ME1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ME1
Entrez Gene ID	540985
Full Name	malic enzyme 1
General protein information	Preferred Names malic enzyme 1 Names NADP-dependent malic enzyme cytosolic malic enzyme 1 malic enzyme 1, NADP(+)-dependent, cytoplasmic (cytosolic, soluble)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144853.1	NP_001138325.1	NADP-dependent malic enzyme
XM_024996809.1	XP_024852577.1	NADP-dependent malic enzyme isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ME1	NP_002386.1
Pan troglodytes (chimpanzee)	ME1	XP_518610.3
Bos taurus (cattle)	ME1	NP_001138325.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000184	XP_310951.5
Neurospora crassa	NCU03651	XP_960731.2
Oryza sativa (rice)	Os05g0186300	NP_001054832.1
Canis lupus familiaris (dog)	ME1	XP_532217.4
Magnaporthe oryzae (rice blast fungus)	MGG_08173	XP_003715145.1
Arabidopsis thaliana (thale cress)	NADP-ME4	NP_178093.1
Drosophila melanogaster (fruit fly)	Men	NP_731739.1
Oryza sativa (rice)	Os01g0188400	NP_001042257.1
Mus musculus (house mouse)	Me1	NP_032641.2
Rattus norvegicus (Norway rat)	Me1	NP_036732.2
Gallus gallus (chicken)	ME1	NP_989634.1
Xenopus tropicalis (tropical clawed frog)	me1	XP_002938500.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of polymorphisms in the malic enzyme 1, NADP(+)-dependent, cytosolic and nuclear receptor subfamily 0, group B, member 2 genes and their associations with meat and carcass quality traits in commercial Angus cattle.
Gill JL, Bishop SC, McCorquodale C, Williams JL, Wiener P
Animal genetics43(1)88-92(2012 Feb)

Cloning and polymorphisms of the 3' untranslated region of malic enzyme gene in Chinese red cattle.
Zhou GL, Zhang GL, Cao Y, Jin HG
Meat science89(1)72-5(2011 Sep)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Six novel SNPs, five in ME1 and one in NR0B2, were identified as candidates that have effects on meat and carcass quality traits.
Title: Identification of polymorphisms in the malic enzyme 1, NADP(+)-dependent, cytosolic and nuclear receptor subfamily 0, group B, member 2 genes and their associations with meat and carcass quality traits in commercial Angus cattle.

ME1-Dra I genotypes had a significant effect on cooking loss.
Title: Cloning and polymorphisms of the 3' untranslated region of malic enzyme gene in Chinese red cattle.

The following ME1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ME1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa39997	NM_001144853.1 Latest version!	Bos taurus malic enzyme 1 (ME1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa237887	XM_024996809.1 Latest version!	Bos taurus malic enzyme 1 (ME1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ME1 ( NM_001144853.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001138325.1 Bos taurus malic enzyme 1 (ME1), mRNA.

