TRPM8 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRPM8
Entrez Gene ID	541151
Full Name	transient receptor potential cation channel subfamily M member 8
General protein information	Preferred Names transient receptor potential cation channel subfamily M member 8 Names transient receptor potential cation channel subfamily M member 8
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005204960.4	XP_005205017.1	transient receptor potential cation channel subfamily M member 8 isoform X1
XM_005204960.3	XP_005205017.1	transient receptor potential cation channel subfamily M member 8 isoform X1
NM_001206066.2	NP_001192995.1	transient receptor potential cation channel subfamily M member 8
XM_024989669.1	XP_024845437.1	transient receptor potential cation channel subfamily M member 8 isoform X2
NM_001206066.1	NP_001192995.1	transient receptor potential cation channel subfamily M member 8

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	trpm8	NP_001155105.1
Homo sapiens (human)	TRPM8	NP_076985.4
Pan troglodytes (chimpanzee)	TRPM8	XP_003309583.1
Mus musculus (house mouse)	Trpm8	NP_599013.1
Rattus norvegicus (Norway rat)	Trpm8	NP_599198.2
	LOC100429414	XP_002799123.1
Bos taurus (cattle)	TRPM8	NP_001192995.1
Gallus gallus (chicken)	TRPM8	NP_001007083.1
Canis lupus familiaris (dog)	TRPM8	NP_001104239.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TRPM8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPM8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa232961	XM_024989669.1 Latest version!	Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa232960	NM_001206066.2 Latest version!	Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa232960	XM_005204960.4 Latest version!	Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa18217	NM_001206066.1 Latest version!	Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa18217	XM_005204960.3 Latest version!	Bos taurus transient receptor potential cation channel, subfamily M, member 8 (TRPM8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRPM8 ( XM_024989669.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845437.1 Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa232961
Clone ID Related Accession (Same CDS sequence)	XM_024989669.1
Accession Version	XM_024989669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transient receptor potential cation channel subfamily M member 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTCA TCGGGGAGGT GGTGAGAGAC AACACCATTA GCAGGAATTC GGAGGAGAAC 
ATTGTGGCCA TTGGCATCGC CGCTTGGGGC ATGGTCTCCA ACAGGGACAC CCTTATCAGG 
AATTGTGATG TGGAGGGATA TTTTTCAGCT CAGTACATTA TGGATGACTT CAAGAGGGAC 
CCTCTGTACA TCCTGGACAA CAACCACACC CACCTACTGC TCGTGGACAA CGGCTGCCAC 
GGGCACCCCA CAGTGGAGGC AAAGCTCCGG AATCAGCTGG AGAAGTACAT CTCTGAGCGC 
ACGATTCAAG ATTCCAACTA CGGTGGCAAG ATCCCCATTG TGTGTTTTGC CCAAGGAGGT 
GGGAAAGAGA CTTTGAAAGC CATCAATACC TCCGTCAAAA GTAAGATCCC CTGCGTGGTG 
GTGGAAGGCT CGGGGCAGAT CGCCGATGTG ATCGCGAGCC TGGTGGAGGT GGAGGATGTC 
CTGACGTCAT CCATCATCAA GGAGAAGCTG GTGCGCTTCT TGCCCCGCAC GGTGTCCCGG 
CTGCCTGAGG AGGAGACCGA GAGTTGGATC AGATGGCTCA AAGAAATTGT CGAAAGTTCT 
CACCTATTAA CAGTTATTAA AATGGAAGAA GCAGGGGATG AAATTGTGAG CAATGCGATC 
TCCTATGCGT TGTACAAAGC CTTTAGCACC AATGAACAAG ATAAGGATAA CTGGAATGGG 
