ASH1L cDNA ORF clone, Bos taurus(cattle)

The following ASH1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASH1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa54718 XM_024989641.1
Latest version!
Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa17335 NM_001192743.1
Latest version!
Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa17335 XM_010802923.3
Latest version!
Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa17335 XM_010802922.2
Latest version!
Bos taurus ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa17335 XM_015462302.2
Latest version!
Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa17335 XM_024989639.1
Latest version!
Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa17335 XM_010802921.3
Latest version!
Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa232946 XM_024989640.1
Latest version!
Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa54716 XM_010802924.3
Latest version!
Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa54717 XM_010802925.2
Latest version!
Bos taurus ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OBa17335 XM_010802923.2 Bos taurus ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa17335 XM_010802921.2 Bos taurus ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa54716 XM_010802924.2 Bos taurus ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa17335 XM_015462302.1 Bos taurus ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa54718
    Clone ID Related Accession (Same CDS sequence) XM_024989641.1
    Accession Version XM_024989641.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase ASH1L isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT 
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024845409.1
    CDS302..4039
    Translation

    Target ORF information:

    RefSeq Version XM_024989641.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024989641.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT 
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17335
    Clone ID Related Accession (Same CDS sequence) XM_010802922.2 , XM_010802923.3 , XM_010802921.2 , XM_010802923.2 , XM_010802921.3 , NM_001192743.1 , XM_015462302.1 , XM_024989639.1 , XM_015462302.2
    Accession Version NM_001192743.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase ASH1L
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000003.1. On Jul 17, 2010 this sequence version replaced XM_612098.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179672.1
    CDS1..8898
    Translation

    Target ORF information:

    RefSeq Version NM_001192743.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192743.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17335
    Clone ID Related Accession (Same CDS sequence) XM_010802922.2 , XM_010802923.3 , XM_010802921.2 , XM_010802923.2 , XM_010802921.3 , NM_001192743.1 , XM_015462302.1 , XM_024989639.1 , XM_015462302.2
    Accession Version XM_010802922.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase ASH1L isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103861.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801224.1
    CDS850..9747
    Translation

    Target ORF information:

    RefSeq Version XM_010802922.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802922.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17335
    Clone ID Related Accession (Same CDS sequence) XM_010802922.2 , XM_010802923.3 , XM_010802921.2 , XM_010802923.2 , XM_010802921.3 , NM_001192743.1 , XM_015462302.1 , XM_024989639.1 , XM_015462302.2
    Accession Version XM_010802923.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase ASH1L isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802923.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
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    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801225.1
    CDS176..9073
    Translation

    Target ORF information:

    RefSeq Version XM_010802923.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802923.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17335
    Clone ID Related Accession (Same CDS sequence) XM_010802922.2 , XM_010802923.3 , XM_010802921.2 , XM_010802923.2 , XM_010802921.3 , NM_001192743.1 , XM_015462302.1 , XM_024989639.1 , XM_015462302.2
    Accession Version XM_010802921.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase ASH1L isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103861.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802921.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801223.1
    CDS187..9084
    Translation

    Target ORF information:

    RefSeq Version XM_010802921.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802921.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17335
    Clone ID Related Accession (Same CDS sequence) XM_010802922.2 , XM_010802923.3 , XM_010802921.2 , XM_010802923.2 , XM_010802921.3 , NM_001192743.1 , XM_015462302.1 , XM_024989639.1 , XM_015462302.2
    Accession Version XM_010802923.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase ASH1L isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103861.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802923.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4621
    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
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    5521
    5581
    5641
    5701
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    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801225.1
    CDS167..9064
    Translation

    Target ORF information:

    RefSeq Version XM_010802923.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802923.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17335
    Clone ID Related Accession (Same CDS sequence) XM_010802922.2 , XM_010802923.3 , XM_010802921.2 , XM_010802923.2 , XM_010802921.3 , NM_001192743.1 , XM_015462302.1 , XM_024989639.1 , XM_015462302.2
    Accession Version XM_010802921.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase ASH1L isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802921.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801223.1
    CDS225..9122
    Translation

    Target ORF information:

    RefSeq Version XM_010802921.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802921.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17335
    Clone ID Related Accession (Same CDS sequence) XM_010802922.2 , XM_010802923.3 , XM_010802921.2 , XM_010802923.2 , XM_010802921.3 , NM_001192743.1 , XM_015462302.1 , XM_024989639.1 , XM_015462302.2
    Accession Version XM_015462302.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase ASH1L isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103861.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    541
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
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    2641
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    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317788.1
    CDS357..9254
    Translation

    Target ORF information:

    RefSeq Version XM_015462302.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462302.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17335
    Clone ID Related Accession (Same CDS sequence) XM_010802922.2 , XM_010802923.3 , XM_010802921.2 , XM_010802923.2 , XM_010802921.3 , NM_001192743.1 , XM_015462302.1 , XM_024989639.1 , XM_015462302.2
    Accession Version XM_024989639.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase ASH1L isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024845407.1
    CDS231..9128
    Translation

    Target ORF information:

    RefSeq Version XM_024989639.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024989639.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17335
    Clone ID Related Accession (Same CDS sequence) XM_010802922.2 , XM_010802923.3 , XM_010802921.2 , XM_010802923.2 , XM_010802921.3 , NM_001192743.1 , XM_015462302.1 , XM_024989639.1 , XM_015462302.2
    Accession Version XM_015462302.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase ASH1L isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015462302.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317788.1
    CDS406..9303
    Translation

    Target ORF information:

    RefSeq Version XM_015462302.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462302.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232946
    Clone ID Related Accession (Same CDS sequence) XM_024989640.1
    Accession Version XM_024989640.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase ASH1L isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    181
    241
    301
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    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGACAGATT ACATTAATGC AACATCATCT ACTCTTGTTG GTAGCCGGGA TCCTGATTTA 
    AAGGACAGGG CATTACTTAA TGGAGGAACA AGCGTAACGG AAAAGTTGGC ACAGCTCATT
    GCAACTTGTC CTCCCTCCAA GTCTTCCAAG ACAAAACCGA AGAAGTTAGC AACTGGCACT
    ACTGCAGGAT TGGTTAGCAA GGATTTGATC AGGAAAGCAG GTGTTGGCTC TGTAGCTGGA
    ATAATACATA AAGACTTAAT AAAAAAACCA ACTATCAGCA CAGCGGTTGG GTTGGTAACT
    AAAGATCCTG GGAAAAAGCC AGTGTTTAAT GCAACAGTAG GATTGGTCAA TAAGGACTCT
    GTAAAAAAAC TAGGAACTGG CACTACAACG GTATTCATTA ATAAAGACTT AGGCAAAAAG
    CCAGGGGCTA TCACTACAGT AGGACTGCTG AGCAAAGATA CAGGAAAGAA GCTAGGAATT
    GGTATTGTTC CAGGTTTAGT GAATAAGGAA CCTGGGAAGA AACTAGGACT TGGCACTGTG
    GTTGGACTAG TTAATAAAGA CTTGGGAAAG AAATTGGGTT CAACTGTTGG CCTGGTAGCC
    AAGGACTGTG CAAAGAAGAT TGTAGCAAAT TCAACAGTGG GATTAGTTAA TAAGGACATT
    GGAAAGAAAC TAGTGAGTTG TCCTATGACA GGATTGGTCA ATAAAGATGC CATAAACCTT
    AAAGCGGAAG CTCTGCTCCC CACTCAGGAA CCACTTAAGG CTTCTTGTAG TACAAACATC
    ATTAGTCATG AAAGTCAGGA CCTTTCTGAA TCCCTGAAAG ACGGTGCCAC CAGCAAAACT
    TTTGAGAAGA ATGTCACACG GCAGAGTAAA GAAAGCATAT TGGAAAAGTT CTCAGTTCGA
    AAGGAAATCA TTAATTTGGA GAAAGAAATG TTTAATGAAG GAACATGTAT TCAGCAAGAC
    AGTTTCTCAT CCAGTGAAAG GGGGTCTTAT GAAACCTCAA AGCATGAAAA GCAACCTCCT
    GTATATTGCA CTTCTCCAGA CTTTCAAATG GGAGTTGCTT CTGATGCATC TACAGCAAAA
    TCCCCTTTTA GTGCAGTAGG AGAAAGCAAT CTCCCTTCCC CATCACCTAC TGTATCTGTT
    AATCCTTTAT CCAGAAGTCC CCCTGAAACT TCTTCACAGA TGACTCCTAA TCCATTACTT
    TTAAGTCCTA CCACAGAACT AATGGAAGAA ATTTCTGAAT CTGTTGGAAA GAATCAATTT
    ACTTCTGAAA GTACACATTT GAACATTGGT CATAGGTCTA TGGGTCATAG CATGAATATT
    GAATGTAAAG GGATTGATAA AGAGCTAAAT GATTCTAAAA CTACACATAT AGATATTTCA
    AGAATAAACT CTTCTCTGGG AAAAAAGCCA AGTTTGACTT CTGAATCCAG TATTCATACA
    ATTACCCCTT CAGTTGTTAA CTTCACTAGT TTATTTAGTA ACAAGCCCTT TCTGAAACTT
    GGTGCAGTAT CTGCATCTGA CAAACACTGC CAAGTTGCTG AAAGCCTAAG CACTACTTTG
    CAGTCCAAAC CCTTAAAAAA AAGGAAAGGA AGAAAACCTC GGTGGACTAA AGTGGTGGCA
    AGAAGCACAT GCCGGTCTCC AAAAGGGCTA GAATTAGAAA GATCAGAGCT TTTTAAAAAT
    GTTACGTGTA GTTCACTATC AAATAGTAAT TCCGAGCCAG CAAAGTTCAT GAAAAACATT
    GGACCATCTT CATTTGTAGA TCATGATTTC CTTAAACGCC GATTGCCAAA GTTGAGCAAA
    TCTACAGCTC CTTCTCTTGC TCTCTTAACT GATAGTGAAA AACCATCTCA TAAGTCTTTT
    GCTACTCACA AACTGTCCTC CAGTATGTGT GTCTCTAGTG ACCTTTTGTC TGATATTTAT
    AAGCCCAAAA GAGGAAGACC TAAGTCTAAG GAGATGCCTC AACTGGAGGG CCCACCTAAG
    AGGACTTTAA AAATTCCTGC TTCCAAAGTC TTTTCTTTGC AGTCTAAGGA AGAACAAGAA
    CCCCCAATTT TACAGCCGGA AATTGAGATT CCTTCTTTCA AACAAAGTCT GTCTGTGTCT
    CCTTTTCCAA AAAAGAGAGG CAGACCCAAG AGGCAAATGA GGTCACCAGT CAAGATGAAG
    CCACCTGTAC TATCGGTGGC TCCATTTGTT GCCACTGAAA GTCCAAGCAA GCTTGAATCT
    GAAAGTGATA ACCATAGAAG TAGCAGTGAT TTCTTTGAGA GTGAGGATCA ACTTCAAGAT
    CCAGATGATC TAGATGACAG TCATAGGCCA ACTGTCTGTA GTATGAGTGA CCTTGAGATG
    GAACCAGATA AAAAAATTAC CAAGAGAAAC AATGGACAAT TAATGAAAAC AATTATCCGC
    AAAATAAATA AAATGAAGAC TTTAAAGAGA AAGAAACTGT TGAATCAAAT TCTTTCAAGC
    TCTGTAGAAT CAAGTAATAA AGGGAAAGTG CAATCCAAAC TCCATAATAC AGTGTCAAGT
    CTTGCCGCTA CATTTGGCTC TAAATTGGGC CAACAGATAA ATGTCAGCAA GAAAGGAACC
    ATTTACATAG GAAAGAGGAG AGGTCGAAAA CCAAAAACTG TCTTAAATGG CATTCTTTCT
    GGTAGCCCTA CCAGCCTTGC TGTTCTTGAA CAAACAGCTC AGCAGGCAGC TGGATCGGCA
    TTAGGACAGA TTCTTCCCCC TTTGCTGCCT TCATCTGCTA GTAGTTCTGA GATTCTTCCA
    TCACCTATTT GCTCTCAGTC TTCTGGGACT AGTGGAGGTC AGAGCCCTGT AAGTAGTGAT
    GCAGGCTTTG TTGAACCCAG TTCAGTGCCA TATTTGCATT TACACTCCAG ACAGGGCAGT
    ATGATTCAGA CTCTTGCAAT GAAGAAGGCC TCAAAGGGGA GGAGGCGGTT ATCTCCTCCT
    ACTTTGTTGC CAAATTCTCC TTCACACTTG AGTGAACTCA CATCTCTGAA AGAAGCTACT
    CCTTCGCCTA TTAGTGAATC TCATAGTGAT GAGACCATTC CCAGTGATAG TGGAATTGGA
    ACAGATAATA ACAGCACATC AGACCGGGCA GAGAAATTTT GTGGGCAAAA AAAGAGGAGG
    CATTCTTTTG AGCATGTTTC TCTGATTCCC CCTGAAACCT CTACAGTCCT AAGCAGTCTT
    AAAGAAAAAC ATAAACATAA ATGTAAGCGC AGGAATCATG ATTACCTCAG CTATGACAAG
    ATGAAAAGGC AAAAACGAAA ACGGAAAAAG AAATACCCCC AACTCCGAAA TAGACAGGAT
    CCAGACTTCA TTGCAGATCT GGAGGAATTA ATAAGTCGCC TAAGTGAAAT TCGAATCACT
    CATCGAAGTC ATCATTTTAT TCCCCGAGAC CTCCTTCCAA CTATTTTTCG GATCAACTTT
    AATAGTTTCT ATACACATCC TTCTTTCCCC CTAGACCCTT TGCACTACAT TCGAAAACCT
    GACTTAAAAA AGAAGAGAGG GAGACCCCCT AAGATGAGGG AGGCAATGGC TGAAATGCCT
    TTTATGCACA GCCTTAGTTT TCCTCTCTCT AGTACTGGAT TCTATCCTTC TTACGGCATG
    CCTTACTCTC CCTCACCCCT TACAGCTGCT CCCATAGGAT TAGGTTACTA TGGAAGGTAT
    CCCCCCACTC TTTATCCACC TCCTCCATCG CCTTCTTTCA CCACTCCACT TCCACCTCCT
    TCCTATATGC ATGCTGGCCA TTTACTTCTC AATCCCACCA AATACCATAA GAAAAAGCAT
    AAGTTACTTC GACAGGAGGC CTTTCTTACA ACCAGCAGGA CTCCCCTCCT TTCCATGAGT
    ACTTACCCTA GCGTCCCTCC TGAGATGGCC TATGGTTGGA TGGTTGAGCA CAAACACAGG
    CACCGGCACA AACACAGAGA ACATCGTTCT TCTGAGCAAC CGCAGGTTTC CATGGACGCT
    GGCTCTTCCA GATCCGTCCT AGAATCTTTG AAGCGCTATC GATTTGGAAA GGACACTGTT