Price & Availability↑

CloneID	OBa39997
Clone ID Related Accession (Same CDS sequence)	NM_001144853.1
Accession Version	NM_001144853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	NADP-dependent malic enzyme
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ515746.1. On Jan 27, 2009 this sequence version replaced XM_613987.4. ##Evidence-Data-START## Transcript exon combination :: FJ515746.1, FJ515745.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGGCACCA GCTGTGTGGA TAGCACGTGG AGCAAAGCCA GGCGTGGTCA TGGTAGGGCC 
AGACCCAGAG ACTTGGCTTT TACCCTGGAA GAGAGGCAGC AACTGAACAT TCATGGCCTG 
TTGCCACCCT CCTTCATCAG TCAGGATGTC CAGGTCCTTC GAGTACTTAA GAATTTTGAG 
CGTCTGAACT CTGATTTTGA CAGGTATCTT CTCTTAATGG ATCTTCAAGA TAGAAACGAA 
AAGCTCTTCT ATAAAGTGCT GATGTCTGAC ATTGAGAAAT TCATGCCTAT CGTTTATACT 
CCCACTGTGG GTCTAGCTTG CCAACAATAT AGTTTAGCAT TTCGGAAGCC AAGAGGTCTC 
TTTATTAGTA TCCATGATCG AGGACATATT TCTTCTGTTC TTAATGCATG GCCAGAAGAT 
GTCATCAAGG CTATTGTGGT GACTGATGGA GAACGTATTC TTGGCTTGGG AGACCTTGGC 
TGTAATGGGA TGGGCATCCC CGTGGGTAAA CTGGCTCTGT ATACAGCTTG TGGAGGGATG 
AACCCTCAAC AGTGTTTGCC TGTCATGCTG GATGTGGGAA CTGAAAATGA GGAGTTACTT 
AAAGATCCAT TGTACATTGG ACTACGGCAG AGAAGAGTAA GAGGTCCTGA ATATGATGAT 
TTTTTGGATG AATTCATGGA GGCAGTTTCT CTCAAGTATG GCATGAACTG CCTTATTCAG 
TTTGAAGATT TTGCCAATAT AAATGCATTT CGGCTCCTGA GAAAGTATCA AAACCAGTAT 
TGCACATTCA ATGATGATAT CCAAGGAACA GCATCTGTTG CAGTTGCCGG CATCCTTGCA 
GCCCTTCGAA TAACCAAGAA CAAGCTGTCT GAGCAAACAG TTCTATTCCA AGGAGCTGGA 
GAGGCTGCCT TAGGGATTGC ACACCTGATT GTCATGGCCA TGGAGAAAGA AGGTTTATCA 
AAGGAGCAAG CCATAGAGAA GATATGGTTG GTTGACTCGA AAGGATTAAT AGTTAAGGGA 
CGTGCTGCCT TAACACAAGA GATAGAGGAA TTTGCCCATG AACATAAAGA AATGAAGAAC 
CTTGAAGCCA TCGTTCAAGA TATAAAACCA ACTGCCCTCA TTGGAGTTGC TGCGATTGGT 
GGTGCGTTCT CAGAACAAAT TATCAAAGAC ATGGCAGCCT TCAATGAACG GCCTATTATT 
TTTGCCTTGA GTAATCCAAC TAGTAAAGCA GAATGTACTG CAGAGCAGTG CTATAAACTG 
ACCAAGGGTC GCGCCATTTT TGCCAGCGGC AGTCCTTTTG ATCCCGTTAC CCTTCCGAGT 
GGAAAGTCTC TTTATCCTGG CCAAGGCAAC AATTCCTACG TGTTCCCTGG AGTTGCTCTT 
GGTGTTGTGG CGTGTGGACT GAGGCATATC ACGGATAAGA TTTTTCTCAC CACTGCTGAG 
GTCATAGCTC AGCAAGTATC AGATAAACAC TTGGAGGAGG GCCGGCTTTA TCCTCCTTTG 
GACACCATTA GAGATGTTTC TCTGAAAATT GCAGAAAAGA TTGTGAAAGA TGCATACCAA 
GAAAAGACAG CCACAGTTTA TCCTGAACCC CCAAACAAGG AAGCATTTGT CCGCTCTCAG 
ATGTATAGCA CTGACTATGA CCAGATTCTA CCTGATTGTT ATCCTTGGCC TAAAGAGGCC 
CAGAAAATAC AGACCAAACT TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138325.1
CDS	99..1805
Translation	MGTSCVDSTWSKARRGHGRARPRDLAFTLEERQQLNIHGLLPPSFISQDVQVLRVLKNFERLNSDFDRYLLLMDLQDRNEKLFYKVLMSDIEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDRGHISSVLNAWPEDVIKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGMNPQQCLPVMLDVGTENEELLKDPLYIGLRQRRVRGPEYDDFLDEFMEAVSLKYGMNCLIQFEDFANINAFRLLRKYQNQYCTFNDDIQGTASVAVAGILAALRITKNKLSEQTVLFQGAGEAALGIAHLIVMAMEKEGLSKEQAIEKIWLVDSKGLIVKGRAALTQEIEEFAHEHKEMKNLEAIVQDIKPTALIGVAAIGGAFSEQIIKDMAAFNERPIIFALSNPTSKAECTAEQCYKLTKGRAIFASGSPFDPVTLPSGKSLYPGQGNNSYVFPGVALGVVACGLRHITDKIFLTTAEVIAQQVSDKHLEEGRLYPPLDTIRDVSLKIAEKIVKDAYQEKTATVYPEPPNKEAFVRSQMYSTDYDQILPDCYPWPKEAQKIQTKLD

