IBTK cDNA ORF clone, Bos taurus(cattle)

The following IBTK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IBTK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa216382 NM_001206040.2
Latest version!
Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa238923 NM_001206040.3
Latest version!
Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237881 XM_005210695.4
Latest version!
Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237882 XM_005210696.4
Latest version!
Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa54896 XM_010808382.2
Latest version!
Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa54897 XM_010808383.2
Latest version!
Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa40388 NM_001206040.1
Latest version!
Bos taurus inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa22493 XM_005210695.3
Latest version!
Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa22494 XM_005210696.3
Latest version!
Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa216382
    Clone ID Related Accession (Same CDS sequence) NM_001206040.2
    Accession Version NM_001206040.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product inhibitor of Bruton tyrosine kinase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02025483.1 and DAAA02025482.1. On Jun 30, 2017 this sequence version replaced NM_001206040.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCCT CCATCCCTGA CTGCACATCA AAGTGTCGCT CCCTGACGCA TGCTTTGGAT 
    GTCCTTTCTG TGGTAACGAA GGGGAGTGAA AACCAGATTA AGGCCTTTCT ATCCAGTCAT
    TGTCACAATG CTGCAACCAT CAGGGATGCC TTTGGTCGGA ATGCCCTCCA CCTTGTTGCC
    TCCTGTGGGA AAAAAGGAGT GTTAGATTGG CTTATTGAGA AAGGAGTGGA TCCCTCGGTG
    AAAGACAAGG AGTCAGGATG GACAGCTTTG CATAGAAGCA TTTTTTATGG GCATATTGAT
    TGTGTTTGGT CTCTACTGAA GCATGGTGTT AATCTGTATA TTCAAGATAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGATAGA CCAACTCATG TAGTATTCAA AAATACTGAT
    CCTACAGATG TCTACACTTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ATCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGAT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCACTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC
    TGTGGTCATG GTCCTGGAGG ACGATTGGGC CATGGAGATG AGCAGACATG CTTGGTCCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATAAT TGTTCTCAAG TAGCGGCTGC TAAGGATCAT
    ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACATTTG GTCTAAATAT TTTTCATCAA
    TTAGGACTCA TTCCTCCGCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC
    CTGAAAGGAA GGACAATCAT TGGAGTAGCA GCTGGCAGGT TTCATACTGT GCTGTGGACT
    AGAGAGGCTG TTTACACTAT GGGACTAAAT GGTGGACAAT TGGGTTATTT ACTAGATCCC
    AATGGAGAAA AGTGTGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGACATC
    ACTCTGTCTT TGGTTGCTGC TAGTGATGGG GCTACTGTCT GTGTTACCAC AAAGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGCTGGCTT CTAAACAACT GAACTTGAAA
    AAGGTTCTTG TATCTGGGGG GCATATGGAA TACAAAGTTG ATCCTGAACA TTTGAAAGAA
    AATGGGGGTC AGAAAATTTG CATTCTTGCA ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCTGTCA ACAGTTCTAT GAAGCAGTGT CGATGGGCTT ATCCACGCCA AGTCTTCATT
    TCTGATATTG CTTTAAGTAG AAATGAAATT CTTTTTGTCA CACAAGATGG AGAGGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAGAAAG AGTTCTGAAA AGAAAGAGAT TTTATCAAAC
    CTTCATAATT CCTCACCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATATGAAAGA
    ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT
    GGATGCAACT TTGCAGTCTT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAACT
    GTGTCCTCCT CATCCTTTTT TGAGGAGTTT GGCAAGCTGT TGAGGGAAAC GGATGAAATG
    GACAACATCC ATGATGTGAC ATTTCAAATT GGCAGTAGAC TCTTCCCTGC ACATAAATAT
    ATTTTGGCAG TGCGTTCTGA CTTTTTCCAG AAATTGTTTC TTTCGGATGG TACCGCATTA
    GACTTCACAG ATGTTTACCG GAAAGATGAA GATTCTACAG GCTGCCATCT CTTTGTGGTA
    GAGAAGGTTC ATCCTGACCT GTTTGAATAC CTTTTGCAGT TTATATACAC AGATACTTGT
    GACTTTTTAA CTCATGGCTT CAAACCAAGA CTAAATTTAA ACAGAAAACC AGAAGGATAT
    CCAAGCACTC CAAACTGTCA TTCGAATAAA ATGAATTCCC ATGAAGATAA TCAGAAGTCA
    GCATTTGAAG CTTACAAAAG TAATCAAGCT CATACAATTA GTGAAAAGCA GAAGAGTAAA
    TCCAAATCTT CCAAAAAAGT AAAAAGTGTT GGGGAAGATG ATCCTGTAAA AATGTTGCAA
    AATGTCGCAA AGAAATTTGG TTTCAGTAAT TTAAGTAGTA GGTTAGATGG AGTCAGATAT
    GAAAATGAAG AAATTAGTAT CATTGCCAAG AAAACTGGTA GTAAACCAAA GCTAAATCAG
    AAAAAATGTT CATTTTTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT
    TGTCATAAAT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA
    TGGATTGAGG CTTCCAGTTG TGCAGCTCTG GAAATGCCAA TACATTCTGA CATACTGAAA
    GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTTGTGA TAAAAGAATC TCAAAACGTG
    GATTTTGTTT GTAGTGTTCT TGTGGTGGCT GATCAGCTTC TCATATCTCG ATTGAAAGAA
    ATTTGTGAAG TAGCATTAAC TGAAAAACTT ACCCTTAAGA ATGCTGCTAT GCTACTGGAA
    TTTGCAGCAA TGTATAATGC TGAACAGTTG AAACTCTCTT GTTTACAGTT TATTGGACTG
    AATATGGCAG CTTTACTTGA AGCAAGATCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG
    GACCTTTCTG TGTTTTACCG GAAAATGATT CCAGCAATGG AGAGAAGAGT CATTACACCA
    TATCAAGATG GACCAGATAT TAGCTATTTG GATGTAGAAG ATGGAGATAT CTTTTTGAAA
    GAAGAAGTAA ATATGGAACA GAATTACTCG GAAAGTATGG TCAAGAAGAC AAAAACAAAA
    GCAAAAAAGA AGCCACGTAA ACGATCAGAT AGTTCTGGAG GTTATAATCT TTCAGATATT
    ATTCAGAGTC CACCATCTAC AGGATTGTTA AAGTCTGGTA AGACCAATTC TGTGGAGTCT
    CTTCCAGAAC TGTTGACATC AGACTCTGAA GGAAGCTATG CAGGAGTGGG TAGTCCTAGA
    GATTTACAGT CTCCTGACTT CACAACAGGA TTTCATTCAG ATAAGTCTGA GGGGAAAATT
    AAATCGTATG TTAATGGTAC ACCTCCTGCA TATTTTAGGG AAGATTTAAA ACCATGGGAA
    AAATCACCAG TACTTAAAAT AACTGCTCCA CAGCCCATTC CCAGTAACAG AATTGATACT
    ACAAGCTCTT CCAATTGGGT GTCTGGCTCT TTCAGTCCTG TCAGCCCTCC TGTCATGGAT
    CTCAGAACCA TCATGCAAAT TGAAGAAAGC AGACAAAAAT GTGGAACTAC ACCAAAGACA
    AATTTGGGAA AAATGATTTC TCATGGAGTT AAACTTTCTC AGAAGCAACG AAAAATGATT
    GCATTGACTA CCAAAGATAA TAATTCAGGA ATGAATAACA TGGAAACAGC TTTAAGTGCG
    CCTTCAAAAA TCCCCAAGCC AGCGAATGCA TGGGCATCTT CTCAACATTC AGTTTCATCC
    AAGTCATTCC GGGATTTCTT AGTAGAAGAA AAGAAATCTG TTACTGGCCA GAGATCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGG ATTGAAAATT CCCAGTCTCC AAAAGTTGTA
    AGATGTTCTG CTCATGGTTC CCCAGGACGA GAAGGCAACC ACATTTCAGA TGTACCACTT
    CCGGACAGTC CCAATCCCTG GCTGTCTTCT TCACTGACCG CTCCTTCCGT GGTAGCACCA
    GTCACTTTTG CGTCTATCGT AGAAGAAGAG CTACAGCAGG AAGCAGCTCT TATCAGAAGC
    CGAGAGAAAC CCTTGGCTTT GATTCAGATT GAGGAGCGTG CAATACAGGA TTTGTTGGTT
    TTCTATGAAG CATTTGGAAA CCCTGATGAG TTTGTCATTG TTGAAAGGAC ACCACAGGGA
    CCACTGGCAG TACCTATGTG GAATAAGCAT GGCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192969.1
    CDS1..4059
    Translation

    Target ORF information:

    RefSeq Version NM_001206040.2
    Organism Bos taurus(cattle)
    Definition Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206040.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCCT CCATCCCTGA CTGCACATCA AAGTGTCGCT CCCTGACGCA TGCTTTGGAT 
    GTCCTTTCTG TGGTAACGAA GGGGAGTGAA AACCAGATTA AGGCCTTTCT ATCCAGTCAT
    TGTCACAATG CTGCAACCAT CAGGGATGCC TTTGGTCGGA ATGCCCTCCA CCTTGTTGCC
    TCCTGTGGGA AAAAAGGAGT GTTAGATTGG CTTATTGAGA AAGGAGTGGA TCCCTCGGTG
    AAAGACAAGG AGTCAGGATG GACAGCTTTG CATAGAAGCA TTTTTTATGG GCATATTGAT
    TGTGTTTGGT CTCTACTGAA GCATGGTGTT AATCTGTATA TTCAAGATAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGATAGA CCAACTCATG TAGTATTCAA AAATACTGAT
    CCTACAGATG TCTACACTTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ATCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGAT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCACTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC
    TGTGGTCATG GTCCTGGAGG ACGATTGGGC CATGGAGATG AGCAGACATG CTTGGTCCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATAAT TGTTCTCAAG TAGCGGCTGC TAAGGATCAT
    ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACATTTG GTCTAAATAT TTTTCATCAA
    TTAGGACTCA TTCCTCCGCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC
    CTGAAAGGAA GGACAATCAT TGGAGTAGCA GCTGGCAGGT TTCATACTGT GCTGTGGACT
    AGAGAGGCTG TTTACACTAT GGGACTAAAT GGTGGACAAT TGGGTTATTT ACTAGATCCC
    AATGGAGAAA AGTGTGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGACATC
    ACTCTGTCTT TGGTTGCTGC TAGTGATGGG GCTACTGTCT GTGTTACCAC AAAGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGCTGGCTT CTAAACAACT GAACTTGAAA
    AAGGTTCTTG TATCTGGGGG GCATATGGAA TACAAAGTTG ATCCTGAACA TTTGAAAGAA
    AATGGGGGTC AGAAAATTTG CATTCTTGCA ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCTGTCA ACAGTTCTAT GAAGCAGTGT CGATGGGCTT ATCCACGCCA AGTCTTCATT
    TCTGATATTG CTTTAAGTAG AAATGAAATT CTTTTTGTCA CACAAGATGG AGAGGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAGAAAG AGTTCTGAAA AGAAAGAGAT TTTATCAAAC
    CTTCATAATT CCTCACCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATATGAAAGA
    ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT
    GGATGCAACT TTGCAGTCTT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAACT
    GTGTCCTCCT CATCCTTTTT TGAGGAGTTT GGCAAGCTGT TGAGGGAAAC GGATGAAATG
    GACAACATCC ATGATGTGAC ATTTCAAATT GGCAGTAGAC TCTTCCCTGC ACATAAATAT
    ATTTTGGCAG TGCGTTCTGA CTTTTTCCAG AAATTGTTTC TTTCGGATGG TACCGCATTA
    GACTTCACAG ATGTTTACCG GAAAGATGAA GATTCTACAG GCTGCCATCT CTTTGTGGTA
    GAGAAGGTTC ATCCTGACCT GTTTGAATAC CTTTTGCAGT TTATATACAC AGATACTTGT
    GACTTTTTAA CTCATGGCTT CAAACCAAGA CTAAATTTAA ACAGAAAACC AGAAGGATAT
    CCAAGCACTC CAAACTGTCA TTCGAATAAA ATGAATTCCC ATGAAGATAA TCAGAAGTCA
    GCATTTGAAG CTTACAAAAG TAATCAAGCT CATACAATTA GTGAAAAGCA GAAGAGTAAA
    TCCAAATCTT CCAAAAAAGT AAAAAGTGTT GGGGAAGATG ATCCTGTAAA AATGTTGCAA
    AATGTCGCAA AGAAATTTGG TTTCAGTAAT TTAAGTAGTA GGTTAGATGG AGTCAGATAT
    GAAAATGAAG AAATTAGTAT CATTGCCAAG AAAACTGGTA GTAAACCAAA GCTAAATCAG
    AAAAAATGTT CATTTTTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT
    TGTCATAAAT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA
    TGGATTGAGG CTTCCAGTTG TGCAGCTCTG GAAATGCCAA TACATTCTGA CATACTGAAA
    GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTTGTGA TAAAAGAATC TCAAAACGTG
    GATTTTGTTT GTAGTGTTCT TGTGGTGGCT GATCAGCTTC TCATATCTCG ATTGAAAGAA
    ATTTGTGAAG TAGCATTAAC TGAAAAACTT ACCCTTAAGA ATGCTGCTAT GCTACTGGAA
    TTTGCAGCAA TGTATAATGC TGAACAGTTG AAACTCTCTT GTTTACAGTT TATTGGACTG
    AATATGGCAG CTTTACTTGA AGCAAGATCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG
    GACCTTTCTG TGTTTTACCG GAAAATGATT CCAGCAATGG AGAGAAGAGT CATTACACCA
    TATCAAGATG GACCAGATAT TAGCTATTTG GATGTAGAAG ATGGAGATAT CTTTTTGAAA
    GAAGAAGTAA ATATGGAACA GAATTACTCG GAAAGTATGG TCAAGAAGAC AAAAACAAAA
    GCAAAAAAGA AGCCACGTAA ACGATCAGAT AGTTCTGGAG GTTATAATCT TTCAGATATT
    ATTCAGAGTC CACCATCTAC AGGATTGTTA AAGTCTGGTA AGACCAATTC TGTGGAGTCT
    CTTCCAGAAC TGTTGACATC AGACTCTGAA GGAAGCTATG CAGGAGTGGG TAGTCCTAGA
    GATTTACAGT CTCCTGACTT CACAACAGGA TTTCATTCAG ATAAGTCTGA GGGGAAAATT
    AAATCGTATG TTAATGGTAC ACCTCCTGCA TATTTTAGGG AAGATTTAAA ACCATGGGAA
    AAATCACCAG TACTTAAAAT AACTGCTCCA CAGCCCATTC CCAGTAACAG AATTGATACT
    ACAAGCTCTT CCAATTGGGT GTCTGGCTCT TTCAGTCCTG TCAGCCCTCC TGTCATGGAT
    CTCAGAACCA TCATGCAAAT TGAAGAAAGC AGACAAAAAT GTGGAACTAC ACCAAAGACA
    AATTTGGGAA AAATGATTTC TCATGGAGTT AAACTTTCTC AGAAGCAACG AAAAATGATT
    GCATTGACTA CCAAAGATAA TAATTCAGGA ATGAATAACA TGGAAACAGC TTTAAGTGCG
    CCTTCAAAAA TCCCCAAGCC AGCGAATGCA TGGGCATCTT CTCAACATTC AGTTTCATCC
    AAGTCATTCC GGGATTTCTT AGTAGAAGAA AAGAAATCTG TTACTGGCCA GAGATCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGG ATTGAAAATT CCCAGTCTCC AAAAGTTGTA
    AGATGTTCTG CTCATGGTTC CCCAGGACGA GAAGGCAACC ACATTTCAGA TGTACCACTT
    CCGGACAGTC CCAATCCCTG GCTGTCTTCT TCACTGACCG CTCCTTCCGT GGTAGCACCA
    GTCACTTTTG CGTCTATCGT AGAAGAAGAG CTACAGCAGG AAGCAGCTCT TATCAGAAGC
    CGAGAGAAAC CCTTGGCTTT GATTCAGATT GAGGAGCGTG CAATACAGGA TTTGTTGGTT
    TTCTATGAAG CATTTGGAAA CCCTGATGAG TTTGTCATTG TTGAAAGGAC ACCACAGGGA
    CCACTGGCAG TACCTATGTG GAATAAGCAT GGCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238923
    Clone ID Related Accession (Same CDS sequence) NM_001206040.3
    Accession Version NM_001206040.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product inhibitor of Bruton tyrosine kinase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000009.1. On May 29, 2018 this sequence version replaced NM_001206040.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571255.26421.1, SRR5721694.9096.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCCT CCATCCCTGA CTGCACATCA AAGTGTCGCT CCCTGACGCA TGCTTTGGAT 
    GTCCTTTCTG TGGTAACGAA GGGGAGTGAA AACCAGATTA AGGCCTTTCT CTCCAGTCAT
    TGTCACAATG CTGCAACCAT CAGGGATGCC TTTGGTCGGA ATGCCCTCCA CCTTGTTGCC
    TCCTGTGGGA AAAAAGGAGT GTTAGATTGG CTTATTGAGA AAGGAGTGGA TCCCTCGGTG
    AAAGACAAGG AGTCAGGATG GACAGCTTTG CATAGAAGCA TTTTTTATGG GCATATTGAT
    TGTGTTTGGT CTCTACTGAA GCATGGTGTT AATCTGTATA TTCAAGATAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGATAGA CCAACTCATG TAGTATTCAA AAATACTGAT
    CCTACAGATG TCTACACTTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ATCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGAT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCACTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC
    TGTGGTCATG GTCCTGGAGG ACGATTGGGC CATGGAGATG AGCAGACATG CTTGGTCCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATAAT TGTTCTCAAG TAGCGGCTGC TAAGGATCAT
    ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACATTTG GTCTAAATAT TTTTCATCAA
    TTAGGACTCA TTCCTCCGCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC
    CTGAAAGGAA GGACAATCAT TGGAGTAGCA GCTGGCAGGT TTCATACTGT GCTGTGGACT
    AGAGAGGCTG TTTACACTAT GGGACTAAAT GGTGGACAAT TGGGTTATTT ACTAGATCCC
    AATGGAGAAA AGTGTGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGACATC
    ACTCTGTCTT TGGTTGCTGC TAGTGATGGG GCTACTGTCT GTGTTACCAC AAAGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGCTGGCTT CTAAACAACT GAACTTGAAA
    AAGGTTCTTG TATCTGGGGG GCATATGGAA TACAAAGTTG ATCCTGAACA TTTGAAAGAA
    AATGGGGGTC AGAAAATTTG CATTCTTGCA ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCTGTCA ACAGTTCTAT GAAGCAGTGT CGATGGGCTT ATCCACGCCA AGTCTTCATT
    TCTGATATTG CTTTAAGTAG AAATGAAATT CTTTTTGTCA CACAAGATGG AGAGGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAGAAAG AGTTCTGAAA AGAAAGAGAT TTTATCAAAC
    CTTCATAATT CCTCACCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATATGAAAGA
    ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT
    GGATGCAACT TTGCAGTCTT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAACT
    GTGTCCTCCT CATCCTTTTT TGAGGAGTTT GGCAAGCTGT TGAGGGAAAC GGATGAAATG
    GACAACATCC ATGATGTGAC ATTTCAAGTT GGCAGTAGAC TCTTCCCTGC ACATAAATAT
    ATTTTGGCAG TGCGTTCTGA CTTTTTCCAG AAATTGTTTC TTTCGGATGG TACCGCATTA
    GACTTCACAG ATGTTTACCG GAAAGATGAA GATTCTACAG GCTGCCATCT CTTTGTGGTA
    GAGAAGGTTC ATCCTGACCT GTTTGAATAC CTTTTGCAGT TTATATACAC AGATACTTGT
    GACTTTTTAA CTCATGGCTT CAAACCAAGA CTAAATTTAA ACAGAAAACC AGAAGGATAT
    CCAAGCACTC CAAACTGTCA TTCGAATAAA ATGAATTCCC ATGAAGATAA TCAGAAGTCA
    GCATTTGAAG CTTACAAAAG TAATCAAGCT CATACAATTA GTGAAAAGCA GAAGAGTAAA
    TCCAAATCTT CCAAAAAAGT AAAAAGTGTT GGGGAAGATG ATCCTGTAAA AATGTTGCAA
    AATGTCGCAA AGAAATTTGG TTTCAGTAAT TTAAGTAGTA GGTTAGATGG AGTCAGATAT
    GAAAATGAAG AAATTAGTAT CATTGCCAAG AAAACTGGTA GTAAACCAAA GCTAAATCAG
    AAAAAATGTT CATTTTTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT
    TGTCATAAAT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA
    TGGATTGAGG CTTCCAGTTG TGCAGCTCTG GAAATGCCAA TACATTCTGA CATACTGAAA
    GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTTGTGA TAAAAGAATC TCAAAACGTG
    GATTTTGTTT GTAGTGTTCT TGTGGTGGCT GATCAGCTTC TCATATCTCG ATTGAAAGAA
    ATTTGTGAAG TAGCATTAAC TGAAAAACTT ACCCTTAAGA ATGCTGCTAT GCTACTGGAA
    TTTGCAGCAA TGTATAATGC TGAACAGTTG AAACTCTCTT GTTTACAGTT TATTGGACTG
    AATATGGCAG CTTTACTTGA AGCAAGATCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG
    GACCTTTCTG TGTTTTACCG GAAAATGATT CCAGCAATGG AGAGAAGAGT CATTACACCA
    TATCAAGATG GACCAGATAT TAGCTATTTG GATGTAGAAG ATGGAGATAT CTTTTTGAAA
    GAAGAAGTAA ATATGGAACA GAATTACTCG GAAAGTATGG TCAAGAAGAC AAAAACAAAA
    GCAAAAAAGA AGCCACGTAA ACGATCAGAT AGTTCTGGAG GTTATAATCT TTCAGATATT
    ATTCAGAGTC CACCATCTAC AGGATTGTTA AAGTCTGGTA AGACCAATTC TGTGGAGTCT
    CTTCCAGAAC TGTTGACATC AGACTCTGAA GGAAGCTATG CAGGAGTGGG TAGTCCTAGA
    GATTTACAGT CTCCTGACTT CACAACAGGA TTTCATTCAG ATAAGTCTGA GGGGAAAATT
    AAATCGTATG TTAATGGTAC ACCTCCTGCA TATTTTAGGG AAGATTTAAA ACCATGGGAA
    AAATCACCAG TACTTAAAAT AACTGCTCCA CAGCCCATTC CCAGTAACAG AATTGATACT
    ACAAGCTCTT CCAATTGGGT GTCTGGCTCT TTCAGTCCTG TCAGCCCTCC TGTCATGGAT
    CTCAGAACCA TCATGCAAAT TGAAGAAAGC AGACAAAAAT GTGGAACTAC ACCAAAGACA
    AATTTGGGAA AAATGATTTC TCATGGAGTT AAACTTTCTC AGAAGCAACG AAAAATGATT
    GCATTGACTA CCAAAGATAA TAATTCAGGA ATGAATAACA TGGAAACAGC TTTAAGTGCG
    CCTTCAAAAA TCCCCAAGCC AGCGAATGCA TGGGCATCTT CTCAACATTC AGTTTCATCC
    AAGTCATTCC GGGATTTCTT AGTAGAAGAA AAGAAATCTG TTACTGGCCA GAGATCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGG ATTGAAAATT CCCAGTCTCC AAAAGTTGTA
    AGATGTTCTG CTCATGGTTC CCCAGGACGA GAAGGCAACC ACATTTCAGA TGTACCACTT
    CCGGACAGTC CCAATCCCTG GCTGTCTTCT TCACTGACCG CTCCTTCCGT GGTAGCACCA
    GTCACTTTTG CGTCTATCGT AGAAGAAGAG CTACAGCAGG AAGCAGCTCT TATCAGAAGC
    CGAGAGAAAC CCTTGGCTTT GATTCAGATT GAGGAGCGTG CAATACAGGA TTTGTTGGTT
    TTCTATGAAG CATTTGGAAA CCCTGATGAG TTTGTCATTG TTGAAAGGAC ACCACAGGGA
    CCACTGGCAG TACCTATGTG GAATAAGCAT GGCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192969.2
    CDS1..4059
    Translation

