FLNB cDNA ORF clone, Bos taurus(cattle)

The following FLNB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FLNB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa216199 NM_001191460.2
Latest version!
Bos taurus filamin B (FLNB), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa229111 XM_005222904.4
Latest version!
Bos taurus filamin B (FLNB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa229112 XM_005222905.4
Latest version!
Bos taurus filamin B (FLNB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa229113 XM_005222906.4
Latest version!
Bos taurus filamin B (FLNB), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa229114 XM_005222907.4
Latest version!
Bos taurus filamin B (FLNB), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa229115 XM_005222908.4
Latest version!
Bos taurus filamin B (FLNB), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa40688 NM_001191460.3
Latest version!
Bos taurus filamin B (FLNB), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31625 XM_005222904.3
Latest version!
Bos taurus filamin B (FLNB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31626 XM_005222905.3
Latest version!
Bos taurus filamin B (FLNB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31627 XM_005222906.3
Latest version!
Bos taurus filamin B (FLNB), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31628 XM_005222907.3
Latest version!
Bos taurus filamin B (FLNB), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa31629 XM_005222908.3
Latest version!
Bos taurus filamin B (FLNB), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
OBa40688 NM_001191460.1 Bos taurus filamin B, beta (FLNB), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa216199
    Clone ID Related Accession (Same CDS sequence) NM_001191460.2
    Accession Version NM_001191460.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product filamin-B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02054297.1. On Jun 30, 2017 this sequence version replaced NM_001191460.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA
    GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG
    AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC
    CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC
    AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC
    TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA
    GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC
    GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG
    ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA
    CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA
    ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT
    TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC
    GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC
    TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC
    GCAGCTGTGA AGGAAGTACC GGTAAATGCA TGTCCCCCTG GATTCAGGCC CTGGGTAACA
    GAAGAGGCCT ATGTCCCAGT GAGCAACATG AACGGCCTGG GATTTAAGCC TTTCGACTTG
    GTCATTCCAT TTGCAGTCAG GAAAGGAGAA ATCACCGGAG AGGTCCACAT GCCTTCCGGG
    AAGACGGCCA AGCCCGAGAT TGTGGACAAC AAGGACGGCA CAGTCACTGT CAGATACGCC
    CCCACGGAGG TTGGCCTCCA CGAGATGCAC ATCAAGTACA TGGGCAGCCA CATTCCTGAG
    AGCCCCCTCC AGTTCTACGT GAACTACCCC AACAGTGGGA GCGTGTCTGC GTACGGCCCA
    GGCCTGGTGT ACGGAGTGGC CAACAAAACC GCCACCTTCA CCATCGTCAC CGAAGACGCC
    GGAGAAGGTG GCCTGGACTT GGCTATCGAG GGACCCTCAA AGGCCGAAAT CAGCTGTATT
    GACAACAAAG ATGGGACGTG CACGGTGACT TACCTGCCCA CCTTGCCGGG CGACTACAGC
    ATCCTGGTCA AGTACAATGA CAAACACATC CCTGGCAGCC CCTTCACAGC CAAGATCACA
    GACGACAGCA GACGGTGCTC TCAGGTGAAG CTGGGCTCAG CCGCCGACTT CTTGCTTGAC
    ATCAGCGAGA CTGACCTCAG CACCTTGACG GCCAGCATCA AGGCCCCGTC CGGTCGTGAC
    GAGCCTTGTC TCCTGAAGAG GCTGCCCAAC AATCACATCG GCATCTCCTT CATTCCCCGG
    GAGGTGGGTG AACACCTGGT CAGCATCAAG AAGAACGGCA ACCACGTGGC CAACAGCCCC
    GTATCCATCA TGGTGGTCCA GTCGGAGATT GGGGATGCCC GCAGAGCCAA AGTCTATGGC
    CGTGGCCTGT CAGAAGGCCG GACTTTCGAG ATGTCTGACT TCATTGTGGA CACGAGAGAT
    GCAGGTTACG GAGGCATCTC CTTGGCGGTG GAAGGCCCTA GCAAGGTGGA CATCCAGACC
    GAGGACCTGG AGGACGGCAC CTGCAAGGTC TCCTACTTCC CCACTGTGCC TGGGGTTTAT
    ATCGTCTCCA CCAAGTTCGC TGACGAGCAC GTGCCTGGGA GCCCGTTCAC CGTGAAAATC
    AGCGGGGAGG GAAGAGTCAA GGAGAGCATC ACCCGTACCA GCCGAGCCCC GTCTGTGGCC
    ACTGTTGGCA GCATCTGTGA CCTGAACCTG AAAATCCCAG AAATTGACAG CAGTGACATG
    TCGGCCCACG TCACCAGCCC CTCTGGCCGC GTGACCGAGG CAGAGATCGT GCCGGTGGGG
    AAGAACTCGC ACTGCGTCCG GTTCGTGCCC CAGGAGATGG GCGTTCACAC CGTCAGCGTC
    AAGTACCGTG GCCAGCACGT CACTGGCAGC CCCTTCCAGT TCACTGTGGG GCCGCTGGGC
    GAGGGGGGTG CCCACAAGGT CCGGGCAGGC GGCCCTGGCC TGGAGCGAGG AGAAGCCGGA
    GTTCCGGCGG AGTTCAGCAT CTGGACCCGG GAGGCAGGCG CCGGGGGCCT CTCCATCGCC
    GTGGAGGGCC CCAGTAAGGC CGAGATTACA TTCGATGACC ATAAAAACGG ATCTTGTGGT
    GTGTCTTATA TTGCCCAGGA ACCTGGTAAC TACGAGGTCT CCATCAAGTT CAATGACGAG
    CATATTCCCG AAAGTCCCTA TGTGGTGCCG GTGATCGCGC CCTCGGACGA CGCCCGCCGC
    CTCACTGTTC TGAGCCTTCA GGAATCGGGA TTAAAAGTTA ATCAGCCAGC CTCCTTTGCT
    ATAAGGTTGA ACGGGGCAAA AGGCAAGATC GATGCAAAGG TGCACAGCCC CTCTGGAGCC
    GTGGAGGAAT GCCACGTGTC TGAGCTGGAG CCAGATAAGT ATGCTGTTCG CTTCATCCCC
    CACGAGAATG GTATCCACAC GATTGACGTC AAGTTCAACG GGAGCCACGT GGTTGGAAGT
    CCCTTCAAAG TGCGCGTCGG GGAGCCTGGA CAAGCGGGGA ACCCCGCCCT GGTGTCGGCC
    TATGGCGCAG GGCTTGAGGG AGGCTCCACA GGTATCCAGT CAGAATTTTT CATCAATACC
    ACCCGAGCAG GGCCAGGGAC ATTATCCGTC ACCATCGAAG GGCCGTCCAA GGTTAAAATG
    GATTGCCAGG AAACCCCCGA AGGGTACAAG GTCATGTACA CCCCCATGGC TCCTGGAAAC
    TACCTGATTG GCGTCAAATA TGGCGGGCCC AACCACATCG TGGGCAGCCC GTTCAAGGCC
    AAGGTGACAG GCCAGCGTCT GGTCAGCCCC GGCTCGGCCA ACGAGACCTC CTCCATCCTG
    GTGGAGTCAG TGACCAGGTC CTCGACGGAG ACGTGCTACA GCGCCATCCC CAAGGCATCC
    TCAGACGCCA GCAAGGTGAC CTCCAAGGGC GCAGGGCTCT CGAAGGCCTT CCTGGGCCAG
    AAGAGCTCCT TCCTGGTGGA CTGCAGCAAA GCCGGTTCCA ACATGTTGCT GATCGGGGTC
    CACGGGCCCA CCACCCCCTG CGAAGAAGTC TCCATGAAGC ATGTGGGCAA CCAGCAGTAC
    AACGTGACCT ACGTCGTCAA GGAGCGGGGG GATTACGTCC TGGCCGTGAA GTGGGGAGAG
    GAGCACATCC CCGGCAGTCC GTTCCACGTC ACCGTGCCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178389.1
    CDS133..8034
    Translation

    Target ORF information:

    RefSeq Version NM_001191460.2
    Organism Bos taurus(cattle)
    Definition Bos taurus filamin B (FLNB), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191460.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA
    GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG
    AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC
    CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC
    AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC
    TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA
    GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC
    GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG
    ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA
    CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA
    ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT
    TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC
    GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC
    TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC
    GCAGCTGTGA AGGAAGTACC GGTAAATGCA TGTCCCCCTG GATTCAGGCC CTGGGTAACA
    GAAGAGGCCT ATGTCCCAGT GAGCAACATG AACGGCCTGG GATTTAAGCC TTTCGACTTG
    GTCATTCCAT TTGCAGTCAG GAAAGGAGAA ATCACCGGAG AGGTCCACAT GCCTTCCGGG
    AAGACGGCCA AGCCCGAGAT TGTGGACAAC AAGGACGGCA CAGTCACTGT CAGATACGCC
    CCCACGGAGG TTGGCCTCCA CGAGATGCAC ATCAAGTACA TGGGCAGCCA CATTCCTGAG
    AGCCCCCTCC AGTTCTACGT GAACTACCCC AACAGTGGGA GCGTGTCTGC GTACGGCCCA
    GGCCTGGTGT ACGGAGTGGC CAACAAAACC GCCACCTTCA CCATCGTCAC CGAAGACGCC
    GGAGAAGGTG GCCTGGACTT GGCTATCGAG GGACCCTCAA AGGCCGAAAT CAGCTGTATT
    GACAACAAAG ATGGGACGTG CACGGTGACT TACCTGCCCA CCTTGCCGGG CGACTACAGC
    ATCCTGGTCA AGTACAATGA CAAACACATC CCTGGCAGCC CCTTCACAGC CAAGATCACA
    GACGACAGCA GACGGTGCTC TCAGGTGAAG CTGGGCTCAG CCGCCGACTT CTTGCTTGAC
    ATCAGCGAGA CTGACCTCAG CACCTTGACG GCCAGCATCA AGGCCCCGTC CGGTCGTGAC
    GAGCCTTGTC TCCTGAAGAG GCTGCCCAAC AATCACATCG GCATCTCCTT CATTCCCCGG
    GAGGTGGGTG AACACCTGGT CAGCATCAAG AAGAACGGCA ACCACGTGGC CAACAGCCCC
    GTATCCATCA TGGTGGTCCA GTCGGAGATT GGGGATGCCC GCAGAGCCAA AGTCTATGGC
    CGTGGCCTGT CAGAAGGCCG GACTTTCGAG ATGTCTGACT TCATTGTGGA CACGAGAGAT
    GCAGGTTACG GAGGCATCTC CTTGGCGGTG GAAGGCCCTA GCAAGGTGGA CATCCAGACC
    GAGGACCTGG AGGACGGCAC CTGCAAGGTC TCCTACTTCC CCACTGTGCC TGGGGTTTAT
    ATCGTCTCCA CCAAGTTCGC TGACGAGCAC GTGCCTGGGA GCCCGTTCAC CGTGAAAATC
    AGCGGGGAGG GAAGAGTCAA GGAGAGCATC ACCCGTACCA GCCGAGCCCC GTCTGTGGCC
    ACTGTTGGCA GCATCTGTGA CCTGAACCTG AAAATCCCAG AAATTGACAG CAGTGACATG
    TCGGCCCACG TCACCAGCCC CTCTGGCCGC GTGACCGAGG CAGAGATCGT GCCGGTGGGG
    AAGAACTCGC ACTGCGTCCG GTTCGTGCCC CAGGAGATGG GCGTTCACAC CGTCAGCGTC
    AAGTACCGTG GCCAGCACGT CACTGGCAGC CCCTTCCAGT TCACTGTGGG GCCGCTGGGC
    GAGGGGGGTG CCCACAAGGT CCGGGCAGGC GGCCCTGGCC TGGAGCGAGG AGAAGCCGGA
    GTTCCGGCGG AGTTCAGCAT CTGGACCCGG GAGGCAGGCG CCGGGGGCCT CTCCATCGCC
    GTGGAGGGCC CCAGTAAGGC CGAGATTACA TTCGATGACC ATAAAAACGG ATCTTGTGGT
    GTGTCTTATA TTGCCCAGGA ACCTGGTAAC TACGAGGTCT CCATCAAGTT CAATGACGAG
    CATATTCCCG AAAGTCCCTA TGTGGTGCCG GTGATCGCGC CCTCGGACGA CGCCCGCCGC
    CTCACTGTTC TGAGCCTTCA GGAATCGGGA TTAAAAGTTA ATCAGCCAGC CTCCTTTGCT
    ATAAGGTTGA ACGGGGCAAA AGGCAAGATC GATGCAAAGG TGCACAGCCC CTCTGGAGCC
    GTGGAGGAAT GCCACGTGTC TGAGCTGGAG CCAGATAAGT ATGCTGTTCG CTTCATCCCC
    CACGAGAATG GTATCCACAC GATTGACGTC AAGTTCAACG GGAGCCACGT GGTTGGAAGT
    CCCTTCAAAG TGCGCGTCGG GGAGCCTGGA CAAGCGGGGA ACCCCGCCCT GGTGTCGGCC
    TATGGCGCAG GGCTTGAGGG AGGCTCCACA GGTATCCAGT CAGAATTTTT CATCAATACC
    ACCCGAGCAG GGCCAGGGAC ATTATCCGTC ACCATCGAAG GGCCGTCCAA GGTTAAAATG
    GATTGCCAGG AAACCCCCGA AGGGTACAAG GTCATGTACA CCCCCATGGC TCCTGGAAAC
    TACCTGATTG GCGTCAAATA TGGCGGGCCC AACCACATCG TGGGCAGCCC GTTCAAGGCC
    AAGGTGACAG GCCAGCGTCT GGTCAGCCCC GGCTCGGCCA ACGAGACCTC CTCCATCCTG
    GTGGAGTCAG TGACCAGGTC CTCGACGGAG ACGTGCTACA GCGCCATCCC CAAGGCATCC
    TCAGACGCCA GCAAGGTGAC CTCCAAGGGC GCAGGGCTCT CGAAGGCCTT CCTGGGCCAG
    AAGAGCTCCT TCCTGGTGGA CTGCAGCAAA GCCGGTTCCA ACATGTTGCT GATCGGGGTC
    CACGGGCCCA CCACCCCCTG CGAAGAAGTC TCCATGAAGC ATGTGGGCAA CCAGCAGTAC
    AACGTGACCT ACGTCGTCAA GGAGCGGGGG GATTACGTCC TGGCCGTGAA GTGGGGAGAG
    GAGCACATCC CCGGCAGTCC GTTCCACGTC ACCGTGCCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229111
    Clone ID Related Accession (Same CDS sequence) XM_005222904.4
    Accession Version XM_005222904.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product filamin-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222904.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA
    GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG
    AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC
    CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC
    AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC
    TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA
    GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC
    GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG
    ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA
    CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA
    ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT
    TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC
    GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC
    TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC
    GCAGCTGTGA AGGAAGTACC GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC
    GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC
    ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG
    GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC
    AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC
    AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC
    ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA
    CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC
    CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT
    GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG
    GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC
    AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT
    CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG
    AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG
    GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG
    TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA
    GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC
    TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG
    CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC
    CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA
    ATCCCAGAAA TTGACAGCAG TGACATGTCA GCCCACGTCA CCAGCCCCTC TGGCCGCGTG
    ACCGAGGCGG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG
    GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC
    TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC
    CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG
    GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC
    GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC
    GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG
    ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA
    AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT
    GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA
    GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG
    TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA
    GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT
    ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC
    ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC
    ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC
    CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC
    TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG
    TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA
    GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC
    GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC
    ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT
    TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC
    GTGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222961.1
    CDS178..8046
    Translation

    Target ORF information:

