MBD1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MBD1
Entrez Gene ID	540558
Full Name	methyl-CpG binding domain protein 1
General protein information	Preferred Names methyl-CpG binding domain protein 1 Names methyl-CpG-binding domain protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010818929.1	XP_010817231.1	methyl-CpG-binding domain protein 1 isoform X4
XM_010818930.1	XP_010817232.1	methyl-CpG-binding domain protein 1 isoform X6
XM_005224252.3	XP_005224309.1	methyl-CpG-binding domain protein 1 isoform X3
XM_005224253.3	XP_005224310.1	methyl-CpG-binding domain protein 1 isoform X4
XM_024984376.1	XP_024840144.1	methyl-CpG-binding domain protein 1 isoform X6
XM_024984378.1	XP_024840146.1	methyl-CpG-binding domain protein 1 isoform X8
XM_010818930.2	XP_010817232.1	methyl-CpG-binding domain protein 1 isoform X11
XM_005224252.2	XP_005224309.1	methyl-CpG-binding domain protein 1 isoform X1
XM_005224257.2	XP_005224314.1	methyl-CpG-binding domain protein 1 isoform X3
XM_010818931.1	XP_010817233.1	methyl-CpG-binding domain protein 1 isoform X7
XM_024984374.1	XP_024840142.1	methyl-CpG-binding domain protein 1 isoform X1
XM_024984375.1	XP_024840143.1	methyl-CpG-binding domain protein 1 isoform X2
XM_005224260.3	XP_005224317.1	methyl-CpG-binding domain protein 1 isoform X10
NM_001046481.2	NP_001039946.1	methyl-CpG-binding domain protein 1
XM_005224253.2	XP_005224310.1	methyl-CpG-binding domain protein 1 isoform X2
XM_005224257.3	XP_005224314.1	methyl-CpG-binding domain protein 1 isoform X5
XM_024984377.1	XP_024840145.1	methyl-CpG-binding domain protein 1 isoform X7
XM_005224260.2	XP_005224317.1	methyl-CpG-binding domain protein 1 isoform X5
XM_010818929.2	XP_010817231.1	methyl-CpG-binding domain protein 1 isoform X9
XM_010818931.2	XP_010817233.1	methyl-CpG-binding domain protein 1 isoform X12

Pathways from BioSystems

Homologies

Homo sapiens (human)	MBD1	NP_001191065.1
Bos taurus (cattle)	MBD1	NP_001039946.1
Mus musculus (house mouse)	Mbd1	NP_038622.2
Canis lupus familiaris (dog)	MBD1	XP_005623163.1
Rattus norvegicus (Norway rat)	Mbd1	NP_001011924.1
Pan troglodytes (chimpanzee)	MBD1	XP_003315950.1

Gene Ontology
Bibliography

Related articles in PubMed

Unexpected cross-species contamination in genome sequencing projects.
Merchant S, Wood DE, Salzberg SL
PeerJ2e675(2014)

The genome sequence of taurine cattle: a window to ruminant biology and evolution.
, Elsik CG, Tellam RL, Worley KC, Gibbs RA, Muzny DM, Weinstock GM, Adelson DL, Eichler EE, Elnitski L, Guig? R, Hamernik DL, Kappes SM, Lewin HA, Lynn DJ, Nicholas FW, Reymond A, Rijnkels M, Skow LC, Zdobnov EM, Schook L, Womack J, Alioto T, Antonarakis SE, Astashyn A, Chapple CE, Chen HC, Chrast J, C?mara F, Ermolaeva O, Henrichsen CN, Hlavina W, Kapustin Y, Kiryutin B, Kitts P, Kokocinski F, Landrum M, Maglott D, Pruitt K, Sapojnikov V, Searle SM, Solovyev V, Souvorov A, Ucla C, Wyss C, Anzola JM, Gerlach D, Elhaik E, Graur D, Reese JT, Edgar RC, McEwan JC, Payne GM, Raison JM, Junier T, Kriventseva EV, Eyras E, Plass M, Donthu R, Larkin DM, Reecy J, Yang MQ, Chen L, Cheng Z, Chitko-McKown CG, Liu GE, Matukumalli LK, Song J, Zhu B, Bradley DG, Brinkman FS, Lau LP, Whiteside MD, Walker A, Wheeler TT, Casey T, German JB, Lemay DG, Maqbool NJ, Molenaar AJ, Seo S, Stothard P, Baldwin CL, Baxter R, Brinkmeyer-Langford CL, Brown WC, Childers CP, Connelley T, Ellis SA, Fritz K, Glass EJ, Herzig CT, Iivanainen A, Lahmers KK, Bennett AK, Dickens CM, Gilbert JG, Hagen DE, Salih H, Aerts J, Caetano AR, Dalrymple B, Garcia JF, Gill CA, Hiendleder SG, Memili E, Spurlock D, Williams JL, Alexander L, Brownstein MJ, Guan L, Holt RA, Jones SJ, Marra MA, Moore R, Moore SS, Roberts A, Taniguchi M, Waterman RC, Chacko J, Chandrabose MM, Cree A, Dao MD, Dinh HH, Gabisi RA, Hines S, Hume J, Jhangiani SN, Joshi V, Kovar CL, Lewis LR, Liu YS, Lopez J, Morgan MB, Nguyen NB, Okwuonu GO, Ruiz SJ, Santibanez J, Wright RA, Buhay C, Ding Y, Dugan-Rocha S, Herdandez J, Holder M, Sabo A, Egan A, Goodell J, Wilczek-Boney K, Fowler GR, Hitchens ME, Lozado RJ, Moen C, Steffen D, Warren JT, Zhang J, Chiu R, Schein JE, Durbin KJ, Havlak P, Jiang H, Liu Y, Qin X, Ren Y, Shen Y, Song H, Bell SN, Davis C, Johnson AJ, Lee S, Nazareth LV, Patel BM, Pu LL, Vattathil S, Williams RL Jr, Curry S, Hamilton C, Sodergren E, Wheeler DA, Barris W, Bennett GL, Eggen A, Green RD, Harhay GP, Hobbs M, Jann O, Keele JW, Kent MP, Lien S, McKay SD, McWilliam S, Ratnakumar A, Schnabel RD, Smith T, Snelling WM, Sonstegard TS, Stone RT, Sugimoto Y, Takasuga A, Taylor JF, Van Tassell CP, Macneil MD, Abatepaulo AR, Abbey CA, Ahola V, Almeida IG, Amadio AF, Anatriello E, Bahadue SM, Biase FH, Boldt CR, Carroll JA, Carvalho WA, Cervelatti EP, Chacko E, Chapin JE, Cheng Y, Choi J, Colley AJ, de Campos TA, De Donato M, Santos IK, de Oliveira CJ, Deobald H, Devinoy E, Donohue KE, Dovc P, Eberlein A, Fitzsimmons CJ, Franzin AM, Garcia GR, Genini S, Gladney CJ, Grant JR, Greaser ML, Green JA, Hadsell DL, Hakimov HA, Halgren R, Harrow JL, Hart EA, Hastings N, Hernandez M, Hu ZL, Ingham A, Iso-Touru T, Jamis C, Jensen K, Kapetis D, Kerr T, Khalil SS, Khatib H, Kolbehdari D, Kumar CG, Kumar D, Leach R, Lee JC, Li C, Logan KM, Malinverni R, Marques E, Martin WF, Martins NF, Maruyama SR, Mazza R, McLean KL, Medrano JF, Moreno BT, Mor? DD, Muntean CT, Nandakumar HP, Nogueira MF, Olsaker I, Pant SD, Panzitta F, Pastor RC, Poli MA, Poslusny N, Rachagani S, Ranganathan S, Razpet A, Riggs PK, Rincon G, Rodriguez-Osorio N, Rodriguez-Zas SL, Romero NE, Rosenwald A, Sando L, Schmutz SM, Shen L, Sherman L, Southey BR, Lutzow YS, Sweedler JV, Tammen I, Telugu BP, Urbanski JM, Utsunomiya YT, Verschoor CP, Waardenberg AJ, Wang Z, Ward R, Weikard R, Welsh TH Jr, White SN, Wilming LG, Wunderlich KR, Yang J, Zhao FQ
Science (New York, N.Y.)324(5926)522-8(2009 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MBD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MBD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40772	NM_001046481.2 Latest version!	Bos taurus methyl-CpG binding domain protein 1 (MBD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32609	XM_005224252.3 Latest version!	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32610	XM_005224253.3 Latest version!	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32612	XM_005224257.3 Latest version!	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32615	XM_005224260.3 Latest version!	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa54710	XM_010818929.2 Latest version!	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa54711	XM_010818930.2 Latest version!	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa54712	XM_010818931.2 Latest version!	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa229973	XM_024984374.1 Latest version!	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa229974	XM_024984375.1 Latest version!	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa229975	XM_024984376.1 Latest version!	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa229976	XM_024984377.1 Latest version!	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa229977	XM_024984378.1 Latest version!	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OBa54711	XM_010818930.1	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32609	XM_005224252.2	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32610	XM_005224253.2	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32612	XM_005224257.2	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32615	XM_005224260.2	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa54710	XM_010818929.1	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa54712	XM_010818931.1	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MBD1 ( NM_001046481.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001039946.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), mRNA.

Price & Availability↑

CloneID	OBa40772
Clone ID Related Accession (Same CDS sequence)	NM_001046481.2
Accession Version	NM_001046481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC112458.1. On Aug 31, 2012 this sequence version replaced NM_001046481.1. ##Evidence-Data-START## Transcript exon combination :: BC112458.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGCACCTTGC TCACCGCCTC CGTCGCCACC ATCAGCGATG TCAACGACGC 
CCTCCCCTGG CTGTGGCTCC CCCTGCTGGT AAACACGGCC GCCGCAGAGG AGGCTGCGAC 
TCCAAGGTGG TGCCCCGGCG GCGTCCACCC CGAGCCCAGT CACCGCCTCC ACCTCCCCCA 
CCGCAGCCTC CAGAGTCTCC GGAGCTGCAC CCCAGAGCCC TGGCCCCCTC GCCACCTGCT 
GAATTCATCT ATTACTGTGT AGACGAGGAC GAGCTACAGC CTTACACAAA CCGTCGGCAG 
AACCGCAAGT GTGGGACCTG TGCAGCCTGC CTGCGGCGGA TGGACTGTGG CCACTGCGAC 
TTCTGCTGTG ACAAGCCCAA GTTTGGGGGC AGCAACCAGA AGCGCCAGAA GTGTCGCTGG 
CGCCAGTGCC TGCAGTTTGC TATGAAGCGG CTGCTGCCAA GTGTCTGGGC AGGGTCCGAA 
GATGGGGCAT CGCCGCCCCC AGCTCACCCT CGTCGAAAGA GGCCTGGCTC TACTCGAAGG 
CCCCATCTGG GTCAGACCCT GAAGCCTCCC TTGGCCACAC CCGCAGCTCA ACCAGACCGA 
GCCCAGACTC CAGTGAAGGA GGAAGCAGGC AGTGGCTTTG TGCTGCCCCC ACCTGGCACT 
GACCTTGTGT TCTTACGGGA GGGTGCAAGC AGTCCCGTGC AGGTGCCTGG CCCAGCCCCG 
GCTTCCACAG AAACTCGGTT GCAGGAGGCC CAGTGCCCTG GCCTGAGTTG GGTTGTGGCC 
TTACCCCAGG TGAAGCAAGA GAAGGCGGAT GCCCAGGAAG ACTGGACACC TGGCACAGCC 
ATCCTGACTT CTCCTGTATT GCTGCCTGGC TGCCCCAGCA AGGCAGTAGA CCCAGGCCTG 
CCAGCAGTGA AGCAAGAGCC ACCTGACCCT GAGGAGGACA AGGACGACAA CAAGGCTGAC 
TCCACCTCTG ACTTGCCCCC AGAGGAGGAG GCAGGAGGGG CTGGCACGCC CGTGATCACG 
GAGATTTTCA GCCTGGGTGG AACCCGCCTC CGGGACACAG CAGTCTGGTT GCCAAGGCTA 
CGTAAACTCT TAGCAGTAAA TGAAAATGAG TATTTTACCG AACTGCAATT GAAAGAAGAA 
GCATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039946.1
CDS	195..2123
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRHLAHRLRRHHQRCQRRPPLAVAPPAGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQEAQCPGLSWVVALPQVKQEKADAQEDWTPGTAILTSPVLLPGCPSKAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPRLRKLLAVNENEYFTELQLKEEAL