    Target ORF information:

    RefSeq Version NM_001206040.3
    Organism Bos taurus(cattle)
    Definition Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206040.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCCT CCATCCCTGA CTGCACATCA AAGTGTCGCT CCCTGACGCA TGCTTTGGAT 
    GTCCTTTCTG TGGTAACGAA GGGGAGTGAA AACCAGATTA AGGCCTTTCT CTCCAGTCAT
    TGTCACAATG CTGCAACCAT CAGGGATGCC TTTGGTCGGA ATGCCCTCCA CCTTGTTGCC
    TCCTGTGGGA AAAAAGGAGT GTTAGATTGG CTTATTGAGA AAGGAGTGGA TCCCTCGGTG
    AAAGACAAGG AGTCAGGATG GACAGCTTTG CATAGAAGCA TTTTTTATGG GCATATTGAT
    TGTGTTTGGT CTCTACTGAA GCATGGTGTT AATCTGTATA TTCAAGATAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGATAGA CCAACTCATG TAGTATTCAA AAATACTGAT
    CCTACAGATG TCTACACTTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ATCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGAT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCACTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC
    TGTGGTCATG GTCCTGGAGG ACGATTGGGC CATGGAGATG AGCAGACATG CTTGGTCCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATAAT TGTTCTCAAG TAGCGGCTGC TAAGGATCAT
    ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACATTTG GTCTAAATAT TTTTCATCAA
    TTAGGACTCA TTCCTCCGCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC
    CTGAAAGGAA GGACAATCAT TGGAGTAGCA GCTGGCAGGT TTCATACTGT GCTGTGGACT
    AGAGAGGCTG TTTACACTAT GGGACTAAAT GGTGGACAAT TGGGTTATTT ACTAGATCCC
    AATGGAGAAA AGTGTGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGACATC
    ACTCTGTCTT TGGTTGCTGC TAGTGATGGG GCTACTGTCT GTGTTACCAC AAAGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGCTGGCTT CTAAACAACT GAACTTGAAA
    AAGGTTCTTG TATCTGGGGG GCATATGGAA TACAAAGTTG ATCCTGAACA TTTGAAAGAA
    AATGGGGGTC AGAAAATTTG CATTCTTGCA ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCTGTCA ACAGTTCTAT GAAGCAGTGT CGATGGGCTT ATCCACGCCA AGTCTTCATT
    TCTGATATTG CTTTAAGTAG AAATGAAATT CTTTTTGTCA CACAAGATGG AGAGGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAGAAAG AGTTCTGAAA AGAAAGAGAT TTTATCAAAC
    CTTCATAATT CCTCACCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATATGAAAGA
    ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT
    GGATGCAACT TTGCAGTCTT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAACT
    GTGTCCTCCT CATCCTTTTT TGAGGAGTTT GGCAAGCTGT TGAGGGAAAC GGATGAAATG
    GACAACATCC ATGATGTGAC ATTTCAAGTT GGCAGTAGAC TCTTCCCTGC ACATAAATAT
    ATTTTGGCAG TGCGTTCTGA CTTTTTCCAG AAATTGTTTC TTTCGGATGG TACCGCATTA
    GACTTCACAG ATGTTTACCG GAAAGATGAA GATTCTACAG GCTGCCATCT CTTTGTGGTA
    GAGAAGGTTC ATCCTGACCT GTTTGAATAC CTTTTGCAGT TTATATACAC AGATACTTGT
    GACTTTTTAA CTCATGGCTT CAAACCAAGA CTAAATTTAA ACAGAAAACC AGAAGGATAT
    CCAAGCACTC CAAACTGTCA TTCGAATAAA ATGAATTCCC ATGAAGATAA TCAGAAGTCA
    GCATTTGAAG CTTACAAAAG TAATCAAGCT CATACAATTA GTGAAAAGCA GAAGAGTAAA
    TCCAAATCTT CCAAAAAAGT AAAAAGTGTT GGGGAAGATG ATCCTGTAAA AATGTTGCAA
    AATGTCGCAA AGAAATTTGG TTTCAGTAAT TTAAGTAGTA GGTTAGATGG AGTCAGATAT
    GAAAATGAAG AAATTAGTAT CATTGCCAAG AAAACTGGTA GTAAACCAAA GCTAAATCAG
    AAAAAATGTT CATTTTTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT
    TGTCATAAAT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA
    TGGATTGAGG CTTCCAGTTG TGCAGCTCTG GAAATGCCAA TACATTCTGA CATACTGAAA
    GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTTGTGA TAAAAGAATC TCAAAACGTG
    GATTTTGTTT GTAGTGTTCT TGTGGTGGCT GATCAGCTTC TCATATCTCG ATTGAAAGAA
    ATTTGTGAAG TAGCATTAAC TGAAAAACTT ACCCTTAAGA ATGCTGCTAT GCTACTGGAA
    TTTGCAGCAA TGTATAATGC TGAACAGTTG AAACTCTCTT GTTTACAGTT TATTGGACTG
    AATATGGCAG CTTTACTTGA AGCAAGATCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG
    GACCTTTCTG TGTTTTACCG GAAAATGATT CCAGCAATGG AGAGAAGAGT CATTACACCA
    TATCAAGATG GACCAGATAT TAGCTATTTG GATGTAGAAG ATGGAGATAT CTTTTTGAAA
    GAAGAAGTAA ATATGGAACA GAATTACTCG GAAAGTATGG TCAAGAAGAC AAAAACAAAA
    GCAAAAAAGA AGCCACGTAA ACGATCAGAT AGTTCTGGAG GTTATAATCT TTCAGATATT
    ATTCAGAGTC CACCATCTAC AGGATTGTTA AAGTCTGGTA AGACCAATTC TGTGGAGTCT
    CTTCCAGAAC TGTTGACATC AGACTCTGAA GGAAGCTATG CAGGAGTGGG TAGTCCTAGA
    GATTTACAGT CTCCTGACTT CACAACAGGA TTTCATTCAG ATAAGTCTGA GGGGAAAATT
    AAATCGTATG TTAATGGTAC ACCTCCTGCA TATTTTAGGG AAGATTTAAA ACCATGGGAA
    AAATCACCAG TACTTAAAAT AACTGCTCCA CAGCCCATTC CCAGTAACAG AATTGATACT
    ACAAGCTCTT CCAATTGGGT GTCTGGCTCT TTCAGTCCTG TCAGCCCTCC TGTCATGGAT
    CTCAGAACCA TCATGCAAAT TGAAGAAAGC AGACAAAAAT GTGGAACTAC ACCAAAGACA
    AATTTGGGAA AAATGATTTC TCATGGAGTT AAACTTTCTC AGAAGCAACG AAAAATGATT
    GCATTGACTA CCAAAGATAA TAATTCAGGA ATGAATAACA TGGAAACAGC TTTAAGTGCG
    CCTTCAAAAA TCCCCAAGCC AGCGAATGCA TGGGCATCTT CTCAACATTC AGTTTCATCC
    AAGTCATTCC GGGATTTCTT AGTAGAAGAA AAGAAATCTG TTACTGGCCA GAGATCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGG ATTGAAAATT CCCAGTCTCC AAAAGTTGTA
    AGATGTTCTG CTCATGGTTC CCCAGGACGA GAAGGCAACC ACATTTCAGA TGTACCACTT
    CCGGACAGTC CCAATCCCTG GCTGTCTTCT TCACTGACCG CTCCTTCCGT GGTAGCACCA
    GTCACTTTTG CGTCTATCGT AGAAGAAGAG CTACAGCAGG AAGCAGCTCT TATCAGAAGC
    CGAGAGAAAC CCTTGGCTTT GATTCAGATT GAGGAGCGTG CAATACAGGA TTTGTTGGTT
    TTCTATGAAG CATTTGGAAA CCCTGATGAG TTTGTCATTG TTGAAAGGAC ACCACAGGGA
    CCACTGGCAG TACCTATGTG GAATAAGCAT GGCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237881
    Clone ID Related Accession (Same CDS sequence) XM_005210695.4
    Accession Version XM_005210695.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4248bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inhibitor of Bruton tyrosine kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210695.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCAAAAT ACATTGTACA AATCTATATA GTTTTATACT GTTGCCAGGA GGATTTAAAT 
    TATAACCCTA AGCAGGCCGT TTATTCTCTA TCAGTCTTTC TGAATAGCAG CTTTGTGGCC
    TGGCTTTTTC ATTTTTTAAA ATTATCTGAC TATTCTGAAG AAGGAAGCTG CATAAAAACA
    AATAGGATGA ATTCCTCCAT CCCTGACTGC ACATCAAAGT GTCGCTCCCT GACGCATGCT
    TTGGATGTCC TTTCTGTGGT AACGAAGGGG AGTGAAAACC AGATTAAGGC CTTTCTCTCC
    AGTCATTGTC ACAATGCTGC AACCATCAGG GATGCCTTTG GTCGGAATGC CCTCCACCTT
    GTTGCCTCCT GTGGGAAAAA AGGAGTGTTA GATTGGCTTA TTGAGAAAGG AGTGGATCCC
    TCGGTGAAAG ACAAGGAGTC AGGATGGACA GCTTTGCATA GAAGCATTTT TTATGGGCAT
    ATTGATTGTG TTTGGTCTCT ACTGAAGCAT GGTGTTAATC TGTATATTCA AGATAAAGAA
    GGCTTGTCAG CTTTGGATCT TGTAATGAAG GATAGACCAA CTCATGTAGT ATTCAAAAAT
    ACTGATCCTA CAGATGTCTA CACTTGGGGA GATAATACAA ATTTCACCCT GGGTCATGGA
    AGCCAGAATA GCAAACATCA TCCAGAGTTG GTGGATCTGT TCTCCAGGAG TGGGATTTAT
    ATCAAGCAGG TGGTGCTTTG TAAATTTCAC TCAGTGTTTC TGTCTCAGAA AGGGCAGGTT
    TACACCTGTG GTCATGGTCC TGGAGGACGA TTGGGCCATG GAGATGAGCA GACATGCTTG
    GTCCCTAGGC TTGTGGAAGG ACTGAGTGGT CATAATTGTT CTCAAGTAGC GGCTGCTAAG
    GATCATACTG TTGTATTAAC TGAAGATGGA TGTGTTTATA CATTTGGTCT AAATATTTTT
    CATCAATTAG GACTCATTCC TCCGCCTTCC AGTTGTAATG TACCCAGACA GATGCAGGCA
    AAGTACCTGA AAGGAAGGAC AATCATTGGA GTAGCAGCTG GCAGGTTTCA TACTGTGCTG
    TGGACTAGAG AGGCTGTTTA CACTATGGGA CTAAATGGTG GACAATTGGG TTATTTACTA
    GATCCCAATG GAGAAAAGTG TGTAACTGCT CCTCGTCAAG TCTCTGCCCT TCATCATAAG
    GACATCACTC TGTCTTTGGT TGCTGCTAGT GATGGGGCTA CTGTCTGTGT TACCACAAAG
    GGAGATATTT ACTTACTTGC AGACTATCAG TGCAAGAAGC TGGCTTCTAA ACAACTGAAC
    TTGAAAAAGG TTCTTGTATC TGGGGGGCAT ATGGAATACA AAGTTGATCC TGAACATTTG
    AAAGAAAATG GGGGTCAGAA AATTTGCATT CTTGCAATGG ATGGAGCTGG AAGGGTATTT
    TGCTGGAGAT CTGTCAACAG TTCTATGAAG CAGTGTCGAT GGGCTTATCC ACGCCAAGTC
    TTCATTTCTG ATATTGCTTT AAGTAGAAAT GAAATTCTTT TTGTCACACA AGATGGAGAG
    GGATTTAGAG GGAAATGGTT TGAAGAAAAA AGAAAGAGTT CTGAAAAGAA AGCAGAGATT
    TTATCAAACC TTCATAATTC CTCACCAGAT GTGTCTTGTG TCTCTGATAT AAATAGTGTA
    TATGAAAGAA TTCGACTTGA GAAACTTACT TTTGCCCATA GAGCTGTTAG TGTCAGCACA
    GATCCAAGTG GATGCAACTT TGCAGTCTTG CAGTCAGATC CTAAAACAAG CCTTTATGAA
    ATTCCAACTG TGTCCTCCTC ATCCTTTTTT GAGGAGTTTG GCAAGCTGTT GAGGGAAACG
    GATGAAATGG ACAACATCCA TGATGTGACA TTTCAAGTTG GCAGTAGACT CTTCCCTGCA
    CATAAATATA TTTTGGCAGT GCGTTCTGAC TTTTTCCAGA AATTGTTTCT TTCGGATGGT
    ACCGCATTAG ACTTCACAGA TGTTTACCGG AAAGATGAAG ATTCTACAGG CTGCCATCTC
    TTTGTGGTAG AGAAGGTTCA TCCTGACCTG TTTGAATACC TTTTGCAGTT TATATACACA
    GATACTTGTG ACTTTTTAAC TCATGGCTTC AAACCAAGAC TAAATTTAAA CAGAAAACCA
    GAAGGATATC CAAGCACTCC AAACTGTCAT TCGAATAAAA TGAATTCCCA TGAAGATAAT
    CAGAAGTCAG CATTTGAAGC TTACAAAAGT AATCAAGCTC ATACAATTAG TGAAAAGCAG
    AAGAGTAAAT CCAAATCTTC CAAAAAAGTA AAAAGTGTTG GGGAAGATGA TCCTGTAAAA
    ATGTTGCAAA ATGTCGCAAA GAAATTTGGT TTCAGTAATT TAAGTAGTAG GTTAGATGGA
    GTCAGATATG AAAATGAAGA AATTAGTATC ATTGCCAAGA AAACTGGTAG TAAACCAAAG
    CTAAATCAGA AAAAATGTTC ATTTTTATGT GATGTGACCA TGAAATCAGT GGATGGAAAG
    GAATTTCCTT GTCATAAATG TGTTCTTTGT GCTAGACTTG AATATTTTCA TAGTATGCTG
    AGTAGCTCAT GGATTGAGGC TTCCAGTTGT GCAGCTCTGG AAATGCCAAT ACATTCTGAC
    ATACTGAAAG TTATTTTGGA CTACCTCTAT ACTGATGAAG CTGTTGTGAT AAAAGAATCT
    CAAAACGTGG ATTTTGTTTG TAGTGTTCTT GTGGTGGCTG ATCAGCTTCT CATATCTCGA
    TTGAAAGAAA TTTGTGAAGT AGCATTAACT GAAAAACTTA CCCTTAAGAA TGCTGCTATG
    CTACTGGAAT TTGCAGCAAT GTATAATGCT GAACAGTTGA AACTCTCTTG TTTACAGTTT
    ATTGGACTGA ATATGGCAGC TTTACTTGAA GCAAGATCTC TTGATGTTTT AAGTGATGGT
    GTTTTGAAGG ACCTTTCTGT GTTTTACCGG AAAATGATTC CAGCAATGGA GAGAAGAGTC
    ATTACACCAT ATCAAGATGG ACCAGATATT AGCTATTTGG ATGTAGAAGA TGGAGATATC
    TTTTTGAAAG AAGAAGTAAA TATGGAACAG AATTACTCGG AAAGTATGGT CAAGAAGACA
    AAAACAAAAG CAAAAAAGAA GCCACGTAAA CGATCAGATA GTTCTGGAGG TTATAATCTT
    TCAGATATTA TTCAGAGTCC ACCATCTACA GGATTGTTAA AGTCTGGTAA GACCAATTCT
    GTGGAGTCTC TTCCAGAACT GTTGACATCA GACTCTGAAG GAAGCTATGC AGGAGTGGGT
    AGTCCTAGAG ATTTACAGTC TCCTGACTTC ACAACAGGAT TTCATTCAGA TAAGTCTGAG
    GGGAAAATTA AATCGTATGT TAATGGTACA CCTCCTGCAT ATTTTAGGGA AGATTTAAAA
    CCATGGGAAA AATCACCAGT ACTTAAAATA ACTGCTCCAC AGCCCATTCC CAGTAACAGA
    ATTGATACTA CAAGCTCTTC CAATTGGGTG TCTGGCTCTT TCAGTCCTGT CAGCCCTCCT
    GTCATGGATC TCAGAACCAT CATGCAAATT GAAGAAAGCA GACAAAAATG TGGAACTACA
    CCAAAGACAA ATTTGGGAAA AATGATTTCT CATGGAGTTA AACTTTCTCA GAAGCAACGA
    AAAATGATTG CATTGACTAC CAAAGATAAT AATTCAGGAA TGAATAACAT GGAAACAGCT
    TTAAGTGCGC CTTCAAAAAT CCCCAAGCCA GCGAATGCAT GGGCATCTTC TCAACATTCA
    GTTTCATCCA AGTCATTCCG GGATTTCTTA GTAGAAGAAA AGAAATCTGT TACTGGCCAG
    AGATCAGGAG ATCATGTCAA AAAAGTTTGT TTTAAAGGGA TTGAAAATTC CCAGTCTCCA
    AAAGTTGTAA GATGTTCTGC TCATGGTTCC CCAGGACGAG AAGGCAACCA CATTTCAGAT
    GTACCACTTC CGGACAGTCC CAATCCCTGG CTGTCTTCTT CACTGACCGC TCCTTCCGTG
    GTAGCACCAG TCACTTTTGC GTCTATCGTA GAAGAAGAGC TACAGCAGGA AGCAGCTCTT
    ATCAGAAGCC GAGAGAAACC CTTGGCTTTG ATTCAGATTG AGGAGCGTGC AATACAGGAT
    TTGTTGGTTT TCTATGAAGC ATTTGGAAAC CCTGATGAGT TTGTCATTGT TGAAAGGACA
    CCACAGGGAC CACTGGCAGT ACCTATGTGG AATAAGCATG GCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210752.2
    CDS395..4642
    Translation