    RefSeq Version XM_005222904.4
    Organism Bos taurus(cattle)
    Definition Bos taurus filamin B (FLNB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222904.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA
    GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG
    AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC
    CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC
    AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC
    TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA
    GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC
    GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG
    ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA
    CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA
    ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT
    TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC
    GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC
    TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC
    GCAGCTGTGA AGGAAGTACC GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC
    GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC
    ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG
    GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC
    AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC
    AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC
    ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA
    CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC
    CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT
    GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG
    GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC
    AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT
    CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG
    AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG
    GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG
    TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA
    GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC
    TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG
    CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC
    CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA
    ATCCCAGAAA TTGACAGCAG TGACATGTCA GCCCACGTCA CCAGCCCCTC TGGCCGCGTG
    ACCGAGGCGG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG
    GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC
    TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC
    CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG
    GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC
    GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC
    GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG
    ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA
    AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT
    GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA
    GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG
    TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA
    GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT
    ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC
    ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC
    ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC
    CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC
    TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG
    TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA
    GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC
    GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC
    ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT
    TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC
    GTGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229112
    Clone ID Related Accession (Same CDS sequence) XM_005222905.4
    Accession Version XM_005222905.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product filamin-B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222905.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA
    GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG
    AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC
    CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC
    AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC
    TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA
    GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC
    GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG
    ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA
    CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA
    ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT
    TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC
    GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC
    TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGTAACAGA AGAGGCCTAT
    GTCCCAGTGA GCAACATGAA CGGCCTGGGA TTTAAGCCTT TCGACTTGGT CATTCCATTT
    GCAGTCAGGA AAGGAGAAAT CACCGGAGAG GTCCACATGC CTTCCGGGAA GACGGCCAAG
    CCCGAGATTG TGGACAACAA GGACGGCACA GTCACTGTCA GATACGCCCC CACGGAGGTT
    GGCCTCCACG AGATGCACAT CAAGTACATG GGCAGCCACA TTCCTGAGAG CCCCCTCCAG
    TTCTACGTGA ACTACCCCAA CAGTGGGAGC GTGTCTGCGT ACGGCCCAGG CCTGGTGTAC
    GGAGTGGCCA ACAAAACCGC CACCTTCACC ATCGTCACCG AAGACGCCGG AGAAGGTGGC
    CTGGACTTGG CTATCGAGGG ACCCTCAAAG GCCGAAATCA GCTGTATTGA CAACAAAGAT
    GGGACGTGCA CGGTGACTTA CCTGCCCACC TTGCCGGGCG ACTACAGCAT CCTGGTCAAG
    TACAATGACA AACACATCCC TGGCAGCCCC TTCACAGCCA AGATCACAGA CGACAGCAGA
    CGGTGCTCTC AGGTGAAGCT GGGCTCAGCC GCCGACTTCT TGCTTGACAT CAGCGAGACT
    GACCTCAGCA CCTTGACGGC CAGCATCAAG GCCCCGTCCG GTCGTGACGA GCCTTGTCTC
    CTGAAGAGGC TGCCCAACAA TCACATCGGC ATCTCCTTCA TTCCCCGGGA GGTGGGTGAA
    CACCTGGTCA GCATCAAGAA GAACGGCAAC CACGTGGCCA ACAGCCCCGT ATCCATCATG
    GTGGTCCAGT CGGAGATTGG GGATGCCCGC AGAGCCAAAG TCTATGGCCG TGGCCTGTCA
    GAAGGCCGGA CTTTCGAGAT GTCTGACTTC ATTGTGGACA CGAGAGATGC AGGTTACGGA
    GGCATCTCCT TGGCGGTGGA AGGCCCTAGC AAGGTGGACA TCCAGACCGA GGACCTGGAG
    GACGGCACCT GCAAGGTCTC CTACTTCCCC ACTGTGCCTG GGGTTTATAT CGTCTCCACC
    AAGTTCGCTG ACGAGCACGT GCCTGGGAGC CCGTTCACCG TGAAAATCAG CGGGGAGGGA
    AGAGTCAAGG AGAGCATCAC CCGTACCAGC CGAGCCCCGT CTGTGGCCAC TGTTGGCAGC
    ATCTGTGACC TGAACCTGAA AATCCCAGAA ATTGACAGCA GTGACATGTC AGCCCACGTC
    ACCAGCCCCT CTGGCCGCGT GACCGAGGCG GAGATCGTGC CGGTGGGGAA GAACTCGCAC
    TGCGTCCGGT TCGTGCCCCA GGAGATGGGC GTTCACACCG TCAGCGTCAA GTACCGTGGC
    CAGCACGTCA CTGGCAGCCC CTTCCAGTTC ACTGTGGGGC CGCTGGGCGA GGGGGGTGCC
    CACAAGGTCC GGGCAGGCGG CCCTGGCCTG GAGCGAGGAG AAGCCGGAGT TCCGGCGGAG
    TTCAGCATCT GGACCCGGGA GGCAGGCGCC GGGGGCCTCT CCATCGCCGT GGAGGGCCCC
    AGTAAGGCCG AGATTACATT CGATGACCAT AAAAACGGAT CTTGTGGTGT GTCTTATATT
    GCCCAGGAAC CTGGTAACTA CGAGGTCTCC ATCAAGTTCA ATGACGAGCA TATTCCCGAA
    AGTCCCTATG TGGTGCCGGT GATCGCGCCC TCGGACGACG CCCGCCGCCT CACTGTTCTG
    AGCCTTCAGG AATCGGGATT AAAAGTTAAT CAGCCAGCCT CCTTTGCTAT AAGGTTGAAC
    GGGGCAAAAG GCAAGATCGA TGCAAAGGTG CACAGCCCCT CTGGAGCCGT GGAGGAATGC
    CACGTGTCTG AGCTGGAGCC AGATAAGTAT GCTGTTCGCT TCATCCCCCA CGAGAATGGT
    ATCCACACGA TTGACGTCAA GTTCAACGGG AGCCACGTGG TTGGAAGTCC CTTCAAAGTG
    CGCGTCGGGG AGCCTGGACA AGCGGGGAAC CCCGCCCTGG TGTCGGCCTA TGGCGCAGGG
    CTTGAGGGAG GCTCCACAGG TATCCAGTCA GAATTTTTCA TCAATACCAC CCGAGCAGGG
    CCAGGGACAT TATCCGTCAC CATCGAAGGG CCGTCCAAGG TTAAAATGGA TTGCCAGGAA
    ACCCCCGAAG GGTACAAGGT CATGTACACC CCCATGGCTC CTGGAAACTA CCTGATTGGC
    GTCAAATATG GCGGGCCCAA CCACATCGTG GGCAGCCCGT TCAAGGCCAA GGTGACAGGC
    CAGCGTCTGG TCAGCCCCGG CTCGGCCAAC GAGACCTCCT CCATCCTGGT GGAGTCAGTG
    ACCAGGTCCT CGACGGAGAC GTGCTACAGC GCCATCCCCA AGGCATCCTC AGACGCCAGC
    AAGGTGACCT CCAAGGGCGC AGGGCTCTCG AAGGCCTTCC TGGGCCAGAA GAGCTCCTTC
    CTGGTGGACT GCAGCAAAGC CGGTTCCAAC ATGTTGCTGA TCGGGGTCCA CGGGCCCACC
    ACCCCCTGCG AAGAAGTCTC CATGAAGCAT GTGGGCAACC AGCAGTACAA CGTGACCTAC
    GTCGTCAAGG AGCGGGGGGA TTACGTCCTG GCCGTGAAGT GGGGAGAGGA GCACATCCCC
    GGCAGTCCGT TCCACGTCAC CGTGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222962.1
    CDS178..8007
    Translation

    Target ORF information:

    RefSeq Version XM_005222905.4
    Organism Bos taurus(cattle)
    Definition Bos taurus filamin B (FLNB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222905.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA
    GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG
    AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC
    CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC
    AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC
    TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA
    GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC
    GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG
    ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA
    CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA
    ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT
    TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC
    GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC
    TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGTAACAGA AGAGGCCTAT
    GTCCCAGTGA GCAACATGAA CGGCCTGGGA TTTAAGCCTT TCGACTTGGT CATTCCATTT
    GCAGTCAGGA AAGGAGAAAT CACCGGAGAG GTCCACATGC CTTCCGGGAA GACGGCCAAG
    CCCGAGATTG TGGACAACAA GGACGGCACA GTCACTGTCA GATACGCCCC CACGGAGGTT
    GGCCTCCACG AGATGCACAT CAAGTACATG GGCAGCCACA TTCCTGAGAG CCCCCTCCAG
    TTCTACGTGA ACTACCCCAA CAGTGGGAGC GTGTCTGCGT ACGGCCCAGG CCTGGTGTAC
    GGAGTGGCCA ACAAAACCGC CACCTTCACC ATCGTCACCG AAGACGCCGG AGAAGGTGGC
    CTGGACTTGG CTATCGAGGG ACCCTCAAAG GCCGAAATCA GCTGTATTGA CAACAAAGAT
    GGGACGTGCA CGGTGACTTA CCTGCCCACC TTGCCGGGCG ACTACAGCAT CCTGGTCAAG
    TACAATGACA AACACATCCC TGGCAGCCCC TTCACAGCCA AGATCACAGA CGACAGCAGA
    CGGTGCTCTC AGGTGAAGCT GGGCTCAGCC GCCGACTTCT TGCTTGACAT CAGCGAGACT
    GACCTCAGCA CCTTGACGGC CAGCATCAAG GCCCCGTCCG GTCGTGACGA GCCTTGTCTC
    CTGAAGAGGC TGCCCAACAA TCACATCGGC ATCTCCTTCA TTCCCCGGGA GGTGGGTGAA
    CACCTGGTCA GCATCAAGAA GAACGGCAAC CACGTGGCCA ACAGCCCCGT ATCCATCATG
    GTGGTCCAGT CGGAGATTGG GGATGCCCGC AGAGCCAAAG TCTATGGCCG TGGCCTGTCA
    GAAGGCCGGA CTTTCGAGAT GTCTGACTTC ATTGTGGACA CGAGAGATGC AGGTTACGGA
    GGCATCTCCT TGGCGGTGGA AGGCCCTAGC AAGGTGGACA TCCAGACCGA GGACCTGGAG
    GACGGCACCT GCAAGGTCTC CTACTTCCCC ACTGTGCCTG GGGTTTATAT CGTCTCCACC
    AAGTTCGCTG ACGAGCACGT GCCTGGGAGC CCGTTCACCG TGAAAATCAG CGGGGAGGGA
    AGAGTCAAGG AGAGCATCAC CCGTACCAGC CGAGCCCCGT CTGTGGCCAC TGTTGGCAGC
    ATCTGTGACC TGAACCTGAA AATCCCAGAA ATTGACAGCA GTGACATGTC AGCCCACGTC
    ACCAGCCCCT CTGGCCGCGT GACCGAGGCG GAGATCGTGC CGGTGGGGAA GAACTCGCAC
    TGCGTCCGGT TCGTGCCCCA GGAGATGGGC GTTCACACCG TCAGCGTCAA GTACCGTGGC
    CAGCACGTCA CTGGCAGCCC CTTCCAGTTC ACTGTGGGGC CGCTGGGCGA GGGGGGTGCC
    CACAAGGTCC GGGCAGGCGG CCCTGGCCTG GAGCGAGGAG AAGCCGGAGT TCCGGCGGAG
    TTCAGCATCT GGACCCGGGA GGCAGGCGCC GGGGGCCTCT CCATCGCCGT GGAGGGCCCC
    AGTAAGGCCG AGATTACATT CGATGACCAT AAAAACGGAT CTTGTGGTGT GTCTTATATT
    GCCCAGGAAC CTGGTAACTA CGAGGTCTCC ATCAAGTTCA ATGACGAGCA TATTCCCGAA
    AGTCCCTATG TGGTGCCGGT GATCGCGCCC TCGGACGACG CCCGCCGCCT CACTGTTCTG
    AGCCTTCAGG AATCGGGATT AAAAGTTAAT CAGCCAGCCT CCTTTGCTAT AAGGTTGAAC
    GGGGCAAAAG GCAAGATCGA TGCAAAGGTG CACAGCCCCT CTGGAGCCGT GGAGGAATGC
    CACGTGTCTG AGCTGGAGCC AGATAAGTAT GCTGTTCGCT TCATCCCCCA CGAGAATGGT
    ATCCACACGA TTGACGTCAA GTTCAACGGG AGCCACGTGG TTGGAAGTCC CTTCAAAGTG
    CGCGTCGGGG AGCCTGGACA AGCGGGGAAC CCCGCCCTGG TGTCGGCCTA TGGCGCAGGG
    CTTGAGGGAG GCTCCACAGG TATCCAGTCA GAATTTTTCA TCAATACCAC CCGAGCAGGG
    CCAGGGACAT TATCCGTCAC CATCGAAGGG CCGTCCAAGG TTAAAATGGA TTGCCAGGAA
    ACCCCCGAAG GGTACAAGGT CATGTACACC CCCATGGCTC CTGGAAACTA CCTGATTGGC
    GTCAAATATG GCGGGCCCAA CCACATCGTG GGCAGCCCGT TCAAGGCCAA GGTGACAGGC
    CAGCGTCTGG TCAGCCCCGG CTCGGCCAAC GAGACCTCCT CCATCCTGGT GGAGTCAGTG
    ACCAGGTCCT CGACGGAGAC GTGCTACAGC GCCATCCCCA AGGCATCCTC AGACGCCAGC
    AAGGTGACCT CCAAGGGCGC AGGGCTCTCG AAGGCCTTCC TGGGCCAGAA GAGCTCCTTC
    CTGGTGGACT GCAGCAAAGC CGGTTCCAAC ATGTTGCTGA TCGGGGTCCA CGGGCCCACC
    ACCCCCTGCG AAGAAGTCTC CATGAAGCAT GTGGGCAACC AGCAGTACAA CGTGACCTAC
    GTCGTCAAGG AGCGGGGGGA TTACGTCCTG GCCGTGAAGT GGGGAGAGGA GCACATCCCC
    GGCAGTCCGT TCCACGTCAC CGTGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229113
    Clone ID Related Accession (Same CDS sequence) XM_005222906.4
    Accession Version XM_005222906.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product filamin-B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222906.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA
    GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA
    GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG
    ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT
    GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG
    GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC
    ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA
    TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG
    GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC
    AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT
    GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG
    CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC
    ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AAATGCATGT
    CCCCCTGGAT TCAGGCCCTG GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC
    GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC
    ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG
    GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC
    AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC
    AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC
    ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA
    CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC
    CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT
    GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG
    GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC
    AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT
    CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG
    AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG
    GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG
    TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA
    GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC
    TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG
    CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC
    CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA
    ATCCCAGAAA TTGACAGCAG TGACATGTCA GCCCACGTCA CCAGCCCCTC TGGCCGCGTG
    ACCGAGGCGG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG
    GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC
    TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC
    CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG
    GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC
    GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC
    GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG
    ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA
    AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT
    GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA
    GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG
    TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA
    GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT
    ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC
    ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC
    ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC
    CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC
    TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG
    TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA
    GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC
    GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC
    ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT
    TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC
    GTGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222963.1
    CDS178..7986
    Translation

    Target ORF information:

    RefSeq Version XM_005222906.4
    Organism Bos taurus(cattle)
    Definition Bos taurus filamin B (FLNB), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222906.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA
    GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA
    GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG
    ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT
    GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG
    GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC
    ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA
    TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG
    GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC
    AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT
    GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG
    CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC
    ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AAATGCATGT
    CCCCCTGGAT TCAGGCCCTG GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC
    GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC
    ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG
    GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC
    AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC
    AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC
    ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA
    CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC
    CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT
    GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG
    GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC
    AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT
    CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG
    AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG
    GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG
    TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA
    GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC
    TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG
    CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC
    CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA
    ATCCCAGAAA TTGACAGCAG TGACATGTCA GCCCACGTCA CCAGCCCCTC TGGCCGCGTG
    ACCGAGGCGG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG
    GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC
    TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC
    CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG
    GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC
    GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC
    GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG
    ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA
    AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT
    GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA
    GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG
    TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA
    GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT
    ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC
    ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC
    ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC
    CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC
    TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG
    TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA
    GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC
    GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC
    ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT
    TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC
    GTGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229114
    Clone ID Related Accession (Same CDS sequence) XM_005222907.4
    Accession Version XM_005222907.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product filamin-B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222907.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA
    GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA
    GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG
    ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT
    GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG
    GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC
    ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA
    TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG
    GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC
    AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT
    GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG
    CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC
    ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AACAGAAGAG
    GCCTATGTCC CAGTGAGCAA CATGAACGGC CTGGGATTTA AGCCTTTCGA CTTGGTCATT
    CCATTTGCAG TCAGGAAAGG AGAAATCACC GGAGAGGTCC ACATGCCTTC CGGGAAGACG
    GCCAAGCCCG AGATTGTGGA CAACAAGGAC GGCACAGTCA CTGTCAGATA CGCCCCCACG
    GAGGTTGGCC TCCACGAGAT GCACATCAAG TACATGGGCA GCCACATTCC TGAGAGCCCC
    CTCCAGTTCT ACGTGAACTA CCCCAACAGT GGGAGCGTGT CTGCGTACGG CCCAGGCCTG
    GTGTACGGAG TGGCCAACAA AACCGCCACC TTCACCATCG TCACCGAAGA CGCCGGAGAA
    GGTGGCCTGG ACTTGGCTAT CGAGGGACCC TCAAAGGCCG AAATCAGCTG TATTGACAAC
    AAAGATGGGA CGTGCACGGT GACTTACCTG CCCACCTTGC CGGGCGACTA CAGCATCCTG
    GTCAAGTACA ATGACAAACA CATCCCTGGC AGCCCCTTCA CAGCCAAGAT CACAGACGAC
    AGCAGACGGT GCTCTCAGGT GAAGCTGGGC TCAGCCGCCG ACTTCTTGCT TGACATCAGC
    GAGACTGACC TCAGCACCTT GACGGCCAGC ATCAAGGCCC CGTCCGGTCG TGACGAGCCT
    TGTCTCCTGA AGAGGCTGCC CAACAATCAC ATCGGCATCT CCTTCATTCC CCGGGAGGTG
    GGTGAACACC TGGTCAGCAT CAAGAAGAAC GGCAACCACG TGGCCAACAG CCCCGTATCC
    ATCATGGTGG TCCAGTCGGA GATTGGGGAT GCCCGCAGAG CCAAAGTCTA TGGCCGTGGC
    CTGTCAGAAG GCCGGACTTT CGAGATGTCT GACTTCATTG TGGACACGAG AGATGCAGGT
    TACGGAGGCA TCTCCTTGGC GGTGGAAGGC CCTAGCAAGG TGGACATCCA GACCGAGGAC
    CTGGAGGACG GCACCTGCAA GGTCTCCTAC TTCCCCACTG TGCCTGGGGT TTATATCGTC
    TCCACCAAGT TCGCTGACGA GCACGTGCCT GGGAGCCCGT TCACCGTGAA AATCAGCGGG
    GAGGGAAGAG TCAAGGAGAG CATCACCCGT ACCAGCCGAG CCCCGTCTGT GGCCACTGTT
    GGCAGCATCT GTGACCTGAA CCTGAAAATC CCAGAAATTG ACAGCAGTGA CATGTCAGCC
    CACGTCACCA GCCCCTCTGG CCGCGTGACC GAGGCGGAGA TCGTGCCGGT GGGGAAGAAC
    TCGCACTGCG TCCGGTTCGT GCCCCAGGAG ATGGGCGTTC ACACCGTCAG CGTCAAGTAC
    CGTGGCCAGC ACGTCACTGG CAGCCCCTTC CAGTTCACTG TGGGGCCGCT GGGCGAGGGG
    GGTGCCCACA AGGTCCGGGC AGGCGGCCCT GGCCTGGAGC GAGGAGAAGC CGGAGTTCCG
    GCGGAGTTCA GCATCTGGAC CCGGGAGGCA GGCGCCGGGG GCCTCTCCAT CGCCGTGGAG
    GGCCCCAGTA AGGCCGAGAT TACATTCGAT GACCATAAAA ACGGATCTTG TGGTGTGTCT
    TATATTGCCC AGGAACCTGG TAACTACGAG GTCTCCATCA AGTTCAATGA CGAGCATATT
    CCCGAAAGTC CCTATGTGGT GCCGGTGATC GCGCCCTCGG ACGACGCCCG CCGCCTCACT
    GTTCTGAGCC TTCAGGAATC GGGATTAAAA GTTAATCAGC CAGCCTCCTT TGCTATAAGG
    TTGAACGGGG CAAAAGGCAA GATCGATGCA AAGGTGCACA GCCCCTCTGG AGCCGTGGAG
    GAATGCCACG TGTCTGAGCT GGAGCCAGAT AAGTATGCTG TTCGCTTCAT CCCCCACGAG
    AATGGTATCC ACACGATTGA CGTCAAGTTC AACGGGAGCC ACGTGGTTGG AAGTCCCTTC
    AAAGTGCGCG TCGGGGAGCC TGGACAAGCG GGGAACCCCG CCCTGGTGTC GGCCTATGGC
    GCAGGGCTTG AGGGAGGCTC CACAGGTATC CAGTCAGAAT TTTTCATCAA TACCACCCGA
    GCAGGGCCAG GGACATTATC CGTCACCATC GAAGGGCCGT CCAAGGTTAA AATGGATTGC
    CAGGAAACCC CCGAAGGGTA CAAGGTCATG TACACCCCCA TGGCTCCTGG AAACTACCTG
    ATTGGCGTCA AATATGGCGG GCCCAACCAC ATCGTGGGCA GCCCGTTCAA GGCCAAGGTG
    ACAGGCCAGC GTCTGGTCAG CCCCGGCTCG GCCAACGAGA CCTCCTCCAT CCTGGTGGAG
    TCAGTGACCA GGTCCTCGAC GGAGACGTGC TACAGCGCCA TCCCCAAGGC ATCCTCAGAC
    GCCAGCAAGG TGACCTCCAA GGGCGCAGGG CTCTCGAAGG CCTTCCTGGG CCAGAAGAGC
    TCCTTCCTGG TGGACTGCAG CAAAGCCGGT TCCAACATGT TGCTGATCGG GGTCCACGGG
    CCCACCACCC CCTGCGAAGA AGTCTCCATG AAGCATGTGG GCAACCAGCA GTACAACGTG
    ACCTACGTCG TCAAGGAGCG GGGGGATTAC GTCCTGGCCG TGAAGTGGGG AGAGGAGCAC
    ATCCCCGGCA GTCCGTTCCA CGTCACCGTG CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222964.1
    CDS178..7953
    Translation

    Target ORF information:

    RefSeq Version XM_005222907.4
    Organism Bos taurus(cattle)
    Definition Bos taurus filamin B (FLNB), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222907.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA
    GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA
    GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG
    ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT
    GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG
    GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC
    ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA
    TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG
    GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC
    AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT
    GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG
    CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC
    ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AACAGAAGAG
    GCCTATGTCC CAGTGAGCAA CATGAACGGC CTGGGATTTA AGCCTTTCGA CTTGGTCATT
    CCATTTGCAG TCAGGAAAGG AGAAATCACC GGAGAGGTCC ACATGCCTTC CGGGAAGACG
    GCCAAGCCCG AGATTGTGGA CAACAAGGAC GGCACAGTCA CTGTCAGATA CGCCCCCACG
    GAGGTTGGCC TCCACGAGAT GCACATCAAG TACATGGGCA GCCACATTCC TGAGAGCCCC
    CTCCAGTTCT ACGTGAACTA CCCCAACAGT GGGAGCGTGT CTGCGTACGG CCCAGGCCTG
    GTGTACGGAG TGGCCAACAA AACCGCCACC TTCACCATCG TCACCGAAGA CGCCGGAGAA
    GGTGGCCTGG ACTTGGCTAT CGAGGGACCC TCAAAGGCCG AAATCAGCTG TATTGACAAC
    AAAGATGGGA CGTGCACGGT GACTTACCTG CCCACCTTGC CGGGCGACTA CAGCATCCTG
    GTCAAGTACA ATGACAAACA CATCCCTGGC AGCCCCTTCA CAGCCAAGAT CACAGACGAC
    AGCAGACGGT GCTCTCAGGT GAAGCTGGGC TCAGCCGCCG ACTTCTTGCT TGACATCAGC
    GAGACTGACC TCAGCACCTT GACGGCCAGC ATCAAGGCCC CGTCCGGTCG TGACGAGCCT
    TGTCTCCTGA AGAGGCTGCC CAACAATCAC ATCGGCATCT CCTTCATTCC CCGGGAGGTG
    GGTGAACACC TGGTCAGCAT CAAGAAGAAC GGCAACCACG TGGCCAACAG CCCCGTATCC
    ATCATGGTGG TCCAGTCGGA GATTGGGGAT GCCCGCAGAG CCAAAGTCTA TGGCCGTGGC
    CTGTCAGAAG GCCGGACTTT CGAGATGTCT GACTTCATTG TGGACACGAG AGATGCAGGT
    TACGGAGGCA TCTCCTTGGC GGTGGAAGGC CCTAGCAAGG TGGACATCCA GACCGAGGAC
    CTGGAGGACG GCACCTGCAA GGTCTCCTAC TTCCCCACTG TGCCTGGGGT TTATATCGTC
    TCCACCAAGT TCGCTGACGA GCACGTGCCT GGGAGCCCGT TCACCGTGAA AATCAGCGGG
    GAGGGAAGAG TCAAGGAGAG CATCACCCGT ACCAGCCGAG CCCCGTCTGT GGCCACTGTT
    GGCAGCATCT GTGACCTGAA CCTGAAAATC CCAGAAATTG ACAGCAGTGA CATGTCAGCC
    CACGTCACCA GCCCCTCTGG CCGCGTGACC GAGGCGGAGA TCGTGCCGGT GGGGAAGAAC
    TCGCACTGCG TCCGGTTCGT GCCCCAGGAG ATGGGCGTTC ACACCGTCAG CGTCAAGTAC
    CGTGGCCAGC ACGTCACTGG CAGCCCCTTC CAGTTCACTG TGGGGCCGCT GGGCGAGGGG
    GGTGCCCACA AGGTCCGGGC AGGCGGCCCT GGCCTGGAGC GAGGAGAAGC CGGAGTTCCG
    GCGGAGTTCA GCATCTGGAC CCGGGAGGCA GGCGCCGGGG GCCTCTCCAT CGCCGTGGAG
    GGCCCCAGTA AGGCCGAGAT TACATTCGAT GACCATAAAA ACGGATCTTG TGGTGTGTCT
    TATATTGCCC AGGAACCTGG TAACTACGAG GTCTCCATCA AGTTCAATGA CGAGCATATT
    CCCGAAAGTC CCTATGTGGT GCCGGTGATC GCGCCCTCGG ACGACGCCCG CCGCCTCACT
    GTTCTGAGCC TTCAGGAATC GGGATTAAAA GTTAATCAGC CAGCCTCCTT TGCTATAAGG
    TTGAACGGGG CAAAAGGCAA GATCGATGCA AAGGTGCACA GCCCCTCTGG AGCCGTGGAG
    GAATGCCACG TGTCTGAGCT GGAGCCAGAT AAGTATGCTG TTCGCTTCAT CCCCCACGAG
    AATGGTATCC ACACGATTGA CGTCAAGTTC AACGGGAGCC ACGTGGTTGG AAGTCCCTTC
    AAAGTGCGCG TCGGGGAGCC TGGACAAGCG GGGAACCCCG CCCTGGTGTC GGCCTATGGC
    GCAGGGCTTG AGGGAGGCTC CACAGGTATC CAGTCAGAAT TTTTCATCAA TACCACCCGA
    GCAGGGCCAG GGACATTATC CGTCACCATC GAAGGGCCGT CCAAGGTTAA AATGGATTGC
    CAGGAAACCC CCGAAGGGTA CAAGGTCATG TACACCCCCA TGGCTCCTGG AAACTACCTG
    ATTGGCGTCA AATATGGCGG GCCCAACCAC ATCGTGGGCA GCCCGTTCAA GGCCAAGGTG
    ACAGGCCAGC GTCTGGTCAG CCCCGGCTCG GCCAACGAGA CCTCCTCCAT CCTGGTGGAG
    TCAGTGACCA GGTCCTCGAC GGAGACGTGC TACAGCGCCA TCCCCAAGGC ATCCTCAGAC
    GCCAGCAAGG TGACCTCCAA GGGCGCAGGG CTCTCGAAGG CCTTCCTGGG CCAGAAGAGC
    TCCTTCCTGG TGGACTGCAG CAAAGCCGGT TCCAACATGT TGCTGATCGG GGTCCACGGG
    CCCACCACCC CCTGCGAAGA AGTCTCCATG AAGCATGTGG GCAACCAGCA GTACAACGTG
    ACCTACGTCG TCAAGGAGCG GGGGGATTAC GTCCTGGCCG TGAAGTGGGG AGAGGAGCAC
    ATCCCCGGCA GTCCGTTCCA CGTCACCGTG CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229115
    Clone ID Related Accession (Same CDS sequence) XM_005222908.4
    Accession Version XM_005222908.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product filamin-B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222908.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA
    GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA
    GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG
    ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT
    GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG
    GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC
    ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA
    TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG
    GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC
    AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT
    GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG
    CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC
    ACTGTCATGG TAACAGAAGA GGCCTATGTC CCAGTGAGCA ACATGAACGG CCTGGGATTT
    AAGCCTTTCG ACTTGGTCAT TCCATTTGCA GTCAGGAAAG GAGAAATCAC CGGAGAGGTC
    CACATGCCTT CCGGGAAGAC GGCCAAGCCC GAGATTGTGG ACAACAAGGA CGGCACAGTC
    ACTGTCAGAT ACGCCCCCAC GGAGGTTGGC CTCCACGAGA TGCACATCAA GTACATGGGC
    AGCCACATTC CTGAGAGCCC CCTCCAGTTC TACGTGAACT ACCCCAACAG TGGGAGCGTG
    TCTGCGTACG GCCCAGGCCT GGTGTACGGA GTGGCCAACA AAACCGCCAC CTTCACCATC
    GTCACCGAAG ACGCCGGAGA AGGTGGCCTG GACTTGGCTA TCGAGGGACC CTCAAAGGCC
    GAAATCAGCT GTATTGACAA CAAAGATGGG ACGTGCACGG TGACTTACCT GCCCACCTTG
    CCGGGCGACT ACAGCATCCT GGTCAAGTAC AATGACAAAC ACATCCCTGG CAGCCCCTTC
    ACAGCCAAGA TCACAGACGA CAGCAGACGG TGCTCTCAGG TGAAGCTGGG CTCAGCCGCC
    GACTTCTTGC TTGACATCAG CGAGACTGAC CTCAGCACCT TGACGGCCAG CATCAAGGCC
    CCGTCCGGTC GTGACGAGCC TTGTCTCCTG AAGAGGCTGC CCAACAATCA CATCGGCATC
    TCCTTCATTC CCCGGGAGGT GGGTGAACAC CTGGTCAGCA TCAAGAAGAA CGGCAACCAC
    GTGGCCAACA GCCCCGTATC CATCATGGTG GTCCAGTCGG AGATTGGGGA TGCCCGCAGA
    GCCAAAGTCT ATGGCCGTGG CCTGTCAGAA GGCCGGACTT TCGAGATGTC TGACTTCATT
    GTGGACACGA GAGATGCAGG TTACGGAGGC ATCTCCTTGG CGGTGGAAGG CCCTAGCAAG
    GTGGACATCC AGACCGAGGA CCTGGAGGAC GGCACCTGCA AGGTCTCCTA CTTCCCCACT
    GTGCCTGGGG TTTATATCGT CTCCACCAAG TTCGCTGACG AGCACGTGCC TGGGAGCCCG
    TTCACCGTGA AAATCAGCGG GGAGGGAAGA GTCAAGGAGA GCATCACCCG TACCAGCCGA
    GCCCCGTCTG TGGCCACTGT TGGCAGCATC TGTGACCTGA ACCTGAAAAT CCCAGAAATT
    GACAGCAGTG ACATGTCAGC CCACGTCACC AGCCCCTCTG GCCGCGTGAC CGAGGCGGAG
    ATCGTGCCGG TGGGGAAGAA CTCGCACTGC GTCCGGTTCG TGCCCCAGGA GATGGGCGTT
    CACACCGTCA GCGTCAAGTA CCGTGGCCAG CACGTCACTG GCAGCCCCTT CCAGTTCACT
    GTGGGGCCGC TGGGCGAGGG GGGTGCCCAC AAGGTCCGGG CAGGCGGCCC TGGCCTGGAG
    CGAGGAGAAG CCGGAGTTCC GGCGGAGTTC AGCATCTGGA CCCGGGAGGC AGGCGCCGGG
    GGCCTCTCCA TCGCCGTGGA GGGCCCCAGT AAGGCCGAGA TTACATTCGA TGACCATAAA
    AACGGATCTT GTGGTGTGTC TTATATTGCC CAGGAACCTG GTAACTACGA GGTCTCCATC
    AAGTTCAATG ACGAGCATAT TCCCGAAAGT CCCTATGTGG TGCCGGTGAT CGCGCCCTCG
    GACGACGCCC GCCGCCTCAC TGTTCTGAGC CTTCAGGAAT CGGGATTAAA AGTTAATCAG
    CCAGCCTCCT TTGCTATAAG GTTGAACGGG GCAAAAGGCA AGATCGATGC AAAGGTGCAC
    AGCCCCTCTG GAGCCGTGGA GGAATGCCAC GTGTCTGAGC TGGAGCCAGA TAAGTATGCT
    GTTCGCTTCA TCCCCCACGA GAATGGTATC CACACGATTG ACGTCAAGTT CAACGGGAGC
    CACGTGGTTG GAAGTCCCTT CAAAGTGCGC GTCGGGGAGC CTGGACAAGC GGGGAACCCC
    GCCCTGGTGT CGGCCTATGG CGCAGGGCTT GAGGGAGGCT CCACAGGTAT CCAGTCAGAA
    TTTTTCATCA ATACCACCCG AGCAGGGCCA GGGACATTAT CCGTCACCAT CGAAGGGCCG
    TCCAAGGTTA AAATGGATTG CCAGGAAACC CCCGAAGGGT ACAAGGTCAT GTACACCCCC
    ATGGCTCCTG GAAACTACCT GATTGGCGTC AAATATGGCG GGCCCAACCA CATCGTGGGC
    AGCCCGTTCA AGGCCAAGGT GACAGGCCAG CGTCTGGTCA GCCCCGGCTC GGCCAACGAG
    ACCTCCTCCA TCCTGGTGGA GTCAGTGACC AGGTCCTCGA CGGAGACGTG CTACAGCGCC
    ATCCCCAAGG CATCCTCAGA CGCCAGCAAG GTGACCTCCA AGGGCGCAGG GCTCTCGAAG
    GCCTTCCTGG GCCAGAAGAG CTCCTTCCTG GTGGACTGCA GCAAAGCCGG TTCCAACATG
    TTGCTGATCG GGGTCCACGG GCCCACCACC CCCTGCGAAG AAGTCTCCAT GAAGCATGTG
    GGCAACCAGC AGTACAACGT GACCTACGTC GTCAAGGAGC GGGGGGATTA CGTCCTGGCC
    GTGAAGTGGG GAGAGGAGCA CATCCCCGGC AGTCCGTTCC ACGTCACCGT GCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222965.1
    CDS178..7914
    Translation