Target ORF information:

RefSeq Version	NM_001144853.1
Organism	Bos taurus(cattle)
Definition	Bos taurus malic enzyme 1 (ME1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144853.1

ORF Insert Sequence:

ATGGGCACCA GCTGTGTGGA TAGCACGTGG AGCAAAGCCA GGCGTGGTCA TGGTAGGGCC 
AGACCCAGAG ACTTGGCTTT TACCCTGGAA GAGAGGCAGC AACTGAACAT TCATGGCCTG 
TTGCCACCCT CCTTCATCAG TCAGGATGTC CAGGTCCTTC GAGTACTTAA GAATTTTGAG 
CGTCTGAACT CTGATTTTGA CAGGTATCTT CTCTTAATGG ATCTTCAAGA TAGAAACGAA 
AAGCTCTTCT ATAAAGTGCT GATGTCTGAC ATTGAGAAAT TCATGCCTAT CGTTTATACT 
CCCACTGTGG GTCTAGCTTG CCAACAATAT AGTTTAGCAT TTCGGAAGCC AAGAGGTCTC 
TTTATTAGTA TCCATGATCG AGGACATATT TCTTCTGTTC TTAATGCATG GCCAGAAGAT 
GTCATCAAGG CTATTGTGGT GACTGATGGA GAACGTATTC TTGGCTTGGG AGACCTTGGC 
TGTAATGGGA TGGGCATCCC CGTGGGTAAA CTGGCTCTGT ATACAGCTTG TGGAGGGATG 
AACCCTCAAC AGTGTTTGCC TGTCATGCTG GATGTGGGAA CTGAAAATGA GGAGTTACTT 
AAAGATCCAT TGTACATTGG ACTACGGCAG AGAAGAGTAA GAGGTCCTGA ATATGATGAT 
TTTTTGGATG AATTCATGGA GGCAGTTTCT CTCAAGTATG GCATGAACTG CCTTATTCAG 
TTTGAAGATT TTGCCAATAT AAATGCATTT CGGCTCCTGA GAAAGTATCA AAACCAGTAT 
TGCACATTCA ATGATGATAT CCAAGGAACA GCATCTGTTG CAGTTGCCGG CATCCTTGCA 
GCCCTTCGAA TAACCAAGAA CAAGCTGTCT GAGCAAACAG TTCTATTCCA AGGAGCTGGA 
GAGGCTGCCT TAGGGATTGC ACACCTGATT GTCATGGCCA TGGAGAAAGA AGGTTTATCA 
AAGGAGCAAG CCATAGAGAA GATATGGTTG GTTGACTCGA AAGGATTAAT AGTTAAGGGA 
CGTGCTGCCT TAACACAAGA GATAGAGGAA TTTGCCCATG AACATAAAGA AATGAAGAAC 
CTTGAAGCCA TCGTTCAAGA TATAAAACCA ACTGCCCTCA TTGGAGTTGC TGCGATTGGT 
GGTGCGTTCT CAGAACAAAT TATCAAAGAC ATGGCAGCCT TCAATGAACG GCCTATTATT 
TTTGCCTTGA GTAATCCAAC TAGTAAAGCA GAATGTACTG CAGAGCAGTG CTATAAACTG 
ACCAAGGGTC GCGCCATTTT TGCCAGCGGC AGTCCTTTTG ATCCCGTTAC CCTTCCGAGT 
GGAAAGTCTC TTTATCCTGG CCAAGGCAAC AATTCCTACG TGTTCCCTGG AGTTGCTCTT 