Target ORF information:

RefSeq Version	NM_001046481.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001046481.2

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGCACCTTGC TCACCGCCTC CGTCGCCACC ATCAGCGATG TCAACGACGC 
CCTCCCCTGG CTGTGGCTCC CCCTGCTGGT AAACACGGCC GCCGCAGAGG AGGCTGCGAC 
TCCAAGGTGG TGCCCCGGCG GCGTCCACCC CGAGCCCAGT CACCGCCTCC ACCTCCCCCA 
CCGCAGCCTC CAGAGTCTCC GGAGCTGCAC CCCAGAGCCC TGGCCCCCTC GCCACCTGCT 
GAATTCATCT ATTACTGTGT AGACGAGGAC GAGCTACAGC CTTACACAAA CCGTCGGCAG 
AACCGCAAGT GTGGGACCTG TGCAGCCTGC CTGCGGCGGA TGGACTGTGG CCACTGCGAC 
TTCTGCTGTG ACAAGCCCAA GTTTGGGGGC AGCAACCAGA AGCGCCAGAA GTGTCGCTGG 
CGCCAGTGCC TGCAGTTTGC TATGAAGCGG CTGCTGCCAA GTGTCTGGGC AGGGTCCGAA 
GATGGGGCAT CGCCGCCCCC AGCTCACCCT CGTCGAAAGA GGCCTGGCTC TACTCGAAGG 
CCCCATCTGG GTCAGACCCT GAAGCCTCCC TTGGCCACAC CCGCAGCTCA ACCAGACCGA 
GCCCAGACTC CAGTGAAGGA GGAAGCAGGC AGTGGCTTTG TGCTGCCCCC ACCTGGCACT 
GACCTTGTGT TCTTACGGGA GGGTGCAAGC AGTCCCGTGC AGGTGCCTGG CCCAGCCCCG 
GCTTCCACAG AAACTCGGTT GCAGGAGGCC CAGTGCCCTG GCCTGAGTTG GGTTGTGGCC 
TTACCCCAGG TGAAGCAAGA GAAGGCGGAT GCCCAGGAAG ACTGGACACC TGGCACAGCC 
ATCCTGACTT CTCCTGTATT GCTGCCTGGC TGCCCCAGCA AGGCAGTAGA CCCAGGCCTG 
CCAGCAGTGA AGCAAGAGCC ACCTGACCCT GAGGAGGACA AGGACGACAA CAAGGCTGAC 
TCCACCTCTG ACTTGCCCCC AGAGGAGGAG GCAGGAGGGG CTGGCACGCC CGTGATCACG 
GAGATTTTCA GCCTGGGTGG AACCCGCCTC CGGGACACAG CAGTCTGGTT GCCAAGGCTA 
CGTAAACTCT TAGCAGTAAA TGAAAATGAG TATTTTACCG AACTGCAATT GAAAGAAGAA 
GCATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_005224252.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224309.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa32609
Clone ID Related Accession (Same CDS sequence)	XM_005224252.3 , XM_005224252.2
Accession Version	XM_005224252.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224252.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG AGGCCCAGTG CCCTGGCCTG AGTTGGGTTG TGGCCTTACC CCAGGTGAAG 
CAAGAGAAGG CGGATGCCCA GGAAGACTGG ACACCTGGCA CAGCCATCCT GACTTCTCCT 
GTATTGCTGC CTGGCTGCCC CAGCAAGGCA GTAGACCCAG GCCTGCCAGC AGTGAAGCAA 
GAGCCACCTG ACCCTGAGGA GGACAAGGAC GACAACAAGG CTGACTCCAC CTCTGACTTG 
CCCCCAGAGG AGGAGGCAGG AGGGGCTGGC ACGCCCGTGA TCACGGAGAT TTTCAGCCTG 
GGTGGAACCC GCCTCCGGGA CACAGCAGTC TGGTTGCCAA GTCTGCAGGG CAGGCAATCG 
GGAAGGGAAG ATGGATGTAA AAAGTGGGAG ACGGAGGACA CACTGGCGCC CATGAGCACG 
AGCTGGAAAC CACAAGGATG GCCTGGAAGC CATGTCAGCC TCTCACCACC TCCAACTTCG 
GTGACGTGGG TGTCCTGCAG AAGAAGCTGG TGCCCTTCAT CACAGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224309.1
CDS	190..2160
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQEAQCPGLSWVVALPQVKQEKADAQEDWTPGTAILTSPVLLPGCPSKAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPSLQGRQSGREDGCKKWETEDTLAPMSTSWKPQGWPGSHVSLSPPPTSVTWVSCRRSWCPSSQS

Target ORF information:

RefSeq Version	XM_005224252.3
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224252.3

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG AGGCCCAGTG CCCTGGCCTG AGTTGGGTTG TGGCCTTACC CCAGGTGAAG 
CAAGAGAAGG CGGATGCCCA GGAAGACTGG ACACCTGGCA CAGCCATCCT GACTTCTCCT 
GTATTGCTGC CTGGCTGCCC CAGCAAGGCA GTAGACCCAG GCCTGCCAGC AGTGAAGCAA 
GAGCCACCTG ACCCTGAGGA GGACAAGGAC GACAACAAGG CTGACTCCAC CTCTGACTTG 
CCCCCAGAGG AGGAGGCAGG AGGGGCTGGC ACGCCCGTGA TCACGGAGAT TTTCAGCCTG 
GGTGGAACCC GCCTCCGGGA CACAGCAGTC TGGTTGCCAA GTCTGCAGGG CAGGCAATCG 
GGAAGGGAAG ATGGATGTAA AAAGTGGGAG ACGGAGGACA CACTGGCGCC CATGAGCACG 
AGCTGGAAAC CACAAGGATG GCCTGGAAGC CATGTCAGCC TCTCACCACC TCCAACTTCG 
GTGACGTGGG TGTCCTGCAG AAGAAGCTGG TGCCCTTCAT CACAGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_005224252.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224309.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32609
Clone ID Related Accession (Same CDS sequence)	XM_005224252.3 , XM_005224252.2
Accession Version	XM_005224252.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005224252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG AGGCCCAGTG CCCTGGCCTG AGTTGGGTTG TGGCCTTACC CCAGGTGAAG 
CAAGAGAAGG CGGATGCCCA GGAAGACTGG ACACCTGGCA CAGCCATCCT GACTTCTCCT 
GTATTGCTGC CTGGCTGCCC CAGCAAGGCA GTAGACCCAG GCCTGCCAGC AGTGAAGCAA 
GAGCCACCTG ACCCTGAGGA GGACAAGGAC GACAACAAGG CTGACTCCAC CTCTGACTTG 
CCCCCAGAGG AGGAGGCAGG AGGGGCTGGC ACGCCCGTGA TCACGGAGAT TTTCAGCCTG 
GGTGGAACCC GCCTCCGGGA CACAGCAGTC TGGTTGCCAA GTCTGCAGGG CAGGCAATCG 
GGAAGGGAAG ATGGATGTAA AAAGTGGGAG ACGGAGGACA CACTGGCGCC CATGAGCACG 
AGCTGGAAAC CACAAGGATG GCCTGGAAGC CATGTCAGCC TCTCACCACC TCCAACTTCG 
GTGACGTGGG TGTCCTGCAG AAGAAGCTGG TGCCCTTCAT CACAGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224309.1
CDS	190..2160
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQEAQCPGLSWVVALPQVKQEKADAQEDWTPGTAILTSPVLLPGCPSKAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPSLQGRQSGREDGCKKWETEDTLAPMSTSWKPQGWPGSHVSLSPPPTSVTWVSCRRSWCPSSQS

Target ORF information:

RefSeq Version	XM_005224252.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224252.2

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG AGGCCCAGTG CCCTGGCCTG AGTTGGGTTG TGGCCTTACC CCAGGTGAAG 
CAAGAGAAGG CGGATGCCCA GGAAGACTGG ACACCTGGCA CAGCCATCCT GACTTCTCCT 
GTATTGCTGC CTGGCTGCCC CAGCAAGGCA GTAGACCCAG GCCTGCCAGC AGTGAAGCAA 
GAGCCACCTG ACCCTGAGGA GGACAAGGAC GACAACAAGG CTGACTCCAC CTCTGACTTG 
CCCCCAGAGG AGGAGGCAGG AGGGGCTGGC ACGCCCGTGA TCACGGAGAT TTTCAGCCTG 
GGTGGAACCC GCCTCCGGGA CACAGCAGTC TGGTTGCCAA GTCTGCAGGG CAGGCAATCG 
GGAAGGGAAG ATGGATGTAA AAAGTGGGAG ACGGAGGACA CACTGGCGCC CATGAGCACG 
AGCTGGAAAC CACAAGGATG GCCTGGAAGC CATGTCAGCC TCTCACCACC TCCAACTTCG 
GTGACGTGGG TGTCCTGCAG AAGAAGCTGG TGCCCTTCAT CACAGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_005224253.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224310.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa32610
Clone ID Related Accession (Same CDS sequence)	XM_005224253.3 , XM_005224253.2
Accession Version	XM_005224253.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224253.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGCACCTTGC TCACCGCCTC CGTCGCCACC ATCAGCGATG TCAACGACGC 
CCTCCCCTGG CTGTGGCTCC CCCTGCTGGT AAACACGGCC GCCGCAGAGG AGGCTGCGAC 
TCCAAGGTGG TGCCCCGGCG GCGTCCACCC CGAGCCCAGT CACCGCCTCC ACCTCCCCCA 
CCGCAGCCTC CAGAGTCTCC GGAGCTGCAC CCCAGAGCCC TGGCCCCCTC GCCACCTGCT 
GAATTCATCT ATTACTGTGT AGACGAGGAC GAGCTACAGC CTTACACAAA CCGTCGGCAG 
AACCGCAAGT GTGGGACCTG TGCAGCCTGC CTGCGGCGGA TGGACTGTGG CCACTGCGAC 
TTCTGCTGTG ACAAGCCCAA GTTTGGGGGC AGCAACCAGA AGCGCCAGAA GTGTCGCTGG 
CGCCAGTGCC TGCAGTTTGC TATGAAGCGG CTGCTGCCAA GTGTCTGGGC AGGGTCCGAA 
GATGGGGCAT CGCCGCCCCC AGCTCACCCT CGTCGAAAGA GGCCTGGCTC TACTCGAAGG 
CCCCATCTGG GTCAGACCCT GAAGCCTCCC TTGGCCACAC CCGCAGCTCA ACCAGACCGA 
GCCCAGACTC CAGTGAAGGA GGAAGCAGGC AGTGGCTTTG TGCTGCCCCC ACCTGGCACT 
GACCTTGTGT TCTTACGGGA GGGTGCAAGC AGTCCCGTGC AGGTGCCTGG CCCAGCCCCG 
GCTTCCACAG AAACTCGGTT GCAGGAGGCC CAGTGCCCTG GCCTGAGTTG GGTTGTGGCC 
TTACCCCAGG TGAAGCAAGA GAAGGCGGAT GCCCAGGAAG ACTGGACACC TGGCACAGCC 
ATCCTGACTT CTCCTGTATT GCTGCCTGGC TGCCCCAGCA AGGCAGTAGA CCCAGGCCTG 
CCAGCAGTGA AGCAAGAGCC ACCTGACCCT GAGGAGGACA AGGACGACAA CAAGGCTGAC 
TCCACCTCTG ACTTGCCCCC AGAGGAGGAG GCAGGAGGGG CTGGCACGCC CGTGATCACG 
GAGATTTTCA GCCTGGGTGG AACCCGCCTC CGGGACACAG CAGTCTGGTT GCCAAGGTCC 
AAGGACCTTA AAAAACCTGG AGGTAGAAAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224310.1
CDS	190..2085
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRHLAHRLRRHHQRCQRRPPLAVAPPAGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQEAQCPGLSWVVALPQVKQEKADAQEDWTPGTAILTSPVLLPGCPSKAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPRSKDLKKPGGRKQ

Target ORF information:

RefSeq Version	XM_005224253.3
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224253.3

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGCACCTTGC TCACCGCCTC CGTCGCCACC ATCAGCGATG TCAACGACGC 
CCTCCCCTGG CTGTGGCTCC CCCTGCTGGT AAACACGGCC GCCGCAGAGG AGGCTGCGAC 
TCCAAGGTGG TGCCCCGGCG GCGTCCACCC CGAGCCCAGT CACCGCCTCC ACCTCCCCCA 
CCGCAGCCTC CAGAGTCTCC GGAGCTGCAC CCCAGAGCCC TGGCCCCCTC GCCACCTGCT 
GAATTCATCT ATTACTGTGT AGACGAGGAC GAGCTACAGC CTTACACAAA CCGTCGGCAG 
AACCGCAAGT GTGGGACCTG TGCAGCCTGC CTGCGGCGGA TGGACTGTGG CCACTGCGAC 
TTCTGCTGTG ACAAGCCCAA GTTTGGGGGC AGCAACCAGA AGCGCCAGAA GTGTCGCTGG 
CGCCAGTGCC TGCAGTTTGC TATGAAGCGG CTGCTGCCAA GTGTCTGGGC AGGGTCCGAA 
GATGGGGCAT CGCCGCCCCC AGCTCACCCT CGTCGAAAGA GGCCTGGCTC TACTCGAAGG 
CCCCATCTGG GTCAGACCCT GAAGCCTCCC TTGGCCACAC CCGCAGCTCA ACCAGACCGA 
GCCCAGACTC CAGTGAAGGA GGAAGCAGGC AGTGGCTTTG TGCTGCCCCC ACCTGGCACT 
GACCTTGTGT TCTTACGGGA GGGTGCAAGC AGTCCCGTGC AGGTGCCTGG CCCAGCCCCG 
GCTTCCACAG AAACTCGGTT GCAGGAGGCC CAGTGCCCTG GCCTGAGTTG GGTTGTGGCC 
TTACCCCAGG TGAAGCAAGA GAAGGCGGAT GCCCAGGAAG ACTGGACACC TGGCACAGCC 
ATCCTGACTT CTCCTGTATT GCTGCCTGGC TGCCCCAGCA AGGCAGTAGA CCCAGGCCTG 
CCAGCAGTGA AGCAAGAGCC ACCTGACCCT GAGGAGGACA AGGACGACAA CAAGGCTGAC 
TCCACCTCTG ACTTGCCCCC AGAGGAGGAG GCAGGAGGGG CTGGCACGCC CGTGATCACG 
GAGATTTTCA GCCTGGGTGG AACCCGCCTC CGGGACACAG CAGTCTGGTT GCCAAGGTCC 
AAGGACCTTA AAAAACCTGG AGGTAGAAAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_005224253.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224310.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa32610
Clone ID Related Accession (Same CDS sequence)	XM_005224253.3 , XM_005224253.2
Accession Version	XM_005224253.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005224253.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGCACCTTGC TCACCGCCTC CGTCGCCACC ATCAGCGATG TCAACGACGC 
CCTCCCCTGG CTGTGGCTCC CCCTGCTGGT AAACACGGCC GCCGCAGAGG AGGCTGCGAC 
TCCAAGGTGG TGCCCCGGCG GCGTCCACCC CGAGCCCAGT CACCGCCTCC ACCTCCCCCA 
CCGCAGCCTC CAGAGTCTCC GGAGCTGCAC CCCAGAGCCC TGGCCCCCTC GCCACCTGCT 
GAATTCATCT ATTACTGTGT AGACGAGGAC GAGCTACAGC CTTACACAAA CCGTCGGCAG 
AACCGCAAGT GTGGGACCTG TGCAGCCTGC CTGCGGCGGA TGGACTGTGG CCACTGCGAC 
TTCTGCTGTG ACAAGCCCAA GTTTGGGGGC AGCAACCAGA AGCGCCAGAA GTGTCGCTGG 
CGCCAGTGCC TGCAGTTTGC TATGAAGCGG CTGCTGCCAA GTGTCTGGGC AGGGTCCGAA 
GATGGGGCAT CGCCGCCCCC AGCTCACCCT CGTCGAAAGA GGCCTGGCTC TACTCGAAGG 
CCCCATCTGG GTCAGACCCT GAAGCCTCCC TTGGCCACAC CCGCAGCTCA ACCAGACCGA 
GCCCAGACTC CAGTGAAGGA GGAAGCAGGC AGTGGCTTTG TGCTGCCCCC ACCTGGCACT 
GACCTTGTGT TCTTACGGGA GGGTGCAAGC AGTCCCGTGC AGGTGCCTGG CCCAGCCCCG 
GCTTCCACAG AAACTCGGTT GCAGGAGGCC CAGTGCCCTG GCCTGAGTTG GGTTGTGGCC 
TTACCCCAGG TGAAGCAAGA GAAGGCGGAT GCCCAGGAAG ACTGGACACC TGGCACAGCC 
ATCCTGACTT CTCCTGTATT GCTGCCTGGC TGCCCCAGCA AGGCAGTAGA CCCAGGCCTG 
CCAGCAGTGA AGCAAGAGCC ACCTGACCCT GAGGAGGACA AGGACGACAA CAAGGCTGAC 
TCCACCTCTG ACTTGCCCCC AGAGGAGGAG GCAGGAGGGG CTGGCACGCC CGTGATCACG 
GAGATTTTCA GCCTGGGTGG AACCCGCCTC CGGGACACAG CAGTCTGGTT GCCAAGGTCC 
AAGGACCTTA AAAAACCTGG AGGTAGAAAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224310.1
CDS	190..2085
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRHLAHRLRRHHQRCQRRPPLAVAPPAGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQEAQCPGLSWVVALPQVKQEKADAQEDWTPGTAILTSPVLLPGCPSKAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPRSKDLKKPGGRKQ

Target ORF information:

RefSeq Version	XM_005224253.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224253.2

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGCACCTTGC TCACCGCCTC CGTCGCCACC ATCAGCGATG TCAACGACGC 
CCTCCCCTGG CTGTGGCTCC CCCTGCTGGT AAACACGGCC GCCGCAGAGG AGGCTGCGAC 
TCCAAGGTGG TGCCCCGGCG GCGTCCACCC CGAGCCCAGT CACCGCCTCC ACCTCCCCCA 
CCGCAGCCTC CAGAGTCTCC GGAGCTGCAC CCCAGAGCCC TGGCCCCCTC GCCACCTGCT 
GAATTCATCT ATTACTGTGT AGACGAGGAC GAGCTACAGC CTTACACAAA CCGTCGGCAG 
AACCGCAAGT GTGGGACCTG TGCAGCCTGC CTGCGGCGGA TGGACTGTGG CCACTGCGAC 
TTCTGCTGTG ACAAGCCCAA GTTTGGGGGC AGCAACCAGA AGCGCCAGAA GTGTCGCTGG 
CGCCAGTGCC TGCAGTTTGC TATGAAGCGG CTGCTGCCAA GTGTCTGGGC AGGGTCCGAA 
GATGGGGCAT CGCCGCCCCC AGCTCACCCT CGTCGAAAGA GGCCTGGCTC TACTCGAAGG 
CCCCATCTGG GTCAGACCCT GAAGCCTCCC TTGGCCACAC CCGCAGCTCA ACCAGACCGA 
GCCCAGACTC CAGTGAAGGA GGAAGCAGGC AGTGGCTTTG TGCTGCCCCC ACCTGGCACT 
GACCTTGTGT TCTTACGGGA GGGTGCAAGC AGTCCCGTGC AGGTGCCTGG CCCAGCCCCG 
GCTTCCACAG AAACTCGGTT GCAGGAGGCC CAGTGCCCTG GCCTGAGTTG GGTTGTGGCC 
TTACCCCAGG TGAAGCAAGA GAAGGCGGAT GCCCAGGAAG ACTGGACACC TGGCACAGCC 
ATCCTGACTT CTCCTGTATT GCTGCCTGGC TGCCCCAGCA AGGCAGTAGA CCCAGGCCTG 
CCAGCAGTGA AGCAAGAGCC ACCTGACCCT GAGGAGGACA AGGACGACAA CAAGGCTGAC 
TCCACCTCTG ACTTGCCCCC AGAGGAGGAG GCAGGAGGGG CTGGCACGCC CGTGATCACG 
GAGATTTTCA GCCTGGGTGG AACCCGCCTC CGGGACACAG CAGTCTGGTT GCCAAGGTCC 
AAGGACCTTA AAAAACCTGG AGGTAGAAAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_005224257.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224314.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa32612
Clone ID Related Accession (Same CDS sequence)	XM_005224257.2 , XM_005224257.3
Accession Version	XM_005224257.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005224257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG AGGCCCAGTG CCCTGGCCTG AGTTGGGTTG TGGCCTTACC CCAGGTGAAG 
CAAGAGAAGG CGGATGCCCA GGAAGACTGG ACACCTGGCA CAGCCATCCT GACTTCTCCT 
GTATTGCTGC CTGGCTGCCC CAGCAAGGCA GTAGACCCAG GCCTGCCAGC AGTGAAGCAA 
GAGCCACCTG ACCCTGAGGA GGACAAGGAC GACAACAAGG CTGACTCCAC CTCTGACTTG 
CCCCCAGAGG AGGAGGCAGG AGGGGCTGGC ACGCCCGTGA TCACGGAGAT TTTCAGCCTG 
GGTGGAACCC GCCTCCGGGA CACAGCAGTC TGGTTGCCAA GGTCCAAGGA CCTTAAAAAA 
CCTGGAGGTA GAAAGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224314.1
CDS	190..2010
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQEAQCPGLSWVVALPQVKQEKADAQEDWTPGTAILTSPVLLPGCPSKAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPRSKDLKKPGGRKQ

Target ORF information:

RefSeq Version	XM_005224257.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224257.2

