DHX57 cDNA ORF clone, Bos taurus(cattle)

The following DHX57 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHX57 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa220591 XM_024999077.1
Latest version!
Bos taurus DExH-box helicase 57 (DHX57), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa91874 XM_015473454.1
Latest version!
Bos taurus DEAH-box helicase 57 (DHX57), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa23962 NM_001206051.2
Latest version!
Bos taurus DExH-box helicase 57 (DHX57), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa36966 NM_001206051.3
Latest version!
Bos taurus DExH-box helicase 57 (DHX57), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa23962 XM_005212667.3
Latest version!
Bos taurus DEAH-box helicase 57 (DHX57), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa23962 XM_005212666.3
Latest version!
Bos taurus DEAH-box helicase 57 (DHX57), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa36966 XM_005212667.4
Latest version!
Bos taurus DExH-box helicase 57 (DHX57), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa36966 XM_005212666.4
Latest version!
Bos taurus DExH-box helicase 57 (DHX57), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa36966 NM_001206051.1 Bos taurus DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57 (DHX57), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa220591
    Clone ID Related Accession (Same CDS sequence) XM_024999077.1
    Accession Version XM_024999077.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative ATP-dependent RNA helicase DHX57 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAGGTGTTTC TGGGGAAGAA GAAGATGATG AGCCTGATTA TGACGACGAG
    CAGTACTGGT CACCTGGACG AGAAGCTTCC CTTGTTCCTG ACTCTGATCC TTTGGAATGC
    GCTGGCTCAG GCCTGGTGGA GCCTCATAAC CCAGAATTTA CAGTCTCAGC ATTTGCAGTG
    CACAGACTTT CCAGGTATGG TTTCCACACT GAACACTGTC AGGCAGCGCT GAGGATTTGC
    GATGGAGACA TGGGGGCATC ACTGGAACGC CTGCTCACCC AGTGCTTTTC AGAGACATTT
    GGAGAGAGGA TGAAGATATC TGAGGCAGTT GCACACATGA GCTTGGAAGA GTGTGTGGAA
    CAGCGACAGG AAGAGGCATT TGCTCTCAAG TCAATCTGTG GAGAAAAATT TATAGAAAGA
    ATTCAAAACA GAGTCTGGAC CATTGGATTA GAACTGGAGT ATTTGACAAG TAAATTCTGC
    AAATTCAAGC AAAAGGAAAG TACCAAAAAT ATACAGGAGG CTTCACTTGA AGTCTGCAAA
    TTTTACCTCA AAGGAAATTG TAAATTTGGA TCAAAGTGCA AATTTAAACA TGAAGTGCCC
    CCAAATCAAA TTGTCGGAAG AGTCGAAAGA AATGTAGATG ATTCTCATCT AAATGCTAAT
    GAAGATGCAT CCTTTTTATA TGAACTTGAA ATTCGATTTT CTAAAGACCA CAAATACCCC
    TACCAAGCTC CCCTTGTGGC ATTTTATTCC ACCAATGAGA ATCTACCTCT GGCTTGTCGT
    TTACATATTT CTGAGTTCCT TTATGGCAAA GCCTTGACAT TTGCAGAAAC TTCAGAACCT
    GTTGTATATT CTTTGATAAC CCTCTTAGAG GAAGAATCAG AAATAACCAA GTTACTAACA
    AATACCCATC ACAAGTACAG TGTCCCTCCT GTGAACTTTC TGCCAGTATC ACCTGGAACC
    AGACTAAATA ATCCTGTCTG TCGTAAACCA GTGATTCCAA GTAATTCGTT TTTTTCGAAT
    CAAATTCCAG AAGTTGAAAA AGAATCAGAG CCTGAGGAGG AGACAGATGA GGATGAAGGT
    CCAGCACCAG TTATGGTGGA GAATGAAAGC TATGTCAACC TTAAGAAAAA GATTTCCAAA
    AGATATGACT GGCAGGCAAA ATCAGTACAT GCTGAAAATG TCAAAATCTG CAAGCAGTTC
    CGAATAAAAC AGGCCTCCAG ACAGTTCCAG TCAGTTCTAC AAGAAAGACA GTCGCTCCCT
    GCTTGGGAAG AAAGAGAAAA CATTCTCAAA TTGTTGAGCA AGCACCAGGT GCTTGTTGTA
    AGTGGTATGA CTGGATGTGG GAAAACCACA CAGATTCCAC AGTTTATTCT GGATGATTCT
    CTGAATGGAC CCCCTGAGAA GGTGGCTAAC ATCATCTGTA CCCAACCCCG ACGAATCTCT
    GCCATCTCTG TTGCTGAGCG CGTTGCTAAA GAAAGGGCAG AAAGAGTGGG TCTGACCGTG
    GGATACCAGA TCCGGTTAGA AAGTGTCAAG TCCTCAGCCA CCAGGTTGTT ATACTGCACC
    ACGGGAGTGC TGCTAAGAAG GCTGGAGGGA GACACAGCTC TGCAGGGAGT CACCCATATC
    ATTGTTGATG AAGTTCACGA GAGGACAGAA GAAAGTGACT TCTTACTACT AGTTTTGAAA
    GACATTGTGT TGCAGAGACC AACTCTTCAA GTTATTCTGA TGAGTGCAAC CTTAAACGCT
    GAGCTCTTTT CAGAATATTT TAATTCCTGC CCAGTTATCA CTATACCAGG TCGTACATTT
    CCTGTTGATC AGTTTTTTCT GGAAGATGCA ATTGCTGTGA CACGGTATGT GTTGCAGGAT
    GGGAGCCCAT ACGCCCGCTC CATGAAACAG ATGTCAAAGG AGAAGCTCAA GGCCAGGCGC
    AGCAGAACCG CATTTGAGGA GGTGGAGGAG GACCTGCGTC TCTCCCTGCA CCTCCAGGAT
    CAGGATTCTG TCAAAGATGC GGTACCAGAT CAACAATTAG ATTTTAAACA GCTCCTTGCC
    CGCTATCAAG GAGTTAGCAA ATCAGTCATC AAAACAATGT CCGTTATGGA TTTTGAAAAG
    GTTAATCTTG AATTAATCGA GGCCTTGTTA GAGTGGATTG TGGACGGAAA GCACTCCTAC
    CCTCCAGGTG CTATACTTGT ATTTTTACCG GGACTAGCAG AAATCAAAAT GCTGTATGAA
    CAGCTCCAGT CTAATTCTCT TTTCAACAAT AGACGTAGTA ATCGATGTGT TATTCACCCA
    CTTCATTCGT CTTTATCCAG TGAAGAGCAG CAGGCTGTGT TTGTAAAACC TCCTATGGGT
    GTAACTAAGA TTATAATTTC CACCAATATT GCTGAGACAT CCATAACCAT CGATGATGTT
    GTCTATGTCA TTGATTCTGG GAAAATGAAA GAAAAGAGAT ATGATGCCAG CAAAGGGATG
    GAAAGTTTAG AGGATACTTT TGTCTCTCAA GCCAATGCCC TGCAAAGAAA AGGTCGAGCA
    GGTCGTGTAG CGTCTGGGGT CTGCTTCCAT TTATTCACCA GCCATCACTT TAATCACCAA
    CTTCTAAAGC AGCAGCTACC AGAAATACAA AGAGTGCCAT TGGAACAGCT CTGTCTGAGA
    ATTAAAATTT TAGAGATGTT TAGCACTCAC AATCTCCAGT CTGTGTTCGC TCGGCTCATT
    GAACCCCCAC ACGCTGATTC TCTCCGTGCC TCAAAAATAC GATTACGAGA CTTGGGAGCA
    TTAACTCCAG ACGAAACACT GACCCCTCTA GGGTATCACT TGGCCTCTTT GCCTGTAGAT
    GTGAGAATCG GCAAACTAAT GCTCTTTGGA TCTATCTTCC GCTGTTTGGA TCCTGCTCTC
    ACCATTGCTG CCAGTTTAGC TTTTAAGTCT CCTTTTGTAT CTCCCTGGGA TAAAAAAGAG
    GAAGCTAACC AGAAAAAGCT AGAATTTGCA TTTGCAAACA GTGATTATCT GGCCCTTCTA
    CGAGCATATA AGGGATGGCA GCTAAGTATG AAAGAAGGTA TGCGTGCAAG TTATAATTAC
    TGCAGACAAA ACTTCTTGTC AGGAAGAGTT CTTCAGGAAA TGGCAAGCCT GAAACGACAG
    TTCACTGAAC TGTTATCGGA CATAGGCTTT GTTAAGGAGG GACTCCGAGC AAGGGAAATT
    GAGAAAAGAG CCCAAGGAGG AGGAGATGGT ATATTGGATG CCACAGGAGA AGAGGCAAAC
    TCAAATGCTG AGAACCCCAA GTTGATATCA GCAATGCTGT GTGCTGCTTT GTATCCAAAT
    GTAGTGCAGG TGAAAAGCCC AGAGGGGAAA TTTCAGAAGA CCAGTACCGG AGCTGTCAGA
    ATGCAGCCAA AATCAGATGA GTTGAAGTTT GTCACCAAGA ACGATGGATA CGTACACATT
    CACCCTTCAT CGGTGAACTA TCAGGTCAGA CACTTTGACA GTCCCTACCT GGTATACCAC
    GAGAAGATCA AAACTAGTCG CGTGTTCATC CGAGACTGCA GCATGGTGTC TGTGTACCCA
    CTGGTCTTAT TTGGAGGAGG CCAAGTGAAT GTGCAGCTCC AAAAGGGAGA GTTTGTTGTT
    TCCTTGGATG ATGGTTGGAT CCGCTTTGCA GCTGCTTCCC ATCAGGTGGC AGAATTAGTA
    AAGGAACTTC GTTGTGAACT TGACCAGCTC CTCCAGGATA AGATAAAAAA CCCAAGCATA
    GATCTGTGCA CCTGTCCTCG AGGGTCACGG ATCATCAGCA TGATTGTGAA ACTTGTCACC
    ACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854845.1
    CDS82..4230
    Translation

    Target ORF information:

    RefSeq Version XM_024999077.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DExH-box helicase 57 (DHX57), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999077.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAGGTGTTTC TGGGGAAGAA GAAGATGATG AGCCTGATTA TGACGACGAG
    CAGTACTGGT CACCTGGACG AGAAGCTTCC CTTGTTCCTG ACTCTGATCC TTTGGAATGC
    GCTGGCTCAG GCCTGGTGGA GCCTCATAAC CCAGAATTTA CAGTCTCAGC ATTTGCAGTG
    CACAGACTTT CCAGGTATGG TTTCCACACT GAACACTGTC AGGCAGCGCT GAGGATTTGC
    GATGGAGACA TGGGGGCATC ACTGGAACGC CTGCTCACCC AGTGCTTTTC AGAGACATTT
    GGAGAGAGGA TGAAGATATC TGAGGCAGTT GCACACATGA GCTTGGAAGA GTGTGTGGAA
    CAGCGACAGG AAGAGGCATT TGCTCTCAAG TCAATCTGTG GAGAAAAATT TATAGAAAGA
    ATTCAAAACA GAGTCTGGAC CATTGGATTA GAACTGGAGT ATTTGACAAG TAAATTCTGC
    AAATTCAAGC AAAAGGAAAG TACCAAAAAT ATACAGGAGG CTTCACTTGA AGTCTGCAAA
    TTTTACCTCA AAGGAAATTG TAAATTTGGA TCAAAGTGCA AATTTAAACA TGAAGTGCCC
    CCAAATCAAA TTGTCGGAAG AGTCGAAAGA AATGTAGATG ATTCTCATCT AAATGCTAAT
    GAAGATGCAT CCTTTTTATA TGAACTTGAA ATTCGATTTT CTAAAGACCA CAAATACCCC
    TACCAAGCTC CCCTTGTGGC ATTTTATTCC ACCAATGAGA ATCTACCTCT GGCTTGTCGT
    TTACATATTT CTGAGTTCCT TTATGGCAAA GCCTTGACAT TTGCAGAAAC TTCAGAACCT
    GTTGTATATT CTTTGATAAC CCTCTTAGAG GAAGAATCAG AAATAACCAA GTTACTAACA
    AATACCCATC ACAAGTACAG TGTCCCTCCT GTGAACTTTC TGCCAGTATC ACCTGGAACC
    AGACTAAATA ATCCTGTCTG TCGTAAACCA GTGATTCCAA GTAATTCGTT TTTTTCGAAT
    CAAATTCCAG AAGTTGAAAA AGAATCAGAG CCTGAGGAGG AGACAGATGA GGATGAAGGT
    CCAGCACCAG TTATGGTGGA GAATGAAAGC TATGTCAACC TTAAGAAAAA GATTTCCAAA
    AGATATGACT GGCAGGCAAA ATCAGTACAT GCTGAAAATG TCAAAATCTG CAAGCAGTTC
    CGAATAAAAC AGGCCTCCAG ACAGTTCCAG TCAGTTCTAC AAGAAAGACA GTCGCTCCCT
    GCTTGGGAAG AAAGAGAAAA CATTCTCAAA TTGTTGAGCA AGCACCAGGT GCTTGTTGTA
    AGTGGTATGA CTGGATGTGG GAAAACCACA CAGATTCCAC AGTTTATTCT GGATGATTCT
    CTGAATGGAC CCCCTGAGAA GGTGGCTAAC ATCATCTGTA CCCAACCCCG ACGAATCTCT
    GCCATCTCTG TTGCTGAGCG CGTTGCTAAA GAAAGGGCAG AAAGAGTGGG TCTGACCGTG
    GGATACCAGA TCCGGTTAGA AAGTGTCAAG TCCTCAGCCA CCAGGTTGTT ATACTGCACC
    ACGGGAGTGC TGCTAAGAAG GCTGGAGGGA GACACAGCTC TGCAGGGAGT CACCCATATC
    ATTGTTGATG AAGTTCACGA GAGGACAGAA GAAAGTGACT TCTTACTACT AGTTTTGAAA
    GACATTGTGT TGCAGAGACC AACTCTTCAA GTTATTCTGA TGAGTGCAAC CTTAAACGCT
    GAGCTCTTTT CAGAATATTT TAATTCCTGC CCAGTTATCA CTATACCAGG TCGTACATTT
    CCTGTTGATC AGTTTTTTCT GGAAGATGCA ATTGCTGTGA CACGGTATGT GTTGCAGGAT
    GGGAGCCCAT ACGCCCGCTC CATGAAACAG ATGTCAAAGG AGAAGCTCAA GGCCAGGCGC
    AGCAGAACCG CATTTGAGGA GGTGGAGGAG GACCTGCGTC TCTCCCTGCA CCTCCAGGAT
    CAGGATTCTG TCAAAGATGC GGTACCAGAT CAACAATTAG ATTTTAAACA GCTCCTTGCC
    CGCTATCAAG GAGTTAGCAA ATCAGTCATC AAAACAATGT CCGTTATGGA TTTTGAAAAG
    GTTAATCTTG AATTAATCGA GGCCTTGTTA GAGTGGATTG TGGACGGAAA GCACTCCTAC
    CCTCCAGGTG CTATACTTGT ATTTTTACCG GGACTAGCAG AAATCAAAAT GCTGTATGAA
    CAGCTCCAGT CTAATTCTCT TTTCAACAAT AGACGTAGTA ATCGATGTGT TATTCACCCA
    CTTCATTCGT CTTTATCCAG TGAAGAGCAG CAGGCTGTGT TTGTAAAACC TCCTATGGGT
    GTAACTAAGA TTATAATTTC CACCAATATT GCTGAGACAT CCATAACCAT CGATGATGTT
    GTCTATGTCA TTGATTCTGG GAAAATGAAA GAAAAGAGAT ATGATGCCAG CAAAGGGATG
    GAAAGTTTAG AGGATACTTT TGTCTCTCAA GCCAATGCCC TGCAAAGAAA AGGTCGAGCA
    GGTCGTGTAG CGTCTGGGGT CTGCTTCCAT TTATTCACCA GCCATCACTT TAATCACCAA
    CTTCTAAAGC AGCAGCTACC AGAAATACAA AGAGTGCCAT TGGAACAGCT CTGTCTGAGA
    ATTAAAATTT TAGAGATGTT TAGCACTCAC AATCTCCAGT CTGTGTTCGC TCGGCTCATT
    GAACCCCCAC ACGCTGATTC TCTCCGTGCC TCAAAAATAC GATTACGAGA CTTGGGAGCA
    TTAACTCCAG ACGAAACACT GACCCCTCTA GGGTATCACT TGGCCTCTTT GCCTGTAGAT
    GTGAGAATCG GCAAACTAAT GCTCTTTGGA TCTATCTTCC GCTGTTTGGA TCCTGCTCTC
    ACCATTGCTG CCAGTTTAGC TTTTAAGTCT CCTTTTGTAT CTCCCTGGGA TAAAAAAGAG
    GAAGCTAACC AGAAAAAGCT AGAATTTGCA TTTGCAAACA GTGATTATCT GGCCCTTCTA
    CGAGCATATA AGGGATGGCA GCTAAGTATG AAAGAAGGTA TGCGTGCAAG TTATAATTAC
    TGCAGACAAA ACTTCTTGTC AGGAAGAGTT CTTCAGGAAA TGGCAAGCCT GAAACGACAG
    TTCACTGAAC TGTTATCGGA CATAGGCTTT GTTAAGGAGG GACTCCGAGC AAGGGAAATT
    GAGAAAAGAG CCCAAGGAGG AGGAGATGGT ATATTGGATG CCACAGGAGA AGAGGCAAAC
    TCAAATGCTG AGAACCCCAA GTTGATATCA GCAATGCTGT GTGCTGCTTT GTATCCAAAT
    GTAGTGCAGG TGAAAAGCCC AGAGGGGAAA TTTCAGAAGA CCAGTACCGG AGCTGTCAGA
    ATGCAGCCAA AATCAGATGA GTTGAAGTTT GTCACCAAGA ACGATGGATA CGTACACATT
    CACCCTTCAT CGGTGAACTA TCAGGTCAGA CACTTTGACA GTCCCTACCT GGTATACCAC
    GAGAAGATCA AAACTAGTCG CGTGTTCATC CGAGACTGCA GCATGGTGTC TGTGTACCCA
    CTGGTCTTAT TTGGAGGAGG CCAAGTGAAT GTGCAGCTCC AAAAGGGAGA GTTTGTTGTT
    TCCTTGGATG ATGGTTGGAT CCGCTTTGCA GCTGCTTCCC ATCAGGTGGC AGAATTAGTA
    AAGGAACTTC GTTGTGAACT TGACCAGCTC CTCCAGGATA AGATAAAAAA CCCAAGCATA
    GATCTGTGCA CCTGTCCTCG AGGGTCACGG ATCATCAGCA TGATTGTGAA ACTTGTCACC
    ACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91874
    Clone ID Related Accession (Same CDS sequence) XM_015473454.1
    Accession Version XM_015473454.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative ATP-dependent RNA helicase DHX57 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGGAAAAC CAAGAAAGGA TGCGGGGTGG CCTTCTCCTT ACTCCTTTCT GCCTGCTCCT 
    GACACAGGTG CTTCCCTGGC GTGGCCCACG GTAACAGACA GACCTCTCCT TTTTAGGAGA
    GCTGTGAAAA TGAGTTCTTC AGTAAGAAGG AAAGGCAAGC CAGGGAAAGG AGGTGGAAAA
    GGGTCTTCCA GAGGAGGAAG AGGAGGCAGG AGCCATGCTA GTAAATCTCA TGGGAGTGGC
    AGCAGTGGCG GTGGTGTTGG TGGCAACAGA AAGGCATCAA GTAGAATCTG GGATGATGGC
    GATGACTTTT GTATCTTCAG TGAATCAAGG CATTCATCCA GACCTAGTAA CAGTAGCGTG
    AGAAAAGGGG ATGGACGCCC CAAATGGAAA CCCGAAGCCA AAGTGCCCCT GCAGACTCTA
    CATATGACTT CTGAGAATCA GGAAAAAGTG AAAGCTCTTC TCCGAGACTT GCAGGAACAA
    GATGCGGATG CTGGATCTGA AAGAGGTGTT TCTGGGGAAG AAGAAGATGA TGAGCCTGAT
    TATGACGACG AGCAGTACTG GTCACCTGGA CGAGAAGCTT CCCTTGTTCC TGACTCTGAT
    CCTTTGGAAT GCGCTGGCTC AGGCCTGGTG GAGCCTCATA ACCCAGAATT TACAGTCTCA
    GCATTTGCAG TGCACAGACT TTCCAGGTAT GGTTTCCACA CTGAACACTG TCAGGCAGCG
    CTGAGGATTT GCGATGGAGA CATGGGGGCA TCACTGGAAC GCCTGCTCAC CCAGTGCTTT
    TCAGAGACAT TTGGAGAGAG GATGAAGATA TCTGAGGCAG TTGCACACAT GAGCTTGGAA
    GAGTGTGTGG AACAGCGACA GGAAGAGGCA TTTGCTCTCA AGTCAATCTG TGGAGAAAAA
    TTTATAGAAA GAATTCAAAA CAGAGTCTGG ACCATTGGAT TAGAACTGGA GTATTTGACA
    AGTAAATTCT GCAAATTCAA GCAAAAGGAA AGTACCAAAA ATATACAGGA GGCTTCACTT
    GAAGTCTGCA AATTTTACCT CAAAGGAAAT TGTAAATTTG GATCAAAGTG CAAATTTAAA
    CATGAAGTGC CCCCAAATCA AATTGTCGGA AGAGTCGAAA GAAATGTAGA TGATTCTCAT
    CTAAATGCTA ATGAAGATGC ATCCTTTTTA TATGAACTTG AAATTCGATT TTCTAAAGAC
    CACAAATACC CCTACCAAGC TCCCCTTGTG GCATTTTATT CCACCAATGA GAATCTACCT
    CTGGCTTGTC GTTTACATAT TTCTGAGTTC CTTTATGGCA AAGCCTTGAC ATTTGCAGAA
    ACTTCAGAAC CTGTTGTATA TTCTTTGATA ACCCTCTTAG AGGAAGAATC AGAAATAACC
    AAGTTACTAA CAAATACCCA TCACAAGTAC AGTGTCCCTC CTGTGAACTT TCTGCCAGTA
    TCACCTGGAA CCAGACTAAA TAATCCTGTC TGTCGTAAAC CAGTGATTCC AAGTAATTCG
    TTTTTTTCGA ATCAAATTCC AGAAGTTGAA AAAGAATCAG AGCCTGAGGA GGAGACAGAT
    GAGGATGAAG GTCCAGCACC AGTTATGGTG GAGAATGAAA GCTATGTCAA CCTTAAGAAA
    AAGATTTCCA AAAGATATGA CTGGCAGGCA AAATCAGTAC ATGCTGAAAA TGTCAAAATC
    TGCAAGCAGT TCCGAATAAA ACAGGCCTCC AGACAGTTCC AGTCAGTTCT ACAAGAAAGA
    CAGTCGCTCC CTGCTTGGGA AGAAAGAGAA AACATTCTCA AATTGTTGAG CAAGCACCAG
    GTGCTTGTTG TAAGTGGTAT GACTGGATGT GGGAAAACCA CACAGATTCC ACAGTTTATT
    CTGGATGATT CTCTGAATGG ACCCCCTGAG AAGGTGGCTA ACATCATCTG TACCCAACCC
    CGACGAATCT CTGCCATCTC TGTTGCTGAG CGCGTTGCTA AAGAAAGGGC AGAAAGAGTG
    GGTCTGACTG TGGGATACCA GATCCGGTTA GAAAGTGTCA AGTCCTCAGC CACCAGGTTG
    TTATACTGCA CCACGGGAGT GCTGCTAAGA AGGCTGGAGG GAGACACAGC TCTGCAGGGA
    GTCACCCATA TCATTGTTGA TGAAGTTCAC GAGAGGACAG AAGAAAGTGA CTTCTTACTA
    CTAGTTTTGA AAGACATTGT GTTGCAGAGA CCAACTCTTC AAGTTATTCT GATGAGTGCA
    ACCTTAAACG CTGAGCTCTT TTCAGAATAT TTTAATTCCT GCCCAGTTAT CACTATACCA
    GGTCGTACAT TTCCTGTTGA TCAGTTTTTT CTGGAAGATG CAATTGCTGT GACACGGTAT
    GTGTTGCAGG ATGGGAGCCC ATACGCCCGC TCCATGAAAC AGATGTCAAA GGAGAAGCTC
    AAGGCCAGGC GCAGCAGAAC CGCATTTGAG GAGGTGGAGG AGGACCTGCG TCTCTCCCTG
    CACCTCCAGG ATCAGGATTC TGTCAAAGAT GCGGTACCAG ATCAACAATT AGATTTTAAA
    CAGCTCCTTG CCCGCTATCA AGGAGTTAGC AAATCAGTCA TCAAAACAAT GTCCGTTATG
    GATTTTGAAA AGGTTAATCT TGAATTAATC GAGGCCTTGT TAGAGTGGAT TGTGGACGGA
    AAGCACTCCT ACCCTCCAGG TGCTATACTT GTATTTTTAC CGGGACTAGC AGAAATCAAA
    ATGCTGTATG AACAGCTCCA GTCTAATTCT CTTTTCAACA ATAGACGTAG TAATCGATGT
    GTTATTCACC CACTTCATTC GTCTTTATCC AGTGAAGAGC AGCAGGCTGT GTTTGTAAAA
    CCTCCTATGG GTGTAACTAA GATTATAATT TCCACCAATA TTGCTGAGAC ATCCATAACC
    ATCGATGATG TTGTCTATGT CATTGATTCT GGGAAAATGA AAGAAAAGAG ATATGATGCC
    AGCAAAGGGA TGGAAAGTTT AGAGGATACT TTTGTCTCTC AAGCCAATGC CCTGCAAAGA
    AAAGGTCGAG CAGGTCGTGT AGCGTCTGGG GTCTGCTTCC ATTTATTCAC CAGCCATCAC
    TTTAATCACC AACTTCTAAA GCAGCAGCTA CCAGAAATAC AAAGAGTGCC ATTGGAACAG
    CTCTGTCTGA GAATTAAAAT TTTAGAGATG TTTAGCACTC ACAATCTCCA GTCTGTGTTC
    GCTCGGCTCA TTGAACCCCC ACACGCTGAT TCTCTCCGTG CCTCAAAAAT ACGATTACGA
    GACTTGGGAG CATTAACTCC AGACGAAACA CTGACCCCTC TAGGGTATCA CTTGGCCTCT
    TTGCCTGTAG ATGTGAGAAT CGGCAAACTA ATGCTCTTTG GATCTATCTT CCGCTGTTTG
    GATCCTGCTC TCACCATTGC TGCCAGTTTA GCTTTTAAGT CTCCTTTTGT ATCTCCCTGG
    GATAAAAAAG AGGAAGCTAA CCAGAAAAAG CTAGAATTTG CATTTGCAAA CAGTGATTAT
    CTGGCCCTTC TACGAGCATA TAAGGGATGG CAGCTAAGTA TGAAAGAAGG TATGCGTGCA
    AGTTATAATT ACTGCAGACA AAACTTCTTG TCAGGAAGAG TTCTTCAGGA AATGGCAAGC
    CTGAAACGAC AGTTCACTGA ACTGTTATCG GACATAGGCT TTGTTAAGGA GGGACTCCGA
    GCAAGGGAAA TTGAGAAAAG AGCCCAAGGA GGAGGAGATG GTATATTGGA TGCCACAGGA
    GAAGAGGCAA ACTCAAATGC TGAGAACCCC AAGTTGATAT CAGCAATGCT GTGTGCTGCT
    CTGTATCCAA ATGTAGTGCA GGTGAAAAGC CCAGAGGGGA AATTTCAGAA GACCAGTACC
    GGAGCTGTCA GAATGCAGCC AAAATCAGAT GAGTTGAAGT TTGTCACCAA GAACGATGGA
    TACGTACACA TTCACCCTTC ATCGGTGAAC TATCAGGTCA GACACTTTGA CAGTCCCTAC
    CTGGTATACC ACGAGAAGAT CAAAACTAGT CGCGTGTTCA TCCGAGACTG CAGCATGGTG
    TCTGTGTACC CACTGGTCTT ATTTGGAGGA GGCCAAGTGA ATGTGCAGCT CCAAAAGGGA
    GAGTTTGTTG TTTCCTTGGA TGATGGTTGG ATCCGCTTTG CAGCTGCTTC CCATCAGGTG
    GCAGAATTAG TAAAGGAACT TCGTTGTGAA CTTGACCAGC TCCTCCAGGA TAAGATAAAA
    AACCCAAGCA TAGATCTGTG CACCTGTCCT CGAGGGTCAC GGATCATCAG CATGATTGTG
    AAACTTGTCA CCACACAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328940.1
    CDS16..4296
    Translation