    Target ORF information:

    RefSeq Version XM_005222908.4
    Organism Bos taurus(cattle)
    Definition Bos taurus filamin B (FLNB), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222908.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA
    GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA
    GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG
    ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT
    GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG
    GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC
    ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA
    TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG
    GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC
    AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT
    GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG
    CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC
    ACTGTCATGG TAACAGAAGA GGCCTATGTC CCAGTGAGCA ACATGAACGG CCTGGGATTT
    AAGCCTTTCG ACTTGGTCAT TCCATTTGCA GTCAGGAAAG GAGAAATCAC CGGAGAGGTC
    CACATGCCTT CCGGGAAGAC GGCCAAGCCC GAGATTGTGG ACAACAAGGA CGGCACAGTC
    ACTGTCAGAT ACGCCCCCAC GGAGGTTGGC CTCCACGAGA TGCACATCAA GTACATGGGC
    AGCCACATTC CTGAGAGCCC CCTCCAGTTC TACGTGAACT ACCCCAACAG TGGGAGCGTG
    TCTGCGTACG GCCCAGGCCT GGTGTACGGA GTGGCCAACA AAACCGCCAC CTTCACCATC
    GTCACCGAAG ACGCCGGAGA AGGTGGCCTG GACTTGGCTA TCGAGGGACC CTCAAAGGCC
    GAAATCAGCT GTATTGACAA CAAAGATGGG ACGTGCACGG TGACTTACCT GCCCACCTTG
    CCGGGCGACT ACAGCATCCT GGTCAAGTAC AATGACAAAC ACATCCCTGG CAGCCCCTTC
    ACAGCCAAGA TCACAGACGA CAGCAGACGG TGCTCTCAGG TGAAGCTGGG CTCAGCCGCC
    GACTTCTTGC TTGACATCAG CGAGACTGAC CTCAGCACCT TGACGGCCAG CATCAAGGCC
    CCGTCCGGTC GTGACGAGCC TTGTCTCCTG AAGAGGCTGC CCAACAATCA CATCGGCATC
    TCCTTCATTC CCCGGGAGGT GGGTGAACAC CTGGTCAGCA TCAAGAAGAA CGGCAACCAC
    GTGGCCAACA GCCCCGTATC CATCATGGTG GTCCAGTCGG AGATTGGGGA TGCCCGCAGA
    GCCAAAGTCT ATGGCCGTGG CCTGTCAGAA GGCCGGACTT TCGAGATGTC TGACTTCATT
    GTGGACACGA GAGATGCAGG TTACGGAGGC ATCTCCTTGG CGGTGGAAGG CCCTAGCAAG
    GTGGACATCC AGACCGAGGA CCTGGAGGAC GGCACCTGCA AGGTCTCCTA CTTCCCCACT
    GTGCCTGGGG TTTATATCGT CTCCACCAAG TTCGCTGACG AGCACGTGCC TGGGAGCCCG
    TTCACCGTGA AAATCAGCGG GGAGGGAAGA GTCAAGGAGA GCATCACCCG TACCAGCCGA
    GCCCCGTCTG TGGCCACTGT TGGCAGCATC TGTGACCTGA ACCTGAAAAT CCCAGAAATT
    GACAGCAGTG ACATGTCAGC CCACGTCACC AGCCCCTCTG GCCGCGTGAC CGAGGCGGAG
    ATCGTGCCGG TGGGGAAGAA CTCGCACTGC GTCCGGTTCG TGCCCCAGGA GATGGGCGTT
    CACACCGTCA GCGTCAAGTA CCGTGGCCAG CACGTCACTG GCAGCCCCTT CCAGTTCACT
    GTGGGGCCGC TGGGCGAGGG GGGTGCCCAC AAGGTCCGGG CAGGCGGCCC TGGCCTGGAG
    CGAGGAGAAG CCGGAGTTCC GGCGGAGTTC AGCATCTGGA CCCGGGAGGC AGGCGCCGGG
    GGCCTCTCCA TCGCCGTGGA GGGCCCCAGT AAGGCCGAGA TTACATTCGA TGACCATAAA
    AACGGATCTT GTGGTGTGTC TTATATTGCC CAGGAACCTG GTAACTACGA GGTCTCCATC
    AAGTTCAATG ACGAGCATAT TCCCGAAAGT CCCTATGTGG TGCCGGTGAT CGCGCCCTCG
    GACGACGCCC GCCGCCTCAC TGTTCTGAGC CTTCAGGAAT CGGGATTAAA AGTTAATCAG
    CCAGCCTCCT TTGCTATAAG GTTGAACGGG GCAAAAGGCA AGATCGATGC AAAGGTGCAC
    AGCCCCTCTG GAGCCGTGGA GGAATGCCAC GTGTCTGAGC TGGAGCCAGA TAAGTATGCT
    GTTCGCTTCA TCCCCCACGA GAATGGTATC CACACGATTG ACGTCAAGTT CAACGGGAGC
    CACGTGGTTG GAAGTCCCTT CAAAGTGCGC GTCGGGGAGC CTGGACAAGC GGGGAACCCC
    GCCCTGGTGT CGGCCTATGG CGCAGGGCTT GAGGGAGGCT CCACAGGTAT CCAGTCAGAA
    TTTTTCATCA ATACCACCCG AGCAGGGCCA GGGACATTAT CCGTCACCAT CGAAGGGCCG
    TCCAAGGTTA AAATGGATTG CCAGGAAACC CCCGAAGGGT ACAAGGTCAT GTACACCCCC
    ATGGCTCCTG GAAACTACCT GATTGGCGTC AAATATGGCG GGCCCAACCA CATCGTGGGC
    AGCCCGTTCA AGGCCAAGGT GACAGGCCAG CGTCTGGTCA GCCCCGGCTC GGCCAACGAG
    ACCTCCTCCA TCCTGGTGGA GTCAGTGACC AGGTCCTCGA CGGAGACGTG CTACAGCGCC
    ATCCCCAAGG CATCCTCAGA CGCCAGCAAG GTGACCTCCA AGGGCGCAGG GCTCTCGAAG
    GCCTTCCTGG GCCAGAAGAG CTCCTTCCTG GTGGACTGCA GCAAAGCCGG TTCCAACATG
    TTGCTGATCG GGGTCCACGG GCCCACCACC CCCTGCGAAG AAGTCTCCAT GAAGCATGTG
    GGCAACCAGC AGTACAACGT GACCTACGTC GTCAAGGAGC GGGGGGATTA CGTCCTGGCC
    GTGAAGTGGG GAGAGGAGCA CATCCCCGGC AGTCCGTTCC ACGTCACCGT GCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40688
    Clone ID Related Accession (Same CDS sequence) NM_001191460.3 , NM_001191460.1
    Accession Version NM_001191460.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product filamin-B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000022.1. On May 30, 2018 this sequence version replaced NM_001191460.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA
    GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG
    AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC
    CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC
    AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC
    TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA
    GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC
    GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG
    ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA
    CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA
    ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT
    TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC
    GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC
    TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC
    GCAGCTGTGA AGGAAGTACC GGTAAATGCA TGTCCCCCTG GATTCAGGCC CTGGGTAACA
    GAAGAGGCCT ATGTCCCAGT GAGCAACATG AACGGCCTGG GATTTAAGCC TTTCGACTTG
    GTCATTCCAT TTGCAGTCAG GAAAGGAGAA ATCACCGGAG AGGTCCACAT GCCTTCCGGG
    AAGACGGCCA AGCCCGAGAT TGTGGACAAC AAGGACGGCA CAGTCACTGT CAGATACGCC
    CCCACGGAGG TTGGCCTCCA CGAGATGCAC ATCAAGTACA TGGGCAGCCA CATTCCTGAG
    AGCCCCCTCC AGTTCTACGT GAACTACCCC AACAGTGGGA GCGTGTCTGC GTACGGCCCA
    GGCCTGGTGT ACGGAGTGGC CAACAAAACC GCCACCTTCA CCATCGTCAC CGAAGACGCC
    GGAGAAGGTG GCCTGGACTT GGCTATCGAG GGACCCTCAA AGGCCGAAAT CAGCTGTATT
    GACAACAAAG ATGGGACGTG CACGGTGACT TACCTGCCCA CCTTGCCGGG CGACTACAGC
    ATCCTGGTCA AGTACAATGA CAAACACATC CCTGGCAGCC CCTTCACAGC CAAGATCACA
    GACGACAGCA GACGGTGCTC TCAGGTGAAG CTGGGCTCAG CCGCCGACTT CTTGCTTGAC
    ATCAGCGAGA CTGACCTCAG CACCTTGACG GCCAGCATCA AGGCCCCGTC CGGTCGTGAC
    GAGCCTTGTC TCCTGAAGAG GCTGCCCAAC AATCACATCG GCATCTCCTT CATTCCCCGG
    GAGGTGGGTG AACACCTGGT CAGCATCAAG AAGAACGGCA ACCACGTGGC CAACAGCCCC
    GTATCCATCA TGGTGGTCCA GTCGGAGATT GGGGATGCCC GCAGAGCCAA AGTCTATGGC
    CGTGGCCTGT CAGAAGGCCG GACTTTCGAG ATGTCTGACT TCATTGTGGA CACGAGAGAT
    GCAGGTTACG GAGGCATCTC CTTGGCGGTG GAAGGCCCTA GCAAGGTGGA CATCCAGACC
    GAGGACCTGG AGGACGGCAC CTGCAAGGTC TCCTACTTCC CCACTGTGCC TGGGGTTTAT
    ATCGTCTCCA CCAAGTTCGC TGACGAGCAC GTGCCTGGGA GCCCGTTCAC CGTGAAAATC
    AGCGGGGAGG GAAGAGTCAA GGAGAGCATC ACCCGTACCA GCCGAGCCCC GTCTGTGGCC
    ACTGTTGGCA GCATCTGTGA CCTGAACCTG AAAATCCCAG AAATTGACAG CAGTGACATG
    TCAGCCCACG TCACCAGCCC CTCTGGCCGC GTGACCGAGG CGGAGATCGT GCCGGTGGGG
    AAGAACTCGC ACTGCGTCCG GTTCGTGCCC CAGGAGATGG GCGTTCACAC CGTCAGCGTC
    AAGTACCGTG GCCAGCACGT CACTGGCAGC CCCTTCCAGT TCACTGTGGG GCCGCTGGGC
    GAGGGGGGTG CCCACAAGGT CCGGGCAGGC GGCCCTGGCC TGGAGCGAGG AGAAGCCGGA
    GTTCCGGCGG AGTTCAGCAT CTGGACCCGG GAGGCAGGCG CCGGGGGCCT CTCCATCGCC
    GTGGAGGGCC CCAGTAAGGC CGAGATTACA TTCGATGACC ATAAAAACGG ATCTTGTGGT
    GTGTCTTATA TTGCCCAGGA ACCTGGTAAC TACGAGGTCT CCATCAAGTT CAATGACGAG
    CATATTCCCG AAAGTCCCTA TGTGGTGCCG GTGATCGCGC CCTCGGACGA CGCCCGCCGC
    CTCACTGTTC TGAGCCTTCA GGAATCGGGA TTAAAAGTTA ATCAGCCAGC CTCCTTTGCT
    ATAAGGTTGA ACGGGGCAAA AGGCAAGATC GATGCAAAGG TGCACAGCCC CTCTGGAGCC
    GTGGAGGAAT GCCACGTGTC TGAGCTGGAG CCAGATAAGT ATGCTGTTCG CTTCATCCCC
    CACGAGAATG GTATCCACAC GATTGACGTC AAGTTCAACG GGAGCCACGT GGTTGGAAGT
    CCCTTCAAAG TGCGCGTCGG GGAGCCTGGA CAAGCGGGGA ACCCCGCCCT GGTGTCGGCC
    TATGGCGCAG GGCTTGAGGG AGGCTCCACA GGTATCCAGT CAGAATTTTT CATCAATACC
    ACCCGAGCAG GGCCAGGGAC ATTATCCGTC ACCATCGAAG GGCCGTCCAA GGTTAAAATG
    GATTGCCAGG AAACCCCCGA AGGGTACAAG GTCATGTACA CCCCCATGGC TCCTGGAAAC
    TACCTGATTG GCGTCAAATA TGGCGGGCCC AACCACATCG TGGGCAGCCC GTTCAAGGCC
    AAGGTGACAG GCCAGCGTCT GGTCAGCCCC GGCTCGGCCA ACGAGACCTC CTCCATCCTG
    GTGGAGTCAG TGACCAGGTC CTCGACGGAG ACGTGCTACA GCGCCATCCC CAAGGCATCC
    TCAGACGCCA GCAAGGTGAC CTCCAAGGGC GCAGGGCTCT CGAAGGCCTT CCTGGGCCAG
    AAGAGCTCCT TCCTGGTGGA CTGCAGCAAA GCCGGTTCCA ACATGTTGCT GATCGGGGTC
    CACGGGCCCA CCACCCCCTG CGAAGAAGTC TCCATGAAGC ATGTGGGCAA CCAGCAGTAC
    AACGTGACCT ACGTCGTCAA GGAGCGGGGG GATTACGTCC TGGCCGTGAA GTGGGGAGAG
    GAGCACATCC CCGGCAGTCC GTTCCACGTC ACCGTGCCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178389.1
    CDS133..8034
    Translation