GGTGTTGTGG CGTGTGGACT GAGGCATATC ACGGATAAGA TTTTTCTCAC CACTGCTGAG 
GTCATAGCTC AGCAAGTATC AGATAAACAC TTGGAGGAGG GCCGGCTTTA TCCTCCTTTG 
GACACCATTA GAGATGTTTC TCTGAAAATT GCAGAAAAGA TTGTGAAAGA TGCATACCAA 
GAAAAGACAG CCACAGTTTA TCCTGAACCC CCAAACAAGG AAGCATTTGT CCGCTCTCAG 
ATGTATAGCA CTGACTATGA CCAGATTCTA CCTGATTGTT ATCCTTGGCC TAAAGAGGCC 
CAGAAAATAC AGACCAAACT TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ME1 ( XM_024996809.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852577.1 Bos taurus malic enzyme 1 (ME1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa237887
Clone ID Related Accession (Same CDS sequence)	XM_024996809.1
Accession Version	XM_024996809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NADP-dependent malic enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCC GGACCCCTCG CCGCCGCCAC ACCCCCCAGC GCGGCTACGC CGTCACCCGG 
AACCCCCACC TCAACAAGGA CTTGGCTTTT ACCCTGGAAG AGAGGCAGCA ACTGAACATT 
CATGGCCTGT TGCCACCCTC CTTCATCAGT CAGGATGTCC AGGTCCTTCG AGTACTTAAG 
AATTTTGAGC GTCTGAACTC TGATTTTGAC AGGTATCTTC TCTTAATGGA TCTTCAAGAT 
AGAAACGAAA AGCTCTTCTA TAAAGTGCTG ATGTCTGACA TTGAGAAATT CATGCCTATC 
GTTTATACTC CCACTGTGGG TCTAGCTTGC CAACAATATA GTTTAGCATT TCGGAAGCCA 
AGAGGTCTCT TTATTAGTAT CCATGATCGA GGACATATTT CTTCTGTTCT TAATGCATGG 
CCAGAAGATG TCATCAAGGC TATTGTGGTG ACTGATGGAG AACGTATTCT TGGCTTGGGA 
GACCTTGGCT GTAATGGGAT GGGCATCCCC GTGGGTAAAC TGGCTCTGTA TACAGCTTGT 
GGAGGGATGA ACCCTCAACA GTGTTTGCCT GTCATGCTGG ATGTGGGAAC GGAAAATGAG 
GAGTTACTTA AAGATCCATT GTACATTGGA CTACGGCAGA GAAGAGTAAG AGGTCCTGAA 
TATGATGATT TTTTGGATGA ATTCATGGAG GCAGTTTCTC TCAAGTATGG CATGAACTGC 
CTTATTCAGT TTGAAGATTT TGCCAATATA AATGCATTTC GGCTCCTGAG AAAGTATCAA 
