KALRN cDNA ORF clone, Bos taurus(cattle)

The following KALRN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KALRN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa218904 XM_024991402.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa218905 XM_024991404.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa218906 XM_024991409.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa218903 XM_024991400.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa218897 XM_024991377.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa218898 XM_024991383.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa218899 XM_024991384.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa218900 XM_024991386.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa218901 XM_024991394.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa218902 XM_024991396.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa218894 XM_024991369.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa218895 XM_024991371.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa218896 XM_024991374.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa01748 NM_001191364.1
Latest version!
Bos taurus kalirin, RhoGEF kinase (KALRN), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa218890 XM_024991355.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa218891 XM_024991357.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa218892 XM_024991361.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa218893 XM_024991366.1
Latest version!
Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa218904
    Clone ID Related Accession (Same CDS sequence) XM_024991402.1
    Accession Version XM_024991402.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2103bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAGGGCG GCGACCGCGC GTACACCCGA GGTCCCTCTT TGGGGTGGCT CTTTGCGAAG 
    TGCTGCTGTT GCTTTCCGTG CAGAGATGCG TACTCTCATT CCTCAAGTGA AAACGGAGGC
    AAGTCCGAGT CGGTGGCCAA CCTGCAGGCC CAACCATCCC TGAACTCCAT CCACAGCTCC
    CCTGGGCCCA AGCGTTCCAC CAACACCCTG AAGAAGTGGC TGACGAGTCC CGTGCGCCGG
    CTTAACAGTG GGAAAGCAGA TGGAAACATC AAGAAGCAGA AGAAAGTACG AGATGGTCGG
    AAGAGCTTTG ACCTGGGATC TCCCAAGCCT GGGGATGAGA CGACCCCTCA GGGAGACAGC
    GCTGATGAGA GCAAGAAAGG TTGGGGTGAA GATGAGGCAG ATGAAGAGTC ACACACACCC
    CTCCCCCCGC CTATGAAGAT CTTTGACAAC GACCCCACGC AGGACGAAAT GTCCTCCTCT
    TTGCTAGCAG CCCGGCAGGC TTCCACCGAA GTACCTACTG CTGCAGACCT TGTCAGTGCG
    ATAGAACAGT TGGTCAAAAG CAAGCTGAGT CTGGAAGGAG GCTCTTACCG AGGGAGCTTG
    AAAGACCCCA CAGGCTGCCT GAATGAAGGC ATGACCCCAC CCACACCTCC CAGAAATCTG
    GAAGAAGAAC AGAAAGCCAA GGCCCTGAGA GGCAGGATGT TTGTCCTGAA TGAACTGGTA
    CAAACAGAGA AGGACTATGT CAAGGACCTG GGAATTGTGG TGGAGGGTTT CATGAAGAGA
    ATAGAAGAAA AGGGTGTCCC CGAGGACATG CGAGGAAAGG ATAAAATCGT GTTTGGAAAT
    ATTCACCAGA TTTATGATTG GCATAAGGAT GTTTTCCTGG GTGAACTGGA AAAATGTATC
    CAGGAGCAAG ACAGATTGGC ACAGCTCTTT ATTAAATATG AGCGGAAGCT GCACATCTAT
    GTGTGGTACT GCCAGAACAA GCCGCGCTCC GAGTACATCG TCGCCGAGTA CGACGCCTAC
    TTTGAAGAGG TGAAACAAGA GATAAATCAA AGGCTGACGC TTAGCGACTT CCTTATCAAG
    CCCATTCAGA GAATAACAAA ATATCAATTG CTCCTCAAGG ACTTCCTGAG ATACAGCGAG
    AAGGCTGGTT TGGAGTGTTC AGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTTCCA
    AAACGCTGCA ATGACATGAT GAACCTGGGG CGCCTGCAGG GCTTTGAGGG GACCCTGACT
    GCTCAGGGCA AGCTGCTGCA GCAGGACACA TTCTACGTGA TTGAGCTGGA CGCTGGCATG
    CAGTCGCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AAATCGTCAT CTTCAGTGAA
    CTGCTCAGGA AGGGATCCCT CACCCCAGGC TACATGTTCA AAAGGAGCAT CAAGATGAAC
    TACTTGGTCC TGGAGGAGAA CGTGGACAAT GACCCCTGCA AGTTTGCGCT TCTGAACAGG
    GAGACTTCAG AGAGGGTTAT TCTGCAAGCC GCCAATGCTG ACATCCAGCA GGCCTGGGTG
    CAGGACATCA ACCAAGTCTT AGAAACACAG CGAGACTTCT TAAATGCCCT ACAGTCGCCC
    ATTGAGTATC AGCGGAAAGA AAGGAGCACA GCCGTGATGA GGTCCCAGCC TGCTAGGGTT
    CCCCAAGCCA GCCCCAGGCC CTACTCCTCC ATCCCCGTGG GCTCCGAGAA GCCCCCAAAG
    GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCCAA TGGCAGTCCA
    GGGTTTGACT ACCACCAGGC TGGGGACAAG TTTGAGGCCA GCAAGAATGA CCTGGGAGGC
    TGCAACGGGG CCTCATCCAT GGCCGTGATC AAAGATTATT ATGCACTGAA GGAGAACGAA
    ATCTGTGTGA GCCAAGGTGA GGTGGTCCAG GTCCTCGCCG TCAACCAGCA GAACATGTGC
    CTGGTGTACC AGCCCGCTAG CGACCACTCC CCCGCCGCTG AGGGCTGGGT CCCTGGCAGC
    ATCCTGACGC CCCTCACCAA AGCCATGGCA GCCACAGAAA ATAGTGACGG GAGCATCAAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847170.1
    CDS1..2103
    Translation

    Target ORF information:

    RefSeq Version XM_024991402.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991402.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAGGGCG GCGACCGCGC GTACACCCGA GGTCCCTCTT TGGGGTGGCT CTTTGCGAAG 
    TGCTGCTGTT GCTTTCCGTG CAGAGATGCG TACTCTCATT CCTCAAGTGA AAACGGAGGC
    AAGTCCGAGT CGGTGGCCAA CCTGCAGGCC CAACCATCCC TGAACTCCAT CCACAGCTCC
    CCTGGGCCCA AGCGTTCCAC CAACACCCTG AAGAAGTGGC TGACGAGTCC CGTGCGCCGG
    CTTAACAGTG GGAAAGCAGA TGGAAACATC AAGAAGCAGA AGAAAGTACG AGATGGTCGG
    AAGAGCTTTG ACCTGGGATC TCCCAAGCCT GGGGATGAGA CGACCCCTCA GGGAGACAGC
    GCTGATGAGA GCAAGAAAGG TTGGGGTGAA GATGAGGCAG ATGAAGAGTC ACACACACCC
    CTCCCCCCGC CTATGAAGAT CTTTGACAAC GACCCCACGC AGGACGAAAT GTCCTCCTCT
    TTGCTAGCAG CCCGGCAGGC TTCCACCGAA GTACCTACTG CTGCAGACCT TGTCAGTGCG
    ATAGAACAGT TGGTCAAAAG CAAGCTGAGT CTGGAAGGAG GCTCTTACCG AGGGAGCTTG
    AAAGACCCCA CAGGCTGCCT GAATGAAGGC ATGACCCCAC CCACACCTCC CAGAAATCTG
    GAAGAAGAAC AGAAAGCCAA GGCCCTGAGA GGCAGGATGT TTGTCCTGAA TGAACTGGTA
    CAAACAGAGA AGGACTATGT CAAGGACCTG GGAATTGTGG TGGAGGGTTT CATGAAGAGA
    ATAGAAGAAA AGGGTGTCCC CGAGGACATG CGAGGAAAGG ATAAAATCGT GTTTGGAAAT
    ATTCACCAGA TTTATGATTG GCATAAGGAT GTTTTCCTGG GTGAACTGGA AAAATGTATC
    CAGGAGCAAG ACAGATTGGC ACAGCTCTTT ATTAAATATG AGCGGAAGCT GCACATCTAT
    GTGTGGTACT GCCAGAACAA GCCGCGCTCC GAGTACATCG TCGCCGAGTA CGACGCCTAC
    TTTGAAGAGG TGAAACAAGA GATAAATCAA AGGCTGACGC TTAGCGACTT CCTTATCAAG
    CCCATTCAGA GAATAACAAA ATATCAATTG CTCCTCAAGG ACTTCCTGAG ATACAGCGAG
    AAGGCTGGTT TGGAGTGTTC AGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTTCCA
    AAACGCTGCA ATGACATGAT GAACCTGGGG CGCCTGCAGG GCTTTGAGGG GACCCTGACT
    GCTCAGGGCA AGCTGCTGCA GCAGGACACA TTCTACGTGA TTGAGCTGGA CGCTGGCATG
    CAGTCGCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AAATCGTCAT CTTCAGTGAA
    CTGCTCAGGA AGGGATCCCT CACCCCAGGC TACATGTTCA AAAGGAGCAT CAAGATGAAC
    TACTTGGTCC TGGAGGAGAA CGTGGACAAT GACCCCTGCA AGTTTGCGCT TCTGAACAGG
    GAGACTTCAG AGAGGGTTAT TCTGCAAGCC GCCAATGCTG ACATCCAGCA GGCCTGGGTG
    CAGGACATCA ACCAAGTCTT AGAAACACAG CGAGACTTCT TAAATGCCCT ACAGTCGCCC
    ATTGAGTATC AGCGGAAAGA AAGGAGCACA GCCGTGATGA GGTCCCAGCC TGCTAGGGTT
    CCCCAAGCCA GCCCCAGGCC CTACTCCTCC ATCCCCGTGG GCTCCGAGAA GCCCCCAAAG
    GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCCAA TGGCAGTCCA
    GGGTTTGACT ACCACCAGGC TGGGGACAAG TTTGAGGCCA GCAAGAATGA CCTGGGAGGC
    TGCAACGGGG CCTCATCCAT GGCCGTGATC AAAGATTATT ATGCACTGAA GGAGAACGAA
    ATCTGTGTGA GCCAAGGTGA GGTGGTCCAG GTCCTCGCCG TCAACCAGCA GAACATGTGC
    CTGGTGTACC AGCCCGCTAG CGACCACTCC CCCGCCGCTG AGGGCTGGGT CCCTGGCAGC
    ATCCTGACGC CCCTCACCAA AGCCATGGCA GCCACAGAAA ATAGTGACGG GAGCATCAAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218905
    Clone ID Related Accession (Same CDS sequence) XM_024991404.1
    Accession Version XM_024991404.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAGGGCG GCGACCGCGC GTACACCCGA GGTCCCTCTT TGGGGTGGCT CTTTGCGAAG 
    TGCTGCTGTT GCTTTCCGTG CAGAGATGCG TACTCTCATT CCTCAAGTGA AAACGGAGGC
    AAGTCCGAGT CGGTGGCCAA CCTGCAGGCC CAACCATCCC TGAACTCCAT CCACAGCTCC
    CCTGGGCCCA AGCGTTCCAC CAACACCCTG AAGAAGTGGC TGACGAGTCC CGTGCGCCGG
    CTTAACAGTG GGAAAGCAGA TGGAAACATC AAGAAGCAGA AGAAAGTACG AGATGGTCGG
    AAGAGCTTTG ACCTGGGATC TCCCAAGCCT GGGGATGAGA CGACCCCTCA GGGAGACAGC
    GCTGATGAGA AGAGCAAGAA AGGTTGGGGT GAAGATGAGG CAGATGAAGA GTCACACACA
    CCCCTCCCCC CGCCTATGAA GATCTTTGAC AACGACCCCA CGCAGGACGA AATGAGTCTG
    GAAGGAGGCT CTTACCGAGG GAGCTTGAAA GACCCCACAG GCTGCCTGAA TGAAGGCATG
    ACCCCACCCA CACCTCCCAG AAATCTGGAA GAAGAACAGA AAGCCAAGGC CCTGAGAGGC
    AGGATGTTTG TCCTGAATGA ACTGGTACAA ACAGAGAAGG ACTATGTCAA GGACCTGGGA
    ATTGTGGTGG AGGGTTTCAT GAAGAGAATA GAAGAAAAGG GTGTCCCCGA GGACATGCGA
    GGAAAGGATA AAATCGTGTT TGGAAATATT CACCAGATTT ATGATTGGCA TAAGGATGTT
    TTCCTGGGTG AACTGGAAAA ATGTATCCAG GAGCAAGACA GATTGGCACA GCTCTTTATT
    AAATATGAGC GGAAGCTGCA CATCTATGTG TGGTACTGCC AGAACAAGCC GCGCTCCGAG
    TACATCGTCG CCGAGTACGA CGCCTACTTT GAAGAGGTGA AACAAGAGAT AAATCAAAGG
    CTGACGCTTA GCGACTTCCT TATCAAGCCC ATTCAGAGAA TAACAAAATA TCAATTGCTC
    CTCAAGGACT TCCTGAGATA CAGCGAGAAG GCTGGTTTGG AGTGTTCAGA TATTGAGAAA
    GCAGTGGAGT TAATGTGCCT TGTTCCAAAA CGCTGCAATG ACATGATGAA CCTGGGGCGC
    CTGCAGGGCT TTGAGGGGAC CCTGACTGCT CAGGGCAAGC TGCTGCAGCA GGACACATTC
    TACGTGATTG AGCTGGACGC TGGCATGCAG TCGCGGACCA AGGAGAGGCG TGTGTTTCTC
    TTTGAGCAAA TCGTCATCTT CAGTGAACTG CTCAGGAAGG GATCCCTCAC CCCAGGCTAC
    ATGTTCAAAA GGAGCATCAA GATGAACTAC TTGGTCCTGG AGGAGAACGT GGACAATGAC
    CCCTGCAAGT TTGCGCTTCT GAACAGGGAG ACTTCAGAGA GGGTTATTCT GCAAGCCGCC
    AATGCTGACA TCCAGCAGGC CTGGGTGCAG GACATCAACC AAGTCTTAGA AACACAGCGA
    GACTTCTTAA ATGCCCTACA GTCGCCCATT GAGTATCAGC GGAAAGAAAG GAGCACAGCC
    GTGATGAGGT CCCAGCCTGC TAGGGTTCCC CAAGCCAGCC CCAGGCCCTA CTCCTCCATC
    CCCGTGGGCT CCGAGAAGCC CCCAAAGGGC TCCAGCTATA ACCCGCCTCT GCCACCCCTG
    AAGATATCTA CCTCCAATGG CAGTCCAGGG TTTGACTACC ACCAGGCTGG GGACAAGTTT
    GAGGCCAGCA AGAATGACCT GGGAGGCTGC AACGGGGCCT CATCCATGGC CGTGATCAAA
    GATTATTATG CACTGAAGGA GAACGAAATC TGTGTGAGCC AAGGTGAGGT GGTCCAGGTC
    CTCGCCGTCA ACCAGCAGAA CATGTGCCTG GTGTACCAGC CCGCTAGCGA CCACTCCCCC
    GCCGCTGAGG GCTGGGTCCC TGGCAGCATC CTGACGCCCC TCACCAAAGC CATGGCAGCC
    ACAGAAAATA GTGACGGGAG CATCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847172.1
    CDS1..2010
    Translation

    Target ORF information:

    RefSeq Version XM_024991404.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991404.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAGGGCG GCGACCGCGC GTACACCCGA GGTCCCTCTT TGGGGTGGCT CTTTGCGAAG 
    TGCTGCTGTT GCTTTCCGTG CAGAGATGCG TACTCTCATT CCTCAAGTGA AAACGGAGGC
    AAGTCCGAGT CGGTGGCCAA CCTGCAGGCC CAACCATCCC TGAACTCCAT CCACAGCTCC
    CCTGGGCCCA AGCGTTCCAC CAACACCCTG AAGAAGTGGC TGACGAGTCC CGTGCGCCGG
    CTTAACAGTG GGAAAGCAGA TGGAAACATC AAGAAGCAGA AGAAAGTACG AGATGGTCGG
    AAGAGCTTTG ACCTGGGATC TCCCAAGCCT GGGGATGAGA CGACCCCTCA GGGAGACAGC
    GCTGATGAGA AGAGCAAGAA AGGTTGGGGT GAAGATGAGG CAGATGAAGA GTCACACACA
    CCCCTCCCCC CGCCTATGAA GATCTTTGAC AACGACCCCA CGCAGGACGA AATGAGTCTG
    GAAGGAGGCT CTTACCGAGG GAGCTTGAAA GACCCCACAG GCTGCCTGAA TGAAGGCATG
    ACCCCACCCA CACCTCCCAG AAATCTGGAA GAAGAACAGA AAGCCAAGGC CCTGAGAGGC
    AGGATGTTTG TCCTGAATGA ACTGGTACAA ACAGAGAAGG ACTATGTCAA GGACCTGGGA
    ATTGTGGTGG AGGGTTTCAT GAAGAGAATA GAAGAAAAGG GTGTCCCCGA GGACATGCGA
    GGAAAGGATA AAATCGTGTT TGGAAATATT CACCAGATTT ATGATTGGCA TAAGGATGTT
    TTCCTGGGTG AACTGGAAAA ATGTATCCAG GAGCAAGACA GATTGGCACA GCTCTTTATT
    AAATATGAGC GGAAGCTGCA CATCTATGTG TGGTACTGCC AGAACAAGCC GCGCTCCGAG
    TACATCGTCG CCGAGTACGA CGCCTACTTT GAAGAGGTGA AACAAGAGAT AAATCAAAGG
    CTGACGCTTA GCGACTTCCT TATCAAGCCC ATTCAGAGAA TAACAAAATA TCAATTGCTC
    CTCAAGGACT TCCTGAGATA CAGCGAGAAG GCTGGTTTGG AGTGTTCAGA TATTGAGAAA
    GCAGTGGAGT TAATGTGCCT TGTTCCAAAA CGCTGCAATG ACATGATGAA CCTGGGGCGC
    CTGCAGGGCT TTGAGGGGAC CCTGACTGCT CAGGGCAAGC TGCTGCAGCA GGACACATTC
    TACGTGATTG AGCTGGACGC TGGCATGCAG TCGCGGACCA AGGAGAGGCG TGTGTTTCTC
    TTTGAGCAAA TCGTCATCTT CAGTGAACTG CTCAGGAAGG GATCCCTCAC CCCAGGCTAC
    ATGTTCAAAA GGAGCATCAA GATGAACTAC TTGGTCCTGG AGGAGAACGT GGACAATGAC
    CCCTGCAAGT TTGCGCTTCT GAACAGGGAG ACTTCAGAGA GGGTTATTCT GCAAGCCGCC
    AATGCTGACA TCCAGCAGGC CTGGGTGCAG GACATCAACC AAGTCTTAGA AACACAGCGA
    GACTTCTTAA ATGCCCTACA GTCGCCCATT GAGTATCAGC GGAAAGAAAG GAGCACAGCC
    GTGATGAGGT CCCAGCCTGC TAGGGTTCCC CAAGCCAGCC CCAGGCCCTA CTCCTCCATC
    CCCGTGGGCT CCGAGAAGCC CCCAAAGGGC TCCAGCTATA ACCCGCCTCT GCCACCCCTG
    AAGATATCTA CCTCCAATGG CAGTCCAGGG TTTGACTACC ACCAGGCTGG GGACAAGTTT
    GAGGCCAGCA AGAATGACCT GGGAGGCTGC AACGGGGCCT CATCCATGGC CGTGATCAAA
    GATTATTATG CACTGAAGGA GAACGAAATC TGTGTGAGCC AAGGTGAGGT GGTCCAGGTC
    CTCGCCGTCA ACCAGCAGAA CATGTGCCTG GTGTACCAGC CCGCTAGCGA CCACTCCCCC
    GCCGCTGAGG GCTGGGTCCC TGGCAGCATC CTGACGCCCC TCACCAAAGC CATGGCAGCC
    ACAGAAAATA GTGACGGGAG CATCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218906
    Clone ID Related Accession (Same CDS sequence) XM_024991409.1
    Accession Version XM_024991409.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2007bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAGGGCG GCGACCGCGC GTACACCCGA GGTCCCTCTT TGGGGTGGCT CTTTGCGAAG 
    TGCTGCTGTT GCTTTCCGTG CAGAGATGCG TACTCTCATT CCTCAAGTGA AAACGGAGGC
    AAGTCCGAGT CGGTGGCCAA CCTGCAGGCC CAACCATCCC TGAACTCCAT CCACAGCTCC
    CCTGGGCCCA AGCGTTCCAC CAACACCCTG AAGAAGTGGC TGACGAGTCC CGTGCGCCGG
    CTTAACAGTG GGAAAGCAGA TGGAAACATC AAGAAGCAGA AGAAAGTACG AGATGGTCGG
    AAGAGCTTTG ACCTGGGATC TCCCAAGCCT GGGGATGAGA CGACCCCTCA GGGAGACAGC
    GCTGATGAGA GCAAGAAAGG TTGGGGTGAA GATGAGGCAG ATGAAGAGTC ACACACACCC
    CTCCCCCCGC CTATGAAGAT CTTTGACAAC GACCCCACGC AGGACGAAAT GAGTCTGGAA
    GGAGGCTCTT ACCGAGGGAG CTTGAAAGAC CCCACAGGCT GCCTGAATGA AGGCATGACC
    CCACCCACAC CTCCCAGAAA TCTGGAAGAA GAACAGAAAG CCAAGGCCCT GAGAGGCAGG
    ATGTTTGTCC TGAATGAACT GGTACAAACA GAGAAGGACT ATGTCAAGGA CCTGGGAATT
    GTGGTGGAGG GTTTCATGAA GAGAATAGAA GAAAAGGGTG TCCCCGAGGA CATGCGAGGA
    AAGGATAAAA TCGTGTTTGG AAATATTCAC CAGATTTATG ATTGGCATAA GGATGTTTTC
    CTGGGTGAAC TGGAAAAATG TATCCAGGAG CAAGACAGAT TGGCACAGCT CTTTATTAAA
    TATGAGCGGA AGCTGCACAT CTATGTGTGG TACTGCCAGA ACAAGCCGCG CTCCGAGTAC
    ATCGTCGCCG AGTACGACGC CTACTTTGAA GAGGTGAAAC AAGAGATAAA TCAAAGGCTG
    ACGCTTAGCG ACTTCCTTAT CAAGCCCATT CAGAGAATAA CAAAATATCA ATTGCTCCTC
    AAGGACTTCC TGAGATACAG CGAGAAGGCT GGTTTGGAGT GTTCAGATAT TGAGAAAGCA
    GTGGAGTTAA TGTGCCTTGT TCCAAAACGC TGCAATGACA TGATGAACCT GGGGCGCCTG
    CAGGGCTTTG AGGGGACCCT GACTGCTCAG GGCAAGCTGC TGCAGCAGGA CACATTCTAC
    GTGATTGAGC TGGACGCTGG CATGCAGTCG CGGACCAAGG AGAGGCGTGT GTTTCTCTTT
    GAGCAAATCG TCATCTTCAG TGAACTGCTC AGGAAGGGAT CCCTCACCCC AGGCTACATG
    TTCAAAAGGA GCATCAAGAT GAACTACTTG GTCCTGGAGG AGAACGTGGA CAATGACCCC
    TGCAAGTTTG CGCTTCTGAA CAGGGAGACT TCAGAGAGGG TTATTCTGCA AGCCGCCAAT
    GCTGACATCC AGCAGGCCTG GGTGCAGGAC ATCAACCAAG TCTTAGAAAC ACAGCGAGAC
    TTCTTAAATG CCCTACAGTC GCCCATTGAG TATCAGCGGA AAGAAAGGAG CACAGCCGTG
    ATGAGGTCCC AGCCTGCTAG GGTTCCCCAA GCCAGCCCCA GGCCCTACTC CTCCATCCCC
    GTGGGCTCCG AGAAGCCCCC AAAGGGCTCC AGCTATAACC CGCCTCTGCC ACCCCTGAAG
    ATATCTACCT CCAATGGCAG TCCAGGGTTT GACTACCACC AGGCTGGGGA CAAGTTTGAG
    GCCAGCAAGA ATGACCTGGG AGGCTGCAAC GGGGCCTCAT CCATGGCCGT GATCAAAGAT
    TATTATGCAC TGAAGGAGAA CGAAATCTGT GTGAGCCAAG GTGAGGTGGT CCAGGTCCTC
    GCCGTCAACC AGCAGAACAT GTGCCTGGTG TACCAGCCCG CTAGCGACCA CTCCCCCGCC
    GCTGAGGGCT GGGTCCCTGG CAGCATCCTG ACGCCCCTCA CCAAAGCCAT GGCAGCCACA
    GAAAATAGTG ACGGGAGCAT CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847177.1
    CDS1..2007
    Translation

    Target ORF information:

    RefSeq Version XM_024991409.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991409.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAGGGCG GCGACCGCGC GTACACCCGA GGTCCCTCTT TGGGGTGGCT CTTTGCGAAG 
    TGCTGCTGTT GCTTTCCGTG CAGAGATGCG TACTCTCATT CCTCAAGTGA AAACGGAGGC
    AAGTCCGAGT CGGTGGCCAA CCTGCAGGCC CAACCATCCC TGAACTCCAT CCACAGCTCC
    CCTGGGCCCA AGCGTTCCAC CAACACCCTG AAGAAGTGGC TGACGAGTCC CGTGCGCCGG
    CTTAACAGTG GGAAAGCAGA TGGAAACATC AAGAAGCAGA AGAAAGTACG AGATGGTCGG
    AAGAGCTTTG ACCTGGGATC TCCCAAGCCT GGGGATGAGA CGACCCCTCA GGGAGACAGC
    GCTGATGAGA GCAAGAAAGG TTGGGGTGAA GATGAGGCAG ATGAAGAGTC ACACACACCC
    CTCCCCCCGC CTATGAAGAT CTTTGACAAC GACCCCACGC AGGACGAAAT GAGTCTGGAA
    GGAGGCTCTT ACCGAGGGAG CTTGAAAGAC CCCACAGGCT GCCTGAATGA AGGCATGACC
    CCACCCACAC CTCCCAGAAA TCTGGAAGAA GAACAGAAAG CCAAGGCCCT GAGAGGCAGG
    ATGTTTGTCC TGAATGAACT GGTACAAACA GAGAAGGACT ATGTCAAGGA CCTGGGAATT
    GTGGTGGAGG GTTTCATGAA GAGAATAGAA GAAAAGGGTG TCCCCGAGGA CATGCGAGGA
    AAGGATAAAA TCGTGTTTGG AAATATTCAC CAGATTTATG ATTGGCATAA GGATGTTTTC
    CTGGGTGAAC TGGAAAAATG TATCCAGGAG CAAGACAGAT TGGCACAGCT CTTTATTAAA
    TATGAGCGGA AGCTGCACAT CTATGTGTGG TACTGCCAGA ACAAGCCGCG CTCCGAGTAC
    ATCGTCGCCG AGTACGACGC CTACTTTGAA GAGGTGAAAC AAGAGATAAA TCAAAGGCTG
    ACGCTTAGCG ACTTCCTTAT CAAGCCCATT CAGAGAATAA CAAAATATCA ATTGCTCCTC
    AAGGACTTCC TGAGATACAG CGAGAAGGCT GGTTTGGAGT GTTCAGATAT TGAGAAAGCA
    GTGGAGTTAA TGTGCCTTGT TCCAAAACGC TGCAATGACA TGATGAACCT GGGGCGCCTG
    CAGGGCTTTG AGGGGACCCT GACTGCTCAG GGCAAGCTGC TGCAGCAGGA CACATTCTAC
    GTGATTGAGC TGGACGCTGG CATGCAGTCG CGGACCAAGG AGAGGCGTGT GTTTCTCTTT
    GAGCAAATCG TCATCTTCAG TGAACTGCTC AGGAAGGGAT CCCTCACCCC AGGCTACATG
    TTCAAAAGGA GCATCAAGAT GAACTACTTG GTCCTGGAGG AGAACGTGGA CAATGACCCC
    TGCAAGTTTG CGCTTCTGAA CAGGGAGACT TCAGAGAGGG TTATTCTGCA AGCCGCCAAT
    GCTGACATCC AGCAGGCCTG GGTGCAGGAC ATCAACCAAG TCTTAGAAAC ACAGCGAGAC
    TTCTTAAATG CCCTACAGTC GCCCATTGAG TATCAGCGGA AAGAAAGGAG CACAGCCGTG
    ATGAGGTCCC AGCCTGCTAG GGTTCCCCAA GCCAGCCCCA GGCCCTACTC CTCCATCCCC
    GTGGGCTCCG AGAAGCCCCC AAAGGGCTCC AGCTATAACC CGCCTCTGCC ACCCCTGAAG
    ATATCTACCT CCAATGGCAG TCCAGGGTTT GACTACCACC AGGCTGGGGA CAAGTTTGAG
    GCCAGCAAGA ATGACCTGGG AGGCTGCAAC GGGGCCTCAT CCATGGCCGT GATCAAAGAT
    TATTATGCAC TGAAGGAGAA CGAAATCTGT GTGAGCCAAG GTGAGGTGGT CCAGGTCCTC
    GCCGTCAACC AGCAGAACAT GTGCCTGGTG TACCAGCCCG CTAGCGACCA CTCCCCCGCC
    GCTGAGGGCT GGGTCCCTGG CAGCATCCTG ACGCCCCTCA CCAAAGCCAT GGCAGCCACA
    GAAAATAGTG ACGGGAGCAT CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218903
    Clone ID Related Accession (Same CDS sequence) XM_024991400.1
    Accession Version XM_024991400.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGGCG GCGACCGCGC GTACACCCGA GGTCCCTCTT TGGGGTGGCT CTTTGCGAAG 
    TGCTGCTGTT GCTTTCCGTG CAGAGATGCG TACTCTCATT CCTCAAGTGA AAACGGAGGC
    AAGTCCGAGT CGGTGGCCAA CCTGCAGGCC CAACCATCCC TGAACTCCAT CCACAGCTCC
    CCTGGGCCCA AGCGTTCCAC CAACACCCTG AAGAAGTGGC TGACGAGTCC CGTGCGCCGG
    CTTAACAGTG GGAAAGCAGA TGGAAACATC AAGAAGCAGA AGAAAGTACG AGATGGTCGG
    AAGAGCTTTG ACCTGGGATC TCCCAAGCCT GGGGATGAGA CGACCCCTCA GGGAGACAGC
    GCTGATGAGA AGAGCAAGAA AGGTTGGGGT GAAGATGAGG CAGATGAAGA GTCACACACA
    CCCCTCCCCC CGCCTATGAA GATCTTTGAC AACGACCCCA CGCAGGACGA AATGTCCTCC
    TCTTTGCTAG CAGCCCGGCA GGCTTCCACC GAAGTACCTA CTGCTGCAGA CCTTGTCAGT
    GCGATAGAAC AGTTGGTCAA AAGCAAGCTG AGTCTGGAAG GAGGCTCTTA CCGAGGGAGC
    TTGAAAGACC CCACAGGCTG CCTGAATGAA GGCATGACCC CACCCACACC TCCCAGAAAT
    CTGGAAGAAG AACAGAAAGC CAAGGCCCTG AGAGGCAGGA TGTTTGTCCT GAATGAACTG
    GTACAAACAG AGAAGGACTA TGTCAAGGAC CTGGGAATTG TGGTGGAGGG TTTCATGAAG
    AGAATAGAAG AAAAGGGTGT CCCCGAGGAC ATGCGAGGAA AGGATAAAAT CGTGTTTGGA
    AATATTCACC AGATTTATGA TTGGCATAAG GATGTTTTCC TGGGTGAACT GGAAAAATGT
    ATCCAGGAGC AAGACAGATT GGCACAGCTC TTTATTAAAT ATGAGCGGAA GCTGCACATC
    TATGTGTGGT ACTGCCAGAA CAAGCCGCGC TCCGAGTACA TCGTCGCCGA GTACGACGCC
    TACTTTGAAG AGGTGAAACA AGAGATAAAT CAAAGGCTGA CGCTTAGCGA CTTCCTTATC
    AAGCCCATTC AGAGAATAAC AAAATATCAA TTGCTCCTCA AGGACTTCCT GAGATACAGC
    GAGAAGGCTG GTTTGGAGTG TTCAGATATT GAGAAAGCAG TGGAGTTAAT GTGCCTTGTT
    CCAAAACGCT GCAATGACAT GATGAACCTG GGGCGCCTGC AGGGCTTTGA GGGGACCCTG
    ACTGCTCAGG GCAAGCTGCT GCAGCAGGAC ACATTCTACG TGATTGAGCT GGACGCTGGC
    ATGCAGTCGC GGACCAAGGA GAGGCGTGTG TTTCTCTTTG AGCAAATCGT CATCTTCAGT
    GAACTGCTCA GGAAGGGATC CCTCACCCCA GGCTACATGT TCAAAAGGAG CATCAAGATG
    AACTACTTGG TCCTGGAGGA GAACGTGGAC AATGACCCCT GCAAGTTTGC GCTTCTGAAC
    AGGGAGACTT CAGAGAGGGT TATTCTGCAA GCCGCCAATG CTGACATCCA GCAGGCCTGG
    GTGCAGGACA TCAACCAAGT CTTAGAAACA CAGCGAGACT TCTTAAATGC CCTACAGTCG
    CCCATTGAGT ATCAGCGGAA AGAAAGGAGC ACAGCCGTGA TGAGGTCCCA GCCTGCTAGG
    GTTCCCCAAG CCAGCCCCAG GCCCTACTCC TCCATCCCCG TGGGCTCCGA GAAGCCCCCA
    AAGGGCTCCA GCTATAACCC GCCTCTGCCA CCCCTGAAGA TATCTACCTC CAATGGCAGT
    CCAGGGTTTG ACTACCACCA GGCTGGGGAC AAGTTTGAGG CCAGCAAGAA TGACCTGGGA
    GGCTGCAACG GGGCCTCATC CATGGCCGTG ATCAAAGATT ATTATGCACT GAAGGAGAAC
    GAAATCTGTG TGAGCCAAGG TGAGGTGGTC CAGGTCCTCG CCGTCAACCA GCAGAACATG
    TGCCTGGTGT ACCAGCCCGC TAGCGACCAC TCCCCCGCCG CTGAGGGCTG GGTCCCTGGC
    AGCATCCTGA CGCCCCTCAC CAAAGCCATG GCAGCCACAG AAAATAGTGA CGGGAGCATC
    AAGAAGTCAT GTTCATGGCA TACTCTACGC ATGAGAAAGC GGGCAGAAGT GGAGAACACG
    GGTAAAAATG AAGCCACAGG GCCTCGTAAA CCCAAGGATA TTCTGGGCAA CAAAGTCTCT
    GTTAAAGAGA CGAACAGTTC CGAGGAGTCA GAGTGTGATG ATCTTGACCC TAATACTAGC
    ATGGAGATCT TAAATCCAAA TTTCATCCAA GAAGTGGCGC CAGAATTCCT CGTGCCCTTA
    GTGGATGTGA CCTGCTTGCT TGGGGACACA GTGACTCTGC AGTGCAAAGT CTGTGGGCGG
    CCAAAGCCCA CCATCACTTG GAAGGGTCCA GACCAGAACA TCCTTGACAC TGACAACAGC
    TCAGCTACAT ACACCGTCTC CTCCTGTGAC TCTGGGGAAA TCACCCTGAA AATCTGCAAT
    CTGATGCCCC AAGACAGCGG TATCTATACC TGCATAGCAG CAAACGACCA TGGTACTGCG
    TCAACATCTG CTACTGTTAA AGTGCAAGGT GTTCCAGCGG CCCCTAACCG CCCCATTGCC
    CAAGAGAGAA GCTGCACTTC CGTGATCCTC CGCTGGCTGC CCCCATCCAG CACGGGAAAC
    TGCACCATTT CCGGTTACAC CGTGGAGTAC AGAGAGGAAG GTTCCCAGGC CTGGCAGCAG
    TCAGTAGCAT CGACCTTGGA CACCTACCTT GTCATTGAAG ACCTCAGTCC TGGGAGTCCT
    TATCAGTTCA GAGTCAGTGC CAGTAACCCC TGGGGGATCA GCCTGCCCAG CGAGCCCTCA
    GAATTCGTGC GACTTCCAGA TTACGACGCT GCTGCCGATG GTGCCACCAT TTCTTGGAAG
    GAAAATTTTG ATTCAGCTTA TACTGAGCTG AATGAAATTG GAAGAGGCCG GTTCTCCATA
    GTAAAGAAGT GCATTCACAA AGCTACTCGC AAAGATGTCG CTGTGAAATT TGTTAGCAAA
    AAAATGAAGA AAAAAGAGCA GGCTGCCCAC GAGGCTGCCC TGCTTCAGCA CCTGCAGCAC
    CCCCAATACA TCACTCTCCA TGACACCTAT GAGTCCCCCA CTTCCTACAT CCTCATTTTG
    GAACTGATGG ACGATGGCCG ACTCTTAGAC TATCTTATGA ATCATGATGA GCTGATGGAA
    GAAAAAGTAG CTTTCTACAT TCGAGACATC ATGGAAGCCC TGCAGTACCT TCATAACTGC
    AGAGTTGCAC ATTTGGACAT AAAGCCTGAA AACCTGCTCA TTGACCTACG GATTCCAGTG
    CCTCGAGTGA AGCTCATTGA CTTGGAGGAT GCTGTCCAGA TCTCAGGTCA CTTCCATATC
    CACCACCTTC TGGGGAACCC CGAGTTTGCT GCCCCAGAAG TGATCCAGGG CATCCCTGTA
    TCCCTGGGCA CCGACATCTG GAGCATCGGG GTTTTGACAT ACGTCATGCT GAGTGGGGTC
    TCCCCCTTCT TGGATGAGAG CAAGGAGGAG ACATGTATCA ATGTGTGCAG GGTGGACTTC
    AGCTTCCCCC ATGAGTACTT CTGCGGTGTG AGCAATGCTG CCAGAGATTT CATCAATGTG
    ATCCTACAGG AAGACTTTCG GAGGCGGCCC ACAGCAGCCA CCTGCCTGCA GCATCCGTGG
    CTGCAGCCCC ACAATGGCAG CTACTCCAAG ATCCCCCTGG ACACCTCCCG CTTGGCGTGC
    TTCATCGAGC GCCGCAAGCA TCAGAATGAT GTGCGCCCCA TTCCCAACGT CAAGAGCTAC
    ATTGTCAATC GGCTAAACCA AGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847168.1
    CDS178..4047
    Translation

    Target ORF information:

    RefSeq Version XM_024991400.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991400.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGGCG GCGACCGCGC GTACACCCGA GGTCCCTCTT TGGGGTGGCT CTTTGCGAAG 
    TGCTGCTGTT GCTTTCCGTG CAGAGATGCG TACTCTCATT CCTCAAGTGA AAACGGAGGC
    AAGTCCGAGT CGGTGGCCAA CCTGCAGGCC CAACCATCCC TGAACTCCAT CCACAGCTCC
    CCTGGGCCCA AGCGTTCCAC CAACACCCTG AAGAAGTGGC TGACGAGTCC CGTGCGCCGG
    CTTAACAGTG GGAAAGCAGA TGGAAACATC AAGAAGCAGA AGAAAGTACG AGATGGTCGG
    AAGAGCTTTG ACCTGGGATC TCCCAAGCCT GGGGATGAGA CGACCCCTCA GGGAGACAGC
    GCTGATGAGA AGAGCAAGAA AGGTTGGGGT GAAGATGAGG CAGATGAAGA GTCACACACA
    CCCCTCCCCC CGCCTATGAA GATCTTTGAC AACGACCCCA CGCAGGACGA AATGTCCTCC
    TCTTTGCTAG CAGCCCGGCA GGCTTCCACC GAAGTACCTA CTGCTGCAGA CCTTGTCAGT
    GCGATAGAAC AGTTGGTCAA AAGCAAGCTG AGTCTGGAAG GAGGCTCTTA CCGAGGGAGC
    TTGAAAGACC CCACAGGCTG CCTGAATGAA GGCATGACCC CACCCACACC TCCCAGAAAT
    CTGGAAGAAG AACAGAAAGC CAAGGCCCTG AGAGGCAGGA TGTTTGTCCT GAATGAACTG
    GTACAAACAG AGAAGGACTA TGTCAAGGAC CTGGGAATTG TGGTGGAGGG TTTCATGAAG
    AGAATAGAAG AAAAGGGTGT CCCCGAGGAC ATGCGAGGAA AGGATAAAAT CGTGTTTGGA
    AATATTCACC AGATTTATGA TTGGCATAAG GATGTTTTCC TGGGTGAACT GGAAAAATGT
    ATCCAGGAGC AAGACAGATT GGCACAGCTC TTTATTAAAT ATGAGCGGAA GCTGCACATC
    TATGTGTGGT ACTGCCAGAA CAAGCCGCGC TCCGAGTACA TCGTCGCCGA GTACGACGCC
    TACTTTGAAG AGGTGAAACA AGAGATAAAT CAAAGGCTGA CGCTTAGCGA CTTCCTTATC
    AAGCCCATTC AGAGAATAAC AAAATATCAA TTGCTCCTCA AGGACTTCCT GAGATACAGC
    GAGAAGGCTG GTTTGGAGTG TTCAGATATT GAGAAAGCAG TGGAGTTAAT GTGCCTTGTT
    CCAAAACGCT GCAATGACAT GATGAACCTG GGGCGCCTGC AGGGCTTTGA GGGGACCCTG
    ACTGCTCAGG GCAAGCTGCT GCAGCAGGAC ACATTCTACG TGATTGAGCT GGACGCTGGC
    ATGCAGTCGC GGACCAAGGA GAGGCGTGTG TTTCTCTTTG AGCAAATCGT CATCTTCAGT
    GAACTGCTCA GGAAGGGATC CCTCACCCCA GGCTACATGT TCAAAAGGAG CATCAAGATG
    AACTACTTGG TCCTGGAGGA GAACGTGGAC AATGACCCCT GCAAGTTTGC GCTTCTGAAC
    AGGGAGACTT CAGAGAGGGT TATTCTGCAA GCCGCCAATG CTGACATCCA GCAGGCCTGG
    GTGCAGGACA TCAACCAAGT CTTAGAAACA CAGCGAGACT TCTTAAATGC CCTACAGTCG
    CCCATTGAGT ATCAGCGGAA AGAAAGGAGC ACAGCCGTGA TGAGGTCCCA GCCTGCTAGG
    GTTCCCCAAG CCAGCCCCAG GCCCTACTCC TCCATCCCCG TGGGCTCCGA GAAGCCCCCA
    AAGGGCTCCA GCTATAACCC GCCTCTGCCA CCCCTGAAGA TATCTACCTC CAATGGCAGT
    CCAGGGTTTG ACTACCACCA GGCTGGGGAC AAGTTTGAGG CCAGCAAGAA TGACCTGGGA
    GGCTGCAACG GGGCCTCATC CATGGCCGTG ATCAAAGATT ATTATGCACT GAAGGAGAAC
    GAAATCTGTG TGAGCCAAGG TGAGGTGGTC CAGGTCCTCG CCGTCAACCA GCAGAACATG
    TGCCTGGTGT ACCAGCCCGC TAGCGACCAC TCCCCCGCCG CTGAGGGCTG GGTCCCTGGC
    AGCATCCTGA CGCCCCTCAC CAAAGCCATG GCAGCCACAG AAAATAGTGA CGGGAGCATC
    AAGAAGTCAT GTTCATGGCA TACTCTACGC ATGAGAAAGC GGGCAGAAGT GGAGAACACG
    GGTAAAAATG AAGCCACAGG GCCTCGTAAA CCCAAGGATA TTCTGGGCAA CAAAGTCTCT
    GTTAAAGAGA CGAACAGTTC CGAGGAGTCA GAGTGTGATG ATCTTGACCC TAATACTAGC
    ATGGAGATCT TAAATCCAAA TTTCATCCAA GAAGTGGCGC CAGAATTCCT CGTGCCCTTA
    GTGGATGTGA CCTGCTTGCT TGGGGACACA GTGACTCTGC AGTGCAAAGT CTGTGGGCGG
    CCAAAGCCCA CCATCACTTG GAAGGGTCCA GACCAGAACA TCCTTGACAC TGACAACAGC
    TCAGCTACAT ACACCGTCTC CTCCTGTGAC TCTGGGGAAA TCACCCTGAA AATCTGCAAT
    CTGATGCCCC AAGACAGCGG TATCTATACC TGCATAGCAG CAAACGACCA TGGTACTGCG
    TCAACATCTG CTACTGTTAA AGTGCAAGGT GTTCCAGCGG CCCCTAACCG CCCCATTGCC
    CAAGAGAGAA GCTGCACTTC CGTGATCCTC CGCTGGCTGC CCCCATCCAG CACGGGAAAC
    TGCACCATTT CCGGTTACAC CGTGGAGTAC AGAGAGGAAG GTTCCCAGGC CTGGCAGCAG
    TCAGTAGCAT CGACCTTGGA CACCTACCTT GTCATTGAAG ACCTCAGTCC TGGGAGTCCT
    TATCAGTTCA GAGTCAGTGC CAGTAACCCC TGGGGGATCA GCCTGCCCAG CGAGCCCTCA
    GAATTCGTGC GACTTCCAGA TTACGACGCT GCTGCCGATG GTGCCACCAT TTCTTGGAAG
    GAAAATTTTG ATTCAGCTTA TACTGAGCTG AATGAAATTG GAAGAGGCCG GTTCTCCATA
    GTAAAGAAGT GCATTCACAA AGCTACTCGC AAAGATGTCG CTGTGAAATT TGTTAGCAAA
    AAAATGAAGA AAAAAGAGCA GGCTGCCCAC GAGGCTGCCC TGCTTCAGCA CCTGCAGCAC
    CCCCAATACA TCACTCTCCA TGACACCTAT GAGTCCCCCA CTTCCTACAT CCTCATTTTG
    GAACTGATGG ACGATGGCCG ACTCTTAGAC TATCTTATGA ATCATGATGA GCTGATGGAA
    GAAAAAGTAG CTTTCTACAT TCGAGACATC ATGGAAGCCC TGCAGTACCT TCATAACTGC
    AGAGTTGCAC ATTTGGACAT AAAGCCTGAA AACCTGCTCA TTGACCTACG GATTCCAGTG
    CCTCGAGTGA AGCTCATTGA CTTGGAGGAT GCTGTCCAGA TCTCAGGTCA CTTCCATATC
    CACCACCTTC TGGGGAACCC CGAGTTTGCT GCCCCAGAAG TGATCCAGGG CATCCCTGTA
    TCCCTGGGCA CCGACATCTG GAGCATCGGG GTTTTGACAT ACGTCATGCT GAGTGGGGTC
    TCCCCCTTCT TGGATGAGAG CAAGGAGGAG ACATGTATCA ATGTGTGCAG GGTGGACTTC
    AGCTTCCCCC ATGAGTACTT CTGCGGTGTG AGCAATGCTG CCAGAGATTT CATCAATGTG
    ATCCTACAGG AAGACTTTCG GAGGCGGCCC ACAGCAGCCA CCTGCCTGCA GCATCCGTGG
    CTGCAGCCCC ACAATGGCAG CTACTCCAAG ATCCCCCTGG ACACCTCCCG CTTGGCGTGC
    TTCATCGAGC GCCGCAAGCA TCAGAATGAT GTGCGCCCCA TTCCCAACGT CAAGAGCTAC
    ATTGTCAATC GGCTAAACCA AGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218897
    Clone ID Related Accession (Same CDS sequence) XM_024991377.1
    Accession Version XM_024991377.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4998bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CACACCAGAG CGCCCTGCAG
    GTTCAGCAGA AAGCTGAGGC GCTGCTCCAG GCTGGTCACT ATGATGCAGA TGCCATCCGG
    GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAGCAACTCA TGCTCAAGAT GGAAGACCGA
    CTGAAACTGG TCAATGCCTC AGTGGCCTTT TATAAAACTT CTGAGCAGGT CTGTAGTGTC
    CTGGAGAGCT TAGAGCAAGA ATACCGAAGA GATGAGGACT GGTGTGGTGG ACGAGATAAA
    TTGGGGCCAG CAGCAGAGAT CGACCACGTC ATTCCACTCA TCAGTAAACA TTTGGAACAA
    AAGGAAGCCT TTCTTAAGGC CTGCACCCTG GCACGGCGGA ATGCTGAGGT CTTTCTCAAG
    TACATCCATA GGAACAACGT CAGCATGCCC AGTGCTGCCA GCCACACCCG AGGACCCGAA
    CAGCAAGTGA AAGCCATCCT GAGTGAGCTC TTACAGAGGG AGAACCGTGT CCTGCACTTC
    TGGACATTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ACGTGGTATT TGAGCGAAGC
    GCCAAGCAGG CACTAGACTG GATCCAAGAA ACAGGTGAAT ATTACCTCTC AACACATACC
    TCCACCGGGG AGACCACAGA GGAGACTCAG GAACTGCTGA AAGAATATGG GGAGTTCAGG
    GTGCCTGCCA AGCAAACAAA GGAGAAGGTG AAGCTTCTGA TTCAGCTGGC TGATAGCTTT
    GTGGAAAAAG GTCACATTCA TGCCACAGAG ATCAGGAAAT GGGTGACCAC AGTGGACAAG
    CATTACAGAG ACTTCTCACT GAGGATGGGA AAATACCGGT ACTCCCTGGA GAAGGCCCTA
    GGAGTCAACA CGGAGGATAA CAAGGACCTG GAGCTGGACA TCATCCCAGC AAGCCTTTCA
    GATCGTGAGG TCAAGCTCCG GGATGCCAAC CACGAGGTCA ATGAAGAGAA GCGGAAATCA
    GCCAGGAAAA AAGAATTCAT TATGGCAGAA TTGCTCCAGA CAGAGAAGGC TTATGTGAGG
    GACTTACATG AGTGCTTAGA GACCTACCTG TGGGAAATGA CCAGTGGTGT GGAGGAGATC
    CCCCCTGGGA TCCTTAATAA GGAGCATATC ATCTTTGGCA ACATCCAAGA GATCTACGAT
    TTTCATAACA ACATCTTCCT CAAAGAACTG GAGAAGTATG AGCAGCTGCC TGAGGATGTG
    GGACACTGCT TTGTTACTTG GGCAGACAAA TTTCAGATGT ACGTCACCTA CTGTAAAAAC
    AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCAGGCA CCTTCTTTGA TGAGATACAG
    CAGCGGCATG GTCTGGCCAA CTCCATTTCT TCCTACCTAA TTAAGCCTGT CCAGAGGATT
    ACCAAGTACC AGCTGCTCCT GAAGGAACTT TTAACTTGCT GTGAAGAAGG AAAAGGGGAG
    CTCAAGGAAG GCCTGGAGGT GATGCTTAGT GTCCCAAAGA GAGCCAACGA TGCCATGCAC
    GTCAGCATGC TGGAAGGGTT CGACGAGAAC CTGGACGTGC AGGGGGAGCT GATTTTACAA
    GATGCCTTTC AAGTGTGGGA CCCCAAGTCG CTGATCCGGA AAGGGCGGGA GCGACACCTA
    TTCCTCTTTG AGATCTCCTT GGTTTTCAGC AAGGAGATCA AAGACTCTTC AGGACACACA
    AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA ACACGTAGAG
    GGCGATCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA
    GTGCTGAAAG CCTCCAACAT AGAAACCAAG CAGGAGTGGA TCAAGAACAT TCGAGAGGTG
    ATTCAAGAAA GGATCATTCA CCTGAAAGGA GCTTTGAAGG AGCCAATTCA GCTCCCCAAA
    ACACCAGCCA AACAGAGGAA CAACAGTAAG AGGGATGGAG TGGAGGATGT TGACAGCCAG
    GGGGATGGGA GCAGCCAGCC AGACACCATC TCCATTGCCT CTAGGACCTC CCAGAACACA
    GCGGACAGTG ACAAGGATGG CAACCTTGCT CCTCGGTGGT ACCTGGGACC TGGAGATCCT
    TTCTCCACTT ACGTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847145.1
    CDS360..5357
    Translation

