TRPM8 cDNA ORF clone, Bos taurus(cattle)

The following TRPM8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPM8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa232961 XM_024989669.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa232960 NM_001206066.2
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa232960 XM_005204960.4
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa18217 NM_001206066.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa18217 XM_005204960.3
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 8 (TRPM8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa232961
    Clone ID Related Accession (Same CDS sequence) XM_024989669.1
    Accession Version XM_024989669.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 8 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGTTCA TCGGGGAGGT GGTGAGAGAC AACACCATTA GCAGGAATTC GGAGGAGAAC 
    ATTGTGGCCA TTGGCATCGC CGCTTGGGGC ATGGTCTCCA ACAGGGACAC CCTTATCAGG
    AATTGTGATG TGGAGGGATA TTTTTCAGCT CAGTACATTA TGGATGACTT CAAGAGGGAC
    CCTCTGTACA TCCTGGACAA CAACCACACC CACCTACTGC TCGTGGACAA CGGCTGCCAC
    GGGCACCCCA CAGTGGAGGC AAAGCTCCGG AATCAGCTGG AGAAGTACAT CTCTGAGCGC
    ACGATTCAAG ATTCCAACTA CGGTGGCAAG ATCCCCATTG TGTGTTTTGC CCAAGGAGGT
    GGGAAAGAGA CTTTGAAAGC CATCAATACC TCCGTCAAAA GTAAGATCCC CTGCGTGGTG
    GTGGAAGGCT CGGGGCAGAT CGCCGATGTG ATCGCGAGCC TGGTGGAGGT GGAGGATGTC
    CTGACGTCAT CCATCATCAA GGAGAAGCTG GTGCGCTTCT TGCCCCGCAC GGTGTCCCGG
    CTGCCTGAGG AGGAGACCGA GAGTTGGATC AGATGGCTCA AAGAAATTGT CGAAAGTTCT
    CACCTATTAA CAGTTATTAA AATGGAAGAA GCAGGGGATG AAATTGTGAG CAATGCGATC
    TCCTATGCGT TGTACAAAGC CTTTAGCACC AATGAACAAG ATAAGGATAA CTGGAATGGG
    CAGCTGAAGC TTCTGCTGGA GTGGAACCAG CTGGACATAG CCAATGATGA GATTTTCACC
    AATGACCGCC GATGGGAGTC TGCTGACCTT CAGGAAGTCA TGTTTACAGC TCTTATAAAG
    GACAGACCCA AGTTTGTCCG CCTCTTTCTG GAGAATGGCT TGAACCTGCG GAAGTTCCTC
    ACCAATGATG TCCTCACGGA GCTCTTCTCC AATCACTTCA GCTCCCTGGT GTATCGAAAC
    CTGCAGATCG CCAAGAATTC CTATAATGAT GCCCTCCTCA CCTTTGTCTG GAAACTGGTT
    GCTAACTTCC GAAGAGGCTT CCGGAAGGAA GACAGGAATA GCAGAGATGA AATAGACATA
    GAGCTCCATG ATGTGTCTCC TATTACTCGG CACCCCCTGC AAGCGCTCTT CATCTGGGCC
    ATTCTTCAGA ACAAGAAGGA ACTCTCCAAA GTCATTTGGG AGCAGACCAG GGGCTGTACT
    TTGGCCGCCC TGGGGGCCAG CAAACTTCTG AAGACTCTGG CTAAGGTGAA GAATGACATC
    AACGCTGCAG GGGAGTCGGA GGAGCTGGCA AACGAGTACG AGACCCGGGC AGTGGAGCTG
    TTCACAGAGT GTTACAGCAG CGATGAGGAC TTGGCGGAAC AACTCCTGGT CTATTCCTGT
    GAAGCCTGGG GGGGAAGCAG CTGCTTGGAG CTGGCAGTGG AGGCAACCGA CCAGCATTTC
    ATTGCCCAGC CCGGGGTCCA GAATTTTCTT TCCAAGCAGT GGTATGGAGA GATTTCCCGA
    GACACCAAGA ACTGGAAGAT TATCCTGTGT CTGTTTATTA TACCCTTGGT TGGCTGTGGC
    TTTGTATCAT TTAGGAAGAA GCCCCTGGAC AAGCACAGGA GGCTCCTGTG GTACTACGTG
    GCCTTCTTCA CCTCCCCGTT CGTGGTCTTC TCCTGGAACG TGGTCTTCTA CATCGCCTTC
    CTCCTGCTCT TCGCCTACGT GCTGCTCATG GATTTCCACT CTGTGCCCCA CGCCCCAGAG
    CTCGTCCTCT ACGCGCTCGT CTTTGTCCTG TTCTGTGATG AAGTGAGACA GTGGTGCGTG
    AACGGCGTGA ATTACTTTAC CGACCTGTGG AACGTCATGG ATTCACTCGG GCTTTTCTAC
    TTCATAGCGG GGATTGTCTT TCGGCTCCAC CCTGCTAACA AAACCTCACT GTACTCGGGA
    CGAGTGATTT TTTGCCTGGA TTACATTATA TTCACCCTAA GGTTGATTCA CATTTTCACG
    GTCAGCAGAA ATTTAGGACC CAAGATTATA ATGTTGCAGA GGATGCTGAT CGACGTGTTC
    TTCTTCCTGT TCCTCTTTGC CGTGTGGATG GTGGCCTTTG GTGTGGCCAG GCAGGGCATC
    CTTAGACAGA ACGAGCATCG CTGGAGGTGG ATCTTCCGCT CAGTCATCTA TGAGCCCTAC
    CTGGCCATGT TTGGCCAAGT GCCCAGCGAT GTGGATGGTA CCACGTACGA CTTCTCCCAC
    TGCACCTTCA CTGGGAACGA GTCCAAGCCC CTGTGCGTGG AGCTGGACGA GCACAACCTG
    CCCCGCTTCC CCGAGTGGAT CACCATCCCC CTGGTGTGCA TCTACATGCT GTCCACCAAC
    ATCCTGCTGG TCAATCTGCT GGTCGCCATG TTCGGCTACA CCGTGGGCAC CGTCCAGGAA
    AACAACGACC AGGTCTGGAA GTTCCAGAGG TACTTCCTGG TGCAGGAATA CTGCAGCCGC
    CTCAACATCC CCTTCCCCTT CGTGGTCTTC GCCTACTTCT ACAAGGTGGC CAAGAAGTGC
    TTCCGGTGCT GCTGCAAAGA GAGGAGCACG GAGCCCTCCG CCTGCTGTTT CAGAAATGAA
    GACAACATGA CTCTGGCATG GGAGGGTGTC ATGAAAGAAA ATTACCTTGT CAAGATCAAC
    ACAAAAGCCA GCGACCCGTC AGAGGAAATA AGGCATCGTT TTAGACAGCT GGATACAAAG
    CTTAATGACC TCAAAGGTAT TCTGAAAGAG ATTGCTAATA AAATCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024845437.1
    CDS265..3015
    Translation