AACCAGTATT GCACATTCAA TGATGATATC CAAGGAACAG CATCTGTTGC AGTTGCCGGC 
ATCCTTGCAG CCCTTCGAAT AACCAAGAAC AAGCTGTCTG ATCAAACAGT TCTATTCCAA 
GGAGCTGGAG AGGCTGCCTT AGGGATTGCA CACCTGATTG TCATGGCCAT GGAGAAAGAA 
GGTTTATCAA AGGAGCAAGC CATAGAGAAG ATATGGTTGG TTGACTCGAA AGGATTAATA 
GTTAAGGGAC GTGCTGCCTT AACACAAGAG AAAGAGGAAT TTGCCCATGA ACATAAAGAA 
ATGAAGAACC TTGAAGCCAT CGTTCAAGAT ATAAAACCAA CTGCCCTCAT TGGAGTTGCT 
GCGATTGGTG GTGCGTTCTC AGAACAAATT ATCAAAGACA TGGCAGCCTT CAATGAACGG 
CCTATTATTT TTGCCTTGAG TAATCCAACT AGTAAAGCAG AATGTACTGC AGAGCAGTGC 
TATAAACTGA CCAAGGGTCG CGCCATTTTT GCCAGCGGCA GTCCTTTTGA TCCCGTTACC 
CTTCCGAGTG GAAAGACTCT TTATCCTGGC CAAGGCAACA ATTCCTACGT GTTCCCTGGA 
GTTGCTCTTG GTGTTGTGGC GTGTGGACTG AGGCATATCA CGGATAAGAT TTTTCTCACC 
ACTGCTGAGG TCATAGCTCA GCAAGTATCA GATAAACACT TGGAGGAGGG CCGGCTTTAT 
CCTCCTTTGG ACACCATTAG AGATGTTTCT CTGAAAATTG CAGAAAAGAT TGTGAAAGAT 
GCATACCAAG AAAAGACAGC CACAGTTTAT CCTGAACCCC CAAACAAGGA AGCATTTGTC 
CGCTCTCAGA TGTATAGCAC TGACTATGAC CAGATTCTAC CTGATTGTTA TCCTTGGCCT 
AAAGAGGCCC AGAAAATACA GACCAAACTT GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852577.1
CDS	111..1826
Translation	MEPRTPRRRHTPQRGYAVTRNPHLNKDLAFTLEERQQLNIHGLLPPSFISQDVQVLRVLKNFERLNSDFDRYLLLMDLQDRNEKLFYKVLMSDIEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDRGHISSVLNAWPEDVIKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGMNPQQCLPVMLDVGTENEELLKDPLYIGLRQRRVRGPEYDDFLDEFMEAVSLKYGMNCLIQFEDFANINAFRLLRKYQNQYCTFNDDIQGTASVAVAGILAALRITKNKLSDQTVLFQGAGEAALGIAHLIVMAMEKEGLSKEQAIEKIWLVDSKGLIVKGRAALTQEKEEFAHEHKEMKNLEAIVQDIKPTALIGVAAIGGAFSEQIIKDMAAFNERPIIFALSNPTSKAECTAEQCYKLTKGRAIFASGSPFDPVTLPSGKTLYPGQGNNSYVFPGVALGVVACGLRHITDKIFLTTAEVIAQQVSDKHLEEGRLYPPLDTIRDVSLKIAEKIVKDAYQEKTATVYPEPPNKEAFVRSQMYSTDYDQILPDCYPWPKEAQKIQTKLD

