FLNB gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005222905.3	XP_005222962.1	filamin-B isoform X2
XM_005222908.3	XP_005222965.1	filamin-B isoform X5
NM_001191460.2	NP_001178389.1	filamin-B
XM_005222905.4	XP_005222962.1	filamin-B isoform X2
XM_005222906.4	XP_005222963.1	filamin-B isoform X3
XM_005222906.3	XP_005222963.1	filamin-B isoform X3
NM_001191460.3	NP_001178389.1	filamin-B
XM_005222907.4	XP_005222964.1	filamin-B isoform X4
XM_005222908.4	XP_005222965.1	filamin-B isoform X5
NM_001191460.1	NP_001178389.1	filamin-B
XM_005222904.3	XP_005222961.1	filamin-B isoform X1
XM_005222907.3	XP_005222964.1	filamin-B isoform X4
XM_005222904.4	XP_005222961.1	filamin-B isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa216199	NM_001191460.2 Latest version!	Bos taurus filamin B (FLNB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBa229111	XM_005222904.4 Latest version!	Bos taurus filamin B (FLNB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa229112	XM_005222905.4 Latest version!	Bos taurus filamin B (FLNB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa229113	XM_005222906.4 Latest version!	Bos taurus filamin B (FLNB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa229114	XM_005222907.4 Latest version!	Bos taurus filamin B (FLNB), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa229115	XM_005222908.4 Latest version!	Bos taurus filamin B (FLNB), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa40688	NM_001191460.3 Latest version!	Bos taurus filamin B (FLNB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa31625	XM_005222904.3 Latest version!	Bos taurus filamin B (FLNB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa31626	XM_005222905.3 Latest version!	Bos taurus filamin B (FLNB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa31627	XM_005222906.3 Latest version!	Bos taurus filamin B (FLNB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa31628	XM_005222907.3 Latest version!	Bos taurus filamin B (FLNB), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OBa31629	XM_005222908.3 Latest version!	Bos taurus filamin B (FLNB), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
View Old Accession Versions >>
OBa40688	NM_001191460.1	Bos taurus filamin B, beta (FLNB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
Hide Old Accession Versions >>

FLNB ( NM_001191460.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178389.1 Bos taurus filamin B (FLNB), mRNA.

CloneID	OBa216199
Clone ID Related Accession (Same CDS sequence)	NM_001191460.2
Accession Version	NM_001191460.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	filamin-B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02054297.1. On Jun 30, 2017 this sequence version replaced NM_001191460.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA 
GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG 
AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC 
CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC 
AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC 
TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA 
GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC 
GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG 
ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA 
CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA 
ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT 
TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC 
GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC 
TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC 
GCAGCTGTGA AGGAAGTACC GGTAAATGCA TGTCCCCCTG GATTCAGGCC CTGGGTAACA 
GAAGAGGCCT ATGTCCCAGT GAGCAACATG AACGGCCTGG GATTTAAGCC TTTCGACTTG 
GTCATTCCAT TTGCAGTCAG GAAAGGAGAA ATCACCGGAG AGGTCCACAT GCCTTCCGGG 
AAGACGGCCA AGCCCGAGAT TGTGGACAAC AAGGACGGCA CAGTCACTGT CAGATACGCC 
CCCACGGAGG TTGGCCTCCA CGAGATGCAC ATCAAGTACA TGGGCAGCCA CATTCCTGAG 
AGCCCCCTCC AGTTCTACGT GAACTACCCC AACAGTGGGA GCGTGTCTGC GTACGGCCCA 
GGCCTGGTGT ACGGAGTGGC CAACAAAACC GCCACCTTCA CCATCGTCAC CGAAGACGCC 
GGAGAAGGTG GCCTGGACTT GGCTATCGAG GGACCCTCAA AGGCCGAAAT CAGCTGTATT 
GACAACAAAG ATGGGACGTG CACGGTGACT TACCTGCCCA CCTTGCCGGG CGACTACAGC 
ATCCTGGTCA AGTACAATGA CAAACACATC CCTGGCAGCC CCTTCACAGC CAAGATCACA 
GACGACAGCA GACGGTGCTC TCAGGTGAAG CTGGGCTCAG CCGCCGACTT CTTGCTTGAC 
ATCAGCGAGA CTGACCTCAG CACCTTGACG GCCAGCATCA AGGCCCCGTC CGGTCGTGAC 
GAGCCTTGTC TCCTGAAGAG GCTGCCCAAC AATCACATCG GCATCTCCTT CATTCCCCGG 
GAGGTGGGTG AACACCTGGT CAGCATCAAG AAGAACGGCA ACCACGTGGC CAACAGCCCC 
GTATCCATCA TGGTGGTCCA GTCGGAGATT GGGGATGCCC GCAGAGCCAA AGTCTATGGC 
CGTGGCCTGT CAGAAGGCCG GACTTTCGAG ATGTCTGACT TCATTGTGGA CACGAGAGAT 
GCAGGTTACG GAGGCATCTC CTTGGCGGTG GAAGGCCCTA GCAAGGTGGA CATCCAGACC 
GAGGACCTGG AGGACGGCAC CTGCAAGGTC TCCTACTTCC CCACTGTGCC TGGGGTTTAT 
ATCGTCTCCA CCAAGTTCGC TGACGAGCAC GTGCCTGGGA GCCCGTTCAC CGTGAAAATC 
AGCGGGGAGG GAAGAGTCAA GGAGAGCATC ACCCGTACCA GCCGAGCCCC GTCTGTGGCC 
ACTGTTGGCA GCATCTGTGA CCTGAACCTG AAAATCCCAG AAATTGACAG CAGTGACATG 
TCGGCCCACG TCACCAGCCC CTCTGGCCGC GTGACCGAGG CAGAGATCGT GCCGGTGGGG 
AAGAACTCGC ACTGCGTCCG GTTCGTGCCC CAGGAGATGG GCGTTCACAC CGTCAGCGTC 
AAGTACCGTG GCCAGCACGT CACTGGCAGC CCCTTCCAGT TCACTGTGGG GCCGCTGGGC 
GAGGGGGGTG CCCACAAGGT CCGGGCAGGC GGCCCTGGCC TGGAGCGAGG AGAAGCCGGA 
GTTCCGGCGG AGTTCAGCAT CTGGACCCGG GAGGCAGGCG CCGGGGGCCT CTCCATCGCC 
GTGGAGGGCC CCAGTAAGGC CGAGATTACA TTCGATGACC ATAAAAACGG ATCTTGTGGT 
GTGTCTTATA TTGCCCAGGA ACCTGGTAAC TACGAGGTCT CCATCAAGTT CAATGACGAG 
CATATTCCCG AAAGTCCCTA TGTGGTGCCG GTGATCGCGC CCTCGGACGA CGCCCGCCGC 
CTCACTGTTC TGAGCCTTCA GGAATCGGGA TTAAAAGTTA ATCAGCCAGC CTCCTTTGCT 
ATAAGGTTGA ACGGGGCAAA AGGCAAGATC GATGCAAAGG TGCACAGCCC CTCTGGAGCC 
GTGGAGGAAT GCCACGTGTC TGAGCTGGAG CCAGATAAGT ATGCTGTTCG CTTCATCCCC 
CACGAGAATG GTATCCACAC GATTGACGTC AAGTTCAACG GGAGCCACGT GGTTGGAAGT 
CCCTTCAAAG TGCGCGTCGG GGAGCCTGGA CAAGCGGGGA ACCCCGCCCT GGTGTCGGCC 
TATGGCGCAG GGCTTGAGGG AGGCTCCACA GGTATCCAGT CAGAATTTTT CATCAATACC 
ACCCGAGCAG GGCCAGGGAC ATTATCCGTC ACCATCGAAG GGCCGTCCAA GGTTAAAATG 
GATTGCCAGG AAACCCCCGA AGGGTACAAG GTCATGTACA CCCCCATGGC TCCTGGAAAC 
TACCTGATTG GCGTCAAATA TGGCGGGCCC AACCACATCG TGGGCAGCCC GTTCAAGGCC 
AAGGTGACAG GCCAGCGTCT GGTCAGCCCC GGCTCGGCCA ACGAGACCTC CTCCATCCTG 
GTGGAGTCAG TGACCAGGTC CTCGACGGAG ACGTGCTACA GCGCCATCCC CAAGGCATCC 
TCAGACGCCA GCAAGGTGAC CTCCAAGGGC GCAGGGCTCT CGAAGGCCTT CCTGGGCCAG 
AAGAGCTCCT TCCTGGTGGA CTGCAGCAAA GCCGGTTCCA ACATGTTGCT GATCGGGGTC 
CACGGGCCCA CCACCCCCTG CGAAGAAGTC TCCATGAAGC ATGTGGGCAA CCAGCAGTAC 
AACGTGACCT ACGTCGTCAA GGAGCGGGGG GATTACGTCC TGGCCGTGAA GTGGGGAGAG 
GAGCACATCC CCGGCAGTCC GTTCCACGTC ACCGTGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178389.1
CDS	133..8034
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKHMYRKYHQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPRKPVDNAREAMQQADDWLGVPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEALVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEVASNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDRGNQVYRCVYKPMQPGPHVVKVSFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGIRIRETADFKVDTKAAGSGELGVTIKGPKGLEELVKQKGFQDGVYAFEYYPSTQGKYSVSITWGGHHIQKSPFEVQVGPEAGMQKVRAWGPGLHGGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPFMAYIHPASGDYNPDLVQAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGDGQPIDIQMKSRMDGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVSIGQGSHPQKVKVFGPGVERSGLKAHEPTHFTVDCTEAGEGDVSVGIKCDARVLSDEEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPTSPFRVKVDPSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFSSPGPGDAVKDLDIIDNYDYSHTVKYTPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSRIKINGLENRVEVGKDQEFAVDTRGAGGQGKLDVTILSPSRKVVPCLVAPMAGREGSTAKFIPREEGLYAIDLTYDGHPVPGSPYVVEASLPPDPTKVKAHGPGLRGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDPSKVVASGPGLEHGKVGEPGLLSVDCSEAGPGTLGLEAVSDSGAKAEVCIENNKDGTYAVTYVPLTAGMYTLTMKYGGELVPHFPTRVKVEPAVDTSRVKVFGPGIEGKDVFREATTDFTVDSRPLTQVGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPVPNSPFKVAVTEGCQPSRVQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYVPFAPGDYDVNITYGGAHIPGSPFRVPVKDVVDPSKVKIVGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVMGPRADQTISWSRRSPLKAISGFFKRDRKGDFMETGLVEPVNVVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSTYGVPASLPVEFAIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQTGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMATDGEVAAVKEVPVNACPPGFRPWVTEEAYVPVSNMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTAKPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEIDSSDMSAHVTSPSGRVTEAEIVPVGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAHKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYVVPVIAPSDDARRLTVLSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGAGLEGGSTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFLGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP

Target ORF information:

RefSeq Version	NM_001191460.2
Organism	Bos taurus(cattle)
Definition	Bos taurus filamin B (FLNB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191460.2

ORF Insert Sequence:

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA 
GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG 
AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC 
CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC 
AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC 
TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA 
GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC 
GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG 
ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA 
CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA 
ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT 
TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC 
GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC 
TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC 
GCAGCTGTGA AGGAAGTACC GGTAAATGCA TGTCCCCCTG GATTCAGGCC CTGGGTAACA 
GAAGAGGCCT ATGTCCCAGT GAGCAACATG AACGGCCTGG GATTTAAGCC TTTCGACTTG 
GTCATTCCAT TTGCAGTCAG GAAAGGAGAA ATCACCGGAG AGGTCCACAT GCCTTCCGGG 
AAGACGGCCA AGCCCGAGAT TGTGGACAAC AAGGACGGCA CAGTCACTGT CAGATACGCC 
CCCACGGAGG TTGGCCTCCA CGAGATGCAC ATCAAGTACA TGGGCAGCCA CATTCCTGAG 
AGCCCCCTCC AGTTCTACGT GAACTACCCC AACAGTGGGA GCGTGTCTGC GTACGGCCCA 
GGCCTGGTGT ACGGAGTGGC CAACAAAACC GCCACCTTCA CCATCGTCAC CGAAGACGCC 
GGAGAAGGTG GCCTGGACTT GGCTATCGAG GGACCCTCAA AGGCCGAAAT CAGCTGTATT 
GACAACAAAG ATGGGACGTG CACGGTGACT TACCTGCCCA CCTTGCCGGG CGACTACAGC 
ATCCTGGTCA AGTACAATGA CAAACACATC CCTGGCAGCC CCTTCACAGC CAAGATCACA 
GACGACAGCA GACGGTGCTC TCAGGTGAAG CTGGGCTCAG CCGCCGACTT CTTGCTTGAC 
ATCAGCGAGA CTGACCTCAG CACCTTGACG GCCAGCATCA AGGCCCCGTC CGGTCGTGAC 
GAGCCTTGTC TCCTGAAGAG GCTGCCCAAC AATCACATCG GCATCTCCTT CATTCCCCGG 
GAGGTGGGTG AACACCTGGT CAGCATCAAG AAGAACGGCA ACCACGTGGC CAACAGCCCC 
GTATCCATCA TGGTGGTCCA GTCGGAGATT GGGGATGCCC GCAGAGCCAA AGTCTATGGC 
CGTGGCCTGT CAGAAGGCCG GACTTTCGAG ATGTCTGACT TCATTGTGGA CACGAGAGAT 
GCAGGTTACG GAGGCATCTC CTTGGCGGTG GAAGGCCCTA GCAAGGTGGA CATCCAGACC 
GAGGACCTGG AGGACGGCAC CTGCAAGGTC TCCTACTTCC CCACTGTGCC TGGGGTTTAT 
ATCGTCTCCA CCAAGTTCGC TGACGAGCAC GTGCCTGGGA GCCCGTTCAC CGTGAAAATC 
AGCGGGGAGG GAAGAGTCAA GGAGAGCATC ACCCGTACCA GCCGAGCCCC GTCTGTGGCC 
ACTGTTGGCA GCATCTGTGA CCTGAACCTG AAAATCCCAG AAATTGACAG CAGTGACATG 
TCGGCCCACG TCACCAGCCC CTCTGGCCGC GTGACCGAGG CAGAGATCGT GCCGGTGGGG 
AAGAACTCGC ACTGCGTCCG GTTCGTGCCC CAGGAGATGG GCGTTCACAC CGTCAGCGTC 
AAGTACCGTG GCCAGCACGT CACTGGCAGC CCCTTCCAGT TCACTGTGGG GCCGCTGGGC 
GAGGGGGGTG CCCACAAGGT CCGGGCAGGC GGCCCTGGCC TGGAGCGAGG AGAAGCCGGA 
GTTCCGGCGG AGTTCAGCAT CTGGACCCGG GAGGCAGGCG CCGGGGGCCT CTCCATCGCC 
GTGGAGGGCC CCAGTAAGGC CGAGATTACA TTCGATGACC ATAAAAACGG ATCTTGTGGT 
GTGTCTTATA TTGCCCAGGA ACCTGGTAAC TACGAGGTCT CCATCAAGTT CAATGACGAG 
CATATTCCCG AAAGTCCCTA TGTGGTGCCG GTGATCGCGC CCTCGGACGA CGCCCGCCGC 
CTCACTGTTC TGAGCCTTCA GGAATCGGGA TTAAAAGTTA ATCAGCCAGC CTCCTTTGCT 
ATAAGGTTGA ACGGGGCAAA AGGCAAGATC GATGCAAAGG TGCACAGCCC CTCTGGAGCC 
GTGGAGGAAT GCCACGTGTC TGAGCTGGAG CCAGATAAGT ATGCTGTTCG CTTCATCCCC 
CACGAGAATG GTATCCACAC GATTGACGTC AAGTTCAACG GGAGCCACGT GGTTGGAAGT 
CCCTTCAAAG TGCGCGTCGG GGAGCCTGGA CAAGCGGGGA ACCCCGCCCT GGTGTCGGCC 
TATGGCGCAG GGCTTGAGGG AGGCTCCACA GGTATCCAGT CAGAATTTTT CATCAATACC 
ACCCGAGCAG GGCCAGGGAC ATTATCCGTC ACCATCGAAG GGCCGTCCAA GGTTAAAATG 
GATTGCCAGG AAACCCCCGA AGGGTACAAG GTCATGTACA CCCCCATGGC TCCTGGAAAC 
TACCTGATTG GCGTCAAATA TGGCGGGCCC AACCACATCG TGGGCAGCCC GTTCAAGGCC 
AAGGTGACAG GCCAGCGTCT GGTCAGCCCC GGCTCGGCCA ACGAGACCTC CTCCATCCTG 
GTGGAGTCAG TGACCAGGTC CTCGACGGAG ACGTGCTACA GCGCCATCCC CAAGGCATCC 
TCAGACGCCA GCAAGGTGAC CTCCAAGGGC GCAGGGCTCT CGAAGGCCTT CCTGGGCCAG 
AAGAGCTCCT TCCTGGTGGA CTGCAGCAAA GCCGGTTCCA ACATGTTGCT GATCGGGGTC 
CACGGGCCCA CCACCCCCTG CGAAGAAGTC TCCATGAAGC ATGTGGGCAA CCAGCAGTAC 
AACGTGACCT ACGTCGTCAA GGAGCGGGGG GATTACGTCC TGGCCGTGAA GTGGGGAGAG 
GAGCACATCC CCGGCAGTCC GTTCCACGTC ACCGTGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_005222904.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222961.1 Bos taurus filamin B (FLNB), transcript variant X1, mRNA.

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CloneID	OBa229111
Clone ID Related Accession (Same CDS sequence)	XM_005222904.4
Accession Version	XM_005222904.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	filamin-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222904.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA 
GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG 
AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC 
CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC 
AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC 
TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA 
GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC 
GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG 
ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA 
CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA 
ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT 
TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC 
GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC 
TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC 
GCAGCTGTGA AGGAAGTACC GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC 
GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC 
ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG 
GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC 
AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC 
AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC 
ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA 
CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC 
CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT 
GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG 
GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC 
AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT 
CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG 
AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG 
GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG 
TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA 
GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC 
TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG 
CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC 
CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA 
ATCCCAGAAA TTGACAGCAG TGACATGTCA GCCCACGTCA CCAGCCCCTC TGGCCGCGTG 
ACCGAGGCGG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG 
GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC 
TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC 
CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG 
GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC 
GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC 
GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG 
ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA 
AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT 
GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA 
GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG 
TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA 
GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT 
ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC 
ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC 
ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC 
CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC 
TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG 
TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA 
GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC 
GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC 
ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT 
TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC 
GTGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222961.1
CDS	178..8046
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKHMYRKYHQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPRKPVDNAREAMQQADDWLGVPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEALVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEVASNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDRGNQVYRCVYKPMQPGPHVVKVSFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGIRIRETADFKVDTKAAGSGELGVTIKGPKGLEELVKQKGFQDGVYAFEYYPSTQGKYSVSITWGGHHIQKSPFEVQVGPEAGMQKVRAWGPGLHGGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPFMAYIHPASGDYNPDLVQAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGDGQPIDIQMKSRMDGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVSIGQGSHPQKVKVFGPGVERSGLKAHEPTHFTVDCTEAGEGDVSVGIKCDARVLSDEEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPTSPFRVKVDPSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFSSPGPGDAVKDLDIIDNYDYSHTVKYTPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSRIKINGLENRVEVGKDQEFAVDTRGAGGQGKLDVTILSPSRKVVPCLVAPMAGREGSTAKFIPREEGLYAIDLTYDGHPVPGSPYVVEASLPPDPTKVKAHGPGLRGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDPSKVVASGPGLEHGKVGEPGLLSVDCSEAGPGTLGLEAVSDSGAKAEVCIENNKDGTYAVTYVPLTAGMYTLTMKYGGELVPHFPTRVKVEPAVDTSRVKVFGPGIEGKDVFREATTDFTVDSRPLTQVGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPVPNSPFKVAVTEGCQPSRVQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYVPFAPGDYDVNITYGGAHIPGSPFRVPVKDVVDPSKVKIVGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVMGPRADQTISWSRRSPLKAISGFFKRDRKGDFMETGLVEPVNVVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSTYGVPASLPVEFAIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQTGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMATDGEVAAVKEVPVTEEAYVPVSNMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTAKPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEIDSSDMSAHVTSPSGRVTEAEIVPVGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAHKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYVVPVIAPSDDARRLTVLSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGAGLEGGSTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFLGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP

Target ORF information:

RefSeq Version	XM_005222904.4
Organism	Bos taurus(cattle)
Definition	Bos taurus filamin B (FLNB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222904.4

ORF Insert Sequence:

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA 
GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG 
AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC 
CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC 
AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC 
TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA 
GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC 
GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG 
ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA 
CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA 
ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT 
TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC 
GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC 
TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC 
GCAGCTGTGA AGGAAGTACC GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC 
GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC 
ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG 
GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC 
AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC 
AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC 
ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA 
CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC 
CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT 
GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG 
GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC 
AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT 
CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG 
AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG 
GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG 
TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA 
GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC 
TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG 
CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC 
CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA 
ATCCCAGAAA TTGACAGCAG TGACATGTCA GCCCACGTCA CCAGCCCCTC TGGCCGCGTG 
ACCGAGGCGG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG 
GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC 
TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC 
CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG 
GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC 
GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC 
GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG 
ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA 
AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT 
GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA 
GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG 
TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA 
GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT 
ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC 
ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC 
ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC 
CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC 
TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG 
TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA 
GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC 
GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC 
ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT 
TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC 
GTGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_005222905.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222962.1 Bos taurus filamin B (FLNB), transcript variant X2, mRNA.