    Target ORF information:

    RefSeq Version XM_015473454.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DEAH-box helicase 57 (DHX57), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473454.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGGAAAAC CAAGAAAGGA TGCGGGGTGG CCTTCTCCTT ACTCCTTTCT GCCTGCTCCT 
    GACACAGGTG CTTCCCTGGC GTGGCCCACG GTAACAGACA GACCTCTCCT TTTTAGGAGA
    GCTGTGAAAA TGAGTTCTTC AGTAAGAAGG AAAGGCAAGC CAGGGAAAGG AGGTGGAAAA
    GGGTCTTCCA GAGGAGGAAG AGGAGGCAGG AGCCATGCTA GTAAATCTCA TGGGAGTGGC
    AGCAGTGGCG GTGGTGTTGG TGGCAACAGA AAGGCATCAA GTAGAATCTG GGATGATGGC
    GATGACTTTT GTATCTTCAG TGAATCAAGG CATTCATCCA GACCTAGTAA CAGTAGCGTG
    AGAAAAGGGG ATGGACGCCC CAAATGGAAA CCCGAAGCCA AAGTGCCCCT GCAGACTCTA
    CATATGACTT CTGAGAATCA GGAAAAAGTG AAAGCTCTTC TCCGAGACTT GCAGGAACAA
    GATGCGGATG CTGGATCTGA AAGAGGTGTT TCTGGGGAAG AAGAAGATGA TGAGCCTGAT
    TATGACGACG AGCAGTACTG GTCACCTGGA CGAGAAGCTT CCCTTGTTCC TGACTCTGAT
    CCTTTGGAAT GCGCTGGCTC AGGCCTGGTG GAGCCTCATA ACCCAGAATT TACAGTCTCA
    GCATTTGCAG TGCACAGACT TTCCAGGTAT GGTTTCCACA CTGAACACTG TCAGGCAGCG
    CTGAGGATTT GCGATGGAGA CATGGGGGCA TCACTGGAAC GCCTGCTCAC CCAGTGCTTT
    TCAGAGACAT TTGGAGAGAG GATGAAGATA TCTGAGGCAG TTGCACACAT GAGCTTGGAA
    GAGTGTGTGG AACAGCGACA GGAAGAGGCA TTTGCTCTCA AGTCAATCTG TGGAGAAAAA
    TTTATAGAAA GAATTCAAAA CAGAGTCTGG ACCATTGGAT TAGAACTGGA GTATTTGACA
    AGTAAATTCT GCAAATTCAA GCAAAAGGAA AGTACCAAAA ATATACAGGA GGCTTCACTT
    GAAGTCTGCA AATTTTACCT CAAAGGAAAT TGTAAATTTG GATCAAAGTG CAAATTTAAA
    CATGAAGTGC CCCCAAATCA AATTGTCGGA AGAGTCGAAA GAAATGTAGA TGATTCTCAT
    CTAAATGCTA ATGAAGATGC ATCCTTTTTA TATGAACTTG AAATTCGATT TTCTAAAGAC
    CACAAATACC CCTACCAAGC TCCCCTTGTG GCATTTTATT CCACCAATGA GAATCTACCT
    CTGGCTTGTC GTTTACATAT TTCTGAGTTC CTTTATGGCA AAGCCTTGAC ATTTGCAGAA
    ACTTCAGAAC CTGTTGTATA TTCTTTGATA ACCCTCTTAG AGGAAGAATC AGAAATAACC
    AAGTTACTAA CAAATACCCA TCACAAGTAC AGTGTCCCTC CTGTGAACTT TCTGCCAGTA
    TCACCTGGAA CCAGACTAAA TAATCCTGTC TGTCGTAAAC CAGTGATTCC AAGTAATTCG
    TTTTTTTCGA ATCAAATTCC AGAAGTTGAA AAAGAATCAG AGCCTGAGGA GGAGACAGAT
    GAGGATGAAG GTCCAGCACC AGTTATGGTG GAGAATGAAA GCTATGTCAA CCTTAAGAAA
    AAGATTTCCA AAAGATATGA CTGGCAGGCA AAATCAGTAC ATGCTGAAAA TGTCAAAATC
    TGCAAGCAGT TCCGAATAAA ACAGGCCTCC AGACAGTTCC AGTCAGTTCT ACAAGAAAGA
    CAGTCGCTCC CTGCTTGGGA AGAAAGAGAA AACATTCTCA AATTGTTGAG CAAGCACCAG
    GTGCTTGTTG TAAGTGGTAT GACTGGATGT GGGAAAACCA CACAGATTCC ACAGTTTATT
    CTGGATGATT CTCTGAATGG ACCCCCTGAG AAGGTGGCTA ACATCATCTG TACCCAACCC
    CGACGAATCT CTGCCATCTC TGTTGCTGAG CGCGTTGCTA AAGAAAGGGC AGAAAGAGTG
    GGTCTGACTG TGGGATACCA GATCCGGTTA GAAAGTGTCA AGTCCTCAGC CACCAGGTTG
    TTATACTGCA CCACGGGAGT GCTGCTAAGA AGGCTGGAGG GAGACACAGC TCTGCAGGGA
    GTCACCCATA TCATTGTTGA TGAAGTTCAC GAGAGGACAG AAGAAAGTGA CTTCTTACTA
    CTAGTTTTGA AAGACATTGT GTTGCAGAGA CCAACTCTTC AAGTTATTCT GATGAGTGCA
    ACCTTAAACG CTGAGCTCTT TTCAGAATAT TTTAATTCCT GCCCAGTTAT CACTATACCA
    GGTCGTACAT TTCCTGTTGA TCAGTTTTTT CTGGAAGATG CAATTGCTGT GACACGGTAT
    GTGTTGCAGG ATGGGAGCCC ATACGCCCGC TCCATGAAAC AGATGTCAAA GGAGAAGCTC
    AAGGCCAGGC GCAGCAGAAC CGCATTTGAG GAGGTGGAGG AGGACCTGCG TCTCTCCCTG
    CACCTCCAGG ATCAGGATTC TGTCAAAGAT GCGGTACCAG ATCAACAATT AGATTTTAAA
    CAGCTCCTTG CCCGCTATCA AGGAGTTAGC AAATCAGTCA TCAAAACAAT GTCCGTTATG
    GATTTTGAAA AGGTTAATCT TGAATTAATC GAGGCCTTGT TAGAGTGGAT TGTGGACGGA
    AAGCACTCCT ACCCTCCAGG TGCTATACTT GTATTTTTAC CGGGACTAGC AGAAATCAAA
    ATGCTGTATG AACAGCTCCA GTCTAATTCT CTTTTCAACA ATAGACGTAG TAATCGATGT
    GTTATTCACC CACTTCATTC GTCTTTATCC AGTGAAGAGC AGCAGGCTGT GTTTGTAAAA
    CCTCCTATGG GTGTAACTAA GATTATAATT TCCACCAATA TTGCTGAGAC ATCCATAACC
    ATCGATGATG TTGTCTATGT CATTGATTCT GGGAAAATGA AAGAAAAGAG ATATGATGCC
    AGCAAAGGGA TGGAAAGTTT AGAGGATACT TTTGTCTCTC AAGCCAATGC CCTGCAAAGA
    AAAGGTCGAG CAGGTCGTGT AGCGTCTGGG GTCTGCTTCC ATTTATTCAC CAGCCATCAC
    TTTAATCACC AACTTCTAAA GCAGCAGCTA CCAGAAATAC AAAGAGTGCC ATTGGAACAG
    CTCTGTCTGA GAATTAAAAT TTTAGAGATG TTTAGCACTC ACAATCTCCA GTCTGTGTTC
    GCTCGGCTCA TTGAACCCCC ACACGCTGAT TCTCTCCGTG CCTCAAAAAT ACGATTACGA
    GACTTGGGAG CATTAACTCC AGACGAAACA CTGACCCCTC TAGGGTATCA CTTGGCCTCT
    TTGCCTGTAG ATGTGAGAAT CGGCAAACTA ATGCTCTTTG GATCTATCTT CCGCTGTTTG
    GATCCTGCTC TCACCATTGC TGCCAGTTTA GCTTTTAAGT CTCCTTTTGT ATCTCCCTGG
    GATAAAAAAG AGGAAGCTAA CCAGAAAAAG CTAGAATTTG CATTTGCAAA CAGTGATTAT
    CTGGCCCTTC TACGAGCATA TAAGGGATGG CAGCTAAGTA TGAAAGAAGG TATGCGTGCA
    AGTTATAATT ACTGCAGACA AAACTTCTTG TCAGGAAGAG TTCTTCAGGA AATGGCAAGC
    CTGAAACGAC AGTTCACTGA ACTGTTATCG GACATAGGCT TTGTTAAGGA GGGACTCCGA
    GCAAGGGAAA TTGAGAAAAG AGCCCAAGGA GGAGGAGATG GTATATTGGA TGCCACAGGA
    GAAGAGGCAA ACTCAAATGC TGAGAACCCC AAGTTGATAT CAGCAATGCT GTGTGCTGCT
    CTGTATCCAA ATGTAGTGCA GGTGAAAAGC CCAGAGGGGA AATTTCAGAA GACCAGTACC
    GGAGCTGTCA GAATGCAGCC AAAATCAGAT GAGTTGAAGT TTGTCACCAA GAACGATGGA
    TACGTACACA TTCACCCTTC ATCGGTGAAC TATCAGGTCA GACACTTTGA CAGTCCCTAC
    CTGGTATACC ACGAGAAGAT CAAAACTAGT CGCGTGTTCA TCCGAGACTG CAGCATGGTG
    TCTGTGTACC CACTGGTCTT ATTTGGAGGA GGCCAAGTGA ATGTGCAGCT CCAAAAGGGA
    GAGTTTGTTG TTTCCTTGGA TGATGGTTGG ATCCGCTTTG CAGCTGCTTC CCATCAGGTG
    GCAGAATTAG TAAAGGAACT TCGTTGTGAA CTTGACCAGC TCCTCCAGGA TAAGATAAAA
    AACCCAAGCA TAGATCTGTG CACCTGTCCT CGAGGGTCAC GGATCATCAG CATGATTGTG
    AAACTTGTCA CCACACAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23962
    Clone ID Related Accession (Same CDS sequence) XM_005212667.3 , NM_001206051.2 , XM_005212666.3
    Accession Version NM_001206051.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product putative ATP-dependent RNA helicase DHX57
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02030601.1 and DAAA02030599.1. On Jun 30, 2017 this sequence version replaced NM_001206051.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAAGAGGTGT TTCTGGGGAA GAAGAAGATG ATGAGCCTGA TTATGACGAC
    GAGCAGTACT GGTCACCTGG ACGAGAAGCT TCCCTTGTTC CTGACTCTGA TCCTTTGGAA
    TGCGCTGGCT CAGGCCTGGT GGAGCCTCAT AACCCAGAAT TTACAGTCTC AGCATTTGCA
    GTGCACAGAC TTTCCAGGTA TGGTTTCCAC ACTGAACACT GTCAGGCAGC GCTGAGGATT
    TGCGATGGAG ACATGGGGGC ATCACTGGAA CGCCTGCTCA CCCAGTGCTT TTCAGAGACA
    TTTGGAGAGA GGATGAAGAT ATCTGAGGCA GTTGCACACA TGAGCTTGGA AGAGTGTGTG
    GAACAGCGAC AGGAAGAGGC ATTTGCTCTC AAGTCAATCT GTGGAGAAAA ATTTATAGAA
    AGAATTCAAA ACAGAGTCTG GACCATTGGA TTAGAACTGG AGTATTTGAC AAGTAAATTC
    TGCAAATTCA AGCAAAAGGA AAGTACCAAA AATATACAGG AGGCTTCACT TGAAGTCTGC
    AAATTTTACC TCAAAGGAAA TTGTAAATTT GGATCAAAGT GCAAATTTAA ACATGAAGTG
    CCCCCAAATC AAATTGTCGG AAGAGTCGAA AGAAATGTAG ATGATTCTCA TCTAAATGCT
    AATGAAGATG CATCCTTTTT ATATGAACTT GAAATTCGAT TTTCTAAAGA CCACAAATAC
    CCCTACCAAG CTCCCCTTGT GGCATTTTAT TCCACCAATG AGAATCTACC TCTGGCTTGT
    CGTTTACATA TTTCTGAGTT CCTTTATGGC AAAGCCTTGA CATTTGCAGA AACTTCAGAA
    CCTGTTGTAT ATTCTTTGAT AACCCTCTTA GAGGAAGAAT CAGAAATAAC CAAGTTACTA
    ACAAATACCC ATCACAAGTA CAGTGTCCCT CCTGTGAACT TTCTGCCAGT ATCACCTGGA
    ACCAGACTAA ATAATCCTGT CTGTCGTAAA CCAGTGATTC CAAGTAATTC GTTTTTTTCG
    AATCAAATTC CAGAAGTTGA AAAAGAATCA GAGCCTGAGG AGGAGACAGA TGAGGATGAA
    GGTCCAGCAC CAGTTATGGT GGAGAATGAA AGCTATGTCA ACCTTAAGAA AAAGATTTCC
    AAAAGATATG ACTGGCAGGC AAAATCAGTA CATGCTGAAA ATGTCAAAAT CTGCAAGCAG
    TTCCGAATAA AACAGGCCTC CAGACAGTTC CAGTCAGTTC TACAAGAAAG ACAGTCGCTC
    CCTGCTTGGG AAGAAAGAGA AAACATTCTC AAATTGTTGA GCAAGCACCA GGTGCTTGTT
    GTAAGTGGTA TGACTGGATG TGGGAAAACC ACACAGATTC CACAGTTTAT TCTGGATGAT
    TCTCTGAATG GACCCCCTGA GAAGGTGGCT AACATCATCT GTACCCAACC CCGACGAATC
    TCTGCCATCT CTGTTGCTGA GCGCGTTGCT AAAGAAAGGG CAGAAAGAGT GGGTCTGACT
    GTGGGATACC AGATCCGGTT AGAAAGTGTC AAGTCCTCAG CCACCAGGTT GTTATACTGC
    ACCACGGGAG TGCTGCTAAG AAGGCTGGAG GGAGACACAG CTCTGCAGGG AGTCACCCAT
    ATCATTGTTG ATGAAGTTCA CGAGAGGACA GAAGAAAGTG ACTTCTTACT ACTAGTTTTG
    AAAGACATTG TGTTGCAGAG ACCAACTCTT CAAGTTATTC TGATGAGTGC AACCTTAAAC
    GCTGAGCTCT TTTCAGAATA TTTTAATTCC TGCCCAGTTA TCACTATACC AGGTCGTACA
    TTTCCTGTTG ATCAGTTTTT TCTGGAAGAT GCAATTGCTG TGACACGGTA TGTGTTGCAG
    GATGGGAGCC CATACGCCCG CTCCATGAAA CAGATGTCAA AGGAGAAGCT CAAGGCCAGG
    CGCAGCAGAA CCGCATTTGA GGAGGTGGAG GAGGACCTGC GTCTCTCCCT GCACCTCCAG
    GATCAGGATT CTGTCAAAGA TGCGGTACCA GATCAACAAT TAGATTTTAA ACAGCTCCTT
    GCCCGCTATC AAGGAGTTAG CAAATCAGTC ATCAAAACAA TGTCCGTTAT GGATTTTGAA
    AAGGTTAATC TTGAATTAAT CGAGGCCTTG TTAGAGTGGA TTGTGGACGG AAAGCACTCC
    TACCCTCCAG GTGCTATACT TGTATTTTTA CCGGGACTAG CAGAAATCAA AATGCTGTAT
    GAACAGCTCC AGTCTAATTC TCTTTTCAAC AATAGACGTA GTAATCGATG TGTTATTCAC
    CCACTTCATT CGTCTTTATC CAGTGAAGAG CAGCAGGCTG TGTTTGTAAA ACCTCCTATG
    GGTGTAACTA AGATTATAAT TTCCACCAAT ATTGCTGAGA CATCCATAAC CATCGATGAT
    GTTGTCTATG TCATTGATTC TGGGAAAATG AAAGAAAAGA GATATGATGC CAGCAAAGGG
    ATGGAAAGTT TAGAGGATAC TTTTGTCTCT CAAGCCAATG CCCTGCAAAG AAAAGGTCGA
    GCAGGTCGTG TAGCGTCTGG GGTCTGCTTC CATTTATTCA CCAGCCATCA CTTTAATCAC
    CAACTTCTAA AGCAGCAGCT ACCAGAAATA CAAAGAGTGC CATTGGAACA GCTCTGTCTG
    AGAATTAAAA TTTTAGAGAT GTTTAGCACT CACAATCTCC AGTCTGTGTT CGCTCGGCTC
    ATTGAACCCC CACACGCTGA TTCTCTCCGT GCCTCAAAAA TACGATTACG AGACTTGGGA
    GCATTAACTC CAGACGAAAC ACTGACCCCT CTAGGGTATC ACTTGGCCTC TTTGCCTGTA
    GATGTGAGAA TCGGCAAACT AATGCTCTTT GGATCTATCT TCCGCTGTTT GGATCCTGCT
    CTCACCATTG CTGCCAGTTT AGCTTTTAAG TCTCCTTTTG TATCTCCCTG GGATAAAAAA
    GAGGAAGCTA ACCAGAAAAA GCTAGAATTT GCATTTGCAA ACAGTGATTA TCTGGCCCTT
    CTACGAGCAT ATAAGGGATG GCAGCTAAGT ATGAAAGAAG GTATGCGTGC AAGTTATAAT
    TACTGCAGAC AAAACTTCTT GTCAGGAAGA GTTCTTCAGG AAATGGCAAG CCTGAAACGA
    CAGTTCACTG AACTGTTATC GGACATAGGC TTTGTTAAGG AGGGACTCCG AGCAAGGGAA
    ATTGAGAAAA GAGCCCAAGG AGGAGGAGAT GGTATATTGG ATGCCACAGG AGAAGAGGCA
    AACTCAAATG CTGAGAACCC CAAGTTGATA TCAGCAATGC TGTGTGCTGC TCTGTATCCA
    AATGTAGTGC AGGTGAAAAG CCCAGAGGGG AAATTTCAGA AGACCAGTAC CGGAGCTGTC
    AGAATGCAGC CAAAATCAGA TGAGTTGAAG TTTGTCACCA AGAACGATGG ATACGTACAC
    ATTCACCCTT CATCGGTGAA CTATCAGGTC AGACACTTTG ACAGTCCCTA CCTGGTATAC
    CACGAGAAGA TCAAAACTAG TCGCGTGTTC ATCCGAGACT GCAGCATGGT GTCTGTGTAC
    CCACTGGTCT TATTTGGAGG AGGCCAAGTG AATGTGCAGC TCCAAAAGGG AGAGTTTGTT
    GTTTCCTTGG ATGATGGTTG GATCCGCTTT GCAGCTGCTT CCCATCAGGT GGCAGAATTA
    GTAAAGGAAC TTCGTTGTGA ACTTGACCAG CTCCTCCAGG ATAAGATAAA AAACCCAAGC
    ATAGATCTGT GCACCTGTCC TCGAGGGTCA CGGATCATCA GCATGATTGT GAAACTTGTC
    ACCACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192980.1
    CDS1..4152
    Translation