Target ORF information:

RefSeq Version	XM_024996809.1
Organism	Bos taurus(cattle)
Definition	Bos taurus malic enzyme 1 (ME1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996809.1

ORF Insert Sequence:

ATGGAGCCCC GGACCCCTCG CCGCCGCCAC ACCCCCCAGC GCGGCTACGC CGTCACCCGG 
AACCCCCACC TCAACAAGGA CTTGGCTTTT ACCCTGGAAG AGAGGCAGCA ACTGAACATT 
CATGGCCTGT TGCCACCCTC CTTCATCAGT CAGGATGTCC AGGTCCTTCG AGTACTTAAG 
AATTTTGAGC GTCTGAACTC TGATTTTGAC AGGTATCTTC TCTTAATGGA TCTTCAAGAT 
AGAAACGAAA AGCTCTTCTA TAAAGTGCTG ATGTCTGACA TTGAGAAATT CATGCCTATC 
GTTTATACTC CCACTGTGGG TCTAGCTTGC CAACAATATA GTTTAGCATT TCGGAAGCCA 
AGAGGTCTCT TTATTAGTAT CCATGATCGA GGACATATTT CTTCTGTTCT TAATGCATGG 
CCAGAAGATG TCATCAAGGC TATTGTGGTG ACTGATGGAG AACGTATTCT TGGCTTGGGA 
GACCTTGGCT GTAATGGGAT GGGCATCCCC GTGGGTAAAC TGGCTCTGTA TACAGCTTGT 
GGAGGGATGA ACCCTCAACA GTGTTTGCCT GTCATGCTGG ATGTGGGAAC GGAAAATGAG 
GAGTTACTTA AAGATCCATT GTACATTGGA CTACGGCAGA GAAGAGTAAG AGGTCCTGAA 
TATGATGATT TTTTGGATGA ATTCATGGAG GCAGTTTCTC TCAAGTATGG CATGAACTGC 
CTTATTCAGT TTGAAGATTT TGCCAATATA AATGCATTTC GGCTCCTGAG AAAGTATCAA 
AACCAGTATT GCACATTCAA TGATGATATC CAAGGAACAG CATCTGTTGC AGTTGCCGGC 
ATCCTTGCAG CCCTTCGAAT AACCAAGAAC AAGCTGTCTG ATCAAACAGT TCTATTCCAA 
GGAGCTGGAG AGGCTGCCTT AGGGATTGCA CACCTGATTG TCATGGCCAT GGAGAAAGAA 
GGTTTATCAA AGGAGCAAGC CATAGAGAAG ATATGGTTGG TTGACTCGAA AGGATTAATA 
GTTAAGGGAC GTGCTGCCTT AACACAAGAG AAAGAGGAAT TTGCCCATGA ACATAAAGAA 
ATGAAGAACC TTGAAGCCAT CGTTCAAGAT ATAAAACCAA CTGCCCTCAT TGGAGTTGCT 
GCGATTGGTG GTGCGTTCTC AGAACAAATT ATCAAAGACA TGGCAGCCTT CAATGAACGG 
CCTATTATTT TTGCCTTGAG TAATCCAACT AGTAAAGCAG AATGTACTGC AGAGCAGTGC 
TATAAACTGA CCAAGGGTCG CGCCATTTTT GCCAGCGGCA GTCCTTTTGA TCCCGTTACC 
CTTCCGAGTG GAAAGACTCT TTATCCTGGC CAAGGCAACA ATTCCTACGT GTTCCCTGGA 
GTTGCTCTTG GTGTTGTGGC GTGTGGACTG AGGCATATCA CGGATAAGAT TTTTCTCACC 
ACTGCTGAGG TCATAGCTCA GCAAGTATCA GATAAACACT TGGAGGAGGG CCGGCTTTAT 
CCTCCTTTGG ACACCATTAG AGATGTTTCT CTGAAAATTG CAGAAAAGAT TGTGAAAGAT 
GCATACCAAG AAAAGACAGC CACAGTTTAT CCTGAACCCC CAAACAAGGA AGCATTTGTC 
CGCTCTCAGA TGTATAGCAC TGACTATGAC CAGATTCTAC CTGATTGTTA TCCTTGGCCT 
AAAGAGGCCC AGAAAATACA GACCAAACTT GACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of polymorphisms in the malic enzyme 1, NADP(+)-dependent, cytosolic and nuclear receptor subfamily 0, group B, member 2 genes and their associations with meat and carcass quality traits in commercial Angus cattle.
Animal genetics43(1)88-92(2012 Feb)
Gill JL,Bishop SC,McCorquodale C,Williams JL,Wiener P

Cloning and polymorphisms of the 3' untranslated region of malic enzyme gene in Chinese red cattle.
Meat science89(1)72-5(2011 Sep)
Zhou GL,Zhang GL,Cao Y,Jin HG

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ME1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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