CAGCTGAAGC TTCTGCTGGA GTGGAACCAG CTGGACATAG CCAATGATGA GATTTTCACC 
AATGACCGCC GATGGGAGTC TGCTGACCTT CAGGAAGTCA TGTTTACAGC TCTTATAAAG 
GACAGACCCA AGTTTGTCCG CCTCTTTCTG GAGAATGGCT TGAACCTGCG GAAGTTCCTC 
ACCAATGATG TCCTCACGGA GCTCTTCTCC AATCACTTCA GCTCCCTGGT GTATCGAAAC 
CTGCAGATCG CCAAGAATTC CTATAATGAT GCCCTCCTCA CCTTTGTCTG GAAACTGGTT 
GCTAACTTCC GAAGAGGCTT CCGGAAGGAA GACAGGAATA GCAGAGATGA AATAGACATA 
GAGCTCCATG ATGTGTCTCC TATTACTCGG CACCCCCTGC AAGCGCTCTT CATCTGGGCC 
ATTCTTCAGA ACAAGAAGGA ACTCTCCAAA GTCATTTGGG AGCAGACCAG GGGCTGTACT 
TTGGCCGCCC TGGGGGCCAG CAAACTTCTG AAGACTCTGG CTAAGGTGAA GAATGACATC 
AACGCTGCAG GGGAGTCGGA GGAGCTGGCA AACGAGTACG AGACCCGGGC AGTGGAGCTG 
TTCACAGAGT GTTACAGCAG CGATGAGGAC TTGGCGGAAC AACTCCTGGT CTATTCCTGT 
GAAGCCTGGG GGGGAAGCAG CTGCTTGGAG CTGGCAGTGG AGGCAACCGA CCAGCATTTC 
ATTGCCCAGC CCGGGGTCCA GAATTTTCTT TCCAAGCAGT GGTATGGAGA GATTTCCCGA 
GACACCAAGA ACTGGAAGAT TATCCTGTGT CTGTTTATTA TACCCTTGGT TGGCTGTGGC 
TTTGTATCAT TTAGGAAGAA GCCCCTGGAC AAGCACAGGA GGCTCCTGTG GTACTACGTG 
GCCTTCTTCA CCTCCCCGTT CGTGGTCTTC TCCTGGAACG TGGTCTTCTA CATCGCCTTC 
CTCCTGCTCT TCGCCTACGT GCTGCTCATG GATTTCCACT CTGTGCCCCA CGCCCCAGAG 
CTCGTCCTCT ACGCGCTCGT CTTTGTCCTG TTCTGTGATG AAGTGAGACA GTGGTGCGTG 
AACGGCGTGA ATTACTTTAC CGACCTGTGG AACGTCATGG ATTCACTCGG GCTTTTCTAC 
TTCATAGCGG GGATTGTCTT TCGGCTCCAC CCTGCTAACA AAACCTCACT GTACTCGGGA 
CGAGTGATTT TTTGCCTGGA TTACATTATA TTCACCCTAA GGTTGATTCA CATTTTCACG 
GTCAGCAGAA ATTTAGGACC CAAGATTATA ATGTTGCAGA GGATGCTGAT CGACGTGTTC 
TTCTTCCTGT TCCTCTTTGC CGTGTGGATG GTGGCCTTTG GTGTGGCCAG GCAGGGCATC 
CTTAGACAGA ACGAGCATCG CTGGAGGTGG ATCTTCCGCT CAGTCATCTA TGAGCCCTAC 
CTGGCCATGT TTGGCCAAGT GCCCAGCGAT GTGGATGGTA CCACGTACGA CTTCTCCCAC 
TGCACCTTCA CTGGGAACGA GTCCAAGCCC CTGTGCGTGG AGCTGGACGA GCACAACCTG 
CCCCGCTTCC CCGAGTGGAT CACCATCCCC CTGGTGTGCA TCTACATGCT GTCCACCAAC 
ATCCTGCTGG TCAATCTGCT GGTCGCCATG TTCGGCTACA CCGTGGGCAC CGTCCAGGAA 
AACAACGACC AGGTCTGGAA GTTCCAGAGG TACTTCCTGG TGCAGGAATA CTGCAGCCGC 
CTCAACATCC CCTTCCCCTT CGTGGTCTTC GCCTACTTCT ACAAGGTGGC CAAGAAGTGC 
TTCCGGTGCT GCTGCAAAGA GAGGAGCACG GAGCCCTCCG CCTGCTGTTT CAGAAATGAA 
GACAACATGA CTCTGGCATG GGAGGGTGTC ATGAAAGAAA ATTACCTTGT CAAGATCAAC 
ACAAAAGCCA GCGACCCGTC AGAGGAAATA AGGCATCGTT TTAGACAGCT GGATACAAAG 
CTTAATGACC TCAAAGGTAT TCTGAAAGAG ATTGCTAATA AAATCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845437.1
CDS	265..3015
Translation	MKFIGEVVRDNTISRNSEENIVAIGIAAWGMVSNRDTLIRNCDVEGYFSAQYIMDDFKRDPLYILDNNHTHLLLVDNGCHGHPTVEAKLRNQLEKYISERTIQDSNYGGKIPIVCFAQGGGKETLKAINTSVKSKIPCVVVEGSGQIADVIASLVEVEDVLTSSIIKEKLVRFLPRTVSRLPEEETESWIRWLKEIVESSHLLTVIKMEEAGDEIVSNAISYALYKAFSTNEQDKDNWNGQLKLLLEWNQLDIANDEIFTNDRRWESADLQEVMFTALIKDRPKFVRLFLENGLNLRKFLTNDVLTELFSNHFSSLVYRNLQIAKNSYNDALLTFVWKLVANFRRGFRKEDRNSRDEIDIELHDVSPITRHPLQALFIWAILQNKKELSKVIWEQTRGCTLAALGASKLLKTLAKVKNDINAAGESEELANEYETRAVELFTECYSSDEDLAEQLLVYSCEAWGGSSCLELAVEATDQHFIAQPGVQNFLSKQWYGEISRDTKNWKIILCLFIIPLVGCGFVSFRKKPLDKHRRLLWYYVAFFTSPFVVFSWNVVFYIAFLLLFAYVLLMDFHSVPHAPELVLYALVFVLFCDEVRQWCVNGVNYFTDLWNVMDSLGLFYFIAGIVFRLHPANKTSLYSGRVIFCLDYIIFTLRLIHIFTVSRNLGPKIIMLQRMLIDVFFFLFLFAVWMVAFGVARQGILRQNEHRWRWIFRSVIYEPYLAMFGQVPSDVDGTTYDFSHCTFTGNESKPLCVELDEHNLPRFPEWITIPLVCIYMLSTNILLVNLLVAMFGYTVGTVQENNDQVWKFQRYFLVQEYCSRLNIPFPFVVFAYFYKVAKKCFRCCCKERSTEPSACCFRNEDNMTLAWEGVMKENYLVKINTKASDPSEEIRHRFRQLDTKLNDLKGILKEIANKIK

Target ORF information:

RefSeq Version	XM_024989669.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989669.1

ORF Insert Sequence:

ATGAAGTTCA TCGGGGAGGT GGTGAGAGAC AACACCATTA GCAGGAATTC GGAGGAGAAC 
ATTGTGGCCA TTGGCATCGC CGCTTGGGGC ATGGTCTCCA ACAGGGACAC CCTTATCAGG 
AATTGTGATG TGGAGGGATA TTTTTCAGCT CAGTACATTA TGGATGACTT CAAGAGGGAC 
CCTCTGTACA TCCTGGACAA CAACCACACC CACCTACTGC TCGTGGACAA CGGCTGCCAC 
GGGCACCCCA CAGTGGAGGC AAAGCTCCGG AATCAGCTGG AGAAGTACAT CTCTGAGCGC 
ACGATTCAAG ATTCCAACTA CGGTGGCAAG ATCCCCATTG TGTGTTTTGC CCAAGGAGGT 
GGGAAAGAGA CTTTGAAAGC CATCAATACC TCCGTCAAAA GTAAGATCCC CTGCGTGGTG 
GTGGAAGGCT CGGGGCAGAT CGCCGATGTG ATCGCGAGCC TGGTGGAGGT GGAGGATGTC 
CTGACGTCAT CCATCATCAA GGAGAAGCTG GTGCGCTTCT TGCCCCGCAC GGTGTCCCGG 
CTGCCTGAGG AGGAGACCGA GAGTTGGATC AGATGGCTCA AAGAAATTGT CGAAAGTTCT 
CACCTATTAA CAGTTATTAA AATGGAAGAA GCAGGGGATG AAATTGTGAG CAATGCGATC 
TCCTATGCGT TGTACAAAGC CTTTAGCACC AATGAACAAG ATAAGGATAA CTGGAATGGG 
CAGCTGAAGC TTCTGCTGGA GTGGAACCAG CTGGACATAG CCAATGATGA GATTTTCACC 
AATGACCGCC GATGGGAGTC TGCTGACCTT CAGGAAGTCA TGTTTACAGC TCTTATAAAG 
GACAGACCCA AGTTTGTCCG CCTCTTTCTG GAGAATGGCT TGAACCTGCG GAAGTTCCTC 
ACCAATGATG TCCTCACGGA GCTCTTCTCC AATCACTTCA GCTCCCTGGT GTATCGAAAC 
CTGCAGATCG CCAAGAATTC CTATAATGAT GCCCTCCTCA CCTTTGTCTG GAAACTGGTT 
GCTAACTTCC GAAGAGGCTT CCGGAAGGAA GACAGGAATA GCAGAGATGA AATAGACATA 
GAGCTCCATG ATGTGTCTCC TATTACTCGG CACCCCCTGC AAGCGCTCTT CATCTGGGCC 
ATTCTTCAGA ACAAGAAGGA ACTCTCCAAA GTCATTTGGG AGCAGACCAG GGGCTGTACT 
TTGGCCGCCC TGGGGGCCAG CAAACTTCTG AAGACTCTGG CTAAGGTGAA GAATGACATC 
AACGCTGCAG GGGAGTCGGA GGAGCTGGCA AACGAGTACG AGACCCGGGC AGTGGAGCTG 
TTCACAGAGT GTTACAGCAG CGATGAGGAC TTGGCGGAAC AACTCCTGGT CTATTCCTGT 
GAAGCCTGGG GGGGAAGCAG CTGCTTGGAG CTGGCAGTGG AGGCAACCGA CCAGCATTTC 
ATTGCCCAGC CCGGGGTCCA GAATTTTCTT TCCAAGCAGT GGTATGGAGA GATTTCCCGA 
GACACCAAGA ACTGGAAGAT TATCCTGTGT CTGTTTATTA TACCCTTGGT TGGCTGTGGC 
TTTGTATCAT TTAGGAAGAA GCCCCTGGAC AAGCACAGGA GGCTCCTGTG GTACTACGTG 
GCCTTCTTCA CCTCCCCGTT CGTGGTCTTC TCCTGGAACG TGGTCTTCTA CATCGCCTTC 
CTCCTGCTCT TCGCCTACGT GCTGCTCATG GATTTCCACT CTGTGCCCCA CGCCCCAGAG 
CTCGTCCTCT ACGCGCTCGT CTTTGTCCTG TTCTGTGATG AAGTGAGACA GTGGTGCGTG 
AACGGCGTGA ATTACTTTAC CGACCTGTGG AACGTCATGG ATTCACTCGG GCTTTTCTAC 
TTCATAGCGG GGATTGTCTT TCGGCTCCAC CCTGCTAACA AAACCTCACT GTACTCGGGA 
CGAGTGATTT TTTGCCTGGA TTACATTATA TTCACCCTAA GGTTGATTCA CATTTTCACG 
GTCAGCAGAA ATTTAGGACC CAAGATTATA ATGTTGCAGA GGATGCTGAT CGACGTGTTC 
TTCTTCCTGT TCCTCTTTGC CGTGTGGATG GTGGCCTTTG GTGTGGCCAG GCAGGGCATC 
CTTAGACAGA ACGAGCATCG CTGGAGGTGG ATCTTCCGCT CAGTCATCTA TGAGCCCTAC 
CTGGCCATGT TTGGCCAAGT GCCCAGCGAT GTGGATGGTA CCACGTACGA CTTCTCCCAC 
TGCACCTTCA CTGGGAACGA GTCCAAGCCC CTGTGCGTGG AGCTGGACGA GCACAACCTG 
CCCCGCTTCC CCGAGTGGAT CACCATCCCC CTGGTGTGCA TCTACATGCT GTCCACCAAC 
ATCCTGCTGG TCAATCTGCT GGTCGCCATG TTCGGCTACA CCGTGGGCAC CGTCCAGGAA 
AACAACGACC AGGTCTGGAA GTTCCAGAGG TACTTCCTGG TGCAGGAATA CTGCAGCCGC 
CTCAACATCC CCTTCCCCTT CGTGGTCTTC GCCTACTTCT ACAAGGTGGC CAAGAAGTGC 
TTCCGGTGCT GCTGCAAAGA GAGGAGCACG GAGCCCTCCG CCTGCTGTTT CAGAAATGAA 
GACAACATGA CTCTGGCATG GGAGGGTGTC ATGAAAGAAA ATTACCTTGT CAAGATCAAC 
ACAAAAGCCA GCGACCCGTC AGAGGAAATA AGGCATCGTT TTAGACAGCT GGATACAAAG 
CTTAATGACC TCAAAGGTAT TCTGAAAGAG ATTGCTAATA AAATCAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM8 ( NM_001206066.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192995.1 Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), mRNA.