    Target ORF information:

    RefSeq Version NM_001206051.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DExH-box helicase 57 (DHX57), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206051.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAAGAGGTGT TTCTGGGGAA GAAGAAGATG ATGAGCCTGA TTATGACGAC
    GAGCAGTACT GGTCACCTGG ACGAGAAGCT TCCCTTGTTC CTGACTCTGA TCCTTTGGAA
    TGCGCTGGCT CAGGCCTGGT GGAGCCTCAT AACCCAGAAT TTACAGTCTC AGCATTTGCA
    GTGCACAGAC TTTCCAGGTA TGGTTTCCAC ACTGAACACT GTCAGGCAGC GCTGAGGATT
    TGCGATGGAG ACATGGGGGC ATCACTGGAA CGCCTGCTCA CCCAGTGCTT TTCAGAGACA
    TTTGGAGAGA GGATGAAGAT ATCTGAGGCA GTTGCACACA TGAGCTTGGA AGAGTGTGTG
    GAACAGCGAC AGGAAGAGGC ATTTGCTCTC AAGTCAATCT GTGGAGAAAA ATTTATAGAA
    AGAATTCAAA ACAGAGTCTG GACCATTGGA TTAGAACTGG AGTATTTGAC AAGTAAATTC
    TGCAAATTCA AGCAAAAGGA AAGTACCAAA AATATACAGG AGGCTTCACT TGAAGTCTGC
    AAATTTTACC TCAAAGGAAA TTGTAAATTT GGATCAAAGT GCAAATTTAA ACATGAAGTG
    CCCCCAAATC AAATTGTCGG AAGAGTCGAA AGAAATGTAG ATGATTCTCA TCTAAATGCT
    AATGAAGATG CATCCTTTTT ATATGAACTT GAAATTCGAT TTTCTAAAGA CCACAAATAC
    CCCTACCAAG CTCCCCTTGT GGCATTTTAT TCCACCAATG AGAATCTACC TCTGGCTTGT
    CGTTTACATA TTTCTGAGTT CCTTTATGGC AAAGCCTTGA CATTTGCAGA AACTTCAGAA
    CCTGTTGTAT ATTCTTTGAT AACCCTCTTA GAGGAAGAAT CAGAAATAAC CAAGTTACTA
    ACAAATACCC ATCACAAGTA CAGTGTCCCT CCTGTGAACT TTCTGCCAGT ATCACCTGGA
    ACCAGACTAA ATAATCCTGT CTGTCGTAAA CCAGTGATTC CAAGTAATTC GTTTTTTTCG
    AATCAAATTC CAGAAGTTGA AAAAGAATCA GAGCCTGAGG AGGAGACAGA TGAGGATGAA
    GGTCCAGCAC CAGTTATGGT GGAGAATGAA AGCTATGTCA ACCTTAAGAA AAAGATTTCC
    AAAAGATATG ACTGGCAGGC AAAATCAGTA CATGCTGAAA ATGTCAAAAT CTGCAAGCAG
    TTCCGAATAA AACAGGCCTC CAGACAGTTC CAGTCAGTTC TACAAGAAAG ACAGTCGCTC
    CCTGCTTGGG AAGAAAGAGA AAACATTCTC AAATTGTTGA GCAAGCACCA GGTGCTTGTT
    GTAAGTGGTA TGACTGGATG TGGGAAAACC ACACAGATTC CACAGTTTAT TCTGGATGAT
    TCTCTGAATG GACCCCCTGA GAAGGTGGCT AACATCATCT GTACCCAACC CCGACGAATC
    TCTGCCATCT CTGTTGCTGA GCGCGTTGCT AAAGAAAGGG CAGAAAGAGT GGGTCTGACT
    GTGGGATACC AGATCCGGTT AGAAAGTGTC AAGTCCTCAG CCACCAGGTT GTTATACTGC
    ACCACGGGAG TGCTGCTAAG AAGGCTGGAG GGAGACACAG CTCTGCAGGG AGTCACCCAT
    ATCATTGTTG ATGAAGTTCA CGAGAGGACA GAAGAAAGTG ACTTCTTACT ACTAGTTTTG
    AAAGACATTG TGTTGCAGAG ACCAACTCTT CAAGTTATTC TGATGAGTGC AACCTTAAAC
    GCTGAGCTCT TTTCAGAATA TTTTAATTCC TGCCCAGTTA TCACTATACC AGGTCGTACA
    TTTCCTGTTG ATCAGTTTTT TCTGGAAGAT GCAATTGCTG TGACACGGTA TGTGTTGCAG
    GATGGGAGCC CATACGCCCG CTCCATGAAA CAGATGTCAA AGGAGAAGCT CAAGGCCAGG
    CGCAGCAGAA CCGCATTTGA GGAGGTGGAG GAGGACCTGC GTCTCTCCCT GCACCTCCAG
    GATCAGGATT CTGTCAAAGA TGCGGTACCA GATCAACAAT TAGATTTTAA ACAGCTCCTT
    GCCCGCTATC AAGGAGTTAG CAAATCAGTC ATCAAAACAA TGTCCGTTAT GGATTTTGAA
    AAGGTTAATC TTGAATTAAT CGAGGCCTTG TTAGAGTGGA TTGTGGACGG AAAGCACTCC
    TACCCTCCAG GTGCTATACT TGTATTTTTA CCGGGACTAG CAGAAATCAA AATGCTGTAT
    GAACAGCTCC AGTCTAATTC TCTTTTCAAC AATAGACGTA GTAATCGATG TGTTATTCAC
    CCACTTCATT CGTCTTTATC CAGTGAAGAG CAGCAGGCTG TGTTTGTAAA ACCTCCTATG
    GGTGTAACTA AGATTATAAT TTCCACCAAT ATTGCTGAGA CATCCATAAC CATCGATGAT
    GTTGTCTATG TCATTGATTC TGGGAAAATG AAAGAAAAGA GATATGATGC CAGCAAAGGG
    ATGGAAAGTT TAGAGGATAC TTTTGTCTCT CAAGCCAATG CCCTGCAAAG AAAAGGTCGA
    GCAGGTCGTG TAGCGTCTGG GGTCTGCTTC CATTTATTCA CCAGCCATCA CTTTAATCAC
    CAACTTCTAA AGCAGCAGCT ACCAGAAATA CAAAGAGTGC CATTGGAACA GCTCTGTCTG
    AGAATTAAAA TTTTAGAGAT GTTTAGCACT CACAATCTCC AGTCTGTGTT CGCTCGGCTC
    ATTGAACCCC CACACGCTGA TTCTCTCCGT GCCTCAAAAA TACGATTACG AGACTTGGGA
    GCATTAACTC CAGACGAAAC ACTGACCCCT CTAGGGTATC ACTTGGCCTC TTTGCCTGTA
    GATGTGAGAA TCGGCAAACT AATGCTCTTT GGATCTATCT TCCGCTGTTT GGATCCTGCT
    CTCACCATTG CTGCCAGTTT AGCTTTTAAG TCTCCTTTTG TATCTCCCTG GGATAAAAAA
    GAGGAAGCTA ACCAGAAAAA GCTAGAATTT GCATTTGCAA ACAGTGATTA TCTGGCCCTT
    CTACGAGCAT ATAAGGGATG GCAGCTAAGT ATGAAAGAAG GTATGCGTGC AAGTTATAAT
    TACTGCAGAC AAAACTTCTT GTCAGGAAGA GTTCTTCAGG AAATGGCAAG CCTGAAACGA
    CAGTTCACTG AACTGTTATC GGACATAGGC TTTGTTAAGG AGGGACTCCG AGCAAGGGAA
    ATTGAGAAAA GAGCCCAAGG AGGAGGAGAT GGTATATTGG ATGCCACAGG AGAAGAGGCA
    AACTCAAATG CTGAGAACCC CAAGTTGATA TCAGCAATGC TGTGTGCTGC TCTGTATCCA
    AATGTAGTGC AGGTGAAAAG CCCAGAGGGG AAATTTCAGA AGACCAGTAC CGGAGCTGTC
    AGAATGCAGC CAAAATCAGA TGAGTTGAAG TTTGTCACCA AGAACGATGG ATACGTACAC
    ATTCACCCTT CATCGGTGAA CTATCAGGTC AGACACTTTG ACAGTCCCTA CCTGGTATAC
    CACGAGAAGA TCAAAACTAG TCGCGTGTTC ATCCGAGACT GCAGCATGGT GTCTGTGTAC
    CCACTGGTCT TATTTGGAGG AGGCCAAGTG AATGTGCAGC TCCAAAAGGG AGAGTTTGTT
    GTTTCCTTGG ATGATGGTTG GATCCGCTTT GCAGCTGCTT CCCATCAGGT GGCAGAATTA
    GTAAAGGAAC TTCGTTGTGA ACTTGACCAG CTCCTCCAGG ATAAGATAAA AAACCCAAGC
    ATAGATCTGT GCACCTGTCC TCGAGGGTCA CGGATCATCA GCATGATTGT GAAACTTGTC
    ACCACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36966
    Clone ID Related Accession (Same CDS sequence) NM_001206051.1 , XM_005212667.4 , NM_001206051.3 , XM_005212666.4
    Accession Version NM_001206051.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product putative ATP-dependent RNA helicase DHX57
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC02005441.1, AAFC03090970.1, AAFC02126970.1 and AAFC03090971.1. On Apr 20, 2011 this sequence version replaced XM_614021.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAAGAGGTGT TTCTGGGGAA GAAGAAGATG ATGAGCCTGA TTATGACGAC
    GAGCAGTACT GGTCACCTGG ACGAGAAGCT TCCCTTGTTC CTGACTCTGA TCCTTTGGAA
    TGCGCTGGCT CAGGCCTGGT GGAGCCTCAT AACCCAGAAT TTACAGTCTC AGCATTTGCA
    GTGCACAGAC TTTCCAGGTA TGGTTTCCAC ACTGAACACT GTCAGGCAGC GCTGAGGATT
    TGCGATGGAG ACATGGGGGC ATCACTGGAA CGCCTGCTCA CCCAGTGCTT TTCAGAGACA
    TTTGGAGAGA GGATGAAGAT ATCTGAGGCA GTTGCACACA TGAGCTTGGA AGAGTGTGTG
    GAACAGCGAC AGGAAGAGGC ATTTGCTCTC AAGTCAATCT GTGGAGAAAA ATTTATAGAA
    AGAATTCAAA ACAGAGTCTG GACCATTGGA TTAGAACTGG AGTATTTGAC AAGTAAATTC
    TGCAAATTCA AGCAAAAGGA AAGTACCAAA AATATACAGG AGGCTTCACT TGAAGTCTGC
    AAATTTTACC TCAAAGGAAA TTGTAAATTT GGATCAAAGT GCAAATTTAA ACATGAAGTG
    CCCCCAAATC AAATTGTCGG AAGAGTCGAA AGAAATGTAG ATGATTCTCA TCTAAATGCT
    AATGAAGATG CATCCTTTTT ATATGAACTT GAAATTCGAT TTTCTAAAGA CCACAAATAC
    CCCTACCAAG CTCCCCTTGT GGCATTTTAT TCCACCAATG AGAATCTACC TCTGGCTTGT
    CGTTTACATA TTTCTGAGTT CCTTTATGGC AAAGCCTTGA CATTTGCAGA AACTTCAGAA
    CCTGTTGTAT ATTCTTTGAT AACCCTCTTA GAGGAAGAAT CAGAAATAAC CAAGTTACTA
    ACAAATACCC ATCACAAGTA CAGTGTCCCT CCTGTGAACT TTCTGCCAGT ATCACCTGGA
    ACCAGACTAA ATAATCCTGT CTGTCGTAAA CCAGTGATTC CAAGTAATTC GTTTTTTTCG
    AATCAAATTC CAGAAGTTGA AAAAGAATCA GAGCCTGAGG AGGAGACAGA TGAGGATGAA
    GGTCCAGCAC CAGTTATGGT GGAGAATGAA AGCTATGTCA ACCTTAAGAA AAAGATTTCC
    AAAAGATATG ACTGGCAGGC AAAATCAGTA CATGCTGAAA ATGTCAAAAT CTGCAAGCAG
    TTCCGAATAA AACAGGCCTC CAGACAGTTC CAGTCAGTTC TACAAGAAAG ACAGTCGCTC
    CCTGCTTGGG AAGAAAGAGA AAACATTCTC AAATTGTTGA GCAAGCACCA GGTGCTTGTT
    GTAAGTGGTA TGACTGGATG TGGGAAAACC ACACAGATTC CACAGTTTAT TCTGGATGAT
    TCTCTGAATG GACCCCCTGA GAAGGTGGCT AACATCATCT GTACCCAACC CCGACGAATC
    TCTGCCATCT CTGTTGCTGA GCGCGTTGCT AAAGAAAGGG CAGAAAGAGT GGGTCTGACC
    GTGGGATACC AGATCCGGTT AGAAAGTGTC AAGTCCTCAG CCACCAGGTT GTTATACTGC
    ACCACGGGAG TGCTGCTAAG AAGGCTGGAG GGAGACACAG CTCTGCAGGG AGTCACCCAT
    ATCATTGTTG ATGAAGTTCA CGAGAGGACA GAAGAAAGTG ACTTCTTACT ACTAGTTTTG
    AAAGACATTG TGTTGCAGAG ACCAACTCTT CAAGTTATTC TGATGAGTGC AACCTTAAAC
    GCTGAGCTCT TTTCAGAATA TTTTAATTCC TGCCCAGTTA TCACTATACC AGGTCGTACA
    TTTCCTGTTG ATCAGTTTTT TCTGGAAGAT GCAATTGCTG TGACACGGTA TGTGTTGCAG
    GATGGGAGCC CATACGCCCG CTCCATGAAA CAGATGTCAA AGGAGAAGCT CAAGGCCAGG
    CGCAGCAGAA CCGCATTTGA GGAGGTGGAG GAGGACCTGC GTCTCTCCCT GCACCTCCAG
    GATCAGGATT CTGTCAAAGA TGCGGTACCA GATCAACAAT TAGATTTTAA ACAGCTCCTT
    GCCCGCTATC AAGGAGTTAG CAAATCAGTC ATCAAAACAA TGTCCGTTAT GGATTTTGAA
    AAGGTTAATC TTGAATTAAT CGAGGCCTTG TTAGAGTGGA TTGTGGACGG AAAGCACTCC
    TACCCTCCAG GTGCTATACT TGTATTTTTA CCGGGACTAG CAGAAATCAA AATGCTGTAT
    GAACAGCTCC AGTCTAATTC TCTTTTCAAC AATAGACGTA GTAATCGATG TGTTATTCAC
    CCACTTCATT CGTCTTTATC CAGTGAAGAG CAGCAGGCTG TGTTTGTAAA ACCTCCTATG
    GGTGTAACTA AGATTATAAT TTCCACCAAT ATTGCTGAGA CATCCATAAC CATCGATGAT
    GTTGTCTATG TCATTGATTC TGGGAAAATG AAAGAAAAGA GATATGATGC CAGCAAAGGG
    ATGGAAAGTT TAGAGGATAC TTTTGTCTCT CAAGCCAATG CCCTGCAAAG AAAAGGTCGA
    GCAGGTCGTG TAGCGTCTGG GGTCTGCTTC CATTTATTCA CCAGCCATCA CTTTAATCAC
    CAACTTCTAA AGCAGCAGCT ACCAGAAATA CAAAGAGTGC CATTGGAACA GCTCTGTCTG
    AGAATTAAAA TTTTAGAGAT GTTTAGCACT CACAATCTCC AGTCTGTGTT CGCTCGGCTC
    ATTGAACCCC CACACGCTGA TTCTCTCCGT GCCTCAAAAA TACGATTACG AGACTTGGGA
    GCATTAACTC CAGACGAAAC ACTGACCCCT CTAGGGTATC ACTTGGCCTC TTTGCCTGTA
    GATGTGAGAA TCGGCAAACT AATGCTCTTT GGATCTATCT TCCGCTGTTT GGATCCTGCT
    CTCACCATTG CTGCCAGTTT AGCTTTTAAG TCTCCTTTTG TATCTCCCTG GGATAAAAAA
    GAGGAAGCTA ACCAGAAAAA GCTAGAATTT GCATTTGCAA ACAGTGATTA TCTGGCCCTT
    CTACGAGCAT ATAAGGGATG GCAGCTAAGT ATGAAAGAAG GTATGCGTGC AAGTTATAAT
    TACTGCAGAC AAAACTTCTT GTCAGGAAGA GTTCTTCAGG AAATGGCAAG CCTGAAACGA
    CAGTTCACTG AACTGTTATC GGACATAGGC TTTGTTAAGG AGGGACTCCG AGCAAGGGAA
    ATTGAGAAAA GAGCCCAAGG AGGAGGAGAT GGTATATTGG ATGCCACAGG AGAAGAGGCA
    AACTCAAATG CTGAGAACCC CAAGTTGATA TCAGCAATGC TGTGTGCTGC TTTGTATCCA
    AATGTAGTGC AGGTGAAAAG CCCAGAGGGG AAATTTCAGA AGACCAGTAC CGGAGCTGTC
    AGAATGCAGC CAAAATCAGA TGAGTTGAAG TTTGTCACCA AGAACGATGG ATACGTACAC
    ATTCACCCTT CATCGGTGAA CTATCAGGTC AGACACTTTG ACAGTCCCTA CCTGGTATAC
    CACGAGAAGA TCAAAACTAG TCGCGTGTTC ATCCGAGACT GCAGCATGGT GTCTGTGTAC
    CCACTGGTCT TATTTGGAGG AGGCCAAGTG AATGTGCAGC TCCAAAAGGG AGAGTTTGTT
    GTTTCCTTGG ATGATGGTTG GATCCGCTTT GCAGCTGCTT CCCATCAGGT GGCAGAATTA
    GTAAAGGAAC TTCGTTGTGA ACTTGACCAG CTCCTCCAGG ATAAGATAAA AAACCCAAGC
    ATAGATCTGT GCACCTGTCC TCGAGGGTCA CGGATCATCA GCATGATTGT GAAACTTGTC
    ACCACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192980.1
    CDS1..4152
    Translation