    GGAGAGCGAT ATAAACATAA GGAAAAGCAC CGGTGTCATA TGTCCTGCCC TCATCTCTCT
    CCTTCAAAAA GTTTAATAAA CAGAGAAGAA CAGTGGGTGC ACCGAGAGCC TTCAGAATCT
    AGTCCATTGG CCTTGGGATT GCAGACACCT TTACAGATTG ACTGTTCAGA AAGTTCTCCG
    AGTTTATCCC TTGGAGGATT CACTCCCAAC TCTGATCCAG CCAGCAGTGA TGAACATACA
    AACCTTTTCA CAAGTGCAAT AGGGAGCTGC AGAGTTTCAA ACCCTAACTC CAGTGGCCGG
    AAGAAATTAA CTGACAGCCC TGGACTGTTT TCTGCACAGG ACACTTCACT AAATCGGCCT
    CACAGAAAGG AACCACTGCC TTCCAGCGAA AGGGCAGTAC AGACCTTAAC AGGTTCCCAG
    CCAGCCTCAG ATAAGTCTTC CCAGCGACCA TCAGAGAGCA CAAACTGTAG CCCTGCACGA
    AAGAGGTCTT CATCTGAGAG TACTTCTTCA ACAGTGAATG GAGTTCCCTC TCGAAGTCCA
    AGATTGGTTT CTTCTGGGGA TGACTCTGTG GATAGTCTGC TGCAGCGAAT GGTACAACAT
    GAGGACCAAG AGCCCTTGGA GAAAAATATT GATGCTGTGA TTGCATCTGC TTCTGTGCCA
    CCTTCCTCCA GTCCAGTCCA CAGCCTCAGC AAGGAGCGAA CCCTAGGAAA ACCAGACAGC
    CTTCTAGTGC CTGCAGTCCC CAGTGACTCT TGCAGTAGTA GCATCTCACT CCTTTCTGAA
    AAGTTGCCAA GCAGCCATTC CCCCCATCAT ATTAAAAGAA GTGTAGTAGA AGCTATGCAG
    CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC
    CATGTCAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGAC AGGCCAGGAC AGGGAATAAC
    TTTGTTAAAC GCAGACCAGG TCGACCTCGG AAATGTCCTC TCCAGGCTGT GGTATCAATG
    CAAGCATTCC AAGCTGCCCA ATTTGTTGGT TCAGAATTAA ATGAAGGCGA AGAAGGAACA
    GCACTGCACC TTGGTCCTGA TACAGTTACA GATGTTATTG AAGCTGTTGT TCAGAGTGTA
    AACCTGAATC CAGACCATAA AAAGGGATTG AAGAGGAAAA GTTGGCTATT GGAAGAACAG
    ACCAAGAAAA AGCAGAAGCC ATTCCCAGAG GAAGAAGAAG AAGAGGAGAA TGCTAAAAGC
    TTTACTGAAG CAGCAGTTGA GATTCCCAGT CCTCCTGAAA CCCCAGCCAA ACCTCCCGAA
    CCTGAAAGTA CCTTGCAGCC TGTGCTTTCT CTCATCCCAA GAGAAAAGAA GGCCCCACGT
    CCCCCAAAGA AGAAGTATCA GAAAGCAGGG CTGTATTCTG ATGTTTACAA AACTACAGAC
    CCAAAGAGCC GATTGATCCA ATTAAAGAAA GAGAAGCTGG AATACACTCC AGGAGAACAT
    GAATATGGAT TATTCCCAGC CCCCATTCAT GTTGGAAAAT ATCTAAGGCA AAAGAGAATT
    GATTTTCAGC TTCCCTATGA CATTCTTTGG CAGTGGAAAC ATAATCAGCT GTACAAGAAA
    CCAGATGTCC CACTATATAA AAAAATTCGT TCAAATGTCT ACGTTGATGT CAAACCCCTT
    TCTGGTTATG AGGCTACCAC TTGTAACTGT AAGAAGCCAG ATGATGACAC CAAAAAGGGC
    TGTGTGGATG ACTGCCTCAA TAGAATGATC TTTGCTGAGT GTTCCCCCAA CACTTGCCCC
    TGTGGTGAGC AGTGCTGTAA CCAGAGGATA CAGCGGCATG AGTGGGTGCA ATGTCTAGAA
    CGATTTCGAG CTGAAGAAAA AGGTTGGGGA ATCAGAACCA AAGAACCCCT AAAAGCTGGG
    CAGTTCATCA TTGAATACCT AGGAGAGGTT GTCAGCGAGC AGGAGTTCAG GAACAGGATG
    ATTGAGCAGT ATCATAATCA TAGTGACCAC TATTGTCTGA ACTTGGATAG TGGGATGGTG
    ATTGACAGTT ACCGTATGGG AAATGAGGCC CGATTCATCA ATCACAGCTG TGACCCAAAT
    TGTGAAATGC AGAAATGGTC TGTTAATGGA GTGTACCGGA TTGGACTATA TGCTCTTAAA
    GATATGCCAG CTGGTACTGA ACTCACTTAT GATTACAACT TTCATTCCTT CAACGTTGAA
    AAACAGCAAC TGTGTAAGTG CGGCTTTGAG AAATGTCGAG GAATCATTGG GGGCAAGAGT
    CAACGCATGA ATGGACTCAC CAGCAACAAA AGTAGCCAAC CAGTGACCTC ACATAAAAAG
    TCTGGACGGT CAAAAGAGAA AAGGAAGTCT AAGCACAAGC TGAAGAAAAG GAGAGGCCAC
    CTCTCTGAGG AACCCAGTGA AAACATCAAC ACTCCAACAA GGTTGACCCC TCAATTACAA
    ATGAAGCCAA TGTCCAATCG GGAGAGAAAC TTTGTATTAA AGCATCATGT GTTCCTGGTC
    CGAAACTGGG AGAAGATTCG GCAGAAACAG GAGGAAGTAA AGCACACCAG TGATAATATT
    CATTCAGCAT CATTATATAC CCGTTGGAAT GGCATCTGCC GAGATGATGG GAACATCAAG
    TCTGATGTCT TCATGACCCA GTTCTCTGCC CTGCAGACTG CTCGATCTGT TCGAACAAGA
    CGGTTAGCAG CAGCAGAGGA AAACATTGAA GTAGCTCGGG CAGCTCGCCT AGCCCAGATC
    TTCAAAGAGA TCTGTGATGG CATAATCTCT TATAAAGATT CTTCCCGACA ATCACTAGCA
    GCACCACTTT TGAACCTACC CCCAAAGAAA AAGAATGCTG ATTATTATGA GAAGATCTCT
    GATCCCCTAG ATCTTTCCAC CATAGAGAAG CAGATCCTCA TTGGTTATTA TAAGACAGTG
    GAGGCTTTTG ATGCTGACAT GCTCAAGGTC TTTCGGAATG CAGAGAAGTA CTACGGGCGT
    AAATCCCCAG TTGGAAGAGA TGTTTGCCGC CTACGAAAGG CCTATTACAA TGCTCGGCAT
    GAGGCATCAG CCCAAATTGA TGAGATTGTG GGAGAGACAG CAAGTGAGGC AGACAGCAGT
    GAGACCTCAG TCTCAGAGAA GGAGAATGGG CATGAGAAAG ACGATGATGT TATCCGCTGT
    ATCTGTGGCC TCTACAAGGA TGAAGGCCTC ATGATCCAGT GTGACAAGTG CATGGTGTGG
    CAGCACTGTG ACTGTATGGG AGTGAACTCA GATGTGGAGC ACTACCTTTG TGAGCAGTGT
    GACCCGAGGC CTGTGGACAG AGAGGTACCC ATGATCCCTC GGCCCCACTA CACGCAACCT
    GGTTGTGTCT ACTTCATCTG TTTGCTCCGA GATGACCTGC TGCTTCGTCA AGGTGACTGT
    GTATATCTGA TGAGGGATAG TCGGCGCACC CCTGATGGCC ACCCTGTCCG TCAGTCCTAT
    CGACTGTTAT CTCACATTAA TCGAGATAAA CTTGACATAT TCCGCATTGA GAAGCTTTGG
    AAAAATGAAA AAGAGGAACG GTTTGCCTTT GGTCACCATT ATTTTCGTCC CCATGAAACC
    CACCACTCTC CATCCCGTCG GTTTTATCAC AATGAACTCT TTCGAGTGCC ACTTTATGAG
    ATCATTCCCT TGGAGGCTGT AGTGGGGACC TGCTGTGTAC TGGATCTTTA TACATACTGT
    AAAGGGAGAC CCAAAGGAGT GAAGGAGCAA GATGTGTACA TCTGTGATTA TCGGCTAGAC
    AAGTCAGCAC ACCTGTTTTA CAAGATCCAC CGGAACCGCT ATCCTGTCTG CACCAAACCC
    TATGCCTTTG ATCACTTCCC CAAGAAGCTC ACTCCAAAAA GAGATTTCTC ACCTCATTAT
    GTCCCAGACA ACTACAAGAG GAACGGAGGG CGATCATCCT GGAAGTCTGA GCGCTCAAAG
    CCACCCCTGA AAGACCTGGG CCAGGAGGAT GATGCTCTGC CCTTGATTGA AGAGGTTCTA
    GCCAGTCAGG AGCAAGCAGC CAATGAGATG CCCAGCCTGG AGGAGCCAGA ACGGGAGGGA
    GCCACTGCTG AAAACAGTGA GAGTGAAAAA AAAAAAGAGG AAAGTAGTCA AGAACCCCAA
    GTAGCCTGTA CCCCTGAGGA GCGACGGCAT AATCAACGGG AACGACTCAA CCAGATCTTG
    CTCAATCTCC TTGAAAAAAT CCCTGGGAAA AATGCCATCG ATGTGACCTA CTTGCTGGAA
    GAAGGATCAG GCAGAAAACT TCGAAGGCGT ACTTTGTTTA TCCCAGAAAA CAGCTTTCGG
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024845408.1
    CDS250..8595
    Translation

