SMAD4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMAD4
Entrez Gene ID	540248
Full Name	SMAD family member 4
General protein information	Preferred Names SMAD family member 4 Names mothers against decapentaplegic homolog 4 MAD homolog 4 SMAD 4 mothers against DPP homolog 4 mothers against DPP-like 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024984373.1	XP_024840141.1	mothers against decapentaplegic homolog 4 isoform X1
XM_005224250.2	XP_005224307.1	mothers against decapentaplegic homolog 4 isoform X1
XM_015460109.1	XP_015315595.1	mothers against decapentaplegic homolog 4 isoform X2
NM_001076209.1	NP_001069677.1	mothers against decapentaplegic homolog 4
XM_005224250.3	XP_005224307.1	mothers against decapentaplegic homolog 4 isoform X1

Pathways from BioSystems

Homologies

	SMAD4	XP_001096658.1
Mus musculus (house mouse)	Smad4	NP_032566.2
Drosophila melanogaster (fruit fly)	Med	NP_524610.1
Xenopus tropicalis (tropical clawed frog)	smad4.1	XP_002934485.1
Bos taurus (cattle)	SMAD4	NP_001069677.1
Danio rerio (zebrafish)	zmp:0000000768	NP_001116172.1
Homo sapiens (human)	SMAD4	NP_005350.1
Canis lupus familiaris (dog)	SMAD4	XP_005615451.1
Rattus norvegicus (Norway rat)	Smad4	NP_062148.1
Pan troglodytes (chimpanzee)	SMAD4	XP_001155601.1

Gene Ontology
Bibliography

Related articles in PubMed

Evidence supporting a functional requirement of SMAD4 for bovine preimplantation embryonic development: a potential link to embryotrophic actions of follistatin.
Lee KB, Zhang K, Folger JK, Knott JG, Smith GW
Biology of reproduction91(3)62(2014 Sep)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

ALK5 and Smad4 are involved in TGF-beta1-induced pulmonary endothelial permeability.
Birukova AA, Adyshev D, Gorshkov B, Birukov KG, Verin AD
FEBS letters579(18)4031-7(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest SMAD4 mRNA is increased in oocytes during maturation, is maximal in 2-cell blastocysts, remains elevated through 8-cell stage, and is decreased in remaining ectogenesis; embryotrophic actions of follistatin are SMAD4 dependent.
Title: Evidence supporting a functional requirement of SMAD4 for bovine preimplantation embryonic development: a potential link to embryotrophic actions of follistatin.

ALK5 and Smad4 have roles in TGF-beta1-induced pulmonary endothelial permeability
Title: ALK5 and Smad4 are involved in TGF-beta1-induced pulmonary endothelial permeability.

The following SMAD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMAD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43619	NM_001076209.1 Latest version!	Bos taurus SMAD family member 4 (SMAD4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32607	XM_024984373.1 Latest version!	Bos taurus SMAD family member 4 (SMAD4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32607	XM_005224250.3 Latest version!	Bos taurus SMAD family member 4 (SMAD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa88327	XM_015460109.1 Latest version!	Bos taurus SMAD family member 4 (SMAD4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
OBa32607	XM_005224250.2	Bos taurus SMAD family member 4 (SMAD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMAD4 ( NM_001076209.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001069677.1 Bos taurus SMAD family member 4 (SMAD4), mRNA.

Price & Availability↑

CloneID	OBa43619
Clone ID Related Accession (Same CDS sequence)	NM_001076209.1
Accession Version	NM_001076209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Bos taurus(cattle)
Product	mothers against decapentaplegic homolog 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ494856.1. On or before Oct 4, 2006 this sequence version replaced XM_878237.1, XM_592656.2, XM_878313.1, XM_878353.1, XM_878398.1. ##Evidence-Data-START## Transcript exon combination :: DQ494856.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA 
CATAGTTTGA TGTGCCATAG ACAAGGTGGA GAGAGTGAAA CATTCGCCAA AAGAGCAATT 
GAAAGTTTAG TTAAAAAGCT GAAGGAGAAA AAAGATGAAT TGGATTCTTT AATAACAGCT 
ATAACTACAA ATGGAGCTCA CCCTAGCAAG TGTGTTACCA TACAGAGAAC ATTGGATGGA 
AGGCTTCAGG TGGCTGGTCG CAAGGGATTT CCTCATGTGA TCTATGCCCG TCTCTGGAGG 
TGGCCTGACC TTCACAAAAA TGAACTAAAA CATGTTAAAT ATTGTCAGTA TGCATTTGAC 
TTAAAATGCG ATAGTGTCTG TGTGAATCCA TATCACTATG AACGAGTTGT GTCACCTGGA 
ATTGATCTCT CAGGATTAAC ACTGCAGAGT AATGCTCCAC CAAGTATGTT GGTGAAGGAT 
GAATATGTTC ATGACTTTGA GGGACAGCCA TCATTATCCA CTGAAGGGCA TTCAATTCAA 
ACCATCCAGC ATCCACCAAG TAATCGTGCA TCGACGGAGA CATACAGCAC CCCAGCTCTC 
TTAGCTCCAT CTGAGTCTAA TGCTACCAGC ACCACCAATT TTCCCAACAT TCCTGTGGCT 
TCCACAAGTC AGCCCGCCAG TATACTGGCA GGCAGCCATA GTGAAGGATT GTTGCAGATA 
GCTTCAGGGC CTCAGCCAGG ACAGCAGCAG AATGGATTTA CTGGTCAGCC AGCTACTTAT 
CATCATAACA GCACTACCAC CTGGACGGGA GGTAGGACTG CACCATACAC ACCTAATTTG 
CCTCACCACC AAAACGGCCA TCTCCAGCAC CACCCGCCTA TGCCGCCCCA TCCCGGACAT 
TACTGGCCAC CAGTTCACAA TGAGCTTGCA TTCCAGCCTC CTATTTCTAA TCATCCTGCT 
CCTGAGTATT GGTGTTCCAT TGCTTACTTC GAGATGGATG TTCAAGTAGG AGAGACATTT 
AAGGTTCCTT CAAGCTGTCC TATTGTTACT GTTGATGGAT ATGTAGACCC TTCTGGAGGA 
GATCGCTTTT GCTTGGGTCA ACTCTCCAAT GTCCACAGGA CAGAAGCCAT TGAGAGAGCA 
AGGTTGCACA TAGGCAAAGG TGTGCAGTTG GAATGTAAAG GTGAAGGTGA TGTTTGGGTC 
AGGTGCCTTA GTGACCACGC AGTCTTTGTA CAGAGCTACT ACTTAGACAG AGAAGCTGGG 
CGTGCACCTG GAGATGCTGT TCATAAGATC TACCCAAGTG CATACATAAA GGTCTTTGAT 
TTGCGTCAGT GTCACCGTCA GATGCAGCAG CAGGCAGCCA CGGCACAAGC TGCAGCAGCT 
GCCCAGGCAG CGGCCGTGGC AGGAAACATC CCCGGCCCAG GGTCAGTAGG TGGAATAGCC 
CCAGCCATCA GTTTGTCGGC TGCCGCCGGG ATCGGTGTGG ATGACCTCCG TCGTTTATGC 
ATACTCAGGA TGAGTTTTGT GAAAGGCTGG GGACCTGATT ACCCAAGACA GAGCATCAAG 
GAAACACCTT GTTGGATTGA GATTCACTTA CACCGGGCCC TCCAGCTCCT CGATGAAGTC 
CTTCACACCA TGCCTATTGC CGACCCACAG CCTTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001069677.1
CDS	90..1751
Translation	MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPPSMLVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTTNFPNIPVASTSQPASILAGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGGRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD

Target ORF information:

RefSeq Version	NM_001076209.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD family member 4 (SMAD4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076209.1

ORF Insert Sequence:

ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA 
CATAGTTTGA TGTGCCATAG ACAAGGTGGA GAGAGTGAAA CATTCGCCAA AAGAGCAATT 
GAAAGTTTAG TTAAAAAGCT GAAGGAGAAA AAAGATGAAT TGGATTCTTT AATAACAGCT 
ATAACTACAA ATGGAGCTCA CCCTAGCAAG TGTGTTACCA TACAGAGAAC ATTGGATGGA 
AGGCTTCAGG TGGCTGGTCG CAAGGGATTT CCTCATGTGA TCTATGCCCG TCTCTGGAGG 
TGGCCTGACC TTCACAAAAA TGAACTAAAA CATGTTAAAT ATTGTCAGTA TGCATTTGAC 
TTAAAATGCG ATAGTGTCTG TGTGAATCCA TATCACTATG AACGAGTTGT GTCACCTGGA 
ATTGATCTCT CAGGATTAAC ACTGCAGAGT AATGCTCCAC CAAGTATGTT GGTGAAGGAT 
GAATATGTTC ATGACTTTGA GGGACAGCCA TCATTATCCA CTGAAGGGCA TTCAATTCAA 
ACCATCCAGC ATCCACCAAG TAATCGTGCA TCGACGGAGA CATACAGCAC CCCAGCTCTC 
TTAGCTCCAT CTGAGTCTAA TGCTACCAGC ACCACCAATT TTCCCAACAT TCCTGTGGCT 
TCCACAAGTC AGCCCGCCAG TATACTGGCA GGCAGCCATA GTGAAGGATT GTTGCAGATA 
GCTTCAGGGC CTCAGCCAGG ACAGCAGCAG AATGGATTTA CTGGTCAGCC AGCTACTTAT 
CATCATAACA GCACTACCAC CTGGACGGGA GGTAGGACTG CACCATACAC ACCTAATTTG 
CCTCACCACC AAAACGGCCA TCTCCAGCAC CACCCGCCTA TGCCGCCCCA TCCCGGACAT 
TACTGGCCAC CAGTTCACAA TGAGCTTGCA TTCCAGCCTC CTATTTCTAA TCATCCTGCT 
CCTGAGTATT GGTGTTCCAT TGCTTACTTC GAGATGGATG TTCAAGTAGG AGAGACATTT 
AAGGTTCCTT CAAGCTGTCC TATTGTTACT GTTGATGGAT ATGTAGACCC TTCTGGAGGA 
GATCGCTTTT GCTTGGGTCA ACTCTCCAAT GTCCACAGGA CAGAAGCCAT TGAGAGAGCA 
AGGTTGCACA TAGGCAAAGG TGTGCAGTTG GAATGTAAAG GTGAAGGTGA TGTTTGGGTC 
AGGTGCCTTA GTGACCACGC AGTCTTTGTA CAGAGCTACT ACTTAGACAG AGAAGCTGGG 
CGTGCACCTG GAGATGCTGT TCATAAGATC TACCCAAGTG CATACATAAA GGTCTTTGAT 
TTGCGTCAGT GTCACCGTCA GATGCAGCAG CAGGCAGCCA CGGCACAAGC TGCAGCAGCT 
GCCCAGGCAG CGGCCGTGGC AGGAAACATC CCCGGCCCAG GGTCAGTAGG TGGAATAGCC 
CCAGCCATCA GTTTGTCGGC TGCCGCCGGG ATCGGTGTGG ATGACCTCCG TCGTTTATGC 
ATACTCAGGA TGAGTTTTGT GAAAGGCTGG GGACCTGATT ACCCAAGACA GAGCATCAAG 
GAAACACCTT GTTGGATTGA GATTCACTTA CACCGGGCCC TCCAGCTCCT CGATGAAGTC 
CTTCACACCA TGCCTATTGC CGACCCACAG CCTTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMAD4 ( XM_024984373.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840141.1 Bos taurus SMAD family member 4 (SMAD4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa32607
Clone ID Related Accession (Same CDS sequence)	XM_024984373.1 , XM_005224250.2 , XM_005224250.3
Accession Version	XM_024984373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mothers against decapentaplegic homolog 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA 
CATAGTTTGA TGTGCCATAG ACAAGGTGGA GAGAGTGAAA CATTCGCCAA AAGAGCAATT 
GAAAGTTTAG TTAAAAAGCT GAAGGAGAAA AAAGATGAAT TGGATTCTTT AATAACAGCT 
ATAACTACAA ATGGAGCTCA CCCTAGCAAG TGTGTTACCA TACAGAGAAC ATTGGATGGA 
AGGCTTCAGG TGGCTGGTCG CAAGGGATTT CCTCATGTGA TCTATGCCCG TCTCTGGAGG 
TGGCCTGACC TTCACAAAAA TGAACTAAAA CATGTTAAAT ATTGTCAGTA TGCATTTGAC 
TTAAAATGTG ATAGTGTCTG TGTGAATCCA TATCACTATG AACGAGTTGT GTCACCTGGA 
ATTGATCTCT CAGGATTAAC ACTGCAGAGT AATGCTCCAC CAAGTATGTT GGTGAAGGAT 
GAATATGTTC ATGACTTTGA GGGACAGCCA TCATTATCCA CTGAAGGGCA TTCAATTCAA 
ACCATCCAGC ATCCACCAAG TAATCGTGCA TCGACGGAGA CATACAGCAC CCCAGCTCTC 
TTAGCTCCAT CTGAGTCTAA TGCTACCAGC ACCACCAATT TTCCCAACAT TCCTGTGGCT 
TCCACAAGTC AGCCCGCCAG TATACTGGCA GGCAGCCATA GTGAAGGATT GTTGCAGATA 
GCTTCAGGGC CTCAGCCAGG ACAGCAGCAG AATGGATTTA CTGGTCAGCC AGCTACTTAT 
CATCATAACA GCACTACCAC CTGGACGGGA AGTAGGACTG CACCATACAC ACCTAATTTG 
CCTCACCACC AAAACGGCCA TCTCCAGCAC CACCCGCCTA TGCCGCCCCA TCCCGGACAT 
TACTGGCCAC CAGTTCACAA TGAGCTTGCA TTCCAGCCTC CTATTTCTAA TCATCCTGCT 
CCTGAGTATT GGTGTTCCAT TGCTTACTTC GAGATGGATG TTCAAGTAGG AGAGACATTT 
AAGGTTCCTT CAAGCTGTCC TATTGTTACT GTTGATGGAT ATGTAGACCC TTCTGGAGGA 
GATCGCTTTT GCTTGGGTCA ACTCTCCAAT GTCCACAGGA CAGAAGCCAT TGAGAGAGCA 
AGGTTGCACA TAGGCAAAGG TGTGCAGTTG GAATGTAAAG GTGAAGGTGA TGTTTGGGTC 
AGGTGCCTTA GTGACCACGC AGTCTTTGTA CAGAGCTACT ACTTAGACAG AGAAGCTGGG 
CGTGCACCTG GAGATGCTGT TCATAAGATC TACCCAAGTG CATATATAAA GGTCTTTGAT 
TTGCGTCAGT GTCACCGTCA GATGCAGCAG CAGGCAGCCA CGGCACAAGC TGCAGCAGCT 
GCCCAGGCAG CGGCCGTGGC AGGAAACATC CCCGGCCCAG GGTCAGTAGG TGGAATAGCC 
CCAGCCATCA GTTTGTCGGC TGCCGCCGGG ATCGGTGTGG ATGACCTCCG TCGTTTATGC 
ATACTCAGGA TGAGTTTTGT GAAAGGCTGG GGACCTGATT ACCCAAGACA GAGCATCAAG 
GAAACACCTT GTTGGATTGA GATTCACTTA CACCGGGCCC TCCAGCTCCT CGATGAAGTC 
CTTCACACCA TGCCTATTGC CGACCCACAG CCTTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840141.1
CDS	303..1964
Translation	MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPPSMLVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTTNFPNIPVASTSQPASILAGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD

Target ORF information:

RefSeq Version	XM_024984373.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD family member 4 (SMAD4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984373.1

ORF Insert Sequence:

ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA 
CATAGTTTGA TGTGCCATAG ACAAGGTGGA GAGAGTGAAA CATTCGCCAA AAGAGCAATT 
GAAAGTTTAG TTAAAAAGCT GAAGGAGAAA AAAGATGAAT TGGATTCTTT AATAACAGCT 
ATAACTACAA ATGGAGCTCA CCCTAGCAAG TGTGTTACCA TACAGAGAAC ATTGGATGGA 
AGGCTTCAGG TGGCTGGTCG CAAGGGATTT CCTCATGTGA TCTATGCCCG TCTCTGGAGG 
TGGCCTGACC TTCACAAAAA TGAACTAAAA CATGTTAAAT ATTGTCAGTA TGCATTTGAC 
TTAAAATGTG ATAGTGTCTG TGTGAATCCA TATCACTATG AACGAGTTGT GTCACCTGGA 
ATTGATCTCT CAGGATTAAC ACTGCAGAGT AATGCTCCAC CAAGTATGTT GGTGAAGGAT 
GAATATGTTC ATGACTTTGA GGGACAGCCA TCATTATCCA CTGAAGGGCA TTCAATTCAA 
ACCATCCAGC ATCCACCAAG TAATCGTGCA TCGACGGAGA CATACAGCAC CCCAGCTCTC 
TTAGCTCCAT CTGAGTCTAA TGCTACCAGC ACCACCAATT TTCCCAACAT TCCTGTGGCT 
TCCACAAGTC AGCCCGCCAG TATACTGGCA GGCAGCCATA GTGAAGGATT GTTGCAGATA 
GCTTCAGGGC CTCAGCCAGG ACAGCAGCAG AATGGATTTA CTGGTCAGCC AGCTACTTAT 
CATCATAACA GCACTACCAC CTGGACGGGA AGTAGGACTG CACCATACAC ACCTAATTTG 
CCTCACCACC AAAACGGCCA TCTCCAGCAC CACCCGCCTA TGCCGCCCCA TCCCGGACAT 
TACTGGCCAC CAGTTCACAA TGAGCTTGCA TTCCAGCCTC CTATTTCTAA TCATCCTGCT 
CCTGAGTATT GGTGTTCCAT TGCTTACTTC GAGATGGATG TTCAAGTAGG AGAGACATTT 
AAGGTTCCTT CAAGCTGTCC TATTGTTACT GTTGATGGAT ATGTAGACCC TTCTGGAGGA 
GATCGCTTTT GCTTGGGTCA ACTCTCCAAT GTCCACAGGA CAGAAGCCAT TGAGAGAGCA 
AGGTTGCACA TAGGCAAAGG TGTGCAGTTG GAATGTAAAG GTGAAGGTGA TGTTTGGGTC 
AGGTGCCTTA GTGACCACGC AGTCTTTGTA CAGAGCTACT ACTTAGACAG AGAAGCTGGG 
CGTGCACCTG GAGATGCTGT TCATAAGATC TACCCAAGTG CATATATAAA GGTCTTTGAT 
TTGCGTCAGT GTCACCGTCA GATGCAGCAG CAGGCAGCCA CGGCACAAGC TGCAGCAGCT 
GCCCAGGCAG CGGCCGTGGC AGGAAACATC CCCGGCCCAG GGTCAGTAGG TGGAATAGCC 
CCAGCCATCA GTTTGTCGGC TGCCGCCGGG ATCGGTGTGG ATGACCTCCG TCGTTTATGC 
ATACTCAGGA TGAGTTTTGT GAAAGGCTGG GGACCTGATT ACCCAAGACA GAGCATCAAG 
GAAACACCTT GTTGGATTGA GATTCACTTA CACCGGGCCC TCCAGCTCCT CGATGAAGTC 
CTTCACACCA TGCCTATTGC CGACCCACAG CCTTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMAD4 ( XM_005224250.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224307.1 Bos taurus SMAD family member 4 (SMAD4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32607
Clone ID Related Accession (Same CDS sequence)	XM_024984373.1 , XM_005224250.2 , XM_005224250.3
Accession Version	XM_005224250.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mothers against decapentaplegic homolog 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005224250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA 
CATAGTTTGA TGTGCCATAG ACAAGGTGGA GAGAGTGAAA CATTCGCCAA AAGAGCAATT 
GAAAGTTTAG TTAAAAAGCT GAAGGAGAAA AAAGATGAAT TGGATTCTTT AATAACAGCT 
ATAACTACAA ATGGAGCTCA CCCTAGCAAG TGTGTTACCA TACAGAGAAC ATTGGATGGA 
AGGCTTCAGG TGGCTGGTCG CAAGGGATTT CCTCATGTGA TCTATGCCCG TCTCTGGAGG 
TGGCCTGACC TTCACAAAAA TGAACTAAAA CATGTTAAAT ATTGTCAGTA TGCATTTGAC 
TTAAAATGTG ATAGTGTCTG TGTGAATCCA TATCACTATG AACGAGTTGT GTCACCTGGA 
ATTGATCTCT CAGGATTAAC ACTGCAGAGT AATGCTCCAC CAAGTATGTT GGTGAAGGAT 
GAATATGTTC ATGACTTTGA GGGACAGCCA TCATTATCCA CTGAAGGGCA TTCAATTCAA 
ACCATCCAGC ATCCACCAAG TAATCGTGCA TCGACGGAGA CATACAGCAC CCCAGCTCTC 
TTAGCTCCAT CTGAGTCTAA TGCTACCAGC ACCACCAATT TTCCCAACAT TCCTGTGGCT 
TCCACAAGTC AGCCCGCCAG TATACTGGCA GGCAGCCATA GTGAAGGATT GTTGCAGATA 
GCTTCAGGGC CTCAGCCAGG ACAGCAGCAG AATGGATTTA CTGGTCAGCC AGCTACTTAT 
CATCATAACA GCACTACCAC CTGGACGGGA AGTAGGACTG CACCATACAC ACCTAATTTG 
CCTCACCACC AAAACGGCCA TCTCCAGCAC CACCCGCCTA TGCCGCCCCA TCCCGGACAT 
TACTGGCCAC CAGTTCACAA TGAGCTTGCA TTCCAGCCTC CTATTTCTAA TCATCCTGCT 
CCTGAGTATT GGTGTTCCAT TGCTTACTTC GAGATGGATG TTCAAGTAGG AGAGACATTT 
AAGGTTCCTT CAAGCTGTCC TATTGTTACT GTTGATGGAT ATGTAGACCC TTCTGGAGGA 
GATCGCTTTT GCTTGGGTCA ACTCTCCAAT GTCCACAGGA CAGAAGCCAT TGAGAGAGCA 
AGGTTGCACA TAGGCAAAGG TGTGCAGTTG GAATGTAAAG GTGAAGGTGA TGTTTGGGTC 
AGGTGCCTTA GTGACCACGC AGTCTTTGTA CAGAGCTACT ACTTAGACAG AGAAGCTGGG 
CGTGCACCTG GAGATGCTGT TCATAAGATC TACCCAAGTG CATATATAAA GGTCTTTGAT 
TTGCGTCAGT GTCACCGTCA GATGCAGCAG CAGGCAGCCA CGGCACAAGC TGCAGCAGCT 
GCCCAGGCAG CGGCCGTGGC AGGAAACATC CCCGGCCCAG GGTCAGTAGG TGGAATAGCC 
CCAGCCATCA GTTTGTCGGC TGCCGCCGGG ATCGGTGTGG ATGACCTCCG TCGTTTATGC 
ATACTCAGGA TGAGTTTTGT GAAAGGCTGG GGACCTGATT ACCCAAGACA GAGCATCAAG 
GAAACACCTT GTTGGATTGA GATTCACTTA CACCGGGCCC TCCAGCTCCT CGATGAAGTC 
CTTCACACCA TGCCTATTGC CGACCCACAG CCTTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224307.1
CDS	529..2190
Translation	MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPPSMLVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTTNFPNIPVASTSQPASILAGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD

Target ORF information:

RefSeq Version	XM_005224250.2
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD family member 4 (SMAD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224250.2

ORF Insert Sequence:

ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA 
CATAGTTTGA TGTGCCATAG ACAAGGTGGA GAGAGTGAAA CATTCGCCAA AAGAGCAATT 
GAAAGTTTAG TTAAAAAGCT GAAGGAGAAA AAAGATGAAT TGGATTCTTT AATAACAGCT 
ATAACTACAA ATGGAGCTCA CCCTAGCAAG TGTGTTACCA TACAGAGAAC ATTGGATGGA 
AGGCTTCAGG TGGCTGGTCG CAAGGGATTT CCTCATGTGA TCTATGCCCG TCTCTGGAGG 
TGGCCTGACC TTCACAAAAA TGAACTAAAA CATGTTAAAT ATTGTCAGTA TGCATTTGAC 
TTAAAATGTG ATAGTGTCTG TGTGAATCCA TATCACTATG AACGAGTTGT GTCACCTGGA 
ATTGATCTCT CAGGATTAAC ACTGCAGAGT AATGCTCCAC CAAGTATGTT GGTGAAGGAT 
GAATATGTTC ATGACTTTGA GGGACAGCCA TCATTATCCA CTGAAGGGCA TTCAATTCAA 
ACCATCCAGC ATCCACCAAG TAATCGTGCA TCGACGGAGA CATACAGCAC CCCAGCTCTC 
TTAGCTCCAT CTGAGTCTAA TGCTACCAGC ACCACCAATT TTCCCAACAT TCCTGTGGCT 
TCCACAAGTC AGCCCGCCAG TATACTGGCA GGCAGCCATA GTGAAGGATT GTTGCAGATA 
GCTTCAGGGC CTCAGCCAGG ACAGCAGCAG AATGGATTTA CTGGTCAGCC AGCTACTTAT 
CATCATAACA GCACTACCAC CTGGACGGGA AGTAGGACTG CACCATACAC ACCTAATTTG 
CCTCACCACC AAAACGGCCA TCTCCAGCAC CACCCGCCTA TGCCGCCCCA TCCCGGACAT 
TACTGGCCAC CAGTTCACAA TGAGCTTGCA TTCCAGCCTC CTATTTCTAA TCATCCTGCT 
CCTGAGTATT GGTGTTCCAT TGCTTACTTC GAGATGGATG TTCAAGTAGG AGAGACATTT 
AAGGTTCCTT CAAGCTGTCC TATTGTTACT GTTGATGGAT ATGTAGACCC TTCTGGAGGA 
GATCGCTTTT GCTTGGGTCA ACTCTCCAAT GTCCACAGGA CAGAAGCCAT TGAGAGAGCA 
AGGTTGCACA TAGGCAAAGG TGTGCAGTTG GAATGTAAAG GTGAAGGTGA TGTTTGGGTC 
AGGTGCCTTA GTGACCACGC AGTCTTTGTA CAGAGCTACT ACTTAGACAG AGAAGCTGGG 
CGTGCACCTG GAGATGCTGT TCATAAGATC TACCCAAGTG CATATATAAA GGTCTTTGAT 
TTGCGTCAGT GTCACCGTCA GATGCAGCAG CAGGCAGCCA CGGCACAAGC TGCAGCAGCT 
GCCCAGGCAG CGGCCGTGGC AGGAAACATC CCCGGCCCAG GGTCAGTAGG TGGAATAGCC 
CCAGCCATCA GTTTGTCGGC TGCCGCCGGG ATCGGTGTGG ATGACCTCCG TCGTTTATGC 
ATACTCAGGA TGAGTTTTGT GAAAGGCTGG GGACCTGATT ACCCAAGACA GAGCATCAAG 
GAAACACCTT GTTGGATTGA GATTCACTTA CACCGGGCCC TCCAGCTCCT CGATGAAGTC 
CTTCACACCA TGCCTATTGC CGACCCACAG CCTTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMAD4 ( XM_005224250.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224307.1 Bos taurus SMAD family member 4 (SMAD4), transcript variant X1, mRNA.