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG AGGCCCAGTG CCCTGGCCTG AGTTGGGTTG TGGCCTTACC CCAGGTGAAG 
CAAGAGAAGG CGGATGCCCA GGAAGACTGG ACACCTGGCA CAGCCATCCT GACTTCTCCT 
GTATTGCTGC CTGGCTGCCC CAGCAAGGCA GTAGACCCAG GCCTGCCAGC AGTGAAGCAA 
GAGCCACCTG ACCCTGAGGA GGACAAGGAC GACAACAAGG CTGACTCCAC CTCTGACTTG 
CCCCCAGAGG AGGAGGCAGG AGGGGCTGGC ACGCCCGTGA TCACGGAGAT TTTCAGCCTG 
GGTGGAACCC GCCTCCGGGA CACAGCAGTC TGGTTGCCAA GGTCCAAGGA CCTTAAAAAA 
CCTGGAGGTA GAAAGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_005224257.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224314.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa32612
Clone ID Related Accession (Same CDS sequence)	XM_005224257.2 , XM_005224257.3
Accession Version	XM_005224257.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224257.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG AGGCCCAGTG CCCTGGCCTG AGTTGGGTTG TGGCCTTACC CCAGGTGAAG 
CAAGAGAAGG CGGATGCCCA GGAAGACTGG ACACCTGGCA CAGCCATCCT GACTTCTCCT 
GTATTGCTGC CTGGCTGCCC CAGCAAGGCA GTAGACCCAG GCCTGCCAGC AGTGAAGCAA 
GAGCCACCTG ACCCTGAGGA GGACAAGGAC GACAACAAGG CTGACTCCAC CTCTGACTTG 
CCCCCAGAGG AGGAGGCAGG AGGGGCTGGC ACGCCCGTGA TCACGGAGAT TTTCAGCCTG 
GGTGGAACCC GCCTCCGGGA CACAGCAGTC TGGTTGCCAA GGTCCAAGGA CCTTAAAAAA 
CCTGGAGGTA GAAAGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224314.1
CDS	190..2010
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQEAQCPGLSWVVALPQVKQEKADAQEDWTPGTAILTSPVLLPGCPSKAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPRSKDLKKPGGRKQ

Target ORF information:

RefSeq Version	XM_005224257.3
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224257.3

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG AGGCCCAGTG CCCTGGCCTG AGTTGGGTTG TGGCCTTACC CCAGGTGAAG 
CAAGAGAAGG CGGATGCCCA GGAAGACTGG ACACCTGGCA CAGCCATCCT GACTTCTCCT 
GTATTGCTGC CTGGCTGCCC CAGCAAGGCA GTAGACCCAG GCCTGCCAGC AGTGAAGCAA 
GAGCCACCTG ACCCTGAGGA GGACAAGGAC GACAACAAGG CTGACTCCAC CTCTGACTTG 
CCCCCAGAGG AGGAGGCAGG AGGGGCTGGC ACGCCCGTGA TCACGGAGAT TTTCAGCCTG 
GGTGGAACCC GCCTCCGGGA CACAGCAGTC TGGTTGCCAA GGTCCAAGGA CCTTAAAAAA 
CCTGGAGGTA GAAAGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_005224260.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224317.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa32615
Clone ID Related Accession (Same CDS sequence)	XM_005224260.3 , XM_005224260.2
Accession Version	XM_005224260.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224260.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCAGCCTTA CACAAACCGT CGGCAGAACC GCAAGTGTGG GACCTGTGCA 
GCCTGCCTGC GGCGGATGGA CTGTGGCCAC TGCGACTTCT GCTGTGACAA GCCCAAGTTT 
GGGGGCAGCA ACCAGAAGCG CCAGAAGTGT CGCTGGCGCC AGTGCCTGCA GTTTGCTATG 
AAGCGGCTGC TGCCAAGTGT CTGGGCAGGG TCCGAAGATG GGGCATCGCC GCCCCCAGCT 
CACCCTCGTC GAAAGAGGCC TGGCTCTACT CGAAGGCCCC ATCTGGGTCA GACCCTGAAG 
CCTCCCTTGG CCACACCCGC AGCTCAACCA GACCGAGCCC AGACTCCAGT GAAGGAGGAA 
GCAGGCAGTG GCTTTGTGCT GCCCCCACCT GGCACTGACC TTGTGTTCTT ACGGGAGGGT 
GCAAGCAGTC CCGTGCAGGT GCCTGGCCCA GCCCCGGCTT CCACAGAAAC TCGGTTGCAG 
GCAGTAGACC CAGGCCTGCC AGCAGTGAAG CAAGAGCCAC CTGACCCTGA GGAGGACAAG 
GACGACAACA AGGCTGACTC CACCTCTGAC TTGCCCCCAG AGGAGGAGGC AGGAGGGGCT 
GGCACGCCCG TGATCACGGA GATTTTCAGC CTGGGTGGAA CCCGCCTCCG GGACACAGCA 
GTCTGGTTGC CAAGGTCCAA GGACCTTAAA AAACCTGGAG GTAGAAAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224317.1
CDS	190..1803
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPRSKDLKKPGGRKQ

Target ORF information:

RefSeq Version	XM_005224260.3
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224260.3

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCAGCCTTA CACAAACCGT CGGCAGAACC GCAAGTGTGG GACCTGTGCA 
GCCTGCCTGC GGCGGATGGA CTGTGGCCAC TGCGACTTCT GCTGTGACAA GCCCAAGTTT 
GGGGGCAGCA ACCAGAAGCG CCAGAAGTGT CGCTGGCGCC AGTGCCTGCA GTTTGCTATG 
AAGCGGCTGC TGCCAAGTGT CTGGGCAGGG TCCGAAGATG GGGCATCGCC GCCCCCAGCT 
CACCCTCGTC GAAAGAGGCC TGGCTCTACT CGAAGGCCCC ATCTGGGTCA GACCCTGAAG 
CCTCCCTTGG CCACACCCGC AGCTCAACCA GACCGAGCCC AGACTCCAGT GAAGGAGGAA 
GCAGGCAGTG GCTTTGTGCT GCCCCCACCT GGCACTGACC TTGTGTTCTT ACGGGAGGGT 
GCAAGCAGTC CCGTGCAGGT GCCTGGCCCA GCCCCGGCTT CCACAGAAAC TCGGTTGCAG 
GCAGTAGACC CAGGCCTGCC AGCAGTGAAG CAAGAGCCAC CTGACCCTGA GGAGGACAAG 
GACGACAACA AGGCTGACTC CACCTCTGAC TTGCCCCCAG AGGAGGAGGC AGGAGGGGCT 
GGCACGCCCG TGATCACGGA GATTTTCAGC CTGGGTGGAA CCCGCCTCCG GGACACAGCA 
GTCTGGTTGC CAAGGTCCAA GGACCTTAAA AAACCTGGAG GTAGAAAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_005224260.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224317.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa32615
Clone ID Related Accession (Same CDS sequence)	XM_005224260.3 , XM_005224260.2
Accession Version	XM_005224260.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005224260.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCAGCCTTA CACAAACCGT CGGCAGAACC GCAAGTGTGG GACCTGTGCA 
GCCTGCCTGC GGCGGATGGA CTGTGGCCAC TGCGACTTCT GCTGTGACAA GCCCAAGTTT 
GGGGGCAGCA ACCAGAAGCG CCAGAAGTGT CGCTGGCGCC AGTGCCTGCA GTTTGCTATG 
AAGCGGCTGC TGCCAAGTGT CTGGGCAGGG TCCGAAGATG GGGCATCGCC GCCCCCAGCT 
CACCCTCGTC GAAAGAGGCC TGGCTCTACT CGAAGGCCCC ATCTGGGTCA GACCCTGAAG 
CCTCCCTTGG CCACACCCGC AGCTCAACCA GACCGAGCCC AGACTCCAGT GAAGGAGGAA 
GCAGGCAGTG GCTTTGTGCT GCCCCCACCT GGCACTGACC TTGTGTTCTT ACGGGAGGGT 
GCAAGCAGTC CCGTGCAGGT GCCTGGCCCA GCCCCGGCTT CCACAGAAAC TCGGTTGCAG 
GCAGTAGACC CAGGCCTGCC AGCAGTGAAG CAAGAGCCAC CTGACCCTGA GGAGGACAAG 
GACGACAACA AGGCTGACTC CACCTCTGAC TTGCCCCCAG AGGAGGAGGC AGGAGGGGCT 
GGCACGCCCG TGATCACGGA GATTTTCAGC CTGGGTGGAA CCCGCCTCCG GGACACAGCA 
GTCTGGTTGC CAAGGTCCAA GGACCTTAAA AAACCTGGAG GTAGAAAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224317.1
CDS	190..1803
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPRSKDLKKPGGRKQ

Target ORF information:

RefSeq Version	XM_005224260.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224260.2

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCAGCCTTA CACAAACCGT CGGCAGAACC GCAAGTGTGG GACCTGTGCA 
GCCTGCCTGC GGCGGATGGA CTGTGGCCAC TGCGACTTCT GCTGTGACAA GCCCAAGTTT 
GGGGGCAGCA ACCAGAAGCG CCAGAAGTGT CGCTGGCGCC AGTGCCTGCA GTTTGCTATG 
AAGCGGCTGC TGCCAAGTGT CTGGGCAGGG TCCGAAGATG GGGCATCGCC GCCCCCAGCT 
CACCCTCGTC GAAAGAGGCC TGGCTCTACT CGAAGGCCCC ATCTGGGTCA GACCCTGAAG 
CCTCCCTTGG CCACACCCGC AGCTCAACCA GACCGAGCCC AGACTCCAGT GAAGGAGGAA 
GCAGGCAGTG GCTTTGTGCT GCCCCCACCT GGCACTGACC TTGTGTTCTT ACGGGAGGGT 
GCAAGCAGTC CCGTGCAGGT GCCTGGCCCA GCCCCGGCTT CCACAGAAAC TCGGTTGCAG 
GCAGTAGACC CAGGCCTGCC AGCAGTGAAG CAAGAGCCAC CTGACCCTGA GGAGGACAAG 
GACGACAACA AGGCTGACTC CACCTCTGAC TTGCCCCCAG AGGAGGAGGC AGGAGGGGCT 
GGCACGCCCG TGATCACGGA GATTTTCAGC CTGGGTGGAA CCCGCCTCCG GGACACAGCA 
GTCTGGTTGC CAAGGTCCAA GGACCTTAAA AAACCTGGAG GTAGAAAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_010818929.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817231.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa54710
Clone ID Related Accession (Same CDS sequence)	XM_010818929.1 , XM_010818929.2
Accession Version	XM_010818929.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCAGCCTTA CACAAACCGT CGGCAGAACC GCAAGTGTGG GACCTGTGCA 
GCCTGCCTGC GGCGGATGGA CTGTGGCCAC TGCGACTTCT GCTGTGACAA GCCCAAGTTT 
GGGGGCAGCA ACCAGAAGCG CCAGAAGTGT CGCTGGCGCC AGTGCCTGCA GTTTGCTATG 
AAGCGGCTGC TGCCAAGTGT CTGGGCAGGG TCCGAAGATG GGGCATCGCC GCCCCCAGCT 
CACCCTCGTC GAAAGAGGCC TGGCTCTACT CGAAGGCCCC ATCTGGGTCA GACCCTGAAG 
CCTCCCTTGG CCACACCCGC AGCTCAACCA GACCGAGCCC AGACTCCAGT GAAGGAGGAA 
GCAGGCAGTG GCTTTGTGCT GCCCCCACCT GGCACTGACC TTGTGTTCTT ACGGGAGGGT 
GCAAGCAGTC CCGTGCAGGT GCCTGGCCCA GCCCCGGCTT CCACAGAAAC TCGGTTGCAG 
GCAGTAGACC CAGGCCTGCC AGCAGTGAAG CAAGAGCCAC CTGACCCTGA GGAGGACAAG 
GACGACAACA AGGCTGACTC CACCTCTGAC TTGCCCCCAG AGGAGGAGGC AGGAGGGGCT 
GGCACGCCCG TGATCACGGA GATTTTCAGC CTGGGTGGAA CCCGCCTCCG GGACACAGCA 
GTCTGGTTGC CAAGTCTGCA GGGCAGGCAA TCGGGAAGGG AAGATGGATG TAAAAAGTGG 
GAGACGGAGG ACACACTGGC GCCCATGAGC ACGAGCTGGA AACCACAAGG ATGGCCTGGA 
AGCCATGTCA GCCTCTCACC ACCTCCAACT TCGGTGACGT GGGTGTCCTG CAGAAGAAGC 
TGGTGCCCTT CATCACAGAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817231.1
CDS	190..1953
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPSLQGRQSGREDGCKKWETEDTLAPMSTSWKPQGWPGSHVSLSPPPTSVTWVSCRRSWCPSSQS