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CloneID	OBa229112
Clone ID Related Accession (Same CDS sequence)	XM_005222905.4
Accession Version	XM_005222905.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	filamin-B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222905.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA 
GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG 
AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC 
CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC 
AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC 
TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA 
GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC 
GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG 
ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA 
CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA 
ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT 
TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC 
GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC 
TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGTAACAGA AGAGGCCTAT 
GTCCCAGTGA GCAACATGAA CGGCCTGGGA TTTAAGCCTT TCGACTTGGT CATTCCATTT 
GCAGTCAGGA AAGGAGAAAT CACCGGAGAG GTCCACATGC CTTCCGGGAA GACGGCCAAG 
CCCGAGATTG TGGACAACAA GGACGGCACA GTCACTGTCA GATACGCCCC CACGGAGGTT 
GGCCTCCACG AGATGCACAT CAAGTACATG GGCAGCCACA TTCCTGAGAG CCCCCTCCAG 
TTCTACGTGA ACTACCCCAA CAGTGGGAGC GTGTCTGCGT ACGGCCCAGG CCTGGTGTAC 
GGAGTGGCCA ACAAAACCGC CACCTTCACC ATCGTCACCG AAGACGCCGG AGAAGGTGGC 
CTGGACTTGG CTATCGAGGG ACCCTCAAAG GCCGAAATCA GCTGTATTGA CAACAAAGAT 
GGGACGTGCA CGGTGACTTA CCTGCCCACC TTGCCGGGCG ACTACAGCAT CCTGGTCAAG 
TACAATGACA AACACATCCC TGGCAGCCCC TTCACAGCCA AGATCACAGA CGACAGCAGA 
CGGTGCTCTC AGGTGAAGCT GGGCTCAGCC GCCGACTTCT TGCTTGACAT CAGCGAGACT 
GACCTCAGCA CCTTGACGGC CAGCATCAAG GCCCCGTCCG GTCGTGACGA GCCTTGTCTC 
CTGAAGAGGC TGCCCAACAA TCACATCGGC ATCTCCTTCA TTCCCCGGGA GGTGGGTGAA 
CACCTGGTCA GCATCAAGAA GAACGGCAAC CACGTGGCCA ACAGCCCCGT ATCCATCATG 
GTGGTCCAGT CGGAGATTGG GGATGCCCGC AGAGCCAAAG TCTATGGCCG TGGCCTGTCA 
GAAGGCCGGA CTTTCGAGAT GTCTGACTTC ATTGTGGACA CGAGAGATGC AGGTTACGGA 
GGCATCTCCT TGGCGGTGGA AGGCCCTAGC AAGGTGGACA TCCAGACCGA GGACCTGGAG 
GACGGCACCT GCAAGGTCTC CTACTTCCCC ACTGTGCCTG GGGTTTATAT CGTCTCCACC 
AAGTTCGCTG ACGAGCACGT GCCTGGGAGC CCGTTCACCG TGAAAATCAG CGGGGAGGGA 
AGAGTCAAGG AGAGCATCAC CCGTACCAGC CGAGCCCCGT CTGTGGCCAC TGTTGGCAGC 
ATCTGTGACC TGAACCTGAA AATCCCAGAA ATTGACAGCA GTGACATGTC AGCCCACGTC 
ACCAGCCCCT CTGGCCGCGT GACCGAGGCG GAGATCGTGC CGGTGGGGAA GAACTCGCAC 
TGCGTCCGGT TCGTGCCCCA GGAGATGGGC GTTCACACCG TCAGCGTCAA GTACCGTGGC 
CAGCACGTCA CTGGCAGCCC CTTCCAGTTC ACTGTGGGGC CGCTGGGCGA GGGGGGTGCC 
CACAAGGTCC GGGCAGGCGG CCCTGGCCTG GAGCGAGGAG AAGCCGGAGT TCCGGCGGAG 
TTCAGCATCT GGACCCGGGA GGCAGGCGCC GGGGGCCTCT CCATCGCCGT GGAGGGCCCC 
AGTAAGGCCG AGATTACATT CGATGACCAT AAAAACGGAT CTTGTGGTGT GTCTTATATT 
GCCCAGGAAC CTGGTAACTA CGAGGTCTCC ATCAAGTTCA ATGACGAGCA TATTCCCGAA 
AGTCCCTATG TGGTGCCGGT GATCGCGCCC TCGGACGACG CCCGCCGCCT CACTGTTCTG 
AGCCTTCAGG AATCGGGATT AAAAGTTAAT CAGCCAGCCT CCTTTGCTAT AAGGTTGAAC 
GGGGCAAAAG GCAAGATCGA TGCAAAGGTG CACAGCCCCT CTGGAGCCGT GGAGGAATGC 
CACGTGTCTG AGCTGGAGCC AGATAAGTAT GCTGTTCGCT TCATCCCCCA CGAGAATGGT 
ATCCACACGA TTGACGTCAA GTTCAACGGG AGCCACGTGG TTGGAAGTCC CTTCAAAGTG 
CGCGTCGGGG AGCCTGGACA AGCGGGGAAC CCCGCCCTGG TGTCGGCCTA TGGCGCAGGG 
CTTGAGGGAG GCTCCACAGG TATCCAGTCA GAATTTTTCA TCAATACCAC CCGAGCAGGG 
CCAGGGACAT TATCCGTCAC CATCGAAGGG CCGTCCAAGG TTAAAATGGA TTGCCAGGAA 
ACCCCCGAAG GGTACAAGGT CATGTACACC CCCATGGCTC CTGGAAACTA CCTGATTGGC 
GTCAAATATG GCGGGCCCAA CCACATCGTG GGCAGCCCGT TCAAGGCCAA GGTGACAGGC 
CAGCGTCTGG TCAGCCCCGG CTCGGCCAAC GAGACCTCCT CCATCCTGGT GGAGTCAGTG 
ACCAGGTCCT CGACGGAGAC GTGCTACAGC GCCATCCCCA AGGCATCCTC AGACGCCAGC 
AAGGTGACCT CCAAGGGCGC AGGGCTCTCG AAGGCCTTCC TGGGCCAGAA GAGCTCCTTC 
CTGGTGGACT GCAGCAAAGC CGGTTCCAAC ATGTTGCTGA TCGGGGTCCA CGGGCCCACC 
ACCCCCTGCG AAGAAGTCTC CATGAAGCAT GTGGGCAACC AGCAGTACAA CGTGACCTAC 
GTCGTCAAGG AGCGGGGGGA TTACGTCCTG GCCGTGAAGT GGGGAGAGGA GCACATCCCC 
GGCAGTCCGT TCCACGTCAC CGTGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222962.1
CDS	178..8007
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKHMYRKYHQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPRKPVDNAREAMQQADDWLGVPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEALVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEVASNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDRGNQVYRCVYKPMQPGPHVVKVSFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGIRIRETADFKVDTKAAGSGELGVTIKGPKGLEELVKQKGFQDGVYAFEYYPSTQGKYSVSITWGGHHIQKSPFEVQVGPEAGMQKVRAWGPGLHGGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPFMAYIHPASGDYNPDLVQAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGDGQPIDIQMKSRMDGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVSIGQGSHPQKVKVFGPGVERSGLKAHEPTHFTVDCTEAGEGDVSVGIKCDARVLSDEEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPTSPFRVKVDPSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFSSPGPGDAVKDLDIIDNYDYSHTVKYTPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSRIKINGLENRVEVGKDQEFAVDTRGAGGQGKLDVTILSPSRKVVPCLVAPMAGREGSTAKFIPREEGLYAIDLTYDGHPVPGSPYVVEASLPPDPTKVKAHGPGLRGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDPSKVVASGPGLEHGKVGEPGLLSVDCSEAGPGTLGLEAVSDSGAKAEVCIENNKDGTYAVTYVPLTAGMYTLTMKYGGELVPHFPTRVKVEPAVDTSRVKVFGPGIEGKDVFREATTDFTVDSRPLTQVGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPVPNSPFKVAVTEGCQPSRVQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYVPFAPGDYDVNITYGGAHIPGSPFRVPVKDVVDPSKVKIVGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVMGPRADQTISWSRRSPLKAISGFFKRDRKGDFMETGLVEPVNVVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSTYGVPASLPVEFAIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQTGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMVTEEAYVPVSNMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTAKPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEIDSSDMSAHVTSPSGRVTEAEIVPVGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAHKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYVVPVIAPSDDARRLTVLSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGAGLEGGSTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFLGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP

Target ORF information:

RefSeq Version	XM_005222905.4
Organism	Bos taurus(cattle)
Definition	Bos taurus filamin B (FLNB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222905.4

ORF Insert Sequence:

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA 
GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG 
AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC 
CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC 
AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC 
TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA 
GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC 
GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG 
ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA 
CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA 
ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT 
TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC 
GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC 
TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGTAACAGA AGAGGCCTAT 
GTCCCAGTGA GCAACATGAA CGGCCTGGGA TTTAAGCCTT TCGACTTGGT CATTCCATTT 
GCAGTCAGGA AAGGAGAAAT CACCGGAGAG GTCCACATGC CTTCCGGGAA GACGGCCAAG 
CCCGAGATTG TGGACAACAA GGACGGCACA GTCACTGTCA GATACGCCCC CACGGAGGTT 
GGCCTCCACG AGATGCACAT CAAGTACATG GGCAGCCACA TTCCTGAGAG CCCCCTCCAG 
TTCTACGTGA ACTACCCCAA CAGTGGGAGC GTGTCTGCGT ACGGCCCAGG CCTGGTGTAC 
GGAGTGGCCA ACAAAACCGC CACCTTCACC ATCGTCACCG AAGACGCCGG AGAAGGTGGC 
CTGGACTTGG CTATCGAGGG ACCCTCAAAG GCCGAAATCA GCTGTATTGA CAACAAAGAT 
GGGACGTGCA CGGTGACTTA CCTGCCCACC TTGCCGGGCG ACTACAGCAT CCTGGTCAAG 
TACAATGACA AACACATCCC TGGCAGCCCC TTCACAGCCA AGATCACAGA CGACAGCAGA 
CGGTGCTCTC AGGTGAAGCT GGGCTCAGCC GCCGACTTCT TGCTTGACAT CAGCGAGACT 
GACCTCAGCA CCTTGACGGC CAGCATCAAG GCCCCGTCCG GTCGTGACGA GCCTTGTCTC 
CTGAAGAGGC TGCCCAACAA TCACATCGGC ATCTCCTTCA TTCCCCGGGA GGTGGGTGAA 
CACCTGGTCA GCATCAAGAA GAACGGCAAC CACGTGGCCA ACAGCCCCGT ATCCATCATG 
GTGGTCCAGT CGGAGATTGG GGATGCCCGC AGAGCCAAAG TCTATGGCCG TGGCCTGTCA 
GAAGGCCGGA CTTTCGAGAT GTCTGACTTC ATTGTGGACA CGAGAGATGC AGGTTACGGA 
GGCATCTCCT TGGCGGTGGA AGGCCCTAGC AAGGTGGACA TCCAGACCGA GGACCTGGAG 
GACGGCACCT GCAAGGTCTC CTACTTCCCC ACTGTGCCTG GGGTTTATAT CGTCTCCACC 
AAGTTCGCTG ACGAGCACGT GCCTGGGAGC CCGTTCACCG TGAAAATCAG CGGGGAGGGA 
AGAGTCAAGG AGAGCATCAC CCGTACCAGC CGAGCCCCGT CTGTGGCCAC TGTTGGCAGC 
ATCTGTGACC TGAACCTGAA AATCCCAGAA ATTGACAGCA GTGACATGTC AGCCCACGTC 
ACCAGCCCCT CTGGCCGCGT GACCGAGGCG GAGATCGTGC CGGTGGGGAA GAACTCGCAC 
TGCGTCCGGT TCGTGCCCCA GGAGATGGGC GTTCACACCG TCAGCGTCAA GTACCGTGGC 
CAGCACGTCA CTGGCAGCCC CTTCCAGTTC ACTGTGGGGC CGCTGGGCGA GGGGGGTGCC 
CACAAGGTCC GGGCAGGCGG CCCTGGCCTG GAGCGAGGAG AAGCCGGAGT TCCGGCGGAG 
TTCAGCATCT GGACCCGGGA GGCAGGCGCC GGGGGCCTCT CCATCGCCGT GGAGGGCCCC 
AGTAAGGCCG AGATTACATT CGATGACCAT AAAAACGGAT CTTGTGGTGT GTCTTATATT 
GCCCAGGAAC CTGGTAACTA CGAGGTCTCC ATCAAGTTCA ATGACGAGCA TATTCCCGAA 
AGTCCCTATG TGGTGCCGGT GATCGCGCCC TCGGACGACG CCCGCCGCCT CACTGTTCTG 
AGCCTTCAGG AATCGGGATT AAAAGTTAAT CAGCCAGCCT CCTTTGCTAT AAGGTTGAAC 
GGGGCAAAAG GCAAGATCGA TGCAAAGGTG CACAGCCCCT CTGGAGCCGT GGAGGAATGC 
CACGTGTCTG AGCTGGAGCC AGATAAGTAT GCTGTTCGCT TCATCCCCCA CGAGAATGGT 
ATCCACACGA TTGACGTCAA GTTCAACGGG AGCCACGTGG TTGGAAGTCC CTTCAAAGTG 
CGCGTCGGGG AGCCTGGACA AGCGGGGAAC CCCGCCCTGG TGTCGGCCTA TGGCGCAGGG 
CTTGAGGGAG GCTCCACAGG TATCCAGTCA GAATTTTTCA TCAATACCAC CCGAGCAGGG 
CCAGGGACAT TATCCGTCAC CATCGAAGGG CCGTCCAAGG TTAAAATGGA TTGCCAGGAA 
ACCCCCGAAG GGTACAAGGT CATGTACACC CCCATGGCTC CTGGAAACTA CCTGATTGGC 
GTCAAATATG GCGGGCCCAA CCACATCGTG GGCAGCCCGT TCAAGGCCAA GGTGACAGGC 
CAGCGTCTGG TCAGCCCCGG CTCGGCCAAC GAGACCTCCT CCATCCTGGT GGAGTCAGTG 
ACCAGGTCCT CGACGGAGAC GTGCTACAGC GCCATCCCCA AGGCATCCTC AGACGCCAGC 
AAGGTGACCT CCAAGGGCGC AGGGCTCTCG AAGGCCTTCC TGGGCCAGAA GAGCTCCTTC 
CTGGTGGACT GCAGCAAAGC CGGTTCCAAC ATGTTGCTGA TCGGGGTCCA CGGGCCCACC 
ACCCCCTGCG AAGAAGTCTC CATGAAGCAT GTGGGCAACC AGCAGTACAA CGTGACCTAC 
GTCGTCAAGG AGCGGGGGGA TTACGTCCTG GCCGTGAAGT GGGGAGAGGA GCACATCCCC 
GGCAGTCCGT TCCACGTCAC CGTGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_005222906.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222963.1 Bos taurus filamin B (FLNB), transcript variant X3, mRNA.

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CloneID	OBa229113
Clone ID Related Accession (Same CDS sequence)	XM_005222906.4
Accession Version	XM_005222906.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	filamin-B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222906.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA 
GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA 
GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG 
ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT 
GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG 
GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC 
ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA 
TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG 
GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC 
AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT 
GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG 
CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC 
ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AAATGCATGT 
CCCCCTGGAT TCAGGCCCTG GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC 
GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC 
ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG 
GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC 
AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC 
AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC 
ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA 
CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC 
CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT 
GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG 
GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC 
AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT 
CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG 
AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG 
GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG 
TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA 
GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC 
TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG 
CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC 
CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA 
ATCCCAGAAA TTGACAGCAG TGACATGTCA GCCCACGTCA CCAGCCCCTC TGGCCGCGTG 
ACCGAGGCGG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG 
GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC 
TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC 
CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG 
GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC 
GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC 
GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG 
ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA 
AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT 
GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA 
GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG 
TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA 
GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT 
ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC 
ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC 
ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC 
CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC 
TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG 
TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA 
GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC 
GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC 
ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT 
TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC 
GTGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222963.1
CDS	178..7986
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKHMYRKYHQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPRKPVDNAREAMQQADDWLGVPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEALVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEVASNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDRGNQVYRCVYKPMQPGPHVVKVSFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGIRIRETADFKVDTKAAGSGELGVTIKGPKGLEELVKQKGFQDGVYAFEYYPSTQGKYSVSITWGGHHIQKSPFEVQVGPEAGMQKVRAWGPGLHGGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPFMAYIHPASGDYNPDLVQAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGDGQPIDIQMKSRMDGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVSIGQGSHPQKVKVFGPGVERSGLKAHEPTHFTVDCTEAGEGDVSVGIKCDARVLSDEEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPTSPFRVKVDPSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFSSPGPGDAVKDLDIIDNYDYSHTVKYTPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSRIKINGLENRVEVGKDQEFAVDTRGAGGQGKLDVTILSPSRKVVPCLVAPMAGREGSTAKFIPREEGLYAIDLTYDGHPVPGSPYVVEASLPPDPTKVKAHGPGLRGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDPSKVVASGPGLEHGKVGEPGLLSVDCSEAGPGTLGLEAVSDSGAKAEVCIENNKDGTYAVTYVPLTAGMYTLTMKYGGELVPHFPTRVKVEPAVDTSRVKVFGPGIEGKDVFREATTDFTVDSRPLTQVGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPVPNSPFKVAVTEGCQPSRVQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYVPFAPGDYDVNITYGGAHIPGSPFRVPVKDVVDPSKVKIVGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVMGPRGLVEPVNVVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSTYGVPASLPVEFAIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQTGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMATDGEVAAVKEVPVNACPPGFRPWVTEEAYVPVSNMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTAKPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEIDSSDMSAHVTSPSGRVTEAEIVPVGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAHKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYVVPVIAPSDDARRLTVLSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGAGLEGGSTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFLGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP

Target ORF information:

RefSeq Version	XM_005222906.4
Organism	Bos taurus(cattle)
Definition	Bos taurus filamin B (FLNB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222906.4

ORF Insert Sequence:

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA 
GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA 
GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG 
ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT 
GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG 
GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC 
ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA 
TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG 
GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC 
AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT 
GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG 
CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC 
ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AAATGCATGT 
CCCCCTGGAT TCAGGCCCTG GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC 
GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC 
ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG 
GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC 
AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC 
AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC 
ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA 
CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC 
CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT 
GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG 
GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC 
AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT 
CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG 
AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG 
GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG 
TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA 
GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC 
TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG 
CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC 
CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA 
ATCCCAGAAA TTGACAGCAG TGACATGTCA GCCCACGTCA CCAGCCCCTC TGGCCGCGTG 
ACCGAGGCGG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG 
GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC 
TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC 
CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG 
GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC 
GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC 
GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG 
ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA 
AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT 
GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA 
GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG 
TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA 
GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT 
ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC 
ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC 
ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC 
CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC 
TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG 
TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA 
GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC 
GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC 
ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT 
TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC 
GTGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_005222907.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222964.1 Bos taurus filamin B (FLNB), transcript variant X4, mRNA.