    Target ORF information:

    RefSeq Version XM_024991377.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991377.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CACACCAGAG CGCCCTGCAG
    GTTCAGCAGA AAGCTGAGGC GCTGCTCCAG GCTGGTCACT ATGATGCAGA TGCCATCCGG
    GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAGCAACTCA TGCTCAAGAT GGAAGACCGA
    CTGAAACTGG TCAATGCCTC AGTGGCCTTT TATAAAACTT CTGAGCAGGT CTGTAGTGTC
    CTGGAGAGCT TAGAGCAAGA ATACCGAAGA GATGAGGACT GGTGTGGTGG ACGAGATAAA
    TTGGGGCCAG CAGCAGAGAT CGACCACGTC ATTCCACTCA TCAGTAAACA TTTGGAACAA
    AAGGAAGCCT TTCTTAAGGC CTGCACCCTG GCACGGCGGA ATGCTGAGGT CTTTCTCAAG
    TACATCCATA GGAACAACGT CAGCATGCCC AGTGCTGCCA GCCACACCCG AGGACCCGAA
    CAGCAAGTGA AAGCCATCCT GAGTGAGCTC TTACAGAGGG AGAACCGTGT CCTGCACTTC
    TGGACATTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ACGTGGTATT TGAGCGAAGC
    GCCAAGCAGG CACTAGACTG GATCCAAGAA ACAGGTGAAT ATTACCTCTC AACACATACC
    TCCACCGGGG AGACCACAGA GGAGACTCAG GAACTGCTGA AAGAATATGG GGAGTTCAGG
    GTGCCTGCCA AGCAAACAAA GGAGAAGGTG AAGCTTCTGA TTCAGCTGGC TGATAGCTTT
    GTGGAAAAAG GTCACATTCA TGCCACAGAG ATCAGGAAAT GGGTGACCAC AGTGGACAAG
    CATTACAGAG ACTTCTCACT GAGGATGGGA AAATACCGGT ACTCCCTGGA GAAGGCCCTA
    GGAGTCAACA CGGAGGATAA CAAGGACCTG GAGCTGGACA TCATCCCAGC AAGCCTTTCA
    GATCGTGAGG TCAAGCTCCG GGATGCCAAC CACGAGGTCA ATGAAGAGAA GCGGAAATCA
    GCCAGGAAAA AAGAATTCAT TATGGCAGAA TTGCTCCAGA CAGAGAAGGC TTATGTGAGG
    GACTTACATG AGTGCTTAGA GACCTACCTG TGGGAAATGA CCAGTGGTGT GGAGGAGATC
    CCCCCTGGGA TCCTTAATAA GGAGCATATC ATCTTTGGCA ACATCCAAGA GATCTACGAT
    TTTCATAACA ACATCTTCCT CAAAGAACTG GAGAAGTATG AGCAGCTGCC TGAGGATGTG
    GGACACTGCT TTGTTACTTG GGCAGACAAA TTTCAGATGT ACGTCACCTA CTGTAAAAAC
    AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCAGGCA CCTTCTTTGA TGAGATACAG
    CAGCGGCATG GTCTGGCCAA CTCCATTTCT TCCTACCTAA TTAAGCCTGT CCAGAGGATT
    ACCAAGTACC AGCTGCTCCT GAAGGAACTT TTAACTTGCT GTGAAGAAGG AAAAGGGGAG
    CTCAAGGAAG GCCTGGAGGT GATGCTTAGT GTCCCAAAGA GAGCCAACGA TGCCATGCAC
    GTCAGCATGC TGGAAGGGTT CGACGAGAAC CTGGACGTGC AGGGGGAGCT GATTTTACAA
    GATGCCTTTC AAGTGTGGGA CCCCAAGTCG CTGATCCGGA AAGGGCGGGA GCGACACCTA
    TTCCTCTTTG AGATCTCCTT GGTTTTCAGC AAGGAGATCA AAGACTCTTC AGGACACACA
    AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA ACACGTAGAG
    GGCGATCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA
    GTGCTGAAAG CCTCCAACAT AGAAACCAAG CAGGAGTGGA TCAAGAACAT TCGAGAGGTG
    ATTCAAGAAA GGATCATTCA CCTGAAAGGA GCTTTGAAGG AGCCAATTCA GCTCCCCAAA
    ACACCAGCCA AACAGAGGAA CAACAGTAAG AGGGATGGAG TGGAGGATGT TGACAGCCAG
    GGGGATGGGA GCAGCCAGCC AGACACCATC TCCATTGCCT CTAGGACCTC CCAGAACACA
    GCGGACAGTG ACAAGGATGG CAACCTTGCT CCTCGGTGGT ACCTGGGACC TGGAGATCCT
    TTCTCCACTT ACGTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218898
    Clone ID Related Accession (Same CDS sequence) XM_024991383.1
    Accession Version XM_024991383.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGGACC GCTTCTGGGA ACAGTGGTAT CTTTGGTATC TCCGCTTGCT CCGGCTGCTG 
    GATCGAGGCT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC CATCCTAAAG
    GAAAAAGTGG CCTTCGTGTC TGGGGGCCGT GATAAGCGAG GTGGACCCAT CCTGACCTTC
    CCTGCTCGCA GCAATCATGA CAGAATAAGA CAGGAAGACC TGCGGAAACT GGTGACCTAT
    CTGGCCAGCG TGCCAAGCGA GGACGTGTGC AAACGTGGCT TCACTGTCAT CATCGATATG
    CGGGGGTCCA AGTGGGACCT CATCAAGCCC CTCCTCAAGA CGCTGCAAGA AGCCTTTCCC
    GCTGAGATCC ATGTGGCCCT CATCATCAAA CCCGACAACT TCTGGCAGAA ACAGAAGACC
    AACTTTGGCA GCTCCAAGTT CATCTTTGAG ACAAGCATGG TGTCCGTGGA GGGCCTCACG
    AAGCTGGTGG ACCCCTCCCA GCTGACGGAG GAGTTCGATG GCTCCCTGGA CTACAACCAC
    GAGGAGTGGA TCGAGCTGCG GCTCTCCCTG GAGGAGTTCT TCAACAGCGC CGTACACCTG
    CTCTCACGCC TTGAAGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC CGTGGATGTG
    GAGGGCTCGA GGCGGCTAAT TGATGAACAC ACACAGCTGA AGAAGAAGGT GCTGAAGGCG
    CCTGTGGAGG AGCTGGACCG GGAGGGGCAG CGACTGCTGC AGTGCATCCG CTGCAGTGAC
    GGCTTCTCAG GGCGCAACTG CATCCCGGGC AGTGCCGACT TCCAGAGCTT GGTGCCCAAG
    ATCACCAGCC TGTTGGACAA GCTGCACTCC ACCCGGCAGC ACCTGCACCA GATGTGGCAT
    GTGCGCAAGC TCAAGCTAGA CCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAA
    AAGATGTTTG ACTGGATAAG CCACAACAAG GAGTTATTCC TCCAGAGCCA TACAGAGATT
    GGAGTCAGCT ACCAGCACGC CCTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC
    TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TGGCTTCCCG CCTCTCAGAG
    GCTGGCCACT ATGCTTCACA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG
    AAGAGTTTCG CAGCTGCCCT GGATGAACGC AGCACCATCC TCGCCATGTC TGCTGTGTTC
    CATCAGAAGG CTGAGCAGTT CTTGTCAGGA GTGGATGCCT GGTGCAAGAT GTGCAGTGAA
    GGTGGCCTGC CCTCTGAGAT GCAGGACCTG GAGCTGGCGA TCCACCACCA CCAGTCTTTG
    TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ACGGCAAAGC ACTGCTGGAT
    GTGCTGCAGC GGCCCCTGAG TCCCGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC
    TCCAAGGCGG TGCACCAGGT GCTGGACGTG GTGCACGAAG TGCTGCACCA CCAGCGGCGG
    CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTCCACC AACGCCTGCA GCTCTGCGTC
    TTCCAGCAGG ACGTCCAGCA GGTGTTAGAT TGGATTGAAA ACCACGGCGA GGCCTTTCTC
    AGCAAGCACA CAGGGGTCGG GAAGTCTCTG CACCGAGCTC GGGCCCTGCA GAAGAGGCAC
    GATGACTTTG AAGAGGTGGC TCAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCG
    GCAGAGCAGC TGGCTCAGAC AGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCTAGA
    CACCTGGAGG TGCGCATCCA AGACTTCGTG CGCAGGGTGG AGCAGCGGAA GCTTCTGCTG
    GACATGTCTG TCTCCTTCCA TACTCACACC AAAGAGCTAT GGACATGGAT GGAAGACCTT
    CAGAAGGAGA TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAACTGATC
    AAGCAGTTCC AGCAGCAGCA GACCGCCACT CTGGATGCCA CACTTAATGT CATCAAGGAG
    GGCGAAGACC TTATCCAGCA ACTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCAGCGAG
    GCCAGGGACT CAGCTGTGTC CAACAACAAG ACGCCCCATA GTAGCTCCAT CAGCCACATA
    GAGTCGGTGC TGCAGCAGCT GGATGACGCC CAGGTGCAAA TGGAGGAGCT GTTCCACGAA
    CGCAAGATCA AGCTGGACAT CTTCCTGCAG CTGCGCATCT TTGAGCAGTA CACCATTGAG
    GTGACAGCAG AGCTCGACGC TTGGAATGAG GATTTGCTTC GGCAGATGAA TGACTTCAAC
    ACGGAGGATC TAACACTGGC GGAACAGCGG CTGCAGCGCC ACACAGAGCG GAAGCTAGCC
    ATGAACAACA TGACCTTTGA GGTCATCCAG CAAGGCCAGG ATCTGCACCA GTACATCATG
    GAGGTCCAGG CCTCAGGGAT TGAGTTAATC TGTGAAAAAG ACATTGATCT GGCAGCCCAG
    GTACAAGAGC TATTAGAGTT TCTCCATGAG AAACAGCATG AGTTGGAGCT CAACGCAGAG
    CAGACCCACA AGCGACTTGA GCAGTGCCTC CAACTGCGGC ACCTCCAGGC CGAGGTCAAA
    CAGGTCCTAG GATGGATCCG CAATGGGGAG TCAATGCTCA ATGCCAGCCT GGTCAACGCC
    AGCTCTTTGT CTGAAGCAGA GCAGCTACAG CGGGAGCACG AGCAGTTCCA ACTAGCCATC
    GAGTCCCTCT TTCATGCCAC TTCCTTGCAG AAGACACACC AGAGCGCCCT GCAGGTTCAG
    CAGAAAGCTG AGGCGCTGCT CCAGGCTGGT CACTATGATG CAGATGCCAT CCGGGAATGT
    GCTGAGAAGG TGGCCCTCCA CTGGCAGCAA CTCATGCTCA AGATGGAAGA CCGACTGAAA
    CTGGTCAATG CCTCAGTGGC CTTTTATAAA ACTTCTGAGC AGGTCTGTAG TGTCCTGGAG
    AGCTTAGAGC AAGAATACCG AAGAGATGAG GACTGGTGTG GTGGACGAGA TAAATTGGGG
    CCAGCAGCAG AGATCGACCA CGTCATTCCA CTCATCAGTA AACATTTGGA ACAAAAGGAA
    GCCTTTCTTA AGGCCTGCAC CCTGGCACGG CGGAATGCTG AGGTCTTTCT CAAGTACATC
    CATAGGAACA ACGTCAGCAT GCCCAGTGCT GCCAGCCACA CCCGAGGACC CGAACAGCAA
    GTGAAAGCCA TCCTGAGTGA GCTCTTACAG AGGGAGAACC GTGTCCTGCA CTTCTGGACA
    TTGAAGAAGC GGCGGTTAGA CCAGTGTCAG CAATACGTGG TATTTGAGCG AAGCGCCAAG
    CAGGCACTAG ACTGGATCCA AGAAACAGGT GAATATTACC TCTCAACACA TACCTCCACC
    GGGGAGACCA CAGAGGAGAC TCAGGAACTG CTGAAAGAAT ATGGGGAGTT CAGGGTGCCT
    GCCAAGCAAA CAAAGGAGAA GGTGAAGCTT CTGATTCAGC TGGCTGATAG CTTTGTGGAA
    AAAGGTCACA TTCATGCCAC AGAGATCAGG AAATGGGTGA CCACAGTGGA CAAGCATTAC
    AGAGACTTCT CACTGAGGAT GGGAAAATAC CGGTACTCCC TGGAGAAGGC CCTAGGAGTC
    AACACGGAGG ATAACAAGGA CCTGGAGCTG GACATCATCC CAGCAAGCCT TTCAGATCGT
    GAGGTCAAGC TCCGGGATGC CAACCACGAG GTCAATGAAG AGAAGCGGAA ATCAGCCAGG
    AAAAAAGAAT TCATTATGGC AGAATTGCTC CAGACAGAGA AGGCTTATGT GAGGGACTTA
    CATGAGTGCT TAGAGACCTA CCTGTGGGAA ATGACCAGTG GTGTGGAGGA GATCCCCCCT
    GGGATCCTTA ATAAGGAGCA TATCATCTTT GGCAACATCC AAGAGATCTA CGATTTTCAT
    AACAACATCT TCCTCAAAGA ACTGGAGAAG TATGAGCAGC TGCCTGAGGA TGTGGGACAC
    TGCTTTGTTA CTTGGGCAGA CAAATTTCAG ATGTACGTCA CCTACTGTAA AAACAAGCCT
    GATTCCAACC AGCTGATCCT GGAGCATGCA GGCACCTTCT TTGATGAGAT ACAGCAGCGG
    CATGGTCTGG CCAACTCCAT TTCTTCCTAC CTAATTAAGC CTGTCCAGAG GATTACCAAG
    TACCAGCTGC TCCTGAAGGA ACTTTTAACT TGCTGTGAAG AAGGAAAAGG GGAGCTCAAG
    GAAGGCCTGG AGGTGATGCT TAGTGTCCCA AAGAGAGCCA ACGATGCCAT GCACGTCAGC
    ATGCTGGAAG GGTTCGACGA GAACCTGGAC GTGCAGGGGG AGCTGATTTT ACAAGATGCC
    TTTCAAGTGT GGGACCCCAA GTCGCTGATC CGGAAAGGGC GGGAGCGACA CCTATTCCTC
    TTTGAGATCT CCTTGGTTTT CAGCAAGGAG ATCAAAGACT CTTCAGGACA CACAAAATAT
    GTTTACAAGA ACAAGCTACT GACCTCAGAG CTGGGTGTGA CCGAACACGT AGAGGGCGAT
    CCCTGCAAAT TCGCCTTGTG GTCTGGGCGC ACCCCATCCT CAGACAATAA AACAGTGCTG
    AAAGCCTCCA ACATAGAAAC CAAGCAGGAG TGGATCAAGA ACATTCGAGA GGTGATTCAA
    GAAAGGATCA TTCACCTGAA AGGAGCTTTG AAGGAGCCAA TTCAGCTCCC CAAAACACCA
    GCCAAACAGA GGAACAACAG TAAGAGGGAT GGAGTGGAGG ATGTTGACAG CCAGGGGGAT
    GGGAGCAGCC AGCCAGACAC CATCTCCATT GCCTCTAGGA CCTCCCAGAA CACAGCGGAC
    AGTGACAAGG ATGGCAACCT TGCTCCTCGG TGGTACCTGG GACCTGGAGA TCCTTTCTCC
    ACTTACGTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847151.1
    CDS5083..10074
    Translation

    Target ORF information:

    RefSeq Version XM_024991383.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991383.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGGACC GCTTCTGGGA ACAGTGGTAT CTTTGGTATC TCCGCTTGCT CCGGCTGCTG 
    GATCGAGGCT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC CATCCTAAAG
    GAAAAAGTGG CCTTCGTGTC TGGGGGCCGT GATAAGCGAG GTGGACCCAT CCTGACCTTC
    CCTGCTCGCA GCAATCATGA CAGAATAAGA CAGGAAGACC TGCGGAAACT GGTGACCTAT
    CTGGCCAGCG TGCCAAGCGA GGACGTGTGC AAACGTGGCT TCACTGTCAT CATCGATATG
    CGGGGGTCCA AGTGGGACCT CATCAAGCCC CTCCTCAAGA CGCTGCAAGA AGCCTTTCCC
    GCTGAGATCC ATGTGGCCCT CATCATCAAA CCCGACAACT TCTGGCAGAA ACAGAAGACC
    AACTTTGGCA GCTCCAAGTT CATCTTTGAG ACAAGCATGG TGTCCGTGGA GGGCCTCACG
    AAGCTGGTGG ACCCCTCCCA GCTGACGGAG GAGTTCGATG GCTCCCTGGA CTACAACCAC
    GAGGAGTGGA TCGAGCTGCG GCTCTCCCTG GAGGAGTTCT TCAACAGCGC CGTACACCTG
    CTCTCACGCC TTGAAGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC CGTGGATGTG
    GAGGGCTCGA GGCGGCTAAT TGATGAACAC ACACAGCTGA AGAAGAAGGT GCTGAAGGCG
    CCTGTGGAGG AGCTGGACCG GGAGGGGCAG CGACTGCTGC AGTGCATCCG CTGCAGTGAC
    GGCTTCTCAG GGCGCAACTG CATCCCGGGC AGTGCCGACT TCCAGAGCTT GGTGCCCAAG
    ATCACCAGCC TGTTGGACAA GCTGCACTCC ACCCGGCAGC ACCTGCACCA GATGTGGCAT
    GTGCGCAAGC TCAAGCTAGA CCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAA
    AAGATGTTTG ACTGGATAAG CCACAACAAG GAGTTATTCC TCCAGAGCCA TACAGAGATT
    GGAGTCAGCT ACCAGCACGC CCTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC
    TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TGGCTTCCCG CCTCTCAGAG
    GCTGGCCACT ATGCTTCACA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG
    AAGAGTTTCG CAGCTGCCCT GGATGAACGC AGCACCATCC TCGCCATGTC TGCTGTGTTC
    CATCAGAAGG CTGAGCAGTT CTTGTCAGGA GTGGATGCCT GGTGCAAGAT GTGCAGTGAA
    GGTGGCCTGC CCTCTGAGAT GCAGGACCTG GAGCTGGCGA TCCACCACCA CCAGTCTTTG
    TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ACGGCAAAGC ACTGCTGGAT
    GTGCTGCAGC GGCCCCTGAG TCCCGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC
    TCCAAGGCGG TGCACCAGGT GCTGGACGTG GTGCACGAAG TGCTGCACCA CCAGCGGCGG
    CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTCCACC AACGCCTGCA GCTCTGCGTC
    TTCCAGCAGG ACGTCCAGCA GGTGTTAGAT TGGATTGAAA ACCACGGCGA GGCCTTTCTC
    AGCAAGCACA CAGGGGTCGG GAAGTCTCTG CACCGAGCTC GGGCCCTGCA GAAGAGGCAC
    GATGACTTTG AAGAGGTGGC TCAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCG
    GCAGAGCAGC TGGCTCAGAC AGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCTAGA
    CACCTGGAGG TGCGCATCCA AGACTTCGTG CGCAGGGTGG AGCAGCGGAA GCTTCTGCTG
    GACATGTCTG TCTCCTTCCA TACTCACACC AAAGAGCTAT GGACATGGAT GGAAGACCTT
    CAGAAGGAGA TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAACTGATC
    AAGCAGTTCC AGCAGCAGCA GACCGCCACT CTGGATGCCA CACTTAATGT CATCAAGGAG
    GGCGAAGACC TTATCCAGCA ACTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCAGCGAG
    GCCAGGGACT CAGCTGTGTC CAACAACAAG ACGCCCCATA GTAGCTCCAT CAGCCACATA
    GAGTCGGTGC TGCAGCAGCT GGATGACGCC CAGGTGCAAA TGGAGGAGCT GTTCCACGAA
    CGCAAGATCA AGCTGGACAT CTTCCTGCAG CTGCGCATCT TTGAGCAGTA CACCATTGAG
    GTGACAGCAG AGCTCGACGC TTGGAATGAG GATTTGCTTC GGCAGATGAA TGACTTCAAC
    ACGGAGGATC TAACACTGGC GGAACAGCGG CTGCAGCGCC ACACAGAGCG GAAGCTAGCC
    ATGAACAACA TGACCTTTGA GGTCATCCAG CAAGGCCAGG ATCTGCACCA GTACATCATG
    GAGGTCCAGG CCTCAGGGAT TGAGTTAATC TGTGAAAAAG ACATTGATCT GGCAGCCCAG
    GTACAAGAGC TATTAGAGTT TCTCCATGAG AAACAGCATG AGTTGGAGCT CAACGCAGAG
    CAGACCCACA AGCGACTTGA GCAGTGCCTC CAACTGCGGC ACCTCCAGGC CGAGGTCAAA
    CAGGTCCTAG GATGGATCCG CAATGGGGAG TCAATGCTCA ATGCCAGCCT GGTCAACGCC
    AGCTCTTTGT CTGAAGCAGA GCAGCTACAG CGGGAGCACG AGCAGTTCCA ACTAGCCATC
    GAGTCCCTCT TTCATGCCAC TTCCTTGCAG AAGACACACC AGAGCGCCCT GCAGGTTCAG
    CAGAAAGCTG AGGCGCTGCT CCAGGCTGGT CACTATGATG CAGATGCCAT CCGGGAATGT
    GCTGAGAAGG TGGCCCTCCA CTGGCAGCAA CTCATGCTCA AGATGGAAGA CCGACTGAAA
    CTGGTCAATG CCTCAGTGGC CTTTTATAAA ACTTCTGAGC AGGTCTGTAG TGTCCTGGAG
    AGCTTAGAGC AAGAATACCG AAGAGATGAG GACTGGTGTG GTGGACGAGA TAAATTGGGG
    CCAGCAGCAG AGATCGACCA CGTCATTCCA CTCATCAGTA AACATTTGGA ACAAAAGGAA
    GCCTTTCTTA AGGCCTGCAC CCTGGCACGG CGGAATGCTG AGGTCTTTCT CAAGTACATC
    CATAGGAACA ACGTCAGCAT GCCCAGTGCT GCCAGCCACA CCCGAGGACC CGAACAGCAA
    GTGAAAGCCA TCCTGAGTGA GCTCTTACAG AGGGAGAACC GTGTCCTGCA CTTCTGGACA
    TTGAAGAAGC GGCGGTTAGA CCAGTGTCAG CAATACGTGG TATTTGAGCG AAGCGCCAAG
    CAGGCACTAG ACTGGATCCA AGAAACAGGT GAATATTACC TCTCAACACA TACCTCCACC
    GGGGAGACCA CAGAGGAGAC TCAGGAACTG CTGAAAGAAT ATGGGGAGTT CAGGGTGCCT
    GCCAAGCAAA CAAAGGAGAA GGTGAAGCTT CTGATTCAGC TGGCTGATAG CTTTGTGGAA
    AAAGGTCACA TTCATGCCAC AGAGATCAGG AAATGGGTGA CCACAGTGGA CAAGCATTAC
    AGAGACTTCT CACTGAGGAT GGGAAAATAC CGGTACTCCC TGGAGAAGGC CCTAGGAGTC
    AACACGGAGG ATAACAAGGA CCTGGAGCTG GACATCATCC CAGCAAGCCT TTCAGATCGT
    GAGGTCAAGC TCCGGGATGC CAACCACGAG GTCAATGAAG AGAAGCGGAA ATCAGCCAGG
    AAAAAAGAAT TCATTATGGC AGAATTGCTC CAGACAGAGA AGGCTTATGT GAGGGACTTA
    CATGAGTGCT TAGAGACCTA CCTGTGGGAA ATGACCAGTG GTGTGGAGGA GATCCCCCCT
    GGGATCCTTA ATAAGGAGCA TATCATCTTT GGCAACATCC AAGAGATCTA CGATTTTCAT
    AACAACATCT TCCTCAAAGA ACTGGAGAAG TATGAGCAGC TGCCTGAGGA TGTGGGACAC
    TGCTTTGTTA CTTGGGCAGA CAAATTTCAG ATGTACGTCA CCTACTGTAA AAACAAGCCT
    GATTCCAACC AGCTGATCCT GGAGCATGCA GGCACCTTCT TTGATGAGAT ACAGCAGCGG
    CATGGTCTGG CCAACTCCAT TTCTTCCTAC CTAATTAAGC CTGTCCAGAG GATTACCAAG
    TACCAGCTGC TCCTGAAGGA ACTTTTAACT TGCTGTGAAG AAGGAAAAGG GGAGCTCAAG
    GAAGGCCTGG AGGTGATGCT TAGTGTCCCA AAGAGAGCCA ACGATGCCAT GCACGTCAGC
    ATGCTGGAAG GGTTCGACGA GAACCTGGAC GTGCAGGGGG AGCTGATTTT ACAAGATGCC
    TTTCAAGTGT GGGACCCCAA GTCGCTGATC CGGAAAGGGC GGGAGCGACA CCTATTCCTC
    TTTGAGATCT CCTTGGTTTT CAGCAAGGAG ATCAAAGACT CTTCAGGACA CACAAAATAT
    GTTTACAAGA ACAAGCTACT GACCTCAGAG CTGGGTGTGA CCGAACACGT AGAGGGCGAT
    CCCTGCAAAT TCGCCTTGTG GTCTGGGCGC ACCCCATCCT CAGACAATAA AACAGTGCTG
    AAAGCCTCCA ACATAGAAAC CAAGCAGGAG TGGATCAAGA ACATTCGAGA GGTGATTCAA
    GAAAGGATCA TTCACCTGAA AGGAGCTTTG AAGGAGCCAA TTCAGCTCCC CAAAACACCA
    GCCAAACAGA GGAACAACAG TAAGAGGGAT GGAGTGGAGG ATGTTGACAG CCAGGGGGAT
    GGGAGCAGCC AGCCAGACAC CATCTCCATT GCCTCTAGGA CCTCCCAGAA CACAGCGGAC
    AGTGACAAGG ATGGCAACCT TGCTCCTCGG TGGTACCTGG GACCTGGAGA TCCTTTCTCC
    ACTTACGTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218899
    Clone ID Related Accession (Same CDS sequence) XM_024991384.1
    Accession Version XM_024991384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGA AGACACACCA GAGCGCCCTG CAGGTTCAGC AGAAAGCTGA GGCGCTGCTC
    CAGGCTGGTC ACTATGATGC AGATGCCATC CGGGAATGTG CTGAGAAGGT GGCCCTCCAC
    TGGCAGCAAC TCATGCTCAA GATGGAAGAC CGACTGAAAC TGGTCAATGC CTCAGTGGCC
    TTTTATAAAA CTTCTGAGCA GGTCTGTAGT GTCCTGGAGA GCTTAGAGCA AGAATACCGA
    AGAGATGAGG ACTGGTGTGG TGGACGAGAT AAATTGGGGC CAGCAGCAGA GATCGACCAC
    GTCATTCCAC TCATCAGTAA ACATTTGGAA CAAAAGGAAG CCTTTCTTAA GGCCTGCACC
    CTGGCACGGC GGAATGCTGA GGTCTTTCTC AAGTACATCC ATAGGAACAA CGTCAGCATG
    CCCAGTGCTG CCAGCCACAC CCGAGGACCC GAACAGCAAG TGAAAGCCAT CCTGAGTGAG
    CTCTTACAGA GGGAGAACCG TGTCCTGCAC TTCTGGACAT TGAAGAAGCG GCGGTTAGAC
    CAGTGTCAGC AATACGTGGT ATTTGAGCGA AGCGCCAAGC AGGCACTAGA CTGGATCCAA
    GAAACAGGTG AATATTACCT CTCAACACAT ACCTCCACCG GGGAGACCAC AGAGGAGACT
    CAGGAACTGC TGAAAGAATA TGGGGAGTTC AGGGTGCCTG CCAAGCAAAC AAAGGAGAAG
    GTGAAGCTTC TGATTCAGCT GGCTGATAGC TTTGTGGAAA AAGGTCACAT TCATGCCACA
    GAGATCAGGA AATGGGTGAC CACAGTGGAC AAGCATTACA GAGACTTCTC ACTGAGGATG
    GGAAAATACC GGTACTCCCT GGAGAAGGCC CTAGGAGTCA ACACGGAGGA TAACAAGGAC
    CTGGAGCTGG ACATCATCCC AGCAAGCCTT TCAGATCGTG AGGTCAAGCT CCGGGATGCC
    AACCACGAGG TCAATGAAGA GAAGCGGAAA TCAGCCAGGA AAAAAGAATT CATTATGGCA
    GAATTGCTCC AGACAGAGAA GGCTTATGTG AGGGACTTAC ATGAGTGCTT AGAGACCTAC
    CTGTGGGAAA TGACCAGTGG TGTGGAGGAG ATCCCCCCTG GGATCCTTAA TAAGGAGCAT
    ATCATCTTTG GCAACATCCA AGAGATCTAC GATTTTCATA ACAACATCTT CCTCAAAGAA
    CTGGAGAAGT ATGAGCAGCT GCCTGAGGAT GTGGGACACT GCTTTGTTAC TTGGGCAGAC
    AAATTTCAGA TGTACGTCAC CTACTGTAAA AACAAGCCTG ATTCCAACCA GCTGATCCTG
    GAGCATGCAG GCACCTTCTT TGATGAGATA CAGCAGCGGC ATGGTCTGGC CAACTCCATT
    TCTTCCTACC TAATTAAGCC TGTCCAGAGG ATTACCAAGT ACCAGCTGCT CCTGAAGGAA
    CTTTTAACTT GCTGTGAAGA AGGAAAAGGG GAGCTCAAGG AAGGCCTGGA GGTGATGCTT
    AGTGTCCCAA AGAGAGCCAA CGATGCCATG CACGTCAGCA TGCTGGAAGG GTTCGACGAG
    AACCTGGACG TGCAGGGGGA GCTGATTTTA CAAGATGCCT TTCAAGTGTG GGACCCCAAG
    TCGCTGATCC GGAAAGGGCG GGAGCGACAC CTATTCCTCT TTGAGATCTC CTTGGTTTTC
    AGCAAGGAGA TCAAAGACTC TTCAGGACAC ACAAAATATG TTTACAAGAA CAAGCTACTG
    ACCTCAGAGC TGGGTGTGAC CGAACACGTA GAGGGCGATC CCTGCAAATT CGCCTTGTGG
    TCTGGGCGCA CCCCATCCTC AGACAATAAA ACAGTGCTGA AAGCCTCCAA CATAGAAACC
    AAGCAGGAGT GGATCAAGAA CATTCGAGAG GTGATTCAAG AAAGGATCAT TCACCTGAAA
    GGAGCTTTGA AGGAGCCAAT TCAGCTCCCC AAAACACCAG CCAAACAGAG GAACAACAGT
    AAGAGGGATG GAGTGGAGGA TGTTGACAGC CAGGGGGATG GGAGCAGCCA GCCAGACACC
    ATCTCCATTG CCTCTAGGAC CTCCCAGAAC ACAGCGGACA GTGACAAGGA TGGCAACCTT
    GCTCCTCGGT GGTACCTGGG ACCTGGAGAT CCTTTCTCCA CTTACGTTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847152.1
    CDS360..5330
    Translation

    Target ORF information:

    RefSeq Version XM_024991384.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGA AGACACACCA GAGCGCCCTG CAGGTTCAGC AGAAAGCTGA GGCGCTGCTC
    CAGGCTGGTC ACTATGATGC AGATGCCATC CGGGAATGTG CTGAGAAGGT GGCCCTCCAC
    TGGCAGCAAC TCATGCTCAA GATGGAAGAC CGACTGAAAC TGGTCAATGC CTCAGTGGCC
    TTTTATAAAA CTTCTGAGCA GGTCTGTAGT GTCCTGGAGA GCTTAGAGCA AGAATACCGA
    AGAGATGAGG ACTGGTGTGG TGGACGAGAT AAATTGGGGC CAGCAGCAGA GATCGACCAC
    GTCATTCCAC TCATCAGTAA ACATTTGGAA CAAAAGGAAG CCTTTCTTAA GGCCTGCACC
    CTGGCACGGC GGAATGCTGA GGTCTTTCTC AAGTACATCC ATAGGAACAA CGTCAGCATG
    CCCAGTGCTG CCAGCCACAC CCGAGGACCC GAACAGCAAG TGAAAGCCAT CCTGAGTGAG
    CTCTTACAGA GGGAGAACCG TGTCCTGCAC TTCTGGACAT TGAAGAAGCG GCGGTTAGAC
    CAGTGTCAGC AATACGTGGT ATTTGAGCGA AGCGCCAAGC AGGCACTAGA CTGGATCCAA
    GAAACAGGTG AATATTACCT CTCAACACAT ACCTCCACCG GGGAGACCAC AGAGGAGACT
    CAGGAACTGC TGAAAGAATA TGGGGAGTTC AGGGTGCCTG CCAAGCAAAC AAAGGAGAAG
    GTGAAGCTTC TGATTCAGCT GGCTGATAGC TTTGTGGAAA AAGGTCACAT TCATGCCACA
    GAGATCAGGA AATGGGTGAC CACAGTGGAC AAGCATTACA GAGACTTCTC ACTGAGGATG
    GGAAAATACC GGTACTCCCT GGAGAAGGCC CTAGGAGTCA ACACGGAGGA TAACAAGGAC
    CTGGAGCTGG ACATCATCCC AGCAAGCCTT TCAGATCGTG AGGTCAAGCT CCGGGATGCC
    AACCACGAGG TCAATGAAGA GAAGCGGAAA TCAGCCAGGA AAAAAGAATT CATTATGGCA
    GAATTGCTCC AGACAGAGAA GGCTTATGTG AGGGACTTAC ATGAGTGCTT AGAGACCTAC
    CTGTGGGAAA TGACCAGTGG TGTGGAGGAG ATCCCCCCTG GGATCCTTAA TAAGGAGCAT
    ATCATCTTTG GCAACATCCA AGAGATCTAC GATTTTCATA ACAACATCTT CCTCAAAGAA
    CTGGAGAAGT ATGAGCAGCT GCCTGAGGAT GTGGGACACT GCTTTGTTAC TTGGGCAGAC
    AAATTTCAGA TGTACGTCAC CTACTGTAAA AACAAGCCTG ATTCCAACCA GCTGATCCTG
    GAGCATGCAG GCACCTTCTT TGATGAGATA CAGCAGCGGC ATGGTCTGGC CAACTCCATT
    TCTTCCTACC TAATTAAGCC TGTCCAGAGG ATTACCAAGT ACCAGCTGCT CCTGAAGGAA
    CTTTTAACTT GCTGTGAAGA AGGAAAAGGG GAGCTCAAGG AAGGCCTGGA GGTGATGCTT
    AGTGTCCCAA AGAGAGCCAA CGATGCCATG CACGTCAGCA TGCTGGAAGG GTTCGACGAG
    AACCTGGACG TGCAGGGGGA GCTGATTTTA CAAGATGCCT TTCAAGTGTG GGACCCCAAG
    TCGCTGATCC GGAAAGGGCG GGAGCGACAC CTATTCCTCT TTGAGATCTC CTTGGTTTTC
    AGCAAGGAGA TCAAAGACTC TTCAGGACAC ACAAAATATG TTTACAAGAA CAAGCTACTG
    ACCTCAGAGC TGGGTGTGAC CGAACACGTA GAGGGCGATC CCTGCAAATT CGCCTTGTGG
    TCTGGGCGCA CCCCATCCTC AGACAATAAA ACAGTGCTGA AAGCCTCCAA CATAGAAACC
    AAGCAGGAGT GGATCAAGAA CATTCGAGAG GTGATTCAAG AAAGGATCAT TCACCTGAAA
    GGAGCTTTGA AGGAGCCAAT TCAGCTCCCC AAAACACCAG CCAAACAGAG GAACAACAGT
    AAGAGGGATG GAGTGGAGGA TGTTGACAGC CAGGGGGATG GGAGCAGCCA GCCAGACACC
    ATCTCCATTG CCTCTAGGAC CTCCCAGAAC ACAGCGGACA GTGACAAGGA TGGCAACCTT
    GCTCCTCGGT GGTACCTGGG ACCTGGAGAT CCTTTCTCCA CTTACGTTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218900
    Clone ID Related Accession (Same CDS sequence) XM_024991386.1
    Accession Version XM_024991386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGACC GCTTCTGGGA ACAGTGGTAT CTTTGGTATC TCCGCTTGCT CCGGCTGCTG 
    GATCGAGGCT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC CATCCTAAAG
    GAAAAAGTGG CCTTCGTGTC TGGGGGCCGT GATAAGCGAG GTGGACCCAT CCTGACCTTC
    CCTGCTCGCA GCAATCATGA CAGAATAAGA CAGGAAGACC TGCGGAAACT GGTGACCTAT
    CTGGCCAGCG TGCCAAGCGA GGACGTGTGC AAACGTGGCT TCACTGTCAT CATCGATATG
    CGGGGGTCCA AGTGGGACCT CATCAAGCCC CTCCTCAAGA CGCTGCAAGA AGCCTTTCCC
    GCTGAGATCC ATGTGGCCCT CATCATCAAA CCCGACAACT TCTGGCAGAA ACAGAAGACC
    AACTTTGGCA GCTCCAAGTT CATCTTTGAG ACAAGCATGG TGTCCGTGGA GGGCCTCACG
    AAGCTGGTGG ACCCCTCCCA GCTGACGGAG GAGTTCGATG GCTCCCTGGA CTACAACCAC
    GAGGAGTGGA TCGAGCTGCG GCTCTCCCTG GAGGAGTTCT TCAACAGCGC CGTACACCTG
    CTCTCACGCC TTGAAGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC CGTGGATGTG
    GAGGGCTCGA GGCGGCTAAT TGATGAACAC ACACAGCTGA AGAAGAAGGT GCTGAAGGCG
    CCTGTGGAGG AGCTGGACCG GGAGGGGCAG CGACTGCTGC AGTGCATCCG CTGCAGTGAC
    GGCTTCTCAG GGCGCAACTG CATCCCGGGC AGTGCCGACT TCCAGAGCTT GGTGCCCAAG
    ATCACCAGCC TGTTGGACAA GCTGCACTCC ACCCGGCAGC ACCTGCACCA GATGTGGCAT
    GTGCGCAAGC TCAAGCTAGA CCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAA
    AAGATGTTTG ACTGGATAAG CCACAACAAG GAGTTATTCC TCCAGAGCCA TACAGAGATT
    GGAGTCAGCT ACCAGCACGC CCTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC
    TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TGGCTTCCCG CCTCTCAGAG
    GCTGGCCACT ATGCTTCACA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG
    AAGAGTTTCG CAGCTGCCCT GGATGAACGC AGCACCATCC TCGCCATGTC TGCTGTGTTC
    CATCAGAAGG CTGAGCAGTT CTTGTCAGGA GTGGATGCCT GGTGCAAGAT GTGCAGTGAA
    GGTGGCCTGC CCTCTGAGAT GCAGGACCTG GAGCTGGCGA TCCACCACCA CCAGTCTTTG
    TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ACGGCAAAGC ACTGCTGGAT
    GTGCTGCAGC GGCCCCTGAG TCCCGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC
    TCCAAGGCGG TGCACCAGGT GCTGGACGTG GTGCACGAAG TGCTGCACCA CCAGCGGCGG
    CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTCCACC AACGCCTGCA GCTCTGCGTC
    TTCCAGCAGG ACGTCCAGCA GGTGTTAGAT TGGATTGAAA ACCACGGCGA GGCCTTTCTC
    AGCAAGCACA CAGGGGTCGG GAAGTCTCTG CACCGAGCTC GGGCCCTGCA GAAGAGGCAC
    GATGACTTTG AAGAGGTGGC TCAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCG
    GCAGAGCAGC TGGCTCAGAC AGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCTAGA
    CACCTGGAGG TGCGCATCCA AGACTTCGTG CGCAGGGTGG AGCAGCGGAA GCTTCTGCTG
    GACATGTCTG TCTCCTTCCA TACTCACACC AAAGAGCTAT GGACATGGAT GGAAGACCTT
    CAGAAGGAGA TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAACTGATC
    AAGCAGTTCC AGCAGCAGCA GACCGCCACT CTGGATGCCA CACTTAATGT CATCAAGGAG
    GGCGAAGACC TTATCCAGCA ACTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCAGCGAG
    GCCAGGGACT CAGCTGTGTC CAACAACAAG ACGCCCCATA GTAGCTCCAT CAGCCACATA
    GAGTCGGTGC TGCAGCAGCT GGATGACGCC CAGGTGCAAA TGGAGGAGCT GTTCCACGAA
    CGCAAGATCA AGCTGGACAT CTTCCTGCAG CTGCGCATCT TTGAGCAGTA CACCATTGAG
    GTGACAGCAG AGCTCGACGC TTGGAATGAG GATTTGCTTC GGCAGATGAA TGACTTCAAC
    ACGGAGGATC TAACACTGGC GGAACAGCGG CTGCAGCGCC ACACAGAGCG GAAGCTAGCC
    ATGAACAACA TGACCTTTGA GGTCATCCAG CAAGGCCAGG ATCTGCACCA GTACATCATG
    GAGGTCCAGG CCTCAGGGAT TGAGTTAATC TGTGAAAAAG ACATTGATCT GGCAGCCCAG
    GTACAAGAGC TATTAGAGTT TCTCCATGAG AAACAGCATG AGTTGGAGCT CAACGCAGAG
    CAGACCCACA AGCGACTTGA GCAGTGCCTC CAACTGCGGC ACCTCCAGGC CGAGGTCAAA
    CAGGTCCTAG GATGGATCCG CAATGGGGAG TCAATGCTCA ATGCCAGCCT GGTCAACGCC
    AGCTCTTTGT CTGAAGCAGA GCAGCTACAG CGGGAGCACG AGCAGTTCCA ACTAGCCATC
    GAGAAGACAC ACCAGAGCGC CCTGCAGGTT CAGCAGAAAG CTGAGGCGCT GCTCCAGGCT
    GGTCACTATG ATGCAGATGC CATCCGGGAA TGTGCTGAGA AGGTGGCCCT CCACTGGCAG
    CAACTCATGC TCAAGATGGA AGACCGACTG AAACTGGTCA ATGCCTCAGT GGCCTTTTAT
    AAAACTTCTG AGCAGGTCTG TAGTGTCCTG GAGAGCTTAG AGCAAGAATA CCGAAGAGAT
    GAGGACTGGT GTGGTGGACG AGATAAATTG GGGCCAGCAG CAGAGATCGA CCACGTCATT
    CCACTCATCA GTAAACATTT GGAACAAAAG GAAGCCTTTC TTAAGGCCTG CACCCTGGCA
    CGGCGGAATG CTGAGGTCTT TCTCAAGTAC ATCCATAGGA ACAACGTCAG CATGCCCAGT
    GCTGCCAGCC ACACCCGAGG ACCCGAACAG CAAGTGAAAG CCATCCTGAG TGAGCTCTTA
    CAGAGGGAGA ACCGTGTCCT GCACTTCTGG ACATTGAAGA AGCGGCGGTT AGACCAGTGT
    CAGCAATACG TGGTATTTGA GCGAAGCGCC AAGCAGGCAC TAGACTGGAT CCAAGAAACA
    GGTGAATATT ACCTCTCAAC ACATACCTCC ACCGGGGAGA CCACAGAGGA GACTCAGGAA
    CTGCTGAAAG AATATGGGGA GTTCAGGGTG CCTGCCAAGC AAACAAAGGA GAAGGTGAAG
    CTTCTGATTC AGCTGGCTGA TAGCTTTGTG GAAAAAGGTC ACATTCATGC CACAGAGATC
    AGGAAATGGG TGACCACAGT GGACAAGCAT TACAGAGACT TCTCACTGAG GATGGGAAAA
    TACCGGTACT CCCTGGAGAA GGCCCTAGGA GTCAACACGG AGGATAACAA GGACCTGGAG
    CTGGACATCA TCCCAGCAAG CCTTTCAGAT CGTGAGGTCA AGCTCCGGGA TGCCAACCAC
    GAGGTCAATG AAGAGAAGCG GAAATCAGCC AGGAAAAAAG AATTCATTAT GGCAGAATTG
    CTCCAGACAG AGAAGGCTTA TGTGAGGGAC TTACATGAGT GCTTAGAGAC CTACCTGTGG
    GAAATGACCA GTGGTGTGGA GGAGATCCCC CCTGGGATCC TTAATAAGGA GCATATCATC
    TTTGGCAACA TCCAAGAGAT CTACGATTTT CATAACAACA TCTTCCTCAA AGAACTGGAG
    AAGTATGAGC AGCTGCCTGA GGATGTGGGA CACTGCTTTG TTACTTGGGC AGACAAATTT
    CAGATGTACG TCACCTACTG TAAAAACAAG CCTGATTCCA ACCAGCTGAT CCTGGAGCAT
    GCAGGCACCT TCTTTGATGA GATACAGCAG CGGCATGGTC TGGCCAACTC CATTTCTTCC
    TACCTAATTA AGCCTGTCCA GAGGATTACC AAGTACCAGC TGCTCCTGAA GGAACTTTTA
    ACTTGCTGTG AAGAAGGAAA AGGGGAGCTC AAGGAAGGCC TGGAGGTGAT GCTTAGTGTC
    CCAAAGAGAG CCAACGATGC CATGCACGTC AGCATGCTGG AAGGGTTCGA CGAGAACCTG
    GACGTGCAGG GGGAGCTGAT TTTACAAGAT GCCTTTCAAG TGTGGGACCC CAAGTCGCTG
    ATCCGGAAAG GGCGGGAGCG ACACCTATTC CTCTTTGAGA TCTCCTTGGT TTTCAGCAAG
    GAGATCAAAG ACTCTTCAGG ACACACAAAA TATGTTTACA AGAACAAGCT ACTGACCTCA
    GAGCTGGGTG TGACCGAACA CGTAGAGGGC GATCCCTGCA AATTCGCCTT GTGGTCTGGG
    CGCACCCCAT CCTCAGACAA TAAAACAGTG CTGAAAGCCT CCAACATAGA AACCAAGCAG
    GAGTGGATCA AGAACATTCG AGAGGTGATT CAAGAAAGGA TCATTCACCT GAAAGGAGCT
    TTGAAGGAGC CAATTCAGCT CCCCAAAACA CCAGCCAAAC AGAGGAACAA CAGTAAGAGG
    GATGGAGTGG AGGATGTTGA CAGCCAGGGG GATGGGAGCA GCCAGCCAGA CACCATCTCC
    ATTGCCTCTA GGACCTCCCA GAACACAGCG GACAGTGACA AGGATGGCAA CCTTGCTCCT
    CGGTGGTACC TGGGACCTGG AGATCCTTTC TCCACTTACG TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847154.1
    CDS5083..10047
    Translation

    Target ORF information:

    RefSeq Version XM_024991386.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGACC GCTTCTGGGA ACAGTGGTAT CTTTGGTATC TCCGCTTGCT CCGGCTGCTG 
    GATCGAGGCT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC CATCCTAAAG
    GAAAAAGTGG CCTTCGTGTC TGGGGGCCGT GATAAGCGAG GTGGACCCAT CCTGACCTTC
    CCTGCTCGCA GCAATCATGA CAGAATAAGA CAGGAAGACC TGCGGAAACT GGTGACCTAT
    CTGGCCAGCG TGCCAAGCGA GGACGTGTGC AAACGTGGCT TCACTGTCAT CATCGATATG
    CGGGGGTCCA AGTGGGACCT CATCAAGCCC CTCCTCAAGA CGCTGCAAGA AGCCTTTCCC
    GCTGAGATCC ATGTGGCCCT CATCATCAAA CCCGACAACT TCTGGCAGAA ACAGAAGACC
    AACTTTGGCA GCTCCAAGTT CATCTTTGAG ACAAGCATGG TGTCCGTGGA GGGCCTCACG
    AAGCTGGTGG ACCCCTCCCA GCTGACGGAG GAGTTCGATG GCTCCCTGGA CTACAACCAC
    GAGGAGTGGA TCGAGCTGCG GCTCTCCCTG GAGGAGTTCT TCAACAGCGC CGTACACCTG
    CTCTCACGCC TTGAAGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC CGTGGATGTG
    GAGGGCTCGA GGCGGCTAAT TGATGAACAC ACACAGCTGA AGAAGAAGGT GCTGAAGGCG
    CCTGTGGAGG AGCTGGACCG GGAGGGGCAG CGACTGCTGC AGTGCATCCG CTGCAGTGAC
    GGCTTCTCAG GGCGCAACTG CATCCCGGGC AGTGCCGACT TCCAGAGCTT GGTGCCCAAG
    ATCACCAGCC TGTTGGACAA GCTGCACTCC ACCCGGCAGC ACCTGCACCA GATGTGGCAT
    GTGCGCAAGC TCAAGCTAGA CCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAA
    AAGATGTTTG ACTGGATAAG CCACAACAAG GAGTTATTCC TCCAGAGCCA TACAGAGATT
    GGAGTCAGCT ACCAGCACGC CCTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC
    TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TGGCTTCCCG CCTCTCAGAG
    GCTGGCCACT ATGCTTCACA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG
    AAGAGTTTCG CAGCTGCCCT GGATGAACGC AGCACCATCC TCGCCATGTC TGCTGTGTTC
    CATCAGAAGG CTGAGCAGTT CTTGTCAGGA GTGGATGCCT GGTGCAAGAT GTGCAGTGAA
    GGTGGCCTGC CCTCTGAGAT GCAGGACCTG GAGCTGGCGA TCCACCACCA CCAGTCTTTG
    TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ACGGCAAAGC ACTGCTGGAT
    GTGCTGCAGC GGCCCCTGAG TCCCGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC
    TCCAAGGCGG TGCACCAGGT GCTGGACGTG GTGCACGAAG TGCTGCACCA CCAGCGGCGG
    CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTCCACC AACGCCTGCA GCTCTGCGTC
    TTCCAGCAGG ACGTCCAGCA GGTGTTAGAT TGGATTGAAA ACCACGGCGA GGCCTTTCTC
    AGCAAGCACA CAGGGGTCGG GAAGTCTCTG CACCGAGCTC GGGCCCTGCA GAAGAGGCAC
    GATGACTTTG AAGAGGTGGC TCAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCG
    GCAGAGCAGC TGGCTCAGAC AGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCTAGA
    CACCTGGAGG TGCGCATCCA AGACTTCGTG CGCAGGGTGG AGCAGCGGAA GCTTCTGCTG
    GACATGTCTG TCTCCTTCCA TACTCACACC AAAGAGCTAT GGACATGGAT GGAAGACCTT
    CAGAAGGAGA TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAACTGATC
    AAGCAGTTCC AGCAGCAGCA GACCGCCACT CTGGATGCCA CACTTAATGT CATCAAGGAG
    GGCGAAGACC TTATCCAGCA ACTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCAGCGAG
    GCCAGGGACT CAGCTGTGTC CAACAACAAG ACGCCCCATA GTAGCTCCAT CAGCCACATA
    GAGTCGGTGC TGCAGCAGCT GGATGACGCC CAGGTGCAAA TGGAGGAGCT GTTCCACGAA
    CGCAAGATCA AGCTGGACAT CTTCCTGCAG CTGCGCATCT TTGAGCAGTA CACCATTGAG
    GTGACAGCAG AGCTCGACGC TTGGAATGAG GATTTGCTTC GGCAGATGAA TGACTTCAAC
    ACGGAGGATC TAACACTGGC GGAACAGCGG CTGCAGCGCC ACACAGAGCG GAAGCTAGCC
    ATGAACAACA TGACCTTTGA GGTCATCCAG CAAGGCCAGG ATCTGCACCA GTACATCATG
    GAGGTCCAGG CCTCAGGGAT TGAGTTAATC TGTGAAAAAG ACATTGATCT GGCAGCCCAG
    GTACAAGAGC TATTAGAGTT TCTCCATGAG AAACAGCATG AGTTGGAGCT CAACGCAGAG
    CAGACCCACA AGCGACTTGA GCAGTGCCTC CAACTGCGGC ACCTCCAGGC CGAGGTCAAA
    CAGGTCCTAG GATGGATCCG CAATGGGGAG TCAATGCTCA ATGCCAGCCT GGTCAACGCC
    AGCTCTTTGT CTGAAGCAGA GCAGCTACAG CGGGAGCACG AGCAGTTCCA ACTAGCCATC
    GAGAAGACAC ACCAGAGCGC CCTGCAGGTT CAGCAGAAAG CTGAGGCGCT GCTCCAGGCT
    GGTCACTATG ATGCAGATGC CATCCGGGAA TGTGCTGAGA AGGTGGCCCT CCACTGGCAG
    CAACTCATGC TCAAGATGGA AGACCGACTG AAACTGGTCA ATGCCTCAGT GGCCTTTTAT
    AAAACTTCTG AGCAGGTCTG TAGTGTCCTG GAGAGCTTAG AGCAAGAATA CCGAAGAGAT
    GAGGACTGGT GTGGTGGACG AGATAAATTG GGGCCAGCAG CAGAGATCGA CCACGTCATT
    CCACTCATCA GTAAACATTT GGAACAAAAG GAAGCCTTTC TTAAGGCCTG CACCCTGGCA
    CGGCGGAATG CTGAGGTCTT TCTCAAGTAC ATCCATAGGA ACAACGTCAG CATGCCCAGT
    GCTGCCAGCC ACACCCGAGG ACCCGAACAG CAAGTGAAAG CCATCCTGAG TGAGCTCTTA
    CAGAGGGAGA ACCGTGTCCT GCACTTCTGG ACATTGAAGA AGCGGCGGTT AGACCAGTGT
    CAGCAATACG TGGTATTTGA GCGAAGCGCC AAGCAGGCAC TAGACTGGAT CCAAGAAACA
    GGTGAATATT ACCTCTCAAC ACATACCTCC ACCGGGGAGA CCACAGAGGA GACTCAGGAA
    CTGCTGAAAG AATATGGGGA GTTCAGGGTG CCTGCCAAGC AAACAAAGGA GAAGGTGAAG
    CTTCTGATTC AGCTGGCTGA TAGCTTTGTG GAAAAAGGTC ACATTCATGC CACAGAGATC
    AGGAAATGGG TGACCACAGT GGACAAGCAT TACAGAGACT TCTCACTGAG GATGGGAAAA
    TACCGGTACT CCCTGGAGAA GGCCCTAGGA GTCAACACGG AGGATAACAA GGACCTGGAG
    CTGGACATCA TCCCAGCAAG CCTTTCAGAT CGTGAGGTCA AGCTCCGGGA TGCCAACCAC
    GAGGTCAATG AAGAGAAGCG GAAATCAGCC AGGAAAAAAG AATTCATTAT GGCAGAATTG
    CTCCAGACAG AGAAGGCTTA TGTGAGGGAC TTACATGAGT GCTTAGAGAC CTACCTGTGG
    GAAATGACCA GTGGTGTGGA GGAGATCCCC CCTGGGATCC TTAATAAGGA GCATATCATC
    TTTGGCAACA TCCAAGAGAT CTACGATTTT CATAACAACA TCTTCCTCAA AGAACTGGAG
    AAGTATGAGC AGCTGCCTGA GGATGTGGGA CACTGCTTTG TTACTTGGGC AGACAAATTT
    CAGATGTACG TCACCTACTG TAAAAACAAG CCTGATTCCA ACCAGCTGAT CCTGGAGCAT
    GCAGGCACCT TCTTTGATGA GATACAGCAG CGGCATGGTC TGGCCAACTC CATTTCTTCC
    TACCTAATTA AGCCTGTCCA GAGGATTACC AAGTACCAGC TGCTCCTGAA GGAACTTTTA
    ACTTGCTGTG AAGAAGGAAA AGGGGAGCTC AAGGAAGGCC TGGAGGTGAT GCTTAGTGTC
    CCAAAGAGAG CCAACGATGC CATGCACGTC AGCATGCTGG AAGGGTTCGA CGAGAACCTG
    GACGTGCAGG GGGAGCTGAT TTTACAAGAT GCCTTTCAAG TGTGGGACCC CAAGTCGCTG
    ATCCGGAAAG GGCGGGAGCG ACACCTATTC CTCTTTGAGA TCTCCTTGGT TTTCAGCAAG
    GAGATCAAAG ACTCTTCAGG ACACACAAAA TATGTTTACA AGAACAAGCT ACTGACCTCA
    GAGCTGGGTG TGACCGAACA CGTAGAGGGC GATCCCTGCA AATTCGCCTT GTGGTCTGGG
    CGCACCCCAT CCTCAGACAA TAAAACAGTG CTGAAAGCCT CCAACATAGA AACCAAGCAG
    GAGTGGATCA AGAACATTCG AGAGGTGATT CAAGAAAGGA TCATTCACCT GAAAGGAGCT
    TTGAAGGAGC CAATTCAGCT CCCCAAAACA CCAGCCAAAC AGAGGAACAA CAGTAAGAGG
    GATGGAGTGG AGGATGTTGA CAGCCAGGGG GATGGGAGCA GCCAGCCAGA CACCATCTCC
    ATTGCCTCTA GGACCTCCCA GAACACAGCG GACAGTGACA AGGATGGCAA CCTTGCTCCT
    CGGTGGTACC TGGGACCTGG AGATCCTTTC TCCACTTACG TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218901
    Clone ID Related Accession (Same CDS sequence) XM_024991394.1
    Accession Version XM_024991394.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGGACTCAG CTGTGTCCAA CAACAAGACG
    CCCCATAGTA GCTCCATCAG CCACATAGAG TCGGTGCTGC AGCAGCTGGA TGACGCCCAG
    GTGCAAATGG AGGAGCTGTT CCACGAACGC AAGATCAAGC TGGACATCTT CCTGCAGCTG
    CGCATCTTTG AGCAGTACAC CATTGAGGTG ACAGCAGAGC TCGACGCTTG GAATGAGGAT
    TTGCTTCGGC AGATGAATGA CTTCAACACG GAGGATCTAA CACTGGCGGA ACAGCGGCTG
    CAGCGCCACA CAGAGCGGAA GCTAGCCATG AACAACATGA CCTTTGAGGT CATCCAGCAA
    GGCCAGGATC TGCACCAGTA CATCATGGAG GTCCAGGCCT CAGGGATTGA GTTAATCTGT
    GAAAAAGACA TTGATCTGGC AGCCCAGGTA CAAGAGCTAT TAGAGTTTCT CCATGAGAAA
    CAGCATGAGT TGGAGCTCAA CGCAGAGCAG ACCCACAAGC GACTTGAGCA GTGCCTCCAA
    CTGCGGCACC TCCAGGCCGA GGTCAAACAG GTCCTAGGAT GGATCCGCAA TGGGGAGTCA
    ATGCTCAATG CCAGCCTGGT CAACGCCAGC TCTTTGTCTG AAGCAGAGCA GCTACAGCGG
    GAGCACGAGC AGTTCCAACT AGCCATCGAG TCCCTCTTTC ATGCCACTTC CTTGCAGAAG
    ACACACCAGA GCGCCCTGCA GGTTCAGCAG AAAGCTGAGG CGCTGCTCCA GGCTGGTCAC
    TATGATGCAG ATGCCATCCG GGAATGTGCT GAGAAGGTGG CCCTCCACTG GCAGCAACTC
    ATGCTCAAGA TGGAAGACCG ACTGAAACTG GTCAATGCCT CAGTGGCCTT TTATAAAACT
    TCTGAGCAGG TCTGTAGTGT CCTGGAGAGC TTAGAGCAAG AATACCGAAG AGATGAGGAC
    TGGTGTGGTG GACGAGATAA ATTGGGGCCA GCAGCAGAGA TCGACCACGT CATTCCACTC
    ATCAGTAAAC ATTTGGAACA AAAGGAAGCC TTTCTTAAGG CCTGCACCCT GGCACGGCGG
    AATGCTGAGG TCTTTCTCAA GTACATCCAT AGGAACAACG TCAGCATGCC CAGTGCTGCC
    AGCCACACCC GAGGACCCGA ACAGCAAGTG AAAGCCATCC TGAGTGAGCT CTTACAGAGG
    GAGAACCGTG TCCTGCACTT CTGGACATTG AAGAAGCGGC GGTTAGACCA GTGTCAGCAA
    TACGTGGTAT TTGAGCGAAG CGCCAAGCAG GCACTAGACT GGATCCAAGA AACAGGTGAA
    TATTACCTCT CAACACATAC CTCCACCGGG GAGACCACAG AGGAGACTCA GGAACTGCTG
    AAAGAATATG GGGAGTTCAG GGTGCCTGCC AAGCAAACAA AGGAGAAGGT GAAGCTTCTG
    ATTCAGCTGG CTGATAGCTT TGTGGAAAAA GGTCACATTC ATGCCACAGA GATCAGGAAA
    TGGGTGACCA CAGTGGACAA GCATTACAGA GACTTCTCAC TGAGGATGGG AAAATACCGG
    TACTCCCTGG AGAAGGCCCT AGGAGTCAAC ACGGAGGATA ACAAGGACCT GGAGCTGGAC
    ATCATCCCAG CAAGCCTTTC AGATCGTGAG GTCAAGCTCC GGGATGCCAA CCACGAGGTC
    AATGAAGAGA AGCGGAAATC AGCCAGGAAA AAAGAATTCA TTATGGCAGA ATTGCTCCAG
    ACAGAGAAGG CTTATGTGAG GGACTTACAT GAGTGCTTAG AGACCTACCT GTGGGAAATG
    ACCAGTGGTG TGGAGGAGAT CCCCCCTGGG ATCCTTAATA AGGAGCATAT CATCTTTGGC
    AACATCCAAG AGATCTACGA TTTTCATAAC AACATCTTCC TCAAAGAACT GGAGAAGTAT
    GAGCAGCTGC CTGAGGATGT GGGACACTGC TTTGTTACTT GGGCAGACAA ATTTCAGATG
    TACGTCACCT ACTGTAAAAA CAAGCCTGAT TCCAACCAGC TGATCCTGGA GCATGCAGGC
    ACCTTCTTTG ATGAGATACA GCAGCGGCAT GGTCTGGCCA ACTCCATTTC TTCCTACCTA
    ATTAAGCCTG TCCAGAGGAT TACCAAGTAC CAGCTGCTCC TGAAGGAACT TTTAACTTGC
    TGTGAAGAAG GAAAAGGGGA GCTCAAGGAA GGCCTGGAGG TGATGCTTAG TGTCCCAAAG
    AGAGCCAACG ATGCCATGCA CGTCAGCATG CTGGAAGGGT TCGACGAGAA CCTGGACGTG
    CAGGGGGAGC TGATTTTACA AGATGCCTTT CAAGTGTGGG ACCCCAAGTC GCTGATCCGG
    AAAGGGCGGG AGCGACACCT ATTCCTCTTT GAGATCTCCT TGGTTTTCAG CAAGGAGATC
    AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGCTACTGAC CTCAGAGCTG
    GGTGTGACCG AACACGTAGA GGGCGATCCC TGCAAATTCG CCTTGTGGTC TGGGCGCACC
    CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCCTCCAACA TAGAAACCAA GCAGGAGTGG
    ATCAAGAACA TTCGAGAGGT GATTCAAGAA AGGATCATTC ACCTGAAAGG AGCTTTGAAG
    GAGCCAATTC AGCTCCCCAA AACACCAGCC AAACAGAGGA ACAACAGTAA GAGGGATGGA
    GTGGAGGATG TTGACAGCCA GGGGGATGGG AGCAGCCAGC CAGACACCAT CTCCATTGCC
    TCTAGGACCT CCCAGAACAC AGCGGACAGT GACAAGGATG GCAACCTTGC TCCTCGGTGG
    TACCTGGGAC CTGGAGATCC TTTCTCCACT TACGTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847162.1
    CDS360..5318
    Translation

    Target ORF information:

    RefSeq Version XM_024991394.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991394.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGGACTCAG CTGTGTCCAA CAACAAGACG
    CCCCATAGTA GCTCCATCAG CCACATAGAG TCGGTGCTGC AGCAGCTGGA TGACGCCCAG
    GTGCAAATGG AGGAGCTGTT CCACGAACGC AAGATCAAGC TGGACATCTT CCTGCAGCTG
    CGCATCTTTG AGCAGTACAC CATTGAGGTG ACAGCAGAGC TCGACGCTTG GAATGAGGAT
    TTGCTTCGGC AGATGAATGA CTTCAACACG GAGGATCTAA CACTGGCGGA ACAGCGGCTG
    CAGCGCCACA CAGAGCGGAA GCTAGCCATG AACAACATGA CCTTTGAGGT CATCCAGCAA
    GGCCAGGATC TGCACCAGTA CATCATGGAG GTCCAGGCCT CAGGGATTGA GTTAATCTGT
    GAAAAAGACA TTGATCTGGC AGCCCAGGTA CAAGAGCTAT TAGAGTTTCT CCATGAGAAA
    CAGCATGAGT TGGAGCTCAA CGCAGAGCAG ACCCACAAGC GACTTGAGCA GTGCCTCCAA
    CTGCGGCACC TCCAGGCCGA GGTCAAACAG GTCCTAGGAT GGATCCGCAA TGGGGAGTCA
    ATGCTCAATG CCAGCCTGGT CAACGCCAGC TCTTTGTCTG AAGCAGAGCA GCTACAGCGG
    GAGCACGAGC AGTTCCAACT AGCCATCGAG TCCCTCTTTC ATGCCACTTC CTTGCAGAAG
    ACACACCAGA GCGCCCTGCA GGTTCAGCAG AAAGCTGAGG CGCTGCTCCA GGCTGGTCAC
    TATGATGCAG ATGCCATCCG GGAATGTGCT GAGAAGGTGG CCCTCCACTG GCAGCAACTC
    ATGCTCAAGA TGGAAGACCG ACTGAAACTG GTCAATGCCT CAGTGGCCTT TTATAAAACT
    TCTGAGCAGG TCTGTAGTGT CCTGGAGAGC TTAGAGCAAG AATACCGAAG AGATGAGGAC
    TGGTGTGGTG GACGAGATAA ATTGGGGCCA GCAGCAGAGA TCGACCACGT CATTCCACTC
    ATCAGTAAAC ATTTGGAACA AAAGGAAGCC TTTCTTAAGG CCTGCACCCT GGCACGGCGG
    AATGCTGAGG TCTTTCTCAA GTACATCCAT AGGAACAACG TCAGCATGCC CAGTGCTGCC
    AGCCACACCC GAGGACCCGA ACAGCAAGTG AAAGCCATCC TGAGTGAGCT CTTACAGAGG
    GAGAACCGTG TCCTGCACTT CTGGACATTG AAGAAGCGGC GGTTAGACCA GTGTCAGCAA
    TACGTGGTAT TTGAGCGAAG CGCCAAGCAG GCACTAGACT GGATCCAAGA AACAGGTGAA
    TATTACCTCT CAACACATAC CTCCACCGGG GAGACCACAG AGGAGACTCA GGAACTGCTG
    AAAGAATATG GGGAGTTCAG GGTGCCTGCC AAGCAAACAA AGGAGAAGGT GAAGCTTCTG
    ATTCAGCTGG CTGATAGCTT TGTGGAAAAA GGTCACATTC ATGCCACAGA GATCAGGAAA
    TGGGTGACCA CAGTGGACAA GCATTACAGA GACTTCTCAC TGAGGATGGG AAAATACCGG
    TACTCCCTGG AGAAGGCCCT AGGAGTCAAC ACGGAGGATA ACAAGGACCT GGAGCTGGAC
    ATCATCCCAG CAAGCCTTTC AGATCGTGAG GTCAAGCTCC GGGATGCCAA CCACGAGGTC
    AATGAAGAGA AGCGGAAATC AGCCAGGAAA AAAGAATTCA TTATGGCAGA ATTGCTCCAG
    ACAGAGAAGG CTTATGTGAG GGACTTACAT GAGTGCTTAG AGACCTACCT GTGGGAAATG
    ACCAGTGGTG TGGAGGAGAT CCCCCCTGGG ATCCTTAATA AGGAGCATAT CATCTTTGGC
    AACATCCAAG AGATCTACGA TTTTCATAAC AACATCTTCC TCAAAGAACT GGAGAAGTAT
    GAGCAGCTGC CTGAGGATGT GGGACACTGC TTTGTTACTT GGGCAGACAA ATTTCAGATG
    TACGTCACCT ACTGTAAAAA CAAGCCTGAT TCCAACCAGC TGATCCTGGA GCATGCAGGC
    ACCTTCTTTG ATGAGATACA GCAGCGGCAT GGTCTGGCCA ACTCCATTTC TTCCTACCTA
    ATTAAGCCTG TCCAGAGGAT TACCAAGTAC CAGCTGCTCC TGAAGGAACT TTTAACTTGC
    TGTGAAGAAG GAAAAGGGGA GCTCAAGGAA GGCCTGGAGG TGATGCTTAG TGTCCCAAAG
    AGAGCCAACG ATGCCATGCA CGTCAGCATG CTGGAAGGGT TCGACGAGAA CCTGGACGTG
    CAGGGGGAGC TGATTTTACA AGATGCCTTT CAAGTGTGGG ACCCCAAGTC GCTGATCCGG
    AAAGGGCGGG AGCGACACCT ATTCCTCTTT GAGATCTCCT TGGTTTTCAG CAAGGAGATC
    AAAGACTCTT CAGGACACAC AAAATATGTT TACAAGAACA AGCTACTGAC CTCAGAGCTG
    GGTGTGACCG AACACGTAGA GGGCGATCCC TGCAAATTCG CCTTGTGGTC TGGGCGCACC
    CCATCCTCAG ACAATAAAAC AGTGCTGAAA GCCTCCAACA TAGAAACCAA GCAGGAGTGG
    ATCAAGAACA TTCGAGAGGT GATTCAAGAA AGGATCATTC ACCTGAAAGG AGCTTTGAAG
    GAGCCAATTC AGCTCCCCAA AACACCAGCC AAACAGAGGA ACAACAGTAA GAGGGATGGA
    GTGGAGGATG TTGACAGCCA GGGGGATGGG AGCAGCCAGC CAGACACCAT CTCCATTGCC
    TCTAGGACCT CCCAGAACAC AGCGGACAGT GACAAGGATG GCAACCTTGC TCCTCGGTGG
    TACCTGGGAC CTGGAGATCC TTTCTCCACT TACGTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218902
    Clone ID Related Accession (Same CDS sequence) XM_024991396.1
    Accession Version XM_024991396.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGGACTCAG CTGTGTCCAA CAACAAGACG
    CCCCATAGTA GCTCCATCAG CCACATAGAG TCGGTGCTGC AGCAGCTGGA TGACGCCCAG
    GTGCAAATGG AGGAGCTGTT CCACGAACGC AAGATCAAGC TGGACATCTT CCTGCAGCTG
    CGCATCTTTG AGCAGTACAC CATTGAGGTG ACAGCAGAGC TCGACGCTTG GAATGAGGAT
    TTGCTTCGGC AGATGAATGA CTTCAACACG GAGGATCTAA CACTGGCGGA ACAGCGGCTG
    CAGCGCCACA CAGAGCGGAA GCTAGCCATG AACAACATGA CCTTTGAGGT CATCCAGCAA
    GGCCAGGATC TGCACCAGTA CATCATGGAG GTCCAGGCCT CAGGGATTGA GTTAATCTGT
    GAAAAAGACA TTGATCTGGC AGCCCAGGTA CAAGAGCTAT TAGAGTTTCT CCATGAGAAA
    CAGCATGAGT TGGAGCTCAA CGCAGAGCAG ACCCACAAGC GACTTGAGCA GTGCCTCCAA
    CTGCGGCACC TCCAGGCCGA GGTCAAACAG GTCCTAGGAT GGATCCGCAA TGGGGAGTCA
    ATGCTCAATG CCAGCCTGGT CAACGCCAGC TCTTTGTCTG AAGCAGAGCA GCTACAGCGG
    GAGCACGAGC AGTTCCAACT AGCCATCGAG AAGACACACC AGAGCGCCCT GCAGGTTCAG
    CAGAAAGCTG AGGCGCTGCT CCAGGCTGGT CACTATGATG CAGATGCCAT CCGGGAATGT
    GCTGAGAAGG TGGCCCTCCA CTGGCAGCAA CTCATGCTCA AGATGGAAGA CCGACTGAAA
    CTGGTCAATG CCTCAGTGGC CTTTTATAAA ACTTCTGAGC AGGTCTGTAG TGTCCTGGAG
    AGCTTAGAGC AAGAATACCG AAGAGATGAG GACTGGTGTG GTGGACGAGA TAAATTGGGG
    CCAGCAGCAG AGATCGACCA CGTCATTCCA CTCATCAGTA AACATTTGGA ACAAAAGGAA
    GCCTTTCTTA AGGCCTGCAC CCTGGCACGG CGGAATGCTG AGGTCTTTCT CAAGTACATC
    CATAGGAACA ACGTCAGCAT GCCCAGTGCT GCCAGCCACA CCCGAGGACC CGAACAGCAA
    GTGAAAGCCA TCCTGAGTGA GCTCTTACAG AGGGAGAACC GTGTCCTGCA CTTCTGGACA
    TTGAAGAAGC GGCGGTTAGA CCAGTGTCAG CAATACGTGG TATTTGAGCG AAGCGCCAAG
    CAGGCACTAG ACTGGATCCA AGAAACAGGT GAATATTACC TCTCAACACA TACCTCCACC
    GGGGAGACCA CAGAGGAGAC TCAGGAACTG CTGAAAGAAT ATGGGGAGTT CAGGGTGCCT
    GCCAAGCAAA CAAAGGAGAA GGTGAAGCTT CTGATTCAGC TGGCTGATAG CTTTGTGGAA
    AAAGGTCACA TTCATGCCAC AGAGATCAGG AAATGGGTGA CCACAGTGGA CAAGCATTAC
    AGAGACTTCT CACTGAGGAT GGGAAAATAC CGGTACTCCC TGGAGAAGGC CCTAGGAGTC
    AACACGGAGG ATAACAAGGA CCTGGAGCTG GACATCATCC CAGCAAGCCT TTCAGATCGT
    GAGGTCAAGC TCCGGGATGC CAACCACGAG GTCAATGAAG AGAAGCGGAA ATCAGCCAGG
    AAAAAAGAAT TCATTATGGC AGAATTGCTC CAGACAGAGA AGGCTTATGT GAGGGACTTA
    CATGAGTGCT TAGAGACCTA CCTGTGGGAA ATGACCAGTG GTGTGGAGGA GATCCCCCCT
    GGGATCCTTA ATAAGGAGCA TATCATCTTT GGCAACATCC AAGAGATCTA CGATTTTCAT
    AACAACATCT TCCTCAAAGA ACTGGAGAAG TATGAGCAGC TGCCTGAGGA TGTGGGACAC
    TGCTTTGTTA CTTGGGCAGA CAAATTTCAG ATGTACGTCA CCTACTGTAA AAACAAGCCT
    GATTCCAACC AGCTGATCCT GGAGCATGCA GGCACCTTCT TTGATGAGAT ACAGCAGCGG
    CATGGTCTGG CCAACTCCAT TTCTTCCTAC CTAATTAAGC CTGTCCAGAG GATTACCAAG
    TACCAGCTGC TCCTGAAGGA ACTTTTAACT TGCTGTGAAG AAGGAAAAGG GGAGCTCAAG
    GAAGGCCTGG AGGTGATGCT TAGTGTCCCA AAGAGAGCCA ACGATGCCAT GCACGTCAGC
    ATGCTGGAAG GGTTCGACGA GAACCTGGAC GTGCAGGGGG AGCTGATTTT ACAAGATGCC
    TTTCAAGTGT GGGACCCCAA GTCGCTGATC CGGAAAGGGC GGGAGCGACA CCTATTCCTC
    TTTGAGATCT CCTTGGTTTT CAGCAAGGAG ATCAAAGACT CTTCAGGACA CACAAAATAT
    GTTTACAAGA ACAAGCTACT GACCTCAGAG CTGGGTGTGA CCGAACACGT AGAGGGCGAT
    CCCTGCAAAT TCGCCTTGTG GTCTGGGCGC ACCCCATCCT CAGACAATAA AACAGTGCTG
    AAAGCCTCCA ACATAGAAAC CAAGCAGGAG TGGATCAAGA ACATTCGAGA GGTGATTCAA
    GAAAGGATCA TTCACCTGAA AGGAGCTTTG AAGGAGCCAA TTCAGCTCCC CAAAACACCA
    GCCAAACAGA GGAACAACAG TAAGAGGGAT GGAGTGGAGG ATGTTGACAG CCAGGGGGAT
    GGGAGCAGCC AGCCAGACAC CATCTCCATT GCCTCTAGGA CCTCCCAGAA CACAGCGGAC
    AGTGACAAGG ATGGCAACCT TGCTCCTCGG TGGTACCTGG GACCTGGAGA TCCTTTCTCC
    ACTTACGTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847164.1
    CDS360..5291
    Translation

    Target ORF information:

    RefSeq Version XM_024991396.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991396.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGGACTCAG CTGTGTCCAA CAACAAGACG
    CCCCATAGTA GCTCCATCAG CCACATAGAG TCGGTGCTGC AGCAGCTGGA TGACGCCCAG
    GTGCAAATGG AGGAGCTGTT CCACGAACGC AAGATCAAGC TGGACATCTT CCTGCAGCTG
    CGCATCTTTG AGCAGTACAC CATTGAGGTG ACAGCAGAGC TCGACGCTTG GAATGAGGAT
    TTGCTTCGGC AGATGAATGA CTTCAACACG GAGGATCTAA CACTGGCGGA ACAGCGGCTG
    CAGCGCCACA CAGAGCGGAA GCTAGCCATG AACAACATGA CCTTTGAGGT CATCCAGCAA
    GGCCAGGATC TGCACCAGTA CATCATGGAG GTCCAGGCCT CAGGGATTGA GTTAATCTGT
    GAAAAAGACA TTGATCTGGC AGCCCAGGTA CAAGAGCTAT TAGAGTTTCT CCATGAGAAA
    CAGCATGAGT TGGAGCTCAA CGCAGAGCAG ACCCACAAGC GACTTGAGCA GTGCCTCCAA
    CTGCGGCACC TCCAGGCCGA GGTCAAACAG GTCCTAGGAT GGATCCGCAA TGGGGAGTCA
    ATGCTCAATG CCAGCCTGGT CAACGCCAGC TCTTTGTCTG AAGCAGAGCA GCTACAGCGG
    GAGCACGAGC AGTTCCAACT AGCCATCGAG AAGACACACC AGAGCGCCCT GCAGGTTCAG
    CAGAAAGCTG AGGCGCTGCT CCAGGCTGGT CACTATGATG CAGATGCCAT CCGGGAATGT
    GCTGAGAAGG TGGCCCTCCA CTGGCAGCAA CTCATGCTCA AGATGGAAGA CCGACTGAAA
    CTGGTCAATG CCTCAGTGGC CTTTTATAAA ACTTCTGAGC AGGTCTGTAG TGTCCTGGAG
    AGCTTAGAGC AAGAATACCG AAGAGATGAG GACTGGTGTG GTGGACGAGA TAAATTGGGG
    CCAGCAGCAG AGATCGACCA CGTCATTCCA CTCATCAGTA AACATTTGGA ACAAAAGGAA
    GCCTTTCTTA AGGCCTGCAC CCTGGCACGG CGGAATGCTG AGGTCTTTCT CAAGTACATC
    CATAGGAACA ACGTCAGCAT GCCCAGTGCT GCCAGCCACA CCCGAGGACC CGAACAGCAA
    GTGAAAGCCA TCCTGAGTGA GCTCTTACAG AGGGAGAACC GTGTCCTGCA CTTCTGGACA
    TTGAAGAAGC GGCGGTTAGA CCAGTGTCAG CAATACGTGG TATTTGAGCG AAGCGCCAAG
    CAGGCACTAG ACTGGATCCA AGAAACAGGT GAATATTACC TCTCAACACA TACCTCCACC
    GGGGAGACCA CAGAGGAGAC TCAGGAACTG CTGAAAGAAT ATGGGGAGTT CAGGGTGCCT
    GCCAAGCAAA CAAAGGAGAA GGTGAAGCTT CTGATTCAGC TGGCTGATAG CTTTGTGGAA
    AAAGGTCACA TTCATGCCAC AGAGATCAGG AAATGGGTGA CCACAGTGGA CAAGCATTAC
    AGAGACTTCT CACTGAGGAT GGGAAAATAC CGGTACTCCC TGGAGAAGGC CCTAGGAGTC
    AACACGGAGG ATAACAAGGA CCTGGAGCTG GACATCATCC CAGCAAGCCT TTCAGATCGT
    GAGGTCAAGC TCCGGGATGC CAACCACGAG GTCAATGAAG AGAAGCGGAA ATCAGCCAGG
    AAAAAAGAAT TCATTATGGC AGAATTGCTC CAGACAGAGA AGGCTTATGT GAGGGACTTA
    CATGAGTGCT TAGAGACCTA CCTGTGGGAA ATGACCAGTG GTGTGGAGGA GATCCCCCCT
    GGGATCCTTA ATAAGGAGCA TATCATCTTT GGCAACATCC AAGAGATCTA CGATTTTCAT
    AACAACATCT TCCTCAAAGA ACTGGAGAAG TATGAGCAGC TGCCTGAGGA TGTGGGACAC
    TGCTTTGTTA CTTGGGCAGA CAAATTTCAG ATGTACGTCA CCTACTGTAA AAACAAGCCT
    GATTCCAACC AGCTGATCCT GGAGCATGCA GGCACCTTCT TTGATGAGAT ACAGCAGCGG
    CATGGTCTGG CCAACTCCAT TTCTTCCTAC CTAATTAAGC CTGTCCAGAG GATTACCAAG
    TACCAGCTGC TCCTGAAGGA ACTTTTAACT TGCTGTGAAG AAGGAAAAGG GGAGCTCAAG
    GAAGGCCTGG AGGTGATGCT TAGTGTCCCA AAGAGAGCCA ACGATGCCAT GCACGTCAGC
    ATGCTGGAAG GGTTCGACGA GAACCTGGAC GTGCAGGGGG AGCTGATTTT ACAAGATGCC
    TTTCAAGTGT GGGACCCCAA GTCGCTGATC CGGAAAGGGC GGGAGCGACA CCTATTCCTC
    TTTGAGATCT CCTTGGTTTT CAGCAAGGAG ATCAAAGACT CTTCAGGACA CACAAAATAT
    GTTTACAAGA ACAAGCTACT GACCTCAGAG CTGGGTGTGA CCGAACACGT AGAGGGCGAT
    CCCTGCAAAT TCGCCTTGTG GTCTGGGCGC ACCCCATCCT CAGACAATAA AACAGTGCTG
    AAAGCCTCCA ACATAGAAAC CAAGCAGGAG TGGATCAAGA ACATTCGAGA GGTGATTCAA
    GAAAGGATCA TTCACCTGAA AGGAGCTTTG AAGGAGCCAA TTCAGCTCCC CAAAACACCA
    GCCAAACAGA GGAACAACAG TAAGAGGGAT GGAGTGGAGG ATGTTGACAG CCAGGGGGAT
    GGGAGCAGCC AGCCAGACAC CATCTCCATT GCCTCTAGGA CCTCCCAGAA CACAGCGGAC
    AGTGACAAGG ATGGCAACCT TGCTCCTCGG TGGTACCTGG GACCTGGAGA TCCTTTCTCC
    ACTTACGTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218894
    Clone ID Related Accession (Same CDS sequence) XM_024991369.1
    Accession Version XM_024991369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CACACCAGAG CGCCCTGCAG
    GTTCAGCAGA AAGCTGAGGC GCTGCTCCAG GCTGGTCACT ATGATGCAGA TGCCATCCGG
    GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAGCAACTCA TGCTCAAGAT GGAAGACCGA
    CTGAAACTGG TCAATGCCTC AGTGGCCTTT TATAAAACTT CTGAGCAGGT CTGTAGTGTC
    CTGGAGAGCT TAGAGCAAGA ATACCGAAGA GATGAGGACT GGTGTGGTGG ACGAGATAAA
    TTGGGGCCAG CAGCAGAGAT CGACCACGTC ATTCCACTCA TCAGTAAACA TTTGGAACAA
    AAGGAAGCCT TTCTTAAGGC CTGCACCCTG GCACGGCGGA ATGCTGAGGT CTTTCTCAAG
    TACATCCATA GGAACAACGT CAGCATGCCC AGTGCTGCCA GCCACACCCG AGGACCCGAA
    CAGCAAGTGA AAGCCATCCT GAGTGAGCTC TTACAGAGGG AGAACCGTGT CCTGCACTTC
    TGGACATTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ACGTGGTATT TGAGCGAAGC
    GCCAAGCAGG CACTAGACTG GATCCAAGAA ACAGGTGAAT ATTACCTCTC AACACATACC
    TCCACCGGGG AGACCACAGA GGAGACTCAG GAACTGCTGA AAGAATATGG GGAGTTCAGG
    GTGCCTGCCA AGCAAACAAA GGAGAAGGTG AAGCTTCTGA TTCAGCTGGC TGATAGCTTT
    GTGGAAAAAG GTCACATTCA TGCCACAGAG ATCAGGAAAT GGGTGACCAC AGTGGACAAG
    CATTACAGAG ACTTCTCACT GAGGATGGGA AAATACCGGT ACTCCCTGGA GAAGGCCCTA
    GGAGTCAACA CGGAGGATAA CAAGGACCTG GAGCTGGACA TCATCCCAGC AAGCCTTTCA
    GATCGTGAGG TCAAGCTCCG GGATGCCAAC CACGAGGTCA ATGAAGAGAA GCGGAAATCA
    GCCAGGAAAA AAGAATTCAT TATGGCAGAA TTGCTCCAGA CAGAGAAGGC TTATGTGAGG
    GACTTACATG AGTGCTTAGA GACCTACCTG TGGGAAATGA CCAGTGGTGT GGAGGAGATC
    CCCCCTGGGA TCCTTAATAA GGAGCATATC ATCTTTGGCA ACATCCAAGA GATCTACGAT
    TTTCATAACA ACATCTTCCT CAAAGAACTG GAGAAGTATG AGCAGCTGCC TGAGGATGTG
    GGACACTGCT TTGTTACTTG GGCAGACAAA TTTCAGATGT ACGTCACCTA CTGTAAAAAC
    AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCAGGCA CCTTCTTTGA TGAGATACAG
    CAGCGGCATG GTCTGGCCAA CTCCATTTCT TCCTACCTAA TTAAGCCTGT CCAGAGGATT
    ACCAAGTACC AGCTGCTCCT GAAGGAACTT TTAACTTGCT GTGAAGAAGG AAAAGGGGAG
    CTCAAGGAAG GCCTGGAGGT GATGCTTAGT GTCCCAAAGA GAGCCAACGA TGCCATGCAC
    GTCAGCATGC TGGAAGGGTT CGACGAGAAC CTGGACGTGC AGGGGGAGCT GATTTTACAA
    GATGCCTTTC AAGTGTGGGA CCCCAAGTCG CTGATCCGGA AAGGGCGGGA GCGACACCTA
    TTCCTCTTTG AGATCTCCTT GGTTTTCAGC AAGGAGATCA AAGACTCTTC AGGACACACA
    AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA ACACGTAGAG
    GGCGATCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA
    GTGCTGAAAG CCTCCAACAT AGAAACCAAG CAGGAGTGGA TCAAGAACAT TCGAGAGGTG
    ATTCAAGAAA GGATCATTCA CCTGAAAGGA GCTTTGAAGG AGCCAATTCA GCTCCCCAAA
    ACACCAGCCA AACAGAGGAA CAACAGTAAG AGGGATGGAG TGGAGGATGT TGACAGCCAG
    GGGGATGGGA GCAGCCAGCC AGACACCATC TCCATTGCCT CTAGGACCTC CCAGAACACA
    GCGGACAGTG ACAAGCTCTC TGGCGGCTGT GAGTTGACCG TGGTGCTCCA GGACTTCAAC
    GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGACAGA CGGTGGAGCT GCTAGAGCGG
    CCGAGCGAGC GGCCGGGCTG GTGTCTGGTC CGAACCACAG AGCGGAGCCC CCCCCAGGAG
    GGCCTGGTGC CCAGCAGCGC CCTGTGCATC TCCCACTCCC GAAGCAGCGT GGAGATGGAC
    TGCTTCTTCC CCCTGGTGAA AGATGCGTAC TCTCATTCCT CAAGTGAAAA CGGAGGCAAG
    TCCGAGTCGG TGGCCAACCT GCAGGCCCAA CCATCCCTGA ACTCCATCCA CAGCTCCCCT
    GGGCCCAAGC GTTCCACCAA CACCCTGAAG AAGTGGCTGA CGAGTCCCGT GCGCCGGCTT
    AACAGTGGGA AAGCAGATGG AAACATCAAG AAGCAGAAGA AAGTACGAGA TGGTCGGAAG
    AGCTTTGACC TGGGATCTCC CAAGCCTGGG GATGAGACGA CCCCTCAGGG AGACAGCGCT
    GATGAGAAGA GCAAGAAAGG TTGGGGTGAA GATGAGGCAG ATGAAGAGTC ACACACACCC
    CTCCCCCCGC CTATGAAGAT CTTTGACAAC GACCCCACGC AGGACGAAAT GTCCTCCTCT
    TTGCTAGCAG CCCGGCAGGC TTCCACCGAA GTACCTACTG CTGCAGACCT TGTCAGTGCG
    ATAGAACAGT TGGTCAAAAG CAAGCTGAGT CTGGAAGGAG GCTCTTACCG AGGGAGCTTG
    AAAGACCCCA CAGGCTGCCT GAATGAAGGC ATGACCCCAC CCACACCTCC CAGAAATCTG
    GAAGAAGAAC AGAAAGCCAA GGCCCTGAGA GGCAGGATGT TTGTCCTGAA TGAACTGGTA
    CAAACAGAGA AGGACTATGT CAAGGACCTG GGAATTGTGG TGGAGGGTTT CATGAAGAGA
    ATAGAAGAAA AGGGTGTCCC CGAGGACATG CGAGGAAAGG ATAAAATCGT GTTTGGAAAT
    ATTCACCAGA TTTATGATTG GCATAAGGAT GTTTTCCTGG GTGAACTGGA AAAATGTATC
    CAGGAGCAAG ACAGATTGGC ACAGCTCTTT ATTAAATATG AGCGGAAGCT GCACATCTAT
    GTGTGGTACT GCCAGAACAA GCCGCGCTCC GAGTACATCG TCGCCGAGTA CGACGCCTAC
    TTTGAAGAGG TGAAACAAGA GATAAATCAA AGGCTGACGC TTAGCGACTT CCTTATCAAG
    CCCATTCAGA GAATAACAAA ATATCAATTG CTCCTCAAGG ACTTCCTGAG ATACAGCGAG
    AAGGCTGGTT TGGAGTGTTC AGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTTCCA
    AAACGCTGCA ATGACATGAT GAACCTGGGG CGCCTGCAGG GCTTTGAGGG GACCCTGACT
    GCTCAGGGCA AGCTGCTGCA GCAGGACACA TTCTACGTGA TTGAGCTGGA CGCTGGCATG
    CAGTCGCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AAATCGTCAT CTTCAGTGAA
    CTGCTCAGGA AGGGATCCCT CACCCCAGGC TACATGTTCA AAAGGAGCAT CAAGATGAAC
    TACTTGGTCC TGGAGGAGAA CGTGGACAAT GACCCCTGCA AGTTTGCGCT TCTGAACAGG
    GAGACTTCAG AGAGGGTTAT TCTGCAAGCC GCCAATGCTG ACATCCAGCA GGCCTGGGTG
    CAGGACATCA ACCAAGTCTT AGAAACACAG CGAGACTTCT TAAATGCCCT ACAGTCGCCC
    ATTGAGTATC AGCGGAAAGA AAGGAGCACA GCCGTGATGA GGTCCCAGCC TGCTAGGGTT
    CCCCAAGCCA GCCCCAGGCC CTACTCCTCC ATCCCCGTGG GCTCCGAGAA GCCCCCAAAG
    GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCCAA TGGCAGTCCA
    GGGTTTGACT ACCACCAGGC TGGGGACAAG TTTGAGGCCA GCAAGAATGA CCTGGGAGGC
    TGCAACGGGG CCTCATCCAT GGCCGTGATC AAAGATTATT ATGCACTGAA GGAGAACGAA
    ATCTGTGTGA GCCAAGGTGA GGTGGTCCAG GTCCTCGCCG TCAACCAGCA GAACATGTGC
    CTGGTGTACC AGCCCGCTAG CGACCACTCC CCCGCCGCTG AGGGCTGGGT CCCTGGCAGC
    ATCCTGACGC CCCTCACCAA AGCCATGGCA GCCACAGAAA ATAGTGACGG GAGCATCAAA
    ACCCTACTGA AACCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847137.1
    CDS356..7573
    Translation

    Target ORF information:

    RefSeq Version XM_024991369.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CACACCAGAG CGCCCTGCAG
    GTTCAGCAGA AAGCTGAGGC GCTGCTCCAG GCTGGTCACT ATGATGCAGA TGCCATCCGG
    GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAGCAACTCA TGCTCAAGAT GGAAGACCGA
    CTGAAACTGG TCAATGCCTC AGTGGCCTTT TATAAAACTT CTGAGCAGGT CTGTAGTGTC
    CTGGAGAGCT TAGAGCAAGA ATACCGAAGA GATGAGGACT GGTGTGGTGG ACGAGATAAA
    TTGGGGCCAG CAGCAGAGAT CGACCACGTC ATTCCACTCA TCAGTAAACA TTTGGAACAA
    AAGGAAGCCT TTCTTAAGGC CTGCACCCTG GCACGGCGGA ATGCTGAGGT CTTTCTCAAG
    TACATCCATA GGAACAACGT CAGCATGCCC AGTGCTGCCA GCCACACCCG AGGACCCGAA
    CAGCAAGTGA AAGCCATCCT GAGTGAGCTC TTACAGAGGG AGAACCGTGT CCTGCACTTC
    TGGACATTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ACGTGGTATT TGAGCGAAGC
    GCCAAGCAGG CACTAGACTG GATCCAAGAA ACAGGTGAAT ATTACCTCTC AACACATACC
    TCCACCGGGG AGACCACAGA GGAGACTCAG GAACTGCTGA AAGAATATGG GGAGTTCAGG
    GTGCCTGCCA AGCAAACAAA GGAGAAGGTG AAGCTTCTGA TTCAGCTGGC TGATAGCTTT
    GTGGAAAAAG GTCACATTCA TGCCACAGAG ATCAGGAAAT GGGTGACCAC AGTGGACAAG
    CATTACAGAG ACTTCTCACT GAGGATGGGA AAATACCGGT ACTCCCTGGA GAAGGCCCTA
    GGAGTCAACA CGGAGGATAA CAAGGACCTG GAGCTGGACA TCATCCCAGC AAGCCTTTCA
    GATCGTGAGG TCAAGCTCCG GGATGCCAAC CACGAGGTCA ATGAAGAGAA GCGGAAATCA
    GCCAGGAAAA AAGAATTCAT TATGGCAGAA TTGCTCCAGA CAGAGAAGGC TTATGTGAGG
    GACTTACATG AGTGCTTAGA GACCTACCTG TGGGAAATGA CCAGTGGTGT GGAGGAGATC
    CCCCCTGGGA TCCTTAATAA GGAGCATATC ATCTTTGGCA ACATCCAAGA GATCTACGAT
    TTTCATAACA ACATCTTCCT CAAAGAACTG GAGAAGTATG AGCAGCTGCC TGAGGATGTG
    GGACACTGCT TTGTTACTTG GGCAGACAAA TTTCAGATGT ACGTCACCTA CTGTAAAAAC
    AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCAGGCA CCTTCTTTGA TGAGATACAG
    CAGCGGCATG GTCTGGCCAA CTCCATTTCT TCCTACCTAA TTAAGCCTGT CCAGAGGATT
    ACCAAGTACC AGCTGCTCCT GAAGGAACTT TTAACTTGCT GTGAAGAAGG AAAAGGGGAG
    CTCAAGGAAG GCCTGGAGGT GATGCTTAGT GTCCCAAAGA GAGCCAACGA TGCCATGCAC
    GTCAGCATGC TGGAAGGGTT CGACGAGAAC CTGGACGTGC AGGGGGAGCT GATTTTACAA
    GATGCCTTTC AAGTGTGGGA CCCCAAGTCG CTGATCCGGA AAGGGCGGGA GCGACACCTA
    TTCCTCTTTG AGATCTCCTT GGTTTTCAGC AAGGAGATCA AAGACTCTTC AGGACACACA
    AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA ACACGTAGAG
    GGCGATCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA
    GTGCTGAAAG CCTCCAACAT AGAAACCAAG CAGGAGTGGA TCAAGAACAT TCGAGAGGTG
    ATTCAAGAAA GGATCATTCA CCTGAAAGGA GCTTTGAAGG AGCCAATTCA GCTCCCCAAA
    ACACCAGCCA AACAGAGGAA CAACAGTAAG AGGGATGGAG TGGAGGATGT TGACAGCCAG
    GGGGATGGGA GCAGCCAGCC AGACACCATC TCCATTGCCT CTAGGACCTC CCAGAACACA
    GCGGACAGTG ACAAGCTCTC TGGCGGCTGT GAGTTGACCG TGGTGCTCCA GGACTTCAAC
    GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGACAGA CGGTGGAGCT GCTAGAGCGG
    CCGAGCGAGC GGCCGGGCTG GTGTCTGGTC CGAACCACAG AGCGGAGCCC CCCCCAGGAG
    GGCCTGGTGC CCAGCAGCGC CCTGTGCATC TCCCACTCCC GAAGCAGCGT GGAGATGGAC
    TGCTTCTTCC CCCTGGTGAA AGATGCGTAC TCTCATTCCT CAAGTGAAAA CGGAGGCAAG
    TCCGAGTCGG TGGCCAACCT GCAGGCCCAA CCATCCCTGA ACTCCATCCA CAGCTCCCCT
    GGGCCCAAGC GTTCCACCAA CACCCTGAAG AAGTGGCTGA CGAGTCCCGT GCGCCGGCTT
    AACAGTGGGA AAGCAGATGG AAACATCAAG AAGCAGAAGA AAGTACGAGA TGGTCGGAAG
    AGCTTTGACC TGGGATCTCC CAAGCCTGGG GATGAGACGA CCCCTCAGGG AGACAGCGCT
    GATGAGAAGA GCAAGAAAGG TTGGGGTGAA GATGAGGCAG ATGAAGAGTC ACACACACCC
    CTCCCCCCGC CTATGAAGAT CTTTGACAAC GACCCCACGC AGGACGAAAT GTCCTCCTCT
    TTGCTAGCAG CCCGGCAGGC TTCCACCGAA GTACCTACTG CTGCAGACCT TGTCAGTGCG
    ATAGAACAGT TGGTCAAAAG CAAGCTGAGT CTGGAAGGAG GCTCTTACCG AGGGAGCTTG
    AAAGACCCCA CAGGCTGCCT GAATGAAGGC ATGACCCCAC CCACACCTCC CAGAAATCTG
    GAAGAAGAAC AGAAAGCCAA GGCCCTGAGA GGCAGGATGT TTGTCCTGAA TGAACTGGTA
    CAAACAGAGA AGGACTATGT CAAGGACCTG GGAATTGTGG TGGAGGGTTT CATGAAGAGA
    ATAGAAGAAA AGGGTGTCCC CGAGGACATG CGAGGAAAGG ATAAAATCGT GTTTGGAAAT
    ATTCACCAGA TTTATGATTG GCATAAGGAT GTTTTCCTGG GTGAACTGGA AAAATGTATC
    CAGGAGCAAG ACAGATTGGC ACAGCTCTTT ATTAAATATG AGCGGAAGCT GCACATCTAT
    GTGTGGTACT GCCAGAACAA GCCGCGCTCC GAGTACATCG TCGCCGAGTA CGACGCCTAC
    TTTGAAGAGG TGAAACAAGA GATAAATCAA AGGCTGACGC TTAGCGACTT CCTTATCAAG
    CCCATTCAGA GAATAACAAA ATATCAATTG CTCCTCAAGG ACTTCCTGAG ATACAGCGAG
    AAGGCTGGTT TGGAGTGTTC AGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTTCCA
    AAACGCTGCA ATGACATGAT GAACCTGGGG CGCCTGCAGG GCTTTGAGGG GACCCTGACT
    GCTCAGGGCA AGCTGCTGCA GCAGGACACA TTCTACGTGA TTGAGCTGGA CGCTGGCATG
    CAGTCGCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AAATCGTCAT CTTCAGTGAA
    CTGCTCAGGA AGGGATCCCT CACCCCAGGC TACATGTTCA AAAGGAGCAT CAAGATGAAC
    TACTTGGTCC TGGAGGAGAA CGTGGACAAT GACCCCTGCA AGTTTGCGCT TCTGAACAGG
    GAGACTTCAG AGAGGGTTAT TCTGCAAGCC GCCAATGCTG ACATCCAGCA GGCCTGGGTG
    CAGGACATCA ACCAAGTCTT AGAAACACAG CGAGACTTCT TAAATGCCCT ACAGTCGCCC
    ATTGAGTATC AGCGGAAAGA AAGGAGCACA GCCGTGATGA GGTCCCAGCC TGCTAGGGTT
    CCCCAAGCCA GCCCCAGGCC CTACTCCTCC ATCCCCGTGG GCTCCGAGAA GCCCCCAAAG
    GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCCAA TGGCAGTCCA
    GGGTTTGACT ACCACCAGGC TGGGGACAAG TTTGAGGCCA GCAAGAATGA CCTGGGAGGC
    TGCAACGGGG CCTCATCCAT GGCCGTGATC AAAGATTATT ATGCACTGAA GGAGAACGAA
    ATCTGTGTGA GCCAAGGTGA GGTGGTCCAG GTCCTCGCCG TCAACCAGCA GAACATGTGC
    CTGGTGTACC AGCCCGCTAG CGACCACTCC CCCGCCGCTG AGGGCTGGGT CCCTGGCAGC
    ATCCTGACGC CCCTCACCAA AGCCATGGCA GCCACAGAAA ATAGTGACGG GAGCATCAAA
    ACCCTACTGA AACCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218895
    Clone ID Related Accession (Same CDS sequence) XM_024991371.1
    Accession Version XM_024991371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CACACCAGAG CGCCCTGCAG
    GTTCAGCAGA AAGCTGAGGC GCTGCTCCAG GCTGGTCACT ATGATGCAGA TGCCATCCGG
    GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAGCAACTCA TGCTCAAGAT GGAAGACCGA
    CTGAAACTGG TCAATGCCTC AGTGGCCTTT TATAAAACTT CTGAGCAGGT CTGTAGTGTC
    CTGGAGAGCT TAGAGCAAGA ATACCGAAGA GATGAGGACT GGTGTGGTGG ACGAGATAAA
    TTGGGGCCAG CAGCAGAGAT CGACCACGTC ATTCCACTCA TCAGTAAACA TTTGGAACAA
    AAGGAAGCCT TTCTTAAGGC CTGCACCCTG GCACGGCGGA ATGCTGAGGT CTTTCTCAAG
    TACATCCATA GGAACAACGT CAGCATGCCC AGTGCTGCCA GCCACACCCG AGGACCCGAA
    CAGCAAGTGA AAGCCATCCT GAGTGAGCTC TTACAGAGGG AGAACCGTGT CCTGCACTTC
    TGGACATTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ACGTGGTATT TGAGCGAAGC
    GCCAAGCAGG CACTAGACTG GATCCAAGAA ACAGGTGAAT ATTACCTCTC AACACATACC
    TCCACCGGGG AGACCACAGA GGAGACTCAG GAACTGCTGA AAGAATATGG GGAGTTCAGG
    GTGCCTGCCA AGCAAACAAA GGAGAAGGTG AAGCTTCTGA TTCAGCTGGC TGATAGCTTT
    GTGGAAAAAG GTCACATTCA TGCCACAGAG ATCAGGAAAT GGGTGACCAC AGTGGACAAG
    CATTACAGAG ACTTCTCACT GAGGATGGGA AAATACCGGT ACTCCCTGGA GAAGGCCCTA
    GGAGTCAACA CGGAGGATAA CAAGGACCTG GAGCTGGACA TCATCCCAGC AAGCCTTTCA
    GATCGTGAGG TCAAGCTCCG GGATGCCAAC CACGAGGTCA ATGAAGAGAA GCGGAAATCA
    GCCAGGAAAA AAGAATTCAT TATGGCAGAA TTGCTCCAGA CAGAGAAGGC TTATGTGAGG
    GACTTACATG AGTGCTTAGA GACCTACCTG TGGGAAATGA CCAGTGGTGT GGAGGAGATC
    CCCCCTGGGA TCCTTAATAA GGAGCATATC ATCTTTGGCA ACATCCAAGA GATCTACGAT
    TTTCATAACA ACATCTTCCT CAAAGAACTG GAGAAGTATG AGCAGCTGCC TGAGGATGTG
    GGACACTGCT TTGTTACTTG GGCAGACAAA TTTCAGATGT ACGTCACCTA CTGTAAAAAC
    AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCAGGCA CCTTCTTTGA TGAGATACAG
    CAGCGGCATG GTCTGGCCAA CTCCATTTCT TCCTACCTAA TTAAGCCTGT CCAGAGGATT
    ACCAAGTACC AGCTGCTCCT GAAGGAACTT TTAACTTGCT GTGAAGAAGG AAAAGGGGAG
    CTCAAGGAAG GCCTGGAGGT GATGCTTAGT GTCCCAAAGA GAGCCAACGA TGCCATGCAC
    GTCAGCATGC TGGAAGGGTT CGACGAGAAC CTGGACGTGC AGGGGGAGCT GATTTTACAA
    GATGCCTTTC AAGTGTGGGA CCCCAAGTCG CTGATCCGGA AAGGGCGGGA GCGACACCTA
    TTCCTCTTTG AGATCTCCTT GGTTTTCAGC AAGGAGATCA AAGACTCTTC AGGACACACA
    AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA ACACGTAGAG
    GGCGATCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA
    GTGCTGAAAG CCTCCAACAT AGAAACCAAG CAGGAGTGGA TCAAGAACAT TCGAGAGGTG
    ATTCAAGAAA GGATCATTCA CCTGAAAGGA GCTTTGAAGG AGCCAATTCA GCTCCCCAAA
    ACACCAGCCA AACAGAGGAA CAACAGTAAG AGGGATGGAG TGGAGGATGT TGACAGCCAG
    GGGGATGGGA GCAGCCAGCC AGACACCATC TCCATTGCCT CTAGGACCTC CCAGAACACA
    GCGGACAGTG ACAAGCTCTC TGGCGGCTGT GAGTTGACCG TGGTGCTCCA GGACTTCAAC
    GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGACAGA CGGTGGAGCT GCTAGAGCGG
    CCGAGCGAGC GGCCGGGCTG GTGTCTGGTC CGAACCACAG AGCGGAGCCC CCCCCAGGAG
    GGCCTGGTGC CCAGCAGCGC CCTGTGCATC TCCCACTCCC GAAGCAGCGT GGAGATGGAC
    TGCTTCTTCC CCCTGGTGAA AGATGCGTAC TCTCATTCCT CAAGTGAAAA CGGAGGCAAG
    TCCGAGTCGG TGGCCAACCT GCAGGCCCAA CCATCCCTGA ACTCCATCCA CAGCTCCCCT
    GGGCCCAAGC GTTCCACCAA CACCCTGAAG AAGTGGCTGA CGAGTCCCGT GCGCCGGCTT
    AACAGTGGGA AAGCAGATGG AAACATCAAG AAGCAGAAGA AAGTACGAGA TGGTCGGAAG
    AGCTTTGACC TGGGATCTCC CAAGCCTGGG GATGAGACGA CCCCTCAGGG AGACAGCGCT
    GATGAGAAGA GCAAGAAAGG TTGGGGTGAA GATGAGGCAG ATGAAGAGTC ACACACACCC
    CTCCCCCCGC CTATGAAGAT CTTTGACAAC GACCCCACGC AGGACGAAAT GTCCTCCTCT
    TTGCTAGCAG CCCGGCAGGC TTCCACCGAA GTACCTACTG CTGCAGACCT TGTCAGTGCG
    ATAGAACAGT TGGTCAAAAG CAAGCTGAGT CTGGAAGGAG GCTCTTACCG AGGGAGCTTG
    AAAGACCCCA CAGGCTGCCT GAATGAAGGC ATGACCCCAC CCACACCTCC CAGAAATCTG
    GAAGAAGAAC AGAAAGCCAA GGCCCTGAGA GGCAGGATGT TTGTCCTGAA TGAACTGGTA
    CAAACAGAGA AGGACTATGT CAAGGACCTG GGAATTGTGG TGGAGGGTTT CATGAAGAGA
    ATAGAAGAAA AGGGTGTCCC CGAGGACATG CGAGGAAAGG ATAAAATCGT GTTTGGAAAT
    ATTCACCAGA TTTATGATTG GCATAAGGAT GTTTTCCTGG GTGAACTGGA AAAATGTATC
    CAGGAGCAAG ACAGATTGGC ACAGCTCTTT ATTAAATATG AGCGGAAGCT GCACATCTAT
    GTGTGGTACT GCCAGAACAA GCCGCGCTCC GAGTACATCG TCGCCGAGTA CGACGCCTAC
    TTTGAAGAGG TGAAACAAGA GATAAATCAA AGGCTGACGC TTAGCGACTT CCTTATCAAG
    CCCATTCAGA GAATAACAAA ATATCAATTG CTCCTCAAGG ACTTCCTGAG ATACAGCGAG
    AAGGCTGGTT TGGAGTGTTC AGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTTCCA
    AAACGCTGCA ATGACATGAT GAACCTGGGG CGCCTGCAGG GCTTTGAGGG GACCCTGACT
    GCTCAGGGCA AGCTGCTGCA GCAGGACACA TTCTACGTGA TTGAGCTGGA CGCTGGCATG
    CAGTCGCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AAATCGTCAT CTTCAGTGAA
    CTGCTCAGGA AGGGATCCCT CACCCCAGGC TACATGTTCA AAAGGAGCAT CAAGATGAAC
    TACTTGGTCC TGGAGGAGAA CGTGGACAAT GACCCCTGCA AGTTTGCGCT TCTGAACAGG
    GAGACTTCAG AGAGGGTTAT TCTGCAAGCC GCCAATGCTG ACATCCAGCA GGCCTGGGTG
    CAGGACATCA ACCAAGTCTT AGAAACACAG CGAGACTTCT TAAATGCCCT ACAGTCGCCC
    ATTGAGTATC AGCGGAAAGA AAGGAGCACA GCCGTGATGA GGTCCCAGCC TGCTAGGGTT
    CCCCAAGCCA GCCCCAGGCC CTACTCCTCC ATCCCCGTGG GCTCCGAGAA GCCCCCAAAG
    GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCCAA TGGCAGTCCA
    GGGTTTGACT ACCACCAGGC TGGGGACAAG TTTGAGGCCA GCAAGAATGA CCTGGGAGGC
    TGCAACGGGG CCTCATCCAT GGCCGTGATC AAAGATTATT ATGCACTGAA GGAGAACGAA
    ATCTGTGTGA GCCAAGGTGA GGTGGTCCAG GTCCTCGCCG TCAACCAGCA GAACATGTGC
    CTGGTGTACC AGCCCGCTAG CGACCACTCC CCCGCCGCTG AGGGCTGGGT CCCTGGCAGC
    ATCCTGACGC CCCTCACCAA AGCCATGGCA GCCACAGAAA ATAGTGACGG GAGCATCAAA
    AAAGAACCCT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847139.1
    CDS356..7570
    Translation

    Target ORF information:

    RefSeq Version XM_024991371.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CACACCAGAG CGCCCTGCAG
    GTTCAGCAGA AAGCTGAGGC GCTGCTCCAG GCTGGTCACT ATGATGCAGA TGCCATCCGG
    GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAGCAACTCA TGCTCAAGAT GGAAGACCGA
    CTGAAACTGG TCAATGCCTC AGTGGCCTTT TATAAAACTT CTGAGCAGGT CTGTAGTGTC
    CTGGAGAGCT TAGAGCAAGA ATACCGAAGA GATGAGGACT GGTGTGGTGG ACGAGATAAA
    TTGGGGCCAG CAGCAGAGAT CGACCACGTC ATTCCACTCA TCAGTAAACA TTTGGAACAA
    AAGGAAGCCT TTCTTAAGGC CTGCACCCTG GCACGGCGGA ATGCTGAGGT CTTTCTCAAG
    TACATCCATA GGAACAACGT CAGCATGCCC AGTGCTGCCA GCCACACCCG AGGACCCGAA
    CAGCAAGTGA AAGCCATCCT GAGTGAGCTC TTACAGAGGG AGAACCGTGT CCTGCACTTC
    TGGACATTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ACGTGGTATT TGAGCGAAGC
    GCCAAGCAGG CACTAGACTG GATCCAAGAA ACAGGTGAAT ATTACCTCTC AACACATACC
    TCCACCGGGG AGACCACAGA GGAGACTCAG GAACTGCTGA AAGAATATGG GGAGTTCAGG
    GTGCCTGCCA AGCAAACAAA GGAGAAGGTG AAGCTTCTGA TTCAGCTGGC TGATAGCTTT
    GTGGAAAAAG GTCACATTCA TGCCACAGAG ATCAGGAAAT GGGTGACCAC AGTGGACAAG
    CATTACAGAG ACTTCTCACT GAGGATGGGA AAATACCGGT ACTCCCTGGA GAAGGCCCTA
    GGAGTCAACA CGGAGGATAA CAAGGACCTG GAGCTGGACA TCATCCCAGC AAGCCTTTCA
    GATCGTGAGG TCAAGCTCCG GGATGCCAAC CACGAGGTCA ATGAAGAGAA GCGGAAATCA
    GCCAGGAAAA AAGAATTCAT TATGGCAGAA TTGCTCCAGA CAGAGAAGGC TTATGTGAGG
    GACTTACATG AGTGCTTAGA GACCTACCTG TGGGAAATGA CCAGTGGTGT GGAGGAGATC
    CCCCCTGGGA TCCTTAATAA GGAGCATATC ATCTTTGGCA ACATCCAAGA GATCTACGAT
    TTTCATAACA ACATCTTCCT CAAAGAACTG GAGAAGTATG AGCAGCTGCC TGAGGATGTG
    GGACACTGCT TTGTTACTTG GGCAGACAAA TTTCAGATGT ACGTCACCTA CTGTAAAAAC
    AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCAGGCA CCTTCTTTGA TGAGATACAG
    CAGCGGCATG GTCTGGCCAA CTCCATTTCT TCCTACCTAA TTAAGCCTGT CCAGAGGATT
    ACCAAGTACC AGCTGCTCCT GAAGGAACTT TTAACTTGCT GTGAAGAAGG AAAAGGGGAG
    CTCAAGGAAG GCCTGGAGGT GATGCTTAGT GTCCCAAAGA GAGCCAACGA TGCCATGCAC
    GTCAGCATGC TGGAAGGGTT CGACGAGAAC CTGGACGTGC AGGGGGAGCT GATTTTACAA
    GATGCCTTTC AAGTGTGGGA CCCCAAGTCG CTGATCCGGA AAGGGCGGGA GCGACACCTA
    TTCCTCTTTG AGATCTCCTT GGTTTTCAGC AAGGAGATCA AAGACTCTTC AGGACACACA
    AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA ACACGTAGAG
    GGCGATCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA
    GTGCTGAAAG CCTCCAACAT AGAAACCAAG CAGGAGTGGA TCAAGAACAT TCGAGAGGTG
    ATTCAAGAAA GGATCATTCA CCTGAAAGGA GCTTTGAAGG AGCCAATTCA GCTCCCCAAA
    ACACCAGCCA AACAGAGGAA CAACAGTAAG AGGGATGGAG TGGAGGATGT TGACAGCCAG
    GGGGATGGGA GCAGCCAGCC AGACACCATC TCCATTGCCT CTAGGACCTC CCAGAACACA
    GCGGACAGTG ACAAGCTCTC TGGCGGCTGT GAGTTGACCG TGGTGCTCCA GGACTTCAAC
    GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGACAGA CGGTGGAGCT GCTAGAGCGG
    CCGAGCGAGC GGCCGGGCTG GTGTCTGGTC CGAACCACAG AGCGGAGCCC CCCCCAGGAG
    GGCCTGGTGC CCAGCAGCGC CCTGTGCATC TCCCACTCCC GAAGCAGCGT GGAGATGGAC
    TGCTTCTTCC CCCTGGTGAA AGATGCGTAC TCTCATTCCT CAAGTGAAAA CGGAGGCAAG
    TCCGAGTCGG TGGCCAACCT GCAGGCCCAA CCATCCCTGA ACTCCATCCA CAGCTCCCCT
    GGGCCCAAGC GTTCCACCAA CACCCTGAAG AAGTGGCTGA CGAGTCCCGT GCGCCGGCTT
    AACAGTGGGA AAGCAGATGG AAACATCAAG AAGCAGAAGA AAGTACGAGA TGGTCGGAAG
    AGCTTTGACC TGGGATCTCC CAAGCCTGGG GATGAGACGA CCCCTCAGGG AGACAGCGCT
    GATGAGAAGA GCAAGAAAGG TTGGGGTGAA GATGAGGCAG ATGAAGAGTC ACACACACCC
    CTCCCCCCGC CTATGAAGAT CTTTGACAAC GACCCCACGC AGGACGAAAT GTCCTCCTCT
    TTGCTAGCAG CCCGGCAGGC TTCCACCGAA GTACCTACTG CTGCAGACCT TGTCAGTGCG
    ATAGAACAGT TGGTCAAAAG CAAGCTGAGT CTGGAAGGAG GCTCTTACCG AGGGAGCTTG
    AAAGACCCCA CAGGCTGCCT GAATGAAGGC ATGACCCCAC CCACACCTCC CAGAAATCTG
    GAAGAAGAAC AGAAAGCCAA GGCCCTGAGA GGCAGGATGT TTGTCCTGAA TGAACTGGTA
    CAAACAGAGA AGGACTATGT CAAGGACCTG GGAATTGTGG TGGAGGGTTT CATGAAGAGA
    ATAGAAGAAA AGGGTGTCCC CGAGGACATG CGAGGAAAGG ATAAAATCGT GTTTGGAAAT
    ATTCACCAGA TTTATGATTG GCATAAGGAT GTTTTCCTGG GTGAACTGGA AAAATGTATC
    CAGGAGCAAG ACAGATTGGC ACAGCTCTTT ATTAAATATG AGCGGAAGCT GCACATCTAT
    GTGTGGTACT GCCAGAACAA GCCGCGCTCC GAGTACATCG TCGCCGAGTA CGACGCCTAC
    TTTGAAGAGG TGAAACAAGA GATAAATCAA AGGCTGACGC TTAGCGACTT CCTTATCAAG
    CCCATTCAGA GAATAACAAA ATATCAATTG CTCCTCAAGG ACTTCCTGAG ATACAGCGAG
    AAGGCTGGTT TGGAGTGTTC AGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTTCCA
    AAACGCTGCA ATGACATGAT GAACCTGGGG CGCCTGCAGG GCTTTGAGGG GACCCTGACT
    GCTCAGGGCA AGCTGCTGCA GCAGGACACA TTCTACGTGA TTGAGCTGGA CGCTGGCATG
    CAGTCGCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AAATCGTCAT CTTCAGTGAA
    CTGCTCAGGA AGGGATCCCT CACCCCAGGC TACATGTTCA AAAGGAGCAT CAAGATGAAC
    TACTTGGTCC TGGAGGAGAA CGTGGACAAT GACCCCTGCA AGTTTGCGCT TCTGAACAGG
    GAGACTTCAG AGAGGGTTAT TCTGCAAGCC GCCAATGCTG ACATCCAGCA GGCCTGGGTG
    CAGGACATCA ACCAAGTCTT AGAAACACAG CGAGACTTCT TAAATGCCCT ACAGTCGCCC
    ATTGAGTATC AGCGGAAAGA AAGGAGCACA GCCGTGATGA GGTCCCAGCC TGCTAGGGTT
    CCCCAAGCCA GCCCCAGGCC CTACTCCTCC ATCCCCGTGG GCTCCGAGAA GCCCCCAAAG
    GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCCAA TGGCAGTCCA
    GGGTTTGACT ACCACCAGGC TGGGGACAAG TTTGAGGCCA GCAAGAATGA CCTGGGAGGC
    TGCAACGGGG CCTCATCCAT GGCCGTGATC AAAGATTATT ATGCACTGAA GGAGAACGAA
    ATCTGTGTGA GCCAAGGTGA GGTGGTCCAG GTCCTCGCCG TCAACCAGCA GAACATGTGC
    CTGGTGTACC AGCCCGCTAG CGACCACTCC CCCGCCGCTG AGGGCTGGGT CCCTGGCAGC
    ATCCTGACGC CCCTCACCAA AGCCATGGCA GCCACAGAAA ATAGTGACGG GAGCATCAAA
    AAAGAACCCT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218896
    Clone ID Related Accession (Same CDS sequence) XM_024991374.1
    Accession Version XM_024991374.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CACACCAGAG CGCCCTGCAG
    GTTCAGCAGA AAGCTGAGGC GCTGCTCCAG GCTGGTCACT ATGATGCAGA TGCCATCCGG
    GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAGCAACTCA TGCTCAAGAT GGAAGACCGA
    CTGAAACTGG TCAATGCCTC AGTGGCCTTT TATAAAACTT CTGAGCAGGT CTGTAGTGTC
    CTGGAGAGCT TAGAGCAAGA ATACCGAAGA GATGAGGACT GGTGTGGTGG ACGAGATAAA
    TTGGGGCCAG CAGCAGAGAT CGACCACGTC ATTCCACTCA TCAGTAAACA TTTGGAACAA
    AAGGAAGCCT TTCTTAAGGC CTGCACCCTG GCACGGCGGA ATGCTGAGGT CTTTCTCAAG
    TACATCCATA GGAACAACGT CAGCATGCCC AGTGCTGCCA GCCACACCCG AGGACCCGAA
    CAGCAAGTGA AAGCCATCCT GAGTGAGCTC TTACAGAGGG AGAACCGTGT CCTGCACTTC
    TGGACATTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ACGTGGTATT TGAGCGAAGC
    GCCAAGCAGG CACTAGACTG GATCCAAGAA ACAGGTGAAT ATTACCTCTC AACACATACC
    TCCACCGGGG AGACCACAGA GGAGACTCAG GAACTGCTGA AAGAATATGG GGAGTTCAGG
    GTGCCTGCCA AGCAAACAAA GGAGAAGGTG AAGCTTCTGA TTCAGCTGGC TGATAGCTTT
    GTGGAAAAAG GTCACATTCA TGCCACAGAG ATCAGGAAAT GGGTGACCAC AGTGGACAAG
    CATTACAGAG ACTTCTCACT GAGGATGGGA AAATACCGGT ACTCCCTGGA GAAGGCCCTA
    GGAGTCAACA CGGAGGATAA CAAGGACCTG GAGCTGGACA TCATCCCAGC AAGCCTTTCA
    GATCGTGAGG TCAAGCTCCG GGATGCCAAC CACGAGGTCA ATGAAGAGAA GCGGAAATCA
    GCCAGGAAAA AAGAATTCAT TATGGCAGAA TTGCTCCAGA CAGAGAAGGC TTATGTGAGG
    GACTTACATG AGTGCTTAGA GACCTACCTG TGGGAAATGA CCAGTGGTGT GGAGGAGATC
    CCCCCTGGGA TCCTTAATAA GGAGCATATC ATCTTTGGCA ACATCCAAGA GATCTACGAT
    TTTCATAACA ACATCTTCCT CAAAGAACTG GAGAAGTATG AGCAGCTGCC TGAGGATGTG
    GGACACTGCT TTGTTACTTG GGCAGACAAA TTTCAGATGT ACGTCACCTA CTGTAAAAAC
    AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCAGGCA CCTTCTTTGA TGAGATACAG
    CAGCGGCATG GTCTGGCCAA CTCCATTTCT TCCTACCTAA TTAAGCCTGT CCAGAGGATT
    ACCAAGTACC AGCTGCTCCT GAAGGAACTT TTAACTTGCT GTGAAGAAGG AAAAGGGGAG
    CTCAAGGAAG GCCTGGAGGT GATGCTTAGT GTCCCAAAGA GAGCCAACGA TGCCATGCAC
    GTCAGCATGC TGGAAGGGTT CGACGAGAAC CTGGACGTGC AGGGGGAGCT GATTTTACAA
    GATGCCTTTC AAGTGTGGGA CCCCAAGTCG CTGATCCGGA AAGGGCGGGA GCGACACCTA
    TTCCTCTTTG AGATCTCCTT GGTTTTCAGC AAGGAGATCA AAGACTCTTC AGGACACACA
    AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA ACACGTAGAG
    GGCGATCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA
    GTGCTGAAAG CCTCCAACAT AGAAACCAAG CAGGAGTGGA TCAAGAACAT TCGAGAGGTG
    ATTCAAGAAA GGATCATTCA CCTGAAAGGA GCTTTGAAGG AGCCAATTCA GCTCCCCAAA
    ACACCAGCCA AACAGAGGAA CAACAGTAAG AGGGATGGAG TGGAGGATGT TGACAGCCAG
    GGGGATGGGA GCAGCCAGCC AGACACCATC TCCATTGCCT CTAGGACCTC CCAGAACACA
    GCGGACAGTG ACAAGCTCTC TGGCGGCTGT GAGTTGACCG TGGTGCTCCA GGACTTCAAC
    GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGACAGA CGGTGGAGCT GCTAGAGCGG
    CCGAGCGAGC GGCCGGGCTG GTGTCTGGTC CGAACCACAG AGCGGAGCCC CCCCCAGGAG
    GGCCTGGTGC CCAGCAGCGC CCTGTGCATC TCCCACTCCC GAAGCAGCGT GGAGATGGAC
    TGCTTCTTCC CCCTGGTGAA AGATGCGTAC TCTCATTCCT CAAGTGAAAA CGGAGGCAAG
    TCCGAGTCGG TGGCCAACCT GCAGGCCCAA CCATCCCTGA ACTCCATCCA CAGCTCCCCT
    GGGCCCAAGC GTTCCACCAA CACCCTGAAG AAGTGGCTGA CGAGTCCCGT GCGCCGGCTT
    AACAGTGGGA AAGCAGATGG AAACATCAAG AAGCAGAAGA AAGTACGAGA TGGTCGGAAG
    AGCTTTGACC TGGGATCTCC CAAGCCTGGG GATGAGACGA CCCCTCAGGG AGACAGCGCT
    GATGAGAGCA AGAAAGGTTG GGGTGAAGAT GAGGCAGATG AAGAGTCACA CACACCCCTC
    CCCCCGCCTA TGAAGATCTT TGACAACGAC CCCACGCAGG ACGAAATGTC CTCCTCTTTG
    CTAGCAGCCC GGCAGGCTTC CACCGAAGTA CCTACTGCTG CAGACCTTGT CAGTGCGATA
    GAACAGTTGG TCAAAAGCAA GCTGAGTCTG GAAGGAGGCT CTTACCGAGG GAGCTTGAAA
    GACCCCACAG GCTGCCTGAA TGAAGGCATG ACCCCACCCA CACCTCCCAG AAATCTGGAA
    GAAGAACAGA AAGCCAAGGC CCTGAGAGGC AGGATGTTTG TCCTGAATGA ACTGGTACAA
    ACAGAGAAGG ACTATGTCAA GGACCTGGGA ATTGTGGTGG AGGGTTTCAT GAAGAGAATA
    GAAGAAAAGG GTGTCCCCGA GGACATGCGA GGAAAGGATA AAATCGTGTT TGGAAATATT
    CACCAGATTT ATGATTGGCA TAAGGATGTT TTCCTGGGTG AACTGGAAAA ATGTATCCAG
    GAGCAAGACA GATTGGCACA GCTCTTTATT AAATATGAGC GGAAGCTGCA CATCTATGTG
    TGGTACTGCC AGAACAAGCC GCGCTCCGAG TACATCGTCG CCGAGTACGA CGCCTACTTT
    GAAGAGGTGA AACAAGAGAT AAATCAAAGG CTGACGCTTA GCGACTTCCT TATCAAGCCC
    ATTCAGAGAA TAACAAAATA TCAATTGCTC CTCAAGGACT TCCTGAGATA CAGCGAGAAG
    GCTGGTTTGG AGTGTTCAGA TATTGAGAAA GCAGTGGAGT TAATGTGCCT TGTTCCAAAA
    CGCTGCAATG ACATGATGAA CCTGGGGCGC CTGCAGGGCT TTGAGGGGAC CCTGACTGCT
    CAGGGCAAGC TGCTGCAGCA GGACACATTC TACGTGATTG AGCTGGACGC TGGCATGCAG
    TCGCGGACCA AGGAGAGGCG TGTGTTTCTC TTTGAGCAAA TCGTCATCTT CAGTGAACTG
    CTCAGGAAGG GATCCCTCAC CCCAGGCTAC ATGTTCAAAA GGAGCATCAA GATGAACTAC
    TTGGTCCTGG AGGAGAACGT GGACAATGAC CCCTGCAAGT TTGCGCTTCT GAACAGGGAG
    ACTTCAGAGA GGGTTATTCT GCAAGCCGCC AATGCTGACA TCCAGCAGGC CTGGGTGCAG
    GACATCAACC AAGTCTTAGA AACACAGCGA GACTTCTTAA ATGCCCTACA GTCGCCCATT
    GAGTATCAGC GGAAAGAAAG GAGCACAGCC GTGATGAGGT CCCAGCCTGC TAGGGTTCCC
    CAAGCCAGCC CCAGGCCCTA CTCCTCCATC CCCGTGGGCT CCGAGAAGCC CCCAAAGGGC
    TCCAGCTATA ACCCGCCTCT GCCACCCCTG AAGATATCTA CCTCCAATGG CAGTCCAGGG
    TTTGACTACC ACCAGGCTGG GGACAAGTTT GAGGCCAGCA AGAATGACCT GGGAGGCTGC
    AACGGGGCCT CATCCATGGC CGTGATCAAA GATTATTATG CACTGAAGGA GAACGAAATC
    TGTGTGAGCC AAGGTGAGGT GGTCCAGGTC CTCGCCGTCA ACCAGCAGAA CATGTGCCTG
    GTGTACCAGC CCGCTAGCGA CCACTCCCCC GCCGCTGAGG GCTGGGTCCC TGGCAGCATC
    CTGACGCCCC TCACCAAAGC CATGGCAGCC ACAGAAAATA GTGACGGGAG CATCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847142.1
    CDS357..7556
    Translation