Target ORF information:

RefSeq Version	XM_010818929.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818929.1

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCAGCCTTA CACAAACCGT CGGCAGAACC GCAAGTGTGG GACCTGTGCA 
GCCTGCCTGC GGCGGATGGA CTGTGGCCAC TGCGACTTCT GCTGTGACAA GCCCAAGTTT 
GGGGGCAGCA ACCAGAAGCG CCAGAAGTGT CGCTGGCGCC AGTGCCTGCA GTTTGCTATG 
AAGCGGCTGC TGCCAAGTGT CTGGGCAGGG TCCGAAGATG GGGCATCGCC GCCCCCAGCT 
CACCCTCGTC GAAAGAGGCC TGGCTCTACT CGAAGGCCCC ATCTGGGTCA GACCCTGAAG 
CCTCCCTTGG CCACACCCGC AGCTCAACCA GACCGAGCCC AGACTCCAGT GAAGGAGGAA 
GCAGGCAGTG GCTTTGTGCT GCCCCCACCT GGCACTGACC TTGTGTTCTT ACGGGAGGGT 
GCAAGCAGTC CCGTGCAGGT GCCTGGCCCA GCCCCGGCTT CCACAGAAAC TCGGTTGCAG 
GCAGTAGACC CAGGCCTGCC AGCAGTGAAG CAAGAGCCAC CTGACCCTGA GGAGGACAAG 
GACGACAACA AGGCTGACTC CACCTCTGAC TTGCCCCCAG AGGAGGAGGC AGGAGGGGCT 
GGCACGCCCG TGATCACGGA GATTTTCAGC CTGGGTGGAA CCCGCCTCCG GGACACAGCA 
GTCTGGTTGC CAAGTCTGCA GGGCAGGCAA TCGGGAAGGG AAGATGGATG TAAAAAGTGG 
GAGACGGAGG ACACACTGGC GCCCATGAGC ACGAGCTGGA AACCACAAGG ATGGCCTGGA 
AGCCATGTCA GCCTCTCACC ACCTCCAACT TCGGTGACGT GGGTGTCCTG CAGAAGAAGC 
TGGTGCCCTT CATCACAGAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_010818929.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817231.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa54710
Clone ID Related Accession (Same CDS sequence)	XM_010818929.1 , XM_010818929.2
Accession Version	XM_010818929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCAGCCTTA CACAAACCGT CGGCAGAACC GCAAGTGTGG GACCTGTGCA 
GCCTGCCTGC GGCGGATGGA CTGTGGCCAC TGCGACTTCT GCTGTGACAA GCCCAAGTTT 
GGGGGCAGCA ACCAGAAGCG CCAGAAGTGT CGCTGGCGCC AGTGCCTGCA GTTTGCTATG 
AAGCGGCTGC TGCCAAGTGT CTGGGCAGGG TCCGAAGATG GGGCATCGCC GCCCCCAGCT 
CACCCTCGTC GAAAGAGGCC TGGCTCTACT CGAAGGCCCC ATCTGGGTCA GACCCTGAAG 
CCTCCCTTGG CCACACCCGC AGCTCAACCA GACCGAGCCC AGACTCCAGT GAAGGAGGAA 
GCAGGCAGTG GCTTTGTGCT GCCCCCACCT GGCACTGACC TTGTGTTCTT ACGGGAGGGT 
GCAAGCAGTC CCGTGCAGGT GCCTGGCCCA GCCCCGGCTT CCACAGAAAC TCGGTTGCAG 
GCAGTAGACC CAGGCCTGCC AGCAGTGAAG CAAGAGCCAC CTGACCCTGA GGAGGACAAG 
GACGACAACA AGGCTGACTC CACCTCTGAC TTGCCCCCAG AGGAGGAGGC AGGAGGGGCT 
GGCACGCCCG TGATCACGGA GATTTTCAGC CTGGGTGGAA CCCGCCTCCG GGACACAGCA 
GTCTGGTTGC CAAGTCTGCA GGGCAGGCAA TCGGGAAGGG AAGATGGATG TAAAAAGTGG 
GAGACGGAGG ACACACTGGC GCCCATGAGC ACGAGCTGGA AACCACAAGG ATGGCCTGGA 
AGCCATGTCA GCCTCTCACC ACCTCCAACT TCGGTGACGT GGGTGTCCTG CAGAAGAAGC 
TGGTGCCCTT CATCACAGAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817231.1
CDS	190..1953
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPSLQGRQSGREDGCKKWETEDTLAPMSTSWKPQGWPGSHVSLSPPPTSVTWVSCRRSWCPSSQS

Target ORF information:

RefSeq Version	XM_010818929.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818929.2

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCAGCCTTA CACAAACCGT CGGCAGAACC GCAAGTGTGG GACCTGTGCA 
GCCTGCCTGC GGCGGATGGA CTGTGGCCAC TGCGACTTCT GCTGTGACAA GCCCAAGTTT 
GGGGGCAGCA ACCAGAAGCG CCAGAAGTGT CGCTGGCGCC AGTGCCTGCA GTTTGCTATG 
AAGCGGCTGC TGCCAAGTGT CTGGGCAGGG TCCGAAGATG GGGCATCGCC GCCCCCAGCT 
CACCCTCGTC GAAAGAGGCC TGGCTCTACT CGAAGGCCCC ATCTGGGTCA GACCCTGAAG 
CCTCCCTTGG CCACACCCGC AGCTCAACCA GACCGAGCCC AGACTCCAGT GAAGGAGGAA 
GCAGGCAGTG GCTTTGTGCT GCCCCCACCT GGCACTGACC TTGTGTTCTT ACGGGAGGGT 
GCAAGCAGTC CCGTGCAGGT GCCTGGCCCA GCCCCGGCTT CCACAGAAAC TCGGTTGCAG 
GCAGTAGACC CAGGCCTGCC AGCAGTGAAG CAAGAGCCAC CTGACCCTGA GGAGGACAAG 
GACGACAACA AGGCTGACTC CACCTCTGAC TTGCCCCCAG AGGAGGAGGC AGGAGGGGCT 
GGCACGCCCG TGATCACGGA GATTTTCAGC CTGGGTGGAA CCCGCCTCCG GGACACAGCA 
GTCTGGTTGC CAAGTCTGCA GGGCAGGCAA TCGGGAAGGG AAGATGGATG TAAAAAGTGG 
GAGACGGAGG ACACACTGGC GCCCATGAGC ACGAGCTGGA AACCACAAGG ATGGCCTGGA 
AGCCATGTCA GCCTCTCACC ACCTCCAACT TCGGTGACGT GGGTGTCCTG CAGAAGAAGC 
TGGTGCCCTT CATCACAGAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_010818930.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817232.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa54711
Clone ID Related Accession (Same CDS sequence)	XM_010818930.1 , XM_010818930.2
Accession Version	XM_010818930.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG CAGTAGACCC AGGCCTGCCA GCAGTGAAGC AAGAGCCACC TGACCCTGAG 
GAGGACAAGG ACGACAACAA GGCTGACTCC ACCTCTGACT TGCCCCCAGA GGAGGAGGCA 
GGAGGGGCTG GCACGCCCGT GATCACGGAG ATTTTCAGCC TGGGTGGAAC CCGCCTCCGG 
GACACAGCAG TCTGGTTGCC AAGGGCAGGC AATCGGGAAG GGAAGATGGA TGTAAAAAGT 
GGGAGACGGA GGACACACTG GCGCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817232.1
CDS	190..1899
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPRAGNREGKMDVKSGRRRTHWRP

Target ORF information:

RefSeq Version	XM_010818930.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818930.1

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG CAGTAGACCC AGGCCTGCCA GCAGTGAAGC AAGAGCCACC TGACCCTGAG 
GAGGACAAGG ACGACAACAA GGCTGACTCC ACCTCTGACT TGCCCCCAGA GGAGGAGGCA 
GGAGGGGCTG GCACGCCCGT GATCACGGAG ATTTTCAGCC TGGGTGGAAC CCGCCTCCGG 
GACACAGCAG TCTGGTTGCC AAGGGCAGGC AATCGGGAAG GGAAGATGGA TGTAAAAAGT 
GGGAGACGGA GGACACACTG GCGCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_010818930.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817232.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa54711
Clone ID Related Accession (Same CDS sequence)	XM_010818930.1 , XM_010818930.2
Accession Version	XM_010818930.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818930.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG CAGTAGACCC AGGCCTGCCA GCAGTGAAGC AAGAGCCACC TGACCCTGAG 
GAGGACAAGG ACGACAACAA GGCTGACTCC ACCTCTGACT TGCCCCCAGA GGAGGAGGCA 
GGAGGGGCTG GCACGCCCGT GATCACGGAG ATTTTCAGCC TGGGTGGAAC CCGCCTCCGG 
GACACAGCAG TCTGGTTGCC AAGGGCAGGC AATCGGGAAG GGAAGATGGA TGTAAAAAGT 
GGGAGACGGA GGACACACTG GCGCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817232.1
CDS	190..1899
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPRAGNREGKMDVKSGRRRTHWRP

Target ORF information:

RefSeq Version	XM_010818930.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818930.2

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG CAGTAGACCC AGGCCTGCCA GCAGTGAAGC AAGAGCCACC TGACCCTGAG 
GAGGACAAGG ACGACAACAA GGCTGACTCC ACCTCTGACT TGCCCCCAGA GGAGGAGGCA 
GGAGGGGCTG GCACGCCCGT GATCACGGAG ATTTTCAGCC TGGGTGGAAC CCGCCTCCGG 
GACACAGCAG TCTGGTTGCC AAGGGCAGGC AATCGGGAAG GGAAGATGGA TGTAAAAAGT 
GGGAGACGGA GGACACACTG GCGCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_010818931.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817233.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa54712
Clone ID Related Accession (Same CDS sequence)	XM_010818931.1 , XM_010818931.2
Accession Version	XM_010818931.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG CAGTAGACCC AGGCCTGCCA GCAGTGAAGC AAGAGCCACC TGACCCTGAG 
GAGGACAAGG ACGACAACAA GGCTGACTCC ACCTCTGACT TGCCCCCAGA GGAGGAGGCA 
GGAGGGGCTG GCACGCCCGT GATCACGGAG ATTTTCAGCC TGGGTGGAAC CCGCCTCCGG 
GACACAGCAG TCTGGTTGCC AAGGTCCAAG GACCTTAAAA AACCTGGAGG TAGAAAGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817233.1
CDS	190..1872
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPRSKDLKKPGGRKQ

Target ORF information:

RefSeq Version	XM_010818931.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818931.1