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CloneID	OBa229114
Clone ID Related Accession (Same CDS sequence)	XM_005222907.4
Accession Version	XM_005222907.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	filamin-B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222907.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA 
GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA 
GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG 
ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT 
GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG 
GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC 
ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA 
TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG 
GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC 
AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT 
GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG 
CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC 
ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AACAGAAGAG 
GCCTATGTCC CAGTGAGCAA CATGAACGGC CTGGGATTTA AGCCTTTCGA CTTGGTCATT 
CCATTTGCAG TCAGGAAAGG AGAAATCACC GGAGAGGTCC ACATGCCTTC CGGGAAGACG 
GCCAAGCCCG AGATTGTGGA CAACAAGGAC GGCACAGTCA CTGTCAGATA CGCCCCCACG 
GAGGTTGGCC TCCACGAGAT GCACATCAAG TACATGGGCA GCCACATTCC TGAGAGCCCC 
CTCCAGTTCT ACGTGAACTA CCCCAACAGT GGGAGCGTGT CTGCGTACGG CCCAGGCCTG 
GTGTACGGAG TGGCCAACAA AACCGCCACC TTCACCATCG TCACCGAAGA CGCCGGAGAA 
GGTGGCCTGG ACTTGGCTAT CGAGGGACCC TCAAAGGCCG AAATCAGCTG TATTGACAAC 
AAAGATGGGA CGTGCACGGT GACTTACCTG CCCACCTTGC CGGGCGACTA CAGCATCCTG 
GTCAAGTACA ATGACAAACA CATCCCTGGC AGCCCCTTCA CAGCCAAGAT CACAGACGAC 
AGCAGACGGT GCTCTCAGGT GAAGCTGGGC TCAGCCGCCG ACTTCTTGCT TGACATCAGC 
GAGACTGACC TCAGCACCTT GACGGCCAGC ATCAAGGCCC CGTCCGGTCG TGACGAGCCT 
TGTCTCCTGA AGAGGCTGCC CAACAATCAC ATCGGCATCT CCTTCATTCC CCGGGAGGTG 
GGTGAACACC TGGTCAGCAT CAAGAAGAAC GGCAACCACG TGGCCAACAG CCCCGTATCC 
ATCATGGTGG TCCAGTCGGA GATTGGGGAT GCCCGCAGAG CCAAAGTCTA TGGCCGTGGC 
CTGTCAGAAG GCCGGACTTT CGAGATGTCT GACTTCATTG TGGACACGAG AGATGCAGGT 
TACGGAGGCA TCTCCTTGGC GGTGGAAGGC CCTAGCAAGG TGGACATCCA GACCGAGGAC 
CTGGAGGACG GCACCTGCAA GGTCTCCTAC TTCCCCACTG TGCCTGGGGT TTATATCGTC 
TCCACCAAGT TCGCTGACGA GCACGTGCCT GGGAGCCCGT TCACCGTGAA AATCAGCGGG 
GAGGGAAGAG TCAAGGAGAG CATCACCCGT ACCAGCCGAG CCCCGTCTGT GGCCACTGTT 
GGCAGCATCT GTGACCTGAA CCTGAAAATC CCAGAAATTG ACAGCAGTGA CATGTCAGCC 
CACGTCACCA GCCCCTCTGG CCGCGTGACC GAGGCGGAGA TCGTGCCGGT GGGGAAGAAC 
TCGCACTGCG TCCGGTTCGT GCCCCAGGAG ATGGGCGTTC ACACCGTCAG CGTCAAGTAC 
CGTGGCCAGC ACGTCACTGG CAGCCCCTTC CAGTTCACTG TGGGGCCGCT GGGCGAGGGG 
GGTGCCCACA AGGTCCGGGC AGGCGGCCCT GGCCTGGAGC GAGGAGAAGC CGGAGTTCCG 
GCGGAGTTCA GCATCTGGAC CCGGGAGGCA GGCGCCGGGG GCCTCTCCAT CGCCGTGGAG 
GGCCCCAGTA AGGCCGAGAT TACATTCGAT GACCATAAAA ACGGATCTTG TGGTGTGTCT 
TATATTGCCC AGGAACCTGG TAACTACGAG GTCTCCATCA AGTTCAATGA CGAGCATATT 
CCCGAAAGTC CCTATGTGGT GCCGGTGATC GCGCCCTCGG ACGACGCCCG CCGCCTCACT 
GTTCTGAGCC TTCAGGAATC GGGATTAAAA GTTAATCAGC CAGCCTCCTT TGCTATAAGG 
TTGAACGGGG CAAAAGGCAA GATCGATGCA AAGGTGCACA GCCCCTCTGG AGCCGTGGAG 
GAATGCCACG TGTCTGAGCT GGAGCCAGAT AAGTATGCTG TTCGCTTCAT CCCCCACGAG 
AATGGTATCC ACACGATTGA CGTCAAGTTC AACGGGAGCC ACGTGGTTGG AAGTCCCTTC 
AAAGTGCGCG TCGGGGAGCC TGGACAAGCG GGGAACCCCG CCCTGGTGTC GGCCTATGGC 
GCAGGGCTTG AGGGAGGCTC CACAGGTATC CAGTCAGAAT TTTTCATCAA TACCACCCGA 
GCAGGGCCAG GGACATTATC CGTCACCATC GAAGGGCCGT CCAAGGTTAA AATGGATTGC 
CAGGAAACCC CCGAAGGGTA CAAGGTCATG TACACCCCCA TGGCTCCTGG AAACTACCTG 
ATTGGCGTCA AATATGGCGG GCCCAACCAC ATCGTGGGCA GCCCGTTCAA GGCCAAGGTG 
ACAGGCCAGC GTCTGGTCAG CCCCGGCTCG GCCAACGAGA CCTCCTCCAT CCTGGTGGAG 
TCAGTGACCA GGTCCTCGAC GGAGACGTGC TACAGCGCCA TCCCCAAGGC ATCCTCAGAC 
GCCAGCAAGG TGACCTCCAA GGGCGCAGGG CTCTCGAAGG CCTTCCTGGG CCAGAAGAGC 
TCCTTCCTGG TGGACTGCAG CAAAGCCGGT TCCAACATGT TGCTGATCGG GGTCCACGGG 
CCCACCACCC CCTGCGAAGA AGTCTCCATG AAGCATGTGG GCAACCAGCA GTACAACGTG 
ACCTACGTCG TCAAGGAGCG GGGGGATTAC GTCCTGGCCG TGAAGTGGGG AGAGGAGCAC 
ATCCCCGGCA GTCCGTTCCA CGTCACCGTG CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222964.1
CDS	178..7953
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKHMYRKYHQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPRKPVDNAREAMQQADDWLGVPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEALVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEVASNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDRGNQVYRCVYKPMQPGPHVVKVSFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGIRIRETADFKVDTKAAGSGELGVTIKGPKGLEELVKQKGFQDGVYAFEYYPSTQGKYSVSITWGGHHIQKSPFEVQVGPEAGMQKVRAWGPGLHGGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPFMAYIHPASGDYNPDLVQAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGDGQPIDIQMKSRMDGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVSIGQGSHPQKVKVFGPGVERSGLKAHEPTHFTVDCTEAGEGDVSVGIKCDARVLSDEEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPTSPFRVKVDPSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFSSPGPGDAVKDLDIIDNYDYSHTVKYTPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSRIKINGLENRVEVGKDQEFAVDTRGAGGQGKLDVTILSPSRKVVPCLVAPMAGREGSTAKFIPREEGLYAIDLTYDGHPVPGSPYVVEASLPPDPTKVKAHGPGLRGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDPSKVVASGPGLEHGKVGEPGLLSVDCSEAGPGTLGLEAVSDSGAKAEVCIENNKDGTYAVTYVPLTAGMYTLTMKYGGELVPHFPTRVKVEPAVDTSRVKVFGPGIEGKDVFREATTDFTVDSRPLTQVGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPVPNSPFKVAVTEGCQPSRVQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYVPFAPGDYDVNITYGGAHIPGSPFRVPVKDVVDPSKVKIVGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVMGPRGLVEPVNVVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSTYGVPASLPVEFAIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQTGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMATDGEVAAVKEVPVTEEAYVPVSNMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTAKPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEIDSSDMSAHVTSPSGRVTEAEIVPVGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAHKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYVVPVIAPSDDARRLTVLSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGAGLEGGSTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFLGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP

Target ORF information:

RefSeq Version	XM_005222907.4
Organism	Bos taurus(cattle)
Definition	Bos taurus filamin B (FLNB), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222907.4

ORF Insert Sequence:

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA 
GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA 
GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG 
ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT 
GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG 
GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC 
ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA 
TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG 
GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC 
AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT 
GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG 
CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC 
ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AACAGAAGAG 
GCCTATGTCC CAGTGAGCAA CATGAACGGC CTGGGATTTA AGCCTTTCGA CTTGGTCATT 
CCATTTGCAG TCAGGAAAGG AGAAATCACC GGAGAGGTCC ACATGCCTTC CGGGAAGACG 
GCCAAGCCCG AGATTGTGGA CAACAAGGAC GGCACAGTCA CTGTCAGATA CGCCCCCACG 
GAGGTTGGCC TCCACGAGAT GCACATCAAG TACATGGGCA GCCACATTCC TGAGAGCCCC 
CTCCAGTTCT ACGTGAACTA CCCCAACAGT GGGAGCGTGT CTGCGTACGG CCCAGGCCTG 
GTGTACGGAG TGGCCAACAA AACCGCCACC TTCACCATCG TCACCGAAGA CGCCGGAGAA 
GGTGGCCTGG ACTTGGCTAT CGAGGGACCC TCAAAGGCCG AAATCAGCTG TATTGACAAC 
AAAGATGGGA CGTGCACGGT GACTTACCTG CCCACCTTGC CGGGCGACTA CAGCATCCTG 
GTCAAGTACA ATGACAAACA CATCCCTGGC AGCCCCTTCA CAGCCAAGAT CACAGACGAC 
AGCAGACGGT GCTCTCAGGT GAAGCTGGGC TCAGCCGCCG ACTTCTTGCT TGACATCAGC 
GAGACTGACC TCAGCACCTT GACGGCCAGC ATCAAGGCCC CGTCCGGTCG TGACGAGCCT 
TGTCTCCTGA AGAGGCTGCC CAACAATCAC ATCGGCATCT CCTTCATTCC CCGGGAGGTG 
GGTGAACACC TGGTCAGCAT CAAGAAGAAC GGCAACCACG TGGCCAACAG CCCCGTATCC 
ATCATGGTGG TCCAGTCGGA GATTGGGGAT GCCCGCAGAG CCAAAGTCTA TGGCCGTGGC 
CTGTCAGAAG GCCGGACTTT CGAGATGTCT GACTTCATTG TGGACACGAG AGATGCAGGT 
TACGGAGGCA TCTCCTTGGC GGTGGAAGGC CCTAGCAAGG TGGACATCCA GACCGAGGAC 
CTGGAGGACG GCACCTGCAA GGTCTCCTAC TTCCCCACTG TGCCTGGGGT TTATATCGTC 
TCCACCAAGT TCGCTGACGA GCACGTGCCT GGGAGCCCGT TCACCGTGAA AATCAGCGGG 
GAGGGAAGAG TCAAGGAGAG CATCACCCGT ACCAGCCGAG CCCCGTCTGT GGCCACTGTT 
GGCAGCATCT GTGACCTGAA CCTGAAAATC CCAGAAATTG ACAGCAGTGA CATGTCAGCC 
CACGTCACCA GCCCCTCTGG CCGCGTGACC GAGGCGGAGA TCGTGCCGGT GGGGAAGAAC 
TCGCACTGCG TCCGGTTCGT GCCCCAGGAG ATGGGCGTTC ACACCGTCAG CGTCAAGTAC 
CGTGGCCAGC ACGTCACTGG CAGCCCCTTC CAGTTCACTG TGGGGCCGCT GGGCGAGGGG 
GGTGCCCACA AGGTCCGGGC AGGCGGCCCT GGCCTGGAGC GAGGAGAAGC CGGAGTTCCG 
GCGGAGTTCA GCATCTGGAC CCGGGAGGCA GGCGCCGGGG GCCTCTCCAT CGCCGTGGAG 
GGCCCCAGTA AGGCCGAGAT TACATTCGAT GACCATAAAA ACGGATCTTG TGGTGTGTCT 
TATATTGCCC AGGAACCTGG TAACTACGAG GTCTCCATCA AGTTCAATGA CGAGCATATT 
CCCGAAAGTC CCTATGTGGT GCCGGTGATC GCGCCCTCGG ACGACGCCCG CCGCCTCACT 
GTTCTGAGCC TTCAGGAATC GGGATTAAAA GTTAATCAGC CAGCCTCCTT TGCTATAAGG 
TTGAACGGGG CAAAAGGCAA GATCGATGCA AAGGTGCACA GCCCCTCTGG AGCCGTGGAG 
GAATGCCACG TGTCTGAGCT GGAGCCAGAT AAGTATGCTG TTCGCTTCAT CCCCCACGAG 
AATGGTATCC ACACGATTGA CGTCAAGTTC AACGGGAGCC ACGTGGTTGG AAGTCCCTTC 
AAAGTGCGCG TCGGGGAGCC TGGACAAGCG GGGAACCCCG CCCTGGTGTC GGCCTATGGC 
GCAGGGCTTG AGGGAGGCTC CACAGGTATC CAGTCAGAAT TTTTCATCAA TACCACCCGA 
GCAGGGCCAG GGACATTATC CGTCACCATC GAAGGGCCGT CCAAGGTTAA AATGGATTGC 
CAGGAAACCC CCGAAGGGTA CAAGGTCATG TACACCCCCA TGGCTCCTGG AAACTACCTG 
ATTGGCGTCA AATATGGCGG GCCCAACCAC ATCGTGGGCA GCCCGTTCAA GGCCAAGGTG 
ACAGGCCAGC GTCTGGTCAG CCCCGGCTCG GCCAACGAGA CCTCCTCCAT CCTGGTGGAG 
TCAGTGACCA GGTCCTCGAC GGAGACGTGC TACAGCGCCA TCCCCAAGGC ATCCTCAGAC 
GCCAGCAAGG TGACCTCCAA GGGCGCAGGG CTCTCGAAGG CCTTCCTGGG CCAGAAGAGC 
TCCTTCCTGG TGGACTGCAG CAAAGCCGGT TCCAACATGT TGCTGATCGG GGTCCACGGG 
CCCACCACCC CCTGCGAAGA AGTCTCCATG AAGCATGTGG GCAACCAGCA GTACAACGTG 
ACCTACGTCG TCAAGGAGCG GGGGGATTAC GTCCTGGCCG TGAAGTGGGG AGAGGAGCAC 
ATCCCCGGCA GTCCGTTCCA CGTCACCGTG CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_005222908.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222965.1 Bos taurus filamin B (FLNB), transcript variant X5, mRNA.

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CloneID	OBa229115
Clone ID Related Accession (Same CDS sequence)	XM_005222908.4
Accession Version	XM_005222908.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	filamin-B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222908.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA 
GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA 
GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG 
ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT 
GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG 
GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC 
ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA 
TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG 
GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC 
AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT 
GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG 
CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC 
ACTGTCATGG TAACAGAAGA GGCCTATGTC CCAGTGAGCA ACATGAACGG CCTGGGATTT 
AAGCCTTTCG ACTTGGTCAT TCCATTTGCA GTCAGGAAAG GAGAAATCAC CGGAGAGGTC 
CACATGCCTT CCGGGAAGAC GGCCAAGCCC GAGATTGTGG ACAACAAGGA CGGCACAGTC 
ACTGTCAGAT ACGCCCCCAC GGAGGTTGGC CTCCACGAGA TGCACATCAA GTACATGGGC 
AGCCACATTC CTGAGAGCCC CCTCCAGTTC TACGTGAACT ACCCCAACAG TGGGAGCGTG 
TCTGCGTACG GCCCAGGCCT GGTGTACGGA GTGGCCAACA AAACCGCCAC CTTCACCATC 
GTCACCGAAG ACGCCGGAGA AGGTGGCCTG GACTTGGCTA TCGAGGGACC CTCAAAGGCC 
GAAATCAGCT GTATTGACAA CAAAGATGGG ACGTGCACGG TGACTTACCT GCCCACCTTG 
CCGGGCGACT ACAGCATCCT GGTCAAGTAC AATGACAAAC ACATCCCTGG CAGCCCCTTC 
ACAGCCAAGA TCACAGACGA CAGCAGACGG TGCTCTCAGG TGAAGCTGGG CTCAGCCGCC 
GACTTCTTGC TTGACATCAG CGAGACTGAC CTCAGCACCT TGACGGCCAG CATCAAGGCC 
CCGTCCGGTC GTGACGAGCC TTGTCTCCTG AAGAGGCTGC CCAACAATCA CATCGGCATC 
TCCTTCATTC CCCGGGAGGT GGGTGAACAC CTGGTCAGCA TCAAGAAGAA CGGCAACCAC 
GTGGCCAACA GCCCCGTATC CATCATGGTG GTCCAGTCGG AGATTGGGGA TGCCCGCAGA 
GCCAAAGTCT ATGGCCGTGG CCTGTCAGAA GGCCGGACTT TCGAGATGTC TGACTTCATT 
GTGGACACGA GAGATGCAGG TTACGGAGGC ATCTCCTTGG CGGTGGAAGG CCCTAGCAAG 
GTGGACATCC AGACCGAGGA CCTGGAGGAC GGCACCTGCA AGGTCTCCTA CTTCCCCACT 
GTGCCTGGGG TTTATATCGT CTCCACCAAG TTCGCTGACG AGCACGTGCC TGGGAGCCCG 
TTCACCGTGA AAATCAGCGG GGAGGGAAGA GTCAAGGAGA GCATCACCCG TACCAGCCGA 
GCCCCGTCTG TGGCCACTGT TGGCAGCATC TGTGACCTGA ACCTGAAAAT CCCAGAAATT 
GACAGCAGTG ACATGTCAGC CCACGTCACC AGCCCCTCTG GCCGCGTGAC CGAGGCGGAG 
ATCGTGCCGG TGGGGAAGAA CTCGCACTGC GTCCGGTTCG TGCCCCAGGA GATGGGCGTT 
CACACCGTCA GCGTCAAGTA CCGTGGCCAG CACGTCACTG GCAGCCCCTT CCAGTTCACT 
GTGGGGCCGC TGGGCGAGGG GGGTGCCCAC AAGGTCCGGG CAGGCGGCCC TGGCCTGGAG 
CGAGGAGAAG CCGGAGTTCC GGCGGAGTTC AGCATCTGGA CCCGGGAGGC AGGCGCCGGG 
GGCCTCTCCA TCGCCGTGGA GGGCCCCAGT AAGGCCGAGA TTACATTCGA TGACCATAAA 
AACGGATCTT GTGGTGTGTC TTATATTGCC CAGGAACCTG GTAACTACGA GGTCTCCATC 
AAGTTCAATG ACGAGCATAT TCCCGAAAGT CCCTATGTGG TGCCGGTGAT CGCGCCCTCG 
GACGACGCCC GCCGCCTCAC TGTTCTGAGC CTTCAGGAAT CGGGATTAAA AGTTAATCAG 
CCAGCCTCCT TTGCTATAAG GTTGAACGGG GCAAAAGGCA AGATCGATGC AAAGGTGCAC 
AGCCCCTCTG GAGCCGTGGA GGAATGCCAC GTGTCTGAGC TGGAGCCAGA TAAGTATGCT 
GTTCGCTTCA TCCCCCACGA GAATGGTATC CACACGATTG ACGTCAAGTT CAACGGGAGC 
CACGTGGTTG GAAGTCCCTT CAAAGTGCGC GTCGGGGAGC CTGGACAAGC GGGGAACCCC 
GCCCTGGTGT CGGCCTATGG CGCAGGGCTT GAGGGAGGCT CCACAGGTAT CCAGTCAGAA 
TTTTTCATCA ATACCACCCG AGCAGGGCCA GGGACATTAT CCGTCACCAT CGAAGGGCCG 
TCCAAGGTTA AAATGGATTG CCAGGAAACC CCCGAAGGGT ACAAGGTCAT GTACACCCCC 
ATGGCTCCTG GAAACTACCT GATTGGCGTC AAATATGGCG GGCCCAACCA CATCGTGGGC 
AGCCCGTTCA AGGCCAAGGT GACAGGCCAG CGTCTGGTCA GCCCCGGCTC GGCCAACGAG 
ACCTCCTCCA TCCTGGTGGA GTCAGTGACC AGGTCCTCGA CGGAGACGTG CTACAGCGCC 
ATCCCCAAGG CATCCTCAGA CGCCAGCAAG GTGACCTCCA AGGGCGCAGG GCTCTCGAAG 
GCCTTCCTGG GCCAGAAGAG CTCCTTCCTG GTGGACTGCA GCAAAGCCGG TTCCAACATG 
TTGCTGATCG GGGTCCACGG GCCCACCACC CCCTGCGAAG AAGTCTCCAT GAAGCATGTG 
GGCAACCAGC AGTACAACGT GACCTACGTC GTCAAGGAGC GGGGGGATTA CGTCCTGGCC 
GTGAAGTGGG GAGAGGAGCA CATCCCCGGC AGTCCGTTCC ACGTCACCGT GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222965.1
CDS	178..7914
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKHMYRKYHQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPRKPVDNAREAMQQADDWLGVPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEALVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEVASNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDRGNQVYRCVYKPMQPGPHVVKVSFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGIRIRETADFKVDTKAAGSGELGVTIKGPKGLEELVKQKGFQDGVYAFEYYPSTQGKYSVSITWGGHHIQKSPFEVQVGPEAGMQKVRAWGPGLHGGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPFMAYIHPASGDYNPDLVQAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGDGQPIDIQMKSRMDGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVSIGQGSHPQKVKVFGPGVERSGLKAHEPTHFTVDCTEAGEGDVSVGIKCDARVLSDEEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPTSPFRVKVDPSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFSSPGPGDAVKDLDIIDNYDYSHTVKYTPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSRIKINGLENRVEVGKDQEFAVDTRGAGGQGKLDVTILSPSRKVVPCLVAPMAGREGSTAKFIPREEGLYAIDLTYDGHPVPGSPYVVEASLPPDPTKVKAHGPGLRGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDPSKVVASGPGLEHGKVGEPGLLSVDCSEAGPGTLGLEAVSDSGAKAEVCIENNKDGTYAVTYVPLTAGMYTLTMKYGGELVPHFPTRVKVEPAVDTSRVKVFGPGIEGKDVFREATTDFTVDSRPLTQVGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPVPNSPFKVAVTEGCQPSRVQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYVPFAPGDYDVNITYGGAHIPGSPFRVPVKDVVDPSKVKIVGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVMGPRGLVEPVNVVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSTYGVPASLPVEFAIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQTGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMVTEEAYVPVSNMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTAKPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEIDSSDMSAHVTSPSGRVTEAEIVPVGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAHKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYVVPVIAPSDDARRLTVLSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGAGLEGGSTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFLGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP

Target ORF information:

RefSeq Version	XM_005222908.4
Organism	Bos taurus(cattle)
Definition	Bos taurus filamin B (FLNB), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222908.4

ORF Insert Sequence:

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA 
GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA 
GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG 
ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT 
GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG 
GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC 
ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA 
TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG 
GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC 
AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT 
GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG 
CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC 
ACTGTCATGG TAACAGAAGA GGCCTATGTC CCAGTGAGCA ACATGAACGG CCTGGGATTT 
AAGCCTTTCG ACTTGGTCAT TCCATTTGCA GTCAGGAAAG GAGAAATCAC CGGAGAGGTC 
CACATGCCTT CCGGGAAGAC GGCCAAGCCC GAGATTGTGG ACAACAAGGA CGGCACAGTC 
ACTGTCAGAT ACGCCCCCAC GGAGGTTGGC CTCCACGAGA TGCACATCAA GTACATGGGC 
AGCCACATTC CTGAGAGCCC CCTCCAGTTC TACGTGAACT ACCCCAACAG TGGGAGCGTG 
TCTGCGTACG GCCCAGGCCT GGTGTACGGA GTGGCCAACA AAACCGCCAC CTTCACCATC 
GTCACCGAAG ACGCCGGAGA AGGTGGCCTG GACTTGGCTA TCGAGGGACC CTCAAAGGCC 
GAAATCAGCT GTATTGACAA CAAAGATGGG ACGTGCACGG TGACTTACCT GCCCACCTTG 
CCGGGCGACT ACAGCATCCT GGTCAAGTAC AATGACAAAC ACATCCCTGG CAGCCCCTTC 
ACAGCCAAGA TCACAGACGA CAGCAGACGG TGCTCTCAGG TGAAGCTGGG CTCAGCCGCC 
GACTTCTTGC TTGACATCAG CGAGACTGAC CTCAGCACCT TGACGGCCAG CATCAAGGCC 
CCGTCCGGTC GTGACGAGCC TTGTCTCCTG AAGAGGCTGC CCAACAATCA CATCGGCATC 
TCCTTCATTC CCCGGGAGGT GGGTGAACAC CTGGTCAGCA TCAAGAAGAA CGGCAACCAC 
GTGGCCAACA GCCCCGTATC CATCATGGTG GTCCAGTCGG AGATTGGGGA TGCCCGCAGA 
GCCAAAGTCT ATGGCCGTGG CCTGTCAGAA GGCCGGACTT TCGAGATGTC TGACTTCATT 
GTGGACACGA GAGATGCAGG TTACGGAGGC ATCTCCTTGG CGGTGGAAGG CCCTAGCAAG 
GTGGACATCC AGACCGAGGA CCTGGAGGAC GGCACCTGCA AGGTCTCCTA CTTCCCCACT 
GTGCCTGGGG TTTATATCGT CTCCACCAAG TTCGCTGACG AGCACGTGCC TGGGAGCCCG 
TTCACCGTGA AAATCAGCGG GGAGGGAAGA GTCAAGGAGA GCATCACCCG TACCAGCCGA 
GCCCCGTCTG TGGCCACTGT TGGCAGCATC TGTGACCTGA ACCTGAAAAT CCCAGAAATT 
GACAGCAGTG ACATGTCAGC CCACGTCACC AGCCCCTCTG GCCGCGTGAC CGAGGCGGAG 
ATCGTGCCGG TGGGGAAGAA CTCGCACTGC GTCCGGTTCG TGCCCCAGGA GATGGGCGTT 
CACACCGTCA GCGTCAAGTA CCGTGGCCAG CACGTCACTG GCAGCCCCTT CCAGTTCACT 
GTGGGGCCGC TGGGCGAGGG GGGTGCCCAC AAGGTCCGGG CAGGCGGCCC TGGCCTGGAG 
CGAGGAGAAG CCGGAGTTCC GGCGGAGTTC AGCATCTGGA CCCGGGAGGC AGGCGCCGGG 
GGCCTCTCCA TCGCCGTGGA GGGCCCCAGT AAGGCCGAGA TTACATTCGA TGACCATAAA 
AACGGATCTT GTGGTGTGTC TTATATTGCC CAGGAACCTG GTAACTACGA GGTCTCCATC 
AAGTTCAATG ACGAGCATAT TCCCGAAAGT CCCTATGTGG TGCCGGTGAT CGCGCCCTCG 
GACGACGCCC GCCGCCTCAC TGTTCTGAGC CTTCAGGAAT CGGGATTAAA AGTTAATCAG 
CCAGCCTCCT TTGCTATAAG GTTGAACGGG GCAAAAGGCA AGATCGATGC AAAGGTGCAC 
AGCCCCTCTG GAGCCGTGGA GGAATGCCAC GTGTCTGAGC TGGAGCCAGA TAAGTATGCT 
GTTCGCTTCA TCCCCCACGA GAATGGTATC CACACGATTG ACGTCAAGTT CAACGGGAGC 
CACGTGGTTG GAAGTCCCTT CAAAGTGCGC GTCGGGGAGC CTGGACAAGC GGGGAACCCC 
GCCCTGGTGT CGGCCTATGG CGCAGGGCTT GAGGGAGGCT CCACAGGTAT CCAGTCAGAA 
TTTTTCATCA ATACCACCCG AGCAGGGCCA GGGACATTAT CCGTCACCAT CGAAGGGCCG 
TCCAAGGTTA AAATGGATTG CCAGGAAACC CCCGAAGGGT ACAAGGTCAT GTACACCCCC 
ATGGCTCCTG GAAACTACCT GATTGGCGTC AAATATGGCG GGCCCAACCA CATCGTGGGC 
AGCCCGTTCA AGGCCAAGGT GACAGGCCAG CGTCTGGTCA GCCCCGGCTC GGCCAACGAG 
ACCTCCTCCA TCCTGGTGGA GTCAGTGACC AGGTCCTCGA CGGAGACGTG CTACAGCGCC 
ATCCCCAAGG CATCCTCAGA CGCCAGCAAG GTGACCTCCA AGGGCGCAGG GCTCTCGAAG 
GCCTTCCTGG GCCAGAAGAG CTCCTTCCTG GTGGACTGCA GCAAAGCCGG TTCCAACATG 
TTGCTGATCG GGGTCCACGG GCCCACCACC CCCTGCGAAG AAGTCTCCAT GAAGCATGTG 
GGCAACCAGC AGTACAACGT GACCTACGTC GTCAAGGAGC GGGGGGATTA CGTCCTGGCC 
GTGAAGTGGG GAGAGGAGCA CATCCCCGGC AGTCCGTTCC ACGTCACCGT GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( NM_001191460.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178389.1 Bos taurus filamin B (FLNB), mRNA.

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CloneID	OBa40688
Clone ID Related Accession (Same CDS sequence)	NM_001191460.3 , NM_001191460.1
Accession Version	NM_001191460.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	filamin-B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000022.1. On May 30, 2018 this sequence version replaced NM_001191460.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA 
GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG 
AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC 
CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC 
AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC 
TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA 
GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC 
GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG 
ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA 
CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA 
ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT 
TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC 
GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC 
TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC 
GCAGCTGTGA AGGAAGTACC GGTAAATGCA TGTCCCCCTG GATTCAGGCC CTGGGTAACA 
GAAGAGGCCT ATGTCCCAGT GAGCAACATG AACGGCCTGG GATTTAAGCC TTTCGACTTG 
GTCATTCCAT TTGCAGTCAG GAAAGGAGAA ATCACCGGAG AGGTCCACAT GCCTTCCGGG 
AAGACGGCCA AGCCCGAGAT TGTGGACAAC AAGGACGGCA CAGTCACTGT CAGATACGCC 
CCCACGGAGG TTGGCCTCCA CGAGATGCAC ATCAAGTACA TGGGCAGCCA CATTCCTGAG 
AGCCCCCTCC AGTTCTACGT GAACTACCCC AACAGTGGGA GCGTGTCTGC GTACGGCCCA 
GGCCTGGTGT ACGGAGTGGC CAACAAAACC GCCACCTTCA CCATCGTCAC CGAAGACGCC 
GGAGAAGGTG GCCTGGACTT GGCTATCGAG GGACCCTCAA AGGCCGAAAT CAGCTGTATT 
GACAACAAAG ATGGGACGTG CACGGTGACT TACCTGCCCA CCTTGCCGGG CGACTACAGC 
ATCCTGGTCA AGTACAATGA CAAACACATC CCTGGCAGCC CCTTCACAGC CAAGATCACA 
GACGACAGCA GACGGTGCTC TCAGGTGAAG CTGGGCTCAG CCGCCGACTT CTTGCTTGAC 
ATCAGCGAGA CTGACCTCAG CACCTTGACG GCCAGCATCA AGGCCCCGTC CGGTCGTGAC 
GAGCCTTGTC TCCTGAAGAG GCTGCCCAAC AATCACATCG GCATCTCCTT CATTCCCCGG 
GAGGTGGGTG AACACCTGGT CAGCATCAAG AAGAACGGCA ACCACGTGGC CAACAGCCCC 
GTATCCATCA TGGTGGTCCA GTCGGAGATT GGGGATGCCC GCAGAGCCAA AGTCTATGGC 
CGTGGCCTGT CAGAAGGCCG GACTTTCGAG ATGTCTGACT TCATTGTGGA CACGAGAGAT 
GCAGGTTACG GAGGCATCTC CTTGGCGGTG GAAGGCCCTA GCAAGGTGGA CATCCAGACC 
GAGGACCTGG AGGACGGCAC CTGCAAGGTC TCCTACTTCC CCACTGTGCC TGGGGTTTAT 
ATCGTCTCCA CCAAGTTCGC TGACGAGCAC GTGCCTGGGA GCCCGTTCAC CGTGAAAATC 
AGCGGGGAGG GAAGAGTCAA GGAGAGCATC ACCCGTACCA GCCGAGCCCC GTCTGTGGCC 
ACTGTTGGCA GCATCTGTGA CCTGAACCTG AAAATCCCAG AAATTGACAG CAGTGACATG 
TCAGCCCACG TCACCAGCCC CTCTGGCCGC GTGACCGAGG CGGAGATCGT GCCGGTGGGG 
AAGAACTCGC ACTGCGTCCG GTTCGTGCCC CAGGAGATGG GCGTTCACAC CGTCAGCGTC 
AAGTACCGTG GCCAGCACGT CACTGGCAGC CCCTTCCAGT TCACTGTGGG GCCGCTGGGC 
GAGGGGGGTG CCCACAAGGT CCGGGCAGGC GGCCCTGGCC TGGAGCGAGG AGAAGCCGGA 
GTTCCGGCGG AGTTCAGCAT CTGGACCCGG GAGGCAGGCG CCGGGGGCCT CTCCATCGCC 
GTGGAGGGCC CCAGTAAGGC CGAGATTACA TTCGATGACC ATAAAAACGG ATCTTGTGGT 
GTGTCTTATA TTGCCCAGGA ACCTGGTAAC TACGAGGTCT CCATCAAGTT CAATGACGAG 
CATATTCCCG AAAGTCCCTA TGTGGTGCCG GTGATCGCGC CCTCGGACGA CGCCCGCCGC 
CTCACTGTTC TGAGCCTTCA GGAATCGGGA TTAAAAGTTA ATCAGCCAGC CTCCTTTGCT 
ATAAGGTTGA ACGGGGCAAA AGGCAAGATC GATGCAAAGG TGCACAGCCC CTCTGGAGCC 
GTGGAGGAAT GCCACGTGTC TGAGCTGGAG CCAGATAAGT ATGCTGTTCG CTTCATCCCC 
CACGAGAATG GTATCCACAC GATTGACGTC AAGTTCAACG GGAGCCACGT GGTTGGAAGT 
CCCTTCAAAG TGCGCGTCGG GGAGCCTGGA CAAGCGGGGA ACCCCGCCCT GGTGTCGGCC 
TATGGCGCAG GGCTTGAGGG AGGCTCCACA GGTATCCAGT CAGAATTTTT CATCAATACC 
ACCCGAGCAG GGCCAGGGAC ATTATCCGTC ACCATCGAAG GGCCGTCCAA GGTTAAAATG 
GATTGCCAGG AAACCCCCGA AGGGTACAAG GTCATGTACA CCCCCATGGC TCCTGGAAAC 
TACCTGATTG GCGTCAAATA TGGCGGGCCC AACCACATCG TGGGCAGCCC GTTCAAGGCC 
AAGGTGACAG GCCAGCGTCT GGTCAGCCCC GGCTCGGCCA ACGAGACCTC CTCCATCCTG 
GTGGAGTCAG TGACCAGGTC CTCGACGGAG ACGTGCTACA GCGCCATCCC CAAGGCATCC 
TCAGACGCCA GCAAGGTGAC CTCCAAGGGC GCAGGGCTCT CGAAGGCCTT CCTGGGCCAG 
AAGAGCTCCT TCCTGGTGGA CTGCAGCAAA GCCGGTTCCA ACATGTTGCT GATCGGGGTC 
CACGGGCCCA CCACCCCCTG CGAAGAAGTC TCCATGAAGC ATGTGGGCAA CCAGCAGTAC 
AACGTGACCT ACGTCGTCAA GGAGCGGGGG GATTACGTCC TGGCCGTGAA GTGGGGAGAG 
GAGCACATCC CCGGCAGTCC GTTCCACGTC ACCGTGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178389.1
CDS	133..8034
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKHMYRKYHQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPRKPVDNAREAMQQADDWLGVPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEALVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEVASNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDRGNQVYRCVYKPMQPGPHVVKVSFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGIRIRETADFKVDTKAAGSGELGVTIKGPKGLEELVKQKGFQDGVYAFEYYPSTQGKYSVSITWGGHHIQKSPFEVQVGPEAGMQKVRAWGPGLHGGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPFMAYIHPASGDYNPDLVQAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGDGQPIDIQMKSRMDGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVSIGQGSHPQKVKVFGPGVERSGLKAHEPTHFTVDCTEAGEGDVSVGIKCDARVLSDEEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPTSPFRVKVDPSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFSSPGPGDAVKDLDIIDNYDYSHTVKYTPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSRIKINGLENRVEVGKDQEFAVDTRGAGGQGKLDVTILSPSRKVVPCLVAPMAGREGSTAKFIPREEGLYAIDLTYDGHPVPGSPYVVEASLPPDPTKVKAHGPGLRGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDPSKVVASGPGLEHGKVGEPGLLSVDCSEAGPGTLGLEAVSDSGAKAEVCIENNKDGTYAVTYVPLTAGMYTLTMKYGGELVPHFPTRVKVEPAVDTSRVKVFGPGIEGKDVFREATTDFTVDSRPLTQVGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPVPNSPFKVAVTEGCQPSRVQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYVPFAPGDYDVNITYGGAHIPGSPFRVPVKDVVDPSKVKIVGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVMGPRADQTISWSRRSPLKAISGFFKRDRKGDFMETGLVEPVNVVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSTYGVPASLPVEFAIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQTGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMATDGEVAAVKEVPVNACPPGFRPWVTEEAYVPVSNMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTAKPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEIDSSDMSAHVTSPSGRVTEAEIVPVGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAHKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYVVPVIAPSDDARRLTVLSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGAGLEGGSTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFLGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP

Target ORF information:

RefSeq Version	NM_001191460.3
Organism	Bos taurus(cattle)
Definition	Bos taurus filamin B (FLNB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191460.3

ORF Insert Sequence:

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA 
GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG 
AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC 
CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC 
AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC 
TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA 
GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC 
GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG 
ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA 
CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA 
ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT 
TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC 
GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC 
TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC 
GCAGCTGTGA AGGAAGTACC GGTAAATGCA TGTCCCCCTG GATTCAGGCC CTGGGTAACA 
GAAGAGGCCT ATGTCCCAGT GAGCAACATG AACGGCCTGG GATTTAAGCC TTTCGACTTG 
GTCATTCCAT TTGCAGTCAG GAAAGGAGAA ATCACCGGAG AGGTCCACAT GCCTTCCGGG 
AAGACGGCCA AGCCCGAGAT TGTGGACAAC AAGGACGGCA CAGTCACTGT CAGATACGCC 
CCCACGGAGG TTGGCCTCCA CGAGATGCAC ATCAAGTACA TGGGCAGCCA CATTCCTGAG 
AGCCCCCTCC AGTTCTACGT GAACTACCCC AACAGTGGGA GCGTGTCTGC GTACGGCCCA 
GGCCTGGTGT ACGGAGTGGC CAACAAAACC GCCACCTTCA CCATCGTCAC CGAAGACGCC 
GGAGAAGGTG GCCTGGACTT GGCTATCGAG GGACCCTCAA AGGCCGAAAT CAGCTGTATT 
GACAACAAAG ATGGGACGTG CACGGTGACT TACCTGCCCA CCTTGCCGGG CGACTACAGC 
ATCCTGGTCA AGTACAATGA CAAACACATC CCTGGCAGCC CCTTCACAGC CAAGATCACA 
GACGACAGCA GACGGTGCTC TCAGGTGAAG CTGGGCTCAG CCGCCGACTT CTTGCTTGAC 
ATCAGCGAGA CTGACCTCAG CACCTTGACG GCCAGCATCA AGGCCCCGTC CGGTCGTGAC 
GAGCCTTGTC TCCTGAAGAG GCTGCCCAAC AATCACATCG GCATCTCCTT CATTCCCCGG 
GAGGTGGGTG AACACCTGGT CAGCATCAAG AAGAACGGCA ACCACGTGGC CAACAGCCCC 
GTATCCATCA TGGTGGTCCA GTCGGAGATT GGGGATGCCC GCAGAGCCAA AGTCTATGGC 
CGTGGCCTGT CAGAAGGCCG GACTTTCGAG ATGTCTGACT TCATTGTGGA CACGAGAGAT 
GCAGGTTACG GAGGCATCTC CTTGGCGGTG GAAGGCCCTA GCAAGGTGGA CATCCAGACC 
GAGGACCTGG AGGACGGCAC CTGCAAGGTC TCCTACTTCC CCACTGTGCC TGGGGTTTAT 
ATCGTCTCCA CCAAGTTCGC TGACGAGCAC GTGCCTGGGA GCCCGTTCAC CGTGAAAATC 
AGCGGGGAGG GAAGAGTCAA GGAGAGCATC ACCCGTACCA GCCGAGCCCC GTCTGTGGCC 
ACTGTTGGCA GCATCTGTGA CCTGAACCTG AAAATCCCAG AAATTGACAG CAGTGACATG 
TCAGCCCACG TCACCAGCCC CTCTGGCCGC GTGACCGAGG CGGAGATCGT GCCGGTGGGG 
AAGAACTCGC ACTGCGTCCG GTTCGTGCCC CAGGAGATGG GCGTTCACAC CGTCAGCGTC 
AAGTACCGTG GCCAGCACGT CACTGGCAGC CCCTTCCAGT TCACTGTGGG GCCGCTGGGC 
GAGGGGGGTG CCCACAAGGT CCGGGCAGGC GGCCCTGGCC TGGAGCGAGG AGAAGCCGGA 
GTTCCGGCGG AGTTCAGCAT CTGGACCCGG GAGGCAGGCG CCGGGGGCCT CTCCATCGCC 
GTGGAGGGCC CCAGTAAGGC CGAGATTACA TTCGATGACC ATAAAAACGG ATCTTGTGGT 
GTGTCTTATA TTGCCCAGGA ACCTGGTAAC TACGAGGTCT CCATCAAGTT CAATGACGAG 
CATATTCCCG AAAGTCCCTA TGTGGTGCCG GTGATCGCGC CCTCGGACGA CGCCCGCCGC 
CTCACTGTTC TGAGCCTTCA GGAATCGGGA TTAAAAGTTA ATCAGCCAGC CTCCTTTGCT 
ATAAGGTTGA ACGGGGCAAA AGGCAAGATC GATGCAAAGG TGCACAGCCC CTCTGGAGCC 
GTGGAGGAAT GCCACGTGTC TGAGCTGGAG CCAGATAAGT ATGCTGTTCG CTTCATCCCC 
CACGAGAATG GTATCCACAC GATTGACGTC AAGTTCAACG GGAGCCACGT GGTTGGAAGT 
CCCTTCAAAG TGCGCGTCGG GGAGCCTGGA CAAGCGGGGA ACCCCGCCCT GGTGTCGGCC 
TATGGCGCAG GGCTTGAGGG AGGCTCCACA GGTATCCAGT CAGAATTTTT CATCAATACC 
ACCCGAGCAG GGCCAGGGAC ATTATCCGTC ACCATCGAAG GGCCGTCCAA GGTTAAAATG 
GATTGCCAGG AAACCCCCGA AGGGTACAAG GTCATGTACA CCCCCATGGC TCCTGGAAAC 
TACCTGATTG GCGTCAAATA TGGCGGGCCC AACCACATCG TGGGCAGCCC GTTCAAGGCC 
AAGGTGACAG GCCAGCGTCT GGTCAGCCCC GGCTCGGCCA ACGAGACCTC CTCCATCCTG 
GTGGAGTCAG TGACCAGGTC CTCGACGGAG ACGTGCTACA GCGCCATCCC CAAGGCATCC 
TCAGACGCCA GCAAGGTGAC CTCCAAGGGC GCAGGGCTCT CGAAGGCCTT CCTGGGCCAG 
AAGAGCTCCT TCCTGGTGGA CTGCAGCAAA GCCGGTTCCA ACATGTTGCT GATCGGGGTC 
CACGGGCCCA CCACCCCCTG CGAAGAAGTC TCCATGAAGC ATGTGGGCAA CCAGCAGTAC 
AACGTGACCT ACGTCGTCAA GGAGCGGGGG GATTACGTCC TGGCCGTGAA GTGGGGAGAG 
GAGCACATCC CCGGCAGTCC GTTCCACGTC ACCGTGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( NM_001191460.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178389.1 Bos taurus filamin B, beta (FLNB), mRNA.