    Target ORF information:

    RefSeq Version XM_024991374.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991374.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CACACCAGAG CGCCCTGCAG
    GTTCAGCAGA AAGCTGAGGC GCTGCTCCAG GCTGGTCACT ATGATGCAGA TGCCATCCGG
    GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAGCAACTCA TGCTCAAGAT GGAAGACCGA
    CTGAAACTGG TCAATGCCTC AGTGGCCTTT TATAAAACTT CTGAGCAGGT CTGTAGTGTC
    CTGGAGAGCT TAGAGCAAGA ATACCGAAGA GATGAGGACT GGTGTGGTGG ACGAGATAAA
    TTGGGGCCAG CAGCAGAGAT CGACCACGTC ATTCCACTCA TCAGTAAACA TTTGGAACAA
    AAGGAAGCCT TTCTTAAGGC CTGCACCCTG GCACGGCGGA ATGCTGAGGT CTTTCTCAAG
    TACATCCATA GGAACAACGT CAGCATGCCC AGTGCTGCCA GCCACACCCG AGGACCCGAA
    CAGCAAGTGA AAGCCATCCT GAGTGAGCTC TTACAGAGGG AGAACCGTGT CCTGCACTTC
    TGGACATTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ACGTGGTATT TGAGCGAAGC
    GCCAAGCAGG CACTAGACTG GATCCAAGAA ACAGGTGAAT ATTACCTCTC AACACATACC
    TCCACCGGGG AGACCACAGA GGAGACTCAG GAACTGCTGA AAGAATATGG GGAGTTCAGG
    GTGCCTGCCA AGCAAACAAA GGAGAAGGTG AAGCTTCTGA TTCAGCTGGC TGATAGCTTT
    GTGGAAAAAG GTCACATTCA TGCCACAGAG ATCAGGAAAT GGGTGACCAC AGTGGACAAG
    CATTACAGAG ACTTCTCACT GAGGATGGGA AAATACCGGT ACTCCCTGGA GAAGGCCCTA
    GGAGTCAACA CGGAGGATAA CAAGGACCTG GAGCTGGACA TCATCCCAGC AAGCCTTTCA
    GATCGTGAGG TCAAGCTCCG GGATGCCAAC CACGAGGTCA ATGAAGAGAA GCGGAAATCA
    GCCAGGAAAA AAGAATTCAT TATGGCAGAA TTGCTCCAGA CAGAGAAGGC TTATGTGAGG
    GACTTACATG AGTGCTTAGA GACCTACCTG TGGGAAATGA CCAGTGGTGT GGAGGAGATC
    CCCCCTGGGA TCCTTAATAA GGAGCATATC ATCTTTGGCA ACATCCAAGA GATCTACGAT
    TTTCATAACA ACATCTTCCT CAAAGAACTG GAGAAGTATG AGCAGCTGCC TGAGGATGTG
    GGACACTGCT TTGTTACTTG GGCAGACAAA TTTCAGATGT ACGTCACCTA CTGTAAAAAC
    AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCAGGCA CCTTCTTTGA TGAGATACAG
    CAGCGGCATG GTCTGGCCAA CTCCATTTCT TCCTACCTAA TTAAGCCTGT CCAGAGGATT
    ACCAAGTACC AGCTGCTCCT GAAGGAACTT TTAACTTGCT GTGAAGAAGG AAAAGGGGAG
    CTCAAGGAAG GCCTGGAGGT GATGCTTAGT GTCCCAAAGA GAGCCAACGA TGCCATGCAC
    GTCAGCATGC TGGAAGGGTT CGACGAGAAC CTGGACGTGC AGGGGGAGCT GATTTTACAA
    GATGCCTTTC AAGTGTGGGA CCCCAAGTCG CTGATCCGGA AAGGGCGGGA GCGACACCTA
    TTCCTCTTTG AGATCTCCTT GGTTTTCAGC AAGGAGATCA AAGACTCTTC AGGACACACA
    AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA ACACGTAGAG
    GGCGATCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA
    GTGCTGAAAG CCTCCAACAT AGAAACCAAG CAGGAGTGGA TCAAGAACAT TCGAGAGGTG
    ATTCAAGAAA GGATCATTCA CCTGAAAGGA GCTTTGAAGG AGCCAATTCA GCTCCCCAAA
    ACACCAGCCA AACAGAGGAA CAACAGTAAG AGGGATGGAG TGGAGGATGT TGACAGCCAG
    GGGGATGGGA GCAGCCAGCC AGACACCATC TCCATTGCCT CTAGGACCTC CCAGAACACA
    GCGGACAGTG ACAAGCTCTC TGGCGGCTGT GAGTTGACCG TGGTGCTCCA GGACTTCAAC
    GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGACAGA CGGTGGAGCT GCTAGAGCGG
    CCGAGCGAGC GGCCGGGCTG GTGTCTGGTC CGAACCACAG AGCGGAGCCC CCCCCAGGAG
    GGCCTGGTGC CCAGCAGCGC CCTGTGCATC TCCCACTCCC GAAGCAGCGT GGAGATGGAC
    TGCTTCTTCC CCCTGGTGAA AGATGCGTAC TCTCATTCCT CAAGTGAAAA CGGAGGCAAG
    TCCGAGTCGG TGGCCAACCT GCAGGCCCAA CCATCCCTGA ACTCCATCCA CAGCTCCCCT
    GGGCCCAAGC GTTCCACCAA CACCCTGAAG AAGTGGCTGA CGAGTCCCGT GCGCCGGCTT
    AACAGTGGGA AAGCAGATGG AAACATCAAG AAGCAGAAGA AAGTACGAGA TGGTCGGAAG
    AGCTTTGACC TGGGATCTCC CAAGCCTGGG GATGAGACGA CCCCTCAGGG AGACAGCGCT
    GATGAGAGCA AGAAAGGTTG GGGTGAAGAT GAGGCAGATG AAGAGTCACA CACACCCCTC
    CCCCCGCCTA TGAAGATCTT TGACAACGAC CCCACGCAGG ACGAAATGTC CTCCTCTTTG
    CTAGCAGCCC GGCAGGCTTC CACCGAAGTA CCTACTGCTG CAGACCTTGT CAGTGCGATA
    GAACAGTTGG TCAAAAGCAA GCTGAGTCTG GAAGGAGGCT CTTACCGAGG GAGCTTGAAA
    GACCCCACAG GCTGCCTGAA TGAAGGCATG ACCCCACCCA CACCTCCCAG AAATCTGGAA
    GAAGAACAGA AAGCCAAGGC CCTGAGAGGC AGGATGTTTG TCCTGAATGA ACTGGTACAA
    ACAGAGAAGG ACTATGTCAA GGACCTGGGA ATTGTGGTGG AGGGTTTCAT GAAGAGAATA
    GAAGAAAAGG GTGTCCCCGA GGACATGCGA GGAAAGGATA AAATCGTGTT TGGAAATATT
    CACCAGATTT ATGATTGGCA TAAGGATGTT TTCCTGGGTG AACTGGAAAA ATGTATCCAG
    GAGCAAGACA GATTGGCACA GCTCTTTATT AAATATGAGC GGAAGCTGCA CATCTATGTG
    TGGTACTGCC AGAACAAGCC GCGCTCCGAG TACATCGTCG CCGAGTACGA CGCCTACTTT
    GAAGAGGTGA AACAAGAGAT AAATCAAAGG CTGACGCTTA GCGACTTCCT TATCAAGCCC
    ATTCAGAGAA TAACAAAATA TCAATTGCTC CTCAAGGACT TCCTGAGATA CAGCGAGAAG
    GCTGGTTTGG AGTGTTCAGA TATTGAGAAA GCAGTGGAGT TAATGTGCCT TGTTCCAAAA
    CGCTGCAATG ACATGATGAA CCTGGGGCGC CTGCAGGGCT TTGAGGGGAC CCTGACTGCT
    CAGGGCAAGC TGCTGCAGCA GGACACATTC TACGTGATTG AGCTGGACGC TGGCATGCAG
    TCGCGGACCA AGGAGAGGCG TGTGTTTCTC TTTGAGCAAA TCGTCATCTT CAGTGAACTG
    CTCAGGAAGG GATCCCTCAC CCCAGGCTAC ATGTTCAAAA GGAGCATCAA GATGAACTAC
    TTGGTCCTGG AGGAGAACGT GGACAATGAC CCCTGCAAGT TTGCGCTTCT GAACAGGGAG
    ACTTCAGAGA GGGTTATTCT GCAAGCCGCC AATGCTGACA TCCAGCAGGC CTGGGTGCAG
    GACATCAACC AAGTCTTAGA AACACAGCGA GACTTCTTAA ATGCCCTACA GTCGCCCATT
    GAGTATCAGC GGAAAGAAAG GAGCACAGCC GTGATGAGGT CCCAGCCTGC TAGGGTTCCC
    CAAGCCAGCC CCAGGCCCTA CTCCTCCATC CCCGTGGGCT CCGAGAAGCC CCCAAAGGGC
    TCCAGCTATA ACCCGCCTCT GCCACCCCTG AAGATATCTA CCTCCAATGG CAGTCCAGGG
    TTTGACTACC ACCAGGCTGG GGACAAGTTT GAGGCCAGCA AGAATGACCT GGGAGGCTGC
    AACGGGGCCT CATCCATGGC CGTGATCAAA GATTATTATG CACTGAAGGA GAACGAAATC
    TGTGTGAGCC AAGGTGAGGT GGTCCAGGTC CTCGCCGTCA ACCAGCAGAA CATGTGCCTG
    GTGTACCAGC CCGCTAGCGA CCACTCCCCC GCCGCTGAGG GCTGGGTCCC TGGCAGCATC
    CTGACGCCCC TCACCAAAGC CATGGCAGCC ACAGAAAATA GTGACGGGAG CATCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01748
    Clone ID Related Accession (Same CDS sequence) NM_001191364.1
    Accession Version NM_001191364.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2011-11-04
    Organism Bos taurus(cattle)
    Product kalirin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03074860.1, AAFC03049569.1, AAFC03049571.1, AAFC03053050.1, AAFC03054101.1, AAFC03054102.1, AAFC03054100.1, AAFC03053051.1, AAFC03121339.1, AAFC03121342.1, AAFC03124948.1, AAFC03121341.1, AAFC03097369.1, AAFC03097368.1, AAFC03097366.1, AAFC03121340.1, AAFC03090700.1 and AAFC03090701.1. On Jul 17, 2010 this sequence version replaced XM_001790250.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGACGGACC GCTTCTGGGA ACAGTGGTAT CTTTGGTATC TCCGCTTGCT CCGGCTGCTG 
    GATCGAGGCT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC CATCCTAAAG
    GAAAAAGTGG CCTTCGTGTC TGGGGGCCGT GATAAGCGAG GTGGACCCAT CCTGACCTTC
    CCTGCTCGCA GCAATCATGA CAGAATAAGA CAGGAAGACC TGCGGAAACT GGTGACCTAT
    CTGGCCAGCG TGCCAAGCGA GGACGTGTGC AAACGTGGCT TCACTGTCAT CATCGATATG
    CGGGGGTCCA AGTGGGACCT CATCAAGCCC CTCCTCAAGA CGCTGCAAGA AGCCTTTCCC
    GCTGAGATCC ATGTGGCCCT CATCATCAAA CCCGACAACT TCTGGCAGAA ACAGAAGACC
    AACTTTGGCA GCTCCAAGTT CATCTTTGAG ACAAGCATGG TGTCCGTGGA GGGCCTCACG
    AAGCTGGTGG ACCCCTCCCA GCTGACGGAG GAGTTCGATG GCTCCCTGGA CTACAACCAC
    GAGGAGTGGA TCGAGCTGCG GCTCTCCCTG GAGGAGTTCT TCAACAGCGC CGTACACCTG
    CTCTCACGCC TTGAAGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC CGTGGATGTG
    GAGGGCTCGA GGCGGCTAAT TGATGAACAC ACACAGCTGA AGAAGAAGGT GCTGAAGGCG
    CCTGTGGAGG AGCTGGACCG GGAGGGGCAG CGACTGCTGC AGTGCATCCG CTGCAGTGAC
    GGCTTCTCAG GGCGCAACTG CATCCCGGGC AGTGCCGACT TCCAGAGCTT GGTGCCCAAG
    ATCACCAGCC TGTTGGACAA GCTGCACTCC ACCCGGCAGC ACCTGCACCA GATGTGGCAT
    GTGCGCAAGC TCAAGCTAGA CCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAA
    AAGATGTTTG ACTGGATAAG CCACAACAAG GAGTTATTCC TCCAGAGCCA TACAGAGATT
    GGAGTCAGCT ACCAGCACGC CCTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC
    TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TGGCTTCCCG CCTCTCAGAG
    GCTGGCCACT ATGCTTCACA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG
    AAGAGTTTCG CAGCTGCCCT GGATGAACGC AGCACCATCC TCGCCATGTC TGCTGTGTTC
    CATCAGAAGG CTGAGCAGTT CTTGTCAGGA GTGGATGCCT GGTGCAAGAT GTGCAGTGAA
    GGTGGCCTGC CCTCTGAGAT GCAGGACCTG GAGCTGGCGA TCCACCACCA CCAGTCTTTG
    TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ACGGCAAAGC ACTGCTGGAT
    GTGCTGCAGC GGCCCCTGAG TCCCGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC
    TCCAAGGCGG TGCACCAGGT GCTGGACGTG GTGCACGAAG TGCTGCACCA CCAGCGGCGG
    CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTCCACC AACGCCTGCA GCTCTGCGTC
    TTCCAGCAGG ACGTCCAGCA GGTGTTAGAT TGGATTGAAA ACCACGGCGA GGCCTTTCTC
    AGCAAGCACA CAGGGGTCGG GAAGTCTCTG CACCGAGCTC GGGCCCTGCA GAAGAGGCAC
    GATGACTTTG AAGAGGTGGC TCAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCG
    GCAGAGCAGC TGGCTCAGAC AGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCTAGA
    CACCTGGAGG TGCGCATCCA AGACTTCGTG CGCAGGGTGG AGCAGCGGAA GCTTCTGCTG
    GACATGTCTG TCTCCTTCCA TACTCACACC AAAGAGCTAT GGACATGGAT GGAAGACCTT
    CAGAAGGAGA TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAACTGATC
    AAGCAGTTCC AGCAGCAGCA GACCGCCACT CTGGATGCCA CACTTAATGT CATCAAGGAG
    GGCGAAGACC TTATCCAGCA ACTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCAGCGAG
    GCCAGGGACT CAGCTGTGTC CAACAACAAG ACGCCCCATA GTAGCTCCAT CAGCCACATA
    GAGTCGGTGC TGCAGCAGCT GGATGACGCC CAGGTGCAAA TGGAGGAGCT GTTCCACGAA
    CGCAAGATCA AGCTGGACAT CTTCCTGCAG CTGCGCATCT TTGAGCAGTA CACCATTGAG
    GTGACAGCAG AGCTCGACGC TTGGAATGAG GATTTGCTTC GGCAGATGAA TGACTTCAAC
    ACGGAGGATC TAACACTGGC GGAACAGCGG CTGCAGCGCC ACACAGAGCG GAAGCTAGCC
    ATGAACAACA TGACCTTTGA GGTCATCCAG CAAGGCCAGG ATCTGCACCA GTACATCATG
    GAGGTCCAGG CCTCAGGGAT TGAGTTAATC TGTGAAAAAG ACATTGATCT GGCAGCCCAG
    GTACAAGAGC TATTAGAGTT TCTCCATGAG AAACAGCATG AGTTGGAGCT CAACGCAGAG
    CAGACCCACA AGCGACTTGA GCAGTGCCTC CAACTGCGGC ACCTCCAGGC CGAGGTCAAA
    CAGGTCCTAG GATGGATCCG CAATGGGGAG TCAATGCTCA ATGCCAGCCT GGTCAACGCC
    AGCTCTTTGT CTGAAGCAGA GCAGCTACAG CGGGAGCACG AGCAGTTCCA ACTAGCCATC
    GAGTCCCTCT TTCATGCCAC TTCCTTGCAG AAGACACACC AGAGCGCCCT GCAGGTTCAG
    CAGAAAGCTG AGGCGCTGCT CCAGGCTGGT CACTATGATG CAGATGCCAT CCGGGAATGT
    GCTGAGAAGG TGGCCCTCCA CTGGCAGCAA CTCATGCTCA AGATGGAAGA CCGACTGAAA
    CTGGTCAATG CCTCAGTGGC CTTTTATAAA ACTTCTGAGC AGGTCTGTAG TGTCCTGGAG
    AGCTTAGAGC AAGAATACCG AAGAGATGAG GACTGGTGTG GTGGACGAGA TAAATTGGGG
    CCAGCAGCAG AGATCGACCA CGTCATTCCA CTCATCAGTA AACATTTGGA ACAAAAGGAA
    GCCTTTCTTA AGGCCTGCAC CCTGGCACGG CGGAATGCTG AGGTCTTTCT CAAGTACATC
    CATAGGAACA ACGTCAGCAT GCCCAGTGCT GCCAGCCACA CCCGAGGACC CGAACAGCAA
    GTGAAAGCCA TCCTGAGTGA GCTCTTACAG AGGGAGAACC GTGTCCTGCA CTTCTGGACA
    TTGAAGAAGC GGCGGTTAGA CCAGTGTCAG CAATACGTGG TATTTGAGCG AAGCGCCAAG
    CAGGCACTAG ACTGGATCCA AGAAACAGGT GAATATTACC TCTCAACACA TACCTCCACC
    GGGGAGACCA CAGAGGAGAC TCAGGAACTG CTGAAAGAAT ATGGGGAGTT CAGGGTGCCT
    GCCAAGCAAA CAAAGGAGAA GGTGAAGCTT CTGATTCAGC TGGCTGATAG CTTTGTGGAA
    AAAGGTCACA TTCATGCCAC AGAGATCAGG AAATGGGTGA CCACAGTGGA CAAGCATTAC
    AGAGACTTCT CACTGAGGAT GGGAAAATAC CGGTACTCCC TGGAGAAGGC CCTAGGAGTC
    AACACGGAGG ATAACAAGGA CCTGGAGCTG GACATCATCC CAGCAAGCCT TTCAGATCGT
    GAGGTCAAGC TCCGGGATGC CAACCACGAG GTCAATGAAG AGAAGCGGAA ATCAGCCAGG
    AAAAAAGAAT TCATTATGGC AGAATTGCTC CAGACAGAGA AGGCTTATGT GAGGGACTTA
    CATGAGTGCT TAGAGACCTA CCTGTGGGAA ATGACCAGTG GTGTGGAGGA GATCCCCCCT
    GGGATCCTTA ATAAGGAGCA TATCATCTTT GGCAACATCC AAGAGATCTA CGATTTTCAT
    AACAACATCT TCCTCAAAGA ACTGGAGAAG TATGAGCAGC TGCCTGAGGA TGTGGGACAC
    TGCTTTGTTA CTTGGGCAGA CAAATTTCAG ATGTACGTCA CCTACTGTAA AAACAAGCCT
    GATTCCAACC AGCTGATCCT GGAGCATGCA GGCACCTTCT TTGATGAGAT ACAGCAGCGG
    CATGGTCTGG CCAACTCCAT TTCTTCCTAC CTAATTAAGC CTGTCCAGAG GATTACCAAG
    TACCAGCTGC TCCTGAAGGA ACTTTTAACT TGCTGTGAAG AAGGAAAAGG GGAGCTCAAG
    GAAGGCCTGG AGGTGATGCT TAGTGTCCCA AAGAGAGCCA ACGATGCCAT GCACGTCAGC
    ATGCTGGAAG GGTTCGACGA GAACCTGGAC GTGCAGGGGG AGCTGATTTT ACAAGATGCC
    TTTCAAGTGT GGGACCCCAA GTCGCTGATC CGGAAAGGGC GGGAGCGACA CCTATTCCTC
    TTTGAGATCT CCTTGGTTTT CAGCAAGGAG ATCAAAGACT CTTCAGGACA CACAAAATAT
    GTTTACAAGA ACAAGCTACT GACCTCAGAG CTGGGTGTGA CCGAACACGT AGAGGGCGAT
    CCCTGCAAAT TCGCCTTGTG GTCTGGGCGC ACCCCATCCT CAGACAATAA AACAGTGCTG
    AAAGCCTCCA ACATAGAAAC CAAGCAGGAG TGGATCAAGA ACATTCGAGA GGTGATTCAA
    GAAAGGATCA TTCACCTGAA AGGAGCTTTG AAGGAGCCAA TTCAGCTCCC CAAAACACCA
    GCCAAACAGA GGAACAACAG TAAGAGGGAT GGAGTGGAGG ATGTTGACAG CCAGGGGGAT
    GGGAGCAGCC AGCCAGACAC CATCTCCATT GCCTCTAGGA CCTCCCAGAA CACAGCGGAC
    AGTGACAAGC TCTCTGGCGG CTGTGAGTTG ACCGTGGTGC TCCAGGACTT CAACGCAGGC
    CACAGCAGCG AGCTGAGCAT CCAGGTGGGA CAGACGGTGG AGCTGCTAGA GCGGCCGAGC
    GAGCGGCCGG GCTGGTGTCT GGTCCGAACC ACAGAGCGGA GCCCCCCCCA GGAGGGCCTG
    GTGCCCAGCA GCGCCCTGTG CATCTCCCAC TCCCGAAGCA GCGTGGAGAT GGACTGCTTC
    TTCCCCCTGG TGAAAGATGC GTACTCTCAT TCCTCAAGTG AAAACGGAGG CAAGTCCGAG
    TCGGTGGCCA ACCTGCAGGC CCAACCATCC CTGAACTCCA TCCACAGCTC CCCTGGGCCC
    AAGCGTTCCA CCAACACCCT GAAGAAGTGG CTGACGAGTC CCGTGCGCCG GCTTAACAGT
    GGGAAAGCAG ATGGAAACAT CAAGAAGCAG AAGAAAGTAC GAGATGGTCG GAAGAGCTTT
    GACCTGGGAT CTCCCAAGCC TGGGGATGAG ACGACCCCTC AGGGAGACAG CGCTGATGAG
    AAGAGCAAGA AAGGTTGGGG TGAAGATGAG GCAGATGAAG AGTCACACAC ACCCCTCCCC
    CCGCCTATGA AGATCTTTGA CAACGACCCC ACGCAGGACG AAATGTCCTC CTCTTTGCTA
    GCAGCCCGGC AGGCTTCCAC CGAAGTACCT ACTGCTGCAG ACCTTGTCAG TGCGATAGAA
    CAGTTGGTCA AAAGCAAGCT GAGTCTGGAA GGAGGCTCTT ACCGAGGGAG CTTGAAAGAC
    CCCACAGGCT GCCTGAATGA AGGCATGACC CCACCCACAC CTCCCAGAAA TCTGGAAGAA
    GAACAGAAAG CCAAGGCCCT GAGAGGCAGG ATGTTTGTCC TGAATGAACT GGTACAAACA
    GAGAAGGACT ATGTCAAGGA CCTGGGAATT GTGGTGGAGG GTTTCATGAA GAGAATAGAA
    GAAAAGGGTG TCCCCGAGGA CATGCGAGGA AAGGATAAAA TCGTGTTTGG AAATATTCAC
    CAGATTTATG ATTGGCATAA GGATGTTTTC CTGGGTGAAC TGGAAAAATG TATCCAGGAG
    CAAGACAGAT TGGCACAGCT CTTTATTAAA TATGAGCGGA AGCTGCACAT CTATGTGTGG
    TACTGCCAGA ACAAGCCGCG CTCCGAGTAC ATCGTCGCCG AGTACGACGC CTACTTTGAA
    GAGGTGAAAC AAGAGATAAA TCAAAGGCTG ACGCTTAGCG ACTTCCTTAT CAAGCCCATT
    CAGAGAATAA CAAAATATCA ATTGCTCCTC AAGGACTTCC TGAGATACAG CGAGAAGGCT
    GGTTTGGAGT GTTCAGATAT TGAGAAAGCA GTGGAGTTAA TGTGCCTTGT TCCAAAACGC
    TGCAATGACA TGATGAACCT GGGGCGCCTG CAGGGCTTTG AGGGGACCCT GACTGCTCAG
    GGCAAGCTGC TGCAGCAGGA CACATTCTAC GTGATTGAGC TGGACGCTGG CATGCAGTCG
    CGGACCAAGG AGAGGCGTGT GTTTCTCTTT GAGCAAATCG TCATCTTCAG TGAACTGCTC
    AGGAAGGGAT CCCTCACCCC AGGCTACATG TTCAAAAGGA GCATCAAGAT GAACTACTTG
    GTCCTGGAGG AGAACGTGGA CAATGACCCC TGCAAGTTTG CGCTTCTGAA CAGGGAGACT
    TCAGAGAGGG TTATTCTGCA AGCCGCCAAT GCTGACATCC AGCAGGCCTG GGTGCAGGAC
    ATCAACCAAG TCTTAGAAAC ACAGCGAGAC TTCTTAAATG CCCTACAGTC GCCCATTGAG
    TATCAGCGGA AAGAAAGGAG CACAGCCGTG ATGAGGTCCC AGCCTGCTAG GGTTCCCCAA
    GCCAGCCCCA GGCCCTACTC CTCCATCCCC GTGGGCTCCG AGAAGCCCCC AAAGGGCTCC
    AGCTATAACC CGCCTCTGCC ACCCCTGAAG ATATCTACCT CCAATGGCAG TCCAGGGTTT
    GACTACCACC AGGCTGGGGA CAAGTTTGAG GCCAGCAAGA ATGACCTGGG AGGCTGCAAC
    GGGGCCTCAT CCATGGCCGT GATCAAAGAT TATTATGCAC TGAAGGAGAA CGAAATCTGT
    GTGAGCCAAG GTGAGGTGGT CCAGGTCCTC GCCGTCAACC AGCAGAACAT GTGCCTGGTG
    TACCAGCCCG CTAGCGACCA CTCCCCCGCC GCTGAGGGCT GGGTCCCTGG CAGCATCCTG
    ACGCCCCTCA CCAAAGCCAT GGCAGCCACA GAAAATAGTG ACGGGAGCAT CAAGAAGTCA
    TGTTCATGGC ATACTCTACG CATGAGAAAG CGGGCAGAAG TGGAGAACAC GGGTAAAAAT
    GAAGCCACAG GGCCTCGTAA ACCCAAGGAT ATTCTGGGCA ACAAAGTCTC TGTTAAAGAG
    ACGAACAGTT CCGAGGAGTC AGAGTGTGAT GATCTTGACC CTAATACTAG CATGGAGATC
    TTAAATCCAA ATTTCATCCA AGAAGTGGCG CCAGAATTCC TCGTGCCCTT AGTGGATGTG
    ACCTGCTTGC TTGGGGACAC AGTGACTCTG CAGTGCAAAG TCTGTGGGCG GCCAAAGCCC
    ACCATCACTT GGAAGGGTCC AGACCAGAAC ATCCTTGACA CTGACAACAG CTCAGCTACA
    TACACCGTCT CCTCCTGTGA CTCTGGGGAA ATCACCCTGA AAATCTGCAA TCTGATGCCC
    CAAGACAGCG GTATCTATAC CTGCATAGCA GCAAACGACC ATGGTACTGC GTCAACATCT
    GCTACTGTTA AAGTGCAAGG TGTTCCAGCG GCCCCTAACC GCCCCATTGC CCAAGAGAGA
    AGCTGCACTT CCGTGATCCT CCGCTGGCTG CCCCCATCCA GCACGGGAAA CTGCACCATT
    TCCGGTTACA CCGTGGAGTA CAGAGAGGAA GGTTCCCAGG CCTGGCAGCA GTCAGTAGCA
    TCGACCTTGG ACACCTACCT TGTCATTGAA GACCTCAGTC CTGGGAGTCC TTATCAGTTC
    AGAGTCAGTG CCAGTAACCC CTGGGGGATC AGCCTGCCCA GCGAGCCCTC AGAATTCGTG
    CGACTTCCAG ATTACGACGC TGCTGCCGAT GGTGCCACCA TTTCTTGGAA GGAAAATTTT
    GATTCAGCTT ATACTGAGCT GAATGAAATT GGAAGAGGCC GGTTCTCCAT AGTAAAGAAG
    TGCATTCACA AAGCTACTCG CAAAGATGTC GCTGTGAAAT TTGTTAGCAA AAAAATGAAG
    AAAAAAGAGC AGGCTGCCCA CGAGGCTGCC CTGCTTCAGC ACCTGCAGCA CCCCCAATAC
    ATCACTCTCC ATGACACCTA TGAGTCCCCC ACTTCCTACA TCCTCATTTT GGAACTGATG
    GACGATGGCC GACTCTTAGA CTATCTTATG AATCATGATG AGCTGATGGA AGAAAAAGTA
    GCTTTCTACA TTCGAGACAT CATGGAAGCC CTGCAGTACC TTCATAACTG CAGAGTTGCA
    CATTTGGACA TAAAGCCTGA AAACCTGCTC ATTGACCTAC GGATTCCAGT GCCTCGAGTG
    AAGCTCATTG ACTTGGAGGA TGCTGTCCAG ATCTCAGGTC ACTTCCATAT CCACCACCTT
    CTGGGGAACC CCGAGTTTGC TGCCCCAGAA GTGATCCAGG GCATCCCTGT ATCCCTGGGC
    ACCGACATCT GGAGCATCGG GGTTTTGACA TACGTCATGC TGAGTGGGGT CTCCCCCTTC
    TTGGATGAGA GCAAGGAGGA GACATGTATC AATGTGTGCA GGGTGGACTT CAGCTTCCCC
    CATGAGTACT TCTGCGGTGT GAGCAATGCT GCCAGAGATT TCATCAATGT GATCCTACAG
    GAAGACTTTC GGAGGCGGCC CACAGCAGCC ACCTGCCTGC AGCATCCGTG GCTGCAGCCC
    CACAATGGCA GCTACTCCAA GATCCCCCTG GACACCTCCC GCTTGGCGTG CTTCATCGAG
    CGCCGCAAGC ATCAGAATGA TGTGCGCCCC ATTCCCAACG TCAAGAGCTA CATTGTCAAT
    CGGCTAAACC AAGGCACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178293.1
    CDS1..8961
    Translation

    Target ORF information:

    RefSeq Version NM_001191364.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin, RhoGEF kinase (KALRN), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191364.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGACGGACC GCTTCTGGGA ACAGTGGTAT CTTTGGTATC TCCGCTTGCT CCGGCTGCTG 
    GATCGAGGCT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC CATCCTAAAG
    GAAAAAGTGG CCTTCGTGTC TGGGGGCCGT GATAAGCGAG GTGGACCCAT CCTGACCTTC
    CCTGCTCGCA GCAATCATGA CAGAATAAGA CAGGAAGACC TGCGGAAACT GGTGACCTAT
    CTGGCCAGCG TGCCAAGCGA GGACGTGTGC AAACGTGGCT TCACTGTCAT CATCGATATG
    CGGGGGTCCA AGTGGGACCT CATCAAGCCC CTCCTCAAGA CGCTGCAAGA AGCCTTTCCC
    GCTGAGATCC ATGTGGCCCT CATCATCAAA CCCGACAACT TCTGGCAGAA ACAGAAGACC
    AACTTTGGCA GCTCCAAGTT CATCTTTGAG ACAAGCATGG TGTCCGTGGA GGGCCTCACG
    AAGCTGGTGG ACCCCTCCCA GCTGACGGAG GAGTTCGATG GCTCCCTGGA CTACAACCAC
    GAGGAGTGGA TCGAGCTGCG GCTCTCCCTG GAGGAGTTCT TCAACAGCGC CGTACACCTG
    CTCTCACGCC TTGAAGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC CGTGGATGTG
    GAGGGCTCGA GGCGGCTAAT TGATGAACAC ACACAGCTGA AGAAGAAGGT GCTGAAGGCG
    CCTGTGGAGG AGCTGGACCG GGAGGGGCAG CGACTGCTGC AGTGCATCCG CTGCAGTGAC
    GGCTTCTCAG GGCGCAACTG CATCCCGGGC AGTGCCGACT TCCAGAGCTT GGTGCCCAAG
    ATCACCAGCC TGTTGGACAA GCTGCACTCC ACCCGGCAGC ACCTGCACCA GATGTGGCAT
    GTGCGCAAGC TCAAGCTAGA CCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAA
    AAGATGTTTG ACTGGATAAG CCACAACAAG GAGTTATTCC TCCAGAGCCA TACAGAGATT
    GGAGTCAGCT ACCAGCACGC CCTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC
    TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TGGCTTCCCG CCTCTCAGAG
    GCTGGCCACT ATGCTTCACA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG
    AAGAGTTTCG CAGCTGCCCT GGATGAACGC AGCACCATCC TCGCCATGTC TGCTGTGTTC
    CATCAGAAGG CTGAGCAGTT CTTGTCAGGA GTGGATGCCT GGTGCAAGAT GTGCAGTGAA
    GGTGGCCTGC CCTCTGAGAT GCAGGACCTG GAGCTGGCGA TCCACCACCA CCAGTCTTTG
    TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ACGGCAAAGC ACTGCTGGAT
    GTGCTGCAGC GGCCCCTGAG TCCCGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC
    TCCAAGGCGG TGCACCAGGT GCTGGACGTG GTGCACGAAG TGCTGCACCA CCAGCGGCGG
    CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTCCACC AACGCCTGCA GCTCTGCGTC
    TTCCAGCAGG ACGTCCAGCA GGTGTTAGAT TGGATTGAAA ACCACGGCGA GGCCTTTCTC
    AGCAAGCACA CAGGGGTCGG GAAGTCTCTG CACCGAGCTC GGGCCCTGCA GAAGAGGCAC
    GATGACTTTG AAGAGGTGGC TCAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCG
    GCAGAGCAGC TGGCTCAGAC AGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCTAGA
    CACCTGGAGG TGCGCATCCA AGACTTCGTG CGCAGGGTGG AGCAGCGGAA GCTTCTGCTG
    GACATGTCTG TCTCCTTCCA TACTCACACC AAAGAGCTAT GGACATGGAT GGAAGACCTT
    CAGAAGGAGA TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAACTGATC
    AAGCAGTTCC AGCAGCAGCA GACCGCCACT CTGGATGCCA CACTTAATGT CATCAAGGAG
    GGCGAAGACC TTATCCAGCA ACTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCAGCGAG
    GCCAGGGACT CAGCTGTGTC CAACAACAAG ACGCCCCATA GTAGCTCCAT CAGCCACATA
    GAGTCGGTGC TGCAGCAGCT GGATGACGCC CAGGTGCAAA TGGAGGAGCT GTTCCACGAA
    CGCAAGATCA AGCTGGACAT CTTCCTGCAG CTGCGCATCT TTGAGCAGTA CACCATTGAG
    GTGACAGCAG AGCTCGACGC TTGGAATGAG GATTTGCTTC GGCAGATGAA TGACTTCAAC
    ACGGAGGATC TAACACTGGC GGAACAGCGG CTGCAGCGCC ACACAGAGCG GAAGCTAGCC
    ATGAACAACA TGACCTTTGA GGTCATCCAG CAAGGCCAGG ATCTGCACCA GTACATCATG
    GAGGTCCAGG CCTCAGGGAT TGAGTTAATC TGTGAAAAAG ACATTGATCT GGCAGCCCAG
    GTACAAGAGC TATTAGAGTT TCTCCATGAG AAACAGCATG AGTTGGAGCT CAACGCAGAG
    CAGACCCACA AGCGACTTGA GCAGTGCCTC CAACTGCGGC ACCTCCAGGC CGAGGTCAAA
    CAGGTCCTAG GATGGATCCG CAATGGGGAG TCAATGCTCA ATGCCAGCCT GGTCAACGCC
    AGCTCTTTGT CTGAAGCAGA GCAGCTACAG CGGGAGCACG AGCAGTTCCA ACTAGCCATC
    GAGTCCCTCT TTCATGCCAC TTCCTTGCAG AAGACACACC AGAGCGCCCT GCAGGTTCAG
    CAGAAAGCTG AGGCGCTGCT CCAGGCTGGT CACTATGATG CAGATGCCAT CCGGGAATGT
    GCTGAGAAGG TGGCCCTCCA CTGGCAGCAA CTCATGCTCA AGATGGAAGA CCGACTGAAA
    CTGGTCAATG CCTCAGTGGC CTTTTATAAA ACTTCTGAGC AGGTCTGTAG TGTCCTGGAG
    AGCTTAGAGC AAGAATACCG AAGAGATGAG GACTGGTGTG GTGGACGAGA TAAATTGGGG
    CCAGCAGCAG AGATCGACCA CGTCATTCCA CTCATCAGTA AACATTTGGA ACAAAAGGAA
    GCCTTTCTTA AGGCCTGCAC CCTGGCACGG CGGAATGCTG AGGTCTTTCT CAAGTACATC
    CATAGGAACA ACGTCAGCAT GCCCAGTGCT GCCAGCCACA CCCGAGGACC CGAACAGCAA
    GTGAAAGCCA TCCTGAGTGA GCTCTTACAG AGGGAGAACC GTGTCCTGCA CTTCTGGACA
    TTGAAGAAGC GGCGGTTAGA CCAGTGTCAG CAATACGTGG TATTTGAGCG AAGCGCCAAG
    CAGGCACTAG ACTGGATCCA AGAAACAGGT GAATATTACC TCTCAACACA TACCTCCACC
    GGGGAGACCA CAGAGGAGAC TCAGGAACTG CTGAAAGAAT ATGGGGAGTT CAGGGTGCCT
    GCCAAGCAAA CAAAGGAGAA GGTGAAGCTT CTGATTCAGC TGGCTGATAG CTTTGTGGAA
    AAAGGTCACA TTCATGCCAC AGAGATCAGG AAATGGGTGA CCACAGTGGA CAAGCATTAC
    AGAGACTTCT CACTGAGGAT GGGAAAATAC CGGTACTCCC TGGAGAAGGC CCTAGGAGTC
    AACACGGAGG ATAACAAGGA CCTGGAGCTG GACATCATCC CAGCAAGCCT TTCAGATCGT
    GAGGTCAAGC TCCGGGATGC CAACCACGAG GTCAATGAAG AGAAGCGGAA ATCAGCCAGG
    AAAAAAGAAT TCATTATGGC AGAATTGCTC CAGACAGAGA AGGCTTATGT GAGGGACTTA
    CATGAGTGCT TAGAGACCTA CCTGTGGGAA ATGACCAGTG GTGTGGAGGA GATCCCCCCT
    GGGATCCTTA ATAAGGAGCA TATCATCTTT GGCAACATCC AAGAGATCTA CGATTTTCAT
    AACAACATCT TCCTCAAAGA ACTGGAGAAG TATGAGCAGC TGCCTGAGGA TGTGGGACAC
    TGCTTTGTTA CTTGGGCAGA CAAATTTCAG ATGTACGTCA CCTACTGTAA AAACAAGCCT
    GATTCCAACC AGCTGATCCT GGAGCATGCA GGCACCTTCT TTGATGAGAT ACAGCAGCGG
    CATGGTCTGG CCAACTCCAT TTCTTCCTAC CTAATTAAGC CTGTCCAGAG GATTACCAAG
    TACCAGCTGC TCCTGAAGGA ACTTTTAACT TGCTGTGAAG AAGGAAAAGG GGAGCTCAAG
    GAAGGCCTGG AGGTGATGCT TAGTGTCCCA AAGAGAGCCA ACGATGCCAT GCACGTCAGC
    ATGCTGGAAG GGTTCGACGA GAACCTGGAC GTGCAGGGGG AGCTGATTTT ACAAGATGCC
    TTTCAAGTGT GGGACCCCAA GTCGCTGATC CGGAAAGGGC GGGAGCGACA CCTATTCCTC
    TTTGAGATCT CCTTGGTTTT CAGCAAGGAG ATCAAAGACT CTTCAGGACA CACAAAATAT
    GTTTACAAGA ACAAGCTACT GACCTCAGAG CTGGGTGTGA CCGAACACGT AGAGGGCGAT
    CCCTGCAAAT TCGCCTTGTG GTCTGGGCGC ACCCCATCCT CAGACAATAA AACAGTGCTG
    AAAGCCTCCA ACATAGAAAC CAAGCAGGAG TGGATCAAGA ACATTCGAGA GGTGATTCAA
    GAAAGGATCA TTCACCTGAA AGGAGCTTTG AAGGAGCCAA TTCAGCTCCC CAAAACACCA
    GCCAAACAGA GGAACAACAG TAAGAGGGAT GGAGTGGAGG ATGTTGACAG CCAGGGGGAT
    GGGAGCAGCC AGCCAGACAC CATCTCCATT GCCTCTAGGA CCTCCCAGAA CACAGCGGAC
    AGTGACAAGC TCTCTGGCGG CTGTGAGTTG ACCGTGGTGC TCCAGGACTT CAACGCAGGC
    CACAGCAGCG AGCTGAGCAT CCAGGTGGGA CAGACGGTGG AGCTGCTAGA GCGGCCGAGC
    GAGCGGCCGG GCTGGTGTCT GGTCCGAACC ACAGAGCGGA GCCCCCCCCA GGAGGGCCTG
    GTGCCCAGCA GCGCCCTGTG CATCTCCCAC TCCCGAAGCA GCGTGGAGAT GGACTGCTTC
    TTCCCCCTGG TGAAAGATGC GTACTCTCAT TCCTCAAGTG AAAACGGAGG CAAGTCCGAG
    TCGGTGGCCA ACCTGCAGGC CCAACCATCC CTGAACTCCA TCCACAGCTC CCCTGGGCCC
    AAGCGTTCCA CCAACACCCT GAAGAAGTGG CTGACGAGTC CCGTGCGCCG GCTTAACAGT
    GGGAAAGCAG ATGGAAACAT CAAGAAGCAG AAGAAAGTAC GAGATGGTCG GAAGAGCTTT
    GACCTGGGAT CTCCCAAGCC TGGGGATGAG ACGACCCCTC AGGGAGACAG CGCTGATGAG
    AAGAGCAAGA AAGGTTGGGG TGAAGATGAG GCAGATGAAG AGTCACACAC ACCCCTCCCC
    CCGCCTATGA AGATCTTTGA CAACGACCCC ACGCAGGACG AAATGTCCTC CTCTTTGCTA
    GCAGCCCGGC AGGCTTCCAC CGAAGTACCT ACTGCTGCAG ACCTTGTCAG TGCGATAGAA
    CAGTTGGTCA AAAGCAAGCT GAGTCTGGAA GGAGGCTCTT ACCGAGGGAG CTTGAAAGAC
    CCCACAGGCT GCCTGAATGA AGGCATGACC CCACCCACAC CTCCCAGAAA TCTGGAAGAA
    GAACAGAAAG CCAAGGCCCT GAGAGGCAGG ATGTTTGTCC TGAATGAACT GGTACAAACA
    GAGAAGGACT ATGTCAAGGA CCTGGGAATT GTGGTGGAGG GTTTCATGAA GAGAATAGAA
    GAAAAGGGTG TCCCCGAGGA CATGCGAGGA AAGGATAAAA TCGTGTTTGG AAATATTCAC
    CAGATTTATG ATTGGCATAA GGATGTTTTC CTGGGTGAAC TGGAAAAATG TATCCAGGAG
    CAAGACAGAT TGGCACAGCT CTTTATTAAA TATGAGCGGA AGCTGCACAT CTATGTGTGG
    TACTGCCAGA ACAAGCCGCG CTCCGAGTAC ATCGTCGCCG AGTACGACGC CTACTTTGAA
    GAGGTGAAAC AAGAGATAAA TCAAAGGCTG ACGCTTAGCG ACTTCCTTAT CAAGCCCATT
    CAGAGAATAA CAAAATATCA ATTGCTCCTC AAGGACTTCC TGAGATACAG CGAGAAGGCT
    GGTTTGGAGT GTTCAGATAT TGAGAAAGCA GTGGAGTTAA TGTGCCTTGT TCCAAAACGC
    TGCAATGACA TGATGAACCT GGGGCGCCTG CAGGGCTTTG AGGGGACCCT GACTGCTCAG
    GGCAAGCTGC TGCAGCAGGA CACATTCTAC GTGATTGAGC TGGACGCTGG CATGCAGTCG
    CGGACCAAGG AGAGGCGTGT GTTTCTCTTT GAGCAAATCG TCATCTTCAG TGAACTGCTC
    AGGAAGGGAT CCCTCACCCC AGGCTACATG TTCAAAAGGA GCATCAAGAT GAACTACTTG
    GTCCTGGAGG AGAACGTGGA CAATGACCCC TGCAAGTTTG CGCTTCTGAA CAGGGAGACT
    TCAGAGAGGG TTATTCTGCA AGCCGCCAAT GCTGACATCC AGCAGGCCTG GGTGCAGGAC
    ATCAACCAAG TCTTAGAAAC ACAGCGAGAC TTCTTAAATG CCCTACAGTC GCCCATTGAG
    TATCAGCGGA AAGAAAGGAG CACAGCCGTG ATGAGGTCCC AGCCTGCTAG GGTTCCCCAA
    GCCAGCCCCA GGCCCTACTC CTCCATCCCC GTGGGCTCCG AGAAGCCCCC AAAGGGCTCC
    AGCTATAACC CGCCTCTGCC ACCCCTGAAG ATATCTACCT CCAATGGCAG TCCAGGGTTT
    GACTACCACC AGGCTGGGGA CAAGTTTGAG GCCAGCAAGA ATGACCTGGG AGGCTGCAAC
    GGGGCCTCAT CCATGGCCGT GATCAAAGAT TATTATGCAC TGAAGGAGAA CGAAATCTGT
    GTGAGCCAAG GTGAGGTGGT CCAGGTCCTC GCCGTCAACC AGCAGAACAT GTGCCTGGTG
    TACCAGCCCG CTAGCGACCA CTCCCCCGCC GCTGAGGGCT GGGTCCCTGG CAGCATCCTG
    ACGCCCCTCA CCAAAGCCAT GGCAGCCACA GAAAATAGTG ACGGGAGCAT CAAGAAGTCA
    TGTTCATGGC ATACTCTACG CATGAGAAAG CGGGCAGAAG TGGAGAACAC GGGTAAAAAT
    GAAGCCACAG GGCCTCGTAA ACCCAAGGAT ATTCTGGGCA ACAAAGTCTC TGTTAAAGAG
    ACGAACAGTT CCGAGGAGTC AGAGTGTGAT GATCTTGACC CTAATACTAG CATGGAGATC
    TTAAATCCAA ATTTCATCCA AGAAGTGGCG CCAGAATTCC TCGTGCCCTT AGTGGATGTG
    ACCTGCTTGC TTGGGGACAC AGTGACTCTG CAGTGCAAAG TCTGTGGGCG GCCAAAGCCC
    ACCATCACTT GGAAGGGTCC AGACCAGAAC ATCCTTGACA CTGACAACAG CTCAGCTACA
    TACACCGTCT CCTCCTGTGA CTCTGGGGAA ATCACCCTGA AAATCTGCAA TCTGATGCCC
    CAAGACAGCG GTATCTATAC CTGCATAGCA GCAAACGACC ATGGTACTGC GTCAACATCT
    GCTACTGTTA AAGTGCAAGG TGTTCCAGCG GCCCCTAACC GCCCCATTGC CCAAGAGAGA
    AGCTGCACTT CCGTGATCCT CCGCTGGCTG CCCCCATCCA GCACGGGAAA CTGCACCATT
    TCCGGTTACA CCGTGGAGTA CAGAGAGGAA GGTTCCCAGG CCTGGCAGCA GTCAGTAGCA
    TCGACCTTGG ACACCTACCT TGTCATTGAA GACCTCAGTC CTGGGAGTCC TTATCAGTTC
    AGAGTCAGTG CCAGTAACCC CTGGGGGATC AGCCTGCCCA GCGAGCCCTC AGAATTCGTG
    CGACTTCCAG ATTACGACGC TGCTGCCGAT GGTGCCACCA TTTCTTGGAA GGAAAATTTT
    GATTCAGCTT ATACTGAGCT GAATGAAATT GGAAGAGGCC GGTTCTCCAT AGTAAAGAAG
    TGCATTCACA AAGCTACTCG CAAAGATGTC GCTGTGAAAT TTGTTAGCAA AAAAATGAAG
    AAAAAAGAGC AGGCTGCCCA CGAGGCTGCC CTGCTTCAGC ACCTGCAGCA CCCCCAATAC
    ATCACTCTCC ATGACACCTA TGAGTCCCCC ACTTCCTACA TCCTCATTTT GGAACTGATG
    GACGATGGCC GACTCTTAGA CTATCTTATG AATCATGATG AGCTGATGGA AGAAAAAGTA
    GCTTTCTACA TTCGAGACAT CATGGAAGCC CTGCAGTACC TTCATAACTG CAGAGTTGCA
    CATTTGGACA TAAAGCCTGA AAACCTGCTC ATTGACCTAC GGATTCCAGT GCCTCGAGTG
    AAGCTCATTG ACTTGGAGGA TGCTGTCCAG ATCTCAGGTC ACTTCCATAT CCACCACCTT
    CTGGGGAACC CCGAGTTTGC TGCCCCAGAA GTGATCCAGG GCATCCCTGT ATCCCTGGGC
    ACCGACATCT GGAGCATCGG GGTTTTGACA TACGTCATGC TGAGTGGGGT CTCCCCCTTC
    TTGGATGAGA GCAAGGAGGA GACATGTATC AATGTGTGCA GGGTGGACTT CAGCTTCCCC
    CATGAGTACT TCTGCGGTGT GAGCAATGCT GCCAGAGATT TCATCAATGT GATCCTACAG
    GAAGACTTTC GGAGGCGGCC CACAGCAGCC ACCTGCCTGC AGCATCCGTG GCTGCAGCCC
    CACAATGGCA GCTACTCCAA GATCCCCCTG GACACCTCCC GCTTGGCGTG CTTCATCGAG
    CGCCGCAAGC ATCAGAATGA TGTGCGCCCC ATTCCCAACG TCAAGAGCTA CATTGTCAAT
    CGGCTAAACC AAGGCACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218890
    Clone ID Related Accession (Same CDS sequence) XM_024991355.1
    Accession Version XM_024991355.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8967bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CACACCAGAG CGCCCTGCAG
    GTTCAGCAGA AAGCTGAGGC GCTGCTCCAG GCTGGTCACT ATGATGCAGA TGCCATCCGG
    GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAGCAACTCA TGCTCAAGAT GGAAGACCGA
    CTGAAACTGG TCAATGCCTC AGTGGCCTTT TATAAAACTT CTGAGCAGGT CTGTAGTGTC
    CTGGAGAGCT TAGAGCAAGA ATACCGAAGA GATGAGGACT GGTGTGGTGG ACGAGATAAA
    TTGGGGCCAG CAGCAGAGAT CGACCACGTC ATTCCACTCA TCAGTAAACA TTTGGAACAA
    AAGGAAGCCT TTCTTAAGGC CTGCACCCTG GCACGGCGGA ATGCTGAGGT CTTTCTCAAG
    TACATCCATA GGAACAACGT CAGCATGCCC AGTGCTGCCA GCCACACCCG AGGACCCGAA
    CAGCAAGTGA AAGCCATCCT GAGTGAGCTC TTACAGAGGG AGAACCGTGT CCTGCACTTC
    TGGACATTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ACGTGGTATT TGAGCGAAGC
    GCCAAGCAGG CACTAGACTG GATCCAAGAA ACAGGTGAAT ATTACCTCTC AACACATACC
    TCCACCGGGG AGACCACAGA GGAGACTCAG GAACTGCTGA AAGAATATGG GGAGTTCAGG
    GTGCCTGCCA AGCAAACAAA GGAGAAGGTG AAGCTTCTGA TTCAGCTGGC TGATAGCTTT
    GTGGAAAAAG GTCACATTCA TGCCACAGAG ATCAGGAAAT GGGTGACCAC AGTGGACAAG
    CATTACAGAG ACTTCTCACT GAGGATGGGA AAATACCGGT ACTCCCTGGA GAAGGCCCTA
    GGAGTCAACA CGGAGGATAA CAAGGACCTG GAGCTGGACA TCATCCCAGC AAGCCTTTCA
    GATCGTGAGG TCAAGCTCCG GGATGCCAAC CACGAGGTCA ATGAAGAGAA GCGGAAATCA
    GCCAGGAAAA AAGAATTCAT TATGGCAGAA TTGCTCCAGA CAGAGAAGGC TTATGTGAGG
    GACTTACATG AGTGCTTAGA GACCTACCTG TGGGAAATGA CCAGTGGTGT GGAGGAGATC
    CCCCCTGGGA TCCTTAATAA GGAGCATATC ATCTTTGGCA ACATCCAAGA GATCTACGAT
    TTTCATAACA ACATCTTCCT CAAAGAACTG GAGAAGTATG AGCAGCTGCC TGAGGATGTG
    GGACACTGCT TTGTTACTTG GGCAGACAAA TTTCAGATGT ACGTCACCTA CTGTAAAAAC
    AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCAGGCA CCTTCTTTGA TGAGATACAG
    CAGCGGCATG GTCTGGCCAA CTCCATTTCT TCCTACCTAA TTAAGCCTGT CCAGAGGATT
    ACCAAGTACC AGCTGCTCCT GAAGGAACTT TTAACTTGCT GTGAAGAAGG AAAAGGGGAG
    CTCAAGGAAG GCCTGGAGGT GATGCTTAGT GTCCCAAAGA GAGCCAACGA TGCCATGCAC
    GTCAGCATGC TGGAAGGGTT CGACGAGAAC CTGGACGTGC AGGGGGAGCT GATTTTACAA
    GATGCCTTTC AAGTGTGGGA CCCCAAGTCG CTGATCCGGA AAGGGCGGGA GCGACACCTA
    TTCCTCTTTG AGATCTCCTT GGTTTTCAGC AAGGAGATCA AAGACTCTTC AGGACACACA
    AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA ACACGTAGAG
    GGCGATCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA
    GTGCTGAAAG CCTCCAACAT AGAAACCAAG CAGGAGTGGA TCAAGAACAT TCGAGAGGTG
    ATTCAAGAAA GGATCATTCA CCTGAAAGGA GCTTTGAAGG AGCCAATTCA GCTCCCCAAA
    ACACCAGCCA AACAGAGGAA CAACAGTAAG AGGGATGGAG TGGAGGATGT TGACAGCCAG
    GGGGATGGGA GCAGCCAGCC AGACACCATC TCCATTGCCT CTAGGACCTC CCAGAACACA
    GCGGACAGTG ACAAGCTCTC TGGCGGCTGT GAGTTGACCG TGGTGCTCCA GGACTTCAAC
    GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGACAGA CGGTGGAGCT GCTAGAGCGG
    CCGAGCGAGC GGCCGGGCTG GTGTCTGGTC CGAACCACAG AGCGGAGCCC CCCCCAGGAG
    GGCCTGGTGC CCAGCAGCGC CCTGTGCATC TCCCACTCCC GAAGCAGCGT GGAGATGGAC
    TGCTTCTTCC CCCTGGTGAA AGATGCGTAC TCTCATTCCT CAAGTGAAAA CGGAGGCAAG
    TCCGAGTCGG TGGCCAACCT GCAGGCCCAA CCATCCCTGA ACTCCATCCA CAGCTCCCCT
    GGGCCCAAGC GTTCCACCAA CACCCTGAAG AAGTGGCTGA CGAGTCCCGT GCGCCGGCTT
    AACAGTGGGA AAGCAGATGG AAACATCAAG AAGCAGAAGA AAGTACGAGA TGGTCGGAAG
    AGCTTTGACC TGGGATCTCC CAAGCCTGGG GATGAGACGA CCCCTCAGGG AGACAGCGCT
    GATGAGAAGA GCAAGAAAGG TTGGGGTGAA GATGAGGCAG ATGAAGAGTC ACACACACCC
    CTCCCCCCGC CTATGAAGAT CTTTGACAAC GACCCCACGC AGGACGAAAT GTCCTCCTCT
    TTGCTAGCAG CCCGGCAGGC TTCCACCGAA GTACCTACTG CTGCAGACCT TGTCAGTGCG
    ATAGAACAGT TGGTCAAAAG CAAGCTGAGT CTGGAAGGAG GCTCTTACCG AGGGAGCTTG
    AAAGACCCCA CAGGCTGCCT GAATGAAGGC ATGACCCCAC CCACACCTCC CAGAAATCTG
    GAAGAAGAAC AGAAAGCCAA GGCCCTGAGA GGCAGGATGT TTGTCCTGAA TGAACTGGTA
    CAAACAGAGA AGGACTATGT CAAGGACCTG GGAATTGTGG TGGAGGGTTT CATGAAGAGA
    ATAGAAGAAA AGGGTGTCCC CGAGGACATG CGAGGAAAGG ATAAAATCGT GTTTGGAAAT
    ATTCACCAGA TTTATGATTG GCATAAGGAT GTTTTCCTGG GTGAACTGGA AAAATGTATC
    CAGGAGCAAG ACAGATTGGC ACAGCTCTTT ATTAAATATG AGCGGAAGCT GCACATCTAT
    GTGTGGTACT GCCAGAACAA GCCGCGCTCC GAGTACATCG TCGCCGAGTA CGACGCCTAC
    TTTGAAGAGG TGAAACAAGA GATAAATCAA AGGCTGACGC TTAGCGACTT CCTTATCAAG
    CCCATTCAGA GAATAACAAA ATATCAATTG CTCCTCAAGG ACTTCCTGAG ATACAGCGAG
    AAGGCTGGTT TGGAGTGTTC AGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTTCCA
    AAACGCTGCA ATGACATGAT GAACCTGGGG CGCCTGCAGG GCTTTGAGGG GACCCTGACT
    GCTCAGGGCA AGCTGCTGCA GCAGGACACA TTCTACGTGA TTGAGCTGGA CGCTGGCATG
    CAGTCGCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AAATCGTCAT CTTCAGTGAA
    CTGCTCAGGA AGGGATCCCT CACCCCAGGC TACATGTTCA AAAGGAGCAT CAAGATGAAC
    TACTTGGTCC TGGAGGAGAA CGTGGACAAT GACCCCTGCA AGTTTGCGCT TCTGAACAGG
    GAGACTTCAG AGAGGGTTAT TCTGCAAGCC GCCAATGCTG ACATCCAGCA GGCCTGGGTG
    CAGGACATCA ACCAAGTCTT AGAAACACAG CGAGACTTCT TAAATGCCCT ACAGTCGCCC
    ATTGAGTATC AGCGGAAAGA AAGGAGCACA GCCGTGATGA GGTCCCAGCC TGCTAGGGTT
    CCCCAAGCCA GCCCCAGGCC CTACTCCTCC ATCCCCGTGG GCTCCGAGAA GCCCCCAAAG
    GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCCAA TGGCAGTCCA
    GGGTTTGACT ACCACCAGGC TGGGGACAAG TTTGAGGCCA GCAAGAATGA CCTGGGAGGC
    TGCAACGGGG CCTCATCCAT GGCCGTGATC AAAGATTATT ATGCACTGAA GGAGAACGAA
    ATCTGTGTGA GCCAAGGTGA GGTGGTCCAG GTCCTCGCCG TCAACCAGCA GAACATGTGC
    CTGGTGTACC AGCCCGCTAG CGACCACTCC CCCGCCGCTG AGGGCTGGGT CCCTGGCAGC
    ATCCTGACGC CCCTCACCAA AGCCATGGCA GCCACAGAAA ATAGTGACGG GAGCATCAAG
    AAGTCATGTT CATGGCATAC TCTACGCATG AGAAAGCGGG CAGAAGTGGA GAACACGGGT
    AAAAATGAAG CCACAGGGCC TCGTAAACCC AAGGATATTC TGGGCAACAA AGTCTCTGTT
    AAAGAGACGA ACAGTTCCGA GGAGTCAGAG TGTGATGATC TTGACCCTAA TACTAGCATG
    GAGATCTTAA ATCCAAATTT CATCCAAGAA GTGGCGCCAG AATTCCTCGT GCCCTTAGTG
    GATGTGACCT GCTTGCTTGG GGACACAGTG ACTCTGCAGT GCAAAGTCTG TGGGCGGCCA
    AAGCCCACCA TCACTTGGAA GGGTCCAGAC CAGAACATCC TTGACACTGA CAACAGCTCA
    GCTACATACA CCGTCTCCTC CTGTGACTCT GGGGAAATCA CCCTGAAAAT CTGCAATCTG
    ATGCCCCAAG ACAGCGGTAT CTATACCTGC ATAGCAGCAA ACGACCATGG TACTGCGTCA
    ACATCTGCTA CTGTTAAAGT GCAAGGTGTT CCAGCGGCCC CTAACCGCCC CATTGCCCAA
    GAGAGAAGCT GCACTTCCGT GATCCTCCGC TGGCTGCCCC CATCCAGCAC GGGAAACTGC
    ACCATTTCCG GTTACACCGT GGAGTACAGA GAGGAAGGTT CCCAGGCCTG GCAGCAGTCA
    GTAGCATCGA CCTTGGACAC CTACCTTGTC ATTGAAGACC TCAGTCCTGG GAGTCCTTAT
    CAGTTCAGAG TCAGTGCCAG TAACCCCTGG GGGATCAGCC TGCCCAGCGA GCCCTCAGAA
    TTCGTGCGAC TTCCAGATTA CGACGCTGCT GCCGATGGTG CCACCATTTC TTGGAAGGAA
    AATTTTGATT CAGCTTATAC TGAGCTGAAT GAAATTGGAA GAGGCCGGTT CTCCATAGTA
    AAGAAGTGCA TTCACAAAGC TACTCGCAAA GATGTCGCTG TGAAATTTGT TAGCAAAAAA
    ATGAAGAAAA AAGAGCAGGC TGCCCACGAG GCTGCCCTGC TTCAGCACCT GCAGCACCCC
    CAATACATCA CTCTCCATGA CACCTATGAG TCCCCCACTT CCTACATCCT CATTTTGGAA
    CTGATGGACG ATGGCCGACT CTTAGACTAT CTTATGAATC ATGATGAGCT GATGGAAGAA
    AAAGTAGCTT TCTACATTCG AGACATCATG GAAGCCCTGC AGTACCTTCA TAACTGCAGA
    GTTGCACATT TGGACATAAA GCCTGAAAAC CTGCTCATTG ACCTACGGAT TCCAGTGCCT
    CGAGTGAAGC TCATTGACTT GGAGGATGCT GTCCAGATCT CAGGTCACTT CCATATCCAC
    CACCTTCTGG GGAACCCCGA GTTTGCTGCC CCAGAAGTGA TCCAGGGCAT CCCTGTATCC
    CTGGGCACCG ACATCTGGAG CATCGGGGTT TTGACATACG TCATGCTGAG TGGGGTCTCC
    CCCTTCTTGG ATGAGAGCAA GGAGGAGACA TGTATCAATG TGTGCAGGGT GGACTTCAGC
    TTCCCCCATG AGTACTTCTG CGGTGTGAGC AATGCTGCCA GAGATTTCAT CAATGTGATC
    CTACAGGAAG ACTTTCGGAG GCGGCCCACA GCAGCCACCT GCCTGCAGCA TCCGTGGCTG
    CAGCCCCACA ATGGCAGCTA CTCCAAGATC CCCCTGGACA CCTCCCGCTT GGCGTGCTTC
    ATCGAGCGCC GCAAGCATCA GAATGATGTG CGCCCCATTC CCAACGTCAA GAGCTACATT
    GTCAATCGGC TAAACCAAGG CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847123.1
    CDS360..9326
    Translation

    Target ORF information:

    RefSeq Version XM_024991355.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991355.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CACACCAGAG CGCCCTGCAG
    GTTCAGCAGA AAGCTGAGGC GCTGCTCCAG GCTGGTCACT ATGATGCAGA TGCCATCCGG
    GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAGCAACTCA TGCTCAAGAT GGAAGACCGA
    CTGAAACTGG TCAATGCCTC AGTGGCCTTT TATAAAACTT CTGAGCAGGT CTGTAGTGTC
    CTGGAGAGCT TAGAGCAAGA ATACCGAAGA GATGAGGACT GGTGTGGTGG ACGAGATAAA
    TTGGGGCCAG CAGCAGAGAT CGACCACGTC ATTCCACTCA TCAGTAAACA TTTGGAACAA
    AAGGAAGCCT TTCTTAAGGC CTGCACCCTG GCACGGCGGA ATGCTGAGGT CTTTCTCAAG
    TACATCCATA GGAACAACGT CAGCATGCCC AGTGCTGCCA GCCACACCCG AGGACCCGAA
    CAGCAAGTGA AAGCCATCCT GAGTGAGCTC TTACAGAGGG AGAACCGTGT CCTGCACTTC
    TGGACATTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ACGTGGTATT TGAGCGAAGC
    GCCAAGCAGG CACTAGACTG GATCCAAGAA ACAGGTGAAT ATTACCTCTC AACACATACC
    TCCACCGGGG AGACCACAGA GGAGACTCAG GAACTGCTGA AAGAATATGG GGAGTTCAGG
    GTGCCTGCCA AGCAAACAAA GGAGAAGGTG AAGCTTCTGA TTCAGCTGGC TGATAGCTTT
    GTGGAAAAAG GTCACATTCA TGCCACAGAG ATCAGGAAAT GGGTGACCAC AGTGGACAAG
    CATTACAGAG ACTTCTCACT GAGGATGGGA AAATACCGGT ACTCCCTGGA GAAGGCCCTA
    GGAGTCAACA CGGAGGATAA CAAGGACCTG GAGCTGGACA TCATCCCAGC AAGCCTTTCA
    GATCGTGAGG TCAAGCTCCG GGATGCCAAC CACGAGGTCA ATGAAGAGAA GCGGAAATCA
    GCCAGGAAAA AAGAATTCAT TATGGCAGAA TTGCTCCAGA CAGAGAAGGC TTATGTGAGG
    GACTTACATG AGTGCTTAGA GACCTACCTG TGGGAAATGA CCAGTGGTGT GGAGGAGATC
    CCCCCTGGGA TCCTTAATAA GGAGCATATC ATCTTTGGCA ACATCCAAGA GATCTACGAT
    TTTCATAACA ACATCTTCCT CAAAGAACTG GAGAAGTATG AGCAGCTGCC TGAGGATGTG
    GGACACTGCT TTGTTACTTG GGCAGACAAA TTTCAGATGT ACGTCACCTA CTGTAAAAAC
    AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCAGGCA CCTTCTTTGA TGAGATACAG
    CAGCGGCATG GTCTGGCCAA CTCCATTTCT TCCTACCTAA TTAAGCCTGT CCAGAGGATT
    ACCAAGTACC AGCTGCTCCT GAAGGAACTT TTAACTTGCT GTGAAGAAGG AAAAGGGGAG
    CTCAAGGAAG GCCTGGAGGT GATGCTTAGT GTCCCAAAGA GAGCCAACGA TGCCATGCAC
    GTCAGCATGC TGGAAGGGTT CGACGAGAAC CTGGACGTGC AGGGGGAGCT GATTTTACAA
    GATGCCTTTC AAGTGTGGGA CCCCAAGTCG CTGATCCGGA AAGGGCGGGA GCGACACCTA
    TTCCTCTTTG AGATCTCCTT GGTTTTCAGC AAGGAGATCA AAGACTCTTC AGGACACACA
    AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA ACACGTAGAG
    GGCGATCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA
    GTGCTGAAAG CCTCCAACAT AGAAACCAAG CAGGAGTGGA TCAAGAACAT TCGAGAGGTG
    ATTCAAGAAA GGATCATTCA CCTGAAAGGA GCTTTGAAGG AGCCAATTCA GCTCCCCAAA
    ACACCAGCCA AACAGAGGAA CAACAGTAAG AGGGATGGAG TGGAGGATGT TGACAGCCAG
    GGGGATGGGA GCAGCCAGCC AGACACCATC TCCATTGCCT CTAGGACCTC CCAGAACACA
    GCGGACAGTG ACAAGCTCTC TGGCGGCTGT GAGTTGACCG TGGTGCTCCA GGACTTCAAC
    GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGACAGA CGGTGGAGCT GCTAGAGCGG
    CCGAGCGAGC GGCCGGGCTG GTGTCTGGTC CGAACCACAG AGCGGAGCCC CCCCCAGGAG
    GGCCTGGTGC CCAGCAGCGC CCTGTGCATC TCCCACTCCC GAAGCAGCGT GGAGATGGAC
    TGCTTCTTCC CCCTGGTGAA AGATGCGTAC TCTCATTCCT CAAGTGAAAA CGGAGGCAAG
    TCCGAGTCGG TGGCCAACCT GCAGGCCCAA CCATCCCTGA ACTCCATCCA CAGCTCCCCT
    GGGCCCAAGC GTTCCACCAA CACCCTGAAG AAGTGGCTGA CGAGTCCCGT GCGCCGGCTT
    AACAGTGGGA AAGCAGATGG AAACATCAAG AAGCAGAAGA AAGTACGAGA TGGTCGGAAG
    AGCTTTGACC TGGGATCTCC CAAGCCTGGG GATGAGACGA CCCCTCAGGG AGACAGCGCT
    GATGAGAAGA GCAAGAAAGG TTGGGGTGAA GATGAGGCAG ATGAAGAGTC ACACACACCC
    CTCCCCCCGC CTATGAAGAT CTTTGACAAC GACCCCACGC AGGACGAAAT GTCCTCCTCT
    TTGCTAGCAG CCCGGCAGGC TTCCACCGAA GTACCTACTG CTGCAGACCT TGTCAGTGCG
    ATAGAACAGT TGGTCAAAAG CAAGCTGAGT CTGGAAGGAG GCTCTTACCG AGGGAGCTTG
    AAAGACCCCA CAGGCTGCCT GAATGAAGGC ATGACCCCAC CCACACCTCC CAGAAATCTG
    GAAGAAGAAC AGAAAGCCAA GGCCCTGAGA GGCAGGATGT TTGTCCTGAA TGAACTGGTA
    CAAACAGAGA AGGACTATGT CAAGGACCTG GGAATTGTGG TGGAGGGTTT CATGAAGAGA
    ATAGAAGAAA AGGGTGTCCC CGAGGACATG CGAGGAAAGG ATAAAATCGT GTTTGGAAAT
    ATTCACCAGA TTTATGATTG GCATAAGGAT GTTTTCCTGG GTGAACTGGA AAAATGTATC
    CAGGAGCAAG ACAGATTGGC ACAGCTCTTT ATTAAATATG AGCGGAAGCT GCACATCTAT
    GTGTGGTACT GCCAGAACAA GCCGCGCTCC GAGTACATCG TCGCCGAGTA CGACGCCTAC
    TTTGAAGAGG TGAAACAAGA GATAAATCAA AGGCTGACGC TTAGCGACTT CCTTATCAAG
    CCCATTCAGA GAATAACAAA ATATCAATTG CTCCTCAAGG ACTTCCTGAG ATACAGCGAG
    AAGGCTGGTT TGGAGTGTTC AGATATTGAG AAAGCAGTGG AGTTAATGTG CCTTGTTCCA
    AAACGCTGCA ATGACATGAT GAACCTGGGG CGCCTGCAGG GCTTTGAGGG GACCCTGACT
    GCTCAGGGCA AGCTGCTGCA GCAGGACACA TTCTACGTGA TTGAGCTGGA CGCTGGCATG
    CAGTCGCGGA CCAAGGAGAG GCGTGTGTTT CTCTTTGAGC AAATCGTCAT CTTCAGTGAA
    CTGCTCAGGA AGGGATCCCT CACCCCAGGC TACATGTTCA AAAGGAGCAT CAAGATGAAC
    TACTTGGTCC TGGAGGAGAA CGTGGACAAT GACCCCTGCA AGTTTGCGCT TCTGAACAGG
    GAGACTTCAG AGAGGGTTAT TCTGCAAGCC GCCAATGCTG ACATCCAGCA GGCCTGGGTG
    CAGGACATCA ACCAAGTCTT AGAAACACAG CGAGACTTCT TAAATGCCCT ACAGTCGCCC
    ATTGAGTATC AGCGGAAAGA AAGGAGCACA GCCGTGATGA GGTCCCAGCC TGCTAGGGTT
    CCCCAAGCCA GCCCCAGGCC CTACTCCTCC ATCCCCGTGG GCTCCGAGAA GCCCCCAAAG
    GGCTCCAGCT ATAACCCGCC TCTGCCACCC CTGAAGATAT CTACCTCCAA TGGCAGTCCA
    GGGTTTGACT ACCACCAGGC TGGGGACAAG TTTGAGGCCA GCAAGAATGA CCTGGGAGGC
    TGCAACGGGG CCTCATCCAT GGCCGTGATC AAAGATTATT ATGCACTGAA GGAGAACGAA
    ATCTGTGTGA GCCAAGGTGA GGTGGTCCAG GTCCTCGCCG TCAACCAGCA GAACATGTGC
    CTGGTGTACC AGCCCGCTAG CGACCACTCC CCCGCCGCTG AGGGCTGGGT CCCTGGCAGC
    ATCCTGACGC CCCTCACCAA AGCCATGGCA GCCACAGAAA ATAGTGACGG GAGCATCAAG
    AAGTCATGTT CATGGCATAC TCTACGCATG AGAAAGCGGG CAGAAGTGGA GAACACGGGT
    AAAAATGAAG CCACAGGGCC TCGTAAACCC AAGGATATTC TGGGCAACAA AGTCTCTGTT
    AAAGAGACGA ACAGTTCCGA GGAGTCAGAG TGTGATGATC TTGACCCTAA TACTAGCATG
    GAGATCTTAA ATCCAAATTT CATCCAAGAA GTGGCGCCAG AATTCCTCGT GCCCTTAGTG
    GATGTGACCT GCTTGCTTGG GGACACAGTG ACTCTGCAGT GCAAAGTCTG TGGGCGGCCA
    AAGCCCACCA TCACTTGGAA GGGTCCAGAC CAGAACATCC TTGACACTGA CAACAGCTCA
    GCTACATACA CCGTCTCCTC CTGTGACTCT GGGGAAATCA CCCTGAAAAT CTGCAATCTG
    ATGCCCCAAG ACAGCGGTAT CTATACCTGC ATAGCAGCAA ACGACCATGG TACTGCGTCA
    ACATCTGCTA CTGTTAAAGT GCAAGGTGTT CCAGCGGCCC CTAACCGCCC CATTGCCCAA
    GAGAGAAGCT GCACTTCCGT GATCCTCCGC TGGCTGCCCC CATCCAGCAC GGGAAACTGC
    ACCATTTCCG GTTACACCGT GGAGTACAGA GAGGAAGGTT CCCAGGCCTG GCAGCAGTCA
    GTAGCATCGA CCTTGGACAC CTACCTTGTC ATTGAAGACC TCAGTCCTGG GAGTCCTTAT
    CAGTTCAGAG TCAGTGCCAG TAACCCCTGG GGGATCAGCC TGCCCAGCGA GCCCTCAGAA
    TTCGTGCGAC TTCCAGATTA CGACGCTGCT GCCGATGGTG CCACCATTTC TTGGAAGGAA
    AATTTTGATT CAGCTTATAC TGAGCTGAAT GAAATTGGAA GAGGCCGGTT CTCCATAGTA
    AAGAAGTGCA TTCACAAAGC TACTCGCAAA GATGTCGCTG TGAAATTTGT TAGCAAAAAA
    ATGAAGAAAA AAGAGCAGGC TGCCCACGAG GCTGCCCTGC TTCAGCACCT GCAGCACCCC
    CAATACATCA CTCTCCATGA CACCTATGAG TCCCCCACTT CCTACATCCT CATTTTGGAA
    CTGATGGACG ATGGCCGACT CTTAGACTAT CTTATGAATC ATGATGAGCT GATGGAAGAA
    AAAGTAGCTT TCTACATTCG AGACATCATG GAAGCCCTGC AGTACCTTCA TAACTGCAGA
    GTTGCACATT TGGACATAAA GCCTGAAAAC CTGCTCATTG ACCTACGGAT TCCAGTGCCT
    CGAGTGAAGC TCATTGACTT GGAGGATGCT GTCCAGATCT CAGGTCACTT CCATATCCAC
    CACCTTCTGG GGAACCCCGA GTTTGCTGCC CCAGAAGTGA TCCAGGGCAT CCCTGTATCC
    CTGGGCACCG ACATCTGGAG CATCGGGGTT TTGACATACG TCATGCTGAG TGGGGTCTCC
    CCCTTCTTGG ATGAGAGCAA GGAGGAGACA TGTATCAATG TGTGCAGGGT GGACTTCAGC
    TTCCCCCATG AGTACTTCTG CGGTGTGAGC AATGCTGCCA GAGATTTCAT CAATGTGATC
    CTACAGGAAG ACTTTCGGAG GCGGCCCACA GCAGCCACCT GCCTGCAGCA TCCGTGGCTG
    CAGCCCCACA ATGGCAGCTA CTCCAAGATC CCCCTGGACA CCTCCCGCTT GGCGTGCTTC
    ATCGAGCGCC GCAAGCATCA GAATGATGTG CGCCCCATTC CCAACGTCAA GAGCTACATT
    GTCAATCGGC TAAACCAAGG CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218891
    Clone ID Related Accession (Same CDS sequence) XM_024991357.1
    Accession Version XM_024991357.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    481
    541
    601
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    721
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    841
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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
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    2641
    2701
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    3181
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    4141
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    4441
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    5881
    5941
    6001
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    6301
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    6421
    6481
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    7141
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    7381
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    7681
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    7861
    7921
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    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGACGGACC GCTTCTGGGA ACAGTGGTAT CTTTGGTATC TCCGCTTGCT CCGGCTGCTG 
    GATCGAGGCT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC CATCCTAAAG
    GAAAAAGTGG CCTTCGTGTC TGGGGGCCGT GATAAGCGAG GTGGACCCAT CCTGACCTTC
    CCTGCTCGCA GCAATCATGA CAGAATAAGA CAGGAAGACC TGCGGAAACT GGTGACCTAT
    CTGGCCAGCG TGCCAAGCGA GGACGTGTGC AAACGTGGCT TCACTGTCAT CATCGATATG
    CGGGGGTCCA AGTGGGACCT CATCAAGCCC CTCCTCAAGA CGCTGCAAGA AGCCTTTCCC
    GCTGAGATCC ATGTGGCCCT CATCATCAAA CCCGACAACT TCTGGCAGAA ACAGAAGACC
    AACTTTGGCA GCTCCAAGTT CATCTTTGAG ACAAGCATGG TGTCCGTGGA GGGCCTCACG
    AAGCTGGTGG ACCCCTCCCA GCTGACGGAG GAGTTCGATG GCTCCCTGGA CTACAACCAC
    GAGGAGTGGA TCGAGCTGCG GCTCTCCCTG GAGGAGTTCT TCAACAGCGC CGTACACCTG
    CTCTCACGCC TTGAAGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC CGTGGATGTG
    GAGGGCTCGA GGCGGCTAAT TGATGAACAC ACACAGCTGA AGAAGAAGGT GCTGAAGGCG
    CCTGTGGAGG AGCTGGACCG GGAGGGGCAG CGACTGCTGC AGTGCATCCG CTGCAGTGAC
    GGCTTCTCAG GGCGCAACTG CATCCCGGGC AGTGCCGACT TCCAGAGCTT GGTGCCCAAG
    ATCACCAGCC TGTTGGACAA GCTGCACTCC ACCCGGCAGC ACCTGCACCA GATGTGGCAT
    GTGCGCAAGC TCAAGCTAGA CCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAA
    AAGATGTTTG ACTGGATAAG CCACAACAAG GAGTTATTCC TCCAGAGCCA TACAGAGATT
    GGAGTCAGCT ACCAGCACGC CCTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC
    TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TGGCTTCCCG CCTCTCAGAG
    GCTGGCCACT ATGCTTCACA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG
    AAGAGTTTCG CAGCTGCCCT GGATGAACGC AGCACCATCC TCGCCATGTC TGCTGTGTTC
    CATCAGAAGG CTGAGCAGTT CTTGTCAGGA GTGGATGCCT GGTGCAAGAT GTGCAGTGAA
    GGTGGCCTGC CCTCTGAGAT GCAGGACCTG GAGCTGGCGA TCCACCACCA CCAGTCTTTG
    TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ACGGCAAAGC ACTGCTGGAT
    GTGCTGCAGC GGCCCCTGAG TCCCGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC
    TCCAAGGCGG TGCACCAGGT GCTGGACGTG GTGCACGAAG TGCTGCACCA CCAGCGGCGG
    CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTCCACC AACGCCTGCA GCTCTGCGTC
    TTCCAGCAGG ACGTCCAGCA GGTGTTAGAT TGGATTGAAA ACCACGGCGA GGCCTTTCTC
    AGCAAGCACA CAGGGGTCGG GAAGTCTCTG CACCGAGCTC GGGCCCTGCA GAAGAGGCAC
    GATGACTTTG AAGAGGTGGC TCAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCG
    GCAGAGCAGC TGGCTCAGAC AGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCTAGA
    CACCTGGAGG TGCGCATCCA AGACTTCGTG CGCAGGGTGG AGCAGCGGAA GCTTCTGCTG
    GACATGTCTG TCTCCTTCCA TACTCACACC AAAGAGCTAT GGACATGGAT GGAAGACCTT
    CAGAAGGAGA TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAACTGATC
    AAGCAGTTCC AGCAGCAGCA GACCGCCACT CTGGATGCCA CACTTAATGT CATCAAGGAG
    GGCGAAGACC TTATCCAGCA ACTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCAGCGAG
    GCCAGGGACT CAGCTGTGTC CAACAACAAG ACGCCCCATA GTAGCTCCAT CAGCCACATA
    GAGTCGGTGC TGCAGCAGCT GGATGACGCC CAGGTGCAAA TGGAGGAGCT GTTCCACGAA
    CGCAAGATCA AGCTGGACAT CTTCCTGCAG CTGCGCATCT TTGAGCAGTA CACCATTGAG
    GTGACAGCAG AGCTCGACGC TTGGAATGAG GATTTGCTTC GGCAGATGAA TGACTTCAAC
    ACGGAGGATC TAACACTGGC GGAACAGCGG CTGCAGCGCC ACACAGAGCG GAAGCTAGCC
    ATGAACAACA TGACCTTTGA GGTCATCCAG CAAGGCCAGG ATCTGCACCA GTACATCATG
    GAGGTCCAGG CCTCAGGGAT TGAGTTAATC TGTGAAAAAG ACATTGATCT GGCAGCCCAG
    GTACAAGAGC TATTAGAGTT TCTCCATGAG AAACAGCATG AGTTGGAGCT CAACGCAGAG
    CAGACCCACA AGCGACTTGA GCAGTGCCTC CAACTGCGGC ACCTCCAGGC CGAGGTCAAA
    CAGGTCCTAG GATGGATCCG CAATGGGGAG TCAATGCTCA ATGCCAGCCT GGTCAACGCC
    AGCTCTTTGT CTGAAGCAGA GCAGCTACAG CGGGAGCACG AGCAGTTCCA ACTAGCCATC
    GAGAAGACAC ACCAGAGCGC CCTGCAGGTT CAGCAGAAAG CTGAGGCGCT GCTCCAGGCT
    GGTCACTATG ATGCAGATGC CATCCGGGAA TGTGCTGAGA AGGTGGCCCT CCACTGGCAG
    CAACTCATGC TCAAGATGGA AGACCGACTG AAACTGGTCA ATGCCTCAGT GGCCTTTTAT
    AAAACTTCTG AGCAGGTCTG TAGTGTCCTG GAGAGCTTAG AGCAAGAATA CCGAAGAGAT
    GAGGACTGGT GTGGTGGACG AGATAAATTG GGGCCAGCAG CAGAGATCGA CCACGTCATT
    CCACTCATCA GTAAACATTT GGAACAAAAG GAAGCCTTTC TTAAGGCCTG CACCCTGGCA
    CGGCGGAATG CTGAGGTCTT TCTCAAGTAC ATCCATAGGA ACAACGTCAG CATGCCCAGT
    GCTGCCAGCC ACACCCGAGG ACCCGAACAG CAAGTGAAAG CCATCCTGAG TGAGCTCTTA
    CAGAGGGAGA ACCGTGTCCT GCACTTCTGG ACATTGAAGA AGCGGCGGTT AGACCAGTGT
    CAGCAATACG TGGTATTTGA GCGAAGCGCC AAGCAGGCAC TAGACTGGAT CCAAGAAACA
    GGTGAATATT ACCTCTCAAC ACATACCTCC ACCGGGGAGA CCACAGAGGA GACTCAGGAA
    CTGCTGAAAG AATATGGGGA GTTCAGGGTG CCTGCCAAGC AAACAAAGGA GAAGGTGAAG
    CTTCTGATTC AGCTGGCTGA TAGCTTTGTG GAAAAAGGTC ACATTCATGC CACAGAGATC
    AGGAAATGGG TGACCACAGT GGACAAGCAT TACAGAGACT TCTCACTGAG GATGGGAAAA
    TACCGGTACT CCCTGGAGAA GGCCCTAGGA GTCAACACGG AGGATAACAA GGACCTGGAG
    CTGGACATCA TCCCAGCAAG CCTTTCAGAT CGTGAGGTCA AGCTCCGGGA TGCCAACCAC
    GAGGTCAATG AAGAGAAGCG GAAATCAGCC AGGAAAAAAG AATTCATTAT GGCAGAATTG
    CTCCAGACAG AGAAGGCTTA TGTGAGGGAC TTACATGAGT GCTTAGAGAC CTACCTGTGG
    GAAATGACCA GTGGTGTGGA GGAGATCCCC CCTGGGATCC TTAATAAGGA GCATATCATC
    TTTGGCAACA TCCAAGAGAT CTACGATTTT CATAACAACA TCTTCCTCAA AGAACTGGAG
    AAGTATGAGC AGCTGCCTGA GGATGTGGGA CACTGCTTTG TTACTTGGGC AGACAAATTT
    CAGATGTACG TCACCTACTG TAAAAACAAG CCTGATTCCA ACCAGCTGAT CCTGGAGCAT
    GCAGGCACCT TCTTTGATGA GATACAGCAG CGGCATGGTC TGGCCAACTC CATTTCTTCC
    TACCTAATTA AGCCTGTCCA GAGGATTACC AAGTACCAGC TGCTCCTGAA GGAACTTTTA
    ACTTGCTGTG AAGAAGGAAA AGGGGAGCTC AAGGAAGGCC TGGAGGTGAT GCTTAGTGTC
    CCAAAGAGAG CCAACGATGC CATGCACGTC AGCATGCTGG AAGGGTTCGA CGAGAACCTG
    GACGTGCAGG GGGAGCTGAT TTTACAAGAT GCCTTTCAAG TGTGGGACCC CAAGTCGCTG
    ATCCGGAAAG GGCGGGAGCG ACACCTATTC CTCTTTGAGA TCTCCTTGGT TTTCAGCAAG
    GAGATCAAAG ACTCTTCAGG ACACACAAAA TATGTTTACA AGAACAAGCT ACTGACCTCA
    GAGCTGGGTG TGACCGAACA CGTAGAGGGC GATCCCTGCA AATTCGCCTT GTGGTCTGGG
    CGCACCCCAT CCTCAGACAA TAAAACAGTG CTGAAAGCCT CCAACATAGA AACCAAGCAG
    GAGTGGATCA AGAACATTCG AGAGGTGATT CAAGAAAGGA TCATTCACCT GAAAGGAGCT
    TTGAAGGAGC CAATTCAGCT CCCCAAAACA CCAGCCAAAC AGAGGAACAA CAGTAAGAGG
    GATGGAGTGG AGGATGTTGA CAGCCAGGGG GATGGGAGCA GCCAGCCAGA CACCATCTCC
    ATTGCCTCTA GGACCTCCCA GAACACAGCG GACAGTGACA AGCTCTCTGG CGGCTGTGAG
    TTGACCGTGG TGCTCCAGGA CTTCAACGCA GGCCACAGCA GCGAGCTGAG CATCCAGGTG
    GGACAGACGG TGGAGCTGCT AGAGCGGCCG AGCGAGCGGC CGGGCTGGTG TCTGGTCCGA
    ACCACAGAGC GGAGCCCCCC CCAGGAGGGC CTGGTGCCCA GCAGCGCCCT GTGCATCTCC
    CACTCCCGAA GCAGCGTGGA GATGGACTGC TTCTTCCCCC TGGTGAAAGA TGCGTACTCT
    CATTCCTCAA GTGAAAACGG AGGCAAGTCC GAGTCGGTGG CCAACCTGCA GGCCCAACCA
    TCCCTGAACT CCATCCACAG CTCCCCTGGG CCCAAGCGTT CCACCAACAC CCTGAAGAAG
    TGGCTGACGA GTCCCGTGCG CCGGCTTAAC AGTGGGAAAG CAGATGGAAA CATCAAGAAG
    CAGAAGAAAG TACGAGATGG TCGGAAGAGC TTTGACCTGG GATCTCCCAA GCCTGGGGAT
    GAGACGACCC CTCAGGGAGA CAGCGCTGAT GAGAAGAGCA AGAAAGGTTG GGGTGAAGAT
    GAGGCAGATG AAGAGTCACA CACACCCCTC CCCCCGCCTA TGAAGATCTT TGACAACGAC
    CCCACGCAGG ACGAAATGTC CTCCTCTTTG CTAGCAGCCC GGCAGGCTTC CACCGAAGTA
    CCTACTGCTG CAGACCTTGT CAGTGCGATA GAACAGTTGG TCAAAAGCAA GCTGAGTCTG
    GAAGGAGGCT CTTACCGAGG GAGCTTGAAA GACCCCACAG GCTGCCTGAA TGAAGGCATG
    ACCCCACCCA CACCTCCCAG AAATCTGGAA GAAGAACAGA AAGCCAAGGC CCTGAGAGGC
    AGGATGTTTG TCCTGAATGA ACTGGTACAA ACAGAGAAGG ACTATGTCAA GGACCTGGGA
    ATTGTGGTGG AGGGTTTCAT GAAGAGAATA GAAGAAAAGG GTGTCCCCGA GGACATGCGA
    GGAAAGGATA AAATCGTGTT TGGAAATATT CACCAGATTT ATGATTGGCA TAAGGATGTT
    TTCCTGGGTG AACTGGAAAA ATGTATCCAG GAGCAAGACA GATTGGCACA GCTCTTTATT
    AAATATGAGC GGAAGCTGCA CATCTATGTG TGGTACTGCC AGAACAAGCC GCGCTCCGAG
    TACATCGTCG CCGAGTACGA CGCCTACTTT GAAGAGGTGA AACAAGAGAT AAATCAAAGG
    CTGACGCTTA GCGACTTCCT TATCAAGCCC ATTCAGAGAA TAACAAAATA TCAATTGCTC
    CTCAAGGACT TCCTGAGATA CAGCGAGAAG GCTGGTTTGG AGTGTTCAGA TATTGAGAAA
    GCAGTGGAGT TAATGTGCCT TGTTCCAAAA CGCTGCAATG ACATGATGAA CCTGGGGCGC
    CTGCAGGGCT TTGAGGGGAC CCTGACTGCT CAGGGCAAGC TGCTGCAGCA GGACACATTC
    TACGTGATTG AGCTGGACGC TGGCATGCAG TCGCGGACCA AGGAGAGGCG TGTGTTTCTC
    TTTGAGCAAA TCGTCATCTT CAGTGAACTG CTCAGGAAGG GATCCCTCAC CCCAGGCTAC
    ATGTTCAAAA GGAGCATCAA GATGAACTAC TTGGTCCTGG AGGAGAACGT GGACAATGAC
    CCCTGCAAGT TTGCGCTTCT GAACAGGGAG ACTTCAGAGA GGGTTATTCT GCAAGCCGCC
    AATGCTGACA TCCAGCAGGC CTGGGTGCAG GACATCAACC AAGTCTTAGA AACACAGCGA
    GACTTCTTAA ATGCCCTACA GTCGCCCATT GAGTATCAGC GGAAAGAAAG GAGCACAGCC
    GTGATGAGGT CCCAGCCTGC TAGGGTTCCC CAAGCCAGCC CCAGGCCCTA CTCCTCCATC
    CCCGTGGGCT CCGAGAAGCC CCCAAAGGGC TCCAGCTATA ACCCGCCTCT GCCACCCCTG
    AAGATATCTA CCTCCAATGG CAGTCCAGGG TTTGACTACC ACCAGGCTGG GGACAAGTTT
    GAGGCCAGCA AGAATGACCT GGGAGGCTGC AACGGGGCCT CATCCATGGC CGTGATCAAA
    GATTATTATG CACTGAAGGA GAACGAAATC TGTGTGAGCC AAGGTGAGGT GGTCCAGGTC
    CTCGCCGTCA ACCAGCAGAA CATGTGCCTG GTGTACCAGC CCGCTAGCGA CCACTCCCCC
    GCCGCTGAGG GCTGGGTCCC TGGCAGCATC CTGACGCCCC TCACCAAAGC CATGGCAGCC
    ACAGAAAATA GTGACGGGAG CATCAAGAAG TCATGTTCAT GGCATACTCT ACGCATGAGA
    AAGCGGGCAG AAGTGGAGAA CACGGGTAAA AATGAAGCCA CAGGGCCTCG TAAACCCAAG
    GATATTCTGG GCAACAAAGT CTCTGTTAAA GAGACGAACA GTTCCGAGGA GTCAGAGTGT
    GATGATCTTG ACCCTAATAC TAGCATGGAG ATCTTAAATC CAAATTTCAT CCAAGAAGTG
    GCGCCAGAAT TCCTCGTGCC CTTAGTGGAT GTGACCTGCT TGCTTGGGGA CACAGTGACT
    CTGCAGTGCA AAGTCTGTGG GCGGCCAAAG CCCACCATCA CTTGGAAGGG TCCAGACCAG
    AACATCCTTG ACACTGACAA CAGCTCAGCT ACATACACCG TCTCCTCCTG TGACTCTGGG
    GAAATCACCC TGAAAATCTG CAATCTGATG CCCCAAGACA GCGGTATCTA TACCTGCATA
    GCAGCAAACG ACCATGGTAC TGCGTCAACA TCTGCTACTG TTAAAGTGCA AGGTGTTCCA
    GCGGCCCCTA ACCGCCCCAT TGCCCAAGAG AGAAGCTGCA CTTCCGTGAT CCTCCGCTGG
    CTGCCCCCAT CCAGCACGGG AAACTGCACC ATTTCCGGTT ACACCGTGGA GTACAGAGAG
    GAAGGTTCCC AGGCCTGGCA GCAGTCAGTA GCATCGACCT TGGACACCTA CCTTGTCATT
    GAAGACCTCA GTCCTGGGAG TCCTTATCAG TTCAGAGTCA GTGCCAGTAA CCCCTGGGGG
    ATCAGCCTGC CCAGCGAGCC CTCAGAATTC GTGCGACTTC CAGATTACGA CGCTGCTGCC
    GATGGTGCCA CCATTTCTTG GAAGGAAAAT TTTGATTCAG CTTATACTGA GCTGAATGAA
    ATTGGAAGAG GCCGGTTCTC CATAGTAAAG AAGTGCATTC ACAAAGCTAC TCGCAAAGAT
    GTCGCTGTGA AATTTGTTAG CAAAAAAATG AAGAAAAAAG AGCAGGCTGC CCACGAGGCT
    GCCCTGCTTC AGCACCTGCA GCACCCCCAA TACATCACTC TCCATGACAC CTATGAGTCC
    CCCACTTCCT ACATCCTCAT TTTGGAACTG ATGGACGATG GCCGACTCTT AGACTATCTT
    ATGAATCATG ATGAGCTGAT GGAAGAAAAA GTAGCTTTCT ACATTCGAGA CATCATGGAA
    GCCCTGCAGT ACCTTCATAA CTGCAGAGTT GCACATTTGG ACATAAAGCC TGAAAACCTG
    CTCATTGACC TACGGATTCC AGTGCCTCGA GTGAAGCTCA TTGACTTGGA GGATGCTGTC
    CAGATCTCAG GTCACTTCCA TATCCACCAC CTTCTGGGGA ACCCCGAGTT TGCTGCCCCA
    GAAGTGATCC AGGGCATCCC TGTATCCCTG GGCACCGACA TCTGGAGCAT CGGGGTTTTG
    ACATACGTCA TGCTGAGTGG GGTCTCCCCC TTCTTGGATG AGAGCAAGGA GGAGACATGT
    ATCAATGTGT GCAGGGTGGA CTTCAGCTTC CCCCATGAGT ACTTCTGCGG TGTGAGCAAT
    GCTGCCAGAG ATTTCATCAA TGTGATCCTA CAGGAAGACT TTCGGAGGCG GCCCACAGCA
    GCCACCTGCC TGCAGCATCC GTGGCTGCAG CCCCACAATG GCAGCTACTC CAAGATCCCC
    CTGGACACCT CCCGCTTGGC GTGCTTCATC GAGCGCCGCA AGCATCAGAA TGATGTGCGC
    CCCATTCCCA ACGTCAAGAG CTACATTGTC AATCGGCTAA ACCAAGGCAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847125.1
    CDS1..8934
    Translation