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG CAGTAGACCC AGGCCTGCCA GCAGTGAAGC AAGAGCCACC TGACCCTGAG 
GAGGACAAGG ACGACAACAA GGCTGACTCC ACCTCTGACT TGCCCCCAGA GGAGGAGGCA 
GGAGGGGCTG GCACGCCCGT GATCACGGAG ATTTTCAGCC TGGGTGGAAC CCGCCTCCGG 
GACACAGCAG TCTGGTTGCC AAGGTCCAAG GACCTTAAAA AACCTGGAGG TAGAAAGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_010818931.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817233.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa54712
Clone ID Related Accession (Same CDS sequence)	XM_010818931.1 , XM_010818931.2
Accession Version	XM_010818931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG CAGTAGACCC AGGCCTGCCA GCAGTGAAGC AAGAGCCACC TGACCCTGAG 
GAGGACAAGG ACGACAACAA GGCTGACTCC ACCTCTGACT TGCCCCCAGA GGAGGAGGCA 
GGAGGGGCTG GCACGCCCGT GATCACGGAG ATTTTCAGCC TGGGTGGAAC CCGCCTCCGG 
GACACAGCAG TCTGGTTGCC AAGGTCCAAG GACCTTAAAA AACCTGGAGG TAGAAAGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817233.1
CDS	190..1872
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPRSKDLKKPGGRKQ

Target ORF information:

RefSeq Version	XM_010818931.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818931.2

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG CAGTAGACCC AGGCCTGCCA GCAGTGAAGC AAGAGCCACC TGACCCTGAG 
GAGGACAAGG ACGACAACAA GGCTGACTCC ACCTCTGACT TGCCCCCAGA GGAGGAGGCA 
GGAGGGGCTG GCACGCCCGT GATCACGGAG ATTTTCAGCC TGGGTGGAAC CCGCCTCCGG 
GACACAGCAG TCTGGTTGCC AAGGTCCAAG GACCTTAAAA AACCTGGAGG TAGAAAGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_024984374.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840142.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa229973
Clone ID Related Accession (Same CDS sequence)	XM_024984374.1
Accession Version	XM_024984374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGCACCTTGC TCACCGCCTC CGTCGCCACC ATCAGCGATG TCAACGACGC 
CCTCCCCTGG CTGTGGCTCC CCCTGCTGGT AAACACGGCC GCCGCAGAGG AGGCTGCGAC 
TCCAAGGTGG TGCCCCGGCG GCGTCCACCC CGAGCCCAGT CACCGCCTCC ACCTCCCCCA 
CCGCAGCCTC CAGAGTCTCC GGAGCTGCAC CCCAGAGCCC TGGCCCCCTC GCCACCTGCT 
GAATTCATCT ATTACTGTGT AGACGAGGAC GAGCTACAGC CTTACACAAA CCGTCGGCAG 
AACCGCAAGT GTGGGACCTG TGCAGCCTGC CTGCGGCGGA TGGACTGTGG CCACTGCGAC 
TTCTGCTGTG ACAAGCCCAA GTTTGGGGGC AGCAACCAGA AGCGCCAGAA GTGTCGCTGG 
CGCCAGTGCC TGCAGTTTGC TATGAAGCGG CTGCTGCCAA GTGTCTGGGC AGGGTCCGAA 
GATGGGGCAT CGCCGCCCCC AGCTCACCCT CGTCGAAAGA GGCCTGGCTC TACTCGAAGG 
CCCCATCTGG GTCAGACCCT GAAGCCTCCC TTGGCCACAC CCGCAGCTCA ACCAGACCGA 
GCCCAGACTC CAGTGAAGGA GGAAGCAGGC AGTGGCTTTG TGCTGCCCCC ACCTGGCACT 
GACCTTGTGT TCTTACGGGA GGGTGCAAGC AGTCCCGTGC AGGTGCCTGG CCCAGCCCCG 
GCTTCCACAG AAACTCGGTT GCAGGAGGCC CAGTGCCCTG GCCTGAGTTG GGTTGTGGCC 
TTACCCCAGG TGAAGCAAGA GAAGGCGGAT GCCCAGGAAG ACTGGACACC TGGCACAGCC 
ATCCTGACTT CTCCTGTATT GCTGCCTGGC TGCCCCAGCA AGGCAGTAGA CCCAGGCCTG 
CCAGCAGTGA AGCAAGAGCC ACCTGACCCT GAGGAGGACA AGGACGACAA CAAGGCTGAC 
TCCACCTCTG ACTTGCCCCC AGAGGAGGAG GCAGGAGGGG CTGGCACGCC CGTGATCACG 
GAGATTTTCA GCCTGGGTGG AACCCGCCTC CGGGACACAG CAGTCTGGTT GCCAAGTCTG 
CAGGGCAGGC AATCGGGAAG GGAAGATGGA TGTAAAAAGT GGGAGACGGA GGACACACTG 
GCGCCCATGA GCACGAGCTG GAAACCACAA GGATGGCCTG GAAGCCATGT CAGCCTCTCA 
CCACCTCCAA CTTCGGTGAC GTGGGTGTCC TGCAGAAGAA GCTGGTGCCC TTCATCACAG 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840142.1
CDS	190..2235
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRHLAHRLRRHHQRCQRRPPLAVAPPAGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQEAQCPGLSWVVALPQVKQEKADAQEDWTPGTAILTSPVLLPGCPSKAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPSLQGRQSGREDGCKKWETEDTLAPMSTSWKPQGWPGSHVSLSPPPTSVTWVSCRRSWCPSSQS

Target ORF information:

RefSeq Version	XM_024984374.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984374.1

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGCACCTTGC TCACCGCCTC CGTCGCCACC ATCAGCGATG TCAACGACGC 
CCTCCCCTGG CTGTGGCTCC CCCTGCTGGT AAACACGGCC GCCGCAGAGG AGGCTGCGAC 
TCCAAGGTGG TGCCCCGGCG GCGTCCACCC CGAGCCCAGT CACCGCCTCC ACCTCCCCCA 
CCGCAGCCTC CAGAGTCTCC GGAGCTGCAC CCCAGAGCCC TGGCCCCCTC GCCACCTGCT 
GAATTCATCT ATTACTGTGT AGACGAGGAC GAGCTACAGC CTTACACAAA CCGTCGGCAG 
AACCGCAAGT GTGGGACCTG TGCAGCCTGC CTGCGGCGGA TGGACTGTGG CCACTGCGAC 
TTCTGCTGTG ACAAGCCCAA GTTTGGGGGC AGCAACCAGA AGCGCCAGAA GTGTCGCTGG 
CGCCAGTGCC TGCAGTTTGC TATGAAGCGG CTGCTGCCAA GTGTCTGGGC AGGGTCCGAA 
GATGGGGCAT CGCCGCCCCC AGCTCACCCT CGTCGAAAGA GGCCTGGCTC TACTCGAAGG 
CCCCATCTGG GTCAGACCCT GAAGCCTCCC TTGGCCACAC CCGCAGCTCA ACCAGACCGA 
GCCCAGACTC CAGTGAAGGA GGAAGCAGGC AGTGGCTTTG TGCTGCCCCC ACCTGGCACT 
GACCTTGTGT TCTTACGGGA GGGTGCAAGC AGTCCCGTGC AGGTGCCTGG CCCAGCCCCG 
GCTTCCACAG AAACTCGGTT GCAGGAGGCC CAGTGCCCTG GCCTGAGTTG GGTTGTGGCC 
TTACCCCAGG TGAAGCAAGA GAAGGCGGAT GCCCAGGAAG ACTGGACACC TGGCACAGCC 
ATCCTGACTT CTCCTGTATT GCTGCCTGGC TGCCCCAGCA AGGCAGTAGA CCCAGGCCTG 
CCAGCAGTGA AGCAAGAGCC ACCTGACCCT GAGGAGGACA AGGACGACAA CAAGGCTGAC 
TCCACCTCTG ACTTGCCCCC AGAGGAGGAG GCAGGAGGGG CTGGCACGCC CGTGATCACG 
GAGATTTTCA GCCTGGGTGG AACCCGCCTC CGGGACACAG CAGTCTGGTT GCCAAGTCTG 
CAGGGCAGGC AATCGGGAAG GGAAGATGGA TGTAAAAAGT GGGAGACGGA GGACACACTG 
GCGCCCATGA GCACGAGCTG GAAACCACAA GGATGGCCTG GAAGCCATGT CAGCCTCTCA 
CCACCTCCAA CTTCGGTGAC GTGGGTGTCC TGCAGAAGAA GCTGGTGCCC TTCATCACAG 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_024984375.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840143.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa229974
Clone ID Related Accession (Same CDS sequence)	XM_024984375.1
Accession Version	XM_024984375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGCACCTTGC TCACCGCCTC CGTCGCCACC ATCAGCGATG TCAACGACGC 
CCTCCCCTGG CTGTGGCTCC CCCTGCTGGT AAACACGGCC GCCGCAGAGG AGGCTGCGAC 
TCCAAGGTGG TGCCCCGGCG GCGTCCACCC CGAGCCCAGT CACCGCCTCC ACCTCCCCCA 
CCGCAGCCTC CAGAGTCTCC GGAGCTGCAC CCCAGAGCCC TGGCCCCCTC GCCACCTGCT 
GAATTCATCT ATTACTGTGT AGACGAGGAC GAGCTACAGC CTTACACAAA CCGTCGGCAG 
AACCGCAAGT GTGGGACCTG TGCAGCCTGC CTGCGGCGGA TGGACTGTGG CCACTGCGAC 
TTCTGCTGTG ACAAGCCCAA GTTTGGGGGC AGCAACCAGA AGCGCCAGAA GTGTCGCTGG 
CGCCAGTGCC TGCAGTTTGC TATGAAGCGG CTGCTGCCAA GTGTCTGGGC AGGGTCCGAA 
GATGGGGCAT CGCCGCCCCC AGCTCACCCT CGTCGAAAGA GGCCTGGCTC TACTCGAAGG 
CCCCATCTGG GTCAGACCCT GAAGCCTCCC TTGGCCACAC CCGCAGCTCA ACCAGACCGA 
GCCCAGACTC CAGTGAAGGA GGAAGCAGGC AGTGGCTTTG TGCTGCCCCC ACCTGGCACT 
GACCTTGTGT TCTTACGGGA GGGTGCAAGC AGTCCCGTGC AGGTGCCTGG CCCAGCCCCG 
GCTTCCACAG AAACTCGGTT GCAGGTGAAG CAAGAGAAGG CGGATGCCCA GGAAGACTGG 
ACACCTGGCA CAGCCATCCT GACTTCTCCT GTATTGCTGC CTGGCTGCCC CAGCAAGGCA 
GTAGACCCAG GCCTGCCAGC AGTGAAGCAA GAGCCACCTG ACCCTGAGGA GGACAAGGAC 
GACAACAAGG CTGACTCCAC CTCTGACTTG CCCCCAGAGG AGGAGGCAGG AGGGGCTGGC 
ACGCCCGTGA TCACGGAGAT TTTCAGCCTG GGTGGAACCC GCCTCCGGGA CACAGCAGTC 
TGGTTGCCAA GTCTGCAGGG CAGGCAATCG GGAAGGGAAG ATGGATGTAA AAAGTGGGAG 
ACGGAGGACA CACTGGCGCC CATGAGCACG AGCTGGAAAC CACAAGGATG GCCTGGAAGC 
CATGTCAGCC TCTCACCACC TCCAACTTCG GTGACGTGGG TGTCCTGCAG AAGAAGCTGG 
TGCCCTTCAT CACAGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840143.1
CDS	190..2190
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRHLAHRLRRHHQRCQRRPPLAVAPPAGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQVKQEKADAQEDWTPGTAILTSPVLLPGCPSKAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPSLQGRQSGREDGCKKWETEDTLAPMSTSWKPQGWPGSHVSLSPPPTSVTWVSCRRSWCPSSQS

Target ORF information:

RefSeq Version	XM_024984375.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984375.1