Price & Availability↑

CloneID	OBa232960
Clone ID Related Accession (Same CDS sequence)	XM_005204960.4 , NM_001206066.2
Accession Version	NM_001206066.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	transient receptor potential cation channel subfamily M member 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000003.1. On May 29, 2018 this sequence version replaced NM_001206066.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC 
TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT 
TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC 
ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC 
GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG 
TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA 
ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC 
ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC 
ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC 
ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC 
TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC 
ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA 
CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG 
CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC 
ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC 
AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG 
AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC 
TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG 
CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG 
GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA 
CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC 
ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA 
GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT 
GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC 
TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC 
CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG 
AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC 
CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT 
TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT 
CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG 
TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCG 
GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA 
GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG 
CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT 
ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC 
AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG 
AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC 
CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT 
GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC 
ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT 
AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC 
CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG 
CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC 
TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC 
CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT 
GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC 
GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG 
TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC 
TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC 
CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC 
TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC 
TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA 
GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT 
CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT 
AATAAAATCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192995.1
CDS	1..3315
Translation	MSFKGARFSMRNRRNGTPDSTRTLYSSASRSTDVSYAESDLVNFIEANFKKRECVFFTKDSKATENVCKCGYAQNQHIEGTQINQNEKWNYKKHTKEFPTDAFGDVQFETQGKKGKYIRLSCDTDAETLYELLTQHWHLKTPNLVISVTGGARNFALKPRMRKIFSRLIYIAQSKGAWILTGGTHYGLMKFIGEVVRDNTISRNSEENIVAIGIAAWGMVSNRDTLIRNCDVEGYFSAQYIMDDFKRDPLYILDNNHTHLLLVDNGCHGHPTVEAKLRNQLEKYISERTIQDSNYGGKIPIVCFAQGGGKETLKAINTSVKSKIPCVVVEGSGQIADVIASLVEVEDVLTSSIIKEKLVRFLPRTVSRLPEEETESWIRWLKEIVESSHLLTVIKMEEAGDEIVSNAISYALYKAFSTNEQDKDNWNGQLKLLLEWNQLDIANDEIFTNDRRWESADLQEVMFTALIKDRPKFVRLFLENGLNLRKFLTNDVLTELFSNHFSSLVYRNLQIAKNSYNDALLTFVWKLVANFRRGFRKEDRNSRDEIDIELHDVSPITRHPLQALFIWAILQNKKELSKVIWEQTRGCTLAALGASKLLKTLAKVKNDINAAGESEELANEYETRAVELFTECYSSDEDLAEQLLVYSCEAWGGSSCLELAVEATDQHFIAQPGVQNFLSKQWYGEISRDTKNWKIILCLFIIPLVGCGFVSFRKKPLDKHRRLLWYYVAFFTSPFVVFSWNVVFYIAFLLLFAYVLLMDFHSVPHAPELVLYALVFVLFCDEVRQWCVNGVNYFTDLWNVMDSLGLFYFIAGIVFRLHPANKTSLYSGRVIFCLDYIIFTLRLIHIFTVSRNLGPKIIMLQRMLIDVFFFLFLFAVWMVAFGVARQGILRQNEHRWRWIFRSVIYEPYLAMFGQVPSDVDGTTYDFSHCTFTGNESKPLCVELDEHNLPRFPEWITIPLVCIYMLSTNILLVNLLVAMFGYTVGTVQENNDQVWKFQRYFLVQEYCSRLNIPFPFVVFAYFYKVAKKCFRCCCKERSTEPSACCFRNEDNMTLAWEGVMKENYLVKINTKASDPSEEIRHRFRQLDTKLNDLKGILKEIANKIK

Target ORF information:

RefSeq Version	NM_001206066.2
Organism	Bos taurus(cattle)
Definition	Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206066.2

ORF Insert Sequence:

ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC 
TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT 
TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC 
ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC 
GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG 
TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA 
ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC 
ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC 
ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC 
ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC 
TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC 
ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA 
CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG 
CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC 
ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC 
AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG 
AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC 
TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG 
CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG 
GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA 
CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC 
ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA 
GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT 
GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC 
TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC 
CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG 
AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC 
CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT 
TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT 
CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG 
TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCG 
GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA 
GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG 
CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT 
ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC 
AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG 
AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC 
CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT 
GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC 
ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT 
AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC 
CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG 
CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC 
TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC 
CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT 
GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC 
GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG 
TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC 
TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC 
CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC 
TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC 
TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA 
GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT 
CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT 
AATAAAATCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM8 ( XM_005204960.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205017.1 Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), transcript variant X1, mRNA.