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CloneID	OBa32607
Clone ID Related Accession (Same CDS sequence)	XM_024984373.1 , XM_005224250.2 , XM_005224250.3
Accession Version	XM_005224250.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mothers against decapentaplegic homolog 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224250.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA 
CATAGTTTGA TGTGCCATAG ACAAGGTGGA GAGAGTGAAA CATTCGCCAA AAGAGCAATT 
GAAAGTTTAG TTAAAAAGCT GAAGGAGAAA AAAGATGAAT TGGATTCTTT AATAACAGCT 
ATAACTACAA ATGGAGCTCA CCCTAGCAAG TGTGTTACCA TACAGAGAAC ATTGGATGGA 
AGGCTTCAGG TGGCTGGTCG CAAGGGATTT CCTCATGTGA TCTATGCCCG TCTCTGGAGG 
TGGCCTGACC TTCACAAAAA TGAACTAAAA CATGTTAAAT ATTGTCAGTA TGCATTTGAC 
TTAAAATGTG ATAGTGTCTG TGTGAATCCA TATCACTATG AACGAGTTGT GTCACCTGGA 
ATTGATCTCT CAGGATTAAC ACTGCAGAGT AATGCTCCAC CAAGTATGTT GGTGAAGGAT 
GAATATGTTC ATGACTTTGA GGGACAGCCA TCATTATCCA CTGAAGGGCA TTCAATTCAA 
ACCATCCAGC ATCCACCAAG TAATCGTGCA TCGACGGAGA CATACAGCAC CCCAGCTCTC 
TTAGCTCCAT CTGAGTCTAA TGCTACCAGC ACCACCAATT TTCCCAACAT TCCTGTGGCT 
TCCACAAGTC AGCCCGCCAG TATACTGGCA GGCAGCCATA GTGAAGGATT GTTGCAGATA 
GCTTCAGGGC CTCAGCCAGG ACAGCAGCAG AATGGATTTA CTGGTCAGCC AGCTACTTAT 
CATCATAACA GCACTACCAC CTGGACGGGA AGTAGGACTG CACCATACAC ACCTAATTTG 
CCTCACCACC AAAACGGCCA TCTCCAGCAC CACCCGCCTA TGCCGCCCCA TCCCGGACAT 
TACTGGCCAC CAGTTCACAA TGAGCTTGCA TTCCAGCCTC CTATTTCTAA TCATCCTGCT 
CCTGAGTATT GGTGTTCCAT TGCTTACTTC GAGATGGATG TTCAAGTAGG AGAGACATTT 
AAGGTTCCTT CAAGCTGTCC TATTGTTACT GTTGATGGAT ATGTAGACCC TTCTGGAGGA 
GATCGCTTTT GCTTGGGTCA ACTCTCCAAT GTCCACAGGA CAGAAGCCAT TGAGAGAGCA 
AGGTTGCACA TAGGCAAAGG TGTGCAGTTG GAATGTAAAG GTGAAGGTGA TGTTTGGGTC 
AGGTGCCTTA GTGACCACGC AGTCTTTGTA CAGAGCTACT ACTTAGACAG AGAAGCTGGG 
CGTGCACCTG GAGATGCTGT TCATAAGATC TACCCAAGTG CATATATAAA GGTCTTTGAT 
TTGCGTCAGT GTCACCGTCA GATGCAGCAG CAGGCAGCCA CGGCACAAGC TGCAGCAGCT 
GCCCAGGCAG CGGCCGTGGC AGGAAACATC CCCGGCCCAG GGTCAGTAGG TGGAATAGCC 
CCAGCCATCA GTTTGTCGGC TGCCGCCGGG ATCGGTGTGG ATGACCTCCG TCGTTTATGC 
ATACTCAGGA TGAGTTTTGT GAAAGGCTGG GGACCTGATT ACCCAAGACA GAGCATCAAG 
GAAACACCTT GTTGGATTGA GATTCACTTA CACCGGGCCC TCCAGCTCCT CGATGAAGTC 
CTTCACACCA TGCCTATTGC CGACCCACAG CCTTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224307.1
CDS	238..1899
Translation	MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPPSMLVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTTNFPNIPVASTSQPASILAGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD

Target ORF information:

RefSeq Version	XM_005224250.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD family member 4 (SMAD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224250.3

ORF Insert Sequence:

ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA 
CATAGTTTGA TGTGCCATAG ACAAGGTGGA GAGAGTGAAA CATTCGCCAA AAGAGCAATT 
GAAAGTTTAG TTAAAAAGCT GAAGGAGAAA AAAGATGAAT TGGATTCTTT AATAACAGCT 
ATAACTACAA ATGGAGCTCA CCCTAGCAAG TGTGTTACCA TACAGAGAAC ATTGGATGGA 
AGGCTTCAGG TGGCTGGTCG CAAGGGATTT CCTCATGTGA TCTATGCCCG TCTCTGGAGG 
TGGCCTGACC TTCACAAAAA TGAACTAAAA CATGTTAAAT ATTGTCAGTA TGCATTTGAC 
TTAAAATGTG ATAGTGTCTG TGTGAATCCA TATCACTATG AACGAGTTGT GTCACCTGGA 
ATTGATCTCT CAGGATTAAC ACTGCAGAGT AATGCTCCAC CAAGTATGTT GGTGAAGGAT 
GAATATGTTC ATGACTTTGA GGGACAGCCA TCATTATCCA CTGAAGGGCA TTCAATTCAA 
ACCATCCAGC ATCCACCAAG TAATCGTGCA TCGACGGAGA CATACAGCAC CCCAGCTCTC 
TTAGCTCCAT CTGAGTCTAA TGCTACCAGC ACCACCAATT TTCCCAACAT TCCTGTGGCT 
TCCACAAGTC AGCCCGCCAG TATACTGGCA GGCAGCCATA GTGAAGGATT GTTGCAGATA 
GCTTCAGGGC CTCAGCCAGG ACAGCAGCAG AATGGATTTA CTGGTCAGCC AGCTACTTAT 
CATCATAACA GCACTACCAC CTGGACGGGA AGTAGGACTG CACCATACAC ACCTAATTTG 
CCTCACCACC AAAACGGCCA TCTCCAGCAC CACCCGCCTA TGCCGCCCCA TCCCGGACAT 
TACTGGCCAC CAGTTCACAA TGAGCTTGCA TTCCAGCCTC CTATTTCTAA TCATCCTGCT 
CCTGAGTATT GGTGTTCCAT TGCTTACTTC GAGATGGATG TTCAAGTAGG AGAGACATTT 
AAGGTTCCTT CAAGCTGTCC TATTGTTACT GTTGATGGAT ATGTAGACCC TTCTGGAGGA 
GATCGCTTTT GCTTGGGTCA ACTCTCCAAT GTCCACAGGA CAGAAGCCAT TGAGAGAGCA 
AGGTTGCACA TAGGCAAAGG TGTGCAGTTG GAATGTAAAG GTGAAGGTGA TGTTTGGGTC 
AGGTGCCTTA GTGACCACGC AGTCTTTGTA CAGAGCTACT ACTTAGACAG AGAAGCTGGG 
CGTGCACCTG GAGATGCTGT TCATAAGATC TACCCAAGTG CATATATAAA GGTCTTTGAT 
TTGCGTCAGT GTCACCGTCA GATGCAGCAG CAGGCAGCCA CGGCACAAGC TGCAGCAGCT 
GCCCAGGCAG CGGCCGTGGC AGGAAACATC CCCGGCCCAG GGTCAGTAGG TGGAATAGCC 
CCAGCCATCA GTTTGTCGGC TGCCGCCGGG ATCGGTGTGG ATGACCTCCG TCGTTTATGC 
ATACTCAGGA TGAGTTTTGT GAAAGGCTGG GGACCTGATT ACCCAAGACA GAGCATCAAG 
GAAACACCTT GTTGGATTGA GATTCACTTA CACCGGGCCC TCCAGCTCCT CGATGAAGTC 
CTTCACACCA TGCCTATTGC CGACCCACAG CCTTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMAD4 ( XM_015460109.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315595.1 Bos taurus SMAD family member 4 (SMAD4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa88327
Clone ID Related Accession (Same CDS sequence)	XM_015460109.1
Accession Version	XM_015460109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mothers against decapentaplegic homolog 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA 
CATAGTTTGA TGTGCCATAG ACAAGGTGGA GAGAGTGAAA CATTCGCCAA AAGAGCAATT 
GAAAGTTTAG TTAAAAAGCT GAAGGAGAAA AAAGATGAAT TGGATTCTTT AATAACAGCT 
ATAACTACAA ATGGAGCTCA CCCTAGCAAG TGTGTTACCA TACAGAGAAC ATTGGATGGA 
AGGCTTCAGG TGGCTGGTCG CAAGGGATTT CCTCATGTGA TCTATGCCCG TCTCTGGAGG 
TGGCCTGACC TTCACAAAAA TGAACTAAAA CATGTTAAAT ATTGTCAGTA TGCATTTGAC 
TTAAAATGTG ATAGTGTCTG TGTGAATCCA TATCACTATG AACGAGTTGT GTCACCTGGA 
ATTGATCTCT CAGGATTAAC ACTGCAGAGT AATGCTCCAC CAAGTATGTT GGTGAAGGAT 
GAATATGTTC ATGACTTTGA GGGACAGCCA TCATTATCCA CTGAAGGGCA TTCAATTCAA 
ACCATCCAGC ATCCACCAAG TAATCGTGCA TCGACGGAGA CATACAGCAC CCCAGCTCTC 
TTAGCTCCAT CTGAGTCTAA TGCTACCAGC ACCACCAATT TTCCCAACAT TCCTGTGGCT 
TCCACAAGTC AGCCCGCCAG TATACTGGCA GGCAGCCATA GTGAAGGATT GTTGCAGATA 
GCTTCAGGGC CTCAGCCAGG ACAGCAGCAG AATGGATTTA CTGGTCAGCC AGCTACTTAT 
CATCATAACA GCACTACCAC CTGGACGGGA AGTAGGACTG CACCATACAC ACCTAATTTG 
CCTCACCACC AAAACGGCCA TCTCCAGCAC CACCCGCCTA TGCCGCCCCA TCCCGGACAT 
TACTGGCCAC CAGTTCACAA TGAGCTTGCA TTCCAGCCTC CTATTTCTAA TCATCCTGCT 
CCTGAGTATT GGTGTTCCAT TGCTTACTTC GAGATGGATG TTCAAGTAGG AGAGACATTT 
AAGGTTCCTT CAAGCTGTCC TATTGTTACT GTTGATGGAT ATGTAGACCC TTCTGGAGGA 
GATCGCTTTT GCTTGGGTCA ACTCTCCAAT GTCCACAGGA CAGAAGCCAT TGAGAGAGCA 
AGGTTGCACA TAGGCAAAGG TGTGCAGTTG GAATGTAAAG GTGAAGGTGA TGTTTGGGTC 
AGGTGCCTTA GTGACCACGC AGTCTTTGTA CAGAGCTACT ACTTAGACAG AGAAGCTGGG 
CGTGCACCTG GAGATGCTGT TCATAAGATC TACCCAAGTG CATATATAAA GGTCTTTGAT 
TTGCGTCAGT GTCACCGTCA GATGCAGCAG CAGGCAGCCA CGGCACAAGC TGCAGCAGCT 
GCCCAGGCAG CGGCCGTGGC AGGAAACATC CCCGGCCCAG GGTCAGTAGG TGGAATAGCC 
CCAGCCATCA AGTTAAACTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315595.1
CDS	529..1992
Translation	MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPPSMLVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTTNFPNIPVASTSQPASILAGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAIKLNS

Target ORF information:

RefSeq Version	XM_015460109.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SMAD family member 4 (SMAD4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460109.1

ORF Insert Sequence:

ATGGACAATA TGTCTATTAC AAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTA 
CATAGTTTGA TGTGCCATAG ACAAGGTGGA GAGAGTGAAA CATTCGCCAA AAGAGCAATT 
GAAAGTTTAG TTAAAAAGCT GAAGGAGAAA AAAGATGAAT TGGATTCTTT AATAACAGCT 
ATAACTACAA ATGGAGCTCA CCCTAGCAAG TGTGTTACCA TACAGAGAAC ATTGGATGGA 
AGGCTTCAGG TGGCTGGTCG CAAGGGATTT CCTCATGTGA TCTATGCCCG TCTCTGGAGG 
TGGCCTGACC TTCACAAAAA TGAACTAAAA CATGTTAAAT ATTGTCAGTA TGCATTTGAC 
TTAAAATGTG ATAGTGTCTG TGTGAATCCA TATCACTATG AACGAGTTGT GTCACCTGGA 
ATTGATCTCT CAGGATTAAC ACTGCAGAGT AATGCTCCAC CAAGTATGTT GGTGAAGGAT 
GAATATGTTC ATGACTTTGA GGGACAGCCA TCATTATCCA CTGAAGGGCA TTCAATTCAA 
ACCATCCAGC ATCCACCAAG TAATCGTGCA TCGACGGAGA CATACAGCAC CCCAGCTCTC 
TTAGCTCCAT CTGAGTCTAA TGCTACCAGC ACCACCAATT TTCCCAACAT TCCTGTGGCT 
TCCACAAGTC AGCCCGCCAG TATACTGGCA GGCAGCCATA GTGAAGGATT GTTGCAGATA 
GCTTCAGGGC CTCAGCCAGG ACAGCAGCAG AATGGATTTA CTGGTCAGCC AGCTACTTAT 
CATCATAACA GCACTACCAC CTGGACGGGA AGTAGGACTG CACCATACAC ACCTAATTTG 
CCTCACCACC AAAACGGCCA TCTCCAGCAC CACCCGCCTA TGCCGCCCCA TCCCGGACAT 
TACTGGCCAC CAGTTCACAA TGAGCTTGCA TTCCAGCCTC CTATTTCTAA TCATCCTGCT 
CCTGAGTATT GGTGTTCCAT TGCTTACTTC GAGATGGATG TTCAAGTAGG AGAGACATTT 
AAGGTTCCTT CAAGCTGTCC TATTGTTACT GTTGATGGAT ATGTAGACCC TTCTGGAGGA 
GATCGCTTTT GCTTGGGTCA ACTCTCCAAT GTCCACAGGA CAGAAGCCAT TGAGAGAGCA 
AGGTTGCACA TAGGCAAAGG TGTGCAGTTG GAATGTAAAG GTGAAGGTGA TGTTTGGGTC 
AGGTGCCTTA GTGACCACGC AGTCTTTGTA CAGAGCTACT ACTTAGACAG AGAAGCTGGG 
CGTGCACCTG GAGATGCTGT TCATAAGATC TACCCAAGTG CATATATAAA GGTCTTTGAT 
TTGCGTCAGT GTCACCGTCA GATGCAGCAG CAGGCAGCCA CGGCACAAGC TGCAGCAGCT 
GCCCAGGCAG CGGCCGTGGC AGGAAACATC CCCGGCCCAG GGTCAGTAGG TGGAATAGCC 
CCAGCCATCA AGTTAAACTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evidence supporting a functional requirement of SMAD4 for bovine preimplantation embryonic development: a potential link to embryotrophic actions of follistatin.
Biology of reproduction91(3)62(2014 Sep)
Lee KB,Zhang K,Folger JK,Knott JG,Smith GW

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

ALK5 and Smad4 are involved in TGF-beta1-induced pulmonary endothelial permeability.
FEBS letters579(18)4031-7(2005 Jul)
Birukova AA,Adyshev D,Gorshkov B,Birukov KG,Verin AD

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SMAD4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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