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CloneID	OBa40688
Clone ID Related Accession (Same CDS sequence)	NM_001191460.3 , NM_001191460.1
Accession Version	NM_001191460.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	filamin-B
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03009325.1, AAFC03128276.1, AAFC03009324.1 and AAFC03007718.1. On Jul 17, 2010 this sequence version replaced XM_002702763.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA 
GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG 
AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC 
CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC 
AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC 
TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA 
GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC 
GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG 
ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA 
CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA 
ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT 
TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC 
GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC 
TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC 
GCAGCTGTGA AGGAAGTACC GGTAAATGCA TGTCCCCCTG GATTCAGGCC CTGGGTAACA 
GAAGAGGCCT ATGTCCCAGT GAGCAACATG AACGGCCTGG GATTTAAGCC TTTCGACTTG 
GTCATTCCAT TTGCAGTCAG GAAAGGAGAA ATCACCGGAG AGGTCCACAT GCCTTCCGGG 
AAGACGGCCA AGCCCGAGAT TGTGGACAAC AAGGACGGCA CAGTCACTGT CAGATACGCC 
CCCACGGAGG TTGGCCTCCA CGAGATGCAC ATCAAGTACA TGGGCAGCCA CATTCCTGAG 
AGCCCCCTCC AGTTCTACGT GAACTACCCC AACAGTGGGA GCGTGTCTGC GTACGGCCCA 
GGCCTGGTGT ACGGAGTGGC CAACAAAACC GCCACCTTCA CCATCGTCAC CGAAGACGCC 
GGAGAAGGTG GCCTGGACTT GGCTATCGAG GGACCCTCAA AGGCCGAAAT CAGCTGTATT 
GACAACAAAG ATGGGACGTG CACGGTGACT TACCTGCCCA CCTTGCCGGG CGACTACAGC 
ATCCTGGTCA AGTACAATGA CAAACACATC CCTGGCAGCC CCTTCACAGC CAAGATCACA 
GACGACAGCA GACGGTGCTC TCAGGTGAAG CTGGGCTCAG CCGCCGACTT CTTGCTTGAC 
ATCAGCGAGA CTGACCTCAG CACCTTGACG GCCAGCATCA AGGCCCCGTC CGGTCGTGAC 
GAGCCTTGTC TCCTGAAGAG GCTGCCCAAC AATCACATCG GCATCTCCTT CATTCCCCGG 
GAGGTGGGTG AACACCTGGT CAGCATCAAG AAGAACGGCA ACCACGTGGC CAACAGCCCC 
GTATCCATCA TGGTGGTCCA GTCGGAGATT GGGGATGCCC GCAGAGCCAA AGTCTATGGC 
CGTGGCCTGT CAGAAGGCCG GACTTTCGAG ATGTCTGACT TCATTGTGGA CACGAGAGAT 
GCAGGTTACG GAGGCATCTC CTTGGCGGTG GAAGGCCCTA GCAAGGTGGA CATCCAGACC 
GAGGACCTGG AGGACGGCAC CTGCAAGGTC TCCTACTTCC CCACTGTGCC TGGGGTTTAT 
ATCGTCTCCA CCAAGTTCGC TGACGAGCAC GTGCCTGGGA GCCCGTTCAC CGTGAAAATC 
AGCGGGGAGG GAAGAGTCAA GGAGAGCATC ACCCGTACCA GCCGAGCCCC GTCTGTGGCC 
ACTGTTGGCA GCATCTGTGA CCTGAACCTG AAAATCCCAG AAATTGACAG CAGTGACATG 
TCAGCCCACG TCACCAGCCC CTCTGGCCGC GTGACCGAGG CGGAGATCGT GCCGGTGGGG 
AAGAACTCGC ACTGCGTCCG GTTCGTGCCC CAGGAGATGG GCGTTCACAC CGTCAGCGTC 
AAGTACCGTG GCCAGCACGT CACTGGCAGC CCCTTCCAGT TCACTGTGGG GCCGCTGGGC 
GAGGGGGGTG CCCACAAGGT CCGGGCAGGC GGCCCTGGCC TGGAGCGAGG AGAAGCCGGA 
GTTCCGGCGG AGTTCAGCAT CTGGACCCGG GAGGCAGGCG CCGGGGGCCT CTCCATCGCC 
GTGGAGGGCC CCAGTAAGGC CGAGATTACA TTCGATGACC ATAAAAACGG ATCTTGTGGT 
GTGTCTTATA TTGCCCAGGA ACCTGGTAAC TACGAGGTCT CCATCAAGTT CAATGACGAG 
CATATTCCCG AAAGTCCCTA TGTGGTGCCG GTGATCGCGC CCTCGGACGA CGCCCGCCGC 
CTCACTGTTC TGAGCCTTCA GGAATCGGGA TTAAAAGTTA ATCAGCCAGC CTCCTTTGCT 
ATAAGGTTGA ACGGGGCAAA AGGCAAGATC GATGCAAAGG TGCACAGCCC CTCTGGAGCC 
GTGGAGGAAT GCCACGTGTC TGAGCTGGAG CCAGATAAGT ATGCTGTTCG CTTCATCCCC 
CACGAGAATG GTATCCACAC GATTGACGTC AAGTTCAACG GGAGCCACGT GGTTGGAAGT 
CCCTTCAAAG TGCGCGTCGG GGAGCCTGGA CAAGCGGGGA ACCCCGCCCT GGTGTCGGCC 
TATGGCGCAG GGCTTGAGGG AGGCTCCACA GGTATCCAGT CAGAATTTTT CATCAATACC 
ACCCGAGCAG GGCCAGGGAC ATTATCCGTC ACCATCGAAG GGCCGTCCAA GGTTAAAATG 
GATTGCCAGG AAACCCCCGA AGGGTACAAG GTCATGTACA CCCCCATGGC TCCTGGAAAC 
TACCTGATTG GCGTCAAATA TGGCGGGCCC AACCACATCG TGGGCAGCCC GTTCAAGGCC 
AAGGTGACAG GCCAGCGTCT GGTCAGCCCC GGCTCGGCCA ACGAGACCTC CTCCATCCTG 
GTGGAGTCAG TGACCAGGTC CTCGACGGAG ACGTGCTACA GCGCCATCCC CAAGGCATCC 
TCAGACGCCA GCAAGGTGAC CTCCAAGGGC GCAGGGCTCT CGAAGGCCTT CCTGGGCCAG 
AAGAGCTCCT TCCTGGTGGA CTGCAGCAAA GCCGGTTCCA ACATGTTGCT GATCGGGGTC 
CACGGGCCCA CCACCCCCTG CGAAGAAGTC TCCATGAAGC ATGTGGGCAA CCAGCAGTAC 
AACGTGACCT ACGTCGTCAA GGAGCGGGGG GATTACGTCC TGGCCGTGAA GTGGGGAGAG 
GAGCACATCC CCGGCAGTCC GTTCCACGTC ACCGTGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178389.1
CDS	133..8034
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKHMYRKYHQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPRKPVDNAREAMQQADDWLGVPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEALVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEVASNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDRGNQVYRCVYKPMQPGPHVVKVSFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGIRIRETADFKVDTKAAGSGELGVTIKGPKGLEELVKQKGFQDGVYAFEYYPSTQGKYSVSITWGGHHIQKSPFEVQVGPEAGMQKVRAWGPGLHGGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPFMAYIHPASGDYNPDLVQAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGDGQPIDIQMKSRMDGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVSIGQGSHPQKVKVFGPGVERSGLKAHEPTHFTVDCTEAGEGDVSVGIKCDARVLSDEEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPTSPFRVKVDPSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFSSPGPGDAVKDLDIIDNYDYSHTVKYTPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSRIKINGLENRVEVGKDQEFAVDTRGAGGQGKLDVTILSPSRKVVPCLVAPMAGREGSTAKFIPREEGLYAIDLTYDGHPVPGSPYVVEASLPPDPTKVKAHGPGLRGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDPSKVVASGPGLEHGKVGEPGLLSVDCSEAGPGTLGLEAVSDSGAKAEVCIENNKDGTYAVTYVPLTAGMYTLTMKYGGELVPHFPTRVKVEPAVDTSRVKVFGPGIEGKDVFREATTDFTVDSRPLTQVGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPVPNSPFKVAVTEGCQPSRVQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYVPFAPGDYDVNITYGGAHIPGSPFRVPVKDVVDPSKVKIVGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVMGPRADQTISWSRRSPLKAISGFFKRDRKGDFMETGLVEPVNVVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSTYGVPASLPVEFAIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQTGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMATDGEVAAVKEVPVNACPPGFRPWVTEEAYVPVSNMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTAKPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEIDSSDMSAHVTSPSGRVTEAEIVPVGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAHKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYVVPVIAPSDDARRLTVLSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGAGLEGGSTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFLGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP

Target ORF information:

RefSeq Version	NM_001191460.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus filamin B, beta (FLNB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191460.1

ORF Insert Sequence:

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCGCACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA 
GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG 
AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC 
CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC 
AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC 
TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA 
GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC 
GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG 
ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA 
CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA 
ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT 
TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC 
GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC 
TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC 
GCAGCTGTGA AGGAAGTACC GGTAAATGCA TGTCCCCCTG GATTCAGGCC CTGGGTAACA 
GAAGAGGCCT ATGTCCCAGT GAGCAACATG AACGGCCTGG GATTTAAGCC TTTCGACTTG 
GTCATTCCAT TTGCAGTCAG GAAAGGAGAA ATCACCGGAG AGGTCCACAT GCCTTCCGGG 
AAGACGGCCA AGCCCGAGAT TGTGGACAAC AAGGACGGCA CAGTCACTGT CAGATACGCC 
CCCACGGAGG TTGGCCTCCA CGAGATGCAC ATCAAGTACA TGGGCAGCCA CATTCCTGAG 
AGCCCCCTCC AGTTCTACGT GAACTACCCC AACAGTGGGA GCGTGTCTGC GTACGGCCCA 
GGCCTGGTGT ACGGAGTGGC CAACAAAACC GCCACCTTCA CCATCGTCAC CGAAGACGCC 
GGAGAAGGTG GCCTGGACTT GGCTATCGAG GGACCCTCAA AGGCCGAAAT CAGCTGTATT 
GACAACAAAG ATGGGACGTG CACGGTGACT TACCTGCCCA CCTTGCCGGG CGACTACAGC 
ATCCTGGTCA AGTACAATGA CAAACACATC CCTGGCAGCC CCTTCACAGC CAAGATCACA 
GACGACAGCA GACGGTGCTC TCAGGTGAAG CTGGGCTCAG CCGCCGACTT CTTGCTTGAC 
ATCAGCGAGA CTGACCTCAG CACCTTGACG GCCAGCATCA AGGCCCCGTC CGGTCGTGAC 
GAGCCTTGTC TCCTGAAGAG GCTGCCCAAC AATCACATCG GCATCTCCTT CATTCCCCGG 
GAGGTGGGTG AACACCTGGT CAGCATCAAG AAGAACGGCA ACCACGTGGC CAACAGCCCC 
GTATCCATCA TGGTGGTCCA GTCGGAGATT GGGGATGCCC GCAGAGCCAA AGTCTATGGC 
CGTGGCCTGT CAGAAGGCCG GACTTTCGAG ATGTCTGACT TCATTGTGGA CACGAGAGAT 
GCAGGTTACG GAGGCATCTC CTTGGCGGTG GAAGGCCCTA GCAAGGTGGA CATCCAGACC 
GAGGACCTGG AGGACGGCAC CTGCAAGGTC TCCTACTTCC CCACTGTGCC TGGGGTTTAT 
ATCGTCTCCA CCAAGTTCGC TGACGAGCAC GTGCCTGGGA GCCCGTTCAC CGTGAAAATC 
AGCGGGGAGG GAAGAGTCAA GGAGAGCATC ACCCGTACCA GCCGAGCCCC GTCTGTGGCC 
ACTGTTGGCA GCATCTGTGA CCTGAACCTG AAAATCCCAG AAATTGACAG CAGTGACATG 
TCAGCCCACG TCACCAGCCC CTCTGGCCGC GTGACCGAGG CGGAGATCGT GCCGGTGGGG 
AAGAACTCGC ACTGCGTCCG GTTCGTGCCC CAGGAGATGG GCGTTCACAC CGTCAGCGTC 
AAGTACCGTG GCCAGCACGT CACTGGCAGC CCCTTCCAGT TCACTGTGGG GCCGCTGGGC 
GAGGGGGGTG CCCACAAGGT CCGGGCAGGC GGCCCTGGCC TGGAGCGAGG AGAAGCCGGA 
GTTCCGGCGG AGTTCAGCAT CTGGACCCGG GAGGCAGGCG CCGGGGGCCT CTCCATCGCC 
GTGGAGGGCC CCAGTAAGGC CGAGATTACA TTCGATGACC ATAAAAACGG ATCTTGTGGT 
GTGTCTTATA TTGCCCAGGA ACCTGGTAAC TACGAGGTCT CCATCAAGTT CAATGACGAG 
CATATTCCCG AAAGTCCCTA TGTGGTGCCG GTGATCGCGC CCTCGGACGA CGCCCGCCGC 
CTCACTGTTC TGAGCCTTCA GGAATCGGGA TTAAAAGTTA ATCAGCCAGC CTCCTTTGCT 
ATAAGGTTGA ACGGGGCAAA AGGCAAGATC GATGCAAAGG TGCACAGCCC CTCTGGAGCC 
GTGGAGGAAT GCCACGTGTC TGAGCTGGAG CCAGATAAGT ATGCTGTTCG CTTCATCCCC 
CACGAGAATG GTATCCACAC GATTGACGTC AAGTTCAACG GGAGCCACGT GGTTGGAAGT 
CCCTTCAAAG TGCGCGTCGG GGAGCCTGGA CAAGCGGGGA ACCCCGCCCT GGTGTCGGCC 
TATGGCGCAG GGCTTGAGGG AGGCTCCACA GGTATCCAGT CAGAATTTTT CATCAATACC 
ACCCGAGCAG GGCCAGGGAC ATTATCCGTC ACCATCGAAG GGCCGTCCAA GGTTAAAATG 
GATTGCCAGG AAACCCCCGA AGGGTACAAG GTCATGTACA CCCCCATGGC TCCTGGAAAC 
TACCTGATTG GCGTCAAATA TGGCGGGCCC AACCACATCG TGGGCAGCCC GTTCAAGGCC 
AAGGTGACAG GCCAGCGTCT GGTCAGCCCC GGCTCGGCCA ACGAGACCTC CTCCATCCTG 
GTGGAGTCAG TGACCAGGTC CTCGACGGAG ACGTGCTACA GCGCCATCCC CAAGGCATCC 
TCAGACGCCA GCAAGGTGAC CTCCAAGGGC GCAGGGCTCT CGAAGGCCTT CCTGGGCCAG 
AAGAGCTCCT TCCTGGTGGA CTGCAGCAAA GCCGGTTCCA ACATGTTGCT GATCGGGGTC 
CACGGGCCCA CCACCCCCTG CGAAGAAGTC TCCATGAAGC ATGTGGGCAA CCAGCAGTAC 
AACGTGACCT ACGTCGTCAA GGAGCGGGGG GATTACGTCC TGGCCGTGAA GTGGGGAGAG 
GAGCACATCC CCGGCAGTCC GTTCCACGTC ACCGTGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_005222904.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222961.1 Bos taurus filamin B (FLNB), transcript variant X1, mRNA.

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CloneID	OBa31625
Clone ID Related Accession (Same CDS sequence)	XM_005222904.3
Accession Version	XM_005222904.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	filamin-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222904.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA 
GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG 
AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC 
CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC 
AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC 
TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA 
GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC 
GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG 
ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA 
CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA 
ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT 
TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC 
GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC 
TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC 
GCAGCTGTGA AGGAAGTACC GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC 
GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC 
ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG 
GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC 
AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC 
AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC 
ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA 
CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC 
CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT 
GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG 
GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC 
AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT 
CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG 
AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG 
GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG 
TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA 
GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC 
TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG 
CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC 
CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA 
ATCCCAGAAA TTGACAGCAG TGACATGTCG GCCCACGTCA CCAGCCCCTC TGGCCGCGTG 
ACCGAGGCAG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG 
GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC 
TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC 
CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG 
GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC 
GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC 
GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG 
ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA 
AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT 
GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA 
GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG 
TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA 
GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT 
ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC 
ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC 
ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC 
CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC 
TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG 
TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA 
GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC 
GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC 
ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT 
TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC 
GTGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222961.1
CDS	177..8045
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKHMYRKYHQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPRKPVDNAREAMQQADDWLGVPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEALVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEVASNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDRGNQVYRCVYKPMQPGPHVVKVSFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGIRIRETADFKVDTKAAGSGELGVTIKGPKGLEELVKQKGFQDGVYAFEYYPSTQGKYSVSITWGGHHIQKSPFEVQVGPEAGMQKVRAWGPGLHGGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPFMAYIHPASGDYNPDLVQAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGDGQPIDIQMKSRMDGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVSIGQGSHPQKVKVFGPGVERSGLKAHEPTHFTVDCTEAGEGDVSVGIKCDARVLSDEEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPTSPFRVKVDPSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFSSPGPGDAVKDLDIIDNYDYSHTVKYTPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSRIKINGLENRVEVGKDQEFAVDTRGAGGQGKLDVTILSPSRKVVPCLVAPMAGREGSTAKFIPREEGLYAIDLTYDGHPVPGSPYVVEASLPPDPTKVKAHGPGLRGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDPSKVVASGPGLEHGKVGEPGLLSVDCSEAGPGTLGLEAVSDSGAKAEVCIENNKDGTYAVTYVPLTAGMYTLTMKYGGELVPHFPTRVKVEPAVDTSRVKVFGPGIEGKDVFREATTDFTVDSRPLTQVGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPVPNSPFKVAVTEGCQPSRVQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYVPFAPGDYDVNITYGGAHIPGSPFRVPVKDVVDPSKVKIVGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVMGPRADQTISWSRRSPLKAISGFFKRDRKGDFMETGLVEPVNVVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSTYGVPASLPVEFAIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQTGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMATDGEVAAVKEVPVTEEAYVPVSNMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTAKPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEIDSSDMSAHVTSPSGRVTEAEIVPVGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAHKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYVVPVIAPSDDARRLTVLSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGAGLEGGSTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFLGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP

Target ORF information:

RefSeq Version	XM_005222904.3
Organism	Bos taurus(cattle)
Definition	Bos taurus filamin B (FLNB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222904.3

ORF Insert Sequence:

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA 
GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG 
AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC 
CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC 
AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC 
TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA 
GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC 
GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG 
ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA 
CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA 
ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT 
TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC 
GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC 
TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGCCACAGA TGGGGAAGTC 
GCAGCTGTGA AGGAAGTACC GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC 
GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC 
ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG 
GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC 
AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC 
AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC 
ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA 
CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC 
CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT 
GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG 
GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC 
AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT 
CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG 
AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG 
GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG 
TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA 
GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC 
TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG 
CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC 
CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA 
ATCCCAGAAA TTGACAGCAG TGACATGTCG GCCCACGTCA CCAGCCCCTC TGGCCGCGTG 
ACCGAGGCAG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG 
GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC 
TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC 
CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG 
GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC 
GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC 
GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG 
ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA 
AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT 
GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA 
GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG 
TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA 
GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT 
ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC 
ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC 
ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC 
CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC 
TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG 
TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA 
GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC 
GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC 
ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT 
TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC 
GTGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_005222905.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222962.1 Bos taurus filamin B (FLNB), transcript variant X2, mRNA.