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CloneID	OBa232960
Clone ID Related Accession (Same CDS sequence)	XM_005204960.4 , NM_001206066.2
Accession Version	XM_005204960.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transient receptor potential cation channel subfamily M member 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204960.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC 
TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT 
TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC 
ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC 
GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG 
TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA 
ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC 
ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC 
ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC 
ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC 
TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC 
ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA 
CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG 
CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC 
ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC 
AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG 
AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC 
TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG 
CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG 
GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA 
CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC 
ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA 
GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT 
GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC 
TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC 
CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG 
AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC 
CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT 
TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT 
CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG 
TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCG 
GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA 
GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG 
CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT 
ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC 
AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG 
AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC 
CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT 
GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC 
ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT 
AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC 
CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG 
CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC 
TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC 
CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT 
GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC 
GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG 
TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC 
TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC 
CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC 
TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC 
TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA 
GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT 
CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT 
AATAAAATCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205017.1
CDS	1..3315
Translation	MSFKGARFSMRNRRNGTPDSTRTLYSSASRSTDVSYAESDLVNFIEANFKKRECVFFTKDSKATENVCKCGYAQNQHIEGTQINQNEKWNYKKHTKEFPTDAFGDVQFETQGKKGKYIRLSCDTDAETLYELLTQHWHLKTPNLVISVTGGARNFALKPRMRKIFSRLIYIAQSKGAWILTGGTHYGLMKFIGEVVRDNTISRNSEENIVAIGIAAWGMVSNRDTLIRNCDVEGYFSAQYIMDDFKRDPLYILDNNHTHLLLVDNGCHGHPTVEAKLRNQLEKYISERTIQDSNYGGKIPIVCFAQGGGKETLKAINTSVKSKIPCVVVEGSGQIADVIASLVEVEDVLTSSIIKEKLVRFLPRTVSRLPEEETESWIRWLKEIVESSHLLTVIKMEEAGDEIVSNAISYALYKAFSTNEQDKDNWNGQLKLLLEWNQLDIANDEIFTNDRRWESADLQEVMFTALIKDRPKFVRLFLENGLNLRKFLTNDVLTELFSNHFSSLVYRNLQIAKNSYNDALLTFVWKLVANFRRGFRKEDRNSRDEIDIELHDVSPITRHPLQALFIWAILQNKKELSKVIWEQTRGCTLAALGASKLLKTLAKVKNDINAAGESEELANEYETRAVELFTECYSSDEDLAEQLLVYSCEAWGGSSCLELAVEATDQHFIAQPGVQNFLSKQWYGEISRDTKNWKIILCLFIIPLVGCGFVSFRKKPLDKHRRLLWYYVAFFTSPFVVFSWNVVFYIAFLLLFAYVLLMDFHSVPHAPELVLYALVFVLFCDEVRQWCVNGVNYFTDLWNVMDSLGLFYFIAGIVFRLHPANKTSLYSGRVIFCLDYIIFTLRLIHIFTVSRNLGPKIIMLQRMLIDVFFFLFLFAVWMVAFGVARQGILRQNEHRWRWIFRSVIYEPYLAMFGQVPSDVDGTTYDFSHCTFTGNESKPLCVELDEHNLPRFPEWITIPLVCIYMLSTNILLVNLLVAMFGYTVGTVQENNDQVWKFQRYFLVQEYCSRLNIPFPFVVFAYFYKVAKKCFRCCCKERSTEPSACCFRNEDNMTLAWEGVMKENYLVKINTKASDPSEEIRHRFRQLDTKLNDLKGILKEIANKIK

Target ORF information:

RefSeq Version	XM_005204960.4
Organism	Bos taurus(cattle)
Definition	Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204960.4

ORF Insert Sequence:

ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC 
TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT 
TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC 
ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC 
GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG 
TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA 
ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC 
ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC 
ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC 
ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC 
TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC 
ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA 
CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG 
CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC 
ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC 
AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG 
AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC 
TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG 
CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG 
GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA 
CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC 
ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA 
GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT 
GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC 
TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC 
CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG 
AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC 
CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT 
TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT 
CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG 
TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCG 
GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA 
GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG 
CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT 
ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC 
AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG 
AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC 
CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT 
GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC 
ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT 
AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC 
CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG 
CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC 
TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC 
CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT 
GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC 
GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG 
TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC 
TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC 
CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC 
TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC 
TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA 
GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT 
CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT 
AATAAAATCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM8 ( NM_001206066.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192995.1 Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), mRNA.

Price & Availability↑

CloneID	OBa18217
Clone ID Related Accession (Same CDS sequence)	XM_005204960.3 , NM_001206066.1
Accession Version	NM_001206066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	transient receptor potential cation channel subfamily M member 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02009282.1. On Apr 20, 2011 this sequence version replaced XM_614767.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120238, SAMN02403640 [ECO:0000350] ##Evidence-Data-END##

ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC 
TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT 
TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC 
ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC 
GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG 
TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA 
ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC 
ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC 
ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC 
ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC 
TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC 
ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA 
CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG 
CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC 
ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC 
AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG 
AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC 
TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG 
CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG 
GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA 
CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC 
ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA 
GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT 
GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC 
TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC 
CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG 
AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC 
CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT 
TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT 
CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG 
TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCA 
GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA 
GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG 
CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT 
ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC 
AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG 
AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC 
CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT 
GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC 
ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT 
AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC 
CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG 
CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC 
TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC 
CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT 
GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC 
GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG 
TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC 
TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC 
CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC 
TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC 
TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA 
GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT 
CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT 
AATAAAATCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192995.1
CDS	1..3315
Translation	MSFKGARFSMRNRRNGTPDSTRTLYSSASRSTDVSYAESDLVNFIEANFKKRECVFFTKDSKATENVCKCGYAQNQHIEGTQINQNEKWNYKKHTKEFPTDAFGDVQFETQGKKGKYIRLSCDTDAETLYELLTQHWHLKTPNLVISVTGGARNFALKPRMRKIFSRLIYIAQSKGAWILTGGTHYGLMKFIGEVVRDNTISRNSEENIVAIGIAAWGMVSNRDTLIRNCDVEGYFSAQYIMDDFKRDPLYILDNNHTHLLLVDNGCHGHPTVEAKLRNQLEKYISERTIQDSNYGGKIPIVCFAQGGGKETLKAINTSVKSKIPCVVVEGSGQIADVIASLVEVEDVLTSSIIKEKLVRFLPRTVSRLPEEETESWIRWLKEIVESSHLLTVIKMEEAGDEIVSNAISYALYKAFSTNEQDKDNWNGQLKLLLEWNQLDIANDEIFTNDRRWESADLQEVMFTALIKDRPKFVRLFLENGLNLRKFLTNDVLTELFSNHFSSLVYRNLQIAKNSYNDALLTFVWKLVANFRRGFRKEDRNSRDEIDIELHDVSPITRHPLQALFIWAILQNKKELSKVIWEQTRGCTLAALGASKLLKTLAKVKNDINAAGESEELANEYETRAVELFTECYSSDEDLAEQLLVYSCEAWGGSSCLELAVEATDQHFIAQPGVQNFLSKQWYGEISRDTKNWKIILCLFIIPLVGCGFVSFRKKPLDKHRRLLWYYVAFFTSPFVVFSWNVVFYIAFLLLFAYVLLMDFHSVPHAPELVLYALVFVLFCDEVRQWCVNGVNYFTDLWNVMDSLGLFYFIAGIVFRLHPANKTSLYSGRVIFCLDYIIFTLRLIHIFTVSRNLGPKIIMLQRMLIDVFFFLFLFAVWMVAFGVARQGILRQNEHRWRWIFRSVIYEPYLAMFGQVPSDVDGTTYDFSHCTFTGNESKPLCVELDEHNLPRFPEWITIPLVCIYMLSTNILLVNLLVAMFGYTVGTVQENNDQVWKFQRYFLVQEYCSRLNIPFPFVVFAYFYKVAKKCFRCCCKERSTEPSACCFRNEDNMTLAWEGVMKENYLVKINTKASDPSEEIRHRFRQLDTKLNDLKGILKEIANKIK

Target ORF information:

RefSeq Version	NM_001206066.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transient receptor potential cation channel subfamily M member 8 (TRPM8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206066.1

ORF Insert Sequence:

ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC 
TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT 
TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC 
ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC 
GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG 
TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA 
ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC 
ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC 
ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC 
ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC 
TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC 
ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA 
CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG 
CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC 
ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC 
AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG 
AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC 
TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG 
CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG 
GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA 
CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC 
ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA 
GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT 
GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC 
TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC 
CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG 
AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC 
CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT 
TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT 
CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG 
TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCA 
GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA 
GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG 
CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT 
ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC 
AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG 
AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC 
CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT 
GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC 
ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT 
AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC 
CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG 
CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC 
TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC 
CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT 
GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC 
GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG 
TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC 
TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC 
CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC 
TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC 
TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA 
GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT 
CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT 
AATAAAATCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM8 ( XM_005204960.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205017.1 Bos taurus transient receptor potential cation channel, subfamily M, member 8 (TRPM8), transcript variant X1, mRNA.

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CloneID	OBa18217
Clone ID Related Accession (Same CDS sequence)	XM_005204960.3 , NM_001206066.1
Accession Version	XM_005204960.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transient receptor potential cation channel subfamily M member 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103875.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204960.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC 
TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT 
TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC 
ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC 
GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG 
TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA 
ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC 
ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC 
ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC 
ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC 
TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC 
ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA 
CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG 
CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC 
ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC 
AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG 
AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC 
TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG 
CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG 
GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA 
CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC 
ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA 
GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT 
GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC 
TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC 
CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG 
AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC 
CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT 
TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT 
CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG 
TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCA 
GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA 
GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG 
CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT 
ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC 
AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG 
AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC 
CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT 
GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC 
ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT 
AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC 
CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG 
CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC 
TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC 
CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT 
GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC 
GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG 
TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC 
TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC 
CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC 
TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC 
TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA 
GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT 
CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT 
AATAAAATCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205017.1
CDS	1..3315
Translation	MSFKGARFSMRNRRNGTPDSTRTLYSSASRSTDVSYAESDLVNFIEANFKKRECVFFTKDSKATENVCKCGYAQNQHIEGTQINQNEKWNYKKHTKEFPTDAFGDVQFETQGKKGKYIRLSCDTDAETLYELLTQHWHLKTPNLVISVTGGARNFALKPRMRKIFSRLIYIAQSKGAWILTGGTHYGLMKFIGEVVRDNTISRNSEENIVAIGIAAWGMVSNRDTLIRNCDVEGYFSAQYIMDDFKRDPLYILDNNHTHLLLVDNGCHGHPTVEAKLRNQLEKYISERTIQDSNYGGKIPIVCFAQGGGKETLKAINTSVKSKIPCVVVEGSGQIADVIASLVEVEDVLTSSIIKEKLVRFLPRTVSRLPEEETESWIRWLKEIVESSHLLTVIKMEEAGDEIVSNAISYALYKAFSTNEQDKDNWNGQLKLLLEWNQLDIANDEIFTNDRRWESADLQEVMFTALIKDRPKFVRLFLENGLNLRKFLTNDVLTELFSNHFSSLVYRNLQIAKNSYNDALLTFVWKLVANFRRGFRKEDRNSRDEIDIELHDVSPITRHPLQALFIWAILQNKKELSKVIWEQTRGCTLAALGASKLLKTLAKVKNDINAAGESEELANEYETRAVELFTECYSSDEDLAEQLLVYSCEAWGGSSCLELAVEATDQHFIAQPGVQNFLSKQWYGEISRDTKNWKIILCLFIIPLVGCGFVSFRKKPLDKHRRLLWYYVAFFTSPFVVFSWNVVFYIAFLLLFAYVLLMDFHSVPHAPELVLYALVFVLFCDEVRQWCVNGVNYFTDLWNVMDSLGLFYFIAGIVFRLHPANKTSLYSGRVIFCLDYIIFTLRLIHIFTVSRNLGPKIIMLQRMLIDVFFFLFLFAVWMVAFGVARQGILRQNEHRWRWIFRSVIYEPYLAMFGQVPSDVDGTTYDFSHCTFTGNESKPLCVELDEHNLPRFPEWITIPLVCIYMLSTNILLVNLLVAMFGYTVGTVQENNDQVWKFQRYFLVQEYCSRLNIPFPFVVFAYFYKVAKKCFRCCCKERSTEPSACCFRNEDNMTLAWEGVMKENYLVKINTKASDPSEEIRHRFRQLDTKLNDLKGILKEIANKIK

Target ORF information:

RefSeq Version	XM_005204960.3
Organism	Bos taurus(cattle)
Definition	Bos taurus transient receptor potential cation channel, subfamily M, member 8 (TRPM8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204960.3

ORF Insert Sequence:

ATGTCCTTCA AGGGTGCACG GTTCAGCATG AGGAACAGAA GGAATGGCAC CCCGGACAGC 
ACCCGGACCC TGTACTCCAG CGCGTCCCGG AGCACCGACG TGTCCTACGC CGAGAGCGAC 
TTGGTGAATT TCATCGAAGC AAATTTTAAG AAACGGGAGT GTGTCTTCTT TACCAAAGAT 
TCCAAGGCCA CGGAGAATGT GTGCAAGTGT GGCTACGCCC AGAACCAACA CATAGAAGGC 
ACCCAGATCA ACCAAAACGA GAAATGGAAT TACAAGAAAC ATACCAAGGA ATTTCCCACC 
GATGCTTTTG GAGATGTTCA GTTTGAGACT CAGGGGAAGA AAGGGAAGTA TATCCGACTG 
TCCTGCGACA CGGACGCGGA GACGCTGTAC GAGCTGCTGA CCCAGCACTG GCACCTGAAA 
ACACCCAACC TGGTCATTTC CGTGACTGGC GGCGCCAGAA ACTTCGCCCT GAAGCCGCGC 
ATGCGCAAGA TCTTCAGCCG CCTGATCTAC ATCGCGCAGT CGAAAGGTGC CTGGATTCTC 
ACTGGAGGCA CCCACTATGG GCTGATGAAG TTCATCGGGG AGGTGGTGAG AGACAACACC 
ATTAGCAGGA ATTCGGAGGA GAACATTGTG GCCATTGGCA TCGCCGCTTG GGGCATGGTC 
TCCAACAGGG ACACCCTTAT CAGGAATTGT GATGTGGAGG GATATTTTTC AGCTCAGTAC 
ATTATGGATG ACTTCAAGAG GGACCCTCTG TACATCCTGG ACAACAACCA CACCCACCTA 
CTGCTCGTGG ACAACGGCTG CCACGGGCAC CCCACAGTGG AGGCAAAGCT CCGGAATCAG 
CTGGAGAAGT ACATCTCTGA GCGCACGATT CAAGATTCCA ACTACGGTGG CAAGATCCCC 
ATTGTGTGTT TTGCCCAAGG AGGTGGGAAA GAGACTTTGA AAGCCATCAA TACCTCCGTC 
AAAAGTAAGA TCCCCTGCGT GGTGGTGGAA GGCTCGGGGC AGATCGCCGA TGTGATCGCG 
AGCCTGGTGG AGGTGGAGGA TGTCCTGACG TCATCCATCA TCAAGGAGAA GCTGGTGCGC 
TTCTTGCCCC GCACGGTGTC CCGGCTGCCT GAGGAGGAGA CCGAGAGTTG GATCAGATGG 
CTCAAAGAAA TTGTCGAAAG TTCTCACCTA TTAACAGTTA TTAAAATGGA AGAAGCAGGG 
GATGAAATTG TGAGCAATGC GATCTCCTAT GCGTTGTACA AAGCCTTTAG CACCAATGAA 
CAAGATAAGG ATAACTGGAA TGGGCAGCTG AAGCTTCTGC TGGAGTGGAA CCAGCTGGAC 
ATAGCCAATG ATGAGATTTT CACCAATGAC CGCCGATGGG AGTCTGCTGA CCTTCAGGAA 
GTCATGTTTA CAGCTCTTAT AAAGGACAGA CCCAAGTTTG TCCGCCTCTT TCTGGAGAAT 
GGCTTGAACC TGCGGAAGTT CCTCACCAAT GATGTCCTCA CGGAGCTCTT CTCCAATCAC 
TTCAGCTCCC TGGTGTATCG AAACCTGCAG ATCGCCAAGA ATTCCTATAA TGATGCCCTC 
CTCACCTTTG TCTGGAAACT GGTTGCTAAC TTCCGAAGAG GCTTCCGGAA GGAAGACAGG 
AATAGCAGAG ATGAAATAGA CATAGAGCTC CATGATGTGT CTCCTATTAC TCGGCACCCC 
CTGCAAGCGC TCTTCATCTG GGCCATTCTT CAGAACAAGA AGGAACTCTC CAAAGTCATT 
TGGGAGCAGA CCAGGGGCTG TACTTTGGCC GCCCTGGGGG CCAGCAAACT TCTGAAGACT 
CTGGCTAAGG TGAAGAATGA CATCAACGCT GCAGGGGAGT CGGAGGAGCT GGCAAACGAG 
TACGAGACCC GGGCAGTGGA GCTGTTCACA GAGTGTTACA GCAGCGATGA GGACTTGGCA 
GAACAACTCC TGGTCTATTC CTGTGAAGCC TGGGGGGGAA GCAGCTGCTT GGAGCTGGCA 
GTGGAGGCAA CCGACCAGCA TTTCATTGCC CAGCCCGGGG TCCAGAATTT TCTTTCCAAG 
CAGTGGTATG GAGAGATTTC CCGAGACACC AAGAACTGGA AGATTATCCT GTGTCTGTTT 
ATTATACCCT TGGTTGGCTG TGGCTTTGTA TCATTTAGGA AGAAGCCCCT GGACAAGCAC 
AGGAGGCTCC TGTGGTACTA CGTGGCCTTC TTCACCTCCC CGTTCGTGGT CTTCTCCTGG 
AACGTGGTCT TCTACATCGC CTTCCTCCTG CTCTTCGCCT ACGTGCTGCT CATGGATTTC 
CACTCTGTGC CCCACGCCCC AGAGCTCGTC CTCTACGCGC TCGTCTTTGT CCTGTTCTGT 
GATGAAGTGA GACAGTGGTG CGTGAACGGC GTGAATTACT TTACCGACCT GTGGAACGTC 
ATGGATTCAC TCGGGCTTTT CTACTTCATA GCGGGGATTG TCTTTCGGCT CCACCCTGCT 
AACAAAACCT CACTGTACTC GGGACGAGTG ATTTTTTGCC TGGATTACAT TATATTCACC 
CTAAGGTTGA TTCACATTTT CACGGTCAGC AGAAATTTAG GACCCAAGAT TATAATGTTG 
CAGAGGATGC TGATCGACGT GTTCTTCTTC CTGTTCCTCT TTGCCGTGTG GATGGTGGCC 
TTTGGTGTGG CCAGGCAGGG CATCCTTAGA CAGAACGAGC ATCGCTGGAG GTGGATCTTC 
CGCTCAGTCA TCTATGAGCC CTACCTGGCC ATGTTTGGCC AAGTGCCCAG CGATGTGGAT 
GGTACCACGT ACGACTTCTC CCACTGCACC TTCACTGGGA ACGAGTCCAA GCCCCTGTGC 
GTGGAGCTGG ACGAGCACAA CCTGCCCCGC TTCCCCGAGT GGATCACCAT CCCCCTGGTG 
TGCATCTACA TGCTGTCCAC CAACATCCTG CTGGTCAATC TGCTGGTCGC CATGTTCGGC 
TACACCGTGG GCACCGTCCA GGAAAACAAC GACCAGGTCT GGAAGTTCCA GAGGTACTTC 
CTGGTGCAGG AATACTGCAG CCGCCTCAAC ATCCCCTTCC CCTTCGTGGT CTTCGCCTAC 
TTCTACAAGG TGGCCAAGAA GTGCTTCCGG TGCTGCTGCA AAGAGAGGAG CACGGAGCCC 
TCCGCCTGCT GTTTCAGAAA TGAAGACAAC ATGACTCTGG CATGGGAGGG TGTCATGAAA 
GAAAATTACC TTGTCAAGAT CAACACAAAA GCCAGCGACC CGTCAGAGGA AATAAGGCAT 
CGTTTTAGAC AGCTGGATAC AAAGCTTAAT GACCTCAAAG GTATTCTGAA AGAGATTGCT 
AATAAAATCA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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TRPM8 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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