGAA GATGATGCTG ATGTTAATGA GAGAGCTGGA 
GGAACTGTAT TTGAAGCTGA TGGAGGTGAT CAAGAGGCAA TTAATGAAGC TATATCTGTG 
AAACAGGAAG CAACATGCAT TAACTATCCA TCAAACAAAT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Resveratrol induces apoptosis and inhibits adipogenesis by stimulating the SIRT1-AMPK?-FOXO1 signalling pathway in bovine intramuscular adipocytes.
Molecular and cellular biochemistry(2017 Aug)
Liu X,Zhao H,Jin Q,You W,Cheng H,Liu Y,Song E,Liu G,Tan X,Zhang X,Wan F

Haplotype distribution in the class I sirtuin genes and their associations with ultrasound carcass traits in Qinchuan cattle (Bos taurus).
Molecular and cellular probes29(3)167-71(2015 Jun)
Gui L,Hao R,Zhang Y,Zhao X,Zan L

Molecular characterization, expression profiles, and analysis of Qinchuan cattle SIRT1 gene association with meat quality and body measurement traits (Bos taurus).
Molecular biology reports41(8)5237-46(2014 Aug)
Gui L,Wang H,Wei S,Zhang Y,Zan L

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SIRT1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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