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGCACCTTGC TCACCGCCTC CGTCGCCACC ATCAGCGATG TCAACGACGC 
CCTCCCCTGG CTGTGGCTCC CCCTGCTGGT AAACACGGCC GCCGCAGAGG AGGCTGCGAC 
TCCAAGGTGG TGCCCCGGCG GCGTCCACCC CGAGCCCAGT CACCGCCTCC ACCTCCCCCA 
CCGCAGCCTC CAGAGTCTCC GGAGCTGCAC CCCAGAGCCC TGGCCCCCTC GCCACCTGCT 
GAATTCATCT ATTACTGTGT AGACGAGGAC GAGCTACAGC CTTACACAAA CCGTCGGCAG 
AACCGCAAGT GTGGGACCTG TGCAGCCTGC CTGCGGCGGA TGGACTGTGG CCACTGCGAC 
TTCTGCTGTG ACAAGCCCAA GTTTGGGGGC AGCAACCAGA AGCGCCAGAA GTGTCGCTGG 
CGCCAGTGCC TGCAGTTTGC TATGAAGCGG CTGCTGCCAA GTGTCTGGGC AGGGTCCGAA 
GATGGGGCAT CGCCGCCCCC AGCTCACCCT CGTCGAAAGA GGCCTGGCTC TACTCGAAGG 
CCCCATCTGG GTCAGACCCT GAAGCCTCCC TTGGCCACAC CCGCAGCTCA ACCAGACCGA 
GCCCAGACTC CAGTGAAGGA GGAAGCAGGC AGTGGCTTTG TGCTGCCCCC ACCTGGCACT 
GACCTTGTGT TCTTACGGGA GGGTGCAAGC AGTCCCGTGC AGGTGCCTGG CCCAGCCCCG 
GCTTCCACAG AAACTCGGTT GCAGGTGAAG CAAGAGAAGG CGGATGCCCA GGAAGACTGG 
ACACCTGGCA CAGCCATCCT GACTTCTCCT GTATTGCTGC CTGGCTGCCC CAGCAAGGCA 
GTAGACCCAG GCCTGCCAGC AGTGAAGCAA GAGCCACCTG ACCCTGAGGA GGACAAGGAC 
GACAACAAGG CTGACTCCAC CTCTGACTTG CCCCCAGAGG AGGAGGCAGG AGGGGCTGGC 
ACGCCCGTGA TCACGGAGAT TTTCAGCCTG GGTGGAACCC GCCTCCGGGA CACAGCAGTC 
TGGTTGCCAA GTCTGCAGGG CAGGCAATCG GGAAGGGAAG ATGGATGTAA AAAGTGGGAG 
ACGGAGGACA CACTGGCGCC CATGAGCACG AGCTGGAAAC CACAAGGATG GCCTGGAAGC 
CATGTCAGCC TCTCACCACC TCCAACTTCG GTGACGTGGG TGTCCTGCAG AAGAAGCTGG 
TGCCCTTCAT CACAGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_024984376.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840144.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa229975
Clone ID Related Accession (Same CDS sequence)	XM_024984376.1
Accession Version	XM_024984376.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACCTTACACA AACCGTCGGC AGAACCGCAA GTGTGGGACC 
TGTGCAGCCT GCCTGCGGCG GATGGACTGT GGCCACTGCG ACTTCTGCTG TGACAAGCCC 
AAGTTTGGGG GCAGCAACCA GAAGCGCCAG AAGTGTCGCT GGCGCCAGTG CCTGCAGTTT 
GCTATGAAGC GGCTGCTGCC AAGTGTCTGG GCAGGGTCCG AAGATGGGGC ATCGCCGCCC 
CCAGCTCACC CTCGTCGAAA GAGGCCTGGC TCTACTCGAA GGCCCCATCT GGGTCAGACC 
CTGAAGCCTC CCTTGGCCAC ACCCGCAGCT CAACCAGACC GAGCCCAGAC TCCAGTGAAG 
GAGGAAGCAG GCAGTGGCTT TGTGCTGCCC CCACCTGGCA CTGACCTTGT GTTCTTACGG 
GAGGGTGCAA GCAGTCCCGT GCAGGTGCCT GGCCCAGCCC CGGCTTCCAC AGAAACTCGG 
TTGCAGGAGG CCCAGTGCCC TGGCCTGAGT TGGGTTGTGG CCTTACCCCA GGTGAAGCAA 
GAGAAGGCGG ATGCCCAGGA AGACTGGACA CCTGGCACAG CCATCCTGAC TTCTCCTGTA 
TTGCTGCCTG GCTGCCCCAG CAAGGCAGTA GACCCAGGCC TGCCAGCAGT GAAGCAAGAG 
CCACCTGACC CTGAGGAGGA CAAGGACGAC AACAAGGCTG ACTCCACCTC TGACTTGCCC 
CCAGAGGAGG AGGCAGGAGG GGCTGGCACG CCCGTGATCA CGGAGATTTT CAGCCTGGGT 
GGAACCCGCC TCCGGGACAC AGCAGTCTGG TTGCCAAGGT CCAAGGACCT TAAAAAACCT 
GGAGGTAGAA AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840144.1
CDS	190..2007
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQEAQCPGLSWVVALPQVKQEKADAQEDWTPGTAILTSPVLLPGCPSKAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPRSKDLKKPGGRKQ

Target ORF information:

RefSeq Version	XM_024984376.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984376.1

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACCTTACACA AACCGTCGGC AGAACCGCAA GTGTGGGACC 
TGTGCAGCCT GCCTGCGGCG GATGGACTGT GGCCACTGCG ACTTCTGCTG TGACAAGCCC 
AAGTTTGGGG GCAGCAACCA GAAGCGCCAG AAGTGTCGCT GGCGCCAGTG CCTGCAGTTT 
GCTATGAAGC GGCTGCTGCC AAGTGTCTGG GCAGGGTCCG AAGATGGGGC ATCGCCGCCC 
CCAGCTCACC CTCGTCGAAA GAGGCCTGGC TCTACTCGAA GGCCCCATCT GGGTCAGACC 
CTGAAGCCTC CCTTGGCCAC ACCCGCAGCT CAACCAGACC GAGCCCAGAC TCCAGTGAAG 
GAGGAAGCAG GCAGTGGCTT TGTGCTGCCC CCACCTGGCA CTGACCTTGT GTTCTTACGG 
GAGGGTGCAA GCAGTCCCGT GCAGGTGCCT GGCCCAGCCC CGGCTTCCAC AGAAACTCGG 
TTGCAGGAGG CCCAGTGCCC TGGCCTGAGT TGGGTTGTGG CCTTACCCCA GGTGAAGCAA 
GAGAAGGCGG ATGCCCAGGA AGACTGGACA CCTGGCACAG CCATCCTGAC TTCTCCTGTA 
TTGCTGCCTG GCTGCCCCAG CAAGGCAGTA GACCCAGGCC TGCCAGCAGT GAAGCAAGAG 
CCACCTGACC CTGAGGAGGA CAAGGACGAC AACAAGGCTG ACTCCACCTC TGACTTGCCC 
CCAGAGGAGG AGGCAGGAGG GGCTGGCACG CCCGTGATCA CGGAGATTTT CAGCCTGGGT 
GGAACCCGCC TCCGGGACAC AGCAGTCTGG TTGCCAAGGT CCAAGGACCT TAAAAAACCT 
GGAGGTAGAA AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_024984377.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840145.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa229976
Clone ID Related Accession (Same CDS sequence)	XM_024984377.1
Accession Version	XM_024984377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG TGAAGCAAGA GAAGGCGGAT GCCCAGGAAG ACTGGACACC TGGCACAGCC 
ATCCTGACTT CTCCTGTATT GCTGCCTGGC TGCCCCAGCA AGGCAGTAGA CCCAGGCCTG 
CCAGCAGTGA AGCAAGAGCC ACCTGACCCT GAGGAGGACA AGGACGACAA CAAGGCTGAC 
TCCACCTCTG ACTTGCCCCC AGAGGAGGAG GCAGGAGGGG CTGGCACGCC CGTGATCACG 
GAGATTTTCA GCCTGGGTGG AACCCGCCTC CGGGACACAG CAGTCTGGTT GCCAAGGTCC 
AAGGACCTTA AAAAACCTGG AGGTAGAAAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840145.1
CDS	190..1965
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQVKQEKADAQEDWTPGTAILTSPVLLPGCPSKAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPRSKDLKKPGGRKQ

Target ORF information:

RefSeq Version	XM_024984377.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984377.1

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACAGCCTTAC ACAAACCGTC GGCAGAACCG CAAGTGTGGG 
ACCTGTGCAG CCTGCCTGCG GCGGATGGAC TGTGGCCACT GCGACTTCTG CTGTGACAAG 
CCCAAGTTTG GGGGCAGCAA CCAGAAGCGC CAGAAGTGTC GCTGGCGCCA GTGCCTGCAG 
TTTGCTATGA AGCGGCTGCT GCCAAGTGTC TGGGCAGGGT CCGAAGATGG GGCATCGCCG 
CCCCCAGCTC ACCCTCGTCG AAAGAGGCCT GGCTCTACTC GAAGGCCCCA TCTGGGTCAG 
ACCCTGAAGC CTCCCTTGGC CACACCCGCA GCTCAACCAG ACCGAGCCCA GACTCCAGTG 
AAGGAGGAAG CAGGCAGTGG CTTTGTGCTG CCCCCACCTG GCACTGACCT TGTGTTCTTA 
CGGGAGGGTG CAAGCAGTCC CGTGCAGGTG CCTGGCCCAG CCCCGGCTTC CACAGAAACT 
CGGTTGCAGG TGAAGCAAGA GAAGGCGGAT GCCCAGGAAG ACTGGACACC TGGCACAGCC 
ATCCTGACTT CTCCTGTATT GCTGCCTGGC TGCCCCAGCA AGGCAGTAGA CCCAGGCCTG 
CCAGCAGTGA AGCAAGAGCC ACCTGACCCT GAGGAGGACA AGGACGACAA CAAGGCTGAC 
TCCACCTCTG ACTTGCCCCC AGAGGAGGAG GCAGGAGGGG CTGGCACGCC CGTGATCACG 
GAGATTTTCA GCCTGGGTGG AACCCGCCTC CGGGACACAG CAGTCTGGTT GCCAAGGTCC 
AAGGACCTTA AAAAACCTGG AGGTAGAAAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MBD1 ( XM_024984378.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840146.1 Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X9, mRNA.