    Target ORF information:

    RefSeq Version XM_024989640.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024989640.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGACAGATT ACATTAATGC AACATCATCT ACTCTTGTTG GTAGCCGGGA TCCTGATTTA 
    AAGGACAGGG CATTACTTAA TGGAGGAACA AGCGTAACGG AAAAGTTGGC ACAGCTCATT
    GCAACTTGTC CTCCCTCCAA GTCTTCCAAG ACAAAACCGA AGAAGTTAGC AACTGGCACT
    ACTGCAGGAT TGGTTAGCAA GGATTTGATC AGGAAAGCAG GTGTTGGCTC TGTAGCTGGA
    ATAATACATA AAGACTTAAT AAAAAAACCA ACTATCAGCA CAGCGGTTGG GTTGGTAACT
    AAAGATCCTG GGAAAAAGCC AGTGTTTAAT GCAACAGTAG GATTGGTCAA TAAGGACTCT
    GTAAAAAAAC TAGGAACTGG CACTACAACG GTATTCATTA ATAAAGACTT AGGCAAAAAG
    CCAGGGGCTA TCACTACAGT AGGACTGCTG AGCAAAGATA CAGGAAAGAA GCTAGGAATT
    GGTATTGTTC CAGGTTTAGT GAATAAGGAA CCTGGGAAGA AACTAGGACT TGGCACTGTG
    GTTGGACTAG TTAATAAAGA CTTGGGAAAG AAATTGGGTT CAACTGTTGG CCTGGTAGCC
    AAGGACTGTG CAAAGAAGAT TGTAGCAAAT TCAACAGTGG GATTAGTTAA TAAGGACATT
    GGAAAGAAAC TAGTGAGTTG TCCTATGACA GGATTGGTCA ATAAAGATGC CATAAACCTT
    AAAGCGGAAG CTCTGCTCCC CACTCAGGAA CCACTTAAGG CTTCTTGTAG TACAAACATC
    ATTAGTCATG AAAGTCAGGA CCTTTCTGAA TCCCTGAAAG ACGGTGCCAC CAGCAAAACT
    TTTGAGAAGA ATGTCACACG GCAGAGTAAA GAAAGCATAT TGGAAAAGTT CTCAGTTCGA
    AAGGAAATCA TTAATTTGGA GAAAGAAATG TTTAATGAAG GAACATGTAT TCAGCAAGAC
    AGTTTCTCAT CCAGTGAAAG GGGGTCTTAT GAAACCTCAA AGCATGAAAA GCAACCTCCT
    GTATATTGCA CTTCTCCAGA CTTTCAAATG GGAGTTGCTT CTGATGCATC TACAGCAAAA
    TCCCCTTTTA GTGCAGTAGG AGAAAGCAAT CTCCCTTCCC CATCACCTAC TGTATCTGTT
    AATCCTTTAT CCAGAAGTCC CCCTGAAACT TCTTCACAGA TGACTCCTAA TCCATTACTT
    TTAAGTCCTA CCACAGAACT AATGGAAGAA ATTTCTGAAT CTGTTGGAAA GAATCAATTT
    ACTTCTGAAA GTACACATTT GAACATTGGT CATAGGTCTA TGGGTCATAG CATGAATATT
    GAATGTAAAG GGATTGATAA AGAGCTAAAT GATTCTAAAA CTACACATAT AGATATTTCA
    AGAATAAACT CTTCTCTGGG AAAAAAGCCA AGTTTGACTT CTGAATCCAG TATTCATACA
    ATTACCCCTT CAGTTGTTAA CTTCACTAGT TTATTTAGTA ACAAGCCCTT TCTGAAACTT
    GGTGCAGTAT CTGCATCTGA CAAACACTGC CAAGTTGCTG AAAGCCTAAG CACTACTTTG
    CAGTCCAAAC CCTTAAAAAA AAGGAAAGGA AGAAAACCTC GGTGGACTAA AGTGGTGGCA
    AGAAGCACAT GCCGGTCTCC AAAAGGGCTA GAATTAGAAA GATCAGAGCT TTTTAAAAAT
    GTTACGTGTA GTTCACTATC AAATAGTAAT TCCGAGCCAG CAAAGTTCAT GAAAAACATT
    GGACCATCTT CATTTGTAGA TCATGATTTC CTTAAACGCC GATTGCCAAA GTTGAGCAAA
    TCTACAGCTC CTTCTCTTGC TCTCTTAACT GATAGTGAAA AACCATCTCA TAAGTCTTTT
    GCTACTCACA AACTGTCCTC CAGTATGTGT GTCTCTAGTG ACCTTTTGTC TGATATTTAT
    AAGCCCAAAA GAGGAAGACC TAAGTCTAAG GAGATGCCTC AACTGGAGGG CCCACCTAAG
    AGGACTTTAA AAATTCCTGC TTCCAAAGTC TTTTCTTTGC AGTCTAAGGA AGAACAAGAA
    CCCCCAATTT TACAGCCGGA AATTGAGATT CCTTCTTTCA AACAAAGTCT GTCTGTGTCT
    CCTTTTCCAA AAAAGAGAGG CAGACCCAAG AGGCAAATGA GGTCACCAGT CAAGATGAAG
    CCACCTGTAC TATCGGTGGC TCCATTTGTT GCCACTGAAA GTCCAAGCAA GCTTGAATCT
    GAAAGTGATA ACCATAGAAG TAGCAGTGAT TTCTTTGAGA GTGAGGATCA ACTTCAAGAT
    CCAGATGATC TAGATGACAG TCATAGGCCA ACTGTCTGTA GTATGAGTGA CCTTGAGATG
    GAACCAGATA AAAAAATTAC CAAGAGAAAC AATGGACAAT TAATGAAAAC AATTATCCGC
    AAAATAAATA AAATGAAGAC TTTAAAGAGA AAGAAACTGT TGAATCAAAT TCTTTCAAGC
    TCTGTAGAAT CAAGTAATAA AGGGAAAGTG CAATCCAAAC TCCATAATAC AGTGTCAAGT
    CTTGCCGCTA CATTTGGCTC TAAATTGGGC CAACAGATAA ATGTCAGCAA GAAAGGAACC
    ATTTACATAG GAAAGAGGAG AGGTCGAAAA CCAAAAACTG TCTTAAATGG CATTCTTTCT
    GGTAGCCCTA CCAGCCTTGC TGTTCTTGAA CAAACAGCTC AGCAGGCAGC TGGATCGGCA
    TTAGGACAGA TTCTTCCCCC TTTGCTGCCT TCATCTGCTA GTAGTTCTGA GATTCTTCCA
    TCACCTATTT GCTCTCAGTC TTCTGGGACT AGTGGAGGTC AGAGCCCTGT AAGTAGTGAT
    GCAGGCTTTG TTGAACCCAG TTCAGTGCCA TATTTGCATT TACACTCCAG ACAGGGCAGT
    ATGATTCAGA CTCTTGCAAT GAAGAAGGCC TCAAAGGGGA GGAGGCGGTT ATCTCCTCCT
    ACTTTGTTGC CAAATTCTCC TTCACACTTG AGTGAACTCA CATCTCTGAA AGAAGCTACT
    CCTTCGCCTA TTAGTGAATC TCATAGTGAT GAGACCATTC CCAGTGATAG TGGAATTGGA
    ACAGATAATA ACAGCACATC AGACCGGGCA GAGAAATTTT GTGGGCAAAA AAAGAGGAGG
    CATTCTTTTG AGCATGTTTC TCTGATTCCC CCTGAAACCT CTACAGTCCT AAGCAGTCTT
    AAAGAAAAAC ATAAACATAA ATGTAAGCGC AGGAATCATG ATTACCTCAG CTATGACAAG
    ATGAAAAGGC AAAAACGAAA ACGGAAAAAG AAATACCCCC AACTCCGAAA TAGACAGGAT
    CCAGACTTCA TTGCAGATCT GGAGGAATTA ATAAGTCGCC TAAGTGAAAT TCGAATCACT
    CATCGAAGTC ATCATTTTAT TCCCCGAGAC CTCCTTCCAA CTATTTTTCG GATCAACTTT
    AATAGTTTCT ATACACATCC TTCTTTCCCC CTAGACCCTT TGCACTACAT TCGAAAACCT
    GACTTAAAAA AGAAGAGAGG GAGACCCCCT AAGATGAGGG AGGCAATGGC TGAAATGCCT
    TTTATGCACA GCCTTAGTTT TCCTCTCTCT AGTACTGGAT TCTATCCTTC TTACGGCATG
    CCTTACTCTC CCTCACCCCT TACAGCTGCT CCCATAGGAT TAGGTTACTA TGGAAGGTAT
    CCCCCCACTC TTTATCCACC TCCTCCATCG CCTTCTTTCA CCACTCCACT TCCACCTCCT
    TCCTATATGC ATGCTGGCCA TTTACTTCTC AATCCCACCA AATACCATAA GAAAAAGCAT
    AAGTTACTTC GACAGGAGGC CTTTCTTACA ACCAGCAGGA CTCCCCTCCT TTCCATGAGT
    ACTTACCCTA GCGTCCCTCC TGAGATGGCC TATGGTTGGA TGGTTGAGCA CAAACACAGG
    CACCGGCACA AACACAGAGA ACATCGTTCT TCTGAGCAAC CGCAGGTTTC CATGGACGCT
    GGCTCTTCCA GATCCGTCCT AGAATCTTTG AAGCGCTATC GATTTGGAAA GGACACTGTT
    GGAGAGCGAT ATAAACATAA GGAAAAGCAC CGGTGTCATA TGTCCTGCCC TCATCTCTCT
    CCTTCAAAAA GTTTAATAAA CAGAGAAGAA CAGTGGGTGC ACCGAGAGCC TTCAGAATCT
    AGTCCATTGG CCTTGGGATT GCAGACACCT TTACAGATTG ACTGTTCAGA AAGTTCTCCG
    AGTTTATCCC TTGGAGGATT CACTCCCAAC TCTGATCCAG CCAGCAGTGA TGAACATACA
