SBNO1 cDNA ORF clone, Bos taurus(cattle)

The following SBNO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SBNO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa08999 XM_002694566.6
Latest version!
Bos taurus strawberry notch homolog 1 (SBNO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa08999 XM_865383.8
Latest version!
Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa09000 XM_005195024.3
Latest version!
Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa09000 XM_005217972.4
Latest version!
Bos taurus strawberry notch homolog 1 (SBNO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54721 XM_010823358.2
Latest version!
Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54721 XM_010814004.3
Latest version!
Bos taurus strawberry notch homolog 1 (SBNO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54722 XM_010823359.2
Latest version!
Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54722 XM_010814005.3
Latest version!
Bos taurus strawberry notch homolog 1 (SBNO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa54722 XM_010814005.2 Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa08999 XM_002694566.5 Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa09000 XM_005217972.3 Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa54721 XM_010814004.2 Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa08999
    Clone ID Related Accession (Same CDS sequence) XM_002694566.5 , XM_865383.8 , XM_002694566.6
    Accession Version XM_002694566.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4182bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein strawberry notch homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002694566.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGTCAGTGCC ACTTAGTGCA TTAGAACTAG GTTTGGAGAC TGAAGCAGCA
    GTTCCTGTTA AACAAGAACC AGAGACGGTA CCTACTCCAG CACTTTTAAA TGTTAGGCAG
    CAGCCTCCAT CTACTACAAC ATTTGTGCTG AATCAAATAA ATCAGCTTCC AACCTTGGGA
    TCTACAATTG TAATGACTAA GACACCACCT GTAACCACGA ATAGGCAAAC CATCACTTTA
    ACGAAGTTCA TTCAGACTAC TGCAAACACA CGCCCTTCAG TCTCAGCACC AGCAGTACGA
    AATGCCATGA CTTCCGCACC TTCAAAAGAC CAAGTTCAAC TGAAGGACCT GCTAAAAAAT
    AATAGTCTGA ATGAACTCAT GAAACTGAAG CCACCTGCTA ATATTGCTCA GCCAGTTGCA
    ACAGCAGCTA CTGATGTAAG CAATGGCACA GTAAAGAAAG AGTCTTCTAA TAAAGAAGTA
    GCAAGAATAT GGATAAATGA CATGAAAATG AGGAGTTTTT CCCCAACCAT GAAGGTCCCT
    GTTGTGAAAG AGGAAGATGA ACCAGAAGAA GAAGACGAAG AAGAAATGGG TCATGCGGAA
    ACCTATGCAG AGTATATGCC AATAAAATTA AAAATTGGCC TACGTCACCC AGATGCCGTA
    GTTGAGACTA GTTCTTTATC CAGTGTTACT CCTCCTGATG TTTGGTACAA GACATCTATT
    TCTGAAGAAA CCATTGATAA TGGCTGGTTA TCAGCATTAC AGCTTGAAGC AATTACTTAT
    GCAGCCCAGC AACATGAAAC GTTCCTACCT AATGGAGATC GTGCTGGCTT CTTAATAGGC
    GATGGTGCTG GTGTAGGAAA AGGAAGGACA ATAGCAGGAA TCATCTATGA AAATTATTTG
    TTGAGTAGAA AAAGGGCTTT GTGGTTCAGT GTTTCAAATG ATTTAAAGTA TGATGCTGAA
    AGAGATTTGA GGGATATTGG AGCAAAAAAC ATTTTGGTCC ATTCATTGAA TAAGTTTAAA
    TATGGAAAAA TTTCTTCCAA ACATAATGGG AGTGTGAAGA AGGGTGTTAT TTTTGCTACC
    TATTCTTCCC TTATTGGTGA AAGTCAGTCT GGTGGTAAAT ATAAAACGAG GTTAAAACAA
    CTTCTACATT GGTGCGGTGA TGACTTCGAT GGAGTGATAG TTTTTGATGA ATGTCATAAA
    GCAAAAAACT TATGTCCTGT TGGTTCTTCA AAGCCAACCA AGACAGGCTT AGCAGTTTTA
    GAACTTCAGA ACAAATTGCC AAAAGCTAGA GTTGTCTATG CTAGTGCCAC TGGTGCTTCT
    GAACCACGAA ATATGGCCTA TATGAACCGT CTTGGAATAT GGGGTGAGGG AACTCCATTT
    AGAGAATTCA GTGACTTTAT TCAGGCAGTA GAACGGAGAG GTGTTGGTGC CATGGAAATA
    GTTGCTATGG ATATGAAGCT TAGAGGAATG TACATTGCTC GACAGCTGAG CTTTACTGGA
    GTGACCTTCA AAATTGAGGA AGTTCTTCTT TCTCAGAGCT ATGTTAAAAT GTATAACAAA
    GCAGTCAAAC TGTGGGTCAT TGCTCGAGAG CGGTTTCAGC AAGCTGCAGA TCTGATTGAT
    GCTGAGCAGA GAATGAAGAA ATCCATGTGG GGTCAGTTCT GGTCTGCTCA CCAGAGATTT
    TTCAAATACT TGTGCATAGC GTCCAAAGTT AAAAGAGTTG TGCAACTAGC TCGAGAAGAA
    ATCAAGAATG GAAAATGTGT TGTAATTGGT CTGCAGTCTA CGGGAGAAGC TAGAACATTA
    GAAGCCTTGG AAGAGGGCGG GGGAGAATTG AATGATTTTG TTTCAACTGC CAAAGGAGTG
    TTACAGTCAC TCATTGAAAA ACACTTCCCT GCTCCGGATA GAAAAAAGCT TTATAGTTTG
    CTGGGAATTG ATTTGACAGC TCCAAGTAAC AGCAGTTCAC CAAGAGATAG TCCTTGTAAG
    GAAAATAAAA CAAAGAAGCG GAAAGGTGAA GAAATAACTC GAGAAGCCAA AAAAGCACGA
    AAAATAGGTG GCCTTACTGG TAGCAGTTCT GATGATAGTG GAAGTGAATC TGATGCCTCT
    GATAATGAAG AAAGTGACTA TGAGAGCTCT AAAAACATGA GTTCTGGAGA TGATGATGAT
    TTCAACCCAT TCAGAGATGA GTCTAGTGAG GATGATGAAG ATGATCCCTG GTTAATCAGA
    AAAGATCATA AGAAAAACAA AGATAAAAAA AAAAGGAAAA GTATAGATCC AGATTCTATT
    CAAAGTGCCT TATTAGCGTC AGGTCTTGGA TCAAAACGAC CTAGTTTTTC TTCTACACCA
    GTTATTTCAC CTGCTCCTAA CAGTGCACCA GCTAACAGTA ACACCAACAG CAACAGTAGC
    CTTATAACAA GTCAAGATGC CGTGGAAAGG GCCCAACAGA TGAAGAAAGA CCTACTTGAT
    AAACTAGAAA AATTAGCTGA AGACCTCCCT CCTAATACCC TGGATGAACT TATTGATGAA
    CTTGGTGGCC CTGAAAATGT TGCTGAGATG ACTGGCCGCA AGGGGAGGGT CGTAAGCAAT
    GATGACGGAA GCATATCTTA TGAGTCAAGA TCTGAACTTG ATGTTCCTGT GGAAATACTA
    AATATTACAG AAAAACAAAG ATTTATGGAT GGAGATAAGA ATATTGCTAT CATCTCAGAA
    GCTGCCAGCT CAGGTATTTC ATTACAAGCG GATAGAAGGG CTAAAAATCA AAGGCGAAGA
    GTTCATATGA CTCTGGAGTT ACCATGGAGT GCTGATAGAG CAATTCAGCA ATTTGGACGA
    ACTCATAGAT CAAATCAAGT TACCGCTCCT GAATATGTCT TTCTGATTTC TGAATTGGCA
    GGAGAACAAA GATTTGCATC TATTGTTGCT AAAAGACTTG AGAGTTTGGG CGCACTTACA
    CATGGAGACA GAAGAGCAAC AGAATCTAGA GATCTGAGCC GGTTCAACTT CGATAATAAG
    TATGGAAGAA ATGCTTTAGA AATTGTCATG AAATCCATTG TAAACCTAGA TTCTCCTATG
    GTATCACCAC CTCCAGACTA TCCTGGAGAA TTCTTTAAAG ATGTTCGACA AGGACTGATA
    GGAGTTGGTC TAATAAATGT AGAAGATCGT TCAGGAATTC TTACTCTAGA TAAAGATTAT
    AACAACATAG GAAAATTCTT AAACAGAATT TTGGGCATGG AAGTGCATCA ACAGAATGCA
    CTATTTCAGT ATTTCGCAGA CACACTTACT GCAGTCGTTC AAAATGCCAA AAAAAATGGA
    AGATACGATA TGGGAATCTT AGATCTTGGT TCTGGGGATG AAAAAGTGAG GAAAAGTGAT
    GTTAAGAAGT TTCTGACTCC AGGATATTCA ACATCTGGCC ATGTAGAATT ATACACAATA
    AGTGTGGAGA GGGGAATGTC CTGGGAGGAA GCTACCAAGA TTTGGGCTGA GCTGACAGGA
    CCAGATGATG GCTTTTATTT GTCATTGCAA ATAAGAAACA ACAAGAAAAC TGCCATCTTA
    GTTAAAGAAG TAAACCCTAA AAAGAAGCTT TTCTTAGTTT ATAGACCAAA TACCGGGAAA
    CAACTCAAAT TAGAAATTTA TGCTGATCTA AAAAAGAAAT ATAAGAAGGT AGTCTCAGAC
    GATGCCGTGG TCCACTGGTT AGATCAGTAT AATTCATCTG CAGATACTTG TACTCATGCT
    TATTGGCGTG GCAACTGCAA AAAAGCAAGC TTGGGATTAG TTTGTGAAAT AGGTCTTCGT
    TGTCGCACAT ACTATGTATT ATGTGGCTCA GTACTGAGTG TCTGGACAAA AGTTGAGGGT
    GTTCTTGCGT CTGTCAGTGG CACAAATGTG AAAATGCAGA TAGTTCGCCT AAGAACAGAG
    GATGGACAGC GGATTGTAGG TTTGATCATT CCTGCAAATT GTGTGTCTCC TCTTATAAAT
    CTCCTTTCAA CTTCAGACCA GTCTCAACAG CTTGCAGTCC AGCAGAAACA GCTCTGGCAG
    CAGCGTCACC CCCAGAGCAT TGCCAGCTTG AGCAGCACAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694612.2
    CDS136..4317
    Translation

    Target ORF information:

    RefSeq Version XM_002694566.5
    Organism Bos taurus(cattle)
    Definition Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694566.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGTCAGTGCC ACTTAGTGCA TTAGAACTAG GTTTGGAGAC TGAAGCAGCA
    GTTCCTGTTA AACAAGAACC AGAGACGGTA CCTACTCCAG CACTTTTAAA TGTTAGGCAG
    CAGCCTCCAT CTACTACAAC ATTTGTGCTG AATCAAATAA ATCAGCTTCC AACCTTGGGA
    TCTACAATTG TAATGACTAA GACACCACCT GTAACCACGA ATAGGCAAAC CATCACTTTA
    ACGAAGTTCA TTCAGACTAC TGCAAACACA CGCCCTTCAG TCTCAGCACC AGCAGTACGA
    AATGCCATGA CTTCCGCACC TTCAAAAGAC CAAGTTCAAC TGAAGGACCT GCTAAAAAAT
    AATAGTCTGA ATGAACTCAT GAAACTGAAG CCACCTGCTA ATATTGCTCA GCCAGTTGCA
    ACAGCAGCTA CTGATGTAAG CAATGGCACA GTAAAGAAAG AGTCTTCTAA TAAAGAAGTA
    GCAAGAATAT GGATAAATGA CATGAAAATG AGGAGTTTTT CCCCAACCAT GAAGGTCCCT
    GTTGTGAAAG AGGAAGATGA ACCAGAAGAA GAAGACGAAG AAGAAATGGG TCATGCGGAA
    ACCTATGCAG AGTATATGCC AATAAAATTA AAAATTGGCC TACGTCACCC AGATGCCGTA
    GTTGAGACTA GTTCTTTATC CAGTGTTACT CCTCCTGATG TTTGGTACAA GACATCTATT
    TCTGAAGAAA CCATTGATAA TGGCTGGTTA TCAGCATTAC AGCTTGAAGC AATTACTTAT
    GCAGCCCAGC AACATGAAAC GTTCCTACCT AATGGAGATC GTGCTGGCTT CTTAATAGGC
    GATGGTGCTG GTGTAGGAAA AGGAAGGACA ATAGCAGGAA TCATCTATGA AAATTATTTG
    TTGAGTAGAA AAAGGGCTTT GTGGTTCAGT GTTTCAAATG ATTTAAAGTA TGATGCTGAA
    AGAGATTTGA GGGATATTGG AGCAAAAAAC ATTTTGGTCC ATTCATTGAA TAAGTTTAAA
    TATGGAAAAA TTTCTTCCAA ACATAATGGG AGTGTGAAGA AGGGTGTTAT TTTTGCTACC
    TATTCTTCCC TTATTGGTGA AAGTCAGTCT GGTGGTAAAT ATAAAACGAG GTTAAAACAA
    CTTCTACATT GGTGCGGTGA TGACTTCGAT GGAGTGATAG TTTTTGATGA ATGTCATAAA
    GCAAAAAACT TATGTCCTGT TGGTTCTTCA AAGCCAACCA AGACAGGCTT AGCAGTTTTA
    GAACTTCAGA ACAAATTGCC AAAAGCTAGA GTTGTCTATG CTAGTGCCAC TGGTGCTTCT
    GAACCACGAA ATATGGCCTA TATGAACCGT CTTGGAATAT GGGGTGAGGG AACTCCATTT
    AGAGAATTCA GTGACTTTAT TCAGGCAGTA GAACGGAGAG GTGTTGGTGC CATGGAAATA
    GTTGCTATGG ATATGAAGCT TAGAGGAATG TACATTGCTC GACAGCTGAG CTTTACTGGA
    GTGACCTTCA AAATTGAGGA AGTTCTTCTT TCTCAGAGCT ATGTTAAAAT GTATAACAAA
    GCAGTCAAAC TGTGGGTCAT TGCTCGAGAG CGGTTTCAGC AAGCTGCAGA TCTGATTGAT
    GCTGAGCAGA GAATGAAGAA ATCCATGTGG GGTCAGTTCT GGTCTGCTCA CCAGAGATTT
    TTCAAATACT TGTGCATAGC GTCCAAAGTT AAAAGAGTTG TGCAACTAGC TCGAGAAGAA
    ATCAAGAATG GAAAATGTGT TGTAATTGGT CTGCAGTCTA CGGGAGAAGC TAGAACATTA
    GAAGCCTTGG AAGAGGGCGG GGGAGAATTG AATGATTTTG TTTCAACTGC CAAAGGAGTG
    TTACAGTCAC TCATTGAAAA ACACTTCCCT GCTCCGGATA GAAAAAAGCT TTATAGTTTG
    CTGGGAATTG ATTTGACAGC TCCAAGTAAC AGCAGTTCAC CAAGAGATAG TCCTTGTAAG
    GAAAATAAAA CAAAGAAGCG GAAAGGTGAA GAAATAACTC GAGAAGCCAA AAAAGCACGA
    AAAATAGGTG GCCTTACTGG TAGCAGTTCT GATGATAGTG GAAGTGAATC TGATGCCTCT
    GATAATGAAG AAAGTGACTA TGAGAGCTCT AAAAACATGA GTTCTGGAGA TGATGATGAT
    TTCAACCCAT TCAGAGATGA GTCTAGTGAG GATGATGAAG ATGATCCCTG GTTAATCAGA
    AAAGATCATA AGAAAAACAA AGATAAAAAA AAAAGGAAAA GTATAGATCC AGATTCTATT
    CAAAGTGCCT TATTAGCGTC AGGTCTTGGA TCAAAACGAC CTAGTTTTTC TTCTACACCA
    GTTATTTCAC CTGCTCCTAA CAGTGCACCA GCTAACAGTA ACACCAACAG CAACAGTAGC
    CTTATAACAA GTCAAGATGC CGTGGAAAGG GCCCAACAGA TGAAGAAAGA CCTACTTGAT
    AAACTAGAAA AATTAGCTGA AGACCTCCCT CCTAATACCC TGGATGAACT TATTGATGAA
    CTTGGTGGCC CTGAAAATGT TGCTGAGATG ACTGGCCGCA AGGGGAGGGT CGTAAGCAAT
    GATGACGGAA GCATATCTTA TGAGTCAAGA TCTGAACTTG ATGTTCCTGT GGAAATACTA
    AATATTACAG AAAAACAAAG ATTTATGGAT GGAGATAAGA ATATTGCTAT CATCTCAGAA
    GCTGCCAGCT CAGGTATTTC ATTACAAGCG GATAGAAGGG CTAAAAATCA AAGGCGAAGA
    GTTCATATGA CTCTGGAGTT ACCATGGAGT GCTGATAGAG CAATTCAGCA ATTTGGACGA
    ACTCATAGAT CAAATCAAGT TACCGCTCCT GAATATGTCT TTCTGATTTC TGAATTGGCA
    GGAGAACAAA GATTTGCATC TATTGTTGCT AAAAGACTTG AGAGTTTGGG CGCACTTACA
    CATGGAGACA GAAGAGCAAC AGAATCTAGA GATCTGAGCC GGTTCAACTT CGATAATAAG
    TATGGAAGAA ATGCTTTAGA AATTGTCATG AAATCCATTG TAAACCTAGA TTCTCCTATG
    GTATCACCAC CTCCAGACTA TCCTGGAGAA TTCTTTAAAG ATGTTCGACA AGGACTGATA
    GGAGTTGGTC TAATAAATGT AGAAGATCGT TCAGGAATTC TTACTCTAGA TAAAGATTAT
    AACAACATAG GAAAATTCTT AAACAGAATT TTGGGCATGG AAGTGCATCA ACAGAATGCA
    CTATTTCAGT ATTTCGCAGA CACACTTACT GCAGTCGTTC AAAATGCCAA AAAAAATGGA
    AGATACGATA TGGGAATCTT AGATCTTGGT TCTGGGGATG AAAAAGTGAG GAAAAGTGAT
    GTTAAGAAGT TTCTGACTCC AGGATATTCA ACATCTGGCC ATGTAGAATT ATACACAATA
    AGTGTGGAGA GGGGAATGTC CTGGGAGGAA GCTACCAAGA TTTGGGCTGA GCTGACAGGA
    CCAGATGATG GCTTTTATTT GTCATTGCAA ATAAGAAACA ACAAGAAAAC TGCCATCTTA
    GTTAAAGAAG TAAACCCTAA AAAGAAGCTT TTCTTAGTTT ATAGACCAAA TACCGGGAAA
    CAACTCAAAT TAGAAATTTA TGCTGATCTA AAAAAGAAAT ATAAGAAGGT AGTCTCAGAC
    GATGCCGTGG TCCACTGGTT AGATCAGTAT AATTCATCTG CAGATACTTG TACTCATGCT
    TATTGGCGTG GCAACTGCAA AAAAGCAAGC TTGGGATTAG TTTGTGAAAT AGGTCTTCGT
    TGTCGCACAT ACTATGTATT ATGTGGCTCA GTACTGAGTG TCTGGACAAA AGTTGAGGGT
    GTTCTTGCGT CTGTCAGTGG CACAAATGTG AAAATGCAGA TAGTTCGCCT AAGAACAGAG
    GATGGACAGC GGATTGTAGG TTTGATCATT CCTGCAAATT GTGTGTCTCC TCTTATAAAT
    CTCCTTTCAA CTTCAGACCA GTCTCAACAG CTTGCAGTCC AGCAGAAACA GCTCTGGCAG
    CAGCGTCACC CCCAGAGCAT TGCCAGCTTG AGCAGCACAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa08999
    Clone ID Related Accession (Same CDS sequence) XM_002694566.5 , XM_865383.8 , XM_002694566.6
    Accession Version XM_865383.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4182bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein strawberry notch homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_865383.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGTCAGTGCC ACTTAGTGCA TTAGAACTAG GTTTGGAGAC TGAAGCAGCA
    GTTCCTGTTA AACAAGAACC AGAGACGGTA CCTACTCCAG CACTTTTAAA TGTTAGGCAG
    CAGCCTCCAT CTACTACAAC ATTTGTGCTG AATCAAATAA ATCAGCTTCC AACCTTGGGA
    TCTACAATTG TAATGACTAA GACACCACCT GTAACCACGA ATAGGCAAAC CATCACTTTA
    ACGAAGTTCA TTCAGACTAC TGCAAACACA CGCCCTTCAG TCTCAGCACC AGCAGTACGA
    AATGCCATGA CTTCCGCACC TTCAAAAGAC CAAGTTCAAC TGAAGGACCT GCTAAAAAAT
    AATAGTCTGA ATGAACTCAT GAAACTGAAG CCACCTGCTA ATATTGCTCA GCCAGTTGCA
    ACAGCAGCTA CTGATGTAAG CAATGGCACA GTAAAGAAAG AGTCTTCTAA TAAAGAAGTA
    GCAAGAATAT GGATAAATGA CATGAAAATG AGGAGTTTTT CCCCAACCAT GAAGGTCCCT
    GTTGTGAAAG AGGAAGATGA ACCAGAAGAA GAAGACGAAG AAGAAATGGG TCATGCGGAA
    ACCTATGCAG AGTATATGCC AATAAAATTA AAAATTGGCC TACGTCACCC AGATGCCGTA
    GTTGAGACTA GTTCTTTATC CAGTGTTACT CCTCCTGATG TTTGGTACAA GACATCTATT
    TCTGAAGAAA CCATTGATAA TGGCTGGTTA TCAGCATTAC AGCTTGAAGC AATTACTTAT
    GCAGCCCAGC AACATGAAAC GTTCCTACCT AATGGAGATC GTGCTGGCTT CTTAATAGGC
    GATGGTGCTG GTGTAGGAAA AGGAAGGACA ATAGCAGGAA TCATCTATGA AAATTATTTG
    TTGAGTAGAA AAAGGGCTTT GTGGTTCAGT GTTTCAAATG ATTTAAAGTA TGATGCTGAA
    AGAGATTTGA GGGATATTGG AGCAAAAAAC ATTTTGGTCC ATTCATTGAA TAAGTTTAAA
    TATGGAAAAA TTTCTTCCAA ACATAATGGG AGTGTGAAGA AGGGTGTTAT TTTTGCTACC
    TATTCTTCCC TTATTGGTGA AAGTCAGTCT GGTGGTAAAT ATAAAACGAG GTTAAAACAA
    CTTCTACATT GGTGCGGTGA TGACTTCGAT GGAGTGATAG TTTTTGATGA ATGTCATAAA
    GCAAAAAACT TATGTCCTGT TGGTTCTTCA AAGCCAACCA AGACAGGCTT AGCAGTTTTA
    GAACTTCAGA ACAAATTGCC AAAAGCTAGA GTTGTCTATG CTAGTGCCAC TGGTGCTTCT
    GAACCACGAA ATATGGCCTA TATGAACCGT CTTGGAATAT GGGGTGAGGG AACTCCATTT
    AGAGAATTCA GTGACTTTAT TCAGGCAGTA GAACGGAGAG GTGTTGGTGC CATGGAAATA
    GTTGCTATGG ATATGAAGCT TAGAGGAATG TACATTGCTC GACAGCTGAG CTTTACTGGA
    GTGACCTTCA AAATTGAGGA AGTTCTTCTT TCTCAGAGCT ATGTTAAAAT GTATAACAAA
    GCAGTCAAAC TGTGGGTCAT TGCTCGAGAG CGGTTTCAGC AAGCTGCAGA TCTGATTGAT
    GCTGAGCAGA GAATGAAGAA ATCCATGTGG GGTCAGTTCT GGTCTGCTCA CCAGAGATTT
    TTCAAATACT TGTGCATAGC GTCCAAAGTT AAAAGAGTTG TGCAACTAGC TCGAGAAGAA
    ATCAAGAATG GAAAATGTGT TGTAATTGGT CTGCAGTCTA CGGGAGAAGC TAGAACATTA
    GAAGCCTTGG AAGAGGGCGG GGGAGAATTG AATGATTTTG TTTCAACTGC CAAAGGAGTG
    TTACAGTCAC TCATTGAAAA ACACTTCCCT GCTCCGGATA GAAAAAAGCT TTATAGTTTG
    CTGGGAATTG ATTTGACAGC TCCAAGTAAC AGCAGTTCAC CAAGAGATAG TCCTTGTAAG
    GAAAATAAAA CAAAGAAGCG GAAAGGTGAA GAAATAACTC GAGAAGCCAA AAAAGCACGA
    AAAATAGGTG GCCTTACTGG TAGCAGTTCT GATGATAGTG GAAGTGAATC TGATGCCTCT
    GATAATGAAG AAAGTGACTA TGAGAGCTCT AAAAACATGA GTTCTGGAGA TGATGATGAT
    TTCAACCCAT TCAGAGATGA GTCTAGTGAG GATGATGAAG ATGATCCCTG GTTAATCAGA
    AAAGATCATA AGAAAAACAA AGATAAAAAA AAAAGGAAAA GTATAGATCC AGATTCTATT
    CAAAGTGCCT TATTAGCGTC AGGTCTTGGA TCAAAACGAC CTAGTTTTTC TTCTACACCA
    GTTATTTCAC CTGCTCCTAA CAGTGCACCA GCTAACAGTA ACACCAACAG CAACAGTAGC
    CTTATAACAA GTCAAGATGC CGTGGAAAGG GCCCAACAGA TGAAGAAAGA CCTACTTGAT
    AAACTAGAAA AATTAGCTGA AGACCTCCCT CCTAATACCC TGGATGAACT TATTGATGAA
    CTTGGTGGCC CTGAAAATGT TGCTGAGATG ACTGGCCGCA AGGGGAGGGT CGTAAGCAAT
    GATGACGGAA GCATATCTTA TGAGTCAAGA TCTGAACTTG ATGTTCCTGT GGAAATACTA
    AATATTACAG AAAAACAAAG ATTTATGGAT GGAGATAAGA ATATTGCTAT CATCTCAGAA
    GCTGCCAGCT CAGGTATTTC ATTACAAGCG GATAGAAGGG CTAAAAATCA AAGGCGAAGA
    GTTCATATGA CTCTGGAGTT ACCATGGAGT GCTGATAGAG CAATTCAGCA ATTTGGACGA
    ACTCATAGAT CAAATCAAGT TACCGCTCCT GAATATGTCT TTCTGATTTC TGAATTGGCA
    GGAGAACAAA GATTTGCATC TATTGTTGCT AAAAGACTTG AGAGTTTGGG CGCACTTACA
    CATGGAGACA GAAGAGCAAC AGAATCTAGA GATCTGAGCC GGTTCAACTT CGATAATAAG
    TATGGAAGAA ATGCTTTAGA AATTGTCATG AAATCCATTG TAAACCTAGA TTCTCCTATG
    GTATCACCAC CTCCAGACTA TCCTGGAGAA TTCTTTAAAG ATGTTCGACA AGGACTGATA
    GGAGTTGGTC TAATAAATGT AGAAGATCGT TCAGGAATTC TTACTCTAGA TAAAGATTAT
    AACAACATAG GAAAATTCTT AAACAGAATT TTGGGCATGG AAGTGCATCA ACAGAATGCA
    CTATTTCAGT ATTTCGCAGA CACACTTACT GCAGTCGTTC AAAATGCCAA AAAAAATGGA
    AGATACGATA TGGGAATCTT AGATCTTGGT TCTGGGGATG AAAAAGTGAG GAAAAGTGAT
    GTTAAGAAGT TTCTGACTCC AGGATATTCA ACATCTGGCC ATGTAGAATT ATACACAATA
    AGTGTGGAGA GGGGAATGTC CTGGGAGGAA GCTACCAAGA TTTGGGCTGA GCTGACAGGA
    CCAGATGATG GCTTTTATTT GTCATTGCAA ATAAGAAACA ACAAGAAAAC TGCCATCTTA
    GTTAAAGAAG TAAACCCTAA AAAGAAGCTT TTCTTAGTTT ATAGACCAAA TACCGGGAAA
    CAACTCAAAT TAGAAATTTA TGCTGATCTA AAAAAGAAAT ATAAGAAGGT AGTCTCAGAC
    GATGCCGTGG TCCACTGGTT AGATCAGTAT AATTCATCTG CAGATACTTG TACTCATGCT
    TATTGGCGTG GCAACTGCAA AAAAGCAAGC TTGGGATTAG TTTGTGAAAT AGGTCTTCGT
    TGTCGCACAT ACTATGTATT ATGTGGCTCA GTACTGAGTG TCTGGACAAA AGTTGAGGGT
    GTTCTTGCGT CTGTCAGTGG CACAAATGTG AAAATGCAGA TAGTTCGCCT AAGAACAGAG
    GATGGACAGC GGATTGTAGG TTTGATCATT CCTGCAAATT GTGTGTCTCC TCTTATAAAT
    CTCCTTTCAA CTTCAGACCA GTCTCAACAG CTTGCAGTCC AGCAGAAACA GCTCTGGCAG
    CAGCGTCACC CCCAGAGCAT TGCCAGCTTG AGCAGCACAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_870476.5
    CDS136..4317
    Translation

    Target ORF information:

    RefSeq Version XM_865383.8
    Organism Bos taurus(cattle)
    Definition Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_865383.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGTCAGTGCC ACTTAGTGCA TTAGAACTAG GTTTGGAGAC TGAAGCAGCA
    GTTCCTGTTA AACAAGAACC AGAGACGGTA CCTACTCCAG CACTTTTAAA TGTTAGGCAG
    CAGCCTCCAT CTACTACAAC ATTTGTGCTG AATCAAATAA ATCAGCTTCC AACCTTGGGA
    TCTACAATTG TAATGACTAA GACACCACCT GTAACCACGA ATAGGCAAAC CATCACTTTA
    ACGAAGTTCA TTCAGACTAC TGCAAACACA CGCCCTTCAG TCTCAGCACC AGCAGTACGA
    AATGCCATGA CTTCCGCACC TTCAAAAGAC CAAGTTCAAC TGAAGGACCT GCTAAAAAAT
    AATAGTCTGA ATGAACTCAT GAAACTGAAG CCACCTGCTA ATATTGCTCA GCCAGTTGCA
    ACAGCAGCTA CTGATGTAAG CAATGGCACA GTAAAGAAAG AGTCTTCTAA TAAAGAAGTA
    GCAAGAATAT GGATAAATGA CATGAAAATG AGGAGTTTTT CCCCAACCAT GAAGGTCCCT
    GTTGTGAAAG AGGAAGATGA ACCAGAAGAA GAAGACGAAG AAGAAATGGG TCATGCGGAA
    ACCTATGCAG AGTATATGCC AATAAAATTA AAAATTGGCC TACGTCACCC AGATGCCGTA
    GTTGAGACTA GTTCTTTATC CAGTGTTACT CCTCCTGATG TTTGGTACAA GACATCTATT
    TCTGAAGAAA CCATTGATAA TGGCTGGTTA TCAGCATTAC AGCTTGAAGC AATTACTTAT
    GCAGCCCAGC AACATGAAAC GTTCCTACCT AATGGAGATC GTGCTGGCTT CTTAATAGGC
    GATGGTGCTG GTGTAGGAAA AGGAAGGACA ATAGCAGGAA TCATCTATGA AAATTATTTG
    TTGAGTAGAA AAAGGGCTTT GTGGTTCAGT GTTTCAAATG ATTTAAAGTA TGATGCTGAA
    AGAGATTTGA GGGATATTGG AGCAAAAAAC ATTTTGGTCC ATTCATTGAA TAAGTTTAAA
    TATGGAAAAA TTTCTTCCAA ACATAATGGG AGTGTGAAGA AGGGTGTTAT TTTTGCTACC
    TATTCTTCCC TTATTGGTGA AAGTCAGTCT GGTGGTAAAT ATAAAACGAG GTTAAAACAA
    CTTCTACATT GGTGCGGTGA TGACTTCGAT GGAGTGATAG TTTTTGATGA ATGTCATAAA
    GCAAAAAACT TATGTCCTGT TGGTTCTTCA AAGCCAACCA AGACAGGCTT AGCAGTTTTA
    GAACTTCAGA ACAAATTGCC AAAAGCTAGA GTTGTCTATG CTAGTGCCAC TGGTGCTTCT
    GAACCACGAA ATATGGCCTA TATGAACCGT CTTGGAATAT GGGGTGAGGG AACTCCATTT
    AGAGAATTCA GTGACTTTAT TCAGGCAGTA GAACGGAGAG GTGTTGGTGC CATGGAAATA
    GTTGCTATGG ATATGAAGCT TAGAGGAATG TACATTGCTC GACAGCTGAG CTTTACTGGA
    GTGACCTTCA AAATTGAGGA AGTTCTTCTT TCTCAGAGCT ATGTTAAAAT GTATAACAAA
    GCAGTCAAAC TGTGGGTCAT TGCTCGAGAG CGGTTTCAGC AAGCTGCAGA TCTGATTGAT
    GCTGAGCAGA GAATGAAGAA ATCCATGTGG GGTCAGTTCT GGTCTGCTCA CCAGAGATTT
    TTCAAATACT TGTGCATAGC GTCCAAAGTT AAAAGAGTTG TGCAACTAGC TCGAGAAGAA
    ATCAAGAATG GAAAATGTGT TGTAATTGGT CTGCAGTCTA CGGGAGAAGC TAGAACATTA
    GAAGCCTTGG AAGAGGGCGG GGGAGAATTG AATGATTTTG TTTCAACTGC CAAAGGAGTG
    TTACAGTCAC TCATTGAAAA ACACTTCCCT GCTCCGGATA GAAAAAAGCT TTATAGTTTG
    CTGGGAATTG ATTTGACAGC TCCAAGTAAC AGCAGTTCAC CAAGAGATAG TCCTTGTAAG
    GAAAATAAAA CAAAGAAGCG GAAAGGTGAA GAAATAACTC GAGAAGCCAA AAAAGCACGA
    AAAATAGGTG GCCTTACTGG TAGCAGTTCT GATGATAGTG GAAGTGAATC TGATGCCTCT
    GATAATGAAG AAAGTGACTA TGAGAGCTCT AAAAACATGA GTTCTGGAGA TGATGATGAT
    TTCAACCCAT TCAGAGATGA GTCTAGTGAG GATGATGAAG ATGATCCCTG GTTAATCAGA
    AAAGATCATA AGAAAAACAA AGATAAAAAA AAAAGGAAAA GTATAGATCC AGATTCTATT
    CAAAGTGCCT TATTAGCGTC AGGTCTTGGA TCAAAACGAC CTAGTTTTTC TTCTACACCA
    GTTATTTCAC CTGCTCCTAA CAGTGCACCA GCTAACAGTA ACACCAACAG CAACAGTAGC
    CTTATAACAA GTCAAGATGC CGTGGAAAGG GCCCAACAGA TGAAGAAAGA CCTACTTGAT
    AAACTAGAAA AATTAGCTGA AGACCTCCCT CCTAATACCC TGGATGAACT TATTGATGAA
    CTTGGTGGCC CTGAAAATGT TGCTGAGATG ACTGGCCGCA AGGGGAGGGT CGTAAGCAAT
    GATGACGGAA GCATATCTTA TGAGTCAAGA TCTGAACTTG ATGTTCCTGT GGAAATACTA
    AATATTACAG AAAAACAAAG ATTTATGGAT GGAGATAAGA ATATTGCTAT CATCTCAGAA
    GCTGCCAGCT CAGGTATTTC ATTACAAGCG GATAGAAGGG CTAAAAATCA AAGGCGAAGA
    GTTCATATGA CTCTGGAGTT ACCATGGAGT GCTGATAGAG CAATTCAGCA ATTTGGACGA
    ACTCATAGAT CAAATCAAGT TACCGCTCCT GAATATGTCT TTCTGATTTC TGAATTGGCA
    GGAGAACAAA GATTTGCATC TATTGTTGCT AAAAGACTTG AGAGTTTGGG CGCACTTACA
    CATGGAGACA GAAGAGCAAC AGAATCTAGA GATCTGAGCC GGTTCAACTT CGATAATAAG
    TATGGAAGAA ATGCTTTAGA AATTGTCATG AAATCCATTG TAAACCTAGA TTCTCCTATG
    GTATCACCAC CTCCAGACTA TCCTGGAGAA TTCTTTAAAG ATGTTCGACA AGGACTGATA
    GGAGTTGGTC TAATAAATGT AGAAGATCGT TCAGGAATTC TTACTCTAGA TAAAGATTAT
    AACAACATAG GAAAATTCTT AAACAGAATT TTGGGCATGG AAGTGCATCA ACAGAATGCA
    CTATTTCAGT ATTTCGCAGA CACACTTACT GCAGTCGTTC AAAATGCCAA AAAAAATGGA
    AGATACGATA TGGGAATCTT AGATCTTGGT TCTGGGGATG AAAAAGTGAG GAAAAGTGAT
    GTTAAGAAGT TTCTGACTCC AGGATATTCA ACATCTGGCC ATGTAGAATT ATACACAATA
    AGTGTGGAGA GGGGAATGTC CTGGGAGGAA GCTACCAAGA TTTGGGCTGA GCTGACAGGA
    CCAGATGATG GCTTTTATTT GTCATTGCAA ATAAGAAACA ACAAGAAAAC TGCCATCTTA
    GTTAAAGAAG TAAACCCTAA AAAGAAGCTT TTCTTAGTTT ATAGACCAAA TACCGGGAAA
    CAACTCAAAT TAGAAATTTA TGCTGATCTA AAAAAGAAAT ATAAGAAGGT AGTCTCAGAC
    GATGCCGTGG TCCACTGGTT AGATCAGTAT AATTCATCTG CAGATACTTG TACTCATGCT
    TATTGGCGTG GCAACTGCAA AAAAGCAAGC TTGGGATTAG TTTGTGAAAT AGGTCTTCGT
    TGTCGCACAT ACTATGTATT ATGTGGCTCA GTACTGAGTG TCTGGACAAA AGTTGAGGGT
    GTTCTTGCGT CTGTCAGTGG CACAAATGTG AAAATGCAGA TAGTTCGCCT AAGAACAGAG
    GATGGACAGC GGATTGTAGG TTTGATCATT CCTGCAAATT GTGTGTCTCC TCTTATAAAT
    CTCCTTTCAA CTTCAGACCA GTCTCAACAG CTTGCAGTCC AGCAGAAACA GCTCTGGCAG
    CAGCGTCACC CCCAGAGCAT TGCCAGCTTG AGCAGCACAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa08999
    Clone ID Related Accession (Same CDS sequence) XM_002694566.5 , XM_865383.8 , XM_002694566.6
    Accession Version XM_002694566.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4182bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein strawberry notch homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002694566.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGTCAGTGCC ACTTAGTGCA TTAGAACTAG GTTTGGAGAC TGAAGCAGCA
    GTTCCTGTTA AACAAGAACC AGAGACGGTA CCTACTCCAG CACTTTTAAA TGTTAGGCAG
    CAGCCTCCAT CTACTACAAC ATTTGTGCTG AATCAAATAA ATCAGCTTCC AACCTTGGGA
    TCTACAATTG TAATGACTAA GACACCACCT GTAACCACGA ATAGGCAAAC CATCACTTTA
    ACGAAGTTCA TTCAGACTAC TGCAAACACA CGCCCTTCAG TCTCAGCACC AGCAGTACGA
    AATGCCATGA CTTCCGCACC TTCAAAAGAC CAAGTTCAAC TGAAGGACCT GCTAAAAAAT
    AATAGTCTGA ATGAACTCAT GAAACTGAAG CCACCTGCTA ATATTGCTCA GCCAGTTGCA
    ACAGCAGCTA CTGATGTAAG CAATGGCACA GTAAAGAAAG AGTCTTCTAA TAAAGAAGTA
    GCAAGAATAT GGATAAATGA CATGAAAATG AGGAGTTTTT CCCCAACCAT GAAGGTCCCT
    GTTGTGAAAG AGGAAGATGA ACCAGAAGAA GAAGACGAAG AAGAAATGGG TCATGCGGAA
    ACCTATGCAG AGTATATGCC AATAAAATTA AAAATTGGCC TACGTCACCC AGATGCCGTA
    GTTGAGACTA GTTCTTTATC CAGTGTTACT CCTCCTGATG TTTGGTACAA GACATCTATT
    TCTGAAGAAA CCATTGATAA TGGCTGGTTA TCAGCATTAC AGCTTGAAGC AATTACTTAT
    GCAGCCCAGC AACATGAAAC GTTCCTACCT AATGGAGATC GTGCTGGCTT CTTAATAGGC
    GATGGTGCTG GTGTAGGAAA AGGAAGGACA ATAGCAGGAA TCATCTATGA AAATTATTTG
    TTGAGTAGAA AAAGGGCTTT GTGGTTCAGT GTTTCAAATG ATTTAAAGTA TGATGCTGAA
    AGAGATTTGA GGGATATTGG AGCAAAAAAC ATTTTGGTCC ATTCATTGAA TAAGTTTAAA
    TATGGAAAAA TTTCTTCCAA ACATAATGGG AGTGTGAAGA AGGGTGTTAT TTTTGCTACC
    TATTCTTCCC TTATTGGTGA AAGTCAGTCT GGTGGTAAAT ATAAAACGAG GTTAAAACAA
    CTTCTACATT GGTGCGGTGA TGACTTCGAT GGAGTGATAG TTTTTGATGA ATGTCATAAA
    GCAAAAAACT TATGTCCTGT TGGTTCTTCA AAGCCAACCA AGACAGGCTT AGCAGTTTTA
    GAACTTCAGA ACAAATTGCC AAAAGCTAGA GTTGTCTATG CTAGTGCCAC TGGTGCTTCT
    GAACCACGAA ATATGGCCTA TATGAACCGT CTTGGAATAT GGGGTGAGGG AACTCCATTT
    AGAGAATTCA GTGACTTTAT TCAGGCAGTA GAACGGAGAG GTGTTGGTGC CATGGAAATA
    GTTGCTATGG ATATGAAGCT TAGAGGAATG TACATTGCTC GACAGCTGAG CTTTACTGGA
    GTGACCTTCA AAATTGAGGA AGTTCTTCTT TCTCAGAGCT ATGTTAAAAT GTATAACAAA
    GCAGTCAAAC TGTGGGTCAT TGCTCGAGAG CGGTTTCAGC AAGCTGCAGA TCTGATTGAT
    GCTGAGCAGA GAATGAAGAA ATCCATGTGG GGTCAGTTCT GGTCTGCTCA CCAGAGATTT
    TTCAAATACT TGTGCATAGC GTCCAAAGTT AAAAGAGTTG TGCAACTAGC TCGAGAAGAA
    ATCAAGAATG GAAAATGTGT TGTAATTGGT CTGCAGTCTA CGGGAGAAGC TAGAACATTA
    GAAGCCTTGG AAGAGGGCGG GGGAGAATTG AATGATTTTG TTTCAACTGC CAAAGGAGTG
    TTACAGTCAC TCATTGAAAA ACACTTCCCT GCTCCGGATA GAAAAAAGCT TTATAGTTTG
    CTGGGAATTG ATTTGACAGC TCCAAGTAAC AGCAGTTCAC CAAGAGATAG TCCTTGTAAG
    GAAAATAAAA CAAAGAAGCG GAAAGGTGAA GAAATAACTC GAGAAGCCAA AAAAGCACGA
    AAAATAGGTG GCCTTACTGG TAGCAGTTCT GATGATAGTG GAAGTGAATC TGATGCCTCT
    GATAATGAAG AAAGTGACTA TGAGAGCTCT AAAAACATGA GTTCTGGAGA TGATGATGAT
    TTCAACCCAT TCAGAGATGA GTCTAGTGAG GATGATGAAG ATGATCCCTG GTTAATCAGA
    AAAGATCATA AGAAAAACAA AGATAAAAAA AAAAGGAAAA GTATAGATCC AGATTCTATT
    CAAAGTGCCT TATTAGCGTC AGGTCTTGGA TCAAAACGAC CTAGTTTTTC TTCTACACCA
    GTTATTTCAC CTGCTCCTAA CAGTGCACCA GCTAACAGTA ACACCAACAG CAACAGTAGC
    CTTATAACAA GTCAAGATGC CGTGGAAAGG GCCCAACAGA TGAAGAAAGA CCTACTTGAT
    AAACTAGAAA AATTAGCTGA AGACCTCCCT CCTAATACCC TGGATGAACT TATTGATGAA
    CTTGGTGGCC CTGAAAATGT TGCTGAGATG ACTGGCCGCA AGGGGAGGGT CGTAAGCAAT
    GATGACGGAA GCATATCTTA TGAGTCAAGA TCTGAACTTG ATGTTCCTGT GGAAATACTA
    AATATTACAG AAAAACAAAG ATTTATGGAT GGAGATAAGA ATATTGCTAT CATCTCAGAA
    GCTGCCAGCT CAGGTATTTC ATTACAAGCG GATAGAAGGG CTAAAAATCA AAGGCGAAGA
    GTTCATATGA CTCTGGAGTT ACCATGGAGT GCTGATAGAG CAATTCAGCA ATTTGGACGA
    ACTCATAGAT CAAATCAAGT TACCGCTCCT GAATATGTCT TTCTGATTTC TGAATTGGCA
    GGAGAACAAA GATTTGCATC TATTGTTGCT AAAAGACTTG AGAGTTTGGG CGCACTTACA
    CATGGAGACA GAAGAGCAAC AGAATCTAGA GATCTGAGCC GGTTCAACTT CGATAATAAG
    TATGGAAGAA ATGCTTTAGA AATTGTCATG AAATCCATTG TAAACCTAGA TTCTCCTATG
    GTATCACCAC CTCCAGACTA TCCTGGAGAA TTCTTTAAAG ATGTTCGACA AGGACTGATA
    GGAGTTGGTC TAATAAATGT AGAAGATCGT TCAGGAATTC TTACTCTAGA TAAAGATTAT
    AACAACATAG GAAAATTCTT AAACAGAATT TTGGGCATGG AAGTGCATCA ACAGAATGCA
    CTATTTCAGT ATTTCGCAGA CACACTTACT GCAGTCGTTC AAAATGCCAA AAAAAATGGA
    AGATACGATA TGGGAATCTT AGATCTTGGT TCTGGGGATG AAAAAGTGAG GAAAAGTGAT
    GTTAAGAAGT TTCTGACTCC AGGATATTCA ACATCTGGCC ATGTAGAATT ATACACAATA
    AGTGTGGAGA GGGGAATGTC CTGGGAGGAA GCTACCAAGA TTTGGGCTGA GCTGACAGGA
    CCAGATGATG GCTTTTATTT GTCATTGCAA ATAAGAAACA ACAAGAAAAC TGCCATCTTA
    GTTAAAGAAG TAAACCCTAA AAAGAAGCTT TTCTTAGTTT ATAGACCAAA TACCGGGAAA
    CAACTCAAAT TAGAAATTTA TGCTGATCTA AAAAAGAAAT ATAAGAAGGT AGTCTCAGAC
    GATGCCGTGG TCCACTGGTT AGATCAGTAT AATTCATCTG CAGATACTTG TACTCATGCT
    TATTGGCGTG GCAACTGCAA AAAAGCAAGC TTGGGATTAG TTTGTGAAAT AGGTCTTCGT
    TGTCGCACAT ACTATGTATT ATGTGGCTCA GTACTGAGTG TCTGGACAAA AGTTGAGGGT
    GTTCTTGCGT CTGTCAGTGG CACAAATGTG AAAATGCAGA TAGTTCGCCT AAGAACAGAG
    GATGGACAGC GGATTGTAGG TTTGATCATT CCTGCAAATT GTGTGTCTCC TCTTATAAAT
    CTCCTTTCAA CTTCAGACCA GTCTCAACAG CTTGCAGTCC AGCAGAAACA GCTCTGGCAG
    CAGCGTCACC CCCAGAGCAT TGCCAGCTTG AGCAGCACAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694612.2
    CDS166..4347
    Translation

    Target ORF information:

    RefSeq Version XM_002694566.6
    Organism Bos taurus(cattle)
    Definition Bos taurus strawberry notch homolog 1 (SBNO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694566.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGTCAGTGCC ACTTAGTGCA TTAGAACTAG GTTTGGAGAC TGAAGCAGCA
    GTTCCTGTTA AACAAGAACC AGAGACGGTA CCTACTCCAG CACTTTTAAA TGTTAGGCAG
    CAGCCTCCAT CTACTACAAC ATTTGTGCTG AATCAAATAA ATCAGCTTCC AACCTTGGGA
    TCTACAATTG TAATGACTAA GACACCACCT GTAACCACGA ATAGGCAAAC CATCACTTTA
    ACGAAGTTCA TTCAGACTAC TGCAAACACA CGCCCTTCAG TCTCAGCACC AGCAGTACGA
    AATGCCATGA CTTCCGCACC TTCAAAAGAC CAAGTTCAAC TGAAGGACCT GCTAAAAAAT
    AATAGTCTGA ATGAACTCAT GAAACTGAAG CCACCTGCTA ATATTGCTCA GCCAGTTGCA
    ACAGCAGCTA CTGATGTAAG CAATGGCACA GTAAAGAAAG AGTCTTCTAA TAAAGAAGTA
    GCAAGAATAT GGATAAATGA CATGAAAATG AGGAGTTTTT CCCCAACCAT GAAGGTCCCT
    GTTGTGAAAG AGGAAGATGA ACCAGAAGAA GAAGACGAAG AAGAAATGGG TCATGCGGAA
    ACCTATGCAG AGTATATGCC AATAAAATTA AAAATTGGCC TACGTCACCC AGATGCCGTA
    GTTGAGACTA GTTCTTTATC CAGTGTTACT CCTCCTGATG TTTGGTACAA GACATCTATT
    TCTGAAGAAA CCATTGATAA TGGCTGGTTA TCAGCATTAC AGCTTGAAGC AATTACTTAT
    GCAGCCCAGC AACATGAAAC GTTCCTACCT AATGGAGATC GTGCTGGCTT CTTAATAGGC
    GATGGTGCTG GTGTAGGAAA AGGAAGGACA ATAGCAGGAA TCATCTATGA AAATTATTTG
    TTGAGTAGAA AAAGGGCTTT GTGGTTCAGT GTTTCAAATG ATTTAAAGTA TGATGCTGAA
    AGAGATTTGA GGGATATTGG AGCAAAAAAC ATTTTGGTCC ATTCATTGAA TAAGTTTAAA
    TATGGAAAAA TTTCTTCCAA ACATAATGGG AGTGTGAAGA AGGGTGTTAT TTTTGCTACC
    TATTCTTCCC TTATTGGTGA AAGTCAGTCT GGTGGTAAAT ATAAAACGAG GTTAAAACAA
    CTTCTACATT GGTGCGGTGA TGACTTCGAT GGAGTGATAG TTTTTGATGA ATGTCATAAA
    GCAAAAAACT TATGTCCTGT TGGTTCTTCA AAGCCAACCA AGACAGGCTT AGCAGTTTTA
    GAACTTCAGA ACAAATTGCC AAAAGCTAGA GTTGTCTATG CTAGTGCCAC TGGTGCTTCT
    GAACCACGAA ATATGGCCTA TATGAACCGT CTTGGAATAT GGGGTGAGGG AACTCCATTT
    AGAGAATTCA GTGACTTTAT TCAGGCAGTA GAACGGAGAG GTGTTGGTGC CATGGAAATA
    GTTGCTATGG ATATGAAGCT TAGAGGAATG TACATTGCTC GACAGCTGAG CTTTACTGGA
    GTGACCTTCA AAATTGAGGA AGTTCTTCTT TCTCAGAGCT ATGTTAAAAT GTATAACAAA
    GCAGTCAAAC TGTGGGTCAT TGCTCGAGAG CGGTTTCAGC AAGCTGCAGA TCTGATTGAT
    GCTGAGCAGA GAATGAAGAA ATCCATGTGG GGTCAGTTCT GGTCTGCTCA CCAGAGATTT
    TTCAAATACT TGTGCATAGC GTCCAAAGTT AAAAGAGTTG TGCAACTAGC TCGAGAAGAA
    ATCAAGAATG GAAAATGTGT TGTAATTGGT CTGCAGTCTA CGGGAGAAGC TAGAACATTA
    GAAGCCTTGG AAGAGGGCGG GGGAGAATTG AATGATTTTG TTTCAACTGC CAAAGGAGTG
    TTACAGTCAC TCATTGAAAA ACACTTCCCT GCTCCGGATA GAAAAAAGCT TTATAGTTTG
    CTGGGAATTG ATTTGACAGC TCCAAGTAAC AGCAGTTCAC CAAGAGATAG TCCTTGTAAG
    GAAAATAAAA CAAAGAAGCG GAAAGGTGAA GAAATAACTC GAGAAGCCAA AAAAGCACGA
    AAAATAGGTG GCCTTACTGG TAGCAGTTCT GATGATAGTG GAAGTGAATC TGATGCCTCT
    GATAATGAAG AAAGTGACTA TGAGAGCTCT AAAAACATGA GTTCTGGAGA TGATGATGAT
    TTCAACCCAT TCAGAGATGA GTCTAGTGAG GATGATGAAG ATGATCCCTG GTTAATCAGA
    AAAGATCATA AGAAAAACAA AGATAAAAAA AAAAGGAAAA GTATAGATCC AGATTCTATT
    CAAAGTGCCT TATTAGCGTC AGGTCTTGGA TCAAAACGAC CTAGTTTTTC TTCTACACCA
    GTTATTTCAC CTGCTCCTAA CAGTGCACCA GCTAACAGTA ACACCAACAG CAACAGTAGC
    CTTATAACAA GTCAAGATGC CGTGGAAAGG GCCCAACAGA TGAAGAAAGA CCTACTTGAT
    AAACTAGAAA AATTAGCTGA AGACCTCCCT CCTAATACCC TGGATGAACT TATTGATGAA
    CTTGGTGGCC CTGAAAATGT TGCTGAGATG ACTGGCCGCA AGGGGAGGGT CGTAAGCAAT
    GATGACGGAA GCATATCTTA TGAGTCAAGA TCTGAACTTG ATGTTCCTGT GGAAATACTA
    AATATTACAG AAAAACAAAG ATTTATGGAT GGAGATAAGA ATATTGCTAT CATCTCAGAA
    GCTGCCAGCT CAGGTATTTC ATTACAAGCG GATAGAAGGG CTAAAAATCA AAGGCGAAGA
    GTTCATATGA CTCTGGAGTT ACCATGGAGT GCTGATAGAG CAATTCAGCA ATTTGGACGA
    ACTCATAGAT CAAATCAAGT TACCGCTCCT GAATATGTCT TTCTGATTTC TGAATTGGCA
    GGAGAACAAA GATTTGCATC TATTGTTGCT AAAAGACTTG AGAGTTTGGG CGCACTTACA
    CATGGAGACA GAAGAGCAAC AGAATCTAGA GATCTGAGCC GGTTCAACTT CGATAATAAG
    TATGGAAGAA ATGCTTTAGA AATTGTCATG AAATCCATTG TAAACCTAGA TTCTCCTATG
    GTATCACCAC CTCCAGACTA TCCTGGAGAA TTCTTTAAAG ATGTTCGACA AGGACTGATA
    GGAGTTGGTC TAATAAATGT AGAAGATCGT TCAGGAATTC TTACTCTAGA TAAAGATTAT
    AACAACATAG GAAAATTCTT AAACAGAATT TTGGGCATGG AAGTGCATCA ACAGAATGCA
    CTATTTCAGT ATTTCGCAGA CACACTTACT GCAGTCGTTC AAAATGCCAA AAAAAATGGA
    AGATACGATA TGGGAATCTT AGATCTTGGT TCTGGGGATG AAAAAGTGAG GAAAAGTGAT
    GTTAAGAAGT TTCTGACTCC AGGATATTCA ACATCTGGCC ATGTAGAATT ATACACAATA
    AGTGTGGAGA GGGGAATGTC CTGGGAGGAA GCTACCAAGA TTTGGGCTGA GCTGACAGGA
    CCAGATGATG GCTTTTATTT GTCATTGCAA ATAAGAAACA ACAAGAAAAC TGCCATCTTA
    GTTAAAGAAG TAAACCCTAA AAAGAAGCTT TTCTTAGTTT ATAGACCAAA TACCGGGAAA
    CAACTCAAAT TAGAAATTTA TGCTGATCTA AAAAAGAAAT ATAAGAAGGT AGTCTCAGAC
    GATGCCGTGG TCCACTGGTT AGATCAGTAT AATTCATCTG CAGATACTTG TACTCATGCT
    TATTGGCGTG GCAACTGCAA AAAAGCAAGC TTGGGATTAG TTTGTGAAAT AGGTCTTCGT
    TGTCGCACAT ACTATGTATT ATGTGGCTCA GTACTGAGTG TCTGGACAAA AGTTGAGGGT
    GTTCTTGCGT CTGTCAGTGG CACAAATGTG AAAATGCAGA TAGTTCGCCT AAGAACAGAG
    GATGGACAGC GGATTGTAGG TTTGATCATT CCTGCAAATT GTGTGTCTCC TCTTATAAAT
    CTCCTTTCAA CTTCAGACCA GTCTCAACAG CTTGCAGTCC AGCAGAAACA GCTCTGGCAG
    CAGCGTCACC CCCAGAGCAT TGCCAGCTTG AGCAGCACAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09000
    Clone ID Related Accession (Same CDS sequence) XM_005217972.4 , XM_005217972.3 , XM_005195024.3
    Accession Version XM_005217972.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein strawberry notch homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217972.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGTCAGTGCC ACTTAGTGCA TTAGAACTAG GTTTGGAGAC TGAAGCAGCA
    GTTCCTGTTA AACAAGAACC AGAGACGGTA CCTACTCCAG CACTTTTAAA TGTTAGGCAG
    CCTCCATCTA CTACAACATT TGTGCTGAAT CAAATAAATC AGCTTCCAAC CTTGGGATCT
    ACAATTGTAA TGACTAAGAC ACCACCTGTA ACCACGAATA GGCAAACCAT CACTTTAACG
    AAGTTCATTC AGACTACTGC AAACACACGC CCTTCAGTCT CAGCACCAGC AGTACGAAAT
    GCCATGACTT CCGCACCTTC AAAAGACCAA GTTCAACTGA AGGACCTGCT AAAAAATAAT
    AGTCTGAATG AACTCATGAA ACTGAAGCCA CCTGCTAATA TTGCTCAGCC AGTTGCAACA
    GCAGCTACTG ATGTAAGCAA TGGCACAGTA AAGAAAGAGT CTTCTAATAA AGAAGTAGCA
    AGAATATGGA TAAATGACAT GAAAATGAGG AGTTTTTCCC CAACCATGAA GGTCCCTGTT
    GTGAAAGAGG AAGATGAACC AGAAGAAGAA GACGAAGAAG AAATGGGTCA TGCGGAAACC
    TATGCAGAGT ATATGCCAAT AAAATTAAAA ATTGGCCTAC GTCACCCAGA TGCCGTAGTT
    GAGACTAGTT CTTTATCCAG TGTTACTCCT CCTGATGTTT GGTACAAGAC ATCTATTTCT
    GAAGAAACCA TTGATAATGG CTGGTTATCA GCATTACAGC TTGAAGCAAT TACTTATGCA
    GCCCAGCAAC ATGAAACGTT CCTACCTAAT GGAGATCGTG CTGGCTTCTT AATAGGCGAT
    GGTGCTGGTG TAGGAAAAGG AAGGACAATA GCAGGAATCA TCTATGAAAA TTATTTGTTG
    AGTAGAAAAA GGGCTTTGTG GTTCAGTGTT TCAAATGATT TAAAGTATGA TGCTGAAAGA
    GATTTGAGGG ATATTGGAGC AAAAAACATT TTGGTCCATT CATTGAATAA GTTTAAATAT
    GGAAAAATTT CTTCCAAACA TAATGGGAGT GTGAAGAAGG GTGTTATTTT TGCTACCTAT
    TCTTCCCTTA TTGGTGAAAG TCAGTCTGGT GGTAAATATA AAACGAGGTT AAAACAACTT
    CTACATTGGT GCGGTGATGA CTTCGATGGA GTGATAGTTT TTGATGAATG TCATAAAGCA
    AAAAACTTAT GTCCTGTTGG TTCTTCAAAG CCAACCAAGA CAGGCTTAGC AGTTTTAGAA
    CTTCAGAACA AATTGCCAAA AGCTAGAGTT GTCTATGCTA GTGCCACTGG TGCTTCTGAA
    CCACGAAATA TGGCCTATAT GAACCGTCTT GGAATATGGG GTGAGGGAAC TCCATTTAGA
    GAATTCAGTG ACTTTATTCA GGCAGTAGAA CGGAGAGGTG TTGGTGCCAT GGAAATAGTT
    GCTATGGATA TGAAGCTTAG AGGAATGTAC ATTGCTCGAC AGCTGAGCTT TACTGGAGTG
    ACCTTCAAAA TTGAGGAAGT TCTTCTTTCT CAGAGCTATG TTAAAATGTA TAACAAAGCA
    GTCAAACTGT GGGTCATTGC TCGAGAGCGG TTTCAGCAAG CTGCAGATCT GATTGATGCT
    GAGCAGAGAA TGAAGAAATC CATGTGGGGT CAGTTCTGGT CTGCTCACCA GAGATTTTTC
    AAATACTTGT GCATAGCGTC CAAAGTTAAA AGAGTTGTGC AACTAGCTCG AGAAGAAATC
    AAGAATGGAA AATGTGTTGT AATTGGTCTG CAGTCTACGG GAGAAGCTAG AACATTAGAA
    GCCTTGGAAG AGGGCGGGGG AGAATTGAAT GATTTTGTTT CAACTGCCAA AGGAGTGTTA
    CAGTCACTCA TTGAAAAACA CTTCCCTGCT CCGGATAGAA AAAAGCTTTA TAGTTTGCTG
    GGAATTGATT TGACAGCTCC AAGTAACAGC AGTTCACCAA GAGATAGTCC TTGTAAGGAA
    AATAAAACAA AGAAGCGGAA AGGTGAAGAA ATAACTCGAG AAGCCAAAAA AGCACGAAAA
    ATAGGTGGCC TTACTGGTAG CAGTTCTGAT GATAGTGGAA GTGAATCTGA TGCCTCTGAT
    AATGAAGAAA GTGACTATGA GAGCTCTAAA AACATGAGTT CTGGAGATGA TGATGATTTC
    AACCCATTCA GAGATGAGTC TAGTGAGGAT GATGAAGATG ATCCCTGGTT AATCAGAAAA
    GATCATAAGA AAAACAAAGA TAAAAAAAAA AGGAAAAGTA TAGATCCAGA TTCTATTCAA
    AGTGCCTTAT TAGCGTCAGG TCTTGGATCA AAACGACCTA GTTTTTCTTC TACACCAGTT
    ATTTCACCTG CTCCTAACAG TGCACCAGCT AACAGTAACA CCAACAGCAA CAGTAGCCTT
    ATAACAAGTC AAGATGCCGT GGAAAGGGCC CAACAGATGA AGAAAGACCT ACTTGATAAA
    CTAGAAAAAT TAGCTGAAGA CCTCCCTCCT AATACCCTGG ATGAACTTAT TGATGAACTT
    GGTGGCCCTG AAAATGTTGC TGAGATGACT GGCCGCAAGG GGAGGGTCGT AAGCAATGAT
    GACGGAAGCA TATCTTATGA GTCAAGATCT GAACTTGATG TTCCTGTGGA AATACTAAAT
    ATTACAGAAA AACAAAGATT TATGGATGGA GATAAGAATA TTGCTATCAT CTCAGAAGCT
    GCCAGCTCAG GTATTTCATT ACAAGCGGAT AGAAGGGCTA AAAATCAAAG GCGAAGAGTT
    CATATGACTC TGGAGTTACC ATGGAGTGCT GATAGAGCAA TTCAGCAATT TGGACGAACT
    CATAGATCAA ATCAAGTTAC CGCTCCTGAA TATGTCTTTC TGATTTCTGA ATTGGCAGGA
    GAACAAAGAT TTGCATCTAT TGTTGCTAAA AGACTTGAGA GTTTGGGCGC ACTTACACAT
    GGAGACAGAA GAGCAACAGA ATCTAGAGAT CTGAGCCGGT TCAACTTCGA TAATAAGTAT
    GGAAGAAATG CTTTAGAAAT TGTCATGAAA TCCATTGTAA ACCTAGATTC TCCTATGGTA
    TCACCACCTC CAGACTATCC TGGAGAATTC TTTAAAGATG TTCGACAAGG ACTGATAGGA
    GTTGGTCTAA TAAATGTAGA AGATCGTTCA GGAATTCTTA CTCTAGATAA AGATTATAAC
    AACATAGGAA AATTCTTAAA CAGAATTTTG GGCATGGAAG TGCATCAACA GAATGCACTA
    TTTCAGTATT TCGCAGACAC ACTTACTGCA GTCGTTCAAA ATGCCAAAAA AAATGGAAGA
    TACGATATGG GAATCTTAGA TCTTGGTTCT GGGGATGAAA AAGTGAGGAA AAGTGATGTT
    AAGAAGTTTC TGACTCCAGG ATATTCAACA TCTGGCCATG TAGAATTATA CACAATAAGT
    GTGGAGAGGG GAATGTCCTG GGAGGAAGCT ACCAAGATTT GGGCTGAGCT GACAGGACCA
    GATGATGGCT TTTATTTGTC ATTGCAAATA AGAAACAACA AGAAAACTGC CATCTTAGTT
    AAAGAAGTAA ACCCTAAAAA GAAGCTTTTC TTAGTTTATA GACCAAATAC CGGGAAACAA
    CTCAAATTAG AAATTTATGC TGATCTAAAA AAGAAATATA AGAAGGTAGT CTCAGACGAT
    GCCGTGGTCC ACTGGTTAGA TCAGTATAAT TCATCTGCAG ATACTTGTAC TCATGCTTAT
    TGGCGTGGCA ACTGCAAAAA AGCAAGCTTG GGATTAGTTT GTGAAATAGG TCTTCGTTGT
    CGCACATACT ATGTATTATG TGGCTCAGTA CTGAGTGTCT GGACAAAAGT TGAGGGTGTT
    CTTGCGTCTG TCAGTGGCAC AAATGTGAAA ATGCAGATAG TTCGCCTAAG AACAGAGGAT
    GGACAGCGGA TTGTAGGTTT GATCATTCCT GCAAATTGTG TGTCTCCTCT TATAAATCTC
    CTTTCAACTT CAGACCAGTC TCAACAGCTT GCAGTCCAGC AGAAACAGCT CTGGCAGCAG
    CGTCACCCCC AGAGCATTGC CAGCTTGAGC AGCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218029.1
    CDS115..4293
    Translation

    Target ORF information:

    RefSeq Version XM_005217972.4
    Organism Bos taurus(cattle)
    Definition Bos taurus strawberry notch homolog 1 (SBNO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217972.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGTCAGTGCC ACTTAGTGCA TTAGAACTAG GTTTGGAGAC TGAAGCAGCA
    GTTCCTGTTA AACAAGAACC AGAGACGGTA CCTACTCCAG CACTTTTAAA TGTTAGGCAG
    CCTCCATCTA CTACAACATT TGTGCTGAAT CAAATAAATC AGCTTCCAAC CTTGGGATCT
    ACAATTGTAA TGACTAAGAC ACCACCTGTA ACCACGAATA GGCAAACCAT CACTTTAACG
    AAGTTCATTC AGACTACTGC AAACACACGC CCTTCAGTCT CAGCACCAGC AGTACGAAAT
    GCCATGACTT CCGCACCTTC AAAAGACCAA GTTCAACTGA AGGACCTGCT AAAAAATAAT
    AGTCTGAATG AACTCATGAA ACTGAAGCCA CCTGCTAATA TTGCTCAGCC AGTTGCAACA
    GCAGCTACTG ATGTAAGCAA TGGCACAGTA AAGAAAGAGT CTTCTAATAA AGAAGTAGCA
    AGAATATGGA TAAATGACAT GAAAATGAGG AGTTTTTCCC CAACCATGAA GGTCCCTGTT
    GTGAAAGAGG AAGATGAACC AGAAGAAGAA GACGAAGAAG AAATGGGTCA TGCGGAAACC
    TATGCAGAGT ATATGCCAAT AAAATTAAAA ATTGGCCTAC GTCACCCAGA TGCCGTAGTT
    GAGACTAGTT CTTTATCCAG TGTTACTCCT CCTGATGTTT GGTACAAGAC ATCTATTTCT
    GAAGAAACCA TTGATAATGG CTGGTTATCA GCATTACAGC TTGAAGCAAT TACTTATGCA
    GCCCAGCAAC ATGAAACGTT CCTACCTAAT GGAGATCGTG CTGGCTTCTT AATAGGCGAT
    GGTGCTGGTG TAGGAAAAGG AAGGACAATA GCAGGAATCA TCTATGAAAA TTATTTGTTG
    AGTAGAAAAA GGGCTTTGTG GTTCAGTGTT TCAAATGATT TAAAGTATGA TGCTGAAAGA
    GATTTGAGGG ATATTGGAGC AAAAAACATT TTGGTCCATT CATTGAATAA GTTTAAATAT
    GGAAAAATTT CTTCCAAACA TAATGGGAGT GTGAAGAAGG GTGTTATTTT TGCTACCTAT
    TCTTCCCTTA TTGGTGAAAG TCAGTCTGGT GGTAAATATA AAACGAGGTT AAAACAACTT
    CTACATTGGT GCGGTGATGA CTTCGATGGA GTGATAGTTT TTGATGAATG TCATAAAGCA
    AAAAACTTAT GTCCTGTTGG TTCTTCAAAG CCAACCAAGA CAGGCTTAGC AGTTTTAGAA
    CTTCAGAACA AATTGCCAAA AGCTAGAGTT GTCTATGCTA GTGCCACTGG TGCTTCTGAA
    CCACGAAATA TGGCCTATAT GAACCGTCTT GGAATATGGG GTGAGGGAAC TCCATTTAGA
    GAATTCAGTG ACTTTATTCA GGCAGTAGAA CGGAGAGGTG TTGGTGCCAT GGAAATAGTT
    GCTATGGATA TGAAGCTTAG AGGAATGTAC ATTGCTCGAC AGCTGAGCTT TACTGGAGTG
    ACCTTCAAAA TTGAGGAAGT TCTTCTTTCT CAGAGCTATG TTAAAATGTA TAACAAAGCA
    GTCAAACTGT GGGTCATTGC TCGAGAGCGG TTTCAGCAAG CTGCAGATCT GATTGATGCT
    GAGCAGAGAA TGAAGAAATC CATGTGGGGT CAGTTCTGGT CTGCTCACCA GAGATTTTTC
    AAATACTTGT GCATAGCGTC CAAAGTTAAA AGAGTTGTGC AACTAGCTCG AGAAGAAATC
    AAGAATGGAA AATGTGTTGT AATTGGTCTG CAGTCTACGG GAGAAGCTAG AACATTAGAA
    GCCTTGGAAG AGGGCGGGGG AGAATTGAAT GATTTTGTTT CAACTGCCAA AGGAGTGTTA
    CAGTCACTCA TTGAAAAACA CTTCCCTGCT CCGGATAGAA AAAAGCTTTA TAGTTTGCTG
    GGAATTGATT TGACAGCTCC AAGTAACAGC AGTTCACCAA GAGATAGTCC TTGTAAGGAA
    AATAAAACAA AGAAGCGGAA AGGTGAAGAA ATAACTCGAG AAGCCAAAAA AGCACGAAAA
    ATAGGTGGCC TTACTGGTAG CAGTTCTGAT GATAGTGGAA GTGAATCTGA TGCCTCTGAT
    AATGAAGAAA GTGACTATGA GAGCTCTAAA AACATGAGTT CTGGAGATGA TGATGATTTC
    AACCCATTCA GAGATGAGTC TAGTGAGGAT GATGAAGATG ATCCCTGGTT AATCAGAAAA
    GATCATAAGA AAAACAAAGA TAAAAAAAAA AGGAAAAGTA TAGATCCAGA TTCTATTCAA
    AGTGCCTTAT TAGCGTCAGG TCTTGGATCA AAACGACCTA GTTTTTCTTC TACACCAGTT
    ATTTCACCTG CTCCTAACAG TGCACCAGCT AACAGTAACA CCAACAGCAA CAGTAGCCTT
    ATAACAAGTC AAGATGCCGT GGAAAGGGCC CAACAGATGA AGAAAGACCT ACTTGATAAA
    CTAGAAAAAT TAGCTGAAGA CCTCCCTCCT AATACCCTGG ATGAACTTAT TGATGAACTT
    GGTGGCCCTG AAAATGTTGC TGAGATGACT GGCCGCAAGG GGAGGGTCGT AAGCAATGAT
    GACGGAAGCA TATCTTATGA GTCAAGATCT GAACTTGATG TTCCTGTGGA AATACTAAAT
    ATTACAGAAA AACAAAGATT TATGGATGGA GATAAGAATA TTGCTATCAT CTCAGAAGCT
    GCCAGCTCAG GTATTTCATT ACAAGCGGAT AGAAGGGCTA AAAATCAAAG GCGAAGAGTT
    CATATGACTC TGGAGTTACC ATGGAGTGCT GATAGAGCAA TTCAGCAATT TGGACGAACT
    CATAGATCAA ATCAAGTTAC CGCTCCTGAA TATGTCTTTC TGATTTCTGA ATTGGCAGGA
    GAACAAAGAT TTGCATCTAT TGTTGCTAAA AGACTTGAGA GTTTGGGCGC ACTTACACAT
    GGAGACAGAA GAGCAACAGA ATCTAGAGAT CTGAGCCGGT TCAACTTCGA TAATAAGTAT
    GGAAGAAATG CTTTAGAAAT TGTCATGAAA TCCATTGTAA ACCTAGATTC TCCTATGGTA
    TCACCACCTC CAGACTATCC TGGAGAATTC TTTAAAGATG TTCGACAAGG ACTGATAGGA
    GTTGGTCTAA TAAATGTAGA AGATCGTTCA GGAATTCTTA CTCTAGATAA AGATTATAAC
    AACATAGGAA AATTCTTAAA CAGAATTTTG GGCATGGAAG TGCATCAACA GAATGCACTA
    TTTCAGTATT TCGCAGACAC ACTTACTGCA GTCGTTCAAA ATGCCAAAAA AAATGGAAGA
    TACGATATGG GAATCTTAGA TCTTGGTTCT GGGGATGAAA AAGTGAGGAA AAGTGATGTT
    AAGAAGTTTC TGACTCCAGG ATATTCAACA TCTGGCCATG TAGAATTATA CACAATAAGT
    GTGGAGAGGG GAATGTCCTG GGAGGAAGCT ACCAAGATTT GGGCTGAGCT GACAGGACCA
    GATGATGGCT TTTATTTGTC ATTGCAAATA AGAAACAACA AGAAAACTGC CATCTTAGTT
    AAAGAAGTAA ACCCTAAAAA GAAGCTTTTC TTAGTTTATA GACCAAATAC CGGGAAACAA
    CTCAAATTAG AAATTTATGC TGATCTAAAA AAGAAATATA AGAAGGTAGT CTCAGACGAT
    GCCGTGGTCC ACTGGTTAGA TCAGTATAAT TCATCTGCAG ATACTTGTAC TCATGCTTAT
    TGGCGTGGCA ACTGCAAAAA AGCAAGCTTG GGATTAGTTT GTGAAATAGG TCTTCGTTGT
    CGCACATACT ATGTATTATG TGGCTCAGTA CTGAGTGTCT GGACAAAAGT TGAGGGTGTT
    CTTGCGTCTG TCAGTGGCAC AAATGTGAAA ATGCAGATAG TTCGCCTAAG AACAGAGGAT
    GGACAGCGGA TTGTAGGTTT GATCATTCCT GCAAATTGTG TGTCTCCTCT TATAAATCTC
    CTTTCAACTT CAGACCAGTC TCAACAGCTT GCAGTCCAGC AGAAACAGCT CTGGCAGCAG
    CGTCACCCCC AGAGCATTGC CAGCTTGAGC AGCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09000
    Clone ID Related Accession (Same CDS sequence) XM_005217972.4 , XM_005217972.3 , XM_005195024.3
    Accession Version XM_005217972.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein strawberry notch homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217972.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGTCAGTGCC ACTTAGTGCA TTAGAACTAG GTTTGGAGAC TGAAGCAGCA
    GTTCCTGTTA AACAAGAACC AGAGACGGTA CCTACTCCAG CACTTTTAAA TGTTAGGCAG
    CCTCCATCTA CTACAACATT TGTGCTGAAT CAAATAAATC AGCTTCCAAC CTTGGGATCT
    ACAATTGTAA TGACTAAGAC ACCACCTGTA ACCACGAATA GGCAAACCAT CACTTTAACG
    AAGTTCATTC AGACTACTGC AAACACACGC CCTTCAGTCT CAGCACCAGC AGTACGAAAT
    GCCATGACTT CCGCACCTTC AAAAGACCAA GTTCAACTGA AGGACCTGCT AAAAAATAAT
    AGTCTGAATG AACTCATGAA ACTGAAGCCA CCTGCTAATA TTGCTCAGCC AGTTGCAACA
    GCAGCTACTG ATGTAAGCAA TGGCACAGTA AAGAAAGAGT CTTCTAATAA AGAAGTAGCA
    AGAATATGGA TAAATGACAT GAAAATGAGG AGTTTTTCCC CAACCATGAA GGTCCCTGTT
    GTGAAAGAGG AAGATGAACC AGAAGAAGAA GACGAAGAAG AAATGGGTCA TGCGGAAACC
    TATGCAGAGT ATATGCCAAT AAAATTAAAA ATTGGCCTAC GTCACCCAGA TGCCGTAGTT
    GAGACTAGTT CTTTATCCAG TGTTACTCCT CCTGATGTTT GGTACAAGAC ATCTATTTCT
    GAAGAAACCA TTGATAATGG CTGGTTATCA GCATTACAGC TTGAAGCAAT TACTTATGCA
    GCCCAGCAAC ATGAAACGTT CCTACCTAAT GGAGATCGTG CTGGCTTCTT AATAGGCGAT
    GGTGCTGGTG TAGGAAAAGG AAGGACAATA GCAGGAATCA TCTATGAAAA TTATTTGTTG
    AGTAGAAAAA GGGCTTTGTG GTTCAGTGTT TCAAATGATT TAAAGTATGA TGCTGAAAGA
    GATTTGAGGG ATATTGGAGC AAAAAACATT TTGGTCCATT CATTGAATAA GTTTAAATAT
    GGAAAAATTT CTTCCAAACA TAATGGGAGT GTGAAGAAGG GTGTTATTTT TGCTACCTAT
    TCTTCCCTTA TTGGTGAAAG TCAGTCTGGT GGTAAATATA AAACGAGGTT AAAACAACTT
    CTACATTGGT GCGGTGATGA CTTCGATGGA GTGATAGTTT TTGATGAATG TCATAAAGCA
    AAAAACTTAT GTCCTGTTGG TTCTTCAAAG CCAACCAAGA CAGGCTTAGC AGTTTTAGAA
    CTTCAGAACA AATTGCCAAA AGCTAGAGTT GTCTATGCTA GTGCCACTGG TGCTTCTGAA
    CCACGAAATA TGGCCTATAT GAACCGTCTT GGAATATGGG GTGAGGGAAC TCCATTTAGA
    GAATTCAGTG ACTTTATTCA GGCAGTAGAA CGGAGAGGTG TTGGTGCCAT GGAAATAGTT
    GCTATGGATA TGAAGCTTAG AGGAATGTAC ATTGCTCGAC AGCTGAGCTT TACTGGAGTG
    ACCTTCAAAA TTGAGGAAGT TCTTCTTTCT CAGAGCTATG TTAAAATGTA TAACAAAGCA
    GTCAAACTGT GGGTCATTGC TCGAGAGCGG TTTCAGCAAG CTGCAGATCT GATTGATGCT
    GAGCAGAGAA TGAAGAAATC CATGTGGGGT CAGTTCTGGT CTGCTCACCA GAGATTTTTC
    AAATACTTGT GCATAGCGTC CAAAGTTAAA AGAGTTGTGC AACTAGCTCG AGAAGAAATC
    AAGAATGGAA AATGTGTTGT AATTGGTCTG CAGTCTACGG GAGAAGCTAG AACATTAGAA
    GCCTTGGAAG AGGGCGGGGG AGAATTGAAT GATTTTGTTT CAACTGCCAA AGGAGTGTTA
    CAGTCACTCA TTGAAAAACA CTTCCCTGCT CCGGATAGAA AAAAGCTTTA TAGTTTGCTG
    GGAATTGATT TGACAGCTCC AAGTAACAGC AGTTCACCAA GAGATAGTCC TTGTAAGGAA
    AATAAAACAA AGAAGCGGAA AGGTGAAGAA ATAACTCGAG AAGCCAAAAA AGCACGAAAA
    ATAGGTGGCC TTACTGGTAG CAGTTCTGAT GATAGTGGAA GTGAATCTGA TGCCTCTGAT
    AATGAAGAAA GTGACTATGA GAGCTCTAAA AACATGAGTT CTGGAGATGA TGATGATTTC
    AACCCATTCA GAGATGAGTC TAGTGAGGAT GATGAAGATG ATCCCTGGTT AATCAGAAAA
    GATCATAAGA AAAACAAAGA TAAAAAAAAA AGGAAAAGTA TAGATCCAGA TTCTATTCAA
    AGTGCCTTAT TAGCGTCAGG TCTTGGATCA AAACGACCTA GTTTTTCTTC TACACCAGTT
    ATTTCACCTG CTCCTAACAG TGCACCAGCT AACAGTAACA CCAACAGCAA CAGTAGCCTT
    ATAACAAGTC AAGATGCCGT GGAAAGGGCC CAACAGATGA AGAAAGACCT ACTTGATAAA
    CTAGAAAAAT TAGCTGAAGA CCTCCCTCCT AATACCCTGG ATGAACTTAT TGATGAACTT
    GGTGGCCCTG AAAATGTTGC TGAGATGACT GGCCGCAAGG GGAGGGTCGT AAGCAATGAT
    GACGGAAGCA TATCTTATGA GTCAAGATCT GAACTTGATG TTCCTGTGGA AATACTAAAT
    ATTACAGAAA AACAAAGATT TATGGATGGA GATAAGAATA TTGCTATCAT CTCAGAAGCT
    GCCAGCTCAG GTATTTCATT ACAAGCGGAT AGAAGGGCTA AAAATCAAAG GCGAAGAGTT
    CATATGACTC TGGAGTTACC ATGGAGTGCT GATAGAGCAA TTCAGCAATT TGGACGAACT
    CATAGATCAA ATCAAGTTAC CGCTCCTGAA TATGTCTTTC TGATTTCTGA ATTGGCAGGA
    GAACAAAGAT TTGCATCTAT TGTTGCTAAA AGACTTGAGA GTTTGGGCGC ACTTACACAT
    GGAGACAGAA GAGCAACAGA ATCTAGAGAT CTGAGCCGGT TCAACTTCGA TAATAAGTAT
    GGAAGAAATG CTTTAGAAAT TGTCATGAAA TCCATTGTAA ACCTAGATTC TCCTATGGTA
    TCACCACCTC CAGACTATCC TGGAGAATTC TTTAAAGATG TTCGACAAGG ACTGATAGGA
    GTTGGTCTAA TAAATGTAGA AGATCGTTCA GGAATTCTTA CTCTAGATAA AGATTATAAC
    AACATAGGAA AATTCTTAAA CAGAATTTTG GGCATGGAAG TGCATCAACA GAATGCACTA
    TTTCAGTATT TCGCAGACAC ACTTACTGCA GTCGTTCAAA ATGCCAAAAA AAATGGAAGA
    TACGATATGG GAATCTTAGA TCTTGGTTCT GGGGATGAAA AAGTGAGGAA AAGTGATGTT
    AAGAAGTTTC TGACTCCAGG ATATTCAACA TCTGGCCATG TAGAATTATA CACAATAAGT
    GTGGAGAGGG GAATGTCCTG GGAGGAAGCT ACCAAGATTT GGGCTGAGCT GACAGGACCA
    GATGATGGCT TTTATTTGTC ATTGCAAATA AGAAACAACA AGAAAACTGC CATCTTAGTT
    AAAGAAGTAA ACCCTAAAAA GAAGCTTTTC TTAGTTTATA GACCAAATAC CGGGAAACAA
    CTCAAATTAG AAATTTATGC TGATCTAAAA AAGAAATATA AGAAGGTAGT CTCAGACGAT
    GCCGTGGTCC ACTGGTTAGA TCAGTATAAT TCATCTGCAG ATACTTGTAC TCATGCTTAT
    TGGCGTGGCA ACTGCAAAAA AGCAAGCTTG GGATTAGTTT GTGAAATAGG TCTTCGTTGT
    CGCACATACT ATGTATTATG TGGCTCAGTA CTGAGTGTCT GGACAAAAGT TGAGGGTGTT
    CTTGCGTCTG TCAGTGGCAC AAATGTGAAA ATGCAGATAG TTCGCCTAAG AACAGAGGAT
    GGACAGCGGA TTGTAGGTTT GATCATTCCT GCAAATTGTG TGTCTCCTCT TATAAATCTC
    CTTTCAACTT CAGACCAGTC TCAACAGCTT GCAGTCCAGC AGAAACAGCT CTGGCAGCAG
    CGTCACCCCC AGAGCATTGC CAGCTTGAGC AGCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218029.1
    CDS136..4314
    Translation

    Target ORF information:

    RefSeq Version XM_005217972.3
    Organism Bos taurus(cattle)
    Definition Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217972.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGTCAGTGCC ACTTAGTGCA TTAGAACTAG GTTTGGAGAC TGAAGCAGCA
    GTTCCTGTTA AACAAGAACC AGAGACGGTA CCTACTCCAG CACTTTTAAA TGTTAGGCAG
    CCTCCATCTA CTACAACATT TGTGCTGAAT CAAATAAATC AGCTTCCAAC CTTGGGATCT
    ACAATTGTAA TGACTAAGAC ACCACCTGTA ACCACGAATA GGCAAACCAT CACTTTAACG
    AAGTTCATTC AGACTACTGC AAACACACGC CCTTCAGTCT CAGCACCAGC AGTACGAAAT
    GCCATGACTT CCGCACCTTC AAAAGACCAA GTTCAACTGA AGGACCTGCT AAAAAATAAT
    AGTCTGAATG AACTCATGAA ACTGAAGCCA CCTGCTAATA TTGCTCAGCC AGTTGCAACA
    GCAGCTACTG ATGTAAGCAA TGGCACAGTA AAGAAAGAGT CTTCTAATAA AGAAGTAGCA
    AGAATATGGA TAAATGACAT GAAAATGAGG AGTTTTTCCC CAACCATGAA GGTCCCTGTT
    GTGAAAGAGG AAGATGAACC AGAAGAAGAA GACGAAGAAG AAATGGGTCA TGCGGAAACC
    TATGCAGAGT ATATGCCAAT AAAATTAAAA ATTGGCCTAC GTCACCCAGA TGCCGTAGTT
    GAGACTAGTT CTTTATCCAG TGTTACTCCT CCTGATGTTT GGTACAAGAC ATCTATTTCT
    GAAGAAACCA TTGATAATGG CTGGTTATCA GCATTACAGC TTGAAGCAAT TACTTATGCA
    GCCCAGCAAC ATGAAACGTT CCTACCTAAT GGAGATCGTG CTGGCTTCTT AATAGGCGAT
    GGTGCTGGTG TAGGAAAAGG AAGGACAATA GCAGGAATCA TCTATGAAAA TTATTTGTTG
    AGTAGAAAAA GGGCTTTGTG GTTCAGTGTT TCAAATGATT TAAAGTATGA TGCTGAAAGA
    GATTTGAGGG ATATTGGAGC AAAAAACATT TTGGTCCATT CATTGAATAA GTTTAAATAT
    GGAAAAATTT CTTCCAAACA TAATGGGAGT GTGAAGAAGG GTGTTATTTT TGCTACCTAT
    TCTTCCCTTA TTGGTGAAAG TCAGTCTGGT GGTAAATATA AAACGAGGTT AAAACAACTT
    CTACATTGGT GCGGTGATGA CTTCGATGGA GTGATAGTTT TTGATGAATG TCATAAAGCA
    AAAAACTTAT GTCCTGTTGG TTCTTCAAAG CCAACCAAGA CAGGCTTAGC AGTTTTAGAA
    CTTCAGAACA AATTGCCAAA AGCTAGAGTT GTCTATGCTA GTGCCACTGG TGCTTCTGAA
    CCACGAAATA TGGCCTATAT GAACCGTCTT GGAATATGGG GTGAGGGAAC TCCATTTAGA
    GAATTCAGTG ACTTTATTCA GGCAGTAGAA CGGAGAGGTG TTGGTGCCAT GGAAATAGTT
    GCTATGGATA TGAAGCTTAG AGGAATGTAC ATTGCTCGAC AGCTGAGCTT TACTGGAGTG
    ACCTTCAAAA TTGAGGAAGT TCTTCTTTCT CAGAGCTATG TTAAAATGTA TAACAAAGCA
    GTCAAACTGT GGGTCATTGC TCGAGAGCGG TTTCAGCAAG CTGCAGATCT GATTGATGCT
    GAGCAGAGAA TGAAGAAATC CATGTGGGGT CAGTTCTGGT CTGCTCACCA GAGATTTTTC
    AAATACTTGT GCATAGCGTC CAAAGTTAAA AGAGTTGTGC AACTAGCTCG AGAAGAAATC
    AAGAATGGAA AATGTGTTGT AATTGGTCTG CAGTCTACGG GAGAAGCTAG AACATTAGAA
    GCCTTGGAAG AGGGCGGGGG AGAATTGAAT GATTTTGTTT CAACTGCCAA AGGAGTGTTA
    CAGTCACTCA TTGAAAAACA CTTCCCTGCT CCGGATAGAA AAAAGCTTTA TAGTTTGCTG
    GGAATTGATT TGACAGCTCC AAGTAACAGC AGTTCACCAA GAGATAGTCC TTGTAAGGAA
    AATAAAACAA AGAAGCGGAA AGGTGAAGAA ATAACTCGAG AAGCCAAAAA AGCACGAAAA
    ATAGGTGGCC TTACTGGTAG CAGTTCTGAT GATAGTGGAA GTGAATCTGA TGCCTCTGAT
    AATGAAGAAA GTGACTATGA GAGCTCTAAA AACATGAGTT CTGGAGATGA TGATGATTTC
    AACCCATTCA GAGATGAGTC TAGTGAGGAT GATGAAGATG ATCCCTGGTT AATCAGAAAA
    GATCATAAGA AAAACAAAGA TAAAAAAAAA AGGAAAAGTA TAGATCCAGA TTCTATTCAA
    AGTGCCTTAT TAGCGTCAGG TCTTGGATCA AAACGACCTA GTTTTTCTTC TACACCAGTT
    ATTTCACCTG CTCCTAACAG TGCACCAGCT AACAGTAACA CCAACAGCAA CAGTAGCCTT
    ATAACAAGTC AAGATGCCGT GGAAAGGGCC CAACAGATGA AGAAAGACCT ACTTGATAAA
    CTAGAAAAAT TAGCTGAAGA CCTCCCTCCT AATACCCTGG ATGAACTTAT TGATGAACTT
    GGTGGCCCTG AAAATGTTGC TGAGATGACT GGCCGCAAGG GGAGGGTCGT AAGCAATGAT
    GACGGAAGCA TATCTTATGA GTCAAGATCT GAACTTGATG TTCCTGTGGA AATACTAAAT
    ATTACAGAAA AACAAAGATT TATGGATGGA GATAAGAATA TTGCTATCAT CTCAGAAGCT
    GCCAGCTCAG GTATTTCATT ACAAGCGGAT AGAAGGGCTA AAAATCAAAG GCGAAGAGTT
    CATATGACTC TGGAGTTACC ATGGAGTGCT GATAGAGCAA TTCAGCAATT TGGACGAACT
    CATAGATCAA ATCAAGTTAC CGCTCCTGAA TATGTCTTTC TGATTTCTGA ATTGGCAGGA
    GAACAAAGAT TTGCATCTAT TGTTGCTAAA AGACTTGAGA GTTTGGGCGC ACTTACACAT
    GGAGACAGAA GAGCAACAGA ATCTAGAGAT CTGAGCCGGT TCAACTTCGA TAATAAGTAT
    GGAAGAAATG CTTTAGAAAT TGTCATGAAA TCCATTGTAA ACCTAGATTC TCCTATGGTA
    TCACCACCTC CAGACTATCC TGGAGAATTC TTTAAAGATG TTCGACAAGG ACTGATAGGA
    GTTGGTCTAA TAAATGTAGA AGATCGTTCA GGAATTCTTA CTCTAGATAA AGATTATAAC
    AACATAGGAA AATTCTTAAA CAGAATTTTG GGCATGGAAG TGCATCAACA GAATGCACTA
    TTTCAGTATT TCGCAGACAC ACTTACTGCA GTCGTTCAAA ATGCCAAAAA AAATGGAAGA
    TACGATATGG GAATCTTAGA TCTTGGTTCT GGGGATGAAA AAGTGAGGAA AAGTGATGTT
    AAGAAGTTTC TGACTCCAGG ATATTCAACA TCTGGCCATG TAGAATTATA CACAATAAGT
    GTGGAGAGGG GAATGTCCTG GGAGGAAGCT ACCAAGATTT GGGCTGAGCT GACAGGACCA
    GATGATGGCT TTTATTTGTC ATTGCAAATA AGAAACAACA AGAAAACTGC CATCTTAGTT
    AAAGAAGTAA ACCCTAAAAA GAAGCTTTTC TTAGTTTATA GACCAAATAC CGGGAAACAA
    CTCAAATTAG AAATTTATGC TGATCTAAAA AAGAAATATA AGAAGGTAGT CTCAGACGAT
    GCCGTGGTCC ACTGGTTAGA TCAGTATAAT TCATCTGCAG ATACTTGTAC TCATGCTTAT
    TGGCGTGGCA ACTGCAAAAA AGCAAGCTTG GGATTAGTTT GTGAAATAGG TCTTCGTTGT
    CGCACATACT ATGTATTATG TGGCTCAGTA CTGAGTGTCT GGACAAAAGT TGAGGGTGTT
    CTTGCGTCTG TCAGTGGCAC AAATGTGAAA ATGCAGATAG TTCGCCTAAG AACAGAGGAT
    GGACAGCGGA TTGTAGGTTT GATCATTCCT GCAAATTGTG TGTCTCCTCT TATAAATCTC
    CTTTCAACTT CAGACCAGTC TCAACAGCTT GCAGTCCAGC AGAAACAGCT CTGGCAGCAG
    CGTCACCCCC AGAGCATTGC CAGCTTGAGC AGCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09000
    Clone ID Related Accession (Same CDS sequence) XM_005217972.4 , XM_005217972.3 , XM_005195024.3
    Accession Version XM_005195024.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein strawberry notch homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195024.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGTCAGTGCC ACTTAGTGCA TTAGAACTAG GTTTGGAGAC TGAAGCAGCA
    GTTCCTGTTA AACAAGAACC AGAGACGGTA CCTACTCCAG CACTTTTAAA TGTTAGGCAG
    CCTCCATCTA CTACAACATT TGTGCTGAAT CAAATAAATC AGCTTCCAAC CTTGGGATCT
    ACAATTGTAA TGACTAAGAC ACCACCTGTA ACCACGAATA GGCAAACCAT CACTTTAACG
    AAGTTCATTC AGACTACTGC AAACACACGC CCTTCAGTCT CAGCACCAGC AGTACGAAAT
    GCCATGACTT CCGCACCTTC AAAAGACCAA GTTCAACTGA AGGACCTGCT AAAAAATAAT
    AGTCTGAATG AACTCATGAA ACTGAAGCCA CCTGCTAATA TTGCTCAGCC AGTTGCAACA
    GCAGCTACTG ATGTAAGCAA TGGCACAGTA AAGAAAGAGT CTTCTAATAA AGAAGTAGCA
    AGAATATGGA TAAATGACAT GAAAATGAGG AGTTTTTCCC CAACCATGAA GGTCCCTGTT
    GTGAAAGAGG AAGATGAACC AGAAGAAGAA GACGAAGAAG AAATGGGTCA TGCGGAAACC
    TATGCAGAGT ATATGCCAAT AAAATTAAAA ATTGGCCTAC GTCACCCAGA TGCCGTAGTT
    GAGACTAGTT CTTTATCCAG TGTTACTCCT CCTGATGTTT GGTACAAGAC ATCTATTTCT
    GAAGAAACCA TTGATAATGG CTGGTTATCA GCATTACAGC TTGAAGCAAT TACTTATGCA
    GCCCAGCAAC ATGAAACGTT CCTACCTAAT GGAGATCGTG CTGGCTTCTT AATAGGCGAT
    GGTGCTGGTG TAGGAAAAGG AAGGACAATA GCAGGAATCA TCTATGAAAA TTATTTGTTG
    AGTAGAAAAA GGGCTTTGTG GTTCAGTGTT TCAAATGATT TAAAGTATGA TGCTGAAAGA
    GATTTGAGGG ATATTGGAGC AAAAAACATT TTGGTCCATT CATTGAATAA GTTTAAATAT
    GGAAAAATTT CTTCCAAACA TAATGGGAGT GTGAAGAAGG GTGTTATTTT TGCTACCTAT
    TCTTCCCTTA TTGGTGAAAG TCAGTCTGGT GGTAAATATA AAACGAGGTT AAAACAACTT
    CTACATTGGT GCGGTGATGA CTTCGATGGA GTGATAGTTT TTGATGAATG TCATAAAGCA
    AAAAACTTAT GTCCTGTTGG TTCTTCAAAG CCAACCAAGA CAGGCTTAGC AGTTTTAGAA
    CTTCAGAACA AATTGCCAAA AGCTAGAGTT GTCTATGCTA GTGCCACTGG TGCTTCTGAA
    CCACGAAATA TGGCCTATAT GAACCGTCTT GGAATATGGG GTGAGGGAAC TCCATTTAGA
    GAATTCAGTG ACTTTATTCA GGCAGTAGAA CGGAGAGGTG TTGGTGCCAT GGAAATAGTT
    GCTATGGATA TGAAGCTTAG AGGAATGTAC ATTGCTCGAC AGCTGAGCTT TACTGGAGTG
    ACCTTCAAAA TTGAGGAAGT TCTTCTTTCT CAGAGCTATG TTAAAATGTA TAACAAAGCA
    GTCAAACTGT GGGTCATTGC TCGAGAGCGG TTTCAGCAAG CTGCAGATCT GATTGATGCT
    GAGCAGAGAA TGAAGAAATC CATGTGGGGT CAGTTCTGGT CTGCTCACCA GAGATTTTTC
    AAATACTTGT GCATAGCGTC CAAAGTTAAA AGAGTTGTGC AACTAGCTCG AGAAGAAATC
    AAGAATGGAA AATGTGTTGT AATTGGTCTG CAGTCTACGG GAGAAGCTAG AACATTAGAA
    GCCTTGGAAG AGGGCGGGGG AGAATTGAAT GATTTTGTTT CAACTGCCAA AGGAGTGTTA
    CAGTCACTCA TTGAAAAACA CTTCCCTGCT CCGGATAGAA AAAAGCTTTA TAGTTTGCTG
    GGAATTGATT TGACAGCTCC AAGTAACAGC AGTTCACCAA GAGATAGTCC TTGTAAGGAA
    AATAAAACAA AGAAGCGGAA AGGTGAAGAA ATAACTCGAG AAGCCAAAAA AGCACGAAAA
    ATAGGTGGCC TTACTGGTAG CAGTTCTGAT GATAGTGGAA GTGAATCTGA TGCCTCTGAT
    AATGAAGAAA GTGACTATGA GAGCTCTAAA AACATGAGTT CTGGAGATGA TGATGATTTC
    AACCCATTCA GAGATGAGTC TAGTGAGGAT GATGAAGATG ATCCCTGGTT AATCAGAAAA
    GATCATAAGA AAAACAAAGA TAAAAAAAAA AGGAAAAGTA TAGATCCAGA TTCTATTCAA
    AGTGCCTTAT TAGCGTCAGG TCTTGGATCA AAACGACCTA GTTTTTCTTC TACACCAGTT
    ATTTCACCTG CTCCTAACAG TGCACCAGCT AACAGTAACA CCAACAGCAA CAGTAGCCTT
    ATAACAAGTC AAGATGCCGT GGAAAGGGCC CAACAGATGA AGAAAGACCT ACTTGATAAA
    CTAGAAAAAT TAGCTGAAGA CCTCCCTCCT AATACCCTGG ATGAACTTAT TGATGAACTT
    GGTGGCCCTG AAAATGTTGC TGAGATGACT GGCCGCAAGG GGAGGGTCGT AAGCAATGAT
    GACGGAAGCA TATCTTATGA GTCAAGATCT GAACTTGATG TTCCTGTGGA AATACTAAAT
    ATTACAGAAA AACAAAGATT TATGGATGGA GATAAGAATA TTGCTATCAT CTCAGAAGCT
    GCCAGCTCAG GTATTTCATT ACAAGCGGAT AGAAGGGCTA AAAATCAAAG GCGAAGAGTT
    CATATGACTC TGGAGTTACC ATGGAGTGCT GATAGAGCAA TTCAGCAATT TGGACGAACT
    CATAGATCAA ATCAAGTTAC CGCTCCTGAA TATGTCTTTC TGATTTCTGA ATTGGCAGGA
    GAACAAAGAT TTGCATCTAT TGTTGCTAAA AGACTTGAGA GTTTGGGCGC ACTTACACAT
    GGAGACAGAA GAGCAACAGA ATCTAGAGAT CTGAGCCGGT TCAACTTCGA TAATAAGTAT
    GGAAGAAATG CTTTAGAAAT TGTCATGAAA TCCATTGTAA ACCTAGATTC TCCTATGGTA
    TCACCACCTC CAGACTATCC TGGAGAATTC TTTAAAGATG TTCGACAAGG ACTGATAGGA
    GTTGGTCTAA TAAATGTAGA AGATCGTTCA GGAATTCTTA CTCTAGATAA AGATTATAAC
    AACATAGGAA AATTCTTAAA CAGAATTTTG GGCATGGAAG TGCATCAACA GAATGCACTA
    TTTCAGTATT TCGCAGACAC ACTTACTGCA GTCGTTCAAA ATGCCAAAAA AAATGGAAGA
    TACGATATGG GAATCTTAGA TCTTGGTTCT GGGGATGAAA AAGTGAGGAA AAGTGATGTT
    AAGAAGTTTC TGACTCCAGG ATATTCAACA TCTGGCCATG TAGAATTATA CACAATAAGT
    GTGGAGAGGG GAATGTCCTG GGAGGAAGCT ACCAAGATTT GGGCTGAGCT GACAGGACCA
    GATGATGGCT TTTATTTGTC ATTGCAAATA AGAAACAACA AGAAAACTGC CATCTTAGTT
    AAAGAAGTAA ACCCTAAAAA GAAGCTTTTC TTAGTTTATA GACCAAATAC CGGGAAACAA
    CTCAAATTAG AAATTTATGC TGATCTAAAA AAGAAATATA AGAAGGTAGT CTCAGACGAT
    GCCGTGGTCC ACTGGTTAGA TCAGTATAAT TCATCTGCAG ATACTTGTAC TCATGCTTAT
    TGGCGTGGCA ACTGCAAAAA AGCAAGCTTG GGATTAGTTT GTGAAATAGG TCTTCGTTGT
    CGCACATACT ATGTATTATG TGGCTCAGTA CTGAGTGTCT GGACAAAAGT TGAGGGTGTT
    CTTGCGTCTG TCAGTGGCAC AAATGTGAAA ATGCAGATAG TTCGCCTAAG AACAGAGGAT
    GGACAGCGGA TTGTAGGTTT GATCATTCCT GCAAATTGTG TGTCTCCTCT TATAAATCTC
    CTTTCAACTT CAGACCAGTC TCAACAGCTT GCAGTCCAGC AGAAACAGCT CTGGCAGCAG
    CGTCACCCCC AGAGCATTGC CAGCTTGAGC AGCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195081.1
    CDS136..4314
    Translation

    Target ORF information:

    RefSeq Version XM_005195024.3
    Organism Bos taurus(cattle)
    Definition Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195024.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGTCAGTGCC ACTTAGTGCA TTAGAACTAG GTTTGGAGAC TGAAGCAGCA
    GTTCCTGTTA AACAAGAACC AGAGACGGTA CCTACTCCAG CACTTTTAAA TGTTAGGCAG
    CCTCCATCTA CTACAACATT TGTGCTGAAT CAAATAAATC AGCTTCCAAC CTTGGGATCT
    ACAATTGTAA TGACTAAGAC ACCACCTGTA ACCACGAATA GGCAAACCAT CACTTTAACG
    AAGTTCATTC AGACTACTGC AAACACACGC CCTTCAGTCT CAGCACCAGC AGTACGAAAT
    GCCATGACTT CCGCACCTTC AAAAGACCAA GTTCAACTGA AGGACCTGCT AAAAAATAAT
    AGTCTGAATG AACTCATGAA ACTGAAGCCA CCTGCTAATA TTGCTCAGCC AGTTGCAACA
    GCAGCTACTG ATGTAAGCAA TGGCACAGTA AAGAAAGAGT CTTCTAATAA AGAAGTAGCA
    AGAATATGGA TAAATGACAT GAAAATGAGG AGTTTTTCCC CAACCATGAA GGTCCCTGTT
    GTGAAAGAGG AAGATGAACC AGAAGAAGAA GACGAAGAAG AAATGGGTCA TGCGGAAACC
    TATGCAGAGT ATATGCCAAT AAAATTAAAA ATTGGCCTAC GTCACCCAGA TGCCGTAGTT
    GAGACTAGTT CTTTATCCAG TGTTACTCCT CCTGATGTTT GGTACAAGAC ATCTATTTCT
    GAAGAAACCA TTGATAATGG CTGGTTATCA GCATTACAGC TTGAAGCAAT TACTTATGCA
    GCCCAGCAAC ATGAAACGTT CCTACCTAAT GGAGATCGTG CTGGCTTCTT AATAGGCGAT
    GGTGCTGGTG TAGGAAAAGG AAGGACAATA GCAGGAATCA TCTATGAAAA TTATTTGTTG
    AGTAGAAAAA GGGCTTTGTG GTTCAGTGTT TCAAATGATT TAAAGTATGA TGCTGAAAGA
    GATTTGAGGG ATATTGGAGC AAAAAACATT TTGGTCCATT CATTGAATAA GTTTAAATAT
    GGAAAAATTT CTTCCAAACA TAATGGGAGT GTGAAGAAGG GTGTTATTTT TGCTACCTAT
    TCTTCCCTTA TTGGTGAAAG TCAGTCTGGT GGTAAATATA AAACGAGGTT AAAACAACTT
    CTACATTGGT GCGGTGATGA CTTCGATGGA GTGATAGTTT TTGATGAATG TCATAAAGCA
    AAAAACTTAT GTCCTGTTGG TTCTTCAAAG CCAACCAAGA CAGGCTTAGC AGTTTTAGAA
    CTTCAGAACA AATTGCCAAA AGCTAGAGTT GTCTATGCTA GTGCCACTGG TGCTTCTGAA
    CCACGAAATA TGGCCTATAT GAACCGTCTT GGAATATGGG GTGAGGGAAC TCCATTTAGA
    GAATTCAGTG ACTTTATTCA GGCAGTAGAA CGGAGAGGTG TTGGTGCCAT GGAAATAGTT
    GCTATGGATA TGAAGCTTAG AGGAATGTAC ATTGCTCGAC AGCTGAGCTT TACTGGAGTG
    ACCTTCAAAA TTGAGGAAGT TCTTCTTTCT CAGAGCTATG TTAAAATGTA TAACAAAGCA
    GTCAAACTGT GGGTCATTGC TCGAGAGCGG TTTCAGCAAG CTGCAGATCT GATTGATGCT
    GAGCAGAGAA TGAAGAAATC CATGTGGGGT CAGTTCTGGT CTGCTCACCA GAGATTTTTC
    AAATACTTGT GCATAGCGTC CAAAGTTAAA AGAGTTGTGC AACTAGCTCG AGAAGAAATC
    AAGAATGGAA AATGTGTTGT AATTGGTCTG CAGTCTACGG GAGAAGCTAG AACATTAGAA
    GCCTTGGAAG AGGGCGGGGG AGAATTGAAT GATTTTGTTT CAACTGCCAA AGGAGTGTTA
    CAGTCACTCA TTGAAAAACA CTTCCCTGCT CCGGATAGAA AAAAGCTTTA TAGTTTGCTG
    GGAATTGATT TGACAGCTCC AAGTAACAGC AGTTCACCAA GAGATAGTCC TTGTAAGGAA
    AATAAAACAA AGAAGCGGAA AGGTGAAGAA ATAACTCGAG AAGCCAAAAA AGCACGAAAA
    ATAGGTGGCC TTACTGGTAG CAGTTCTGAT GATAGTGGAA GTGAATCTGA TGCCTCTGAT
    AATGAAGAAA GTGACTATGA GAGCTCTAAA AACATGAGTT CTGGAGATGA TGATGATTTC
    AACCCATTCA GAGATGAGTC TAGTGAGGAT GATGAAGATG ATCCCTGGTT AATCAGAAAA
    GATCATAAGA AAAACAAAGA TAAAAAAAAA AGGAAAAGTA TAGATCCAGA TTCTATTCAA
    AGTGCCTTAT TAGCGTCAGG TCTTGGATCA AAACGACCTA GTTTTTCTTC TACACCAGTT
    ATTTCACCTG CTCCTAACAG TGCACCAGCT AACAGTAACA CCAACAGCAA CAGTAGCCTT
    ATAACAAGTC AAGATGCCGT GGAAAGGGCC CAACAGATGA AGAAAGACCT ACTTGATAAA
    CTAGAAAAAT TAGCTGAAGA CCTCCCTCCT AATACCCTGG ATGAACTTAT TGATGAACTT
    GGTGGCCCTG AAAATGTTGC TGAGATGACT GGCCGCAAGG GGAGGGTCGT AAGCAATGAT
    GACGGAAGCA TATCTTATGA GTCAAGATCT GAACTTGATG TTCCTGTGGA AATACTAAAT
    ATTACAGAAA AACAAAGATT TATGGATGGA GATAAGAATA TTGCTATCAT CTCAGAAGCT
    GCCAGCTCAG GTATTTCATT ACAAGCGGAT AGAAGGGCTA AAAATCAAAG GCGAAGAGTT
    CATATGACTC TGGAGTTACC ATGGAGTGCT GATAGAGCAA TTCAGCAATT TGGACGAACT
    CATAGATCAA ATCAAGTTAC CGCTCCTGAA TATGTCTTTC TGATTTCTGA ATTGGCAGGA
    GAACAAAGAT TTGCATCTAT TGTTGCTAAA AGACTTGAGA GTTTGGGCGC ACTTACACAT
    GGAGACAGAA GAGCAACAGA ATCTAGAGAT CTGAGCCGGT TCAACTTCGA TAATAAGTAT
    GGAAGAAATG CTTTAGAAAT TGTCATGAAA TCCATTGTAA ACCTAGATTC TCCTATGGTA
    TCACCACCTC CAGACTATCC TGGAGAATTC TTTAAAGATG TTCGACAAGG ACTGATAGGA
    GTTGGTCTAA TAAATGTAGA AGATCGTTCA GGAATTCTTA CTCTAGATAA AGATTATAAC
    AACATAGGAA AATTCTTAAA CAGAATTTTG GGCATGGAAG TGCATCAACA GAATGCACTA
    TTTCAGTATT TCGCAGACAC ACTTACTGCA GTCGTTCAAA ATGCCAAAAA AAATGGAAGA
    TACGATATGG GAATCTTAGA TCTTGGTTCT GGGGATGAAA AAGTGAGGAA AAGTGATGTT
    AAGAAGTTTC TGACTCCAGG ATATTCAACA TCTGGCCATG TAGAATTATA CACAATAAGT
    GTGGAGAGGG GAATGTCCTG GGAGGAAGCT ACCAAGATTT GGGCTGAGCT GACAGGACCA
    GATGATGGCT TTTATTTGTC ATTGCAAATA AGAAACAACA AGAAAACTGC CATCTTAGTT
    AAAGAAGTAA ACCCTAAAAA GAAGCTTTTC TTAGTTTATA GACCAAATAC CGGGAAACAA
    CTCAAATTAG AAATTTATGC TGATCTAAAA AAGAAATATA AGAAGGTAGT CTCAGACGAT
    GCCGTGGTCC ACTGGTTAGA TCAGTATAAT TCATCTGCAG ATACTTGTAC TCATGCTTAT
    TGGCGTGGCA ACTGCAAAAA AGCAAGCTTG GGATTAGTTT GTGAAATAGG TCTTCGTTGT
    CGCACATACT ATGTATTATG TGGCTCAGTA CTGAGTGTCT GGACAAAAGT TGAGGGTGTT
    CTTGCGTCTG TCAGTGGCAC AAATGTGAAA ATGCAGATAG TTCGCCTAAG AACAGAGGAT
    GGACAGCGGA TTGTAGGTTT GATCATTCCT GCAAATTGTG TGTCTCCTCT TATAAATCTC
    CTTTCAACTT CAGACCAGTC TCAACAGCTT GCAGTCCAGC AGAAACAGCT CTGGCAGCAG
    CGTCACCCCC AGAGCATTGC CAGCTTGAGC AGCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54721
    Clone ID Related Accession (Same CDS sequence) XM_010823358.2 , XM_010814004.2 , XM_010814004.3
    Accession Version XM_010823358.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein strawberry notch homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGCAGCAGCC TCCATCTACT ACAACATTTG TGCTGAATCA AATAAATCAG
    CTTCCAACCT TGGGATCTAC AATTGTAATG ACTAAGACAC CACCTGTAAC CACGAATAGG
    CAAACCATCA CTTTAACGAA GTTCATTCAG ACTACTGCAA ACACACGCCC TTCAGTCTCA
    GCACCAGCAG TACGAAATGC CATGACTTCC GCACCTTCAA AAGACCAAGT TCAACTGAAG
    GACCTGCTAA AAAATAATAG TCTGAATGAA CTCATGAAAC TGAAGCCACC TGCTAATATT
    GCTCAGCCAG TTGCAACAGC AGCTACTGAT GTAAGCAATG GCACAGTAAA GAAAGAGTCT
    TCTAATAAAG AAGTAGCAAG AATATGGATA AATGACATGA AAATGAGGAG TTTTTCCCCA
    ACCATGAAGG TCCCTGTTGT GAAAGAGGAA GATGAACCAG AAGAAGAAGA CGAAGAAGAA
    ATGGGTCATG CGGAAACCTA TGCAGAGTAT ATGCCAATAA AATTAAAAAT TGGCCTACGT
    CACCCAGATG CCGTAGTTGA GACTAGTTCT TTATCCAGTG TTACTCCTCC TGATGTTTGG
    TACAAGACAT CTATTTCTGA AGAAACCATT GATAATGGCT GGTTATCAGC ATTACAGCTT
    GAAGCAATTA CTTATGCAGC CCAGCAACAT GAAACGTTCC TACCTAATGG AGATCGTGCT
    GGCTTCTTAA TAGGCGATGG TGCTGGTGTA GGAAAAGGAA GGACAATAGC AGGAATCATC
    TATGAAAATT ATTTGTTGAG TAGAAAAAGG GCTTTGTGGT TCAGTGTTTC AAATGATTTA
    AAGTATGATG CTGAAAGAGA TTTGAGGGAT ATTGGAGCAA AAAACATTTT GGTCCATTCA
    TTGAATAAGT TTAAATATGG AAAAATTTCT TCCAAACATA ATGGGAGTGT GAAGAAGGGT
    GTTATTTTTG CTACCTATTC TTCCCTTATT GGTGAAAGTC AGTCTGGTGG TAAATATAAA
    ACGAGGTTAA AACAACTTCT ACATTGGTGC GGTGATGACT TCGATGGAGT GATAGTTTTT
    GATGAATGTC ATAAAGCAAA AAACTTATGT CCTGTTGGTT CTTCAAAGCC AACCAAGACA
    GGCTTAGCAG TTTTAGAACT TCAGAACAAA TTGCCAAAAG CTAGAGTTGT CTATGCTAGT
    GCCACTGGTG CTTCTGAACC ACGAAATATG GCCTATATGA ACCGTCTTGG AATATGGGGT
    GAGGGAACTC CATTTAGAGA ATTCAGTGAC TTTATTCAGG CAGTAGAACG GAGAGGTGTT
    GGTGCCATGG AAATAGTTGC TATGGATATG AAGCTTAGAG GAATGTACAT TGCTCGACAG
    CTGAGCTTTA CTGGAGTGAC CTTCAAAATT GAGGAAGTTC TTCTTTCTCA GAGCTATGTT
    AAAATGTATA ACAAAGCAGT CAAACTGTGG GTCATTGCTC GAGAGCGGTT TCAGCAAGCT
    GCAGATCTGA TTGATGCTGA GCAGAGAATG AAGAAATCCA TGTGGGGTCA GTTCTGGTCT
    GCTCACCAGA GATTTTTCAA ATACTTGTGC ATAGCGTCCA AAGTTAAAAG AGTTGTGCAA
    CTAGCTCGAG AAGAAATCAA GAATGGAAAA TGTGTTGTAA TTGGTCTGCA GTCTACGGGA
    GAAGCTAGAA CATTAGAAGC CTTGGAAGAG GGCGGGGGAG AATTGAATGA TTTTGTTTCA
    ACTGCCAAAG GAGTGTTACA GTCACTCATT GAAAAACACT TCCCTGCTCC GGATAGAAAA
    AAGCTTTATA GTTTGCTGGG AATTGATTTG ACAGCTCCAA GTAACAGCAG TTCACCAAGA
    GATAGTCCTT GTAAGGAAAA TAAAACAAAG AAGCGGAAAG GTGAAGAAAT AACTCGAGAA
    GCCAAAAAAG CACGAAAAAT AGGTGGCCTT ACTGGTAGCA GTTCTGATGA TAGTGGAAGT
    GAATCTGATG CCTCTGATAA TGAAGAAAGT GACTATGAGA GCTCTAAAAA CATGAGTTCT
    GGAGATGATG ATGATTTCAA CCCATTCAGA GATGAGTCTA GTGAGGATGA TGAAGATGAT
    CCCTGGTTAA TCAGAAAAGA TCATAAGAAA AACAAAGATA AAAAAAAAAG GAAAAGTATA
    GATCCAGATT CTATTCAAAG TGCCTTATTA GCGTCAGGTC TTGGATCAAA ACGACCTAGT
    TTTTCTTCTA CACCAGTTAT TTCACCTGCT CCTAACAGTG CACCAGCTAA CAGTAACACC
    AACAGCAACA GTAGCCTTAT AACAAGTCAA GATGCCGTGG AAAGGGCCCA ACAGATGAAG
    AAAGACCTAC TTGATAAACT AGAAAAATTA GCTGAAGACC TCCCTCCTAA TACCCTGGAT
    GAACTTATTG ATGAACTTGG TGGCCCTGAA AATGTTGCTG AGATGACTGG CCGCAAGGGG
    AGGGTCGTAA GCAATGATGA CGGAAGCATA TCTTATGAGT CAAGATCTGA ACTTGATGTT
    CCTGTGGAAA TACTAAATAT TACAGAAAAA CAAAGATTTA TGGATGGAGA TAAGAATATT
    GCTATCATCT CAGAAGCTGC CAGCTCAGGT ATTTCATTAC AAGCGGATAG AAGGGCTAAA
    AATCAAAGGC GAAGAGTTCA TATGACTCTG GAGTTACCAT GGAGTGCTGA TAGAGCAATT
    CAGCAATTTG GACGAACTCA TAGATCAAAT CAAGTTACCG CTCCTGAATA TGTCTTTCTG
    ATTTCTGAAT TGGCAGGAGA ACAAAGATTT GCATCTATTG TTGCTAAAAG ACTTGAGAGT
    TTGGGCGCAC TTACACATGG AGACAGAAGA GCAACAGAAT CTAGAGATCT GAGCCGGTTC
    AACTTCGATA ATAAGTATGG AAGAAATGCT TTAGAAATTG TCATGAAATC CATTGTAAAC
    CTAGATTCTC CTATGGTATC ACCACCTCCA GACTATCCTG GAGAATTCTT TAAAGATGTT
    CGACAAGGAC TGATAGGAGT TGGTCTAATA AATGTAGAAG ATCGTTCAGG AATTCTTACT
    CTAGATAAAG ATTATAACAA CATAGGAAAA TTCTTAAACA GAATTTTGGG CATGGAAGTG
    CATCAACAGA ATGCACTATT TCAGTATTTC GCAGACACAC TTACTGCAGT CGTTCAAAAT
    GCCAAAAAAA ATGGAAGATA CGATATGGGA ATCTTAGATC TTGGTTCTGG GGATGAAAAA
    GTGAGGAAAA GTGATGTTAA GAAGTTTCTG ACTCCAGGAT ATTCAACATC TGGCCATGTA
    GAATTATACA CAATAAGTGT GGAGAGGGGA ATGTCCTGGG AGGAAGCTAC CAAGATTTGG
    GCTGAGCTGA CAGGACCAGA TGATGGCTTT TATTTGTCAT TGCAAATAAG AAACAACAAG
    AAAACTGCCA TCTTAGTTAA AGAAGTAAAC CCTAAAAAGA AGCTTTTCTT AGTTTATAGA
    CCAAATACCG GGAAACAACT CAAATTAGAA ATTTATGCTG ATCTAAAAAA GAAATATAAG
    AAGGTAGTCT CAGACGATGC CGTGGTCCAC TGGTTAGATC AGTATAATTC ATCTGCAGAT
    ACTTGTACTC ATGCTTATTG GCGTGGCAAC TGCAAAAAAG CAAGCTTGGG ATTAGTTTGT
    GAAATAGGTC TTCGTTGTCG CACATACTAT GTATTATGTG GCTCAGTACT GAGTGTCTGG
    ACAAAAGTTG AGGGTGTTCT TGCGTCTGTC AGTGGCACAA ATGTGAAAAT GCAGATAGTT
    CGCCTAAGAA CAGAGGATGG ACAGCGGATT GTAGGTTTGA TCATTCCTGC AAATTGTGTG
    TCTCCTCTTA TAAATCTCCT TTCAACTTCA GACCAGTCTC AACAGCTTGC AGTCCAGCAG
    AAACAGCTCT GGCAGCAGCG TCACCCCCAG AGCATTGCCA GCTTGAGCAG CACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821660.1
    CDS136..4212
    Translation

    Target ORF information:

    RefSeq Version XM_010823358.2
    Organism Bos taurus(cattle)
    Definition Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823358.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGCAGCAGCC TCCATCTACT ACAACATTTG TGCTGAATCA AATAAATCAG
    CTTCCAACCT TGGGATCTAC AATTGTAATG ACTAAGACAC CACCTGTAAC CACGAATAGG
    CAAACCATCA CTTTAACGAA GTTCATTCAG ACTACTGCAA ACACACGCCC TTCAGTCTCA
    GCACCAGCAG TACGAAATGC CATGACTTCC GCACCTTCAA AAGACCAAGT TCAACTGAAG
    GACCTGCTAA AAAATAATAG TCTGAATGAA CTCATGAAAC TGAAGCCACC TGCTAATATT
    GCTCAGCCAG TTGCAACAGC AGCTACTGAT GTAAGCAATG GCACAGTAAA GAAAGAGTCT
    TCTAATAAAG AAGTAGCAAG AATATGGATA AATGACATGA AAATGAGGAG TTTTTCCCCA
    ACCATGAAGG TCCCTGTTGT GAAAGAGGAA GATGAACCAG AAGAAGAAGA CGAAGAAGAA
    ATGGGTCATG CGGAAACCTA TGCAGAGTAT ATGCCAATAA AATTAAAAAT TGGCCTACGT
    CACCCAGATG CCGTAGTTGA GACTAGTTCT TTATCCAGTG TTACTCCTCC TGATGTTTGG
    TACAAGACAT CTATTTCTGA AGAAACCATT GATAATGGCT GGTTATCAGC ATTACAGCTT
    GAAGCAATTA CTTATGCAGC CCAGCAACAT GAAACGTTCC TACCTAATGG AGATCGTGCT
    GGCTTCTTAA TAGGCGATGG TGCTGGTGTA GGAAAAGGAA GGACAATAGC AGGAATCATC
    TATGAAAATT ATTTGTTGAG TAGAAAAAGG GCTTTGTGGT TCAGTGTTTC AAATGATTTA
    AAGTATGATG CTGAAAGAGA TTTGAGGGAT ATTGGAGCAA AAAACATTTT GGTCCATTCA
    TTGAATAAGT TTAAATATGG AAAAATTTCT TCCAAACATA ATGGGAGTGT GAAGAAGGGT
    GTTATTTTTG CTACCTATTC TTCCCTTATT GGTGAAAGTC AGTCTGGTGG TAAATATAAA
    ACGAGGTTAA AACAACTTCT ACATTGGTGC GGTGATGACT TCGATGGAGT GATAGTTTTT
    GATGAATGTC ATAAAGCAAA AAACTTATGT CCTGTTGGTT CTTCAAAGCC AACCAAGACA
    GGCTTAGCAG TTTTAGAACT TCAGAACAAA TTGCCAAAAG CTAGAGTTGT CTATGCTAGT
    GCCACTGGTG CTTCTGAACC ACGAAATATG GCCTATATGA ACCGTCTTGG AATATGGGGT
    GAGGGAACTC CATTTAGAGA ATTCAGTGAC TTTATTCAGG CAGTAGAACG GAGAGGTGTT
    GGTGCCATGG AAATAGTTGC TATGGATATG AAGCTTAGAG GAATGTACAT TGCTCGACAG
    CTGAGCTTTA CTGGAGTGAC CTTCAAAATT GAGGAAGTTC TTCTTTCTCA GAGCTATGTT
    AAAATGTATA ACAAAGCAGT CAAACTGTGG GTCATTGCTC GAGAGCGGTT TCAGCAAGCT
    GCAGATCTGA TTGATGCTGA GCAGAGAATG AAGAAATCCA TGTGGGGTCA GTTCTGGTCT
    GCTCACCAGA GATTTTTCAA ATACTTGTGC ATAGCGTCCA AAGTTAAAAG AGTTGTGCAA
    CTAGCTCGAG AAGAAATCAA GAATGGAAAA TGTGTTGTAA TTGGTCTGCA GTCTACGGGA
    GAAGCTAGAA CATTAGAAGC CTTGGAAGAG GGCGGGGGAG AATTGAATGA TTTTGTTTCA
    ACTGCCAAAG GAGTGTTACA GTCACTCATT GAAAAACACT TCCCTGCTCC GGATAGAAAA
    AAGCTTTATA GTTTGCTGGG AATTGATTTG ACAGCTCCAA GTAACAGCAG TTCACCAAGA
    GATAGTCCTT GTAAGGAAAA TAAAACAAAG AAGCGGAAAG GTGAAGAAAT AACTCGAGAA
    GCCAAAAAAG CACGAAAAAT AGGTGGCCTT ACTGGTAGCA GTTCTGATGA TAGTGGAAGT
    GAATCTGATG CCTCTGATAA TGAAGAAAGT GACTATGAGA GCTCTAAAAA CATGAGTTCT
    GGAGATGATG ATGATTTCAA CCCATTCAGA GATGAGTCTA GTGAGGATGA TGAAGATGAT
    CCCTGGTTAA TCAGAAAAGA TCATAAGAAA AACAAAGATA AAAAAAAAAG GAAAAGTATA
    GATCCAGATT CTATTCAAAG TGCCTTATTA GCGTCAGGTC TTGGATCAAA ACGACCTAGT
    TTTTCTTCTA CACCAGTTAT TTCACCTGCT CCTAACAGTG CACCAGCTAA CAGTAACACC
    AACAGCAACA GTAGCCTTAT AACAAGTCAA GATGCCGTGG AAAGGGCCCA ACAGATGAAG
    AAAGACCTAC TTGATAAACT AGAAAAATTA GCTGAAGACC TCCCTCCTAA TACCCTGGAT
    GAACTTATTG ATGAACTTGG TGGCCCTGAA AATGTTGCTG AGATGACTGG CCGCAAGGGG
    AGGGTCGTAA GCAATGATGA CGGAAGCATA TCTTATGAGT CAAGATCTGA ACTTGATGTT
    CCTGTGGAAA TACTAAATAT TACAGAAAAA CAAAGATTTA TGGATGGAGA TAAGAATATT
    GCTATCATCT CAGAAGCTGC CAGCTCAGGT ATTTCATTAC AAGCGGATAG AAGGGCTAAA
    AATCAAAGGC GAAGAGTTCA TATGACTCTG GAGTTACCAT GGAGTGCTGA TAGAGCAATT
    CAGCAATTTG GACGAACTCA TAGATCAAAT CAAGTTACCG CTCCTGAATA TGTCTTTCTG
    ATTTCTGAAT TGGCAGGAGA ACAAAGATTT GCATCTATTG TTGCTAAAAG ACTTGAGAGT
    TTGGGCGCAC TTACACATGG AGACAGAAGA GCAACAGAAT CTAGAGATCT GAGCCGGTTC
    AACTTCGATA ATAAGTATGG AAGAAATGCT TTAGAAATTG TCATGAAATC CATTGTAAAC
    CTAGATTCTC CTATGGTATC ACCACCTCCA GACTATCCTG GAGAATTCTT TAAAGATGTT
    CGACAAGGAC TGATAGGAGT TGGTCTAATA AATGTAGAAG ATCGTTCAGG AATTCTTACT
    CTAGATAAAG ATTATAACAA CATAGGAAAA TTCTTAAACA GAATTTTGGG CATGGAAGTG
    CATCAACAGA ATGCACTATT TCAGTATTTC GCAGACACAC TTACTGCAGT CGTTCAAAAT
    GCCAAAAAAA ATGGAAGATA CGATATGGGA ATCTTAGATC TTGGTTCTGG GGATGAAAAA
    GTGAGGAAAA GTGATGTTAA GAAGTTTCTG ACTCCAGGAT ATTCAACATC TGGCCATGTA
    GAATTATACA CAATAAGTGT GGAGAGGGGA ATGTCCTGGG AGGAAGCTAC CAAGATTTGG
    GCTGAGCTGA CAGGACCAGA TGATGGCTTT TATTTGTCAT TGCAAATAAG AAACAACAAG
    AAAACTGCCA TCTTAGTTAA AGAAGTAAAC CCTAAAAAGA AGCTTTTCTT AGTTTATAGA
    CCAAATACCG GGAAACAACT CAAATTAGAA ATTTATGCTG ATCTAAAAAA GAAATATAAG
    AAGGTAGTCT CAGACGATGC CGTGGTCCAC TGGTTAGATC AGTATAATTC ATCTGCAGAT
    ACTTGTACTC ATGCTTATTG GCGTGGCAAC TGCAAAAAAG CAAGCTTGGG ATTAGTTTGT
    GAAATAGGTC TTCGTTGTCG CACATACTAT GTATTATGTG GCTCAGTACT GAGTGTCTGG
    ACAAAAGTTG AGGGTGTTCT TGCGTCTGTC AGTGGCACAA ATGTGAAAAT GCAGATAGTT
    CGCCTAAGAA CAGAGGATGG ACAGCGGATT GTAGGTTTGA TCATTCCTGC AAATTGTGTG
    TCTCCTCTTA TAAATCTCCT TTCAACTTCA GACCAGTCTC AACAGCTTGC AGTCCAGCAG
    AAACAGCTCT GGCAGCAGCG TCACCCCCAG AGCATTGCCA GCTTGAGCAG CACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54721
    Clone ID Related Accession (Same CDS sequence) XM_010823358.2 , XM_010814004.2 , XM_010814004.3
    Accession Version XM_010814004.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein strawberry notch homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGCAGCAGCC TCCATCTACT ACAACATTTG TGCTGAATCA AATAAATCAG
    CTTCCAACCT TGGGATCTAC AATTGTAATG ACTAAGACAC CACCTGTAAC CACGAATAGG
    CAAACCATCA CTTTAACGAA GTTCATTCAG ACTACTGCAA ACACACGCCC TTCAGTCTCA
    GCACCAGCAG TACGAAATGC CATGACTTCC GCACCTTCAA AAGACCAAGT TCAACTGAAG
    GACCTGCTAA AAAATAATAG TCTGAATGAA CTCATGAAAC TGAAGCCACC TGCTAATATT
    GCTCAGCCAG TTGCAACAGC AGCTACTGAT GTAAGCAATG GCACAGTAAA GAAAGAGTCT
    TCTAATAAAG AAGTAGCAAG AATATGGATA AATGACATGA AAATGAGGAG TTTTTCCCCA
    ACCATGAAGG TCCCTGTTGT GAAAGAGGAA GATGAACCAG AAGAAGAAGA CGAAGAAGAA
    ATGGGTCATG CGGAAACCTA TGCAGAGTAT ATGCCAATAA AATTAAAAAT TGGCCTACGT
    CACCCAGATG CCGTAGTTGA GACTAGTTCT TTATCCAGTG TTACTCCTCC TGATGTTTGG
    TACAAGACAT CTATTTCTGA AGAAACCATT GATAATGGCT GGTTATCAGC ATTACAGCTT
    GAAGCAATTA CTTATGCAGC CCAGCAACAT GAAACGTTCC TACCTAATGG AGATCGTGCT
    GGCTTCTTAA TAGGCGATGG TGCTGGTGTA GGAAAAGGAA GGACAATAGC AGGAATCATC
    TATGAAAATT ATTTGTTGAG TAGAAAAAGG GCTTTGTGGT TCAGTGTTTC AAATGATTTA
    AAGTATGATG CTGAAAGAGA TTTGAGGGAT ATTGGAGCAA AAAACATTTT GGTCCATTCA
    TTGAATAAGT TTAAATATGG AAAAATTTCT TCCAAACATA ATGGGAGTGT GAAGAAGGGT
    GTTATTTTTG CTACCTATTC TTCCCTTATT GGTGAAAGTC AGTCTGGTGG TAAATATAAA
    ACGAGGTTAA AACAACTTCT ACATTGGTGC GGTGATGACT TCGATGGAGT GATAGTTTTT
    GATGAATGTC ATAAAGCAAA AAACTTATGT CCTGTTGGTT CTTCAAAGCC AACCAAGACA
    GGCTTAGCAG TTTTAGAACT TCAGAACAAA TTGCCAAAAG CTAGAGTTGT CTATGCTAGT
    GCCACTGGTG CTTCTGAACC ACGAAATATG GCCTATATGA ACCGTCTTGG AATATGGGGT
    GAGGGAACTC CATTTAGAGA ATTCAGTGAC TTTATTCAGG CAGTAGAACG GAGAGGTGTT
    GGTGCCATGG AAATAGTTGC TATGGATATG AAGCTTAGAG GAATGTACAT TGCTCGACAG
    CTGAGCTTTA CTGGAGTGAC CTTCAAAATT GAGGAAGTTC TTCTTTCTCA GAGCTATGTT
    AAAATGTATA ACAAAGCAGT CAAACTGTGG GTCATTGCTC GAGAGCGGTT TCAGCAAGCT
    GCAGATCTGA TTGATGCTGA GCAGAGAATG AAGAAATCCA TGTGGGGTCA GTTCTGGTCT
    GCTCACCAGA GATTTTTCAA ATACTTGTGC ATAGCGTCCA AAGTTAAAAG AGTTGTGCAA
    CTAGCTCGAG AAGAAATCAA GAATGGAAAA TGTGTTGTAA TTGGTCTGCA GTCTACGGGA
    GAAGCTAGAA CATTAGAAGC CTTGGAAGAG GGCGGGGGAG AATTGAATGA TTTTGTTTCA
    ACTGCCAAAG GAGTGTTACA GTCACTCATT GAAAAACACT TCCCTGCTCC GGATAGAAAA
    AAGCTTTATA GTTTGCTGGG AATTGATTTG ACAGCTCCAA GTAACAGCAG TTCACCAAGA
    GATAGTCCTT GTAAGGAAAA TAAAACAAAG AAGCGGAAAG GTGAAGAAAT AACTCGAGAA
    GCCAAAAAAG CACGAAAAAT AGGTGGCCTT ACTGGTAGCA GTTCTGATGA TAGTGGAAGT
    GAATCTGATG CCTCTGATAA TGAAGAAAGT GACTATGAGA GCTCTAAAAA CATGAGTTCT
    GGAGATGATG ATGATTTCAA CCCATTCAGA GATGAGTCTA GTGAGGATGA TGAAGATGAT
    CCCTGGTTAA TCAGAAAAGA TCATAAGAAA AACAAAGATA AAAAAAAAAG GAAAAGTATA
    GATCCAGATT CTATTCAAAG TGCCTTATTA GCGTCAGGTC TTGGATCAAA ACGACCTAGT
    TTTTCTTCTA CACCAGTTAT TTCACCTGCT CCTAACAGTG CACCAGCTAA CAGTAACACC
    AACAGCAACA GTAGCCTTAT AACAAGTCAA GATGCCGTGG AAAGGGCCCA ACAGATGAAG
    AAAGACCTAC TTGATAAACT AGAAAAATTA GCTGAAGACC TCCCTCCTAA TACCCTGGAT
    GAACTTATTG ATGAACTTGG TGGCCCTGAA AATGTTGCTG AGATGACTGG CCGCAAGGGG
    AGGGTCGTAA GCAATGATGA CGGAAGCATA TCTTATGAGT CAAGATCTGA ACTTGATGTT
    CCTGTGGAAA TACTAAATAT TACAGAAAAA CAAAGATTTA TGGATGGAGA TAAGAATATT
    GCTATCATCT CAGAAGCTGC CAGCTCAGGT ATTTCATTAC AAGCGGATAG AAGGGCTAAA
    AATCAAAGGC GAAGAGTTCA TATGACTCTG GAGTTACCAT GGAGTGCTGA TAGAGCAATT
    CAGCAATTTG GACGAACTCA TAGATCAAAT CAAGTTACCG CTCCTGAATA TGTCTTTCTG
    ATTTCTGAAT TGGCAGGAGA ACAAAGATTT GCATCTATTG TTGCTAAAAG ACTTGAGAGT
    TTGGGCGCAC TTACACATGG AGACAGAAGA GCAACAGAAT CTAGAGATCT GAGCCGGTTC
    AACTTCGATA ATAAGTATGG AAGAAATGCT TTAGAAATTG TCATGAAATC CATTGTAAAC
    CTAGATTCTC CTATGGTATC ACCACCTCCA GACTATCCTG GAGAATTCTT TAAAGATGTT
    CGACAAGGAC TGATAGGAGT TGGTCTAATA AATGTAGAAG ATCGTTCAGG AATTCTTACT
    CTAGATAAAG ATTATAACAA CATAGGAAAA TTCTTAAACA GAATTTTGGG CATGGAAGTG
    CATCAACAGA ATGCACTATT TCAGTATTTC GCAGACACAC TTACTGCAGT CGTTCAAAAT
    GCCAAAAAAA ATGGAAGATA CGATATGGGA ATCTTAGATC TTGGTTCTGG GGATGAAAAA
    GTGAGGAAAA GTGATGTTAA GAAGTTTCTG ACTCCAGGAT ATTCAACATC TGGCCATGTA
    GAATTATACA CAATAAGTGT GGAGAGGGGA ATGTCCTGGG AGGAAGCTAC CAAGATTTGG
    GCTGAGCTGA CAGGACCAGA TGATGGCTTT TATTTGTCAT TGCAAATAAG AAACAACAAG
    AAAACTGCCA TCTTAGTTAA AGAAGTAAAC CCTAAAAAGA AGCTTTTCTT AGTTTATAGA
    CCAAATACCG GGAAACAACT CAAATTAGAA ATTTATGCTG ATCTAAAAAA GAAATATAAG
    AAGGTAGTCT CAGACGATGC CGTGGTCCAC TGGTTAGATC AGTATAATTC ATCTGCAGAT
    ACTTGTACTC ATGCTTATTG GCGTGGCAAC TGCAAAAAAG CAAGCTTGGG ATTAGTTTGT
    GAAATAGGTC TTCGTTGTCG CACATACTAT GTATTATGTG GCTCAGTACT GAGTGTCTGG
    ACAAAAGTTG AGGGTGTTCT TGCGTCTGTC AGTGGCACAA ATGTGAAAAT GCAGATAGTT
    CGCCTAAGAA CAGAGGATGG ACAGCGGATT GTAGGTTTGA TCATTCCTGC AAATTGTGTG
    TCTCCTCTTA TAAATCTCCT TTCAACTTCA GACCAGTCTC AACAGCTTGC AGTCCAGCAG
    AAACAGCTCT GGCAGCAGCG TCACCCCCAG AGCATTGCCA GCTTGAGCAG CACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812306.1
    CDS136..4212
    Translation

    Target ORF information:

    RefSeq Version XM_010814004.2
    Organism Bos taurus(cattle)
    Definition Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814004.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGCAGCAGCC TCCATCTACT ACAACATTTG TGCTGAATCA AATAAATCAG
    CTTCCAACCT TGGGATCTAC AATTGTAATG ACTAAGACAC CACCTGTAAC CACGAATAGG
    CAAACCATCA CTTTAACGAA GTTCATTCAG ACTACTGCAA ACACACGCCC TTCAGTCTCA
    GCACCAGCAG TACGAAATGC CATGACTTCC GCACCTTCAA AAGACCAAGT TCAACTGAAG
    GACCTGCTAA AAAATAATAG TCTGAATGAA CTCATGAAAC TGAAGCCACC TGCTAATATT
    GCTCAGCCAG TTGCAACAGC AGCTACTGAT GTAAGCAATG GCACAGTAAA GAAAGAGTCT
    TCTAATAAAG AAGTAGCAAG AATATGGATA AATGACATGA AAATGAGGAG TTTTTCCCCA
    ACCATGAAGG TCCCTGTTGT GAAAGAGGAA GATGAACCAG AAGAAGAAGA CGAAGAAGAA
    ATGGGTCATG CGGAAACCTA TGCAGAGTAT ATGCCAATAA AATTAAAAAT TGGCCTACGT
    CACCCAGATG CCGTAGTTGA GACTAGTTCT TTATCCAGTG TTACTCCTCC TGATGTTTGG
    TACAAGACAT CTATTTCTGA AGAAACCATT GATAATGGCT GGTTATCAGC ATTACAGCTT
    GAAGCAATTA CTTATGCAGC CCAGCAACAT GAAACGTTCC TACCTAATGG AGATCGTGCT
    GGCTTCTTAA TAGGCGATGG TGCTGGTGTA GGAAAAGGAA GGACAATAGC AGGAATCATC
    TATGAAAATT ATTTGTTGAG TAGAAAAAGG GCTTTGTGGT TCAGTGTTTC AAATGATTTA
    AAGTATGATG CTGAAAGAGA TTTGAGGGAT ATTGGAGCAA AAAACATTTT GGTCCATTCA
    TTGAATAAGT TTAAATATGG AAAAATTTCT TCCAAACATA ATGGGAGTGT GAAGAAGGGT
    GTTATTTTTG CTACCTATTC TTCCCTTATT GGTGAAAGTC AGTCTGGTGG TAAATATAAA
    ACGAGGTTAA AACAACTTCT ACATTGGTGC GGTGATGACT TCGATGGAGT GATAGTTTTT
    GATGAATGTC ATAAAGCAAA AAACTTATGT CCTGTTGGTT CTTCAAAGCC AACCAAGACA
    GGCTTAGCAG TTTTAGAACT TCAGAACAAA TTGCCAAAAG CTAGAGTTGT CTATGCTAGT
    GCCACTGGTG CTTCTGAACC ACGAAATATG GCCTATATGA ACCGTCTTGG AATATGGGGT
    GAGGGAACTC CATTTAGAGA ATTCAGTGAC TTTATTCAGG CAGTAGAACG GAGAGGTGTT
    GGTGCCATGG AAATAGTTGC TATGGATATG AAGCTTAGAG GAATGTACAT TGCTCGACAG
    CTGAGCTTTA CTGGAGTGAC CTTCAAAATT GAGGAAGTTC TTCTTTCTCA GAGCTATGTT
    AAAATGTATA ACAAAGCAGT CAAACTGTGG GTCATTGCTC GAGAGCGGTT TCAGCAAGCT
    GCAGATCTGA TTGATGCTGA GCAGAGAATG AAGAAATCCA TGTGGGGTCA GTTCTGGTCT
    GCTCACCAGA GATTTTTCAA ATACTTGTGC ATAGCGTCCA AAGTTAAAAG AGTTGTGCAA
    CTAGCTCGAG AAGAAATCAA GAATGGAAAA TGTGTTGTAA TTGGTCTGCA GTCTACGGGA
    GAAGCTAGAA CATTAGAAGC CTTGGAAGAG GGCGGGGGAG AATTGAATGA TTTTGTTTCA
    ACTGCCAAAG GAGTGTTACA GTCACTCATT GAAAAACACT TCCCTGCTCC GGATAGAAAA
    AAGCTTTATA GTTTGCTGGG AATTGATTTG ACAGCTCCAA GTAACAGCAG TTCACCAAGA
    GATAGTCCTT GTAAGGAAAA TAAAACAAAG AAGCGGAAAG GTGAAGAAAT AACTCGAGAA
    GCCAAAAAAG CACGAAAAAT AGGTGGCCTT ACTGGTAGCA GTTCTGATGA TAGTGGAAGT
    GAATCTGATG CCTCTGATAA TGAAGAAAGT GACTATGAGA GCTCTAAAAA CATGAGTTCT
    GGAGATGATG ATGATTTCAA CCCATTCAGA GATGAGTCTA GTGAGGATGA TGAAGATGAT
    CCCTGGTTAA TCAGAAAAGA TCATAAGAAA AACAAAGATA AAAAAAAAAG GAAAAGTATA
    GATCCAGATT CTATTCAAAG TGCCTTATTA GCGTCAGGTC TTGGATCAAA ACGACCTAGT
    TTTTCTTCTA CACCAGTTAT TTCACCTGCT CCTAACAGTG CACCAGCTAA CAGTAACACC
    AACAGCAACA GTAGCCTTAT AACAAGTCAA GATGCCGTGG AAAGGGCCCA ACAGATGAAG
    AAAGACCTAC TTGATAAACT AGAAAAATTA GCTGAAGACC TCCCTCCTAA TACCCTGGAT
    GAACTTATTG ATGAACTTGG TGGCCCTGAA AATGTTGCTG AGATGACTGG CCGCAAGGGG
    AGGGTCGTAA GCAATGATGA CGGAAGCATA TCTTATGAGT CAAGATCTGA ACTTGATGTT
    CCTGTGGAAA TACTAAATAT TACAGAAAAA CAAAGATTTA TGGATGGAGA TAAGAATATT
    GCTATCATCT CAGAAGCTGC CAGCTCAGGT ATTTCATTAC AAGCGGATAG AAGGGCTAAA
    AATCAAAGGC GAAGAGTTCA TATGACTCTG GAGTTACCAT GGAGTGCTGA TAGAGCAATT
    CAGCAATTTG GACGAACTCA TAGATCAAAT CAAGTTACCG CTCCTGAATA TGTCTTTCTG
    ATTTCTGAAT TGGCAGGAGA ACAAAGATTT GCATCTATTG TTGCTAAAAG ACTTGAGAGT
    TTGGGCGCAC TTACACATGG AGACAGAAGA GCAACAGAAT CTAGAGATCT GAGCCGGTTC
    AACTTCGATA ATAAGTATGG AAGAAATGCT TTAGAAATTG TCATGAAATC CATTGTAAAC
    CTAGATTCTC CTATGGTATC ACCACCTCCA GACTATCCTG GAGAATTCTT TAAAGATGTT
    CGACAAGGAC TGATAGGAGT TGGTCTAATA AATGTAGAAG ATCGTTCAGG AATTCTTACT
    CTAGATAAAG ATTATAACAA CATAGGAAAA TTCTTAAACA GAATTTTGGG CATGGAAGTG
    CATCAACAGA ATGCACTATT TCAGTATTTC GCAGACACAC TTACTGCAGT CGTTCAAAAT
    GCCAAAAAAA ATGGAAGATA CGATATGGGA ATCTTAGATC TTGGTTCTGG GGATGAAAAA
    GTGAGGAAAA GTGATGTTAA GAAGTTTCTG ACTCCAGGAT ATTCAACATC TGGCCATGTA
    GAATTATACA CAATAAGTGT GGAGAGGGGA ATGTCCTGGG AGGAAGCTAC CAAGATTTGG
    GCTGAGCTGA CAGGACCAGA TGATGGCTTT TATTTGTCAT TGCAAATAAG AAACAACAAG
    AAAACTGCCA TCTTAGTTAA AGAAGTAAAC CCTAAAAAGA AGCTTTTCTT AGTTTATAGA
    CCAAATACCG GGAAACAACT CAAATTAGAA ATTTATGCTG ATCTAAAAAA GAAATATAAG
    AAGGTAGTCT CAGACGATGC CGTGGTCCAC TGGTTAGATC AGTATAATTC ATCTGCAGAT
    ACTTGTACTC ATGCTTATTG GCGTGGCAAC TGCAAAAAAG CAAGCTTGGG ATTAGTTTGT
    GAAATAGGTC TTCGTTGTCG CACATACTAT GTATTATGTG GCTCAGTACT GAGTGTCTGG
    ACAAAAGTTG AGGGTGTTCT TGCGTCTGTC AGTGGCACAA ATGTGAAAAT GCAGATAGTT
    CGCCTAAGAA CAGAGGATGG ACAGCGGATT GTAGGTTTGA TCATTCCTGC AAATTGTGTG
    TCTCCTCTTA TAAATCTCCT TTCAACTTCA GACCAGTCTC AACAGCTTGC AGTCCAGCAG
    AAACAGCTCT GGCAGCAGCG TCACCCCCAG AGCATTGCCA GCTTGAGCAG CACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54721
    Clone ID Related Accession (Same CDS sequence) XM_010823358.2 , XM_010814004.2 , XM_010814004.3
    Accession Version XM_010814004.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein strawberry notch homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814004.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGCAGCAGCC TCCATCTACT ACAACATTTG TGCTGAATCA AATAAATCAG
    CTTCCAACCT TGGGATCTAC AATTGTAATG ACTAAGACAC CACCTGTAAC CACGAATAGG
    CAAACCATCA CTTTAACGAA GTTCATTCAG ACTACTGCAA ACACACGCCC TTCAGTCTCA
    GCACCAGCAG TACGAAATGC CATGACTTCC GCACCTTCAA AAGACCAAGT TCAACTGAAG
    GACCTGCTAA AAAATAATAG TCTGAATGAA CTCATGAAAC TGAAGCCACC TGCTAATATT
    GCTCAGCCAG TTGCAACAGC AGCTACTGAT GTAAGCAATG GCACAGTAAA GAAAGAGTCT
    TCTAATAAAG AAGTAGCAAG AATATGGATA AATGACATGA AAATGAGGAG TTTTTCCCCA
    ACCATGAAGG TCCCTGTTGT GAAAGAGGAA GATGAACCAG AAGAAGAAGA CGAAGAAGAA
    ATGGGTCATG CGGAAACCTA TGCAGAGTAT ATGCCAATAA AATTAAAAAT TGGCCTACGT
    CACCCAGATG CCGTAGTTGA GACTAGTTCT TTATCCAGTG TTACTCCTCC TGATGTTTGG
    TACAAGACAT CTATTTCTGA AGAAACCATT GATAATGGCT GGTTATCAGC ATTACAGCTT
    GAAGCAATTA CTTATGCAGC CCAGCAACAT GAAACGTTCC TACCTAATGG AGATCGTGCT
    GGCTTCTTAA TAGGCGATGG TGCTGGTGTA GGAAAAGGAA GGACAATAGC AGGAATCATC
    TATGAAAATT ATTTGTTGAG TAGAAAAAGG GCTTTGTGGT TCAGTGTTTC AAATGATTTA
    AAGTATGATG CTGAAAGAGA TTTGAGGGAT ATTGGAGCAA AAAACATTTT GGTCCATTCA
    TTGAATAAGT TTAAATATGG AAAAATTTCT TCCAAACATA ATGGGAGTGT GAAGAAGGGT
    GTTATTTTTG CTACCTATTC TTCCCTTATT GGTGAAAGTC AGTCTGGTGG TAAATATAAA
    ACGAGGTTAA AACAACTTCT ACATTGGTGC GGTGATGACT TCGATGGAGT GATAGTTTTT
    GATGAATGTC ATAAAGCAAA AAACTTATGT CCTGTTGGTT CTTCAAAGCC AACCAAGACA
    GGCTTAGCAG TTTTAGAACT TCAGAACAAA TTGCCAAAAG CTAGAGTTGT CTATGCTAGT
    GCCACTGGTG CTTCTGAACC ACGAAATATG GCCTATATGA ACCGTCTTGG AATATGGGGT
    GAGGGAACTC CATTTAGAGA ATTCAGTGAC TTTATTCAGG CAGTAGAACG GAGAGGTGTT
    GGTGCCATGG AAATAGTTGC TATGGATATG AAGCTTAGAG GAATGTACAT TGCTCGACAG
    CTGAGCTTTA CTGGAGTGAC CTTCAAAATT GAGGAAGTTC TTCTTTCTCA GAGCTATGTT
    AAAATGTATA ACAAAGCAGT CAAACTGTGG GTCATTGCTC GAGAGCGGTT TCAGCAAGCT
    GCAGATCTGA TTGATGCTGA GCAGAGAATG AAGAAATCCA TGTGGGGTCA GTTCTGGTCT
    GCTCACCAGA GATTTTTCAA ATACTTGTGC ATAGCGTCCA AAGTTAAAAG AGTTGTGCAA
    CTAGCTCGAG AAGAAATCAA GAATGGAAAA TGTGTTGTAA TTGGTCTGCA GTCTACGGGA
    GAAGCTAGAA CATTAGAAGC CTTGGAAGAG GGCGGGGGAG AATTGAATGA TTTTGTTTCA
    ACTGCCAAAG GAGTGTTACA GTCACTCATT GAAAAACACT TCCCTGCTCC GGATAGAAAA
    AAGCTTTATA GTTTGCTGGG AATTGATTTG ACAGCTCCAA GTAACAGCAG TTCACCAAGA
    GATAGTCCTT GTAAGGAAAA TAAAACAAAG AAGCGGAAAG GTGAAGAAAT AACTCGAGAA
    GCCAAAAAAG CACGAAAAAT AGGTGGCCTT ACTGGTAGCA GTTCTGATGA TAGTGGAAGT
    GAATCTGATG CCTCTGATAA TGAAGAAAGT GACTATGAGA GCTCTAAAAA CATGAGTTCT
    GGAGATGATG ATGATTTCAA CCCATTCAGA GATGAGTCTA GTGAGGATGA TGAAGATGAT
    CCCTGGTTAA TCAGAAAAGA TCATAAGAAA AACAAAGATA AAAAAAAAAG GAAAAGTATA
    GATCCAGATT CTATTCAAAG TGCCTTATTA GCGTCAGGTC TTGGATCAAA ACGACCTAGT
    TTTTCTTCTA CACCAGTTAT TTCACCTGCT CCTAACAGTG CACCAGCTAA CAGTAACACC
    AACAGCAACA GTAGCCTTAT AACAAGTCAA GATGCCGTGG AAAGGGCCCA ACAGATGAAG
    AAAGACCTAC TTGATAAACT AGAAAAATTA GCTGAAGACC TCCCTCCTAA TACCCTGGAT
    GAACTTATTG ATGAACTTGG TGGCCCTGAA AATGTTGCTG AGATGACTGG CCGCAAGGGG
    AGGGTCGTAA GCAATGATGA CGGAAGCATA TCTTATGAGT CAAGATCTGA ACTTGATGTT
    CCTGTGGAAA TACTAAATAT TACAGAAAAA CAAAGATTTA TGGATGGAGA TAAGAATATT
    GCTATCATCT CAGAAGCTGC CAGCTCAGGT ATTTCATTAC AAGCGGATAG AAGGGCTAAA
    AATCAAAGGC GAAGAGTTCA TATGACTCTG GAGTTACCAT GGAGTGCTGA TAGAGCAATT
    CAGCAATTTG GACGAACTCA TAGATCAAAT CAAGTTACCG CTCCTGAATA TGTCTTTCTG
    ATTTCTGAAT TGGCAGGAGA ACAAAGATTT GCATCTATTG TTGCTAAAAG ACTTGAGAGT
    TTGGGCGCAC TTACACATGG AGACAGAAGA GCAACAGAAT CTAGAGATCT GAGCCGGTTC
    AACTTCGATA ATAAGTATGG AAGAAATGCT TTAGAAATTG TCATGAAATC CATTGTAAAC
    CTAGATTCTC CTATGGTATC ACCACCTCCA GACTATCCTG GAGAATTCTT TAAAGATGTT
    CGACAAGGAC TGATAGGAGT TGGTCTAATA AATGTAGAAG ATCGTTCAGG AATTCTTACT
    CTAGATAAAG ATTATAACAA CATAGGAAAA TTCTTAAACA GAATTTTGGG CATGGAAGTG
    CATCAACAGA ATGCACTATT TCAGTATTTC GCAGACACAC TTACTGCAGT CGTTCAAAAT
    GCCAAAAAAA ATGGAAGATA CGATATGGGA ATCTTAGATC TTGGTTCTGG GGATGAAAAA
    GTGAGGAAAA GTGATGTTAA GAAGTTTCTG ACTCCAGGAT ATTCAACATC TGGCCATGTA
    GAATTATACA CAATAAGTGT GGAGAGGGGA ATGTCCTGGG AGGAAGCTAC CAAGATTTGG
    GCTGAGCTGA CAGGACCAGA TGATGGCTTT TATTTGTCAT TGCAAATAAG AAACAACAAG
    AAAACTGCCA TCTTAGTTAA AGAAGTAAAC CCTAAAAAGA AGCTTTTCTT AGTTTATAGA
    CCAAATACCG GGAAACAACT CAAATTAGAA ATTTATGCTG ATCTAAAAAA GAAATATAAG
    AAGGTAGTCT CAGACGATGC CGTGGTCCAC TGGTTAGATC AGTATAATTC ATCTGCAGAT
    ACTTGTACTC ATGCTTATTG GCGTGGCAAC TGCAAAAAAG CAAGCTTGGG ATTAGTTTGT
    GAAATAGGTC TTCGTTGTCG CACATACTAT GTATTATGTG GCTCAGTACT GAGTGTCTGG
    ACAAAAGTTG AGGGTGTTCT TGCGTCTGTC AGTGGCACAA ATGTGAAAAT GCAGATAGTT
    CGCCTAAGAA CAGAGGATGG ACAGCGGATT GTAGGTTTGA TCATTCCTGC AAATTGTGTG
    TCTCCTCTTA TAAATCTCCT TTCAACTTCA GACCAGTCTC AACAGCTTGC AGTCCAGCAG
    AAACAGCTCT GGCAGCAGCG TCACCCCCAG AGCATTGCCA GCTTGAGCAG CACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812306.1
    CDS166..4242
    Translation

    Target ORF information:

    RefSeq Version XM_010814004.3
    Organism Bos taurus(cattle)
    Definition Bos taurus strawberry notch homolog 1 (SBNO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814004.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGCAGCAGCC TCCATCTACT ACAACATTTG TGCTGAATCA AATAAATCAG
    CTTCCAACCT TGGGATCTAC AATTGTAATG ACTAAGACAC CACCTGTAAC CACGAATAGG
    CAAACCATCA CTTTAACGAA GTTCATTCAG ACTACTGCAA ACACACGCCC TTCAGTCTCA
    GCACCAGCAG TACGAAATGC CATGACTTCC GCACCTTCAA AAGACCAAGT TCAACTGAAG
    GACCTGCTAA AAAATAATAG TCTGAATGAA CTCATGAAAC TGAAGCCACC TGCTAATATT
    GCTCAGCCAG TTGCAACAGC AGCTACTGAT GTAAGCAATG GCACAGTAAA GAAAGAGTCT
    TCTAATAAAG AAGTAGCAAG AATATGGATA AATGACATGA AAATGAGGAG TTTTTCCCCA
    ACCATGAAGG TCCCTGTTGT GAAAGAGGAA GATGAACCAG AAGAAGAAGA CGAAGAAGAA
    ATGGGTCATG CGGAAACCTA TGCAGAGTAT ATGCCAATAA AATTAAAAAT TGGCCTACGT
    CACCCAGATG CCGTAGTTGA GACTAGTTCT TTATCCAGTG TTACTCCTCC TGATGTTTGG
    TACAAGACAT CTATTTCTGA AGAAACCATT GATAATGGCT GGTTATCAGC ATTACAGCTT
    GAAGCAATTA CTTATGCAGC CCAGCAACAT GAAACGTTCC TACCTAATGG AGATCGTGCT
    GGCTTCTTAA TAGGCGATGG TGCTGGTGTA GGAAAAGGAA GGACAATAGC AGGAATCATC
    TATGAAAATT ATTTGTTGAG TAGAAAAAGG GCTTTGTGGT TCAGTGTTTC AAATGATTTA
    AAGTATGATG CTGAAAGAGA TTTGAGGGAT ATTGGAGCAA AAAACATTTT GGTCCATTCA
    TTGAATAAGT TTAAATATGG AAAAATTTCT TCCAAACATA ATGGGAGTGT GAAGAAGGGT
    GTTATTTTTG CTACCTATTC TTCCCTTATT GGTGAAAGTC AGTCTGGTGG TAAATATAAA
    ACGAGGTTAA AACAACTTCT ACATTGGTGC GGTGATGACT TCGATGGAGT GATAGTTTTT
    GATGAATGTC ATAAAGCAAA AAACTTATGT CCTGTTGGTT CTTCAAAGCC AACCAAGACA
    GGCTTAGCAG TTTTAGAACT TCAGAACAAA TTGCCAAAAG CTAGAGTTGT CTATGCTAGT
    GCCACTGGTG CTTCTGAACC ACGAAATATG GCCTATATGA ACCGTCTTGG AATATGGGGT
    GAGGGAACTC CATTTAGAGA ATTCAGTGAC TTTATTCAGG CAGTAGAACG GAGAGGTGTT
    GGTGCCATGG AAATAGTTGC TATGGATATG AAGCTTAGAG GAATGTACAT TGCTCGACAG
    CTGAGCTTTA CTGGAGTGAC CTTCAAAATT GAGGAAGTTC TTCTTTCTCA GAGCTATGTT
    AAAATGTATA ACAAAGCAGT CAAACTGTGG GTCATTGCTC GAGAGCGGTT TCAGCAAGCT
    GCAGATCTGA TTGATGCTGA GCAGAGAATG AAGAAATCCA TGTGGGGTCA GTTCTGGTCT
    GCTCACCAGA GATTTTTCAA ATACTTGTGC ATAGCGTCCA AAGTTAAAAG AGTTGTGCAA
    CTAGCTCGAG AAGAAATCAA GAATGGAAAA TGTGTTGTAA TTGGTCTGCA GTCTACGGGA
    GAAGCTAGAA CATTAGAAGC CTTGGAAGAG GGCGGGGGAG AATTGAATGA TTTTGTTTCA
    ACTGCCAAAG GAGTGTTACA GTCACTCATT GAAAAACACT TCCCTGCTCC GGATAGAAAA
    AAGCTTTATA GTTTGCTGGG AATTGATTTG ACAGCTCCAA GTAACAGCAG TTCACCAAGA
    GATAGTCCTT GTAAGGAAAA TAAAACAAAG AAGCGGAAAG GTGAAGAAAT AACTCGAGAA
    GCCAAAAAAG CACGAAAAAT AGGTGGCCTT ACTGGTAGCA GTTCTGATGA TAGTGGAAGT
    GAATCTGATG CCTCTGATAA TGAAGAAAGT GACTATGAGA GCTCTAAAAA CATGAGTTCT
    GGAGATGATG ATGATTTCAA CCCATTCAGA GATGAGTCTA GTGAGGATGA TGAAGATGAT
    CCCTGGTTAA TCAGAAAAGA TCATAAGAAA AACAAAGATA AAAAAAAAAG GAAAAGTATA
    GATCCAGATT CTATTCAAAG TGCCTTATTA GCGTCAGGTC TTGGATCAAA ACGACCTAGT
    TTTTCTTCTA CACCAGTTAT TTCACCTGCT CCTAACAGTG CACCAGCTAA CAGTAACACC
    AACAGCAACA GTAGCCTTAT AACAAGTCAA GATGCCGTGG AAAGGGCCCA ACAGATGAAG
    AAAGACCTAC TTGATAAACT AGAAAAATTA GCTGAAGACC TCCCTCCTAA TACCCTGGAT
    GAACTTATTG ATGAACTTGG TGGCCCTGAA AATGTTGCTG AGATGACTGG CCGCAAGGGG
    AGGGTCGTAA GCAATGATGA CGGAAGCATA TCTTATGAGT CAAGATCTGA ACTTGATGTT
    CCTGTGGAAA TACTAAATAT TACAGAAAAA CAAAGATTTA TGGATGGAGA TAAGAATATT
    GCTATCATCT CAGAAGCTGC CAGCTCAGGT ATTTCATTAC AAGCGGATAG AAGGGCTAAA
    AATCAAAGGC GAAGAGTTCA TATGACTCTG GAGTTACCAT GGAGTGCTGA TAGAGCAATT
    CAGCAATTTG GACGAACTCA TAGATCAAAT CAAGTTACCG CTCCTGAATA TGTCTTTCTG
    ATTTCTGAAT TGGCAGGAGA ACAAAGATTT GCATCTATTG TTGCTAAAAG ACTTGAGAGT
    TTGGGCGCAC TTACACATGG AGACAGAAGA GCAACAGAAT CTAGAGATCT GAGCCGGTTC
    AACTTCGATA ATAAGTATGG AAGAAATGCT TTAGAAATTG TCATGAAATC CATTGTAAAC
    CTAGATTCTC CTATGGTATC ACCACCTCCA GACTATCCTG GAGAATTCTT TAAAGATGTT
    CGACAAGGAC TGATAGGAGT TGGTCTAATA AATGTAGAAG ATCGTTCAGG AATTCTTACT
    CTAGATAAAG ATTATAACAA CATAGGAAAA TTCTTAAACA GAATTTTGGG CATGGAAGTG
    CATCAACAGA ATGCACTATT TCAGTATTTC GCAGACACAC TTACTGCAGT CGTTCAAAAT
    GCCAAAAAAA ATGGAAGATA CGATATGGGA ATCTTAGATC TTGGTTCTGG GGATGAAAAA
    GTGAGGAAAA GTGATGTTAA GAAGTTTCTG ACTCCAGGAT ATTCAACATC TGGCCATGTA
    GAATTATACA CAATAAGTGT GGAGAGGGGA ATGTCCTGGG AGGAAGCTAC CAAGATTTGG
    GCTGAGCTGA CAGGACCAGA TGATGGCTTT TATTTGTCAT TGCAAATAAG AAACAACAAG
    AAAACTGCCA TCTTAGTTAA AGAAGTAAAC CCTAAAAAGA AGCTTTTCTT AGTTTATAGA
    CCAAATACCG GGAAACAACT CAAATTAGAA ATTTATGCTG ATCTAAAAAA GAAATATAAG
    AAGGTAGTCT CAGACGATGC CGTGGTCCAC TGGTTAGATC AGTATAATTC ATCTGCAGAT
    ACTTGTACTC ATGCTTATTG GCGTGGCAAC TGCAAAAAAG CAAGCTTGGG ATTAGTTTGT
    GAAATAGGTC TTCGTTGTCG CACATACTAT GTATTATGTG GCTCAGTACT GAGTGTCTGG
    ACAAAAGTTG AGGGTGTTCT TGCGTCTGTC AGTGGCACAA ATGTGAAAAT GCAGATAGTT
    CGCCTAAGAA CAGAGGATGG ACAGCGGATT GTAGGTTTGA TCATTCCTGC AAATTGTGTG
    TCTCCTCTTA TAAATCTCCT TTCAACTTCA GACCAGTCTC AACAGCTTGC AGTCCAGCAG
    AAACAGCTCT GGCAGCAGCG TCACCCCCAG AGCATTGCCA GCTTGAGCAG CACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54722
    Clone ID Related Accession (Same CDS sequence) XM_010814005.3 , XM_010823359.2 , XM_010814005.2
    Accession Version XM_010814005.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein strawberry notch homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814005.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGCAGCCTCC ATCTACTACA ACATTTGTGC TGAATCAAAT AAATCAGCTT
    CCAACCTTGG GATCTACAAT TGTAATGACT AAGACACCAC CTGTAACCAC GAATAGGCAA
    ACCATCACTT TAACGAAGTT CATTCAGACT ACTGCAAACA CACGCCCTTC AGTCTCAGCA
    CCAGCAGTAC GAAATGCCAT GACTTCCGCA CCTTCAAAAG ACCAAGTTCA ACTGAAGGAC
    CTGCTAAAAA ATAATAGTCT GAATGAACTC ATGAAACTGA AGCCACCTGC TAATATTGCT
    CAGCCAGTTG CAACAGCAGC TACTGATGTA AGCAATGGCA CAGTAAAGAA AGAGTCTTCT
    AATAAAGAAG TAGCAAGAAT ATGGATAAAT GACATGAAAA TGAGGAGTTT TTCCCCAACC
    ATGAAGGTCC CTGTTGTGAA AGAGGAAGAT GAACCAGAAG AAGAAGACGA AGAAGAAATG
    GGTCATGCGG AAACCTATGC AGAGTATATG CCAATAAAAT TAAAAATTGG CCTACGTCAC
    CCAGATGCCG TAGTTGAGAC TAGTTCTTTA TCCAGTGTTA CTCCTCCTGA TGTTTGGTAC
    AAGACATCTA TTTCTGAAGA AACCATTGAT AATGGCTGGT TATCAGCATT ACAGCTTGAA
    GCAATTACTT ATGCAGCCCA GCAACATGAA ACGTTCCTAC CTAATGGAGA TCGTGCTGGC
    TTCTTAATAG GCGATGGTGC TGGTGTAGGA AAAGGAAGGA CAATAGCAGG AATCATCTAT
    GAAAATTATT TGTTGAGTAG AAAAAGGGCT TTGTGGTTCA GTGTTTCAAA TGATTTAAAG
    TATGATGCTG AAAGAGATTT GAGGGATATT GGAGCAAAAA ACATTTTGGT CCATTCATTG
    AATAAGTTTA AATATGGAAA AATTTCTTCC AAACATAATG GGAGTGTGAA GAAGGGTGTT
    ATTTTTGCTA CCTATTCTTC CCTTATTGGT GAAAGTCAGT CTGGTGGTAA ATATAAAACG
    AGGTTAAAAC AACTTCTACA TTGGTGCGGT GATGACTTCG ATGGAGTGAT AGTTTTTGAT
    GAATGTCATA AAGCAAAAAA CTTATGTCCT GTTGGTTCTT CAAAGCCAAC CAAGACAGGC
    TTAGCAGTTT TAGAACTTCA GAACAAATTG CCAAAAGCTA GAGTTGTCTA TGCTAGTGCC
    ACTGGTGCTT CTGAACCACG AAATATGGCC TATATGAACC GTCTTGGAAT ATGGGGTGAG
    GGAACTCCAT TTAGAGAATT CAGTGACTTT ATTCAGGCAG TAGAACGGAG AGGTGTTGGT
    GCCATGGAAA TAGTTGCTAT GGATATGAAG CTTAGAGGAA TGTACATTGC TCGACAGCTG
    AGCTTTACTG GAGTGACCTT CAAAATTGAG GAAGTTCTTC TTTCTCAGAG CTATGTTAAA
    ATGTATAACA AAGCAGTCAA ACTGTGGGTC ATTGCTCGAG AGCGGTTTCA GCAAGCTGCA
    GATCTGATTG ATGCTGAGCA GAGAATGAAG AAATCCATGT GGGGTCAGTT CTGGTCTGCT
    CACCAGAGAT TTTTCAAATA CTTGTGCATA GCGTCCAAAG TTAAAAGAGT TGTGCAACTA
    GCTCGAGAAG AAATCAAGAA TGGAAAATGT GTTGTAATTG GTCTGCAGTC TACGGGAGAA
    GCTAGAACAT TAGAAGCCTT GGAAGAGGGC GGGGGAGAAT TGAATGATTT TGTTTCAACT
    GCCAAAGGAG TGTTACAGTC ACTCATTGAA AAACACTTCC CTGCTCCGGA TAGAAAAAAG
    CTTTATAGTT TGCTGGGAAT TGATTTGACA GCTCCAAGTA ACAGCAGTTC ACCAAGAGAT
    AGTCCTTGTA AGGAAAATAA AACAAAGAAG CGGAAAGGTG AAGAAATAAC TCGAGAAGCC
    AAAAAAGCAC GAAAAATAGG TGGCCTTACT GGTAGCAGTT CTGATGATAG TGGAAGTGAA
    TCTGATGCCT CTGATAATGA AGAAAGTGAC TATGAGAGCT CTAAAAACAT GAGTTCTGGA
    GATGATGATG ATTTCAACCC ATTCAGAGAT GAGTCTAGTG AGGATGATGA AGATGATCCC
    TGGTTAATCA GAAAAGATCA TAAGAAAAAC AAAGATAAAA AAAAAAGGAA AAGTATAGAT
    CCAGATTCTA TTCAAAGTGC CTTATTAGCG TCAGGTCTTG GATCAAAACG ACCTAGTTTT
    TCTTCTACAC CAGTTATTTC ACCTGCTCCT AACAGTGCAC CAGCTAACAG TAACACCAAC
    AGCAACAGTA GCCTTATAAC AAGTCAAGAT GCCGTGGAAA GGGCCCAACA GATGAAGAAA
    GACCTACTTG ATAAACTAGA AAAATTAGCT GAAGACCTCC CTCCTAATAC CCTGGATGAA
    CTTATTGATG AACTTGGTGG CCCTGAAAAT GTTGCTGAGA TGACTGGCCG CAAGGGGAGG
    GTCGTAAGCA ATGATGACGG AAGCATATCT TATGAGTCAA GATCTGAACT TGATGTTCCT
    GTGGAAATAC TAAATATTAC AGAAAAACAA AGATTTATGG ATGGAGATAA GAATATTGCT
    ATCATCTCAG AAGCTGCCAG CTCAGGTATT TCATTACAAG CGGATAGAAG GGCTAAAAAT
    CAAAGGCGAA GAGTTCATAT GACTCTGGAG TTACCATGGA GTGCTGATAG AGCAATTCAG
    CAATTTGGAC GAACTCATAG ATCAAATCAA GTTACCGCTC CTGAATATGT CTTTCTGATT
    TCTGAATTGG CAGGAGAACA AAGATTTGCA TCTATTGTTG CTAAAAGACT TGAGAGTTTG
    GGCGCACTTA CACATGGAGA CAGAAGAGCA ACAGAATCTA GAGATCTGAG CCGGTTCAAC
    TTCGATAATA AGTATGGAAG AAATGCTTTA GAAATTGTCA TGAAATCCAT TGTAAACCTA
    GATTCTCCTA TGGTATCACC ACCTCCAGAC TATCCTGGAG AATTCTTTAA AGATGTTCGA
    CAAGGACTGA TAGGAGTTGG TCTAATAAAT GTAGAAGATC GTTCAGGAAT TCTTACTCTA
    GATAAAGATT ATAACAACAT AGGAAAATTC TTAAACAGAA TTTTGGGCAT GGAAGTGCAT
    CAACAGAATG CACTATTTCA GTATTTCGCA GACACACTTA CTGCAGTCGT TCAAAATGCC
    AAAAAAAATG GAAGATACGA TATGGGAATC TTAGATCTTG GTTCTGGGGA TGAAAAAGTG
    AGGAAAAGTG ATGTTAAGAA GTTTCTGACT CCAGGATATT CAACATCTGG CCATGTAGAA
    TTATACACAA TAAGTGTGGA GAGGGGAATG TCCTGGGAGG AAGCTACCAA GATTTGGGCT
    GAGCTGACAG GACCAGATGA TGGCTTTTAT TTGTCATTGC AAATAAGAAA CAACAAGAAA
    ACTGCCATCT TAGTTAAAGA AGTAAACCCT AAAAAGAAGC TTTTCTTAGT TTATAGACCA
    AATACCGGGA AACAACTCAA ATTAGAAATT TATGCTGATC TAAAAAAGAA ATATAAGAAG
    GTAGTCTCAG ACGATGCCGT GGTCCACTGG TTAGATCAGT ATAATTCATC TGCAGATACT
    TGTACTCATG CTTATTGGCG TGGCAACTGC AAAAAAGCAA GCTTGGGATT AGTTTGTGAA
    ATAGGTCTTC GTTGTCGCAC ATACTATGTA TTATGTGGCT CAGTACTGAG TGTCTGGACA
    AAAGTTGAGG GTGTTCTTGC GTCTGTCAGT GGCACAAATG TGAAAATGCA GATAGTTCGC
    CTAAGAACAG AGGATGGACA GCGGATTGTA GGTTTGATCA TTCCTGCAAA TTGTGTGTCT
    CCTCTTATAA ATCTCCTTTC AACTTCAGAC CAGTCTCAAC AGCTTGCAGT CCAGCAGAAA
    CAGCTCTGGC AGCAGCGTCA CCCCCAGAGC ATTGCCAGCT TGAGCAGCAC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812307.1
    CDS166..4239
    Translation

    Target ORF information:

    RefSeq Version XM_010814005.3
    Organism Bos taurus(cattle)
    Definition Bos taurus strawberry notch homolog 1 (SBNO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814005.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGCAGCCTCC ATCTACTACA ACATTTGTGC TGAATCAAAT AAATCAGCTT
    CCAACCTTGG GATCTACAAT TGTAATGACT AAGACACCAC CTGTAACCAC GAATAGGCAA
    ACCATCACTT TAACGAAGTT CATTCAGACT ACTGCAAACA CACGCCCTTC AGTCTCAGCA
    CCAGCAGTAC GAAATGCCAT GACTTCCGCA CCTTCAAAAG ACCAAGTTCA ACTGAAGGAC
    CTGCTAAAAA ATAATAGTCT GAATGAACTC ATGAAACTGA AGCCACCTGC TAATATTGCT
    CAGCCAGTTG CAACAGCAGC TACTGATGTA AGCAATGGCA CAGTAAAGAA AGAGTCTTCT
    AATAAAGAAG TAGCAAGAAT ATGGATAAAT GACATGAAAA TGAGGAGTTT TTCCCCAACC
    ATGAAGGTCC CTGTTGTGAA AGAGGAAGAT GAACCAGAAG AAGAAGACGA AGAAGAAATG
    GGTCATGCGG AAACCTATGC AGAGTATATG CCAATAAAAT TAAAAATTGG CCTACGTCAC
    CCAGATGCCG TAGTTGAGAC TAGTTCTTTA TCCAGTGTTA CTCCTCCTGA TGTTTGGTAC
    AAGACATCTA TTTCTGAAGA AACCATTGAT AATGGCTGGT TATCAGCATT ACAGCTTGAA
    GCAATTACTT ATGCAGCCCA GCAACATGAA ACGTTCCTAC CTAATGGAGA TCGTGCTGGC
    TTCTTAATAG GCGATGGTGC TGGTGTAGGA AAAGGAAGGA CAATAGCAGG AATCATCTAT
    GAAAATTATT TGTTGAGTAG AAAAAGGGCT TTGTGGTTCA GTGTTTCAAA TGATTTAAAG
    TATGATGCTG AAAGAGATTT GAGGGATATT GGAGCAAAAA ACATTTTGGT CCATTCATTG
    AATAAGTTTA AATATGGAAA AATTTCTTCC AAACATAATG GGAGTGTGAA GAAGGGTGTT
    ATTTTTGCTA CCTATTCTTC CCTTATTGGT GAAAGTCAGT CTGGTGGTAA ATATAAAACG
    AGGTTAAAAC AACTTCTACA TTGGTGCGGT GATGACTTCG ATGGAGTGAT AGTTTTTGAT
    GAATGTCATA AAGCAAAAAA CTTATGTCCT GTTGGTTCTT CAAAGCCAAC CAAGACAGGC
    TTAGCAGTTT TAGAACTTCA GAACAAATTG CCAAAAGCTA GAGTTGTCTA TGCTAGTGCC
    ACTGGTGCTT CTGAACCACG AAATATGGCC TATATGAACC GTCTTGGAAT ATGGGGTGAG
    GGAACTCCAT TTAGAGAATT CAGTGACTTT ATTCAGGCAG TAGAACGGAG AGGTGTTGGT
    GCCATGGAAA TAGTTGCTAT GGATATGAAG CTTAGAGGAA TGTACATTGC TCGACAGCTG
    AGCTTTACTG GAGTGACCTT CAAAATTGAG GAAGTTCTTC TTTCTCAGAG CTATGTTAAA
    ATGTATAACA AAGCAGTCAA ACTGTGGGTC ATTGCTCGAG AGCGGTTTCA GCAAGCTGCA
    GATCTGATTG ATGCTGAGCA GAGAATGAAG AAATCCATGT GGGGTCAGTT CTGGTCTGCT
    CACCAGAGAT TTTTCAAATA CTTGTGCATA GCGTCCAAAG TTAAAAGAGT TGTGCAACTA
    GCTCGAGAAG AAATCAAGAA TGGAAAATGT GTTGTAATTG GTCTGCAGTC TACGGGAGAA
    GCTAGAACAT TAGAAGCCTT GGAAGAGGGC GGGGGAGAAT TGAATGATTT TGTTTCAACT
    GCCAAAGGAG TGTTACAGTC ACTCATTGAA AAACACTTCC CTGCTCCGGA TAGAAAAAAG
    CTTTATAGTT TGCTGGGAAT TGATTTGACA GCTCCAAGTA ACAGCAGTTC ACCAAGAGAT
    AGTCCTTGTA AGGAAAATAA AACAAAGAAG CGGAAAGGTG AAGAAATAAC TCGAGAAGCC
    AAAAAAGCAC GAAAAATAGG TGGCCTTACT GGTAGCAGTT CTGATGATAG TGGAAGTGAA
    TCTGATGCCT CTGATAATGA AGAAAGTGAC TATGAGAGCT CTAAAAACAT GAGTTCTGGA
    GATGATGATG ATTTCAACCC ATTCAGAGAT GAGTCTAGTG AGGATGATGA AGATGATCCC
    TGGTTAATCA GAAAAGATCA TAAGAAAAAC AAAGATAAAA AAAAAAGGAA AAGTATAGAT
    CCAGATTCTA TTCAAAGTGC CTTATTAGCG TCAGGTCTTG GATCAAAACG ACCTAGTTTT
    TCTTCTACAC CAGTTATTTC ACCTGCTCCT AACAGTGCAC CAGCTAACAG TAACACCAAC
    AGCAACAGTA GCCTTATAAC AAGTCAAGAT GCCGTGGAAA GGGCCCAACA GATGAAGAAA
    GACCTACTTG ATAAACTAGA AAAATTAGCT GAAGACCTCC CTCCTAATAC CCTGGATGAA
    CTTATTGATG AACTTGGTGG CCCTGAAAAT GTTGCTGAGA TGACTGGCCG CAAGGGGAGG
    GTCGTAAGCA ATGATGACGG AAGCATATCT TATGAGTCAA GATCTGAACT TGATGTTCCT
    GTGGAAATAC TAAATATTAC AGAAAAACAA AGATTTATGG ATGGAGATAA GAATATTGCT
    ATCATCTCAG AAGCTGCCAG CTCAGGTATT TCATTACAAG CGGATAGAAG GGCTAAAAAT
    CAAAGGCGAA GAGTTCATAT GACTCTGGAG TTACCATGGA GTGCTGATAG AGCAATTCAG
    CAATTTGGAC GAACTCATAG ATCAAATCAA GTTACCGCTC CTGAATATGT CTTTCTGATT
    TCTGAATTGG CAGGAGAACA AAGATTTGCA TCTATTGTTG CTAAAAGACT TGAGAGTTTG
    GGCGCACTTA CACATGGAGA CAGAAGAGCA ACAGAATCTA GAGATCTGAG CCGGTTCAAC
    TTCGATAATA AGTATGGAAG AAATGCTTTA GAAATTGTCA TGAAATCCAT TGTAAACCTA
    GATTCTCCTA TGGTATCACC ACCTCCAGAC TATCCTGGAG AATTCTTTAA AGATGTTCGA
    CAAGGACTGA TAGGAGTTGG TCTAATAAAT GTAGAAGATC GTTCAGGAAT TCTTACTCTA
    GATAAAGATT ATAACAACAT AGGAAAATTC TTAAACAGAA TTTTGGGCAT GGAAGTGCAT
    CAACAGAATG CACTATTTCA GTATTTCGCA GACACACTTA CTGCAGTCGT TCAAAATGCC
    AAAAAAAATG GAAGATACGA TATGGGAATC TTAGATCTTG GTTCTGGGGA TGAAAAAGTG
    AGGAAAAGTG ATGTTAAGAA GTTTCTGACT CCAGGATATT CAACATCTGG CCATGTAGAA
    TTATACACAA TAAGTGTGGA GAGGGGAATG TCCTGGGAGG AAGCTACCAA GATTTGGGCT
    GAGCTGACAG GACCAGATGA TGGCTTTTAT TTGTCATTGC AAATAAGAAA CAACAAGAAA
    ACTGCCATCT TAGTTAAAGA AGTAAACCCT AAAAAGAAGC TTTTCTTAGT TTATAGACCA
    AATACCGGGA AACAACTCAA ATTAGAAATT TATGCTGATC TAAAAAAGAA ATATAAGAAG
    GTAGTCTCAG ACGATGCCGT GGTCCACTGG TTAGATCAGT ATAATTCATC TGCAGATACT
    TGTACTCATG CTTATTGGCG TGGCAACTGC AAAAAAGCAA GCTTGGGATT AGTTTGTGAA
    ATAGGTCTTC GTTGTCGCAC ATACTATGTA TTATGTGGCT CAGTACTGAG TGTCTGGACA
    AAAGTTGAGG GTGTTCTTGC GTCTGTCAGT GGCACAAATG TGAAAATGCA GATAGTTCGC
    CTAAGAACAG AGGATGGACA GCGGATTGTA GGTTTGATCA TTCCTGCAAA TTGTGTGTCT
    CCTCTTATAA ATCTCCTTTC AACTTCAGAC CAGTCTCAAC AGCTTGCAGT CCAGCAGAAA
    CAGCTCTGGC AGCAGCGTCA CCCCCAGAGC ATTGCCAGCT TGAGCAGCAC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54722
    Clone ID Related Accession (Same CDS sequence) XM_010814005.3 , XM_010823359.2 , XM_010814005.2
    Accession Version XM_010823359.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein strawberry notch homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGCAGCCTCC ATCTACTACA ACATTTGTGC TGAATCAAAT AAATCAGCTT
    CCAACCTTGG GATCTACAAT TGTAATGACT AAGACACCAC CTGTAACCAC GAATAGGCAA
    ACCATCACTT TAACGAAGTT CATTCAGACT ACTGCAAACA CACGCCCTTC AGTCTCAGCA
    CCAGCAGTAC GAAATGCCAT GACTTCCGCA CCTTCAAAAG ACCAAGTTCA ACTGAAGGAC
    CTGCTAAAAA ATAATAGTCT GAATGAACTC ATGAAACTGA AGCCACCTGC TAATATTGCT
    CAGCCAGTTG CAACAGCAGC TACTGATGTA AGCAATGGCA CAGTAAAGAA AGAGTCTTCT
    AATAAAGAAG TAGCAAGAAT ATGGATAAAT GACATGAAAA TGAGGAGTTT TTCCCCAACC
    ATGAAGGTCC CTGTTGTGAA AGAGGAAGAT GAACCAGAAG AAGAAGACGA AGAAGAAATG
    GGTCATGCGG AAACCTATGC AGAGTATATG CCAATAAAAT TAAAAATTGG CCTACGTCAC
    CCAGATGCCG TAGTTGAGAC TAGTTCTTTA TCCAGTGTTA CTCCTCCTGA TGTTTGGTAC
    AAGACATCTA TTTCTGAAGA AACCATTGAT AATGGCTGGT TATCAGCATT ACAGCTTGAA
    GCAATTACTT ATGCAGCCCA GCAACATGAA ACGTTCCTAC CTAATGGAGA TCGTGCTGGC
    TTCTTAATAG GCGATGGTGC TGGTGTAGGA AAAGGAAGGA CAATAGCAGG AATCATCTAT
    GAAAATTATT TGTTGAGTAG AAAAAGGGCT TTGTGGTTCA GTGTTTCAAA TGATTTAAAG
    TATGATGCTG AAAGAGATTT GAGGGATATT GGAGCAAAAA ACATTTTGGT CCATTCATTG
    AATAAGTTTA AATATGGAAA AATTTCTTCC AAACATAATG GGAGTGTGAA GAAGGGTGTT
    ATTTTTGCTA CCTATTCTTC CCTTATTGGT GAAAGTCAGT CTGGTGGTAA ATATAAAACG
    AGGTTAAAAC AACTTCTACA TTGGTGCGGT GATGACTTCG ATGGAGTGAT AGTTTTTGAT
    GAATGTCATA AAGCAAAAAA CTTATGTCCT GTTGGTTCTT CAAAGCCAAC CAAGACAGGC
    TTAGCAGTTT TAGAACTTCA GAACAAATTG CCAAAAGCTA GAGTTGTCTA TGCTAGTGCC
    ACTGGTGCTT CTGAACCACG AAATATGGCC TATATGAACC GTCTTGGAAT ATGGGGTGAG
    GGAACTCCAT TTAGAGAATT CAGTGACTTT ATTCAGGCAG TAGAACGGAG AGGTGTTGGT
    GCCATGGAAA TAGTTGCTAT GGATATGAAG CTTAGAGGAA TGTACATTGC TCGACAGCTG
    AGCTTTACTG GAGTGACCTT CAAAATTGAG GAAGTTCTTC TTTCTCAGAG CTATGTTAAA
    ATGTATAACA AAGCAGTCAA ACTGTGGGTC ATTGCTCGAG AGCGGTTTCA GCAAGCTGCA
    GATCTGATTG ATGCTGAGCA GAGAATGAAG AAATCCATGT GGGGTCAGTT CTGGTCTGCT
    CACCAGAGAT TTTTCAAATA CTTGTGCATA GCGTCCAAAG TTAAAAGAGT TGTGCAACTA
    GCTCGAGAAG AAATCAAGAA TGGAAAATGT GTTGTAATTG GTCTGCAGTC TACGGGAGAA
    GCTAGAACAT TAGAAGCCTT GGAAGAGGGC GGGGGAGAAT TGAATGATTT TGTTTCAACT
    GCCAAAGGAG TGTTACAGTC ACTCATTGAA AAACACTTCC CTGCTCCGGA TAGAAAAAAG
    CTTTATAGTT TGCTGGGAAT TGATTTGACA GCTCCAAGTA ACAGCAGTTC ACCAAGAGAT
    AGTCCTTGTA AGGAAAATAA AACAAAGAAG CGGAAAGGTG AAGAAATAAC TCGAGAAGCC
    AAAAAAGCAC GAAAAATAGG TGGCCTTACT GGTAGCAGTT CTGATGATAG TGGAAGTGAA
    TCTGATGCCT CTGATAATGA AGAAAGTGAC TATGAGAGCT CTAAAAACAT GAGTTCTGGA
    GATGATGATG ATTTCAACCC ATTCAGAGAT GAGTCTAGTG AGGATGATGA AGATGATCCC
    TGGTTAATCA GAAAAGATCA TAAGAAAAAC AAAGATAAAA AAAAAAGGAA AAGTATAGAT
    CCAGATTCTA TTCAAAGTGC CTTATTAGCG TCAGGTCTTG GATCAAAACG ACCTAGTTTT
    TCTTCTACAC CAGTTATTTC ACCTGCTCCT AACAGTGCAC CAGCTAACAG TAACACCAAC
    AGCAACAGTA GCCTTATAAC AAGTCAAGAT GCCGTGGAAA GGGCCCAACA GATGAAGAAA
    GACCTACTTG ATAAACTAGA AAAATTAGCT GAAGACCTCC CTCCTAATAC CCTGGATGAA
    CTTATTGATG AACTTGGTGG CCCTGAAAAT GTTGCTGAGA TGACTGGCCG CAAGGGGAGG
    GTCGTAAGCA ATGATGACGG AAGCATATCT TATGAGTCAA GATCTGAACT TGATGTTCCT
    GTGGAAATAC TAAATATTAC AGAAAAACAA AGATTTATGG ATGGAGATAA GAATATTGCT
    ATCATCTCAG AAGCTGCCAG CTCAGGTATT TCATTACAAG CGGATAGAAG GGCTAAAAAT
    CAAAGGCGAA GAGTTCATAT GACTCTGGAG TTACCATGGA GTGCTGATAG AGCAATTCAG
    CAATTTGGAC GAACTCATAG ATCAAATCAA GTTACCGCTC CTGAATATGT CTTTCTGATT
    TCTGAATTGG CAGGAGAACA AAGATTTGCA TCTATTGTTG CTAAAAGACT TGAGAGTTTG
    GGCGCACTTA CACATGGAGA CAGAAGAGCA ACAGAATCTA GAGATCTGAG CCGGTTCAAC
    TTCGATAATA AGTATGGAAG AAATGCTTTA GAAATTGTCA TGAAATCCAT TGTAAACCTA
    GATTCTCCTA TGGTATCACC ACCTCCAGAC TATCCTGGAG AATTCTTTAA AGATGTTCGA
    CAAGGACTGA TAGGAGTTGG TCTAATAAAT GTAGAAGATC GTTCAGGAAT TCTTACTCTA
    GATAAAGATT ATAACAACAT AGGAAAATTC TTAAACAGAA TTTTGGGCAT GGAAGTGCAT
    CAACAGAATG CACTATTTCA GTATTTCGCA GACACACTTA CTGCAGTCGT TCAAAATGCC
    AAAAAAAATG GAAGATACGA TATGGGAATC TTAGATCTTG GTTCTGGGGA TGAAAAAGTG
    AGGAAAAGTG ATGTTAAGAA GTTTCTGACT CCAGGATATT CAACATCTGG CCATGTAGAA
    TTATACACAA TAAGTGTGGA GAGGGGAATG TCCTGGGAGG AAGCTACCAA GATTTGGGCT
    GAGCTGACAG GACCAGATGA TGGCTTTTAT TTGTCATTGC AAATAAGAAA CAACAAGAAA
    ACTGCCATCT TAGTTAAAGA AGTAAACCCT AAAAAGAAGC TTTTCTTAGT TTATAGACCA
    AATACCGGGA AACAACTCAA ATTAGAAATT TATGCTGATC TAAAAAAGAA ATATAAGAAG
    GTAGTCTCAG ACGATGCCGT GGTCCACTGG TTAGATCAGT ATAATTCATC TGCAGATACT
    TGTACTCATG CTTATTGGCG TGGCAACTGC AAAAAAGCAA GCTTGGGATT AGTTTGTGAA
    ATAGGTCTTC GTTGTCGCAC ATACTATGTA TTATGTGGCT CAGTACTGAG TGTCTGGACA
    AAAGTTGAGG GTGTTCTTGC GTCTGTCAGT GGCACAAATG TGAAAATGCA GATAGTTCGC
    CTAAGAACAG AGGATGGACA GCGGATTGTA GGTTTGATCA TTCCTGCAAA TTGTGTGTCT
    CCTCTTATAA ATCTCCTTTC AACTTCAGAC CAGTCTCAAC AGCTTGCAGT CCAGCAGAAA
    CAGCTCTGGC AGCAGCGTCA CCCCCAGAGC ATTGCCAGCT TGAGCAGCAC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821661.1
    CDS136..4209
    Translation