    Target ORF information:

    RefSeq Version XM_005210695.4
    Organism Bos taurus(cattle)
    Definition Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210695.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCAAAAT ACATTGTACA AATCTATATA GTTTTATACT GTTGCCAGGA GGATTTAAAT 
    TATAACCCTA AGCAGGCCGT TTATTCTCTA TCAGTCTTTC TGAATAGCAG CTTTGTGGCC
    TGGCTTTTTC ATTTTTTAAA ATTATCTGAC TATTCTGAAG AAGGAAGCTG CATAAAAACA
    AATAGGATGA ATTCCTCCAT CCCTGACTGC ACATCAAAGT GTCGCTCCCT GACGCATGCT
    TTGGATGTCC TTTCTGTGGT AACGAAGGGG AGTGAAAACC AGATTAAGGC CTTTCTCTCC
    AGTCATTGTC ACAATGCTGC AACCATCAGG GATGCCTTTG GTCGGAATGC CCTCCACCTT
    GTTGCCTCCT GTGGGAAAAA AGGAGTGTTA GATTGGCTTA TTGAGAAAGG AGTGGATCCC
    TCGGTGAAAG ACAAGGAGTC AGGATGGACA GCTTTGCATA GAAGCATTTT TTATGGGCAT
    ATTGATTGTG TTTGGTCTCT ACTGAAGCAT GGTGTTAATC TGTATATTCA AGATAAAGAA
    GGCTTGTCAG CTTTGGATCT TGTAATGAAG GATAGACCAA CTCATGTAGT ATTCAAAAAT
    ACTGATCCTA CAGATGTCTA CACTTGGGGA GATAATACAA ATTTCACCCT GGGTCATGGA
    AGCCAGAATA GCAAACATCA TCCAGAGTTG GTGGATCTGT TCTCCAGGAG TGGGATTTAT
    ATCAAGCAGG TGGTGCTTTG TAAATTTCAC TCAGTGTTTC TGTCTCAGAA AGGGCAGGTT
    TACACCTGTG GTCATGGTCC TGGAGGACGA TTGGGCCATG GAGATGAGCA GACATGCTTG
    GTCCCTAGGC TTGTGGAAGG ACTGAGTGGT CATAATTGTT CTCAAGTAGC GGCTGCTAAG
    GATCATACTG TTGTATTAAC TGAAGATGGA TGTGTTTATA CATTTGGTCT AAATATTTTT
    CATCAATTAG GACTCATTCC TCCGCCTTCC AGTTGTAATG TACCCAGACA GATGCAGGCA
    AAGTACCTGA AAGGAAGGAC AATCATTGGA GTAGCAGCTG GCAGGTTTCA TACTGTGCTG
    TGGACTAGAG AGGCTGTTTA CACTATGGGA CTAAATGGTG GACAATTGGG TTATTTACTA
    GATCCCAATG GAGAAAAGTG TGTAACTGCT CCTCGTCAAG TCTCTGCCCT TCATCATAAG
    GACATCACTC TGTCTTTGGT TGCTGCTAGT GATGGGGCTA CTGTCTGTGT TACCACAAAG
    GGAGATATTT ACTTACTTGC AGACTATCAG TGCAAGAAGC TGGCTTCTAA ACAACTGAAC
    TTGAAAAAGG TTCTTGTATC TGGGGGGCAT ATGGAATACA AAGTTGATCC TGAACATTTG
    AAAGAAAATG GGGGTCAGAA AATTTGCATT CTTGCAATGG ATGGAGCTGG AAGGGTATTT
    TGCTGGAGAT CTGTCAACAG TTCTATGAAG CAGTGTCGAT GGGCTTATCC ACGCCAAGTC
    TTCATTTCTG ATATTGCTTT AAGTAGAAAT GAAATTCTTT TTGTCACACA AGATGGAGAG
    GGATTTAGAG GGAAATGGTT TGAAGAAAAA AGAAAGAGTT CTGAAAAGAA AGCAGAGATT
    TTATCAAACC TTCATAATTC CTCACCAGAT GTGTCTTGTG TCTCTGATAT AAATAGTGTA
    TATGAAAGAA TTCGACTTGA GAAACTTACT TTTGCCCATA GAGCTGTTAG TGTCAGCACA
    GATCCAAGTG GATGCAACTT TGCAGTCTTG CAGTCAGATC CTAAAACAAG CCTTTATGAA
    ATTCCAACTG TGTCCTCCTC ATCCTTTTTT GAGGAGTTTG GCAAGCTGTT GAGGGAAACG
    GATGAAATGG ACAACATCCA TGATGTGACA TTTCAAGTTG GCAGTAGACT CTTCCCTGCA
    CATAAATATA TTTTGGCAGT GCGTTCTGAC TTTTTCCAGA AATTGTTTCT TTCGGATGGT
    ACCGCATTAG ACTTCACAGA TGTTTACCGG AAAGATGAAG ATTCTACAGG CTGCCATCTC
    TTTGTGGTAG AGAAGGTTCA TCCTGACCTG TTTGAATACC TTTTGCAGTT TATATACACA
    GATACTTGTG ACTTTTTAAC TCATGGCTTC AAACCAAGAC TAAATTTAAA CAGAAAACCA
    GAAGGATATC CAAGCACTCC AAACTGTCAT TCGAATAAAA TGAATTCCCA TGAAGATAAT
    CAGAAGTCAG CATTTGAAGC TTACAAAAGT AATCAAGCTC ATACAATTAG TGAAAAGCAG
    AAGAGTAAAT CCAAATCTTC CAAAAAAGTA AAAAGTGTTG GGGAAGATGA TCCTGTAAAA
    ATGTTGCAAA ATGTCGCAAA GAAATTTGGT TTCAGTAATT TAAGTAGTAG GTTAGATGGA
    GTCAGATATG AAAATGAAGA AATTAGTATC ATTGCCAAGA AAACTGGTAG TAAACCAAAG
    CTAAATCAGA AAAAATGTTC ATTTTTATGT GATGTGACCA TGAAATCAGT GGATGGAAAG
    GAATTTCCTT GTCATAAATG TGTTCTTTGT GCTAGACTTG AATATTTTCA TAGTATGCTG
    AGTAGCTCAT GGATTGAGGC TTCCAGTTGT GCAGCTCTGG AAATGCCAAT ACATTCTGAC
    ATACTGAAAG TTATTTTGGA CTACCTCTAT ACTGATGAAG CTGTTGTGAT AAAAGAATCT
    CAAAACGTGG ATTTTGTTTG TAGTGTTCTT GTGGTGGCTG ATCAGCTTCT CATATCTCGA
    TTGAAAGAAA TTTGTGAAGT AGCATTAACT GAAAAACTTA CCCTTAAGAA TGCTGCTATG
    CTACTGGAAT TTGCAGCAAT GTATAATGCT GAACAGTTGA AACTCTCTTG TTTACAGTTT
    ATTGGACTGA ATATGGCAGC TTTACTTGAA GCAAGATCTC TTGATGTTTT AAGTGATGGT
    GTTTTGAAGG ACCTTTCTGT GTTTTACCGG AAAATGATTC CAGCAATGGA GAGAAGAGTC
    ATTACACCAT ATCAAGATGG ACCAGATATT AGCTATTTGG ATGTAGAAGA TGGAGATATC
    TTTTTGAAAG AAGAAGTAAA TATGGAACAG AATTACTCGG AAAGTATGGT CAAGAAGACA
    AAAACAAAAG CAAAAAAGAA GCCACGTAAA CGATCAGATA GTTCTGGAGG TTATAATCTT
    TCAGATATTA TTCAGAGTCC ACCATCTACA GGATTGTTAA AGTCTGGTAA GACCAATTCT
    GTGGAGTCTC TTCCAGAACT GTTGACATCA GACTCTGAAG GAAGCTATGC AGGAGTGGGT
    AGTCCTAGAG ATTTACAGTC TCCTGACTTC ACAACAGGAT TTCATTCAGA TAAGTCTGAG
    GGGAAAATTA AATCGTATGT TAATGGTACA CCTCCTGCAT ATTTTAGGGA AGATTTAAAA
    CCATGGGAAA AATCACCAGT ACTTAAAATA ACTGCTCCAC AGCCCATTCC CAGTAACAGA
    ATTGATACTA CAAGCTCTTC CAATTGGGTG TCTGGCTCTT TCAGTCCTGT CAGCCCTCCT
    GTCATGGATC TCAGAACCAT CATGCAAATT GAAGAAAGCA GACAAAAATG TGGAACTACA
    CCAAAGACAA ATTTGGGAAA AATGATTTCT CATGGAGTTA AACTTTCTCA GAAGCAACGA
    AAAATGATTG CATTGACTAC CAAAGATAAT AATTCAGGAA TGAATAACAT GGAAACAGCT
    TTAAGTGCGC CTTCAAAAAT CCCCAAGCCA GCGAATGCAT GGGCATCTTC TCAACATTCA
    GTTTCATCCA AGTCATTCCG GGATTTCTTA GTAGAAGAAA AGAAATCTGT TACTGGCCAG
    AGATCAGGAG ATCATGTCAA AAAAGTTTGT TTTAAAGGGA TTGAAAATTC CCAGTCTCCA
    AAAGTTGTAA GATGTTCTGC TCATGGTTCC CCAGGACGAG AAGGCAACCA CATTTCAGAT
    GTACCACTTC CGGACAGTCC CAATCCCTGG CTGTCTTCTT CACTGACCGC TCCTTCCGTG
    GTAGCACCAG TCACTTTTGC GTCTATCGTA GAAGAAGAGC TACAGCAGGA AGCAGCTCTT
    ATCAGAAGCC GAGAGAAACC CTTGGCTTTG ATTCAGATTG AGGAGCGTGC AATACAGGAT
    TTGTTGGTTT TCTATGAAGC ATTTGGAAAC CCTGATGAGT TTGTCATTGT TGAAAGGACA
    CCACAGGGAC CACTGGCAGT ACCTATGTGG AATAAGCATG GCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237882
    Clone ID Related Accession (Same CDS sequence) XM_005210696.4
    Accession Version XM_005210696.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4245bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product inhibitor of Bruton tyrosine kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210696.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCAAAAT ACATTGTACA AATCTATATA GTTTTATACT GTTGCCAGGA GGATTTAAAT 
    TATAACCCTA AGCAGGCCGT TTATTCTCTA TCAGTCTTTC TGAATAGCAG CTTTGTGGCC
    TGGCTTTTTC ATTTTTTAAA ATTATCTGAC TATTCTGAAG AAGGAAGCTG CATAAAAACA
    AATAGGATGA ATTCCTCCAT CCCTGACTGC ACATCAAAGT GTCGCTCCCT GACGCATGCT
    TTGGATGTCC TTTCTGTGGT AACGAAGGGG AGTGAAAACC AGATTAAGGC CTTTCTCTCC
    AGTCATTGTC ACAATGCTGC AACCATCAGG GATGCCTTTG GTCGGAATGC CCTCCACCTT
    GTTGCCTCCT GTGGGAAAAA AGGAGTGTTA GATTGGCTTA TTGAGAAAGG AGTGGATCCC
    TCGGTGAAAG ACAAGGAGTC AGGATGGACA GCTTTGCATA GAAGCATTTT TTATGGGCAT
    ATTGATTGTG TTTGGTCTCT ACTGAAGCAT GGTGTTAATC TGTATATTCA AGATAAAGAA
    GGCTTGTCAG CTTTGGATCT TGTAATGAAG GATAGACCAA CTCATGTAGT ATTCAAAAAT
    ACTGATCCTA CAGATGTCTA CACTTGGGGA GATAATACAA ATTTCACCCT GGGTCATGGA
    AGCCAGAATA GCAAACATCA TCCAGAGTTG GTGGATCTGT TCTCCAGGAG TGGGATTTAT
    ATCAAGCAGG TGGTGCTTTG TAAATTTCAC TCAGTGTTTC TGTCTCAGAA AGGGCAGGTT
    TACACCTGTG GTCATGGTCC TGGAGGACGA TTGGGCCATG GAGATGAGCA GACATGCTTG
    GTCCCTAGGC TTGTGGAAGG ACTGAGTGGT CATAATTGTT CTCAAGTAGC GGCTGCTAAG
    GATCATACTG TTGTATTAAC TGAAGATGGA TGTGTTTATA CATTTGGTCT AAATATTTTT
    CATCAATTAG GACTCATTCC TCCGCCTTCC AGTTGTAATG TACCCAGACA GATGCAGGCA
    AAGTACCTGA AAGGAAGGAC AATCATTGGA GTAGCAGCTG GCAGGTTTCA TACTGTGCTG
    TGGACTAGAG AGGCTGTTTA CACTATGGGA CTAAATGGTG GACAATTGGG TTATTTACTA
    GATCCCAATG GAGAAAAGTG TGTAACTGCT CCTCGTCAAG TCTCTGCCCT TCATCATAAG
    GACATCACTC TGTCTTTGGT TGCTGCTAGT GATGGGGCTA CTGTCTGTGT TACCACAAAG
    GGAGATATTT ACTTACTTGC AGACTATCAG TGCAAGAAGC TGGCTTCTAA ACAACTGAAC
    TTGAAAAAGG TTCTTGTATC TGGGGGGCAT ATGGAATACA AAGTTGATCC TGAACATTTG
    AAAGAAAATG GGGGTCAGAA AATTTGCATT CTTGCAATGG ATGGAGCTGG AAGGGTATTT
    TGCTGGAGAT CTGTCAACAG TTCTATGAAG CAGTGTCGAT GGGCTTATCC ACGCCAAGTC
    TTCATTTCTG ATATTGCTTT AAGTAGAAAT GAAATTCTTT TTGTCACACA AGATGGAGAG
    GGATTTAGAG GGAAATGGTT TGAAGAAAAA AGAAAGAGTT CTGAAAAGAA AGAGATTTTA
    TCAAACCTTC ATAATTCCTC ACCAGATGTG TCTTGTGTCT CTGATATAAA TAGTGTATAT
    GAAAGAATTC GACTTGAGAA ACTTACTTTT GCCCATAGAG CTGTTAGTGT CAGCACAGAT
    CCAAGTGGAT GCAACTTTGC AGTCTTGCAG TCAGATCCTA AAACAAGCCT TTATGAAATT
    CCAACTGTGT CCTCCTCATC CTTTTTTGAG GAGTTTGGCA AGCTGTTGAG GGAAACGGAT
    GAAATGGACA ACATCCATGA TGTGACATTT CAAGTTGGCA GTAGACTCTT CCCTGCACAT
    AAATATATTT TGGCAGTGCG TTCTGACTTT TTCCAGAAAT TGTTTCTTTC GGATGGTACC
    GCATTAGACT TCACAGATGT TTACCGGAAA GATGAAGATT CTACAGGCTG CCATCTCTTT
    GTGGTAGAGA AGGTTCATCC TGACCTGTTT GAATACCTTT TGCAGTTTAT ATACACAGAT
    ACTTGTGACT TTTTAACTCA TGGCTTCAAA CCAAGACTAA ATTTAAACAG AAAACCAGAA
    GGATATCCAA GCACTCCAAA CTGTCATTCG AATAAAATGA ATTCCCATGA AGATAATCAG
    AAGTCAGCAT TTGAAGCTTA CAAAAGTAAT CAAGCTCATA CAATTAGTGA AAAGCAGAAG
    AGTAAATCCA AATCTTCCAA AAAAGTAAAA AGTGTTGGGG AAGATGATCC TGTAAAAATG
    TTGCAAAATG TCGCAAAGAA ATTTGGTTTC AGTAATTTAA GTAGTAGGTT AGATGGAGTC
    AGATATGAAA ATGAAGAAAT TAGTATCATT GCCAAGAAAA CTGGTAGTAA ACCAAAGCTA
    AATCAGAAAA AATGTTCATT TTTATGTGAT GTGACCATGA AATCAGTGGA TGGAAAGGAA
    TTTCCTTGTC ATAAATGTGT TCTTTGTGCT AGACTTGAAT ATTTTCATAG TATGCTGAGT
    AGCTCATGGA TTGAGGCTTC CAGTTGTGCA GCTCTGGAAA TGCCAATACA TTCTGACATA
    CTGAAAGTTA TTTTGGACTA CCTCTATACT GATGAAGCTG TTGTGATAAA AGAATCTCAA
    AACGTGGATT TTGTTTGTAG TGTTCTTGTG GTGGCTGATC AGCTTCTCAT ATCTCGATTG
    AAAGAAATTT GTGAAGTAGC ATTAACTGAA AAACTTACCC TTAAGAATGC TGCTATGCTA
    CTGGAATTTG CAGCAATGTA TAATGCTGAA CAGTTGAAAC TCTCTTGTTT ACAGTTTATT
    GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGATCTCTTG ATGTTTTAAG TGATGGTGTT
    TTGAAGGACC TTTCTGTGTT TTACCGGAAA ATGATTCCAG CAATGGAGAG AAGAGTCATT
    ACACCATATC AAGATGGACC AGATATTAGC TATTTGGATG TAGAAGATGG AGATATCTTT
    TTGAAAGAAG AAGTAAATAT GGAACAGAAT TACTCGGAAA GTATGGTCAA GAAGACAAAA
    ACAAAAGCAA AAAAGAAGCC ACGTAAACGA TCAGATAGTT CTGGAGGTTA TAATCTTTCA
    GATATTATTC AGAGTCCACC ATCTACAGGA TTGTTAAAGT CTGGTAAGAC CAATTCTGTG
    GAGTCTCTTC CAGAACTGTT GACATCAGAC TCTGAAGGAA GCTATGCAGG AGTGGGTAGT
    CCTAGAGATT TACAGTCTCC TGACTTCACA ACAGGATTTC ATTCAGATAA GTCTGAGGGG
    AAAATTAAAT CGTATGTTAA TGGTACACCT CCTGCATATT TTAGGGAAGA TTTAAAACCA
    TGGGAAAAAT CACCAGTACT TAAAATAACT GCTCCACAGC CCATTCCCAG TAACAGAATT
    GATACTACAA GCTCTTCCAA TTGGGTGTCT GGCTCTTTCA GTCCTGTCAG CCCTCCTGTC
    ATGGATCTCA GAACCATCAT GCAAATTGAA GAAAGCAGAC AAAAATGTGG AACTACACCA
    AAGACAAATT TGGGAAAAAT GATTTCTCAT GGAGTTAAAC TTTCTCAGAA GCAACGAAAA
    ATGATTGCAT TGACTACCAA AGATAATAAT TCAGGAATGA ATAACATGGA AACAGCTTTA
    AGTGCGCCTT CAAAAATCCC CAAGCCAGCG AATGCATGGG CATCTTCTCA ACATTCAGTT
    TCATCCAAGT CATTCCGGGA TTTCTTAGTA GAAGAAAAGA AATCTGTTAC TGGCCAGAGA
    TCAGGAGATC ATGTCAAAAA AGTTTGTTTT AAAGGGATTG AAAATTCCCA GTCTCCAAAA
    GTTGTAAGAT GTTCTGCTCA TGGTTCCCCA GGACGAGAAG GCAACCACAT TTCAGATGTA
    CCACTTCCGG ACAGTCCCAA TCCCTGGCTG TCTTCTTCAC TGACCGCTCC TTCCGTGGTA
    GCACCAGTCA CTTTTGCGTC TATCGTAGAA GAAGAGCTAC AGCAGGAAGC AGCTCTTATC
    AGAAGCCGAG AGAAACCCTT GGCTTTGATT CAGATTGAGG AGCGTGCAAT ACAGGATTTG
    TTGGTTTTCT ATGAAGCATT TGGAAACCCT GATGAGTTTG TCATTGTTGA AAGGACACCA
    CAGGGACCAC TGGCAGTACC TATGTGGAAT AAGCATGGCT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210753.2
    CDS395..4639
    Translation