    AACCTTTTCA CAAGTGCAAT AGGGAGCTGC AGAGTTTCAA ACCCTAACTC CAGTGGCCGG
    AAGAAATTAA CTGACAGCCC TGGACTGTTT TCTGCACAGG ACACTTCACT AAATCGGCCT
    CACAGAAAGG AACCACTGCC TTCCAGCGAA AGGGCAGTAC AGACCTTAAC AGGTTCCCAG
    CCAGCCTCAG ATAAGTCTTC CCAGCGACCA TCAGAGAGCA CAAACTGTAG CCCTGCACGA
    AAGAGGTCTT CATCTGAGAG TACTTCTTCA ACAGTGAATG GAGTTCCCTC TCGAAGTCCA
    AGATTGGTTT CTTCTGGGGA TGACTCTGTG GATAGTCTGC TGCAGCGAAT GGTACAACAT
    GAGGACCAAG AGCCCTTGGA GAAAAATATT GATGCTGTGA TTGCATCTGC TTCTGTGCCA
    CCTTCCTCCA GTCCAGTCCA CAGCCTCAGC AAGGAGCGAA CCCTAGGAAA ACCAGACAGC
    CTTCTAGTGC CTGCAGTCCC CAGTGACTCT TGCAGTAGTA GCATCTCACT CCTTTCTGAA
    AAGTTGCCAA GCAGCCATTC CCCCCATCAT ATTAAAAGAA GTGTAGTAGA AGCTATGCAG
    CGCCAAGCTC GGAAAATGTG CAATTATGAC AAAATCTTGG CCACCAAGAA AAACCTGGAC
    CATGTCAATA AAATTTTAAA AGCCAAAAAA CTTCAAAGAC AGGCCAGGAC AGGGAATAAC
    TTTGTTAAAC GCAGACCAGG TCGACCTCGG AAATGTCCTC TCCAGGCTGT GGTATCAATG
    CAAGCATTCC AAGCTGCCCA ATTTGTTGGT TCAGAATTAA ATGAAGGCGA AGAAGGAACA
    GCACTGCACC TTGGTCCTGA TACAGTTACA GATGTTATTG AAGCTGTTGT TCAGAGTGTA
    AACCTGAATC CAGACCATAA AAAGGGATTG AAGAGGAAAA GTTGGCTATT GGAAGAACAG
    ACCAAGAAAA AGCAGAAGCC ATTCCCAGAG GAAGAAGAAG AAGAGGAGAA TGCTAAAAGC
    TTTACTGAAG CAGCAGTTGA GATTCCCAGT CCTCCTGAAA CCCCAGCCAA ACCTCCCGAA
    CCTGAAAGTA CCTTGCAGCC TGTGCTTTCT CTCATCCCAA GAGAAAAGAA GGCCCCACGT
    CCCCCAAAGA AGAAGTATCA GAAAGCAGGG CTGTATTCTG ATGTTTACAA AACTACAGAC
    CCAAAGAGCC GATTGATCCA ATTAAAGAAA GAGAAGCTGG AATACACTCC AGGAGAACAT
    GAATATGGAT TATTCCCAGC CCCCATTCAT GTTGGAAAAT ATCTAAGGCA AAAGAGAATT
    GATTTTCAGC TTCCCTATGA CATTCTTTGG CAGTGGAAAC ATAATCAGCT GTACAAGAAA
    CCAGATGTCC CACTATATAA AAAAATTCGT TCAAATGTCT ACGTTGATGT CAAACCCCTT
    TCTGGTTATG AGGCTACCAC TTGTAACTGT AAGAAGCCAG ATGATGACAC CAAAAAGGGC
    TGTGTGGATG ACTGCCTCAA TAGAATGATC TTTGCTGAGT GTTCCCCCAA CACTTGCCCC
    TGTGGTGAGC AGTGCTGTAA CCAGAGGATA CAGCGGCATG AGTGGGTGCA ATGTCTAGAA
    CGATTTCGAG CTGAAGAAAA AGGTTGGGGA ATCAGAACCA AAGAACCCCT AAAAGCTGGG
    CAGTTCATCA TTGAATACCT AGGAGAGGTT GTCAGCGAGC AGGAGTTCAG GAACAGGATG
    ATTGAGCAGT ATCATAATCA TAGTGACCAC TATTGTCTGA ACTTGGATAG TGGGATGGTG
    ATTGACAGTT ACCGTATGGG AAATGAGGCC CGATTCATCA ATCACAGCTG TGACCCAAAT
    TGTGAAATGC AGAAATGGTC TGTTAATGGA GTGTACCGGA TTGGACTATA TGCTCTTAAA
    GATATGCCAG CTGGTACTGA ACTCACTTAT GATTACAACT TTCATTCCTT CAACGTTGAA
    AAACAGCAAC TGTGTAAGTG CGGCTTTGAG AAATGTCGAG GAATCATTGG GGGCAAGAGT
    CAACGCATGA ATGGACTCAC CAGCAACAAA AGTAGCCAAC CAGTGACCTC ACATAAAAAG
    TCTGGACGGT CAAAAGAGAA AAGGAAGTCT AAGCACAAGC TGAAGAAAAG GAGAGGCCAC
    CTCTCTGAGG AACCCAGTGA AAACATCAAC ACTCCAACAA GGTTGACCCC TCAATTACAA
    ATGAAGCCAA TGTCCAATCG GGAGAGAAAC TTTGTATTAA AGCATCATGT GTTCCTGGTC
    CGAAACTGGG AGAAGATTCG GCAGAAACAG GAGGAAGTAA AGCACACCAG TGATAATATT
    CATTCAGCAT CATTATATAC CCGTTGGAAT GGCATCTGCC GAGATGATGG GAACATCAAG
    TCTGATGTCT TCATGACCCA GTTCTCTGCC CTGCAGACTG CTCGATCTGT TCGAACAAGA
    CGGTTAGCAG CAGCAGAGGA AAACATTGAA GTAGCTCGGG CAGCTCGCCT AGCCCAGATC
    TTCAAAGAGA TCTGTGATGG CATAATCTCT TATAAAGATT CTTCCCGACA ATCACTAGCA
    GCACCACTTT TGAACCTACC CCCAAAGAAA AAGAATGCTG ATTATTATGA GAAGATCTCT
    GATCCCCTAG ATCTTTCCAC CATAGAGAAG CAGATCCTCA TTGGTTATTA TAAGACAGTG
    GAGGCTTTTG ATGCTGACAT GCTCAAGGTC TTTCGGAATG CAGAGAAGTA CTACGGGCGT
    AAATCCCCAG TTGGAAGAGA TGTTTGCCGC CTACGAAAGG CCTATTACAA TGCTCGGCAT
    GAGGCATCAG CCCAAATTGA TGAGATTGTG GGAGAGACAG CAAGTGAGGC AGACAGCAGT
    GAGACCTCAG TCTCAGAGAA GGAGAATGGG CATGAGAAAG ACGATGATGT TATCCGCTGT
    ATCTGTGGCC TCTACAAGGA TGAAGGCCTC ATGATCCAGT GTGACAAGTG CATGGTGTGG
    CAGCACTGTG ACTGTATGGG AGTGAACTCA GATGTGGAGC ACTACCTTTG TGAGCAGTGT
    GACCCGAGGC CTGTGGACAG AGAGGTACCC ATGATCCCTC GGCCCCACTA CACGCAACCT
    GGTTGTGTCT ACTTCATCTG TTTGCTCCGA GATGACCTGC TGCTTCGTCA AGGTGACTGT
    GTATATCTGA TGAGGGATAG TCGGCGCACC CCTGATGGCC ACCCTGTCCG TCAGTCCTAT
    CGACTGTTAT CTCACATTAA TCGAGATAAA CTTGACATAT TCCGCATTGA GAAGCTTTGG
    AAAAATGAAA AAGAGGAACG GTTTGCCTTT GGTCACCATT ATTTTCGTCC CCATGAAACC
    CACCACTCTC CATCCCGTCG GTTTTATCAC AATGAACTCT TTCGAGTGCC ACTTTATGAG
    ATCATTCCCT TGGAGGCTGT AGTGGGGACC TGCTGTGTAC TGGATCTTTA TACATACTGT
    AAAGGGAGAC CCAAAGGAGT GAAGGAGCAA GATGTGTACA TCTGTGATTA TCGGCTAGAC
    AAGTCAGCAC ACCTGTTTTA CAAGATCCAC CGGAACCGCT ATCCTGTCTG CACCAAACCC
    TATGCCTTTG ATCACTTCCC CAAGAAGCTC ACTCCAAAAA GAGATTTCTC ACCTCATTAT
    GTCCCAGACA ACTACAAGAG GAACGGAGGG CGATCATCCT GGAAGTCTGA GCGCTCAAAG
    CCACCCCTGA AAGACCTGGG CCAGGAGGAT GATGCTCTGC CCTTGATTGA AGAGGTTCTA
    GCCAGTCAGG AGCAAGCAGC CAATGAGATG CCCAGCCTGG AGGAGCCAGA ACGGGAGGGA
    GCCACTGCTG AAAACAGTGA GAGTGAAAAA AAAAAAGAGG AAAGTAGTCA AGAACCCCAA
    GTAGCCTGTA CCCCTGAGGA GCGACGGCAT AATCAACGGG AACGACTCAA CCAGATCTTG
    CTCAATCTCC TTGAAAAAAT CCCTGGGAAA AATGCCATCG ATGTGACCTA CTTGCTGGAA
    GAAGGATCAG GCAGAAAACT TCGAAGGCGT ACTTTGTTTA TCCCAGAAAA CAGCTTTCGG
    AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54716
    Clone ID Related Accession (Same CDS sequence) XM_010802924.3 , XM_010802924.2
    Accession Version XM_010802924.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase ASH1L isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802924.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801226.1
    CDS225..8942
    Translation

    Target ORF information:

    RefSeq Version XM_010802924.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ASH1 like histone lysine methyltransferase (ASH1L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802924.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54716
    Clone ID Related Accession (Same CDS sequence) XM_010802924.3 , XM_010802924.2
    Accession Version XM_010802924.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase ASH1L isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103861.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802924.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
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    481
    541
    601
    661
    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
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    7921
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    8401
    8461
    8521
    8581
    8641
    8701
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801226.1
    CDS187..8904
    Translation

    Target ORF information:

    RefSeq Version XM_010802924.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802924.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCACCTCATT ATGTCCCAGA CAACTACAAG AGGAACGGAG GGCGATCATC CTGGAAGTCT
    GAGCGCTCAA AGCCACCCCT GAAAGACCTG GGCCAGGAGG ATGATGCTCT GCCCTTGATT
    GAAGAGGTTC TAGCCAGTCA GGAGCAAGCA GCCAATGAGA TGCCCAGCCT GGAGGAGCCA
    GAACGGGAGG GAGCCACTGC TGAAAACAGT GAGAGTGAAA AAAAAAAAGA GGAAAGTAGT
    CAAGAACCCC AAGTAGCCTG TACCCCTGAG GAGCGACGGC ATAATCAACG GGAACGACTC
    AACCAGATCT TGCTCAATCT CCTTGAAAAA ATCCCTGGGA AAAATGCCAT CGATGTGACC
    TACTTGCTGG AAGAAGGATC AGGCAGAAAA CTTCGAAGGC GTACTTTGTT TATCCCAGAA
    AACAGCTTTC GGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54717
    Clone ID Related Accession (Same CDS sequence) XM_010802925.2
    Accession Version XM_010802925.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8481bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase ASH1L isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103861.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802925.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCAATCATCC TGGAAGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801227.1
    CDS187..8667
    Translation

    Target ORF information:

    RefSeq Version XM_010802925.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ash1 (absent, small, or homeotic)-like (Drosophila) (ASH1L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802925.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGACCCTA GAAATACTGC TATGTTAGGA TTGGGTTCTG ATTCCGAAGG ATTTTCAAGA 
    AAGAGTCCCT CTGCCATCAA TACGGGCACA CTGGTCAGCA AGAGAGAAGT AGAGCTAGAG
    AACAACACAA AGGAAGAAGA AGACCTTCGC AAGTGGAATC GGGAAAGAAA CACTGAAGCA
    GGGAAAGATA ATGGTTTGAC TGATGCACAG CAACAGTTTT CAGTGAAAGA AACAAACTTT
    ACAGAAGGAA ATTTAAAATT GAAAATTGGC CTTCAGGCTA AGAGAACTAA AAAACCTCCA
    AAGAACTTGG AGAACTATGT ATGTCGACCT GCCATAAAGA CAACTATCAA GCACCCAAGG
    AAAGCACTTA AAAGTGGGAA GATGACAGAT GAAAAGAATG AACACTGCCC TTCAAGACGG
    GATCCTTCAA AGTTGTACAA GAAATCAGGT GATGTTGCAG CCATTGAATG CCAGTCTGAA
    GAAACCATCC ATCTTCATTC ACAGGGAGAA AACAGTCCTT TGTCTAAGAA GCTTTCTCCA
    GTACACTCAG AAATGACAGA TTACATTAAT GCAACATCAT CTACTCTTGT TGGTAGCCGG
    GATCCTGATT TAAAGGACAG GGCATTACTT AATGGAGGAA CAAGCGTAAC GGAAAAGTTG
    GCACAGCTCA TTGCAACTTG TCCTCCCTCC AAGTCTTCCA AGACAAAACC GAAGAAGTTA
    GCAACTGGCA CTACTGCAGG ATTGGTTAGC AAGGATTTGA TCAGGAAAGC AGGTGTTGGC
    TCTGTAGCTG GAATAATACA TAAAGACTTA ATAAAAAAAC CAACTATCAG CACAGCGGTT
    GGGTTGGTAA CTAAAGATCC TGGGAAAAAG CCAGTGTTTA ATGCAACAGT AGGATTGGTC
    AATAAGGACT CTGTAAAAAA ACTAGGAACT GGCACTACAA CGGTATTCAT TAATAAAGAC
    TTAGGCAAAA AGCCAGGGGC TATCACTACA GTAGGACTGC TGAGCAAAGA TACAGGAAAG
    AAGCTAGGAA TTGGTATTGT TCCAGGTTTA GTGAATAAGG AACCTGGGAA GAAACTAGGA
    CTTGGCACTG TGGTTGGACT AGTTAATAAA GACTTGGGAA AGAAATTGGG TTCAACTGTT
    GGCCTGGTAG CCAAGGACTG TGCAAAGAAG ATTGTAGCAA ATTCAACAGT GGGATTAGTT
    AATAAGGACA TTGGAAAGAA ACTAGTGAGT TGTCCTATGA CAGGATTGGT CAATAAAGAT
    GCCATAAACC TTAAAGCGGA AGCTCTGCTC CCCACTCAGG AACCACTTAA GGCTTCTTGT
    AGTACAAACA TCATTAGTCA TGAAAGTCAG GACCTTTCTG AATCCCTGAA AGACGGTGCC
    ACCAGCAAAA CTTTTGAGAA GAATGTCACA CGGCAGAGTA AAGAAAGCAT ATTGGAAAAG
    TTCTCAGTTC GAAAGGAAAT CATTAATTTG GAGAAAGAAA TGTTTAATGA AGGAACATGT
    ATTCAGCAAG ACAGTTTCTC ATCCAGTGAA AGGGGGTCTT ATGAAACCTC AAAGCATGAA
    AAGCAACCTC CTGTATATTG CACTTCTCCA GACTTTCAAA TGGGAGTTGC TTCTGATGCA
    TCTACAGCAA AATCCCCTTT TAGTGCAGTA GGAGAAAGCA ATCTCCCTTC CCCATCACCT
    ACTGTATCTG TTAATCCTTT ATCCAGAAGT CCCCCTGAAA CTTCTTCACA GATGACTCCT
    AATCCATTAC TTTTAAGTCC TACCACAGAA CTAATGGAAG AAATTTCTGA ATCTGTTGGA
    AAGAATCAAT TTACTTCTGA AAGTACACAT TTGAACATTG GTCATAGGTC TATGGGTCAT
    AGCATGAATA TTGAATGTAA AGGGATTGAT AAAGAGCTAA ATGATTCTAA AACTACACAT
    ATAGATATTT CAAGAATAAA CTCTTCTCTG GGAAAAAAGC CAAGTTTGAC TTCTGAATCC
    AGTATTCATA CAATTACCCC TTCAGTTGTT AACTTCACTA GTTTATTTAG TAACAAGCCC
    TTTCTGAAAC TTGGTGCAGT ATCTGCATCT GACAAACACT GCCAAGTTGC TGAAAGCCTA
    AGCACTACTT TGCAGTCCAA ACCCTTAAAA AAAAGGAAAG GAAGAAAACC TCGGTGGACT
    AAAGTGGTGG CAAGAAGCAC ATGCCGGTCT CCAAAAGGGC TAGAATTAGA AAGATCAGAG
    CTTTTTAAAA ATGTTACGTG TAGTTCACTA TCAAATAGTA ATTCCGAGCC AGCAAAGTTC
    ATGAAAAACA TTGGACCATC TTCATTTGTA GATCATGATT TCCTTAAACG CCGATTGCCA
    AAGTTGAGCA AATCTACAGC TCCTTCTCTT GCTCTCTTAA CTGATAGTGA AAAACCATCT
    CATAAGTCTT TTGCTACTCA CAAACTGTCC TCCAGTATGT GTGTCTCTAG TGACCTTTTG
    TCTGATATTT ATAAGCCCAA AAGAGGAAGA CCTAAGTCTA AGGAGATGCC TCAACTGGAG
    GGCCCACCTA AGAGGACTTT AAAAATTCCT GCTTCCAAAG TCTTTTCTTT GCAGTCTAAG
    GAAGAACAAG AACCCCCAAT TTTACAGCCG GAAATTGAGA TTCCTTCTTT CAAACAAAGT
    CTGTCTGTGT CTCCTTTTCC AAAAAAGAGA GGCAGACCCA AGAGGCAAAT GAGGTCACCA
    GTCAAGATGA AGCCACCTGT ACTATCGGTG GCTCCATTTG TTGCCACTGA AAGTCCAAGC
    AAGCTTGAAT CTGAAAGTGA TAACCATAGA AGTAGCAGTG ATTTCTTTGA GAGTGAGGAT
    CAACTTCAAG ATCCAGATGA TCTAGATGAC AGTCATAGGC CAACTGTCTG TAGTATGAGT
    GACCTTGAGA TGGAACCAGA TAAAAAAATT ACCAAGAGAA ACAATGGACA ATTAATGAAA
    ACAATTATCC GCAAAATAAA TAAAATGAAG ACTTTAAAGA GAAAGAAACT GTTGAATCAA
    ATTCTTTCAA GCTCTGTAGA ATCAAGTAAT AAAGGGAAAG TGCAATCCAA ACTCCATAAT
    ACAGTGTCAA GTCTTGCCGC TACATTTGGC TCTAAATTGG GCCAACAGAT AAATGTCAGC
    AAGAAAGGAA CCATTTACAT AGGAAAGAGG AGAGGTCGAA AACCAAAAAC TGTCTTAAAT
    GGCATTCTTT CTGGTAGCCC TACCAGCCTT GCTGTTCTTG AACAAACAGC TCAGCAGGCA
    GCTGGATCGG CATTAGGACA GATTCTTCCC CCTTTGCTGC CTTCATCTGC TAGTAGTTCT
    GAGATTCTTC CATCACCTAT TTGCTCTCAG TCTTCTGGGA CTAGTGGAGG TCAGAGCCCT
    GTAAGTAGTG ATGCAGGCTT TGTTGAACCC AGTTCAGTGC CATATTTGCA TTTACACTCC
    AGACAGGGCA GTATGATTCA GACTCTTGCA ATGAAGAAGG CCTCAAAGGG GAGGAGGCGG
    TTATCTCCTC CTACTTTGTT GCCAAATTCT CCTTCACACT TGAGTGAACT CACATCTCTG
    AAAGAAGCTA CTCCTTCGCC TATTAGTGAA TCTCATAGTG ATGAGACCAT TCCCAGTGAT
    AGTGGAATTG GAACAGATAA TAACAGCACA TCAGACCGGG CAGAGAAATT TTGTGGGCAA
    AAAAAGAGGA GGCATTCTTT TGAGCATGTT TCTCTGATTC CCCCTGAAAC CTCTACAGTC
    CTAAGCAGTC TTAAAGAAAA ACATAAACAT AAATGTAAGC GCAGGAATCA TGATTACCTC
    AGCTATGACA AGATGAAAAG GCAAAAACGA AAACGGAAAA AGAAATACCC CCAACTCCGA
    AATAGACAGG ATCCAGACTT CATTGCAGAT CTGGAGGAAT TAATAAGTCG CCTAAGTGAA
    ATTCGAATCA CTCATCGAAG TCATCATTTT ATTCCCCGAG ACCTCCTTCC AACTATTTTT
    CGGATCAACT TTAATAGTTT CTATACACAT CCTTCTTTCC CCCTAGACCC TTTGCACTAC
    ATTCGAAAAC CTGACTTAAA AAAGAAGAGA GGGAGACCCC CTAAGATGAG GGAGGCAATG
    GCTGAAATGC CTTTTATGCA CAGCCTTAGT TTTCCTCTCT CTAGTACTGG ATTCTATCCT