    Target ORF information:

    RefSeq Version XM_024989669.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024989669.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGTTCA TCGGGGAGGT GGTGAGAGAC AACACCATTA GCAGGAATTC GGAGGAGAAC 
    ATTGTGGCCA TTGGCATCGC CGCTTGGGGC ATGGTCTCCA ACAGGGACAC CCTTATCAGG
    AATTGTGATG TGGAGGGATA TTTTTCAGCT CAGTACATTA TGGATGACTT CAAGAGGGAC
    CCTCTGTACA TCCTGGACAA CAACCACACC CACCTACTGC TCGTGGACAA CGGCTGCCAC
    GGGCACCCCA CAGTGGAGGC AAAGCTCCGG AATCAGCTGG AGAAGTACAT CTCTGAGCGC
    ACGATTCAAG ATTCCAACTA CGGTGGCAAG ATCCCCATTG TGTGTTTTGC CCAAGGAGGT
    GGGAAAGAGA CTTTGAAAGC CATCAATACC TCCGTCAAAA GTAAGATCCC CTGCGTGGTG
    GTGGAAGGCT CGGGGCAGAT CGCCGATGTG ATCGCGAGCC TGGTGGAGGT GGAGGATGTC
    CTGACGTCAT CCATCATCAA GGAGAAGCTG GTGCGCTTCT TGCCCCGCAC GGTGTCCCGG
    CTGCCTGAGG AGGAGACCGA GAGTTGGATC AGATGGCTCA AAGAAATTGT CGAAAGTTCT
    CACCTATTAA CAGTTATTAA AATGGAAGAA GCAGGGGATG AAATTGTGAG CAATGCGATC
    TCCTATGCGT TGTACAAAGC CTTTAGCACC AATGAACAAG ATAAGGATAA CTGGAATGGG
    CAGCTGAAGC TTCTGCTGGA GTGGAACCAG CTGGACATAG CCAATGATGA GATTTTCACC
    AATGACCGCC GATGGGAGTC TGCTGACCTT CAGGAAGTCA TGTTTACAGC TCTTATAAAG
    GACAGACCCA AGTTTGTCCG CCTCTTTCTG GAGAATGGCT TGAACCTGCG GAAGTTCCTC
    ACCAATGATG TCCTCACGGA GCTCTTCTCC AATCACTTCA GCTCCCTGGT GTATCGAAAC
    CTGCAGATCG CCAAGAATTC CTATAATGAT GCCCTCCTCA CCTTTGTCTG GAAACTGGTT
    GCTAACTTCC GAAGAGGCTT CCGGAAGGAA GACAGGAATA GCAGAGATGA AATAGACATA
    GAGCTCCATG ATGTGTCTCC TATTACTCGG CACCCCCTGC AAGCGCTCTT CATCTGGGCC
    ATTCTTCAGA ACAAGAAGGA ACTCTCCAAA GTCATTTGGG AGCAGACCAG GGGCTGTACT
    TTGGCCGCCC TGGGGGCCAG CAAACTTCTG AAGACTCTGG CTAAGGTGAA GAATGACATC
    AACGCTGCAG GGGAGTCGGA GGAGCTGGCA AACGAGTACG AGACCCGGGC AGTGGAGCTG
    TTCACAGAGT GTTACAGCAG CGATGAGGAC TTGGCGGAAC AACTCCTGGT CTATTCCTGT
    GAAGCCTGGG GGGGAAGCAG CTGCTTGGAG CTGGCAGTGG AGGCAACCGA CCAGCATTTC
    ATTGCCCAGC CCGGGGTCCA GAATTTTCTT TCCAAGCAGT GGTATGGAGA GATTTCCCGA
    GACACCAAGA ACTGGAAGAT TATCCTGTGT CTGTTTATTA TACCCTTGGT TGGCTGTGGC
    TTTGTATCAT TTAGGAAGAA GCCCCTGGAC AAGCACAGGA GGCTCCTGTG GTACTACGTG
    GCCTTCTTCA CCTCCCCGTT CGTGGTCTTC TCCTGGAACG TGGTCTTCTA CATCGCCTTC
    CTCCTGCTCT TCGCCTACGT GCTGCTCATG GATTTCCACT CTGTGCCCCA CGCCCCAGAG
    CTCGTCCTCT ACGCGCTCGT CTTTGTCCTG TTCTGTGATG AAGTGAGACA GTGGTGCGTG
    AACGGCGTGA ATTACTTTAC CGACCTGTGG AACGTCATGG ATTCACTCGG GCTTTTCTAC
    TTCATAGCGG GGATTGTCTT TCGGCTCCAC CCTGCTAACA AAACCTCACT GTACTCGGGA
    CGAGTGATTT TTTGCCTGGA TTACATTATA TTCACCCTAA GGTTGATTCA CATTTTCACG
    GTCAGCAGAA ATTTAGGACC CAAGATTATA ATGTTGCAGA GGATGCTGAT CGACGTGTTC
    TTCTTCCTGT TCCTCTTTGC CGTGTGGATG GTGGCCTTTG GTGTGGCCAG GCAGGGCATC
    CTTAGACAGA ACGAGCATCG CTGGAGGTGG ATCTTCCGCT CAGTCATCTA TGAGCCCTAC
    CTGGCCATGT TTGGCCAAGT GCCCAGCGAT GTGGATGGTA CCACGTACGA CTTCTCCCAC
    TGCACCTTCA CTGGGAACGA GTCCAAGCCC CTGTGCGTGG AGCTGGACGA GCACAACCTG
    CCCCGCTTCC CCGAGTGGAT CACCATCCCC CTGGTGTGCA TCTACATGCT GTCCACCAAC
    ATCCTGCTGG TCAATCTGCT GGTCGCCATG TTCGGCTACA CCGTGGGCAC CGTCCAGGAA
    AACAACGACC AGGTCTGGAA GTTCCAGAGG TACTTCCTGG TGCAGGAATA CTGCAGCCGC
    CTCAACATCC CCTTCCCCTT CGTGGTCTTC GCCTACTTCT ACAAGGTGGC CAAGAAGTGC
    TTCCGGTGCT GCTGCAAAGA GAGGAGCACG GAGCCCTCCG CCTGCTGTTT CAGAAATGAA
    GACAACATGA CTCTGGCATG GGAGGGTGTC ATGAAAGAAA ATTACCTTGT CAAGATCAAC
    ACAAAAGCCA GCGACCCGTC AGAGGAAATA AGGCATCGTT TTAGACAGCT GGATACAAAG
    CTTAATGACC TCAAAGGTAT TCTGAAAGAG ATTGCTAATA AAATCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232960
    Clone ID Related Accession (Same CDS sequence) XM_005204960.4 , NM_001206066.2
    Accession Version NM_001206066.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 8
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000003.1. On May 29, 2018 this sequence version replaced NM_001206066.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
    ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC
    TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT
    TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC
    ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC
    GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG
    TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA
    ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC
    ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC
    ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC
    ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC
    TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC
    ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA
    CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG
    CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC
    ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC
    AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG
    AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC
    TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG
    CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG
    GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA
    CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC
    ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA
    GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT
    GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC
    TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC
    CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG
    AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC
    CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT
    TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT
    CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG
    TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCG
    GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA
    GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG
    CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT
    ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC
    AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG
    AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC
    CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT
    GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC
    ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT
    AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC
    CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG
    CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC
    TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC
    CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT
    GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC
    GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG
    TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC
    TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC
    CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC
    TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC
    TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA
    GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT
    CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT
    AATAAAATCA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192995.1
    CDS1..3315
    Translation

