SPAG9 cDNA ORF clone, Bos taurus(cattle)

The following SPAG9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAG9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa226504 XM_005220611.4
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa226505 XM_005220615.4
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa226506 XM_024980368.1
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa226507 XM_024980369.1
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa226508 XM_024980370.1
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa226509 XM_005220620.4
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa226510 XM_024980372.1
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa226510 XM_024980371.1
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa39848 NM_001076925.1
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa29946 XM_005220611.3
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa29950 XM_005220615.3
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa29951 XM_005220616.3
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa29955 XM_005220620.3
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa54790 XM_010816157.2
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa54791 XM_010816158.2
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa54792 XM_010816159.2
Latest version!
Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa226504
    Clone ID Related Accession (Same CDS sequence) XM_005220611.4
    Accession Version XM_005220611.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220611.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACTACTA AGAAGCCCGA ACCACCAGTT
    AATCTGAAGT ACAATGCACC AACCTCTCAC GTTACGCCTT CCGTCAAGAA AAGAAGCAGC
    ACCTTATCTC AACTCCCTGG GGATAAGTCC AGAGCCTTTG ATTTCCTTAG CGAAGAAACT
    GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAAC AGTATCGTCA GGTAAAGGCC
    CATGTTCAGA AGGAAGATGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAATAC
    AAACAGGTAA CCAACGGACA AGGGGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT
    CTCAGACCTC TAGATGAAAA AGATGCATCA ATGAAGCTTT GGTGTGCTGT TGGAGTCAAT
    TTGTCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT GTTTTACAAG
    GATGTTGCTG GTTTGGAAGC AGAAGGCAGT AAACAGCGAA GTGCATCTCA GAATAGCTTA
    GACAAGTTAG ACCAGGAACT TAAGGAACAA CAGAAGGAGT TAAAAAATCA AGAAGAATTA
    TCCAGTCTAG TCTGGATCTG TACCAGCACT CATTCAGCTA CAAAAGTTAT CATTATCGAT
    GCTGTTCAAC CAGGCAACAT CCTAGACAGC TTCACTGTTT GTAACTCTCA CGTTCTGTGT
    ATATCAAGCG TACCAGGAGC CCGAGAAACA GACTACCCTG CAGGAGAAGA GCTTTCAGAA
    TCTGGACAAG TAGACAAAGC ATCTTTATGT GGAAGCATGA CAAGCAATAG CTCAGCAGAG
    ACAGACAGCC TGTTGGGAGG CATCACAGTA GTTGGTTGTT CTGCAGAAGG TGTGACAGCA
    GCTGCCACTT CCCCTAATAC CAATGAACCT TCTCCAGTGA TGGAAAAACC GCCAGAAAAG
    GAAACAGAAA ATAGTGAGAT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT
    ACAGAAGGCA ATGCAGGATC AGCTGAAGAT ACTGTGGATG TCTCCCAAAC TGGCGTGTAC
    ACAGAGCATG TGTTTACAGA TCCTTTGGGA GTTCAAATCC CAGAAGACCT GTCACCAGTA
    TATCAGTCAA GTAATGACTC AGATGCATAT AAAGATCAAA TATCAGTATT GCCAAATGAA
    CAAGACCTGG TGAGAGAGGA AGCCCAGAAA ATGAGTAGCC TTTTACCAAC TATGTGGCTT
    GGAGCTCAAA ATGGGTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC
    CATTCTATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAAGG AATTGTGTTA
    GTGGCCCTAG CTGATGGGAC CCTTGCCATC TTTCACAGAG GAGTTGATGG ACAGTGGGAT
    TTGTCAAACT ATCACCTGTT AGATCTTGGA CGACCGCACC ATTCCATCCG ATGCATGACT
    GTGGTACATG ACAAAGTTTG GTGTGGCTAT AGGAACAAAA TTTATGTGGT TCAGCCAAAG
    GCCATGAAAA TAGAGAAATC ATTTGATGCA CACCCCAGGA AGGAGAGCCA AGTGCGGCAG
    CTTGCCTGGG TGGGTGATGG CGTGTGGGTC TCCATTCGCT TGGATTCCAC ACTCCGTCTC
    TATCATGCAC ACACTTATCA GCATCTGCAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA
    ATGCTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGGA TCACAGCGCT TATGGTGTCT
    TGTAACCGTT TGTGGGTGGG AACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA
    ACCGTAATCC TCCACCAGGG ACGTTTACTG GGGCTGAGGG CGAATAAAAC ATCAGGAGCA
    TCGGGAAATC GTCCTGGAAG TGTCATTCGA GTATATGGTG ATGAAAACAG TGATAAAGTG
    ACACCAGGGA CATTTATACC CTATTGTTCA ATGGCACATG CACAGCTTTG CTTCCATGGG
    CACAGGGATG CTGTGAAATT TTTTGTGGCT GTCCCAGGTC AAGTCATCAG CCCACAAAGT
    GGCAGCAGTG GTACAGATCT GACAGTTGAC AAAGCAGGGC CATCTGCACA GGAGCCTGGC
    ATCCAGACAC CCTTGAAGTC TGTGCTTGTC ATTAGTGGAG GAGAGGGCTA CATTGACTTC
    CGAATGGGTG ATGAAGGTGG AGAATCAGAG CTTCTTGGAG AGGATCTTCC ACTTGAACCT
    TCTGTCGCCA AAGCAGAAAG GAGTCACTTG ATAGTGTGGC AAGTGATGTA TGGTAGTGAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220668.1
    CDS157..4119
    Translation

    Target ORF information:

    RefSeq Version XM_005220611.4
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220611.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACTACTA AGAAGCCCGA ACCACCAGTT
    AATCTGAAGT ACAATGCACC AACCTCTCAC GTTACGCCTT CCGTCAAGAA AAGAAGCAGC
    ACCTTATCTC AACTCCCTGG GGATAAGTCC AGAGCCTTTG ATTTCCTTAG CGAAGAAACT
    GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAAC AGTATCGTCA GGTAAAGGCC
    CATGTTCAGA AGGAAGATGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAATAC
    AAACAGGTAA CCAACGGACA AGGGGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT
    CTCAGACCTC TAGATGAAAA AGATGCATCA ATGAAGCTTT GGTGTGCTGT TGGAGTCAAT
    TTGTCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT GTTTTACAAG
    GATGTTGCTG GTTTGGAAGC AGAAGGCAGT AAACAGCGAA GTGCATCTCA GAATAGCTTA
    GACAAGTTAG ACCAGGAACT TAAGGAACAA CAGAAGGAGT TAAAAAATCA AGAAGAATTA
    TCCAGTCTAG TCTGGATCTG TACCAGCACT CATTCAGCTA CAAAAGTTAT CATTATCGAT
    GCTGTTCAAC CAGGCAACAT CCTAGACAGC TTCACTGTTT GTAACTCTCA CGTTCTGTGT
    ATATCAAGCG TACCAGGAGC CCGAGAAACA GACTACCCTG CAGGAGAAGA GCTTTCAGAA
    TCTGGACAAG TAGACAAAGC ATCTTTATGT GGAAGCATGA CAAGCAATAG CTCAGCAGAG
    ACAGACAGCC TGTTGGGAGG CATCACAGTA GTTGGTTGTT CTGCAGAAGG TGTGACAGCA
    GCTGCCACTT CCCCTAATAC CAATGAACCT TCTCCAGTGA TGGAAAAACC GCCAGAAAAG
    GAAACAGAAA ATAGTGAGAT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT
    ACAGAAGGCA ATGCAGGATC AGCTGAAGAT ACTGTGGATG TCTCCCAAAC TGGCGTGTAC
    ACAGAGCATG TGTTTACAGA TCCTTTGGGA GTTCAAATCC CAGAAGACCT GTCACCAGTA
    TATCAGTCAA GTAATGACTC AGATGCATAT AAAGATCAAA TATCAGTATT GCCAAATGAA
    CAAGACCTGG TGAGAGAGGA AGCCCAGAAA ATGAGTAGCC TTTTACCAAC TATGTGGCTT
    GGAGCTCAAA ATGGGTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC
    CATTCTATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAAGG AATTGTGTTA
    GTGGCCCTAG CTGATGGGAC CCTTGCCATC TTTCACAGAG GAGTTGATGG ACAGTGGGAT
    TTGTCAAACT ATCACCTGTT AGATCTTGGA CGACCGCACC ATTCCATCCG ATGCATGACT
    GTGGTACATG ACAAAGTTTG GTGTGGCTAT AGGAACAAAA TTTATGTGGT TCAGCCAAAG
    GCCATGAAAA TAGAGAAATC ATTTGATGCA CACCCCAGGA AGGAGAGCCA AGTGCGGCAG
    CTTGCCTGGG TGGGTGATGG CGTGTGGGTC TCCATTCGCT TGGATTCCAC ACTCCGTCTC
    TATCATGCAC ACACTTATCA GCATCTGCAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA
    ATGCTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGGA TCACAGCGCT TATGGTGTCT
    TGTAACCGTT TGTGGGTGGG AACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA
    ACCGTAATCC TCCACCAGGG ACGTTTACTG GGGCTGAGGG CGAATAAAAC ATCAGGAGCA
    TCGGGAAATC GTCCTGGAAG TGTCATTCGA GTATATGGTG ATGAAAACAG TGATAAAGTG
    ACACCAGGGA CATTTATACC CTATTGTTCA ATGGCACATG CACAGCTTTG CTTCCATGGG
    CACAGGGATG CTGTGAAATT TTTTGTGGCT GTCCCAGGTC AAGTCATCAG CCCACAAAGT
    GGCAGCAGTG GTACAGATCT GACAGTTGAC AAAGCAGGGC CATCTGCACA GGAGCCTGGC
    ATCCAGACAC CCTTGAAGTC TGTGCTTGTC ATTAGTGGAG GAGAGGGCTA CATTGACTTC
    CGAATGGGTG ATGAAGGTGG AGAATCAGAG CTTCTTGGAG AGGATCTTCC ACTTGAACCT
    TCTGTCGCCA AAGCAGAAAG GAGTCACTTG ATAGTGTGGC AAGTGATGTA TGGTAGTGAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226505
    Clone ID Related Accession (Same CDS sequence) XM_005220615.4
    Accession Version XM_005220615.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220615.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACTACTA AGAAGCCCGA ACCACCAGTT
    AATCTGAAGT ACAATGCACC AACCTCTCAC GTTACGCCTT CCGTCAAGAA AAGAAGCAGC
    ACCTTATCTC AACTCCCTGG GGATAAGTCC AGAGCCTTTG ATTTCCTTAG CGAAGAAACT
    GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAAC AGTATCGTCA GGTAAAGGCC
    CATGTTCAGA AGGAAGATGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAATAC
    AAACAGGTAA CCAACGGACA AGGGGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT
    CTCAGACCTC TAGATGAAAA AGATGCATCA ATGAAGCTTT GGTGTGCTGT TGGAGTCAAT
    TTGTCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT GTTTTACAAG
    GATGTTGCTG GTTTGGAAGC AGAAGGCAGT AAACAGCGAA GTGCATCTCA GAATAGCTTA
    GACAAGTTAG ACCAGGAACT TAAGGAACAA CAGAAGGAGT TAAAAAATCA AGAAGAATTA
    TCCAGTCTAG TCTGGATCTG TACCAGCACT CATTCAGCTA CAAAAGTTAT CATTATCGAT
    GCTGTTCAAC CAGGCAACAT CCTAGACAGC TTCACTGTTT GTAACTCTCA CGTTCTGTGT
    ATATCAAGCG TACCAGGAGC CCGAGAAACA GACTACCCTG CAGGAGAAGA GCTTTCAGAA
    TCTGGACAAG TAGACAAAGC ATCTTTATGT GGAAGCATGA CAAGCAATAG CTCAGCAGAG
    ACAGACAGCC TGTTGGGAGG CATCACAGTA GTTGGTTGTT CTGCAGAAGG TGTGACAGCA
    GCTGCCACTT CCCCTAATAC CAATGAACCT TCTCCAGTGA TGGAAAAACC GCCAGAAAAG
    GAAACAGAAA ATAGTGAGAT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT
    ACAGAAGGCA ATGCAGGATC AGCTGAAGAT ACTGTGGATG TCTCCCAAAC TGGCGTGTAC
    ACAGAGCATG TGTTTACAGA TCCTTTGGGA GTTCAAATCC CAGAAGACCT GTCACCAGTA
    TATCAGTCAA GTAATGACTC AGATGCATAT AAAGATCAAA TATCAGTATT GCCAAATGAA
    CAAGACCTGG TGAGAGAGGA AGCCCAGAAA ATGAGTAGCC TTTTACCAAC TATGTGGCTT
    GGAGCTCAAA ATGGGTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC
    CATTCTATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAAGG AATTGTGTTA
    GTGGCCCTAG CTGATGGGAC CCTTGCCATC TTTCACAGAG GAGTTGATGG ACAGTGGGAT
    TTGTCAAACT ATCACCTGTT AGATCTTGGA CGACCGCACC ATTCCATCCG ATGCATGACT
    GTGGTACATG ACAAAGTTTG GTGTGGCTAT AGGAACAAAA TTTATGTGGT TCAGCCAAAG
    GCCATGAAAA TAGAGAAATC ATTTGATGCA CACCCCAGGA AGGAGAGCCA AGTGCGGCAG
    CTTGCCTGGG TGGGTGATGG CGTGTGGGTC TCCATTCGCT TGGATTCCAC ACTCCGTCTC
    TATCATGCAC ACACTTATCA GCATCTGCAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA
    ATGCTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGGA TCACAGCGCT TATGGTGTCT
    TGTAACCGTT TGTGGGTGGG AACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA
    ACGAATAAAA CATCAGGAGC ATCGGGAAAT CGTCCTGGAA GTGTCATTCG AGTATATGGT
    GATGAAAACA GTGATAAAGT GACACCAGGG ACATTTATAC CCTATTGTTC AATGGCACAT
    GCACAGCTTT GCTTCCATGG GCACAGGGAT GCTGTGAAAT TTTTTGTGGC TGTCCCAGGT
    CAAGTCATCA GCCCACAAAG TGGCAGCAGT GGTACAGATC TGACAGTTGA CAAAGCAGGG
    CCATCTGCAC AGGAGCCTGG CATCCAGACA CCCTTGAAGT CTGTGCTTGT CATTAGTGGA
    GGAGAGGGCT ACATTGACTT CCGAATGGGT GATGAAGGTG GAGAATCAGA GCTTCTTGGA
    GAGGATCTTC CACTTGAACC TTCTGTCGCC AAAGCAGAAA GGAGTCACTT GATAGTGTGG
    CAAGTGATGT ATGGTAGTGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220672.1
    CDS191..4114
    Translation

    Target ORF information:

    RefSeq Version XM_005220615.4
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220615.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACTACTA AGAAGCCCGA ACCACCAGTT
    AATCTGAAGT ACAATGCACC AACCTCTCAC GTTACGCCTT CCGTCAAGAA AAGAAGCAGC
    ACCTTATCTC AACTCCCTGG GGATAAGTCC AGAGCCTTTG ATTTCCTTAG CGAAGAAACT
    GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAAC AGTATCGTCA GGTAAAGGCC
    CATGTTCAGA AGGAAGATGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAATAC
    AAACAGGTAA CCAACGGACA AGGGGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT
    CTCAGACCTC TAGATGAAAA AGATGCATCA ATGAAGCTTT GGTGTGCTGT TGGAGTCAAT
    TTGTCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT GTTTTACAAG
    GATGTTGCTG GTTTGGAAGC AGAAGGCAGT AAACAGCGAA GTGCATCTCA GAATAGCTTA
    GACAAGTTAG ACCAGGAACT TAAGGAACAA CAGAAGGAGT TAAAAAATCA AGAAGAATTA
    TCCAGTCTAG TCTGGATCTG TACCAGCACT CATTCAGCTA CAAAAGTTAT CATTATCGAT
    GCTGTTCAAC CAGGCAACAT CCTAGACAGC TTCACTGTTT GTAACTCTCA CGTTCTGTGT
    ATATCAAGCG TACCAGGAGC CCGAGAAACA GACTACCCTG CAGGAGAAGA GCTTTCAGAA
    TCTGGACAAG TAGACAAAGC ATCTTTATGT GGAAGCATGA CAAGCAATAG CTCAGCAGAG
    ACAGACAGCC TGTTGGGAGG CATCACAGTA GTTGGTTGTT CTGCAGAAGG TGTGACAGCA
    GCTGCCACTT CCCCTAATAC CAATGAACCT TCTCCAGTGA TGGAAAAACC GCCAGAAAAG
    GAAACAGAAA ATAGTGAGAT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT
    ACAGAAGGCA ATGCAGGATC AGCTGAAGAT ACTGTGGATG TCTCCCAAAC TGGCGTGTAC
    ACAGAGCATG TGTTTACAGA TCCTTTGGGA GTTCAAATCC CAGAAGACCT GTCACCAGTA
    TATCAGTCAA GTAATGACTC AGATGCATAT AAAGATCAAA TATCAGTATT GCCAAATGAA
    CAAGACCTGG TGAGAGAGGA AGCCCAGAAA ATGAGTAGCC TTTTACCAAC TATGTGGCTT
    GGAGCTCAAA ATGGGTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC
    CATTCTATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAAGG AATTGTGTTA
    GTGGCCCTAG CTGATGGGAC CCTTGCCATC TTTCACAGAG GAGTTGATGG ACAGTGGGAT
    TTGTCAAACT ATCACCTGTT AGATCTTGGA CGACCGCACC ATTCCATCCG ATGCATGACT
    GTGGTACATG ACAAAGTTTG GTGTGGCTAT AGGAACAAAA TTTATGTGGT TCAGCCAAAG
    GCCATGAAAA TAGAGAAATC ATTTGATGCA CACCCCAGGA AGGAGAGCCA AGTGCGGCAG
    CTTGCCTGGG TGGGTGATGG CGTGTGGGTC TCCATTCGCT TGGATTCCAC ACTCCGTCTC
    TATCATGCAC ACACTTATCA GCATCTGCAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA
    ATGCTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGGA TCACAGCGCT TATGGTGTCT
    TGTAACCGTT TGTGGGTGGG AACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA
    ACGAATAAAA CATCAGGAGC ATCGGGAAAT CGTCCTGGAA GTGTCATTCG AGTATATGGT
    GATGAAAACA GTGATAAAGT GACACCAGGG ACATTTATAC CCTATTGTTC AATGGCACAT
    GCACAGCTTT GCTTCCATGG GCACAGGGAT GCTGTGAAAT TTTTTGTGGC TGTCCCAGGT
    CAAGTCATCA GCCCACAAAG TGGCAGCAGT GGTACAGATC TGACAGTTGA CAAAGCAGGG
    CCATCTGCAC AGGAGCCTGG CATCCAGACA CCCTTGAAGT CTGTGCTTGT CATTAGTGGA
    GGAGAGGGCT ACATTGACTT CCGAATGGGT GATGAAGGTG GAGAATCAGA GCTTCTTGGA
    GAGGATCTTC CACTTGAACC TTCTGTCGCC AAAGCAGAAA GGAGTCACTT GATAGTGTGG
    CAAGTGATGT ATGGTAGTGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226506
    Clone ID Related Accession (Same CDS sequence) XM_024980368.1
    Accession Version XM_024980368.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACTACTA AGAAGCCCGA ACCACCAGTT
    AATCTGAAGT ACAATGCACC AACCTCTCAC GTTACGCCTT CCGTCAAGAA AAGAAGCAGC
    ACCTTATCTC AACTCCCTGG GGATAAGTCC AGAGCCTTTG ATTTCCTTAG CGAAGAAACT
    GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAAC AGTATCGTCA GGTAAAGGCC
    CATGTTCAGA AGGAAGATGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAATAC
    AAACAGGTAA CCAACGGACA AGGGGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT
    CTCAGACCTC TAGATGAAAA AGATGCATCA ATGAAGCTTT GGTGTGCTGT TGGAGTCAAT
    TTGTCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT GTTTTACAAG
    GATGTTGCTG GTTTGGAAGC AGAAGGCAGT AAACAGCGAA GTGCATCTCA GAATAGCTTA
    GACAAGTTAG ACCAGGAACT TAAGGAACAA CAGAAGGAGT TAAAAAATCA AGAAGAATTA
    TCCAGTCTAG TCTGGATCTG TACCAGCACT CATTCAGCTA CAAAAGTTAT CATTATCGAT
    GCTGTTCAAC CAGGCAACAT CCTAGACAGC TTCACTGTTT GTAACTCTCA CGTTCTGTGT
    ATATCAAGCG TACCAGGAGC CCGAGAAACA GACTACCCTG CAGGAGAAGA GCTTTCAGAA
    TCTGGACAAG TAGACAAAGC ATCTTTATGT GGAAGCATGA CAAGCAATAG CTCAGCAGAG
    ACAGACAGCC TGTTGGGAGG CATCACAGTA GTTGGTTGTT CTGCAGAAGG TGTGACAGCA
    GCTGCCACTT CCCCTAATAC CAATGAACCT TCTCCAGTGA TGGAAAAACC GCCAGCAGAA
    GAAGCAACTG AAGCTACAGA AGGCAATGCA GGATCAGCTG AAGATACTGT GGATGTCTCC
    CAAACTGGCG TGTACACAGA GCATGTGTTT ACAGATCCTT TGGGAGTTCA AATCCCAGAA
    GACCTGTCAC CAGTATATCA GTCAAGTAAT GACTCAGATG CATATAAAGA TCAAATATCA
    GTATTGCCAA ATGAACAAGA CCTGGTGAGA GAGGAAGCCC AGAAAATGAG TAGCCTTTTA
    CCAACTATGT GGCTTGGAGC TCAAAATGGG TGTTTGTATG TCCATTCATC TGTAGCCCAG
    TGGAGGAAAT GTCTCCATTC TATTAAACTT AAAGATTCGA TTCTCAGTAT TGTACACGTG
    AAAGGAATTG TGTTAGTGGC CCTAGCTGAT GGGACCCTTG CCATCTTTCA CAGAGGAGTT
    GATGGACAGT GGGATTTGTC AAACTATCAC CTGTTAGATC TTGGACGACC GCACCATTCC
    ATCCGATGCA TGACTGTGGT ACATGACAAA GTTTGGTGTG GCTATAGGAA CAAAATTTAT
    GTGGTTCAGC CAAAGGCCAT GAAAATAGAG AAATCATTTG ATGCACACCC CAGGAAGGAG
    AGCCAAGTGC GGCAGCTTGC CTGGGTGGGT GATGGCGTGT GGGTCTCCAT TCGCTTGGAT
    TCCACACTCC GTCTCTATCA TGCACACACT TATCAGCATC TGCAGGATGT GGACATTGAG
    CCTTATGTAA GCAAAATGCT AGGTACTGGA AAACTGGGCT TCTCTTTTGT GAGGATCACA
    GCGCTTATGG TGTCTTGTAA CCGTTTGTGG GTGGGAACAG GAAATGGTGT CATTATCTCC
    ATCCCATTGA CAGAAACCGT AATCCTCCAC CAGGGACGTT TACTGGGGCT GAGGGCGAAT
    AAAACATCAG GAGCATCGGG AAATCGTCCT GGAAGTGTCA TTCGAGTATA TGGTGATGAA
    AACAGTGATA AAGTGACACC AGGGACATTT ATACCCTATT GTTCAATGGC ACATGCACAG
    CTTTGCTTCC ATGGGCACAG GGATGCTGTG AAATTTTTTG TGGCTGTCCC AGGTCAAGTC
    ATCAGCCCAC AAAGTGGCAG CAGTGGTACA GATCTGACAG TTGACAAAGC AGGGCCATCT
    GCACAGGAGC CTGGCATCCA GACACCCTTG AAGTCTGTGC TTGTCATTAG TGGAGGAGAG
    GGCTACATTG ACTTCCGAAT GGGTGATGAA GGTGGAGAAT CAGAGCTTCT TGGAGAGGAT
    CTTCCACTTG AACCTTCTGT CGCCAAAGCA GAAAGGAGTC ACTTGATAGT GTGGCAAGTG
    ATGTATGGTA GTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836136.1
    CDS87..4004
    Translation

    Target ORF information:

    RefSeq Version XM_024980368.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980368.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACTACTA AGAAGCCCGA ACCACCAGTT
    AATCTGAAGT ACAATGCACC AACCTCTCAC GTTACGCCTT CCGTCAAGAA AAGAAGCAGC
    ACCTTATCTC AACTCCCTGG GGATAAGTCC AGAGCCTTTG ATTTCCTTAG CGAAGAAACT
    GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAAC AGTATCGTCA GGTAAAGGCC
    CATGTTCAGA AGGAAGATGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAATAC
    AAACAGGTAA CCAACGGACA AGGGGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT
    CTCAGACCTC TAGATGAAAA AGATGCATCA ATGAAGCTTT GGTGTGCTGT TGGAGTCAAT
    TTGTCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT GTTTTACAAG
    GATGTTGCTG GTTTGGAAGC AGAAGGCAGT AAACAGCGAA GTGCATCTCA GAATAGCTTA
    GACAAGTTAG ACCAGGAACT TAAGGAACAA CAGAAGGAGT TAAAAAATCA AGAAGAATTA
    TCCAGTCTAG TCTGGATCTG TACCAGCACT CATTCAGCTA CAAAAGTTAT CATTATCGAT
    GCTGTTCAAC CAGGCAACAT CCTAGACAGC TTCACTGTTT GTAACTCTCA CGTTCTGTGT
    ATATCAAGCG TACCAGGAGC CCGAGAAACA GACTACCCTG CAGGAGAAGA GCTTTCAGAA
    TCTGGACAAG TAGACAAAGC ATCTTTATGT GGAAGCATGA CAAGCAATAG CTCAGCAGAG
    ACAGACAGCC TGTTGGGAGG CATCACAGTA GTTGGTTGTT CTGCAGAAGG TGTGACAGCA
    GCTGCCACTT CCCCTAATAC CAATGAACCT TCTCCAGTGA TGGAAAAACC GCCAGCAGAA
    GAAGCAACTG AAGCTACAGA AGGCAATGCA GGATCAGCTG AAGATACTGT GGATGTCTCC
    CAAACTGGCG TGTACACAGA GCATGTGTTT ACAGATCCTT TGGGAGTTCA AATCCCAGAA
    GACCTGTCAC CAGTATATCA GTCAAGTAAT GACTCAGATG CATATAAAGA TCAAATATCA
    GTATTGCCAA ATGAACAAGA CCTGGTGAGA GAGGAAGCCC AGAAAATGAG TAGCCTTTTA
    CCAACTATGT GGCTTGGAGC TCAAAATGGG TGTTTGTATG TCCATTCATC TGTAGCCCAG
    TGGAGGAAAT GTCTCCATTC TATTAAACTT AAAGATTCGA TTCTCAGTAT TGTACACGTG
    AAAGGAATTG TGTTAGTGGC CCTAGCTGAT GGGACCCTTG CCATCTTTCA CAGAGGAGTT
    GATGGACAGT GGGATTTGTC AAACTATCAC CTGTTAGATC TTGGACGACC GCACCATTCC
    ATCCGATGCA TGACTGTGGT ACATGACAAA GTTTGGTGTG GCTATAGGAA CAAAATTTAT
    GTGGTTCAGC CAAAGGCCAT GAAAATAGAG AAATCATTTG ATGCACACCC CAGGAAGGAG
    AGCCAAGTGC GGCAGCTTGC CTGGGTGGGT GATGGCGTGT GGGTCTCCAT TCGCTTGGAT
    TCCACACTCC GTCTCTATCA TGCACACACT TATCAGCATC TGCAGGATGT GGACATTGAG
    CCTTATGTAA GCAAAATGCT AGGTACTGGA AAACTGGGCT TCTCTTTTGT GAGGATCACA
    GCGCTTATGG TGTCTTGTAA CCGTTTGTGG GTGGGAACAG GAAATGGTGT CATTATCTCC
    ATCCCATTGA CAGAAACCGT AATCCTCCAC CAGGGACGTT TACTGGGGCT GAGGGCGAAT
    AAAACATCAG GAGCATCGGG AAATCGTCCT GGAAGTGTCA TTCGAGTATA TGGTGATGAA
    AACAGTGATA AAGTGACACC AGGGACATTT ATACCCTATT GTTCAATGGC ACATGCACAG
    CTTTGCTTCC ATGGGCACAG GGATGCTGTG AAATTTTTTG TGGCTGTCCC AGGTCAAGTC
    ATCAGCCCAC AAAGTGGCAG CAGTGGTACA GATCTGACAG TTGACAAAGC AGGGCCATCT
    GCACAGGAGC CTGGCATCCA GACACCCTTG AAGTCTGTGC TTGTCATTAG TGGAGGAGAG
    GGCTACATTG ACTTCCGAAT GGGTGATGAA GGTGGAGAAT CAGAGCTTCT TGGAGAGGAT
    CTTCCACTTG AACCTTCTGT CGCCAAAGCA GAAAGGAGTC ACTTGATAGT GTGGCAAGTG
    ATGTATGGTA GTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226507
    Clone ID Related Accession (Same CDS sequence) XM_024980369.1
    Accession Version XM_024980369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3915bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACTACTA AGAAGCCCGA ACCACCAGTT
    AATCTGAAGT ACAATGCACC AACCTCTCAC GTTACGCCTT CCGTCAAGAA AAGAAGCAGC
    ACCTTATCTC AACTCCCTGG GGATAAGTCC AGAGCCTTTG ATTTCCTTAG CGAAGAAACT
    GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAAC AGTATCGTCA GGTAAAGGCC
    CATGTTCAGA AGGAAGATGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAATAC
    AAACAGGTAA CCAACGGACA AGGGGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT
    CTCAGACCTC TAGATGAAAA AGATGCATCA ATGAAGCTTT GGTGTGCTGT TGGAGTCAAT
    TTGTCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT GTTTTACAAG
    GATGTTGCTG GTTTGGAAGC AGAAGGCAGT AAACAGCGAA GTGCATCTCA GAATAGCTTA
    GACAAGTTAG ACCAGGAACT TAAGGAACAA CAGAAGGAGT TAAAAAATCA AGAAGAATTA
    TCCAGTCTAG TCTGGATCTG TACCAGCACT CATTCAGCTA CAAAAGTTAT CATTATCGAT
    GCTGTTCAAC CAGGCAACAT CCTAGACAGC TTCACTGTTT GTAACTCTCA CGTTCTGTGT
    ATATCAAGCG TACCAGGAGC CCGAGAAACA GACTACCCTG CAGGAGAAGA GCTTTCAGAA
    TCTGGACAAG TAGACAAAGC ATCTTTATGT GGAAGCATGA CAAGCAATAG CTCAGCAGAG
    ACAGACAGCC TGTTGGGAGG CATCACAGTA GTTGGTTGTT CTGCAGAAGG TGTGACAGCA
    GCTGCCACTT CCCCTAATAC CAATGAACCT TCTCCAGTGA TGGAAAAACC GCCAGAAGAA
    GCAACTGAAG CTACAGAAGG CAATGCAGGA TCAGCTGAAG ATACTGTGGA TGTCTCCCAA
    ACTGGCGTGT ACACAGAGCA TGTGTTTACA GATCCTTTGG GAGTTCAAAT CCCAGAAGAC
    CTGTCACCAG TATATCAGTC AAGTAATGAC TCAGATGCAT ATAAAGATCA AATATCAGTA
    TTGCCAAATG AACAAGACCT GGTGAGAGAG GAAGCCCAGA AAATGAGTAG CCTTTTACCA
    ACTATGTGGC TTGGAGCTCA AAATGGGTGT TTGTATGTCC ATTCATCTGT AGCCCAGTGG
    AGGAAATGTC TCCATTCTAT TAAACTTAAA GATTCGATTC TCAGTATTGT ACACGTGAAA
    GGAATTGTGT TAGTGGCCCT AGCTGATGGG ACCCTTGCCA TCTTTCACAG AGGAGTTGAT
    GGACAGTGGG ATTTGTCAAA CTATCACCTG TTAGATCTTG GACGACCGCA CCATTCCATC
    CGATGCATGA CTGTGGTACA TGACAAAGTT TGGTGTGGCT ATAGGAACAA AATTTATGTG
    GTTCAGCCAA AGGCCATGAA AATAGAGAAA TCATTTGATG CACACCCCAG GAAGGAGAGC
    CAAGTGCGGC AGCTTGCCTG GGTGGGTGAT GGCGTGTGGG TCTCCATTCG CTTGGATTCC
    ACACTCCGTC TCTATCATGC ACACACTTAT CAGCATCTGC AGGATGTGGA CATTGAGCCT
    TATGTAAGCA AAATGCTAGG TACTGGAAAA CTGGGCTTCT CTTTTGTGAG GATCACAGCG
    CTTATGGTGT CTTGTAACCG TTTGTGGGTG GGAACAGGAA ATGGTGTCAT TATCTCCATC
    CCATTGACAG AAACCGTAAT CCTCCACCAG GGACGTTTAC TGGGGCTGAG GGCGAATAAA
    ACATCAGGAG CATCGGGAAA TCGTCCTGGA AGTGTCATTC GAGTATATGG TGATGAAAAC
    AGTGATAAAG TGACACCAGG GACATTTATA CCCTATTGTT CAATGGCACA TGCACAGCTT
    TGCTTCCATG GGCACAGGGA TGCTGTGAAA TTTTTTGTGG CTGTCCCAGG TCAAGTCATC
    AGCCCACAAA GTGGCAGCAG TGGTACAGAT CTGACAGTTG ACAAAGCAGG GCCATCTGCA
    CAGGAGCCTG GCATCCAGAC ACCCTTGAAG TCTGTGCTTG TCATTAGTGG AGGAGAGGGC
    TACATTGACT TCCGAATGGG TGATGAAGGT GGAGAATCAG AGCTTCTTGG AGAGGATCTT
    CCACTTGAAC CTTCTGTCGC CAAAGCAGAA AGGAGTCACT TGATAGTGTG GCAAGTGATG
    TATGGTAGTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836137.1
    CDS87..4001
    Translation

    Target ORF information:

    RefSeq Version XM_024980369.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACTACTA AGAAGCCCGA ACCACCAGTT
    AATCTGAAGT ACAATGCACC AACCTCTCAC GTTACGCCTT CCGTCAAGAA AAGAAGCAGC
    ACCTTATCTC AACTCCCTGG GGATAAGTCC AGAGCCTTTG ATTTCCTTAG CGAAGAAACT
    GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAAC AGTATCGTCA GGTAAAGGCC
    CATGTTCAGA AGGAAGATGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAATAC
    AAACAGGTAA CCAACGGACA AGGGGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT
    CTCAGACCTC TAGATGAAAA AGATGCATCA ATGAAGCTTT GGTGTGCTGT TGGAGTCAAT
    TTGTCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT GTTTTACAAG
    GATGTTGCTG GTTTGGAAGC AGAAGGCAGT AAACAGCGAA GTGCATCTCA GAATAGCTTA
    GACAAGTTAG ACCAGGAACT TAAGGAACAA CAGAAGGAGT TAAAAAATCA AGAAGAATTA
    TCCAGTCTAG TCTGGATCTG TACCAGCACT CATTCAGCTA CAAAAGTTAT CATTATCGAT
    GCTGTTCAAC CAGGCAACAT CCTAGACAGC TTCACTGTTT GTAACTCTCA CGTTCTGTGT
    ATATCAAGCG TACCAGGAGC CCGAGAAACA GACTACCCTG CAGGAGAAGA GCTTTCAGAA
    TCTGGACAAG TAGACAAAGC ATCTTTATGT GGAAGCATGA CAAGCAATAG CTCAGCAGAG
    ACAGACAGCC TGTTGGGAGG CATCACAGTA GTTGGTTGTT CTGCAGAAGG TGTGACAGCA
    GCTGCCACTT CCCCTAATAC CAATGAACCT TCTCCAGTGA TGGAAAAACC GCCAGAAGAA
    GCAACTGAAG CTACAGAAGG CAATGCAGGA TCAGCTGAAG ATACTGTGGA TGTCTCCCAA
    ACTGGCGTGT ACACAGAGCA TGTGTTTACA GATCCTTTGG GAGTTCAAAT CCCAGAAGAC
    CTGTCACCAG TATATCAGTC AAGTAATGAC TCAGATGCAT ATAAAGATCA AATATCAGTA
    TTGCCAAATG AACAAGACCT GGTGAGAGAG GAAGCCCAGA AAATGAGTAG CCTTTTACCA
    ACTATGTGGC TTGGAGCTCA AAATGGGTGT TTGTATGTCC ATTCATCTGT AGCCCAGTGG
    AGGAAATGTC TCCATTCTAT TAAACTTAAA GATTCGATTC TCAGTATTGT ACACGTGAAA
    GGAATTGTGT TAGTGGCCCT AGCTGATGGG ACCCTTGCCA TCTTTCACAG AGGAGTTGAT
    GGACAGTGGG ATTTGTCAAA CTATCACCTG TTAGATCTTG GACGACCGCA CCATTCCATC
    CGATGCATGA CTGTGGTACA TGACAAAGTT TGGTGTGGCT ATAGGAACAA AATTTATGTG
    GTTCAGCCAA AGGCCATGAA AATAGAGAAA TCATTTGATG CACACCCCAG GAAGGAGAGC
    CAAGTGCGGC AGCTTGCCTG GGTGGGTGAT GGCGTGTGGG TCTCCATTCG CTTGGATTCC
    ACACTCCGTC TCTATCATGC ACACACTTAT CAGCATCTGC AGGATGTGGA CATTGAGCCT
    TATGTAAGCA AAATGCTAGG TACTGGAAAA CTGGGCTTCT CTTTTGTGAG GATCACAGCG
    CTTATGGTGT CTTGTAACCG TTTGTGGGTG GGAACAGGAA ATGGTGTCAT TATCTCCATC
    CCATTGACAG AAACCGTAAT CCTCCACCAG GGACGTTTAC TGGGGCTGAG GGCGAATAAA
    ACATCAGGAG CATCGGGAAA TCGTCCTGGA AGTGTCATTC GAGTATATGG TGATGAAAAC
    AGTGATAAAG TGACACCAGG GACATTTATA CCCTATTGTT CAATGGCACA TGCACAGCTT
    TGCTTCCATG GGCACAGGGA TGCTGTGAAA TTTTTTGTGG CTGTCCCAGG TCAAGTCATC
    AGCCCACAAA GTGGCAGCAG TGGTACAGAT CTGACAGTTG ACAAAGCAGG GCCATCTGCA
    CAGGAGCCTG GCATCCAGAC ACCCTTGAAG TCTGTGCTTG TCATTAGTGG AGGAGAGGGC
    TACATTGACT TCCGAATGGG TGATGAAGGT GGAGAATCAG AGCTTCTTGG AGAGGATCTT
    CCACTTGAAC CTTCTGTCGC CAAAGCAGAA AGGAGTCACT TGATAGTGTG GCAAGTGATG
    TATGGTAGTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226508
    Clone ID Related Accession (Same CDS sequence) XM_024980370.1
    Accession Version XM_024980370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3879bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACTACTA AGAAGCCCGA ACCACCAGTT
    AATCTGAAGT ACAATGCACC AACCTCTCAC GTTACGCCTT CCGTCAAGAA AAGAAGCAGC
    ACCTTATCTC AACTCCCTGG GGATAAGTCC AGAGCCTTTG ATTTCCTTAG CGAAGAAACT
    GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAAC AGTATCGTCA GGTAAAGGCC
    CATGTTCAGA AGGAAGATGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAATAC
    AAACAGGTAA CCAACGGACA AGGGGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT
    CTCAGACCTC TAGATGAAAA AGATGCATCA ATGAAGCTTT GGTGTGCTGT TGGAGTCAAT
    TTGTCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT GTTTTACAAG
    GATGTTGCTG GTTTGGAAGC AGAAGGCAGT AAACAGCGAA GTGCATCTCA GAATAGCTTA
    GACAAGTTAG ACCAGGAACT TAAGGAACAA CAGAAGGAGT TAAAAAATCA AGAAGAATTA
    TCCAGTCTAG TCTGGATCTG TACCAGCACT CATTCAGCTA CAAAAGTTAT CATTATCGAT
    GCTGTTCAAC CAGGCAACAT CCTAGACAGC TTCACTGTTT GTAACTCTCA CGTTCTGTGT
    ATATCAAGCG TACCAGGAGC CCGAGAAACA GACTACCCTG CAGGAGAAGA GCTTTCAGAA
    TCTGGACAAG TAGACAAAGC ATCTTTATGT GGAAGCATGA CAAGCAATAG CTCAGCAGAG
    ACAGACAGCC TGTTGGGAGG CATCACAGTA GTTGGTTGTT CTGCAGAAGG TGTGACAGCA
    GCTGCCACTT CCCCTAATAC CAATGAACCT TCTCCAGTGA TGGAAAAACC GCCAGCAGAA
    GAAGCAACTG AAGCTACAGA AGGCAATGCA GGATCAGCTG AAGATACTGT GGATGTCTCC
    CAAACTGGCG TGTACACAGA GCATGTGTTT ACAGATCCTT TGGGAGTTCA AATCCCAGAA
    GACCTGTCAC CAGTATATCA GTCAAGTAAT GACTCAGATG CATATAAAGA TCAAATATCA
    GTATTGCCAA ATGAACAAGA CCTGGTGAGA GAGGAAGCCC AGAAAATGAG TAGCCTTTTA
    CCAACTATGT GGCTTGGAGC TCAAAATGGG TGTTTGTATG TCCATTCATC TGTAGCCCAG
    TGGAGGAAAT GTCTCCATTC TATTAAACTT AAAGATTCGA TTCTCAGTAT TGTACACGTG
    AAAGGAATTG TGTTAGTGGC CCTAGCTGAT GGGACCCTTG CCATCTTTCA CAGAGGAGTT
    GATGGACAGT GGGATTTGTC AAACTATCAC CTGTTAGATC TTGGACGACC GCACCATTCC
    ATCCGATGCA TGACTGTGGT ACATGACAAA GTTTGGTGTG GCTATAGGAA CAAAATTTAT
    GTGGTTCAGC CAAAGGCCAT GAAAATAGAG AAATCATTTG ATGCACACCC CAGGAAGGAG
    AGCCAAGTGC GGCAGCTTGC CTGGGTGGGT GATGGCGTGT GGGTCTCCAT TCGCTTGGAT
    TCCACACTCC GTCTCTATCA TGCACACACT TATCAGCATC TGCAGGATGT GGACATTGAG
    CCTTATGTAA GCAAAATGCT AGGTACTGGA AAACTGGGCT TCTCTTTTGT GAGGATCACA
    GCGCTTATGG TGTCTTGTAA CCGTTTGTGG GTGGGAACAG GAAATGGTGT CATTATCTCC
    ATCCCATTGA CAGAAACGAA TAAAACATCA GGAGCATCGG GAAATCGTCC TGGAAGTGTC
    ATTCGAGTAT ATGGTGATGA AAACAGTGAT AAAGTGACAC CAGGGACATT TATACCCTAT
    TGTTCAATGG CACATGCACA GCTTTGCTTC CATGGGCACA GGGATGCTGT GAAATTTTTT
    GTGGCTGTCC CAGGTCAAGT CATCAGCCCA CAAAGTGGCA GCAGTGGTAC AGATCTGACA
    GTTGACAAAG CAGGGCCATC TGCACAGGAG CCTGGCATCC AGACACCCTT GAAGTCTGTG
    CTTGTCATTA GTGGAGGAGA GGGCTACATT GACTTCCGAA TGGGTGATGA AGGTGGAGAA
    TCAGAGCTTC TTGGAGAGGA TCTTCCACTT GAACCTTCTG TCGCCAAAGC AGAAAGGAGT
    CACTTGATAG TGTGGCAAGT GATGTATGGT AGTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836138.1
    CDS176..4054
    Translation

    Target ORF information:

    RefSeq Version XM_024980370.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACTACTA AGAAGCCCGA ACCACCAGTT
    AATCTGAAGT ACAATGCACC AACCTCTCAC GTTACGCCTT CCGTCAAGAA AAGAAGCAGC
    ACCTTATCTC AACTCCCTGG GGATAAGTCC AGAGCCTTTG ATTTCCTTAG CGAAGAAACT
    GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAAC AGTATCGTCA GGTAAAGGCC
    CATGTTCAGA AGGAAGATGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAATAC
    AAACAGGTAA CCAACGGACA AGGGGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT
    CTCAGACCTC TAGATGAAAA AGATGCATCA ATGAAGCTTT GGTGTGCTGT TGGAGTCAAT
    TTGTCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT GTTTTACAAG
    GATGTTGCTG GTTTGGAAGC AGAAGGCAGT AAACAGCGAA GTGCATCTCA GAATAGCTTA
    GACAAGTTAG ACCAGGAACT TAAGGAACAA CAGAAGGAGT TAAAAAATCA AGAAGAATTA
    TCCAGTCTAG TCTGGATCTG TACCAGCACT CATTCAGCTA CAAAAGTTAT CATTATCGAT
    GCTGTTCAAC CAGGCAACAT CCTAGACAGC TTCACTGTTT GTAACTCTCA CGTTCTGTGT
    ATATCAAGCG TACCAGGAGC CCGAGAAACA GACTACCCTG CAGGAGAAGA GCTTTCAGAA
    TCTGGACAAG TAGACAAAGC ATCTTTATGT GGAAGCATGA CAAGCAATAG CTCAGCAGAG
    ACAGACAGCC TGTTGGGAGG CATCACAGTA GTTGGTTGTT CTGCAGAAGG TGTGACAGCA
    GCTGCCACTT CCCCTAATAC CAATGAACCT TCTCCAGTGA TGGAAAAACC GCCAGCAGAA
    GAAGCAACTG AAGCTACAGA AGGCAATGCA GGATCAGCTG AAGATACTGT GGATGTCTCC
    CAAACTGGCG TGTACACAGA GCATGTGTTT ACAGATCCTT TGGGAGTTCA AATCCCAGAA
    GACCTGTCAC CAGTATATCA GTCAAGTAAT GACTCAGATG CATATAAAGA TCAAATATCA
    GTATTGCCAA ATGAACAAGA CCTGGTGAGA GAGGAAGCCC AGAAAATGAG TAGCCTTTTA
    CCAACTATGT GGCTTGGAGC TCAAAATGGG TGTTTGTATG TCCATTCATC TGTAGCCCAG
    TGGAGGAAAT GTCTCCATTC TATTAAACTT AAAGATTCGA TTCTCAGTAT TGTACACGTG
    AAAGGAATTG TGTTAGTGGC CCTAGCTGAT GGGACCCTTG CCATCTTTCA CAGAGGAGTT
    GATGGACAGT GGGATTTGTC AAACTATCAC CTGTTAGATC TTGGACGACC GCACCATTCC
    ATCCGATGCA TGACTGTGGT ACATGACAAA GTTTGGTGTG GCTATAGGAA CAAAATTTAT
    GTGGTTCAGC CAAAGGCCAT GAAAATAGAG AAATCATTTG ATGCACACCC CAGGAAGGAG
    AGCCAAGTGC GGCAGCTTGC CTGGGTGGGT GATGGCGTGT GGGTCTCCAT TCGCTTGGAT
    TCCACACTCC GTCTCTATCA TGCACACACT TATCAGCATC TGCAGGATGT GGACATTGAG
    CCTTATGTAA GCAAAATGCT AGGTACTGGA AAACTGGGCT TCTCTTTTGT GAGGATCACA
    GCGCTTATGG TGTCTTGTAA CCGTTTGTGG GTGGGAACAG GAAATGGTGT CATTATCTCC
    ATCCCATTGA CAGAAACGAA TAAAACATCA GGAGCATCGG GAAATCGTCC TGGAAGTGTC
    ATTCGAGTAT ATGGTGATGA AAACAGTGAT AAAGTGACAC CAGGGACATT TATACCCTAT
    TGTTCAATGG CACATGCACA GCTTTGCTTC CATGGGCACA GGGATGCTGT GAAATTTTTT
    GTGGCTGTCC CAGGTCAAGT CATCAGCCCA CAAAGTGGCA GCAGTGGTAC AGATCTGACA
    GTTGACAAAG CAGGGCCATC TGCACAGGAG CCTGGCATCC AGACACCCTT GAAGTCTGTG
    CTTGTCATTA GTGGAGGAGA GGGCTACATT GACTTCCGAA TGGGTGATGA AGGTGGAGAA
    TCAGAGCTTC TTGGAGAGGA TCTTCCACTT GAACCTTCTG TCGCCAAAGC AGAAAGGAGT
    CACTTGATAG TGTGGCAAGT GATGTATGGT AGTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226509
    Clone ID Related Accession (Same CDS sequence) XM_005220620.4
    Accession Version XM_005220620.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220620.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
    AATTCTGCTA TCTTAGTATC TCTCTCCGTC TTGCTGCTTG TGCACTTCTC TATTTCTACC
    GGAGTGCCAG CTTTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG TCCTATATCG
    TTAGGGATTT TCCCTTTACC TTCTGGAGAC GGATTGCTTA CGCCTGACAC TCAGAAAGGT
    GGCGAGACCC CTGGATCAGA ACAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT
    ACCAGTCTGA AGGATGAACT TTCCGATGTT AGCCAGGGAG GATCTAAAGC CACCACTCCA
    GCGTCGACAG CTGCTTCAGA TGTGGCAGCA ACACCTAGCG ATACTCCCTT ACATGAGGAG
    AACGGAGGGG TTGTGGAGGT TGCAGATACA CCCGATAAGT CAGAGATAAG CAAGCATATC
    GAAGTACACG TAGTCCAGGA AACTAGAAAC ATAGCAACGG GTTCTGCTGA AAATGAAGAG
    AAGTCAGAAG TTCAGGCAAT CATTGAATCC ACTCCTGAGT TGGATATGGA CAAAGATCTC
    AGTGGATATA AAGGCTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC
    AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGTT CAGGTCTAAT TGGAGATGTG
    GATGAAGGAG CAGATTTACT AGGAATGGGT CGTGAAGTTG AAAATCTCAT ATTGGAAAAT
    ACACAACTAT TGGAAACCAA AAATGCCTTG AATGTAGTGA AGAATGACTT GATAGCAAAA
    GTAGATGAAC TTACCTGTGA GAAAGATGTG CTGCAAGGGG AGCTGGAGGC TGTGAAACAA
    GCCAAGCTGA AGCTGGAGGA AAAGAACAAA GAATTGGAAG AAGAGCTTAG GAAAGCTCGT
    GCAGAAGTTG AAGATGCAAA GCAAAAAGCA AAAGATGATG ATGATAGTGA TATTCCCACA
    GCCCAAAGGA AGCGGTTTAC TAGAGTAGAA ATGGCCCGAG TTCTAATGGA GAGAAACCAA
    TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGTGCG
    TCAAGAGAAA ATCCAGCCAT GCAGGAGAAG AAAAGGTCAA GCATTTGGCA GTTTTTCAGC
    CGACTTTTCA GCTCCTCAAG TAACACTACT AAGAAGCCCG AACCACCAGT TAATCTGAAG
    TACAATGCAC CAACCTCTCA CGTTACGCCT TCCGTCAAGA AAAGAAGCAG CACCTTATCT
    CAACTCCCTG GGGATAAGTC CAGAGCCTTT GATTTCCTTA GCGAAGAAAC TGAAGCTAGT
    TTAGCCTCAC GCAGAGAACA AAAGAGAGAA CAGTATCGTC AGGTAAAGGC CCATGTTCAG
    AAGGAAGATG GTAGAGTGCA GGCTTTTGGC TGGAGTCTGC CTCAGAAATA CAAACAGGTA
    ACCAACGGAC AAGGGGAAAA TAAGATGAAA AATTTACCTG TGCCTGTCTA TCTCAGACCT
    CTAGATGAAA AAGATGCATC AATGAAGCTT TGGTGTGCTG TTGGAGTCAA TTTGTCTGGT
    GGGAAGACCA GAGATGGTGG TTCTGTTGTT GGAGCAAGTG TGTTTTACAA GGATGTTGCT
    GGTTTGGAAG CAGAAGGCAG TAAACAGCGA AGTGCATCTC AGAATAGCTT AGACAAGTTA
    GACCAGGAAC TTAAGGAACA ACAGAAGGAG TTAAAAAATC AAGAAGAATT ATCCAGTCTA
    GTCTGGATCT GTACCAGCAC TCATTCAGCT ACAAAAGTTA TCATTATCGA TGCTGTTCAA
    CCAGGCAACA TCCTAGACAG CTTCACTGTT TGTAACTCTC ACGTTCTGTG TATATCAAGC
    GTACCAGGAG CCCGAGAAAC AGACTACCCT GCAGGAGAAG AGCTTTCAGA ATCTGGACAA
    GTAGACAAAG CATCTTTATG TGGAAGCATG ACAAGCAATA GCTCAGCAGA GACAGACAGC
    CTGTTGGGAG GCATCACAGT AGTTGGTTGT TCTGCAGAAG GTGTGACAGC AGCTGCCACT
    TCCCCTAATA CCAATGAACC TTCTCCAGTG ATGGAAAAAC CGCCAGAAAA GGAAACAGAA
    AATAGTGAGA TTGATGAAAA TGTTCCAACA GCAGAAGAAG CAACTGAAGC TACAGAAGGC
    AATGCAGGAT CAGCTGAAGA TACTGTGGAT GTCTCCCAAA CTGGCGTGTA CACAGAGCAT
    GTGTTTACAG ATCCTTTGGG AGTTCAAATC CCAGAAGACC TGTCACCAGT ATATCAGTCA
    AGTAATGACT CAGATGCATA TAAAGATCAA ATATCAGTAT TGCCAAATGA ACAAGACCTG
    GTGAGAGAGG AAGCCCAGAA AATGAGTAGC CTTTTACCAA CTATGTGGCT TGGAGCTCAA
    AATGGGTGTT TGTATGTCCA TTCATCTGTA GCCCAGTGGA GGAAATGTCT CCATTCTATT
    AAACTTAAAG ATTCGATTCT CAGTATTGTA CACGTGAAAG GAATTGTGTT AGTGGCCCTA
    GCTGATGGGA CCCTTGCCAT CTTTCACAGA GGAGTTGATG GACAGTGGGA TTTGTCAAAC
    TATCACCTGT TAGATCTTGG ACGACCGCAC CATTCCATCC GATGCATGAC TGTGGTACAT
    GACAAAGTTT GGTGTGGCTA TAGGAACAAA ATTTATGTGG TTCAGCCAAA GGCCATGAAA
    ATAGAGAAAT CATTTGATGC ACACCCCAGG AAGGAGAGCC AAGTGCGGCA GCTTGCCTGG
    GTGGGTGATG GCGTGTGGGT CTCCATTCGC TTGGATTCCA CACTCCGTCT CTATCATGCA
    CACACTTATC AGCATCTGCA GGATGTGGAC ATTGAGCCTT ATGTAAGCAA AATGCTAGGT
    ACTGGAAAAC TGGGCTTCTC TTTTGTGAGG ATCACAGCGC TTATGGTGTC TTGTAACCGT
    TTGTGGGTGG GAACAGGAAA TGGTGTCATT ATCTCCATCC CATTGACAGA AACGAATAAA
    ACATCAGGAG CATCGGGAAA TCGTCCTGGA AGTGTCATTC GAGTATATGG TGATGAAAAC
    AGTGATAAAG TGACACCAGG GACATTTATA CCCTATTGTT CAATGGCACA TGCACAGCTT
    TGCTTCCATG GGCACAGGGA TGCTGTGAAA TTTTTTGTGG CTGTCCCAGG TCAAGTCATC
    AGCCCACAAA GTGGCAGCAG TGGTACAGAT CTGACAGTTG ACAAAGCAGG GCCATCTGCA
    CAGGAGCCTG GCATCCAGAC ACCCTTGAAG TCTGTGCTTG TCATTAGTGG AGGAGAGGGC
    TACATTGACT TCCGAATGGG TGATGAAGGT GGAGAATCAG AGCTTCTTGG AGAGGATCTT
    CCACTTGAAC CTTCTGTCGC CAAAGCAGAA AGGAGTCACT TGATAGTGTG GCAAGTGATG
    TATGGTAGTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220677.1
    CDS134..3628
    Translation

    Target ORF information:

    RefSeq Version XM_005220620.4
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220620.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
    AATTCTGCTA TCTTAGTATC TCTCTCCGTC TTGCTGCTTG TGCACTTCTC TATTTCTACC
    GGAGTGCCAG CTTTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG TCCTATATCG
    TTAGGGATTT TCCCTTTACC TTCTGGAGAC GGATTGCTTA CGCCTGACAC TCAGAAAGGT
    GGCGAGACCC CTGGATCAGA ACAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT
    ACCAGTCTGA AGGATGAACT TTCCGATGTT AGCCAGGGAG GATCTAAAGC CACCACTCCA
    GCGTCGACAG CTGCTTCAGA TGTGGCAGCA ACACCTAGCG ATACTCCCTT ACATGAGGAG
    AACGGAGGGG TTGTGGAGGT TGCAGATACA CCCGATAAGT CAGAGATAAG CAAGCATATC
    GAAGTACACG TAGTCCAGGA AACTAGAAAC ATAGCAACGG GTTCTGCTGA AAATGAAGAG
    AAGTCAGAAG TTCAGGCAAT CATTGAATCC ACTCCTGAGT TGGATATGGA CAAAGATCTC
    AGTGGATATA AAGGCTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC
    AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGTT CAGGTCTAAT TGGAGATGTG
    GATGAAGGAG CAGATTTACT AGGAATGGGT CGTGAAGTTG AAAATCTCAT ATTGGAAAAT
    ACACAACTAT TGGAAACCAA AAATGCCTTG AATGTAGTGA AGAATGACTT GATAGCAAAA
    GTAGATGAAC TTACCTGTGA GAAAGATGTG CTGCAAGGGG AGCTGGAGGC TGTGAAACAA
    GCCAAGCTGA AGCTGGAGGA AAAGAACAAA GAATTGGAAG AAGAGCTTAG GAAAGCTCGT
    GCAGAAGTTG AAGATGCAAA GCAAAAAGCA AAAGATGATG ATGATAGTGA TATTCCCACA
    GCCCAAAGGA AGCGGTTTAC TAGAGTAGAA ATGGCCCGAG TTCTAATGGA GAGAAACCAA
    TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGTGCG
    TCAAGAGAAA ATCCAGCCAT GCAGGAGAAG AAAAGGTCAA GCATTTGGCA GTTTTTCAGC
    CGACTTTTCA GCTCCTCAAG TAACACTACT AAGAAGCCCG AACCACCAGT TAATCTGAAG
    TACAATGCAC CAACCTCTCA CGTTACGCCT TCCGTCAAGA AAAGAAGCAG CACCTTATCT
    CAACTCCCTG GGGATAAGTC CAGAGCCTTT GATTTCCTTA GCGAAGAAAC TGAAGCTAGT
    TTAGCCTCAC GCAGAGAACA AAAGAGAGAA CAGTATCGTC AGGTAAAGGC CCATGTTCAG
    AAGGAAGATG GTAGAGTGCA GGCTTTTGGC TGGAGTCTGC CTCAGAAATA CAAACAGGTA
    ACCAACGGAC AAGGGGAAAA TAAGATGAAA AATTTACCTG TGCCTGTCTA TCTCAGACCT
    CTAGATGAAA AAGATGCATC AATGAAGCTT TGGTGTGCTG TTGGAGTCAA TTTGTCTGGT
    GGGAAGACCA GAGATGGTGG TTCTGTTGTT GGAGCAAGTG TGTTTTACAA GGATGTTGCT
    GGTTTGGAAG CAGAAGGCAG TAAACAGCGA AGTGCATCTC AGAATAGCTT AGACAAGTTA
    GACCAGGAAC TTAAGGAACA ACAGAAGGAG TTAAAAAATC AAGAAGAATT ATCCAGTCTA
    GTCTGGATCT GTACCAGCAC TCATTCAGCT ACAAAAGTTA TCATTATCGA TGCTGTTCAA
    CCAGGCAACA TCCTAGACAG CTTCACTGTT TGTAACTCTC ACGTTCTGTG TATATCAAGC
    GTACCAGGAG CCCGAGAAAC AGACTACCCT GCAGGAGAAG AGCTTTCAGA ATCTGGACAA
    GTAGACAAAG CATCTTTATG TGGAAGCATG ACAAGCAATA GCTCAGCAGA GACAGACAGC
    CTGTTGGGAG GCATCACAGT AGTTGGTTGT TCTGCAGAAG GTGTGACAGC AGCTGCCACT
    TCCCCTAATA CCAATGAACC TTCTCCAGTG ATGGAAAAAC CGCCAGAAAA GGAAACAGAA
    AATAGTGAGA TTGATGAAAA TGTTCCAACA GCAGAAGAAG CAACTGAAGC TACAGAAGGC
    AATGCAGGAT CAGCTGAAGA TACTGTGGAT GTCTCCCAAA CTGGCGTGTA CACAGAGCAT
    GTGTTTACAG ATCCTTTGGG AGTTCAAATC CCAGAAGACC TGTCACCAGT ATATCAGTCA
    AGTAATGACT CAGATGCATA TAAAGATCAA ATATCAGTAT TGCCAAATGA ACAAGACCTG
    GTGAGAGAGG AAGCCCAGAA AATGAGTAGC CTTTTACCAA CTATGTGGCT TGGAGCTCAA
    AATGGGTGTT TGTATGTCCA TTCATCTGTA GCCCAGTGGA GGAAATGTCT CCATTCTATT
    AAACTTAAAG ATTCGATTCT CAGTATTGTA CACGTGAAAG GAATTGTGTT AGTGGCCCTA
    GCTGATGGGA CCCTTGCCAT CTTTCACAGA GGAGTTGATG GACAGTGGGA TTTGTCAAAC
    TATCACCTGT TAGATCTTGG ACGACCGCAC CATTCCATCC GATGCATGAC TGTGGTACAT
    GACAAAGTTT GGTGTGGCTA TAGGAACAAA ATTTATGTGG TTCAGCCAAA GGCCATGAAA
    ATAGAGAAAT CATTTGATGC ACACCCCAGG AAGGAGAGCC AAGTGCGGCA GCTTGCCTGG
    GTGGGTGATG GCGTGTGGGT CTCCATTCGC TTGGATTCCA CACTCCGTCT CTATCATGCA
    CACACTTATC AGCATCTGCA GGATGTGGAC ATTGAGCCTT ATGTAAGCAA AATGCTAGGT
    ACTGGAAAAC TGGGCTTCTC TTTTGTGAGG ATCACAGCGC TTATGGTGTC TTGTAACCGT
    TTGTGGGTGG GAACAGGAAA TGGTGTCATT ATCTCCATCC CATTGACAGA AACGAATAAA
    ACATCAGGAG CATCGGGAAA TCGTCCTGGA AGTGTCATTC GAGTATATGG TGATGAAAAC
    AGTGATAAAG TGACACCAGG GACATTTATA CCCTATTGTT CAATGGCACA TGCACAGCTT
    TGCTTCCATG GGCACAGGGA TGCTGTGAAA TTTTTTGTGG CTGTCCCAGG TCAAGTCATC
    AGCCCACAAA GTGGCAGCAG TGGTACAGAT CTGACAGTTG ACAAAGCAGG GCCATCTGCA
    CAGGAGCCTG GCATCCAGAC ACCCTTGAAG TCTGTGCTTG TCATTAGTGG AGGAGAGGGC
    TACATTGACT TCCGAATGGG TGATGAAGGT GGAGAATCAG AGCTTCTTGG AGAGGATCTT
    CCACTTGAAC CTTCTGTCGC CAAAGCAGAA AGGAGTCACT TGATAGTGTG GCAAGTGATG
    TATGGTAGTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226510
    Clone ID Related Accession (Same CDS sequence) XM_024980371.1 , XM_024980372.1
    Accession Version XM_024980371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTTTGCA TCAGAGACAC ACTGAGAAAA GAACGTCCTA TATCGTTAGG GATTTTCCCT 
    TTACCTTCTG GAGACGGATT GCTTACGCCT GACACTCAGA AAGGTGGCGA GACCCCTGGA
    TCAGAACAAT GGAAATTTCA GGAATTAAGT CAACCACGTT CTCATACCAG TCTGAAGGAT
    GAACTTTCCG ATGTTAGCCA GGGAGGATCT AAAGCCACCA CTCCAGCGTC GACAGCTGCT
    TCAGATGTGG CAGCAACACC TAGCGATACT CCCTTACATG AGGAGAACGG AGGGGTTGTG
    GAGGTTGCAG ATACACCCGA TAAGTCAGAG ATAAGCAAGC ATATCGAAGT ACACGTAGTC
    CAGGAAACTA GAAACATAGC AACGGGTTCT GCTGAAAATG AAGAGAAGTC AGAAGTTCAG
    GCAATCATTG AATCCACTCC TGAGTTGGAT ATGGACAAAG ATCTCAGTGG ATATAAAGGC
    TCAAGCACTC CCACCAAAGG CATAGAGAAC AAAGCTTTTG ATCGCAATAC AGAATCTCTC
    TTTGAAGAAC TGTCTTCAGC TGGTTCAGGT CTAATTGGAG ATGTGGATGA AGGAGCAGAT
    TTACTAGGAA TGGGTCGTGA AGTTGAAAAT CTCATATTGG AAAATACACA ACTATTGGAA
    ACCAAAAATG CCTTGAATGT AGTGAAGAAT GACTTGATAG CAAAAGTAGA TGAACTTACC
    TGTGAGAAAG ATGTGCTGCA AGGGGAGCTG GAGGCTGTGA AACAAGCCAA GCTGAAGCTG
    GAGGAAAAGA ACAAAGAATT GGAAGAAGAG CTTAGGAAAG CTCGTGCAGA AGTTGAAGAT
    GCAAAGCAAA AAGCAAAAGA TGATGATGAT AGTGATATTC CCACAGCCCA AAGGAAGCGG
    TTTACTAGAG TAGAAATGGC CCGAGTTCTA ATGGAGAGAA ACCAATATAA AGAGAGATTG
    ATGGAGCTTC AGGAAGCTGT TCGATGGACA GAGATGATTC GTGCGTCAAG AGAAAATCCA
    GCCATGCAGG AGAAGAAAAG GTCAAGCATT TGGCAGTTTT TCAGCCGACT TTTCAGCTCC
    TCAAGTAACA CTACTAAGAA GCCCGAACCA CCAGTTAATC TGAAGTACAA TGCACCAACC
    TCTCACGTTA CGCCTTCCGT CAAGAAAAGA AGCAGCACCT TATCTCAACT CCCTGGGGAT
    AAGTCCAGAG CCTTTGATTT CCTTAGCGAA GAAACTGAAG CTAGTTTAGC CTCACGCAGA
    GAACAAAAGA GAGAACAGTA TCGTCAGGTA AAGGCCCATG TTCAGAAGGA AGATGGTAGA
    GTGCAGGCTT TTGGCTGGAG TCTGCCTCAG AAATACAAAC AGGTAACCAA CGGACAAGGG
    GAAAATAAGA TGAAAAATTT ACCTGTGCCT GTCTATCTCA GACCTCTAGA TGAAAAAGAT
    GCATCAATGA AGCTTTGGTG TGCTGTTGGA GTCAATTTGT CTGGTGGGAA GACCAGAGAT
    GGTGGTTCTG TTGTTGGAGC AAGTGTGTTT TACAAGGATG TTGCTGGTTT GGAAGCAGAA
    GGCAGTAAAC AGCGAAGTGC ATCTCAGAAT AGCTTAGACA AGTTAGACCA GGAACTTAAG
    GAACAACAGA AGGAGTTAAA AAATCAAGAA GAATTATCCA GTCTAGTCTG GATCTGTACC
    AGCACTCATT CAGCTACAAA AGTTATCATT ATCGATGCTG TTCAACCAGG CAACATCCTA
    GACAGCTTCA CTGTTTGTAA CTCTCACGTT CTGTGTATAT CAAGCGTACC AGGAGCCCGA
    GAAACAGACT ACCCTGCAGG AGAAGAGCTT TCAGAATCTG GACAAGTAGA CAAAGCATCT
    TTATGTGGAA GCATGACAAG CAATAGCTCA GCAGAGACAG ACAGCCTGTT GGGAGGCATC
    ACAGTAGTTG GTTGTTCTGC AGAAGGTGTG ACAGCAGCTG CCACTTCCCC TAATACCAAT
    GAACCTTCTC CAGTGATGGA AAAACCGCCA GAAAAGGAAA CAGAAAATAG TGAGATTGAT
    GAAAATGTTC CAACAGCAGA AGAAGCAACT GAAGCTACAG AAGGCAATGC AGGATCAGCT
    GAAGATACTG TGGATGTCTC CCAAACTGGC GTGTACACAG AGCATGTGTT TACAGATCCT
    TTGGGAGTTC AAATCCCAGA AGACCTGTCA CCAGTATATC AGTCAAGTAA TGACTCAGAT
    GCATATAAAG ATCAAATATC AGTATTGCCA AATGAACAAG ACCTGGTGAG AGAGGAAGCC
    CAGAAAATGA GTAGCCTTTT ACCAACTATG TGGCTTGGAG CTCAAAATGG GTGTTTGTAT
    GTCCATTCAT CTGTAGCCCA GTGGAGGAAA TGTCTCCATT CTATTAAACT TAAAGATTCG
    ATTCTCAGTA TTGTACACGT GAAAGGAATT GTGTTAGTGG CCCTAGCTGA TGGGACCCTT
    GCCATCTTTC ACAGAGGAGT TGATGGACAG TGGGATTTGT CAAACTATCA CCTGTTAGAT
    CTTGGACGAC CGCACCATTC CATCCGATGC ATGACTGTGG TACATGACAA AGTTTGGTGT
    GGCTATAGGA ACAAAATTTA TGTGGTTCAG CCAAAGGCCA TGAAAATAGA GAAATCATTT
    GATGCACACC CCAGGAAGGA GAGCCAAGTG CGGCAGCTTG CCTGGGTGGG TGATGGCGTG
    TGGGTCTCCA TTCGCTTGGA TTCCACACTC CGTCTCTATC ATGCACACAC TTATCAGCAT
    CTGCAGGATG TGGACATTGA GCCTTATGTA AGCAAAATGC TAGGTACTGG AAAACTGGGC
    TTCTCTTTTG TGAGGATCAC AGCGCTTATG GTGTCTTGTA ACCGTTTGTG GGTGGGAACA
    GGAAATGGTG TCATTATCTC CATCCCATTG ACAGAAACCG TAATCCTCCA CCAGGGACGT
    TTACTGGGGC TGAGGGCGAA TAAAACATCA GGAGCATCGG GAAATCGTCC TGGAAGTGTC
    ATTCGAGTAT ATGGTGATGA AAACAGTGAT AAAGTGACAC CAGGGACATT TATACCCTAT
    TGTTCAATGG CACATGCACA GCTTTGCTTC CATGGGCACA GGGATGCTGT GAAATTTTTT
    GTGGCTGTCC CAGGTCAAGT CATCAGCCCA CAAAGTGGCA GCAGTGGTAC AGATCTGACA
    GTTGACAAAG CAGGGCCATC TGCACAGGAG CCTGGCATCC AGACACCCTT GAAGTCTGTG
    CTTGTCATTA GTGGAGGAGA GGGCTACATT GACTTCCGAA TGGGTGATGA AGGTGGAGAA
    TCAGAGCTTC TTGGAGAGGA TCTTCCACTT GAACCTTCTG TCGCCAAAGC AGAAAGGAGT
    CACTTGATAG TGTGGCAAGT GATGTATGGT AGTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836139.1
    CDS167..3565
    Translation

    Target ORF information:

    RefSeq Version XM_024980371.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTTTGCA TCAGAGACAC ACTGAGAAAA GAACGTCCTA TATCGTTAGG GATTTTCCCT 
    TTACCTTCTG GAGACGGATT GCTTACGCCT GACACTCAGA AAGGTGGCGA GACCCCTGGA
    TCAGAACAAT GGAAATTTCA GGAATTAAGT CAACCACGTT CTCATACCAG TCTGAAGGAT
    GAACTTTCCG ATGTTAGCCA GGGAGGATCT AAAGCCACCA CTCCAGCGTC GACAGCTGCT
    TCAGATGTGG CAGCAACACC TAGCGATACT CCCTTACATG AGGAGAACGG AGGGGTTGTG
    GAGGTTGCAG ATACACCCGA TAAGTCAGAG ATAAGCAAGC ATATCGAAGT ACACGTAGTC
    CAGGAAACTA GAAACATAGC AACGGGTTCT GCTGAAAATG AAGAGAAGTC AGAAGTTCAG
    GCAATCATTG AATCCACTCC TGAGTTGGAT ATGGACAAAG ATCTCAGTGG ATATAAAGGC
    TCAAGCACTC CCACCAAAGG CATAGAGAAC AAAGCTTTTG ATCGCAATAC AGAATCTCTC
    TTTGAAGAAC TGTCTTCAGC TGGTTCAGGT CTAATTGGAG ATGTGGATGA AGGAGCAGAT
    TTACTAGGAA TGGGTCGTGA AGTTGAAAAT CTCATATTGG AAAATACACA ACTATTGGAA
    ACCAAAAATG CCTTGAATGT AGTGAAGAAT GACTTGATAG CAAAAGTAGA TGAACTTACC
    TGTGAGAAAG ATGTGCTGCA AGGGGAGCTG GAGGCTGTGA AACAAGCCAA GCTGAAGCTG
    GAGGAAAAGA ACAAAGAATT GGAAGAAGAG CTTAGGAAAG CTCGTGCAGA AGTTGAAGAT
    GCAAAGCAAA AAGCAAAAGA TGATGATGAT AGTGATATTC CCACAGCCCA AAGGAAGCGG
    TTTACTAGAG TAGAAATGGC CCGAGTTCTA ATGGAGAGAA ACCAATATAA AGAGAGATTG
    ATGGAGCTTC AGGAAGCTGT TCGATGGACA GAGATGATTC GTGCGTCAAG AGAAAATCCA
    GCCATGCAGG AGAAGAAAAG GTCAAGCATT TGGCAGTTTT TCAGCCGACT TTTCAGCTCC
    TCAAGTAACA CTACTAAGAA GCCCGAACCA CCAGTTAATC TGAAGTACAA TGCACCAACC
    TCTCACGTTA CGCCTTCCGT CAAGAAAAGA AGCAGCACCT TATCTCAACT CCCTGGGGAT
    AAGTCCAGAG CCTTTGATTT CCTTAGCGAA GAAACTGAAG CTAGTTTAGC CTCACGCAGA
    GAACAAAAGA GAGAACAGTA TCGTCAGGTA AAGGCCCATG TTCAGAAGGA AGATGGTAGA
    GTGCAGGCTT TTGGCTGGAG TCTGCCTCAG AAATACAAAC AGGTAACCAA CGGACAAGGG
    GAAAATAAGA TGAAAAATTT ACCTGTGCCT GTCTATCTCA GACCTCTAGA TGAAAAAGAT
    GCATCAATGA AGCTTTGGTG TGCTGTTGGA GTCAATTTGT CTGGTGGGAA GACCAGAGAT
    GGTGGTTCTG TTGTTGGAGC AAGTGTGTTT TACAAGGATG TTGCTGGTTT GGAAGCAGAA
    GGCAGTAAAC AGCGAAGTGC ATCTCAGAAT AGCTTAGACA AGTTAGACCA GGAACTTAAG
    GAACAACAGA AGGAGTTAAA AAATCAAGAA GAATTATCCA GTCTAGTCTG GATCTGTACC
    AGCACTCATT CAGCTACAAA AGTTATCATT ATCGATGCTG TTCAACCAGG CAACATCCTA
    GACAGCTTCA CTGTTTGTAA CTCTCACGTT CTGTGTATAT CAAGCGTACC AGGAGCCCGA
    GAAACAGACT ACCCTGCAGG AGAAGAGCTT TCAGAATCTG GACAAGTAGA CAAAGCATCT
    TTATGTGGAA GCATGACAAG CAATAGCTCA GCAGAGACAG ACAGCCTGTT GGGAGGCATC
    ACAGTAGTTG GTTGTTCTGC AGAAGGTGTG ACAGCAGCTG CCACTTCCCC TAATACCAAT
    GAACCTTCTC CAGTGATGGA AAAACCGCCA GAAAAGGAAA CAGAAAATAG TGAGATTGAT
    GAAAATGTTC CAACAGCAGA AGAAGCAACT GAAGCTACAG AAGGCAATGC AGGATCAGCT
    GAAGATACTG TGGATGTCTC CCAAACTGGC GTGTACACAG AGCATGTGTT TACAGATCCT
    TTGGGAGTTC AAATCCCAGA AGACCTGTCA CCAGTATATC AGTCAAGTAA TGACTCAGAT
    GCATATAAAG ATCAAATATC AGTATTGCCA AATGAACAAG ACCTGGTGAG AGAGGAAGCC
    CAGAAAATGA GTAGCCTTTT ACCAACTATG TGGCTTGGAG CTCAAAATGG GTGTTTGTAT
    GTCCATTCAT CTGTAGCCCA GTGGAGGAAA TGTCTCCATT CTATTAAACT TAAAGATTCG
    ATTCTCAGTA TTGTACACGT GAAAGGAATT GTGTTAGTGG CCCTAGCTGA TGGGACCCTT
    GCCATCTTTC ACAGAGGAGT TGATGGACAG TGGGATTTGT CAAACTATCA CCTGTTAGAT
    CTTGGACGAC CGCACCATTC CATCCGATGC ATGACTGTGG TACATGACAA AGTTTGGTGT
    GGCTATAGGA ACAAAATTTA TGTGGTTCAG CCAAAGGCCA TGAAAATAGA GAAATCATTT
    GATGCACACC CCAGGAAGGA GAGCCAAGTG CGGCAGCTTG CCTGGGTGGG TGATGGCGTG
    TGGGTCTCCA TTCGCTTGGA TTCCACACTC CGTCTCTATC ATGCACACAC TTATCAGCAT
    CTGCAGGATG TGGACATTGA GCCTTATGTA AGCAAAATGC TAGGTACTGG AAAACTGGGC
    TTCTCTTTTG TGAGGATCAC AGCGCTTATG GTGTCTTGTA ACCGTTTGTG GGTGGGAACA
    GGAAATGGTG TCATTATCTC CATCCCATTG ACAGAAACCG TAATCCTCCA CCAGGGACGT
    TTACTGGGGC TGAGGGCGAA TAAAACATCA GGAGCATCGG GAAATCGTCC TGGAAGTGTC
    ATTCGAGTAT ATGGTGATGA AAACAGTGAT AAAGTGACAC CAGGGACATT TATACCCTAT
    TGTTCAATGG CACATGCACA GCTTTGCTTC CATGGGCACA GGGATGCTGT GAAATTTTTT
    GTGGCTGTCC CAGGTCAAGT CATCAGCCCA CAAAGTGGCA GCAGTGGTAC AGATCTGACA
    GTTGACAAAG CAGGGCCATC TGCACAGGAG CCTGGCATCC AGACACCCTT GAAGTCTGTG
    CTTGTCATTA GTGGAGGAGA GGGCTACATT GACTTCCGAA TGGGTGATGA AGGTGGAGAA
    TCAGAGCTTC TTGGAGAGGA TCTTCCACTT GAACCTTCTG TCGCCAAAGC AGAAAGGAGT
    CACTTGATAG TGTGGCAAGT GATGTATGGT AGTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226510
    Clone ID Related Accession (Same CDS sequence) XM_024980371.1 , XM_024980372.1
    Accession Version XM_024980372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTTTGCA TCAGAGACAC ACTGAGAAAA GAACGTCCTA TATCGTTAGG GATTTTCCCT 
    TTACCTTCTG GAGACGGATT GCTTACGCCT GACACTCAGA AAGGTGGCGA GACCCCTGGA
    TCAGAACAAT GGAAATTTCA GGAATTAAGT CAACCACGTT CTCATACCAG TCTGAAGGAT
    GAACTTTCCG ATGTTAGCCA GGGAGGATCT AAAGCCACCA CTCCAGCGTC GACAGCTGCT
    TCAGATGTGG CAGCAACACC TAGCGATACT CCCTTACATG AGGAGAACGG AGGGGTTGTG
    GAGGTTGCAG ATACACCCGA TAAGTCAGAG ATAAGCAAGC ATATCGAAGT ACACGTAGTC
    CAGGAAACTA GAAACATAGC AACGGGTTCT GCTGAAAATG AAGAGAAGTC AGAAGTTCAG
    GCAATCATTG AATCCACTCC TGAGTTGGAT ATGGACAAAG ATCTCAGTGG ATATAAAGGC
    TCAAGCACTC CCACCAAAGG CATAGAGAAC AAAGCTTTTG ATCGCAATAC AGAATCTCTC
    TTTGAAGAAC TGTCTTCAGC TGGTTCAGGT CTAATTGGAG ATGTGGATGA AGGAGCAGAT
    TTACTAGGAA TGGGTCGTGA AGTTGAAAAT CTCATATTGG AAAATACACA ACTATTGGAA
    ACCAAAAATG CCTTGAATGT AGTGAAGAAT GACTTGATAG CAAAAGTAGA TGAACTTACC
    TGTGAGAAAG ATGTGCTGCA AGGGGAGCTG GAGGCTGTGA AACAAGCCAA GCTGAAGCTG
    GAGGAAAAGA ACAAAGAATT GGAAGAAGAG CTTAGGAAAG CTCGTGCAGA AGTTGAAGAT
    GCAAAGCAAA AAGCAAAAGA TGATGATGAT AGTGATATTC CCACAGCCCA AAGGAAGCGG
    TTTACTAGAG TAGAAATGGC CCGAGTTCTA ATGGAGAGAA ACCAATATAA AGAGAGATTG
    ATGGAGCTTC AGGAAGCTGT TCGATGGACA GAGATGATTC GTGCGTCAAG AGAAAATCCA
    GCCATGCAGG AGAAGAAAAG GTCAAGCATT TGGCAGTTTT TCAGCCGACT TTTCAGCTCC
    TCAAGTAACA CTACTAAGAA GCCCGAACCA CCAGTTAATC TGAAGTACAA TGCACCAACC
    TCTCACGTTA CGCCTTCCGT CAAGAAAAGA AGCAGCACCT TATCTCAACT CCCTGGGGAT
    AAGTCCAGAG CCTTTGATTT CCTTAGCGAA GAAACTGAAG CTAGTTTAGC CTCACGCAGA
    GAACAAAAGA GAGAACAGTA TCGTCAGGTA AAGGCCCATG TTCAGAAGGA AGATGGTAGA
    GTGCAGGCTT TTGGCTGGAG TCTGCCTCAG AAATACAAAC AGGTAACCAA CGGACAAGGG
    GAAAATAAGA TGAAAAATTT ACCTGTGCCT GTCTATCTCA GACCTCTAGA TGAAAAAGAT
    GCATCAATGA AGCTTTGGTG TGCTGTTGGA GTCAATTTGT CTGGTGGGAA GACCAGAGAT
    GGTGGTTCTG TTGTTGGAGC AAGTGTGTTT TACAAGGATG TTGCTGGTTT GGAAGCAGAA
    GGCAGTAAAC AGCGAAGTGC ATCTCAGAAT AGCTTAGACA AGTTAGACCA GGAACTTAAG
    GAACAACAGA AGGAGTTAAA AAATCAAGAA GAATTATCCA GTCTAGTCTG GATCTGTACC
    AGCACTCATT CAGCTACAAA AGTTATCATT ATCGATGCTG TTCAACCAGG CAACATCCTA
    GACAGCTTCA CTGTTTGTAA CTCTCACGTT CTGTGTATAT CAAGCGTACC AGGAGCCCGA
    GAAACAGACT ACCCTGCAGG AGAAGAGCTT TCAGAATCTG GACAAGTAGA CAAAGCATCT
    TTATGTGGAA GCATGACAAG CAATAGCTCA GCAGAGACAG ACAGCCTGTT GGGAGGCATC
    ACAGTAGTTG GTTGTTCTGC AGAAGGTGTG ACAGCAGCTG CCACTTCCCC TAATACCAAT
    GAACCTTCTC CAGTGATGGA AAAACCGCCA GAAAAGGAAA CAGAAAATAG TGAGATTGAT
    GAAAATGTTC CAACAGCAGA AGAAGCAACT GAAGCTACAG AAGGCAATGC AGGATCAGCT
    GAAGATACTG TGGATGTCTC CCAAACTGGC GTGTACACAG AGCATGTGTT TACAGATCCT
    TTGGGAGTTC AAATCCCAGA AGACCTGTCA CCAGTATATC AGTCAAGTAA TGACTCAGAT
    GCATATAAAG ATCAAATATC AGTATTGCCA AATGAACAAG ACCTGGTGAG AGAGGAAGCC
    CAGAAAATGA GTAGCCTTTT ACCAACTATG TGGCTTGGAG CTCAAAATGG GTGTTTGTAT
    GTCCATTCAT CTGTAGCCCA GTGGAGGAAA TGTCTCCATT CTATTAAACT TAAAGATTCG
    ATTCTCAGTA TTGTACACGT GAAAGGAATT GTGTTAGTGG CCCTAGCTGA TGGGACCCTT
    GCCATCTTTC ACAGAGGAGT TGATGGACAG TGGGATTTGT CAAACTATCA CCTGTTAGAT
    CTTGGACGAC CGCACCATTC CATCCGATGC ATGACTGTGG TACATGACAA AGTTTGGTGT
    GGCTATAGGA ACAAAATTTA TGTGGTTCAG CCAAAGGCCA TGAAAATAGA GAAATCATTT
    GATGCACACC CCAGGAAGGA GAGCCAAGTG CGGCAGCTTG CCTGGGTGGG TGATGGCGTG
    TGGGTCTCCA TTCGCTTGGA TTCCACACTC CGTCTCTATC ATGCACACAC TTATCAGCAT
    CTGCAGGATG TGGACATTGA GCCTTATGTA AGCAAAATGC TAGGTACTGG AAAACTGGGC
    TTCTCTTTTG TGAGGATCAC AGCGCTTATG GTGTCTTGTA ACCGTTTGTG GGTGGGAACA
    GGAAATGGTG TCATTATCTC CATCCCATTG ACAGAAACCG TAATCCTCCA CCAGGGACGT
    TTACTGGGGC TGAGGGCGAA TAAAACATCA GGAGCATCGG GAAATCGTCC TGGAAGTGTC
    ATTCGAGTAT ATGGTGATGA AAACAGTGAT AAAGTGACAC CAGGGACATT TATACCCTAT
    TGTTCAATGG CACATGCACA GCTTTGCTTC CATGGGCACA GGGATGCTGT GAAATTTTTT
    GTGGCTGTCC CAGGTCAAGT CATCAGCCCA CAAAGTGGCA GCAGTGGTAC AGATCTGACA
    GTTGACAAAG CAGGGCCATC TGCACAGGAG CCTGGCATCC AGACACCCTT GAAGTCTGTG
    CTTGTCATTA GTGGAGGAGA GGGCTACATT GACTTCCGAA TGGGTGATGA AGGTGGAGAA
    TCAGAGCTTC TTGGAGAGGA TCTTCCACTT GAACCTTCTG TCGCCAAAGC AGAAAGGAGT
    CACTTGATAG TGTGGCAAGT GATGTATGGT AGTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836140.1
    CDS127..3525
    Translation

    Target ORF information:

    RefSeq Version XM_024980372.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTTTGCA TCAGAGACAC ACTGAGAAAA GAACGTCCTA TATCGTTAGG GATTTTCCCT 
    TTACCTTCTG GAGACGGATT GCTTACGCCT GACACTCAGA AAGGTGGCGA GACCCCTGGA
    TCAGAACAAT GGAAATTTCA GGAATTAAGT CAACCACGTT CTCATACCAG TCTGAAGGAT
    GAACTTTCCG ATGTTAGCCA GGGAGGATCT AAAGCCACCA CTCCAGCGTC GACAGCTGCT
    TCAGATGTGG CAGCAACACC TAGCGATACT CCCTTACATG AGGAGAACGG AGGGGTTGTG
    GAGGTTGCAG ATACACCCGA TAAGTCAGAG ATAAGCAAGC ATATCGAAGT ACACGTAGTC
    CAGGAAACTA GAAACATAGC AACGGGTTCT GCTGAAAATG AAGAGAAGTC AGAAGTTCAG
    GCAATCATTG AATCCACTCC TGAGTTGGAT ATGGACAAAG ATCTCAGTGG ATATAAAGGC
    TCAAGCACTC CCACCAAAGG CATAGAGAAC AAAGCTTTTG ATCGCAATAC AGAATCTCTC
    TTTGAAGAAC TGTCTTCAGC TGGTTCAGGT CTAATTGGAG ATGTGGATGA AGGAGCAGAT
    TTACTAGGAA TGGGTCGTGA AGTTGAAAAT CTCATATTGG AAAATACACA ACTATTGGAA
    ACCAAAAATG CCTTGAATGT AGTGAAGAAT GACTTGATAG CAAAAGTAGA TGAACTTACC
    TGTGAGAAAG ATGTGCTGCA AGGGGAGCTG GAGGCTGTGA AACAAGCCAA GCTGAAGCTG
    GAGGAAAAGA ACAAAGAATT GGAAGAAGAG CTTAGGAAAG CTCGTGCAGA AGTTGAAGAT
    GCAAAGCAAA AAGCAAAAGA TGATGATGAT AGTGATATTC CCACAGCCCA AAGGAAGCGG
    TTTACTAGAG TAGAAATGGC CCGAGTTCTA ATGGAGAGAA ACCAATATAA AGAGAGATTG
    ATGGAGCTTC AGGAAGCTGT TCGATGGACA GAGATGATTC GTGCGTCAAG AGAAAATCCA
    GCCATGCAGG AGAAGAAAAG GTCAAGCATT TGGCAGTTTT TCAGCCGACT TTTCAGCTCC
    TCAAGTAACA CTACTAAGAA GCCCGAACCA CCAGTTAATC TGAAGTACAA TGCACCAACC
    TCTCACGTTA CGCCTTCCGT CAAGAAAAGA AGCAGCACCT TATCTCAACT CCCTGGGGAT
    AAGTCCAGAG CCTTTGATTT CCTTAGCGAA GAAACTGAAG CTAGTTTAGC CTCACGCAGA
    GAACAAAAGA GAGAACAGTA TCGTCAGGTA AAGGCCCATG TTCAGAAGGA AGATGGTAGA
    GTGCAGGCTT TTGGCTGGAG TCTGCCTCAG AAATACAAAC AGGTAACCAA CGGACAAGGG
    GAAAATAAGA TGAAAAATTT ACCTGTGCCT GTCTATCTCA GACCTCTAGA TGAAAAAGAT
    GCATCAATGA AGCTTTGGTG TGCTGTTGGA GTCAATTTGT CTGGTGGGAA GACCAGAGAT
    GGTGGTTCTG TTGTTGGAGC AAGTGTGTTT TACAAGGATG TTGCTGGTTT GGAAGCAGAA
    GGCAGTAAAC AGCGAAGTGC ATCTCAGAAT AGCTTAGACA AGTTAGACCA GGAACTTAAG
    GAACAACAGA AGGAGTTAAA AAATCAAGAA GAATTATCCA GTCTAGTCTG GATCTGTACC
    AGCACTCATT CAGCTACAAA AGTTATCATT ATCGATGCTG TTCAACCAGG CAACATCCTA
    GACAGCTTCA CTGTTTGTAA CTCTCACGTT CTGTGTATAT CAAGCGTACC AGGAGCCCGA
    GAAACAGACT ACCCTGCAGG AGAAGAGCTT TCAGAATCTG GACAAGTAGA CAAAGCATCT
    TTATGTGGAA GCATGACAAG CAATAGCTCA GCAGAGACAG ACAGCCTGTT GGGAGGCATC
    ACAGTAGTTG GTTGTTCTGC AGAAGGTGTG ACAGCAGCTG CCACTTCCCC TAATACCAAT
    GAACCTTCTC CAGTGATGGA AAAACCGCCA GAAAAGGAAA CAGAAAATAG TGAGATTGAT
    GAAAATGTTC CAACAGCAGA AGAAGCAACT GAAGCTACAG AAGGCAATGC AGGATCAGCT
    GAAGATACTG TGGATGTCTC CCAAACTGGC GTGTACACAG AGCATGTGTT TACAGATCCT
    TTGGGAGTTC AAATCCCAGA AGACCTGTCA CCAGTATATC AGTCAAGTAA TGACTCAGAT
    GCATATAAAG ATCAAATATC AGTATTGCCA AATGAACAAG ACCTGGTGAG AGAGGAAGCC
    CAGAAAATGA GTAGCCTTTT ACCAACTATG TGGCTTGGAG CTCAAAATGG GTGTTTGTAT
    GTCCATTCAT CTGTAGCCCA GTGGAGGAAA TGTCTCCATT CTATTAAACT TAAAGATTCG
    ATTCTCAGTA TTGTACACGT GAAAGGAATT GTGTTAGTGG CCCTAGCTGA TGGGACCCTT
    GCCATCTTTC ACAGAGGAGT TGATGGACAG TGGGATTTGT CAAACTATCA CCTGTTAGAT
    CTTGGACGAC CGCACCATTC CATCCGATGC ATGACTGTGG TACATGACAA AGTTTGGTGT
    GGCTATAGGA ACAAAATTTA TGTGGTTCAG CCAAAGGCCA TGAAAATAGA GAAATCATTT
    GATGCACACC CCAGGAAGGA GAGCCAAGTG CGGCAGCTTG CCTGGGTGGG TGATGGCGTG
    TGGGTCTCCA TTCGCTTGGA TTCCACACTC CGTCTCTATC ATGCACACAC TTATCAGCAT
    CTGCAGGATG TGGACATTGA GCCTTATGTA AGCAAAATGC TAGGTACTGG AAAACTGGGC
    TTCTCTTTTG TGAGGATCAC AGCGCTTATG GTGTCTTGTA ACCGTTTGTG GGTGGGAACA
    GGAAATGGTG TCATTATCTC CATCCCATTG ACAGAAACCG TAATCCTCCA CCAGGGACGT
    TTACTGGGGC TGAGGGCGAA TAAAACATCA GGAGCATCGG GAAATCGTCC TGGAAGTGTC
    ATTCGAGTAT ATGGTGATGA AAACAGTGAT AAAGTGACAC CAGGGACATT TATACCCTAT
    TGTTCAATGG CACATGCACA GCTTTGCTTC CATGGGCACA GGGATGCTGT GAAATTTTTT
    GTGGCTGTCC CAGGTCAAGT CATCAGCCCA CAAAGTGGCA GCAGTGGTAC AGATCTGACA
    GTTGACAAAG CAGGGCCATC TGCACAGGAG CCTGGCATCC AGACACCCTT GAAGTCTGTG
    CTTGTCATTA GTGGAGGAGA GGGCTACATT GACTTCCGAA TGGGTGATGA AGGTGGAGAA
    TCAGAGCTTC TTGGAGAGGA TCTTCCACTT GAACCTTCTG TCGCCAAAGC AGAAAGGAGT
    CACTTGATAG TGTGGCAAGT GATGTATGGT AGTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39848
    Clone ID Related Accession (Same CDS sequence) NM_001076925.1
    Accession Version NM_001076925.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC123734.1. On or before Oct 11, 2006 this sequence version replaced XM_880305.1, XM_867501.1, XM_880351.1, XM_880378.1, XM_880397.1, XM_612647.2. ##Evidence-Data-START## Transcript exon combination :: BC123734.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145425 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
    AATTCTGCTA TCTTAGTATC TCTCTCCGTC TTGCTGCTTG TGCACTTCTC TATTTCTACC
    GGAGTGCCAG CTTTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG TCCTATATCG
    TTAGGGATTT TCCCTTTACC TTCTGGAGAC GGATTGCTTA CGCCTGACAC TCAGAAAGGT
    GGCGAGACCC CTGGATCAGA ACAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT
    ACCAGTCTGA AGGATGAACT TTCCGATGTT AGCCAGGGAG GATCTAAAGC CACCACTCCA
    GCGTCGACAG CTGCTTCAGA TGTGGCAGCA ACACCTAGCG ATACTCCCTT ACATGAGGAG
    AACGGAGGGG TTGTGGAGGT TGCAGATACA CCCGATAAGT CAGAGATAAG CAAGCATATC
    GAAGTACACG TAGTCCAGGA AACTAGAAAC ATAGCAACGG GTTCTGCTGA AAATGAAGAG
    AAGTCAGAAG TTCAGGCAAT CATTGAATCC ACTCCTGAGT TGGATATGGA CAAAGATCTC
    AGTGGATATA AAGGCTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC
    AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGTT CAGGTCTAAT TGGAGATGTG
    GATGAAGGAG CAGATTTACT AGGAATGGGT CGTGAAGTTG AAAATCTCAT ATTGGAAAAT
    ACACAACTAT TGGAAACCAA AAATGCCTTG AATGTAGTGA AGAATGACTT GATAGCAAAA
    GTAGATGAAC TTACCTGTGA GAAAGATGTG CTGCAAGGGG AGCTGGAGGC TGTGAAACAA
    GCCAAGCTGA AGCTGGAGGA AAAGAACAAA GAATTGGAAG AAGAGCTTAG GAAAGCTCGT
    GCAGAAGTTG AAGATGCAAA GCAAAAAGCA AAAGATGATG ATGATAGTGA TATTCCCACA
    GCCCAAAGGA AGCGGTTTAC TAGAGTAGAA ATGGCCCGAG TTCTAATGGA GAGAAACCAA
    TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGTGCG
    TCAAGAGAAA ATCCAGCCAT GCAGGAGAAG AAAAGGTCAA GCATTTGGCA GTTTTTCAGC
    CGACTTTTCA GCTCCTCAAG TAACACTACT AAGAAGCCCG AACCACCAGT TAATCTGAAG
    TACAATGCAC CAACCTCTCA CGTTACGCCT TCCGTCAAGA AAAGAAGCAG CACCTTATCT
    CAACTCCCTG GGGATAAGTC CAGAGCCTTT GATTTCCTTA GCGAAGAAAC TGAAGCTAGT
    TTAGCCTCAC GGAGAGAACA AAAGAGAGAA CAGTATCGTC AGGTAAAGGC CCATGTTCAG
    AAGGAAGATG GTAGAGTGCA GGCTTTTGGC TGGAGTCTGC CTCAGAAATA CAAACAGGTA
    ACCAACGGAC AAGGGGAAAA TAAGATGAAA AATTTACCTG TGCCTGTCTA TCTCAGACCT
    CTAGATGAAA AAGATGCATC AATGAAGCTT TGGTGTGCTG TTGGAGTCAA TTTGTCTGGT
    GGGAAGACCA GAGATGGTGG TTCTGTTGTT GGAGCAAGTG TGTTTTACAA GGATGTTGCT
    GGTTTGGAAG CAGAAGGCAG TAAACAGCGA AGTGCATCTC AGAATAGCTT AGACAAGTTA
    GACCAGGAAC TTAAGGAACA ACAGAAGGAG TTAAAAAATC AAGAAGAATT ATCCAGTCTA
    GTCTGGATCT GTACCAGCAC TCATTCAGCT ACAAAAGTTA TCATTATCGA TGCTGTTCAA
    CCAGGCAACA TCCTAGACAG CTTCACTGTT TGTAACTCTC ACGTTCTGTG TGTATCAAGC
    GTACCAGGAG CCCGAGAAAC AGACTACCCT GCAGGAGAAG AGCTTTCAGA ATCTGGACAA
    GTAGACAAAG CATCTTTATG TGGAAGCATG ACAAGCAATA GCTCAGCAGA GACAGACAGC
    CTGTTGGGAG GCATCACAGT AGTTGGTTGT TCTGCAGAAG GTGTGACAGC AGCTGCCACT
    TCCCCTAATA CCAATGAACC TTCTCCAGTG ATGGAAAAAC CGCCAGAAAA GGAAACAGAA
    AATAGTGAGA TTGATGAAAA TGTTCCAACA GCAGAAGAAG CAACTGAAGC TACAGAAGGC
    AATGCAGGAT CAGCTGAAGA TACTGTGGAT GTCTCCCAAA CTGGCGTGTA CACAGAGCAT
    GTGTTTACAG ATCCTTTGGG AGTTCAAATC CCAGAAGACC TGTCACCAGT ATATCAGTCA
    AGTAATGACT CAGATGCATA TAAAGATCAA ATATCAGTAT TGCCAAATGA ACAAGACCTG
    GTGAGAGAGG AAGCCCAGAA AATGAGTAGC CTTTTACCAA CTATGTGGCT TGGAGCTCAA
    AATGGGTGTT TGTATGTCCA TTCATCTGTA GCCCAGTGGA GGAAATGTCT CCATTCTATT
    AAACTTAAAG ATTCGATTCT CAGTATTGTA CACGTGAAAG GAATTGTGTT AGTGGCCCTA
    GCTGATGGGA CCCTTGCCAT CTTTCACAGA GGAGTTGATG GACAGTGGGA TTTGTCAAAC
    TATCACCTGT TAGATCTTGG ACGACCGCAC CATTCCATCC GATGCATGAC TGTGGTACAT
    GACAAAGTTT GGTGTGGCTA TAGGAACAAA ATTTATGTGG TTCAGCCAAA GGCCATGAAA
    ATAGAGAAAT CATTTGATGC ACACCCCAGG AAGGAGAGCC AAGTGCGGCA GCTTGCCTGG
    GTGGGTGATG GCGTGTGGGT CTCCATTCGC TTGGATTCCA CACTCCGTCT CTATCATGCA
    CACACTTATC AGCATCTGCA GGATGTGGAC ATTGAGCCTT ATGTAAGCAA AATGCTAGGT
    ACTGGAAAAC TGGGCTTCTC TTTTGTGAGG ATCACAGCGC TTATGGTGTC TTGTAACCGT
    TTGTGGGTGG GAACAGGAAA TGGTGTCATT ATCTCCATCC CATTGACAGA AACCGTAATC
    CTCCACCAGG GACGTTTACT GGGGCTGAGG GCGAATAAAA CATCAGGAGC ATCGGGAAAT
    CGTCCTGGAA GTGTCATTCG AGTATATGGT GATGAAAACA GTGATAAAGT GACACCAGGG
    ACATTTATAC CCTATTGTTC AATGGCACAT GCACAGCTTT GCTTCCATGG GCACAGGGAT
    GCTGTGAAAT TTTTTGTGGC TGTCCCAGGT CAAGTCATCA GCCCACAAAG TGGCAGCAGT
    GGTACAGATC TGACAGTTGA CAAAGCAGGG CCATCTGCAC AGGAGCCTGG CATCCAGACA
    CCCTTGAAGT CTGTGCTTGT CATTAGTGGA GGAGAGGGCT ACATTGACTT CCGAATGGGT
    GATGAAGGTG GAGAATCAGA GCTTCTTGGA GAGGATCTTC CACTTGAACC TTCTGTCGCC
    AAAGCAGAAA GGAGTCACTT GATAGTGTGG CAAGTGATGT ATGGTAGTGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001070393.1
    CDS71..3604
    Translation