    TCTTACGGCA TGCCTTACTC TCCCTCACCC CTTACAGCTG CTCCCATAGG ATTAGGTTAC
    TATGGAAGGT ATCCCCCCAC TCTTTATCCA CCTCCTCCAT CGCCTTCTTT CACCACTCCA
    CTTCCACCTC CTTCCTATAT GCATGCTGGC CATTTACTTC TCAATCCCAC CAAATACCAT
    AAGAAAAAGC ATAAGTTACT TCGACAGGAG GCCTTTCTTA CAACCAGCAG GACTCCCCTC
    CTTTCCATGA GTACTTACCC TAGCGTCCCT CCTGAGATGG CCTATGGTTG GATGGTTGAG
    CACAAACACA GGCACCGGCA CAAACACAGA GAACATCGTT CTTCTGAGCA ACCGCAGGTT
    TCCATGGACG CTGGCTCTTC CAGATCCGTC CTAGAATCTT TGAAGCGCTA TCGATTTGGA
    AAGGACACTG TTGGAGAGCG ATATAAACAT AAGGAAAAGC ACCGGTGTCA TATGTCCTGC
    CCTCATCTCT CTCCTTCAAA AAGTTTAATA AACAGAGAAG AACAGTGGGT GCACCGAGAG
    CCTTCAGAAT CTAGTCCATT GGCCTTGGGA TTGCAGACAC CTTTACAGAT TGACTGTTCA
    GAAAGTTCTC CGAGTTTATC CCTTGGAGGA TTCACTCCCA ACTCTGATCC AGCCAGCAGT
    GATGAACATA CAAACCTTTT CACAAGTGCA ATAGGGAGCT GCAGAGTTTC AAACCCTAAC
    TCCAGTGGCC GGAAGAAATT AACTGACAGC CCTGGACTGT TTTCTGCACA GGACACTTCA
    CTAAATCGGC CTCACAGAAA GGAACCACTG CCTTCCAGCG AAAGGGCAGT ACAGACCTTA
    ACAGGTTCCC AGCCAGCCTC AGATAAGTCT TCCCAGCGAC CATCAGAGAG CACAAACTGT
    AGCCCTGCAC GAAAGAGGTC TTCATCTGAG AGTACTTCTT CAACAGTGAA TGGAGTTCCC
    TCTCGAAGTC CAAGATTGGT TTCTTCTGGG GATGACTCTG TGGATAGTCT GCTGCAGCGA
    ATGGTACAAC ATGAGGACCA AGAGCCCTTG GAGAAAAATA TTGATGCTGT GATTGCATCT
    GCTTCTGTGC CACCTTCCTC CAGTCCAGTC CACAGCCTCA GCAAGGAGCG AACCCTAGGA
    AAACCAGACA GCCTTCTAGT GCCTGCAGTC CCCAGTGACT CTTGCAGTAG TAGCATCTCA
    CTCCTTTCTG AAAAGTTGCC AAGCAGCCAT TCCCCCCATC ATATTAAAAG AAGTGTAGTA
    GAAGCTATGC AGCGCCAAGC TCGGAAAATG TGCAATTATG ACAAAATCTT GGCCACCAAG
    AAAAACCTGG ACCATGTCAA TAAAATTTTA AAAGCCAAAA AACTTCAAAG ACAGGCCAGG
    ACAGGGAATA ACTTTGTTAA ACGCAGACCA GGTCGACCTC GGAAATGTCC TCTCCAGGCT
    GTGGTATCAA TGCAAGCATT CCAAGCTGCC CAATTTGTTG GTTCAGAATT AAATGAAGGC
    GAAGAAGGAA CAGCACTGCA CCTTGGTCCT GATACAGTTA CAGATGTTAT TGAAGCTGTT
    GTTCAGAGTG TAAACCTGAA TCCAGACCAT AAAAAGGGAT TGAAGAGGAA AAGTTGGCTA
    TTGGAAGAAC AGACCAAGAA AAAGCAGAAG CCATTCCCAG AGGAAGAAGA AGAAGAGGAG
    AATGCTAAAA GCTTTACTGA AGCAGCAGTT GAGATTCCCA GTCCTCCTGA AACCCCAGCC
    AAACCTCCCG AACCTGAAAG TACCTTGCAG CCTGTGCTTT CTCTCATCCC AAGAGAAAAG
    AAGGCCCCAC GTCCCCCAAA GAAGAAGTAT CAGAAAGCAG GGCTGTATTC TGATGTTTAC
    AAAACTACAG ACCCAAAGAG CCGATTGATC CAATTAAAGA AAGAGAAGCT GGAATACACT
    CCAGGAGAAC ATGAATATGG ATTATTCCCA GCCCCCATTC ATGTTGGAAA ATATCTAAGG
    CAAAAGAGAA TTGATTTTCA GCTTCCCTAT GACATTCTTT GGCAGTGGAA ACATAATCAG
    CTGTACAAGA AACCAGATGT CCCACTATAT AAAAAAATTC GTTCAAATGT CTACGTTGAT
    GTCAAACCCC TTTCTGGTTA TGAGGCTACC ACTTGTAACT GTAAGAAGCC AGATGATGAC
    ACCAAAAAGG GCTGTGTGGA TGACTGCCTC AATAGAATGA TCTTTGCTGA GTGTTCCCCC
    AACACTTGCC CCTGTGGTGA GCAGTGCTGT AACCAGAGGA TACAGCGGCA TGAGTGGGTG
    CAATGTCTAG AACGATTTCG AGCTGAAGAA AAAGGTTGGG GAATCAGAAC CAAAGAACCC
    CTAAAAGCTG GGCAGTTCAT CATTGAATAC CTAGGAGAGG TTGTCAGCGA GCAGGAGTTC
    AGGAACAGGA TGATTGAGCA GTATCATAAT CATAGTGACC ACTATTGTCT GAACTTGGAT
    AGTGGGATGG TGATTGACAG TTACCGTATG GGAAATGAGG CCCGATTCAT CAATCACAGC
    TGTGACCCAA ATTGTGAAAT GCAGAAATGG TCTGTTAATG GAGTGTACCG GATTGGACTA
    TATGCTCTTA AAGATATGCC AGCTGGTACT GAACTCACTT ATGATTACAA CTTTCATTCC
    TTCAACGTTG AAAAACAGCA ACTGTGTAAG TGCGGCTTTG AGAAATGTCG AGGAATCATT
    GGGGGCAAGA GTCAACGCAT GAATGGACTC ACCAGCAACA AAAGTAGCCA ACCAGTGACC
    TCACATAAAA AGTCTGGACG GTCAAAAGAG AAAAGGAAGT CTAAGCACAA GCTGAAGAAA
    AGGAGAGGCC ACCTCTCTGA GGAACCCAGT GAAAACATCA ACACTCCAAC AAGGTTGACC
    CCTCAATTAC AAATGAAGCC AATGTCCAAT CGGGAGAGAA ACTTTGTATT AAAGCATCAT
    GTGTTCCTGG TCCGAAACTG GGAGAAGATT CGGCAGAAAC AGGAGGAAGT AAAGCACACC
    AGTGATAATA TTCATTCAGC ATCATTATAT ACCCGTTGGA ATGGCATCTG CCGAGATGAT
    GGGAACATCA AGTCTGATGT CTTCATGACC CAGTTCTCTG CCCTGCAGAC TGCTCGATCT
    GTTCGAACAA GACGGTTAGC AGCAGCAGAG GAAAACATTG AAGTAGCTCG GGCAGCTCGC
    CTAGCCCAGA TCTTCAAAGA GATCTGTGAT GGCATAATCT CTTATAAAGA TTCTTCCCGA
    CAATCACTAG CAGCACCACT TTTGAACCTA CCCCCAAAGA AAAAGAATGC TGATTATTAT
    GAGAAGATCT CTGATCCCCT AGATCTTTCC ACCATAGAGA AGCAGATCCT CATTGGTTAT
    TATAAGACAG TGGAGGCTTT TGATGCTGAC ATGCTCAAGG TCTTTCGGAA TGCAGAGAAG
    TACTACGGGC GTAAATCCCC AGTTGGAAGA GATGTTTGCC GCCTACGAAA GGCCTATTAC
    AATGCTCGGC ATGAGGCATC AGCCCAAATT GATGAGATTG TGGGAGAGAC AGCAAGTGAG
    GCAGACAGCA GTGAGACCTC AGTCTCAGAG AAGGAGAATG GGCATGAGAA AGACGATGAT
    GTTATCCGCT GTATCTGTGG CCTCTACAAG GATGAAGGCC TCATGATCCA GTGTGACAAG
    TGCATGGTGT GGCAGCACTG TGACTGTATG GGAGTGAACT CAGATGTGGA GCACTACCTT
    TGTGAGCAGT GTGACCCGAG GCCTGTGGAC AGAGAGGTAC CCATGATCCC TCGGCCCCAC
    TACACGCAAC CTGGTTGTGT CTACTTCATC TGTTTGCTCC GAGATGACCT GCTGCTTCGT
    CAAGGTGACT GTGTATATCT GATGAGGGAT AGTCGGCGCA CCCCTGATGG CCACCCTGTC
    CGTCAGTCCT ATCGACTGTT ATCTCACATT AATCGAGATA AACTTGACAT ATTCCGCATT
    GAGAAGCTTT GGAAAAATGA AAAAGAGGAA CGGTTTGCCT TTGGTCACCA TTATTTTCGT
    CCCCATGAAA CCCACCACTC TCCATCCCGT CGGTTTTATC ACAATGAACT CTTTCGAGTG
    CCACTTTATG AGATCATTCC CTTGGAGGCT GTAGTGGGGA CCTGCTGTGT ACTGGATCTT
    TATACATACT GTAAAGGGAG ACCCAAAGGA GTGAAGGAGC AAGATGTGTA CATCTGTGAT
    TATCGGCTAG ACAAGTCAGC ACACCTGTTT TACAAGATCC ACCGGAACCG CTATCCTGTC
    TGCACCAAAC CCTATGCCTT TGATCACTTC CCCAAGAAGC TCACTCCAAA AAGAGATTTC
    TCAATCATCC TGGAAGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.