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CloneID	OBa31626
Clone ID Related Accession (Same CDS sequence)	XM_005222905.3
Accession Version	XM_005222905.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	filamin-B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222905.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA 
GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG 
AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC 
CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC 
AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC 
TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA 
GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC 
GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG 
ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA 
CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA 
ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT 
TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC 
GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC 
TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGTAACAGA AGAGGCCTAT 
GTCCCAGTGA GCAACATGAA CGGCCTGGGA TTTAAGCCTT TCGACTTGGT CATTCCATTT 
GCAGTCAGGA AAGGAGAAAT CACCGGAGAG GTCCACATGC CTTCCGGGAA GACGGCCAAG 
CCCGAGATTG TGGACAACAA GGACGGCACA GTCACTGTCA GATACGCCCC CACGGAGGTT 
GGCCTCCACG AGATGCACAT CAAGTACATG GGCAGCCACA TTCCTGAGAG CCCCCTCCAG 
TTCTACGTGA ACTACCCCAA CAGTGGGAGC GTGTCTGCGT ACGGCCCAGG CCTGGTGTAC 
GGAGTGGCCA ACAAAACCGC CACCTTCACC ATCGTCACCG AAGACGCCGG AGAAGGTGGC 
CTGGACTTGG CTATCGAGGG ACCCTCAAAG GCCGAAATCA GCTGTATTGA CAACAAAGAT 
GGGACGTGCA CGGTGACTTA CCTGCCCACC TTGCCGGGCG ACTACAGCAT CCTGGTCAAG 
TACAATGACA AACACATCCC TGGCAGCCCC TTCACAGCCA AGATCACAGA CGACAGCAGA 
CGGTGCTCTC AGGTGAAGCT GGGCTCAGCC GCCGACTTCT TGCTTGACAT CAGCGAGACT 
GACCTCAGCA CCTTGACGGC CAGCATCAAG GCCCCGTCCG GTCGTGACGA GCCTTGTCTC 
CTGAAGAGGC TGCCCAACAA TCACATCGGC ATCTCCTTCA TTCCCCGGGA GGTGGGTGAA 
CACCTGGTCA GCATCAAGAA GAACGGCAAC CACGTGGCCA ACAGCCCCGT ATCCATCATG 
GTGGTCCAGT CGGAGATTGG GGATGCCCGC AGAGCCAAAG TCTATGGCCG TGGCCTGTCA 
GAAGGCCGGA CTTTCGAGAT GTCTGACTTC ATTGTGGACA CGAGAGATGC AGGTTACGGA 
GGCATCTCCT TGGCGGTGGA AGGCCCTAGC AAGGTGGACA TCCAGACCGA GGACCTGGAG 
GACGGCACCT GCAAGGTCTC CTACTTCCCC ACTGTGCCTG GGGTTTATAT CGTCTCCACC 
AAGTTCGCTG ACGAGCACGT GCCTGGGAGC CCGTTCACCG TGAAAATCAG CGGGGAGGGA 
AGAGTCAAGG AGAGCATCAC CCGTACCAGC CGAGCCCCGT CTGTGGCCAC TGTTGGCAGC 
ATCTGTGACC TGAACCTGAA AATCCCAGAA ATTGACAGCA GTGACATGTC GGCCCACGTC 
ACCAGCCCCT CTGGCCGCGT GACCGAGGCA GAGATCGTGC CGGTGGGGAA GAACTCGCAC 
TGCGTCCGGT TCGTGCCCCA GGAGATGGGC GTTCACACCG TCAGCGTCAA GTACCGTGGC 
CAGCACGTCA CTGGCAGCCC CTTCCAGTTC ACTGTGGGGC CGCTGGGCGA GGGGGGTGCC 
CACAAGGTCC GGGCAGGCGG CCCTGGCCTG GAGCGAGGAG AAGCCGGAGT TCCGGCGGAG 
TTCAGCATCT GGACCCGGGA GGCAGGCGCC GGGGGCCTCT CCATCGCCGT GGAGGGCCCC 
AGTAAGGCCG AGATTACATT CGATGACCAT AAAAACGGAT CTTGTGGTGT GTCTTATATT 
GCCCAGGAAC CTGGTAACTA CGAGGTCTCC ATCAAGTTCA ATGACGAGCA TATTCCCGAA 
AGTCCCTATG TGGTGCCGGT GATCGCGCCC TCGGACGACG CCCGCCGCCT CACTGTTCTG 
AGCCTTCAGG AATCGGGATT AAAAGTTAAT CAGCCAGCCT CCTTTGCTAT AAGGTTGAAC 
GGGGCAAAAG GCAAGATCGA TGCAAAGGTG CACAGCCCCT CTGGAGCCGT GGAGGAATGC 
CACGTGTCTG AGCTGGAGCC AGATAAGTAT GCTGTTCGCT TCATCCCCCA CGAGAATGGT 
ATCCACACGA TTGACGTCAA GTTCAACGGG AGCCACGTGG TTGGAAGTCC CTTCAAAGTG 
CGCGTCGGGG AGCCTGGACA AGCGGGGAAC CCCGCCCTGG TGTCGGCCTA TGGCGCAGGG 
CTTGAGGGAG GCTCCACAGG TATCCAGTCA GAATTTTTCA TCAATACCAC CCGAGCAGGG 
CCAGGGACAT TATCCGTCAC CATCGAAGGG CCGTCCAAGG TTAAAATGGA TTGCCAGGAA 
ACCCCCGAAG GGTACAAGGT CATGTACACC CCCATGGCTC CTGGAAACTA CCTGATTGGC 
GTCAAATATG GCGGGCCCAA CCACATCGTG GGCAGCCCGT TCAAGGCCAA GGTGACAGGC 
CAGCGTCTGG TCAGCCCCGG CTCGGCCAAC GAGACCTCCT CCATCCTGGT GGAGTCAGTG 
ACCAGGTCCT CGACGGAGAC GTGCTACAGC GCCATCCCCA AGGCATCCTC AGACGCCAGC 
AAGGTGACCT CCAAGGGCGC AGGGCTCTCG AAGGCCTTCC TGGGCCAGAA GAGCTCCTTC 
CTGGTGGACT GCAGCAAAGC CGGTTCCAAC ATGTTGCTGA TCGGGGTCCA CGGGCCCACC 
ACCCCCTGCG AAGAAGTCTC CATGAAGCAT GTGGGCAACC AGCAGTACAA CGTGACCTAC 
GTCGTCAAGG AGCGGGGGGA TTACGTCCTG GCCGTGAAGT GGGGAGAGGA GCACATCCCC 
GGCAGTCCGT TCCACGTCAC CGTGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222962.1
CDS	177..8006
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKHMYRKYHQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPRKPVDNAREAMQQADDWLGVPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEALVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEVASNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDRGNQVYRCVYKPMQPGPHVVKVSFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGIRIRETADFKVDTKAAGSGELGVTIKGPKGLEELVKQKGFQDGVYAFEYYPSTQGKYSVSITWGGHHIQKSPFEVQVGPEAGMQKVRAWGPGLHGGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPFMAYIHPASGDYNPDLVQAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGDGQPIDIQMKSRMDGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVSIGQGSHPQKVKVFGPGVERSGLKAHEPTHFTVDCTEAGEGDVSVGIKCDARVLSDEEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPTSPFRVKVDPSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFSSPGPGDAVKDLDIIDNYDYSHTVKYTPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSRIKINGLENRVEVGKDQEFAVDTRGAGGQGKLDVTILSPSRKVVPCLVAPMAGREGSTAKFIPREEGLYAIDLTYDGHPVPGSPYVVEASLPPDPTKVKAHGPGLRGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDPSKVVASGPGLEHGKVGEPGLLSVDCSEAGPGTLGLEAVSDSGAKAEVCIENNKDGTYAVTYVPLTAGMYTLTMKYGGELVPHFPTRVKVEPAVDTSRVKVFGPGIEGKDVFREATTDFTVDSRPLTQVGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPVPNSPFKVAVTEGCQPSRVQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYVPFAPGDYDVNITYGGAHIPGSPFRVPVKDVVDPSKVKIVGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVMGPRADQTISWSRRSPLKAISGFFKRDRKGDFMETGLVEPVNVVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSTYGVPASLPVEFAIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQTGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMVTEEAYVPVSNMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTAKPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEIDSSDMSAHVTSPSGRVTEAEIVPVGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAHKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYVVPVIAPSDDARRLTVLSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGAGLEGGSTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFLGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP

Target ORF information:

RefSeq Version	XM_005222905.3
Organism	Bos taurus(cattle)
Definition	Bos taurus filamin B (FLNB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222905.3

ORF Insert Sequence:

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG CTGATCAGAC GATTTCCTGG TCAAGGCGCA GCCCCTTGAA AGCCATTTCA 
GGGTTCTTTA AGCGTGACCG GAAGGGTGAC TTTATGGAGA CAGGCCTGGT GGAGCCAGTG 
AACGTGGTGG ACAATGGCGA TGGCACACAC ACAGTGACCT ACACCCCATC CCAGGAGGGC 
CCTTACATGG TCTCTGTTAA GTACGCGGAC GAAGAGATCC CGCGTAGTCC CTTTAAGGTC 
AAGGTCCTTC CCACATACGA TGCCAGCAAA GTGACCGCCA GTGGTCCTGG CCTCAGTACC 
TACGGTGTGC CTGCCAGCCT GCCTGTGGAG TTTGCCATCG ATGCCAGAGA TGCTGGGGAA 
GGCCTGCTTG CTGTTCAGAT AACGGACCAA GAGGGGAAAC CCAAAAGAGC CATTGTCCAC 
GATAATAAAG ATGGCACATA TGCCGTCACT TACATCCCGG ACAAGACTGG ACGGTATATG 
ATTGGGGTCA CCTACGGTGG GGATGACATC CCATTGTCTC CCTACCGCAT CCGGGCCACA 
CAGACGGGTG ATGCCAGCAA GTGCCTGGCC ACGGGGCCTG GAATCGCCTC TACTGTGAAA 
ACCGGCGAGG AAGTGGGCTT TGTGGTCGAT GCCAAGACCG CCGGGAAGGG CAAAGTGACT 
TGCACAGTTT TGACCCCAGA CGGCACCGAG GCTGAGGCCG ATGTCATCGA GAATGAGGAC 
GGCACCTATG ACATCTTCTA CACAGCTGCC AAGCCGGGCA CCTACGTCAT CTATGTGCGC 
TTTGGTGGCG TCGACATTCC CAACAGTCCC TTCACTGTCA TGGTAACAGA AGAGGCCTAT 
GTCCCAGTGA GCAACATGAA CGGCCTGGGA TTTAAGCCTT TCGACTTGGT CATTCCATTT 
GCAGTCAGGA AAGGAGAAAT CACCGGAGAG GTCCACATGC CTTCCGGGAA GACGGCCAAG 
CCCGAGATTG TGGACAACAA GGACGGCACA GTCACTGTCA GATACGCCCC CACGGAGGTT 
GGCCTCCACG AGATGCACAT CAAGTACATG GGCAGCCACA TTCCTGAGAG CCCCCTCCAG 
TTCTACGTGA ACTACCCCAA CAGTGGGAGC GTGTCTGCGT ACGGCCCAGG CCTGGTGTAC 
GGAGTGGCCA ACAAAACCGC CACCTTCACC ATCGTCACCG AAGACGCCGG AGAAGGTGGC 
CTGGACTTGG CTATCGAGGG ACCCTCAAAG GCCGAAATCA GCTGTATTGA CAACAAAGAT 
GGGACGTGCA CGGTGACTTA CCTGCCCACC TTGCCGGGCG ACTACAGCAT CCTGGTCAAG 
TACAATGACA AACACATCCC TGGCAGCCCC TTCACAGCCA AGATCACAGA CGACAGCAGA 
CGGTGCTCTC AGGTGAAGCT GGGCTCAGCC GCCGACTTCT TGCTTGACAT CAGCGAGACT 
GACCTCAGCA CCTTGACGGC CAGCATCAAG GCCCCGTCCG GTCGTGACGA GCCTTGTCTC 
CTGAAGAGGC TGCCCAACAA TCACATCGGC ATCTCCTTCA TTCCCCGGGA GGTGGGTGAA 
CACCTGGTCA GCATCAAGAA GAACGGCAAC CACGTGGCCA ACAGCCCCGT ATCCATCATG 
GTGGTCCAGT CGGAGATTGG GGATGCCCGC AGAGCCAAAG TCTATGGCCG TGGCCTGTCA 
GAAGGCCGGA CTTTCGAGAT GTCTGACTTC ATTGTGGACA CGAGAGATGC AGGTTACGGA 
GGCATCTCCT TGGCGGTGGA AGGCCCTAGC AAGGTGGACA TCCAGACCGA GGACCTGGAG 
GACGGCACCT GCAAGGTCTC CTACTTCCCC ACTGTGCCTG GGGTTTATAT CGTCTCCACC 
AAGTTCGCTG ACGAGCACGT GCCTGGGAGC CCGTTCACCG TGAAAATCAG CGGGGAGGGA 
AGAGTCAAGG AGAGCATCAC CCGTACCAGC CGAGCCCCGT CTGTGGCCAC TGTTGGCAGC 
ATCTGTGACC TGAACCTGAA AATCCCAGAA ATTGACAGCA GTGACATGTC GGCCCACGTC 
ACCAGCCCCT CTGGCCGCGT GACCGAGGCA GAGATCGTGC CGGTGGGGAA GAACTCGCAC 
TGCGTCCGGT TCGTGCCCCA GGAGATGGGC GTTCACACCG TCAGCGTCAA GTACCGTGGC 
CAGCACGTCA CTGGCAGCCC CTTCCAGTTC ACTGTGGGGC CGCTGGGCGA GGGGGGTGCC 
CACAAGGTCC GGGCAGGCGG CCCTGGCCTG GAGCGAGGAG AAGCCGGAGT TCCGGCGGAG 
TTCAGCATCT GGACCCGGGA GGCAGGCGCC GGGGGCCTCT CCATCGCCGT GGAGGGCCCC 
AGTAAGGCCG AGATTACATT CGATGACCAT AAAAACGGAT CTTGTGGTGT GTCTTATATT 
GCCCAGGAAC CTGGTAACTA CGAGGTCTCC ATCAAGTTCA ATGACGAGCA TATTCCCGAA 
AGTCCCTATG TGGTGCCGGT GATCGCGCCC TCGGACGACG CCCGCCGCCT CACTGTTCTG 
AGCCTTCAGG AATCGGGATT AAAAGTTAAT CAGCCAGCCT CCTTTGCTAT AAGGTTGAAC 
GGGGCAAAAG GCAAGATCGA TGCAAAGGTG CACAGCCCCT CTGGAGCCGT GGAGGAATGC 
CACGTGTCTG AGCTGGAGCC AGATAAGTAT GCTGTTCGCT TCATCCCCCA CGAGAATGGT 
ATCCACACGA TTGACGTCAA GTTCAACGGG AGCCACGTGG TTGGAAGTCC CTTCAAAGTG 
CGCGTCGGGG AGCCTGGACA AGCGGGGAAC CCCGCCCTGG TGTCGGCCTA TGGCGCAGGG 
CTTGAGGGAG GCTCCACAGG TATCCAGTCA GAATTTTTCA TCAATACCAC CCGAGCAGGG 
CCAGGGACAT TATCCGTCAC CATCGAAGGG CCGTCCAAGG TTAAAATGGA TTGCCAGGAA 
ACCCCCGAAG GGTACAAGGT CATGTACACC CCCATGGCTC CTGGAAACTA CCTGATTGGC 
GTCAAATATG GCGGGCCCAA CCACATCGTG GGCAGCCCGT TCAAGGCCAA GGTGACAGGC 
CAGCGTCTGG TCAGCCCCGG CTCGGCCAAC GAGACCTCCT CCATCCTGGT GGAGTCAGTG 
ACCAGGTCCT CGACGGAGAC GTGCTACAGC GCCATCCCCA AGGCATCCTC AGACGCCAGC 
AAGGTGACCT CCAAGGGCGC AGGGCTCTCG AAGGCCTTCC TGGGCCAGAA GAGCTCCTTC 
CTGGTGGACT GCAGCAAAGC CGGTTCCAAC ATGTTGCTGA TCGGGGTCCA CGGGCCCACC 
ACCCCCTGCG AAGAAGTCTC CATGAAGCAT GTGGGCAACC AGCAGTACAA CGTGACCTAC 
GTCGTCAAGG AGCGGGGGGA TTACGTCCTG GCCGTGAAGT GGGGAGAGGA GCACATCCCC 
GGCAGTCCGT TCCACGTCAC CGTGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_005222906.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222963.1 Bos taurus filamin B (FLNB), transcript variant X3, mRNA.

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CloneID	OBa31627
Clone ID Related Accession (Same CDS sequence)	XM_005222906.3
Accession Version	XM_005222906.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	filamin-B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222906.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA 
GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA 
GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG 
ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT 
GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG 
GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC 
ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA 
TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG 
GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC 
AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT 
GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG 
CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC 
ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AAATGCATGT 
CCCCCTGGAT TCAGGCCCTG GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC 
GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC 
ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG 
GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC 
AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC 
AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC 
ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA 
CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC 
CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT 
GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG 
GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC 
AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT 
CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG 
AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG 
GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG 
TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA 
GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC 
TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG 
CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC 
CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA 
ATCCCAGAAA TTGACAGCAG TGACATGTCG GCCCACGTCA CCAGCCCCTC TGGCCGCGTG 
ACCGAGGCAG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG 
GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC 
TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC 
CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG 
GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC 
GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC 
GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG 
ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA 
AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT 
GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA 
GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG 
TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA 
GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT 
ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC 
ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC 
ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC 
CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC 
TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG 
TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA 
GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC 
GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC 
ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT 
TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC 
GTGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222963.1
CDS	177..7985
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKHMYRKYHQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPRKPVDNAREAMQQADDWLGVPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEALVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEVASNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDRGNQVYRCVYKPMQPGPHVVKVSFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGIRIRETADFKVDTKAAGSGELGVTIKGPKGLEELVKQKGFQDGVYAFEYYPSTQGKYSVSITWGGHHIQKSPFEVQVGPEAGMQKVRAWGPGLHGGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPFMAYIHPASGDYNPDLVQAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGDGQPIDIQMKSRMDGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVSIGQGSHPQKVKVFGPGVERSGLKAHEPTHFTVDCTEAGEGDVSVGIKCDARVLSDEEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPTSPFRVKVDPSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFSSPGPGDAVKDLDIIDNYDYSHTVKYTPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSRIKINGLENRVEVGKDQEFAVDTRGAGGQGKLDVTILSPSRKVVPCLVAPMAGREGSTAKFIPREEGLYAIDLTYDGHPVPGSPYVVEASLPPDPTKVKAHGPGLRGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDPSKVVASGPGLEHGKVGEPGLLSVDCSEAGPGTLGLEAVSDSGAKAEVCIENNKDGTYAVTYVPLTAGMYTLTMKYGGELVPHFPTRVKVEPAVDTSRVKVFGPGIEGKDVFREATTDFTVDSRPLTQVGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPVPNSPFKVAVTEGCQPSRVQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYVPFAPGDYDVNITYGGAHIPGSPFRVPVKDVVDPSKVKIVGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVMGPRGLVEPVNVVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSTYGVPASLPVEFAIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQTGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMATDGEVAAVKEVPVNACPPGFRPWVTEEAYVPVSNMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTAKPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEIDSSDMSAHVTSPSGRVTEAEIVPVGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAHKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYVVPVIAPSDDARRLTVLSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGAGLEGGSTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFLGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP

Target ORF information:

RefSeq Version	XM_005222906.3
Organism	Bos taurus(cattle)
Definition	Bos taurus filamin B (FLNB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222906.3

ORF Insert Sequence:

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA 
GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA 
GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG 
ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT 
GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG 
GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC 
ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA 
TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG 
GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC 
AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT 
GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG 
CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC 
ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AAATGCATGT 
CCCCCTGGAT TCAGGCCCTG GGTAACAGAA GAGGCCTATG TCCCAGTGAG CAACATGAAC 
GGCCTGGGAT TTAAGCCTTT CGACTTGGTC ATTCCATTTG CAGTCAGGAA AGGAGAAATC 
ACCGGAGAGG TCCACATGCC TTCCGGGAAG ACGGCCAAGC CCGAGATTGT GGACAACAAG 
GACGGCACAG TCACTGTCAG ATACGCCCCC ACGGAGGTTG GCCTCCACGA GATGCACATC 
AAGTACATGG GCAGCCACAT TCCTGAGAGC CCCCTCCAGT TCTACGTGAA CTACCCCAAC 
AGTGGGAGCG TGTCTGCGTA CGGCCCAGGC CTGGTGTACG GAGTGGCCAA CAAAACCGCC 
ACCTTCACCA TCGTCACCGA AGACGCCGGA GAAGGTGGCC TGGACTTGGC TATCGAGGGA 
CCCTCAAAGG CCGAAATCAG CTGTATTGAC AACAAAGATG GGACGTGCAC GGTGACTTAC 
CTGCCCACCT TGCCGGGCGA CTACAGCATC CTGGTCAAGT ACAATGACAA ACACATCCCT 
GGCAGCCCCT TCACAGCCAA GATCACAGAC GACAGCAGAC GGTGCTCTCA GGTGAAGCTG 
GGCTCAGCCG CCGACTTCTT GCTTGACATC AGCGAGACTG ACCTCAGCAC CTTGACGGCC 
AGCATCAAGG CCCCGTCCGG TCGTGACGAG CCTTGTCTCC TGAAGAGGCT GCCCAACAAT 
CACATCGGCA TCTCCTTCAT TCCCCGGGAG GTGGGTGAAC ACCTGGTCAG CATCAAGAAG 
AACGGCAACC ACGTGGCCAA CAGCCCCGTA TCCATCATGG TGGTCCAGTC GGAGATTGGG 
GATGCCCGCA GAGCCAAAGT CTATGGCCGT GGCCTGTCAG AAGGCCGGAC TTTCGAGATG 
TCTGACTTCA TTGTGGACAC GAGAGATGCA GGTTACGGAG GCATCTCCTT GGCGGTGGAA 
GGCCCTAGCA AGGTGGACAT CCAGACCGAG GACCTGGAGG ACGGCACCTG CAAGGTCTCC 
TACTTCCCCA CTGTGCCTGG GGTTTATATC GTCTCCACCA AGTTCGCTGA CGAGCACGTG 
CCTGGGAGCC CGTTCACCGT GAAAATCAGC GGGGAGGGAA GAGTCAAGGA GAGCATCACC 
CGTACCAGCC GAGCCCCGTC TGTGGCCACT GTTGGCAGCA TCTGTGACCT GAACCTGAAA 
ATCCCAGAAA TTGACAGCAG TGACATGTCG GCCCACGTCA CCAGCCCCTC TGGCCGCGTG 
ACCGAGGCAG AGATCGTGCC GGTGGGGAAG AACTCGCACT GCGTCCGGTT CGTGCCCCAG 
GAGATGGGCG TTCACACCGT CAGCGTCAAG TACCGTGGCC AGCACGTCAC TGGCAGCCCC 
TTCCAGTTCA CTGTGGGGCC GCTGGGCGAG GGGGGTGCCC ACAAGGTCCG GGCAGGCGGC 
CCTGGCCTGG AGCGAGGAGA AGCCGGAGTT CCGGCGGAGT TCAGCATCTG GACCCGGGAG 
GCAGGCGCCG GGGGCCTCTC CATCGCCGTG GAGGGCCCCA GTAAGGCCGA GATTACATTC 
GATGACCATA AAAACGGATC TTGTGGTGTG TCTTATATTG CCCAGGAACC TGGTAACTAC 
GAGGTCTCCA TCAAGTTCAA TGACGAGCAT ATTCCCGAAA GTCCCTATGT GGTGCCGGTG 
ATCGCGCCCT CGGACGACGC CCGCCGCCTC ACTGTTCTGA GCCTTCAGGA ATCGGGATTA 
AAAGTTAATC AGCCAGCCTC CTTTGCTATA AGGTTGAACG GGGCAAAAGG CAAGATCGAT 
GCAAAGGTGC ACAGCCCCTC TGGAGCCGTG GAGGAATGCC ACGTGTCTGA GCTGGAGCCA 
GATAAGTATG CTGTTCGCTT CATCCCCCAC GAGAATGGTA TCCACACGAT TGACGTCAAG 
TTCAACGGGA GCCACGTGGT TGGAAGTCCC TTCAAAGTGC GCGTCGGGGA GCCTGGACAA 
GCGGGGAACC CCGCCCTGGT GTCGGCCTAT GGCGCAGGGC TTGAGGGAGG CTCCACAGGT 
ATCCAGTCAG AATTTTTCAT CAATACCACC CGAGCAGGGC CAGGGACATT ATCCGTCACC 
ATCGAAGGGC CGTCCAAGGT TAAAATGGAT TGCCAGGAAA CCCCCGAAGG GTACAAGGTC 
ATGTACACCC CCATGGCTCC TGGAAACTAC CTGATTGGCG TCAAATATGG CGGGCCCAAC 
CACATCGTGG GCAGCCCGTT CAAGGCCAAG GTGACAGGCC AGCGTCTGGT CAGCCCCGGC 
TCGGCCAACG AGACCTCCTC CATCCTGGTG GAGTCAGTGA CCAGGTCCTC GACGGAGACG 
TGCTACAGCG CCATCCCCAA GGCATCCTCA GACGCCAGCA AGGTGACCTC CAAGGGCGCA 
GGGCTCTCGA AGGCCTTCCT GGGCCAGAAG AGCTCCTTCC TGGTGGACTG CAGCAAAGCC 
GGTTCCAACA TGTTGCTGAT CGGGGTCCAC GGGCCCACCA CCCCCTGCGA AGAAGTCTCC 
ATGAAGCATG TGGGCAACCA GCAGTACAAC GTGACCTACG TCGTCAAGGA GCGGGGGGAT 
TACGTCCTGG CCGTGAAGTG GGGAGAGGAG CACATCCCCG GCAGTCCGTT CCACGTCACC 
GTGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_005222907.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222964.1 Bos taurus filamin B (FLNB), transcript variant X4, mRNA.