    Target ORF information:

    RefSeq Version XM_005210696.4
    Organism Bos taurus(cattle)
    Definition Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210696.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCAAAAT ACATTGTACA AATCTATATA GTTTTATACT GTTGCCAGGA GGATTTAAAT 
    TATAACCCTA AGCAGGCCGT TTATTCTCTA TCAGTCTTTC TGAATAGCAG CTTTGTGGCC
    TGGCTTTTTC ATTTTTTAAA ATTATCTGAC TATTCTGAAG AAGGAAGCTG CATAAAAACA
    AATAGGATGA ATTCCTCCAT CCCTGACTGC ACATCAAAGT GTCGCTCCCT GACGCATGCT
    TTGGATGTCC TTTCTGTGGT AACGAAGGGG AGTGAAAACC AGATTAAGGC CTTTCTCTCC
    AGTCATTGTC ACAATGCTGC AACCATCAGG GATGCCTTTG GTCGGAATGC CCTCCACCTT
    GTTGCCTCCT GTGGGAAAAA AGGAGTGTTA GATTGGCTTA TTGAGAAAGG AGTGGATCCC
    TCGGTGAAAG ACAAGGAGTC AGGATGGACA GCTTTGCATA GAAGCATTTT TTATGGGCAT
    ATTGATTGTG TTTGGTCTCT ACTGAAGCAT GGTGTTAATC TGTATATTCA AGATAAAGAA
    GGCTTGTCAG CTTTGGATCT TGTAATGAAG GATAGACCAA CTCATGTAGT ATTCAAAAAT
    ACTGATCCTA CAGATGTCTA CACTTGGGGA GATAATACAA ATTTCACCCT GGGTCATGGA
    AGCCAGAATA GCAAACATCA TCCAGAGTTG GTGGATCTGT TCTCCAGGAG TGGGATTTAT
    ATCAAGCAGG TGGTGCTTTG TAAATTTCAC TCAGTGTTTC TGTCTCAGAA AGGGCAGGTT
    TACACCTGTG GTCATGGTCC TGGAGGACGA TTGGGCCATG GAGATGAGCA GACATGCTTG
    GTCCCTAGGC TTGTGGAAGG ACTGAGTGGT CATAATTGTT CTCAAGTAGC GGCTGCTAAG
    GATCATACTG TTGTATTAAC TGAAGATGGA TGTGTTTATA CATTTGGTCT AAATATTTTT
    CATCAATTAG GACTCATTCC TCCGCCTTCC AGTTGTAATG TACCCAGACA GATGCAGGCA
    AAGTACCTGA AAGGAAGGAC AATCATTGGA GTAGCAGCTG GCAGGTTTCA TACTGTGCTG
    TGGACTAGAG AGGCTGTTTA CACTATGGGA CTAAATGGTG GACAATTGGG TTATTTACTA
    GATCCCAATG GAGAAAAGTG TGTAACTGCT CCTCGTCAAG TCTCTGCCCT TCATCATAAG
    GACATCACTC TGTCTTTGGT TGCTGCTAGT GATGGGGCTA CTGTCTGTGT TACCACAAAG
    GGAGATATTT ACTTACTTGC AGACTATCAG TGCAAGAAGC TGGCTTCTAA ACAACTGAAC
    TTGAAAAAGG TTCTTGTATC TGGGGGGCAT ATGGAATACA AAGTTGATCC TGAACATTTG
    AAAGAAAATG GGGGTCAGAA AATTTGCATT CTTGCAATGG ATGGAGCTGG AAGGGTATTT
    TGCTGGAGAT CTGTCAACAG TTCTATGAAG CAGTGTCGAT GGGCTTATCC ACGCCAAGTC
    TTCATTTCTG ATATTGCTTT AAGTAGAAAT GAAATTCTTT TTGTCACACA AGATGGAGAG
    GGATTTAGAG GGAAATGGTT TGAAGAAAAA AGAAAGAGTT CTGAAAAGAA AGAGATTTTA
    TCAAACCTTC ATAATTCCTC ACCAGATGTG TCTTGTGTCT CTGATATAAA TAGTGTATAT
    GAAAGAATTC GACTTGAGAA ACTTACTTTT GCCCATAGAG CTGTTAGTGT CAGCACAGAT
    CCAAGTGGAT GCAACTTTGC AGTCTTGCAG TCAGATCCTA AAACAAGCCT TTATGAAATT
    CCAACTGTGT CCTCCTCATC CTTTTTTGAG GAGTTTGGCA AGCTGTTGAG GGAAACGGAT
    GAAATGGACA ACATCCATGA TGTGACATTT CAAGTTGGCA GTAGACTCTT CCCTGCACAT
    AAATATATTT TGGCAGTGCG TTCTGACTTT TTCCAGAAAT TGTTTCTTTC GGATGGTACC
    GCATTAGACT TCACAGATGT TTACCGGAAA GATGAAGATT CTACAGGCTG CCATCTCTTT
    GTGGTAGAGA AGGTTCATCC TGACCTGTTT GAATACCTTT TGCAGTTTAT ATACACAGAT
    ACTTGTGACT TTTTAACTCA TGGCTTCAAA CCAAGACTAA ATTTAAACAG AAAACCAGAA
    GGATATCCAA GCACTCCAAA CTGTCATTCG AATAAAATGA ATTCCCATGA AGATAATCAG
    AAGTCAGCAT TTGAAGCTTA CAAAAGTAAT CAAGCTCATA CAATTAGTGA AAAGCAGAAG
    AGTAAATCCA AATCTTCCAA AAAAGTAAAA AGTGTTGGGG AAGATGATCC TGTAAAAATG
    TTGCAAAATG TCGCAAAGAA ATTTGGTTTC AGTAATTTAA GTAGTAGGTT AGATGGAGTC
    AGATATGAAA ATGAAGAAAT TAGTATCATT GCCAAGAAAA CTGGTAGTAA ACCAAAGCTA
    AATCAGAAAA AATGTTCATT TTTATGTGAT GTGACCATGA AATCAGTGGA TGGAAAGGAA
    TTTCCTTGTC ATAAATGTGT TCTTTGTGCT AGACTTGAAT ATTTTCATAG TATGCTGAGT
    AGCTCATGGA TTGAGGCTTC CAGTTGTGCA GCTCTGGAAA TGCCAATACA TTCTGACATA
    CTGAAAGTTA TTTTGGACTA CCTCTATACT GATGAAGCTG TTGTGATAAA AGAATCTCAA
    AACGTGGATT TTGTTTGTAG TGTTCTTGTG GTGGCTGATC AGCTTCTCAT ATCTCGATTG
    AAAGAAATTT GTGAAGTAGC ATTAACTGAA AAACTTACCC TTAAGAATGC TGCTATGCTA
    CTGGAATTTG CAGCAATGTA TAATGCTGAA CAGTTGAAAC TCTCTTGTTT ACAGTTTATT
    GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGATCTCTTG ATGTTTTAAG TGATGGTGTT
    TTGAAGGACC TTTCTGTGTT TTACCGGAAA ATGATTCCAG CAATGGAGAG AAGAGTCATT
    ACACCATATC AAGATGGACC AGATATTAGC TATTTGGATG TAGAAGATGG AGATATCTTT
    TTGAAAGAAG AAGTAAATAT GGAACAGAAT TACTCGGAAA GTATGGTCAA GAAGACAAAA
    ACAAAAGCAA AAAAGAAGCC ACGTAAACGA TCAGATAGTT CTGGAGGTTA TAATCTTTCA
    GATATTATTC AGAGTCCACC ATCTACAGGA TTGTTAAAGT CTGGTAAGAC CAATTCTGTG
    GAGTCTCTTC CAGAACTGTT GACATCAGAC TCTGAAGGAA GCTATGCAGG AGTGGGTAGT
    CCTAGAGATT TACAGTCTCC TGACTTCACA ACAGGATTTC ATTCAGATAA GTCTGAGGGG
    AAAATTAAAT CGTATGTTAA TGGTACACCT CCTGCATATT TTAGGGAAGA TTTAAAACCA
    TGGGAAAAAT CACCAGTACT TAAAATAACT GCTCCACAGC CCATTCCCAG TAACAGAATT
    GATACTACAA GCTCTTCCAA TTGGGTGTCT GGCTCTTTCA GTCCTGTCAG CCCTCCTGTC
    ATGGATCTCA GAACCATCAT GCAAATTGAA GAAAGCAGAC AAAAATGTGG AACTACACCA
    AAGACAAATT TGGGAAAAAT GATTTCTCAT GGAGTTAAAC TTTCTCAGAA GCAACGAAAA
    ATGATTGCAT TGACTACCAA AGATAATAAT TCAGGAATGA ATAACATGGA AACAGCTTTA
    AGTGCGCCTT CAAAAATCCC CAAGCCAGCG AATGCATGGG CATCTTCTCA ACATTCAGTT
    TCATCCAAGT CATTCCGGGA TTTCTTAGTA GAAGAAAAGA AATCTGTTAC TGGCCAGAGA
    TCAGGAGATC ATGTCAAAAA AGTTTGTTTT AAAGGGATTG AAAATTCCCA GTCTCCAAAA
    GTTGTAAGAT GTTCTGCTCA TGGTTCCCCA GGACGAGAAG GCAACCACAT TTCAGATGTA
    CCACTTCCGG ACAGTCCCAA TCCCTGGCTG TCTTCTTCAC TGACCGCTCC TTCCGTGGTA
    GCACCAGTCA CTTTTGCGTC TATCGTAGAA GAAGAGCTAC AGCAGGAAGC AGCTCTTATC
    AGAAGCCGAG AGAAACCCTT GGCTTTGATT CAGATTGAGG AGCGTGCAAT ACAGGATTTG
    TTGGTTTTCT ATGAAGCATT TGGAAACCCT GATGAGTTTG TCATTGTTGA AAGGACACCA
    CAGGGACCAC TGGCAGTACC TATGTGGAAT AAGCATGGCT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54896
    Clone ID Related Accession (Same CDS sequence) XM_010808382.2
    Accession Version XM_010808382.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inhibitor of Bruton tyrosine kinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104175.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCCAAAAT ACATTGTACA AATCTATATA GTTTTATACT GTTGCCAGGA GGATTTAAAT 
    TATAACCCTA AGCAGGCCGT TTATTCTCTA TCAGTCTTTC TGAATAGCAG CTTTGTGGCC
    TGGCTTTTTC ATTTTTTAAA ATTATCTGAC TATTCTGAAG AAGGAAGCTG CATAAAAACA
    AATAGGATGA ATTCCTCCAT CCCTGACTGC ACATCAAAGT GTCGCTCCCT GACGCATGCT
    TTGGATGTCC TTTCTGTGGT AACGAAGGGG AGTGAAAACC AGATTAAGGC CTTTCTATCC
    AGTCATTGTC ACAATGCTGC AACCATCAGG GATGCCTTTG GTCGGAATGC CCTCCACCTT
    GTTGCCTCCT GTGGGAAAAA AGGAGTGTTA GATTGGCTTA TTGAGAAAGG AGTGGATCCC
    TCGGTGAAAG ACAAGGAGTC AGGATGGACA GCTTTGCATA GAAGCATTTT TTATGGGCAT
    ATTGATTGTG TTTGGTCTCT ACTGAAGCAT GGTGTTAATC TGTATATTCA AGATAAAGAA
    GGCTTGTCAG CTTTGGATCT TGTAATGAAG GATAGACCAA CTCATGTAGT ATTCAAAAAT
    ACTGATCCTA CAGATGTCTA CACTTGGGGA GATAATACAA ATTTCACCCT GGGTCATGGA
    AGCCAGAATA GCAAACATCA TCCAGAGTTG GTGGATCTGT TCTCCAGGAG TGGGATTTAT
    ATCAAGCAGG TGGTGCTTTG TAAATTTCAC TCAGTGTTTC TGTCTCAGAA AGGGCAGGTT
    TACACCTGTG GTCATGGTCC TGGAGGACGA TTGGGCCATG GAGATGAGCA GACATGCTTG
    GTCCCTAGGC TTGTGGAAGG ACTGAGTGGT CATAATTGTT CTCAAGTAGC GGCTGCTAAG
    GATCATACTG TTGTATTAAC TGAAGATGGA TGTGTTTATA CATTTGGTCT AAATATTTTT
    CATCAATTAG GACTCATTCC TCCGCCTTCC AGTTGTAATG TACCCAGACA GATGCAGGCA
    AAGTACCTGA AAGGAAGGAC AATCATTGGA GTAGCAGCTG GCAGGTTTCA TACTGTGCTG
    TGGACTAGAG AGGCTGTTTA CACTATGGGA CTAAATGGTG GACAATTGGG TTATTTACTA
    GATCCCAATG GAGAAAAGTG TGTAACTGCT CCTCGTCAAG TCTCTGCCCT TCATCATAAG
    GACATCACTC TGTCTTTGGT TGCTGCTAGT GATGGGGCTA CTGTCTGTGT TACCACAAAG
    GGAGATATTT ACTTACTTGC AGACTATCAG TGCAAGAAGC TGGCTTCTAA ACAACTGAAC
    TTGAAAAAGG TTCTTGTATC TGGGGGGCAT ATGGAATACA AAGTTGATCC TGAACATTTG
    AAAGAAAATG GGGGTCAGAA AATTTGCATT CTTGCAATGG ATGGAGCTGG AAGGGTATTT
    TGCTGGAGAT CTGTCAACAG TTCTATGAAG CAGTGTCGAT GGGCTTATCC ACGCCAAGTC
    TTCATTTCTG ATATTGCTTT AAGTAGAAAT GAAATTCTTT TTGTCACACA AGATGGAGAG
    GGATTTAGAG GGAAATGGTT TGAAGAAAAA AGAAAGAGTT CTGAAAAGAA AGCAGAGATT
    TTATCAAACC TTCATAATTC CTCACCAGAT GTGTCTTGTG TCTCTGATAT AAATAGTGTA
    TATGAAAGAA TTCGACTTGA GAAACTTACT TTTGCCCATA GAGCTGTTAG TGTCAGCACA
    GATCCAAGTG GATGCAACTT TGCAGTCTTG CAGTCAGATC CTAAAACAAG CCTTTATGAA
    ATTCCAACTG TGTCCTCCTC ATCCTTTTTT GAGGAGTTTG GCAAGCTGTT GAGGGAAACG
    GATGAAATGG ACAACATCCA TGATGTGACA TTTCAAATTG GCAGTAGACT CTTCCCTGCA
    CATAAATATA TTTTGGCAGT GCGTTCTGAC TTTTTCCAGA AATTGTTTCT TTCGGATGGT
    ACCGCATTAG ACTTCACAGA TGTTTACCGG AAAGATGAAG ATTCTACAGG CTGCCATCTC
    TTTGTGGTAG AGAAGGTTCA TCCTGACCTG TTTGAATACC TTTTGCAGTT TATATACACA
    GATACTTGTG ACTTTTTAAC TCATGGCTTC AAACCAAGAC TAAATTTAAA CAGAAAACCA
    GAAGGATATC CAAGCACTCC AAACTGTCAT TCGAATAAAA TGAATTCCCA TGAAGATAAT
    CAGAAGTCAG CATTTGAAGC TTACAAAAGT AATCAAGCTC ATACAATTAG TGAAAAGCAG
    AAGAGTAAAT CCAAATCTTC CAAAAAAGTA AAAAGTGTTG GGGAAGATGA TCCTGTAAAA
    ATGTTGCAAA ATGTCGCAAA GAAATTTGGT TTCAGTAATT TAAGTAGTAG GTTAGATGGA
    GTCAGATATG AAAATGAAGA AATTAGTATC ATTGCCAAGA AAACTGGTAG TAAACCAAAG
    CTAAATCAGA AAAAATGTTC ATTTTTATGT GATGTGACCA TGAAATCAGT GGATGGAAAG
    GAATTTCCTT GTCATAAATG TGTTCTTTGT GCTAGACTTG AATATTTTCA TAGTATGCTG
    AGTAGCTCAT GGATTGAGGC TTCCAGTTGT GCAGCTCTGG AAATGCCAAT ACATTCTGAC
    ATACTGAAAG TTATTTTGGA CTACCTCTAT ACTGATGAAG CTGTTGTGAT AAAAGAATCT
    CAAAACGTGG ATTTTGTTTG TAGTGTTCTT GTGGTGGCTG ATCAGCTTCT CATATCTCGA
    TTGAAAGAAA TTTGTGAAGT AGCATTAACT GAAAAACTTA CCCTTAAGAA TGCTGCTATG
    CTACTGGAAT TTGCAGCAAT GTATAATGCT GAACAGTTGA AACTCTCTTG TTTACAGTTT
    ATTGGACTGA ATATGGCAGC TTTACTTGAA GCAAGATCTC TTGATGTTTT AAGTGATGGT
    GTTTTGAAGG ACCTTTCTGT GTTTTACCGG AAAATGATTC CAGCAATGGA GAGAAGAGTC
    ATTACACCAT ATCAAGATGG ACCAGATATT AGCTATTTGG ATGTAGAAGA TGGAGATATC
    TTTTTGAAAG AAGAAGTAAA TATGGAACAG AATTACTCGG AAAGTATGGT CAAGAAGACA
    AAAACAAAAG CAAAAAAGAA GCCACGTAAA CGATCAGATA GTTCTGGAGG TTATAATCTT
    TCAGATATTA TTCAGAGTCC ACCATCTACA GGATTGTTAA AGTCTGGTAA GACCAATTCT
    GTGGAGTCTC TTCCAGAACT GTTGACATCA GACTCTGAAG GAAGCTATGC AGGAGTGGGT
    AGTCCTAGAG ATTTACAGTC TCCTGACTTC ACAACAGGAT TTCATTCAGA TAAGTCTGAG
    GGGAAAATTA AATCGTATGT TAATGGTACA CCTCCTGCAT ATTTTAGGGA AGATTTAAAA
    CCATGGGAAA AATCACCAGT ACTTAAAATA ACTGCTCCAC AGCCCATTCC CAGTAACAGA
    ATTGATACTA CAAGCTCTTC CAATTGGGTG TCTGGCTCTT TCAGTCCTGT CAGCCCTCCT
    GTCATGGATC TCAGAACCAT CATGCAAATT GAAGAAAGCA GACAAAAATG TGGAACTACA
    CCAAAGACAA ATTTGGGAAA AATGATTTCT CATGGAGTTA AACTTTCTCA GAAGCAACGA
    AAAATGATTG CATTGACTAC CAAAGATAAT AATTCAGGAA TGAATAACAT GGAAACAGCT
    TTAAGTGCGC CTTCAAAAAT CCCCAAGCCA GCGAATGCAT GGGCATCTTC TCAACATTCA
    GTTTCATCCA AGTCATTCCG GGATTTCTTA GTAGAAGAAA AGAAATCTGT TACTGGCCAG
    AGATCAGGAG ATCATGTCAA AAAAGTTTGT TTTAAAGGGA TTGAAAATTC CCAGTCTCCA
    AAAGTTGTAA GAGAGAAAAG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806684.1
    CDS354..4277
    Translation

    Target ORF information:

    RefSeq Version XM_010808382.2
    Organism Bos taurus(cattle)
    Definition Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808382.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCCAAAAT ACATTGTACA AATCTATATA GTTTTATACT GTTGCCAGGA GGATTTAAAT 
    TATAACCCTA AGCAGGCCGT TTATTCTCTA TCAGTCTTTC TGAATAGCAG CTTTGTGGCC
    TGGCTTTTTC ATTTTTTAAA ATTATCTGAC TATTCTGAAG AAGGAAGCTG CATAAAAACA
    AATAGGATGA ATTCCTCCAT CCCTGACTGC ACATCAAAGT GTCGCTCCCT GACGCATGCT
    TTGGATGTCC TTTCTGTGGT AACGAAGGGG AGTGAAAACC AGATTAAGGC CTTTCTATCC
    AGTCATTGTC ACAATGCTGC AACCATCAGG GATGCCTTTG GTCGGAATGC CCTCCACCTT
    GTTGCCTCCT GTGGGAAAAA AGGAGTGTTA GATTGGCTTA TTGAGAAAGG AGTGGATCCC
    TCGGTGAAAG ACAAGGAGTC AGGATGGACA GCTTTGCATA GAAGCATTTT TTATGGGCAT
    ATTGATTGTG TTTGGTCTCT ACTGAAGCAT GGTGTTAATC TGTATATTCA AGATAAAGAA
    GGCTTGTCAG CTTTGGATCT TGTAATGAAG GATAGACCAA CTCATGTAGT ATTCAAAAAT
    ACTGATCCTA CAGATGTCTA CACTTGGGGA GATAATACAA ATTTCACCCT GGGTCATGGA
    AGCCAGAATA GCAAACATCA TCCAGAGTTG GTGGATCTGT TCTCCAGGAG TGGGATTTAT
    ATCAAGCAGG TGGTGCTTTG TAAATTTCAC TCAGTGTTTC TGTCTCAGAA AGGGCAGGTT
    TACACCTGTG GTCATGGTCC TGGAGGACGA TTGGGCCATG GAGATGAGCA GACATGCTTG
    GTCCCTAGGC TTGTGGAAGG ACTGAGTGGT CATAATTGTT CTCAAGTAGC GGCTGCTAAG
    GATCATACTG TTGTATTAAC TGAAGATGGA TGTGTTTATA CATTTGGTCT AAATATTTTT
    CATCAATTAG GACTCATTCC TCCGCCTTCC AGTTGTAATG TACCCAGACA GATGCAGGCA
    AAGTACCTGA AAGGAAGGAC AATCATTGGA GTAGCAGCTG GCAGGTTTCA TACTGTGCTG
    TGGACTAGAG AGGCTGTTTA CACTATGGGA CTAAATGGTG GACAATTGGG TTATTTACTA
    GATCCCAATG GAGAAAAGTG TGTAACTGCT CCTCGTCAAG TCTCTGCCCT TCATCATAAG
    GACATCACTC TGTCTTTGGT TGCTGCTAGT GATGGGGCTA CTGTCTGTGT TACCACAAAG
    GGAGATATTT ACTTACTTGC AGACTATCAG TGCAAGAAGC TGGCTTCTAA ACAACTGAAC
    TTGAAAAAGG TTCTTGTATC TGGGGGGCAT ATGGAATACA AAGTTGATCC TGAACATTTG
    AAAGAAAATG GGGGTCAGAA AATTTGCATT CTTGCAATGG ATGGAGCTGG AAGGGTATTT
    TGCTGGAGAT CTGTCAACAG TTCTATGAAG CAGTGTCGAT GGGCTTATCC ACGCCAAGTC
    TTCATTTCTG ATATTGCTTT AAGTAGAAAT GAAATTCTTT TTGTCACACA AGATGGAGAG
    GGATTTAGAG GGAAATGGTT TGAAGAAAAA AGAAAGAGTT CTGAAAAGAA AGCAGAGATT
    TTATCAAACC TTCATAATTC CTCACCAGAT GTGTCTTGTG TCTCTGATAT AAATAGTGTA
    TATGAAAGAA TTCGACTTGA GAAACTTACT TTTGCCCATA GAGCTGTTAG TGTCAGCACA
    GATCCAAGTG GATGCAACTT TGCAGTCTTG CAGTCAGATC CTAAAACAAG CCTTTATGAA
    ATTCCAACTG TGTCCTCCTC ATCCTTTTTT GAGGAGTTTG GCAAGCTGTT GAGGGAAACG
    GATGAAATGG ACAACATCCA TGATGTGACA TTTCAAATTG GCAGTAGACT CTTCCCTGCA
    CATAAATATA TTTTGGCAGT GCGTTCTGAC TTTTTCCAGA AATTGTTTCT TTCGGATGGT
    ACCGCATTAG ACTTCACAGA TGTTTACCGG AAAGATGAAG ATTCTACAGG CTGCCATCTC
    TTTGTGGTAG AGAAGGTTCA TCCTGACCTG TTTGAATACC TTTTGCAGTT TATATACACA
    GATACTTGTG ACTTTTTAAC TCATGGCTTC AAACCAAGAC TAAATTTAAA CAGAAAACCA
    GAAGGATATC CAAGCACTCC AAACTGTCAT TCGAATAAAA TGAATTCCCA TGAAGATAAT
    CAGAAGTCAG CATTTGAAGC TTACAAAAGT AATCAAGCTC ATACAATTAG TGAAAAGCAG
    AAGAGTAAAT CCAAATCTTC CAAAAAAGTA AAAAGTGTTG GGGAAGATGA TCCTGTAAAA
    ATGTTGCAAA ATGTCGCAAA GAAATTTGGT TTCAGTAATT TAAGTAGTAG GTTAGATGGA
    GTCAGATATG AAAATGAAGA AATTAGTATC ATTGCCAAGA AAACTGGTAG TAAACCAAAG
    CTAAATCAGA AAAAATGTTC ATTTTTATGT GATGTGACCA TGAAATCAGT GGATGGAAAG
    GAATTTCCTT GTCATAAATG TGTTCTTTGT GCTAGACTTG AATATTTTCA TAGTATGCTG
    AGTAGCTCAT GGATTGAGGC TTCCAGTTGT GCAGCTCTGG AAATGCCAAT ACATTCTGAC
    ATACTGAAAG TTATTTTGGA CTACCTCTAT ACTGATGAAG CTGTTGTGAT AAAAGAATCT
    CAAAACGTGG ATTTTGTTTG TAGTGTTCTT GTGGTGGCTG ATCAGCTTCT CATATCTCGA
    TTGAAAGAAA TTTGTGAAGT AGCATTAACT GAAAAACTTA CCCTTAAGAA TGCTGCTATG
    CTACTGGAAT TTGCAGCAAT GTATAATGCT GAACAGTTGA AACTCTCTTG TTTACAGTTT
    ATTGGACTGA ATATGGCAGC TTTACTTGAA GCAAGATCTC TTGATGTTTT AAGTGATGGT
    GTTTTGAAGG ACCTTTCTGT GTTTTACCGG AAAATGATTC CAGCAATGGA GAGAAGAGTC
    ATTACACCAT ATCAAGATGG ACCAGATATT AGCTATTTGG ATGTAGAAGA TGGAGATATC
    TTTTTGAAAG AAGAAGTAAA TATGGAACAG AATTACTCGG AAAGTATGGT CAAGAAGACA
    AAAACAAAAG CAAAAAAGAA GCCACGTAAA CGATCAGATA GTTCTGGAGG TTATAATCTT
    TCAGATATTA TTCAGAGTCC ACCATCTACA GGATTGTTAA AGTCTGGTAA GACCAATTCT
    GTGGAGTCTC TTCCAGAACT GTTGACATCA GACTCTGAAG GAAGCTATGC AGGAGTGGGT
    AGTCCTAGAG ATTTACAGTC TCCTGACTTC ACAACAGGAT TTCATTCAGA TAAGTCTGAG
    GGGAAAATTA AATCGTATGT TAATGGTACA CCTCCTGCAT ATTTTAGGGA AGATTTAAAA
    CCATGGGAAA AATCACCAGT ACTTAAAATA ACTGCTCCAC AGCCCATTCC CAGTAACAGA
    ATTGATACTA CAAGCTCTTC CAATTGGGTG TCTGGCTCTT TCAGTCCTGT CAGCCCTCCT
    GTCATGGATC TCAGAACCAT CATGCAAATT GAAGAAAGCA GACAAAAATG TGGAACTACA
    CCAAAGACAA ATTTGGGAAA AATGATTTCT CATGGAGTTA AACTTTCTCA GAAGCAACGA
    AAAATGATTG CATTGACTAC CAAAGATAAT AATTCAGGAA TGAATAACAT GGAAACAGCT
    TTAAGTGCGC CTTCAAAAAT CCCCAAGCCA GCGAATGCAT GGGCATCTTC TCAACATTCA
    GTTTCATCCA AGTCATTCCG GGATTTCTTA GTAGAAGAAA AGAAATCTGT TACTGGCCAG
    AGATCAGGAG ATCATGTCAA AAAAGTTTGT TTTAAAGGGA TTGAAAATTC CCAGTCTCCA
    AAAGTTGTAA GAGAGAAAAG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54897
    Clone ID Related Accession (Same CDS sequence) XM_010808383.2
    Accession Version XM_010808383.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3915bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inhibitor of Bruton tyrosine kinase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104175.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808383.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCCAAAAT ACATTGTACA AATCTATATA GTTTTATACT GTTGCCAGGA GGATTTAAAT 
    TATAACCCTA AGCAGGCCGT TTATTCTCTA TCAGTCTTTC TGAATAGCAG CTTTGTGGCC
    TGGCTTTTTC ATTTTTTAAA ATTATCTGAC TATTCTGAAG AAGGAAGCTG CATAAAAACA
    AATAGGATGA ATTCCTCCAT CCCTGACTGC ACATCAAAGT GTCGCTCCCT GACGCATGCT
    TTGGATGTCC TTTCTGTGGT AACGAAGGGG AGTGAAAACC AGATTAAGGC CTTTCTATCC
    AGTCATTGTC ACAATGCTGC AACCATCAGG GATGCCTTTG GTCGGAATGC CCTCCACCTT
    GTTGCCTCCT GTGGGAAAAA AGGAGTGTTA GATTGGCTTA TTGAGAAAGG AGTGGATCCC
    TCGGTGAAAG ACAAGGAGTC AGGATGGACA GCTTTGCATA GAAGCATTTT TTATGGGCAT
    ATTGATTGTG TTTGGTCTCT ACTGAAGCAT GGTGTTAATC TGTATATTCA AGATAAAGAA
    GGCTTGTCAG CTTTGGATCT TGTAATGAAG GATAGACCAA CTCATGTAGT ATTCAAAAAT
    ACTGATCCTA CAGATGTCTA CACTTGGGGA GATAATACAA ATTTCACCCT GGGTCATGGA
    AGCCAGAATA GCAAACATCA TCCAGAGTTG GTGGATCTGT TCTCCAGGAG TGGGATTTAT
    ATCAAGCAGG TGGTGCTTTG TAAATTTCAC TCAGTGTTTC TGTCTCAGAA AGGGCAGGTT
    TACACCTGTG GTCATGGTCC TGGAGGACGA TTGGGCCATG GAGATGAGCA GACATGCTTG
    GTCCCTAGGC TTGTGGAAGG ACTGAGTGGT CATAATTGTT CTCAAGTAGC GGCTGCTAAG
    GATCATACTG TTGTATTAAC TGAAGATGGA TGTGTTTATA CATTTGGTCT AAATATTTTT
    CATCAATTAG GACTCATTCC TCCGCCTTCC AGTTGTAATG TACCCAGACA GATGCAGGCA
    AAGTACCTGA AAGGAAGGAC AATCATTGGA GTAGCAGCTG GCAGGTTTCA TACTGTGCTG
    TGGACTAGAG AGGCTGTTTA CACTATGGGA CTAAATGGTG GACAATTGGG TTATTTACTA
    GATCCCAATG GAGAAAAGTG TGTAACTGCT CCTCGTCAAG TCTCTGCCCT TCATCATAAG
    GACATCACTC TGTCTTTGGT TGCTGCTAGT GATGGGGCTA CTGTCTGTGT TACCACAAAG
    GGAGATATTT ACTTACTTGC AGACTATCAG TGCAAGAAGC TGGCTTCTAA ACAACTGAAC
    TTGAAAAAGG TTCTTGTATC TGGGGGGCAT ATGGAATACA AAGTTGATCC TGAACATTTG
    AAAGAAAATG GGGGTCAGAA AATTTGCATT CTTGCAATGG ATGGAGCTGG AAGGGTATTT
    TGCTGGAGAT CTGTCAACAG TTCTATGAAG CAGTGTCGAT GGGCTTATCC ACGCCAAGTC
    TTCATTTCTG ATATTGCTTT AAGTAGAAAT GAAATTCTTT TTGTCACACA AGATGGAGAG
    GGATTTAGAG GGAAATGGTT TGAAGAAAAA AGAAAGAGTT CTGAAAAGAA AGCAGAGATT
    TTATCAAACC TTCATAATTC CTCACCAGAT GTGTCTTGTG TCTCTGATAT AAATAGTGTA
    TATGAAAGAA TTCGACTTGA GAAACTTACT TTTGCCCATA GAGCTGTTAG TGTCAGCACA
    GATCCAAGTG GATGCAACTT TGCAGTCTTG CAGTCAGATC CTAAAACAAG CCTTTATGAA
    ATTCCAACTG TGTCCTCCTC ATCCTTTTTT GAGGAGTTTG GCAAGCTGTT GAGGGAAACG
    GATGAAATGG ACAACATCCA TGATGTGACA TTTCAAATTG GCAGTAGACT CTTCCCTGCA
    CATAAATATA TTTTGGCAGT GCGTTCTGAC TTTTTCCAGA AATTGTTTCT TTCGGATGGT
    ACCGCATTAG ACTTCACAGA TGTTTACCGG AAAGATGAAG ATTCTACAGG CTGCCATCTC
    TTTGTGGTAG AGAAGGTTCA TCCTGACCTG TTTGAATACC TTTTGCAGTT TATATACACA
    GATACTTGTG ACTTTTTAAC TCATGGCTTC AAACCAAGAC TAAATTTAAA CAGAAAACCA
    GAAGGATATC CAAGCACTCC AAACTGTCAT TCGAATAAAA TGAATTCCCA TGAAGATAAT
    CAGAAGTCAG CATTTGAAGC TTACAAAAGT AATCAAGCTC ATACAATTAG TGAAAAGCAG
    AAGAGTAAAT CCAAATCTTC CAAAAAAGTA AAAAGTGTTG GGGAAGATGA TCCTGTAAAA
    ATGTTGCAAA ATGTCGCAAA GAAATTTGGT TTCAGTAATT TAAGTAGTAG GTTAGATGGA
    GTCAGATATG AAAATGAAGA AATTAGTATC ATTGCCAAGA AAACTGGTAG TAAACCAAAG
    CTAAATCAGA AAAAATGTTC ATTTTTATGT GATGTGACCA TGAAATCAGT GGATGGAAAG
    GAATTTCCTT GTCATAAATG TGTTCTTTGT GCTAGACTTG AATATTTTCA TAGTATGCTG
    AGTAGCTCAT GGATTGAGGC TTCCAGTTGT GCAGCTCTGG AAATGCCAAT ACATTCTGAC
    ATACTGAAAG TTATTTTGGA CTACCTCTAT ACTGATGAAG CTGTTGTGAT AAAAGAATCT
    CAAAACGTGG ATTTTGTTTG TAGTGTTCTT GTGGTGGCTG ATCAGCTTCT CATATCTCGA
    TTGAAAGAAA TTTGTGAAGT AGCATTAACT GAAAAACTTA CCCTTAAGAA TGCTGCTATG
    CTACTGGAAT TTGCAGCAAT GTATAATGCT GAACAGTTGA AACTCTCTTG TTTACAGTTT
    ATTGGACTGA ATATGGCAGC TTTACTTGAA GCAAGATCTC TTGATGTTTT AAGTGATGGT
    GTTTTGAAGG ACCTTTCTGT GTTTTACCGG AAAATGATTC CAGCAATGGA GAGAAGAGTC
    ATTACACCAT ATCAAGATGG ACCAGATATT AGCTATTTGG ATGTAGAAGA TGGAGATATC
    TTTTTGAAAG AAGAAGTAAA TATGGAACAG AATTACTCGG AAAGTATGGT CAAGAAGACA
    AAAACAAAAG CAAAAAAGAA GCCACGTAAA CGATCAGATA GTTCTGGAGG TTATAATCTT
    TCAGATATTA TTCAGAGTCC ACCATCTACA GGATTGTTAA AGTCTGGTAA GACCAATTCT
    GTGGAGTCTC TTCCAGAACT GTTGACATCA GACTCTGAAG GAAGCTATGC AGGAGTGGGT
    AGTCCTAGAG ATTTACAGTC TCCTGACTTC ACAACAGGAT TTCATTCAGA TAAGTCTGAG
    GGGAAAATTA AATCGTATGT TAATGGTACA CCTCCTGCAT ATTTTAGGGA AGATTTAAAA
    CCATGGGAAA AATCACCAGT ACTTAAAATA ACTGCTCCAC AGCCCATTCC CAGTAACAGA
    ATTGATACTA CAAGCTCTTC CAATTGGGTG TCTGGCTCTT TCAGTCCTGT CAGCCCTCCT
    GTCATGGATC TCAGAACCAT CATGCAAATT GAAGAAAGCA GACAAAAATG TGGAACTACA
    CCAAAGACAA ATTTGGGAAA AATGATTTCT CATGGAGTTA AACTTTCTCA GAAGCAACGA
    AAAATGATTG CATTGACTAC CAAAGATAAT AATTCAGGAA TGAATAACAT GGAAACAGCT
    TTAAGTGCGC CTTCAAAAAT CCCCAAGCCA GCGAATGCAT GGGCATCTTC TCAACATTCA
    GTTTCATCCA AGTCATTCCG GGATTTCTTA GTAGAAGAAA AGAAATCTGT TACTGGCCAG
    AGATCAGGAG ATCATGTCAA AAAAGTTTGT TTTAAAGGGA TTGAAAATTC CCAGTCTCCA
    AAAGTTGTAA GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806685.1
    CDS354..4268
    Translation

    Target ORF information:

    RefSeq Version XM_010808383.2
    Organism Bos taurus(cattle)
    Definition Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808383.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCCAAAAT ACATTGTACA AATCTATATA GTTTTATACT GTTGCCAGGA GGATTTAAAT 
    TATAACCCTA AGCAGGCCGT TTATTCTCTA TCAGTCTTTC TGAATAGCAG CTTTGTGGCC
    TGGCTTTTTC ATTTTTTAAA ATTATCTGAC TATTCTGAAG AAGGAAGCTG CATAAAAACA
    AATAGGATGA ATTCCTCCAT CCCTGACTGC ACATCAAAGT GTCGCTCCCT GACGCATGCT
    TTGGATGTCC TTTCTGTGGT AACGAAGGGG AGTGAAAACC AGATTAAGGC CTTTCTATCC
    AGTCATTGTC ACAATGCTGC AACCATCAGG GATGCCTTTG GTCGGAATGC CCTCCACCTT
    GTTGCCTCCT GTGGGAAAAA AGGAGTGTTA GATTGGCTTA TTGAGAAAGG AGTGGATCCC
    TCGGTGAAAG ACAAGGAGTC AGGATGGACA GCTTTGCATA GAAGCATTTT TTATGGGCAT
    ATTGATTGTG TTTGGTCTCT ACTGAAGCAT GGTGTTAATC TGTATATTCA AGATAAAGAA
    GGCTTGTCAG CTTTGGATCT TGTAATGAAG GATAGACCAA CTCATGTAGT ATTCAAAAAT
    ACTGATCCTA CAGATGTCTA CACTTGGGGA GATAATACAA ATTTCACCCT GGGTCATGGA
    AGCCAGAATA GCAAACATCA TCCAGAGTTG GTGGATCTGT TCTCCAGGAG TGGGATTTAT
    ATCAAGCAGG TGGTGCTTTG TAAATTTCAC TCAGTGTTTC TGTCTCAGAA AGGGCAGGTT
    TACACCTGTG GTCATGGTCC TGGAGGACGA TTGGGCCATG GAGATGAGCA GACATGCTTG
    GTCCCTAGGC TTGTGGAAGG ACTGAGTGGT CATAATTGTT CTCAAGTAGC GGCTGCTAAG
    GATCATACTG TTGTATTAAC TGAAGATGGA TGTGTTTATA CATTTGGTCT AAATATTTTT
    CATCAATTAG GACTCATTCC TCCGCCTTCC AGTTGTAATG TACCCAGACA GATGCAGGCA
    AAGTACCTGA AAGGAAGGAC AATCATTGGA GTAGCAGCTG GCAGGTTTCA TACTGTGCTG
    TGGACTAGAG AGGCTGTTTA CACTATGGGA CTAAATGGTG GACAATTGGG TTATTTACTA
    GATCCCAATG GAGAAAAGTG TGTAACTGCT CCTCGTCAAG TCTCTGCCCT TCATCATAAG
    GACATCACTC TGTCTTTGGT TGCTGCTAGT GATGGGGCTA CTGTCTGTGT TACCACAAAG
    GGAGATATTT ACTTACTTGC AGACTATCAG TGCAAGAAGC TGGCTTCTAA ACAACTGAAC
    TTGAAAAAGG TTCTTGTATC TGGGGGGCAT ATGGAATACA AAGTTGATCC TGAACATTTG
    AAAGAAAATG GGGGTCAGAA AATTTGCATT CTTGCAATGG ATGGAGCTGG AAGGGTATTT
    TGCTGGAGAT CTGTCAACAG TTCTATGAAG CAGTGTCGAT GGGCTTATCC ACGCCAAGTC
    TTCATTTCTG ATATTGCTTT AAGTAGAAAT GAAATTCTTT TTGTCACACA AGATGGAGAG
    GGATTTAGAG GGAAATGGTT TGAAGAAAAA AGAAAGAGTT CTGAAAAGAA AGCAGAGATT
    TTATCAAACC TTCATAATTC CTCACCAGAT GTGTCTTGTG TCTCTGATAT AAATAGTGTA
    TATGAAAGAA TTCGACTTGA GAAACTTACT TTTGCCCATA GAGCTGTTAG TGTCAGCACA
    GATCCAAGTG GATGCAACTT TGCAGTCTTG CAGTCAGATC CTAAAACAAG CCTTTATGAA
    ATTCCAACTG TGTCCTCCTC ATCCTTTTTT GAGGAGTTTG GCAAGCTGTT GAGGGAAACG
    GATGAAATGG ACAACATCCA TGATGTGACA TTTCAAATTG GCAGTAGACT CTTCCCTGCA
    CATAAATATA TTTTGGCAGT GCGTTCTGAC TTTTTCCAGA AATTGTTTCT TTCGGATGGT
    ACCGCATTAG ACTTCACAGA TGTTTACCGG AAAGATGAAG ATTCTACAGG CTGCCATCTC
    TTTGTGGTAG AGAAGGTTCA TCCTGACCTG TTTGAATACC TTTTGCAGTT TATATACACA
    GATACTTGTG ACTTTTTAAC TCATGGCTTC AAACCAAGAC TAAATTTAAA CAGAAAACCA
    GAAGGATATC CAAGCACTCC AAACTGTCAT TCGAATAAAA TGAATTCCCA TGAAGATAAT
    CAGAAGTCAG CATTTGAAGC TTACAAAAGT AATCAAGCTC ATACAATTAG TGAAAAGCAG
    AAGAGTAAAT CCAAATCTTC CAAAAAAGTA AAAAGTGTTG GGGAAGATGA TCCTGTAAAA
    ATGTTGCAAA ATGTCGCAAA GAAATTTGGT TTCAGTAATT TAAGTAGTAG GTTAGATGGA
    GTCAGATATG AAAATGAAGA AATTAGTATC ATTGCCAAGA AAACTGGTAG TAAACCAAAG
    CTAAATCAGA AAAAATGTTC ATTTTTATGT GATGTGACCA TGAAATCAGT GGATGGAAAG
    GAATTTCCTT GTCATAAATG TGTTCTTTGT GCTAGACTTG AATATTTTCA TAGTATGCTG
    AGTAGCTCAT GGATTGAGGC TTCCAGTTGT GCAGCTCTGG AAATGCCAAT ACATTCTGAC
    ATACTGAAAG TTATTTTGGA CTACCTCTAT ACTGATGAAG CTGTTGTGAT AAAAGAATCT
    CAAAACGTGG ATTTTGTTTG TAGTGTTCTT GTGGTGGCTG ATCAGCTTCT CATATCTCGA
    TTGAAAGAAA TTTGTGAAGT AGCATTAACT GAAAAACTTA CCCTTAAGAA TGCTGCTATG
    CTACTGGAAT TTGCAGCAAT GTATAATGCT GAACAGTTGA AACTCTCTTG TTTACAGTTT
    ATTGGACTGA ATATGGCAGC TTTACTTGAA GCAAGATCTC TTGATGTTTT AAGTGATGGT
    GTTTTGAAGG ACCTTTCTGT GTTTTACCGG AAAATGATTC CAGCAATGGA GAGAAGAGTC
    ATTACACCAT ATCAAGATGG ACCAGATATT AGCTATTTGG ATGTAGAAGA TGGAGATATC
    TTTTTGAAAG AAGAAGTAAA TATGGAACAG AATTACTCGG AAAGTATGGT CAAGAAGACA
    AAAACAAAAG CAAAAAAGAA GCCACGTAAA CGATCAGATA GTTCTGGAGG TTATAATCTT
    TCAGATATTA TTCAGAGTCC ACCATCTACA GGATTGTTAA AGTCTGGTAA GACCAATTCT
    GTGGAGTCTC TTCCAGAACT GTTGACATCA GACTCTGAAG GAAGCTATGC AGGAGTGGGT
    AGTCCTAGAG ATTTACAGTC TCCTGACTTC ACAACAGGAT TTCATTCAGA TAAGTCTGAG
    GGGAAAATTA AATCGTATGT TAATGGTACA CCTCCTGCAT ATTTTAGGGA AGATTTAAAA
    CCATGGGAAA AATCACCAGT ACTTAAAATA ACTGCTCCAC AGCCCATTCC CAGTAACAGA
    ATTGATACTA CAAGCTCTTC CAATTGGGTG TCTGGCTCTT TCAGTCCTGT CAGCCCTCCT
    GTCATGGATC TCAGAACCAT CATGCAAATT GAAGAAAGCA GACAAAAATG TGGAACTACA
    CCAAAGACAA ATTTGGGAAA AATGATTTCT CATGGAGTTA AACTTTCTCA GAAGCAACGA
    AAAATGATTG CATTGACTAC CAAAGATAAT AATTCAGGAA TGAATAACAT GGAAACAGCT
    TTAAGTGCGC CTTCAAAAAT CCCCAAGCCA GCGAATGCAT GGGCATCTTC TCAACATTCA
    GTTTCATCCA AGTCATTCCG GGATTTCTTA GTAGAAGAAA AGAAATCTGT TACTGGCCAG
    AGATCAGGAG ATCATGTCAA AAAAGTTTGT TTTAAAGGGA TTGAAAATTC CCAGTCTCCA
    AAAGTTGTAA GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40388
    Clone ID Related Accession (Same CDS sequence) NM_001206040.1
    Accession Version NM_001206040.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product inhibitor of Bruton tyrosine kinase
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03053272.1, AAFC03125879.1, AAFC03024477.1 and AAFC03024479.1. On Apr 20, 2011 this sequence version replaced XM_613576.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCCT CCATCCCTGA CTGCACATCA AAGTGTCGCT CCCTGACGCA TGCTTTGGAT 
    GTCCTTTCTG TGGTAACGAA GGGGAGTGAA AACCAGATTA AGGCCTTTCT CTCCAGTCAT
    TGTCACAATG CTGCAACCAT CAGGGATGCC TTTGGTCGGA ATGCCCTCCA CCTTGTTGCC
    TCCTGTGGGA AAAAAGGAGT GTTAGATTGG CTTATTGAGA AAGGAGTGGA TCCCTCGGTG
    AAAGACAAGG AGTCAGGATG GACAGCTTTG CATAGAAGCA TTTTTTATGG GCATATTGAT
    TGTGTTTGGT CTCTACTGAA GCATGGTGTT AATCTGTATA TTCAAGATAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGATAGA CCAACTCATG TAGTATTCAA AAATACTGAT
    CCTACAGATG TCTACACTTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ATCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGAT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCACTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC
    TGTGGTCATG GTCCTGGAGG ACGATTGGGC CATGGAGATG AGCAGACATG CTTGGTCCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATAAT TGTTCTCAAG TAGCGGCTGC TAAGGATCAT
    ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACATTTG GTCTAAATAT TTTTCATCAA
    TTAGGACTCA TTCCTCCGCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC
    CTGAAAGGAA GGACAATCAT TGGAGTAGCA GCTGGCAGGT TTCATACTGT GCTGTGGACT
    AGAGAGGCTG TTTACACTAT GGGACTAAAT GGTGGACAAT TGGGTTATTT ACTAGATCCC
    AATGGAGAAA AGTGTGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGACATC
    ACTCTGTCTT TGGTTGCTGC TAGTGATGGG GCTACTGTCT GTGTTACCAC AAAGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGCTGGCTT CTAAACAACT GAACTTGAAA
    AAGGTTCTTG TATCTGGGGG GCATATGGAA TACAAAGTTG ATCCTGAACA TTTGAAAGAA
    AATGGGGGTC AGAAAATTTG CATTCTTGCA ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCTGTCA ACAGTTCTAT GAAGCAGTGT CGATGGGCTT ATCCACGCCA AGTCTTCATT
    TCTGATATTG CTTTAAGTAG AAATGAAATT CTTTTTGTCA CACAAGATGG AGAGGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAGAAAG AGTTCTGAAA AGAAAGAGAT TTTATCAAAC
    CTTCATAATT CCTCACCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATATGAAAGA
    ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT
    GGATGCAACT TTGCAGTCTT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAACT
    GTGTCCTCCT CATCCTTTTT TGAGGAGTTT GGCAAGCTGT TGAGGGAAAC GGATGAAATG
    GACAACATCC ATGATGTGAC ATTTCAAATT GGCAGTAGAC TCTTCCCTGC ACATAAATAT
    ATTTTGGCAG TGCGTTCTGA CTTTTTCCAG AAATTGTTTC TTTCGGATGG TACCGCATTA
    GACTTCACAG ATGTTTACCG GAAAGATGAA GATTCTACAG GCTGCCATCT CTTTGTGGTA
    GAGAAGGTTC ATCCTGACCT GTTTGAATAC CTTTTGCAGT TTATATACAC AGATACTTGT
    GACTTTTTAA CTCATGGCTT CAAACCAAGA CTAAATTTAA ACAGAAAACC AGAAGGATAT
    CCAAGCACTC CAAACTGTCA TTCGAATAAA ATGAATTCCC ATGAAGATAA TCAGAAGTCA
    GCATTTGAAG CTTACAAAAG TAATCAAGCT CATACAATTA GTGAAAAGCA GAAGAGTAAA
    TCCAAATCTT CCAAAAAAGT AAAAAGTGTT GGGGAAGATG ATCCTGTAAA AATGTTGCAA
    AATGTCGCAA AGAAATTTGG TTTCAGTAAT TTAAGTAGTA GGTTAGATGG AGTCAGATAT
    GAAAATGAAG AAATTAGTAT CATTGCCAAG AAAACTGGTA GTAAACCAAA GCTAAATCAG
    AAAAAATGTT CATTTTTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT
    TGTCATAAAT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA
    TGGATTGAGG CTTCCAGTTG TGCAGCTCTG GAAATGCCAA TACATTCTGA CATACTGAAA
    GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTTGTGA TAAAAGAATC TCAAAACGTG
    GATTTTGTTT GTAGTGTTCT TGTGGTGGCT GATCAGCTTC TCATATCTCG ATTGAAAGAA
    ATTTGTGAAG TAGCATTAAC TGAAAAACTT ACCCTTAAGA ATGCTGCTAT GCTACTGGAA
    TTTGCAGCAA TGTATAATGC TGAACAGTTG AAACTCTCTT GTTTACAGTT TATTGGACTG
    AATATGGCAG CTTTACTTGA AGCAAGATCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG
    GACCTTTCTG TGTTTTACCG GAAAATGATT CCAGCAATGG AGAGAAGAGT CATTACACCA
    TATCAAGATG GACCAGATAT TAGCTATTTG GATGTAGAAG ATGGAGATAT CTTTTTGAAA
    GAAGAAGTAA ATATGGAACA GAATTACTCG GAAAGTATGG TCAAGAAGAC AAAAACAAAA
    GCAAAAAAGA AGCCACGTAA ACGATCAGAT AGTTCTGGAG GTTATAATCT TTCAGATATT
    ATTCAGAGTC CACCATCTAC AGGATTGTTA AAGTCTGGTA AGACCAATTC TGTGGAGTCT
    CTTCCAGAAC TGTTGACATC AGACTCTGAA GGAAGCTATG CAGGAGTGGG TAGTCCTAGA
    GATTTACAGT CTCCTGACTT CACAACAGGA TTTCATTCAG ATAAGTCTGA GGGGAAAATT
    AAATCGTATG TTAATGGTAC ACCTCCTGCA TATTTTAGGG AAGATTTAAA ACCATGGGAA
    AAATCACCAG TACTTAAAAT AACTGCTCCA CAGCCCATTC CCAGTAACAG AATTGATACT
    ACAAGCTCTT CCAATTGGGT GTCTGGCTCT TTCAGTCCTG TCAGCCCTCC TGTCATGGAT
    CTCAGAACCA TCATGCAAAT TGAAGAAAGC AGACAAAAAT GTGGAACTAC ACCAAAGACA
    AATTTGGGAA AAATGATTTC TCATGGAGTT AAACTTTCTC AGAAGCAACG AAAAATGATT
    GCATTGACTA CCAAAGATAA TAATTCAGGA ATGAATAACA TGGAAACAGC TTTAAGTGCG
    CCTTCAAAAA TCCCCAAGCC AGCGAATGCA TGGGCATCTT CTCAACATTC AGTTTCATCC
    AAGTCATTCC GGGATTTCTT AGTAGAAGAA AAGAAATCTG TTACTGGCCA GAGATCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGG ATTGAAAATT CCCAGTCTCC AAAAGTTGTA
    AGATGTTCTG CTCATGGTTC CCCAGGACGA GAAGGCAACC ACATTTCAGA TGTACCACTT
    CCGGACAGTC CCAATCCCTG GCTGTCTTCT TCACTGACCG CTCCTTCCGT GGTAGCACCA
    GTCACTTTTG CGTCTATCGT AGAAGAAGAG CTACAGCAGG AAGCAGCTCT TATCAGAAGC
    CGAGAGAAAC CCTTGGCTTT GATTCAGATT GAGGAGCGTG CAATACAGGA TTTGTTGGTT
    TTCTATGAAG CATTTGGAAA CCCTGATGAG TTTGTCATTG TTGAAAGGAC ACCACAGGGA
    CCACTGGCAG TACCTATGTG GAATAAGCAT GGCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192969.1
    CDS1..4059
    Translation

    Target ORF information:

    RefSeq Version NM_001206040.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206040.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTCCT CCATCCCTGA CTGCACATCA AAGTGTCGCT CCCTGACGCA TGCTTTGGAT 
    GTCCTTTCTG TGGTAACGAA GGGGAGTGAA AACCAGATTA AGGCCTTTCT CTCCAGTCAT
    TGTCACAATG CTGCAACCAT CAGGGATGCC TTTGGTCGGA ATGCCCTCCA CCTTGTTGCC
    TCCTGTGGGA AAAAAGGAGT GTTAGATTGG CTTATTGAGA AAGGAGTGGA TCCCTCGGTG
    AAAGACAAGG AGTCAGGATG GACAGCTTTG CATAGAAGCA TTTTTTATGG GCATATTGAT
    TGTGTTTGGT CTCTACTGAA GCATGGTGTT AATCTGTATA TTCAAGATAA AGAAGGCTTG
    TCAGCTTTGG ATCTTGTAAT GAAGGATAGA CCAACTCATG TAGTATTCAA AAATACTGAT
    CCTACAGATG TCTACACTTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG
    AATAGCAAAC ATCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGAT TTATATCAAG
    CAGGTGGTGC TTTGTAAATT TCACTCAGTG TTTCTGTCTC AGAAAGGGCA GGTTTACACC
    TGTGGTCATG GTCCTGGAGG ACGATTGGGC CATGGAGATG AGCAGACATG CTTGGTCCCT
    AGGCTTGTGG AAGGACTGAG TGGTCATAAT TGTTCTCAAG TAGCGGCTGC TAAGGATCAT
    ACTGTTGTAT TAACTGAAGA TGGATGTGTT TATACATTTG GTCTAAATAT TTTTCATCAA
    TTAGGACTCA TTCCTCCGCC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAGTAC
    CTGAAAGGAA GGACAATCAT TGGAGTAGCA GCTGGCAGGT TTCATACTGT GCTGTGGACT
    AGAGAGGCTG TTTACACTAT GGGACTAAAT GGTGGACAAT TGGGTTATTT ACTAGATCCC
    AATGGAGAAA AGTGTGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGACATC
    ACTCTGTCTT TGGTTGCTGC TAGTGATGGG GCTACTGTCT GTGTTACCAC AAAGGGAGAT
    ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGCTGGCTT CTAAACAACT GAACTTGAAA
    AAGGTTCTTG TATCTGGGGG GCATATGGAA TACAAAGTTG ATCCTGAACA TTTGAAAGAA
    AATGGGGGTC AGAAAATTTG CATTCTTGCA ATGGATGGAG CTGGAAGGGT ATTTTGCTGG
    AGATCTGTCA ACAGTTCTAT GAAGCAGTGT CGATGGGCTT ATCCACGCCA AGTCTTCATT
    TCTGATATTG CTTTAAGTAG AAATGAAATT CTTTTTGTCA CACAAGATGG AGAGGGATTT
    AGAGGGAAAT GGTTTGAAGA AAAAAGAAAG AGTTCTGAAA AGAAAGAGAT TTTATCAAAC
    CTTCATAATT CCTCACCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATATGAAAGA
    ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT
    GGATGCAACT TTGCAGTCTT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAACT
    GTGTCCTCCT CATCCTTTTT TGAGGAGTTT GGCAAGCTGT TGAGGGAAAC GGATGAAATG
    GACAACATCC ATGATGTGAC ATTTCAAATT GGCAGTAGAC TCTTCCCTGC ACATAAATAT
    ATTTTGGCAG TGCGTTCTGA CTTTTTCCAG AAATTGTTTC TTTCGGATGG TACCGCATTA
    GACTTCACAG ATGTTTACCG GAAAGATGAA GATTCTACAG GCTGCCATCT CTTTGTGGTA
    GAGAAGGTTC ATCCTGACCT GTTTGAATAC CTTTTGCAGT TTATATACAC AGATACTTGT
    GACTTTTTAA CTCATGGCTT CAAACCAAGA CTAAATTTAA ACAGAAAACC AGAAGGATAT
    CCAAGCACTC CAAACTGTCA TTCGAATAAA ATGAATTCCC ATGAAGATAA TCAGAAGTCA
    GCATTTGAAG CTTACAAAAG TAATCAAGCT CATACAATTA GTGAAAAGCA GAAGAGTAAA
    TCCAAATCTT CCAAAAAAGT AAAAAGTGTT GGGGAAGATG ATCCTGTAAA AATGTTGCAA
    AATGTCGCAA AGAAATTTGG TTTCAGTAAT TTAAGTAGTA GGTTAGATGG AGTCAGATAT
    GAAAATGAAG AAATTAGTAT CATTGCCAAG AAAACTGGTA GTAAACCAAA GCTAAATCAG
    AAAAAATGTT CATTTTTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT
    TGTCATAAAT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA
    TGGATTGAGG CTTCCAGTTG TGCAGCTCTG GAAATGCCAA TACATTCTGA CATACTGAAA
    GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTTGTGA TAAAAGAATC TCAAAACGTG
    GATTTTGTTT GTAGTGTTCT TGTGGTGGCT GATCAGCTTC TCATATCTCG ATTGAAAGAA
    ATTTGTGAAG TAGCATTAAC TGAAAAACTT ACCCTTAAGA ATGCTGCTAT GCTACTGGAA
    TTTGCAGCAA TGTATAATGC TGAACAGTTG AAACTCTCTT GTTTACAGTT TATTGGACTG
    AATATGGCAG CTTTACTTGA AGCAAGATCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG
    GACCTTTCTG TGTTTTACCG GAAAATGATT CCAGCAATGG AGAGAAGAGT CATTACACCA
    TATCAAGATG GACCAGATAT TAGCTATTTG GATGTAGAAG ATGGAGATAT CTTTTTGAAA
    GAAGAAGTAA ATATGGAACA GAATTACTCG GAAAGTATGG TCAAGAAGAC AAAAACAAAA
    GCAAAAAAGA AGCCACGTAA ACGATCAGAT AGTTCTGGAG GTTATAATCT TTCAGATATT
    ATTCAGAGTC CACCATCTAC AGGATTGTTA AAGTCTGGTA AGACCAATTC TGTGGAGTCT
    CTTCCAGAAC TGTTGACATC AGACTCTGAA GGAAGCTATG CAGGAGTGGG TAGTCCTAGA
    GATTTACAGT CTCCTGACTT CACAACAGGA TTTCATTCAG ATAAGTCTGA GGGGAAAATT
    AAATCGTATG TTAATGGTAC ACCTCCTGCA TATTTTAGGG AAGATTTAAA ACCATGGGAA
    AAATCACCAG TACTTAAAAT AACTGCTCCA CAGCCCATTC CCAGTAACAG AATTGATACT
    ACAAGCTCTT CCAATTGGGT GTCTGGCTCT TTCAGTCCTG TCAGCCCTCC TGTCATGGAT
    CTCAGAACCA TCATGCAAAT TGAAGAAAGC AGACAAAAAT GTGGAACTAC ACCAAAGACA
    AATTTGGGAA AAATGATTTC TCATGGAGTT AAACTTTCTC AGAAGCAACG AAAAATGATT
    GCATTGACTA CCAAAGATAA TAATTCAGGA ATGAATAACA TGGAAACAGC TTTAAGTGCG
    CCTTCAAAAA TCCCCAAGCC AGCGAATGCA TGGGCATCTT CTCAACATTC AGTTTCATCC
    AAGTCATTCC GGGATTTCTT AGTAGAAGAA AAGAAATCTG TTACTGGCCA GAGATCAGGA
    GATCATGTCA AAAAAGTTTG TTTTAAAGGG ATTGAAAATT CCCAGTCTCC AAAAGTTGTA
    AGATGTTCTG CTCATGGTTC CCCAGGACGA GAAGGCAACC ACATTTCAGA TGTACCACTT
    CCGGACAGTC CCAATCCCTG GCTGTCTTCT TCACTGACCG CTCCTTCCGT GGTAGCACCA
    GTCACTTTTG CGTCTATCGT AGAAGAAGAG CTACAGCAGG AAGCAGCTCT TATCAGAAGC
    CGAGAGAAAC CCTTGGCTTT GATTCAGATT GAGGAGCGTG CAATACAGGA TTTGTTGGTT
    TTCTATGAAG CATTTGGAAA CCCTGATGAG TTTGTCATTG TTGAAAGGAC ACCACAGGGA
    CCACTGGCAG TACCTATGTG GAATAAGCAT GGCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22493
    Clone ID Related Accession (Same CDS sequence) XM_005210695.3
    Accession Version XM_005210695.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4248bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inhibitor of Bruton tyrosine kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104175.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210695.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCAAAAT ACATTGTACA AATCTATATA GTTTTATACT GTTGCCAGGA GGATTTAAAT 
    TATAACCCTA AGCAGGCCGT TTATTCTCTA TCAGTCTTTC TGAATAGCAG CTTTGTGGCC
    TGGCTTTTTC ATTTTTTAAA ATTATCTGAC TATTCTGAAG AAGGAAGCTG CATAAAAACA
    AATAGGATGA ATTCCTCCAT CCCTGACTGC ACATCAAAGT GTCGCTCCCT GACGCATGCT
    TTGGATGTCC TTTCTGTGGT AACGAAGGGG AGTGAAAACC AGATTAAGGC CTTTCTATCC
    AGTCATTGTC ACAATGCTGC AACCATCAGG GATGCCTTTG GTCGGAATGC CCTCCACCTT
    GTTGCCTCCT GTGGGAAAAA AGGAGTGTTA GATTGGCTTA TTGAGAAAGG AGTGGATCCC
    TCGGTGAAAG ACAAGGAGTC AGGATGGACA GCTTTGCATA GAAGCATTTT TTATGGGCAT
    ATTGATTGTG TTTGGTCTCT ACTGAAGCAT GGTGTTAATC TGTATATTCA AGATAAAGAA
    GGCTTGTCAG CTTTGGATCT TGTAATGAAG GATAGACCAA CTCATGTAGT ATTCAAAAAT
    ACTGATCCTA CAGATGTCTA CACTTGGGGA GATAATACAA ATTTCACCCT GGGTCATGGA
    AGCCAGAATA GCAAACATCA TCCAGAGTTG GTGGATCTGT TCTCCAGGAG TGGGATTTAT
    ATCAAGCAGG TGGTGCTTTG TAAATTTCAC TCAGTGTTTC TGTCTCAGAA AGGGCAGGTT
    TACACCTGTG GTCATGGTCC TGGAGGACGA TTGGGCCATG GAGATGAGCA GACATGCTTG
    GTCCCTAGGC TTGTGGAAGG ACTGAGTGGT CATAATTGTT CTCAAGTAGC GGCTGCTAAG
    GATCATACTG TTGTATTAAC TGAAGATGGA TGTGTTTATA CATTTGGTCT AAATATTTTT
    CATCAATTAG GACTCATTCC TCCGCCTTCC AGTTGTAATG TACCCAGACA GATGCAGGCA
    AAGTACCTGA AAGGAAGGAC AATCATTGGA GTAGCAGCTG GCAGGTTTCA TACTGTGCTG
    TGGACTAGAG AGGCTGTTTA CACTATGGGA CTAAATGGTG GACAATTGGG TTATTTACTA
    GATCCCAATG GAGAAAAGTG TGTAACTGCT CCTCGTCAAG TCTCTGCCCT TCATCATAAG
    GACATCACTC TGTCTTTGGT TGCTGCTAGT GATGGGGCTA CTGTCTGTGT TACCACAAAG
    GGAGATATTT ACTTACTTGC AGACTATCAG TGCAAGAAGC TGGCTTCTAA ACAACTGAAC
    TTGAAAAAGG TTCTTGTATC TGGGGGGCAT ATGGAATACA AAGTTGATCC TGAACATTTG
    AAAGAAAATG GGGGTCAGAA AATTTGCATT CTTGCAATGG ATGGAGCTGG AAGGGTATTT
    TGCTGGAGAT CTGTCAACAG TTCTATGAAG CAGTGTCGAT GGGCTTATCC ACGCCAAGTC
    TTCATTTCTG ATATTGCTTT AAGTAGAAAT GAAATTCTTT TTGTCACACA AGATGGAGAG
    GGATTTAGAG GGAAATGGTT TGAAGAAAAA AGAAAGAGTT CTGAAAAGAA AGCAGAGATT
    TTATCAAACC TTCATAATTC CTCACCAGAT GTGTCTTGTG TCTCTGATAT AAATAGTGTA
    TATGAAAGAA TTCGACTTGA GAAACTTACT TTTGCCCATA GAGCTGTTAG TGTCAGCACA
    GATCCAAGTG GATGCAACTT TGCAGTCTTG CAGTCAGATC CTAAAACAAG CCTTTATGAA
    ATTCCAACTG TGTCCTCCTC ATCCTTTTTT GAGGAGTTTG GCAAGCTGTT GAGGGAAACG
    GATGAAATGG ACAACATCCA TGATGTGACA TTTCAAATTG GCAGTAGACT CTTCCCTGCA
    CATAAATATA TTTTGGCAGT GCGTTCTGAC TTTTTCCAGA AATTGTTTCT TTCGGATGGT
    ACCGCATTAG ACTTCACAGA TGTTTACCGG AAAGATGAAG ATTCTACAGG CTGCCATCTC
    TTTGTGGTAG AGAAGGTTCA TCCTGACCTG TTTGAATACC TTTTGCAGTT TATATACACA
    GATACTTGTG ACTTTTTAAC TCATGGCTTC AAACCAAGAC TAAATTTAAA CAGAAAACCA
    GAAGGATATC CAAGCACTCC AAACTGTCAT TCGAATAAAA TGAATTCCCA TGAAGATAAT
    CAGAAGTCAG CATTTGAAGC TTACAAAAGT AATCAAGCTC ATACAATTAG TGAAAAGCAG
    AAGAGTAAAT CCAAATCTTC CAAAAAAGTA AAAAGTGTTG GGGAAGATGA TCCTGTAAAA
    ATGTTGCAAA ATGTCGCAAA GAAATTTGGT TTCAGTAATT TAAGTAGTAG GTTAGATGGA
    GTCAGATATG AAAATGAAGA AATTAGTATC ATTGCCAAGA AAACTGGTAG TAAACCAAAG
    CTAAATCAGA AAAAATGTTC ATTTTTATGT GATGTGACCA TGAAATCAGT GGATGGAAAG
    GAATTTCCTT GTCATAAATG TGTTCTTTGT GCTAGACTTG AATATTTTCA TAGTATGCTG
    AGTAGCTCAT GGATTGAGGC TTCCAGTTGT GCAGCTCTGG AAATGCCAAT ACATTCTGAC
    ATACTGAAAG TTATTTTGGA CTACCTCTAT ACTGATGAAG CTGTTGTGAT AAAAGAATCT
    CAAAACGTGG ATTTTGTTTG TAGTGTTCTT GTGGTGGCTG ATCAGCTTCT CATATCTCGA
    TTGAAAGAAA TTTGTGAAGT AGCATTAACT GAAAAACTTA CCCTTAAGAA TGCTGCTATG
    CTACTGGAAT TTGCAGCAAT GTATAATGCT GAACAGTTGA AACTCTCTTG TTTACAGTTT
    ATTGGACTGA ATATGGCAGC TTTACTTGAA GCAAGATCTC TTGATGTTTT AAGTGATGGT
    GTTTTGAAGG ACCTTTCTGT GTTTTACCGG AAAATGATTC CAGCAATGGA GAGAAGAGTC
    ATTACACCAT ATCAAGATGG ACCAGATATT AGCTATTTGG ATGTAGAAGA TGGAGATATC
    TTTTTGAAAG AAGAAGTAAA TATGGAACAG AATTACTCGG AAAGTATGGT CAAGAAGACA
    AAAACAAAAG CAAAAAAGAA GCCACGTAAA CGATCAGATA GTTCTGGAGG TTATAATCTT
    TCAGATATTA TTCAGAGTCC ACCATCTACA GGATTGTTAA AGTCTGGTAA GACCAATTCT
    GTGGAGTCTC TTCCAGAACT GTTGACATCA GACTCTGAAG GAAGCTATGC AGGAGTGGGT
    AGTCCTAGAG ATTTACAGTC TCCTGACTTC ACAACAGGAT TTCATTCAGA TAAGTCTGAG
    GGGAAAATTA AATCGTATGT TAATGGTACA CCTCCTGCAT ATTTTAGGGA AGATTTAAAA
    CCATGGGAAA AATCACCAGT ACTTAAAATA ACTGCTCCAC AGCCCATTCC CAGTAACAGA
    ATTGATACTA CAAGCTCTTC CAATTGGGTG TCTGGCTCTT TCAGTCCTGT CAGCCCTCCT
    GTCATGGATC TCAGAACCAT CATGCAAATT GAAGAAAGCA GACAAAAATG TGGAACTACA
    CCAAAGACAA ATTTGGGAAA AATGATTTCT CATGGAGTTA AACTTTCTCA GAAGCAACGA
    AAAATGATTG CATTGACTAC CAAAGATAAT AATTCAGGAA TGAATAACAT GGAAACAGCT
    TTAAGTGCGC CTTCAAAAAT CCCCAAGCCA GCGAATGCAT GGGCATCTTC TCAACATTCA
    GTTTCATCCA AGTCATTCCG GGATTTCTTA GTAGAAGAAA AGAAATCTGT TACTGGCCAG
    AGATCAGGAG ATCATGTCAA AAAAGTTTGT TTTAAAGGGA TTGAAAATTC CCAGTCTCCA
    AAAGTTGTAA GATGTTCTGC TCATGGTTCC CCAGGACGAG AAGGCAACCA CATTTCAGAT
    GTACCACTTC CGGACAGTCC CAATCCCTGG CTGTCTTCTT CACTGACCGC TCCTTCCGTG
    GTAGCACCAG TCACTTTTGC GTCTATCGTA GAAGAAGAGC TACAGCAGGA AGCAGCTCTT
    ATCAGAAGCC GAGAGAAACC CTTGGCTTTG ATTCAGATTG AGGAGCGTGC AATACAGGAT
    TTGTTGGTTT TCTATGAAGC ATTTGGAAAC CCTGATGAGT TTGTCATTGT TGAAAGGACA
    CCACAGGGAC CACTGGCAGT ACCTATGTGG AATAAGCATG GCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210752.1
    CDS352..4599
    Translation

    Target ORF information:

    RefSeq Version XM_005210695.3
    Organism Bos taurus(cattle)
    Definition Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210695.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCAAAAT ACATTGTACA AATCTATATA GTTTTATACT GTTGCCAGGA GGATTTAAAT 
    TATAACCCTA AGCAGGCCGT TTATTCTCTA TCAGTCTTTC TGAATAGCAG CTTTGTGGCC
    TGGCTTTTTC ATTTTTTAAA ATTATCTGAC TATTCTGAAG AAGGAAGCTG CATAAAAACA
    AATAGGATGA ATTCCTCCAT CCCTGACTGC ACATCAAAGT GTCGCTCCCT GACGCATGCT
    TTGGATGTCC TTTCTGTGGT AACGAAGGGG AGTGAAAACC AGATTAAGGC CTTTCTATCC
    AGTCATTGTC ACAATGCTGC AACCATCAGG GATGCCTTTG GTCGGAATGC CCTCCACCTT
    GTTGCCTCCT GTGGGAAAAA AGGAGTGTTA GATTGGCTTA TTGAGAAAGG AGTGGATCCC
    TCGGTGAAAG ACAAGGAGTC AGGATGGACA GCTTTGCATA GAAGCATTTT TTATGGGCAT
    ATTGATTGTG TTTGGTCTCT ACTGAAGCAT GGTGTTAATC TGTATATTCA AGATAAAGAA
    GGCTTGTCAG CTTTGGATCT TGTAATGAAG GATAGACCAA CTCATGTAGT ATTCAAAAAT
    ACTGATCCTA CAGATGTCTA CACTTGGGGA GATAATACAA ATTTCACCCT GGGTCATGGA
    AGCCAGAATA GCAAACATCA TCCAGAGTTG GTGGATCTGT TCTCCAGGAG TGGGATTTAT
    ATCAAGCAGG TGGTGCTTTG TAAATTTCAC TCAGTGTTTC TGTCTCAGAA AGGGCAGGTT
    TACACCTGTG GTCATGGTCC TGGAGGACGA TTGGGCCATG GAGATGAGCA GACATGCTTG
    GTCCCTAGGC TTGTGGAAGG ACTGAGTGGT CATAATTGTT CTCAAGTAGC GGCTGCTAAG
    GATCATACTG TTGTATTAAC TGAAGATGGA TGTGTTTATA CATTTGGTCT AAATATTTTT
    CATCAATTAG GACTCATTCC TCCGCCTTCC AGTTGTAATG TACCCAGACA GATGCAGGCA
    AAGTACCTGA AAGGAAGGAC AATCATTGGA GTAGCAGCTG GCAGGTTTCA TACTGTGCTG
    TGGACTAGAG AGGCTGTTTA CACTATGGGA CTAAATGGTG GACAATTGGG TTATTTACTA
    GATCCCAATG GAGAAAAGTG TGTAACTGCT CCTCGTCAAG TCTCTGCCCT TCATCATAAG
    GACATCACTC TGTCTTTGGT TGCTGCTAGT GATGGGGCTA CTGTCTGTGT TACCACAAAG
    GGAGATATTT ACTTACTTGC AGACTATCAG TGCAAGAAGC TGGCTTCTAA ACAACTGAAC
    TTGAAAAAGG TTCTTGTATC TGGGGGGCAT ATGGAATACA AAGTTGATCC TGAACATTTG
    AAAGAAAATG GGGGTCAGAA AATTTGCATT CTTGCAATGG ATGGAGCTGG AAGGGTATTT
    TGCTGGAGAT CTGTCAACAG TTCTATGAAG CAGTGTCGAT GGGCTTATCC ACGCCAAGTC
    TTCATTTCTG ATATTGCTTT AAGTAGAAAT GAAATTCTTT TTGTCACACA AGATGGAGAG
    GGATTTAGAG GGAAATGGTT TGAAGAAAAA AGAAAGAGTT CTGAAAAGAA AGCAGAGATT
    TTATCAAACC TTCATAATTC CTCACCAGAT GTGTCTTGTG TCTCTGATAT AAATAGTGTA
    TATGAAAGAA TTCGACTTGA GAAACTTACT TTTGCCCATA GAGCTGTTAG TGTCAGCACA
    GATCCAAGTG GATGCAACTT TGCAGTCTTG CAGTCAGATC CTAAAACAAG CCTTTATGAA
    ATTCCAACTG TGTCCTCCTC ATCCTTTTTT GAGGAGTTTG GCAAGCTGTT GAGGGAAACG
    GATGAAATGG ACAACATCCA TGATGTGACA TTTCAAATTG GCAGTAGACT CTTCCCTGCA
    CATAAATATA TTTTGGCAGT GCGTTCTGAC TTTTTCCAGA AATTGTTTCT TTCGGATGGT
    ACCGCATTAG ACTTCACAGA TGTTTACCGG AAAGATGAAG ATTCTACAGG CTGCCATCTC
    TTTGTGGTAG AGAAGGTTCA TCCTGACCTG TTTGAATACC TTTTGCAGTT TATATACACA
    GATACTTGTG ACTTTTTAAC TCATGGCTTC AAACCAAGAC TAAATTTAAA CAGAAAACCA
    GAAGGATATC CAAGCACTCC AAACTGTCAT TCGAATAAAA TGAATTCCCA TGAAGATAAT
    CAGAAGTCAG CATTTGAAGC TTACAAAAGT AATCAAGCTC ATACAATTAG TGAAAAGCAG
    AAGAGTAAAT CCAAATCTTC CAAAAAAGTA AAAAGTGTTG GGGAAGATGA TCCTGTAAAA
    ATGTTGCAAA ATGTCGCAAA GAAATTTGGT TTCAGTAATT TAAGTAGTAG GTTAGATGGA
    GTCAGATATG AAAATGAAGA AATTAGTATC ATTGCCAAGA AAACTGGTAG TAAACCAAAG
    CTAAATCAGA AAAAATGTTC ATTTTTATGT GATGTGACCA TGAAATCAGT GGATGGAAAG
    GAATTTCCTT GTCATAAATG TGTTCTTTGT GCTAGACTTG AATATTTTCA TAGTATGCTG
    AGTAGCTCAT GGATTGAGGC TTCCAGTTGT GCAGCTCTGG AAATGCCAAT ACATTCTGAC
    ATACTGAAAG TTATTTTGGA CTACCTCTAT ACTGATGAAG CTGTTGTGAT AAAAGAATCT
    CAAAACGTGG ATTTTGTTTG TAGTGTTCTT GTGGTGGCTG ATCAGCTTCT CATATCTCGA
    TTGAAAGAAA TTTGTGAAGT AGCATTAACT GAAAAACTTA CCCTTAAGAA TGCTGCTATG
    CTACTGGAAT TTGCAGCAAT GTATAATGCT GAACAGTTGA AACTCTCTTG TTTACAGTTT
    ATTGGACTGA ATATGGCAGC TTTACTTGAA GCAAGATCTC TTGATGTTTT AAGTGATGGT
    GTTTTGAAGG ACCTTTCTGT GTTTTACCGG AAAATGATTC CAGCAATGGA GAGAAGAGTC
    ATTACACCAT ATCAAGATGG ACCAGATATT AGCTATTTGG ATGTAGAAGA TGGAGATATC
    TTTTTGAAAG AAGAAGTAAA TATGGAACAG AATTACTCGG AAAGTATGGT CAAGAAGACA
    AAAACAAAAG CAAAAAAGAA GCCACGTAAA CGATCAGATA GTTCTGGAGG TTATAATCTT
    TCAGATATTA TTCAGAGTCC ACCATCTACA GGATTGTTAA AGTCTGGTAA GACCAATTCT
    GTGGAGTCTC TTCCAGAACT GTTGACATCA GACTCTGAAG GAAGCTATGC AGGAGTGGGT
    AGTCCTAGAG ATTTACAGTC TCCTGACTTC ACAACAGGAT TTCATTCAGA TAAGTCTGAG
    GGGAAAATTA AATCGTATGT TAATGGTACA CCTCCTGCAT ATTTTAGGGA AGATTTAAAA
    CCATGGGAAA AATCACCAGT ACTTAAAATA ACTGCTCCAC AGCCCATTCC CAGTAACAGA
    ATTGATACTA CAAGCTCTTC CAATTGGGTG TCTGGCTCTT TCAGTCCTGT CAGCCCTCCT
    GTCATGGATC TCAGAACCAT CATGCAAATT GAAGAAAGCA GACAAAAATG TGGAACTACA
    CCAAAGACAA ATTTGGGAAA AATGATTTCT CATGGAGTTA AACTTTCTCA GAAGCAACGA
    AAAATGATTG CATTGACTAC CAAAGATAAT AATTCAGGAA TGAATAACAT GGAAACAGCT
    TTAAGTGCGC CTTCAAAAAT CCCCAAGCCA GCGAATGCAT GGGCATCTTC TCAACATTCA
    GTTTCATCCA AGTCATTCCG GGATTTCTTA GTAGAAGAAA AGAAATCTGT TACTGGCCAG
    AGATCAGGAG ATCATGTCAA AAAAGTTTGT TTTAAAGGGA TTGAAAATTC CCAGTCTCCA
    AAAGTTGTAA GATGTTCTGC TCATGGTTCC CCAGGACGAG AAGGCAACCA CATTTCAGAT
    GTACCACTTC CGGACAGTCC CAATCCCTGG CTGTCTTCTT CACTGACCGC TCCTTCCGTG
    GTAGCACCAG TCACTTTTGC GTCTATCGTA GAAGAAGAGC TACAGCAGGA AGCAGCTCTT
    ATCAGAAGCC GAGAGAAACC CTTGGCTTTG ATTCAGATTG AGGAGCGTGC AATACAGGAT
    TTGTTGGTTT TCTATGAAGC ATTTGGAAAC CCTGATGAGT TTGTCATTGT TGAAAGGACA
    CCACAGGGAC CACTGGCAGT ACCTATGTGG AATAAGCATG GCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22494
    Clone ID Related Accession (Same CDS sequence) XM_005210696.3
    Accession Version XM_005210696.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4245bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product inhibitor of Bruton tyrosine kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104175.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210696.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCAAAAT ACATTGTACA AATCTATATA GTTTTATACT GTTGCCAGGA GGATTTAAAT 
    TATAACCCTA AGCAGGCCGT TTATTCTCTA TCAGTCTTTC TGAATAGCAG CTTTGTGGCC
    TGGCTTTTTC ATTTTTTAAA ATTATCTGAC TATTCTGAAG AAGGAAGCTG CATAAAAACA
    AATAGGATGA ATTCCTCCAT CCCTGACTGC ACATCAAAGT GTCGCTCCCT GACGCATGCT
    TTGGATGTCC TTTCTGTGGT AACGAAGGGG AGTGAAAACC AGATTAAGGC CTTTCTATCC
    AGTCATTGTC ACAATGCTGC AACCATCAGG GATGCCTTTG GTCGGAATGC CCTCCACCTT
    GTTGCCTCCT GTGGGAAAAA AGGAGTGTTA GATTGGCTTA TTGAGAAAGG AGTGGATCCC
    TCGGTGAAAG ACAAGGAGTC AGGATGGACA GCTTTGCATA GAAGCATTTT TTATGGGCAT
    ATTGATTGTG TTTGGTCTCT ACTGAAGCAT GGTGTTAATC TGTATATTCA AGATAAAGAA
    GGCTTGTCAG CTTTGGATCT TGTAATGAAG GATAGACCAA CTCATGTAGT ATTCAAAAAT
    ACTGATCCTA CAGATGTCTA CACTTGGGGA GATAATACAA ATTTCACCCT GGGTCATGGA
    AGCCAGAATA GCAAACATCA TCCAGAGTTG GTGGATCTGT TCTCCAGGAG TGGGATTTAT
    ATCAAGCAGG TGGTGCTTTG TAAATTTCAC TCAGTGTTTC TGTCTCAGAA AGGGCAGGTT
    TACACCTGTG GTCATGGTCC TGGAGGACGA TTGGGCCATG GAGATGAGCA GACATGCTTG
    GTCCCTAGGC TTGTGGAAGG ACTGAGTGGT CATAATTGTT CTCAAGTAGC GGCTGCTAAG
    GATCATACTG TTGTATTAAC TGAAGATGGA TGTGTTTATA CATTTGGTCT AAATATTTTT
    CATCAATTAG GACTCATTCC TCCGCCTTCC AGTTGTAATG TACCCAGACA GATGCAGGCA
    AAGTACCTGA AAGGAAGGAC AATCATTGGA GTAGCAGCTG GCAGGTTTCA TACTGTGCTG
    TGGACTAGAG AGGCTGTTTA CACTATGGGA CTAAATGGTG GACAATTGGG TTATTTACTA
    GATCCCAATG GAGAAAAGTG TGTAACTGCT CCTCGTCAAG TCTCTGCCCT TCATCATAAG
    GACATCACTC TGTCTTTGGT TGCTGCTAGT GATGGGGCTA CTGTCTGTGT TACCACAAAG
    GGAGATATTT ACTTACTTGC AGACTATCAG TGCAAGAAGC TGGCTTCTAA ACAACTGAAC
    TTGAAAAAGG TTCTTGTATC TGGGGGGCAT ATGGAATACA AAGTTGATCC TGAACATTTG
    AAAGAAAATG GGGGTCAGAA AATTTGCATT CTTGCAATGG ATGGAGCTGG AAGGGTATTT
    TGCTGGAGAT CTGTCAACAG TTCTATGAAG CAGTGTCGAT GGGCTTATCC ACGCCAAGTC
    TTCATTTCTG ATATTGCTTT AAGTAGAAAT GAAATTCTTT TTGTCACACA AGATGGAGAG
    GGATTTAGAG GGAAATGGTT TGAAGAAAAA AGAAAGAGTT CTGAAAAGAA AGAGATTTTA
    TCAAACCTTC ATAATTCCTC ACCAGATGTG TCTTGTGTCT CTGATATAAA TAGTGTATAT
    GAAAGAATTC GACTTGAGAA ACTTACTTTT GCCCATAGAG CTGTTAGTGT CAGCACAGAT
    CCAAGTGGAT GCAACTTTGC AGTCTTGCAG TCAGATCCTA AAACAAGCCT TTATGAAATT
    CCAACTGTGT CCTCCTCATC CTTTTTTGAG GAGTTTGGCA AGCTGTTGAG GGAAACGGAT
    GAAATGGACA ACATCCATGA TGTGACATTT CAAATTGGCA GTAGACTCTT CCCTGCACAT
    AAATATATTT TGGCAGTGCG TTCTGACTTT TTCCAGAAAT TGTTTCTTTC GGATGGTACC
    GCATTAGACT TCACAGATGT TTACCGGAAA GATGAAGATT CTACAGGCTG CCATCTCTTT
    GTGGTAGAGA AGGTTCATCC TGACCTGTTT GAATACCTTT TGCAGTTTAT ATACACAGAT
    ACTTGTGACT TTTTAACTCA TGGCTTCAAA CCAAGACTAA ATTTAAACAG AAAACCAGAA
    GGATATCCAA GCACTCCAAA CTGTCATTCG AATAAAATGA ATTCCCATGA AGATAATCAG
    AAGTCAGCAT TTGAAGCTTA CAAAAGTAAT CAAGCTCATA CAATTAGTGA AAAGCAGAAG
    AGTAAATCCA AATCTTCCAA AAAAGTAAAA AGTGTTGGGG AAGATGATCC TGTAAAAATG
    TTGCAAAATG TCGCAAAGAA ATTTGGTTTC AGTAATTTAA GTAGTAGGTT AGATGGAGTC
    AGATATGAAA ATGAAGAAAT TAGTATCATT GCCAAGAAAA CTGGTAGTAA ACCAAAGCTA
    AATCAGAAAA AATGTTCATT TTTATGTGAT GTGACCATGA AATCAGTGGA TGGAAAGGAA
    TTTCCTTGTC ATAAATGTGT TCTTTGTGCT AGACTTGAAT ATTTTCATAG TATGCTGAGT
    AGCTCATGGA TTGAGGCTTC CAGTTGTGCA GCTCTGGAAA TGCCAATACA TTCTGACATA
    CTGAAAGTTA TTTTGGACTA CCTCTATACT GATGAAGCTG TTGTGATAAA AGAATCTCAA
    AACGTGGATT TTGTTTGTAG TGTTCTTGTG GTGGCTGATC AGCTTCTCAT ATCTCGATTG
    AAAGAAATTT GTGAAGTAGC ATTAACTGAA AAACTTACCC TTAAGAATGC TGCTATGCTA
    CTGGAATTTG CAGCAATGTA TAATGCTGAA CAGTTGAAAC TCTCTTGTTT ACAGTTTATT
    GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGATCTCTTG ATGTTTTAAG TGATGGTGTT
    TTGAAGGACC TTTCTGTGTT TTACCGGAAA ATGATTCCAG CAATGGAGAG AAGAGTCATT
    ACACCATATC AAGATGGACC AGATATTAGC TATTTGGATG TAGAAGATGG AGATATCTTT
    TTGAAAGAAG AAGTAAATAT GGAACAGAAT TACTCGGAAA GTATGGTCAA GAAGACAAAA
    ACAAAAGCAA AAAAGAAGCC ACGTAAACGA TCAGATAGTT CTGGAGGTTA TAATCTTTCA
    GATATTATTC AGAGTCCACC ATCTACAGGA TTGTTAAAGT CTGGTAAGAC CAATTCTGTG
    GAGTCTCTTC CAGAACTGTT GACATCAGAC TCTGAAGGAA GCTATGCAGG AGTGGGTAGT
    CCTAGAGATT TACAGTCTCC TGACTTCACA ACAGGATTTC ATTCAGATAA GTCTGAGGGG
    AAAATTAAAT CGTATGTTAA TGGTACACCT CCTGCATATT TTAGGGAAGA TTTAAAACCA
    TGGGAAAAAT CACCAGTACT TAAAATAACT GCTCCACAGC CCATTCCCAG TAACAGAATT
    GATACTACAA GCTCTTCCAA TTGGGTGTCT GGCTCTTTCA GTCCTGTCAG CCCTCCTGTC
    ATGGATCTCA GAACCATCAT GCAAATTGAA GAAAGCAGAC AAAAATGTGG AACTACACCA
    AAGACAAATT TGGGAAAAAT GATTTCTCAT GGAGTTAAAC TTTCTCAGAA GCAACGAAAA
    ATGATTGCAT TGACTACCAA AGATAATAAT TCAGGAATGA ATAACATGGA AACAGCTTTA
    AGTGCGCCTT CAAAAATCCC CAAGCCAGCG AATGCATGGG CATCTTCTCA ACATTCAGTT
    TCATCCAAGT CATTCCGGGA TTTCTTAGTA GAAGAAAAGA AATCTGTTAC TGGCCAGAGA
    TCAGGAGATC ATGTCAAAAA AGTTTGTTTT AAAGGGATTG AAAATTCCCA GTCTCCAAAA
    GTTGTAAGAT GTTCTGCTCA TGGTTCCCCA GGACGAGAAG GCAACCACAT TTCAGATGTA
    CCACTTCCGG ACAGTCCCAA TCCCTGGCTG TCTTCTTCAC TGACCGCTCC TTCCGTGGTA
    GCACCAGTCA CTTTTGCGTC TATCGTAGAA GAAGAGCTAC AGCAGGAAGC AGCTCTTATC
    AGAAGCCGAG AGAAACCCTT GGCTTTGATT CAGATTGAGG AGCGTGCAAT ACAGGATTTG
    TTGGTTTTCT ATGAAGCATT TGGAAACCCT GATGAGTTTG TCATTGTTGA AAGGACACCA
    CAGGGACCAC TGGCAGTACC TATGTGGAAT AAGCATGGCT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210753.1
    CDS352..4596
    Translation

    Target ORF information:

    RefSeq Version XM_005210696.3
    Organism Bos taurus(cattle)
    Definition Bos taurus inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210696.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCAAAAT ACATTGTACA AATCTATATA GTTTTATACT GTTGCCAGGA GGATTTAAAT 
    TATAACCCTA AGCAGGCCGT TTATTCTCTA TCAGTCTTTC TGAATAGCAG CTTTGTGGCC
    TGGCTTTTTC ATTTTTTAAA ATTATCTGAC TATTCTGAAG AAGGAAGCTG CATAAAAACA
    AATAGGATGA ATTCCTCCAT CCCTGACTGC ACATCAAAGT GTCGCTCCCT GACGCATGCT
    TTGGATGTCC TTTCTGTGGT AACGAAGGGG AGTGAAAACC AGATTAAGGC CTTTCTATCC
    AGTCATTGTC ACAATGCTGC AACCATCAGG GATGCCTTTG GTCGGAATGC CCTCCACCTT
    GTTGCCTCCT GTGGGAAAAA AGGAGTGTTA GATTGGCTTA TTGAGAAAGG AGTGGATCCC
    TCGGTGAAAG ACAAGGAGTC AGGATGGACA GCTTTGCATA GAAGCATTTT TTATGGGCAT
    ATTGATTGTG TTTGGTCTCT ACTGAAGCAT GGTGTTAATC TGTATATTCA AGATAAAGAA
    GGCTTGTCAG CTTTGGATCT TGTAATGAAG GATAGACCAA CTCATGTAGT ATTCAAAAAT
    ACTGATCCTA CAGATGTCTA CACTTGGGGA GATAATACAA ATTTCACCCT GGGTCATGGA
    AGCCAGAATA GCAAACATCA TCCAGAGTTG GTGGATCTGT TCTCCAGGAG TGGGATTTAT
    ATCAAGCAGG TGGTGCTTTG TAAATTTCAC TCAGTGTTTC TGTCTCAGAA AGGGCAGGTT
    TACACCTGTG GTCATGGTCC TGGAGGACGA TTGGGCCATG GAGATGAGCA GACATGCTTG
    GTCCCTAGGC TTGTGGAAGG ACTGAGTGGT CATAATTGTT CTCAAGTAGC GGCTGCTAAG
    GATCATACTG TTGTATTAAC TGAAGATGGA TGTGTTTATA CATTTGGTCT AAATATTTTT
    CATCAATTAG GACTCATTCC TCCGCCTTCC AGTTGTAATG TACCCAGACA GATGCAGGCA
    AAGTACCTGA AAGGAAGGAC AATCATTGGA GTAGCAGCTG GCAGGTTTCA TACTGTGCTG
    TGGACTAGAG AGGCTGTTTA CACTATGGGA CTAAATGGTG GACAATTGGG TTATTTACTA
    GATCCCAATG GAGAAAAGTG TGTAACTGCT CCTCGTCAAG TCTCTGCCCT TCATCATAAG
    GACATCACTC TGTCTTTGGT TGCTGCTAGT GATGGGGCTA CTGTCTGTGT TACCACAAAG
    GGAGATATTT ACTTACTTGC AGACTATCAG TGCAAGAAGC TGGCTTCTAA ACAACTGAAC
    TTGAAAAAGG TTCTTGTATC TGGGGGGCAT ATGGAATACA AAGTTGATCC TGAACATTTG
    AAAGAAAATG GGGGTCAGAA AATTTGCATT CTTGCAATGG ATGGAGCTGG AAGGGTATTT
    TGCTGGAGAT CTGTCAACAG TTCTATGAAG CAGTGTCGAT GGGCTTATCC ACGCCAAGTC
    TTCATTTCTG ATATTGCTTT AAGTAGAAAT GAAATTCTTT TTGTCACACA AGATGGAGAG
    GGATTTAGAG GGAAATGGTT TGAAGAAAAA AGAAAGAGTT CTGAAAAGAA AGAGATTTTA
    TCAAACCTTC ATAATTCCTC ACCAGATGTG TCTTGTGTCT CTGATATAAA TAGTGTATAT
    GAAAGAATTC GACTTGAGAA ACTTACTTTT GCCCATAGAG CTGTTAGTGT CAGCACAGAT
    CCAAGTGGAT GCAACTTTGC AGTCTTGCAG TCAGATCCTA AAACAAGCCT TTATGAAATT
    CCAACTGTGT CCTCCTCATC CTTTTTTGAG GAGTTTGGCA AGCTGTTGAG GGAAACGGAT
    GAAATGGACA ACATCCATGA TGTGACATTT CAAATTGGCA GTAGACTCTT CCCTGCACAT
    AAATATATTT TGGCAGTGCG TTCTGACTTT TTCCAGAAAT TGTTTCTTTC GGATGGTACC
    GCATTAGACT TCACAGATGT TTACCGGAAA GATGAAGATT CTACAGGCTG CCATCTCTTT
    GTGGTAGAGA AGGTTCATCC TGACCTGTTT GAATACCTTT TGCAGTTTAT ATACACAGAT
    ACTTGTGACT TTTTAACTCA TGGCTTCAAA CCAAGACTAA ATTTAAACAG AAAACCAGAA
    GGATATCCAA GCACTCCAAA CTGTCATTCG AATAAAATGA ATTCCCATGA AGATAATCAG
    AAGTCAGCAT TTGAAGCTTA CAAAAGTAAT CAAGCTCATA CAATTAGTGA AAAGCAGAAG
    AGTAAATCCA AATCTTCCAA AAAAGTAAAA AGTGTTGGGG AAGATGATCC TGTAAAAATG
    TTGCAAAATG TCGCAAAGAA ATTTGGTTTC AGTAATTTAA GTAGTAGGTT AGATGGAGTC
    AGATATGAAA ATGAAGAAAT TAGTATCATT GCCAAGAAAA CTGGTAGTAA ACCAAAGCTA
    AATCAGAAAA AATGTTCATT TTTATGTGAT GTGACCATGA AATCAGTGGA TGGAAAGGAA
    TTTCCTTGTC ATAAATGTGT TCTTTGTGCT AGACTTGAAT ATTTTCATAG TATGCTGAGT
    AGCTCATGGA TTGAGGCTTC CAGTTGTGCA GCTCTGGAAA TGCCAATACA TTCTGACATA
    CTGAAAGTTA TTTTGGACTA CCTCTATACT GATGAAGCTG TTGTGATAAA AGAATCTCAA
    AACGTGGATT TTGTTTGTAG TGTTCTTGTG GTGGCTGATC AGCTTCTCAT ATCTCGATTG
    AAAGAAATTT GTGAAGTAGC ATTAACTGAA AAACTTACCC TTAAGAATGC TGCTATGCTA
    CTGGAATTTG CAGCAATGTA TAATGCTGAA CAGTTGAAAC TCTCTTGTTT ACAGTTTATT
    GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGATCTCTTG ATGTTTTAAG TGATGGTGTT
    TTGAAGGACC TTTCTGTGTT TTACCGGAAA ATGATTCCAG CAATGGAGAG AAGAGTCATT
    ACACCATATC AAGATGGACC AGATATTAGC TATTTGGATG TAGAAGATGG AGATATCTTT
    TTGAAAGAAG AAGTAAATAT GGAACAGAAT TACTCGGAAA GTATGGTCAA GAAGACAAAA
    ACAAAAGCAA AAAAGAAGCC ACGTAAACGA TCAGATAGTT CTGGAGGTTA TAATCTTTCA
    GATATTATTC AGAGTCCACC ATCTACAGGA TTGTTAAAGT CTGGTAAGAC CAATTCTGTG
    GAGTCTCTTC CAGAACTGTT GACATCAGAC TCTGAAGGAA GCTATGCAGG AGTGGGTAGT
    CCTAGAGATT TACAGTCTCC TGACTTCACA ACAGGATTTC ATTCAGATAA GTCTGAGGGG
    AAAATTAAAT CGTATGTTAA TGGTACACCT CCTGCATATT TTAGGGAAGA TTTAAAACCA
    TGGGAAAAAT CACCAGTACT TAAAATAACT GCTCCACAGC CCATTCCCAG TAACAGAATT
    GATACTACAA GCTCTTCCAA TTGGGTGTCT GGCTCTTTCA GTCCTGTCAG CCCTCCTGTC
    ATGGATCTCA GAACCATCAT GCAAATTGAA GAAAGCAGAC AAAAATGTGG AACTACACCA
    AAGACAAATT TGGGAAAAAT GATTTCTCAT GGAGTTAAAC TTTCTCAGAA GCAACGAAAA
    ATGATTGCAT TGACTACCAA AGATAATAAT TCAGGAATGA ATAACATGGA AACAGCTTTA
    AGTGCGCCTT CAAAAATCCC CAAGCCAGCG AATGCATGGG CATCTTCTCA ACATTCAGTT
    TCATCCAAGT CATTCCGGGA TTTCTTAGTA GAAGAAAAGA AATCTGTTAC TGGCCAGAGA
    TCAGGAGATC ATGTCAAAAA AGTTTGTTTT AAAGGGATTG AAAATTCCCA GTCTCCAAAA
    GTTGTAAGAT GTTCTGCTCA TGGTTCCCCA GGACGAGAAG GCAACCACAT TTCAGATGTA
    CCACTTCCGG ACAGTCCCAA TCCCTGGCTG TCTTCTTCAC TGACCGCTCC TTCCGTGGTA
    GCACCAGTCA CTTTTGCGTC TATCGTAGAA GAAGAGCTAC AGCAGGAAGC AGCTCTTATC
    AGAAGCCGAG AGAAACCCTT GGCTTTGATT CAGATTGAGG AGCGTGCAAT ACAGGATTTG
    TTGGTTTTCT ATGAAGCATT TGGAAACCCT GATGAGTTTG TCATTGTTGA AAGGACACCA
    CAGGGACCAC TGGCAGTACC TATGTGGAAT AAGCATGGCT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.