    Target ORF information:

    RefSeq Version NM_001206066.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206066.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
    ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC
    TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT
    TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC
    ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC
    GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG
    TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA
    ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC
    ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC
    ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC
    ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC
    TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC
    ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA
    CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG
    CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC
    ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC
    AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG
    AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC
    TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG
    CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG
    GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA
    CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC
    ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA
    GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT
    GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC
    TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC
    CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG
    AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC
    CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT
    TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT
    CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG
    TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCG
    GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA
    GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG
    CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT
    ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC
    AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG
    AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC
    CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT
    GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC
    ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT
    AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC
    CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG
    CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC
    TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC
    CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT
    GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC
    GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG
    TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC
    TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC
    CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC
    TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC
    TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA
    GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT
    CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT
    AATAAAATCA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232960
    Clone ID Related Accession (Same CDS sequence) XM_005204960.4 , NM_001206066.2
    Accession Version XM_005204960.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204960.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
    ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC
    TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT
    TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC
    ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC
    GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG
    TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA
    ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC
    ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC
    ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC
    ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC
    TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC
    ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA
    CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG
    CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC
    ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC
    AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG
    AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC
    TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG
    CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG
    GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA
    CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC
    ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA
    GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT
    GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC
    TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC
    CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG
    AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC
    CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT
    TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT
    CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG
    TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCG
    GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA
    GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG
    CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT
    ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC
    AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG
    AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC
    CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT
    GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC
    ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT
    AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC
    CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG
    CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC
    TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC
    CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT
    GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC
    GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG
    TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC
    TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC
    CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC
    TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC
    TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA
    GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT
    CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT
    AATAAAATCA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205017.1
    CDS1..3315
    Translation

    Target ORF information:

    RefSeq Version XM_005204960.4
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204960.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
    ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC
    TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT
    TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC
    ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC
    GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG
    TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA
    ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC
    ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC
    ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC
    ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC
    TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC
    ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA
    CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG
    CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC
    ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC
    AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG
    AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC
    TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG
    CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG
    GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA
    CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC
    ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA
    GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT
    GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC
    TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC
    CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG
    AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC
    CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT
    TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT
    CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG
    TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCG
    GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA
    GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG
    CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT
    ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC
    AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG
    AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC
    CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT
    GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC
    ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT
    AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC
    CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG
    CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC
    TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC
    CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT
    GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC
    GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG
    TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC
    TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC
    CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC
    TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC
    TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA
    GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT
    CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT
    AATAAAATCA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18217
    Clone ID Related Accession (Same CDS sequence) XM_005204960.3 , NM_001206066.1
    Accession Version NM_001206066.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 8
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02009282.1. On Apr 20, 2011 this sequence version replaced XM_614767.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120238, SAMN02403640 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
    ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC
    TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT
    TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC
    ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC
    GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG
    TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA
    ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC
    ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC
    ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC
    ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC
    TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC
    ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA
    CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG
    CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC
    ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC
    AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG
    AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC
    TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG
    CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG
    GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA
    CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC
    ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA
    GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT
    GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC
    TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC
    CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG
    AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC
    CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT
    TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT
    CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG
    TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCA
    GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA
    GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG
    CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT
    ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC
    AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG
    AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC
    CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT
    GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC
    ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT
    AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC
    CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG
    CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC
    TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC
    CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT
    GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC
    GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG
    TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC
    TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC
    CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC
    TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC
    TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA
    GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT
    CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT
    AATAAAATCA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192995.1
    CDS1..3315
    Translation

    Target ORF information:

    RefSeq Version NM_001206066.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206066.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
    ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC
    TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT
    TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC
    ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC
    GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG
    TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA
    ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC
    ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC
    ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC
    ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC
    TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC
    ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA
    CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG
    CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC
    ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC
    AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG
    AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC
    TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG
    CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG
    GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA
    CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC
    ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA
    GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT
    GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC
    TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC
    CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG
    AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC
    CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT
    TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT
    CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG
    TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCA
    GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA
    GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG
    CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT
    ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC
    AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG
    AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC
    CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT
    GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC
    ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT
    AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC
    CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG
    CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC
    TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC
    CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT
    GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC
    GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG
    TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC
    TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC
    CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC
    TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC
    TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA
    GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT
    CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT
    AATAAAATCA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18217
    Clone ID Related Accession (Same CDS sequence) XM_005204960.3 , NM_001206066.1
    Accession Version XM_005204960.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103875.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204960.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
    ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC
    TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT
    TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC
    ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC
    GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG
    TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA
    ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC
    ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC
    ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC
    ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC
    TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC
    ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA
    CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG
    CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC
    ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC
    AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG
    AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC
    TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG
    CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG
    GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA
    CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC
    ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA
    GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT
    GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC
    TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC
    CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG
    AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC
    CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT
    TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT
    CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG
    TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCA
    GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA
    GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG
    CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT
    ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC
    AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG
    AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC
    CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT
    GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC
    ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT
    AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC
    CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG
    CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC
    TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC
    CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT
    GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC
    GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG
    TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC
    TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC
    CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC
    TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC
    TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA
    GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT
    CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT
    AATAAAATCA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205017.1
    CDS1..3315
    Translation

    Target ORF information:

    RefSeq Version XM_005204960.3
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 8 (TRPM8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005204960.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
    ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC
    TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT
    TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC
    ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC
    GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG
    TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA
    ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC
    ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC
    ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC
    ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC
    TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC
    ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA
    CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG
    CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC
    ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC
    AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG
    AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC
    TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG
    CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG
    GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA
    CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC
    ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA
    GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT
    GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC
    TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC
    CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG
    AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC
    CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT
    TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT
    CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG
    TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCA
    GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA
    GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG
    CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT
    ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC
    AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG
    AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC
    CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT
    GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC
    ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT
    AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC
    CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG
    CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC
    TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC
    CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT
    GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC
    GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG
    TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC
    TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC
    CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC
    TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC
    TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA
    GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT
    CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT
    AATAAAATCA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.