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CloneID	OBa31628
Clone ID Related Accession (Same CDS sequence)	XM_005222907.3
Accession Version	XM_005222907.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	filamin-B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222907.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA 
GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA 
GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG 
ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT 
GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG 
GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC 
ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA 
TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG 
GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC 
AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT 
GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG 
CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC 
ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AACAGAAGAG 
GCCTATGTCC CAGTGAGCAA CATGAACGGC CTGGGATTTA AGCCTTTCGA CTTGGTCATT 
CCATTTGCAG TCAGGAAAGG AGAAATCACC GGAGAGGTCC ACATGCCTTC CGGGAAGACG 
GCCAAGCCCG AGATTGTGGA CAACAAGGAC GGCACAGTCA CTGTCAGATA CGCCCCCACG 
GAGGTTGGCC TCCACGAGAT GCACATCAAG TACATGGGCA GCCACATTCC TGAGAGCCCC 
CTCCAGTTCT ACGTGAACTA CCCCAACAGT GGGAGCGTGT CTGCGTACGG CCCAGGCCTG 
GTGTACGGAG TGGCCAACAA AACCGCCACC TTCACCATCG TCACCGAAGA CGCCGGAGAA 
GGTGGCCTGG ACTTGGCTAT CGAGGGACCC TCAAAGGCCG AAATCAGCTG TATTGACAAC 
AAAGATGGGA CGTGCACGGT GACTTACCTG CCCACCTTGC CGGGCGACTA CAGCATCCTG 
GTCAAGTACA ATGACAAACA CATCCCTGGC AGCCCCTTCA CAGCCAAGAT CACAGACGAC 
AGCAGACGGT GCTCTCAGGT GAAGCTGGGC TCAGCCGCCG ACTTCTTGCT TGACATCAGC 
GAGACTGACC TCAGCACCTT GACGGCCAGC ATCAAGGCCC CGTCCGGTCG TGACGAGCCT 
TGTCTCCTGA AGAGGCTGCC CAACAATCAC ATCGGCATCT CCTTCATTCC CCGGGAGGTG 
GGTGAACACC TGGTCAGCAT CAAGAAGAAC GGCAACCACG TGGCCAACAG CCCCGTATCC 
ATCATGGTGG TCCAGTCGGA GATTGGGGAT GCCCGCAGAG CCAAAGTCTA TGGCCGTGGC 
CTGTCAGAAG GCCGGACTTT CGAGATGTCT GACTTCATTG TGGACACGAG AGATGCAGGT 
TACGGAGGCA TCTCCTTGGC GGTGGAAGGC CCTAGCAAGG TGGACATCCA GACCGAGGAC 
CTGGAGGACG GCACCTGCAA GGTCTCCTAC TTCCCCACTG TGCCTGGGGT TTATATCGTC 
TCCACCAAGT TCGCTGACGA GCACGTGCCT GGGAGCCCGT TCACCGTGAA AATCAGCGGG 
GAGGGAAGAG TCAAGGAGAG CATCACCCGT ACCAGCCGAG CCCCGTCTGT GGCCACTGTT 
GGCAGCATCT GTGACCTGAA CCTGAAAATC CCAGAAATTG ACAGCAGTGA CATGTCGGCC 
CACGTCACCA GCCCCTCTGG CCGCGTGACC GAGGCAGAGA TCGTGCCGGT GGGGAAGAAC 
TCGCACTGCG TCCGGTTCGT GCCCCAGGAG ATGGGCGTTC ACACCGTCAG CGTCAAGTAC 
CGTGGCCAGC ACGTCACTGG CAGCCCCTTC CAGTTCACTG TGGGGCCGCT GGGCGAGGGG 
GGTGCCCACA AGGTCCGGGC AGGCGGCCCT GGCCTGGAGC GAGGAGAAGC CGGAGTTCCG 
GCGGAGTTCA GCATCTGGAC CCGGGAGGCA GGCGCCGGGG GCCTCTCCAT CGCCGTGGAG 
GGCCCCAGTA AGGCCGAGAT TACATTCGAT GACCATAAAA ACGGATCTTG TGGTGTGTCT 
TATATTGCCC AGGAACCTGG TAACTACGAG GTCTCCATCA AGTTCAATGA CGAGCATATT 
CCCGAAAGTC CCTATGTGGT GCCGGTGATC GCGCCCTCGG ACGACGCCCG CCGCCTCACT 
GTTCTGAGCC TTCAGGAATC GGGATTAAAA GTTAATCAGC CAGCCTCCTT TGCTATAAGG 
TTGAACGGGG CAAAAGGCAA GATCGATGCA AAGGTGCACA GCCCCTCTGG AGCCGTGGAG 
GAATGCCACG TGTCTGAGCT GGAGCCAGAT AAGTATGCTG TTCGCTTCAT CCCCCACGAG 
AATGGTATCC ACACGATTGA CGTCAAGTTC AACGGGAGCC ACGTGGTTGG AAGTCCCTTC 
AAAGTGCGCG TCGGGGAGCC TGGACAAGCG GGGAACCCCG CCCTGGTGTC GGCCTATGGC 
GCAGGGCTTG AGGGAGGCTC CACAGGTATC CAGTCAGAAT TTTTCATCAA TACCACCCGA 
GCAGGGCCAG GGACATTATC CGTCACCATC GAAGGGCCGT CCAAGGTTAA AATGGATTGC 
CAGGAAACCC CCGAAGGGTA CAAGGTCATG TACACCCCCA TGGCTCCTGG AAACTACCTG 
ATTGGCGTCA AATATGGCGG GCCCAACCAC ATCGTGGGCA GCCCGTTCAA GGCCAAGGTG 
ACAGGCCAGC GTCTGGTCAG CCCCGGCTCG GCCAACGAGA CCTCCTCCAT CCTGGTGGAG 
TCAGTGACCA GGTCCTCGAC GGAGACGTGC TACAGCGCCA TCCCCAAGGC ATCCTCAGAC 
GCCAGCAAGG TGACCTCCAA GGGCGCAGGG CTCTCGAAGG CCTTCCTGGG CCAGAAGAGC 
TCCTTCCTGG TGGACTGCAG CAAAGCCGGT TCCAACATGT TGCTGATCGG GGTCCACGGG 
CCCACCACCC CCTGCGAAGA AGTCTCCATG AAGCATGTGG GCAACCAGCA GTACAACGTG 
ACCTACGTCG TCAAGGAGCG GGGGGATTAC GTCCTGGCCG TGAAGTGGGG AGAGGAGCAC 
ATCCCCGGCA GTCCGTTCCA CGTCACCGTG CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222964.1
CDS	177..7952
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKHMYRKYHQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPRKPVDNAREAMQQADDWLGVPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEALVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEVASNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDRGNQVYRCVYKPMQPGPHVVKVSFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGIRIRETADFKVDTKAAGSGELGVTIKGPKGLEELVKQKGFQDGVYAFEYYPSTQGKYSVSITWGGHHIQKSPFEVQVGPEAGMQKVRAWGPGLHGGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPFMAYIHPASGDYNPDLVQAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGDGQPIDIQMKSRMDGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVSIGQGSHPQKVKVFGPGVERSGLKAHEPTHFTVDCTEAGEGDVSVGIKCDARVLSDEEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPTSPFRVKVDPSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFSSPGPGDAVKDLDIIDNYDYSHTVKYTPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSRIKINGLENRVEVGKDQEFAVDTRGAGGQGKLDVTILSPSRKVVPCLVAPMAGREGSTAKFIPREEGLYAIDLTYDGHPVPGSPYVVEASLPPDPTKVKAHGPGLRGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDPSKVVASGPGLEHGKVGEPGLLSVDCSEAGPGTLGLEAVSDSGAKAEVCIENNKDGTYAVTYVPLTAGMYTLTMKYGGELVPHFPTRVKVEPAVDTSRVKVFGPGIEGKDVFREATTDFTVDSRPLTQVGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPVPNSPFKVAVTEGCQPSRVQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYVPFAPGDYDVNITYGGAHIPGSPFRVPVKDVVDPSKVKIVGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVMGPRGLVEPVNVVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSTYGVPASLPVEFAIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQTGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMATDGEVAAVKEVPVTEEAYVPVSNMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTAKPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEIDSSDMSAHVTSPSGRVTEAEIVPVGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAHKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYVVPVIAPSDDARRLTVLSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGAGLEGGSTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFLGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP

Target ORF information:

RefSeq Version	XM_005222907.3
Organism	Bos taurus(cattle)
Definition	Bos taurus filamin B (FLNB), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222907.3

ORF Insert Sequence:

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA 
GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA 
GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG 
ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT 
GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG 
GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC 
ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA 
TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG 
GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC 
AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT 
GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG 
CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC 
ACTGTCATGG CCACAGATGG GGAAGTCGCA GCTGTGAAGG AAGTACCGGT AACAGAAGAG 
GCCTATGTCC CAGTGAGCAA CATGAACGGC CTGGGATTTA AGCCTTTCGA CTTGGTCATT 
CCATTTGCAG TCAGGAAAGG AGAAATCACC GGAGAGGTCC ACATGCCTTC CGGGAAGACG 
GCCAAGCCCG AGATTGTGGA CAACAAGGAC GGCACAGTCA CTGTCAGATA CGCCCCCACG 
GAGGTTGGCC TCCACGAGAT GCACATCAAG TACATGGGCA GCCACATTCC TGAGAGCCCC 
CTCCAGTTCT ACGTGAACTA CCCCAACAGT GGGAGCGTGT CTGCGTACGG CCCAGGCCTG 
GTGTACGGAG TGGCCAACAA AACCGCCACC TTCACCATCG TCACCGAAGA CGCCGGAGAA 
GGTGGCCTGG ACTTGGCTAT CGAGGGACCC TCAAAGGCCG AAATCAGCTG TATTGACAAC 
AAAGATGGGA CGTGCACGGT GACTTACCTG CCCACCTTGC CGGGCGACTA CAGCATCCTG 
GTCAAGTACA ATGACAAACA CATCCCTGGC AGCCCCTTCA CAGCCAAGAT CACAGACGAC 
AGCAGACGGT GCTCTCAGGT GAAGCTGGGC TCAGCCGCCG ACTTCTTGCT TGACATCAGC 
GAGACTGACC TCAGCACCTT GACGGCCAGC ATCAAGGCCC CGTCCGGTCG TGACGAGCCT 
TGTCTCCTGA AGAGGCTGCC CAACAATCAC ATCGGCATCT CCTTCATTCC CCGGGAGGTG 
GGTGAACACC TGGTCAGCAT CAAGAAGAAC GGCAACCACG TGGCCAACAG CCCCGTATCC 
ATCATGGTGG TCCAGTCGGA GATTGGGGAT GCCCGCAGAG CCAAAGTCTA TGGCCGTGGC 
CTGTCAGAAG GCCGGACTTT CGAGATGTCT GACTTCATTG TGGACACGAG AGATGCAGGT 
TACGGAGGCA TCTCCTTGGC GGTGGAAGGC CCTAGCAAGG TGGACATCCA GACCGAGGAC 
CTGGAGGACG GCACCTGCAA GGTCTCCTAC TTCCCCACTG TGCCTGGGGT TTATATCGTC 
TCCACCAAGT TCGCTGACGA GCACGTGCCT GGGAGCCCGT TCACCGTGAA AATCAGCGGG 
GAGGGAAGAG TCAAGGAGAG CATCACCCGT ACCAGCCGAG CCCCGTCTGT GGCCACTGTT 
GGCAGCATCT GTGACCTGAA CCTGAAAATC CCAGAAATTG ACAGCAGTGA CATGTCGGCC 
CACGTCACCA GCCCCTCTGG CCGCGTGACC GAGGCAGAGA TCGTGCCGGT GGGGAAGAAC 
TCGCACTGCG TCCGGTTCGT GCCCCAGGAG ATGGGCGTTC ACACCGTCAG CGTCAAGTAC 
CGTGGCCAGC ACGTCACTGG CAGCCCCTTC CAGTTCACTG TGGGGCCGCT GGGCGAGGGG 
GGTGCCCACA AGGTCCGGGC AGGCGGCCCT GGCCTGGAGC GAGGAGAAGC CGGAGTTCCG 
GCGGAGTTCA GCATCTGGAC CCGGGAGGCA GGCGCCGGGG GCCTCTCCAT CGCCGTGGAG 
GGCCCCAGTA AGGCCGAGAT TACATTCGAT GACCATAAAA ACGGATCTTG TGGTGTGTCT 
TATATTGCCC AGGAACCTGG TAACTACGAG GTCTCCATCA AGTTCAATGA CGAGCATATT 
CCCGAAAGTC CCTATGTGGT GCCGGTGATC GCGCCCTCGG ACGACGCCCG CCGCCTCACT 
GTTCTGAGCC TTCAGGAATC GGGATTAAAA GTTAATCAGC CAGCCTCCTT TGCTATAAGG 
TTGAACGGGG CAAAAGGCAA GATCGATGCA AAGGTGCACA GCCCCTCTGG AGCCGTGGAG 
GAATGCCACG TGTCTGAGCT GGAGCCAGAT AAGTATGCTG TTCGCTTCAT CCCCCACGAG 
AATGGTATCC ACACGATTGA CGTCAAGTTC AACGGGAGCC ACGTGGTTGG AAGTCCCTTC 
AAAGTGCGCG TCGGGGAGCC TGGACAAGCG GGGAACCCCG CCCTGGTGTC GGCCTATGGC 
GCAGGGCTTG AGGGAGGCTC CACAGGTATC CAGTCAGAAT TTTTCATCAA TACCACCCGA 
GCAGGGCCAG GGACATTATC CGTCACCATC GAAGGGCCGT CCAAGGTTAA AATGGATTGC 
CAGGAAACCC CCGAAGGGTA CAAGGTCATG TACACCCCCA TGGCTCCTGG AAACTACCTG 
ATTGGCGTCA AATATGGCGG GCCCAACCAC ATCGTGGGCA GCCCGTTCAA GGCCAAGGTG 
ACAGGCCAGC GTCTGGTCAG CCCCGGCTCG GCCAACGAGA CCTCCTCCAT CCTGGTGGAG 
TCAGTGACCA GGTCCTCGAC GGAGACGTGC TACAGCGCCA TCCCCAAGGC ATCCTCAGAC 
GCCAGCAAGG TGACCTCCAA GGGCGCAGGG CTCTCGAAGG CCTTCCTGGG CCAGAAGAGC 
TCCTTCCTGG TGGACTGCAG CAAAGCCGGT TCCAACATGT TGCTGATCGG GGTCCACGGG 
CCCACCACCC CCTGCGAAGA AGTCTCCATG AAGCATGTGG GCAACCAGCA GTACAACGTG 
ACCTACGTCG TCAAGGAGCG GGGGGATTAC GTCCTGGCCG TGAAGTGGGG AGAGGAGCAC 
ATCCCCGGCA GTCCGTTCCA CGTCACCGTG CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FLNB ( XM_005222908.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222965.1 Bos taurus filamin B (FLNB), transcript variant X5, mRNA.

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CloneID	OBa31629
Clone ID Related Accession (Same CDS sequence)	XM_005222908.3
Accession Version	XM_005222908.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	filamin-B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222908.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA 
GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA 
GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG 
ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT 
GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG 
GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC 
ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA 
TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG 
GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC 
AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT 
GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG 
CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC 
ACTGTCATGG TAACAGAAGA GGCCTATGTC CCAGTGAGCA ACATGAACGG CCTGGGATTT 
AAGCCTTTCG ACTTGGTCAT TCCATTTGCA GTCAGGAAAG GAGAAATCAC CGGAGAGGTC 
CACATGCCTT CCGGGAAGAC GGCCAAGCCC GAGATTGTGG ACAACAAGGA CGGCACAGTC 
ACTGTCAGAT ACGCCCCCAC GGAGGTTGGC CTCCACGAGA TGCACATCAA GTACATGGGC 
AGCCACATTC CTGAGAGCCC CCTCCAGTTC TACGTGAACT ACCCCAACAG TGGGAGCGTG 
TCTGCGTACG GCCCAGGCCT GGTGTACGGA GTGGCCAACA AAACCGCCAC CTTCACCATC 
GTCACCGAAG ACGCCGGAGA AGGTGGCCTG GACTTGGCTA TCGAGGGACC CTCAAAGGCC 
GAAATCAGCT GTATTGACAA CAAAGATGGG ACGTGCACGG TGACTTACCT GCCCACCTTG 
CCGGGCGACT ACAGCATCCT GGTCAAGTAC AATGACAAAC ACATCCCTGG CAGCCCCTTC 
ACAGCCAAGA TCACAGACGA CAGCAGACGG TGCTCTCAGG TGAAGCTGGG CTCAGCCGCC 
GACTTCTTGC TTGACATCAG CGAGACTGAC CTCAGCACCT TGACGGCCAG CATCAAGGCC 
CCGTCCGGTC GTGACGAGCC TTGTCTCCTG AAGAGGCTGC CCAACAATCA CATCGGCATC 
TCCTTCATTC CCCGGGAGGT GGGTGAACAC CTGGTCAGCA TCAAGAAGAA CGGCAACCAC 
GTGGCCAACA GCCCCGTATC CATCATGGTG GTCCAGTCGG AGATTGGGGA TGCCCGCAGA 
GCCAAAGTCT ATGGCCGTGG CCTGTCAGAA GGCCGGACTT TCGAGATGTC TGACTTCATT 
GTGGACACGA GAGATGCAGG TTACGGAGGC ATCTCCTTGG CGGTGGAAGG CCCTAGCAAG 
GTGGACATCC AGACCGAGGA CCTGGAGGAC GGCACCTGCA AGGTCTCCTA CTTCCCCACT 
GTGCCTGGGG TTTATATCGT CTCCACCAAG TTCGCTGACG AGCACGTGCC TGGGAGCCCG 
TTCACCGTGA AAATCAGCGG GGAGGGAAGA GTCAAGGAGA GCATCACCCG TACCAGCCGA 
GCCCCGTCTG TGGCCACTGT TGGCAGCATC TGTGACCTGA ACCTGAAAAT CCCAGAAATT 
GACAGCAGTG ACATGTCGGC CCACGTCACC AGCCCCTCTG GCCGCGTGAC CGAGGCAGAG 
ATCGTGCCGG TGGGGAAGAA CTCGCACTGC GTCCGGTTCG TGCCCCAGGA GATGGGCGTT 
CACACCGTCA GCGTCAAGTA CCGTGGCCAG CACGTCACTG GCAGCCCCTT CCAGTTCACT 
GTGGGGCCGC TGGGCGAGGG GGGTGCCCAC AAGGTCCGGG CAGGCGGCCC TGGCCTGGAG 
CGAGGAGAAG CCGGAGTTCC GGCGGAGTTC AGCATCTGGA CCCGGGAGGC AGGCGCCGGG 
GGCCTCTCCA TCGCCGTGGA GGGCCCCAGT AAGGCCGAGA TTACATTCGA TGACCATAAA 
AACGGATCTT GTGGTGTGTC TTATATTGCC CAGGAACCTG GTAACTACGA GGTCTCCATC 
AAGTTCAATG ACGAGCATAT TCCCGAAAGT CCCTATGTGG TGCCGGTGAT CGCGCCCTCG 
GACGACGCCC GCCGCCTCAC TGTTCTGAGC CTTCAGGAAT CGGGATTAAA AGTTAATCAG 
CCAGCCTCCT TTGCTATAAG GTTGAACGGG GCAAAAGGCA AGATCGATGC AAAGGTGCAC 
AGCCCCTCTG GAGCCGTGGA GGAATGCCAC GTGTCTGAGC TGGAGCCAGA TAAGTATGCT 
GTTCGCTTCA TCCCCCACGA GAATGGTATC CACACGATTG ACGTCAAGTT CAACGGGAGC 
CACGTGGTTG GAAGTCCCTT CAAAGTGCGC GTCGGGGAGC CTGGACAAGC GGGGAACCCC 
GCCCTGGTGT CGGCCTATGG CGCAGGGCTT GAGGGAGGCT CCACAGGTAT CCAGTCAGAA 
TTTTTCATCA ATACCACCCG AGCAGGGCCA GGGACATTAT CCGTCACCAT CGAAGGGCCG 
TCCAAGGTTA AAATGGATTG CCAGGAAACC CCCGAAGGGT ACAAGGTCAT GTACACCCCC 
ATGGCTCCTG GAAACTACCT GATTGGCGTC AAATATGGCG GGCCCAACCA CATCGTGGGC 
AGCCCGTTCA AGGCCAAGGT GACAGGCCAG CGTCTGGTCA GCCCCGGCTC GGCCAACGAG 
ACCTCCTCCA TCCTGGTGGA GTCAGTGACC AGGTCCTCGA CGGAGACGTG CTACAGCGCC 
ATCCCCAAGG CATCCTCAGA CGCCAGCAAG GTGACCTCCA AGGGCGCAGG GCTCTCGAAG 
GCCTTCCTGG GCCAGAAGAG CTCCTTCCTG GTGGACTGCA GCAAAGCCGG TTCCAACATG 
TTGCTGATCG GGGTCCACGG GCCCACCACC CCCTGCGAAG AAGTCTCCAT GAAGCATGTG 
GGCAACCAGC AGTACAACGT GACCTACGTC GTCAAGGAGC GGGGGGATTA CGTCCTGGCC 
GTGAAGTGGG GAGAGGAGCA CATCCCCGGC AGTCCGTTCC ACGTCACCGT GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222965.1
CDS	177..7913
Translation	MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKHMYRKYHQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPRKPVDNAREAMQQADDWLGVPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEALVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVNVDKAQGDASKVTAKGPGLEVASNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDRGNQVYRCVYKPMQPGPHVVKVSFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGIRIRETADFKVDTKAAGSGELGVTIKGPKGLEELVKQKGFQDGVYAFEYYPSTQGKYSVSITWGGHHIQKSPFEVQVGPEAGMQKVRAWGPGLHGGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPFMAYIHPASGDYNPDLVQAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGDGQPIDIQMKSRMDGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVSIGQGSHPQKVKVFGPGVERSGLKAHEPTHFTVDCTEAGEGDVSVGIKCDARVLSDEEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPTSPFRVKVDPSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFSSPGPGDAVKDLDIIDNYDYSHTVKYTPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSRIKINGLENRVEVGKDQEFAVDTRGAGGQGKLDVTILSPSRKVVPCLVAPMAGREGSTAKFIPREEGLYAIDLTYDGHPVPGSPYVVEASLPPDPTKVKAHGPGLRGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDPSKVVASGPGLEHGKVGEPGLLSVDCSEAGPGTLGLEAVSDSGAKAEVCIENNKDGTYAVTYVPLTAGMYTLTMKYGGELVPHFPTRVKVEPAVDTSRVKVFGPGIEGKDVFREATTDFTVDSRPLTQVGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPVPNSPFKVAVTEGCQPSRVQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYVPFAPGDYDVNITYGGAHIPGSPFRVPVKDVVDPSKVKIVGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVMGPRGLVEPVNVVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSTYGVPASLPVEFAIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQTGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMVTEEAYVPVSNMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTAKPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEIDSSDMSAHVTSPSGRVTEAEIVPVGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAHKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYVVPVIAPSDDARRLTVLSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGAGLEGGSTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLIGVKYGGPNHIVGSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFLGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP

Target ORF information:

RefSeq Version	XM_005222908.3
Organism	Bos taurus(cattle)
Definition	Bos taurus filamin B (FLNB), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222908.3

ORF Insert Sequence:

ATGCCCGTAA CCGAGAAGGA CCTGGCGGAG GACGCGCCCT GGAAGAAGAT CCAGCAGAAC 
ACCTTCACGC GTTGGTGCAA CGAGCACCTT AAGTGCGTGA ACAAGCGCAT AGGGAACCTG 
CAGACCGACC TGAGCGACGG GCTGCGGCTC ATCGCGCTCC TGGAGGTGCT CAGCCAGAAG 
CACATGTACC GCAAGTACCA CCAGCGGCCC ACCTTCCGCC AGATGCAGCT GGAGAACGTG 
TCCGTGGCGC TCGAGTTCCT GGACCGCGAG AGCATCAAGC TCGTGTCCAT CGACAGCAAA 
GCCATCGTGG ATGGGAACCT GAAGCTCATC TTGGGCCTGG TGTGGACGCT CATCCTCCAC 
TACTCCATCT CCATGCCCGT GTGGGAGGAT GAAGGAGACG ATGATGCCAA GAAGCAGACG 
CCAAAGCAGA GGCTGCTTGG GTGGATTCAG AACAAGATCC CCTACTTGCC CATCACCAAC 
TTCAACCAAA ACTGGCAAGA CGGCAAAGCC CTGGGAGCCT TGGTAGACAG TTGTGCTCCA 
GGTCTTTGCC CAGACTGGGA ATCCTGGGAT CCACGAAAGC CTGTGGATAA TGCACGAGAA 
GCCATGCAGC AGGCAGACGA CTGGCTAGGC GTCCCACAGG TCATCACTCC TGAAGAAATT 
ATTCACCCGG ATGTGGATGA ACACTCAGTG ATGACTTACC TGTCCCAGTT CCCCAAAGCC 
AAGCTCAAGC CTGGAGCTCC CCTTAAACCC AAACTCAACC CCAAGAAAGC CAGGGCCTAT 
GGTAGAGGTA TCGAGCCCAC TGGGAACATG GTGAAGCAGC CGGCCAAGTT CACCGTGGAC 
ACAATCAGCG CCGGGCAAGG TGATGTCATG GTGTTTGTTG AGGACCCAGA AGGGAACAAA 
GAGGAGGCAC TCGTGACCCC TGACAGCGAT AAGAACAAGA CATACTCTGT GGAGTATCTG 
CCCAAGGTCA CTGGACTGCA CAAAGTCACA GTCCTCTTTG CAGGACAGCA CATCTCCAAG 
AGCCCATTCG AGGTGAATGT CGACAAGGCC CAGGGAGATG CCAGTAAAGT CACTGCGAAA 
GGCCCAGGCT TGGAAGTTGC CAGCAACATC GCCAATAAGC CCACCTATTT TGACATCTAC 
ACAGCAGGTG CTGGCGTGGG CGACATTGGT GTGGAGGTGG AGGATCCCCA GGGGAAGAAC 
ACCGTGGAGT TGCTCGTGGA AGACAGAGGA AACCAAGTGT ATCGATGTGT GTACAAACCA 
ATGCAGCCTG GCCCCCACGT GGTCAAGGTT TCCTTTGCTG GGGACACCAT CCCCAAGAGT 
CCCTTCGTTG TTCAGGTTGG GGAAGCCTGC AATCCAAACG CCTGCCGGGC CAGTGGCCGA 
GGCCTGCAGC CTAAAGGCAT TCGCATCCGA GAAACTGCAG ACTTCAAGGT TGACACCAAA 
GCTGCTGGAA GTGGAGAGCT CGGCGTCACC ATTAAGGGTC CTAAGGGTCT GGAGGAGCTG 
GTGAAACAGA AAGGCTTTCA GGATGGAGTC TATGCATTTG AGTATTACCC CAGCACCCAG 
GGGAAATACA GCGTCTCCAT CACGTGGGGA GGACACCACA TTCAAAAGAG CCCTTTTGAA 
GTTCAAGTTG GCCCTGAAGC AGGCATGCAG AAAGTCCGTG CATGGGGACC TGGCCTCCAC 
GGCGGGATTG TTGGGCGGTC AGCGGACTTC GTTGTGGAGT CCATTGGTTC TGAAGTGGGG 
TCTCTGGGGT TTGCCATTGA AGGCCCCTCC CAGGCGAAGA TTGAATATGA CGACCAGAAT 
GACGGGTCAT GTGATGTCAA GTACTGGCCC AAGGAGCCGG GCGAATATGC CGTTCACATC 
ATGTGCGACG ATGAGGACAT CAAGGACAGT CCCTTCATGG CCTACATCCA CCCGGCCTCG 
GGAGACTACA ACCCGGACCT GGTGCAAGCA TACGGGCCAG GTTTGGAGAA ATCCGGATGC 
ATTGTCAACA ACCTGGCTGA GTTCACCGTG GATCCTAAGG ATGCCGGAAA AGCTCCCTTA 
AAGATATTTG CTCAGGACGG GGACGGCCAG CCTATTGACA TCCAGATGAA GAGCCGGATG 
GATGGCACCT ACGCCTGCTC ATACACCCCA GTTAAGGCCA TCAAGCACAC CATTGCCGTG 
GTCTGGGGAG GTGTCAACAT CCCACACAGC CCTTACCGGG TCAGCATCGG ACAGGGTAGC 
CATCCCCAGA AGGTCAAAGT GTTTGGGCCA GGAGTGGAGA GAAGTGGACT GAAGGCACAC 
GAGCCTACTC ACTTCACCGT GGACTGTACT GAGGCTGGCG AAGGTGATGT CAGCGTTGGC 
ATTAAATGTG ATGCCCGGGT GTTAAGTGAC GAGGAGGAAG ACGTGGATTT TGACATTATT 
CACAACGCCA ATGATACATT TACTGTCAAA TATGTGCCTC CTGCTGCCGG GCGATACACC 
ATCAAAGTTC TCTTTGCCTC TCAGGAAATC CCCACCAGCC CTTTCAGAGT CAAGGTCGAC 
CCTTCCCACG ATGCCAGCAA AGTGAAGGCA GAAGGCCCAG GGCTCAGCAA AGCAGGTGTG 
GAAAACGGGA AACCCACCCA CTTCACTGTG TACACCAAGG GGGCTGGAAA GGCCCCGCTC 
AACGTGCAGT TCAGCAGCCC TGGCCCTGGC GACGCCGTGA AGGATCTGGA TATCATCGAT 
AACTATGACT ACTCTCACAC CGTTAAATAC ACACCCACCC AGCAGGGCAA CATGCAGGTT 
CTGGTTACAT ACGGTGGTGA CCCTATCCCT AAGAGCCCAT TCACTGTGGG TGTGGCTGCT 
CCGCTGGATC TGAGCAGGAT AAAAATTAAT GGACTGGAAA ACAGAGTCGA AGTTGGGAAG 
GACCAGGAGT TCGCCGTGGA CACCAGGGGA GCAGGAGGCC AGGGGAAGCT GGACGTGACG 
ATCCTGAGCC CCTCAAGGAA GGTCGTGCCG TGTCTGGTGG CACCCATGGC AGGCCGGGAG 
GGCAGCACAG CCAAGTTCAT CCCACGGGAG GAAGGGCTGT ATGCCATCGA CTTGACCTAT 
GACGGACATC CTGTGCCCGG AAGCCCTTAC GTGGTGGAGG CCTCGCTGCC ACCTGATCCC 
ACCAAGGTGA AGGCCCATGG TCCTGGCCTC AGAGGTGGTC TTGTGGGCAA GCCTGCCGAG 
TTCACCATCG ACACCAAAGG AGCTGGAACT GGAGGTCTCG GCCTAACCGT GGAAGGCCCA 
TGCGAGGCCA AAATTGAATG CTCAGACAAC GGCGATGGGA CCTGTTCTGT CTCCTACCTC 
CCGACAAAGC CCGGGGAGTA CTTCGTCAAC ATTCTCTTCG AAGAAGTCCA CATACCTGGC 
TCTCCCTTCA AAGCCGACAT TGAAATGCCT TTTGACCCCT CCAAAGTCGT GGCGTCCGGC 
CCGGGCCTCG AGCATGGGAA GGTGGGCGAG CCCGGGCTGC TCAGCGTGGA CTGTTCAGAA 
GCTGGGCCAG GGACCCTGGG CCTGGAAGCT GTCTCGGACT CAGGGGCAAA AGCCGAAGTC 
TGTATCGAGA ACAACAAAGA TGGTACCTAT GCGGTGACCT ATGTGCCCCT GACGGCCGGC 
ATGTACACGC TGACCATGAA GTACGGCGGG GAGCTGGTGC CACACTTCCC CACCAGGGTC 
AAGGTGGAGC CCGCCGTGGA CACCAGTAGG GTCAAAGTCT TTGGGCCGGG AATAGAAGGA 
AAAGACGTGT TTCGTGAAGC CACCACCGAT TTCACGGTGG ACTCACGGCC CCTGACGCAG 
GTCGGAGGCG ACCACATCAA GGCCCACATT GCCAACCCCT CCGGAGCCTC CACGGAGTGT 
TTCGTCACAG ACAATGCTGA TGGGACCTAC CAGGTGGAAT ACACGCCCTT CGAGAAAGGC 
CTTCACGTAG TGGAGGTAAC CTACGATGAC GTGCCTGTCC CAAATAGTCC CTTTAAAGTG 
GCCGTGACCG AAGGCTGCCA GCCTTCTCGG GTTCAAGCTC AAGGACCTGG ATTGAAGGAG 
GCTTTCACCA ACAAGCCCAA CGTTTTCACG GTGGTTACCA GAGGGGCAGG AATTGGGGGT 
CTCGGCATAA CTGTGGAGGG ACCGTCCGAG TCAAAGATAA ACTGCAGAGA CAACAAAGAT 
GGCAGCTGCA GTGCAGAGTA CGTTCCCTTT GCTCCGGGAG ATTACGACGT GAATATCACA 
TACGGAGGTG CCCACATTCC TGGCAGCCCC TTCAGGGTTC CTGTGAAGGA TGTTGTGGAC 
CCCAGCAAGG TCAAGATCGT TGGCCCTGGG CTGGGCTCCG GTGTCCGAGC TCGCGTCTTG 
CAGTCCTTCA CGGTGGACAG CAGCAAGGCT GGCCTGGCCC CTCTGGAAGT GAGGGTCATG 
GGTCCACGAG GCCTGGTGGA GCCAGTGAAC GTGGTGGACA ATGGCGATGG CACACACACA 
GTGACCTACA CCCCATCCCA GGAGGGCCCT TACATGGTCT CTGTTAAGTA CGCGGACGAA 
GAGATCCCGC GTAGTCCCTT TAAGGTCAAG GTCCTTCCCA CATACGATGC CAGCAAAGTG 
ACCGCCAGTG GTCCTGGCCT CAGTACCTAC GGTGTGCCTG CCAGCCTGCC TGTGGAGTTT 
GCCATCGATG CCAGAGATGC TGGGGAAGGC CTGCTTGCTG TTCAGATAAC GGACCAAGAG 
GGGAAACCCA AAAGAGCCAT TGTCCACGAT AATAAAGATG GCACATATGC CGTCACTTAC 
ATCCCGGACA AGACTGGACG GTATATGATT GGGGTCACCT ACGGTGGGGA TGACATCCCA 
TTGTCTCCCT ACCGCATCCG GGCCACACAG ACGGGTGATG CCAGCAAGTG CCTGGCCACG 
GGGCCTGGAA TCGCCTCTAC TGTGAAAACC GGCGAGGAAG TGGGCTTTGT GGTCGATGCC 
AAGACCGCCG GGAAGGGCAA AGTGACTTGC ACAGTTTTGA CCCCAGACGG CACCGAGGCT 
GAGGCCGATG TCATCGAGAA TGAGGACGGC ACCTATGACA TCTTCTACAC AGCTGCCAAG 
CCGGGCACCT ACGTCATCTA TGTGCGCTTT GGTGGCGTCG ACATTCCCAA CAGTCCCTTC 
ACTGTCATGG TAACAGAAGA GGCCTATGTC CCAGTGAGCA ACATGAACGG CCTGGGATTT 
AAGCCTTTCG ACTTGGTCAT TCCATTTGCA GTCAGGAAAG GAGAAATCAC CGGAGAGGTC 
CACATGCCTT CCGGGAAGAC GGCCAAGCCC GAGATTGTGG ACAACAAGGA CGGCACAGTC 
ACTGTCAGAT ACGCCCCCAC GGAGGTTGGC CTCCACGAGA TGCACATCAA GTACATGGGC 
AGCCACATTC CTGAGAGCCC CCTCCAGTTC TACGTGAACT ACCCCAACAG TGGGAGCGTG 
TCTGCGTACG GCCCAGGCCT GGTGTACGGA GTGGCCAACA AAACCGCCAC CTTCACCATC 
GTCACCGAAG ACGCCGGAGA AGGTGGCCTG GACTTGGCTA TCGAGGGACC CTCAAAGGCC 
GAAATCAGCT GTATTGACAA CAAAGATGGG ACGTGCACGG TGACTTACCT GCCCACCTTG 
CCGGGCGACT ACAGCATCCT GGTCAAGTAC AATGACAAAC ACATCCCTGG CAGCCCCTTC 
ACAGCCAAGA TCACAGACGA CAGCAGACGG TGCTCTCAGG TGAAGCTGGG CTCAGCCGCC 
GACTTCTTGC TTGACATCAG CGAGACTGAC CTCAGCACCT TGACGGCCAG CATCAAGGCC 
CCGTCCGGTC GTGACGAGCC TTGTCTCCTG AAGAGGCTGC CCAACAATCA CATCGGCATC 
TCCTTCATTC CCCGGGAGGT GGGTGAACAC CTGGTCAGCA TCAAGAAGAA CGGCAACCAC 
GTGGCCAACA GCCCCGTATC CATCATGGTG GTCCAGTCGG AGATTGGGGA TGCCCGCAGA 
GCCAAAGTCT ATGGCCGTGG CCTGTCAGAA GGCCGGACTT TCGAGATGTC TGACTTCATT 
GTGGACACGA GAGATGCAGG TTACGGAGGC ATCTCCTTGG CGGTGGAAGG CCCTAGCAAG 
GTGGACATCC AGACCGAGGA CCTGGAGGAC GGCACCTGCA AGGTCTCCTA CTTCCCCACT 
GTGCCTGGGG TTTATATCGT CTCCACCAAG TTCGCTGACG AGCACGTGCC TGGGAGCCCG 
TTCACCGTGA AAATCAGCGG GGAGGGAAGA GTCAAGGAGA GCATCACCCG TACCAGCCGA 
GCCCCGTCTG TGGCCACTGT TGGCAGCATC TGTGACCTGA ACCTGAAAAT CCCAGAAATT 
GACAGCAGTG ACATGTCGGC CCACGTCACC AGCCCCTCTG GCCGCGTGAC CGAGGCAGAG 
ATCGTGCCGG TGGGGAAGAA CTCGCACTGC GTCCGGTTCG TGCCCCAGGA GATGGGCGTT 
CACACCGTCA GCGTCAAGTA CCGTGGCCAG CACGTCACTG GCAGCCCCTT CCAGTTCACT 
GTGGGGCCGC TGGGCGAGGG GGGTGCCCAC AAGGTCCGGG CAGGCGGCCC TGGCCTGGAG 
CGAGGAGAAG CCGGAGTTCC GGCGGAGTTC AGCATCTGGA CCCGGGAGGC AGGCGCCGGG 
GGCCTCTCCA TCGCCGTGGA GGGCCCCAGT AAGGCCGAGA TTACATTCGA TGACCATAAA 
AACGGATCTT GTGGTGTGTC TTATATTGCC CAGGAACCTG GTAACTACGA GGTCTCCATC 
AAGTTCAATG ACGAGCATAT TCCCGAAAGT CCCTATGTGG TGCCGGTGAT CGCGCCCTCG 
GACGACGCCC GCCGCCTCAC TGTTCTGAGC CTTCAGGAAT CGGGATTAAA AGTTAATCAG 
CCAGCCTCCT TTGCTATAAG GTTGAACGGG GCAAAAGGCA AGATCGATGC AAAGGTGCAC 
AGCCCCTCTG GAGCCGTGGA GGAATGCCAC GTGTCTGAGC TGGAGCCAGA TAAGTATGCT 
GTTCGCTTCA TCCCCCACGA GAATGGTATC CACACGATTG ACGTCAAGTT CAACGGGAGC 
CACGTGGTTG GAAGTCCCTT CAAAGTGCGC GTCGGGGAGC CTGGACAAGC GGGGAACCCC 
GCCCTGGTGT CGGCCTATGG CGCAGGGCTT GAGGGAGGCT CCACAGGTAT CCAGTCAGAA 
TTTTTCATCA ATACCACCCG AGCAGGGCCA GGGACATTAT CCGTCACCAT CGAAGGGCCG 
TCCAAGGTTA AAATGGATTG CCAGGAAACC CCCGAAGGGT ACAAGGTCAT GTACACCCCC 
ATGGCTCCTG GAAACTACCT GATTGGCGTC AAATATGGCG GGCCCAACCA CATCGTGGGC 
AGCCCGTTCA AGGCCAAGGT GACAGGCCAG CGTCTGGTCA GCCCCGGCTC GGCCAACGAG 
ACCTCCTCCA TCCTGGTGGA GTCAGTGACC AGGTCCTCGA CGGAGACGTG CTACAGCGCC 
ATCCCCAAGG CATCCTCAGA CGCCAGCAAG GTGACCTCCA AGGGCGCAGG GCTCTCGAAG 
GCCTTCCTGG GCCAGAAGAG CTCCTTCCTG GTGGACTGCA GCAAAGCCGG TTCCAACATG 
TTGCTGATCG GGGTCCACGG GCCCACCACC CCCTGCGAAG AAGTCTCCAT GAAGCATGTG 
GGCAACCAGC AGTACAACGT GACCTACGTC GTCAAGGAGC GGGGGGATTA CGTCCTGGCC 
GTGAAGTGGG GAGAGGAGCA CATCCCCGGC AGTCCGTTCC ACGTCACCGT GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	FLNB
Entrez Gene ID	613533
Full Name	filamin B
General protein information	Preferred Names filamin B Names filamin-B filamin B, beta (actin binding protein 278)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Canis lupus familiaris (dog)	FLNB	XP_003432942.1
Xenopus tropicalis (tropical clawed frog)	flnb	XP_004914227.1
Pan troglodytes (chimpanzee)	FLNB	XP_001174012.2
Bos taurus (cattle)	FLNB	NP_001178389.1
Rattus norvegicus (Norway rat)	Flnb	NP_001100758.1
Homo sapiens (human)	FLNB	NP_001157789.1
	FLNB	XP_001097922.2
Mus musculus (house mouse)	Flnb	NP_598841.1
Gallus gallus (chicken)	FLNB	NP_001025512.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

FLNB cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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