    Target ORF information:

    RefSeq Version XM_010823359.2
    Organism Bos taurus(cattle)
    Definition Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823359.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGCAGCCTCC ATCTACTACA ACATTTGTGC TGAATCAAAT AAATCAGCTT
    CCAACCTTGG GATCTACAAT TGTAATGACT AAGACACCAC CTGTAACCAC GAATAGGCAA
    ACCATCACTT TAACGAAGTT CATTCAGACT ACTGCAAACA CACGCCCTTC AGTCTCAGCA
    CCAGCAGTAC GAAATGCCAT GACTTCCGCA CCTTCAAAAG ACCAAGTTCA ACTGAAGGAC
    CTGCTAAAAA ATAATAGTCT GAATGAACTC ATGAAACTGA AGCCACCTGC TAATATTGCT
    CAGCCAGTTG CAACAGCAGC TACTGATGTA AGCAATGGCA CAGTAAAGAA AGAGTCTTCT
    AATAAAGAAG TAGCAAGAAT ATGGATAAAT GACATGAAAA TGAGGAGTTT TTCCCCAACC
    ATGAAGGTCC CTGTTGTGAA AGAGGAAGAT GAACCAGAAG AAGAAGACGA AGAAGAAATG
    GGTCATGCGG AAACCTATGC AGAGTATATG CCAATAAAAT TAAAAATTGG CCTACGTCAC
    CCAGATGCCG TAGTTGAGAC TAGTTCTTTA TCCAGTGTTA CTCCTCCTGA TGTTTGGTAC
    AAGACATCTA TTTCTGAAGA AACCATTGAT AATGGCTGGT TATCAGCATT ACAGCTTGAA
    GCAATTACTT ATGCAGCCCA GCAACATGAA ACGTTCCTAC CTAATGGAGA TCGTGCTGGC
    TTCTTAATAG GCGATGGTGC TGGTGTAGGA AAAGGAAGGA CAATAGCAGG AATCATCTAT
    GAAAATTATT TGTTGAGTAG AAAAAGGGCT TTGTGGTTCA GTGTTTCAAA TGATTTAAAG
    TATGATGCTG AAAGAGATTT GAGGGATATT GGAGCAAAAA ACATTTTGGT CCATTCATTG
    AATAAGTTTA AATATGGAAA AATTTCTTCC AAACATAATG GGAGTGTGAA GAAGGGTGTT
    ATTTTTGCTA CCTATTCTTC CCTTATTGGT GAAAGTCAGT CTGGTGGTAA ATATAAAACG
    AGGTTAAAAC AACTTCTACA TTGGTGCGGT GATGACTTCG ATGGAGTGAT AGTTTTTGAT
    GAATGTCATA AAGCAAAAAA CTTATGTCCT GTTGGTTCTT CAAAGCCAAC CAAGACAGGC
    TTAGCAGTTT TAGAACTTCA GAACAAATTG CCAAAAGCTA GAGTTGTCTA TGCTAGTGCC
    ACTGGTGCTT CTGAACCACG AAATATGGCC TATATGAACC GTCTTGGAAT ATGGGGTGAG
    GGAACTCCAT TTAGAGAATT CAGTGACTTT ATTCAGGCAG TAGAACGGAG AGGTGTTGGT
    GCCATGGAAA TAGTTGCTAT GGATATGAAG CTTAGAGGAA TGTACATTGC TCGACAGCTG
    AGCTTTACTG GAGTGACCTT CAAAATTGAG GAAGTTCTTC TTTCTCAGAG CTATGTTAAA
    ATGTATAACA AAGCAGTCAA ACTGTGGGTC ATTGCTCGAG AGCGGTTTCA GCAAGCTGCA
    GATCTGATTG ATGCTGAGCA GAGAATGAAG AAATCCATGT GGGGTCAGTT CTGGTCTGCT
    CACCAGAGAT TTTTCAAATA CTTGTGCATA GCGTCCAAAG TTAAAAGAGT TGTGCAACTA
    GCTCGAGAAG AAATCAAGAA TGGAAAATGT GTTGTAATTG GTCTGCAGTC TACGGGAGAA
    GCTAGAACAT TAGAAGCCTT GGAAGAGGGC GGGGGAGAAT TGAATGATTT TGTTTCAACT
    GCCAAAGGAG TGTTACAGTC ACTCATTGAA AAACACTTCC CTGCTCCGGA TAGAAAAAAG
    CTTTATAGTT TGCTGGGAAT TGATTTGACA GCTCCAAGTA ACAGCAGTTC ACCAAGAGAT
    AGTCCTTGTA AGGAAAATAA AACAAAGAAG CGGAAAGGTG AAGAAATAAC TCGAGAAGCC
    AAAAAAGCAC GAAAAATAGG TGGCCTTACT GGTAGCAGTT CTGATGATAG TGGAAGTGAA
    TCTGATGCCT CTGATAATGA AGAAAGTGAC TATGAGAGCT CTAAAAACAT GAGTTCTGGA
    GATGATGATG ATTTCAACCC ATTCAGAGAT GAGTCTAGTG AGGATGATGA AGATGATCCC
    TGGTTAATCA GAAAAGATCA TAAGAAAAAC AAAGATAAAA AAAAAAGGAA AAGTATAGAT
    CCAGATTCTA TTCAAAGTGC CTTATTAGCG TCAGGTCTTG GATCAAAACG ACCTAGTTTT
    TCTTCTACAC CAGTTATTTC ACCTGCTCCT AACAGTGCAC CAGCTAACAG TAACACCAAC
    AGCAACAGTA GCCTTATAAC AAGTCAAGAT GCCGTGGAAA GGGCCCAACA GATGAAGAAA
    GACCTACTTG ATAAACTAGA AAAATTAGCT GAAGACCTCC CTCCTAATAC CCTGGATGAA
    CTTATTGATG AACTTGGTGG CCCTGAAAAT GTTGCTGAGA TGACTGGCCG CAAGGGGAGG
    GTCGTAAGCA ATGATGACGG AAGCATATCT TATGAGTCAA GATCTGAACT TGATGTTCCT
    GTGGAAATAC TAAATATTAC AGAAAAACAA AGATTTATGG ATGGAGATAA GAATATTGCT
    ATCATCTCAG AAGCTGCCAG CTCAGGTATT TCATTACAAG CGGATAGAAG GGCTAAAAAT
    CAAAGGCGAA GAGTTCATAT GACTCTGGAG TTACCATGGA GTGCTGATAG AGCAATTCAG
    CAATTTGGAC GAACTCATAG ATCAAATCAA GTTACCGCTC CTGAATATGT CTTTCTGATT
    TCTGAATTGG CAGGAGAACA AAGATTTGCA TCTATTGTTG CTAAAAGACT TGAGAGTTTG
    GGCGCACTTA CACATGGAGA CAGAAGAGCA ACAGAATCTA GAGATCTGAG CCGGTTCAAC
    TTCGATAATA AGTATGGAAG AAATGCTTTA GAAATTGTCA TGAAATCCAT TGTAAACCTA
    GATTCTCCTA TGGTATCACC ACCTCCAGAC TATCCTGGAG AATTCTTTAA AGATGTTCGA
    CAAGGACTGA TAGGAGTTGG TCTAATAAAT GTAGAAGATC GTTCAGGAAT TCTTACTCTA
    GATAAAGATT ATAACAACAT AGGAAAATTC TTAAACAGAA TTTTGGGCAT GGAAGTGCAT
    CAACAGAATG CACTATTTCA GTATTTCGCA GACACACTTA CTGCAGTCGT TCAAAATGCC
    AAAAAAAATG GAAGATACGA TATGGGAATC TTAGATCTTG GTTCTGGGGA TGAAAAAGTG
    AGGAAAAGTG ATGTTAAGAA GTTTCTGACT CCAGGATATT CAACATCTGG CCATGTAGAA
    TTATACACAA TAAGTGTGGA GAGGGGAATG TCCTGGGAGG AAGCTACCAA GATTTGGGCT
    GAGCTGACAG GACCAGATGA TGGCTTTTAT TTGTCATTGC AAATAAGAAA CAACAAGAAA
    ACTGCCATCT TAGTTAAAGA AGTAAACCCT AAAAAGAAGC TTTTCTTAGT TTATAGACCA
    AATACCGGGA AACAACTCAA ATTAGAAATT TATGCTGATC TAAAAAAGAA ATATAAGAAG
    GTAGTCTCAG ACGATGCCGT GGTCCACTGG TTAGATCAGT ATAATTCATC TGCAGATACT
    TGTACTCATG CTTATTGGCG TGGCAACTGC AAAAAAGCAA GCTTGGGATT AGTTTGTGAA
    ATAGGTCTTC GTTGTCGCAC ATACTATGTA TTATGTGGCT CAGTACTGAG TGTCTGGACA
    AAAGTTGAGG GTGTTCTTGC GTCTGTCAGT GGCACAAATG TGAAAATGCA GATAGTTCGC
    CTAAGAACAG AGGATGGACA GCGGATTGTA GGTTTGATCA TTCCTGCAAA TTGTGTGTCT
    CCTCTTATAA ATCTCCTTTC AACTTCAGAC CAGTCTCAAC AGCTTGCAGT CCAGCAGAAA
    CAGCTCTGGC AGCAGCGTCA CCCCCAGAGC ATTGCCAGCT TGAGCAGCAC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54722
    Clone ID Related Accession (Same CDS sequence) XM_010814005.3 , XM_010823359.2 , XM_010814005.2
    Accession Version XM_010814005.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein strawberry notch homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGCAGCCTCC ATCTACTACA ACATTTGTGC TGAATCAAAT AAATCAGCTT
    CCAACCTTGG GATCTACAAT TGTAATGACT AAGACACCAC CTGTAACCAC GAATAGGCAA
    ACCATCACTT TAACGAAGTT CATTCAGACT ACTGCAAACA CACGCCCTTC AGTCTCAGCA
    CCAGCAGTAC GAAATGCCAT GACTTCCGCA CCTTCAAAAG ACCAAGTTCA ACTGAAGGAC
    CTGCTAAAAA ATAATAGTCT GAATGAACTC ATGAAACTGA AGCCACCTGC TAATATTGCT
    CAGCCAGTTG CAACAGCAGC TACTGATGTA AGCAATGGCA CAGTAAAGAA AGAGTCTTCT
    AATAAAGAAG TAGCAAGAAT ATGGATAAAT GACATGAAAA TGAGGAGTTT TTCCCCAACC
    ATGAAGGTCC CTGTTGTGAA AGAGGAAGAT GAACCAGAAG AAGAAGACGA AGAAGAAATG
    GGTCATGCGG AAACCTATGC AGAGTATATG CCAATAAAAT TAAAAATTGG CCTACGTCAC
    CCAGATGCCG TAGTTGAGAC TAGTTCTTTA TCCAGTGTTA CTCCTCCTGA TGTTTGGTAC
    AAGACATCTA TTTCTGAAGA AACCATTGAT AATGGCTGGT TATCAGCATT ACAGCTTGAA
    GCAATTACTT ATGCAGCCCA GCAACATGAA ACGTTCCTAC CTAATGGAGA TCGTGCTGGC
    TTCTTAATAG GCGATGGTGC TGGTGTAGGA AAAGGAAGGA CAATAGCAGG AATCATCTAT
    GAAAATTATT TGTTGAGTAG AAAAAGGGCT TTGTGGTTCA GTGTTTCAAA TGATTTAAAG
    TATGATGCTG AAAGAGATTT GAGGGATATT GGAGCAAAAA ACATTTTGGT CCATTCATTG
    AATAAGTTTA AATATGGAAA AATTTCTTCC AAACATAATG GGAGTGTGAA GAAGGGTGTT
    ATTTTTGCTA CCTATTCTTC CCTTATTGGT GAAAGTCAGT CTGGTGGTAA ATATAAAACG
    AGGTTAAAAC AACTTCTACA TTGGTGCGGT GATGACTTCG ATGGAGTGAT AGTTTTTGAT
    GAATGTCATA AAGCAAAAAA CTTATGTCCT GTTGGTTCTT CAAAGCCAAC CAAGACAGGC
    TTAGCAGTTT TAGAACTTCA GAACAAATTG CCAAAAGCTA GAGTTGTCTA TGCTAGTGCC
    ACTGGTGCTT CTGAACCACG AAATATGGCC TATATGAACC GTCTTGGAAT ATGGGGTGAG
    GGAACTCCAT TTAGAGAATT CAGTGACTTT ATTCAGGCAG TAGAACGGAG AGGTGTTGGT
    GCCATGGAAA TAGTTGCTAT GGATATGAAG CTTAGAGGAA TGTACATTGC TCGACAGCTG
    AGCTTTACTG GAGTGACCTT CAAAATTGAG GAAGTTCTTC TTTCTCAGAG CTATGTTAAA
    ATGTATAACA AAGCAGTCAA ACTGTGGGTC ATTGCTCGAG AGCGGTTTCA GCAAGCTGCA
    GATCTGATTG ATGCTGAGCA GAGAATGAAG AAATCCATGT GGGGTCAGTT CTGGTCTGCT
    CACCAGAGAT TTTTCAAATA CTTGTGCATA GCGTCCAAAG TTAAAAGAGT TGTGCAACTA
    GCTCGAGAAG AAATCAAGAA TGGAAAATGT GTTGTAATTG GTCTGCAGTC TACGGGAGAA
    GCTAGAACAT TAGAAGCCTT GGAAGAGGGC GGGGGAGAAT TGAATGATTT TGTTTCAACT
    GCCAAAGGAG TGTTACAGTC ACTCATTGAA AAACACTTCC CTGCTCCGGA TAGAAAAAAG
    CTTTATAGTT TGCTGGGAAT TGATTTGACA GCTCCAAGTA ACAGCAGTTC ACCAAGAGAT
    AGTCCTTGTA AGGAAAATAA AACAAAGAAG CGGAAAGGTG AAGAAATAAC TCGAGAAGCC
    AAAAAAGCAC GAAAAATAGG TGGCCTTACT GGTAGCAGTT CTGATGATAG TGGAAGTGAA
    TCTGATGCCT CTGATAATGA AGAAAGTGAC TATGAGAGCT CTAAAAACAT GAGTTCTGGA
    GATGATGATG ATTTCAACCC ATTCAGAGAT GAGTCTAGTG AGGATGATGA AGATGATCCC
    TGGTTAATCA GAAAAGATCA TAAGAAAAAC AAAGATAAAA AAAAAAGGAA AAGTATAGAT
    CCAGATTCTA TTCAAAGTGC CTTATTAGCG TCAGGTCTTG GATCAAAACG ACCTAGTTTT
    TCTTCTACAC CAGTTATTTC ACCTGCTCCT AACAGTGCAC CAGCTAACAG TAACACCAAC
    AGCAACAGTA GCCTTATAAC AAGTCAAGAT GCCGTGGAAA GGGCCCAACA GATGAAGAAA
    GACCTACTTG ATAAACTAGA AAAATTAGCT GAAGACCTCC CTCCTAATAC CCTGGATGAA
    CTTATTGATG AACTTGGTGG CCCTGAAAAT GTTGCTGAGA TGACTGGCCG CAAGGGGAGG
    GTCGTAAGCA ATGATGACGG AAGCATATCT TATGAGTCAA GATCTGAACT TGATGTTCCT
    GTGGAAATAC TAAATATTAC AGAAAAACAA AGATTTATGG ATGGAGATAA GAATATTGCT
    ATCATCTCAG AAGCTGCCAG CTCAGGTATT TCATTACAAG CGGATAGAAG GGCTAAAAAT
    CAAAGGCGAA GAGTTCATAT GACTCTGGAG TTACCATGGA GTGCTGATAG AGCAATTCAG
    CAATTTGGAC GAACTCATAG ATCAAATCAA GTTACCGCTC CTGAATATGT CTTTCTGATT
    TCTGAATTGG CAGGAGAACA AAGATTTGCA TCTATTGTTG CTAAAAGACT TGAGAGTTTG
    GGCGCACTTA CACATGGAGA CAGAAGAGCA ACAGAATCTA GAGATCTGAG CCGGTTCAAC
    TTCGATAATA AGTATGGAAG AAATGCTTTA GAAATTGTCA TGAAATCCAT TGTAAACCTA
    GATTCTCCTA TGGTATCACC ACCTCCAGAC TATCCTGGAG AATTCTTTAA AGATGTTCGA
    CAAGGACTGA TAGGAGTTGG TCTAATAAAT GTAGAAGATC GTTCAGGAAT TCTTACTCTA
    GATAAAGATT ATAACAACAT AGGAAAATTC TTAAACAGAA TTTTGGGCAT GGAAGTGCAT
    CAACAGAATG CACTATTTCA GTATTTCGCA GACACACTTA CTGCAGTCGT TCAAAATGCC
    AAAAAAAATG GAAGATACGA TATGGGAATC TTAGATCTTG GTTCTGGGGA TGAAAAAGTG
    AGGAAAAGTG ATGTTAAGAA GTTTCTGACT CCAGGATATT CAACATCTGG CCATGTAGAA
    TTATACACAA TAAGTGTGGA GAGGGGAATG TCCTGGGAGG AAGCTACCAA GATTTGGGCT
    GAGCTGACAG GACCAGATGA TGGCTTTTAT TTGTCATTGC AAATAAGAAA CAACAAGAAA
    ACTGCCATCT TAGTTAAAGA AGTAAACCCT AAAAAGAAGC TTTTCTTAGT TTATAGACCA
    AATACCGGGA AACAACTCAA ATTAGAAATT TATGCTGATC TAAAAAAGAA ATATAAGAAG
    GTAGTCTCAG ACGATGCCGT GGTCCACTGG TTAGATCAGT ATAATTCATC TGCAGATACT
    TGTACTCATG CTTATTGGCG TGGCAACTGC AAAAAAGCAA GCTTGGGATT AGTTTGTGAA
    ATAGGTCTTC GTTGTCGCAC ATACTATGTA TTATGTGGCT CAGTACTGAG TGTCTGGACA
    AAAGTTGAGG GTGTTCTTGC GTCTGTCAGT GGCACAAATG TGAAAATGCA GATAGTTCGC
    CTAAGAACAG AGGATGGACA GCGGATTGTA GGTTTGATCA TTCCTGCAAA TTGTGTGTCT
    CCTCTTATAA ATCTCCTTTC AACTTCAGAC CAGTCTCAAC AGCTTGCAGT CCAGCAGAAA
    CAGCTCTGGC AGCAGCGTCA CCCCCAGAGC ATTGCCAGCT TGAGCAGCAC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812307.1
    CDS136..4209
    Translation

    Target ORF information:

    RefSeq Version XM_010814005.2
    Organism Bos taurus(cattle)
    Definition Bos taurus strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814005.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGTGGAAC CAGGGCAAGA TTTACTGCTT GCTGCTTTGA GCGAGAGTGG AATTAGTCCA 
    AATGACCTCT TTGATATTGA TGGTGGAGAT GCGGGGCTTG CAACTCCAAC ACCTACCCCT
    CCCATACAGC AGCAGCCTCC ATCTACTACA ACATTTGTGC TGAATCAAAT AAATCAGCTT
    CCAACCTTGG GATCTACAAT TGTAATGACT AAGACACCAC CTGTAACCAC GAATAGGCAA
    ACCATCACTT TAACGAAGTT CATTCAGACT ACTGCAAACA CACGCCCTTC AGTCTCAGCA
    CCAGCAGTAC GAAATGCCAT GACTTCCGCA CCTTCAAAAG ACCAAGTTCA ACTGAAGGAC
    CTGCTAAAAA ATAATAGTCT GAATGAACTC ATGAAACTGA AGCCACCTGC TAATATTGCT
    CAGCCAGTTG CAACAGCAGC TACTGATGTA AGCAATGGCA CAGTAAAGAA AGAGTCTTCT
    AATAAAGAAG TAGCAAGAAT ATGGATAAAT GACATGAAAA TGAGGAGTTT TTCCCCAACC
    ATGAAGGTCC CTGTTGTGAA AGAGGAAGAT GAACCAGAAG AAGAAGACGA AGAAGAAATG
    GGTCATGCGG AAACCTATGC AGAGTATATG CCAATAAAAT TAAAAATTGG CCTACGTCAC
    CCAGATGCCG TAGTTGAGAC TAGTTCTTTA TCCAGTGTTA CTCCTCCTGA TGTTTGGTAC
    AAGACATCTA TTTCTGAAGA AACCATTGAT AATGGCTGGT TATCAGCATT ACAGCTTGAA
    GCAATTACTT ATGCAGCCCA GCAACATGAA ACGTTCCTAC CTAATGGAGA TCGTGCTGGC
    TTCTTAATAG GCGATGGTGC TGGTGTAGGA AAAGGAAGGA CAATAGCAGG AATCATCTAT
    GAAAATTATT TGTTGAGTAG AAAAAGGGCT TTGTGGTTCA GTGTTTCAAA TGATTTAAAG
    TATGATGCTG AAAGAGATTT GAGGGATATT GGAGCAAAAA ACATTTTGGT CCATTCATTG
    AATAAGTTTA AATATGGAAA AATTTCTTCC AAACATAATG GGAGTGTGAA GAAGGGTGTT
    ATTTTTGCTA CCTATTCTTC CCTTATTGGT GAAAGTCAGT CTGGTGGTAA ATATAAAACG
    AGGTTAAAAC AACTTCTACA TTGGTGCGGT GATGACTTCG ATGGAGTGAT AGTTTTTGAT
    GAATGTCATA AAGCAAAAAA CTTATGTCCT GTTGGTTCTT CAAAGCCAAC CAAGACAGGC
    TTAGCAGTTT TAGAACTTCA GAACAAATTG CCAAAAGCTA GAGTTGTCTA TGCTAGTGCC
    ACTGGTGCTT CTGAACCACG AAATATGGCC TATATGAACC GTCTTGGAAT ATGGGGTGAG
    GGAACTCCAT TTAGAGAATT CAGTGACTTT ATTCAGGCAG TAGAACGGAG AGGTGTTGGT
    GCCATGGAAA TAGTTGCTAT GGATATGAAG CTTAGAGGAA TGTACATTGC TCGACAGCTG
    AGCTTTACTG GAGTGACCTT CAAAATTGAG GAAGTTCTTC TTTCTCAGAG CTATGTTAAA
    ATGTATAACA AAGCAGTCAA ACTGTGGGTC ATTGCTCGAG AGCGGTTTCA GCAAGCTGCA
    GATCTGATTG ATGCTGAGCA GAGAATGAAG AAATCCATGT GGGGTCAGTT CTGGTCTGCT
    CACCAGAGAT TTTTCAAATA CTTGTGCATA GCGTCCAAAG TTAAAAGAGT TGTGCAACTA
    GCTCGAGAAG AAATCAAGAA TGGAAAATGT GTTGTAATTG GTCTGCAGTC TACGGGAGAA
    GCTAGAACAT TAGAAGCCTT GGAAGAGGGC GGGGGAGAAT TGAATGATTT TGTTTCAACT
    GCCAAAGGAG TGTTACAGTC ACTCATTGAA AAACACTTCC CTGCTCCGGA TAGAAAAAAG
    CTTTATAGTT TGCTGGGAAT TGATTTGACA GCTCCAAGTA ACAGCAGTTC ACCAAGAGAT
    AGTCCTTGTA AGGAAAATAA AACAAAGAAG CGGAAAGGTG AAGAAATAAC TCGAGAAGCC
    AAAAAAGCAC GAAAAATAGG TGGCCTTACT GGTAGCAGTT CTGATGATAG TGGAAGTGAA
    TCTGATGCCT CTGATAATGA AGAAAGTGAC TATGAGAGCT CTAAAAACAT GAGTTCTGGA
    GATGATGATG ATTTCAACCC ATTCAGAGAT GAGTCTAGTG AGGATGATGA AGATGATCCC
    TGGTTAATCA GAAAAGATCA TAAGAAAAAC AAAGATAAAA AAAAAAGGAA AAGTATAGAT
    CCAGATTCTA TTCAAAGTGC CTTATTAGCG TCAGGTCTTG GATCAAAACG ACCTAGTTTT
    TCTTCTACAC CAGTTATTTC ACCTGCTCCT AACAGTGCAC CAGCTAACAG TAACACCAAC
    AGCAACAGTA GCCTTATAAC AAGTCAAGAT GCCGTGGAAA GGGCCCAACA GATGAAGAAA
    GACCTACTTG ATAAACTAGA AAAATTAGCT GAAGACCTCC CTCCTAATAC CCTGGATGAA
    CTTATTGATG AACTTGGTGG CCCTGAAAAT GTTGCTGAGA TGACTGGCCG CAAGGGGAGG
    GTCGTAAGCA ATGATGACGG AAGCATATCT TATGAGTCAA GATCTGAACT TGATGTTCCT
    GTGGAAATAC TAAATATTAC AGAAAAACAA AGATTTATGG ATGGAGATAA GAATATTGCT
    ATCATCTCAG AAGCTGCCAG CTCAGGTATT TCATTACAAG CGGATAGAAG GGCTAAAAAT
    CAAAGGCGAA GAGTTCATAT GACTCTGGAG TTACCATGGA GTGCTGATAG AGCAATTCAG
    CAATTTGGAC GAACTCATAG ATCAAATCAA GTTACCGCTC CTGAATATGT CTTTCTGATT
    TCTGAATTGG CAGGAGAACA AAGATTTGCA TCTATTGTTG CTAAAAGACT TGAGAGTTTG
    GGCGCACTTA CACATGGAGA CAGAAGAGCA ACAGAATCTA GAGATCTGAG CCGGTTCAAC
    TTCGATAATA AGTATGGAAG AAATGCTTTA GAAATTGTCA TGAAATCCAT TGTAAACCTA
    GATTCTCCTA TGGTATCACC ACCTCCAGAC TATCCTGGAG AATTCTTTAA AGATGTTCGA
    CAAGGACTGA TAGGAGTTGG TCTAATAAAT GTAGAAGATC GTTCAGGAAT TCTTACTCTA
    GATAAAGATT ATAACAACAT AGGAAAATTC TTAAACAGAA TTTTGGGCAT GGAAGTGCAT
    CAACAGAATG CACTATTTCA GTATTTCGCA GACACACTTA CTGCAGTCGT TCAAAATGCC
    AAAAAAAATG GAAGATACGA TATGGGAATC TTAGATCTTG GTTCTGGGGA TGAAAAAGTG
    AGGAAAAGTG ATGTTAAGAA GTTTCTGACT CCAGGATATT CAACATCTGG CCATGTAGAA
    TTATACACAA TAAGTGTGGA GAGGGGAATG TCCTGGGAGG AAGCTACCAA GATTTGGGCT
    GAGCTGACAG GACCAGATGA TGGCTTTTAT TTGTCATTGC AAATAAGAAA CAACAAGAAA
    ACTGCCATCT TAGTTAAAGA AGTAAACCCT AAAAAGAAGC TTTTCTTAGT TTATAGACCA
    AATACCGGGA AACAACTCAA ATTAGAAATT TATGCTGATC TAAAAAAGAA ATATAAGAAG
    GTAGTCTCAG ACGATGCCGT GGTCCACTGG TTAGATCAGT ATAATTCATC TGCAGATACT
    TGTACTCATG CTTATTGGCG TGGCAACTGC AAAAAAGCAA GCTTGGGATT AGTTTGTGAA
    ATAGGTCTTC GTTGTCGCAC ATACTATGTA TTATGTGGCT CAGTACTGAG TGTCTGGACA
    AAAGTTGAGG GTGTTCTTGC GTCTGTCAGT GGCACAAATG TGAAAATGCA GATAGTTCGC
    CTAAGAACAG AGGATGGACA GCGGATTGTA GGTTTGATCA TTCCTGCAAA TTGTGTGTCT
    CCTCTTATAA ATCTCCTTTC AACTTCAGAC CAGTCTCAAC AGCTTGCAGT CCAGCAGAAA
    CAGCTCTGGC AGCAGCGTCA CCCCCAGAGC ATTGCCAGCT TGAGCAGCAC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.