    Target ORF information:

    RefSeq Version NM_001206051.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57 (DHX57), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206051.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAAGAGGTGT TTCTGGGGAA GAAGAAGATG ATGAGCCTGA TTATGACGAC
    GAGCAGTACT GGTCACCTGG ACGAGAAGCT TCCCTTGTTC CTGACTCTGA TCCTTTGGAA
    TGCGCTGGCT CAGGCCTGGT GGAGCCTCAT AACCCAGAAT TTACAGTCTC AGCATTTGCA
    GTGCACAGAC TTTCCAGGTA TGGTTTCCAC ACTGAACACT GTCAGGCAGC GCTGAGGATT
    TGCGATGGAG ACATGGGGGC ATCACTGGAA CGCCTGCTCA CCCAGTGCTT TTCAGAGACA
    TTTGGAGAGA GGATGAAGAT ATCTGAGGCA GTTGCACACA TGAGCTTGGA AGAGTGTGTG
    GAACAGCGAC AGGAAGAGGC ATTTGCTCTC AAGTCAATCT GTGGAGAAAA ATTTATAGAA
    AGAATTCAAA ACAGAGTCTG GACCATTGGA TTAGAACTGG AGTATTTGAC AAGTAAATTC
    TGCAAATTCA AGCAAAAGGA AAGTACCAAA AATATACAGG AGGCTTCACT TGAAGTCTGC
    AAATTTTACC TCAAAGGAAA TTGTAAATTT GGATCAAAGT GCAAATTTAA ACATGAAGTG
    CCCCCAAATC AAATTGTCGG AAGAGTCGAA AGAAATGTAG ATGATTCTCA TCTAAATGCT
    AATGAAGATG CATCCTTTTT ATATGAACTT GAAATTCGAT TTTCTAAAGA CCACAAATAC
    CCCTACCAAG CTCCCCTTGT GGCATTTTAT TCCACCAATG AGAATCTACC TCTGGCTTGT
    CGTTTACATA TTTCTGAGTT CCTTTATGGC AAAGCCTTGA CATTTGCAGA AACTTCAGAA
    CCTGTTGTAT ATTCTTTGAT AACCCTCTTA GAGGAAGAAT CAGAAATAAC CAAGTTACTA
    ACAAATACCC ATCACAAGTA CAGTGTCCCT CCTGTGAACT TTCTGCCAGT ATCACCTGGA
    ACCAGACTAA ATAATCCTGT CTGTCGTAAA CCAGTGATTC CAAGTAATTC GTTTTTTTCG
    AATCAAATTC CAGAAGTTGA AAAAGAATCA GAGCCTGAGG AGGAGACAGA TGAGGATGAA
    GGTCCAGCAC CAGTTATGGT GGAGAATGAA AGCTATGTCA ACCTTAAGAA AAAGATTTCC
    AAAAGATATG ACTGGCAGGC AAAATCAGTA CATGCTGAAA ATGTCAAAAT CTGCAAGCAG
    TTCCGAATAA AACAGGCCTC CAGACAGTTC CAGTCAGTTC TACAAGAAAG ACAGTCGCTC
    CCTGCTTGGG AAGAAAGAGA AAACATTCTC AAATTGTTGA GCAAGCACCA GGTGCTTGTT
    GTAAGTGGTA TGACTGGATG TGGGAAAACC ACACAGATTC CACAGTTTAT TCTGGATGAT
    TCTCTGAATG GACCCCCTGA GAAGGTGGCT AACATCATCT GTACCCAACC CCGACGAATC
    TCTGCCATCT CTGTTGCTGA GCGCGTTGCT AAAGAAAGGG CAGAAAGAGT GGGTCTGACC
    GTGGGATACC AGATCCGGTT AGAAAGTGTC AAGTCCTCAG CCACCAGGTT GTTATACTGC
    ACCACGGGAG TGCTGCTAAG AAGGCTGGAG GGAGACACAG CTCTGCAGGG AGTCACCCAT
    ATCATTGTTG ATGAAGTTCA CGAGAGGACA GAAGAAAGTG ACTTCTTACT ACTAGTTTTG
    AAAGACATTG TGTTGCAGAG ACCAACTCTT CAAGTTATTC TGATGAGTGC AACCTTAAAC
    GCTGAGCTCT TTTCAGAATA TTTTAATTCC TGCCCAGTTA TCACTATACC AGGTCGTACA
    TTTCCTGTTG ATCAGTTTTT TCTGGAAGAT GCAATTGCTG TGACACGGTA TGTGTTGCAG
    GATGGGAGCC CATACGCCCG CTCCATGAAA CAGATGTCAA AGGAGAAGCT CAAGGCCAGG
    CGCAGCAGAA CCGCATTTGA GGAGGTGGAG GAGGACCTGC GTCTCTCCCT GCACCTCCAG
    GATCAGGATT CTGTCAAAGA TGCGGTACCA GATCAACAAT TAGATTTTAA ACAGCTCCTT
    GCCCGCTATC AAGGAGTTAG CAAATCAGTC ATCAAAACAA TGTCCGTTAT GGATTTTGAA
    AAGGTTAATC TTGAATTAAT CGAGGCCTTG TTAGAGTGGA TTGTGGACGG AAAGCACTCC
    TACCCTCCAG GTGCTATACT TGTATTTTTA CCGGGACTAG CAGAAATCAA AATGCTGTAT
    GAACAGCTCC AGTCTAATTC TCTTTTCAAC AATAGACGTA GTAATCGATG TGTTATTCAC
    CCACTTCATT CGTCTTTATC CAGTGAAGAG CAGCAGGCTG TGTTTGTAAA ACCTCCTATG
    GGTGTAACTA AGATTATAAT TTCCACCAAT ATTGCTGAGA CATCCATAAC CATCGATGAT
    GTTGTCTATG TCATTGATTC TGGGAAAATG AAAGAAAAGA GATATGATGC CAGCAAAGGG
    ATGGAAAGTT TAGAGGATAC TTTTGTCTCT CAAGCCAATG CCCTGCAAAG AAAAGGTCGA
    GCAGGTCGTG TAGCGTCTGG GGTCTGCTTC CATTTATTCA CCAGCCATCA CTTTAATCAC
    CAACTTCTAA AGCAGCAGCT ACCAGAAATA CAAAGAGTGC CATTGGAACA GCTCTGTCTG
    AGAATTAAAA TTTTAGAGAT GTTTAGCACT CACAATCTCC AGTCTGTGTT CGCTCGGCTC
    ATTGAACCCC CACACGCTGA TTCTCTCCGT GCCTCAAAAA TACGATTACG AGACTTGGGA
    GCATTAACTC CAGACGAAAC ACTGACCCCT CTAGGGTATC ACTTGGCCTC TTTGCCTGTA
    GATGTGAGAA TCGGCAAACT AATGCTCTTT GGATCTATCT TCCGCTGTTT GGATCCTGCT
    CTCACCATTG CTGCCAGTTT AGCTTTTAAG TCTCCTTTTG TATCTCCCTG GGATAAAAAA
    GAGGAAGCTA ACCAGAAAAA GCTAGAATTT GCATTTGCAA ACAGTGATTA TCTGGCCCTT
    CTACGAGCAT ATAAGGGATG GCAGCTAAGT ATGAAAGAAG GTATGCGTGC AAGTTATAAT
    TACTGCAGAC AAAACTTCTT GTCAGGAAGA GTTCTTCAGG AAATGGCAAG CCTGAAACGA
    CAGTTCACTG AACTGTTATC GGACATAGGC TTTGTTAAGG AGGGACTCCG AGCAAGGGAA
    ATTGAGAAAA GAGCCCAAGG AGGAGGAGAT GGTATATTGG ATGCCACAGG AGAAGAGGCA
    AACTCAAATG CTGAGAACCC CAAGTTGATA TCAGCAATGC TGTGTGCTGC TTTGTATCCA
    AATGTAGTGC AGGTGAAAAG CCCAGAGGGG AAATTTCAGA AGACCAGTAC CGGAGCTGTC
    AGAATGCAGC CAAAATCAGA TGAGTTGAAG TTTGTCACCA AGAACGATGG ATACGTACAC
    ATTCACCCTT CATCGGTGAA CTATCAGGTC AGACACTTTG ACAGTCCCTA CCTGGTATAC
    CACGAGAAGA TCAAAACTAG TCGCGTGTTC ATCCGAGACT GCAGCATGGT GTCTGTGTAC
    CCACTGGTCT TATTTGGAGG AGGCCAAGTG AATGTGCAGC TCCAAAAGGG AGAGTTTGTT
    GTTTCCTTGG ATGATGGTTG GATCCGCTTT GCAGCTGCTT CCCATCAGGT GGCAGAATTA
    GTAAAGGAAC TTCGTTGTGA ACTTGACCAG CTCCTCCAGG ATAAGATAAA AAACCCAAGC
    ATAGATCTGT GCACCTGTCC TCGAGGGTCA CGGATCATCA GCATGATTGT GAAACTTGTC
    ACCACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36966
    Clone ID Related Accession (Same CDS sequence) NM_001206051.1 , XM_005212667.4 , NM_001206051.3 , XM_005212666.4
    Accession Version NM_001206051.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product putative ATP-dependent RNA helicase DHX57
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000011.1. On May 29, 2018 this sequence version replaced NM_001206051.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5721696.6.1, SRR5571257.53424.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAAGAGGTGT TTCTGGGGAA GAAGAAGATG ATGAGCCTGA TTATGACGAC
    GAGCAGTACT GGTCACCTGG ACGAGAAGCT TCCCTTGTTC CTGACTCTGA TCCTTTGGAA
    TGCGCTGGCT CAGGCCTGGT GGAGCCTCAT AACCCAGAAT TTACAGTCTC AGCATTTGCA
    GTGCACAGAC TTTCCAGGTA TGGTTTCCAC ACTGAACACT GTCAGGCAGC GCTGAGGATT
    TGCGATGGAG ACATGGGGGC ATCACTGGAA CGCCTGCTCA CCCAGTGCTT TTCAGAGACA
    TTTGGAGAGA GGATGAAGAT ATCTGAGGCA GTTGCACACA TGAGCTTGGA AGAGTGTGTG
    GAACAGCGAC AGGAAGAGGC ATTTGCTCTC AAGTCAATCT GTGGAGAAAA ATTTATAGAA
    AGAATTCAAA ACAGAGTCTG GACCATTGGA TTAGAACTGG AGTATTTGAC AAGTAAATTC
    TGCAAATTCA AGCAAAAGGA AAGTACCAAA AATATACAGG AGGCTTCACT TGAAGTCTGC
    AAATTTTACC TCAAAGGAAA TTGTAAATTT GGATCAAAGT GCAAATTTAA ACATGAAGTG
    CCCCCAAATC AAATTGTCGG AAGAGTCGAA AGAAATGTAG ATGATTCTCA TCTAAATGCT
    AATGAAGATG CATCCTTTTT ATATGAACTT GAAATTCGAT TTTCTAAAGA CCACAAATAC
    CCCTACCAAG CTCCCCTTGT GGCATTTTAT TCCACCAATG AGAATCTACC TCTGGCTTGT
    CGTTTACATA TTTCTGAGTT CCTTTATGGC AAAGCCTTGA CATTTGCAGA AACTTCAGAA
    CCTGTTGTAT ATTCTTTGAT AACCCTCTTA GAGGAAGAAT CAGAAATAAC CAAGTTACTA
    ACAAATACCC ATCACAAGTA CAGTGTCCCT CCTGTGAACT TTCTGCCAGT ATCACCTGGA
    ACCAGACTAA ATAATCCTGT CTGTCGTAAA CCAGTGATTC CAAGTAATTC GTTTTTTTCG
    AATCAAATTC CAGAAGTTGA AAAAGAATCA GAGCCTGAGG AGGAGACAGA TGAGGATGAA
    GGTCCAGCAC CAGTTATGGT GGAGAATGAA AGCTATGTCA ACCTTAAGAA AAAGATTTCC
    AAAAGATATG ACTGGCAGGC AAAATCAGTA CATGCTGAAA ATGTCAAAAT CTGCAAGCAG
    TTCCGAATAA AACAGGCCTC CAGACAGTTC CAGTCAGTTC TACAAGAAAG ACAGTCGCTC
    CCTGCTTGGG AAGAAAGAGA AAACATTCTC AAATTGTTGA GCAAGCACCA GGTGCTTGTT
    GTAAGTGGTA TGACTGGATG TGGGAAAACC ACACAGATTC CACAGTTTAT TCTGGATGAT
    TCTCTGAATG GACCCCCTGA GAAGGTGGCT AACATCATCT GTACCCAACC CCGACGAATC
    TCTGCCATCT CTGTTGCTGA GCGCGTTGCT AAAGAAAGGG CAGAAAGAGT GGGTCTGACC
    GTGGGATACC AGATCCGGTT AGAAAGTGTC AAGTCCTCAG CCACCAGGTT GTTATACTGC
    ACCACGGGAG TGCTGCTAAG AAGGCTGGAG GGAGACACAG CTCTGCAGGG AGTCACCCAT
    ATCATTGTTG ATGAAGTTCA CGAGAGGACA GAAGAAAGTG ACTTCTTACT ACTAGTTTTG
    AAAGACATTG TGTTGCAGAG ACCAACTCTT CAAGTTATTC TGATGAGTGC AACCTTAAAC
    GCTGAGCTCT TTTCAGAATA TTTTAATTCC TGCCCAGTTA TCACTATACC AGGTCGTACA
    TTTCCTGTTG ATCAGTTTTT TCTGGAAGAT GCAATTGCTG TGACACGGTA TGTGTTGCAG
    GATGGGAGCC CATACGCCCG CTCCATGAAA CAGATGTCAA AGGAGAAGCT CAAGGCCAGG
    CGCAGCAGAA CCGCATTTGA GGAGGTGGAG GAGGACCTGC GTCTCTCCCT GCACCTCCAG
    GATCAGGATT CTGTCAAAGA TGCGGTACCA GATCAACAAT TAGATTTTAA ACAGCTCCTT
    GCCCGCTATC AAGGAGTTAG CAAATCAGTC ATCAAAACAA TGTCCGTTAT GGATTTTGAA
    AAGGTTAATC TTGAATTAAT CGAGGCCTTG TTAGAGTGGA TTGTGGACGG AAAGCACTCC
    TACCCTCCAG GTGCTATACT TGTATTTTTA CCGGGACTAG CAGAAATCAA AATGCTGTAT
    GAACAGCTCC AGTCTAATTC TCTTTTCAAC AATAGACGTA GTAATCGATG TGTTATTCAC
    CCACTTCATT CGTCTTTATC CAGTGAAGAG CAGCAGGCTG TGTTTGTAAA ACCTCCTATG
    GGTGTAACTA AGATTATAAT TTCCACCAAT ATTGCTGAGA CATCCATAAC CATCGATGAT
    GTTGTCTATG TCATTGATTC TGGGAAAATG AAAGAAAAGA GATATGATGC CAGCAAAGGG
    ATGGAAAGTT TAGAGGATAC TTTTGTCTCT CAAGCCAATG CCCTGCAAAG AAAAGGTCGA
    GCAGGTCGTG TAGCGTCTGG GGTCTGCTTC CATTTATTCA CCAGCCATCA CTTTAATCAC
    CAACTTCTAA AGCAGCAGCT ACCAGAAATA CAAAGAGTGC CATTGGAACA GCTCTGTCTG
    AGAATTAAAA TTTTAGAGAT GTTTAGCACT CACAATCTCC AGTCTGTGTT CGCTCGGCTC
    ATTGAACCCC CACACGCTGA TTCTCTCCGT GCCTCAAAAA TACGATTACG AGACTTGGGA
    GCATTAACTC CAGACGAAAC ACTGACCCCT CTAGGGTATC ACTTGGCCTC TTTGCCTGTA
    GATGTGAGAA TCGGCAAACT AATGCTCTTT GGATCTATCT TCCGCTGTTT GGATCCTGCT
    CTCACCATTG CTGCCAGTTT AGCTTTTAAG TCTCCTTTTG TATCTCCCTG GGATAAAAAA
    GAGGAAGCTA ACCAGAAAAA GCTAGAATTT GCATTTGCAA ACAGTGATTA TCTGGCCCTT
    CTACGAGCAT ATAAGGGATG GCAGCTAAGT ATGAAAGAAG GTATGCGTGC AAGTTATAAT
    TACTGCAGAC AAAACTTCTT GTCAGGAAGA GTTCTTCAGG AAATGGCAAG CCTGAAACGA
    CAGTTCACTG AACTGTTATC GGACATAGGC TTTGTTAAGG AGGGACTCCG AGCAAGGGAA
    ATTGAGAAAA GAGCCCAAGG AGGAGGAGAT GGTATATTGG ATGCCACAGG AGAAGAGGCA
    AACTCAAATG CTGAGAACCC CAAGTTGATA TCAGCAATGC TGTGTGCTGC TTTGTATCCA
    AATGTAGTGC AGGTGAAAAG CCCAGAGGGG AAATTTCAGA AGACCAGTAC CGGAGCTGTC
    AGAATGCAGC CAAAATCAGA TGAGTTGAAG TTTGTCACCA AGAACGATGG ATACGTACAC
    ATTCACCCTT CATCGGTGAA CTATCAGGTC AGACACTTTG ACAGTCCCTA CCTGGTATAC
    CACGAGAAGA TCAAAACTAG TCGCGTGTTC ATCCGAGACT GCAGCATGGT GTCTGTGTAC
    CCACTGGTCT TATTTGGAGG AGGCCAAGTG AATGTGCAGC TCCAAAAGGG AGAGTTTGTT
    GTTTCCTTGG ATGATGGTTG GATCCGCTTT GCAGCTGCTT CCCATCAGGT GGCAGAATTA
    GTAAAGGAAC TTCGTTGTGA ACTTGACCAG CTCCTCCAGG ATAAGATAAA AAACCCAAGC
    ATAGATCTGT GCACCTGTCC TCGAGGGTCA CGGATCATCA GCATGATTGT GAAACTTGTC
    ACCACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192980.1
    CDS1..4152
    Translation