    Target ORF information:

    RefSeq Version NM_001076925.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001076925.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
    AATTCTGCTA TCTTAGTATC TCTCTCCGTC TTGCTGCTTG TGCACTTCTC TATTTCTACC
    GGAGTGCCAG CTTTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG TCCTATATCG
    TTAGGGATTT TCCCTTTACC TTCTGGAGAC GGATTGCTTA CGCCTGACAC TCAGAAAGGT
    GGCGAGACCC CTGGATCAGA ACAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT
    ACCAGTCTGA AGGATGAACT TTCCGATGTT AGCCAGGGAG GATCTAAAGC CACCACTCCA
    GCGTCGACAG CTGCTTCAGA TGTGGCAGCA ACACCTAGCG ATACTCCCTT ACATGAGGAG
    AACGGAGGGG TTGTGGAGGT TGCAGATACA CCCGATAAGT CAGAGATAAG CAAGCATATC
    GAAGTACACG TAGTCCAGGA AACTAGAAAC ATAGCAACGG GTTCTGCTGA AAATGAAGAG
    AAGTCAGAAG TTCAGGCAAT CATTGAATCC ACTCCTGAGT TGGATATGGA CAAAGATCTC
    AGTGGATATA AAGGCTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC
    AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGTT CAGGTCTAAT TGGAGATGTG
    GATGAAGGAG CAGATTTACT AGGAATGGGT CGTGAAGTTG AAAATCTCAT ATTGGAAAAT
    ACACAACTAT TGGAAACCAA AAATGCCTTG AATGTAGTGA AGAATGACTT GATAGCAAAA
    GTAGATGAAC TTACCTGTGA GAAAGATGTG CTGCAAGGGG AGCTGGAGGC TGTGAAACAA
    GCCAAGCTGA AGCTGGAGGA AAAGAACAAA GAATTGGAAG AAGAGCTTAG GAAAGCTCGT
    GCAGAAGTTG AAGATGCAAA GCAAAAAGCA AAAGATGATG ATGATAGTGA TATTCCCACA
    GCCCAAAGGA AGCGGTTTAC TAGAGTAGAA ATGGCCCGAG TTCTAATGGA GAGAAACCAA
    TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGTGCG
    TCAAGAGAAA ATCCAGCCAT GCAGGAGAAG AAAAGGTCAA GCATTTGGCA GTTTTTCAGC
    CGACTTTTCA GCTCCTCAAG TAACACTACT AAGAAGCCCG AACCACCAGT TAATCTGAAG
    TACAATGCAC CAACCTCTCA CGTTACGCCT TCCGTCAAGA AAAGAAGCAG CACCTTATCT
    CAACTCCCTG GGGATAAGTC CAGAGCCTTT GATTTCCTTA GCGAAGAAAC TGAAGCTAGT
    TTAGCCTCAC GGAGAGAACA AAAGAGAGAA CAGTATCGTC AGGTAAAGGC CCATGTTCAG
    AAGGAAGATG GTAGAGTGCA GGCTTTTGGC TGGAGTCTGC CTCAGAAATA CAAACAGGTA
    ACCAACGGAC AAGGGGAAAA TAAGATGAAA AATTTACCTG TGCCTGTCTA TCTCAGACCT
    CTAGATGAAA AAGATGCATC AATGAAGCTT TGGTGTGCTG TTGGAGTCAA TTTGTCTGGT
    GGGAAGACCA GAGATGGTGG TTCTGTTGTT GGAGCAAGTG TGTTTTACAA GGATGTTGCT
    GGTTTGGAAG CAGAAGGCAG TAAACAGCGA AGTGCATCTC AGAATAGCTT AGACAAGTTA
    GACCAGGAAC TTAAGGAACA ACAGAAGGAG TTAAAAAATC AAGAAGAATT ATCCAGTCTA
    GTCTGGATCT GTACCAGCAC TCATTCAGCT ACAAAAGTTA TCATTATCGA TGCTGTTCAA
    CCAGGCAACA TCCTAGACAG CTTCACTGTT TGTAACTCTC ACGTTCTGTG TGTATCAAGC
    GTACCAGGAG CCCGAGAAAC AGACTACCCT GCAGGAGAAG AGCTTTCAGA ATCTGGACAA
    GTAGACAAAG CATCTTTATG TGGAAGCATG ACAAGCAATA GCTCAGCAGA GACAGACAGC
    CTGTTGGGAG GCATCACAGT AGTTGGTTGT TCTGCAGAAG GTGTGACAGC AGCTGCCACT
    TCCCCTAATA CCAATGAACC TTCTCCAGTG ATGGAAAAAC CGCCAGAAAA GGAAACAGAA
    AATAGTGAGA TTGATGAAAA TGTTCCAACA GCAGAAGAAG CAACTGAAGC TACAGAAGGC
    AATGCAGGAT CAGCTGAAGA TACTGTGGAT GTCTCCCAAA CTGGCGTGTA CACAGAGCAT
    GTGTTTACAG ATCCTTTGGG AGTTCAAATC CCAGAAGACC TGTCACCAGT ATATCAGTCA
    AGTAATGACT CAGATGCATA TAAAGATCAA ATATCAGTAT TGCCAAATGA ACAAGACCTG
    GTGAGAGAGG AAGCCCAGAA AATGAGTAGC CTTTTACCAA CTATGTGGCT TGGAGCTCAA
    AATGGGTGTT TGTATGTCCA TTCATCTGTA GCCCAGTGGA GGAAATGTCT CCATTCTATT
    AAACTTAAAG ATTCGATTCT CAGTATTGTA CACGTGAAAG GAATTGTGTT AGTGGCCCTA
    GCTGATGGGA CCCTTGCCAT CTTTCACAGA GGAGTTGATG GACAGTGGGA TTTGTCAAAC
    TATCACCTGT TAGATCTTGG ACGACCGCAC CATTCCATCC GATGCATGAC TGTGGTACAT
    GACAAAGTTT GGTGTGGCTA TAGGAACAAA ATTTATGTGG TTCAGCCAAA GGCCATGAAA
    ATAGAGAAAT CATTTGATGC ACACCCCAGG AAGGAGAGCC AAGTGCGGCA GCTTGCCTGG
    GTGGGTGATG GCGTGTGGGT CTCCATTCGC TTGGATTCCA CACTCCGTCT CTATCATGCA
    CACACTTATC AGCATCTGCA GGATGTGGAC ATTGAGCCTT ATGTAAGCAA AATGCTAGGT
    ACTGGAAAAC TGGGCTTCTC TTTTGTGAGG ATCACAGCGC TTATGGTGTC TTGTAACCGT
    TTGTGGGTGG GAACAGGAAA TGGTGTCATT ATCTCCATCC CATTGACAGA AACCGTAATC
    CTCCACCAGG GACGTTTACT GGGGCTGAGG GCGAATAAAA CATCAGGAGC ATCGGGAAAT
    CGTCCTGGAA GTGTCATTCG AGTATATGGT GATGAAAACA GTGATAAAGT GACACCAGGG
    ACATTTATAC CCTATTGTTC AATGGCACAT GCACAGCTTT GCTTCCATGG GCACAGGGAT
    GCTGTGAAAT TTTTTGTGGC TGTCCCAGGT CAAGTCATCA GCCCACAAAG TGGCAGCAGT
    GGTACAGATC TGACAGTTGA CAAAGCAGGG CCATCTGCAC AGGAGCCTGG CATCCAGACA
    CCCTTGAAGT CTGTGCTTGT CATTAGTGGA GGAGAGGGCT ACATTGACTT CCGAATGGGT
    GATGAAGGTG GAGAATCAGA GCTTCTTGGA GAGGATCTTC CACTTGAACC TTCTGTCGCC
    AAAGCAGAAA GGAGTCACTT GATAGTGTGG CAAGTGATGT ATGGTAGTGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29946
    Clone ID Related Accession (Same CDS sequence) XM_005220611.3
    Accession Version XM_005220611.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220611.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACTACTA AGAAGCCCGA ACCACCAGTT
    AATCTGAAGT ACAATGCACC AACCTCTCAC GTTACGCCTT CCGTCAAGAA AAGAAGCAGC
    ACCTTATCTC AACTCCCTGG GGATAAGTCC AGAGCCTTTG ATTTCCTTAG CGAAGAAACT
    GAAGCTAGTT TAGCCTCACG GAGAGAACAA AAGAGAGAAC AGTATCGTCA GGTAAAGGCC
    CATGTTCAGA AGGAAGATGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAATAC
    AAACAGGTAA CCAACGGACA AGGGGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT
    CTCAGACCTC TAGATGAAAA AGATGCATCA ATGAAGCTTT GGTGTGCTGT TGGAGTCAAT
    TTGTCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT GTTTTACAAG
    GATGTTGCTG GTTTGGAAGC AGAAGGCAGT AAACAGCGAA GTGCATCTCA GAATAGCTTA
    GACAAGTTAG ACCAGGAACT TAAGGAACAA CAGAAGGAGT TAAAAAATCA AGAAGAATTA
    TCCAGTCTAG TCTGGATCTG TACCAGCACT CATTCAGCTA CAAAAGTTAT CATTATCGAT
    GCTGTTCAAC CAGGCAACAT CCTAGACAGC TTCACTGTTT GTAACTCTCA CGTTCTGTGT
    ATATCAAGCG TACCAGGAGC CCGAGAAACA GACTACCCTG CAGGAGAAGA GCTTTCAGAA
    TCTGGACAAG TAGACAAAGC ATCTTTATGT GGAAGCATGA CAAGCAATAG CTCAGCAGAG
    ACAGACAGCC TGTTGGGAGG CATCACAGTA GTTGGTTGTT CTGCAGAAGG TGTGACAGCA
    GCTGCCACTT CCCCTAATAC CAATGAACCT TCTCCAGTGA TGGAAAAACC GCCAGAAAAG
    GAAACAGAAA ATAGTGAGAT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT
    ACAGAAGGCA ATGCAGGATC AGCTGAAGAT ACTGTGGATG TCTCCCAAAC TGGCGTGTAC
    ACAGAGCATG TGTTTACAGA TCCTTTGGGA GTTCAAATCC CAGAAGACCT GTCACCAGTA
    TATCAGTCAA GTAATGACTC AGATGCATAT AAAGATCAAA TATCAGTATT GCCAAATGAA
    CAAGACCTGG TGAGAGAGGA AGCCCAGAAA ATGAGTAGCC TTTTACCAAC TATGTGGCTT
    GGAGCTCAAA ATGGGTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC
    CATTCTATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAAGG AATTGTGTTA
    GTGGCCCTAG CTGATGGGAC CCTTGCCATC TTTCACAGAG GAGTTGATGG ACAGTGGGAT
    TTGTCAAACT ATCACCTGTT AGATCTTGGA CGACCGCACC ATTCCATCCG ATGCATGACT
    GTGGTACATG ACAAAGTTTG GTGTGGCTAT AGGAACAAAA TTTATGTGGT TCAGCCAAAG
    GCCATGAAAA TAGAGAAATC ATTTGATGCA CACCCCAGGA AGGAGAGCCA AGTGCGGCAG
    CTTGCCTGGG TGGGTGATGG TGTGTGGGTC TCCATTCGCT TGGATTCCAC ACTCCGTCTC
    TATCATGCAC ACACTTATCA GCATCTGCAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA
    ATGCTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGGA TCACAGCGCT TATGGTGTCT
    TGTAACCGTT TGTGGGTGGG AACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA
    ACCGTAATCC TCCACCAGGG ACGTTTACTG GGGCTGAGGG CGAATAAAAC ATCAGGAGCA
    TCGGGAAATC GTCCTGGAAG TGTCATTCGA GTATATGGTG ATGAAAACAG TGATAAAGTG
    ACACCAGGGA CATTTATACC CTATTGTTCA ATGGCACATG CACAGCTTTG CTTCCATGGG
    CACAGGGATG CTGTGAAATT TTTTGTGGCT GTCCCAGGTC AAGTCATCAG CCCACAAAGT
    GGCAGCAGTG GTACAGATCT GACAGTTGAC AAAGCAGGGC CATCTGCACA GGAGCCTGGC
    ATCCAGACAC CCTTGAAGTC TGTGCTTGTC ATTAGTGGAG GAGAGGGCTA CATTGACTTC
    CGAATGGGTG ATGAAGGTGG AGAATCAGAG CTTCTTGGAG AGGATCTTCC ACTTGAACCT
    TCTGTCGCCA AAGCAGAAAG GAGTCACTTG ATAGTGTGGC AAGTGATGTA TGGTAGTGAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220668.1
    CDS1..3963
    Translation

    Target ORF information:

    RefSeq Version XM_005220611.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220611.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACTACTA AGAAGCCCGA ACCACCAGTT
    AATCTGAAGT ACAATGCACC AACCTCTCAC GTTACGCCTT CCGTCAAGAA AAGAAGCAGC
    ACCTTATCTC AACTCCCTGG GGATAAGTCC AGAGCCTTTG ATTTCCTTAG CGAAGAAACT
    GAAGCTAGTT TAGCCTCACG GAGAGAACAA AAGAGAGAAC AGTATCGTCA GGTAAAGGCC
    CATGTTCAGA AGGAAGATGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAATAC
    AAACAGGTAA CCAACGGACA AGGGGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT
    CTCAGACCTC TAGATGAAAA AGATGCATCA ATGAAGCTTT GGTGTGCTGT TGGAGTCAAT
    TTGTCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT GTTTTACAAG
    GATGTTGCTG GTTTGGAAGC AGAAGGCAGT AAACAGCGAA GTGCATCTCA GAATAGCTTA
    GACAAGTTAG ACCAGGAACT TAAGGAACAA CAGAAGGAGT TAAAAAATCA AGAAGAATTA
    TCCAGTCTAG TCTGGATCTG TACCAGCACT CATTCAGCTA CAAAAGTTAT CATTATCGAT
    GCTGTTCAAC CAGGCAACAT CCTAGACAGC TTCACTGTTT GTAACTCTCA CGTTCTGTGT
    ATATCAAGCG TACCAGGAGC CCGAGAAACA GACTACCCTG CAGGAGAAGA GCTTTCAGAA
    TCTGGACAAG TAGACAAAGC ATCTTTATGT GGAAGCATGA CAAGCAATAG CTCAGCAGAG
    ACAGACAGCC TGTTGGGAGG CATCACAGTA GTTGGTTGTT CTGCAGAAGG TGTGACAGCA
    GCTGCCACTT CCCCTAATAC CAATGAACCT TCTCCAGTGA TGGAAAAACC GCCAGAAAAG
    GAAACAGAAA ATAGTGAGAT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT
    ACAGAAGGCA ATGCAGGATC AGCTGAAGAT ACTGTGGATG TCTCCCAAAC TGGCGTGTAC
    ACAGAGCATG TGTTTACAGA TCCTTTGGGA GTTCAAATCC CAGAAGACCT GTCACCAGTA
    TATCAGTCAA GTAATGACTC AGATGCATAT AAAGATCAAA TATCAGTATT GCCAAATGAA
    CAAGACCTGG TGAGAGAGGA AGCCCAGAAA ATGAGTAGCC TTTTACCAAC TATGTGGCTT
    GGAGCTCAAA ATGGGTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC
    CATTCTATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAAGG AATTGTGTTA
    GTGGCCCTAG CTGATGGGAC CCTTGCCATC TTTCACAGAG GAGTTGATGG ACAGTGGGAT
    TTGTCAAACT ATCACCTGTT AGATCTTGGA CGACCGCACC ATTCCATCCG ATGCATGACT
    GTGGTACATG ACAAAGTTTG GTGTGGCTAT AGGAACAAAA TTTATGTGGT TCAGCCAAAG
    GCCATGAAAA TAGAGAAATC ATTTGATGCA CACCCCAGGA AGGAGAGCCA AGTGCGGCAG
    CTTGCCTGGG TGGGTGATGG TGTGTGGGTC TCCATTCGCT TGGATTCCAC ACTCCGTCTC
    TATCATGCAC ACACTTATCA GCATCTGCAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA
    ATGCTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGGA TCACAGCGCT TATGGTGTCT
    TGTAACCGTT TGTGGGTGGG AACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA
    ACCGTAATCC TCCACCAGGG ACGTTTACTG GGGCTGAGGG CGAATAAAAC ATCAGGAGCA
    TCGGGAAATC GTCCTGGAAG TGTCATTCGA GTATATGGTG ATGAAAACAG TGATAAAGTG
    ACACCAGGGA CATTTATACC CTATTGTTCA ATGGCACATG CACAGCTTTG CTTCCATGGG
    CACAGGGATG CTGTGAAATT TTTTGTGGCT GTCCCAGGTC AAGTCATCAG CCCACAAAGT
    GGCAGCAGTG GTACAGATCT GACAGTTGAC AAAGCAGGGC CATCTGCACA GGAGCCTGGC
    ATCCAGACAC CCTTGAAGTC TGTGCTTGTC ATTAGTGGAG GAGAGGGCTA CATTGACTTC
    CGAATGGGTG ATGAAGGTGG AGAATCAGAG CTTCTTGGAG AGGATCTTCC ACTTGAACCT
    TCTGTCGCCA AAGCAGAAAG GAGTCACTTG ATAGTGTGGC AAGTGATGTA TGGTAGTGAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29950
    Clone ID Related Accession (Same CDS sequence) XM_005220615.3
    Accession Version XM_005220615.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220615.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACTACTA AGAAGCCCGA ACCACCAGTT
    AATCTGAAGT ACAATGCACC AACCTCTCAC GTTACGCCTT CCGTCAAGAA AAGAAGCAGC
    ACCTTATCTC AACTCCCTGG GGATAAGTCC AGAGCCTTTG ATTTCCTTAG CGAAGAAACT
    GAAGCTAGTT TAGCCTCACG GAGAGAACAA AAGAGAGAAC AGTATCGTCA GGTAAAGGCC
    CATGTTCAGA AGGAAGATGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAATAC
    AAACAGGTAA CCAACGGACA AGGGGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT
    CTCAGACCTC TAGATGAAAA AGATGCATCA ATGAAGCTTT GGTGTGCTGT TGGAGTCAAT
    TTGTCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT GTTTTACAAG
    GATGTTGCTG GTTTGGAAGC AGAAGGCAGT AAACAGCGAA GTGCATCTCA GAATAGCTTA
    GACAAGTTAG ACCAGGAACT TAAGGAACAA CAGAAGGAGT TAAAAAATCA AGAAGAATTA
    TCCAGTCTAG TCTGGATCTG TACCAGCACT CATTCAGCTA CAAAAGTTAT CATTATCGAT
    GCTGTTCAAC CAGGCAACAT CCTAGACAGC TTCACTGTTT GTAACTCTCA CGTTCTGTGT
    ATATCAAGCG TACCAGGAGC CCGAGAAACA GACTACCCTG CAGGAGAAGA GCTTTCAGAA
    TCTGGACAAG TAGACAAAGC ATCTTTATGT GGAAGCATGA CAAGCAATAG CTCAGCAGAG
    ACAGACAGCC TGTTGGGAGG CATCACAGTA GTTGGTTGTT CTGCAGAAGG TGTGACAGCA
    GCTGCCACTT CCCCTAATAC CAATGAACCT TCTCCAGTGA TGGAAAAACC GCCAGAAAAG
    GAAACAGAAA ATAGTGAGAT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT
    ACAGAAGGCA ATGCAGGATC AGCTGAAGAT ACTGTGGATG TCTCCCAAAC TGGCGTGTAC
    ACAGAGCATG TGTTTACAGA TCCTTTGGGA GTTCAAATCC CAGAAGACCT GTCACCAGTA
    TATCAGTCAA GTAATGACTC AGATGCATAT AAAGATCAAA TATCAGTATT GCCAAATGAA
    CAAGACCTGG TGAGAGAGGA AGCCCAGAAA ATGAGTAGCC TTTTACCAAC TATGTGGCTT
    GGAGCTCAAA ATGGGTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC
    CATTCTATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAAGG AATTGTGTTA
    GTGGCCCTAG CTGATGGGAC CCTTGCCATC TTTCACAGAG GAGTTGATGG ACAGTGGGAT
    TTGTCAAACT ATCACCTGTT AGATCTTGGA CGACCGCACC ATTCCATCCG ATGCATGACT
    GTGGTACATG ACAAAGTTTG GTGTGGCTAT AGGAACAAAA TTTATGTGGT TCAGCCAAAG
    GCCATGAAAA TAGAGAAATC ATTTGATGCA CACCCCAGGA AGGAGAGCCA AGTGCGGCAG
    CTTGCCTGGG TGGGTGATGG TGTGTGGGTC TCCATTCGCT TGGATTCCAC ACTCCGTCTC
    TATCATGCAC ACACTTATCA GCATCTGCAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA
    ATGCTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGGA TCACAGCGCT TATGGTGTCT
    TGTAACCGTT TGTGGGTGGG AACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA
    ACGAATAAAA CATCAGGAGC ATCGGGAAAT CGTCCTGGAA GTGTCATTCG AGTATATGGT
    GATGAAAACA GTGATAAAGT GACACCAGGG ACATTTATAC CCTATTGTTC AATGGCACAT
    GCACAGCTTT GCTTCCATGG GCACAGGGAT GCTGTGAAAT TTTTTGTGGC TGTCCCAGGT
    CAAGTCATCA GCCCACAAAG TGGCAGCAGT GGTACAGATC TGACAGTTGA CAAAGCAGGG
    CCATCTGCAC AGGAGCCTGG CATCCAGACA CCCTTGAAGT CTGTGCTTGT CATTAGTGGA
    GGAGAGGGCT ACATTGACTT CCGAATGGGT GATGAAGGTG GAGAATCAGA GCTTCTTGGA
    GAGGATCTTC CACTTGAACC TTCTGTCGCC AAAGCAGAAA GGAGTCACTT GATAGTGTGG
    CAAGTGATGT ATGGTAGTGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220672.1
    CDS1..3924
    Translation

    Target ORF information:

    RefSeq Version XM_005220615.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220615.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACTACTA AGAAGCCCGA ACCACCAGTT
    AATCTGAAGT ACAATGCACC AACCTCTCAC GTTACGCCTT CCGTCAAGAA AAGAAGCAGC
    ACCTTATCTC AACTCCCTGG GGATAAGTCC AGAGCCTTTG ATTTCCTTAG CGAAGAAACT
    GAAGCTAGTT TAGCCTCACG GAGAGAACAA AAGAGAGAAC AGTATCGTCA GGTAAAGGCC
    CATGTTCAGA AGGAAGATGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAATAC
    AAACAGGTAA CCAACGGACA AGGGGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT
    CTCAGACCTC TAGATGAAAA AGATGCATCA ATGAAGCTTT GGTGTGCTGT TGGAGTCAAT
    TTGTCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT GTTTTACAAG
    GATGTTGCTG GTTTGGAAGC AGAAGGCAGT AAACAGCGAA GTGCATCTCA GAATAGCTTA
    GACAAGTTAG ACCAGGAACT TAAGGAACAA CAGAAGGAGT TAAAAAATCA AGAAGAATTA
    TCCAGTCTAG TCTGGATCTG TACCAGCACT CATTCAGCTA CAAAAGTTAT CATTATCGAT
    GCTGTTCAAC CAGGCAACAT CCTAGACAGC TTCACTGTTT GTAACTCTCA CGTTCTGTGT
    ATATCAAGCG TACCAGGAGC CCGAGAAACA GACTACCCTG CAGGAGAAGA GCTTTCAGAA
    TCTGGACAAG TAGACAAAGC ATCTTTATGT GGAAGCATGA CAAGCAATAG CTCAGCAGAG
    ACAGACAGCC TGTTGGGAGG CATCACAGTA GTTGGTTGTT CTGCAGAAGG TGTGACAGCA
    GCTGCCACTT CCCCTAATAC CAATGAACCT TCTCCAGTGA TGGAAAAACC GCCAGAAAAG
    GAAACAGAAA ATAGTGAGAT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT
    ACAGAAGGCA ATGCAGGATC AGCTGAAGAT ACTGTGGATG TCTCCCAAAC TGGCGTGTAC
    ACAGAGCATG TGTTTACAGA TCCTTTGGGA GTTCAAATCC CAGAAGACCT GTCACCAGTA
    TATCAGTCAA GTAATGACTC AGATGCATAT AAAGATCAAA TATCAGTATT GCCAAATGAA
    CAAGACCTGG TGAGAGAGGA AGCCCAGAAA ATGAGTAGCC TTTTACCAAC TATGTGGCTT
    GGAGCTCAAA ATGGGTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC
    CATTCTATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAAGG AATTGTGTTA
    GTGGCCCTAG CTGATGGGAC CCTTGCCATC TTTCACAGAG GAGTTGATGG ACAGTGGGAT
    TTGTCAAACT ATCACCTGTT AGATCTTGGA CGACCGCACC ATTCCATCCG ATGCATGACT
    GTGGTACATG ACAAAGTTTG GTGTGGCTAT AGGAACAAAA TTTATGTGGT TCAGCCAAAG
    GCCATGAAAA TAGAGAAATC ATTTGATGCA CACCCCAGGA AGGAGAGCCA AGTGCGGCAG
    CTTGCCTGGG TGGGTGATGG TGTGTGGGTC TCCATTCGCT TGGATTCCAC ACTCCGTCTC
    TATCATGCAC ACACTTATCA GCATCTGCAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA
    ATGCTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGGA TCACAGCGCT TATGGTGTCT
    TGTAACCGTT TGTGGGTGGG AACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA
    ACGAATAAAA CATCAGGAGC ATCGGGAAAT CGTCCTGGAA GTGTCATTCG AGTATATGGT
    GATGAAAACA GTGATAAAGT GACACCAGGG ACATTTATAC CCTATTGTTC AATGGCACAT
    GCACAGCTTT GCTTCCATGG GCACAGGGAT GCTGTGAAAT TTTTTGTGGC TGTCCCAGGT
    CAAGTCATCA GCCCACAAAG TGGCAGCAGT GGTACAGATC TGACAGTTGA CAAAGCAGGG
    CCATCTGCAC AGGAGCCTGG CATCCAGACA CCCTTGAAGT CTGTGCTTGT CATTAGTGGA
    GGAGAGGGCT ACATTGACTT CCGAATGGGT GATGAAGGTG GAGAATCAGA GCTTCTTGGA
    GAGGATCTTC CACTTGAACC TTCTGTCGCC AAAGCAGAAA GGAGTCACTT GATAGTGTGG
    CAAGTGATGT ATGGTAGTGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29951
    Clone ID Related Accession (Same CDS sequence) XM_005220616.3
    Accession Version XM_005220616.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220616.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTATGCCAG CTCGTTTCAG CCGACTTTTC AGCTCCTCAA GTAACACTAC TAAGAAGCCC
    GAACCACCAG TTAATCTGAA GTACAATGCA CCAACCTCTC ACGTTACGCC TTCCGTCAAG
    AAAAGAAGCA GCACCTTATC TCAACTCCCT GGGGATAAGT CCAGAGCCTT TGATTTCCTT
    AGCGAAGAAA CTGAAGCTAG TTTAGCCTCA CGGAGAGAAC AAAAGAGAGA ACAGTATCGT
    CAGGTAAAGG CCCATGTTCA GAAGGAAGAT GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG
    CCTCAGAAAT ACAAACAGGT AACCAACGGA CAAGGGGAAA ATAAGATGAA AAATTTACCT
    GTGCCTGTCT ATCTCAGACC TCTAGATGAA AAAGATGCAT CAATGAAGCT TTGGTGTGCT
    GTTGGAGTCA ATTTGTCTGG TGGGAAGACC AGAGATGGTG GTTCTGTTGT TGGAGCAAGT
    GTGTTTTACA AGGATGTTGC TGGTTTGGAA GCAGAAGGCA GTAAACAGCG AAGTGCATCT
    CAGAATAGCT TAGACAAGTT AGACCAGGAA CTTAAGGAAC AACAGAAGGA GTTAAAAAAT
    CAAGAAGAAT TATCCAGTCT AGTCTGGATC TGTACCAGCA CTCATTCAGC TACAAAAGTT
    ATCATTATCG ATGCTGTTCA ACCAGGCAAC ATCCTAGACA GCTTCACTGT TTGTAACTCT
    CACGTTCTGT GTATATCAAG CGTACCAGGA GCCCGAGAAA CAGACTACCC TGCAGGAGAA
    GAGCTTTCAG AATCTGGACA AGTAGACAAA GCATCTTTAT GTGGAAGCAT GACAAGCAAT
    AGCTCAGCAG AGACAGACAG CCTGTTGGGA GGCATCACAG TAGTTGGTTG TTCTGCAGAA
    GGTGTGACAG CAGCTGCCAC TTCCCCTAAT ACCAATGAAC CTTCTCCAGT GATGGAAAAA
    CCGCCAGAAA AGGAAACAGA AAATAGTGAG ATTGATGAAA ATGTTCCAAC AGCAGAAGAA
    GCAACTGAAG CTACAGAAGG CAATGCAGGA TCAGCTGAAG ATACTGTGGA TGTCTCCCAA
    ACTGGCGTGT ACACAGAGCA TGTGTTTACA GATCCTTTGG GAGTTCAAAT CCCAGAAGAC
    CTGTCACCAG TATATCAGTC AAGTAATGAC TCAGATGCAT ATAAAGATCA AATATCAGTA
    TTGCCAAATG AACAAGACCT GGTGAGAGAG GAAGCCCAGA AAATGAGTAG CCTTTTACCA
    ACTATGTGGC TTGGAGCTCA AAATGGGTGT TTGTATGTCC ATTCATCTGT AGCCCAGTGG
    AGGAAATGTC TCCATTCTAT TAAACTTAAA GATTCGATTC TCAGTATTGT ACACGTGAAA
    GGAATTGTGT TAGTGGCCCT AGCTGATGGG ACCCTTGCCA TCTTTCACAG AGGAGTTGAT
    GGACAGTGGG ATTTGTCAAA CTATCACCTG TTAGATCTTG GACGACCGCA CCATTCCATC
    CGATGCATGA CTGTGGTACA TGACAAAGTT TGGTGTGGCT ATAGGAACAA AATTTATGTG
    GTTCAGCCAA AGGCCATGAA AATAGAGAAA TCATTTGATG CACACCCCAG GAAGGAGAGC
    CAAGTGCGGC AGCTTGCCTG GGTGGGTGAT GGTGTGTGGG TCTCCATTCG CTTGGATTCC
    ACACTCCGTC TCTATCATGC ACACACTTAT CAGCATCTGC AGGATGTGGA CATTGAGCCT
    TATGTAAGCA AAATGCTAGG TACTGGAAAA CTGGGCTTCT CTTTTGTGAG GATCACAGCG
    CTTATGGTGT CTTGTAACCG TTTGTGGGTG GGAACAGGAA ATGGTGTCAT TATCTCCATC
    CCATTGACAG AAACGAATAA AACATCAGGA GCATCGGGAA ATCGTCCTGG AAGTGTCATT
    CGAGTATATG GTGATGAAAA CAGTGATAAA GTGACACCAG GGACATTTAT ACCCTATTGT
    TCAATGGCAC ATGCACAGCT TTGCTTCCAT GGGCACAGGG ATGCTGTGAA ATTTTTTGTG
    GCTGTCCCAG GTCAAGTCAT CAGCCCACAA AGTGGCAGCA GTGGTACAGA TCTGACAGTT
    GACAAAGCAG GGCCATCTGC ACAGGAGCCT GGCATCCAGA CACCCTTGAA GTCTGTGCTT
    GTCATTAGTG GAGGAGAGGG CTACATTGAC TTCCGAATGG GTGATGAAGG TGGAGAATCA
    GAGCTTCTTG GAGAGGATCT TCCACTTGAA CCTTCTGTCG CCAAAGCAGA AAGGAGTCAC
    TTGATAGTGT GGCAAGTGAT GTATGGTAGT GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220673.1
    CDS1..3936
    Translation

    Target ORF information:

    RefSeq Version XM_005220616.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220616.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTATGCCAG CTCGTTTCAG CCGACTTTTC AGCTCCTCAA GTAACACTAC TAAGAAGCCC
    GAACCACCAG TTAATCTGAA GTACAATGCA CCAACCTCTC ACGTTACGCC TTCCGTCAAG
    AAAAGAAGCA GCACCTTATC TCAACTCCCT GGGGATAAGT CCAGAGCCTT TGATTTCCTT
    AGCGAAGAAA CTGAAGCTAG TTTAGCCTCA CGGAGAGAAC AAAAGAGAGA ACAGTATCGT
    CAGGTAAAGG CCCATGTTCA GAAGGAAGAT GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG
    CCTCAGAAAT ACAAACAGGT AACCAACGGA CAAGGGGAAA ATAAGATGAA AAATTTACCT
    GTGCCTGTCT ATCTCAGACC TCTAGATGAA AAAGATGCAT CAATGAAGCT TTGGTGTGCT
    GTTGGAGTCA ATTTGTCTGG TGGGAAGACC AGAGATGGTG GTTCTGTTGT TGGAGCAAGT
    GTGTTTTACA AGGATGTTGC TGGTTTGGAA GCAGAAGGCA GTAAACAGCG AAGTGCATCT
    CAGAATAGCT TAGACAAGTT AGACCAGGAA CTTAAGGAAC AACAGAAGGA GTTAAAAAAT
    CAAGAAGAAT TATCCAGTCT AGTCTGGATC TGTACCAGCA CTCATTCAGC TACAAAAGTT
    ATCATTATCG ATGCTGTTCA ACCAGGCAAC ATCCTAGACA GCTTCACTGT TTGTAACTCT
    CACGTTCTGT GTATATCAAG CGTACCAGGA GCCCGAGAAA CAGACTACCC TGCAGGAGAA
    GAGCTTTCAG AATCTGGACA AGTAGACAAA GCATCTTTAT GTGGAAGCAT GACAAGCAAT
    AGCTCAGCAG AGACAGACAG CCTGTTGGGA GGCATCACAG TAGTTGGTTG TTCTGCAGAA
    GGTGTGACAG CAGCTGCCAC TTCCCCTAAT ACCAATGAAC CTTCTCCAGT GATGGAAAAA
    CCGCCAGAAA AGGAAACAGA AAATAGTGAG ATTGATGAAA ATGTTCCAAC AGCAGAAGAA
    GCAACTGAAG CTACAGAAGG CAATGCAGGA TCAGCTGAAG ATACTGTGGA TGTCTCCCAA
    ACTGGCGTGT ACACAGAGCA TGTGTTTACA GATCCTTTGG GAGTTCAAAT CCCAGAAGAC
    CTGTCACCAG TATATCAGTC AAGTAATGAC TCAGATGCAT ATAAAGATCA AATATCAGTA
    TTGCCAAATG AACAAGACCT GGTGAGAGAG GAAGCCCAGA AAATGAGTAG CCTTTTACCA
    ACTATGTGGC TTGGAGCTCA AAATGGGTGT TTGTATGTCC ATTCATCTGT AGCCCAGTGG
    AGGAAATGTC TCCATTCTAT TAAACTTAAA GATTCGATTC TCAGTATTGT ACACGTGAAA
    GGAATTGTGT TAGTGGCCCT AGCTGATGGG ACCCTTGCCA TCTTTCACAG AGGAGTTGAT
    GGACAGTGGG ATTTGTCAAA CTATCACCTG TTAGATCTTG GACGACCGCA CCATTCCATC
    CGATGCATGA CTGTGGTACA TGACAAAGTT TGGTGTGGCT ATAGGAACAA AATTTATGTG
    GTTCAGCCAA AGGCCATGAA AATAGAGAAA TCATTTGATG CACACCCCAG GAAGGAGAGC
    CAAGTGCGGC AGCTTGCCTG GGTGGGTGAT GGTGTGTGGG TCTCCATTCG CTTGGATTCC
    ACACTCCGTC TCTATCATGC ACACACTTAT CAGCATCTGC AGGATGTGGA CATTGAGCCT
    TATGTAAGCA AAATGCTAGG TACTGGAAAA CTGGGCTTCT CTTTTGTGAG GATCACAGCG
    CTTATGGTGT CTTGTAACCG TTTGTGGGTG GGAACAGGAA ATGGTGTCAT TATCTCCATC
    CCATTGACAG AAACGAATAA AACATCAGGA GCATCGGGAA ATCGTCCTGG AAGTGTCATT
    CGAGTATATG GTGATGAAAA CAGTGATAAA GTGACACCAG GGACATTTAT ACCCTATTGT
    TCAATGGCAC ATGCACAGCT TTGCTTCCAT GGGCACAGGG ATGCTGTGAA ATTTTTTGTG
    GCTGTCCCAG GTCAAGTCAT CAGCCCACAA AGTGGCAGCA GTGGTACAGA TCTGACAGTT
    GACAAAGCAG GGCCATCTGC ACAGGAGCCT GGCATCCAGA CACCCTTGAA GTCTGTGCTT
    GTCATTAGTG GAGGAGAGGG CTACATTGAC TTCCGAATGG GTGATGAAGG TGGAGAATCA
    GAGCTTCTTG GAGAGGATCT TCCACTTGAA CCTTCTGTCG CCAAAGCAGA AAGGAGTCAC
    TTGATAGTGT GGCAAGTGAT GTATGGTAGT GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29955
    Clone ID Related Accession (Same CDS sequence) XM_005220620.3
    Accession Version XM_005220620.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220620.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
    AATTCTGCTA TCTTAGTATC TCTCTCCGTC TTGCTGCTTG TGCACTTCTC TATTTCTACC
    GGAGTGCCAG CTTTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG TCCTATATCG
    TTAGGGATTT TCCCTTTACC TTCTGGAGAC GGATTGCTTA CGCCTGACAC TCAGAAAGGT
    GGCGAGACCC CTGGATCAGA ACAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT
    ACCAGTCTGA AGGATGAACT TTCCGATGTT AGCCAGGGAG GATCTAAAGC CACCACTCCA
    GCGTCGACAG CTGCTTCAGA TGTGGCAGCA ACACCTAGCG ATACTCCCTT ACATGAGGAG
    AACGGAGGGG TTGTGGAGGT TGCAGATACA CCCGATAAGT CAGAGATAAG CAAGCATATC
    GAAGTACACG TAGTCCAGGA AACTAGAAAC ATAGCAACGG GTTCTGCTGA AAATGAAGAG
    AAGTCAGAAG TTCAGGCAAT CATTGAATCC ACTCCTGAGT TGGATATGGA CAAAGATCTC
    AGTGGATATA AAGGCTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC
    AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGTT CAGGTCTAAT TGGAGATGTG
    GATGAAGGAG CAGATTTACT AGGAATGGGT CGTGAAGTTG AAAATCTCAT ATTGGAAAAT
    ACACAACTAT TGGAAACCAA AAATGCCTTG AATGTAGTGA AGAATGACTT GATAGCAAAA
    GTAGATGAAC TTACCTGTGA GAAAGATGTG CTGCAAGGGG AGCTGGAGGC TGTGAAACAA
    GCCAAGCTGA AGCTGGAGGA AAAGAACAAA GAATTGGAAG AAGAGCTTAG GAAAGCTCGT
    GCAGAAGTTG AAGATGCAAA GCAAAAAGCA AAAGATGATG ATGATAGTGA TATTCCCACA
    GCCCAAAGGA AGCGGTTTAC TAGAGTAGAA ATGGCCCGAG TTCTAATGGA GAGAAACCAA
    TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGTGCG
    TCAAGAGAAA ATCCAGCCAT GCAGGAGAAG AAAAGGTCAA GCATTTGGCA GTTTTTCAGC
    CGACTTTTCA GCTCCTCAAG TAACACTACT AAGAAGCCCG AACCACCAGT TAATCTGAAG
    TACAATGCAC CAACCTCTCA CGTTACGCCT TCCGTCAAGA AAAGAAGCAG CACCTTATCT
    CAACTCCCTG GGGATAAGTC CAGAGCCTTT GATTTCCTTA GCGAAGAAAC TGAAGCTAGT
    TTAGCCTCAC GGAGAGAACA AAAGAGAGAA CAGTATCGTC AGGTAAAGGC CCATGTTCAG
    AAGGAAGATG GTAGAGTGCA GGCTTTTGGC TGGAGTCTGC CTCAGAAATA CAAACAGGTA
    ACCAACGGAC AAGGGGAAAA TAAGATGAAA AATTTACCTG TGCCTGTCTA TCTCAGACCT
    CTAGATGAAA AAGATGCATC AATGAAGCTT TGGTGTGCTG TTGGAGTCAA TTTGTCTGGT
    GGGAAGACCA GAGATGGTGG TTCTGTTGTT GGAGCAAGTG TGTTTTACAA GGATGTTGCT
    GGTTTGGAAG CAGAAGGCAG TAAACAGCGA AGTGCATCTC AGAATAGCTT AGACAAGTTA
    GACCAGGAAC TTAAGGAACA ACAGAAGGAG TTAAAAAATC AAGAAGAATT ATCCAGTCTA
    GTCTGGATCT GTACCAGCAC TCATTCAGCT ACAAAAGTTA TCATTATCGA TGCTGTTCAA
    CCAGGCAACA TCCTAGACAG CTTCACTGTT TGTAACTCTC ACGTTCTGTG TATATCAAGC
    GTACCAGGAG CCCGAGAAAC AGACTACCCT GCAGGAGAAG AGCTTTCAGA ATCTGGACAA
    GTAGACAAAG CATCTTTATG TGGAAGCATG ACAAGCAATA GCTCAGCAGA GACAGACAGC
    CTGTTGGGAG GCATCACAGT AGTTGGTTGT TCTGCAGAAG GTGTGACAGC AGCTGCCACT
    TCCCCTAATA CCAATGAACC TTCTCCAGTG ATGGAAAAAC CGCCAGAAAA GGAAACAGAA
    AATAGTGAGA TTGATGAAAA TGTTCCAACA GCAGAAGAAG CAACTGAAGC TACAGAAGGC
    AATGCAGGAT CAGCTGAAGA TACTGTGGAT GTCTCCCAAA CTGGCGTGTA CACAGAGCAT
    GTGTTTACAG ATCCTTTGGG AGTTCAAATC CCAGAAGACC TGTCACCAGT ATATCAGTCA
    AGTAATGACT CAGATGCATA TAAAGATCAA ATATCAGTAT TGCCAAATGA ACAAGACCTG
    GTGAGAGAGG AAGCCCAGAA AATGAGTAGC CTTTTACCAA CTATGTGGCT TGGAGCTCAA
    AATGGGTGTT TGTATGTCCA TTCATCTGTA GCCCAGTGGA GGAAATGTCT CCATTCTATT
    AAACTTAAAG ATTCGATTCT CAGTATTGTA CACGTGAAAG GAATTGTGTT AGTGGCCCTA
    GCTGATGGGA CCCTTGCCAT CTTTCACAGA GGAGTTGATG GACAGTGGGA TTTGTCAAAC
    TATCACCTGT TAGATCTTGG ACGACCGCAC CATTCCATCC GATGCATGAC TGTGGTACAT
    GACAAAGTTT GGTGTGGCTA TAGGAACAAA ATTTATGTGG TTCAGCCAAA GGCCATGAAA
    ATAGAGAAAT CATTTGATGC ACACCCCAGG AAGGAGAGCC AAGTGCGGCA GCTTGCCTGG
    GTGGGTGATG GTGTGTGGGT CTCCATTCGC TTGGATTCCA CACTCCGTCT CTATCATGCA
    CACACTTATC AGCATCTGCA GGATGTGGAC ATTGAGCCTT ATGTAAGCAA AATGCTAGGT
    ACTGGAAAAC TGGGCTTCTC TTTTGTGAGG ATCACAGCGC TTATGGTGTC TTGTAACCGT
    TTGTGGGTGG GAACAGGAAA TGGTGTCATT ATCTCCATCC CATTGACAGA AACGAATAAA
    ACATCAGGAG CATCGGGAAA TCGTCCTGGA AGTGTCATTC GAGTATATGG TGATGAAAAC
    AGTGATAAAG TGACACCAGG GACATTTATA CCCTATTGTT CAATGGCACA TGCACAGCTT
    TGCTTCCATG GGCACAGGGA TGCTGTGAAA TTTTTTGTGG CTGTCCCAGG TCAAGTCATC
    AGCCCACAAA GTGGCAGCAG TGGTACAGAT CTGACAGTTG ACAAAGCAGG GCCATCTGCA
    CAGGAGCCTG GCATCCAGAC ACCCTTGAAG TCTGTGCTTG TCATTAGTGG AGGAGAGGGC
    TACATTGACT TCCGAATGGG TGATGAAGGT GGAGAATCAG AGCTTCTTGG AGAGGATCTT
    CCACTTGAAC CTTCTGTCGC CAAAGCAGAA AGGAGTCACT TGATAGTGTG GCAAGTGATG
    TATGGTAGTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220677.1
    CDS377..3871
    Translation

    Target ORF information:

    RefSeq Version XM_005220620.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220620.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
    AATTCTGCTA TCTTAGTATC TCTCTCCGTC TTGCTGCTTG TGCACTTCTC TATTTCTACC
    GGAGTGCCAG CTTTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG TCCTATATCG
    TTAGGGATTT TCCCTTTACC TTCTGGAGAC GGATTGCTTA CGCCTGACAC TCAGAAAGGT
    GGCGAGACCC CTGGATCAGA ACAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT
    ACCAGTCTGA AGGATGAACT TTCCGATGTT AGCCAGGGAG GATCTAAAGC CACCACTCCA
    GCGTCGACAG CTGCTTCAGA TGTGGCAGCA ACACCTAGCG ATACTCCCTT ACATGAGGAG
    AACGGAGGGG TTGTGGAGGT TGCAGATACA CCCGATAAGT CAGAGATAAG CAAGCATATC
    GAAGTACACG TAGTCCAGGA AACTAGAAAC ATAGCAACGG GTTCTGCTGA AAATGAAGAG
    AAGTCAGAAG TTCAGGCAAT CATTGAATCC ACTCCTGAGT TGGATATGGA CAAAGATCTC
    AGTGGATATA AAGGCTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC
    AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGTT CAGGTCTAAT TGGAGATGTG
    GATGAAGGAG CAGATTTACT AGGAATGGGT CGTGAAGTTG AAAATCTCAT ATTGGAAAAT
    ACACAACTAT TGGAAACCAA AAATGCCTTG AATGTAGTGA AGAATGACTT GATAGCAAAA
    GTAGATGAAC TTACCTGTGA GAAAGATGTG CTGCAAGGGG AGCTGGAGGC TGTGAAACAA
    GCCAAGCTGA AGCTGGAGGA AAAGAACAAA GAATTGGAAG AAGAGCTTAG GAAAGCTCGT
    GCAGAAGTTG AAGATGCAAA GCAAAAAGCA AAAGATGATG ATGATAGTGA TATTCCCACA
    GCCCAAAGGA AGCGGTTTAC TAGAGTAGAA ATGGCCCGAG TTCTAATGGA GAGAAACCAA
    TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGTGCG
    TCAAGAGAAA ATCCAGCCAT GCAGGAGAAG AAAAGGTCAA GCATTTGGCA GTTTTTCAGC
    CGACTTTTCA GCTCCTCAAG TAACACTACT AAGAAGCCCG AACCACCAGT TAATCTGAAG
    TACAATGCAC CAACCTCTCA CGTTACGCCT TCCGTCAAGA AAAGAAGCAG CACCTTATCT
    CAACTCCCTG GGGATAAGTC CAGAGCCTTT GATTTCCTTA GCGAAGAAAC TGAAGCTAGT
    TTAGCCTCAC GGAGAGAACA AAAGAGAGAA CAGTATCGTC AGGTAAAGGC CCATGTTCAG
    AAGGAAGATG GTAGAGTGCA GGCTTTTGGC TGGAGTCTGC CTCAGAAATA CAAACAGGTA
    ACCAACGGAC AAGGGGAAAA TAAGATGAAA AATTTACCTG TGCCTGTCTA TCTCAGACCT
    CTAGATGAAA AAGATGCATC AATGAAGCTT TGGTGTGCTG TTGGAGTCAA TTTGTCTGGT
    GGGAAGACCA GAGATGGTGG TTCTGTTGTT GGAGCAAGTG TGTTTTACAA GGATGTTGCT
    GGTTTGGAAG CAGAAGGCAG TAAACAGCGA AGTGCATCTC AGAATAGCTT AGACAAGTTA
    GACCAGGAAC TTAAGGAACA ACAGAAGGAG TTAAAAAATC AAGAAGAATT ATCCAGTCTA
    GTCTGGATCT GTACCAGCAC TCATTCAGCT ACAAAAGTTA TCATTATCGA TGCTGTTCAA
    CCAGGCAACA TCCTAGACAG CTTCACTGTT TGTAACTCTC ACGTTCTGTG TATATCAAGC
    GTACCAGGAG CCCGAGAAAC AGACTACCCT GCAGGAGAAG AGCTTTCAGA ATCTGGACAA
    GTAGACAAAG CATCTTTATG TGGAAGCATG ACAAGCAATA GCTCAGCAGA GACAGACAGC
    CTGTTGGGAG GCATCACAGT AGTTGGTTGT TCTGCAGAAG GTGTGACAGC AGCTGCCACT
    TCCCCTAATA CCAATGAACC TTCTCCAGTG ATGGAAAAAC CGCCAGAAAA GGAAACAGAA
    AATAGTGAGA TTGATGAAAA TGTTCCAACA GCAGAAGAAG CAACTGAAGC TACAGAAGGC
    AATGCAGGAT CAGCTGAAGA TACTGTGGAT GTCTCCCAAA CTGGCGTGTA CACAGAGCAT
    GTGTTTACAG ATCCTTTGGG AGTTCAAATC CCAGAAGACC TGTCACCAGT ATATCAGTCA
    AGTAATGACT CAGATGCATA TAAAGATCAA ATATCAGTAT TGCCAAATGA ACAAGACCTG
    GTGAGAGAGG AAGCCCAGAA AATGAGTAGC CTTTTACCAA CTATGTGGCT TGGAGCTCAA
    AATGGGTGTT TGTATGTCCA TTCATCTGTA GCCCAGTGGA GGAAATGTCT CCATTCTATT
    AAACTTAAAG ATTCGATTCT CAGTATTGTA CACGTGAAAG GAATTGTGTT AGTGGCCCTA
    GCTGATGGGA CCCTTGCCAT CTTTCACAGA GGAGTTGATG GACAGTGGGA TTTGTCAAAC
    TATCACCTGT TAGATCTTGG ACGACCGCAC CATTCCATCC GATGCATGAC TGTGGTACAT
    GACAAAGTTT GGTGTGGCTA TAGGAACAAA ATTTATGTGG TTCAGCCAAA GGCCATGAAA
    ATAGAGAAAT CATTTGATGC ACACCCCAGG AAGGAGAGCC AAGTGCGGCA GCTTGCCTGG
    GTGGGTGATG GTGTGTGGGT CTCCATTCGC TTGGATTCCA CACTCCGTCT CTATCATGCA
    CACACTTATC AGCATCTGCA GGATGTGGAC ATTGAGCCTT ATGTAAGCAA AATGCTAGGT
    ACTGGAAAAC TGGGCTTCTC TTTTGTGAGG ATCACAGCGC TTATGGTGTC TTGTAACCGT
    TTGTGGGTGG GAACAGGAAA TGGTGTCATT ATCTCCATCC CATTGACAGA AACGAATAAA
    ACATCAGGAG CATCGGGAAA TCGTCCTGGA AGTGTCATTC GAGTATATGG TGATGAAAAC
    AGTGATAAAG TGACACCAGG GACATTTATA CCCTATTGTT CAATGGCACA TGCACAGCTT
    TGCTTCCATG GGCACAGGGA TGCTGTGAAA TTTTTTGTGG CTGTCCCAGG TCAAGTCATC
    AGCCCACAAA GTGGCAGCAG TGGTACAGAT CTGACAGTTG ACAAAGCAGG GCCATCTGCA
    CAGGAGCCTG GCATCCAGAC ACCCTTGAAG TCTGTGCTTG TCATTAGTGG AGGAGAGGGC
    TACATTGACT TCCGAATGGG TGATGAAGGT GGAGAATCAG AGCTTCTTGG AGAGGATCTT
    CCACTTGAAC CTTCTGTCGC CAAAGCAGAA AGGAGTCACT TGATAGTGTG GCAAGTGATG
    TATGGTAGTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54790
    Clone ID Related Accession (Same CDS sequence) XM_010816157.2
    Accession Version XM_010816157.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816157.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTATGCCAG CTCGTTTCAG CCGACTTTTC AGCTCCTCAA GTAACACTAC TAAGAAGCCC
    GAACCACCAG TTAATCTGAA GTACAATGCA CCAACCTCTC ACGTTACGCC TTCCGTCAAG
    AAAAGAAGCA GCACCTTATC TCAACTCCCT GGGGATAAGT CCAGAGCCTT TGATTTCCTT
    AGCGAAGAAA CTGAAGCTAG TTTAGCCTCA CGGAGAGAAC AAAAGAGAGA ACAGTATCGT
    CAGGTAAAGG CCCATGTTCA GAAGGAAGAT GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG
    CCTCAGAAAT ACAAACAGGT AACCAACGGA CAAGGGGAAA ATAAGATGAA AAATTTACCT
    GTGCCTGTCT ATCTCAGACC TCTAGATGAA AAAGATGCAT CAATGAAGCT TTGGTGTGCT
    GTTGGAGTCA ATTTGTCTGG TGGGAAGACC AGAGATGGTG GTTCTGTTGT TGGAGCAAGT
    GTGTTTTACA AGGATGTTGC TGGTTTGGAA GCAGAAGGCA GTAAACAGCG AAGTGCATCT
    CAGAATAGCT TAGACAAGTT AGACCAGGAA CTTAAGGAAC AACAGAAGGA GTTAAAAAAT
    CAAGAAGAAT TATCCAGTCT AGTCTGGATC TGTACCAGCA CTCATTCAGC TACAAAAGTT
    ATCATTATCG ATGCTGTTCA ACCAGGCAAC ATCCTAGACA GCTTCACTGT TTGTAACTCT
    CACGTTCTGT GTATATCAAG CGTACCAGGA GCCCGAGAAA CAGACTACCC TGCAGGAGAA
    GAGCTTTCAG AATCTGGACA AGTAGACAAA GCATCTTTAT GTGGAAGCAT GACAAGCAAT
    AGCTCAGCAG AGACAGACAG CCTGTTGGGA GGCATCACAG TAGTTGGTTG TTCTGCAGAA
    GGTGTGACAG CAGCTGCCAC TTCCCCTAAT ACCAATGAAC CTTCTCCAGT GATGGAAAAA
    CCGCCAGAAA AGGAAACAGA AAATAGTGAG ATTGATGAAA ATGTTCCAAC AGCAGAAGAA
    GCAACTGAAG CTACAGAAGG CAATGCAGGA TCAGCTGAAG ATACTGTGGA TGTCTCCCAA
    ACTGGCGTGT ACACAGAGCA TGTGTTTACA GATCCTTTGG GAGTTCAAAT CCCAGAAGAC
    CTGTCACCAG TATATCAGTC AAGTAATGAC TCAGATGCAT ATAAAGATCA AATATCAGTA
    TTGCCAAATG AACAAGACCT GGTGAGAGAG GAAGCCCAGA AAATGAGTAG CCTTTTACCA
    ACTATGTGGC TTGGAGCTCA AAATGGGTGT TTGTATGTCC ATTCATCTGT AGCCCAGTGG
    AGGAAATGTC TCCATTCTAT TAAACTTAAA GATTCGATTC TCAGTATTGT ACACGTGAAA
    GGAATTGTGT TAGTGGCCCT AGCTGATGGG ACCCTTGCCA TCTTTCACAG AGGAGTTGAT
    GGACAGTGGG ATTTGTCAAA CTATCACCTG TTAGATCTTG GACGACCGCA CCATTCCATC
    CGATGCATGA CTGTGGTACA TGACAAAGTT TGGTGTGGCT ATAGGAACAA AATTTATGTG
    GTTCAGCCAA AGGCCATGAA AATAGAGAAA TCATTTGATG CACACCCCAG GAAGGAGAGC
    CAAGTGCGGC AGCTTGCCTG GGTGGGTGAT GGTGTGTGGG TCTCCATTCG CTTGGATTCC
    ACACTCCGTC TCTATCATGC ACACACTTAT CAGCATCTGC AGGATGTGGA CATTGAGCCT
    TATGTAAGCA AAATGCTAGG TACTGGAAAA CTGGGCTTCT CTTTTGTGAG GATCACAGCG
    CTTATGGTGT CTTGTAACCG TTTGTGGGTG GGAACAGGAA ATGGTGTCAT TATCTCCATC
    CCATTGACAG AAACCGTAAT CCTCCACCAG GGACGTTTAC TGGGGCTGAG GGCGAATAAA
    ACATCAGGAG CATCGGGAAA TCGTCCTGGA AGTGTCATTC GAGTATATGG TGATGAAAAC
    AGTGATAAAG TGACACCAGG GACATTTATA CCCTATTGTT CAATGGCACA TGCACAGCTT
    TGCTTCCATG GGCACAGGGA TGCTGTGAAA TTTTTTGTGG CTGTCCCAGG TCAAGTCATC
    AGCCCACAAA GTGGCAGCAG TGGTACAGAT CTGACAGTTG ACAAAGCAGG GCCATCTGCA
    CAGGAGCCTG GCATCCAGAC ACCCTTGAAG TCTGTGCTTG TCATTAGTGG AGGAGAGGGC
    TACATTGACT TCCGAATGGG TGATGAAGGT GGAGAATCAG AGCTTCTTGG AGAGGATCTT
    CCACTTGAAC CTTCTGTCGC CAAAGCAGAA AGGAGTCACT TGATAGTGTG GCAAGTGATG
    TATGGTAGTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814459.1
    CDS117..4091
    Translation

    Target ORF information:

    RefSeq Version XM_010816157.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816157.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTATGCCAG CTCGTTTCAG CCGACTTTTC AGCTCCTCAA GTAACACTAC TAAGAAGCCC
    GAACCACCAG TTAATCTGAA GTACAATGCA CCAACCTCTC ACGTTACGCC TTCCGTCAAG
    AAAAGAAGCA GCACCTTATC TCAACTCCCT GGGGATAAGT CCAGAGCCTT TGATTTCCTT
    AGCGAAGAAA CTGAAGCTAG TTTAGCCTCA CGGAGAGAAC AAAAGAGAGA ACAGTATCGT
    CAGGTAAAGG CCCATGTTCA GAAGGAAGAT GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG
    CCTCAGAAAT ACAAACAGGT AACCAACGGA CAAGGGGAAA ATAAGATGAA AAATTTACCT
    GTGCCTGTCT ATCTCAGACC TCTAGATGAA AAAGATGCAT CAATGAAGCT TTGGTGTGCT
    GTTGGAGTCA ATTTGTCTGG TGGGAAGACC AGAGATGGTG GTTCTGTTGT TGGAGCAAGT
    GTGTTTTACA AGGATGTTGC TGGTTTGGAA GCAGAAGGCA GTAAACAGCG AAGTGCATCT
    CAGAATAGCT TAGACAAGTT AGACCAGGAA CTTAAGGAAC AACAGAAGGA GTTAAAAAAT
    CAAGAAGAAT TATCCAGTCT AGTCTGGATC TGTACCAGCA CTCATTCAGC TACAAAAGTT
    ATCATTATCG ATGCTGTTCA ACCAGGCAAC ATCCTAGACA GCTTCACTGT TTGTAACTCT
    CACGTTCTGT GTATATCAAG CGTACCAGGA GCCCGAGAAA CAGACTACCC TGCAGGAGAA
    GAGCTTTCAG AATCTGGACA AGTAGACAAA GCATCTTTAT GTGGAAGCAT GACAAGCAAT
    AGCTCAGCAG AGACAGACAG CCTGTTGGGA GGCATCACAG TAGTTGGTTG TTCTGCAGAA
    GGTGTGACAG CAGCTGCCAC TTCCCCTAAT ACCAATGAAC CTTCTCCAGT GATGGAAAAA
    CCGCCAGAAA AGGAAACAGA AAATAGTGAG ATTGATGAAA ATGTTCCAAC AGCAGAAGAA
    GCAACTGAAG CTACAGAAGG CAATGCAGGA TCAGCTGAAG ATACTGTGGA TGTCTCCCAA
    ACTGGCGTGT ACACAGAGCA TGTGTTTACA GATCCTTTGG GAGTTCAAAT CCCAGAAGAC
    CTGTCACCAG TATATCAGTC AAGTAATGAC TCAGATGCAT ATAAAGATCA AATATCAGTA
    TTGCCAAATG AACAAGACCT GGTGAGAGAG GAAGCCCAGA AAATGAGTAG CCTTTTACCA
    ACTATGTGGC TTGGAGCTCA AAATGGGTGT TTGTATGTCC ATTCATCTGT AGCCCAGTGG
    AGGAAATGTC TCCATTCTAT TAAACTTAAA GATTCGATTC TCAGTATTGT ACACGTGAAA
    GGAATTGTGT TAGTGGCCCT AGCTGATGGG ACCCTTGCCA TCTTTCACAG AGGAGTTGAT
    GGACAGTGGG ATTTGTCAAA CTATCACCTG TTAGATCTTG GACGACCGCA CCATTCCATC
    CGATGCATGA CTGTGGTACA TGACAAAGTT TGGTGTGGCT ATAGGAACAA AATTTATGTG
    GTTCAGCCAA AGGCCATGAA AATAGAGAAA TCATTTGATG CACACCCCAG GAAGGAGAGC
    CAAGTGCGGC AGCTTGCCTG GGTGGGTGAT GGTGTGTGGG TCTCCATTCG CTTGGATTCC
    ACACTCCGTC TCTATCATGC ACACACTTAT CAGCATCTGC AGGATGTGGA CATTGAGCCT
    TATGTAAGCA AAATGCTAGG TACTGGAAAA CTGGGCTTCT CTTTTGTGAG GATCACAGCG
    CTTATGGTGT CTTGTAACCG TTTGTGGGTG GGAACAGGAA ATGGTGTCAT TATCTCCATC
    CCATTGACAG AAACCGTAAT CCTCCACCAG GGACGTTTAC TGGGGCTGAG GGCGAATAAA
    ACATCAGGAG CATCGGGAAA TCGTCCTGGA AGTGTCATTC GAGTATATGG TGATGAAAAC
    AGTGATAAAG TGACACCAGG GACATTTATA CCCTATTGTT CAATGGCACA TGCACAGCTT
    TGCTTCCATG GGCACAGGGA TGCTGTGAAA TTTTTTGTGG CTGTCCCAGG TCAAGTCATC
    AGCCCACAAA GTGGCAGCAG TGGTACAGAT CTGACAGTTG ACAAAGCAGG GCCATCTGCA
    CAGGAGCCTG GCATCCAGAC ACCCTTGAAG TCTGTGCTTG TCATTAGTGG AGGAGAGGGC
    TACATTGACT TCCGAATGGG TGATGAAGGT GGAGAATCAG AGCTTCTTGG AGAGGATCTT
    CCACTTGAAC CTTCTGTCGC CAAAGCAGAA AGGAGTCACT TGATAGTGTG GCAAGTGATG
    TATGGTAGTG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54791
    Clone ID Related Accession (Same CDS sequence) XM_010816158.2
    Accession Version XM_010816158.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816158.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTATGCCAG CTCGTTTCAG CCGACTTTTC AGCTCCTCAA GTAACACTAC TAAGAAGCCC
    GAACCACCAG TTAATCTGAA GTACAATGCA CCAACCTCTC ACGTTACGCC TTCCGTCAAG
    AAAAGAAGCA GCACCTTATC TCAACTCCCT GGGGATAAGT CCAGAGCCTT TGATTTCCTT
    AGCGAAGAAA CTGAAGCTAG TTTAGCCTCA CGGAGAGAAC AAAAGAGAGA ACAGTATCGT
    CAGGTAAAGG CCCATGTTCA GAAGGAAGAT GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG
    CCTCAGAAAT ACAAACAGGT AACCAACGGA CAAGGGGAAA ATAAGATGAA AAATTTACCT
    GTGCCTGTCT ATCTCAGACC TCTAGATGAA AAAGATGCAT CAATGAAGCT TTGGTGTGCT
    GTTGGAGTCA ATTTGTCTGG TGGGAAGACC AGAGATGGTG GTTCTGTTGT TGGAGCAAGT
    GTGTTTTACA AGGATGTTGC TGGTTTGGAA GCAGAAGGCA GTAAACAGCG AAGTGCATCT
    CAGAATAGCT TAGACAAGTT AGACCAGGAA CTTAAGGAAC AACAGAAGGA GTTAAAAAAT
    CAAGAAGAAT TATCCAGTCT AGTCTGGATC TGTACCAGCA CTCATTCAGC TACAAAAGTT
    ATCATTATCG ATGCTGTTCA ACCAGGCAAC ATCCTAGACA GCTTCACTGT TTGTAACTCT
    CACGTTCTGT GTATATCAAG CGTACCAGGA GCCCGAGAAA CAGACTACCC TGCAGGAGAA
    GAGCTTTCAG AATCTGGACA AGTAGACAAA GCATCTTTAT GTGGAAGCAT GACAAGCAAT
    AGCTCAGCAG AGACAGACAG CCTGTTGGGA GGCATCACAG TAGTTGGTTG TTCTGCAGAA
    GGTGTGACAG CAGCTGCCAC TTCCCCTAAT ACCAATGAAC CTTCTCCAGT GATGGAAAAA
    CCGCCAGCAG AAGAAGCAAC TGAAGCTACA GAAGGCAATG CAGGATCAGC TGAAGATACT
    GTGGATGTCT CCCAAACTGG CGTGTACACA GAGCATGTGT TTACAGATCC TTTGGGAGTT
    CAAATCCCAG AAGACCTGTC ACCAGTATAT CAGTCAAGTA ATGACTCAGA TGCATATAAA
    GATCAAATAT CAGTATTGCC AAATGAACAA GACCTGGTGA GAGAGGAAGC CCAGAAAATG
    AGTAGCCTTT TACCAACTAT GTGGCTTGGA GCTCAAAATG GGTGTTTGTA TGTCCATTCA
    TCTGTAGCCC AGTGGAGGAA ATGTCTCCAT TCTATTAAAC TTAAAGATTC GATTCTCAGT
    ATTGTACACG TGAAAGGAAT TGTGTTAGTG GCCCTAGCTG ATGGGACCCT TGCCATCTTT
    CACAGAGGAG TTGATGGACA GTGGGATTTG TCAAACTATC ACCTGTTAGA TCTTGGACGA
    CCGCACCATT CCATCCGATG CATGACTGTG GTACATGACA AAGTTTGGTG TGGCTATAGG
    AACAAAATTT ATGTGGTTCA GCCAAAGGCC ATGAAAATAG AGAAATCATT TGATGCACAC
    CCCAGGAAGG AGAGCCAAGT GCGGCAGCTT GCCTGGGTGG GTGATGGTGT GTGGGTCTCC
    ATTCGCTTGG ATTCCACACT CCGTCTCTAT CATGCACACA CTTATCAGCA TCTGCAGGAT
    GTGGACATTG AGCCTTATGT AAGCAAAATG CTAGGTACTG GAAAACTGGG CTTCTCTTTT
    GTGAGGATCA CAGCGCTTAT GGTGTCTTGT AACCGTTTGT GGGTGGGAAC AGGAAATGGT
    GTCATTATCT CCATCCCATT GACAGAAACC GTAATCCTCC ACCAGGGACG TTTACTGGGG
    CTGAGGGCGA ATAAAACATC AGGAGCATCG GGAAATCGTC CTGGAAGTGT CATTCGAGTA
    TATGGTGATG AAAACAGTGA TAAAGTGACA CCAGGGACAT TTATACCCTA TTGTTCAATG
    GCACATGCAC AGCTTTGCTT CCATGGGCAC AGGGATGCTG TGAAATTTTT TGTGGCTGTC
    CCAGGTCAAG TCATCAGCCC ACAAAGTGGC AGCAGTGGTA CAGATCTGAC AGTTGACAAA
    GCAGGGCCAT CTGCACAGGA GCCTGGCATC CAGACACCCT TGAAGTCTGT GCTTGTCATT
    AGTGGAGGAG AGGGCTACAT TGACTTCCGA ATGGGTGATG AAGGTGGAGA ATCAGAGCTT
    CTTGGAGAGG ATCTTCCACT TGAACCTTCT GTCGCCAAAG CAGAAAGGAG TCACTTGATA
    GTGTGGCAAG TGATGTATGG TAGTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814460.1
    CDS118..4047
    Translation

    Target ORF information:

    RefSeq Version XM_010816158.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816158.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
    TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTCATCGGG
    CGCTACGACG AGGAGGTGGT CAAGGAGCTG ATGCCACTGG TGGTAGCCGT GCTGGAGAAC
    CTGGACTCGG TGTTCGCGCA GGACCAAGAG CACCAGGTGG AGCTGGAGCT GCTCCGGGAC
    GACAACGAGC AGCTCATCAC CCAGTACGAG CGAGAGAAGG CGCTGCGCAA GCACGCAGAG
    GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAAGAAA AAAAAGATTT ACAGACCAGA
    GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAACTCA AGGCCAAAAA CTATGCTGAC
    CAGATTAGCA GACTTGAAGA AAGAGAAGCG GAACTGAAGA AAGAATATAA TGCTTTGCAT
    CAGAGACACA CTGAGATGAT CCATAATTAT ATGGAACACT TAGAAAGAAC AAAACTTCAT
    CAGATCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGT
    CCTATATCGT TAGGGATTTT CCCTTTACCT TCTGGAGACG GATTGCTTAC GCCTGACACT
    CAGAAAGGTG GCGAGACCCC TGGATCAGAA CAATGGAAAT TTCAGGAATT AAGTCAACCA
    CGTTCTCATA CCAGTCTGAA GGATGAACTT TCCGATGTTA GCCAGGGAGG ATCTAAAGCC
    ACCACTCCAG CGTCGACAGC TGCTTCAGAT GTGGCAGCAA CACCTAGCGA TACTCCCTTA
    CATGAGGAGA ACGGAGGGGT TGTGGAGGTT GCAGATACAC CCGATAAGTC AGAGATAAGC
    AAGCATATCG AAGTACACGT AGTCCAGGAA ACTAGAAACA TAGCAACGGG TTCTGCTGAA
    AATGAAGAGA AGTCAGAAGT TCAGGCAATC ATTGAATCCA CTCCTGAGTT GGATATGGAC
    AAAGATCTCA GTGGATATAA AGGCTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT
    TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGTTC AGGTCTAATT
    GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GTGAAGTTGA AAATCTCATA
    TTGGAAAATA CACAACTATT GGAAACCAAA AATGCCTTGA ATGTAGTGAA GAATGACTTG
    ATAGCAAAAG TAGATGAACT TACCTGTGAG AAAGATGTGC TGCAAGGGGA GCTGGAGGCT
    GTGAAACAAG CCAAGCTGAA GCTGGAGGAA AAGAACAAAG AATTGGAAGA AGAGCTTAGG
    AAAGCTCGTG CAGAAGTTGA AGATGCAAAG CAAAAAGCAA AAGATGATGA TGATAGTGAT
    ATTCCCACAG CCCAAAGGAA GCGGTTTACT AGAGTAGAAA TGGCCCGAGT TCTAATGGAG
    AGAAACCAAT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG
    ATTCGTGCGT CAAGAGAAAA TCCAGCCATG CAGGAGAAGA AAAGGTCAAG CATTTGGCAG
    TTTATGCCAG CTCGTTTCAG CCGACTTTTC AGCTCCTCAA GTAACACTAC TAAGAAGCCC
    GAACCACCAG TTAATCTGAA GTACAATGCA CCAACCTCTC ACGTTACGCC TTCCGTCAAG
    AAAAGAAGCA GCACCTTATC TCAACTCCCT GGGGATAAGT CCAGAGCCTT TGATTTCCTT
    AGCGAAGAAA CTGAAGCTAG TTTAGCCTCA CGGAGAGAAC AAAAGAGAGA ACAGTATCGT
    CAGGTAAAGG CCCATGTTCA GAAGGAAGAT GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG
    CCTCAGAAAT ACAAACAGGT AACCAACGGA CAAGGGGAAA ATAAGATGAA AAATTTACCT
    GTGCCTGTCT ATCTCAGACC TCTAGATGAA AAAGATGCAT CAATGAAGCT TTGGTGTGCT
    GTTGGAGTCA ATTTGTCTGG TGGGAAGACC AGAGATGGTG GTTCTGTTGT TGGAGCAAGT
    GTGTTTTACA AGGATGTTGC TGGTTTGGAA GCAGAAGGCA GTAAACAGCG AAGTGCATCT
    CAGAATAGCT TAGACAAGTT AGACCAGGAA CTTAAGGAAC AACAGAAGGA GTTAAAAAAT
    CAAGAAGAAT TATCCAGTCT AGTCTGGATC TGTACCAGCA CTCATTCAGC TACAAAAGTT
    ATCATTATCG ATGCTGTTCA ACCAGGCAAC ATCCTAGACA GCTTCACTGT TTGTAACTCT
    CACGTTCTGT GTATATCAAG CGTACCAGGA GCCCGAGAAA CAGACTACCC TGCAGGAGAA
    GAGCTTTCAG AATCTGGACA AGTAGACAAA GCATCTTTAT GTGGAAGCAT GACAAGCAAT
    AGCTCAGCAG AGACAGACAG CCTGTTGGGA GGCATCACAG TAGTTGGTTG TTCTGCAGAA
    GGTGTGACAG CAGCTGCCAC TTCCCCTAAT ACCAATGAAC CTTCTCCAGT GATGGAAAAA
    CCGCCAGCAG AAGAAGCAAC TGAAGCTACA GAAGGCAATG CAGGATCAGC TGAAGATACT
    GTGGATGTCT CCCAAACTGG CGTGTACACA GAGCATGTGT TTACAGATCC TTTGGGAGTT
    CAAATCCCAG AAGACCTGTC ACCAGTATAT CAGTCAAGTA ATGACTCAGA TGCATATAAA
    GATCAAATAT CAGTATTGCC AAATGAACAA GACCTGGTGA GAGAGGAAGC CCAGAAAATG
    AGTAGCCTTT TACCAACTAT GTGGCTTGGA GCTCAAAATG GGTGTTTGTA TGTCCATTCA
    TCTGTAGCCC AGTGGAGGAA ATGTCTCCAT TCTATTAAAC TTAAAGATTC GATTCTCAGT
    ATTGTACACG TGAAAGGAAT TGTGTTAGTG GCCCTAGCTG ATGGGACCCT TGCCATCTTT
    CACAGAGGAG TTGATGGACA GTGGGATTTG TCAAACTATC ACCTGTTAGA TCTTGGACGA
    CCGCACCATT CCATCCGATG CATGACTGTG GTACATGACA AAGTTTGGTG TGGCTATAGG
    AACAAAATTT ATGTGGTTCA GCCAAAGGCC ATGAAAATAG AGAAATCATT TGATGCACAC
    CCCAGGAAGG AGAGCCAAGT GCGGCAGCTT GCCTGGGTGG GTGATGGTGT GTGGGTCTCC
    ATTCGCTTGG ATTCCACACT CCGTCTCTAT CATGCACACA CTTATCAGCA TCTGCAGGAT
    GTGGACATTG AGCCTTATGT AAGCAAAATG CTAGGTACTG GAAAACTGGG CTTCTCTTTT
    GTGAGGATCA CAGCGCTTAT GGTGTCTTGT AACCGTTTGT GGGTGGGAAC AGGAAATGGT
    GTCATTATCT CCATCCCATT GACAGAAACC GTAATCCTCC ACCAGGGACG TTTACTGGGG
    CTGAGGGCGA ATAAAACATC AGGAGCATCG GGAAATCGTC CTGGAAGTGT CATTCGAGTA
    TATGGTGATG AAAACAGTGA TAAAGTGACA CCAGGGACAT TTATACCCTA TTGTTCAATG
    GCACATGCAC AGCTTTGCTT CCATGGGCAC AGGGATGCTG TGAAATTTTT TGTGGCTGTC
    CCAGGTCAAG TCATCAGCCC ACAAAGTGGC AGCAGTGGTA CAGATCTGAC AGTTGACAAA
    GCAGGGCCAT CTGCACAGGA GCCTGGCATC CAGACACCCT TGAAGTCTGT GCTTGTCATT
    AGTGGAGGAG AGGGCTACAT TGACTTCCGA ATGGGTGATG AAGGTGGAGA ATCAGAGCTT
    CTTGGAGAGG ATCTTCCACT TGAACCTTCT GTCGCCAAAG CAGAAAGGAG TCACTTGATA
    GTGTGGCAAG TGATGTATGG TAGTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54792
    Clone ID Related Accession (Same CDS sequence) XM_010816159.2
    Accession Version XM_010816159.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product C-Jun-amino-terminal kinase-interacting protein 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104495.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816159.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
    AATTCTGCTA TCTTAGTATC TCTCTCCGTC TTGCTGCTTG TGCACTTCTC TATTTCTACC
    GGAGTGCCAG CTTTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG TCCTATATCG
    TTAGGGATTT TCCCTTTACC TTCTGGAGAC GGATTGCTTA CGCCTGACAC TCAGAAAGGT
    GGCGAGACCC CTGGATCAGA ACAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT
    ACCAGTCTGA AGGATGAACT TTCCGATGTT AGCCAGGGAG GATCTAAAGC CACCACTCCA
    GCGTCGACAG CTGCTTCAGA TGTGGCAGCA ACACCTAGCG ATACTCCCTT ACATGAGGAG
    AACGGAGGGG TTGTGGAGGT TGCAGATACA CCCGATAAGT CAGAGATAAG CAAGCATATC
    GAAGTACACG TAGTCCAGGA AACTAGAAAC ATAGCAACGG GTTCTGCTGA AAATGAAGAG
    AAGTCAGAAG TTCAGGCAAT CATTGAATCC ACTCCTGAGT TGGATATGGA CAAAGATCTC
    AGTGGATATA AAGGCTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC
    AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGTT CAGGTCTAAT TGGAGATGTG
    GATGAAGGAG CAGATTTACT AGGAATGGGT CGTGAAGTTG AAAATCTCAT ATTGGAAAAT
    ACACAACTAT TGGAAACCAA AAATGCCTTG AATGTAGTGA AGAATGACTT GATAGCAAAA
    GTAGATGAAC TTACCTGTGA GAAAGATGTG CTGCAAGGGG AGCTGGAGGC TGTGAAACAA
    GCCAAGCTGA AGCTGGAGGA AAAGAACAAA GAATTGGAAG AAGAGCTTAG GAAAGCTCGT
    GCAGAAGTTG AAGATGCAAA GCAAAAAGCA AAAGATGATG ATGATAGTGA TATTCCCACA
    GCCCAAAGGA AGCGGTTTAC TAGAGTAGAA ATGGCCCGAG TTCTAATGGA GAGAAACCAA
    TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGTGCG
    TCAAGAGAAA ATCCAGCCAT GCAGGAGAAG AAAAGGTCAA GCATTTGGCA GTTTATGCCA
    GCTCGTTTCA GCCGACTTTT CAGCTCCTCA AGTAACACTA CTAAGAAGCC CGAACCACCA
    GTTAATCTGA AGTACAATGC ACCAACCTCT CACGTTACGC CTTCCGTCAA GAAAAGAAGC
    AGCACCTTAT CTCAACTCCC TGGGGATAAG TCCAGAGCCT TTGATTTCCT TAGCGAAGAA
    ACTGAAGCTA GTTTAGCCTC ACGGAGAGAA CAAAAGAGAG AACAGTATCG TCAGGTAAAG
    GCCCATGTTC AGAAGGAAGA TGGTAGAGTG CAGGCTTTTG GCTGGAGTCT GCCTCAGAAA
    TACAAACAGG TAACCAACGG ACAAGGGGAA AATAAGATGA AAAATTTACC TGTGCCTGTC
    TATCTCAGAC CTCTAGATGA AAAAGATGCA TCAATGAAGC TTTGGTGTGC TGTTGGAGTC
    AATTTGTCTG GTGGGAAGAC CAGAGATGGT GGTTCTGTTG TTGGAGCAAG TGTGTTTTAC
    AAGGATGTTG CTGGTTTGGA AGCAGAAGGC AGTAAACAGC GAAGTGCATC TCAGAATAGC
    TTAGACAAGT TAGACCAGGA ACTTAAGGAA CAACAGAAGG AGTTAAAAAA TCAAGAAGAA
    TTATCCAGTC TAGTCTGGAT CTGTACCAGC ACTCATTCAG CTACAAAAGT TATCATTATC
    GATGCTGTTC AACCAGGCAA CATCCTAGAC AGCTTCACTG TTTGTAACTC TCACGTTCTG
    TGTATATCAA GCGTACCAGG AGCCCGAGAA ACAGACTACC CTGCAGGAGA AGAGCTTTCA
    GAATCTGGAC AAGTAGACAA AGCATCTTTA TGTGGAAGCA TGACAAGCAA TAGCTCAGCA
    GAGACAGACA GCCTGTTGGG AGGCATCACA GTAGTTGGTT GTTCTGCAGA AGGTGTGACA
    GCAGCTGCCA CTTCCCCTAA TACCAATGAA CCTTCTCCAG TGATGGAAAA ACCGCCAGAA
    AAGGAAACAG AAAATAGTGA GATTGATGAA AATGTTCCAA CAGCAGAAGA AGCAACTGAA
    GCTACAGAAG GCAATGCAGG ATCAGCTGAA GATACTGTGG ATGTCTCCCA AACTGGCGTG
    TACACAGAGC ATGTGTTTAC AGATCCTTTG GGAGTTCAAA TCCCAGAAGA CCTGTCACCA
    GTATATCAGT CAAGTAATGA CTCAGATGCA TATAAAGATC AAATATCAGT ATTGCCAAAT
    GAACAAGACC TGGTGAGAGA GGAAGCCCAG AAAATGAGTA GCCTTTTACC AACTATGTGG
    CTTGGAGCTC AAAATGGGTG TTTGTATGTC CATTCATCTG TAGCCCAGTG GAGGAAATGT
    CTCCATTCTA TTAAACTTAA AGATTCGATT CTCAGTATTG TACACGTGAA AGGAATTGTG
    TTAGTGGCCC TAGCTGATGG GACCCTTGCC ATCTTTCACA GAGGAGTTGA TGGACAGTGG
    GATTTGTCAA ACTATCACCT GTTAGATCTT GGACGACCGC ACCATTCCAT CCGATGCATG
    ACTGTGGTAC ATGACAAAGT TTGGTGTGGC TATAGGAACA AAATTTATGT GGTTCAGCCA
    AAGGCCATGA AAATAGAGAA ATCATTTGAT GCACACCCCA GGAAGGAGAG CCAAGTGCGG
    CAGCTTGCCT GGGTGGGTGA TGGTGTGTGG GTCTCCATTC GCTTGGATTC CACACTCCGT
    CTCTATCATG CACACACTTA TCAGCATCTG CAGGATGTGG ACATTGAGCC TTATGTAAGC
    AAAATGCTAG GTACTGGAAA ACTGGGCTTC TCTTTTGTGA GGATCACAGC GCTTATGGTG
    TCTTGTAACC GTTTGTGGGT GGGAACAGGA AATGGTGTCA TTATCTCCAT CCCATTGACA
    GAAACCGTAA TCCTCCACCA GGGACGTTTA CTGGGGCTGA GGGCGAATAA AACATCAGGA
    GCATCGGGAA ATCGTCCTGG AAGTGTCATT CGAGTATATG GTGATGAAAA CAGTGATAAA
    GTGACACCAG GGACATTTAT ACCCTATTGT TCAATGGCAC ATGCACAGCT TTGCTTCCAT
    GGGCACAGGG ATGCTGTGAA ATTTTTTGTG GCTGTCCCAG GTCAAGTCAT CAGCCCACAA
    AGTGGCAGCA GTGGTACAGA TCTGACAGTT GACAAAGCAG GGCCATCTGC ACAGGAGCCT
    GGCATCCAGA CACCCTTGAA GTCTGTGCTT GTCATTAGTG GAGGAGAGGG CTACATTGAC
    TTCCGAATGG GTGATGAAGG TGGAGAATCA GAGCTTCTTG GAGAGGATCT TCCACTTGAA
    CCTTCTGTCG CCAAAGCAGA AAGGAGTCAC TTGATAGTGT GGCAAGTGAT GTATGGTAGT
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814461.1
    CDS378..3923
    Translation

    Target ORF information:

    RefSeq Version XM_010816159.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm associated antigen 9 (SPAG9), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816159.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
    AATTCTGCTA TCTTAGTATC TCTCTCCGTC TTGCTGCTTG TGCACTTCTC TATTTCTACC
    GGAGTGCCAG CTTTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG TCCTATATCG
    TTAGGGATTT TCCCTTTACC TTCTGGAGAC GGATTGCTTA CGCCTGACAC TCAGAAAGGT
    GGCGAGACCC CTGGATCAGA ACAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT
    ACCAGTCTGA AGGATGAACT TTCCGATGTT AGCCAGGGAG GATCTAAAGC CACCACTCCA
    GCGTCGACAG CTGCTTCAGA TGTGGCAGCA ACACCTAGCG ATACTCCCTT ACATGAGGAG
    AACGGAGGGG TTGTGGAGGT TGCAGATACA CCCGATAAGT CAGAGATAAG CAAGCATATC
    GAAGTACACG TAGTCCAGGA AACTAGAAAC ATAGCAACGG GTTCTGCTGA AAATGAAGAG
    AAGTCAGAAG TTCAGGCAAT CATTGAATCC ACTCCTGAGT TGGATATGGA CAAAGATCTC
    AGTGGATATA AAGGCTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC
    AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGTT CAGGTCTAAT TGGAGATGTG
    GATGAAGGAG CAGATTTACT AGGAATGGGT CGTGAAGTTG AAAATCTCAT ATTGGAAAAT
    ACACAACTAT TGGAAACCAA AAATGCCTTG AATGTAGTGA AGAATGACTT GATAGCAAAA
    GTAGATGAAC TTACCTGTGA GAAAGATGTG CTGCAAGGGG AGCTGGAGGC TGTGAAACAA
    GCCAAGCTGA AGCTGGAGGA AAAGAACAAA GAATTGGAAG AAGAGCTTAG GAAAGCTCGT
    GCAGAAGTTG AAGATGCAAA GCAAAAAGCA AAAGATGATG ATGATAGTGA TATTCCCACA
    GCCCAAAGGA AGCGGTTTAC TAGAGTAGAA ATGGCCCGAG TTCTAATGGA GAGAAACCAA
    TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGTGCG
    TCAAGAGAAA ATCCAGCCAT GCAGGAGAAG AAAAGGTCAA GCATTTGGCA GTTTATGCCA
    GCTCGTTTCA GCCGACTTTT CAGCTCCTCA AGTAACACTA CTAAGAAGCC CGAACCACCA
    GTTAATCTGA AGTACAATGC ACCAACCTCT CACGTTACGC CTTCCGTCAA GAAAAGAAGC
    AGCACCTTAT CTCAACTCCC TGGGGATAAG TCCAGAGCCT TTGATTTCCT TAGCGAAGAA
    ACTGAAGCTA GTTTAGCCTC ACGGAGAGAA CAAAAGAGAG AACAGTATCG TCAGGTAAAG
    GCCCATGTTC AGAAGGAAGA TGGTAGAGTG CAGGCTTTTG GCTGGAGTCT GCCTCAGAAA
    TACAAACAGG TAACCAACGG ACAAGGGGAA AATAAGATGA AAAATTTACC TGTGCCTGTC
    TATCTCAGAC CTCTAGATGA AAAAGATGCA TCAATGAAGC TTTGGTGTGC TGTTGGAGTC
    AATTTGTCTG GTGGGAAGAC CAGAGATGGT GGTTCTGTTG TTGGAGCAAG TGTGTTTTAC
    AAGGATGTTG CTGGTTTGGA AGCAGAAGGC AGTAAACAGC GAAGTGCATC TCAGAATAGC
    TTAGACAAGT TAGACCAGGA ACTTAAGGAA CAACAGAAGG AGTTAAAAAA TCAAGAAGAA
    TTATCCAGTC TAGTCTGGAT CTGTACCAGC ACTCATTCAG CTACAAAAGT TATCATTATC
    GATGCTGTTC AACCAGGCAA CATCCTAGAC AGCTTCACTG TTTGTAACTC TCACGTTCTG
    TGTATATCAA GCGTACCAGG AGCCCGAGAA ACAGACTACC CTGCAGGAGA AGAGCTTTCA
    GAATCTGGAC AAGTAGACAA AGCATCTTTA TGTGGAAGCA TGACAAGCAA TAGCTCAGCA
    GAGACAGACA GCCTGTTGGG AGGCATCACA GTAGTTGGTT GTTCTGCAGA AGGTGTGACA
    GCAGCTGCCA CTTCCCCTAA TACCAATGAA CCTTCTCCAG TGATGGAAAA ACCGCCAGAA
    AAGGAAACAG AAAATAGTGA GATTGATGAA AATGTTCCAA CAGCAGAAGA AGCAACTGAA
    GCTACAGAAG GCAATGCAGG ATCAGCTGAA GATACTGTGG ATGTCTCCCA AACTGGCGTG
    TACACAGAGC ATGTGTTTAC AGATCCTTTG GGAGTTCAAA TCCCAGAAGA CCTGTCACCA
    GTATATCAGT CAAGTAATGA CTCAGATGCA TATAAAGATC AAATATCAGT ATTGCCAAAT
    GAACAAGACC TGGTGAGAGA GGAAGCCCAG AAAATGAGTA GCCTTTTACC AACTATGTGG
    CTTGGAGCTC AAAATGGGTG TTTGTATGTC CATTCATCTG TAGCCCAGTG GAGGAAATGT
    CTCCATTCTA TTAAACTTAA AGATTCGATT CTCAGTATTG TACACGTGAA AGGAATTGTG
    TTAGTGGCCC TAGCTGATGG GACCCTTGCC ATCTTTCACA GAGGAGTTGA TGGACAGTGG
    GATTTGTCAA ACTATCACCT GTTAGATCTT GGACGACCGC ACCATTCCAT CCGATGCATG
    ACTGTGGTAC ATGACAAAGT TTGGTGTGGC TATAGGAACA AAATTTATGT GGTTCAGCCA
    AAGGCCATGA AAATAGAGAA ATCATTTGAT GCACACCCCA GGAAGGAGAG CCAAGTGCGG
    CAGCTTGCCT GGGTGGGTGA TGGTGTGTGG GTCTCCATTC GCTTGGATTC CACACTCCGT
    CTCTATCATG CACACACTTA TCAGCATCTG CAGGATGTGG ACATTGAGCC TTATGTAAGC
    AAAATGCTAG GTACTGGAAA ACTGGGCTTC TCTTTTGTGA GGATCACAGC GCTTATGGTG
    TCTTGTAACC GTTTGTGGGT GGGAACAGGA AATGGTGTCA TTATCTCCAT CCCATTGACA
    GAAACCGTAA TCCTCCACCA GGGACGTTTA CTGGGGCTGA GGGCGAATAA AACATCAGGA
    GCATCGGGAA ATCGTCCTGG AAGTGTCATT CGAGTATATG GTGATGAAAA CAGTGATAAA
    GTGACACCAG GGACATTTAT ACCCTATTGT TCAATGGCAC ATGCACAGCT TTGCTTCCAT
    GGGCACAGGG ATGCTGTGAA ATTTTTTGTG GCTGTCCCAG GTCAAGTCAT CAGCCCACAA
    AGTGGCAGCA GTGGTACAGA TCTGACAGTT GACAAAGCAG GGCCATCTGC ACAGGAGCCT
    GGCATCCAGA CACCCTTGAA GTCTGTGCTT GTCATTAGTG GAGGAGAGGG CTACATTGAC
    TTCCGAATGG GTGATGAAGG TGGAGAATCA GAGCTTCTTG GAGAGGATCT TCCACTTGAA
    CCTTCTGTCG CCAAAGCAGA AAGGAGTCAC TTGATAGTGT GGCAAGTGAT GTATGGTAGT
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.