    Target ORF information:

    RefSeq Version NM_001191460.3
    Organism Bos taurus(cattle)
    Definition Bos taurus filamin B (FLNB), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191460.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA
    GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG
    AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC
    CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC
    AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC
    TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA
    GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC
    GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG
    ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA
    CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA
    ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT
    TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC
    GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC
    TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC
    GCAGCTGTGA AGGAAGTACC GGTAAATGCA TGTCCCCCTG GATTCAGGCC CTGGGTAACA
    GAAGAGGCCT ATGTCCCAGT GAGCAACATG AACGGCCTGG GATTTAAGCC TTTCGACTTG
    GTCATTCCAT TTGCAGTCAG GAAAGGAGAA ATCACCGGAG AGGTCCACAT GCCTTCCGGG
    AAGACGGCCA AGCCCGAGAT TGTGGACAAC AAGGACGGCA CAGTCACTGT CAGATACGCC
    CCCACGGAGG TTGGCCTCCA CGAGATGCAC ATCAAGTACA TGGGCAGCCA CATTCCTGAG
    AGCCCCCTCC AGTTCTACGT GAACTACCCC AACAGTGGGA GCGTGTCTGC GTACGGCCCA
    GGCCTGGTGT ACGGAGTGGC CAACAAAACC GCCACCTTCA CCATCGTCAC CGAAGACGCC
    GGAGAAGGTG GCCTGGACTT GGCTATCGAG GGACCCTCAA AGGCCGAAAT CAGCTGTATT
    GACAACAAAG ATGGGACGTG CACGGTGACT TACCTGCCCA CCTTGCCGGG CGACTACAGC
    ATCCTGGTCA AGTACAATGA CAAACACATC CCTGGCAGCC CCTTCACAGC CAAGATCACA
    GACGACAGCA GACGGTGCTC TCAGGTGAAG CTGGGCTCAG CCGCCGACTT CTTGCTTGAC
    ATCAGCGAGA CTGACCTCAG CACCTTGACG GCCAGCATCA AGGCCCCGTC CGGTCGTGAC
    GAGCCTTGTC TCCTGAAGAG GCTGCCCAAC AATCACATCG GCATCTCCTT CATTCCCCGG
    GAGGTGGGTG AACACCTGGT CAGCATCAAG AAGAACGGCA ACCACGTGGC CAACAGCCCC
    GTATCCATCA TGGTGGTCCA GTCGGAGATT GGGGATGCCC GCAGAGCCAA AGTCTATGGC
    CGTGGCCTGT CAGAAGGCCG GACTTTCGAG ATGTCTGACT TCATTGTGGA CACGAGAGAT
    GCAGGTTACG GAGGCATCTC CTTGGCGGTG GAAGGCCCTA GCAAGGTGGA CATCCAGACC
    GAGGACCTGG AGGACGGCAC CTGCAAGGTC TCCTACTTCC CCACTGTGCC TGGGGTTTAT
    ATCGTCTCCA CCAAGTTCGC TGACGAGCAC GTGCCTGGGA GCCCGTTCAC CGTGAAAATC
    AGCGGGGAGG GAAGAGTCAA GGAGAGCATC ACCCGTACCA GCCGAGCCCC GTCTGTGGCC
    ACTGTTGGCA GCATCTGTGA CCTGAACCTG AAAATCCCAG AAATTGACAG CAGTGACATG
    TCAGCCCACG TCACCAGCCC CTCTGGCCGC GTGACCGAGG CGGAGATCGT GCCGGTGGGG
    AAGAACTCGC ACTGCGTCCG GTTCGTGCCC CAGGAGATGG GCGTTCACAC CGTCAGCGTC
    AAGTACCGTG GCCAGCACGT CACTGGCAGC CCCTTCCAGT TCACTGTGGG GCCGCTGGGC
    GAGGGGGGTG CCCACAAGGT CCGGGCAGGC GGCCCTGGCC TGGAGCGAGG AGAAGCCGGA
    GTTCCGGCGG AGTTCAGCAT CTGGACCCGG GAGGCAGGCG CCGGGGGCCT CTCCATCGCC
    GTGGAGGGCC CCAGTAAGGC CGAGATTACA TTCGATGACC ATAAAAACGG ATCTTGTGGT
    GTGTCTTATA TTGCCCAGGA ACCTGGTAAC TACGAGGTCT CCATCAAGTT CAATGACGAG
    CATATTCCCG AAAGTCCCTA TGTGGTGCCG GTGATCGCGC CCTCGGACGA CGCCCGCCGC
    CTCACTGTTC TGAGCCTTCA GGAATCGGGA TTAAAAGTTA ATCAGCCAGC CTCCTTTGCT
    ATAAGGTTGA ACGGGGCAAA AGGCAAGATC GATGCAAAGG TGCACAGCCC CTCTGGAGCC
    GTGGAGGAAT GCCACGTGTC TGAGCTGGAG CCAGATAAGT ATGCTGTTCG CTTCATCCCC
    CACGAGAATG GTATCCACAC GATTGACGTC AAGTTCAACG GGAGCCACGT GGTTGGAAGT
    CCCTTCAAAG TGCGCGTCGG GGAGCCTGGA CAAGCGGGGA ACCCCGCCCT GGTGTCGGCC
    TATGGCGCAG GGCTTGAGGG AGGCTCCACA GGTATCCAGT CAGAATTTTT CATCAATACC
    ACCCGAGCAG GGCCAGGGAC ATTATCCGTC ACCATCGAAG GGCCGTCCAA GGTTAAAATG
    GATTGCCAGG AAACCCCCGA AGGGTACAAG GTCATGTACA CCCCCATGGC TCCTGGAAAC
    TACCTGATTG GCGTCAAATA TGGCGGGCCC AACCACATCG TGGGCAGCCC GTTCAAGGCC
    AAGGTGACAG GCCAGCGTCT GGTCAGCCCC GGCTCGGCCA ACGAGACCTC CTCCATCCTG
    GTGGAGTCAG TGACCAGGTC CTCGACGGAG ACGTGCTACA GCGCCATCCC CAAGGCATCC
    TCAGACGCCA GCAAGGTGAC CTCCAAGGGC GCAGGGCTCT CGAAGGCCTT CCTGGGCCAG
    AAGAGCTCCT TCCTGGTGGA CTGCAGCAAA GCCGGTTCCA ACATGTTGCT GATCGGGGTC
    CACGGGCCCA CCACCCCCTG CGAAGAAGTC TCCATGAAGC ATGTGGGCAA CCAGCAGTAC
    AACGTGACCT ACGTCGTCAA GGAGCGGGGG GATTACGTCC TGGCCGTGAA GTGGGGAGAG
    GAGCACATCC CCGGCAGTCC GTTCCACGTC ACCGTGCCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40688
    Clone ID Related Accession (Same CDS sequence) NM_001191460.3 , NM_001191460.1
    Accession Version NM_001191460.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product filamin-B
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03009325.1, AAFC03128276.1, AAFC03009324.1 and AAFC03007718.1. On Jul 17, 2010 this sequence version replaced XM_002702763.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA
    GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG
    AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC
    CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC
    AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC
    TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA
    GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC
    GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG
    ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA
    CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA
    ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT
    TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC
    GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC
    TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC
    GCAGCTGTGA AGGAAGTACC GGTAAATGCA TGTCCCCCTG GATTCAGGCC CTGGGTAACA
    GAAGAGGCCT ATGTCCCAGT GAGCAACATG AACGGCCTGG GATTTAAGCC TTTCGACTTG
    GTCATTCCAT TTGCAGTCAG GAAAGGAGAA ATCACCGGAG AGGTCCACAT GCCTTCCGGG
    AAGACGGCCA AGCCCGAGAT TGTGGACAAC AAGGACGGCA CAGTCACTGT CAGATACGCC
    CCCACGGAGG TTGGCCTCCA CGAGATGCAC ATCAAGTACA TGGGCAGCCA CATTCCTGAG
    AGCCCCCTCC AGTTCTACGT GAACTACCCC AACAGTGGGA GCGTGTCTGC GTACGGCCCA
    GGCCTGGTGT ACGGAGTGGC CAACAAAACC GCCACCTTCA CCATCGTCAC CGAAGACGCC
    GGAGAAGGTG GCCTGGACTT GGCTATCGAG GGACCCTCAA AGGCCGAAAT CAGCTGTATT
    GACAACAAAG ATGGGACGTG CACGGTGACT TACCTGCCCA CCTTGCCGGG CGACTACAGC
    ATCCTGGTCA AGTACAATGA CAAACACATC CCTGGCAGCC CCTTCACAGC CAAGATCACA
    GACGACAGCA GACGGTGCTC TCAGGTGAAG CTGGGCTCAG CCGCCGACTT CTTGCTTGAC
    ATCAGCGAGA CTGACCTCAG CACCTTGACG GCCAGCATCA AGGCCCCGTC CGGTCGTGAC
    GAGCCTTGTC TCCTGAAGAG GCTGCCCAAC AATCACATCG GCATCTCCTT CATTCCCCGG
    GAGGTGGGTG AACACCTGGT CAGCATCAAG AAGAACGGCA ACCACGTGGC CAACAGCCCC
    GTATCCATCA TGGTGGTCCA GTCGGAGATT GGGGATGCCC GCAGAGCCAA AGTCTATGGC
    CGTGGCCTGT CAGAAGGCCG GACTTTCGAG ATGTCTGACT TCATTGTGGA CACGAGAGAT
    GCAGGTTACG GAGGCATCTC CTTGGCGGTG GAAGGCCCTA GCAAGGTGGA CATCCAGACC
    GAGGACCTGG AGGACGGCAC CTGCAAGGTC TCCTACTTCC CCACTGTGCC TGGGGTTTAT
    ATCGTCTCCA CCAAGTTCGC TGACGAGCAC GTGCCTGGGA GCCCGTTCAC CGTGAAAATC
    AGCGGGGAGG GAAGAGTCAA GGAGAGCATC ACCCGTACCA GCCGAGCCCC GTCTGTGGCC
    ACTGTTGGCA GCATCTGTGA CCTGAACCTG AAAATCCCAG AAATTGACAG CAGTGACATG
    TCAGCCCACG TCACCAGCCC CTCTGGCCGC GTGACCGAGG CGGAGATCGT GCCGGTGGGG
    AAGAACTCGC ACTGCGTCCG GTTCGTGCCC CAGGAGATGG GCGTTCACAC CGTCAGCGTC
    AAGTACCGTG GCCAGCACGT CACTGGCAGC CCCTTCCAGT TCACTGTGGG GCCGCTGGGC
    GAGGGGGGTG CCCACAAGGT CCGGGCAGGC GGCCCTGGCC TGGAGCGAGG AGAAGCCGGA
    GTTCCGGCGG AGTTCAGCAT CTGGACCCGG GAGGCAGGCG CCGGGGGCCT CTCCATCGCC
    GTGGAGGGCC CCAGTAAGGC CGAGATTACA TTCGATGACC ATAAAAACGG ATCTTGTGGT
    GTGTCTTATA TTGCCCAGGA ACCTGGTAAC TACGAGGTCT CCATCAAGTT CAATGACGAG
    CATATTCCCG AAAGTCCCTA TGTGGTGCCG GTGATCGCGC CCTCGGACGA CGCCCGCCGC
    CTCACTGTTC TGAGCCTTCA GGAATCGGGA TTAAAAGTTA ATCAGCCAGC CTCCTTTGCT
    ATAAGGTTGA ACGGGGCAAA AGGCAAGATC GATGCAAAGG TGCACAGCCC CTCTGGAGCC
    GTGGAGGAAT GCCACGTGTC TGAGCTGGAG CCAGATAAGT ATGCTGTTCG CTTCATCCCC
    CACGAGAATG GTATCCACAC GATTGACGTC AAGTTCAACG GGAGCCACGT GGTTGGAAGT
    CCCTTCAAAG TGCGCGTCGG GGAGCCTGGA CAAGCGGGGA ACCCCGCCCT GGTGTCGGCC
    TATGGCGCAG GGCTTGAGGG AGGCTCCACA GGTATCCAGT CAGAATTTTT CATCAATACC
    ACCCGAGCAG GGCCAGGGAC ATTATCCGTC ACCATCGAAG GGCCGTCCAA GGTTAAAATG
    GATTGCCAGG AAACCCCCGA AGGGTACAAG GTCATGTACA CCCCCATGGC TCCTGGAAAC
    TACCTGATTG GCGTCAAATA TGGCGGGCCC AACCACATCG TGGGCAGCCC GTTCAAGGCC
    AAGGTGACAG GCCAGCGTCT GGTCAGCCCC GGCTCGGCCA ACGAGACCTC CTCCATCCTG
    GTGGAGTCAG TGACCAGGTC CTCGACGGAG ACGTGCTACA GCGCCATCCC CAAGGCATCC
    TCAGACGCCA GCAAGGTGAC CTCCAAGGGC GCAGGGCTCT CGAAGGCCTT CCTGGGCCAG
    AAGAGCTCCT TCCTGGTGGA CTGCAGCAAA GCCGGTTCCA ACATGTTGCT GATCGGGGTC
    CACGGGCCCA CCACCCCCTG CGAAGAAGTC TCCATGAAGC ATGTGGGCAA CCAGCAGTAC
    AACGTGACCT ACGTCGTCAA GGAGCGGGGG GATTACGTCC TGGCCGTGAA GTGGGGAGAG
    GAGCACATCC CCGGCAGTCC GTTCCACGTC ACCGTGCCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178389.1
    CDS133..8034
    Translation

    Target ORF information:

    RefSeq Version NM_001191460.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus filamin B, beta (FLNB), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191460.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA
    GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG
    AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC
    CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC
    AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC
    TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA
    GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC
    GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG
    ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA
    CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA
    ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT
    TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC
    GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC
    TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC
    GCAGCTGTGA AGGAAGTACC GGTAAATGCA TGTCCCCCTG GATTCAGGCC CTGGGTAACA
    GAAGAGGCCT ATGTCCCAGT GAGCAACATG AACGGCCTGG GATTTAAGCC TTTCGACTTG
    GTCATTCCAT TTGCAGTCAG GAAAGGAGAA ATCACCGGAG AGGTCCACAT GCCTTCCGGG
    AAGACGGCCA AGCCCGAGAT TGTGGACAAC AAGGACGGCA CAGTCACTGT CAGATACGCC
    CCCACGGAGG TTGGCCTCCA CGAGATGCAC ATCAAGTACA TGGGCAGCCA CATTCCTGAG
    AGCCCCCTCC AGTTCTACGT GAACTACCCC AACAGTGGGA GCGTGTCTGC GTACGGCCCA
    GGCCTGGTGT ACGGAGTGGC CAACAAAACC GCCACCTTCA CCATCGTCAC CGAAGACGCC
    GGAGAAGGTG GCCTGGACTT GGCTATCGAG GGACCCTCAA AGGCCGAAAT CAGCTGTATT
    GACAACAAAG ATGGGACGTG CACGGTGACT TACCTGCCCA CCTTGCCGGG CGACTACAGC
    ATCCTGGTCA AGTACAATGA CAAACACATC CCTGGCAGCC CCTTCACAGC CAAGATCACA
    GACGACAGCA GACGGTGCTC TCAGGTGAAG CTGGGCTCAG CCGCCGACTT CTTGCTTGAC
    ATCAGCGAGA CTGACCTCAG CACCTTGACG GCCAGCATCA AGGCCCCGTC CGGTCGTGAC
    GAGCCTTGTC TCCTGAAGAG GCTGCCCAAC AATCACATCG GCATCTCCTT CATTCCCCGG
    GAGGTGGGTG AACACCTGGT CAGCATCAAG AAGAACGGCA ACCACGTGGC CAACAGCCCC
    GTATCCATCA TGGTGGTCCA GTCGGAGATT GGGGATGCCC GCAGAGCCAA AGTCTATGGC
    CGTGGCCTGT CAGAAGGCCG GACTTTCGAG ATGTCTGACT TCATTGTGGA CACGAGAGAT
    GCAGGTTACG GAGGCATCTC CTTGGCGGTG GAAGGCCCTA GCAAGGTGGA CATCCAGACC
    GAGGACCTGG AGGACGGCAC CTGCAAGGTC TCCTACTTCC CCACTGTGCC TGGGGTTTAT
    ATCGTCTCCA CCAAGTTCGC TGACGAGCAC GTGCCTGGGA GCCCGTTCAC CGTGAAAATC
    AGCGGGGAGG GAAGAGTCAA GGAGAGCATC ACCCGTACCA GCCGAGCCCC GTCTGTGGCC
    ACTGTTGGCA GCATCTGTGA CCTGAACCTG AAAATCCCAG AAATTGACAG CAGTGACATG
    TCAGCCCACG TCACCAGCCC CTCTGGCCGC GTGACCGAGG CGGAGATCGT GCCGGTGGGG
    AAGAACTCGC ACTGCGTCCG GTTCGTGCCC CAGGAGATGG GCGTTCACAC CGTCAGCGTC
    AAGTACCGTG GCCAGCACGT CACTGGCAGC CCCTTCCAGT TCACTGTGGG GCCGCTGGGC
    GAGGGGGGTG CCCACAAGGT CCGGGCAGGC GGCCCTGGCC TGGAGCGAGG AGAAGCCGGA
    GTTCCGGCGG AGTTCAGCAT CTGGACCCGG GAGGCAGGCG CCGGGGGCCT CTCCATCGCC
    GTGGAGGGCC CCAGTAAGGC CGAGATTACA TTCGATGACC ATAAAAACGG ATCTTGTGGT
    GTGTCTTATA TTGCCCAGGA ACCTGGTAAC TACGAGGTCT CCATCAAGTT CAATGACGAG
    CATATTCCCG AAAGTCCCTA TGTGGTGCCG GTGATCGCGC CCTCGGACGA CGCCCGCCGC
    CTCACTGTTC TGAGCCTTCA GGAATCGGGA TTAAAAGTTA ATCAGCCAGC CTCCTTTGCT
    ATAAGGTTGA ACGGGGCAAA AGGCAAGATC GATGCAAAGG TGCACAGCCC CTCTGGAGCC
    GTGGAGGAAT GCCACGTGTC TGAGCTGGAG CCAGATAAGT ATGCTGTTCG CTTCATCCCC
    CACGAGAATG GTATCCACAC GATTGACGTC AAGTTCAACG GGAGCCACGT GGTTGGAAGT
    CCCTTCAAAG TGCGCGTCGG GGAGCCTGGA CAAGCGGGGA ACCCCGCCCT GGTGTCGGCC
    TATGGCGCAG GGCTTGAGGG AGGCTCCACA GGTATCCAGT CAGAATTTTT CATCAATACC
    ACCCGAGCAG GGCCAGGGAC ATTATCCGTC ACCATCGAAG GGCCGTCCAA GGTTAAAATG
    GATTGCCAGG AAACCCCCGA AGGGTACAAG GTCATGTACA CCCCCATGGC TCCTGGAAAC
    TACCTGATTG GCGTCAAATA TGGCGGGCCC AACCACATCG TGGGCAGCCC GTTCAAGGCC
    AAGGTGACAG GCCAGCGTCT GGTCAGCCCC GGCTCGGCCA ACGAGACCTC CTCCATCCTG
    GTGGAGTCAG TGACCAGGTC CTCGACGGAG ACGTGCTACA GCGCCATCCC CAAGGCATCC
    TCAGACGCCA GCAAGGTGAC CTCCAAGGGC GCAGGGCTCT CGAAGGCCTT CCTGGGCCAG
    AAGAGCTCCT TCCTGGTGGA CTGCAGCAAA GCCGGTTCCA ACATGTTGCT GATCGGGGTC
    CACGGGCCCA CCACCCCCTG CGAAGAAGTC TCCATGAAGC ATGTGGGCAA CCAGCAGTAC
    AACGTGACCT ACGTCGTCAA GGAGCGGGGG GATTACGTCC TGGCCGTGAA GTGGGGAGAG
    GAGCACATCC CCGGCAGTCC GTTCCACGTC ACCGTGCCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31625
    Clone ID Related Accession (Same CDS sequence) XM_005222904.3
    Accession Version XM_005222904.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product filamin-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222904.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA
    GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG
    AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC
    CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC
    AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC
    TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA
    GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC
    GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG
    ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA
    CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA
    ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT
    TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC
    GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC
    TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC
    GCAGCTGTGA AGGAAGTACC GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC
    GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC
    ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG
    GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC
    AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC
    AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC
    ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA
    CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC
    CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT
    GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG
    GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC
    AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT
    CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG
    AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG
    GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG
    TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA
    GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC
    TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG
    CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC
    CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA
    ATCCCAGAAA TTGACAGCAG TGACATGTCG GCCCACGTCA CCAGCCCCTC TGGCCGCGTG
    ACCGAGGCAG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG
    GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC
    TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC
    CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG
    GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC
    GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC
    GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG
    ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA
    AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT
    GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA
    GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG
    TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA
    GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT
    ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC
    ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC
    ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC
    CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC
    TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG
    TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA
    GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC
    GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC
    ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT
    TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC
    GTGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222961.1
    CDS177..8045
    Translation

    Target ORF information:

    RefSeq Version XM_005222904.3
    Organism Bos taurus(cattle)
    Definition Bos taurus filamin B (FLNB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222904.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA
    GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG
    AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC
    CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC
    AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC
    TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA
    GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC
    GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG
    ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA
    CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA
    ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT
    TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC
    GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC
    TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC
    GCAGCTGTGA AGGAAGTACC GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC
    GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC
    ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG
    GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC
    AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC
    AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC
    ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA
    CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC
    CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT
    GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG
    GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC
    AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT
    CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG
    AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG
    GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG
    TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA
    GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC
    TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG
    CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC
    CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA
    ATCCCAGAAA TTGACAGCAG TGACATGTCG GCCCACGTCA CCAGCCCCTC TGGCCGCGTG
    ACCGAGGCAG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG
    GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC
    TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC
    CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG
    GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC
    GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC
    GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG
    ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA
    AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT
    GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA
    GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG
    TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA
    GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT
    ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC
    ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC
    ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC
    CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC
    TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG
    TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA
    GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC
    GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC
    ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT
    TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC
    GTGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31626
    Clone ID Related Accession (Same CDS sequence) XM_005222905.3
    Accession Version XM_005222905.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product filamin-B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222905.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
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    1321
    1381
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    1501
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    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA
    GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG
    AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC
    CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC
    AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC
    TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA
    GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC
    GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG
    ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA
    CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA
    ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT
    TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC
    GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC
    TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGTAACAGA AGAGGCCTAT
    GTCCCAGTGA GCAACATGAA CGGCCTGGGA TTTAAGCCTT TCGACTTGGT CATTCCATTT
    GCAGTCAGGA AAGGAGAAAT CACCGGAGAG GTCCACATGC CTTCCGGGAA GACGGCCAAG
    CCCGAGATTG TGGACAACAA GGACGGCACA GTCACTGTCA GATACGCCCC CACGGAGGTT
    GGCCTCCACG AGATGCACAT CAAGTACATG GGCAGCCACA TTCCTGAGAG CCCCCTCCAG
    TTCTACGTGA ACTACCCCAA CAGTGGGAGC GTGTCTGCGT ACGGCCCAGG CCTGGTGTAC
    GGAGTGGCCA ACAAAACCGC CACCTTCACC ATCGTCACCG AAGACGCCGG AGAAGGTGGC
    CTGGACTTGG CTATCGAGGG ACCCTCAAAG GCCGAAATCA GCTGTATTGA CAACAAAGAT
    GGGACGTGCA CGGTGACTTA CCTGCCCACC TTGCCGGGCG ACTACAGCAT CCTGGTCAAG
    TACAATGACA AACACATCCC TGGCAGCCCC TTCACAGCCA AGATCACAGA CGACAGCAGA
    CGGTGCTCTC AGGTGAAGCT GGGCTCAGCC GCCGACTTCT TGCTTGACAT CAGCGAGACT
    GACCTCAGCA CCTTGACGGC CAGCATCAAG GCCCCGTCCG GTCGTGACGA GCCTTGTCTC
    CTGAAGAGGC TGCCCAACAA TCACATCGGC ATCTCCTTCA TTCCCCGGGA GGTGGGTGAA
    CACCTGGTCA GCATCAAGAA GAACGGCAAC CACGTGGCCA ACAGCCCCGT ATCCATCATG
    GTGGTCCAGT CGGAGATTGG GGATGCCCGC AGAGCCAAAG TCTATGGCCG TGGCCTGTCA
    GAAGGCCGGA CTTTCGAGAT GTCTGACTTC ATTGTGGACA CGAGAGATGC AGGTTACGGA
    GGCATCTCCT TGGCGGTGGA AGGCCCTAGC AAGGTGGACA TCCAGACCGA GGACCTGGAG
    GACGGCACCT GCAAGGTCTC CTACTTCCCC ACTGTGCCTG GGGTTTATAT CGTCTCCACC
    AAGTTCGCTG ACGAGCACGT GCCTGGGAGC CCGTTCACCG TGAAAATCAG CGGGGAGGGA
    AGAGTCAAGG AGAGCATCAC CCGTACCAGC CGAGCCCCGT CTGTGGCCAC TGTTGGCAGC
    ATCTGTGACC TGAACCTGAA AATCCCAGAA ATTGACAGCA GTGACATGTC GGCCCACGTC
    ACCAGCCCCT CTGGCCGCGT GACCGAGGCA GAGATCGTGC CGGTGGGGAA GAACTCGCAC
    TGCGTCCGGT TCGTGCCCCA GGAGATGGGC GTTCACACCG TCAGCGTCAA GTACCGTGGC
    CAGCACGTCA CTGGCAGCCC CTTCCAGTTC ACTGTGGGGC CGCTGGGCGA GGGGGGTGCC
    CACAAGGTCC GGGCAGGCGG CCCTGGCCTG GAGCGAGGAG AAGCCGGAGT TCCGGCGGAG
    TTCAGCATCT GGACCCGGGA GGCAGGCGCC GGGGGCCTCT CCATCGCCGT GGAGGGCCCC
    AGTAAGGCCG AGATTACATT CGATGACCAT AAAAACGGAT CTTGTGGTGT GTCTTATATT
    GCCCAGGAAC CTGGTAACTA CGAGGTCTCC ATCAAGTTCA ATGACGAGCA TATTCCCGAA
    AGTCCCTATG TGGTGCCGGT GATCGCGCCC TCGGACGACG CCCGCCGCCT CACTGTTCTG
    AGCCTTCAGG AATCGGGATT AAAAGTTAAT CAGCCAGCCT CCTTTGCTAT AAGGTTGAAC
    GGGGCAAAAG GCAAGATCGA TGCAAAGGTG CACAGCCCCT CTGGAGCCGT GGAGGAATGC
    CACGTGTCTG AGCTGGAGCC AGATAAGTAT GCTGTTCGCT TCATCCCCCA CGAGAATGGT
    ATCCACACGA TTGACGTCAA GTTCAACGGG AGCCACGTGG TTGGAAGTCC CTTCAAAGTG
    CGCGTCGGGG AGCCTGGACA AGCGGGGAAC CCCGCCCTGG TGTCGGCCTA TGGCGCAGGG
    CTTGAGGGAG GCTCCACAGG TATCCAGTCA GAATTTTTCA TCAATACCAC CCGAGCAGGG
    CCAGGGACAT TATCCGTCAC CATCGAAGGG CCGTCCAAGG TTAAAATGGA TTGCCAGGAA
    ACCCCCGAAG GGTACAAGGT CATGTACACC CCCATGGCTC CTGGAAACTA CCTGATTGGC
    GTCAAATATG GCGGGCCCAA CCACATCGTG GGCAGCCCGT TCAAGGCCAA GGTGACAGGC
    CAGCGTCTGG TCAGCCCCGG CTCGGCCAAC GAGACCTCCT CCATCCTGGT GGAGTCAGTG
    ACCAGGTCCT CGACGGAGAC GTGCTACAGC GCCATCCCCA AGGCATCCTC AGACGCCAGC
    AAGGTGACCT CCAAGGGCGC AGGGCTCTCG AAGGCCTTCC TGGGCCAGAA GAGCTCCTTC
    CTGGTGGACT GCAGCAAAGC CGGTTCCAAC ATGTTGCTGA TCGGGGTCCA CGGGCCCACC
    ACCCCCTGCG AAGAAGTCTC CATGAAGCAT GTGGGCAACC AGCAGTACAA CGTGACCTAC
    GTCGTCAAGG AGCGGGGGGA TTACGTCCTG GCCGTGAAGT GGGGAGAGGA GCACATCCCC
    GGCAGTCCGT TCCACGTCAC CGTGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222962.1
    CDS177..8006
    Translation

    Target ORF information:

    RefSeq Version XM_005222905.3
    Organism Bos taurus(cattle)
    Definition Bos taurus filamin B (FLNB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222905.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA
    GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG
    AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC
    CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC
    AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC
    TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA
    GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC
    GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG
    ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA
    CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA
    ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT
    TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC
    GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC
    TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGTAACAGA AGAGGCCTAT
    GTCCCAGTGA GCAACATGAA CGGCCTGGGA TTTAAGCCTT TCGACTTGGT CATTCCATTT
    GCAGTCAGGA AAGGAGAAAT CACCGGAGAG GTCCACATGC CTTCCGGGAA GACGGCCAAG
    CCCGAGATTG TGGACAACAA GGACGGCACA GTCACTGTCA GATACGCCCC CACGGAGGTT
    GGCCTCCACG AGATGCACAT CAAGTACATG GGCAGCCACA TTCCTGAGAG CCCCCTCCAG
    TTCTACGTGA ACTACCCCAA CAGTGGGAGC GTGTCTGCGT ACGGCCCAGG CCTGGTGTAC
    GGAGTGGCCA ACAAAACCGC CACCTTCACC ATCGTCACCG AAGACGCCGG AGAAGGTGGC
    CTGGACTTGG CTATCGAGGG ACCCTCAAAG GCCGAAATCA GCTGTATTGA CAACAAAGAT
    GGGACGTGCA CGGTGACTTA CCTGCCCACC TTGCCGGGCG ACTACAGCAT CCTGGTCAAG
    TACAATGACA AACACATCCC TGGCAGCCCC TTCACAGCCA AGATCACAGA CGACAGCAGA
    CGGTGCTCTC AGGTGAAGCT GGGCTCAGCC GCCGACTTCT TGCTTGACAT CAGCGAGACT
    GACCTCAGCA CCTTGACGGC CAGCATCAAG GCCCCGTCCG GTCGTGACGA GCCTTGTCTC
    CTGAAGAGGC TGCCCAACAA TCACATCGGC ATCTCCTTCA TTCCCCGGGA GGTGGGTGAA
    CACCTGGTCA GCATCAAGAA GAACGGCAAC CACGTGGCCA ACAGCCCCGT ATCCATCATG
    GTGGTCCAGT CGGAGATTGG GGATGCCCGC AGAGCCAAAG TCTATGGCCG TGGCCTGTCA
    GAAGGCCGGA CTTTCGAGAT GTCTGACTTC ATTGTGGACA CGAGAGATGC AGGTTACGGA
    GGCATCTCCT TGGCGGTGGA AGGCCCTAGC AAGGTGGACA TCCAGACCGA GGACCTGGAG
    GACGGCACCT GCAAGGTCTC CTACTTCCCC ACTGTGCCTG GGGTTTATAT CGTCTCCACC
    AAGTTCGCTG ACGAGCACGT GCCTGGGAGC CCGTTCACCG TGAAAATCAG CGGGGAGGGA
    AGAGTCAAGG AGAGCATCAC CCGTACCAGC CGAGCCCCGT CTGTGGCCAC TGTTGGCAGC
    ATCTGTGACC TGAACCTGAA AATCCCAGAA ATTGACAGCA GTGACATGTC GGCCCACGTC
    ACCAGCCCCT CTGGCCGCGT GACCGAGGCA GAGATCGTGC CGGTGGGGAA GAACTCGCAC
    TGCGTCCGGT TCGTGCCCCA GGAGATGGGC GTTCACACCG TCAGCGTCAA GTACCGTGGC
    CAGCACGTCA CTGGCAGCCC CTTCCAGTTC ACTGTGGGGC CGCTGGGCGA GGGGGGTGCC
    CACAAGGTCC GGGCAGGCGG CCCTGGCCTG GAGCGAGGAG AAGCCGGAGT TCCGGCGGAG
    TTCAGCATCT GGACCCGGGA GGCAGGCGCC GGGGGCCTCT CCATCGCCGT GGAGGGCCCC
    AGTAAGGCCG AGATTACATT CGATGACCAT AAAAACGGAT CTTGTGGTGT GTCTTATATT
    GCCCAGGAAC CTGGTAACTA CGAGGTCTCC ATCAAGTTCA ATGACGAGCA TATTCCCGAA
    AGTCCCTATG TGGTGCCGGT GATCGCGCCC TCGGACGACG CCCGCCGCCT CACTGTTCTG
    AGCCTTCAGG AATCGGGATT AAAAGTTAAT CAGCCAGCCT CCTTTGCTAT AAGGTTGAAC
    GGGGCAAAAG GCAAGATCGA TGCAAAGGTG CACAGCCCCT CTGGAGCCGT GGAGGAATGC
    CACGTGTCTG AGCTGGAGCC AGATAAGTAT GCTGTTCGCT TCATCCCCCA CGAGAATGGT
    ATCCACACGA TTGACGTCAA GTTCAACGGG AGCCACGTGG TTGGAAGTCC CTTCAAAGTG
    CGCGTCGGGG AGCCTGGACA AGCGGGGAAC CCCGCCCTGG TGTCGGCCTA TGGCGCAGGG
    CTTGAGGGAG GCTCCACAGG TATCCAGTCA GAATTTTTCA TCAATACCAC CCGAGCAGGG
    CCAGGGACAT TATCCGTCAC CATCGAAGGG CCGTCCAAGG TTAAAATGGA TTGCCAGGAA
    ACCCCCGAAG GGTACAAGGT CATGTACACC CCCATGGCTC CTGGAAACTA CCTGATTGGC
    GTCAAATATG GCGGGCCCAA CCACATCGTG GGCAGCCCGT TCAAGGCCAA GGTGACAGGC
    CAGCGTCTGG TCAGCCCCGG CTCGGCCAAC GAGACCTCCT CCATCCTGGT GGAGTCAGTG
    ACCAGGTCCT CGACGGAGAC GTGCTACAGC GCCATCCCCA AGGCATCCTC AGACGCCAGC
    AAGGTGACCT CCAAGGGCGC AGGGCTCTCG AAGGCCTTCC TGGGCCAGAA GAGCTCCTTC
    CTGGTGGACT GCAGCAAAGC CGGTTCCAAC ATGTTGCTGA TCGGGGTCCA CGGGCCCACC
    ACCCCCTGCG AAGAAGTCTC CATGAAGCAT GTGGGCAACC AGCAGTACAA CGTGACCTAC
    GTCGTCAAGG AGCGGGGGGA TTACGTCCTG GCCGTGAAGT GGGGAGAGGA GCACATCCCC
    GGCAGTCCGT TCCACGTCAC CGTGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31627
    Clone ID Related Accession (Same CDS sequence) XM_005222906.3
    Accession Version XM_005222906.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product filamin-B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222906.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA
    GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA
    GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG
    ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT
    GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG
    GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC
    ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA
    TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG
    GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC
    AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT
    GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG
    CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC
    ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AAATGCATGT
    CCCCCTGGAT TCAGGCCCTG GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC
    GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC
    ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG
    GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC
    AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC
    AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC
    ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA
    CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC
    CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT
    GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG
    GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC
    AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT
    CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG
    AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG
    GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG
    TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA
    GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC
    TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG
    CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC
    CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA
    ATCCCAGAAA TTGACAGCAG TGACATGTCG GCCCACGTCA CCAGCCCCTC TGGCCGCGTG
    ACCGAGGCAG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG
    GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC
    TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC
    CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG
    GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC
    GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC
    GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG
    ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA
    AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT
    GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA
    GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG
    TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA
    GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT
    ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC
    ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC
    ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC
    CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC
    TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG
    TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA
    GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC
    GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC
    ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT
    TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC
    GTGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222963.1
    CDS177..7985
    Translation

    Target ORF information:

    RefSeq Version XM_005222906.3
    Organism Bos taurus(cattle)
    Definition Bos taurus filamin B (FLNB), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222906.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA
    GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA
    GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG
    ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT
    GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG
    GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC
    ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA
    TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG
    GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC
    AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT
    GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG
    CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC
    ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AAATGCATGT
    CCCCCTGGAT TCAGGCCCTG GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC
    GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC
    ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG
    GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC
    AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC
    AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC
    ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA
    CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC
    CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT
    GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG
    GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC
    AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT
    CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG
    AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG
    GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG
    TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA
    GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC
    TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG
    CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC
    CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA
    ATCCCAGAAA TTGACAGCAG TGACATGTCG GCCCACGTCA CCAGCCCCTC TGGCCGCGTG
    ACCGAGGCAG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG
    GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC
    TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC
    CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG
    GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC
    GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC
    GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG
    ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA
    AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT
    GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA
    GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG
    TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA
    GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT
    ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC
    ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC
    ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC
    CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC
    TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG
    TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA
    GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC
    GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC
    ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT
    TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC
    GTGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31628
    Clone ID Related Accession (Same CDS sequence) XM_005222907.3
    Accession Version XM_005222907.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product filamin-B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222907.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA
    GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA
    GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG
    ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT
    GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG
    GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC
    ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA
    TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG
    GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC
    AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT
    GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG
    CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC
    ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AACAGAAGAG
    GCCTATGTCC CAGTGAGCAA CATGAACGGC CTGGGATTTA AGCCTTTCGA CTTGGTCATT
    CCATTTGCAG TCAGGAAAGG AGAAATCACC GGAGAGGTCC ACATGCCTTC CGGGAAGACG
    GCCAAGCCCG AGATTGTGGA CAACAAGGAC GGCACAGTCA CTGTCAGATA CGCCCCCACG
    GAGGTTGGCC TCCACGAGAT GCACATCAAG TACATGGGCA GCCACATTCC TGAGAGCCCC
    CTCCAGTTCT ACGTGAACTA CCCCAACAGT GGGAGCGTGT CTGCGTACGG CCCAGGCCTG
    GTGTACGGAG TGGCCAACAA AACCGCCACC TTCACCATCG TCACCGAAGA CGCCGGAGAA
    GGTGGCCTGG ACTTGGCTAT CGAGGGACCC TCAAAGGCCG AAATCAGCTG TATTGACAAC
    AAAGATGGGA CGTGCACGGT GACTTACCTG CCCACCTTGC CGGGCGACTA CAGCATCCTG
    GTCAAGTACA ATGACAAACA CATCCCTGGC AGCCCCTTCA CAGCCAAGAT CACAGACGAC
    AGCAGACGGT GCTCTCAGGT GAAGCTGGGC TCAGCCGCCG ACTTCTTGCT TGACATCAGC
    GAGACTGACC TCAGCACCTT GACGGCCAGC ATCAAGGCCC CGTCCGGTCG TGACGAGCCT
    TGTCTCCTGA AGAGGCTGCC CAACAATCAC ATCGGCATCT CCTTCATTCC CCGGGAGGTG
    GGTGAACACC TGGTCAGCAT CAAGAAGAAC GGCAACCACG TGGCCAACAG CCCCGTATCC
    ATCATGGTGG TCCAGTCGGA GATTGGGGAT GCCCGCAGAG CCAAAGTCTA TGGCCGTGGC
    CTGTCAGAAG GCCGGACTTT CGAGATGTCT GACTTCATTG TGGACACGAG AGATGCAGGT
    TACGGAGGCA TCTCCTTGGC GGTGGAAGGC CCTAGCAAGG TGGACATCCA GACCGAGGAC
    CTGGAGGACG GCACCTGCAA GGTCTCCTAC TTCCCCACTG TGCCTGGGGT TTATATCGTC
    TCCACCAAGT TCGCTGACGA GCACGTGCCT GGGAGCCCGT TCACCGTGAA AATCAGCGGG
    GAGGGAAGAG TCAAGGAGAG CATCACCCGT ACCAGCCGAG CCCCGTCTGT GGCCACTGTT
    GGCAGCATCT GTGACCTGAA CCTGAAAATC CCAGAAATTG ACAGCAGTGA CATGTCGGCC
    CACGTCACCA GCCCCTCTGG CCGCGTGACC GAGGCAGAGA TCGTGCCGGT GGGGAAGAAC
    TCGCACTGCG TCCGGTTCGT GCCCCAGGAG ATGGGCGTTC ACACCGTCAG CGTCAAGTAC
    CGTGGCCAGC ACGTCACTGG CAGCCCCTTC CAGTTCACTG TGGGGCCGCT GGGCGAGGGG
    GGTGCCCACA AGGTCCGGGC AGGCGGCCCT GGCCTGGAGC GAGGAGAAGC CGGAGTTCCG
    GCGGAGTTCA GCATCTGGAC CCGGGAGGCA GGCGCCGGGG GCCTCTCCAT CGCCGTGGAG
    GGCCCCAGTA AGGCCGAGAT TACATTCGAT GACCATAAAA ACGGATCTTG TGGTGTGTCT
    TATATTGCCC AGGAACCTGG TAACTACGAG GTCTCCATCA AGTTCAATGA CGAGCATATT
    CCCGAAAGTC CCTATGTGGT GCCGGTGATC GCGCCCTCGG ACGACGCCCG CCGCCTCACT
    GTTCTGAGCC TTCAGGAATC GGGATTAAAA GTTAATCAGC CAGCCTCCTT TGCTATAAGG
    TTGAACGGGG CAAAAGGCAA GATCGATGCA AAGGTGCACA GCCCCTCTGG AGCCGTGGAG
    GAATGCCACG TGTCTGAGCT GGAGCCAGAT AAGTATGCTG TTCGCTTCAT CCCCCACGAG
    AATGGTATCC ACACGATTGA CGTCAAGTTC AACGGGAGCC ACGTGGTTGG AAGTCCCTTC
    AAAGTGCGCG TCGGGGAGCC TGGACAAGCG GGGAACCCCG CCCTGGTGTC GGCCTATGGC
    GCAGGGCTTG AGGGAGGCTC CACAGGTATC CAGTCAGAAT TTTTCATCAA TACCACCCGA
    GCAGGGCCAG GGACATTATC CGTCACCATC GAAGGGCCGT CCAAGGTTAA AATGGATTGC
    CAGGAAACCC CCGAAGGGTA CAAGGTCATG TACACCCCCA TGGCTCCTGG AAACTACCTG
    ATTGGCGTCA AATATGGCGG GCCCAACCAC ATCGTGGGCA GCCCGTTCAA GGCCAAGGTG
    ACAGGCCAGC GTCTGGTCAG CCCCGGCTCG GCCAACGAGA CCTCCTCCAT CCTGGTGGAG
    TCAGTGACCA GGTCCTCGAC GGAGACGTGC TACAGCGCCA TCCCCAAGGC ATCCTCAGAC
    GCCAGCAAGG TGACCTCCAA GGGCGCAGGG CTCTCGAAGG CCTTCCTGGG CCAGAAGAGC
    TCCTTCCTGG TGGACTGCAG CAAAGCCGGT TCCAACATGT TGCTGATCGG GGTCCACGGG
    CCCACCACCC CCTGCGAAGA AGTCTCCATG AAGCATGTGG GCAACCAGCA GTACAACGTG
    ACCTACGTCG TCAAGGAGCG GGGGGATTAC GTCCTGGCCG TGAAGTGGGG AGAGGAGCAC
    ATCCCCGGCA GTCCGTTCCA CGTCACCGTG CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222964.1
    CDS177..7952
    Translation

    Target ORF information:

    RefSeq Version XM_005222907.3
    Organism Bos taurus(cattle)
    Definition Bos taurus filamin B (FLNB), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222907.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA
    GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA
    GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG
    ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT
    GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG
    GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC
    ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA
    TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG
    GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC
    AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT
    GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG
    CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC
    ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AACAGAAGAG
    GCCTATGTCC CAGTGAGCAA CATGAACGGC CTGGGATTTA AGCCTTTCGA CTTGGTCATT
    CCATTTGCAG TCAGGAAAGG AGAAATCACC GGAGAGGTCC ACATGCCTTC CGGGAAGACG
    GCCAAGCCCG AGATTGTGGA CAACAAGGAC GGCACAGTCA CTGTCAGATA CGCCCCCACG
    GAGGTTGGCC TCCACGAGAT GCACATCAAG TACATGGGCA GCCACATTCC TGAGAGCCCC
    CTCCAGTTCT ACGTGAACTA CCCCAACAGT GGGAGCGTGT CTGCGTACGG CCCAGGCCTG
    GTGTACGGAG TGGCCAACAA AACCGCCACC TTCACCATCG TCACCGAAGA CGCCGGAGAA
    GGTGGCCTGG ACTTGGCTAT CGAGGGACCC TCAAAGGCCG AAATCAGCTG TATTGACAAC
    AAAGATGGGA CGTGCACGGT GACTTACCTG CCCACCTTGC CGGGCGACTA CAGCATCCTG
    GTCAAGTACA ATGACAAACA CATCCCTGGC AGCCCCTTCA CAGCCAAGAT CACAGACGAC
    AGCAGACGGT GCTCTCAGGT GAAGCTGGGC TCAGCCGCCG ACTTCTTGCT TGACATCAGC
    GAGACTGACC TCAGCACCTT GACGGCCAGC ATCAAGGCCC CGTCCGGTCG TGACGAGCCT
    TGTCTCCTGA AGAGGCTGCC CAACAATCAC ATCGGCATCT CCTTCATTCC CCGGGAGGTG
    GGTGAACACC TGGTCAGCAT CAAGAAGAAC GGCAACCACG TGGCCAACAG CCCCGTATCC
    ATCATGGTGG TCCAGTCGGA GATTGGGGAT GCCCGCAGAG CCAAAGTCTA TGGCCGTGGC
    CTGTCAGAAG GCCGGACTTT CGAGATGTCT GACTTCATTG TGGACACGAG AGATGCAGGT
    TACGGAGGCA TCTCCTTGGC GGTGGAAGGC CCTAGCAAGG TGGACATCCA GACCGAGGAC
    CTGGAGGACG GCACCTGCAA GGTCTCCTAC TTCCCCACTG TGCCTGGGGT TTATATCGTC
    TCCACCAAGT TCGCTGACGA GCACGTGCCT GGGAGCCCGT TCACCGTGAA AATCAGCGGG
    GAGGGAAGAG TCAAGGAGAG CATCACCCGT ACCAGCCGAG CCCCGTCTGT GGCCACTGTT
    GGCAGCATCT GTGACCTGAA CCTGAAAATC CCAGAAATTG ACAGCAGTGA CATGTCGGCC
    CACGTCACCA GCCCCTCTGG CCGCGTGACC GAGGCAGAGA TCGTGCCGGT GGGGAAGAAC
    TCGCACTGCG TCCGGTTCGT GCCCCAGGAG ATGGGCGTTC ACACCGTCAG CGTCAAGTAC
    CGTGGCCAGC ACGTCACTGG CAGCCCCTTC CAGTTCACTG TGGGGCCGCT GGGCGAGGGG
    GGTGCCCACA AGGTCCGGGC AGGCGGCCCT GGCCTGGAGC GAGGAGAAGC CGGAGTTCCG
    GCGGAGTTCA GCATCTGGAC CCGGGAGGCA GGCGCCGGGG GCCTCTCCAT CGCCGTGGAG
    GGCCCCAGTA AGGCCGAGAT TACATTCGAT GACCATAAAA ACGGATCTTG TGGTGTGTCT
    TATATTGCCC AGGAACCTGG TAACTACGAG GTCTCCATCA AGTTCAATGA CGAGCATATT
    CCCGAAAGTC CCTATGTGGT GCCGGTGATC GCGCCCTCGG ACGACGCCCG CCGCCTCACT
    GTTCTGAGCC TTCAGGAATC GGGATTAAAA GTTAATCAGC CAGCCTCCTT TGCTATAAGG
    TTGAACGGGG CAAAAGGCAA GATCGATGCA AAGGTGCACA GCCCCTCTGG AGCCGTGGAG
    GAATGCCACG TGTCTGAGCT GGAGCCAGAT AAGTATGCTG TTCGCTTCAT CCCCCACGAG
    AATGGTATCC ACACGATTGA CGTCAAGTTC AACGGGAGCC ACGTGGTTGG AAGTCCCTTC
    AAAGTGCGCG TCGGGGAGCC TGGACAAGCG GGGAACCCCG CCCTGGTGTC GGCCTATGGC
    GCAGGGCTTG AGGGAGGCTC CACAGGTATC CAGTCAGAAT TTTTCATCAA TACCACCCGA
    GCAGGGCCAG GGACATTATC CGTCACCATC GAAGGGCCGT CCAAGGTTAA AATGGATTGC
    CAGGAAACCC CCGAAGGGTA CAAGGTCATG TACACCCCCA TGGCTCCTGG AAACTACCTG
    ATTGGCGTCA AATATGGCGG GCCCAACCAC ATCGTGGGCA GCCCGTTCAA GGCCAAGGTG
    ACAGGCCAGC GTCTGGTCAG CCCCGGCTCG GCCAACGAGA CCTCCTCCAT CCTGGTGGAG
    TCAGTGACCA GGTCCTCGAC GGAGACGTGC TACAGCGCCA TCCCCAAGGC ATCCTCAGAC
    GCCAGCAAGG TGACCTCCAA GGGCGCAGGG CTCTCGAAGG CCTTCCTGGG CCAGAAGAGC
    TCCTTCCTGG TGGACTGCAG CAAAGCCGGT TCCAACATGT TGCTGATCGG GGTCCACGGG
    CCCACCACCC CCTGCGAAGA AGTCTCCATG AAGCATGTGG GCAACCAGCA GTACAACGTG
    ACCTACGTCG TCAAGGAGCG GGGGGATTAC GTCCTGGCCG TGAAGTGGGG AGAGGAGCAC
    ATCCCCGGCA GTCCGTTCCA CGTCACCGTG CCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31629
    Clone ID Related Accession (Same CDS sequence) XM_005222908.3
    Accession Version XM_005222908.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product filamin-B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222908.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA
    GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA
    GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG
    ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT
    GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG
    GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC
    ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA
    TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG
    GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC
    AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT
    GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG
    CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC
    ACTGTCATGG TAACAGAAGA GGCCTATGTC CCAGTGAGCA ACATGAACGG CCTGGGATTT
    AAGCCTTTCG ACTTGGTCAT TCCATTTGCA GTCAGGAAAG GAGAAATCAC CGGAGAGGTC
    CACATGCCTT CCGGGAAGAC GGCCAAGCCC GAGATTGTGG ACAACAAGGA CGGCACAGTC
    ACTGTCAGAT ACGCCCCCAC GGAGGTTGGC CTCCACGAGA TGCACATCAA GTACATGGGC
    AGCCACATTC CTGAGAGCCC CCTCCAGTTC TACGTGAACT ACCCCAACAG TGGGAGCGTG
    TCTGCGTACG GCCCAGGCCT GGTGTACGGA GTGGCCAACA AAACCGCCAC CTTCACCATC
    GTCACCGAAG ACGCCGGAGA AGGTGGCCTG GACTTGGCTA TCGAGGGACC CTCAAAGGCC
    GAAATCAGCT GTATTGACAA CAAAGATGGG ACGTGCACGG TGACTTACCT GCCCACCTTG
    CCGGGCGACT ACAGCATCCT GGTCAAGTAC AATGACAAAC ACATCCCTGG CAGCCCCTTC
    ACAGCCAAGA TCACAGACGA CAGCAGACGG TGCTCTCAGG TGAAGCTGGG CTCAGCCGCC
    GACTTCTTGC TTGACATCAG CGAGACTGAC CTCAGCACCT TGACGGCCAG CATCAAGGCC
    CCGTCCGGTC GTGACGAGCC TTGTCTCCTG AAGAGGCTGC CCAACAATCA CATCGGCATC
    TCCTTCATTC CCCGGGAGGT GGGTGAACAC CTGGTCAGCA TCAAGAAGAA CGGCAACCAC
    GTGGCCAACA GCCCCGTATC CATCATGGTG GTCCAGTCGG AGATTGGGGA TGCCCGCAGA
    GCCAAAGTCT ATGGCCGTGG CCTGTCAGAA GGCCGGACTT TCGAGATGTC TGACTTCATT
    GTGGACACGA GAGATGCAGG TTACGGAGGC ATCTCCTTGG CGGTGGAAGG CCCTAGCAAG
    GTGGACATCC AGACCGAGGA CCTGGAGGAC GGCACCTGCA AGGTCTCCTA CTTCCCCACT
    GTGCCTGGGG TTTATATCGT CTCCACCAAG TTCGCTGACG AGCACGTGCC TGGGAGCCCG
    TTCACCGTGA AAATCAGCGG GGAGGGAAGA GTCAAGGAGA GCATCACCCG TACCAGCCGA
    GCCCCGTCTG TGGCCACTGT TGGCAGCATC TGTGACCTGA ACCTGAAAAT CCCAGAAATT
    GACAGCAGTG ACATGTCGGC CCACGTCACC AGCCCCTCTG GCCGCGTGAC CGAGGCAGAG
    ATCGTGCCGG TGGGGAAGAA CTCGCACTGC GTCCGGTTCG TGCCCCAGGA GATGGGCGTT
    CACACCGTCA GCGTCAAGTA CCGTGGCCAG CACGTCACTG GCAGCCCCTT CCAGTTCACT
    GTGGGGCCGC TGGGCGAGGG GGGTGCCCAC AAGGTCCGGG CAGGCGGCCC TGGCCTGGAG
    CGAGGAGAAG CCGGAGTTCC GGCGGAGTTC AGCATCTGGA CCCGGGAGGC AGGCGCCGGG
    GGCCTCTCCA TCGCCGTGGA GGGCCCCAGT AAGGCCGAGA TTACATTCGA TGACCATAAA
    AACGGATCTT GTGGTGTGTC TTATATTGCC CAGGAACCTG GTAACTACGA GGTCTCCATC
    AAGTTCAATG ACGAGCATAT TCCCGAAAGT CCCTATGTGG TGCCGGTGAT CGCGCCCTCG
    GACGACGCCC GCCGCCTCAC TGTTCTGAGC CTTCAGGAAT CGGGATTAAA AGTTAATCAG
    CCAGCCTCCT TTGCTATAAG GTTGAACGGG GCAAAAGGCA AGATCGATGC AAAGGTGCAC
    AGCCCCTCTG GAGCCGTGGA GGAATGCCAC GTGTCTGAGC TGGAGCCAGA TAAGTATGCT
    GTTCGCTTCA TCCCCCACGA GAATGGTATC CACACGATTG ACGTCAAGTT CAACGGGAGC
    CACGTGGTTG GAAGTCCCTT CAAAGTGCGC GTCGGGGAGC CTGGACAAGC GGGGAACCCC
    GCCCTGGTGT CGGCCTATGG CGCAGGGCTT GAGGGAGGCT CCACAGGTAT CCAGTCAGAA
    TTTTTCATCA ATACCACCCG AGCAGGGCCA GGGACATTAT CCGTCACCAT CGAAGGGCCG
    TCCAAGGTTA AAATGGATTG CCAGGAAACC CCCGAAGGGT ACAAGGTCAT GTACACCCCC
    ATGGCTCCTG GAAACTACCT GATTGGCGTC AAATATGGCG GGCCCAACCA CATCGTGGGC
    AGCCCGTTCA AGGCCAAGGT GACAGGCCAG CGTCTGGTCA GCCCCGGCTC GGCCAACGAG
    ACCTCCTCCA TCCTGGTGGA GTCAGTGACC AGGTCCTCGA CGGAGACGTG CTACAGCGCC
    ATCCCCAAGG CATCCTCAGA CGCCAGCAAG GTGACCTCCA AGGGCGCAGG GCTCTCGAAG
    GCCTTCCTGG GCCAGAAGAG CTCCTTCCTG GTGGACTGCA GCAAAGCCGG TTCCAACATG
    TTGCTGATCG GGGTCCACGG GCCCACCACC CCCTGCGAAG AAGTCTCCAT GAAGCATGTG
    GGCAACCAGC AGTACAACGT GACCTACGTC GTCAAGGAGC GGGGGGATTA CGTCCTGGCC
    GTGAAGTGGG GAGAGGAGCA CATCCCCGGC AGTCCGTTCC ACGTCACCGT GCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005222965.1
    CDS177..7913
    Translation

    Target ORF information:

    RefSeq Version XM_005222908.3
    Organism Bos taurus(cattle)
    Definition Bos taurus filamin B (FLNB), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005222908.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
    ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG
    CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG
    CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG
    TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA
    GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC
    TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG
    CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC
    TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA
    GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA
    GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT
    ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC
    AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT
    GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC
    ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA
    GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG
    CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG
    AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA
    GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC
    ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC
    ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA
    ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT
    CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA
    GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA
    GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG
    GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG
    GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA
    GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC
    GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG
    TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT
    GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC
    ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG
    GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC
    ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA
    AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG
    GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG
    GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC
    CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC
    GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC
    ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT
    CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC
    ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC
    CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG
    GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC
    AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT
    AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT
    CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT
    CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG
    GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG
    ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG
    GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT
    GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC
    ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG
    TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA
    TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC
    CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC
    TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC
    CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA
    GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC
    TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC
    ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC
    AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA
    AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG
    GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT
    TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC
    CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG
    GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG
    GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT
    CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT
    GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA
    TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC
    CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG
    CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG
    GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA
    GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA
    GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG
    ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT
    GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG
    GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC
    ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA
    TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG
    GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC
    AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT
    GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG
    CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC
    ACTGTCATGG TAACAGAAGA GGCCTATGTC CCAGTGAGCA ACATGAACGG CCTGGGATTT
    AAGCCTTTCG ACTTGGTCAT TCCATTTGCA GTCAGGAAAG GAGAAATCAC CGGAGAGGTC
    CACATGCCTT CCGGGAAGAC GGCCAAGCCC GAGATTGTGG ACAACAAGGA CGGCACAGTC
    ACTGTCAGAT ACGCCCCCAC GGAGGTTGGC CTCCACGAGA TGCACATCAA GTACATGGGC
    AGCCACATTC CTGAGAGCCC CCTCCAGTTC TACGTGAACT ACCCCAACAG TGGGAGCGTG
    TCTGCGTACG GCCCAGGCCT GGTGTACGGA GTGGCCAACA AAACCGCCAC CTTCACCATC
    GTCACCGAAG ACGCCGGAGA AGGTGGCCTG GACTTGGCTA TCGAGGGACC CTCAAAGGCC
    GAAATCAGCT GTATTGACAA CAAAGATGGG ACGTGCACGG TGACTTACCT GCCCACCTTG
    CCGGGCGACT ACAGCATCCT GGTCAAGTAC AATGACAAAC ACATCCCTGG CAGCCCCTTC
    ACAGCCAAGA TCACAGACGA CAGCAGACGG TGCTCTCAGG TGAAGCTGGG CTCAGCCGCC
    GACTTCTTGC TTGACATCAG CGAGACTGAC CTCAGCACCT TGACGGCCAG CATCAAGGCC
    CCGTCCGGTC GTGACGAGCC TTGTCTCCTG AAGAGGCTGC CCAACAATCA CATCGGCATC
    TCCTTCATTC CCCGGGAGGT GGGTGAACAC CTGGTCAGCA TCAAGAAGAA CGGCAACCAC
    GTGGCCAACA GCCCCGTATC CATCATGGTG GTCCAGTCGG AGATTGGGGA TGCCCGCAGA
    GCCAAAGTCT ATGGCCGTGG CCTGTCAGAA GGCCGGACTT TCGAGATGTC TGACTTCATT
    GTGGACACGA GAGATGCAGG TTACGGAGGC ATCTCCTTGG CGGTGGAAGG CCCTAGCAAG
    GTGGACATCC AGACCGAGGA CCTGGAGGAC GGCACCTGCA AGGTCTCCTA CTTCCCCACT
    GTGCCTGGGG TTTATATCGT CTCCACCAAG TTCGCTGACG AGCACGTGCC TGGGAGCCCG
    TTCACCGTGA AAATCAGCGG GGAGGGAAGA GTCAAGGAGA GCATCACCCG TACCAGCCGA
    GCCCCGTCTG TGGCCACTGT TGGCAGCATC TGTGACCTGA ACCTGAAAAT CCCAGAAATT
    GACAGCAGTG ACATGTCGGC CCACGTCACC AGCCCCTCTG GCCGCGTGAC CGAGGCAGAG
    ATCGTGCCGG TGGGGAAGAA CTCGCACTGC GTCCGGTTCG TGCCCCAGGA GATGGGCGTT
    CACACCGTCA GCGTCAAGTA CCGTGGCCAG CACGTCACTG GCAGCCCCTT CCAGTTCACT
    GTGGGGCCGC TGGGCGAGGG GGGTGCCCAC AAGGTCCGGG CAGGCGGCCC TGGCCTGGAG
    CGAGGAGAAG CCGGAGTTCC GGCGGAGTTC AGCATCTGGA CCCGGGAGGC AGGCGCCGGG
    GGCCTCTCCA TCGCCGTGGA GGGCCCCAGT AAGGCCGAGA TTACATTCGA TGACCATAAA
    AACGGATCTT GTGGTGTGTC TTATATTGCC CAGGAACCTG GTAACTACGA GGTCTCCATC
    AAGTTCAATG ACGAGCATAT TCCCGAAAGT CCCTATGTGG TGCCGGTGAT CGCGCCCTCG
    GACGACGCCC GCCGCCTCAC TGTTCTGAGC CTTCAGGAAT CGGGATTAAA AGTTAATCAG
    CCAGCCTCCT TTGCTATAAG GTTGAACGGG GCAAAAGGCA AGATCGATGC AAAGGTGCAC
    AGCCCCTCTG GAGCCGTGGA GGAATGCCAC GTGTCTGAGC TGGAGCCAGA TAAGTATGCT
    GTTCGCTTCA TCCCCCACGA GAATGGTATC CACACGATTG ACGTCAAGTT CAACGGGAGC
    CACGTGGTTG GAAGTCCCTT CAAAGTGCGC GTCGGGGAGC CTGGACAAGC GGGGAACCCC
    GCCCTGGTGT CGGCCTATGG CGCAGGGCTT GAGGGAGGCT CCACAGGTAT CCAGTCAGAA
    TTTTTCATCA ATACCACCCG AGCAGGGCCA GGGACATTAT CCGTCACCAT CGAAGGGCCG
    TCCAAGGTTA AAATGGATTG CCAGGAAACC CCCGAAGGGT ACAAGGTCAT GTACACCCCC
    ATGGCTCCTG GAAACTACCT GATTGGCGTC AAATATGGCG GGCCCAACCA CATCGTGGGC
    AGCCCGTTCA AGGCCAAGGT GACAGGCCAG CGTCTGGTCA GCCCCGGCTC GGCCAACGAG
    ACCTCCTCCA TCCTGGTGGA GTCAGTGACC AGGTCCTCGA CGGAGACGTG CTACAGCGCC
    ATCCCCAAGG CATCCTCAGA CGCCAGCAAG GTGACCTCCA AGGGCGCAGG GCTCTCGAAG
    GCCTTCCTGG GCCAGAAGAG CTCCTTCCTG GTGGACTGCA GCAAAGCCGG TTCCAACATG
    TTGCTGATCG GGGTCCACGG GCCCACCACC CCCTGCGAAG AAGTCTCCAT GAAGCATGTG
    GGCAACCAGC AGTACAACGT GACCTACGTC GTCAAGGAGC GGGGGGATTA CGTCCTGGCC
    GTGAAGTGGG GAGAGGAGCA CATCCCCGGC AGTCCGTTCC ACGTCACCGT GCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.