    Target ORF information:

    RefSeq Version XM_024991357.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991357.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGACGGACC GCTTCTGGGA ACAGTGGTAT CTTTGGTATC TCCGCTTGCT CCGGCTGCTG 
    GATCGAGGCT CTTTTCGGAA TGATGGTTTG AAAGCTTCTG ATGTCCTTCC CATCCTAAAG
    GAAAAAGTGG CCTTCGTGTC TGGGGGCCGT GATAAGCGAG GTGGACCCAT CCTGACCTTC
    CCTGCTCGCA GCAATCATGA CAGAATAAGA CAGGAAGACC TGCGGAAACT GGTGACCTAT
    CTGGCCAGCG TGCCAAGCGA GGACGTGTGC AAACGTGGCT TCACTGTCAT CATCGATATG
    CGGGGGTCCA AGTGGGACCT CATCAAGCCC CTCCTCAAGA CGCTGCAAGA AGCCTTTCCC
    GCTGAGATCC ATGTGGCCCT CATCATCAAA CCCGACAACT TCTGGCAGAA ACAGAAGACC
    AACTTTGGCA GCTCCAAGTT CATCTTTGAG ACAAGCATGG TGTCCGTGGA GGGCCTCACG
    AAGCTGGTGG ACCCCTCCCA GCTGACGGAG GAGTTCGATG GCTCCCTGGA CTACAACCAC
    GAGGAGTGGA TCGAGCTGCG GCTCTCCCTG GAGGAGTTCT TCAACAGCGC CGTACACCTG
    CTCTCACGCC TTGAAGACCT GCAGGAGATG CTGGCCCGGA AGGAGTTCCC CGTGGATGTG
    GAGGGCTCGA GGCGGCTAAT TGATGAACAC ACACAGCTGA AGAAGAAGGT GCTGAAGGCG
    CCTGTGGAGG AGCTGGACCG GGAGGGGCAG CGACTGCTGC AGTGCATCCG CTGCAGTGAC
    GGCTTCTCAG GGCGCAACTG CATCCCGGGC AGTGCCGACT TCCAGAGCTT GGTGCCCAAG
    ATCACCAGCC TGTTGGACAA GCTGCACTCC ACCCGGCAGC ACCTGCACCA GATGTGGCAT
    GTGCGCAAGC TCAAGCTAGA CCAGTGCTTC CAGCTGCGGC TCTTCGAGCA GGATGCTGAA
    AAGATGTTTG ACTGGATAAG CCACAACAAG GAGTTATTCC TCCAGAGCCA TACAGAGATT
    GGAGTCAGCT ACCAGCACGC CCTAGACCTC CAGACACAGC ACAATCACTT TGCCATGAAC
    TCCATGAATG CCTATGTCAA CATCAACCGC ATCATGTCTG TGGCTTCCCG CCTCTCAGAG
    GCTGGCCACT ATGCTTCACA GCAAATCAAG CAGATCTCCA CGCAGCTAGA CCAGGAGTGG
    AAGAGTTTCG CAGCTGCCCT GGATGAACGC AGCACCATCC TCGCCATGTC TGCTGTGTTC
    CATCAGAAGG CTGAGCAGTT CTTGTCAGGA GTGGATGCCT GGTGCAAGAT GTGCAGTGAA
    GGTGGCCTGC CCTCTGAGAT GCAGGACCTG GAGCTGGCGA TCCACCACCA CCAGTCTTTG
    TATGAGCAGG TGACCCAGGC CTACACAGAG GTCAGCCAGG ACGGCAAAGC ACTGCTGGAT
    GTGCTGCAGC GGCCCCTGAG TCCCGGGAAC TCTGAGTCCC TCACAGCCAC AGCCAACTAC
    TCCAAGGCGG TGCACCAGGT GCTGGACGTG GTGCACGAAG TGCTGCACCA CCAGCGGCGG
    CTCGAGAGCA TCTGGCAGCA CCGCAAGGTG CGGCTCCACC AACGCCTGCA GCTCTGCGTC
    TTCCAGCAGG ACGTCCAGCA GGTGTTAGAT TGGATTGAAA ACCACGGCGA GGCCTTTCTC
    AGCAAGCACA CAGGGGTCGG GAAGTCTCTG CACCGAGCTC GGGCCCTGCA GAAGAGGCAC
    GATGACTTTG AAGAGGTGGC TCAGAATACA TACACCAATG CGGACAAGCT CCTAGAAGCG
    GCAGAGCAGC TGGCTCAGAC AGGGGAATGT GACCCGGAGG AGATCTACAA GGCAGCTAGA
    CACCTGGAGG TGCGCATCCA AGACTTCGTG CGCAGGGTGG AGCAGCGGAA GCTTCTGCTG
    GACATGTCTG TCTCCTTCCA TACTCACACC AAAGAGCTAT GGACATGGAT GGAAGACCTT
    CAGAAGGAGA TGCTGGAGGA TGTCTGTGCA GACTCTGTGG ATGCGGTCCA GGAACTGATC
    AAGCAGTTCC AGCAGCAGCA GACCGCCACT CTGGATGCCA CACTTAATGT CATCAAGGAG
    GGCGAAGACC TTATCCAGCA ACTCAGGTCA GCGCCTCCCT CCCTGGGGGA GCCCAGCGAG
    GCCAGGGACT CAGCTGTGTC CAACAACAAG ACGCCCCATA GTAGCTCCAT CAGCCACATA
    GAGTCGGTGC TGCAGCAGCT GGATGACGCC CAGGTGCAAA TGGAGGAGCT GTTCCACGAA
    CGCAAGATCA AGCTGGACAT CTTCCTGCAG CTGCGCATCT TTGAGCAGTA CACCATTGAG
    GTGACAGCAG AGCTCGACGC TTGGAATGAG GATTTGCTTC GGCAGATGAA TGACTTCAAC
    ACGGAGGATC TAACACTGGC GGAACAGCGG CTGCAGCGCC ACACAGAGCG GAAGCTAGCC
    ATGAACAACA TGACCTTTGA GGTCATCCAG CAAGGCCAGG ATCTGCACCA GTACATCATG
    GAGGTCCAGG CCTCAGGGAT TGAGTTAATC TGTGAAAAAG ACATTGATCT GGCAGCCCAG
    GTACAAGAGC TATTAGAGTT TCTCCATGAG AAACAGCATG AGTTGGAGCT CAACGCAGAG
    CAGACCCACA AGCGACTTGA GCAGTGCCTC CAACTGCGGC ACCTCCAGGC CGAGGTCAAA
    CAGGTCCTAG GATGGATCCG CAATGGGGAG TCAATGCTCA ATGCCAGCCT GGTCAACGCC
    AGCTCTTTGT CTGAAGCAGA GCAGCTACAG CGGGAGCACG AGCAGTTCCA ACTAGCCATC
    GAGAAGACAC ACCAGAGCGC CCTGCAGGTT CAGCAGAAAG CTGAGGCGCT GCTCCAGGCT
    GGTCACTATG ATGCAGATGC CATCCGGGAA TGTGCTGAGA AGGTGGCCCT CCACTGGCAG
    CAACTCATGC TCAAGATGGA AGACCGACTG AAACTGGTCA ATGCCTCAGT GGCCTTTTAT
    AAAACTTCTG AGCAGGTCTG TAGTGTCCTG GAGAGCTTAG AGCAAGAATA CCGAAGAGAT
    GAGGACTGGT GTGGTGGACG AGATAAATTG GGGCCAGCAG CAGAGATCGA CCACGTCATT
    CCACTCATCA GTAAACATTT GGAACAAAAG GAAGCCTTTC TTAAGGCCTG CACCCTGGCA
    CGGCGGAATG CTGAGGTCTT TCTCAAGTAC ATCCATAGGA ACAACGTCAG CATGCCCAGT
    GCTGCCAGCC ACACCCGAGG ACCCGAACAG CAAGTGAAAG CCATCCTGAG TGAGCTCTTA
    CAGAGGGAGA ACCGTGTCCT GCACTTCTGG ACATTGAAGA AGCGGCGGTT AGACCAGTGT
    CAGCAATACG TGGTATTTGA GCGAAGCGCC AAGCAGGCAC TAGACTGGAT CCAAGAAACA
    GGTGAATATT ACCTCTCAAC ACATACCTCC ACCGGGGAGA CCACAGAGGA GACTCAGGAA
    CTGCTGAAAG AATATGGGGA GTTCAGGGTG CCTGCCAAGC AAACAAAGGA GAAGGTGAAG
    CTTCTGATTC AGCTGGCTGA TAGCTTTGTG GAAAAAGGTC ACATTCATGC CACAGAGATC
    AGGAAATGGG TGACCACAGT GGACAAGCAT TACAGAGACT TCTCACTGAG GATGGGAAAA
    TACCGGTACT CCCTGGAGAA GGCCCTAGGA GTCAACACGG AGGATAACAA GGACCTGGAG
    CTGGACATCA TCCCAGCAAG CCTTTCAGAT CGTGAGGTCA AGCTCCGGGA TGCCAACCAC
    GAGGTCAATG AAGAGAAGCG GAAATCAGCC AGGAAAAAAG AATTCATTAT GGCAGAATTG
    CTCCAGACAG AGAAGGCTTA TGTGAGGGAC TTACATGAGT GCTTAGAGAC CTACCTGTGG
    GAAATGACCA GTGGTGTGGA GGAGATCCCC CCTGGGATCC TTAATAAGGA GCATATCATC
    TTTGGCAACA TCCAAGAGAT CTACGATTTT CATAACAACA TCTTCCTCAA AGAACTGGAG
    AAGTATGAGC AGCTGCCTGA GGATGTGGGA CACTGCTTTG TTACTTGGGC AGACAAATTT
    CAGATGTACG TCACCTACTG TAAAAACAAG CCTGATTCCA ACCAGCTGAT CCTGGAGCAT
    GCAGGCACCT TCTTTGATGA GATACAGCAG CGGCATGGTC TGGCCAACTC CATTTCTTCC
    TACCTAATTA AGCCTGTCCA GAGGATTACC AAGTACCAGC TGCTCCTGAA GGAACTTTTA
    ACTTGCTGTG AAGAAGGAAA AGGGGAGCTC AAGGAAGGCC TGGAGGTGAT GCTTAGTGTC
    CCAAAGAGAG CCAACGATGC CATGCACGTC AGCATGCTGG AAGGGTTCGA CGAGAACCTG
    GACGTGCAGG GGGAGCTGAT TTTACAAGAT GCCTTTCAAG TGTGGGACCC CAAGTCGCTG
    ATCCGGAAAG GGCGGGAGCG ACACCTATTC CTCTTTGAGA TCTCCTTGGT TTTCAGCAAG
    GAGATCAAAG ACTCTTCAGG ACACACAAAA TATGTTTACA AGAACAAGCT ACTGACCTCA
    GAGCTGGGTG TGACCGAACA CGTAGAGGGC GATCCCTGCA AATTCGCCTT GTGGTCTGGG
    CGCACCCCAT CCTCAGACAA TAAAACAGTG CTGAAAGCCT CCAACATAGA AACCAAGCAG
    GAGTGGATCA AGAACATTCG AGAGGTGATT CAAGAAAGGA TCATTCACCT GAAAGGAGCT
    TTGAAGGAGC CAATTCAGCT CCCCAAAACA CCAGCCAAAC AGAGGAACAA CAGTAAGAGG
    GATGGAGTGG AGGATGTTGA CAGCCAGGGG GATGGGAGCA GCCAGCCAGA CACCATCTCC
    ATTGCCTCTA GGACCTCCCA GAACACAGCG GACAGTGACA AGCTCTCTGG CGGCTGTGAG
    TTGACCGTGG TGCTCCAGGA CTTCAACGCA GGCCACAGCA GCGAGCTGAG CATCCAGGTG
    GGACAGACGG TGGAGCTGCT AGAGCGGCCG AGCGAGCGGC CGGGCTGGTG TCTGGTCCGA
    ACCACAGAGC GGAGCCCCCC CCAGGAGGGC CTGGTGCCCA GCAGCGCCCT GTGCATCTCC
    CACTCCCGAA GCAGCGTGGA GATGGACTGC TTCTTCCCCC TGGTGAAAGA TGCGTACTCT
    CATTCCTCAA GTGAAAACGG AGGCAAGTCC GAGTCGGTGG CCAACCTGCA GGCCCAACCA
    TCCCTGAACT CCATCCACAG CTCCCCTGGG CCCAAGCGTT CCACCAACAC CCTGAAGAAG
    TGGCTGACGA GTCCCGTGCG CCGGCTTAAC AGTGGGAAAG CAGATGGAAA CATCAAGAAG
    CAGAAGAAAG TACGAGATGG TCGGAAGAGC TTTGACCTGG GATCTCCCAA GCCTGGGGAT
    GAGACGACCC CTCAGGGAGA CAGCGCTGAT GAGAAGAGCA AGAAAGGTTG GGGTGAAGAT
    GAGGCAGATG AAGAGTCACA CACACCCCTC CCCCCGCCTA TGAAGATCTT TGACAACGAC
    CCCACGCAGG ACGAAATGTC CTCCTCTTTG CTAGCAGCCC GGCAGGCTTC CACCGAAGTA
    CCTACTGCTG CAGACCTTGT CAGTGCGATA GAACAGTTGG TCAAAAGCAA GCTGAGTCTG
    GAAGGAGGCT CTTACCGAGG GAGCTTGAAA GACCCCACAG GCTGCCTGAA TGAAGGCATG
    ACCCCACCCA CACCTCCCAG AAATCTGGAA GAAGAACAGA AAGCCAAGGC CCTGAGAGGC
    AGGATGTTTG TCCTGAATGA ACTGGTACAA ACAGAGAAGG ACTATGTCAA GGACCTGGGA
    ATTGTGGTGG AGGGTTTCAT GAAGAGAATA GAAGAAAAGG GTGTCCCCGA GGACATGCGA
    GGAAAGGATA AAATCGTGTT TGGAAATATT CACCAGATTT ATGATTGGCA TAAGGATGTT
    TTCCTGGGTG AACTGGAAAA ATGTATCCAG GAGCAAGACA GATTGGCACA GCTCTTTATT
    AAATATGAGC GGAAGCTGCA CATCTATGTG TGGTACTGCC AGAACAAGCC GCGCTCCGAG
    TACATCGTCG CCGAGTACGA CGCCTACTTT GAAGAGGTGA AACAAGAGAT AAATCAAAGG
    CTGACGCTTA GCGACTTCCT TATCAAGCCC ATTCAGAGAA TAACAAAATA TCAATTGCTC
    CTCAAGGACT TCCTGAGATA CAGCGAGAAG GCTGGTTTGG AGTGTTCAGA TATTGAGAAA
    GCAGTGGAGT TAATGTGCCT TGTTCCAAAA CGCTGCAATG ACATGATGAA CCTGGGGCGC
    CTGCAGGGCT TTGAGGGGAC CCTGACTGCT CAGGGCAAGC TGCTGCAGCA GGACACATTC
    TACGTGATTG AGCTGGACGC TGGCATGCAG TCGCGGACCA AGGAGAGGCG TGTGTTTCTC
    TTTGAGCAAA TCGTCATCTT CAGTGAACTG CTCAGGAAGG GATCCCTCAC CCCAGGCTAC
    ATGTTCAAAA GGAGCATCAA GATGAACTAC TTGGTCCTGG AGGAGAACGT GGACAATGAC
    CCCTGCAAGT TTGCGCTTCT GAACAGGGAG ACTTCAGAGA GGGTTATTCT GCAAGCCGCC
    AATGCTGACA TCCAGCAGGC CTGGGTGCAG GACATCAACC AAGTCTTAGA AACACAGCGA
    GACTTCTTAA ATGCCCTACA GTCGCCCATT GAGTATCAGC GGAAAGAAAG GAGCACAGCC
    GTGATGAGGT CCCAGCCTGC TAGGGTTCCC CAAGCCAGCC CCAGGCCCTA CTCCTCCATC
    CCCGTGGGCT CCGAGAAGCC CCCAAAGGGC TCCAGCTATA ACCCGCCTCT GCCACCCCTG
    AAGATATCTA CCTCCAATGG CAGTCCAGGG TTTGACTACC ACCAGGCTGG GGACAAGTTT
    GAGGCCAGCA AGAATGACCT GGGAGGCTGC AACGGGGCCT CATCCATGGC CGTGATCAAA
    GATTATTATG CACTGAAGGA GAACGAAATC TGTGTGAGCC AAGGTGAGGT GGTCCAGGTC
    CTCGCCGTCA ACCAGCAGAA CATGTGCCTG GTGTACCAGC CCGCTAGCGA CCACTCCCCC
    GCCGCTGAGG GCTGGGTCCC TGGCAGCATC CTGACGCCCC TCACCAAAGC CATGGCAGCC
    ACAGAAAATA GTGACGGGAG CATCAAGAAG TCATGTTCAT GGCATACTCT ACGCATGAGA
    AAGCGGGCAG AAGTGGAGAA CACGGGTAAA AATGAAGCCA CAGGGCCTCG TAAACCCAAG
    GATATTCTGG GCAACAAAGT CTCTGTTAAA GAGACGAACA GTTCCGAGGA GTCAGAGTGT
    GATGATCTTG ACCCTAATAC TAGCATGGAG ATCTTAAATC CAAATTTCAT CCAAGAAGTG
    GCGCCAGAAT TCCTCGTGCC CTTAGTGGAT GTGACCTGCT TGCTTGGGGA CACAGTGACT
    CTGCAGTGCA AAGTCTGTGG GCGGCCAAAG CCCACCATCA CTTGGAAGGG TCCAGACCAG
    AACATCCTTG ACACTGACAA CAGCTCAGCT ACATACACCG TCTCCTCCTG TGACTCTGGG
    GAAATCACCC TGAAAATCTG CAATCTGATG CCCCAAGACA GCGGTATCTA TACCTGCATA
    GCAGCAAACG ACCATGGTAC TGCGTCAACA TCTGCTACTG TTAAAGTGCA AGGTGTTCCA
    GCGGCCCCTA ACCGCCCCAT TGCCCAAGAG AGAAGCTGCA CTTCCGTGAT CCTCCGCTGG
    CTGCCCCCAT CCAGCACGGG AAACTGCACC ATTTCCGGTT ACACCGTGGA GTACAGAGAG
    GAAGGTTCCC AGGCCTGGCA GCAGTCAGTA GCATCGACCT TGGACACCTA CCTTGTCATT
    GAAGACCTCA GTCCTGGGAG TCCTTATCAG TTCAGAGTCA GTGCCAGTAA CCCCTGGGGG
    ATCAGCCTGC CCAGCGAGCC CTCAGAATTC GTGCGACTTC CAGATTACGA CGCTGCTGCC
    GATGGTGCCA CCATTTCTTG GAAGGAAAAT TTTGATTCAG CTTATACTGA GCTGAATGAA
    ATTGGAAGAG GCCGGTTCTC CATAGTAAAG AAGTGCATTC ACAAAGCTAC TCGCAAAGAT
    GTCGCTGTGA AATTTGTTAG CAAAAAAATG AAGAAAAAAG AGCAGGCTGC CCACGAGGCT
    GCCCTGCTTC AGCACCTGCA GCACCCCCAA TACATCACTC TCCATGACAC CTATGAGTCC
    CCCACTTCCT ACATCCTCAT TTTGGAACTG ATGGACGATG GCCGACTCTT AGACTATCTT
    ATGAATCATG ATGAGCTGAT GGAAGAAAAA GTAGCTTTCT ACATTCGAGA CATCATGGAA
    GCCCTGCAGT ACCTTCATAA CTGCAGAGTT GCACATTTGG ACATAAAGCC TGAAAACCTG
    CTCATTGACC TACGGATTCC AGTGCCTCGA GTGAAGCTCA TTGACTTGGA GGATGCTGTC
    CAGATCTCAG GTCACTTCCA TATCCACCAC CTTCTGGGGA ACCCCGAGTT TGCTGCCCCA
    GAAGTGATCC AGGGCATCCC TGTATCCCTG GGCACCGACA TCTGGAGCAT CGGGGTTTTG
    ACATACGTCA TGCTGAGTGG GGTCTCCCCC TTCTTGGATG AGAGCAAGGA GGAGACATGT
    ATCAATGTGT GCAGGGTGGA CTTCAGCTTC CCCCATGAGT ACTTCTGCGG TGTGAGCAAT
    GCTGCCAGAG ATTTCATCAA TGTGATCCTA CAGGAAGACT TTCGGAGGCG GCCCACAGCA
    GCCACCTGCC TGCAGCATCC GTGGCTGCAG CCCCACAATG GCAGCTACTC CAAGATCCCC
    CTGGACACCT CCCGCTTGGC GTGCTTCATC GAGCGCCGCA AGCATCAGAA TGATGTGCGC
    CCCATTCCCA ACGTCAAGAG CTACATTGTC AATCGGCTAA ACCAAGGCAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218892
    Clone ID Related Accession (Same CDS sequence) XM_024991361.1
    Accession Version XM_024991361.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8871bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CACACCAGAG CGCCCTGCAG
    GTTCAGCAGA AAGCTGAGGC GCTGCTCCAG GCTGGTCACT ATGATGCAGA TGCCATCCGG
    GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAGCAACTCA TGCTCAAGAT GGAAGACCGA
    CTGAAACTGG TCAATGCCTC AGTGGCCTTT TATAAAACTT CTGAGCAGGT CTGTAGTGTC
    CTGGAGAGCT TAGAGCAAGA ATACCGAAGA GATGAGGACT GGTGTGGTGG ACGAGATAAA
    TTGGGGCCAG CAGCAGAGAT CGACCACGTC ATTCCACTCA TCAGTAAACA TTTGGAACAA
    AAGGAAGCCT TTCTTAAGGC CTGCACCCTG GCACGGCGGA ATGCTGAGGT CTTTCTCAAG
    TACATCCATA GGAACAACGT CAGCATGCCC AGTGCTGCCA GCCACACCCG AGGACCCGAA
    CAGCAAGTGA AAGCCATCCT GAGTGAGCTC TTACAGAGGG AGAACCGTGT CCTGCACTTC
    TGGACATTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ACGTGGTATT TGAGCGAAGC
    GCCAAGCAGG CACTAGACTG GATCCAAGAA ACAGGTGAAT ATTACCTCTC AACACATACC
    TCCACCGGGG AGACCACAGA GGAGACTCAG GAACTGCTGA AAGAATATGG GGAGTTCAGG
    GTGCCTGCCA AGCAAACAAA GGAGAAGGTG AAGCTTCTGA TTCAGCTGGC TGATAGCTTT
    GTGGAAAAAG GTCACATTCA TGCCACAGAG ATCAGGAAAT GGGTGACCAC AGTGGACAAG
    CATTACAGAG ACTTCTCACT GAGGATGGGA AAATACCGGT ACTCCCTGGA GAAGGCCCTA
    GGAGTCAACA CGGAGGATAA CAAGGACCTG GAGCTGGACA TCATCCCAGC AAGCCTTTCA
    GATCGTGAGG TCAAGCTCCG GGATGCCAAC CACGAGGTCA ATGAAGAGAA GCGGAAATCA
    GCCAGGAAAA AAGAATTCAT TATGGCAGAA TTGCTCCAGA CAGAGAAGGC TTATGTGAGG
    GACTTACATG AGTGCTTAGA GACCTACCTG TGGGAAATGA CCAGTGGTGT GGAGGAGATC
    CCCCCTGGGA TCCTTAATAA GGAGCATATC ATCTTTGGCA ACATCCAAGA GATCTACGAT
    TTTCATAACA ACATCTTCCT CAAAGAACTG GAGAAGTATG AGCAGCTGCC TGAGGATGTG
    GGACACTGCT TTGTTACTTG GGCAGACAAA TTTCAGATGT ACGTCACCTA CTGTAAAAAC
    AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCAGGCA CCTTCTTTGA TGAGATACAG
    CAGCGGCATG GTCTGGCCAA CTCCATTTCT TCCTACCTAA TTAAGCCTGT CCAGAGGATT
    ACCAAGTACC AGCTGCTCCT GAAGGAACTT TTAACTTGCT GTGAAGAAGG AAAAGGGGAG
    CTCAAGGAAG GCCTGGAGGT GATGCTTAGT GTCCCAAAGA GAGCCAACGA TGCCATGCAC
    GTCAGCATGC TGGAAGGGTT CGACGAGAAC CTGGACGTGC AGGGGGAGCT GATTTTACAA
    GATGCCTTTC AAGTGTGGGA CCCCAAGTCG CTGATCCGGA AAGGGCGGGA GCGACACCTA
    TTCCTCTTTG AGATCTCCTT GGTTTTCAGC AAGGAGATCA AAGACTCTTC AGGACACACA
    AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA ACACGTAGAG
    GGCGATCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA
    GTGCTGAAAG CCTCCAACAT AGAAACCAAG CAGGAGTGGA TCAAGAACAT TCGAGAGGTG
    ATTCAAGAAA GGATCATTCA CCTGAAAGGA GCTTTGAAGG AGCCAATTCA GCTCCCCAAA
    ACACCAGCCA AACAGAGGAA CAACAGTAAG AGGGATGGAG TGGAGGATGT TGACAGCCAG
    GGGGATGGGA GCAGCCAGCC AGACACCATC TCCATTGCCT CTAGGACCTC CCAGAACACA
    GCGGACAGTG ACAAGCTCTC TGGCGGCTGT GAGTTGACCG TGGTGCTCCA GGACTTCAAC
    GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGACAGA CGGTGGAGCT GCTAGAGCGG
    CCGAGCGAGC GGCCGGGCTG GTGTCTGGTC CGAACCACAG AGCGGAGCCC CCCCCAGGAG
    GGCCTGGTGC CCAGCAGCGC CCTGTGCATC TCCCACTCCC GAAGCAGCGT GGAGATGGAC
    TGCTTCTTCC CCCTGGTGAA AGATGCGTAC TCTCATTCCT CAAGTGAAAA CGGAGGCAAG
    TCCGAGTCGG TGGCCAACCT GCAGGCCCAA CCATCCCTGA ACTCCATCCA CAGCTCCCCT
    GGGCCCAAGC GTTCCACCAA CACCCTGAAG AAGTGGCTGA CGAGTCCCGT GCGCCGGCTT
    AACAGTGGGA AAGCAGATGG AAACATCAAG AAGCAGAAGA AAGTACGAGA TGGTCGGAAG
    AGCTTTGACC TGGGATCTCC CAAGCCTGGG GATGAGACGA CCCCTCAGGG AGACAGCGCT
    GATGAGAAGA GCAAGAAAGG TTGGGGTGAA GATGAGGCAG ATGAAGAGTC ACACACACCC
    CTCCCCCCGC CTATGAAGAT CTTTGACAAC GACCCCACGC AGGACGAAAT GAGTCTGGAA
    GGAGGCTCTT ACCGAGGGAG CTTGAAAGAC CCCACAGGCT GCCTGAATGA AGGCATGACC
    CCACCCACAC CTCCCAGAAA TCTGGAAGAA GAACAGAAAG CCAAGGCCCT GAGAGGCAGG
    ATGTTTGTCC TGAATGAACT GGTACAAACA GAGAAGGACT ATGTCAAGGA CCTGGGAATT
    GTGGTGGAGG GTTTCATGAA GAGAATAGAA GAAAAGGGTG TCCCCGAGGA CATGCGAGGA
    AAGGATAAAA TCGTGTTTGG AAATATTCAC CAGATTTATG ATTGGCATAA GGATGTTTTC
    CTGGGTGAAC TGGAAAAATG TATCCAGGAG CAAGACAGAT TGGCACAGCT CTTTATTAAA
    TATGAGCGGA AGCTGCACAT CTATGTGTGG TACTGCCAGA ACAAGCCGCG CTCCGAGTAC
    ATCGTCGCCG AGTACGACGC CTACTTTGAA GAGGTGAAAC AAGAGATAAA TCAAAGGCTG
    ACGCTTAGCG ACTTCCTTAT CAAGCCCATT CAGAGAATAA CAAAATATCA ATTGCTCCTC
    AAGGACTTCC TGAGATACAG CGAGAAGGCT GGTTTGGAGT GTTCAGATAT TGAGAAAGCA
    GTGGAGTTAA TGTGCCTTGT TCCAAAACGC TGCAATGACA TGATGAACCT GGGGCGCCTG
    CAGGGCTTTG AGGGGACCCT GACTGCTCAG GGCAAGCTGC TGCAGCAGGA CACATTCTAC
    GTGATTGAGC TGGACGCTGG CATGCAGTCG CGGACCAAGG AGAGGCGTGT GTTTCTCTTT
    GAGCAAATCG TCATCTTCAG TGAACTGCTC AGGAAGGGAT CCCTCACCCC AGGCTACATG
    TTCAAAAGGA GCATCAAGAT GAACTACTTG GTCCTGGAGG AGAACGTGGA CAATGACCCC
    TGCAAGTTTG CGCTTCTGAA CAGGGAGACT TCAGAGAGGG TTATTCTGCA AGCCGCCAAT
    GCTGACATCC AGCAGGCCTG GGTGCAGGAC ATCAACCAAG TCTTAGAAAC ACAGCGAGAC
    TTCTTAAATG CCCTACAGTC GCCCATTGAG TATCAGCGGA AAGAAAGGAG CACAGCCGTG
    ATGAGGTCCC AGCCTGCTAG GGTTCCCCAA GCCAGCCCCA GGCCCTACTC CTCCATCCCC
    GTGGGCTCCG AGAAGCCCCC AAAGGGCTCC AGCTATAACC CGCCTCTGCC ACCCCTGAAG
    ATATCTACCT CCAATGGCAG TCCAGGGTTT GACTACCACC AGGCTGGGGA CAAGTTTGAG
    GCCAGCAAGA ATGACCTGGG AGGCTGCAAC GGGGCCTCAT CCATGGCCGT GATCAAAGAT
    TATTATGCAC TGAAGGAGAA CGAAATCTGT GTGAGCCAAG GTGAGGTGGT CCAGGTCCTC
    GCCGTCAACC AGCAGAACAT GTGCCTGGTG TACCAGCCCG CTAGCGACCA CTCCCCCGCC
    GCTGAGGGCT GGGTCCCTGG CAGCATCCTG ACGCCCCTCA CCAAAGCCAT GGCAGCCACA
    GAAAATAGTG ACGGGAGCAT CAAGAAGTCA TGTTCATGGC ATACTCTACG CATGAGAAAG
    CGGGCAGAAG TGGAGAACAC GGGTAAAAAT GAAGCCACAG GGCCTCGTAA ACCCAAGGAT
    ATTCTGGGCA ACAAAGTCTC TGTTAAAGAG ACGAACAGTT CCGAGGAGTC AGAGTGTGAT
    GATCTTGACC CTAATACTAG CATGGAGATC TTAAATCCAA ATTTCATCCA AGAAGTGGCG
    CCAGAATTCC TCGTGCCCTT AGTGGATGTG ACCTGCTTGC TTGGGGACAC AGTGACTCTG
    CAGTGCAAAG TCTGTGGGCG GCCAAAGCCC ACCATCACTT GGAAGGGTCC AGACCAGAAC
    ATCCTTGACA CTGACAACAG CTCAGCTACA TACACCGTCT CCTCCTGTGA CTCTGGGGAA
    ATCACCCTGA AAATCTGCAA TCTGATGCCC CAAGACAGCG GTATCTATAC CTGCATAGCA
    GCAAACGACC ATGGTACTGC GTCAACATCT GCTACTGTTA AAGTGCAAGG TGTTCCAGCG
    GCCCCTAACC GCCCCATTGC CCAAGAGAGA AGCTGCACTT CCGTGATCCT CCGCTGGCTG
    CCCCCATCCA GCACGGGAAA CTGCACCATT TCCGGTTACA CCGTGGAGTA CAGAGAGGAA
    GGTTCCCAGG CCTGGCAGCA GTCAGTAGCA TCGACCTTGG ACACCTACCT TGTCATTGAA
    GACCTCAGTC CTGGGAGTCC TTATCAGTTC AGAGTCAGTG CCAGTAACCC CTGGGGGATC
    AGCCTGCCCA GCGAGCCCTC AGAATTCGTG CGACTTCCAG ATTACGACGC TGCTGCCGAT
    GGTGCCACCA TTTCTTGGAA GGAAAATTTT GATTCAGCTT ATACTGAGCT GAATGAAATT
    GGAAGAGGCC GGTTCTCCAT AGTAAAGAAG TGCATTCACA AAGCTACTCG CAAAGATGTC
    GCTGTGAAAT TTGTTAGCAA AAAAATGAAG AAAAAAGAGC AGGCTGCCCA CGAGGCTGCC
    CTGCTTCAGC ACCTGCAGCA CCCCCAATAC ATCACTCTCC ATGACACCTA TGAGTCCCCC
    ACTTCCTACA TCCTCATTTT GGAACTGATG GACGATGGCC GACTCTTAGA CTATCTTATG
    AATCATGATG AGCTGATGGA AGAAAAAGTA GCTTTCTACA TTCGAGACAT CATGGAAGCC
    CTGCAGTACC TTCATAACTG CAGAGTTGCA CATTTGGACA TAAAGCCTGA AAACCTGCTC
    ATTGACCTAC GGATTCCAGT GCCTCGAGTG AAGCTCATTG ACTTGGAGGA TGCTGTCCAG
    ATCTCAGGTC ACTTCCATAT CCACCACCTT CTGGGGAACC CCGAGTTTGC TGCCCCAGAA
    GTGATCCAGG GCATCCCTGT ATCCCTGGGC ACCGACATCT GGAGCATCGG GGTTTTGACA
    TACGTCATGC TGAGTGGGGT CTCCCCCTTC TTGGATGAGA GCAAGGAGGA GACATGTATC
    AATGTGTGCA GGGTGGACTT CAGCTTCCCC CATGAGTACT TCTGCGGTGT GAGCAATGCT
    GCCAGAGATT TCATCAATGT GATCCTACAG GAAGACTTTC GGAGGCGGCC CACAGCAGCC
    ACCTGCCTGC AGCATCCGTG GCTGCAGCCC CACAATGGCA GCTACTCCAA GATCCCCCTG
    GACACCTCCC GCTTGGCGTG CTTCATCGAG CGCCGCAAGC ATCAGAATGA TGTGCGCCCC
    ATTCCCAACG TCAAGAGCTA CATTGTCAAT CGGCTAAACC AAGGCACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847129.1
    CDS359..9229
    Translation

    Target ORF information:

    RefSeq Version XM_024991361.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991361.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CACACCAGAG CGCCCTGCAG
    GTTCAGCAGA AAGCTGAGGC GCTGCTCCAG GCTGGTCACT ATGATGCAGA TGCCATCCGG
    GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAGCAACTCA TGCTCAAGAT GGAAGACCGA
    CTGAAACTGG TCAATGCCTC AGTGGCCTTT TATAAAACTT CTGAGCAGGT CTGTAGTGTC
    CTGGAGAGCT TAGAGCAAGA ATACCGAAGA GATGAGGACT GGTGTGGTGG ACGAGATAAA
    TTGGGGCCAG CAGCAGAGAT CGACCACGTC ATTCCACTCA TCAGTAAACA TTTGGAACAA
    AAGGAAGCCT TTCTTAAGGC CTGCACCCTG GCACGGCGGA ATGCTGAGGT CTTTCTCAAG
    TACATCCATA GGAACAACGT CAGCATGCCC AGTGCTGCCA GCCACACCCG AGGACCCGAA
    CAGCAAGTGA AAGCCATCCT GAGTGAGCTC TTACAGAGGG AGAACCGTGT CCTGCACTTC
    TGGACATTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ACGTGGTATT TGAGCGAAGC
    GCCAAGCAGG CACTAGACTG GATCCAAGAA ACAGGTGAAT ATTACCTCTC AACACATACC
    TCCACCGGGG AGACCACAGA GGAGACTCAG GAACTGCTGA AAGAATATGG GGAGTTCAGG
    GTGCCTGCCA AGCAAACAAA GGAGAAGGTG AAGCTTCTGA TTCAGCTGGC TGATAGCTTT
    GTGGAAAAAG GTCACATTCA TGCCACAGAG ATCAGGAAAT GGGTGACCAC AGTGGACAAG
    CATTACAGAG ACTTCTCACT GAGGATGGGA AAATACCGGT ACTCCCTGGA GAAGGCCCTA
    GGAGTCAACA CGGAGGATAA CAAGGACCTG GAGCTGGACA TCATCCCAGC AAGCCTTTCA
    GATCGTGAGG TCAAGCTCCG GGATGCCAAC CACGAGGTCA ATGAAGAGAA GCGGAAATCA
    GCCAGGAAAA AAGAATTCAT TATGGCAGAA TTGCTCCAGA CAGAGAAGGC TTATGTGAGG
    GACTTACATG AGTGCTTAGA GACCTACCTG TGGGAAATGA CCAGTGGTGT GGAGGAGATC
    CCCCCTGGGA TCCTTAATAA GGAGCATATC ATCTTTGGCA ACATCCAAGA GATCTACGAT
    TTTCATAACA ACATCTTCCT CAAAGAACTG GAGAAGTATG AGCAGCTGCC TGAGGATGTG
    GGACACTGCT TTGTTACTTG GGCAGACAAA TTTCAGATGT ACGTCACCTA CTGTAAAAAC
    AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCAGGCA CCTTCTTTGA TGAGATACAG
    CAGCGGCATG GTCTGGCCAA CTCCATTTCT TCCTACCTAA TTAAGCCTGT CCAGAGGATT
    ACCAAGTACC AGCTGCTCCT GAAGGAACTT TTAACTTGCT GTGAAGAAGG AAAAGGGGAG
    CTCAAGGAAG GCCTGGAGGT GATGCTTAGT GTCCCAAAGA GAGCCAACGA TGCCATGCAC
    GTCAGCATGC TGGAAGGGTT CGACGAGAAC CTGGACGTGC AGGGGGAGCT GATTTTACAA
    GATGCCTTTC AAGTGTGGGA CCCCAAGTCG CTGATCCGGA AAGGGCGGGA GCGACACCTA
    TTCCTCTTTG AGATCTCCTT GGTTTTCAGC AAGGAGATCA AAGACTCTTC AGGACACACA
    AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA ACACGTAGAG
    GGCGATCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA
    GTGCTGAAAG CCTCCAACAT AGAAACCAAG CAGGAGTGGA TCAAGAACAT TCGAGAGGTG
    ATTCAAGAAA GGATCATTCA CCTGAAAGGA GCTTTGAAGG AGCCAATTCA GCTCCCCAAA
    ACACCAGCCA AACAGAGGAA CAACAGTAAG AGGGATGGAG TGGAGGATGT TGACAGCCAG
    GGGGATGGGA GCAGCCAGCC AGACACCATC TCCATTGCCT CTAGGACCTC CCAGAACACA
    GCGGACAGTG ACAAGCTCTC TGGCGGCTGT GAGTTGACCG TGGTGCTCCA GGACTTCAAC
    GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGACAGA CGGTGGAGCT GCTAGAGCGG
    CCGAGCGAGC GGCCGGGCTG GTGTCTGGTC CGAACCACAG AGCGGAGCCC CCCCCAGGAG
    GGCCTGGTGC CCAGCAGCGC CCTGTGCATC TCCCACTCCC GAAGCAGCGT GGAGATGGAC
    TGCTTCTTCC CCCTGGTGAA AGATGCGTAC TCTCATTCCT CAAGTGAAAA CGGAGGCAAG
    TCCGAGTCGG TGGCCAACCT GCAGGCCCAA CCATCCCTGA ACTCCATCCA CAGCTCCCCT
    GGGCCCAAGC GTTCCACCAA CACCCTGAAG AAGTGGCTGA CGAGTCCCGT GCGCCGGCTT
    AACAGTGGGA AAGCAGATGG AAACATCAAG AAGCAGAAGA AAGTACGAGA TGGTCGGAAG
    AGCTTTGACC TGGGATCTCC CAAGCCTGGG GATGAGACGA CCCCTCAGGG AGACAGCGCT
    GATGAGAAGA GCAAGAAAGG TTGGGGTGAA GATGAGGCAG ATGAAGAGTC ACACACACCC
    CTCCCCCCGC CTATGAAGAT CTTTGACAAC GACCCCACGC AGGACGAAAT GAGTCTGGAA
    GGAGGCTCTT ACCGAGGGAG CTTGAAAGAC CCCACAGGCT GCCTGAATGA AGGCATGACC
    CCACCCACAC CTCCCAGAAA TCTGGAAGAA GAACAGAAAG CCAAGGCCCT GAGAGGCAGG
    ATGTTTGTCC TGAATGAACT GGTACAAACA GAGAAGGACT ATGTCAAGGA CCTGGGAATT
    GTGGTGGAGG GTTTCATGAA GAGAATAGAA GAAAAGGGTG TCCCCGAGGA CATGCGAGGA
    AAGGATAAAA TCGTGTTTGG AAATATTCAC CAGATTTATG ATTGGCATAA GGATGTTTTC
    CTGGGTGAAC TGGAAAAATG TATCCAGGAG CAAGACAGAT TGGCACAGCT CTTTATTAAA
    TATGAGCGGA AGCTGCACAT CTATGTGTGG TACTGCCAGA ACAAGCCGCG CTCCGAGTAC
    ATCGTCGCCG AGTACGACGC CTACTTTGAA GAGGTGAAAC AAGAGATAAA TCAAAGGCTG
    ACGCTTAGCG ACTTCCTTAT CAAGCCCATT CAGAGAATAA CAAAATATCA ATTGCTCCTC
    AAGGACTTCC TGAGATACAG CGAGAAGGCT GGTTTGGAGT GTTCAGATAT TGAGAAAGCA
    GTGGAGTTAA TGTGCCTTGT TCCAAAACGC TGCAATGACA TGATGAACCT GGGGCGCCTG
    CAGGGCTTTG AGGGGACCCT GACTGCTCAG GGCAAGCTGC TGCAGCAGGA CACATTCTAC
    GTGATTGAGC TGGACGCTGG CATGCAGTCG CGGACCAAGG AGAGGCGTGT GTTTCTCTTT
    GAGCAAATCG TCATCTTCAG TGAACTGCTC AGGAAGGGAT CCCTCACCCC AGGCTACATG
    TTCAAAAGGA GCATCAAGAT GAACTACTTG GTCCTGGAGG AGAACGTGGA CAATGACCCC
    TGCAAGTTTG CGCTTCTGAA CAGGGAGACT TCAGAGAGGG TTATTCTGCA AGCCGCCAAT
    GCTGACATCC AGCAGGCCTG GGTGCAGGAC ATCAACCAAG TCTTAGAAAC ACAGCGAGAC
    TTCTTAAATG CCCTACAGTC GCCCATTGAG TATCAGCGGA AAGAAAGGAG CACAGCCGTG
    ATGAGGTCCC AGCCTGCTAG GGTTCCCCAA GCCAGCCCCA GGCCCTACTC CTCCATCCCC
    GTGGGCTCCG AGAAGCCCCC AAAGGGCTCC AGCTATAACC CGCCTCTGCC ACCCCTGAAG
    ATATCTACCT CCAATGGCAG TCCAGGGTTT GACTACCACC AGGCTGGGGA CAAGTTTGAG
    GCCAGCAAGA ATGACCTGGG AGGCTGCAAC GGGGCCTCAT CCATGGCCGT GATCAAAGAT
    TATTATGCAC TGAAGGAGAA CGAAATCTGT GTGAGCCAAG GTGAGGTGGT CCAGGTCCTC
    GCCGTCAACC AGCAGAACAT GTGCCTGGTG TACCAGCCCG CTAGCGACCA CTCCCCCGCC
    GCTGAGGGCT GGGTCCCTGG CAGCATCCTG ACGCCCCTCA CCAAAGCCAT GGCAGCCACA
    GAAAATAGTG ACGGGAGCAT CAAGAAGTCA TGTTCATGGC ATACTCTACG CATGAGAAAG
    CGGGCAGAAG TGGAGAACAC GGGTAAAAAT GAAGCCACAG GGCCTCGTAA ACCCAAGGAT
    ATTCTGGGCA ACAAAGTCTC TGTTAAAGAG ACGAACAGTT CCGAGGAGTC AGAGTGTGAT
    GATCTTGACC CTAATACTAG CATGGAGATC TTAAATCCAA ATTTCATCCA AGAAGTGGCG
    CCAGAATTCC TCGTGCCCTT AGTGGATGTG ACCTGCTTGC TTGGGGACAC AGTGACTCTG
    CAGTGCAAAG TCTGTGGGCG GCCAAAGCCC ACCATCACTT GGAAGGGTCC AGACCAGAAC
    ATCCTTGACA CTGACAACAG CTCAGCTACA TACACCGTCT CCTCCTGTGA CTCTGGGGAA
    ATCACCCTGA AAATCTGCAA TCTGATGCCC CAAGACAGCG GTATCTATAC CTGCATAGCA
    GCAAACGACC ATGGTACTGC GTCAACATCT GCTACTGTTA AAGTGCAAGG TGTTCCAGCG
    GCCCCTAACC GCCCCATTGC CCAAGAGAGA AGCTGCACTT CCGTGATCCT CCGCTGGCTG
    CCCCCATCCA GCACGGGAAA CTGCACCATT TCCGGTTACA CCGTGGAGTA CAGAGAGGAA
    GGTTCCCAGG CCTGGCAGCA GTCAGTAGCA TCGACCTTGG ACACCTACCT TGTCATTGAA
    GACCTCAGTC CTGGGAGTCC TTATCAGTTC AGAGTCAGTG CCAGTAACCC CTGGGGGATC
    AGCCTGCCCA GCGAGCCCTC AGAATTCGTG CGACTTCCAG ATTACGACGC TGCTGCCGAT
    GGTGCCACCA TTTCTTGGAA GGAAAATTTT GATTCAGCTT ATACTGAGCT GAATGAAATT
    GGAAGAGGCC GGTTCTCCAT AGTAAAGAAG TGCATTCACA AAGCTACTCG CAAAGATGTC
    GCTGTGAAAT TTGTTAGCAA AAAAATGAAG AAAAAAGAGC AGGCTGCCCA CGAGGCTGCC
    CTGCTTCAGC ACCTGCAGCA CCCCCAATAC ATCACTCTCC ATGACACCTA TGAGTCCCCC
    ACTTCCTACA TCCTCATTTT GGAACTGATG GACGATGGCC GACTCTTAGA CTATCTTATG
    AATCATGATG AGCTGATGGA AGAAAAAGTA GCTTTCTACA TTCGAGACAT CATGGAAGCC
    CTGCAGTACC TTCATAACTG CAGAGTTGCA CATTTGGACA TAAAGCCTGA AAACCTGCTC
    ATTGACCTAC GGATTCCAGT GCCTCGAGTG AAGCTCATTG ACTTGGAGGA TGCTGTCCAG
    ATCTCAGGTC ACTTCCATAT CCACCACCTT CTGGGGAACC CCGAGTTTGC TGCCCCAGAA
    GTGATCCAGG GCATCCCTGT ATCCCTGGGC ACCGACATCT GGAGCATCGG GGTTTTGACA
    TACGTCATGC TGAGTGGGGT CTCCCCCTTC TTGGATGAGA GCAAGGAGGA GACATGTATC
    AATGTGTGCA GGGTGGACTT CAGCTTCCCC CATGAGTACT TCTGCGGTGT GAGCAATGCT
    GCCAGAGATT TCATCAATGT GATCCTACAG GAAGACTTTC GGAGGCGGCC CACAGCAGCC
    ACCTGCCTGC AGCATCCGTG GCTGCAGCCC CACAATGGCA GCTACTCCAA GATCCCCCTG
    GACACCTCCC GCTTGGCGTG CTTCATCGAG CGCCGCAAGC ATCAGAATGA TGTGCGCCCC
    ATTCCCAACG TCAAGAGCTA CATTGTCAAT CGGCTAAACC AAGGCACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218893
    Clone ID Related Accession (Same CDS sequence) XM_024991366.1
    Accession Version XM_024991366.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kalirin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CACACCAGAG CGCCCTGCAG
    GTTCAGCAGA AAGCTGAGGC GCTGCTCCAG GCTGGTCACT ATGATGCAGA TGCCATCCGG
    GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAGCAACTCA TGCTCAAGAT GGAAGACCGA
    CTGAAACTGG TCAATGCCTC AGTGGCCTTT TATAAAACTT CTGAGCAGGT CTGTAGTGTC
    CTGGAGAGCT TAGAGCAAGA ATACCGAAGA GATGAGGACT GGTGTGGTGG ACGAGATAAA
    TTGGGGCCAG CAGCAGAGAT CGACCACGTC ATTCCACTCA TCAGTAAACA TTTGGAACAA
    AAGGAAGCCT TTCTTAAGGC CTGCACCCTG GCACGGCGGA ATGCTGAGGT CTTTCTCAAG
    TACATCCATA GGAACAACGT CAGCATGCCC AGTGCTGCCA GCCACACCCG AGGACCCGAA
    CAGCAAGTGA AAGCCATCCT GAGTGAGCTC TTACAGAGGG AGAACCGTGT CCTGCACTTC
    TGGACATTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ACGTGGTATT TGAGCGAAGC
    GCCAAGCAGG CACTAGACTG GATCCAAGAA ACAGGTGAAT ATTACCTCTC AACACATACC
    TCCACCGGGG AGACCACAGA GGAGACTCAG GAACTGCTGA AAGAATATGG GGAGTTCAGG
    GTGCCTGCCA AGCAAACAAA GGAGAAGGTG AAGCTTCTGA TTCAGCTGGC TGATAGCTTT
    GTGGAAAAAG GTCACATTCA TGCCACAGAG ATCAGGAAAT GGGTGACCAC AGTGGACAAG
    CATTACAGAG ACTTCTCACT GAGGATGGGA AAATACCGGT ACTCCCTGGA GAAGGCCCTA
    GGAGTCAACA CGGAGGATAA CAAGGACCTG GAGCTGGACA TCATCCCAGC AAGCCTTTCA
    GATCGTGAGG TCAAGCTCCG GGATGCCAAC CACGAGGTCA ATGAAGAGAA GCGGAAATCA
    GCCAGGAAAA AAGAATTCAT TATGGCAGAA TTGCTCCAGA CAGAGAAGGC TTATGTGAGG
    GACTTACATG AGTGCTTAGA GACCTACCTG TGGGAAATGA CCAGTGGTGT GGAGGAGATC
    CCCCCTGGGA TCCTTAATAA GGAGCATATC ATCTTTGGCA ACATCCAAGA GATCTACGAT
    TTTCATAACA ACATCTTCCT CAAAGAACTG GAGAAGTATG AGCAGCTGCC TGAGGATGTG
    GGACACTGCT TTGTTACTTG GGCAGACAAA TTTCAGATGT ACGTCACCTA CTGTAAAAAC
    AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCAGGCA CCTTCTTTGA TGAGATACAG
    CAGCGGCATG GTCTGGCCAA CTCCATTTCT TCCTACCTAA TTAAGCCTGT CCAGAGGATT
    ACCAAGTACC AGCTGCTCCT GAAGGAACTT TTAACTTGCT GTGAAGAAGG AAAAGGGGAG
    CTCAAGGAAG GCCTGGAGGT GATGCTTAGT GTCCCAAAGA GAGCCAACGA TGCCATGCAC
    GTCAGCATGC TGGAAGGGTT CGACGAGAAC CTGGACGTGC AGGGGGAGCT GATTTTACAA
    GATGCCTTTC AAGTGTGGGA CCCCAAGTCG CTGATCCGGA AAGGGCGGGA GCGACACCTA
    TTCCTCTTTG AGATCTCCTT GGTTTTCAGC AAGGAGATCA AAGACTCTTC AGGACACACA
    AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA ACACGTAGAG
    GGCGATCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA
    GTGCTGAAAG CCTCCAACAT AGAAACCAAG CAGGAGTGGA TCAAGAACAT TCGAGAGGTG
    ATTCAAGAAA GGATCATTCA CCTGAAAGGA GCTTTGAAGG AGCCAATTCA GCTCCCCAAA
    ACACCAGCCA AACAGAGGAA CAACAGTAAG AGGGATGGAG TGGAGGATGT TGACAGCCAG
    GGGGATGGGA GCAGCCAGCC AGACACCATC TCCATTGCCT CTAGGACCTC CCAGAACACA
    GCGGACAGTG ACAAGCTCTC TGGCGGCTGT GAGTTGACCG TGGTGCTCCA GGACTTCAAC
    GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGACAGA CGGTGGAGCT GCTAGAGCGG
    CCGAGCGAGC GGCCGGGCTG GTGTCTGGTC CGAACCACAG AGCGGAGCCC CCCCCAGGAG
    GGCCTGGTGC CCAGCAGCGC CCTGTGCATC TCCCACTCCC GAAGCAGCGT GGAGATGGAC
    TGCTTCTTCC CCCTGGTGAA AGATGCGTAC TCTCATTCCT CAAGTGAAAA CGGAGGCAAG
    TCCGAGTCGG TGGCCAACCT GCAGGCCCAA CCATCCCTGA ACTCCATCCA CAGCTCCCCT
    GGGCCCAAGC GTTCCACCAA CACCCTGAAG AAGTGGCTGA CGAGTCCCGT GCGCCGGCTT
    AACAGTGGGA AAGCAGATGG AAACATCAAG AAGCAGAAGA AAGTACGAGA TGGTCGGAAG
    AGCTTTGACC TGGGATCTCC CAAGCCTGGG GATGAGACGA CCCCTCAGGG AGACAGCGCT
    GATGAGAGCA AGAAAGGTTG GGGTGAAGAT GAGGCAGATG AAGAGTCACA CACACCCCTC
    CCCCCGCCTA TGAAGATCTT TGACAACGAC CCCACGCAGG ACGAAATGAG TCTGGAAGGA
    GGCTCTTACC GAGGGAGCTT GAAAGACCCC ACAGGCTGCC TGAATGAAGG CATGACCCCA
    CCCACACCTC CCAGAAATCT GGAAGAAGAA CAGAAAGCCA AGGCCCTGAG AGGCAGGATG
    TTTGTCCTGA ATGAACTGGT ACAAACAGAG AAGGACTATG TCAAGGACCT GGGAATTGTG
    GTGGAGGGTT TCATGAAGAG AATAGAAGAA AAGGGTGTCC CCGAGGACAT GCGAGGAAAG
    GATAAAATCG TGTTTGGAAA TATTCACCAG ATTTATGATT GGCATAAGGA TGTTTTCCTG
    GGTGAACTGG AAAAATGTAT CCAGGAGCAA GACAGATTGG CACAGCTCTT TATTAAATAT
    GAGCGGAAGC TGCACATCTA TGTGTGGTAC TGCCAGAACA AGCCGCGCTC CGAGTACATC
    GTCGCCGAGT ACGACGCCTA CTTTGAAGAG GTGAAACAAG AGATAAATCA AAGGCTGACG
    CTTAGCGACT TCCTTATCAA GCCCATTCAG AGAATAACAA AATATCAATT GCTCCTCAAG
    GACTTCCTGA GATACAGCGA GAAGGCTGGT TTGGAGTGTT CAGATATTGA GAAAGCAGTG
    GAGTTAATGT GCCTTGTTCC AAAACGCTGC AATGACATGA TGAACCTGGG GCGCCTGCAG
    GGCTTTGAGG GGACCCTGAC TGCTCAGGGC AAGCTGCTGC AGCAGGACAC ATTCTACGTG
    ATTGAGCTGG ACGCTGGCAT GCAGTCGCGG ACCAAGGAGA GGCGTGTGTT TCTCTTTGAG
    CAAATCGTCA TCTTCAGTGA ACTGCTCAGG AAGGGATCCC TCACCCCAGG CTACATGTTC
    AAAAGGAGCA TCAAGATGAA CTACTTGGTC CTGGAGGAGA ACGTGGACAA TGACCCCTGC
    AAGTTTGCGC TTCTGAACAG GGAGACTTCA GAGAGGGTTA TTCTGCAAGC CGCCAATGCT
    GACATCCAGC AGGCCTGGGT GCAGGACATC AACCAAGTCT TAGAAACACA GCGAGACTTC
    TTAAATGCCC TACAGTCGCC CATTGAGTAT CAGCGGAAAG AAAGGAGCAC AGCCGTGATG
    AGGTCCCAGC CTGCTAGGGT TCCCCAAGCC AGCCCCAGGC CCTACTCCTC CATCCCCGTG
    GGCTCCGAGA AGCCCCCAAA GGGCTCCAGC TATAACCCGC CTCTGCCACC CCTGAAGATA
    TCTACCTCCA ATGGCAGTCC AGGGTTTGAC TACCACCAGG CTGGGGACAA GTTTGAGGCC
    AGCAAGAATG ACCTGGGAGG CTGCAACGGG GCCTCATCCA TGGCCGTGAT CAAAGATTAT
    TATGCACTGA AGGAGAACGA AATCTGTGTG AGCCAAGGTG AGGTGGTCCA GGTCCTCGCC
    GTCAACCAGC AGAACATGTG CCTGGTGTAC CAGCCCGCTA GCGACCACTC CCCCGCCGCT
    GAGGGCTGGG TCCCTGGCAG CATCCTGACG CCCCTCACCA AAGCCATGGC AGCCACAGAA
    AATAGTGACG GGAGCATCAA GAAGTCATGT TCATGGCATA CTCTACGCAT GAGAAAGCGG
    GCAGAAGTGG AGAACACGGG TAAAAATGAA GCCACAGGGC CTCGTAAACC CAAGGATATT
    CTGGGCAACA AAGTCTCTGT TAAAGAGACG AACAGTTCCG AGGAGTCAGA GTGTGATGAT
    CTTGACCCTA ATACTAGCAT GGAGATCTTA AATCCAAATT TCATCCAAGA AGTGGCGCCA
    GAATTCCTCG TGCCCTTAGT GGATGTGACC TGCTTGCTTG GGGACACAGT GACTCTGCAG
    TGCAAAGTCT GTGGGCGGCC AAAGCCCACC ATCACTTGGA AGGGTCCAGA CCAGAACATC
    CTTGACACTG ACAACAGCTC AGCTACATAC ACCGTCTCCT CCTGTGACTC TGGGGAAATC
    ACCCTGAAAA TCTGCAATCT GATGCCCCAA GACAGCGGTA TCTATACCTG CATAGCAGCA
    AACGACCATG GTACTGCGTC AACATCTGCT ACTGTTAAAG TGCAAGGTGT TCCAGCGGCC
    CCTAACCGCC CCATTGCCCA AGAGAGAAGC TGCACTTCCG TGATCCTCCG CTGGCTGCCC
    CCATCCAGCA CGGGAAACTG CACCATTTCC GGTTACACCG TGGAGTACAG AGAGGAAGGT
    TCCCAGGCCT GGCAGCAGTC AGTAGCATCG ACCTTGGACA CCTACCTTGT CATTGAAGAC
    CTCAGTCCTG GGAGTCCTTA TCAGTTCAGA GTCAGTGCCA GTAACCCCTG GGGGATCAGC
    CTGCCCAGCG AGCCCTCAGA ATTCGTGCGA CTTCCAGATT ACGACGCTGC TGCCGATGGT
    GCCACCATTT CTTGGAAGGA AAATTTTGAT TCAGCTTATA CTGAGCTGAA TGAAATTGGA
    AGAGGCCGGT TCTCCATAGT AAAGAAGTGC ATTCACAAAG CTACTCGCAA AGATGTCGCT
    GTGAAATTTG TTAGCAAAAA AATGAAGAAA AAAGAGCAGG CTGCCCACGA GGCTGCCCTG
    CTTCAGCACC TGCAGCACCC CCAATACATC ACTCTCCATG ACACCTATGA GTCCCCCACT
    TCCTACATCC TCATTTTGGA ACTGATGGAC GATGGCCGAC TCTTAGACTA TCTTATGAAT
    CATGATGAGC TGATGGAAGA AAAAGTAGCT TTCTACATTC GAGACATCAT GGAAGCCCTG
    CAGTACCTTC ATAACTGCAG AGTTGCACAT TTGGACATAA AGCCTGAAAA CCTGCTCATT
    GACCTACGGA TTCCAGTGCC TCGAGTGAAG CTCATTGACT TGGAGGATGC TGTCCAGATC
    TCAGGTCACT TCCATATCCA CCACCTTCTG GGGAACCCCG AGTTTGCTGC CCCAGAAGTG
    ATCCAGGGCA TCCCTGTATC CCTGGGCACC GACATCTGGA GCATCGGGGT TTTGACATAC
    GTCATGCTGA GTGGGGTCTC CCCCTTCTTG GATGAGAGCA AGGAGGAGAC ATGTATCAAT
    GTGTGCAGGG TGGACTTCAG CTTCCCCCAT GAGTACTTCT GCGGTGTGAG CAATGCTGCC
    AGAGATTTCA TCAATGTGAT CCTACAGGAA GACTTTCGGA GGCGGCCCAC AGCAGCCACC
    TGCCTGCAGC ATCCGTGGCT GCAGCCCCAC AATGGCAGCT ACTCCAAGAT CCCCCTGGAC
    ACCTCCCGCT TGGCGTGCTT CATCGAGCGC CGCAAGCATC AGAATGATGT GCGCCCCATT
    CCCAACGTCA AGAGCTACAT TGTCAATCGG CTAAACCAAG GCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847134.1
    CDS359..9226
    Translation

    Target ORF information:

    RefSeq Version XM_024991366.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kalirin RhoGEF kinase (KALRN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991366.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    ATGAACCCCC CGGAGGGGGC AGCGGAGGAA GGAGGAGCCG CCGACTCGGA CGTGGACGCC 
    TTTTTCAGGA CAGGCTCTTT TCGGAATGAT GGTTTGAAAG CTTCTGATGT CCTTCCCATC
    CTAAAGGAAA AAGTGGCCTT CGTGTCTGGG GGCCGTGATA AGCGAGGTGG ACCCATCCTG
    ACCTTCCCTG CTCGCAGCAA TCATGACAGA ATAAGACAGG AAGACCTGCG GAAACTGGTG
    ACCTATCTGG CCAGCGTGCC AAGCGAGGAC GTGTGCAAAC GTGGCTTCAC TGTCATCATC
    GATATGCGGG GGTCCAAGTG GGACCTCATC AAGCCCCTCC TCAAGACGCT GCAAGAAGCC
    TTTCCCGCTG AGATCCATGT GGCCCTCATC ATCAAACCCG ACAACTTCTG GCAGAAACAG
    AAGACCAACT TTGGCAGCTC CAAGTTCATC TTTGAGACAA GCATGGTGTC CGTGGAGGGC
    CTCACGAAGC TGGTGGACCC CTCCCAGCTG ACGGAGGAGT TCGATGGCTC CCTGGACTAC
    AACCACGAGG AGTGGATCGA GCTGCGGCTC TCCCTGGAGG AGTTCTTCAA CAGCGCCGTA
    CACCTGCTCT CACGCCTTGA AGACCTGCAG GAGATGCTGG CCCGGAAGGA GTTCCCCGTG
    GATGTGGAGG GCTCGAGGCG GCTAATTGAT GAACACACAC AGCTGAAGAA GAAGGTGCTG
    AAGGCGCCTG TGGAGGAGCT GGACCGGGAG GGGCAGCGAC TGCTGCAGTG CATCCGCTGC
    AGTGACGGCT TCTCAGGGCG CAACTGCATC CCGGGCAGTG CCGACTTCCA GAGCTTGGTG
    CCCAAGATCA CCAGCCTGTT GGACAAGCTG CACTCCACCC GGCAGCACCT GCACCAGATG
    TGGCATGTGC GCAAGCTCAA GCTAGACCAG TGCTTCCAGC TGCGGCTCTT CGAGCAGGAT
    GCTGAAAAGA TGTTTGACTG GATAAGCCAC AACAAGGAGT TATTCCTCCA GAGCCATACA
    GAGATTGGAG TCAGCTACCA GCACGCCCTA GACCTCCAGA CACAGCACAA TCACTTTGCC
    ATGAACTCCA TGAATGCCTA TGTCAACATC AACCGCATCA TGTCTGTGGC TTCCCGCCTC
    TCAGAGGCTG GCCACTATGC TTCACAGCAA ATCAAGCAGA TCTCCACGCA GCTAGACCAG
    GAGTGGAAGA GTTTCGCAGC TGCCCTGGAT GAACGCAGCA CCATCCTCGC CATGTCTGCT
    GTGTTCCATC AGAAGGCTGA GCAGTTCTTG TCAGGAGTGG ATGCCTGGTG CAAGATGTGC
    AGTGAAGGTG GCCTGCCCTC TGAGATGCAG GACCTGGAGC TGGCGATCCA CCACCACCAG
    TCTTTGTATG AGCAGGTGAC CCAGGCCTAC ACAGAGGTCA GCCAGGACGG CAAAGCACTG
    CTGGATGTGC TGCAGCGGCC CCTGAGTCCC GGGAACTCTG AGTCCCTCAC AGCCACAGCC
    AACTACTCCA AGGCGGTGCA CCAGGTGCTG GACGTGGTGC ACGAAGTGCT GCACCACCAG
    CGGCGGCTCG AGAGCATCTG GCAGCACCGC AAGGTGCGGC TCCACCAACG CCTGCAGCTC
    TGCGTCTTCC AGCAGGACGT CCAGCAGGTG TTAGATTGGA TTGAAAACCA CGGCGAGGCC
    TTTCTCAGCA AGCACACAGG GGTCGGGAAG TCTCTGCACC GAGCTCGGGC CCTGCAGAAG
    AGGCACGATG ACTTTGAAGA GGTGGCTCAG AATACATACA CCAATGCGGA CAAGCTCCTA
    GAAGCGGCAG AGCAGCTGGC TCAGACAGGG GAATGTGACC CGGAGGAGAT CTACAAGGCA
    GCTAGACACC TGGAGGTGCG CATCCAAGAC TTCGTGCGCA GGGTGGAGCA GCGGAAGCTT
    CTGCTGGACA TGTCTGTCTC CTTCCATACT CACACCAAAG AGCTATGGAC ATGGATGGAA
    GACCTTCAGA AGGAGATGCT GGAGGATGTC TGTGCAGACT CTGTGGATGC GGTCCAGGAA
    CTGATCAAGC AGTTCCAGCA GCAGCAGACC GCCACTCTGG ATGCCACACT TAATGTCATC
    AAGGAGGGCG AAGACCTTAT CCAGCAACTC AGGTCAGCGC CTCCCTCCCT GGGGGAGCCC
    AGCGAGGCCA GGGACTCAGC TGTGTCCAAC AACAAGACGC CCCATAGTAG CTCCATCAGC
    CACATAGAGT CGGTGCTGCA GCAGCTGGAT GACGCCCAGG TGCAAATGGA GGAGCTGTTC
    CACGAACGCA AGATCAAGCT GGACATCTTC CTGCAGCTGC GCATCTTTGA GCAGTACACC
    ATTGAGGTGA CAGCAGAGCT CGACGCTTGG AATGAGGATT TGCTTCGGCA GATGAATGAC
    TTCAACACGG AGGATCTAAC ACTGGCGGAA CAGCGGCTGC AGCGCCACAC AGAGCGGAAG
    CTAGCCATGA ACAACATGAC CTTTGAGGTC ATCCAGCAAG GCCAGGATCT GCACCAGTAC
    ATCATGGAGG TCCAGGCCTC AGGGATTGAG TTAATCTGTG AAAAAGACAT TGATCTGGCA
    GCCCAGGTAC AAGAGCTATT AGAGTTTCTC CATGAGAAAC AGCATGAGTT GGAGCTCAAC
    GCAGAGCAGA CCCACAAGCG ACTTGAGCAG TGCCTCCAAC TGCGGCACCT CCAGGCCGAG
    GTCAAACAGG TCCTAGGATG GATCCGCAAT GGGGAGTCAA TGCTCAATGC CAGCCTGGTC
    AACGCCAGCT CTTTGTCTGA AGCAGAGCAG CTACAGCGGG AGCACGAGCA GTTCCAACTA
    GCCATCGAGT CCCTCTTTCA TGCCACTTCC TTGCAGAAGA CACACCAGAG CGCCCTGCAG
    GTTCAGCAGA AAGCTGAGGC GCTGCTCCAG GCTGGTCACT ATGATGCAGA TGCCATCCGG
    GAATGTGCTG AGAAGGTGGC CCTCCACTGG CAGCAACTCA TGCTCAAGAT GGAAGACCGA
    CTGAAACTGG TCAATGCCTC AGTGGCCTTT TATAAAACTT CTGAGCAGGT CTGTAGTGTC
    CTGGAGAGCT TAGAGCAAGA ATACCGAAGA GATGAGGACT GGTGTGGTGG ACGAGATAAA
    TTGGGGCCAG CAGCAGAGAT CGACCACGTC ATTCCACTCA TCAGTAAACA TTTGGAACAA
    AAGGAAGCCT TTCTTAAGGC CTGCACCCTG GCACGGCGGA ATGCTGAGGT CTTTCTCAAG
    TACATCCATA GGAACAACGT CAGCATGCCC AGTGCTGCCA GCCACACCCG AGGACCCGAA
    CAGCAAGTGA AAGCCATCCT GAGTGAGCTC TTACAGAGGG AGAACCGTGT CCTGCACTTC
    TGGACATTGA AGAAGCGGCG GTTAGACCAG TGTCAGCAAT ACGTGGTATT TGAGCGAAGC
    GCCAAGCAGG CACTAGACTG GATCCAAGAA ACAGGTGAAT ATTACCTCTC AACACATACC
    TCCACCGGGG AGACCACAGA GGAGACTCAG GAACTGCTGA AAGAATATGG GGAGTTCAGG
    GTGCCTGCCA AGCAAACAAA GGAGAAGGTG AAGCTTCTGA TTCAGCTGGC TGATAGCTTT
    GTGGAAAAAG GTCACATTCA TGCCACAGAG ATCAGGAAAT GGGTGACCAC AGTGGACAAG
    CATTACAGAG ACTTCTCACT GAGGATGGGA AAATACCGGT ACTCCCTGGA GAAGGCCCTA
    GGAGTCAACA CGGAGGATAA CAAGGACCTG GAGCTGGACA TCATCCCAGC AAGCCTTTCA
    GATCGTGAGG TCAAGCTCCG GGATGCCAAC CACGAGGTCA ATGAAGAGAA GCGGAAATCA
    GCCAGGAAAA AAGAATTCAT TATGGCAGAA TTGCTCCAGA CAGAGAAGGC TTATGTGAGG
    GACTTACATG AGTGCTTAGA GACCTACCTG TGGGAAATGA CCAGTGGTGT GGAGGAGATC
    CCCCCTGGGA TCCTTAATAA GGAGCATATC ATCTTTGGCA ACATCCAAGA GATCTACGAT
    TTTCATAACA ACATCTTCCT CAAAGAACTG GAGAAGTATG AGCAGCTGCC TGAGGATGTG
    GGACACTGCT TTGTTACTTG GGCAGACAAA TTTCAGATGT ACGTCACCTA CTGTAAAAAC
    AAGCCTGATT CCAACCAGCT GATCCTGGAG CATGCAGGCA CCTTCTTTGA TGAGATACAG
    CAGCGGCATG GTCTGGCCAA CTCCATTTCT TCCTACCTAA TTAAGCCTGT CCAGAGGATT
    ACCAAGTACC AGCTGCTCCT GAAGGAACTT TTAACTTGCT GTGAAGAAGG AAAAGGGGAG
    CTCAAGGAAG GCCTGGAGGT GATGCTTAGT GTCCCAAAGA GAGCCAACGA TGCCATGCAC
    GTCAGCATGC TGGAAGGGTT CGACGAGAAC CTGGACGTGC AGGGGGAGCT GATTTTACAA
    GATGCCTTTC AAGTGTGGGA CCCCAAGTCG CTGATCCGGA AAGGGCGGGA GCGACACCTA
    TTCCTCTTTG AGATCTCCTT GGTTTTCAGC AAGGAGATCA AAGACTCTTC AGGACACACA
    AAATATGTTT ACAAGAACAA GCTACTGACC TCAGAGCTGG GTGTGACCGA ACACGTAGAG
    GGCGATCCCT GCAAATTCGC CTTGTGGTCT GGGCGCACCC CATCCTCAGA CAATAAAACA
    GTGCTGAAAG CCTCCAACAT AGAAACCAAG CAGGAGTGGA TCAAGAACAT TCGAGAGGTG
    ATTCAAGAAA GGATCATTCA CCTGAAAGGA GCTTTGAAGG AGCCAATTCA GCTCCCCAAA
    ACACCAGCCA AACAGAGGAA CAACAGTAAG AGGGATGGAG TGGAGGATGT TGACAGCCAG
    GGGGATGGGA GCAGCCAGCC AGACACCATC TCCATTGCCT CTAGGACCTC CCAGAACACA
    GCGGACAGTG ACAAGCTCTC TGGCGGCTGT GAGTTGACCG TGGTGCTCCA GGACTTCAAC
    GCAGGCCACA GCAGCGAGCT GAGCATCCAG GTGGGACAGA CGGTGGAGCT GCTAGAGCGG
    CCGAGCGAGC GGCCGGGCTG GTGTCTGGTC CGAACCACAG AGCGGAGCCC CCCCCAGGAG
    GGCCTGGTGC CCAGCAGCGC CCTGTGCATC TCCCACTCCC GAAGCAGCGT GGAGATGGAC
    TGCTTCTTCC CCCTGGTGAA AGATGCGTAC TCTCATTCCT CAAGTGAAAA CGGAGGCAAG
    TCCGAGTCGG TGGCCAACCT GCAGGCCCAA CCATCCCTGA ACTCCATCCA CAGCTCCCCT
    GGGCCCAAGC GTTCCACCAA CACCCTGAAG AAGTGGCTGA CGAGTCCCGT GCGCCGGCTT
    AACAGTGGGA AAGCAGATGG AAACATCAAG AAGCAGAAGA AAGTACGAGA TGGTCGGAAG
    AGCTTTGACC TGGGATCTCC CAAGCCTGGG GATGAGACGA CCCCTCAGGG AGACAGCGCT
    GATGAGAGCA AGAAAGGTTG GGGTGAAGAT GAGGCAGATG AAGAGTCACA CACACCCCTC
    CCCCCGCCTA TGAAGATCTT TGACAACGAC CCCACGCAGG ACGAAATGAG TCTGGAAGGA
    GGCTCTTACC GAGGGAGCTT GAAAGACCCC ACAGGCTGCC TGAATGAAGG CATGACCCCA
    CCCACACCTC CCAGAAATCT GGAAGAAGAA CAGAAAGCCA AGGCCCTGAG AGGCAGGATG
    TTTGTCCTGA ATGAACTGGT ACAAACAGAG AAGGACTATG TCAAGGACCT GGGAATTGTG
    GTGGAGGGTT TCATGAAGAG AATAGAAGAA AAGGGTGTCC CCGAGGACAT GCGAGGAAAG
    GATAAAATCG TGTTTGGAAA TATTCACCAG ATTTATGATT GGCATAAGGA TGTTTTCCTG
    GGTGAACTGG AAAAATGTAT CCAGGAGCAA GACAGATTGG CACAGCTCTT TATTAAATAT
    GAGCGGAAGC TGCACATCTA TGTGTGGTAC TGCCAGAACA AGCCGCGCTC CGAGTACATC
    GTCGCCGAGT ACGACGCCTA CTTTGAAGAG GTGAAACAAG AGATAAATCA AAGGCTGACG
    CTTAGCGACT TCCTTATCAA GCCCATTCAG AGAATAACAA AATATCAATT GCTCCTCAAG
    GACTTCCTGA GATACAGCGA GAAGGCTGGT TTGGAGTGTT CAGATATTGA GAAAGCAGTG
    GAGTTAATGT GCCTTGTTCC AAAACGCTGC AATGACATGA TGAACCTGGG GCGCCTGCAG
    GGCTTTGAGG GGACCCTGAC TGCTCAGGGC AAGCTGCTGC AGCAGGACAC ATTCTACGTG
    ATTGAGCTGG ACGCTGGCAT GCAGTCGCGG ACCAAGGAGA GGCGTGTGTT TCTCTTTGAG
    CAAATCGTCA TCTTCAGTGA ACTGCTCAGG AAGGGATCCC TCACCCCAGG CTACATGTTC
    AAAAGGAGCA TCAAGATGAA CTACTTGGTC CTGGAGGAGA ACGTGGACAA TGACCCCTGC
    AAGTTTGCGC TTCTGAACAG GGAGACTTCA GAGAGGGTTA TTCTGCAAGC CGCCAATGCT
    GACATCCAGC AGGCCTGGGT GCAGGACATC AACCAAGTCT TAGAAACACA GCGAGACTTC
    TTAAATGCCC TACAGTCGCC CATTGAGTAT CAGCGGAAAG AAAGGAGCAC AGCCGTGATG
    AGGTCCCAGC CTGCTAGGGT TCCCCAAGCC AGCCCCAGGC CCTACTCCTC CATCCCCGTG
    GGCTCCGAGA AGCCCCCAAA GGGCTCCAGC TATAACCCGC CTCTGCCACC CCTGAAGATA
    TCTACCTCCA ATGGCAGTCC AGGGTTTGAC TACCACCAGG CTGGGGACAA GTTTGAGGCC
    AGCAAGAATG ACCTGGGAGG CTGCAACGGG GCCTCATCCA TGGCCGTGAT CAAAGATTAT
    TATGCACTGA AGGAGAACGA AATCTGTGTG AGCCAAGGTG AGGTGGTCCA GGTCCTCGCC
    GTCAACCAGC AGAACATGTG CCTGGTGTAC CAGCCCGCTA GCGACCACTC CCCCGCCGCT
    GAGGGCTGGG TCCCTGGCAG CATCCTGACG CCCCTCACCA AAGCCATGGC AGCCACAGAA
    AATAGTGACG GGAGCATCAA GAAGTCATGT TCATGGCATA CTCTACGCAT GAGAAAGCGG
    GCAGAAGTGG AGAACACGGG TAAAAATGAA GCCACAGGGC CTCGTAAACC CAAGGATATT
    CTGGGCAACA AAGTCTCTGT TAAAGAGACG AACAGTTCCG AGGAGTCAGA GTGTGATGAT
    CTTGACCCTA ATACTAGCAT GGAGATCTTA AATCCAAATT TCATCCAAGA AGTGGCGCCA
    GAATTCCTCG TGCCCTTAGT GGATGTGACC TGCTTGCTTG GGGACACAGT GACTCTGCAG
    TGCAAAGTCT GTGGGCGGCC AAAGCCCACC ATCACTTGGA AGGGTCCAGA CCAGAACATC
    CTTGACACTG ACAACAGCTC AGCTACATAC ACCGTCTCCT CCTGTGACTC TGGGGAAATC
    ACCCTGAAAA TCTGCAATCT GATGCCCCAA GACAGCGGTA TCTATACCTG CATAGCAGCA
    AACGACCATG GTACTGCGTC AACATCTGCT ACTGTTAAAG TGCAAGGTGT TCCAGCGGCC
    CCTAACCGCC CCATTGCCCA AGAGAGAAGC TGCACTTCCG TGATCCTCCG CTGGCTGCCC
    CCATCCAGCA CGGGAAACTG CACCATTTCC GGTTACACCG TGGAGTACAG AGAGGAAGGT
    TCCCAGGCCT GGCAGCAGTC AGTAGCATCG ACCTTGGACA CCTACCTTGT CATTGAAGAC
    CTCAGTCCTG GGAGTCCTTA TCAGTTCAGA GTCAGTGCCA GTAACCCCTG GGGGATCAGC
    CTGCCCAGCG AGCCCTCAGA ATTCGTGCGA CTTCCAGATT ACGACGCTGC TGCCGATGGT
    GCCACCATTT CTTGGAAGGA AAATTTTGAT TCAGCTTATA CTGAGCTGAA TGAAATTGGA
    AGAGGCCGGT TCTCCATAGT AAAGAAGTGC ATTCACAAAG CTACTCGCAA AGATGTCGCT
    GTGAAATTTG TTAGCAAAAA AATGAAGAAA AAAGAGCAGG CTGCCCACGA GGCTGCCCTG
    CTTCAGCACC TGCAGCACCC CCAATACATC ACTCTCCATG ACACCTATGA GTCCCCCACT
    TCCTACATCC TCATTTTGGA ACTGATGGAC GATGGCCGAC TCTTAGACTA TCTTATGAAT
    CATGATGAGC TGATGGAAGA AAAAGTAGCT TTCTACATTC GAGACATCAT GGAAGCCCTG
    CAGTACCTTC ATAACTGCAG AGTTGCACAT TTGGACATAA AGCCTGAAAA CCTGCTCATT
    GACCTACGGA TTCCAGTGCC TCGAGTGAAG CTCATTGACT TGGAGGATGC TGTCCAGATC
    TCAGGTCACT TCCATATCCA CCACCTTCTG GGGAACCCCG AGTTTGCTGC CCCAGAAGTG
    ATCCAGGGCA TCCCTGTATC CCTGGGCACC GACATCTGGA GCATCGGGGT TTTGACATAC
    GTCATGCTGA GTGGGGTCTC CCCCTTCTTG GATGAGAGCA AGGAGGAGAC ATGTATCAAT
    GTGTGCAGGG TGGACTTCAG CTTCCCCCAT GAGTACTTCT GCGGTGTGAG CAATGCTGCC
    AGAGATTTCA TCAATGTGAT CCTACAGGAA GACTTTCGGA GGCGGCCCAC AGCAGCCACC
    TGCCTGCAGC ATCCGTGGCT GCAGCCCCAC AATGGCAGCT ACTCCAAGAT CCCCCTGGAC
    ACCTCCCGCT TGGCGTGCTT CATCGAGCGC CGCAAGCATC AGAATGATGT GCGCCCCATT
    CCCAACGTCA AGAGCTACAT TGTCAATCGG CTAAACCAAG GCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.