    Target ORF information:

    RefSeq Version NM_001206051.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DExH-box helicase 57 (DHX57), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206051.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAAGAGGTGT TTCTGGGGAA GAAGAAGATG ATGAGCCTGA TTATGACGAC
    GAGCAGTACT GGTCACCTGG ACGAGAAGCT TCCCTTGTTC CTGACTCTGA TCCTTTGGAA
    TGCGCTGGCT CAGGCCTGGT GGAGCCTCAT AACCCAGAAT TTACAGTCTC AGCATTTGCA
    GTGCACAGAC TTTCCAGGTA TGGTTTCCAC ACTGAACACT GTCAGGCAGC GCTGAGGATT
    TGCGATGGAG ACATGGGGGC ATCACTGGAA CGCCTGCTCA CCCAGTGCTT TTCAGAGACA
    TTTGGAGAGA GGATGAAGAT ATCTGAGGCA GTTGCACACA TGAGCTTGGA AGAGTGTGTG
    GAACAGCGAC AGGAAGAGGC ATTTGCTCTC AAGTCAATCT GTGGAGAAAA ATTTATAGAA
    AGAATTCAAA ACAGAGTCTG GACCATTGGA TTAGAACTGG AGTATTTGAC AAGTAAATTC
    TGCAAATTCA AGCAAAAGGA AAGTACCAAA AATATACAGG AGGCTTCACT TGAAGTCTGC
    AAATTTTACC TCAAAGGAAA TTGTAAATTT GGATCAAAGT GCAAATTTAA ACATGAAGTG
    CCCCCAAATC AAATTGTCGG AAGAGTCGAA AGAAATGTAG ATGATTCTCA TCTAAATGCT
    AATGAAGATG CATCCTTTTT ATATGAACTT GAAATTCGAT TTTCTAAAGA CCACAAATAC
    CCCTACCAAG CTCCCCTTGT GGCATTTTAT TCCACCAATG AGAATCTACC TCTGGCTTGT
    CGTTTACATA TTTCTGAGTT CCTTTATGGC AAAGCCTTGA CATTTGCAGA AACTTCAGAA
    CCTGTTGTAT ATTCTTTGAT AACCCTCTTA GAGGAAGAAT CAGAAATAAC CAAGTTACTA
    ACAAATACCC ATCACAAGTA CAGTGTCCCT CCTGTGAACT TTCTGCCAGT ATCACCTGGA
    ACCAGACTAA ATAATCCTGT CTGTCGTAAA CCAGTGATTC CAAGTAATTC GTTTTTTTCG
    AATCAAATTC CAGAAGTTGA AAAAGAATCA GAGCCTGAGG AGGAGACAGA TGAGGATGAA
    GGTCCAGCAC CAGTTATGGT GGAGAATGAA AGCTATGTCA ACCTTAAGAA AAAGATTTCC
    AAAAGATATG ACTGGCAGGC AAAATCAGTA CATGCTGAAA ATGTCAAAAT CTGCAAGCAG
    TTCCGAATAA AACAGGCCTC CAGACAGTTC CAGTCAGTTC TACAAGAAAG ACAGTCGCTC
    CCTGCTTGGG AAGAAAGAGA AAACATTCTC AAATTGTTGA GCAAGCACCA GGTGCTTGTT
    GTAAGTGGTA TGACTGGATG TGGGAAAACC ACACAGATTC CACAGTTTAT TCTGGATGAT
    TCTCTGAATG GACCCCCTGA GAAGGTGGCT AACATCATCT GTACCCAACC CCGACGAATC
    TCTGCCATCT CTGTTGCTGA GCGCGTTGCT AAAGAAAGGG CAGAAAGAGT GGGTCTGACC
    GTGGGATACC AGATCCGGTT AGAAAGTGTC AAGTCCTCAG CCACCAGGTT GTTATACTGC
    ACCACGGGAG TGCTGCTAAG AAGGCTGGAG GGAGACACAG CTCTGCAGGG AGTCACCCAT
    ATCATTGTTG ATGAAGTTCA CGAGAGGACA GAAGAAAGTG ACTTCTTACT ACTAGTTTTG
    AAAGACATTG TGTTGCAGAG ACCAACTCTT CAAGTTATTC TGATGAGTGC AACCTTAAAC
    GCTGAGCTCT TTTCAGAATA TTTTAATTCC TGCCCAGTTA TCACTATACC AGGTCGTACA
    TTTCCTGTTG ATCAGTTTTT TCTGGAAGAT GCAATTGCTG TGACACGGTA TGTGTTGCAG
    GATGGGAGCC CATACGCCCG CTCCATGAAA CAGATGTCAA AGGAGAAGCT CAAGGCCAGG
    CGCAGCAGAA CCGCATTTGA GGAGGTGGAG GAGGACCTGC GTCTCTCCCT GCACCTCCAG
    GATCAGGATT CTGTCAAAGA TGCGGTACCA GATCAACAAT TAGATTTTAA ACAGCTCCTT
    GCCCGCTATC AAGGAGTTAG CAAATCAGTC ATCAAAACAA TGTCCGTTAT GGATTTTGAA
    AAGGTTAATC TTGAATTAAT CGAGGCCTTG TTAGAGTGGA TTGTGGACGG AAAGCACTCC
    TACCCTCCAG GTGCTATACT TGTATTTTTA CCGGGACTAG CAGAAATCAA AATGCTGTAT
    GAACAGCTCC AGTCTAATTC TCTTTTCAAC AATAGACGTA GTAATCGATG TGTTATTCAC
    CCACTTCATT CGTCTTTATC CAGTGAAGAG CAGCAGGCTG TGTTTGTAAA ACCTCCTATG
    GGTGTAACTA AGATTATAAT TTCCACCAAT ATTGCTGAGA CATCCATAAC CATCGATGAT
    GTTGTCTATG TCATTGATTC TGGGAAAATG AAAGAAAAGA GATATGATGC CAGCAAAGGG
    ATGGAAAGTT TAGAGGATAC TTTTGTCTCT CAAGCCAATG CCCTGCAAAG AAAAGGTCGA
    GCAGGTCGTG TAGCGTCTGG GGTCTGCTTC CATTTATTCA CCAGCCATCA CTTTAATCAC
    CAACTTCTAA AGCAGCAGCT ACCAGAAATA CAAAGAGTGC CATTGGAACA GCTCTGTCTG
    AGAATTAAAA TTTTAGAGAT GTTTAGCACT CACAATCTCC AGTCTGTGTT CGCTCGGCTC
    ATTGAACCCC CACACGCTGA TTCTCTCCGT GCCTCAAAAA TACGATTACG AGACTTGGGA
    GCATTAACTC CAGACGAAAC ACTGACCCCT CTAGGGTATC ACTTGGCCTC TTTGCCTGTA
    GATGTGAGAA TCGGCAAACT AATGCTCTTT GGATCTATCT TCCGCTGTTT GGATCCTGCT
    CTCACCATTG CTGCCAGTTT AGCTTTTAAG TCTCCTTTTG TATCTCCCTG GGATAAAAAA
    GAGGAAGCTA ACCAGAAAAA GCTAGAATTT GCATTTGCAA ACAGTGATTA TCTGGCCCTT
    CTACGAGCAT ATAAGGGATG GCAGCTAAGT ATGAAAGAAG GTATGCGTGC AAGTTATAAT
    TACTGCAGAC AAAACTTCTT GTCAGGAAGA GTTCTTCAGG AAATGGCAAG CCTGAAACGA
    CAGTTCACTG AACTGTTATC GGACATAGGC TTTGTTAAGG AGGGACTCCG AGCAAGGGAA
    ATTGAGAAAA GAGCCCAAGG AGGAGGAGAT GGTATATTGG ATGCCACAGG AGAAGAGGCA
    AACTCAAATG CTGAGAACCC CAAGTTGATA TCAGCAATGC TGTGTGCTGC TTTGTATCCA
    AATGTAGTGC AGGTGAAAAG CCCAGAGGGG AAATTTCAGA AGACCAGTAC CGGAGCTGTC
    AGAATGCAGC CAAAATCAGA TGAGTTGAAG TTTGTCACCA AGAACGATGG ATACGTACAC
    ATTCACCCTT CATCGGTGAA CTATCAGGTC AGACACTTTG ACAGTCCCTA CCTGGTATAC
    CACGAGAAGA TCAAAACTAG TCGCGTGTTC ATCCGAGACT GCAGCATGGT GTCTGTGTAC
    CCACTGGTCT TATTTGGAGG AGGCCAAGTG AATGTGCAGC TCCAAAAGGG AGAGTTTGTT
    GTTTCCTTGG ATGATGGTTG GATCCGCTTT GCAGCTGCTT CCCATCAGGT GGCAGAATTA
    GTAAAGGAAC TTCGTTGTGA ACTTGACCAG CTCCTCCAGG ATAAGATAAA AAACCCAAGC
    ATAGATCTGT GCACCTGTCC TCGAGGGTCA CGGATCATCA GCATGATTGT GAAACTTGTC
    ACCACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23962
    Clone ID Related Accession (Same CDS sequence) XM_005212667.3 , NM_001206051.2 , XM_005212666.3
    Accession Version XM_005212667.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative ATP-dependent RNA helicase DHX57 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212667.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAAGAGGTGT TTCTGGGGAA GAAGAAGATG ATGAGCCTGA TTATGACGAC
    GAGCAGTACT GGTCACCTGG ACGAGAAGCT TCCCTTGTTC CTGACTCTGA TCCTTTGGAA
    TGCGCTGGCT CAGGCCTGGT GGAGCCTCAT AACCCAGAAT TTACAGTCTC AGCATTTGCA
    GTGCACAGAC TTTCCAGGTA TGGTTTCCAC ACTGAACACT GTCAGGCAGC GCTGAGGATT
    TGCGATGGAG ACATGGGGGC ATCACTGGAA CGCCTGCTCA CCCAGTGCTT TTCAGAGACA
    TTTGGAGAGA GGATGAAGAT ATCTGAGGCA GTTGCACACA TGAGCTTGGA AGAGTGTGTG
    GAACAGCGAC AGGAAGAGGC ATTTGCTCTC AAGTCAATCT GTGGAGAAAA ATTTATAGAA
    AGAATTCAAA ACAGAGTCTG GACCATTGGA TTAGAACTGG AGTATTTGAC AAGTAAATTC
    TGCAAATTCA AGCAAAAGGA AAGTACCAAA AATATACAGG AGGCTTCACT TGAAGTCTGC
    AAATTTTACC TCAAAGGAAA TTGTAAATTT GGATCAAAGT GCAAATTTAA ACATGAAGTG
    CCCCCAAATC AAATTGTCGG AAGAGTCGAA AGAAATGTAG ATGATTCTCA TCTAAATGCT
    AATGAAGATG CATCCTTTTT ATATGAACTT GAAATTCGAT TTTCTAAAGA CCACAAATAC
    CCCTACCAAG CTCCCCTTGT GGCATTTTAT TCCACCAATG AGAATCTACC TCTGGCTTGT
    CGTTTACATA TTTCTGAGTT CCTTTATGGC AAAGCCTTGA CATTTGCAGA AACTTCAGAA
    CCTGTTGTAT ATTCTTTGAT AACCCTCTTA GAGGAAGAAT CAGAAATAAC CAAGTTACTA
    ACAAATACCC ATCACAAGTA CAGTGTCCCT CCTGTGAACT TTCTGCCAGT ATCACCTGGA
    ACCAGACTAA ATAATCCTGT CTGTCGTAAA CCAGTGATTC CAAGTAATTC GTTTTTTTCG
    AATCAAATTC CAGAAGTTGA AAAAGAATCA GAGCCTGAGG AGGAGACAGA TGAGGATGAA
    GGTCCAGCAC CAGTTATGGT GGAGAATGAA AGCTATGTCA ACCTTAAGAA AAAGATTTCC
    AAAAGATATG ACTGGCAGGC AAAATCAGTA CATGCTGAAA ATGTCAAAAT CTGCAAGCAG
    TTCCGAATAA AACAGGCCTC CAGACAGTTC CAGTCAGTTC TACAAGAAAG ACAGTCGCTC
    CCTGCTTGGG AAGAAAGAGA AAACATTCTC AAATTGTTGA GCAAGCACCA GGTGCTTGTT
    GTAAGTGGTA TGACTGGATG TGGGAAAACC ACACAGATTC CACAGTTTAT TCTGGATGAT
    TCTCTGAATG GACCCCCTGA GAAGGTGGCT AACATCATCT GTACCCAACC CCGACGAATC
    TCTGCCATCT CTGTTGCTGA GCGCGTTGCT AAAGAAAGGG CAGAAAGAGT GGGTCTGACT
    GTGGGATACC AGATCCGGTT AGAAAGTGTC AAGTCCTCAG CCACCAGGTT GTTATACTGC
    ACCACGGGAG TGCTGCTAAG AAGGCTGGAG GGAGACACAG CTCTGCAGGG AGTCACCCAT
    ATCATTGTTG ATGAAGTTCA CGAGAGGACA GAAGAAAGTG ACTTCTTACT ACTAGTTTTG
    AAAGACATTG TGTTGCAGAG ACCAACTCTT CAAGTTATTC TGATGAGTGC AACCTTAAAC
    GCTGAGCTCT TTTCAGAATA TTTTAATTCC TGCCCAGTTA TCACTATACC AGGTCGTACA
    TTTCCTGTTG ATCAGTTTTT TCTGGAAGAT GCAATTGCTG TGACACGGTA TGTGTTGCAG
    GATGGGAGCC CATACGCCCG CTCCATGAAA CAGATGTCAA AGGAGAAGCT CAAGGCCAGG
    CGCAGCAGAA CCGCATTTGA GGAGGTGGAG GAGGACCTGC GTCTCTCCCT GCACCTCCAG
    GATCAGGATT CTGTCAAAGA TGCGGTACCA GATCAACAAT TAGATTTTAA ACAGCTCCTT
    GCCCGCTATC AAGGAGTTAG CAAATCAGTC ATCAAAACAA TGTCCGTTAT GGATTTTGAA
    AAGGTTAATC TTGAATTAAT CGAGGCCTTG TTAGAGTGGA TTGTGGACGG AAAGCACTCC
    TACCCTCCAG GTGCTATACT TGTATTTTTA CCGGGACTAG CAGAAATCAA AATGCTGTAT
    GAACAGCTCC AGTCTAATTC TCTTTTCAAC AATAGACGTA GTAATCGATG TGTTATTCAC
    CCACTTCATT CGTCTTTATC CAGTGAAGAG CAGCAGGCTG TGTTTGTAAA ACCTCCTATG
    GGTGTAACTA AGATTATAAT TTCCACCAAT ATTGCTGAGA CATCCATAAC CATCGATGAT
    GTTGTCTATG TCATTGATTC TGGGAAAATG AAAGAAAAGA GATATGATGC CAGCAAAGGG
    ATGGAAAGTT TAGAGGATAC TTTTGTCTCT CAAGCCAATG CCCTGCAAAG AAAAGGTCGA
    GCAGGTCGTG TAGCGTCTGG GGTCTGCTTC CATTTATTCA CCAGCCATCA CTTTAATCAC
    CAACTTCTAA AGCAGCAGCT ACCAGAAATA CAAAGAGTGC CATTGGAACA GCTCTGTCTG
    AGAATTAAAA TTTTAGAGAT GTTTAGCACT CACAATCTCC AGTCTGTGTT CGCTCGGCTC
    ATTGAACCCC CACACGCTGA TTCTCTCCGT GCCTCAAAAA TACGATTACG AGACTTGGGA
    GCATTAACTC CAGACGAAAC ACTGACCCCT CTAGGGTATC ACTTGGCCTC TTTGCCTGTA
    GATGTGAGAA TCGGCAAACT AATGCTCTTT GGATCTATCT TCCGCTGTTT GGATCCTGCT
    CTCACCATTG CTGCCAGTTT AGCTTTTAAG TCTCCTTTTG TATCTCCCTG GGATAAAAAA
    GAGGAAGCTA ACCAGAAAAA GCTAGAATTT GCATTTGCAA ACAGTGATTA TCTGGCCCTT
    CTACGAGCAT ATAAGGGATG GCAGCTAAGT ATGAAAGAAG GTATGCGTGC AAGTTATAAT
    TACTGCAGAC AAAACTTCTT GTCAGGAAGA GTTCTTCAGG AAATGGCAAG CCTGAAACGA
    CAGTTCACTG AACTGTTATC GGACATAGGC TTTGTTAAGG AGGGACTCCG AGCAAGGGAA
    ATTGAGAAAA GAGCCCAAGG AGGAGGAGAT GGTATATTGG ATGCCACAGG AGAAGAGGCA
    AACTCAAATG CTGAGAACCC CAAGTTGATA TCAGCAATGC TGTGTGCTGC TCTGTATCCA
    AATGTAGTGC AGGTGAAAAG CCCAGAGGGG AAATTTCAGA AGACCAGTAC CGGAGCTGTC
    AGAATGCAGC CAAAATCAGA TGAGTTGAAG TTTGTCACCA AGAACGATGG ATACGTACAC
    ATTCACCCTT CATCGGTGAA CTATCAGGTC AGACACTTTG ACAGTCCCTA CCTGGTATAC
    CACGAGAAGA TCAAAACTAG TCGCGTGTTC ATCCGAGACT GCAGCATGGT GTCTGTGTAC
    CCACTGGTCT TATTTGGAGG AGGCCAAGTG AATGTGCAGC TCCAAAAGGG AGAGTTTGTT
    GTTTCCTTGG ATGATGGTTG GATCCGCTTT GCAGCTGCTT CCCATCAGGT GGCAGAATTA
    GTAAAGGAAC TTCGTTGTGA ACTTGACCAG CTCCTCCAGG ATAAGATAAA AAACCCAAGC
    ATAGATCTGT GCACCTGTCC TCGAGGGTCA CGGATCATCA GCATGATTGT GAAACTTGTC
    ACCACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212724.1
    CDS260..4411
    Translation

    Target ORF information:

    RefSeq Version XM_005212667.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DEAH-box helicase 57 (DHX57), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212667.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAAGAGGTGT TTCTGGGGAA GAAGAAGATG ATGAGCCTGA TTATGACGAC
    GAGCAGTACT GGTCACCTGG ACGAGAAGCT TCCCTTGTTC CTGACTCTGA TCCTTTGGAA
    TGCGCTGGCT CAGGCCTGGT GGAGCCTCAT AACCCAGAAT TTACAGTCTC AGCATTTGCA
    GTGCACAGAC TTTCCAGGTA TGGTTTCCAC ACTGAACACT GTCAGGCAGC GCTGAGGATT
    TGCGATGGAG ACATGGGGGC ATCACTGGAA CGCCTGCTCA CCCAGTGCTT TTCAGAGACA
    TTTGGAGAGA GGATGAAGAT ATCTGAGGCA GTTGCACACA TGAGCTTGGA AGAGTGTGTG
    GAACAGCGAC AGGAAGAGGC ATTTGCTCTC AAGTCAATCT GTGGAGAAAA ATTTATAGAA
    AGAATTCAAA ACAGAGTCTG GACCATTGGA TTAGAACTGG AGTATTTGAC AAGTAAATTC
    TGCAAATTCA AGCAAAAGGA AAGTACCAAA AATATACAGG AGGCTTCACT TGAAGTCTGC
    AAATTTTACC TCAAAGGAAA TTGTAAATTT GGATCAAAGT GCAAATTTAA ACATGAAGTG
    CCCCCAAATC AAATTGTCGG AAGAGTCGAA AGAAATGTAG ATGATTCTCA TCTAAATGCT
    AATGAAGATG CATCCTTTTT ATATGAACTT GAAATTCGAT TTTCTAAAGA CCACAAATAC
    CCCTACCAAG CTCCCCTTGT GGCATTTTAT TCCACCAATG AGAATCTACC TCTGGCTTGT
    CGTTTACATA TTTCTGAGTT CCTTTATGGC AAAGCCTTGA CATTTGCAGA AACTTCAGAA
    CCTGTTGTAT ATTCTTTGAT AACCCTCTTA GAGGAAGAAT CAGAAATAAC CAAGTTACTA
    ACAAATACCC ATCACAAGTA CAGTGTCCCT CCTGTGAACT TTCTGCCAGT ATCACCTGGA
    ACCAGACTAA ATAATCCTGT CTGTCGTAAA CCAGTGATTC CAAGTAATTC GTTTTTTTCG
    AATCAAATTC CAGAAGTTGA AAAAGAATCA GAGCCTGAGG AGGAGACAGA TGAGGATGAA
    GGTCCAGCAC CAGTTATGGT GGAGAATGAA AGCTATGTCA ACCTTAAGAA AAAGATTTCC
    AAAAGATATG ACTGGCAGGC AAAATCAGTA CATGCTGAAA ATGTCAAAAT CTGCAAGCAG
    TTCCGAATAA AACAGGCCTC CAGACAGTTC CAGTCAGTTC TACAAGAAAG ACAGTCGCTC
    CCTGCTTGGG AAGAAAGAGA AAACATTCTC AAATTGTTGA GCAAGCACCA GGTGCTTGTT
    GTAAGTGGTA TGACTGGATG TGGGAAAACC ACACAGATTC CACAGTTTAT TCTGGATGAT
    TCTCTGAATG GACCCCCTGA GAAGGTGGCT AACATCATCT GTACCCAACC CCGACGAATC
    TCTGCCATCT CTGTTGCTGA GCGCGTTGCT AAAGAAAGGG CAGAAAGAGT GGGTCTGACT
    GTGGGATACC AGATCCGGTT AGAAAGTGTC AAGTCCTCAG CCACCAGGTT GTTATACTGC
    ACCACGGGAG TGCTGCTAAG AAGGCTGGAG GGAGACACAG CTCTGCAGGG AGTCACCCAT
    ATCATTGTTG ATGAAGTTCA CGAGAGGACA GAAGAAAGTG ACTTCTTACT ACTAGTTTTG
    AAAGACATTG TGTTGCAGAG ACCAACTCTT CAAGTTATTC TGATGAGTGC AACCTTAAAC
    GCTGAGCTCT TTTCAGAATA TTTTAATTCC TGCCCAGTTA TCACTATACC AGGTCGTACA
    TTTCCTGTTG ATCAGTTTTT TCTGGAAGAT GCAATTGCTG TGACACGGTA TGTGTTGCAG
    GATGGGAGCC CATACGCCCG CTCCATGAAA CAGATGTCAA AGGAGAAGCT CAAGGCCAGG
    CGCAGCAGAA CCGCATTTGA GGAGGTGGAG GAGGACCTGC GTCTCTCCCT GCACCTCCAG
    GATCAGGATT CTGTCAAAGA TGCGGTACCA GATCAACAAT TAGATTTTAA ACAGCTCCTT
    GCCCGCTATC AAGGAGTTAG CAAATCAGTC ATCAAAACAA TGTCCGTTAT GGATTTTGAA
    AAGGTTAATC TTGAATTAAT CGAGGCCTTG TTAGAGTGGA TTGTGGACGG AAAGCACTCC
    TACCCTCCAG GTGCTATACT TGTATTTTTA CCGGGACTAG CAGAAATCAA AATGCTGTAT
    GAACAGCTCC AGTCTAATTC TCTTTTCAAC AATAGACGTA GTAATCGATG TGTTATTCAC
    CCACTTCATT CGTCTTTATC CAGTGAAGAG CAGCAGGCTG TGTTTGTAAA ACCTCCTATG
    GGTGTAACTA AGATTATAAT TTCCACCAAT ATTGCTGAGA CATCCATAAC CATCGATGAT
    GTTGTCTATG TCATTGATTC TGGGAAAATG AAAGAAAAGA GATATGATGC CAGCAAAGGG
    ATGGAAAGTT TAGAGGATAC TTTTGTCTCT CAAGCCAATG CCCTGCAAAG AAAAGGTCGA
    GCAGGTCGTG TAGCGTCTGG GGTCTGCTTC CATTTATTCA CCAGCCATCA CTTTAATCAC
    CAACTTCTAA AGCAGCAGCT ACCAGAAATA CAAAGAGTGC CATTGGAACA GCTCTGTCTG
    AGAATTAAAA TTTTAGAGAT GTTTAGCACT CACAATCTCC AGTCTGTGTT CGCTCGGCTC
    ATTGAACCCC CACACGCTGA TTCTCTCCGT GCCTCAAAAA TACGATTACG AGACTTGGGA
    GCATTAACTC CAGACGAAAC ACTGACCCCT CTAGGGTATC ACTTGGCCTC TTTGCCTGTA
    GATGTGAGAA TCGGCAAACT AATGCTCTTT GGATCTATCT TCCGCTGTTT GGATCCTGCT
    CTCACCATTG CTGCCAGTTT AGCTTTTAAG TCTCCTTTTG TATCTCCCTG GGATAAAAAA
    GAGGAAGCTA ACCAGAAAAA GCTAGAATTT GCATTTGCAA ACAGTGATTA TCTGGCCCTT
    CTACGAGCAT ATAAGGGATG GCAGCTAAGT ATGAAAGAAG GTATGCGTGC AAGTTATAAT
    TACTGCAGAC AAAACTTCTT GTCAGGAAGA GTTCTTCAGG AAATGGCAAG CCTGAAACGA
    CAGTTCACTG AACTGTTATC GGACATAGGC TTTGTTAAGG AGGGACTCCG AGCAAGGGAA
    ATTGAGAAAA GAGCCCAAGG AGGAGGAGAT GGTATATTGG ATGCCACAGG AGAAGAGGCA
    AACTCAAATG CTGAGAACCC CAAGTTGATA TCAGCAATGC TGTGTGCTGC TCTGTATCCA
    AATGTAGTGC AGGTGAAAAG CCCAGAGGGG AAATTTCAGA AGACCAGTAC CGGAGCTGTC
    AGAATGCAGC CAAAATCAGA TGAGTTGAAG TTTGTCACCA AGAACGATGG ATACGTACAC
    ATTCACCCTT CATCGGTGAA CTATCAGGTC AGACACTTTG ACAGTCCCTA CCTGGTATAC
    CACGAGAAGA TCAAAACTAG TCGCGTGTTC ATCCGAGACT GCAGCATGGT GTCTGTGTAC
    CCACTGGTCT TATTTGGAGG AGGCCAAGTG AATGTGCAGC TCCAAAAGGG AGAGTTTGTT
    GTTTCCTTGG ATGATGGTTG GATCCGCTTT GCAGCTGCTT CCCATCAGGT GGCAGAATTA
    GTAAAGGAAC TTCGTTGTGA ACTTGACCAG CTCCTCCAGG ATAAGATAAA AAACCCAAGC
    ATAGATCTGT GCACCTGTCC TCGAGGGTCA CGGATCATCA GCATGATTGT GAAACTTGTC
    ACCACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23962
    Clone ID Related Accession (Same CDS sequence) XM_005212667.3 , NM_001206051.2 , XM_005212666.3
    Accession Version XM_005212666.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative ATP-dependent RNA helicase DHX57 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212666.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAAGAGGTGT TTCTGGGGAA GAAGAAGATG ATGAGCCTGA TTATGACGAC
    GAGCAGTACT GGTCACCTGG ACGAGAAGCT TCCCTTGTTC CTGACTCTGA TCCTTTGGAA
    TGCGCTGGCT CAGGCCTGGT GGAGCCTCAT AACCCAGAAT TTACAGTCTC AGCATTTGCA
    GTGCACAGAC TTTCCAGGTA TGGTTTCCAC ACTGAACACT GTCAGGCAGC GCTGAGGATT
    TGCGATGGAG ACATGGGGGC ATCACTGGAA CGCCTGCTCA CCCAGTGCTT TTCAGAGACA
    TTTGGAGAGA GGATGAAGAT ATCTGAGGCA GTTGCACACA TGAGCTTGGA AGAGTGTGTG
    GAACAGCGAC AGGAAGAGGC ATTTGCTCTC AAGTCAATCT GTGGAGAAAA ATTTATAGAA
    AGAATTCAAA ACAGAGTCTG GACCATTGGA TTAGAACTGG AGTATTTGAC AAGTAAATTC
    TGCAAATTCA AGCAAAAGGA AAGTACCAAA AATATACAGG AGGCTTCACT TGAAGTCTGC
    AAATTTTACC TCAAAGGAAA TTGTAAATTT GGATCAAAGT GCAAATTTAA ACATGAAGTG
    CCCCCAAATC AAATTGTCGG AAGAGTCGAA AGAAATGTAG ATGATTCTCA TCTAAATGCT
    AATGAAGATG CATCCTTTTT ATATGAACTT GAAATTCGAT TTTCTAAAGA CCACAAATAC
    CCCTACCAAG CTCCCCTTGT GGCATTTTAT TCCACCAATG AGAATCTACC TCTGGCTTGT
    CGTTTACATA TTTCTGAGTT CCTTTATGGC AAAGCCTTGA CATTTGCAGA AACTTCAGAA
    CCTGTTGTAT ATTCTTTGAT AACCCTCTTA GAGGAAGAAT CAGAAATAAC CAAGTTACTA
    ACAAATACCC ATCACAAGTA CAGTGTCCCT CCTGTGAACT TTCTGCCAGT ATCACCTGGA
    ACCAGACTAA ATAATCCTGT CTGTCGTAAA CCAGTGATTC CAAGTAATTC GTTTTTTTCG
    AATCAAATTC CAGAAGTTGA AAAAGAATCA GAGCCTGAGG AGGAGACAGA TGAGGATGAA
    GGTCCAGCAC CAGTTATGGT GGAGAATGAA AGCTATGTCA ACCTTAAGAA AAAGATTTCC
    AAAAGATATG ACTGGCAGGC AAAATCAGTA CATGCTGAAA ATGTCAAAAT CTGCAAGCAG
    TTCCGAATAA AACAGGCCTC CAGACAGTTC CAGTCAGTTC TACAAGAAAG ACAGTCGCTC
    CCTGCTTGGG AAGAAAGAGA AAACATTCTC AAATTGTTGA GCAAGCACCA GGTGCTTGTT
    GTAAGTGGTA TGACTGGATG TGGGAAAACC ACACAGATTC CACAGTTTAT TCTGGATGAT
    TCTCTGAATG GACCCCCTGA GAAGGTGGCT AACATCATCT GTACCCAACC CCGACGAATC
    TCTGCCATCT CTGTTGCTGA GCGCGTTGCT AAAGAAAGGG CAGAAAGAGT GGGTCTGACT
    GTGGGATACC AGATCCGGTT AGAAAGTGTC AAGTCCTCAG CCACCAGGTT GTTATACTGC
    ACCACGGGAG TGCTGCTAAG AAGGCTGGAG GGAGACACAG CTCTGCAGGG AGTCACCCAT
    ATCATTGTTG ATGAAGTTCA CGAGAGGACA GAAGAAAGTG ACTTCTTACT ACTAGTTTTG
    AAAGACATTG TGTTGCAGAG ACCAACTCTT CAAGTTATTC TGATGAGTGC AACCTTAAAC
    GCTGAGCTCT TTTCAGAATA TTTTAATTCC TGCCCAGTTA TCACTATACC AGGTCGTACA
    TTTCCTGTTG ATCAGTTTTT TCTGGAAGAT GCAATTGCTG TGACACGGTA TGTGTTGCAG
    GATGGGAGCC CATACGCCCG CTCCATGAAA CAGATGTCAA AGGAGAAGCT CAAGGCCAGG
    CGCAGCAGAA CCGCATTTGA GGAGGTGGAG GAGGACCTGC GTCTCTCCCT GCACCTCCAG
    GATCAGGATT CTGTCAAAGA TGCGGTACCA GATCAACAAT TAGATTTTAA ACAGCTCCTT
    GCCCGCTATC AAGGAGTTAG CAAATCAGTC ATCAAAACAA TGTCCGTTAT GGATTTTGAA
    AAGGTTAATC TTGAATTAAT CGAGGCCTTG TTAGAGTGGA TTGTGGACGG AAAGCACTCC
    TACCCTCCAG GTGCTATACT TGTATTTTTA CCGGGACTAG CAGAAATCAA AATGCTGTAT
    GAACAGCTCC AGTCTAATTC TCTTTTCAAC AATAGACGTA GTAATCGATG TGTTATTCAC
    CCACTTCATT CGTCTTTATC CAGTGAAGAG CAGCAGGCTG TGTTTGTAAA ACCTCCTATG
    GGTGTAACTA AGATTATAAT TTCCACCAAT ATTGCTGAGA CATCCATAAC CATCGATGAT
    GTTGTCTATG TCATTGATTC TGGGAAAATG AAAGAAAAGA GATATGATGC CAGCAAAGGG
    ATGGAAAGTT TAGAGGATAC TTTTGTCTCT CAAGCCAATG CCCTGCAAAG AAAAGGTCGA
    GCAGGTCGTG TAGCGTCTGG GGTCTGCTTC CATTTATTCA CCAGCCATCA CTTTAATCAC
    CAACTTCTAA AGCAGCAGCT ACCAGAAATA CAAAGAGTGC CATTGGAACA GCTCTGTCTG
    AGAATTAAAA TTTTAGAGAT GTTTAGCACT CACAATCTCC AGTCTGTGTT CGCTCGGCTC
    ATTGAACCCC CACACGCTGA TTCTCTCCGT GCCTCAAAAA TACGATTACG AGACTTGGGA
    GCATTAACTC CAGACGAAAC ACTGACCCCT CTAGGGTATC ACTTGGCCTC TTTGCCTGTA
    GATGTGAGAA TCGGCAAACT AATGCTCTTT GGATCTATCT TCCGCTGTTT GGATCCTGCT
    CTCACCATTG CTGCCAGTTT AGCTTTTAAG TCTCCTTTTG TATCTCCCTG GGATAAAAAA
    GAGGAAGCTA ACCAGAAAAA GCTAGAATTT GCATTTGCAA ACAGTGATTA TCTGGCCCTT
    CTACGAGCAT ATAAGGGATG GCAGCTAAGT ATGAAAGAAG GTATGCGTGC AAGTTATAAT
    TACTGCAGAC AAAACTTCTT GTCAGGAAGA GTTCTTCAGG AAATGGCAAG CCTGAAACGA
    CAGTTCACTG AACTGTTATC GGACATAGGC TTTGTTAAGG AGGGACTCCG AGCAAGGGAA
    ATTGAGAAAA GAGCCCAAGG AGGAGGAGAT GGTATATTGG ATGCCACAGG AGAAGAGGCA
    AACTCAAATG CTGAGAACCC CAAGTTGATA TCAGCAATGC TGTGTGCTGC TCTGTATCCA
    AATGTAGTGC AGGTGAAAAG CCCAGAGGGG AAATTTCAGA AGACCAGTAC CGGAGCTGTC
    AGAATGCAGC CAAAATCAGA TGAGTTGAAG TTTGTCACCA AGAACGATGG ATACGTACAC
    ATTCACCCTT CATCGGTGAA CTATCAGGTC AGACACTTTG ACAGTCCCTA CCTGGTATAC
    CACGAGAAGA TCAAAACTAG TCGCGTGTTC ATCCGAGACT GCAGCATGGT GTCTGTGTAC
    CCACTGGTCT TATTTGGAGG AGGCCAAGTG AATGTGCAGC TCCAAAAGGG AGAGTTTGTT
    GTTTCCTTGG ATGATGGTTG GATCCGCTTT GCAGCTGCTT CCCATCAGGT GGCAGAATTA
    GTAAAGGAAC TTCGTTGTGA ACTTGACCAG CTCCTCCAGG ATAAGATAAA AAACCCAAGC
    ATAGATCTGT GCACCTGTCC TCGAGGGTCA CGGATCATCA GCATGATTGT GAAACTTGTC
    ACCACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212723.1
    CDS87..4238
    Translation

    Target ORF information:

    RefSeq Version XM_005212666.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DEAH-box helicase 57 (DHX57), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212666.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAAGAGGTGT TTCTGGGGAA GAAGAAGATG ATGAGCCTGA TTATGACGAC
    GAGCAGTACT GGTCACCTGG ACGAGAAGCT TCCCTTGTTC CTGACTCTGA TCCTTTGGAA
    TGCGCTGGCT CAGGCCTGGT GGAGCCTCAT AACCCAGAAT TTACAGTCTC AGCATTTGCA
    GTGCACAGAC TTTCCAGGTA TGGTTTCCAC ACTGAACACT GTCAGGCAGC GCTGAGGATT
    TGCGATGGAG ACATGGGGGC ATCACTGGAA CGCCTGCTCA CCCAGTGCTT TTCAGAGACA
    TTTGGAGAGA GGATGAAGAT ATCTGAGGCA GTTGCACACA TGAGCTTGGA AGAGTGTGTG
    GAACAGCGAC AGGAAGAGGC ATTTGCTCTC AAGTCAATCT GTGGAGAAAA ATTTATAGAA
    AGAATTCAAA ACAGAGTCTG GACCATTGGA TTAGAACTGG AGTATTTGAC AAGTAAATTC
    TGCAAATTCA AGCAAAAGGA AAGTACCAAA AATATACAGG AGGCTTCACT TGAAGTCTGC
    AAATTTTACC TCAAAGGAAA TTGTAAATTT GGATCAAAGT GCAAATTTAA ACATGAAGTG
    CCCCCAAATC AAATTGTCGG AAGAGTCGAA AGAAATGTAG ATGATTCTCA TCTAAATGCT
    AATGAAGATG CATCCTTTTT ATATGAACTT GAAATTCGAT TTTCTAAAGA CCACAAATAC
    CCCTACCAAG CTCCCCTTGT GGCATTTTAT TCCACCAATG AGAATCTACC TCTGGCTTGT
    CGTTTACATA TTTCTGAGTT CCTTTATGGC AAAGCCTTGA CATTTGCAGA AACTTCAGAA
    CCTGTTGTAT ATTCTTTGAT AACCCTCTTA GAGGAAGAAT CAGAAATAAC CAAGTTACTA
    ACAAATACCC ATCACAAGTA CAGTGTCCCT CCTGTGAACT TTCTGCCAGT ATCACCTGGA
    ACCAGACTAA ATAATCCTGT CTGTCGTAAA CCAGTGATTC CAAGTAATTC GTTTTTTTCG
    AATCAAATTC CAGAAGTTGA AAAAGAATCA GAGCCTGAGG AGGAGACAGA TGAGGATGAA
    GGTCCAGCAC CAGTTATGGT GGAGAATGAA AGCTATGTCA ACCTTAAGAA AAAGATTTCC
    AAAAGATATG ACTGGCAGGC AAAATCAGTA CATGCTGAAA ATGTCAAAAT CTGCAAGCAG
    TTCCGAATAA AACAGGCCTC CAGACAGTTC CAGTCAGTTC TACAAGAAAG ACAGTCGCTC
    CCTGCTTGGG AAGAAAGAGA AAACATTCTC AAATTGTTGA GCAAGCACCA GGTGCTTGTT
    GTAAGTGGTA TGACTGGATG TGGGAAAACC ACACAGATTC CACAGTTTAT TCTGGATGAT
    TCTCTGAATG GACCCCCTGA GAAGGTGGCT AACATCATCT GTACCCAACC CCGACGAATC
    TCTGCCATCT CTGTTGCTGA GCGCGTTGCT AAAGAAAGGG CAGAAAGAGT GGGTCTGACT
    GTGGGATACC AGATCCGGTT AGAAAGTGTC AAGTCCTCAG CCACCAGGTT GTTATACTGC
    ACCACGGGAG TGCTGCTAAG AAGGCTGGAG GGAGACACAG CTCTGCAGGG AGTCACCCAT
    ATCATTGTTG ATGAAGTTCA CGAGAGGACA GAAGAAAGTG ACTTCTTACT ACTAGTTTTG
    AAAGACATTG TGTTGCAGAG ACCAACTCTT CAAGTTATTC TGATGAGTGC AACCTTAAAC
    GCTGAGCTCT TTTCAGAATA TTTTAATTCC TGCCCAGTTA TCACTATACC AGGTCGTACA
    TTTCCTGTTG ATCAGTTTTT TCTGGAAGAT GCAATTGCTG TGACACGGTA TGTGTTGCAG
    GATGGGAGCC CATACGCCCG CTCCATGAAA CAGATGTCAA AGGAGAAGCT CAAGGCCAGG
    CGCAGCAGAA CCGCATTTGA GGAGGTGGAG GAGGACCTGC GTCTCTCCCT GCACCTCCAG
    GATCAGGATT CTGTCAAAGA TGCGGTACCA GATCAACAAT TAGATTTTAA ACAGCTCCTT
    GCCCGCTATC AAGGAGTTAG CAAATCAGTC ATCAAAACAA TGTCCGTTAT GGATTTTGAA
    AAGGTTAATC TTGAATTAAT CGAGGCCTTG TTAGAGTGGA TTGTGGACGG AAAGCACTCC
    TACCCTCCAG GTGCTATACT TGTATTTTTA CCGGGACTAG CAGAAATCAA AATGCTGTAT
    GAACAGCTCC AGTCTAATTC TCTTTTCAAC AATAGACGTA GTAATCGATG TGTTATTCAC
    CCACTTCATT CGTCTTTATC CAGTGAAGAG CAGCAGGCTG TGTTTGTAAA ACCTCCTATG
    GGTGTAACTA AGATTATAAT TTCCACCAAT ATTGCTGAGA CATCCATAAC CATCGATGAT
    GTTGTCTATG TCATTGATTC TGGGAAAATG AAAGAAAAGA GATATGATGC CAGCAAAGGG
    ATGGAAAGTT TAGAGGATAC TTTTGTCTCT CAAGCCAATG CCCTGCAAAG AAAAGGTCGA
    GCAGGTCGTG TAGCGTCTGG GGTCTGCTTC CATTTATTCA CCAGCCATCA CTTTAATCAC
    CAACTTCTAA AGCAGCAGCT ACCAGAAATA CAAAGAGTGC CATTGGAACA GCTCTGTCTG
    AGAATTAAAA TTTTAGAGAT GTTTAGCACT CACAATCTCC AGTCTGTGTT CGCTCGGCTC
    ATTGAACCCC CACACGCTGA TTCTCTCCGT GCCTCAAAAA TACGATTACG AGACTTGGGA
    GCATTAACTC CAGACGAAAC ACTGACCCCT CTAGGGTATC ACTTGGCCTC TTTGCCTGTA
    GATGTGAGAA TCGGCAAACT AATGCTCTTT GGATCTATCT TCCGCTGTTT GGATCCTGCT
    CTCACCATTG CTGCCAGTTT AGCTTTTAAG TCTCCTTTTG TATCTCCCTG GGATAAAAAA
    GAGGAAGCTA ACCAGAAAAA GCTAGAATTT GCATTTGCAA ACAGTGATTA TCTGGCCCTT
    CTACGAGCAT ATAAGGGATG GCAGCTAAGT ATGAAAGAAG GTATGCGTGC AAGTTATAAT
    TACTGCAGAC AAAACTTCTT GTCAGGAAGA GTTCTTCAGG AAATGGCAAG CCTGAAACGA
    CAGTTCACTG AACTGTTATC GGACATAGGC TTTGTTAAGG AGGGACTCCG AGCAAGGGAA
    ATTGAGAAAA GAGCCCAAGG AGGAGGAGAT GGTATATTGG ATGCCACAGG AGAAGAGGCA
    AACTCAAATG CTGAGAACCC CAAGTTGATA TCAGCAATGC TGTGTGCTGC TCTGTATCCA
    AATGTAGTGC AGGTGAAAAG CCCAGAGGGG AAATTTCAGA AGACCAGTAC CGGAGCTGTC
    AGAATGCAGC CAAAATCAGA TGAGTTGAAG TTTGTCACCA AGAACGATGG ATACGTACAC
    ATTCACCCTT CATCGGTGAA CTATCAGGTC AGACACTTTG ACAGTCCCTA CCTGGTATAC
    CACGAGAAGA TCAAAACTAG TCGCGTGTTC ATCCGAGACT GCAGCATGGT GTCTGTGTAC
    CCACTGGTCT TATTTGGAGG AGGCCAAGTG AATGTGCAGC TCCAAAAGGG AGAGTTTGTT
    GTTTCCTTGG ATGATGGTTG GATCCGCTTT GCAGCTGCTT CCCATCAGGT GGCAGAATTA
    GTAAAGGAAC TTCGTTGTGA ACTTGACCAG CTCCTCCAGG ATAAGATAAA AAACCCAAGC
    ATAGATCTGT GCACCTGTCC TCGAGGGTCA CGGATCATCA GCATGATTGT GAAACTTGTC
    ACCACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36966
    Clone ID Related Accession (Same CDS sequence) NM_001206051.1 , XM_005212667.4 , NM_001206051.3 , XM_005212666.4
    Accession Version XM_005212667.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative ATP-dependent RNA helicase DHX57 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212667.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAAGAGGTGT TTCTGGGGAA GAAGAAGATG ATGAGCCTGA TTATGACGAC
    GAGCAGTACT GGTCACCTGG ACGAGAAGCT TCCCTTGTTC CTGACTCTGA TCCTTTGGAA
    TGCGCTGGCT CAGGCCTGGT GGAGCCTCAT AACCCAGAAT TTACAGTCTC AGCATTTGCA
    GTGCACAGAC TTTCCAGGTA TGGTTTCCAC ACTGAACACT GTCAGGCAGC GCTGAGGATT
    TGCGATGGAG ACATGGGGGC ATCACTGGAA CGCCTGCTCA CCCAGTGCTT TTCAGAGACA
    TTTGGAGAGA GGATGAAGAT ATCTGAGGCA GTTGCACACA TGAGCTTGGA AGAGTGTGTG
    GAACAGCGAC AGGAAGAGGC ATTTGCTCTC AAGTCAATCT GTGGAGAAAA ATTTATAGAA
    AGAATTCAAA ACAGAGTCTG GACCATTGGA TTAGAACTGG AGTATTTGAC AAGTAAATTC
    TGCAAATTCA AGCAAAAGGA AAGTACCAAA AATATACAGG AGGCTTCACT TGAAGTCTGC
    AAATTTTACC TCAAAGGAAA TTGTAAATTT GGATCAAAGT GCAAATTTAA ACATGAAGTG
    CCCCCAAATC AAATTGTCGG AAGAGTCGAA AGAAATGTAG ATGATTCTCA TCTAAATGCT
    AATGAAGATG CATCCTTTTT ATATGAACTT GAAATTCGAT TTTCTAAAGA CCACAAATAC
    CCCTACCAAG CTCCCCTTGT GGCATTTTAT TCCACCAATG AGAATCTACC TCTGGCTTGT
    CGTTTACATA TTTCTGAGTT CCTTTATGGC AAAGCCTTGA CATTTGCAGA AACTTCAGAA
    CCTGTTGTAT ATTCTTTGAT AACCCTCTTA GAGGAAGAAT CAGAAATAAC CAAGTTACTA
    ACAAATACCC ATCACAAGTA CAGTGTCCCT CCTGTGAACT TTCTGCCAGT ATCACCTGGA
    ACCAGACTAA ATAATCCTGT CTGTCGTAAA CCAGTGATTC CAAGTAATTC GTTTTTTTCG
    AATCAAATTC CAGAAGTTGA AAAAGAATCA GAGCCTGAGG AGGAGACAGA TGAGGATGAA
    GGTCCAGCAC CAGTTATGGT GGAGAATGAA AGCTATGTCA ACCTTAAGAA AAAGATTTCC
    AAAAGATATG ACTGGCAGGC AAAATCAGTA CATGCTGAAA ATGTCAAAAT CTGCAAGCAG
    TTCCGAATAA AACAGGCCTC CAGACAGTTC CAGTCAGTTC TACAAGAAAG ACAGTCGCTC
    CCTGCTTGGG AAGAAAGAGA AAACATTCTC AAATTGTTGA GCAAGCACCA GGTGCTTGTT
    GTAAGTGGTA TGACTGGATG TGGGAAAACC ACACAGATTC CACAGTTTAT TCTGGATGAT
    TCTCTGAATG GACCCCCTGA GAAGGTGGCT AACATCATCT GTACCCAACC CCGACGAATC
    TCTGCCATCT CTGTTGCTGA GCGCGTTGCT AAAGAAAGGG CAGAAAGAGT GGGTCTGACC
    GTGGGATACC AGATCCGGTT AGAAAGTGTC AAGTCCTCAG CCACCAGGTT GTTATACTGC
    ACCACGGGAG TGCTGCTAAG AAGGCTGGAG GGAGACACAG CTCTGCAGGG AGTCACCCAT
    ATCATTGTTG ATGAAGTTCA CGAGAGGACA GAAGAAAGTG ACTTCTTACT ACTAGTTTTG
    AAAGACATTG TGTTGCAGAG ACCAACTCTT CAAGTTATTC TGATGAGTGC AACCTTAAAC
    GCTGAGCTCT TTTCAGAATA TTTTAATTCC TGCCCAGTTA TCACTATACC AGGTCGTACA
    TTTCCTGTTG ATCAGTTTTT TCTGGAAGAT GCAATTGCTG TGACACGGTA TGTGTTGCAG
    GATGGGAGCC CATACGCCCG CTCCATGAAA CAGATGTCAA AGGAGAAGCT CAAGGCCAGG
    CGCAGCAGAA CCGCATTTGA GGAGGTGGAG GAGGACCTGC GTCTCTCCCT GCACCTCCAG
    GATCAGGATT CTGTCAAAGA TGCGGTACCA GATCAACAAT TAGATTTTAA ACAGCTCCTT
    GCCCGCTATC AAGGAGTTAG CAAATCAGTC ATCAAAACAA TGTCCGTTAT GGATTTTGAA
    AAGGTTAATC TTGAATTAAT CGAGGCCTTG TTAGAGTGGA TTGTGGACGG AAAGCACTCC
    TACCCTCCAG GTGCTATACT TGTATTTTTA CCGGGACTAG CAGAAATCAA AATGCTGTAT
    GAACAGCTCC AGTCTAATTC TCTTTTCAAC AATAGACGTA GTAATCGATG TGTTATTCAC
    CCACTTCATT CGTCTTTATC CAGTGAAGAG CAGCAGGCTG TGTTTGTAAA ACCTCCTATG
    GGTGTAACTA AGATTATAAT TTCCACCAAT ATTGCTGAGA CATCCATAAC CATCGATGAT
    GTTGTCTATG TCATTGATTC TGGGAAAATG AAAGAAAAGA GATATGATGC CAGCAAAGGG
    ATGGAAAGTT TAGAGGATAC TTTTGTCTCT CAAGCCAATG CCCTGCAAAG AAAAGGTCGA
    GCAGGTCGTG TAGCGTCTGG GGTCTGCTTC CATTTATTCA CCAGCCATCA CTTTAATCAC
    CAACTTCTAA AGCAGCAGCT ACCAGAAATA CAAAGAGTGC CATTGGAACA GCTCTGTCTG
    AGAATTAAAA TTTTAGAGAT GTTTAGCACT CACAATCTCC AGTCTGTGTT CGCTCGGCTC
    ATTGAACCCC CACACGCTGA TTCTCTCCGT GCCTCAAAAA TACGATTACG AGACTTGGGA
    GCATTAACTC CAGACGAAAC ACTGACCCCT CTAGGGTATC ACTTGGCCTC TTTGCCTGTA
    GATGTGAGAA TCGGCAAACT AATGCTCTTT GGATCTATCT TCCGCTGTTT GGATCCTGCT
    CTCACCATTG CTGCCAGTTT AGCTTTTAAG TCTCCTTTTG TATCTCCCTG GGATAAAAAA
    GAGGAAGCTA ACCAGAAAAA GCTAGAATTT GCATTTGCAA ACAGTGATTA TCTGGCCCTT
    CTACGAGCAT ATAAGGGATG GCAGCTAAGT ATGAAAGAAG GTATGCGTGC AAGTTATAAT
    TACTGCAGAC AAAACTTCTT GTCAGGAAGA GTTCTTCAGG AAATGGCAAG CCTGAAACGA
    CAGTTCACTG AACTGTTATC GGACATAGGC TTTGTTAAGG AGGGACTCCG AGCAAGGGAA
    ATTGAGAAAA GAGCCCAAGG AGGAGGAGAT GGTATATTGG ATGCCACAGG AGAAGAGGCA
    AACTCAAATG CTGAGAACCC CAAGTTGATA TCAGCAATGC TGTGTGCTGC TTTGTATCCA
    AATGTAGTGC AGGTGAAAAG CCCAGAGGGG AAATTTCAGA AGACCAGTAC CGGAGCTGTC
    AGAATGCAGC CAAAATCAGA TGAGTTGAAG TTTGTCACCA AGAACGATGG ATACGTACAC
    ATTCACCCTT CATCGGTGAA CTATCAGGTC AGACACTTTG ACAGTCCCTA CCTGGTATAC
    CACGAGAAGA TCAAAACTAG TCGCGTGTTC ATCCGAGACT GCAGCATGGT GTCTGTGTAC
    CCACTGGTCT TATTTGGAGG AGGCCAAGTG AATGTGCAGC TCCAAAAGGG AGAGTTTGTT
    GTTTCCTTGG ATGATGGTTG GATCCGCTTT GCAGCTGCTT CCCATCAGGT GGCAGAATTA
    GTAAAGGAAC TTCGTTGTGA ACTTGACCAG CTCCTCCAGG ATAAGATAAA AAACCCAAGC
    ATAGATCTGT GCACCTGTCC TCGAGGGTCA CGGATCATCA GCATGATTGT GAAACTTGTC
    ACCACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212724.1
    CDS281..4432
    Translation

    Target ORF information:

    RefSeq Version XM_005212667.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DExH-box helicase 57 (DHX57), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212667.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAAGAGGTGT TTCTGGGGAA GAAGAAGATG ATGAGCCTGA TTATGACGAC
    GAGCAGTACT GGTCACCTGG ACGAGAAGCT TCCCTTGTTC CTGACTCTGA TCCTTTGGAA
    TGCGCTGGCT CAGGCCTGGT GGAGCCTCAT AACCCAGAAT TTACAGTCTC AGCATTTGCA
    GTGCACAGAC TTTCCAGGTA TGGTTTCCAC ACTGAACACT GTCAGGCAGC GCTGAGGATT
    TGCGATGGAG ACATGGGGGC ATCACTGGAA CGCCTGCTCA CCCAGTGCTT TTCAGAGACA
    TTTGGAGAGA GGATGAAGAT ATCTGAGGCA GTTGCACACA TGAGCTTGGA AGAGTGTGTG
    GAACAGCGAC AGGAAGAGGC ATTTGCTCTC AAGTCAATCT GTGGAGAAAA ATTTATAGAA
    AGAATTCAAA ACAGAGTCTG GACCATTGGA TTAGAACTGG AGTATTTGAC AAGTAAATTC
    TGCAAATTCA AGCAAAAGGA AAGTACCAAA AATATACAGG AGGCTTCACT TGAAGTCTGC
    AAATTTTACC TCAAAGGAAA TTGTAAATTT GGATCAAAGT GCAAATTTAA ACATGAAGTG
    CCCCCAAATC AAATTGTCGG AAGAGTCGAA AGAAATGTAG ATGATTCTCA TCTAAATGCT
    AATGAAGATG CATCCTTTTT ATATGAACTT GAAATTCGAT TTTCTAAAGA CCACAAATAC
    CCCTACCAAG CTCCCCTTGT GGCATTTTAT TCCACCAATG AGAATCTACC TCTGGCTTGT
    CGTTTACATA TTTCTGAGTT CCTTTATGGC AAAGCCTTGA CATTTGCAGA AACTTCAGAA
    CCTGTTGTAT ATTCTTTGAT AACCCTCTTA GAGGAAGAAT CAGAAATAAC CAAGTTACTA
    ACAAATACCC ATCACAAGTA CAGTGTCCCT CCTGTGAACT TTCTGCCAGT ATCACCTGGA
    ACCAGACTAA ATAATCCTGT CTGTCGTAAA CCAGTGATTC CAAGTAATTC GTTTTTTTCG
    AATCAAATTC CAGAAGTTGA AAAAGAATCA GAGCCTGAGG AGGAGACAGA TGAGGATGAA
    GGTCCAGCAC CAGTTATGGT GGAGAATGAA AGCTATGTCA ACCTTAAGAA AAAGATTTCC
    AAAAGATATG ACTGGCAGGC AAAATCAGTA CATGCTGAAA ATGTCAAAAT CTGCAAGCAG
    TTCCGAATAA AACAGGCCTC CAGACAGTTC CAGTCAGTTC TACAAGAAAG ACAGTCGCTC
    CCTGCTTGGG AAGAAAGAGA AAACATTCTC AAATTGTTGA GCAAGCACCA GGTGCTTGTT
    GTAAGTGGTA TGACTGGATG TGGGAAAACC ACACAGATTC CACAGTTTAT TCTGGATGAT
    TCTCTGAATG GACCCCCTGA GAAGGTGGCT AACATCATCT GTACCCAACC CCGACGAATC
    TCTGCCATCT CTGTTGCTGA GCGCGTTGCT AAAGAAAGGG CAGAAAGAGT GGGTCTGACC
    GTGGGATACC AGATCCGGTT AGAAAGTGTC AAGTCCTCAG CCACCAGGTT GTTATACTGC
    ACCACGGGAG TGCTGCTAAG AAGGCTGGAG GGAGACACAG CTCTGCAGGG AGTCACCCAT
    ATCATTGTTG ATGAAGTTCA CGAGAGGACA GAAGAAAGTG ACTTCTTACT ACTAGTTTTG
    AAAGACATTG TGTTGCAGAG ACCAACTCTT CAAGTTATTC TGATGAGTGC AACCTTAAAC
    GCTGAGCTCT TTTCAGAATA TTTTAATTCC TGCCCAGTTA TCACTATACC AGGTCGTACA
    TTTCCTGTTG ATCAGTTTTT TCTGGAAGAT GCAATTGCTG TGACACGGTA TGTGTTGCAG
    GATGGGAGCC CATACGCCCG CTCCATGAAA CAGATGTCAA AGGAGAAGCT CAAGGCCAGG
    CGCAGCAGAA CCGCATTTGA GGAGGTGGAG GAGGACCTGC GTCTCTCCCT GCACCTCCAG
    GATCAGGATT CTGTCAAAGA TGCGGTACCA GATCAACAAT TAGATTTTAA ACAGCTCCTT
    GCCCGCTATC AAGGAGTTAG CAAATCAGTC ATCAAAACAA TGTCCGTTAT GGATTTTGAA
    AAGGTTAATC TTGAATTAAT CGAGGCCTTG TTAGAGTGGA TTGTGGACGG AAAGCACTCC
    TACCCTCCAG GTGCTATACT TGTATTTTTA CCGGGACTAG CAGAAATCAA AATGCTGTAT
    GAACAGCTCC AGTCTAATTC TCTTTTCAAC AATAGACGTA GTAATCGATG TGTTATTCAC
    CCACTTCATT CGTCTTTATC CAGTGAAGAG CAGCAGGCTG TGTTTGTAAA ACCTCCTATG
    GGTGTAACTA AGATTATAAT TTCCACCAAT ATTGCTGAGA CATCCATAAC CATCGATGAT
    GTTGTCTATG TCATTGATTC TGGGAAAATG AAAGAAAAGA GATATGATGC CAGCAAAGGG
    ATGGAAAGTT TAGAGGATAC TTTTGTCTCT CAAGCCAATG CCCTGCAAAG AAAAGGTCGA
    GCAGGTCGTG TAGCGTCTGG GGTCTGCTTC CATTTATTCA CCAGCCATCA CTTTAATCAC
    CAACTTCTAA AGCAGCAGCT ACCAGAAATA CAAAGAGTGC CATTGGAACA GCTCTGTCTG
    AGAATTAAAA TTTTAGAGAT GTTTAGCACT CACAATCTCC AGTCTGTGTT CGCTCGGCTC
    ATTGAACCCC CACACGCTGA TTCTCTCCGT GCCTCAAAAA TACGATTACG AGACTTGGGA
    GCATTAACTC CAGACGAAAC ACTGACCCCT CTAGGGTATC ACTTGGCCTC TTTGCCTGTA
    GATGTGAGAA TCGGCAAACT AATGCTCTTT GGATCTATCT TCCGCTGTTT GGATCCTGCT
    CTCACCATTG CTGCCAGTTT AGCTTTTAAG TCTCCTTTTG TATCTCCCTG GGATAAAAAA
    GAGGAAGCTA ACCAGAAAAA GCTAGAATTT GCATTTGCAA ACAGTGATTA TCTGGCCCTT
    CTACGAGCAT ATAAGGGATG GCAGCTAAGT ATGAAAGAAG GTATGCGTGC AAGTTATAAT
    TACTGCAGAC AAAACTTCTT GTCAGGAAGA GTTCTTCAGG AAATGGCAAG CCTGAAACGA
    CAGTTCACTG AACTGTTATC GGACATAGGC TTTGTTAAGG AGGGACTCCG AGCAAGGGAA
    ATTGAGAAAA GAGCCCAAGG AGGAGGAGAT GGTATATTGG ATGCCACAGG AGAAGAGGCA
    AACTCAAATG CTGAGAACCC CAAGTTGATA TCAGCAATGC TGTGTGCTGC TTTGTATCCA
    AATGTAGTGC AGGTGAAAAG CCCAGAGGGG AAATTTCAGA AGACCAGTAC CGGAGCTGTC
    AGAATGCAGC CAAAATCAGA TGAGTTGAAG TTTGTCACCA AGAACGATGG ATACGTACAC
    ATTCACCCTT CATCGGTGAA CTATCAGGTC AGACACTTTG ACAGTCCCTA CCTGGTATAC
    CACGAGAAGA TCAAAACTAG TCGCGTGTTC ATCCGAGACT GCAGCATGGT GTCTGTGTAC
    CCACTGGTCT TATTTGGAGG AGGCCAAGTG AATGTGCAGC TCCAAAAGGG AGAGTTTGTT
    GTTTCCTTGG ATGATGGTTG GATCCGCTTT GCAGCTGCTT CCCATCAGGT GGCAGAATTA
    GTAAAGGAAC TTCGTTGTGA ACTTGACCAG CTCCTCCAGG ATAAGATAAA AAACCCAAGC
    ATAGATCTGT GCACCTGTCC TCGAGGGTCA CGGATCATCA GCATGATTGT GAAACTTGTC
    ACCACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36966
    Clone ID Related Accession (Same CDS sequence) NM_001206051.1 , XM_005212667.4 , NM_001206051.3 , XM_005212666.4
    Accession Version XM_005212666.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative ATP-dependent RNA helicase DHX57 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212666.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAAGAGGTGT TTCTGGGGAA GAAGAAGATG ATGAGCCTGA TTATGACGAC
    GAGCAGTACT GGTCACCTGG ACGAGAAGCT TCCCTTGTTC CTGACTCTGA TCCTTTGGAA
    TGCGCTGGCT CAGGCCTGGT GGAGCCTCAT AACCCAGAAT TTACAGTCTC AGCATTTGCA
    GTGCACAGAC TTTCCAGGTA TGGTTTCCAC ACTGAACACT GTCAGGCAGC GCTGAGGATT
    TGCGATGGAG ACATGGGGGC ATCACTGGAA CGCCTGCTCA CCCAGTGCTT TTCAGAGACA
    TTTGGAGAGA GGATGAAGAT ATCTGAGGCA GTTGCACACA TGAGCTTGGA AGAGTGTGTG
    GAACAGCGAC AGGAAGAGGC ATTTGCTCTC AAGTCAATCT GTGGAGAAAA ATTTATAGAA
    AGAATTCAAA ACAGAGTCTG GACCATTGGA TTAGAACTGG AGTATTTGAC AAGTAAATTC
    TGCAAATTCA AGCAAAAGGA AAGTACCAAA AATATACAGG AGGCTTCACT TGAAGTCTGC
    AAATTTTACC TCAAAGGAAA TTGTAAATTT GGATCAAAGT GCAAATTTAA ACATGAAGTG
    CCCCCAAATC AAATTGTCGG AAGAGTCGAA AGAAATGTAG ATGATTCTCA TCTAAATGCT
    AATGAAGATG CATCCTTTTT ATATGAACTT GAAATTCGAT TTTCTAAAGA CCACAAATAC
    CCCTACCAAG CTCCCCTTGT GGCATTTTAT TCCACCAATG AGAATCTACC TCTGGCTTGT
    CGTTTACATA TTTCTGAGTT CCTTTATGGC AAAGCCTTGA CATTTGCAGA AACTTCAGAA
    CCTGTTGTAT ATTCTTTGAT AACCCTCTTA GAGGAAGAAT CAGAAATAAC CAAGTTACTA
    ACAAATACCC ATCACAAGTA CAGTGTCCCT CCTGTGAACT TTCTGCCAGT ATCACCTGGA
    ACCAGACTAA ATAATCCTGT CTGTCGTAAA CCAGTGATTC CAAGTAATTC GTTTTTTTCG
    AATCAAATTC CAGAAGTTGA AAAAGAATCA GAGCCTGAGG AGGAGACAGA TGAGGATGAA
    GGTCCAGCAC CAGTTATGGT GGAGAATGAA AGCTATGTCA ACCTTAAGAA AAAGATTTCC
    AAAAGATATG ACTGGCAGGC AAAATCAGTA CATGCTGAAA ATGTCAAAAT CTGCAAGCAG
    TTCCGAATAA AACAGGCCTC CAGACAGTTC CAGTCAGTTC TACAAGAAAG ACAGTCGCTC
    CCTGCTTGGG AAGAAAGAGA AAACATTCTC AAATTGTTGA GCAAGCACCA GGTGCTTGTT
    GTAAGTGGTA TGACTGGATG TGGGAAAACC ACACAGATTC CACAGTTTAT TCTGGATGAT
    TCTCTGAATG GACCCCCTGA GAAGGTGGCT AACATCATCT GTACCCAACC CCGACGAATC
    TCTGCCATCT CTGTTGCTGA GCGCGTTGCT AAAGAAAGGG CAGAAAGAGT GGGTCTGACC
    GTGGGATACC AGATCCGGTT AGAAAGTGTC AAGTCCTCAG CCACCAGGTT GTTATACTGC
    ACCACGGGAG TGCTGCTAAG AAGGCTGGAG GGAGACACAG CTCTGCAGGG AGTCACCCAT
    ATCATTGTTG ATGAAGTTCA CGAGAGGACA GAAGAAAGTG ACTTCTTACT ACTAGTTTTG
    AAAGACATTG TGTTGCAGAG ACCAACTCTT CAAGTTATTC TGATGAGTGC AACCTTAAAC
    GCTGAGCTCT TTTCAGAATA TTTTAATTCC TGCCCAGTTA TCACTATACC AGGTCGTACA
    TTTCCTGTTG ATCAGTTTTT TCTGGAAGAT GCAATTGCTG TGACACGGTA TGTGTTGCAG
    GATGGGAGCC CATACGCCCG CTCCATGAAA CAGATGTCAA AGGAGAAGCT CAAGGCCAGG
    CGCAGCAGAA CCGCATTTGA GGAGGTGGAG GAGGACCTGC GTCTCTCCCT GCACCTCCAG
    GATCAGGATT CTGTCAAAGA TGCGGTACCA GATCAACAAT TAGATTTTAA ACAGCTCCTT
    GCCCGCTATC AAGGAGTTAG CAAATCAGTC ATCAAAACAA TGTCCGTTAT GGATTTTGAA
    AAGGTTAATC TTGAATTAAT CGAGGCCTTG TTAGAGTGGA TTGTGGACGG AAAGCACTCC
    TACCCTCCAG GTGCTATACT TGTATTTTTA CCGGGACTAG CAGAAATCAA AATGCTGTAT
    GAACAGCTCC AGTCTAATTC TCTTTTCAAC AATAGACGTA GTAATCGATG TGTTATTCAC
    CCACTTCATT CGTCTTTATC CAGTGAAGAG CAGCAGGCTG TGTTTGTAAA ACCTCCTATG
    GGTGTAACTA AGATTATAAT TTCCACCAAT ATTGCTGAGA CATCCATAAC CATCGATGAT
    GTTGTCTATG TCATTGATTC TGGGAAAATG AAAGAAAAGA GATATGATGC CAGCAAAGGG
    ATGGAAAGTT TAGAGGATAC TTTTGTCTCT CAAGCCAATG CCCTGCAAAG AAAAGGTCGA
    GCAGGTCGTG TAGCGTCTGG GGTCTGCTTC CATTTATTCA CCAGCCATCA CTTTAATCAC
    CAACTTCTAA AGCAGCAGCT ACCAGAAATA CAAAGAGTGC CATTGGAACA GCTCTGTCTG
    AGAATTAAAA TTTTAGAGAT GTTTAGCACT CACAATCTCC AGTCTGTGTT CGCTCGGCTC
    ATTGAACCCC CACACGCTGA TTCTCTCCGT GCCTCAAAAA TACGATTACG AGACTTGGGA
    GCATTAACTC CAGACGAAAC ACTGACCCCT CTAGGGTATC ACTTGGCCTC TTTGCCTGTA
    GATGTGAGAA TCGGCAAACT AATGCTCTTT GGATCTATCT TCCGCTGTTT GGATCCTGCT
    CTCACCATTG CTGCCAGTTT AGCTTTTAAG TCTCCTTTTG TATCTCCCTG GGATAAAAAA
    GAGGAAGCTA ACCAGAAAAA GCTAGAATTT GCATTTGCAA ACAGTGATTA TCTGGCCCTT
    CTACGAGCAT ATAAGGGATG GCAGCTAAGT ATGAAAGAAG GTATGCGTGC AAGTTATAAT
    TACTGCAGAC AAAACTTCTT GTCAGGAAGA GTTCTTCAGG AAATGGCAAG CCTGAAACGA
    CAGTTCACTG AACTGTTATC GGACATAGGC TTTGTTAAGG AGGGACTCCG AGCAAGGGAA
    ATTGAGAAAA GAGCCCAAGG AGGAGGAGAT GGTATATTGG ATGCCACAGG AGAAGAGGCA
    AACTCAAATG CTGAGAACCC CAAGTTGATA TCAGCAATGC TGTGTGCTGC TTTGTATCCA
    AATGTAGTGC AGGTGAAAAG CCCAGAGGGG AAATTTCAGA AGACCAGTAC CGGAGCTGTC
    AGAATGCAGC CAAAATCAGA TGAGTTGAAG TTTGTCACCA AGAACGATGG ATACGTACAC
    ATTCACCCTT CATCGGTGAA CTATCAGGTC AGACACTTTG ACAGTCCCTA CCTGGTATAC
    CACGAGAAGA TCAAAACTAG TCGCGTGTTC ATCCGAGACT GCAGCATGGT GTCTGTGTAC
    CCACTGGTCT TATTTGGAGG AGGCCAAGTG AATGTGCAGC TCCAAAAGGG AGAGTTTGTT
    GTTTCCTTGG ATGATGGTTG GATCCGCTTT GCAGCTGCTT CCCATCAGGT GGCAGAATTA
    GTAAAGGAAC TTCGTTGTGA ACTTGACCAG CTCCTCCAGG ATAAGATAAA AAACCCAAGC
    ATAGATCTGT GCACCTGTCC TCGAGGGTCA CGGATCATCA GCATGATTGT GAAACTTGTC
    ACCACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212723.1
    CDS87..4238
    Translation

    Target ORF information:

    RefSeq Version XM_005212666.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DExH-box helicase 57 (DHX57), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212666.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTTCTT CAGTAAGAAG GAAAGGCAAG CCAGGGAAAG GAGGTGGAAA AGGGTCTTCC 
    AGAGGAGGAA GAGGAGGCAG GAGCCATGCT AGTAAATCTC ATGGGAGTGG CAGCAGTGGC
    GGTGGTGTTG GTGGCAACAG AAAGGCATCA AGTAGAATCT GGGATGATGG CGATGACTTT
    TGTATCTTCA GTGAATCAAG GCATTCATCC AGACCTAGTA ACAGTAGCGT GAGAAAAGGG
    GATGGACGCC CCAAATGGAA ACCCGAAGCC AAAGTGCCCC TGCAGACTCT ACATATGACT
    TCTGAGAATC AGGAAAAAGT GAAAGCTCTT CTCCGAGACT TGCAGGAACA AGATGCGGAT
    GCTGGATCTG AAAGAGGTGT TTCTGGGGAA GAAGAAGATG ATGAGCCTGA TTATGACGAC
    GAGCAGTACT GGTCACCTGG ACGAGAAGCT TCCCTTGTTC CTGACTCTGA TCCTTTGGAA
    TGCGCTGGCT CAGGCCTGGT GGAGCCTCAT AACCCAGAAT TTACAGTCTC AGCATTTGCA
    GTGCACAGAC TTTCCAGGTA TGGTTTCCAC ACTGAACACT GTCAGGCAGC GCTGAGGATT
    TGCGATGGAG ACATGGGGGC ATCACTGGAA CGCCTGCTCA CCCAGTGCTT TTCAGAGACA
    TTTGGAGAGA GGATGAAGAT ATCTGAGGCA GTTGCACACA TGAGCTTGGA AGAGTGTGTG
    GAACAGCGAC AGGAAGAGGC ATTTGCTCTC AAGTCAATCT GTGGAGAAAA ATTTATAGAA
    AGAATTCAAA ACAGAGTCTG GACCATTGGA TTAGAACTGG AGTATTTGAC AAGTAAATTC
    TGCAAATTCA AGCAAAAGGA AAGTACCAAA AATATACAGG AGGCTTCACT TGAAGTCTGC
    AAATTTTACC TCAAAGGAAA TTGTAAATTT GGATCAAAGT GCAAATTTAA ACATGAAGTG
    CCCCCAAATC AAATTGTCGG AAGAGTCGAA AGAAATGTAG ATGATTCTCA TCTAAATGCT
    AATGAAGATG CATCCTTTTT ATATGAACTT GAAATTCGAT TTTCTAAAGA CCACAAATAC
    CCCTACCAAG CTCCCCTTGT GGCATTTTAT TCCACCAATG AGAATCTACC TCTGGCTTGT
    CGTTTACATA TTTCTGAGTT CCTTTATGGC AAAGCCTTGA CATTTGCAGA AACTTCAGAA
    CCTGTTGTAT ATTCTTTGAT AACCCTCTTA GAGGAAGAAT CAGAAATAAC CAAGTTACTA
    ACAAATACCC ATCACAAGTA CAGTGTCCCT CCTGTGAACT TTCTGCCAGT ATCACCTGGA
    ACCAGACTAA ATAATCCTGT CTGTCGTAAA CCAGTGATTC CAAGTAATTC GTTTTTTTCG
    AATCAAATTC CAGAAGTTGA AAAAGAATCA GAGCCTGAGG AGGAGACAGA TGAGGATGAA
    GGTCCAGCAC CAGTTATGGT GGAGAATGAA AGCTATGTCA ACCTTAAGAA AAAGATTTCC
    AAAAGATATG ACTGGCAGGC AAAATCAGTA CATGCTGAAA ATGTCAAAAT CTGCAAGCAG
    TTCCGAATAA AACAGGCCTC CAGACAGTTC CAGTCAGTTC TACAAGAAAG ACAGTCGCTC
    CCTGCTTGGG AAGAAAGAGA AAACATTCTC AAATTGTTGA GCAAGCACCA GGTGCTTGTT
    GTAAGTGGTA TGACTGGATG TGGGAAAACC ACACAGATTC CACAGTTTAT TCTGGATGAT
    TCTCTGAATG GACCCCCTGA GAAGGTGGCT AACATCATCT GTACCCAACC CCGACGAATC
    TCTGCCATCT CTGTTGCTGA GCGCGTTGCT AAAGAAAGGG CAGAAAGAGT GGGTCTGACC
    GTGGGATACC AGATCCGGTT AGAAAGTGTC AAGTCCTCAG CCACCAGGTT GTTATACTGC
    ACCACGGGAG TGCTGCTAAG AAGGCTGGAG GGAGACACAG CTCTGCAGGG AGTCACCCAT
    ATCATTGTTG ATGAAGTTCA CGAGAGGACA GAAGAAAGTG ACTTCTTACT ACTAGTTTTG
    AAAGACATTG TGTTGCAGAG ACCAACTCTT CAAGTTATTC TGATGAGTGC AACCTTAAAC
    GCTGAGCTCT TTTCAGAATA TTTTAATTCC TGCCCAGTTA TCACTATACC AGGTCGTACA
    TTTCCTGTTG ATCAGTTTTT TCTGGAAGAT GCAATTGCTG TGACACGGTA TGTGTTGCAG
    GATGGGAGCC CATACGCCCG CTCCATGAAA CAGATGTCAA AGGAGAAGCT CAAGGCCAGG
    CGCAGCAGAA CCGCATTTGA GGAGGTGGAG GAGGACCTGC GTCTCTCCCT GCACCTCCAG
    GATCAGGATT CTGTCAAAGA TGCGGTACCA GATCAACAAT TAGATTTTAA ACAGCTCCTT
    GCCCGCTATC AAGGAGTTAG CAAATCAGTC ATCAAAACAA TGTCCGTTAT GGATTTTGAA
    AAGGTTAATC TTGAATTAAT CGAGGCCTTG TTAGAGTGGA TTGTGGACGG AAAGCACTCC
    TACCCTCCAG GTGCTATACT TGTATTTTTA CCGGGACTAG CAGAAATCAA AATGCTGTAT
    GAACAGCTCC AGTCTAATTC TCTTTTCAAC AATAGACGTA GTAATCGATG TGTTATTCAC
    CCACTTCATT CGTCTTTATC CAGTGAAGAG CAGCAGGCTG TGTTTGTAAA ACCTCCTATG
    GGTGTAACTA AGATTATAAT TTCCACCAAT ATTGCTGAGA CATCCATAAC CATCGATGAT
    GTTGTCTATG TCATTGATTC TGGGAAAATG AAAGAAAAGA GATATGATGC CAGCAAAGGG
    ATGGAAAGTT TAGAGGATAC TTTTGTCTCT CAAGCCAATG CCCTGCAAAG AAAAGGTCGA
    GCAGGTCGTG TAGCGTCTGG GGTCTGCTTC CATTTATTCA CCAGCCATCA CTTTAATCAC
    CAACTTCTAA AGCAGCAGCT ACCAGAAATA CAAAGAGTGC CATTGGAACA GCTCTGTCTG
    AGAATTAAAA TTTTAGAGAT GTTTAGCACT CACAATCTCC AGTCTGTGTT CGCTCGGCTC
    ATTGAACCCC CACACGCTGA TTCTCTCCGT GCCTCAAAAA TACGATTACG AGACTTGGGA
    GCATTAACTC CAGACGAAAC ACTGACCCCT CTAGGGTATC ACTTGGCCTC TTTGCCTGTA
    GATGTGAGAA TCGGCAAACT AATGCTCTTT GGATCTATCT TCCGCTGTTT GGATCCTGCT
    CTCACCATTG CTGCCAGTTT AGCTTTTAAG TCTCCTTTTG TATCTCCCTG GGATAAAAAA
    GAGGAAGCTA ACCAGAAAAA GCTAGAATTT GCATTTGCAA ACAGTGATTA TCTGGCCCTT
    CTACGAGCAT ATAAGGGATG GCAGCTAAGT ATGAAAGAAG GTATGCGTGC AAGTTATAAT
    TACTGCAGAC AAAACTTCTT GTCAGGAAGA GTTCTTCAGG AAATGGCAAG CCTGAAACGA
    CAGTTCACTG AACTGTTATC GGACATAGGC TTTGTTAAGG AGGGACTCCG AGCAAGGGAA
    ATTGAGAAAA GAGCCCAAGG AGGAGGAGAT GGTATATTGG ATGCCACAGG AGAAGAGGCA
    AACTCAAATG CTGAGAACCC CAAGTTGATA TCAGCAATGC TGTGTGCTGC TTTGTATCCA
    AATGTAGTGC AGGTGAAAAG CCCAGAGGGG AAATTTCAGA AGACCAGTAC CGGAGCTGTC
    AGAATGCAGC CAAAATCAGA TGAGTTGAAG TTTGTCACCA AGAACGATGG ATACGTACAC
    ATTCACCCTT CATCGGTGAA CTATCAGGTC AGACACTTTG ACAGTCCCTA CCTGGTATAC
    CACGAGAAGA TCAAAACTAG TCGCGTGTTC ATCCGAGACT GCAGCATGGT GTCTGTGTAC
    CCACTGGTCT TATTTGGAGG AGGCCAAGTG AATGTGCAGC TCCAAAAGGG AGAGTTTGTT
    GTTTCCTTGG ATGATGGTTG GATCCGCTTT GCAGCTGCTT CCCATCAGGT GGCAGAATTA
    GTAAAGGAAC TTCGTTGTGA ACTTGACCAG CTCCTCCAGG ATAAGATAAA AAACCCAAGC
    ATAGATCTGT GCACCTGTCC TCGAGGGTCA CGGATCATCA GCATGATTGT GAAACTTGTC
    ACCACACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.