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CloneID	OBa229977
Clone ID Related Accession (Same CDS sequence)	XM_024984378.1
Accession Version	XM_024984378.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methyl-CpG-binding domain protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACCTTACACA AACCGTCGGC AGAACCGCAA GTGTGGGACC 
TGTGCAGCCT GCCTGCGGCG GATGGACTGT GGCCACTGCG ACTTCTGCTG TGACAAGCCC 
AAGTTTGGGG GCAGCAACCA GAAGCGCCAG AAGTGTCGCT GGCGCCAGTG CCTGCAGTTT 
GCTATGAAGC GGCTGCTGCC AAGTGTCTGG GCAGGGTCCG AAGATGGGGC ATCGCCGCCC 
CCAGCTCACC CTCGTCGAAA GAGGCCTGGC TCTACTCGAA GGCCCCATCT GGGTCAGACC 
CTGAAGCCTC CCTTGGCCAC ACCCGCAGCT CAACCAGACC GAGCCCAGAC TCCAGTGAAG 
GAGGAAGCAG GCAGTGGCTT TGTGCTGCCC CCACCTGGCA CTGACCTTGT GTTCTTACGG 
GAGGGTGCAA GCAGTCCCGT GCAGGTGCCT GGCCCAGCCC CGGCTTCCAC AGAAACTCGG 
TTGCAGGTGA AGCAAGAGAA GGCGGATGCC CAGGAAGACT GGACACCTGG CACAGCCATC 
CTGACTTCTC CTGTATTGCT GCCTGGCTGC CCCAGCAAGG CAGTAGACCC AGGCCTGCCA 
GCAGTGAAGC AAGAGCCACC TGACCCTGAG GAGGACAAGG ACGACAACAA GGCTGACTCC 
ACCTCTGACT TGCCCCCAGA GGAGGAGGCA GGAGGGGCTG GCACGCCCGT GATCACGGAG 
ATTTTCAGCC TGGGTGGAAC CCGCCTCCGG GACACAGCAG TCTGGTTGCC AAGGTCCAAG 
GACCTTAAAA AACCTGGAGG TAGAAAGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840146.1
CDS	190..1962
Translation	MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGVLCYPSPKAQSLAITSRKRKKPSKPAKARKCQVGPQKSEVRKEAPRDDTKADTDTVPASLPAPGCCENCGISFSGDGTRRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECTACQVKEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTREDCGRCRVCLRPPRPGLRRQWKCVQRRCLRGKHGRRRGGCDSKVVPRRRPPRAQSPPPPPPPQPPESPELHPRALAPSPPAEFIYYCVDEDELPYTNRRQNRKCGTCAACLRRMDCGHCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWAGSEDGASPPPAHPRRKRPGSTRRPHLGQTLKPPLATPAAQPDRAQTPVKEEAGSGFVLPPPGTDLVFLREGASSPVQVPGPAPASTETRLQVKQEKADAQEDWTPGTAILTSPVLLPGCPSKAVDPGLPAVKQEPPDPEEDKDDNKADSTSDLPPEEEAGGAGTPVITEIFSLGGTRLRDTAVWLPRSKDLKKPGGRKQ

Target ORF information:

RefSeq Version	XM_024984378.1
Organism	Bos taurus(cattle)
Definition	Bos taurus methyl-CpG binding domain protein 1 (MBD1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984378.1

ORF Insert Sequence:

ATGGCTGAGG ACTGGCTGGA CTGCCCAGCC CTGGGCCCTG GCTGGAAGCG CCGTGAGGTT 
TTTCGCAAGT CAGGTGCCAC CTGTGGACGC TCAGACACCT ACTACCAGAG CCCCACAGGA 
GACAGGATCC GAAGCAAAGT TGAGCTGACC CGATACCTGG GCCCTGCATG TGATCTCACC 
CTCTTCGACT TCAAACAAGG CGTCCTCTGC TATCCATCCC CCAAGGCCCA ATCCTTGGCC 
ATCACCAGCA GGAAGCGGAA GAAGCCTTCG AAGCCAGCCA AGGCTCGGAA GTGTCAGGTT 
GGACCTCAGA AGAGTGAGGT CAGGAAGGAG GCACCAAGGG ATGACACCAA GGCTGACACT 
GACACAGTCC CAGCTTCGCT TCCTGCCCCT GGGTGCTGTG AGAACTGTGG AATCAGCTTT 
TCAGGAGATG GTACCCGAAG GCAGCGGCTC AAGACTTTGT GCAAGGACTG CCGAGCACAG 
AGGATCGCTT TCAATCGGGA GCAGAGGATG TTTAAGCGTG TGGGCTGCGG GGAGTGCACG 
GCCTGCCAGG TAAAGGAAGA CTGTGGGGCC TGCTCCACCT GCCTCCTGCA GTTGCCCCAT 
GATGTGGCCT CGGGGCTGTT CTGCAAGTGT GAGCGAAGAC GGTGCCTCCG CATTGTGGAA 
AGGAGCCGAG GGTGTGGAGT GTGCCGGGGC TGTCAGACCC GAGAGGACTG TGGCCGCTGT 
CGAGTCTGCC TTCGCCCTCC GCGCCCCGGT CTCCGGCGCC AGTGGAAGTG CGTCCAGCGG 
CGCTGCCTCC GGGGTAAACA CGGCCGCCGC AGAGGAGGCT GCGACTCCAA GGTGGTGCCC 
CGGCGGCGTC CACCCCGAGC CCAGTCACCG CCTCCACCTC CCCCACCGCA GCCTCCAGAG 
TCTCCGGAGC TGCACCCCAG AGCCCTGGCC CCCTCGCCAC CTGCTGAATT CATCTATTAC 
TGTGTAGACG AGGACGAGCT ACCTTACACA AACCGTCGGC AGAACCGCAA GTGTGGGACC 
TGTGCAGCCT GCCTGCGGCG GATGGACTGT GGCCACTGCG ACTTCTGCTG TGACAAGCCC 
AAGTTTGGGG GCAGCAACCA GAAGCGCCAG AAGTGTCGCT GGCGCCAGTG CCTGCAGTTT 
GCTATGAAGC GGCTGCTGCC AAGTGTCTGG GCAGGGTCCG AAGATGGGGC ATCGCCGCCC 
CCAGCTCACC CTCGTCGAAA GAGGCCTGGC TCTACTCGAA GGCCCCATCT GGGTCAGACC 
CTGAAGCCTC CCTTGGCCAC ACCCGCAGCT CAACCAGACC GAGCCCAGAC TCCAGTGAAG 
GAGGAAGCAG GCAGTGGCTT TGTGCTGCCC CCACCTGGCA CTGACCTTGT GTTCTTACGG 
GAGGGTGCAA GCAGTCCCGT GCAGGTGCCT GGCCCAGCCC CGGCTTCCAC AGAAACTCGG 
TTGCAGGTGA AGCAAGAGAA GGCGGATGCC CAGGAAGACT GGACACCTGG CACAGCCATC 
CTGACTTCTC CTGTATTGCT GCCTGGCTGC CCCAGCAAGG CAGTAGACCC AGGCCTGCCA 
GCAGTGAAGC AAGAGCCACC TGACCCTGAG GAGGACAAGG ACGACAACAA GGCTGACTCC 
ACCTCTGACT TGCCCCCAGA GGAGGAGGCA GGAGGGGCTG GCACGCCCGT GATCACGGAG 
ATTTTCAGCC TGGGTGGAAC CCGCCTCCGG GACACAGCAG TCTGGTTGCC AAGGTCCAAG 
GACCTTAAAA AACCTGGAGG TAGAAAGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Unexpected cross-species contamination in genome sequencing projects.
PeerJ2e675(2014)
Merchant S,Wood DE,Salzberg SL

The genome sequence of taurine cattle: a window to ruminant biology and evolution.
Science (New York, N.Y.)324(5926)522-8(2009 Apr)
,Elsik CG,Tellam RL,Worley KC,Gibbs RA,Muzny DM,Weinstock GM,Adelson DL,Eichler EE,Elnitski L,Guig? R,Hamernik DL,Kappes SM,Lewin HA,Lynn DJ,Nicholas FW,Reymond A,Rijnkels M,Skow LC,Zdobnov EM,Schook L,Womack J,Alioto T,Antonarakis SE,Astashyn A,Chapple CE,Chen HC,Chrast J,C?mara F,Ermolaeva O,Henrichsen CN,Hlavina W,Kapustin Y,Kiryutin B,Kitts P,Kokocinski F,Landrum M,Maglott D,Pruitt K,Sapojnikov V,Searle SM,Solovyev V,Souvorov A,Ucla C,Wyss C,Anzola JM,Gerlach D,Elhaik E,Graur D,Reese JT,Edgar RC,McEwan JC,Payne GM,Raison JM,Junier T,Kriventseva EV,Eyras E,Plass M,Donthu R,Larkin DM,Reecy J,Yang MQ,Chen L,Cheng Z,Chitko-McKown CG,Liu GE,Matukumalli LK,Song J,Zhu B,Bradley DG,Brinkman FS,Lau LP,Whiteside MD,Walker A,Wheeler TT,Casey T,German JB,Lemay DG,Maqbool NJ,Molenaar AJ,Seo S,Stothard P,Baldwin CL,Baxter R,Brinkmeyer-Langford CL,Brown WC,Childers CP,Connelley T,Ellis SA,Fritz K,Glass EJ,Herzig CT,Iivanainen A,Lahmers KK,Bennett AK,Dickens CM,Gilbert JG,Hagen DE,Salih H,Aerts J,Caetano AR,Dalrymple B,Garcia JF,Gill CA,Hiendleder SG,Memili E,Spurlock D,Williams JL,Alexander L,Brownstein MJ,Guan L,Holt RA,Jones SJ,Marra MA,Moore R,Moore SS,Roberts A,Taniguchi M,Waterman RC,Chacko J,Chandrabose MM,Cree A,Dao MD,Dinh HH,Gabisi RA,Hines S,Hume J,Jhangiani SN,Joshi V,Kovar CL,Lewis LR,Liu YS,Lopez J,Morgan MB,Nguyen NB,Okwuonu GO,Ruiz SJ,Santibanez J,Wright RA,Buhay C,Ding Y,Dugan-Rocha S,Herdandez J,Holder M,Sabo A,Egan A,Goodell J,Wilczek-Boney K,Fowler GR,Hitchens ME,Lozado RJ,Moen C,Steffen D,Warren JT,Zhang J,Chiu R,Schein JE,Durbin KJ,Havlak P,Jiang H,Liu Y,Qin X,Ren Y,Shen Y,Song H,Bell SN,Davis C,Johnson AJ,Lee S,Nazareth LV,Patel BM,Pu LL,Vattathil S,Williams RL Jr,Curry S,Hamilton C,Sodergren E,Wheeler DA,Barris W,Bennett GL,Eggen A,Green RD,Harhay GP,Hobbs M,Jann O,Keele JW,Kent MP,Lien S,McKay SD,McWilliam S,Ratnakumar A,Schnabel RD,Smith T,Snelling WM,Sonstegard TS,Stone RT,Sugimoto Y,Takasuga A,Taylor JF,Van Tassell CP,Macneil MD,Abatepaulo AR,Abbey CA,Ahola V,Almeida IG,Amadio AF,Anatriello E,Bahadue SM,Biase FH,Boldt CR,Carroll JA,Carvalho WA,Cervelatti EP,Chacko E,Chapin JE,Cheng Y,Choi J,Colley AJ,de Campos TA,De Donato M,Santos IK,de Oliveira CJ,Deobald H,Devinoy E,Donohue KE,Dovc P,Eberlein A,Fitzsimmons CJ,Franzin AM,Garcia GR,Genini S,Gladney CJ,Grant JR,Greaser ML,Green JA,Hadsell DL,Hakimov HA,Halgren R,Harrow JL,Hart EA,Hastings N,Hernandez M,Hu ZL,Ingham A,Iso-Touru T,Jamis C,Jensen K,Kapetis D,Kerr T,Khalil SS,Khatib H,Kolbehdari D,Kumar CG,Kumar D,Leach R,Lee JC,Li C,Logan KM,Malinverni R,Marques E,Martin WF,Martins NF,Maruyama SR,Mazza R,McLean KL,Medrano JF,Moreno BT,Mor? DD,Muntean CT,Nandakumar HP,Nogueira MF,Olsaker I,Pant SD,Panzitta F,Pastor RC,Poli MA,Poslusny N,Rachagani S,Ranganathan S,Razpet A,Riggs PK,Rincon G,Rodriguez-Osorio N,Rodriguez-Zas SL,Romero NE,Rosenwald A,Sando L,Schmutz SM,Shen L,Sherman L,Southey BR,Lutzow YS,Sweedler JV,Tammen I,Telugu BP,Urbanski JM,Utsunomiya YT,Verschoor CP,Waardenberg AJ,Wang Z,Ward R,Weikard R,Welsh TH Jr,White SN,Wilming LG,Wunderlich KR,Yang J,Zhao FQ

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MBD1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム