TOGARAM1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010817289.2	XP_010815591.1	protein FAM179B isoform X4
XM_010817290.2	XP_010815592.1	protein FAM179B isoform X5
XM_010817289.3	XP_010815591.2	TOG array regulator of axonemal microtubules protein 1 isoform X4
XM_010824927.2	XP_010823229.1	protein FAM179B isoform X5
XM_010817286.2	XP_010815588.1	protein FAM179B isoform X1
XM_010817287.2	XP_010815589.1	protein FAM179B isoform X2
XM_010817290.3	XP_010815592.2	TOG array regulator of axonemal microtubules protein 1 isoform X6
XM_010824922.2	XP_010823224.1	protein FAM179B isoform X1
XM_010824924.2	XP_010823226.1	protein FAM179B isoform X3
XM_010817288.2	XP_010815590.1	protein FAM179B isoform X3
XM_010824926.2	XP_010823228.1	protein FAM179B isoform X4
XM_010817287.3	XP_010815589.2	TOG array regulator of axonemal microtubules protein 1 isoform X2
XM_010817288.3	XP_010815590.2	TOG array regulator of axonemal microtubules protein 1 isoform X3
XM_010824923.2	XP_010823225.1	protein FAM179B isoform X2
XM_010817286.3	XP_010815588.2	TOG array regulator of axonemal microtubules protein 1 isoform X1
XM_024982094.1	XP_024837862.1	TOG array regulator of axonemal microtubules protein 1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa228500	XM_010817286.3 Latest version!	Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228501	XM_010817287.3 Latest version!	Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228502	XM_010817288.3 Latest version!	Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228503	XM_010817289.3 Latest version!	Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228504	XM_024982094.1 Latest version!	Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228505	XM_010817290.3 Latest version!	Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa54592	XM_010817286.2 Latest version!	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa54592	XM_010824922.2 Latest version!	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa54593	XM_010824923.2 Latest version!	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa54593	XM_010817287.2 Latest version!	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa54594	XM_010824924.2 Latest version!	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa54594	XM_010817288.2 Latest version!	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa54595	XM_010817289.2 Latest version!	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa54595	XM_010824926.2 Latest version!	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa54596	XM_010824927.2 Latest version!	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa54596	XM_010817290.2 Latest version!	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

TOGARAM1 ( XM_010817286.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815588.2 Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa228500
Clone ID Related Accession (Same CDS sequence)	XM_010817286.3
Accession Version	XM_010817286.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TOG array regulator of axonemal microtubules protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817286.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACATG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAATATGT TTCTTTGTTC TAGATGAAGC ATCTCCTGGA 
GCCATTCCAC AGTATAAAGA AAGGATGCCC TCTGTCACTC ATAGTCCAGA AATAATGGAT 
TCATCAGAAC TACGGCCATT TTCCAAACCC GAAGTAGCAC TGACAGAAGC TCTGAGGCTT 
TTAGCTGATG AGGATTGGGA GAAGAAAATT GAGGGACTGA ACTTTATTAG ATGTTTAGCT 
GCTTTTCATT CTGAAATACT GAACACAAAG TTGCATGAAA CAAATTTTGC AGTAGTTCAA 
GAGGTAAAAA ATTTACGCTC TGGAGTTTCT CGTGCTGCTG TGGTCTGTTT AAGTGATCTT 
TTTACTCACC TGAAAAAGAG CATGGATCAA GAACTAGATA ATACCGTAAA AGTTTTATTG 
CAGAAGTCAG GAGAATCAAA TACATTTATA AGAGAAGATG TTGACAAGGC ATTAAGAGCT 
ATGATCACTA ATGTAACTCC CGCACGTGCG GTTGTTTCTC TTATCAACGG AGGACAAAGC 
CACTTACACA TTGCAGTAAG AAGATGTGCT GCCCAGCATT TATCAGATGT GGTAGAATTT 
ATGGAACCAG AGCGTGTTTT ATCTGGAACA AAGGATATGG CTGACCGTAT ATTACCCGCT 
GCTGCTAAAT TTGCTCAGGA CAGCTCACCA GAAACCAGGT ATTATGGCCG AAAGATGCTT 
TTCCTCATGA TGTGTCATCC TAACTTTGAT AAAATGCTTG AAAAGTACGT GCCATCTAAA 
GATTTGCCAT ATATTAAGGA ATCTGTTAAA AGCTTACGGC AAAAGGGTTT GGGAGAGATA 
CCGCTAGATA CTCCTTCAGC AAAAGGAAGA CGATCTCATA CCGGCAGTGT TGGAAACACA 
AGGTCGTCAT CTGTTTCTAG GGATGCTTTC AATTCAGCTG AAAGAGAGGT AACTGAAATT 
CGTGAAATCA CCAGAAAATC AGTTCCCCGA AATTCCTTAG AAAGTGCTGA GTACATTAAA 
GTCATAACAG GTTTATTAAA TGCAAAAGAT TTTCGTGATC GTATTAATGG GATTAAGCAG 
CTTTTATCAG ATACAGAAAA CAATCAAGAG CTTGTTGTTG GAAACATTGT GAAGATCTTT 
GATGCTTTTA AATCTCGGCT TCATGATTCC AATAGTAAAG TAAACCTGGT GGCTCTAGAA 
ACAATGCACA AAATGATTCC TTTGCTCAGA GACAACTTAT CTCCTATAAT CAACATGCTA 
ATCCCAGCAA TAGTAGATAA CAATCTGAAT TCCAAGAATC CAGGCATCTA TGCTGCTGCC 
ACCAATGTTG TTCAGGCACT GAGTCAGCAC GTAGATAATT ACTTACTTCT ACAGCCATTT 
TGCACAAAAG CCCAGTTTTT AAATGGAAAG GCAAAACAGG ATATGACTGA AAAGCTTGCT 
GATATTGTTA TGGAACTTTA TCAAAGGAAG CCCCATGCAA CAGAACAGAA AGTGTTGGTT 
GTTTTATGGC ATCTCTTAGG GAACATGACA AATAGTGGCT CTTTGCCTGG AGCTGGAGGA 
AATATACGAA CAGCCACAGC TAAATTATCA AAAGCACTTT TTACACAGAT GGGTCAGAAT 
CTGTTAAATC AGGCTGCATC TCAACCATCA CATATAAAAA AGAGTTTAGA AGAATTGCTT 
GATATGACAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815588.2
CDS	198..5672
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAGTSGTHQTNLSGKCGQLGFSQICGKTGSVDSDLQYLGTTNSHQDKVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHMSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTGTTTSSVSIIGHRMNYGFGCGDFEEDRSHDISPSALKIQNKEQPRHYKHTKASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQICFFVLDEASPGAIPQYKERMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIFDAFKSRLHDSNSKVNLVALETMHKMIPLLRDNLSPIINMLIPAIVDNNLNSKNPGIYAAATNVVQALSQHVDNYLLLQPFCTKAQFLNGKAKQDMTEKLADIVMELYQRKPHATEQKVLVVLWHLLGNMTNSGSLPGAGGNIRTATAKLSKALFTQMGQNLLNQAASQPSHIKKSLEELLDMTI

Target ORF information:

RefSeq Version	XM_010817286.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817286.3

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACATG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAATATGT TTCTTTGTTC TAGATGAAGC ATCTCCTGGA 
GCCATTCCAC AGTATAAAGA AAGGATGCCC TCTGTCACTC ATAGTCCAGA AATAATGGAT 
TCATCAGAAC TACGGCCATT TTCCAAACCC GAAGTAGCAC TGACAGAAGC TCTGAGGCTT 
TTAGCTGATG AGGATTGGGA GAAGAAAATT GAGGGACTGA ACTTTATTAG ATGTTTAGCT 
GCTTTTCATT CTGAAATACT GAACACAAAG TTGCATGAAA CAAATTTTGC AGTAGTTCAA 
GAGGTAAAAA ATTTACGCTC TGGAGTTTCT CGTGCTGCTG TGGTCTGTTT AAGTGATCTT 
TTTACTCACC TGAAAAAGAG CATGGATCAA GAACTAGATA ATACCGTAAA AGTTTTATTG 
CAGAAGTCAG GAGAATCAAA TACATTTATA AGAGAAGATG TTGACAAGGC ATTAAGAGCT 
ATGATCACTA ATGTAACTCC CGCACGTGCG GTTGTTTCTC TTATCAACGG AGGACAAAGC 
CACTTACACA TTGCAGTAAG AAGATGTGCT GCCCAGCATT TATCAGATGT GGTAGAATTT 
ATGGAACCAG AGCGTGTTTT ATCTGGAACA AAGGATATGG CTGACCGTAT ATTACCCGCT 
GCTGCTAAAT TTGCTCAGGA CAGCTCACCA GAAACCAGGT ATTATGGCCG AAAGATGCTT 
TTCCTCATGA TGTGTCATCC TAACTTTGAT AAAATGCTTG AAAAGTACGT GCCATCTAAA 
GATTTGCCAT ATATTAAGGA ATCTGTTAAA AGCTTACGGC AAAAGGGTTT GGGAGAGATA 
CCGCTAGATA CTCCTTCAGC AAAAGGAAGA CGATCTCATA CCGGCAGTGT TGGAAACACA 
AGGTCGTCAT CTGTTTCTAG GGATGCTTTC AATTCAGCTG AAAGAGAGGT AACTGAAATT 
CGTGAAATCA CCAGAAAATC AGTTCCCCGA AATTCCTTAG AAAGTGCTGA GTACATTAAA 
GTCATAACAG GTTTATTAAA TGCAAAAGAT TTTCGTGATC GTATTAATGG GATTAAGCAG 
CTTTTATCAG ATACAGAAAA CAATCAAGAG CTTGTTGTTG GAAACATTGT GAAGATCTTT 
GATGCTTTTA AATCTCGGCT TCATGATTCC AATAGTAAAG TAAACCTGGT GGCTCTAGAA 
ACAATGCACA AAATGATTCC TTTGCTCAGA GACAACTTAT CTCCTATAAT CAACATGCTA 
ATCCCAGCAA TAGTAGATAA CAATCTGAAT TCCAAGAATC CAGGCATCTA TGCTGCTGCC 
ACCAATGTTG TTCAGGCACT GAGTCAGCAC GTAGATAATT ACTTACTTCT ACAGCCATTT 
TGCACAAAAG CCCAGTTTTT AAATGGAAAG GCAAAACAGG ATATGACTGA AAAGCTTGCT 
GATATTGTTA TGGAACTTTA TCAAAGGAAG CCCCATGCAA CAGAACAGAA AGTGTTGGTT 
GTTTTATGGC ATCTCTTAGG GAACATGACA AATAGTGGCT CTTTGCCTGG AGCTGGAGGA 
AATATACGAA CAGCCACAGC TAAATTATCA AAAGCACTTT TTACACAGAT GGGTCAGAAT 
CTGTTAAATC AGGCTGCATC TCAACCATCA CATATAAAAA AGAGTTTAGA AGAATTGCTT 
GATATGACAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_010817287.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815589.2 Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X2, mRNA.

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CloneID	OBa228501
Clone ID Related Accession (Same CDS sequence)	XM_010817287.3
Accession Version	XM_010817287.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TOG array regulator of axonemal microtubules protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817287.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACATG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAAATGAA GCATCTCCTG GAGCCATTCC ACAGTATAAA 
GAAAGGATGC CCTCTGTCAC TCATAGTCCA GAAATAATGG ATTCATCAGA ACTACGGCCA 
TTTTCCAAAC CCGAAGTAGC ACTGACAGAA GCTCTGAGGC TTTTAGCTGA TGAGGATTGG 
GAGAAGAAAA TTGAGGGACT GAACTTTATT AGATGTTTAG CTGCTTTTCA TTCTGAAATA 
CTGAACACAA AGTTGCATGA AACAAATTTT GCAGTAGTTC AAGAGGTAAA AAATTTACGC 
TCTGGAGTTT CTCGTGCTGC TGTGGTCTGT TTAAGTGATC TTTTTACTCA CCTGAAAAAG 
AGCATGGATC AAGAACTAGA TAATACCGTA AAAGTTTTAT TGCAGAAGTC AGGAGAATCA 
AATACATTTA TAAGAGAAGA TGTTGACAAG GCATTAAGAG CTATGATCAC TAATGTAACT 
CCCGCACGTG CGGTTGTTTC TCTTATCAAC GGAGGACAAA GCCACTTACA CATTGCAGTA 
AGAAGATGTG CTGCCCAGCA TTTATCAGAT GTGGTAGAAT TTATGGAACC AGAGCGTGTT 
TTATCTGGAA CAAAGGATAT GGCTGACCGT ATATTACCCG CTGCTGCTAA ATTTGCTCAG 
GACAGCTCAC CAGAAACCAG GTATTATGGC CGAAAGATGC TTTTCCTCAT GATGTGTCAT 
CCTAACTTTG ATAAAATGCT TGAAAAGTAC GTGCCATCTA AAGATTTGCC ATATATTAAG 
GAATCTGTTA AAAGCTTACG GCAAAAGGGT TTGGGAGAGA TACCGCTAGA TACTCCTTCA 
GCAAAAGGAA GACGATCTCA TACCGGCAGT GTTGGAAACA CAAGGTCGTC ATCTGTTTCT 
AGGGATGCTT TCAATTCAGC TGAAAGAGAG GTAACTGAAA TTCGTGAAAT CACCAGAAAA 
TCAGTTCCCC GAAATTCCTT AGAAAGTGCT GAGTACATTA AAGTCATAAC AGGTTTATTA 
AATGCAAAAG ATTTTCGTGA TCGTATTAAT GGGATTAAGC AGCTTTTATC AGATACAGAA 
AACAATCAAG AGCTTGTTGT TGGAAACATT GTGAAGATCT TTGATGCTTT TAAATCTCGG 
CTTCATGATT CCAATAGTAA AGTAAACCTG GTGGCTCTAG AAACAATGCA CAAAATGATT 
CCTTTGCTCA GAGACAACTT ATCTCCTATA ATCAACATGC TAATCCCAGC AATAGTAGAT 
AACAATCTGA ATTCCAAGAA TCCAGGCATC TATGCTGCTG CCACCAATGT TGTTCAGGCA 
CTGAGTCAGC ACGTAGATAA TTACTTACTT CTACAGCCAT TTTGCACAAA AGCCCAGTTT 
TTAAATGGAA AGGCAAAACA GGATATGACT GAAAAGCTTG CTGATATTGT TATGGAACTT 
TATCAAAGGA AGCCCCATGC AACAGAACAG AAAGTGTTGG TTGTTTTATG GCATCTCTTA 
GGGAACATGA CAAATAGTGG CTCTTTGCCT GGAGCTGGAG GAAATATACG AACAGCCACA 
GCTAAATTAT CAAAAGCACT TTTTACACAG ATGGGTCAGA ATCTGTTAAA TCAGGCTGCA 
TCTCAACCAT CACATATAAA AAAGAGTTTA GAAGAATTGC TTGATATGAC AATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815589.2
CDS	193..5649
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAGTSGTHQTNLSGKCGQLGFSQICGKTGSVDSDLQYLGTTNSHQDKVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHMSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTGTTTSSVSIIGHRMNYGFGCGDFEEDRSHDISPSALKIQNKEQPRHYKHTKASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQNEASPGAIPQYKERMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIFDAFKSRLHDSNSKVNLVALETMHKMIPLLRDNLSPIINMLIPAIVDNNLNSKNPGIYAAATNVVQALSQHVDNYLLLQPFCTKAQFLNGKAKQDMTEKLADIVMELYQRKPHATEQKVLVVLWHLLGNMTNSGSLPGAGGNIRTATAKLSKALFTQMGQNLLNQAASQPSHIKKSLEELLDMTI

Target ORF information:

RefSeq Version	XM_010817287.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817287.3

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACATG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAAATGAA GCATCTCCTG GAGCCATTCC ACAGTATAAA 
GAAAGGATGC CCTCTGTCAC TCATAGTCCA GAAATAATGG ATTCATCAGA ACTACGGCCA 
TTTTCCAAAC CCGAAGTAGC ACTGACAGAA GCTCTGAGGC TTTTAGCTGA TGAGGATTGG 
GAGAAGAAAA TTGAGGGACT GAACTTTATT AGATGTTTAG CTGCTTTTCA TTCTGAAATA 
CTGAACACAA AGTTGCATGA AACAAATTTT GCAGTAGTTC AAGAGGTAAA AAATTTACGC 
TCTGGAGTTT CTCGTGCTGC TGTGGTCTGT TTAAGTGATC TTTTTACTCA CCTGAAAAAG 
AGCATGGATC AAGAACTAGA TAATACCGTA AAAGTTTTAT TGCAGAAGTC AGGAGAATCA 
AATACATTTA TAAGAGAAGA TGTTGACAAG GCATTAAGAG CTATGATCAC TAATGTAACT 
CCCGCACGTG CGGTTGTTTC TCTTATCAAC GGAGGACAAA GCCACTTACA CATTGCAGTA 
AGAAGATGTG CTGCCCAGCA TTTATCAGAT GTGGTAGAAT TTATGGAACC AGAGCGTGTT 
TTATCTGGAA CAAAGGATAT GGCTGACCGT ATATTACCCG CTGCTGCTAA ATTTGCTCAG 
GACAGCTCAC CAGAAACCAG GTATTATGGC CGAAAGATGC TTTTCCTCAT GATGTGTCAT 
CCTAACTTTG ATAAAATGCT TGAAAAGTAC GTGCCATCTA AAGATTTGCC ATATATTAAG 
GAATCTGTTA AAAGCTTACG GCAAAAGGGT TTGGGAGAGA TACCGCTAGA TACTCCTTCA 
GCAAAAGGAA GACGATCTCA TACCGGCAGT GTTGGAAACA CAAGGTCGTC ATCTGTTTCT 
AGGGATGCTT TCAATTCAGC TGAAAGAGAG GTAACTGAAA TTCGTGAAAT CACCAGAAAA 
TCAGTTCCCC GAAATTCCTT AGAAAGTGCT GAGTACATTA AAGTCATAAC AGGTTTATTA 
AATGCAAAAG ATTTTCGTGA TCGTATTAAT GGGATTAAGC AGCTTTTATC AGATACAGAA 
AACAATCAAG AGCTTGTTGT TGGAAACATT GTGAAGATCT TTGATGCTTT TAAATCTCGG 
CTTCATGATT CCAATAGTAA AGTAAACCTG GTGGCTCTAG AAACAATGCA CAAAATGATT 
CCTTTGCTCA GAGACAACTT ATCTCCTATA ATCAACATGC TAATCCCAGC AATAGTAGAT 
AACAATCTGA ATTCCAAGAA TCCAGGCATC TATGCTGCTG CCACCAATGT TGTTCAGGCA 
CTGAGTCAGC ACGTAGATAA TTACTTACTT CTACAGCCAT TTTGCACAAA AGCCCAGTTT 
TTAAATGGAA AGGCAAAACA GGATATGACT GAAAAGCTTG CTGATATTGT TATGGAACTT 
TATCAAAGGA AGCCCCATGC AACAGAACAG AAAGTGTTGG TTGTTTTATG GCATCTCTTA 
GGGAACATGA CAAATAGTGG CTCTTTGCCT GGAGCTGGAG GAAATATACG AACAGCCACA 
GCTAAATTAT CAAAAGCACT TTTTACACAG ATGGGTCAGA ATCTGTTAAA TCAGGCTGCA 
TCTCAACCAT CACATATAAA AAAGAGTTTA GAAGAATTGC TTGATATGAC AATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_010817288.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815590.2 Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X3, mRNA.

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CloneID	OBa228502
Clone ID Related Accession (Same CDS sequence)	XM_010817288.3
Accession Version	XM_010817288.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TOG array regulator of axonemal microtubules protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817288.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGTTTGTGC TAGCCTATGT 
TTTGGTAGTA AGACACAGCA GACATTTGGT AGTCAAACAG AGCGTTCGTC ATACTCTGGT 
CCAAATTCAA GTTCAGGAGC TGTTCTTCTT CCATCCTATC CTCTCTCGTC ACCTCGAACT 
AGTCCAAAGC ACATGTCTCT TACTGTGTCT CCAAAGAAGT CTCAAGATAA TTCTGTTAAT 
TTCTCAAGTT CCTGGCCTCT TAAAAGCTTT GAAGGGCTTC CAAAGCCAAG TCCACAGAAA 
AAGCTTGTCA GCCAAAAATC ATCTGATCCT ACAGGAGAAA ATTCTCAAGA GAAACCTCCT 
CAAGTTCAAC TTACTCCTGC TTTGGTGAGA TCACCGTCTT CCCGACGAGG CTTAAATGGG 
ACCAGACCTG TACCTCCCAT ACCACGGGGA CTCAGCCTTC TGCCTGATAA AGCTGGTCTA 
AGCACAGTGG GACCCAAAAA GAAAGAGCCT GATGACATTT GGAAGAGTGA AAAAGATAAT 
CTTACAGTTG ATCTTTCAGA ATTAAATATC AGGGATAAAG ATTTGGATCA AGAAGAGATG 
CAGAGCTCTC TTAGGTCCCT TCGTAATAGC GCAGCTAAGA AAAGAGCAAA ACTGAGTGGC 
AGTACTTCTG ATCTTGAAAG CCCAGATTCC GCCATGAAGC TTGACTTGAC TGTGGATTCC 
CCATCTCGAT CTTCCTCTCC AAATACCAGT TCCTACAGTG AAAGTGGAGT TTACAGCCAA 
GAATCCTTGA CTTCTTCTCT GTCGACAACT CCCCAGGGGA AGAGAATAAT GTCAGACATA 
TTTCCAACTT TTGGATCAAA ACCTTGTCCA ACAAGACTTT CTTCAGCGAA GAATAAGAAC 
TCTCAGGTAG CTGAACACAG CCCCAGTACA GGGACTACAA CATCCAGTGT AAGCATAATT 
GGTCATCGTA TGAACTATGG GTTTGGATGT GGTGACTTTG AAGAGGATAG GTCACATGAC 
ATTTCACCTA GTGCTTTAAA AATACAAAAT AAGGAACAAC CAAGACATTA TAAGCATACG 
AAAGCATCTA CAGGGTCATC ATCAAATTTC CACCAAATTT CCAGTTTTGA CTTCATGTCT 
ACAAATACCT TATCAGAAGA CTCAGTAGTA GTTGTTGGAA AAGGGGTATT TGGAAATCCA 
ATTCCAGCAC CAGCAACCTG CAGTCAATCA GTGATAACTT CTGTGGAAAA TGGGGTTTCG 
TTTTCAGTTA AAAATACTGA ACCACCATCA GGCATTTATG GAAGAGCAGT TCAGCAGAGT 
ATTCCATCAT ATGTTGATTC TGAGAATGAA AAAGATATTA AAGTTTCTAT TTCAAAATCT 
ACTTATGACA AGATGAGACA AAAGAGAAAA GAGGAAAAAG AACTTTTTGA CATTAAAGAT 
TCTGAAAGGA AGGAACAGAA TCCCTGGGAA CGAATGAAAT ATACAGGAAA TGAAAAAATG 
GCACCTCAAA TATGTTTCTT TGTTCTAGAT GAAGCATCTC CTGGAGCCAT TCCACAGTAT 
AAAGAAAGGA TGCCCTCTGT CACTCATAGT CCAGAAATAA TGGATTCATC AGAACTACGG 
CCATTTTCCA AACCCGAAGT AGCACTGACA GAAGCTCTGA GGCTTTTAGC TGATGAGGAT 
TGGGAGAAGA AAATTGAGGG ACTGAACTTT ATTAGATGTT TAGCTGCTTT TCATTCTGAA 
ATACTGAACA CAAAGTTGCA TGAAACAAAT TTTGCAGTAG TTCAAGAGGT AAAAAATTTA 
CGCTCTGGAG TTTCTCGTGC TGCTGTGGTC TGTTTAAGTG ATCTTTTTAC TCACCTGAAA 
AAGAGCATGG ATCAAGAACT AGATAATACC GTAAAAGTTT TATTGCAGAA GTCAGGAGAA 
TCAAATACAT TTATAAGAGA AGATGTTGAC AAGGCATTAA GAGCTATGAT CACTAATGTA 
ACTCCCGCAC GTGCGGTTGT TTCTCTTATC AACGGAGGAC AAAGCCACTT ACACATTGCA 
GTAAGAAGAT GTGCTGCCCA GCATTTATCA GATGTGGTAG AATTTATGGA ACCAGAGCGT 
GTTTTATCTG GAACAAAGGA TATGGCTGAC CGTATATTAC CCGCTGCTGC TAAATTTGCT 
CAGGACAGCT CACCAGAAAC CAGGTATTAT GGCCGAAAGA TGCTTTTCCT CATGATGTGT 
CATCCTAACT TTGATAAAAT GCTTGAAAAG TACGTGCCAT CTAAAGATTT GCCATATATT 
AAGGAATCTG TTAAAAGCTT ACGGCAAAAG GGTTTGGGAG AGATACCGCT AGATACTCCT 
TCAGCAAAAG GAAGACGATC TCATACCGGC AGTGTTGGAA ACACAAGGTC GTCATCTGTT 
TCTAGGGATG CTTTCAATTC AGCTGAAAGA GAGGTAACTG AAATTCGTGA AATCACCAGA 
AAATCAGTTC CCCGAAATTC CTTAGAAAGT GCTGAGTACA TTAAAGTCAT AACAGGTTTA 
TTAAATGCAA AAGATTTTCG TGATCGTATT AATGGGATTA AGCAGCTTTT ATCAGATACA 
GAAAACAATC AAGAGCTTGT TGTTGGAAAC ATTGTGAAGA TCTTTGATGC TTTTAAATCT 
CGGCTTCATG ATTCCAATAG TAAAGTAAAC CTGGTGGCTC TAGAAACAAT GCACAAAATG 
ATTCCTTTGC TCAGAGACAA CTTATCTCCT ATAATCAACA TGCTAATCCC AGCAATAGTA 
GATAACAATC TGAATTCCAA GAATCCAGGC ATCTATGCTG CTGCCACCAA TGTTGTTCAG 
GCACTGAGTC AGCACGTAGA TAATTACTTA CTTCTACAGC CATTTTGCAC AAAAGCCCAG 
TTTTTAAATG GAAAGGCAAA ACAGGATATG ACTGAAAAGC TTGCTGATAT TGTTATGGAA 
CTTTATCAAA GGAAGCCCCA TGCAACAGAA CAGAAAGTGT TGGTTGTTTT ATGGCATCTC 
TTAGGGAACA TGACAAATAG TGGCTCTTTG CCTGGAGCTG GAGGAAATAT ACGAACAGCC 
ACAGCTAAAT TATCAAAAGC ACTTTTTACA CAGATGGGTC AGAATCTGTT AAATCAGGCT 
GCATCTCAAC CATCACATAT AAAAAAGAGT TTAGAAGAAT TGCTTGATAT GACAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815590.2
CDS	199..5538
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHMSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTGTTTSSVSIIGHRMNYGFGCGDFEEDRSHDISPSALKIQNKEQPRHYKHTKASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQICFFVLDEASPGAIPQYKERMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIFDAFKSRLHDSNSKVNLVALETMHKMIPLLRDNLSPIINMLIPAIVDNNLNSKNPGIYAAATNVVQALSQHVDNYLLLQPFCTKAQFLNGKAKQDMTEKLADIVMELYQRKPHATEQKVLVVLWHLLGNMTNSGSLPGAGGNIRTATAKLSKALFTQMGQNLLNQAASQPSHIKKSLEELLDMTI

Target ORF information:

RefSeq Version	XM_010817288.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817288.3

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGTTTGTGC TAGCCTATGT 
TTTGGTAGTA AGACACAGCA GACATTTGGT AGTCAAACAG AGCGTTCGTC ATACTCTGGT 
CCAAATTCAA GTTCAGGAGC TGTTCTTCTT CCATCCTATC CTCTCTCGTC ACCTCGAACT 
AGTCCAAAGC ACATGTCTCT TACTGTGTCT CCAAAGAAGT CTCAAGATAA TTCTGTTAAT 
TTCTCAAGTT CCTGGCCTCT TAAAAGCTTT GAAGGGCTTC CAAAGCCAAG TCCACAGAAA 
AAGCTTGTCA GCCAAAAATC ATCTGATCCT ACAGGAGAAA ATTCTCAAGA GAAACCTCCT 
CAAGTTCAAC TTACTCCTGC TTTGGTGAGA TCACCGTCTT CCCGACGAGG CTTAAATGGG 
ACCAGACCTG TACCTCCCAT ACCACGGGGA CTCAGCCTTC TGCCTGATAA AGCTGGTCTA 
AGCACAGTGG GACCCAAAAA GAAAGAGCCT GATGACATTT GGAAGAGTGA AAAAGATAAT 
CTTACAGTTG ATCTTTCAGA ATTAAATATC AGGGATAAAG ATTTGGATCA AGAAGAGATG 
CAGAGCTCTC TTAGGTCCCT TCGTAATAGC GCAGCTAAGA AAAGAGCAAA ACTGAGTGGC 
AGTACTTCTG ATCTTGAAAG CCCAGATTCC GCCATGAAGC TTGACTTGAC TGTGGATTCC 
CCATCTCGAT CTTCCTCTCC AAATACCAGT TCCTACAGTG AAAGTGGAGT TTACAGCCAA 
GAATCCTTGA CTTCTTCTCT GTCGACAACT CCCCAGGGGA AGAGAATAAT GTCAGACATA 
TTTCCAACTT TTGGATCAAA ACCTTGTCCA ACAAGACTTT CTTCAGCGAA GAATAAGAAC 
TCTCAGGTAG CTGAACACAG CCCCAGTACA GGGACTACAA CATCCAGTGT AAGCATAATT 
GGTCATCGTA TGAACTATGG GTTTGGATGT GGTGACTTTG AAGAGGATAG GTCACATGAC 
ATTTCACCTA GTGCTTTAAA AATACAAAAT AAGGAACAAC CAAGACATTA TAAGCATACG 
AAAGCATCTA CAGGGTCATC ATCAAATTTC CACCAAATTT CCAGTTTTGA CTTCATGTCT 
ACAAATACCT TATCAGAAGA CTCAGTAGTA GTTGTTGGAA AAGGGGTATT TGGAAATCCA 
ATTCCAGCAC CAGCAACCTG CAGTCAATCA GTGATAACTT CTGTGGAAAA TGGGGTTTCG 
TTTTCAGTTA AAAATACTGA ACCACCATCA GGCATTTATG GAAGAGCAGT TCAGCAGAGT 
ATTCCATCAT ATGTTGATTC TGAGAATGAA AAAGATATTA AAGTTTCTAT TTCAAAATCT 
ACTTATGACA AGATGAGACA AAAGAGAAAA GAGGAAAAAG AACTTTTTGA CATTAAAGAT 
TCTGAAAGGA AGGAACAGAA TCCCTGGGAA CGAATGAAAT ATACAGGAAA TGAAAAAATG 
GCACCTCAAA TATGTTTCTT TGTTCTAGAT GAAGCATCTC CTGGAGCCAT TCCACAGTAT 
AAAGAAAGGA TGCCCTCTGT CACTCATAGT CCAGAAATAA TGGATTCATC AGAACTACGG 
CCATTTTCCA AACCCGAAGT AGCACTGACA GAAGCTCTGA GGCTTTTAGC TGATGAGGAT 
TGGGAGAAGA AAATTGAGGG ACTGAACTTT ATTAGATGTT TAGCTGCTTT TCATTCTGAA 
ATACTGAACA CAAAGTTGCA TGAAACAAAT TTTGCAGTAG TTCAAGAGGT AAAAAATTTA 
CGCTCTGGAG TTTCTCGTGC TGCTGTGGTC TGTTTAAGTG ATCTTTTTAC TCACCTGAAA 
AAGAGCATGG ATCAAGAACT AGATAATACC GTAAAAGTTT TATTGCAGAA GTCAGGAGAA 
TCAAATACAT TTATAAGAGA AGATGTTGAC AAGGCATTAA GAGCTATGAT CACTAATGTA 
ACTCCCGCAC GTGCGGTTGT TTCTCTTATC AACGGAGGAC AAAGCCACTT ACACATTGCA 
GTAAGAAGAT GTGCTGCCCA GCATTTATCA GATGTGGTAG AATTTATGGA ACCAGAGCGT 
GTTTTATCTG GAACAAAGGA TATGGCTGAC CGTATATTAC CCGCTGCTGC TAAATTTGCT 
CAGGACAGCT CACCAGAAAC CAGGTATTAT GGCCGAAAGA TGCTTTTCCT CATGATGTGT 
CATCCTAACT TTGATAAAAT GCTTGAAAAG TACGTGCCAT CTAAAGATTT GCCATATATT 
AAGGAATCTG TTAAAAGCTT ACGGCAAAAG GGTTTGGGAG AGATACCGCT AGATACTCCT 
TCAGCAAAAG GAAGACGATC TCATACCGGC AGTGTTGGAA ACACAAGGTC GTCATCTGTT 
TCTAGGGATG CTTTCAATTC AGCTGAAAGA GAGGTAACTG AAATTCGTGA AATCACCAGA 
AAATCAGTTC CCCGAAATTC CTTAGAAAGT GCTGAGTACA TTAAAGTCAT AACAGGTTTA 
TTAAATGCAA AAGATTTTCG TGATCGTATT AATGGGATTA AGCAGCTTTT ATCAGATACA 
GAAAACAATC AAGAGCTTGT TGTTGGAAAC ATTGTGAAGA TCTTTGATGC TTTTAAATCT 
CGGCTTCATG ATTCCAATAG TAAAGTAAAC CTGGTGGCTC TAGAAACAAT GCACAAAATG 
ATTCCTTTGC TCAGAGACAA CTTATCTCCT ATAATCAACA TGCTAATCCC AGCAATAGTA 
GATAACAATC TGAATTCCAA GAATCCAGGC ATCTATGCTG CTGCCACCAA TGTTGTTCAG 
GCACTGAGTC AGCACGTAGA TAATTACTTA CTTCTACAGC CATTTTGCAC AAAAGCCCAG 
TTTTTAAATG GAAAGGCAAA ACAGGATATG ACTGAAAAGC TTGCTGATAT TGTTATGGAA 
CTTTATCAAA GGAAGCCCCA TGCAACAGAA CAGAAAGTGT TGGTTGTTTT ATGGCATCTC 
TTAGGGAACA TGACAAATAG TGGCTCTTTG CCTGGAGCTG GAGGAAATAT ACGAACAGCC 
ACAGCTAAAT TATCAAAAGC ACTTTTTACA CAGATGGGTC AGAATCTGTT AAATCAGGCT 
GCATCTCAAC CATCACATAT AAAAAAGAGT TTAGAAGAAT TGCTTGATAT GACAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_010817289.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815591.2 Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa228503
Clone ID Related Accession (Same CDS sequence)	XM_010817289.3
Accession Version	XM_010817289.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TOG array regulator of axonemal microtubules protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817289.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACATG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGCATC TACAGGGTCA 
TCATCAAATT TCCACCAAAT TTCCAGTTTT GACTTCATGT CTACAAATAC CTTATCAGAA 
GACTCAGTAG TAGTTGTTGG AAAAGGGGTA TTTGGAAATC CAATTCCAGC ACCAGCAACC 
TGCAGTCAAT CAGTGATAAC TTCTGTGGAA AATGGGGTTT CGTTTTCAGT TAAAAATACT 
GAACCACCAT CAGGCATTTA TGGAAGAGCA GTTCAGCAGA GTATTCCATC ATATGTTGAT 
TCTGAGAATG AAAAAGATAT TAAAGTTTCT ATTTCAAAAT CTACTTATGA CAAGATGAGA 
CAAAAGAGAA AAGAGGAAAA AGAACTTTTT GACATTAAAG ATTCTGAAAG GAAGGAACAG 
AATCCCTGGG AACGAATGAA ATATACAGGA AATGAAAAAA TGGCACCTCA AATATGTTTC 
TTTGTTCTAG ATGAAGCATC TCCTGGAGCC ATTCCACAGT ATAAAGAAAG GATGCCCTCT 
GTCACTCATA GTCCAGAAAT AATGGATTCA TCAGAACTAC GGCCATTTTC CAAACCCGAA 
GTAGCACTGA CAGAAGCTCT GAGGCTTTTA GCTGATGAGG ATTGGGAGAA GAAAATTGAG 
GGACTGAACT TTATTAGATG TTTAGCTGCT TTTCATTCTG AAATACTGAA CACAAAGTTG 
CATGAAACAA ATTTTGCAGT AGTTCAAGAG GTAAAAAATT TACGCTCTGG AGTTTCTCGT 
GCTGCTGTGG TCTGTTTAAG TGATCTTTTT ACTCACCTGA AAAAGAGCAT GGATCAAGAA 
CTAGATAATA CCGTAAAAGT TTTATTGCAG AAGTCAGGAG AATCAAATAC ATTTATAAGA 
GAAGATGTTG ACAAGGCATT AAGAGCTATG ATCACTAATG TAACTCCCGC ACGTGCGGTT 
GTTTCTCTTA TCAACGGAGG ACAAAGCCAC TTACACATTG CAGTAAGAAG ATGTGCTGCC 
CAGCATTTAT CAGATGTGGT AGAATTTATG GAACCAGAGC GTGTTTTATC TGGAACAAAG 
GATATGGCTG ACCGTATATT ACCCGCTGCT GCTAAATTTG CTCAGGACAG CTCACCAGAA 
ACCAGGTATT ATGGCCGAAA GATGCTTTTC CTCATGATGT GTCATCCTAA CTTTGATAAA 
ATGCTTGAAA AGTACGTGCC ATCTAAAGAT TTGCCATATA TTAAGGAATC TGTTAAAAGC 
TTACGGCAAA AGGGTTTGGG AGAGATACCG CTAGATACTC CTTCAGCAAA AGGAAGACGA 
TCTCATACCG GCAGTGTTGG AAACACAAGG TCGTCATCTG TTTCTAGGGA TGCTTTCAAT 
TCAGCTGAAA GAGAGGTAAC TGAAATTCGT GAAATCACCA GAAAATCAGT TCCCCGAAAT 
TCCTTAGAAA GTGCTGAGTA CATTAAAGTC ATAACAGGTT TATTAAATGC AAAAGATTTT 
CGTGATCGTA TTAATGGGAT TAAGCAGCTT TTATCAGATA CAGAAAACAA TCAAGAGCTT 
GTTGTTGGAA ACATTGTGAA GATCTTTGAT GCTTTTAAAT CTCGGCTTCA TGATTCCAAT 
AGTAAAGTAA ACCTGGTGGC TCTAGAAACA ATGCACAAAA TGATTCCTTT GCTCAGAGAC 
AACTTATCTC CTATAATCAA CATGCTAATC CCAGCAATAG TAGATAACAA TCTGAATTCC 
AAGAATCCAG GCATCTATGC TGCTGCCACC AATGTTGTTC AGGCACTGAG TCAGCACGTA 
GATAATTACT TACTTCTACA GCCATTTTGC ACAAAAGCCC AGTTTTTAAA TGGAAAGGCA 
AAACAGGATA TGACTGAAAA GCTTGCTGAT ATTGTTATGG AACTTTATCA AAGGAAGCCC 
CATGCAACAG AACAGAAAGT GTTGGTTGTT TTATGGCATC TCTTAGGGAA CATGACAAAT 
AGTGGCTCTT TGCCTGGAGC TGGAGGAAAT ATACGAACAG CCACAGCTAA ATTATCAAAA 
GCACTTTTTA CACAGATGGG TCAGAATCTG TTAAATCAGG CTGCATCTCA ACCATCACAT 
ATAAAAAAGA GTTTAGAAGA ATTGCTTGAT ATGACAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815591.2
CDS	198..5519
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAGTSGTHQTNLSGKCGQLGFSQICGKTGSVDSDLQYLGTTNSHQDKVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHMSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQICFFVLDEASPGAIPQYKERMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIFDAFKSRLHDSNSKVNLVALETMHKMIPLLRDNLSPIINMLIPAIVDNNLNSKNPGIYAAATNVVQALSQHVDNYLLLQPFCTKAQFLNGKAKQDMTEKLADIVMELYQRKPHATEQKVLVVLWHLLGNMTNSGSLPGAGGNIRTATAKLSKALFTQMGQNLLNQAASQPSHIKKSLEELLDMTI

Target ORF information:

RefSeq Version	XM_010817289.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817289.3

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACATG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGCATC TACAGGGTCA 
TCATCAAATT TCCACCAAAT TTCCAGTTTT GACTTCATGT CTACAAATAC CTTATCAGAA 
GACTCAGTAG TAGTTGTTGG AAAAGGGGTA TTTGGAAATC CAATTCCAGC ACCAGCAACC 
TGCAGTCAAT CAGTGATAAC TTCTGTGGAA AATGGGGTTT CGTTTTCAGT TAAAAATACT 
GAACCACCAT CAGGCATTTA TGGAAGAGCA GTTCAGCAGA GTATTCCATC ATATGTTGAT 
TCTGAGAATG AAAAAGATAT TAAAGTTTCT ATTTCAAAAT CTACTTATGA CAAGATGAGA 
CAAAAGAGAA AAGAGGAAAA AGAACTTTTT GACATTAAAG ATTCTGAAAG GAAGGAACAG 
AATCCCTGGG AACGAATGAA ATATACAGGA AATGAAAAAA TGGCACCTCA AATATGTTTC 
TTTGTTCTAG ATGAAGCATC TCCTGGAGCC ATTCCACAGT ATAAAGAAAG GATGCCCTCT 
GTCACTCATA GTCCAGAAAT AATGGATTCA TCAGAACTAC GGCCATTTTC CAAACCCGAA 
GTAGCACTGA CAGAAGCTCT GAGGCTTTTA GCTGATGAGG ATTGGGAGAA GAAAATTGAG 
GGACTGAACT TTATTAGATG TTTAGCTGCT TTTCATTCTG AAATACTGAA CACAAAGTTG 
CATGAAACAA ATTTTGCAGT AGTTCAAGAG GTAAAAAATT TACGCTCTGG AGTTTCTCGT 
GCTGCTGTGG TCTGTTTAAG TGATCTTTTT ACTCACCTGA AAAAGAGCAT GGATCAAGAA 
CTAGATAATA CCGTAAAAGT TTTATTGCAG AAGTCAGGAG AATCAAATAC ATTTATAAGA 
GAAGATGTTG ACAAGGCATT AAGAGCTATG ATCACTAATG TAACTCCCGC ACGTGCGGTT 
GTTTCTCTTA TCAACGGAGG ACAAAGCCAC TTACACATTG CAGTAAGAAG ATGTGCTGCC 
CAGCATTTAT CAGATGTGGT AGAATTTATG GAACCAGAGC GTGTTTTATC TGGAACAAAG 
GATATGGCTG ACCGTATATT ACCCGCTGCT GCTAAATTTG CTCAGGACAG CTCACCAGAA 
ACCAGGTATT ATGGCCGAAA GATGCTTTTC CTCATGATGT GTCATCCTAA CTTTGATAAA 
ATGCTTGAAA AGTACGTGCC ATCTAAAGAT TTGCCATATA TTAAGGAATC TGTTAAAAGC 
TTACGGCAAA AGGGTTTGGG AGAGATACCG CTAGATACTC CTTCAGCAAA AGGAAGACGA 
TCTCATACCG GCAGTGTTGG AAACACAAGG TCGTCATCTG TTTCTAGGGA TGCTTTCAAT 
TCAGCTGAAA GAGAGGTAAC TGAAATTCGT GAAATCACCA GAAAATCAGT TCCCCGAAAT 
TCCTTAGAAA GTGCTGAGTA CATTAAAGTC ATAACAGGTT TATTAAATGC AAAAGATTTT 
CGTGATCGTA TTAATGGGAT TAAGCAGCTT TTATCAGATA CAGAAAACAA TCAAGAGCTT 
GTTGTTGGAA ACATTGTGAA GATCTTTGAT GCTTTTAAAT CTCGGCTTCA TGATTCCAAT 
AGTAAAGTAA ACCTGGTGGC TCTAGAAACA ATGCACAAAA TGATTCCTTT GCTCAGAGAC 
AACTTATCTC CTATAATCAA CATGCTAATC CCAGCAATAG TAGATAACAA TCTGAATTCC 
AAGAATCCAG GCATCTATGC TGCTGCCACC AATGTTGTTC AGGCACTGAG TCAGCACGTA 
GATAATTACT TACTTCTACA GCCATTTTGC ACAAAAGCCC AGTTTTTAAA TGGAAAGGCA 
AAACAGGATA TGACTGAAAA GCTTGCTGAT ATTGTTATGG AACTTTATCA AAGGAAGCCC 
CATGCAACAG AACAGAAAGT GTTGGTTGTT TTATGGCATC TCTTAGGGAA CATGACAAAT 
AGTGGCTCTT TGCCTGGAGC TGGAGGAAAT ATACGAACAG CCACAGCTAA ATTATCAAAA 
GCACTTTTTA CACAGATGGG TCAGAATCTG TTAAATCAGG CTGCATCTCA ACCATCACAT 
ATAAAAAAGA GTTTAGAAGA ATTGCTTGAT ATGACAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_024982094.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837862.1 Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X5, mRNA.

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CloneID	OBa228504
Clone ID Related Accession (Same CDS sequence)	XM_024982094.1
Accession Version	XM_024982094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TOG array regulator of axonemal microtubules protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACATG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGCATC TACAGGGTCA 
TCATCAAATT TCCACCAAAT TTCCAGTTTT GACTTCATGT CTACAAATAC CTTATCAGAA 
GACTCAGTAG TAGTTGTTGG AAAAGGGGTA TTTGGAAATC CAATTCCAGC ACCAGCAACC 
TGCAGTCAAT CAGTGATAAC TTCTGTGGAA AATGGGGTTT CGTTTTCAGT TAAAAATACT 
GAACCACCAT CAGGCATTTA TGGAAGAGCA GTTCAGCAGA GTATTCCATC ATATGTTGAT 
TCTGAGAATG AAAAAGATAT TAAAGTTTCT ATTTCAAAAT CTACTTATGA CAAGATGAGA 
CAAAAGAGAA AAGAGGAAAA AGAACTTTTT GACATTAAAG ATTCTGAAAG GAAGGAACAG 
AATCCCTGGG AACGAATGAA ATATACAGGA AATGAAAAAA TGGCACCTCA AAATGAAGCA 
TCTCCTGGAG CCATTCCACA GTATAAAGAA AGGATGCCCT CTGTCACTCA TAGTCCAGAA 
ATAATGGATT CATCAGAACT ACGGCCATTT TCCAAACCCG AAGTAGCACT GACAGAAGCT 
CTGAGGCTTT TAGCTGATGA GGATTGGGAG AAGAAAATTG AGGGACTGAA CTTTATTAGA 
TGTTTAGCTG CTTTTCATTC TGAAATACTG AACACAAAGT TGCATGAAAC AAATTTTGCA 
GTAGTTCAAG AGGTAAAAAA TTTACGCTCT GGAGTTTCTC GTGCTGCTGT GGTCTGTTTA 
AGTGATCTTT TTACTCACCT GAAAAAGAGC ATGGATCAAG AACTAGATAA TACCGTAAAA 
GTTTTATTGC AGAAGTCAGG AGAATCAAAT ACATTTATAA GAGAAGATGT TGACAAGGCA 
TTAAGAGCTA TGATCACTAA TGTAACTCCC GCACGTGCGG TTGTTTCTCT TATCAACGGA 
GGACAAAGCC ACTTACACAT TGCAGTAAGA AGATGTGCTG CCCAGCATTT ATCAGATGTG 
GTAGAATTTA TGGAACCAGA GCGTGTTTTA TCTGGAACAA AGGATATGGC TGACCGTATA 
TTACCCGCTG CTGCTAAATT TGCTCAGGAC AGCTCACCAG AAACCAGGTA TTATGGCCGA 
AAGATGCTTT TCCTCATGAT GTGTCATCCT AACTTTGATA AAATGCTTGA AAAGTACGTG 
CCATCTAAAG ATTTGCCATA TATTAAGGAA TCTGTTAAAA GCTTACGGCA AAAGGGTTTG 
GGAGAGATAC CGCTAGATAC TCCTTCAGCA AAAGGAAGAC GATCTCATAC CGGCAGTGTT 
GGAAACACAA GGTCGTCATC TGTTTCTAGG GATGCTTTCA ATTCAGCTGA AAGAGAGGTA 
ACTGAAATTC GTGAAATCAC CAGAAAATCA GTTCCCCGAA ATTCCTTAGA AAGTGCTGAG 
TACATTAAAG TCATAACAGG TTTATTAAAT GCAAAAGATT TTCGTGATCG TATTAATGGG 
ATTAAGCAGC TTTTATCAGA TACAGAAAAC AATCAAGAGC TTGTTGTTGG AAACATTGTG 
AAGATCTTTG ATGCTTTTAA ATCTCGGCTT CATGATTCCA ATAGTAAAGT AAACCTGGTG 
GCTCTAGAAA CAATGCACAA AATGATTCCT TTGCTCAGAG ACAACTTATC TCCTATAATC 
AACATGCTAA TCCCAGCAAT AGTAGATAAC AATCTGAATT CCAAGAATCC AGGCATCTAT 
GCTGCTGCCA CCAATGTTGT TCAGGCACTG AGTCAGCACG TAGATAATTA CTTACTTCTA 
CAGCCATTTT GCACAAAAGC CCAGTTTTTA AATGGAAAGG CAAAACAGGA TATGACTGAA 
AAGCTTGCTG ATATTGTTAT GGAACTTTAT CAAAGGAAGC CCCATGCAAC AGAACAGAAA 
GTGTTGGTTG TTTTATGGCA TCTCTTAGGG AACATGACAA ATAGTGGCTC TTTGCCTGGA 
GCTGGAGGAA ATATACGAAC AGCCACAGCT AAATTATCAA AAGCACTTTT TACACAGATG 
GGTCAGAATC TGTTAAATCA GGCTGCATCT CAACCATCAC ATATAAAAAA GAGTTTAGAA 
GAATTGCTTG ATATGACAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837862.1
CDS	245..5548
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAGTSGTHQTNLSGKCGQLGFSQICGKTGSVDSDLQYLGTTNSHQDKVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHMSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQNEASPGAIPQYKERMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIFDAFKSRLHDSNSKVNLVALETMHKMIPLLRDNLSPIINMLIPAIVDNNLNSKNPGIYAAATNVVQALSQHVDNYLLLQPFCTKAQFLNGKAKQDMTEKLADIVMELYQRKPHATEQKVLVVLWHLLGNMTNSGSLPGAGGNIRTATAKLSKALFTQMGQNLLNQAASQPSHIKKSLEELLDMTI

Target ORF information:

RefSeq Version	XM_024982094.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982094.1

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACATG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGCATC TACAGGGTCA 
TCATCAAATT TCCACCAAAT TTCCAGTTTT GACTTCATGT CTACAAATAC CTTATCAGAA 
GACTCAGTAG TAGTTGTTGG AAAAGGGGTA TTTGGAAATC CAATTCCAGC ACCAGCAACC 
TGCAGTCAAT CAGTGATAAC TTCTGTGGAA AATGGGGTTT CGTTTTCAGT TAAAAATACT 
GAACCACCAT CAGGCATTTA TGGAAGAGCA GTTCAGCAGA GTATTCCATC ATATGTTGAT 
TCTGAGAATG AAAAAGATAT TAAAGTTTCT ATTTCAAAAT CTACTTATGA CAAGATGAGA 
CAAAAGAGAA AAGAGGAAAA AGAACTTTTT GACATTAAAG ATTCTGAAAG GAAGGAACAG 
AATCCCTGGG AACGAATGAA ATATACAGGA AATGAAAAAA TGGCACCTCA AAATGAAGCA 
TCTCCTGGAG CCATTCCACA GTATAAAGAA AGGATGCCCT CTGTCACTCA TAGTCCAGAA 
ATAATGGATT CATCAGAACT ACGGCCATTT TCCAAACCCG AAGTAGCACT GACAGAAGCT 
CTGAGGCTTT TAGCTGATGA GGATTGGGAG AAGAAAATTG AGGGACTGAA CTTTATTAGA 
TGTTTAGCTG CTTTTCATTC TGAAATACTG AACACAAAGT TGCATGAAAC AAATTTTGCA 
GTAGTTCAAG AGGTAAAAAA TTTACGCTCT GGAGTTTCTC GTGCTGCTGT GGTCTGTTTA 
AGTGATCTTT TTACTCACCT GAAAAAGAGC ATGGATCAAG AACTAGATAA TACCGTAAAA 
GTTTTATTGC AGAAGTCAGG AGAATCAAAT ACATTTATAA GAGAAGATGT TGACAAGGCA 
TTAAGAGCTA TGATCACTAA TGTAACTCCC GCACGTGCGG TTGTTTCTCT TATCAACGGA 
GGACAAAGCC ACTTACACAT TGCAGTAAGA AGATGTGCTG CCCAGCATTT ATCAGATGTG 
GTAGAATTTA TGGAACCAGA GCGTGTTTTA TCTGGAACAA AGGATATGGC TGACCGTATA 
TTACCCGCTG CTGCTAAATT TGCTCAGGAC AGCTCACCAG AAACCAGGTA TTATGGCCGA 
AAGATGCTTT TCCTCATGAT GTGTCATCCT AACTTTGATA AAATGCTTGA AAAGTACGTG 
CCATCTAAAG ATTTGCCATA TATTAAGGAA TCTGTTAAAA GCTTACGGCA AAAGGGTTTG 
GGAGAGATAC CGCTAGATAC TCCTTCAGCA AAAGGAAGAC GATCTCATAC CGGCAGTGTT 
GGAAACACAA GGTCGTCATC TGTTTCTAGG GATGCTTTCA ATTCAGCTGA AAGAGAGGTA 
ACTGAAATTC GTGAAATCAC CAGAAAATCA GTTCCCCGAA ATTCCTTAGA AAGTGCTGAG 
TACATTAAAG TCATAACAGG TTTATTAAAT GCAAAAGATT TTCGTGATCG TATTAATGGG 
ATTAAGCAGC TTTTATCAGA TACAGAAAAC AATCAAGAGC TTGTTGTTGG AAACATTGTG 
AAGATCTTTG ATGCTTTTAA ATCTCGGCTT CATGATTCCA ATAGTAAAGT AAACCTGGTG 
GCTCTAGAAA CAATGCACAA AATGATTCCT TTGCTCAGAG ACAACTTATC TCCTATAATC 
AACATGCTAA TCCCAGCAAT AGTAGATAAC AATCTGAATT CCAAGAATCC AGGCATCTAT 
GCTGCTGCCA CCAATGTTGT TCAGGCACTG AGTCAGCACG TAGATAATTA CTTACTTCTA 
CAGCCATTTT GCACAAAAGC CCAGTTTTTA AATGGAAAGG CAAAACAGGA TATGACTGAA 
AAGCTTGCTG ATATTGTTAT GGAACTTTAT CAAAGGAAGC CCCATGCAAC AGAACAGAAA 
GTGTTGGTTG TTTTATGGCA TCTCTTAGGG AACATGACAA ATAGTGGCTC TTTGCCTGGA 
GCTGGAGGAA ATATACGAAC AGCCACAGCT AAATTATCAA AAGCACTTTT TACACAGATG 
GGTCAGAATC TGTTAAATCA GGCTGCATCT CAACCATCAC ATATAAAAAA GAGTTTAGAA 
GAATTGCTTG ATATGACAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_010817290.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815592.2 Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X6, mRNA.

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CloneID	OBa228505
Clone ID Related Accession (Same CDS sequence)	XM_010817290.3
Accession Version	XM_010817290.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	TOG array regulator of axonemal microtubules protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817290.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACATG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAATATGT TTCTTTGTTC TAGATGAAGC ATCTCCTGGA 
GCCATTCCAC AGTATAAAGA AAGGATGCCC TCTGTCACTC ATAGTCCAGA AATAATGGAT 
TCATCAGAAC TACGGCCATT TTCCAAACCC GAAGTAGCAC TGACAGAAGC TCTGAGGCTT 
TTAGCTGATG AGGATTGGGA GAAGAAAATT GAGGGACTGA ACTTTATTAG ATGTTTAGCT 
GCTTTTCATT CTGAAATACT GAACACAAAG TTGCATGAAA CAAATTTTGC AGTAGTTCAA 
GAGGTAAAAA ATTTACGCTC TGGAGTTTCT CGTGCTGCTG TGGTCTGTTT AAGTGATCTT 
TTTACTCACC TGAAAAAGAG CATGGATCAA GAACTAGATA ATACCGTAAA AGTTTTATTG 
CAGAAGTCAG GAGAATCAAA TACATTTATA AGAGAAGATG TTGACAAGGC ATTAAGAGCT 
ATGATCACTA ATGTAACTCC CGCACGTGCG GTTGTTTCTC TTATCAACGG AGGACAAAGC 
CACTTACACA TTGCAGTAAG AAGATGTGCT GCCCAGCATT TATCAGATGT GGTAGAATTT 
ATGGAACCAG AGCGTGTTTT ATCTGGAACA AAGGATATGG CTGACCGTAT ATTACCCGCT 
GCTGCTAAAT TTGCTCAGGA CAGCTCACCA GAAACCAGGT ATTATGGCCG AAAGATGCTT 
TTCCTCATGA TGTGTCATCC TAACTTTGAT AAAATGCTTG AAAAGTACGT GCCATCTAAA 
GATTTGCCAT ATATTAAGGA ATCTGTTAAA AGCTTACGGC AAAAGGGTTT GGGAGAGATA 
CCGCTAGATA CTCCTTCAGC AAAAGGAAGA CGATCTCATA CCGGCAGTGT TGGAAACACA 
AGGTCGTCAT CTGTTTCTAG GGATGCTTTC AATTCAGCTG AAAGAGAGGT AACTGAAATT 
CGTGAAATCA CCAGAAAATC AGTTCCCCGA AATTCCTTAG AAAGTGCTGA GTACATTAAA 
GTCATAACAG GTTTATTAAA TGCAAAAGAT TTTCGTGATC GTATTAATGG GATTAAGCAG 
CTTTTATCAG ATACAGAAAA CAATCAAGAG CTTGTTGTTG GAAACATTGT GAAGATAATT 
ACTTACTTCT ACAGCCATTT TGCACAAAAG CCCAGTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815592.2
CDS	199..5160
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAGTSGTHQTNLSGKCGQLGFSQICGKTGSVDSDLQYLGTTNSHQDKVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHMSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTGTTTSSVSIIGHRMNYGFGCGDFEEDRSHDISPSALKIQNKEQPRHYKHTKASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQICFFVLDEASPGAIPQYKERMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIITYFYSHFAQKPSF

Target ORF information:

RefSeq Version	XM_010817290.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TOG array regulator of axonemal microtubules 1 (TOGARAM1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817290.3

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACATG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAATATGT TTCTTTGTTC TAGATGAAGC ATCTCCTGGA 
GCCATTCCAC AGTATAAAGA AAGGATGCCC TCTGTCACTC ATAGTCCAGA AATAATGGAT 
TCATCAGAAC TACGGCCATT TTCCAAACCC GAAGTAGCAC TGACAGAAGC TCTGAGGCTT 
TTAGCTGATG AGGATTGGGA GAAGAAAATT GAGGGACTGA ACTTTATTAG ATGTTTAGCT 
GCTTTTCATT CTGAAATACT GAACACAAAG TTGCATGAAA CAAATTTTGC AGTAGTTCAA 
GAGGTAAAAA ATTTACGCTC TGGAGTTTCT CGTGCTGCTG TGGTCTGTTT AAGTGATCTT 
TTTACTCACC TGAAAAAGAG CATGGATCAA GAACTAGATA ATACCGTAAA AGTTTTATTG 
CAGAAGTCAG GAGAATCAAA TACATTTATA AGAGAAGATG TTGACAAGGC ATTAAGAGCT 
ATGATCACTA ATGTAACTCC CGCACGTGCG GTTGTTTCTC TTATCAACGG AGGACAAAGC 
CACTTACACA TTGCAGTAAG AAGATGTGCT GCCCAGCATT TATCAGATGT GGTAGAATTT 
ATGGAACCAG AGCGTGTTTT ATCTGGAACA AAGGATATGG CTGACCGTAT ATTACCCGCT 
GCTGCTAAAT TTGCTCAGGA CAGCTCACCA GAAACCAGGT ATTATGGCCG AAAGATGCTT 
TTCCTCATGA TGTGTCATCC TAACTTTGAT AAAATGCTTG AAAAGTACGT GCCATCTAAA 
GATTTGCCAT ATATTAAGGA ATCTGTTAAA AGCTTACGGC AAAAGGGTTT GGGAGAGATA 
CCGCTAGATA CTCCTTCAGC AAAAGGAAGA CGATCTCATA CCGGCAGTGT TGGAAACACA 
AGGTCGTCAT CTGTTTCTAG GGATGCTTTC AATTCAGCTG AAAGAGAGGT AACTGAAATT 
CGTGAAATCA CCAGAAAATC AGTTCCCCGA AATTCCTTAG AAAGTGCTGA GTACATTAAA 
GTCATAACAG GTTTATTAAA TGCAAAAGAT TTTCGTGATC GTATTAATGG GATTAAGCAG 
CTTTTATCAG ATACAGAAAA CAATCAAGAG CTTGTTGTTG GAAACATTGT GAAGATAATT 
ACTTACTTCT ACAGCCATTT TGCACAAAAG CCCAGTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_010817286.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815588.1 Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X1, mRNA.

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CloneID	OBa54592
Clone ID Related Accession (Same CDS sequence)	XM_010817286.2 , XM_010824922.2
Accession Version	XM_010817286.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein FAM179B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817286.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAATATGT TTCTTTGTTC TAGATGAAGC ATCTCCTGGA 
GCCATTCCAC AGTATAAACA AAGGATGCCC TCTGTCACTC ATAGTCCAGA AATAATGGAT 
TCATCAGAAC TACGGCCATT TTCCAAACCC GAAGTAGCAC TGACAGAAGC TCTGAGGCTT 
TTAGCTGATG AGGATTGGGA GAAGAAAATT GAGGGACTGA ACTTTATTAG ATGTTTAGCT 
GCTTTTCATT CTGAAATACT GAACACAAAG TTGCATGAAA CAAATTTTGC AGTAGTTCAA 
GAGGTAAAAA ATTTACGCTC TGGAGTTTCT CGTGCTGCTG TGGTCTGTTT AAGTGATCTT 
TTTACTCACC TGAAAAAGAG CATGGATCAA GAACTAGATA ATACCGTAAA AGTTTTATTG 
CAGAAGTCAG GAGAATCAAA TACATTTATA AGAGAAGATG TTGACAAGGC ATTAAGAGCT 
ATGATCACTA ATGTAACTCC CGCACGTGCG GTTGTTTCTC TTATCAACGG AGGACAAAGC 
CACTTACACA TTGCAGTAAG AAGATGTGCT GCCCAGCATT TATCAGATGT GGTAGAATTT 
ATGGAACCAG AGCGTGTTTT ATCTGGAACA AAGGATATGG CTGACCGTAT ATTACCCGCT 
GCTGCTAAAT TTGCTCAGGA CAGCTCACCA GAAACCAGGT ATTATGGCCG AAAGATGCTT 
TTCCTCATGA TGTGTCATCC TAACTTTGAT AAAATGCTTG AAAAGTACGT GCCATCTAAA 
GATTTGCCAT ATATTAAGGA ATCTGTTAAA AGCTTACGGC AAAAGGGTTT GGGAGAGATA 
CCGCTAGATA CTCCTTCAGC AAAAGGAAGA CGATCTCATA CCGGCAGTGT TGGAAACACA 
AGGTCGTCAT CTGTTTCTAG GGATGCTTTC AATTCAGCTG AAAGAGAGGT AACTGAAATT 
CGTGAAATCA CCAGAAAATC AGTTCCCCGA AATTCCTTAG AAAGTGCTGA GTACATTAAA 
GTCATAACAG GTTTATTAAA TGCAAAAGAT TTTCGTGATC GTATTAATGG GATTAAGCAG 
CTTTTATCAG ATACAGAAAA CAATCAAGAG CTTGTTGTTG GAAACATTGT GAAGATCTTT 
GATGCTTTTA AATCTCGGCT TCATGATTCC AATAGTAAAG TAAACCTGGT GGCTCTAGAA 
ACAATGCACA AAATGATTCC TTTGCTCAGA GACAACTTAT CTCCTATAAT CAACATGCTA 
ATCCCAGCAA TAGTAGATAA CAATCTGAAT TCCAAGAATC CAGGCATCTA TGCTGCTGCC 
ACCAATGTTG TTCAGGCACT GAGTCAGCAC GTAGATAATT ACTTACTTCT ACAGCCATTT 
TGCACAAAAG CCCAGTTTTT AAATGGAAAG GCAAAACAGG ATATGACTGA AAAGCTTGCT 
GATATTGTTA TGGAACTTTA TCAAAGGAAG CCCCATGCAA CAGAACAGAA AGTGTTGGTT 
GTTTTATGGC ATCTCTTAGG GAACATGACA AATAGTGGCT CTTTGCCTGG AGCTGGAGGA 
AATATACGAA CAGCCACAGC TAAATTATCA AAAGCACTTT TTACACAGAT GGGTCAGAAT 
CTGTTAAATC AGGCTGCATC TCAACCATCA CATATAAAAA AGAGTTTAGA AGAATTGCTT 
GATATGACAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815588.1
CDS	174..5648
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAGTSGTHQTNLSGKCGQLGFSQICGKTGSVDSDLQYLGTTNSHQDKVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHTSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTGTTTSSVSIIGHRMNYGFGCGDFEEDRSHDISPSALKIQNKEQPRHYKHTKASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQICFFVLDEASPGAIPQYKQRMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIFDAFKSRLHDSNSKVNLVALETMHKMIPLLRDNLSPIINMLIPAIVDNNLNSKNPGIYAAATNVVQALSQHVDNYLLLQPFCTKAQFLNGKAKQDMTEKLADIVMELYQRKPHATEQKVLVVLWHLLGNMTNSGSLPGAGGNIRTATAKLSKALFTQMGQNLLNQAASQPSHIKKSLEELLDMTI

Target ORF information:

RefSeq Version	XM_010817286.2
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817286.2

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAATATGT TTCTTTGTTC TAGATGAAGC ATCTCCTGGA 
GCCATTCCAC AGTATAAACA AAGGATGCCC TCTGTCACTC ATAGTCCAGA AATAATGGAT 
TCATCAGAAC TACGGCCATT TTCCAAACCC GAAGTAGCAC TGACAGAAGC TCTGAGGCTT 
TTAGCTGATG AGGATTGGGA GAAGAAAATT GAGGGACTGA ACTTTATTAG ATGTTTAGCT 
GCTTTTCATT CTGAAATACT GAACACAAAG TTGCATGAAA CAAATTTTGC AGTAGTTCAA 
GAGGTAAAAA ATTTACGCTC TGGAGTTTCT CGTGCTGCTG TGGTCTGTTT AAGTGATCTT 
TTTACTCACC TGAAAAAGAG CATGGATCAA GAACTAGATA ATACCGTAAA AGTTTTATTG 
CAGAAGTCAG GAGAATCAAA TACATTTATA AGAGAAGATG TTGACAAGGC ATTAAGAGCT 
ATGATCACTA ATGTAACTCC CGCACGTGCG GTTGTTTCTC TTATCAACGG AGGACAAAGC 
CACTTACACA TTGCAGTAAG AAGATGTGCT GCCCAGCATT TATCAGATGT GGTAGAATTT 
ATGGAACCAG AGCGTGTTTT ATCTGGAACA AAGGATATGG CTGACCGTAT ATTACCCGCT 
GCTGCTAAAT TTGCTCAGGA CAGCTCACCA GAAACCAGGT ATTATGGCCG AAAGATGCTT 
TTCCTCATGA TGTGTCATCC TAACTTTGAT AAAATGCTTG AAAAGTACGT GCCATCTAAA 
GATTTGCCAT ATATTAAGGA ATCTGTTAAA AGCTTACGGC AAAAGGGTTT GGGAGAGATA 
CCGCTAGATA CTCCTTCAGC AAAAGGAAGA CGATCTCATA CCGGCAGTGT TGGAAACACA 
AGGTCGTCAT CTGTTTCTAG GGATGCTTTC AATTCAGCTG AAAGAGAGGT AACTGAAATT 
CGTGAAATCA CCAGAAAATC AGTTCCCCGA AATTCCTTAG AAAGTGCTGA GTACATTAAA 
GTCATAACAG GTTTATTAAA TGCAAAAGAT TTTCGTGATC GTATTAATGG GATTAAGCAG 
CTTTTATCAG ATACAGAAAA CAATCAAGAG CTTGTTGTTG GAAACATTGT GAAGATCTTT 
GATGCTTTTA AATCTCGGCT TCATGATTCC AATAGTAAAG TAAACCTGGT GGCTCTAGAA 
ACAATGCACA AAATGATTCC TTTGCTCAGA GACAACTTAT CTCCTATAAT CAACATGCTA 
ATCCCAGCAA TAGTAGATAA CAATCTGAAT TCCAAGAATC CAGGCATCTA TGCTGCTGCC 
ACCAATGTTG TTCAGGCACT GAGTCAGCAC GTAGATAATT ACTTACTTCT ACAGCCATTT 
TGCACAAAAG CCCAGTTTTT AAATGGAAAG GCAAAACAGG ATATGACTGA AAAGCTTGCT 
GATATTGTTA TGGAACTTTA TCAAAGGAAG CCCCATGCAA CAGAACAGAA AGTGTTGGTT 
GTTTTATGGC ATCTCTTAGG GAACATGACA AATAGTGGCT CTTTGCCTGG AGCTGGAGGA 
AATATACGAA CAGCCACAGC TAAATTATCA AAAGCACTTT TTACACAGAT GGGTCAGAAT 
CTGTTAAATC AGGCTGCATC TCAACCATCA CATATAAAAA AGAGTTTAGA AGAATTGCTT 
GATATGACAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_010824922.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823224.1 Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X1, mRNA.

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CloneID	OBa54592
Clone ID Related Accession (Same CDS sequence)	XM_010817286.2 , XM_010824922.2
Accession Version	XM_010824922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein FAM179B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646539.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAATATGT TTCTTTGTTC TAGATGAAGC ATCTCCTGGA 
GCCATTCCAC AGTATAAACA AAGGATGCCC TCTGTCACTC ATAGTCCAGA AATAATGGAT 
TCATCAGAAC TACGGCCATT TTCCAAACCC GAAGTAGCAC TGACAGAAGC TCTGAGGCTT 
TTAGCTGATG AGGATTGGGA GAAGAAAATT GAGGGACTGA ACTTTATTAG ATGTTTAGCT 
GCTTTTCATT CTGAAATACT GAACACAAAG TTGCATGAAA CAAATTTTGC AGTAGTTCAA 
GAGGTAAAAA ATTTACGCTC TGGAGTTTCT CGTGCTGCTG TGGTCTGTTT AAGTGATCTT 
TTTACTCACC TGAAAAAGAG CATGGATCAA GAACTAGATA ATACCGTAAA AGTTTTATTG 
CAGAAGTCAG GAGAATCAAA TACATTTATA AGAGAAGATG TTGACAAGGC ATTAAGAGCT 
ATGATCACTA ATGTAACTCC CGCACGTGCG GTTGTTTCTC TTATCAACGG AGGACAAAGC 
CACTTACACA TTGCAGTAAG AAGATGTGCT GCCCAGCATT TATCAGATGT GGTAGAATTT 
ATGGAACCAG AGCGTGTTTT ATCTGGAACA AAGGATATGG CTGACCGTAT ATTACCCGCT 
GCTGCTAAAT TTGCTCAGGA CAGCTCACCA GAAACCAGGT ATTATGGCCG AAAGATGCTT 
TTCCTCATGA TGTGTCATCC TAACTTTGAT AAAATGCTTG AAAAGTACGT GCCATCTAAA 
GATTTGCCAT ATATTAAGGA ATCTGTTAAA AGCTTACGGC AAAAGGGTTT GGGAGAGATA 
CCGCTAGATA CTCCTTCAGC AAAAGGAAGA CGATCTCATA CCGGCAGTGT TGGAAACACA 
AGGTCGTCAT CTGTTTCTAG GGATGCTTTC AATTCAGCTG AAAGAGAGGT AACTGAAATT 
CGTGAAATCA CCAGAAAATC AGTTCCCCGA AATTCCTTAG AAAGTGCTGA GTACATTAAA 
GTCATAACAG GTTTATTAAA TGCAAAAGAT TTTCGTGATC GTATTAATGG GATTAAGCAG 
CTTTTATCAG ATACAGAAAA CAATCAAGAG CTTGTTGTTG GAAACATTGT GAAGATCTTT 
GATGCTTTTA AATCTCGGCT TCATGATTCC AATAGTAAAG TAAACCTGGT GGCTCTAGAA 
ACAATGCACA AAATGATTCC TTTGCTCAGA GACAACTTAT CTCCTATAAT CAACATGCTA 
ATCCCAGCAA TAGTAGATAA CAATCTGAAT TCCAAGAATC CAGGCATCTA TGCTGCTGCC 
ACCAATGTTG TTCAGGCACT GAGTCAGCAC GTAGATAATT ACTTACTTCT ACAGCCATTT 
TGCACAAAAG CCCAGTTTTT AAATGGAAAG GCAAAACAGG ATATGACTGA AAAGCTTGCT 
GATATTGTTA TGGAACTTTA TCAAAGGAAG CCCCATGCAA CAGAACAGAA AGTGTTGGTT 
GTTTTATGGC ATCTCTTAGG GAACATGACA AATAGTGGCT CTTTGCCTGG AGCTGGAGGA 
AATATACGAA CAGCCACAGC TAAATTATCA AAAGCACTTT TTACACAGAT GGGTCAGAAT 
CTGTTAAATC AGGCTGCATC TCAACCATCA CATATAAAAA AGAGTTTAGA AGAATTGCTT 
GATATGACAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823224.1
CDS	174..5648
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAGTSGTHQTNLSGKCGQLGFSQICGKTGSVDSDLQYLGTTNSHQDKVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHTSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTGTTTSSVSIIGHRMNYGFGCGDFEEDRSHDISPSALKIQNKEQPRHYKHTKASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQICFFVLDEASPGAIPQYKQRMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIFDAFKSRLHDSNSKVNLVALETMHKMIPLLRDNLSPIINMLIPAIVDNNLNSKNPGIYAAATNVVQALSQHVDNYLLLQPFCTKAQFLNGKAKQDMTEKLADIVMELYQRKPHATEQKVLVVLWHLLGNMTNSGSLPGAGGNIRTATAKLSKALFTQMGQNLLNQAASQPSHIKKSLEELLDMTI

Target ORF information:

RefSeq Version	XM_010824922.2
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824922.2

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAATATGT TTCTTTGTTC TAGATGAAGC ATCTCCTGGA 
GCCATTCCAC AGTATAAACA AAGGATGCCC TCTGTCACTC ATAGTCCAGA AATAATGGAT 
TCATCAGAAC TACGGCCATT TTCCAAACCC GAAGTAGCAC TGACAGAAGC TCTGAGGCTT 
TTAGCTGATG AGGATTGGGA GAAGAAAATT GAGGGACTGA ACTTTATTAG ATGTTTAGCT 
GCTTTTCATT CTGAAATACT GAACACAAAG TTGCATGAAA CAAATTTTGC AGTAGTTCAA 
GAGGTAAAAA ATTTACGCTC TGGAGTTTCT CGTGCTGCTG TGGTCTGTTT AAGTGATCTT 
TTTACTCACC TGAAAAAGAG CATGGATCAA GAACTAGATA ATACCGTAAA AGTTTTATTG 
CAGAAGTCAG GAGAATCAAA TACATTTATA AGAGAAGATG TTGACAAGGC ATTAAGAGCT 
ATGATCACTA ATGTAACTCC CGCACGTGCG GTTGTTTCTC TTATCAACGG AGGACAAAGC 
CACTTACACA TTGCAGTAAG AAGATGTGCT GCCCAGCATT TATCAGATGT GGTAGAATTT 
ATGGAACCAG AGCGTGTTTT ATCTGGAACA AAGGATATGG CTGACCGTAT ATTACCCGCT 
GCTGCTAAAT TTGCTCAGGA CAGCTCACCA GAAACCAGGT ATTATGGCCG AAAGATGCTT 
TTCCTCATGA TGTGTCATCC TAACTTTGAT AAAATGCTTG AAAAGTACGT GCCATCTAAA 
GATTTGCCAT ATATTAAGGA ATCTGTTAAA AGCTTACGGC AAAAGGGTTT GGGAGAGATA 
CCGCTAGATA CTCCTTCAGC AAAAGGAAGA CGATCTCATA CCGGCAGTGT TGGAAACACA 
AGGTCGTCAT CTGTTTCTAG GGATGCTTTC AATTCAGCTG AAAGAGAGGT AACTGAAATT 
CGTGAAATCA CCAGAAAATC AGTTCCCCGA AATTCCTTAG AAAGTGCTGA GTACATTAAA 
GTCATAACAG GTTTATTAAA TGCAAAAGAT TTTCGTGATC GTATTAATGG GATTAAGCAG 
CTTTTATCAG ATACAGAAAA CAATCAAGAG CTTGTTGTTG GAAACATTGT GAAGATCTTT 
GATGCTTTTA AATCTCGGCT TCATGATTCC AATAGTAAAG TAAACCTGGT GGCTCTAGAA 
ACAATGCACA AAATGATTCC TTTGCTCAGA GACAACTTAT CTCCTATAAT CAACATGCTA 
ATCCCAGCAA TAGTAGATAA CAATCTGAAT TCCAAGAATC CAGGCATCTA TGCTGCTGCC 
ACCAATGTTG TTCAGGCACT GAGTCAGCAC GTAGATAATT ACTTACTTCT ACAGCCATTT 
TGCACAAAAG CCCAGTTTTT AAATGGAAAG GCAAAACAGG ATATGACTGA AAAGCTTGCT 
GATATTGTTA TGGAACTTTA TCAAAGGAAG CCCCATGCAA CAGAACAGAA AGTGTTGGTT 
GTTTTATGGC ATCTCTTAGG GAACATGACA AATAGTGGCT CTTTGCCTGG AGCTGGAGGA 
AATATACGAA CAGCCACAGC TAAATTATCA AAAGCACTTT TTACACAGAT GGGTCAGAAT 
CTGTTAAATC AGGCTGCATC TCAACCATCA CATATAAAAA AGAGTTTAGA AGAATTGCTT 
GATATGACAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_010817287.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815589.1 Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X2, mRNA.

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CloneID	OBa54593
Clone ID Related Accession (Same CDS sequence)	XM_010817287.2 , XM_010824923.2
Accession Version	XM_010817287.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein FAM179B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAAATGAA GCATCTCCTG GAGCCATTCC ACAGTATAAA 
CAAAGGATGC CCTCTGTCAC TCATAGTCCA GAAATAATGG ATTCATCAGA ACTACGGCCA 
TTTTCCAAAC CCGAAGTAGC ACTGACAGAA GCTCTGAGGC TTTTAGCTGA TGAGGATTGG 
GAGAAGAAAA TTGAGGGACT GAACTTTATT AGATGTTTAG CTGCTTTTCA TTCTGAAATA 
CTGAACACAA AGTTGCATGA AACAAATTTT GCAGTAGTTC AAGAGGTAAA AAATTTACGC 
TCTGGAGTTT CTCGTGCTGC TGTGGTCTGT TTAAGTGATC TTTTTACTCA CCTGAAAAAG 
AGCATGGATC AAGAACTAGA TAATACCGTA AAAGTTTTAT TGCAGAAGTC AGGAGAATCA 
AATACATTTA TAAGAGAAGA TGTTGACAAG GCATTAAGAG CTATGATCAC TAATGTAACT 
CCCGCACGTG CGGTTGTTTC TCTTATCAAC GGAGGACAAA GCCACTTACA CATTGCAGTA 
AGAAGATGTG CTGCCCAGCA TTTATCAGAT GTGGTAGAAT TTATGGAACC AGAGCGTGTT 
TTATCTGGAA CAAAGGATAT GGCTGACCGT ATATTACCCG CTGCTGCTAA ATTTGCTCAG 
GACAGCTCAC CAGAAACCAG GTATTATGGC CGAAAGATGC TTTTCCTCAT GATGTGTCAT 
CCTAACTTTG ATAAAATGCT TGAAAAGTAC GTGCCATCTA AAGATTTGCC ATATATTAAG 
GAATCTGTTA AAAGCTTACG GCAAAAGGGT TTGGGAGAGA TACCGCTAGA TACTCCTTCA 
GCAAAAGGAA GACGATCTCA TACCGGCAGT GTTGGAAACA CAAGGTCGTC ATCTGTTTCT 
AGGGATGCTT TCAATTCAGC TGAAAGAGAG GTAACTGAAA TTCGTGAAAT CACCAGAAAA 
TCAGTTCCCC GAAATTCCTT AGAAAGTGCT GAGTACATTA AAGTCATAAC AGGTTTATTA 
AATGCAAAAG ATTTTCGTGA TCGTATTAAT GGGATTAAGC AGCTTTTATC AGATACAGAA 
AACAATCAAG AGCTTGTTGT TGGAAACATT GTGAAGATCT TTGATGCTTT TAAATCTCGG 
CTTCATGATT CCAATAGTAA AGTAAACCTG GTGGCTCTAG AAACAATGCA CAAAATGATT 
CCTTTGCTCA GAGACAACTT ATCTCCTATA ATCAACATGC TAATCCCAGC AATAGTAGAT 
AACAATCTGA ATTCCAAGAA TCCAGGCATC TATGCTGCTG CCACCAATGT TGTTCAGGCA 
CTGAGTCAGC ACGTAGATAA TTACTTACTT CTACAGCCAT TTTGCACAAA AGCCCAGTTT 
TTAAATGGAA AGGCAAAACA GGATATGACT GAAAAGCTTG CTGATATTGT TATGGAACTT 
TATCAAAGGA AGCCCCATGC AACAGAACAG AAAGTGTTGG TTGTTTTATG GCATCTCTTA 
GGGAACATGA CAAATAGTGG CTCTTTGCCT GGAGCTGGAG GAAATATACG AACAGCCACA 
GCTAAATTAT CAAAAGCACT TTTTACACAG ATGGGTCAGA ATCTGTTAAA TCAGGCTGCA 
TCTCAACCAT CACATATAAA AAAGAGTTTA GAAGAATTGC TTGATATGAC AATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815589.1
CDS	174..5630
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAGTSGTHQTNLSGKCGQLGFSQICGKTGSVDSDLQYLGTTNSHQDKVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHTSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTGTTTSSVSIIGHRMNYGFGCGDFEEDRSHDISPSALKIQNKEQPRHYKHTKASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQNEASPGAIPQYKQRMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIFDAFKSRLHDSNSKVNLVALETMHKMIPLLRDNLSPIINMLIPAIVDNNLNSKNPGIYAAATNVVQALSQHVDNYLLLQPFCTKAQFLNGKAKQDMTEKLADIVMELYQRKPHATEQKVLVVLWHLLGNMTNSGSLPGAGGNIRTATAKLSKALFTQMGQNLLNQAASQPSHIKKSLEELLDMTI

Target ORF information:

RefSeq Version	XM_010817287.2
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817287.2

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAAATGAA GCATCTCCTG GAGCCATTCC ACAGTATAAA 
CAAAGGATGC CCTCTGTCAC TCATAGTCCA GAAATAATGG ATTCATCAGA ACTACGGCCA 
TTTTCCAAAC CCGAAGTAGC ACTGACAGAA GCTCTGAGGC TTTTAGCTGA TGAGGATTGG 
GAGAAGAAAA TTGAGGGACT GAACTTTATT AGATGTTTAG CTGCTTTTCA TTCTGAAATA 
CTGAACACAA AGTTGCATGA AACAAATTTT GCAGTAGTTC AAGAGGTAAA AAATTTACGC 
TCTGGAGTTT CTCGTGCTGC TGTGGTCTGT TTAAGTGATC TTTTTACTCA CCTGAAAAAG 
AGCATGGATC AAGAACTAGA TAATACCGTA AAAGTTTTAT TGCAGAAGTC AGGAGAATCA 
AATACATTTA TAAGAGAAGA TGTTGACAAG GCATTAAGAG CTATGATCAC TAATGTAACT 
CCCGCACGTG CGGTTGTTTC TCTTATCAAC GGAGGACAAA GCCACTTACA CATTGCAGTA 
AGAAGATGTG CTGCCCAGCA TTTATCAGAT GTGGTAGAAT TTATGGAACC AGAGCGTGTT 
TTATCTGGAA CAAAGGATAT GGCTGACCGT ATATTACCCG CTGCTGCTAA ATTTGCTCAG 
GACAGCTCAC CAGAAACCAG GTATTATGGC CGAAAGATGC TTTTCCTCAT GATGTGTCAT 
CCTAACTTTG ATAAAATGCT TGAAAAGTAC GTGCCATCTA AAGATTTGCC ATATATTAAG 
GAATCTGTTA AAAGCTTACG GCAAAAGGGT TTGGGAGAGA TACCGCTAGA TACTCCTTCA 
GCAAAAGGAA GACGATCTCA TACCGGCAGT GTTGGAAACA CAAGGTCGTC ATCTGTTTCT 
AGGGATGCTT TCAATTCAGC TGAAAGAGAG GTAACTGAAA TTCGTGAAAT CACCAGAAAA 
TCAGTTCCCC GAAATTCCTT AGAAAGTGCT GAGTACATTA AAGTCATAAC AGGTTTATTA 
AATGCAAAAG ATTTTCGTGA TCGTATTAAT GGGATTAAGC AGCTTTTATC AGATACAGAA 
AACAATCAAG AGCTTGTTGT TGGAAACATT GTGAAGATCT TTGATGCTTT TAAATCTCGG 
CTTCATGATT CCAATAGTAA AGTAAACCTG GTGGCTCTAG AAACAATGCA CAAAATGATT 
CCTTTGCTCA GAGACAACTT ATCTCCTATA ATCAACATGC TAATCCCAGC AATAGTAGAT 
AACAATCTGA ATTCCAAGAA TCCAGGCATC TATGCTGCTG CCACCAATGT TGTTCAGGCA 
CTGAGTCAGC ACGTAGATAA TTACTTACTT CTACAGCCAT TTTGCACAAA AGCCCAGTTT 
TTAAATGGAA AGGCAAAACA GGATATGACT GAAAAGCTTG CTGATATTGT TATGGAACTT 
TATCAAAGGA AGCCCCATGC AACAGAACAG AAAGTGTTGG TTGTTTTATG GCATCTCTTA 
GGGAACATGA CAAATAGTGG CTCTTTGCCT GGAGCTGGAG GAAATATACG AACAGCCACA 
GCTAAATTAT CAAAAGCACT TTTTACACAG ATGGGTCAGA ATCTGTTAAA TCAGGCTGCA 
TCTCAACCAT CACATATAAA AAAGAGTTTA GAAGAATTGC TTGATATGAC AATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_010824923.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823225.1 Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X2, mRNA.

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CloneID	OBa54593
Clone ID Related Accession (Same CDS sequence)	XM_010817287.2 , XM_010824923.2
Accession Version	XM_010824923.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein FAM179B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646539.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824923.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAAATGAA GCATCTCCTG GAGCCATTCC ACAGTATAAA 
CAAAGGATGC CCTCTGTCAC TCATAGTCCA GAAATAATGG ATTCATCAGA ACTACGGCCA 
TTTTCCAAAC CCGAAGTAGC ACTGACAGAA GCTCTGAGGC TTTTAGCTGA TGAGGATTGG 
GAGAAGAAAA TTGAGGGACT GAACTTTATT AGATGTTTAG CTGCTTTTCA TTCTGAAATA 
CTGAACACAA AGTTGCATGA AACAAATTTT GCAGTAGTTC AAGAGGTAAA AAATTTACGC 
TCTGGAGTTT CTCGTGCTGC TGTGGTCTGT TTAAGTGATC TTTTTACTCA CCTGAAAAAG 
AGCATGGATC AAGAACTAGA TAATACCGTA AAAGTTTTAT TGCAGAAGTC AGGAGAATCA 
AATACATTTA TAAGAGAAGA TGTTGACAAG GCATTAAGAG CTATGATCAC TAATGTAACT 
CCCGCACGTG CGGTTGTTTC TCTTATCAAC GGAGGACAAA GCCACTTACA CATTGCAGTA 
AGAAGATGTG CTGCCCAGCA TTTATCAGAT GTGGTAGAAT TTATGGAACC AGAGCGTGTT 
TTATCTGGAA CAAAGGATAT GGCTGACCGT ATATTACCCG CTGCTGCTAA ATTTGCTCAG 
GACAGCTCAC CAGAAACCAG GTATTATGGC CGAAAGATGC TTTTCCTCAT GATGTGTCAT 
CCTAACTTTG ATAAAATGCT TGAAAAGTAC GTGCCATCTA AAGATTTGCC ATATATTAAG 
GAATCTGTTA AAAGCTTACG GCAAAAGGGT TTGGGAGAGA TACCGCTAGA TACTCCTTCA 
GCAAAAGGAA GACGATCTCA TACCGGCAGT GTTGGAAACA CAAGGTCGTC ATCTGTTTCT 
AGGGATGCTT TCAATTCAGC TGAAAGAGAG GTAACTGAAA TTCGTGAAAT CACCAGAAAA 
TCAGTTCCCC GAAATTCCTT AGAAAGTGCT GAGTACATTA AAGTCATAAC AGGTTTATTA 
AATGCAAAAG ATTTTCGTGA TCGTATTAAT GGGATTAAGC AGCTTTTATC AGATACAGAA 
AACAATCAAG AGCTTGTTGT TGGAAACATT GTGAAGATCT TTGATGCTTT TAAATCTCGG 
CTTCATGATT CCAATAGTAA AGTAAACCTG GTGGCTCTAG AAACAATGCA CAAAATGATT 
CCTTTGCTCA GAGACAACTT ATCTCCTATA ATCAACATGC TAATCCCAGC AATAGTAGAT 
AACAATCTGA ATTCCAAGAA TCCAGGCATC TATGCTGCTG CCACCAATGT TGTTCAGGCA 
CTGAGTCAGC ACGTAGATAA TTACTTACTT CTACAGCCAT TTTGCACAAA AGCCCAGTTT 
TTAAATGGAA AGGCAAAACA GGATATGACT GAAAAGCTTG CTGATATTGT TATGGAACTT 
TATCAAAGGA AGCCCCATGC AACAGAACAG AAAGTGTTGG TTGTTTTATG GCATCTCTTA 
GGGAACATGA CAAATAGTGG CTCTTTGCCT GGAGCTGGAG GAAATATACG AACAGCCACA 
GCTAAATTAT CAAAAGCACT TTTTACACAG ATGGGTCAGA ATCTGTTAAA TCAGGCTGCA 
TCTCAACCAT CACATATAAA AAAGAGTTTA GAAGAATTGC TTGATATGAC AATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823225.1
CDS	174..5630
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAGTSGTHQTNLSGKCGQLGFSQICGKTGSVDSDLQYLGTTNSHQDKVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHTSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTGTTTSSVSIIGHRMNYGFGCGDFEEDRSHDISPSALKIQNKEQPRHYKHTKASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQNEASPGAIPQYKQRMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIFDAFKSRLHDSNSKVNLVALETMHKMIPLLRDNLSPIINMLIPAIVDNNLNSKNPGIYAAATNVVQALSQHVDNYLLLQPFCTKAQFLNGKAKQDMTEKLADIVMELYQRKPHATEQKVLVVLWHLLGNMTNSGSLPGAGGNIRTATAKLSKALFTQMGQNLLNQAASQPSHIKKSLEELLDMTI

Target ORF information:

RefSeq Version	XM_010824923.2
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824923.2

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAAATGAA GCATCTCCTG GAGCCATTCC ACAGTATAAA 
CAAAGGATGC CCTCTGTCAC TCATAGTCCA GAAATAATGG ATTCATCAGA ACTACGGCCA 
TTTTCCAAAC CCGAAGTAGC ACTGACAGAA GCTCTGAGGC TTTTAGCTGA TGAGGATTGG 
GAGAAGAAAA TTGAGGGACT GAACTTTATT AGATGTTTAG CTGCTTTTCA TTCTGAAATA 
CTGAACACAA AGTTGCATGA AACAAATTTT GCAGTAGTTC AAGAGGTAAA AAATTTACGC 
TCTGGAGTTT CTCGTGCTGC TGTGGTCTGT TTAAGTGATC TTTTTACTCA CCTGAAAAAG 
AGCATGGATC AAGAACTAGA TAATACCGTA AAAGTTTTAT TGCAGAAGTC AGGAGAATCA 
AATACATTTA TAAGAGAAGA TGTTGACAAG GCATTAAGAG CTATGATCAC TAATGTAACT 
CCCGCACGTG CGGTTGTTTC TCTTATCAAC GGAGGACAAA GCCACTTACA CATTGCAGTA 
AGAAGATGTG CTGCCCAGCA TTTATCAGAT GTGGTAGAAT TTATGGAACC AGAGCGTGTT 
TTATCTGGAA CAAAGGATAT GGCTGACCGT ATATTACCCG CTGCTGCTAA ATTTGCTCAG 
GACAGCTCAC CAGAAACCAG GTATTATGGC CGAAAGATGC TTTTCCTCAT GATGTGTCAT 
CCTAACTTTG ATAAAATGCT TGAAAAGTAC GTGCCATCTA AAGATTTGCC ATATATTAAG 
GAATCTGTTA AAAGCTTACG GCAAAAGGGT TTGGGAGAGA TACCGCTAGA TACTCCTTCA 
GCAAAAGGAA GACGATCTCA TACCGGCAGT GTTGGAAACA CAAGGTCGTC ATCTGTTTCT 
AGGGATGCTT TCAATTCAGC TGAAAGAGAG GTAACTGAAA TTCGTGAAAT CACCAGAAAA 
TCAGTTCCCC GAAATTCCTT AGAAAGTGCT GAGTACATTA AAGTCATAAC AGGTTTATTA 
AATGCAAAAG ATTTTCGTGA TCGTATTAAT GGGATTAAGC AGCTTTTATC AGATACAGAA 
AACAATCAAG AGCTTGTTGT TGGAAACATT GTGAAGATCT TTGATGCTTT TAAATCTCGG 
CTTCATGATT CCAATAGTAA AGTAAACCTG GTGGCTCTAG AAACAATGCA CAAAATGATT 
CCTTTGCTCA GAGACAACTT ATCTCCTATA ATCAACATGC TAATCCCAGC AATAGTAGAT 
AACAATCTGA ATTCCAAGAA TCCAGGCATC TATGCTGCTG CCACCAATGT TGTTCAGGCA 
CTGAGTCAGC ACGTAGATAA TTACTTACTT CTACAGCCAT TTTGCACAAA AGCCCAGTTT 
TTAAATGGAA AGGCAAAACA GGATATGACT GAAAAGCTTG CTGATATTGT TATGGAACTT 
TATCAAAGGA AGCCCCATGC AACAGAACAG AAAGTGTTGG TTGTTTTATG GCATCTCTTA 
GGGAACATGA CAAATAGTGG CTCTTTGCCT GGAGCTGGAG GAAATATACG AACAGCCACA 
GCTAAATTAT CAAAAGCACT TTTTACACAG ATGGGTCAGA ATCTGTTAAA TCAGGCTGCA 
TCTCAACCAT CACATATAAA AAAGAGTTTA GAAGAATTGC TTGATATGAC AATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_010824924.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823226.1 Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X3, mRNA.

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CloneID	OBa54594
Clone ID Related Accession (Same CDS sequence)	XM_010824924.2 , XM_010817288.2
Accession Version	XM_010824924.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein FAM179B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646539.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824924.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGTTTGTGC TAGCCTATGT 
TTTGGTAGTA AGACACAGCA GACATTTGGT AGTCAAACAG AGCGTTCGTC ATACTCTGGT 
CCAAATTCAA GTTCAGGAGC TGTTCTTCTT CCATCCTATC CTCTCTCGTC ACCTCGAACT 
AGTCCAAAGC ACACGTCTCT TACTGTGTCT CCAAAGAAGT CTCAAGATAA TTCTGTTAAT 
TTCTCAAGTT CCTGGCCTCT TAAAAGCTTT GAAGGGCTTC CAAAGCCAAG TCCACAGAAA 
AAGCTTGTCA GCCAAAAATC ATCTGATCCT ACAGGAGAAA ATTCTCAAGA GAAACCTCCT 
CAAGTTCAAC TTACTCCTGC TTTGGTGAGA TCACCGTCTT CCCGACGAGG CTTAAATGGG 
ACCAGACCTG TACCTCCCAT ACCACGGGGA CTCAGCCTTC TGCCTGATAA AGCTGGTCTA 
AGCACAGTGG GACCCAAAAA GAAAGAGCCT GATGACATTT GGAAGAGTGA AAAAGATAAT 
CTTACAGTTG ATCTTTCAGA ATTAAATATC AGGGATAAAG ATTTGGATCA AGAAGAGATG 
CAGAGCTCTC TTAGGTCCCT TCGTAATAGC GCAGCTAAGA AAAGAGCAAA ACTGAGTGGC 
AGTACTTCTG ATCTTGAAAG CCCAGATTCC GCCATGAAGC TTGACTTGAC TGTGGATTCC 
CCATCTCGAT CTTCCTCTCC AAATACCAGT TCCTACAGTG AAAGTGGAGT TTACAGCCAA 
GAATCCTTGA CTTCTTCTCT GTCGACAACT CCCCAGGGGA AGAGAATAAT GTCAGACATA 
TTTCCAACTT TTGGATCAAA ACCTTGTCCA ACAAGACTTT CTTCAGCGAA GAATAAGAAC 
TCTCAGGTAG CTGAACACAG CCCCAGTACA GGGACTACAA CATCCAGTGT AAGCATAATT 
GGTCATCGTA TGAACTATGG GTTTGGATGT GGTGACTTTG AAGAGGATAG GTCACATGAC 
ATTTCACCTA GTGCTTTAAA AATACAAAAT AAGGAACAAC CAAGACATTA TAAGCATACG 
AAAGCATCTA CAGGGTCATC ATCAAATTTC CACCAAATTT CCAGTTTTGA CTTCATGTCT 
ACAAATACCT TATCAGAAGA CTCAGTAGTA GTTGTTGGAA AAGGGGTATT TGGAAATCCA 
ATTCCAGCAC CAGCAACCTG CAGTCAATCA GTGATAACTT CTGTGGAAAA TGGGGTTTCG 
TTTTCAGTTA AAAATACTGA ACCACCATCA GGCATTTATG GAAGAGCAGT TCAGCAGAGT 
ATTCCATCAT ATGTTGATTC TGAGAATGAA AAAGATATTA AAGTTTCTAT TTCAAAATCT 
ACTTATGACA AGATGAGACA AAAGAGAAAA GAGGAAAAAG AACTTTTTGA CATTAAAGAT 
TCTGAAAGGA AGGAACAGAA TCCCTGGGAA CGAATGAAAT ATACAGGAAA TGAAAAAATG 
GCACCTCAAA TATGTTTCTT TGTTCTAGAT GAAGCATCTC CTGGAGCCAT TCCACAGTAT 
AAACAAAGGA TGCCCTCTGT CACTCATAGT CCAGAAATAA TGGATTCATC AGAACTACGG 
CCATTTTCCA AACCCGAAGT AGCACTGACA GAAGCTCTGA GGCTTTTAGC TGATGAGGAT 
TGGGAGAAGA AAATTGAGGG ACTGAACTTT ATTAGATGTT TAGCTGCTTT TCATTCTGAA 
ATACTGAACA CAAAGTTGCA TGAAACAAAT TTTGCAGTAG TTCAAGAGGT AAAAAATTTA 
CGCTCTGGAG TTTCTCGTGC TGCTGTGGTC TGTTTAAGTG ATCTTTTTAC TCACCTGAAA 
AAGAGCATGG ATCAAGAACT AGATAATACC GTAAAAGTTT TATTGCAGAA GTCAGGAGAA 
TCAAATACAT TTATAAGAGA AGATGTTGAC AAGGCATTAA GAGCTATGAT CACTAATGTA 
ACTCCCGCAC GTGCGGTTGT TTCTCTTATC AACGGAGGAC AAAGCCACTT ACACATTGCA 
GTAAGAAGAT GTGCTGCCCA GCATTTATCA GATGTGGTAG AATTTATGGA ACCAGAGCGT 
GTTTTATCTG GAACAAAGGA TATGGCTGAC CGTATATTAC CCGCTGCTGC TAAATTTGCT 
CAGGACAGCT CACCAGAAAC CAGGTATTAT GGCCGAAAGA TGCTTTTCCT CATGATGTGT 
CATCCTAACT TTGATAAAAT GCTTGAAAAG TACGTGCCAT CTAAAGATTT GCCATATATT 
AAGGAATCTG TTAAAAGCTT ACGGCAAAAG GGTTTGGGAG AGATACCGCT AGATACTCCT 
TCAGCAAAAG GAAGACGATC TCATACCGGC AGTGTTGGAA ACACAAGGTC GTCATCTGTT 
TCTAGGGATG CTTTCAATTC AGCTGAAAGA GAGGTAACTG AAATTCGTGA AATCACCAGA 
AAATCAGTTC CCCGAAATTC CTTAGAAAGT GCTGAGTACA TTAAAGTCAT AACAGGTTTA 
TTAAATGCAA AAGATTTTCG TGATCGTATT AATGGGATTA AGCAGCTTTT ATCAGATACA 
GAAAACAATC AAGAGCTTGT TGTTGGAAAC ATTGTGAAGA TCTTTGATGC TTTTAAATCT 
CGGCTTCATG ATTCCAATAG TAAAGTAAAC CTGGTGGCTC TAGAAACAAT GCACAAAATG 
ATTCCTTTGC TCAGAGACAA CTTATCTCCT ATAATCAACA TGCTAATCCC AGCAATAGTA 
GATAACAATC TGAATTCCAA GAATCCAGGC ATCTATGCTG CTGCCACCAA TGTTGTTCAG 
GCACTGAGTC AGCACGTAGA TAATTACTTA CTTCTACAGC CATTTTGCAC AAAAGCCCAG 
TTTTTAAATG GAAAGGCAAA ACAGGATATG ACTGAAAAGC TTGCTGATAT TGTTATGGAA 
CTTTATCAAA GGAAGCCCCA TGCAACAGAA CAGAAAGTGT TGGTTGTTTT ATGGCATCTC 
TTAGGGAACA TGACAAATAG TGGCTCTTTG CCTGGAGCTG GAGGAAATAT ACGAACAGCC 
ACAGCTAAAT TATCAAAAGC ACTTTTTACA CAGATGGGTC AGAATCTGTT AAATCAGGCT 
GCATCTCAAC CATCACATAT AAAAAAGAGT TTAGAAGAAT TGCTTGATAT GACAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823226.1
CDS	176..5515
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHTSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTGTTTSSVSIIGHRMNYGFGCGDFEEDRSHDISPSALKIQNKEQPRHYKHTKASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQICFFVLDEASPGAIPQYKQRMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIFDAFKSRLHDSNSKVNLVALETMHKMIPLLRDNLSPIINMLIPAIVDNNLNSKNPGIYAAATNVVQALSQHVDNYLLLQPFCTKAQFLNGKAKQDMTEKLADIVMELYQRKPHATEQKVLVVLWHLLGNMTNSGSLPGAGGNIRTATAKLSKALFTQMGQNLLNQAASQPSHIKKSLEELLDMTI

Target ORF information:

RefSeq Version	XM_010824924.2
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824924.2

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGTTTGTGC TAGCCTATGT 
TTTGGTAGTA AGACACAGCA GACATTTGGT AGTCAAACAG AGCGTTCGTC ATACTCTGGT 
CCAAATTCAA GTTCAGGAGC TGTTCTTCTT CCATCCTATC CTCTCTCGTC ACCTCGAACT 
AGTCCAAAGC ACACGTCTCT TACTGTGTCT CCAAAGAAGT CTCAAGATAA TTCTGTTAAT 
TTCTCAAGTT CCTGGCCTCT TAAAAGCTTT GAAGGGCTTC CAAAGCCAAG TCCACAGAAA 
AAGCTTGTCA GCCAAAAATC ATCTGATCCT ACAGGAGAAA ATTCTCAAGA GAAACCTCCT 
CAAGTTCAAC TTACTCCTGC TTTGGTGAGA TCACCGTCTT CCCGACGAGG CTTAAATGGG 
ACCAGACCTG TACCTCCCAT ACCACGGGGA CTCAGCCTTC TGCCTGATAA AGCTGGTCTA 
AGCACAGTGG GACCCAAAAA GAAAGAGCCT GATGACATTT GGAAGAGTGA AAAAGATAAT 
CTTACAGTTG ATCTTTCAGA ATTAAATATC AGGGATAAAG ATTTGGATCA AGAAGAGATG 
CAGAGCTCTC TTAGGTCCCT TCGTAATAGC GCAGCTAAGA AAAGAGCAAA ACTGAGTGGC 
AGTACTTCTG ATCTTGAAAG CCCAGATTCC GCCATGAAGC TTGACTTGAC TGTGGATTCC 
CCATCTCGAT CTTCCTCTCC AAATACCAGT TCCTACAGTG AAAGTGGAGT TTACAGCCAA 
GAATCCTTGA CTTCTTCTCT GTCGACAACT CCCCAGGGGA AGAGAATAAT GTCAGACATA 
TTTCCAACTT TTGGATCAAA ACCTTGTCCA ACAAGACTTT CTTCAGCGAA GAATAAGAAC 
TCTCAGGTAG CTGAACACAG CCCCAGTACA GGGACTACAA CATCCAGTGT AAGCATAATT 
GGTCATCGTA TGAACTATGG GTTTGGATGT GGTGACTTTG AAGAGGATAG GTCACATGAC 
ATTTCACCTA GTGCTTTAAA AATACAAAAT AAGGAACAAC CAAGACATTA TAAGCATACG 
AAAGCATCTA CAGGGTCATC ATCAAATTTC CACCAAATTT CCAGTTTTGA CTTCATGTCT 
ACAAATACCT TATCAGAAGA CTCAGTAGTA GTTGTTGGAA AAGGGGTATT TGGAAATCCA 
ATTCCAGCAC CAGCAACCTG CAGTCAATCA GTGATAACTT CTGTGGAAAA TGGGGTTTCG 
TTTTCAGTTA AAAATACTGA ACCACCATCA GGCATTTATG GAAGAGCAGT TCAGCAGAGT 
ATTCCATCAT ATGTTGATTC TGAGAATGAA AAAGATATTA AAGTTTCTAT TTCAAAATCT 
ACTTATGACA AGATGAGACA AAAGAGAAAA GAGGAAAAAG AACTTTTTGA CATTAAAGAT 
TCTGAAAGGA AGGAACAGAA TCCCTGGGAA CGAATGAAAT ATACAGGAAA TGAAAAAATG 
GCACCTCAAA TATGTTTCTT TGTTCTAGAT GAAGCATCTC CTGGAGCCAT TCCACAGTAT 
AAACAAAGGA TGCCCTCTGT CACTCATAGT CCAGAAATAA TGGATTCATC AGAACTACGG 
CCATTTTCCA AACCCGAAGT AGCACTGACA GAAGCTCTGA GGCTTTTAGC TGATGAGGAT 
TGGGAGAAGA AAATTGAGGG ACTGAACTTT ATTAGATGTT TAGCTGCTTT TCATTCTGAA 
ATACTGAACA CAAAGTTGCA TGAAACAAAT TTTGCAGTAG TTCAAGAGGT AAAAAATTTA 
CGCTCTGGAG TTTCTCGTGC TGCTGTGGTC TGTTTAAGTG ATCTTTTTAC TCACCTGAAA 
AAGAGCATGG ATCAAGAACT AGATAATACC GTAAAAGTTT TATTGCAGAA GTCAGGAGAA 
TCAAATACAT TTATAAGAGA AGATGTTGAC AAGGCATTAA GAGCTATGAT CACTAATGTA 
ACTCCCGCAC GTGCGGTTGT TTCTCTTATC AACGGAGGAC AAAGCCACTT ACACATTGCA 
GTAAGAAGAT GTGCTGCCCA GCATTTATCA GATGTGGTAG AATTTATGGA ACCAGAGCGT 
GTTTTATCTG GAACAAAGGA TATGGCTGAC CGTATATTAC CCGCTGCTGC TAAATTTGCT 
CAGGACAGCT CACCAGAAAC CAGGTATTAT GGCCGAAAGA TGCTTTTCCT CATGATGTGT 
CATCCTAACT TTGATAAAAT GCTTGAAAAG TACGTGCCAT CTAAAGATTT GCCATATATT 
AAGGAATCTG TTAAAAGCTT ACGGCAAAAG GGTTTGGGAG AGATACCGCT AGATACTCCT 
TCAGCAAAAG GAAGACGATC TCATACCGGC AGTGTTGGAA ACACAAGGTC GTCATCTGTT 
TCTAGGGATG CTTTCAATTC AGCTGAAAGA GAGGTAACTG AAATTCGTGA AATCACCAGA 
AAATCAGTTC CCCGAAATTC CTTAGAAAGT GCTGAGTACA TTAAAGTCAT AACAGGTTTA 
TTAAATGCAA AAGATTTTCG TGATCGTATT AATGGGATTA AGCAGCTTTT ATCAGATACA 
GAAAACAATC AAGAGCTTGT TGTTGGAAAC ATTGTGAAGA TCTTTGATGC TTTTAAATCT 
CGGCTTCATG ATTCCAATAG TAAAGTAAAC CTGGTGGCTC TAGAAACAAT GCACAAAATG 
ATTCCTTTGC TCAGAGACAA CTTATCTCCT ATAATCAACA TGCTAATCCC AGCAATAGTA 
GATAACAATC TGAATTCCAA GAATCCAGGC ATCTATGCTG CTGCCACCAA TGTTGTTCAG 
GCACTGAGTC AGCACGTAGA TAATTACTTA CTTCTACAGC CATTTTGCAC AAAAGCCCAG 
TTTTTAAATG GAAAGGCAAA ACAGGATATG ACTGAAAAGC TTGCTGATAT TGTTATGGAA 
CTTTATCAAA GGAAGCCCCA TGCAACAGAA CAGAAAGTGT TGGTTGTTTT ATGGCATCTC 
TTAGGGAACA TGACAAATAG TGGCTCTTTG CCTGGAGCTG GAGGAAATAT ACGAACAGCC 
ACAGCTAAAT TATCAAAAGC ACTTTTTACA CAGATGGGTC AGAATCTGTT AAATCAGGCT 
GCATCTCAAC CATCACATAT AAAAAAGAGT TTAGAAGAAT TGCTTGATAT GACAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_010817288.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815590.1 Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X3, mRNA.

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CloneID	OBa54594
Clone ID Related Accession (Same CDS sequence)	XM_010824924.2 , XM_010817288.2
Accession Version	XM_010817288.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein FAM179B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817288.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGTTTGTGC TAGCCTATGT 
TTTGGTAGTA AGACACAGCA GACATTTGGT AGTCAAACAG AGCGTTCGTC ATACTCTGGT 
CCAAATTCAA GTTCAGGAGC TGTTCTTCTT CCATCCTATC CTCTCTCGTC ACCTCGAACT 
AGTCCAAAGC ACACGTCTCT TACTGTGTCT CCAAAGAAGT CTCAAGATAA TTCTGTTAAT 
TTCTCAAGTT CCTGGCCTCT TAAAAGCTTT GAAGGGCTTC CAAAGCCAAG TCCACAGAAA 
AAGCTTGTCA GCCAAAAATC ATCTGATCCT ACAGGAGAAA ATTCTCAAGA GAAACCTCCT 
CAAGTTCAAC TTACTCCTGC TTTGGTGAGA TCACCGTCTT CCCGACGAGG CTTAAATGGG 
ACCAGACCTG TACCTCCCAT ACCACGGGGA CTCAGCCTTC TGCCTGATAA AGCTGGTCTA 
AGCACAGTGG GACCCAAAAA GAAAGAGCCT GATGACATTT GGAAGAGTGA AAAAGATAAT 
CTTACAGTTG ATCTTTCAGA ATTAAATATC AGGGATAAAG ATTTGGATCA AGAAGAGATG 
CAGAGCTCTC TTAGGTCCCT TCGTAATAGC GCAGCTAAGA AAAGAGCAAA ACTGAGTGGC 
AGTACTTCTG ATCTTGAAAG CCCAGATTCC GCCATGAAGC TTGACTTGAC TGTGGATTCC 
CCATCTCGAT CTTCCTCTCC AAATACCAGT TCCTACAGTG AAAGTGGAGT TTACAGCCAA 
GAATCCTTGA CTTCTTCTCT GTCGACAACT CCCCAGGGGA AGAGAATAAT GTCAGACATA 
TTTCCAACTT TTGGATCAAA ACCTTGTCCA ACAAGACTTT CTTCAGCGAA GAATAAGAAC 
TCTCAGGTAG CTGAACACAG CCCCAGTACA GGGACTACAA CATCCAGTGT AAGCATAATT 
GGTCATCGTA TGAACTATGG GTTTGGATGT GGTGACTTTG AAGAGGATAG GTCACATGAC 
ATTTCACCTA GTGCTTTAAA AATACAAAAT AAGGAACAAC CAAGACATTA TAAGCATACG 
AAAGCATCTA CAGGGTCATC ATCAAATTTC CACCAAATTT CCAGTTTTGA CTTCATGTCT 
ACAAATACCT TATCAGAAGA CTCAGTAGTA GTTGTTGGAA AAGGGGTATT TGGAAATCCA 
ATTCCAGCAC CAGCAACCTG CAGTCAATCA GTGATAACTT CTGTGGAAAA TGGGGTTTCG 
TTTTCAGTTA AAAATACTGA ACCACCATCA GGCATTTATG GAAGAGCAGT TCAGCAGAGT 
ATTCCATCAT ATGTTGATTC TGAGAATGAA AAAGATATTA AAGTTTCTAT TTCAAAATCT 
ACTTATGACA AGATGAGACA AAAGAGAAAA GAGGAAAAAG AACTTTTTGA CATTAAAGAT 
TCTGAAAGGA AGGAACAGAA TCCCTGGGAA CGAATGAAAT ATACAGGAAA TGAAAAAATG 
GCACCTCAAA TATGTTTCTT TGTTCTAGAT GAAGCATCTC CTGGAGCCAT TCCACAGTAT 
AAACAAAGGA TGCCCTCTGT CACTCATAGT CCAGAAATAA TGGATTCATC AGAACTACGG 
CCATTTTCCA AACCCGAAGT AGCACTGACA GAAGCTCTGA GGCTTTTAGC TGATGAGGAT 
TGGGAGAAGA AAATTGAGGG ACTGAACTTT ATTAGATGTT TAGCTGCTTT TCATTCTGAA 
ATACTGAACA CAAAGTTGCA TGAAACAAAT TTTGCAGTAG TTCAAGAGGT AAAAAATTTA 
CGCTCTGGAG TTTCTCGTGC TGCTGTGGTC TGTTTAAGTG ATCTTTTTAC TCACCTGAAA 
AAGAGCATGG ATCAAGAACT AGATAATACC GTAAAAGTTT TATTGCAGAA GTCAGGAGAA 
TCAAATACAT TTATAAGAGA AGATGTTGAC AAGGCATTAA GAGCTATGAT CACTAATGTA 
ACTCCCGCAC GTGCGGTTGT TTCTCTTATC AACGGAGGAC AAAGCCACTT ACACATTGCA 
GTAAGAAGAT GTGCTGCCCA GCATTTATCA GATGTGGTAG AATTTATGGA ACCAGAGCGT 
GTTTTATCTG GAACAAAGGA TATGGCTGAC CGTATATTAC CCGCTGCTGC TAAATTTGCT 
CAGGACAGCT CACCAGAAAC CAGGTATTAT GGCCGAAAGA TGCTTTTCCT CATGATGTGT 
CATCCTAACT TTGATAAAAT GCTTGAAAAG TACGTGCCAT CTAAAGATTT GCCATATATT 
AAGGAATCTG TTAAAAGCTT ACGGCAAAAG GGTTTGGGAG AGATACCGCT AGATACTCCT 
TCAGCAAAAG GAAGACGATC TCATACCGGC AGTGTTGGAA ACACAAGGTC GTCATCTGTT 
TCTAGGGATG CTTTCAATTC AGCTGAAAGA GAGGTAACTG AAATTCGTGA AATCACCAGA 
AAATCAGTTC CCCGAAATTC CTTAGAAAGT GCTGAGTACA TTAAAGTCAT AACAGGTTTA 
TTAAATGCAA AAGATTTTCG TGATCGTATT AATGGGATTA AGCAGCTTTT ATCAGATACA 
GAAAACAATC AAGAGCTTGT TGTTGGAAAC ATTGTGAAGA TCTTTGATGC TTTTAAATCT 
CGGCTTCATG ATTCCAATAG TAAAGTAAAC CTGGTGGCTC TAGAAACAAT GCACAAAATG 
ATTCCTTTGC TCAGAGACAA CTTATCTCCT ATAATCAACA TGCTAATCCC AGCAATAGTA 
GATAACAATC TGAATTCCAA GAATCCAGGC ATCTATGCTG CTGCCACCAA TGTTGTTCAG 
GCACTGAGTC AGCACGTAGA TAATTACTTA CTTCTACAGC CATTTTGCAC AAAAGCCCAG 
TTTTTAAATG GAAAGGCAAA ACAGGATATG ACTGAAAAGC TTGCTGATAT TGTTATGGAA 
CTTTATCAAA GGAAGCCCCA TGCAACAGAA CAGAAAGTGT TGGTTGTTTT ATGGCATCTC 
TTAGGGAACA TGACAAATAG TGGCTCTTTG CCTGGAGCTG GAGGAAATAT ACGAACAGCC 
ACAGCTAAAT TATCAAAAGC ACTTTTTACA CAGATGGGTC AGAATCTGTT AAATCAGGCT 
GCATCTCAAC CATCACATAT AAAAAAGAGT TTAGAAGAAT TGCTTGATAT GACAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815590.1
CDS	176..5515
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHTSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTGTTTSSVSIIGHRMNYGFGCGDFEEDRSHDISPSALKIQNKEQPRHYKHTKASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQICFFVLDEASPGAIPQYKQRMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIFDAFKSRLHDSNSKVNLVALETMHKMIPLLRDNLSPIINMLIPAIVDNNLNSKNPGIYAAATNVVQALSQHVDNYLLLQPFCTKAQFLNGKAKQDMTEKLADIVMELYQRKPHATEQKVLVVLWHLLGNMTNSGSLPGAGGNIRTATAKLSKALFTQMGQNLLNQAASQPSHIKKSLEELLDMTI

Target ORF information:

RefSeq Version	XM_010817288.2
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817288.2

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGTTTGTGC TAGCCTATGT 
TTTGGTAGTA AGACACAGCA GACATTTGGT AGTCAAACAG AGCGTTCGTC ATACTCTGGT 
CCAAATTCAA GTTCAGGAGC TGTTCTTCTT CCATCCTATC CTCTCTCGTC ACCTCGAACT 
AGTCCAAAGC ACACGTCTCT TACTGTGTCT CCAAAGAAGT CTCAAGATAA TTCTGTTAAT 
TTCTCAAGTT CCTGGCCTCT TAAAAGCTTT GAAGGGCTTC CAAAGCCAAG TCCACAGAAA 
AAGCTTGTCA GCCAAAAATC ATCTGATCCT ACAGGAGAAA ATTCTCAAGA GAAACCTCCT 
CAAGTTCAAC TTACTCCTGC TTTGGTGAGA TCACCGTCTT CCCGACGAGG CTTAAATGGG 
ACCAGACCTG TACCTCCCAT ACCACGGGGA CTCAGCCTTC TGCCTGATAA AGCTGGTCTA 
AGCACAGTGG GACCCAAAAA GAAAGAGCCT GATGACATTT GGAAGAGTGA AAAAGATAAT 
CTTACAGTTG ATCTTTCAGA ATTAAATATC AGGGATAAAG ATTTGGATCA AGAAGAGATG 
CAGAGCTCTC TTAGGTCCCT TCGTAATAGC GCAGCTAAGA AAAGAGCAAA ACTGAGTGGC 
AGTACTTCTG ATCTTGAAAG CCCAGATTCC GCCATGAAGC TTGACTTGAC TGTGGATTCC 
CCATCTCGAT CTTCCTCTCC AAATACCAGT TCCTACAGTG AAAGTGGAGT TTACAGCCAA 
GAATCCTTGA CTTCTTCTCT GTCGACAACT CCCCAGGGGA AGAGAATAAT GTCAGACATA 
TTTCCAACTT TTGGATCAAA ACCTTGTCCA ACAAGACTTT CTTCAGCGAA GAATAAGAAC 
TCTCAGGTAG CTGAACACAG CCCCAGTACA GGGACTACAA CATCCAGTGT AAGCATAATT 
GGTCATCGTA TGAACTATGG GTTTGGATGT GGTGACTTTG AAGAGGATAG GTCACATGAC 
ATTTCACCTA GTGCTTTAAA AATACAAAAT AAGGAACAAC CAAGACATTA TAAGCATACG 
AAAGCATCTA CAGGGTCATC ATCAAATTTC CACCAAATTT CCAGTTTTGA CTTCATGTCT 
ACAAATACCT TATCAGAAGA CTCAGTAGTA GTTGTTGGAA AAGGGGTATT TGGAAATCCA 
ATTCCAGCAC CAGCAACCTG CAGTCAATCA GTGATAACTT CTGTGGAAAA TGGGGTTTCG 
TTTTCAGTTA AAAATACTGA ACCACCATCA GGCATTTATG GAAGAGCAGT TCAGCAGAGT 
ATTCCATCAT ATGTTGATTC TGAGAATGAA AAAGATATTA AAGTTTCTAT TTCAAAATCT 
ACTTATGACA AGATGAGACA AAAGAGAAAA GAGGAAAAAG AACTTTTTGA CATTAAAGAT 
TCTGAAAGGA AGGAACAGAA TCCCTGGGAA CGAATGAAAT ATACAGGAAA TGAAAAAATG 
GCACCTCAAA TATGTTTCTT TGTTCTAGAT GAAGCATCTC CTGGAGCCAT TCCACAGTAT 
AAACAAAGGA TGCCCTCTGT CACTCATAGT CCAGAAATAA TGGATTCATC AGAACTACGG 
CCATTTTCCA AACCCGAAGT AGCACTGACA GAAGCTCTGA GGCTTTTAGC TGATGAGGAT 
TGGGAGAAGA AAATTGAGGG ACTGAACTTT ATTAGATGTT TAGCTGCTTT TCATTCTGAA 
ATACTGAACA CAAAGTTGCA TGAAACAAAT TTTGCAGTAG TTCAAGAGGT AAAAAATTTA 
CGCTCTGGAG TTTCTCGTGC TGCTGTGGTC TGTTTAAGTG ATCTTTTTAC TCACCTGAAA 
AAGAGCATGG ATCAAGAACT AGATAATACC GTAAAAGTTT TATTGCAGAA GTCAGGAGAA 
TCAAATACAT TTATAAGAGA AGATGTTGAC AAGGCATTAA GAGCTATGAT CACTAATGTA 
ACTCCCGCAC GTGCGGTTGT TTCTCTTATC AACGGAGGAC AAAGCCACTT ACACATTGCA 
GTAAGAAGAT GTGCTGCCCA GCATTTATCA GATGTGGTAG AATTTATGGA ACCAGAGCGT 
GTTTTATCTG GAACAAAGGA TATGGCTGAC CGTATATTAC CCGCTGCTGC TAAATTTGCT 
CAGGACAGCT CACCAGAAAC CAGGTATTAT GGCCGAAAGA TGCTTTTCCT CATGATGTGT 
CATCCTAACT TTGATAAAAT GCTTGAAAAG TACGTGCCAT CTAAAGATTT GCCATATATT 
AAGGAATCTG TTAAAAGCTT ACGGCAAAAG GGTTTGGGAG AGATACCGCT AGATACTCCT 
TCAGCAAAAG GAAGACGATC TCATACCGGC AGTGTTGGAA ACACAAGGTC GTCATCTGTT 
TCTAGGGATG CTTTCAATTC AGCTGAAAGA GAGGTAACTG AAATTCGTGA AATCACCAGA 
AAATCAGTTC CCCGAAATTC CTTAGAAAGT GCTGAGTACA TTAAAGTCAT AACAGGTTTA 
TTAAATGCAA AAGATTTTCG TGATCGTATT AATGGGATTA AGCAGCTTTT ATCAGATACA 
GAAAACAATC AAGAGCTTGT TGTTGGAAAC ATTGTGAAGA TCTTTGATGC TTTTAAATCT 
CGGCTTCATG ATTCCAATAG TAAAGTAAAC CTGGTGGCTC TAGAAACAAT GCACAAAATG 
ATTCCTTTGC TCAGAGACAA CTTATCTCCT ATAATCAACA TGCTAATCCC AGCAATAGTA 
GATAACAATC TGAATTCCAA GAATCCAGGC ATCTATGCTG CTGCCACCAA TGTTGTTCAG 
GCACTGAGTC AGCACGTAGA TAATTACTTA CTTCTACAGC CATTTTGCAC AAAAGCCCAG 
TTTTTAAATG GAAAGGCAAA ACAGGATATG ACTGAAAAGC TTGCTGATAT TGTTATGGAA 
CTTTATCAAA GGAAGCCCCA TGCAACAGAA CAGAAAGTGT TGGTTGTTTT ATGGCATCTC 
TTAGGGAACA TGACAAATAG TGGCTCTTTG CCTGGAGCTG GAGGAAATAT ACGAACAGCC 
ACAGCTAAAT TATCAAAAGC ACTTTTTACA CAGATGGGTC AGAATCTGTT AAATCAGGCT 
GCATCTCAAC CATCACATAT AAAAAAGAGT TTAGAAGAAT TGCTTGATAT GACAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_010817289.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815591.1 Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X4, mRNA.

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CloneID	OBa54595
Clone ID Related Accession (Same CDS sequence)	XM_010817289.2 , XM_010824926.2
Accession Version	XM_010817289.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein FAM179B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817289.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGCATC TACAGGGTCA 
TCATCAAATT TCCACCAAAT TTCCAGTTTT GACTTCATGT CTACAAATAC CTTATCAGAA 
GACTCAGTAG TAGTTGTTGG AAAAGGGGTA TTTGGAAATC CAATTCCAGC ACCAGCAACC 
TGCAGTCAAT CAGTGATAAC TTCTGTGGAA AATGGGGTTT CGTTTTCAGT TAAAAATACT 
GAACCACCAT CAGGCATTTA TGGAAGAGCA GTTCAGCAGA GTATTCCATC ATATGTTGAT 
TCTGAGAATG AAAAAGATAT TAAAGTTTCT ATTTCAAAAT CTACTTATGA CAAGATGAGA 
CAAAAGAGAA AAGAGGAAAA AGAACTTTTT GACATTAAAG ATTCTGAAAG GAAGGAACAG 
AATCCCTGGG AACGAATGAA ATATACAGGA AATGAAAAAA TGGCACCTCA AATATGTTTC 
TTTGTTCTAG ATGAAGCATC TCCTGGAGCC ATTCCACAGT ATAAACAAAG GATGCCCTCT 
GTCACTCATA GTCCAGAAAT AATGGATTCA TCAGAACTAC GGCCATTTTC CAAACCCGAA 
GTAGCACTGA CAGAAGCTCT GAGGCTTTTA GCTGATGAGG ATTGGGAGAA GAAAATTGAG 
GGACTGAACT TTATTAGATG TTTAGCTGCT TTTCATTCTG AAATACTGAA CACAAAGTTG 
CATGAAACAA ATTTTGCAGT AGTTCAAGAG GTAAAAAATT TACGCTCTGG AGTTTCTCGT 
GCTGCTGTGG TCTGTTTAAG TGATCTTTTT ACTCACCTGA AAAAGAGCAT GGATCAAGAA 
CTAGATAATA CCGTAAAAGT TTTATTGCAG AAGTCAGGAG AATCAAATAC ATTTATAAGA 
GAAGATGTTG ACAAGGCATT AAGAGCTATG ATCACTAATG TAACTCCCGC ACGTGCGGTT 
GTTTCTCTTA TCAACGGAGG ACAAAGCCAC TTACACATTG CAGTAAGAAG ATGTGCTGCC 
CAGCATTTAT CAGATGTGGT AGAATTTATG GAACCAGAGC GTGTTTTATC TGGAACAAAG 
GATATGGCTG ACCGTATATT ACCCGCTGCT GCTAAATTTG CTCAGGACAG CTCACCAGAA 
ACCAGGTATT ATGGCCGAAA GATGCTTTTC CTCATGATGT GTCATCCTAA CTTTGATAAA 
ATGCTTGAAA AGTACGTGCC ATCTAAAGAT TTGCCATATA TTAAGGAATC TGTTAAAAGC 
TTACGGCAAA AGGGTTTGGG AGAGATACCG CTAGATACTC CTTCAGCAAA AGGAAGACGA 
TCTCATACCG GCAGTGTTGG AAACACAAGG TCGTCATCTG TTTCTAGGGA TGCTTTCAAT 
TCAGCTGAAA GAGAGGTAAC TGAAATTCGT GAAATCACCA GAAAATCAGT TCCCCGAAAT 
TCCTTAGAAA GTGCTGAGTA CATTAAAGTC ATAACAGGTT TATTAAATGC AAAAGATTTT 
CGTGATCGTA TTAATGGGAT TAAGCAGCTT TTATCAGATA CAGAAAACAA TCAAGAGCTT 
GTTGTTGGAA ACATTGTGAA GATCTTTGAT GCTTTTAAAT CTCGGCTTCA TGATTCCAAT 
AGTAAAGTAA ACCTGGTGGC TCTAGAAACA ATGCACAAAA TGATTCCTTT GCTCAGAGAC 
AACTTATCTC CTATAATCAA CATGCTAATC CCAGCAATAG TAGATAACAA TCTGAATTCC 
AAGAATCCAG GCATCTATGC TGCTGCCACC AATGTTGTTC AGGCACTGAG TCAGCACGTA 
GATAATTACT TACTTCTACA GCCATTTTGC ACAAAAGCCC AGTTTTTAAA TGGAAAGGCA 
AAACAGGATA TGACTGAAAA GCTTGCTGAT ATTGTTATGG AACTTTATCA AAGGAAGCCC 
CATGCAACAG AACAGAAAGT GTTGGTTGTT TTATGGCATC TCTTAGGGAA CATGACAAAT 
AGTGGCTCTT TGCCTGGAGC TGGAGGAAAT ATACGAACAG CCACAGCTAA ATTATCAAAA 
GCACTTTTTA CACAGATGGG TCAGAATCTG TTAAATCAGG CTGCATCTCA ACCATCACAT 
ATAAAAAAGA GTTTAGAAGA ATTGCTTGAT ATGACAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815591.1
CDS	174..5495
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAGTSGTHQTNLSGKCGQLGFSQICGKTGSVDSDLQYLGTTNSHQDKVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHTSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQICFFVLDEASPGAIPQYKQRMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIFDAFKSRLHDSNSKVNLVALETMHKMIPLLRDNLSPIINMLIPAIVDNNLNSKNPGIYAAATNVVQALSQHVDNYLLLQPFCTKAQFLNGKAKQDMTEKLADIVMELYQRKPHATEQKVLVVLWHLLGNMTNSGSLPGAGGNIRTATAKLSKALFTQMGQNLLNQAASQPSHIKKSLEELLDMTI

Target ORF information:

RefSeq Version	XM_010817289.2
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817289.2

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGCATC TACAGGGTCA 
TCATCAAATT TCCACCAAAT TTCCAGTTTT GACTTCATGT CTACAAATAC CTTATCAGAA 
GACTCAGTAG TAGTTGTTGG AAAAGGGGTA TTTGGAAATC CAATTCCAGC ACCAGCAACC 
TGCAGTCAAT CAGTGATAAC TTCTGTGGAA AATGGGGTTT CGTTTTCAGT TAAAAATACT 
GAACCACCAT CAGGCATTTA TGGAAGAGCA GTTCAGCAGA GTATTCCATC ATATGTTGAT 
TCTGAGAATG AAAAAGATAT TAAAGTTTCT ATTTCAAAAT CTACTTATGA CAAGATGAGA 
CAAAAGAGAA AAGAGGAAAA AGAACTTTTT GACATTAAAG ATTCTGAAAG GAAGGAACAG 
AATCCCTGGG AACGAATGAA ATATACAGGA AATGAAAAAA TGGCACCTCA AATATGTTTC 
TTTGTTCTAG ATGAAGCATC TCCTGGAGCC ATTCCACAGT ATAAACAAAG GATGCCCTCT 
GTCACTCATA GTCCAGAAAT AATGGATTCA TCAGAACTAC GGCCATTTTC CAAACCCGAA 
GTAGCACTGA CAGAAGCTCT GAGGCTTTTA GCTGATGAGG ATTGGGAGAA GAAAATTGAG 
GGACTGAACT TTATTAGATG TTTAGCTGCT TTTCATTCTG AAATACTGAA CACAAAGTTG 
CATGAAACAA ATTTTGCAGT AGTTCAAGAG GTAAAAAATT TACGCTCTGG AGTTTCTCGT 
GCTGCTGTGG TCTGTTTAAG TGATCTTTTT ACTCACCTGA AAAAGAGCAT GGATCAAGAA 
CTAGATAATA CCGTAAAAGT TTTATTGCAG AAGTCAGGAG AATCAAATAC ATTTATAAGA 
GAAGATGTTG ACAAGGCATT AAGAGCTATG ATCACTAATG TAACTCCCGC ACGTGCGGTT 
GTTTCTCTTA TCAACGGAGG ACAAAGCCAC TTACACATTG CAGTAAGAAG ATGTGCTGCC 
CAGCATTTAT CAGATGTGGT AGAATTTATG GAACCAGAGC GTGTTTTATC TGGAACAAAG 
GATATGGCTG ACCGTATATT ACCCGCTGCT GCTAAATTTG CTCAGGACAG CTCACCAGAA 
ACCAGGTATT ATGGCCGAAA GATGCTTTTC CTCATGATGT GTCATCCTAA CTTTGATAAA 
ATGCTTGAAA AGTACGTGCC ATCTAAAGAT TTGCCATATA TTAAGGAATC TGTTAAAAGC 
TTACGGCAAA AGGGTTTGGG AGAGATACCG CTAGATACTC CTTCAGCAAA AGGAAGACGA 
TCTCATACCG GCAGTGTTGG AAACACAAGG TCGTCATCTG TTTCTAGGGA TGCTTTCAAT 
TCAGCTGAAA GAGAGGTAAC TGAAATTCGT GAAATCACCA GAAAATCAGT TCCCCGAAAT 
TCCTTAGAAA GTGCTGAGTA CATTAAAGTC ATAACAGGTT TATTAAATGC AAAAGATTTT 
CGTGATCGTA TTAATGGGAT TAAGCAGCTT TTATCAGATA CAGAAAACAA TCAAGAGCTT 
GTTGTTGGAA ACATTGTGAA GATCTTTGAT GCTTTTAAAT CTCGGCTTCA TGATTCCAAT 
AGTAAAGTAA ACCTGGTGGC TCTAGAAACA ATGCACAAAA TGATTCCTTT GCTCAGAGAC 
AACTTATCTC CTATAATCAA CATGCTAATC CCAGCAATAG TAGATAACAA TCTGAATTCC 
AAGAATCCAG GCATCTATGC TGCTGCCACC AATGTTGTTC AGGCACTGAG TCAGCACGTA 
GATAATTACT TACTTCTACA GCCATTTTGC ACAAAAGCCC AGTTTTTAAA TGGAAAGGCA 
AAACAGGATA TGACTGAAAA GCTTGCTGAT ATTGTTATGG AACTTTATCA AAGGAAGCCC 
CATGCAACAG AACAGAAAGT GTTGGTTGTT TTATGGCATC TCTTAGGGAA CATGACAAAT 
AGTGGCTCTT TGCCTGGAGC TGGAGGAAAT ATACGAACAG CCACAGCTAA ATTATCAAAA 
GCACTTTTTA CACAGATGGG TCAGAATCTG TTAAATCAGG CTGCATCTCA ACCATCACAT 
ATAAAAAAGA GTTTAGAAGA ATTGCTTGAT ATGACAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_010824926.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823228.1 Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X4, mRNA.

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CloneID	OBa54595
Clone ID Related Accession (Same CDS sequence)	XM_010817289.2 , XM_010824926.2
Accession Version	XM_010824926.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein FAM179B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646539.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824926.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGCATC TACAGGGTCA 
TCATCAAATT TCCACCAAAT TTCCAGTTTT GACTTCATGT CTACAAATAC CTTATCAGAA 
GACTCAGTAG TAGTTGTTGG AAAAGGGGTA TTTGGAAATC CAATTCCAGC ACCAGCAACC 
TGCAGTCAAT CAGTGATAAC TTCTGTGGAA AATGGGGTTT CGTTTTCAGT TAAAAATACT 
GAACCACCAT CAGGCATTTA TGGAAGAGCA GTTCAGCAGA GTATTCCATC ATATGTTGAT 
TCTGAGAATG AAAAAGATAT TAAAGTTTCT ATTTCAAAAT CTACTTATGA CAAGATGAGA 
CAAAAGAGAA AAGAGGAAAA AGAACTTTTT GACATTAAAG ATTCTGAAAG GAAGGAACAG 
AATCCCTGGG AACGAATGAA ATATACAGGA AATGAAAAAA TGGCACCTCA AATATGTTTC 
TTTGTTCTAG ATGAAGCATC TCCTGGAGCC ATTCCACAGT ATAAACAAAG GATGCCCTCT 
GTCACTCATA GTCCAGAAAT AATGGATTCA TCAGAACTAC GGCCATTTTC CAAACCCGAA 
GTAGCACTGA CAGAAGCTCT GAGGCTTTTA GCTGATGAGG ATTGGGAGAA GAAAATTGAG 
GGACTGAACT TTATTAGATG TTTAGCTGCT TTTCATTCTG AAATACTGAA CACAAAGTTG 
CATGAAACAA ATTTTGCAGT AGTTCAAGAG GTAAAAAATT TACGCTCTGG AGTTTCTCGT 
GCTGCTGTGG TCTGTTTAAG TGATCTTTTT ACTCACCTGA AAAAGAGCAT GGATCAAGAA 
CTAGATAATA CCGTAAAAGT TTTATTGCAG AAGTCAGGAG AATCAAATAC ATTTATAAGA 
GAAGATGTTG ACAAGGCATT AAGAGCTATG ATCACTAATG TAACTCCCGC ACGTGCGGTT 
GTTTCTCTTA TCAACGGAGG ACAAAGCCAC TTACACATTG CAGTAAGAAG ATGTGCTGCC 
CAGCATTTAT CAGATGTGGT AGAATTTATG GAACCAGAGC GTGTTTTATC TGGAACAAAG 
GATATGGCTG ACCGTATATT ACCCGCTGCT GCTAAATTTG CTCAGGACAG CTCACCAGAA 
ACCAGGTATT ATGGCCGAAA GATGCTTTTC CTCATGATGT GTCATCCTAA CTTTGATAAA 
ATGCTTGAAA AGTACGTGCC ATCTAAAGAT TTGCCATATA TTAAGGAATC TGTTAAAAGC 
TTACGGCAAA AGGGTTTGGG AGAGATACCG CTAGATACTC CTTCAGCAAA AGGAAGACGA 
TCTCATACCG GCAGTGTTGG AAACACAAGG TCGTCATCTG TTTCTAGGGA TGCTTTCAAT 
TCAGCTGAAA GAGAGGTAAC TGAAATTCGT GAAATCACCA GAAAATCAGT TCCCCGAAAT 
TCCTTAGAAA GTGCTGAGTA CATTAAAGTC ATAACAGGTT TATTAAATGC AAAAGATTTT 
CGTGATCGTA TTAATGGGAT TAAGCAGCTT TTATCAGATA CAGAAAACAA TCAAGAGCTT 
GTTGTTGGAA ACATTGTGAA GATCTTTGAT GCTTTTAAAT CTCGGCTTCA TGATTCCAAT 
AGTAAAGTAA ACCTGGTGGC TCTAGAAACA ATGCACAAAA TGATTCCTTT GCTCAGAGAC 
AACTTATCTC CTATAATCAA CATGCTAATC CCAGCAATAG TAGATAACAA TCTGAATTCC 
AAGAATCCAG GCATCTATGC TGCTGCCACC AATGTTGTTC AGGCACTGAG TCAGCACGTA 
GATAATTACT TACTTCTACA GCCATTTTGC ACAAAAGCCC AGTTTTTAAA TGGAAAGGCA 
AAACAGGATA TGACTGAAAA GCTTGCTGAT ATTGTTATGG AACTTTATCA AAGGAAGCCC 
CATGCAACAG AACAGAAAGT GTTGGTTGTT TTATGGCATC TCTTAGGGAA CATGACAAAT 
AGTGGCTCTT TGCCTGGAGC TGGAGGAAAT ATACGAACAG CCACAGCTAA ATTATCAAAA 
GCACTTTTTA CACAGATGGG TCAGAATCTG TTAAATCAGG CTGCATCTCA ACCATCACAT 
ATAAAAAAGA GTTTAGAAGA ATTGCTTGAT ATGACAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823228.1
CDS	174..5495
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAGTSGTHQTNLSGKCGQLGFSQICGKTGSVDSDLQYLGTTNSHQDKVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHTSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQICFFVLDEASPGAIPQYKQRMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIFDAFKSRLHDSNSKVNLVALETMHKMIPLLRDNLSPIINMLIPAIVDNNLNSKNPGIYAAATNVVQALSQHVDNYLLLQPFCTKAQFLNGKAKQDMTEKLADIVMELYQRKPHATEQKVLVVLWHLLGNMTNSGSLPGAGGNIRTATAKLSKALFTQMGQNLLNQAASQPSHIKKSLEELLDMTI

Target ORF information:

RefSeq Version	XM_010824926.2
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824926.2

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGCATC TACAGGGTCA 
TCATCAAATT TCCACCAAAT TTCCAGTTTT GACTTCATGT CTACAAATAC CTTATCAGAA 
GACTCAGTAG TAGTTGTTGG AAAAGGGGTA TTTGGAAATC CAATTCCAGC ACCAGCAACC 
TGCAGTCAAT CAGTGATAAC TTCTGTGGAA AATGGGGTTT CGTTTTCAGT TAAAAATACT 
GAACCACCAT CAGGCATTTA TGGAAGAGCA GTTCAGCAGA GTATTCCATC ATATGTTGAT 
TCTGAGAATG AAAAAGATAT TAAAGTTTCT ATTTCAAAAT CTACTTATGA CAAGATGAGA 
CAAAAGAGAA AAGAGGAAAA AGAACTTTTT GACATTAAAG ATTCTGAAAG GAAGGAACAG 
AATCCCTGGG AACGAATGAA ATATACAGGA AATGAAAAAA TGGCACCTCA AATATGTTTC 
TTTGTTCTAG ATGAAGCATC TCCTGGAGCC ATTCCACAGT ATAAACAAAG GATGCCCTCT 
GTCACTCATA GTCCAGAAAT AATGGATTCA TCAGAACTAC GGCCATTTTC CAAACCCGAA 
GTAGCACTGA CAGAAGCTCT GAGGCTTTTA GCTGATGAGG ATTGGGAGAA GAAAATTGAG 
GGACTGAACT TTATTAGATG TTTAGCTGCT TTTCATTCTG AAATACTGAA CACAAAGTTG 
CATGAAACAA ATTTTGCAGT AGTTCAAGAG GTAAAAAATT TACGCTCTGG AGTTTCTCGT 
GCTGCTGTGG TCTGTTTAAG TGATCTTTTT ACTCACCTGA AAAAGAGCAT GGATCAAGAA 
CTAGATAATA CCGTAAAAGT TTTATTGCAG AAGTCAGGAG AATCAAATAC ATTTATAAGA 
GAAGATGTTG ACAAGGCATT AAGAGCTATG ATCACTAATG TAACTCCCGC ACGTGCGGTT 
GTTTCTCTTA TCAACGGAGG ACAAAGCCAC TTACACATTG CAGTAAGAAG ATGTGCTGCC 
CAGCATTTAT CAGATGTGGT AGAATTTATG GAACCAGAGC GTGTTTTATC TGGAACAAAG 
GATATGGCTG ACCGTATATT ACCCGCTGCT GCTAAATTTG CTCAGGACAG CTCACCAGAA 
ACCAGGTATT ATGGCCGAAA GATGCTTTTC CTCATGATGT GTCATCCTAA CTTTGATAAA 
ATGCTTGAAA AGTACGTGCC ATCTAAAGAT TTGCCATATA TTAAGGAATC TGTTAAAAGC 
TTACGGCAAA AGGGTTTGGG AGAGATACCG CTAGATACTC CTTCAGCAAA AGGAAGACGA 
TCTCATACCG GCAGTGTTGG AAACACAAGG TCGTCATCTG TTTCTAGGGA TGCTTTCAAT 
TCAGCTGAAA GAGAGGTAAC TGAAATTCGT GAAATCACCA GAAAATCAGT TCCCCGAAAT 
TCCTTAGAAA GTGCTGAGTA CATTAAAGTC ATAACAGGTT TATTAAATGC AAAAGATTTT 
CGTGATCGTA TTAATGGGAT TAAGCAGCTT TTATCAGATA CAGAAAACAA TCAAGAGCTT 
GTTGTTGGAA ACATTGTGAA GATCTTTGAT GCTTTTAAAT CTCGGCTTCA TGATTCCAAT 
AGTAAAGTAA ACCTGGTGGC TCTAGAAACA ATGCACAAAA TGATTCCTTT GCTCAGAGAC 
AACTTATCTC CTATAATCAA CATGCTAATC CCAGCAATAG TAGATAACAA TCTGAATTCC 
AAGAATCCAG GCATCTATGC TGCTGCCACC AATGTTGTTC AGGCACTGAG TCAGCACGTA 
GATAATTACT TACTTCTACA GCCATTTTGC ACAAAAGCCC AGTTTTTAAA TGGAAAGGCA 
AAACAGGATA TGACTGAAAA GCTTGCTGAT ATTGTTATGG AACTTTATCA AAGGAAGCCC 
CATGCAACAG AACAGAAAGT GTTGGTTGTT TTATGGCATC TCTTAGGGAA CATGACAAAT 
AGTGGCTCTT TGCCTGGAGC TGGAGGAAAT ATACGAACAG CCACAGCTAA ATTATCAAAA 
GCACTTTTTA CACAGATGGG TCAGAATCTG TTAAATCAGG CTGCATCTCA ACCATCACAT 
ATAAAAAAGA GTTTAGAAGA ATTGCTTGAT ATGACAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_010817290.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815592.1 Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X5, mRNA.

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CloneID	OBa54596
Clone ID Related Accession (Same CDS sequence)	XM_010817290.2 , XM_010824927.2
Accession Version	XM_010817290.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein FAM179B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817290.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAATATGT TTCTTTGTTC TAGATGAAGC ATCTCCTGGA 
GCCATTCCAC AGTATAAACA AAGGATGCCC TCTGTCACTC ATAGTCCAGA AATAATGGAT 
TCATCAGAAC TACGGCCATT TTCCAAACCC GAAGTAGCAC TGACAGAAGC TCTGAGGCTT 
TTAGCTGATG AGGATTGGGA GAAGAAAATT GAGGGACTGA ACTTTATTAG ATGTTTAGCT 
GCTTTTCATT CTGAAATACT GAACACAAAG TTGCATGAAA CAAATTTTGC AGTAGTTCAA 
GAGGTAAAAA ATTTACGCTC TGGAGTTTCT CGTGCTGCTG TGGTCTGTTT AAGTGATCTT 
TTTACTCACC TGAAAAAGAG CATGGATCAA GAACTAGATA ATACCGTAAA AGTTTTATTG 
CAGAAGTCAG GAGAATCAAA TACATTTATA AGAGAAGATG TTGACAAGGC ATTAAGAGCT 
ATGATCACTA ATGTAACTCC CGCACGTGCG GTTGTTTCTC TTATCAACGG AGGACAAAGC 
CACTTACACA TTGCAGTAAG AAGATGTGCT GCCCAGCATT TATCAGATGT GGTAGAATTT 
ATGGAACCAG AGCGTGTTTT ATCTGGAACA AAGGATATGG CTGACCGTAT ATTACCCGCT 
GCTGCTAAAT TTGCTCAGGA CAGCTCACCA GAAACCAGGT ATTATGGCCG AAAGATGCTT 
TTCCTCATGA TGTGTCATCC TAACTTTGAT AAAATGCTTG AAAAGTACGT GCCATCTAAA 
GATTTGCCAT ATATTAAGGA ATCTGTTAAA AGCTTACGGC AAAAGGGTTT GGGAGAGATA 
CCGCTAGATA CTCCTTCAGC AAAAGGAAGA CGATCTCATA CCGGCAGTGT TGGAAACACA 
AGGTCGTCAT CTGTTTCTAG GGATGCTTTC AATTCAGCTG AAAGAGAGGT AACTGAAATT 
CGTGAAATCA CCAGAAAATC AGTTCCCCGA AATTCCTTAG AAAGTGCTGA GTACATTAAA 
GTCATAACAG GTTTATTAAA TGCAAAAGAT TTTCGTGATC GTATTAATGG GATTAAGCAG 
CTTTTATCAG ATACAGAAAA CAATCAAGAG CTTGTTGTTG GAAACATTGT GAAGATAATT 
ACTTACTTCT ACAGCCATTT TGCACAAAAG CCCAGTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815592.1
CDS	180..5141
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAGTSGTHQTNLSGKCGQLGFSQICGKTGSVDSDLQYLGTTNSHQDKVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHTSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTGTTTSSVSIIGHRMNYGFGCGDFEEDRSHDISPSALKIQNKEQPRHYKHTKASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQICFFVLDEASPGAIPQYKQRMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIITYFYSHFAQKPSF

Target ORF information:

RefSeq Version	XM_010817290.2
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817290.2

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAATATGT TTCTTTGTTC TAGATGAAGC ATCTCCTGGA 
GCCATTCCAC AGTATAAACA AAGGATGCCC TCTGTCACTC ATAGTCCAGA AATAATGGAT 
TCATCAGAAC TACGGCCATT TTCCAAACCC GAAGTAGCAC TGACAGAAGC TCTGAGGCTT 
TTAGCTGATG AGGATTGGGA GAAGAAAATT GAGGGACTGA ACTTTATTAG ATGTTTAGCT 
GCTTTTCATT CTGAAATACT GAACACAAAG TTGCATGAAA CAAATTTTGC AGTAGTTCAA 
GAGGTAAAAA ATTTACGCTC TGGAGTTTCT CGTGCTGCTG TGGTCTGTTT AAGTGATCTT 
TTTACTCACC TGAAAAAGAG CATGGATCAA GAACTAGATA ATACCGTAAA AGTTTTATTG 
CAGAAGTCAG GAGAATCAAA TACATTTATA AGAGAAGATG TTGACAAGGC ATTAAGAGCT 
ATGATCACTA ATGTAACTCC CGCACGTGCG GTTGTTTCTC TTATCAACGG AGGACAAAGC 
CACTTACACA TTGCAGTAAG AAGATGTGCT GCCCAGCATT TATCAGATGT GGTAGAATTT 
ATGGAACCAG AGCGTGTTTT ATCTGGAACA AAGGATATGG CTGACCGTAT ATTACCCGCT 
GCTGCTAAAT TTGCTCAGGA CAGCTCACCA GAAACCAGGT ATTATGGCCG AAAGATGCTT 
TTCCTCATGA TGTGTCATCC TAACTTTGAT AAAATGCTTG AAAAGTACGT GCCATCTAAA 
GATTTGCCAT ATATTAAGGA ATCTGTTAAA AGCTTACGGC AAAAGGGTTT GGGAGAGATA 
CCGCTAGATA CTCCTTCAGC AAAAGGAAGA CGATCTCATA CCGGCAGTGT TGGAAACACA 
AGGTCGTCAT CTGTTTCTAG GGATGCTTTC AATTCAGCTG AAAGAGAGGT AACTGAAATT 
CGTGAAATCA CCAGAAAATC AGTTCCCCGA AATTCCTTAG AAAGTGCTGA GTACATTAAA 
GTCATAACAG GTTTATTAAA TGCAAAAGAT TTTCGTGATC GTATTAATGG GATTAAGCAG 
CTTTTATCAG ATACAGAAAA CAATCAAGAG CTTGTTGTTG GAAACATTGT GAAGATAATT 
ACTTACTTCT ACAGCCATTT TGCACAAAAG CCCAGTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOGARAM1 ( XM_010824927.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823229.1 Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X5, mRNA.

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CloneID	OBa54596
Clone ID Related Accession (Same CDS sequence)	XM_010817290.2 , XM_010824927.2
Accession Version	XM_010824927.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein FAM179B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646539.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824927.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAATATGT TTCTTTGTTC TAGATGAAGC ATCTCCTGGA 
GCCATTCCAC AGTATAAACA AAGGATGCCC TCTGTCACTC ATAGTCCAGA AATAATGGAT 
TCATCAGAAC TACGGCCATT TTCCAAACCC GAAGTAGCAC TGACAGAAGC TCTGAGGCTT 
TTAGCTGATG AGGATTGGGA GAAGAAAATT GAGGGACTGA ACTTTATTAG ATGTTTAGCT 
GCTTTTCATT CTGAAATACT GAACACAAAG TTGCATGAAA CAAATTTTGC AGTAGTTCAA 
GAGGTAAAAA ATTTACGCTC TGGAGTTTCT CGTGCTGCTG TGGTCTGTTT AAGTGATCTT 
TTTACTCACC TGAAAAAGAG CATGGATCAA GAACTAGATA ATACCGTAAA AGTTTTATTG 
CAGAAGTCAG GAGAATCAAA TACATTTATA AGAGAAGATG TTGACAAGGC ATTAAGAGCT 
ATGATCACTA ATGTAACTCC CGCACGTGCG GTTGTTTCTC TTATCAACGG AGGACAAAGC 
CACTTACACA TTGCAGTAAG AAGATGTGCT GCCCAGCATT TATCAGATGT GGTAGAATTT 
ATGGAACCAG AGCGTGTTTT ATCTGGAACA AAGGATATGG CTGACCGTAT ATTACCCGCT 
GCTGCTAAAT TTGCTCAGGA CAGCTCACCA GAAACCAGGT ATTATGGCCG AAAGATGCTT 
TTCCTCATGA TGTGTCATCC TAACTTTGAT AAAATGCTTG AAAAGTACGT GCCATCTAAA 
GATTTGCCAT ATATTAAGGA ATCTGTTAAA AGCTTACGGC AAAAGGGTTT GGGAGAGATA 
CCGCTAGATA CTCCTTCAGC AAAAGGAAGA CGATCTCATA CCGGCAGTGT TGGAAACACA 
AGGTCGTCAT CTGTTTCTAG GGATGCTTTC AATTCAGCTG AAAGAGAGGT AACTGAAATT 
CGTGAAATCA CCAGAAAATC AGTTCCCCGA AATTCCTTAG AAAGTGCTGA GTACATTAAA 
GTCATAACAG GTTTATTAAA TGCAAAAGAT TTTCGTGATC GTATTAATGG GATTAAGCAG 
CTTTTATCAG ATACAGAAAA CAATCAAGAG CTTGTTGTTG GAAACATTGT GAAGATAATT 
ACTTACTTCT ACAGCCATTT TGCACAAAAG CCCAGTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823229.1
CDS	180..5141
Translation	MAAAPSALLPLPPLPSLCAYRLQSRSRPSAPETDDSRGGGAMRGEKNYCCRGAAGDHGSCPPTPSPLASTLLMPAEAISSSWSGPGGGLSGGDEEETRLLQQLRTAPDPSEAFQALQAALPRRGGRLGFPRRKEALYRALGRVLVEGGSDEKRLCLQFLSDVLRGQGELGQLEEAFSLALLPQLVVSLREENPALRKDALQILHICLKRSSGQVLRTLIQQGLESTDARLRASTALLFPILLTPEDLLLDLDLTEVIISLARKLGGQEIEEESETAFSALQQIGERLGQERFQSYISRLPSALRRHYNRRLESQFGSQVPYYLELEASGFPEDPLPCAGSLSNSNLKFGIIPQELHSRLLDQEDYKNRTQAVEELKQVMGRFNPSSTSHPSLVSFISLLYNLLDDSNFKVVHGTLQVLHLLVIRLGEQVQQFLGPVIAASVKVLADNKLVIKQEYMKIFLKLMKEVGPQQVLCLLLEHLKHKHSRVREEVVNICICSLLTYPSEDFDLSKLSFDLAPALVDSKRRVRQAALEAFAVLASSMGSGKTSILFKAVDTVELQDNGDGVMNAVQARLARKTLPKLTEQGFVEYAVLIPSSAQGRSSHLAHGADADWLLAGNRTQSAHCHCGDHTRDSMQTYGSYSPTICTRRVLSAGKGKNKLPWENEQPGVTGENHTSSSKDIEQFSAYDFIPSPKLKPSQGMPVNDDLCFSRKRASRTVFQNSREFNPDSLPICSAGTSGTHQTNLSGKCGQLGFSQICGKTGSVDSDLQYLGTTNSHQDKVCASLCFGSKTQQTFGSQTERSSYSGPNSSSGAVLLPSYPLSSPRTSPKHTSLTVSPKKSQDNSVNFSSSWPLKSFEGLPKPSPQKKLVSQKSSDPTGENSQEKPPQVQLTPALVRSPSSRRGLNGTRPVPPIPRGLSLLPDKAGLSTVGPKKKEPDDIWKSEKDNLTVDLSELNIRDKDLDQEEMQSSLRSLRNSAAKKRAKLSGSTSDLESPDSAMKLDLTVDSPSRSSSPNTSSYSESGVYSQESLTSSLSTTPQGKRIMSDIFPTFGSKPCPTRLSSAKNKNSQVAEHSPSTGTTTSSVSIIGHRMNYGFGCGDFEEDRSHDISPSALKIQNKEQPRHYKHTKASTGSSSNFHQISSFDFMSTNTLSEDSVVVVGKGVFGNPIPAPATCSQSVITSVENGVSFSVKNTEPPSGIYGRAVQQSIPSYVDSENEKDIKVSISKSTYDKMRQKRKEEKELFDIKDSERKEQNPWERMKYTGNEKMAPQICFFVLDEASPGAIPQYKQRMPSVTHSPEIMDSSELRPFSKPEVALTEALRLLADEDWEKKIEGLNFIRCLAAFHSEILNTKLHETNFAVVQEVKNLRSGVSRAAVVCLSDLFTHLKKSMDQELDNTVKVLLQKSGESNTFIREDVDKALRAMITNVTPARAVVSLINGGQSHLHIAVRRCAAQHLSDVVEFMEPERVLSGTKDMADRILPAAAKFAQDSSPETRYYGRKMLFLMMCHPNFDKMLEKYVPSKDLPYIKESVKSLRQKGLGEIPLDTPSAKGRRSHTGSVGNTRSSSVSRDAFNSAEREVTEIREITRKSVPRNSLESAEYIKVITGLLNAKDFRDRINGIKQLLSDTENNQELVVGNIVKIITYFYSHFAQKPSF

Target ORF information:

RefSeq Version	XM_010824927.2
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 179 member B (FAM179B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824927.2

ORF Insert Sequence:

ATGGCGGCTG CGCCCTCAGC CCTGCTCCCG CTGCCGCCCC TTCCATCCCT CTGTGCCTAT 
CGCCTCCAGA GCCGCAGTCG TCCTTCCGCC CCAGAGACTG ATGATAGTCG AGGTGGGGGC 
GCCATGAGAG GCGAGAAAAA CTATTGCTGC CGTGGAGCTG CCGGAGATCA CGGTTCCTGC 
CCCCCGACAC CTTCGCCTCT GGCCTCGACC CTCTTGATGC CCGCGGAGGC AATCTCAAGT 
AGTTGGTCTG GGCCTGGAGG TGGTTTGTCG GGGGGAGATG AAGAGGAGAC TCGGCTCCTT 
CAACAACTGC GGACCGCGCC CGATCCTTCC GAGGCCTTCC AGGCTTTACA GGCTGCTTTG 
CCGCGGCGGG GCGGTCGACT TGGCTTCCCC CGACGAAAGG AAGCGCTTTA TCGGGCTCTG 
GGCCGAGTGC TTGTGGAAGG AGGTAGTGAT GAGAAACGAC TCTGCTTGCA GTTCCTCTCG 
GACGTTCTCC GGGGTCAGGG GGAGTTAGGC CAGCTGGAAG AGGCCTTTAG CTTAGCTCTT 
CTGCCTCAGC TTGTCGTCTC GCTACGAGAA GAGAATCCAG CCCTGCGGAA GGATGCGCTG 
CAGATCCTAC ACATCTGTTT GAAGCGTAGC TCTGGACAGG TGCTGAGGAC GCTTATACAA 
CAAGGACTGG AAAGTACCGA TGCCCGGCTT AGAGCTTCCA CAGCACTACT GTTCCCTATC 
TTGCTTACCC CTGAGGATTT GTTGCTCGAC CTAGATCTTA CCGAAGTGAT AATATCCTTA 
GCCCGAAAGC TTGGCGGTCA GGAGATAGAA GAAGAATCTG AGACAGCTTT CTCTGCACTT 
CAACAAATAG GGGAGCGTCT TGGCCAAGAG CGATTTCAGT CCTATATCTC TCGCCTGCCT 
TCTGCCTTGA GGAGACACTA CAATCGCCGC CTGGAGTCCC AGTTTGGAAG TCAGGTTCCT 
TATTATTTGG AACTTGAAGC ATCTGGATTT CCTGAAGATC CCCTTCCCTG TGCAGGGAGT 
CTTTCCAACA GCAATCTTAA ATTTGGGATT ATTCCTCAGG AGCTGCATTC AAGGTTGTTA 
GATCAGGAAG ACTATAAGAA CCGGACTCAG GCTGTTGAGG AACTGAAACA GGTGATGGGT 
CGCTTTAACC CAAGTTCCAC CTCTCATCCT AGCCTTGTGA GTTTCATTAG TTTGCTGTAT 
AATTTGTTAG ACGATTCTAA CTTCAAAGTG GTCCATGGCA CACTTCAAGT CCTGCATTTA 
CTGGTTATTC GCCTTGGAGA GCAGGTACAA CAGTTCTTGG GACCTGTTAT AGCAGCTTCT 
GTCAAAGTGC TGGCAGACAA CAAGTTGGTG ATCAAACAAG AGTACATGAA AATCTTCCTC 
AAGCTAATGA AGGAAGTAGG TCCTCAACAG GTGCTTTGCT TGCTCCTGGA ACATCTCAAA 
CATAAGCATT CCAGAGTGAG AGAGGAGGTG GTGAACATTT GCATCTGCTC CCTCCTGACT 
TATCCCAGTG AGGATTTTGA CTTATCCAAA CTGTCTTTTG ATCTTGCCCC AGCTCTTGTA 
GATAGCAAAC GCAGGGTACG CCAGGCAGCT CTTGAAGCCT TTGCTGTGTT GGCATCGTCA 
ATGGGCTCAG GTAAAACCAG CATCCTTTTC AAAGCTGTGG ATACTGTGGA GCTGCAGGAT 
AATGGAGATG GAGTGATGAA TGCTGTGCAG GCCAGATTGG CTAGGAAAAC CCTCCCAAAG 
TTAACAGAAC AGGGATTTGT GGAATATGCG GTACTCATTC CATCATCTGC CCAGGGTAGA 
TCGAGCCATT TGGCACATGG AGCAGATGCA GACTGGCTTT TGGCTGGTAA CAGAACTCAG 
AGTGCACACT GTCACTGTGG TGACCACACA AGGGATAGCA TGCAAACTTA TGGATCTTAT 
AGTCCAACTA TCTGTACTCG AAGGGTACTA AGTGCAGGAA AAGGAAAAAA TAAATTACCA 
TGGGAAAATG AGCAGCCTGG AGTCACGGGC GAAAACCATA CTTCCAGTTC CAAGGACATA 
GAACAGTTTT CAGCATATGA TTTCATTCCA TCTCCAAAAT TAAAGCCATC TCAAGGAATG 
CCAGTCAACG ATGATTTATG TTTTAGCAGA AAAAGAGCGT CAAGAACCGT ATTTCAGAAT 
AGTCGGGAAT TTAACCCAGA TTCTCTTCCT ATATGTTCTG CTGGCACTAG TGGGACTCAT 
CAGACAAATC TCTCTGGGAA ATGTGGACAA CTTGGATTTT CACAGATATG TGGTAAAACT 
GGCAGTGTGG ATTCTGACTT ACAATACCTG GGGACAACTA ACAGTCATCA AGATAAAGTT 
TGTGCTAGCC TATGTTTTGG TAGTAAGACA CAGCAGACAT TTGGTAGTCA AACAGAGCGT 
TCGTCATACT CTGGTCCAAA TTCAAGTTCA GGAGCTGTTC TTCTTCCATC CTATCCTCTC 
TCGTCACCTC GAACTAGTCC AAAGCACACG TCTCTTACTG TGTCTCCAAA GAAGTCTCAA 
GATAATTCTG TTAATTTCTC AAGTTCCTGG CCTCTTAAAA GCTTTGAAGG GCTTCCAAAG 
CCAAGTCCAC AGAAAAAGCT TGTCAGCCAA AAATCATCTG ATCCTACAGG AGAAAATTCT 
CAAGAGAAAC CTCCTCAAGT TCAACTTACT CCTGCTTTGG TGAGATCACC GTCTTCCCGA 
CGAGGCTTAA ATGGGACCAG ACCTGTACCT CCCATACCAC GGGGACTCAG CCTTCTGCCT 
GATAAAGCTG GTCTAAGCAC AGTGGGACCC AAAAAGAAAG AGCCTGATGA CATTTGGAAG 
AGTGAAAAAG ATAATCTTAC AGTTGATCTT TCAGAATTAA ATATCAGGGA TAAAGATTTG 
GATCAAGAAG AGATGCAGAG CTCTCTTAGG TCCCTTCGTA ATAGCGCAGC TAAGAAAAGA 
GCAAAACTGA GTGGCAGTAC TTCTGATCTT GAAAGCCCAG ATTCCGCCAT GAAGCTTGAC 
TTGACTGTGG ATTCCCCATC TCGATCTTCC TCTCCAAATA CCAGTTCCTA CAGTGAAAGT 
GGAGTTTACA GCCAAGAATC CTTGACTTCT TCTCTGTCGA CAACTCCCCA GGGGAAGAGA 
ATAATGTCAG ACATATTTCC AACTTTTGGA TCAAAACCTT GTCCAACAAG ACTTTCTTCA 
GCGAAGAATA AGAACTCTCA GGTAGCTGAA CACAGCCCCA GTACAGGGAC TACAACATCC 
AGTGTAAGCA TAATTGGTCA TCGTATGAAC TATGGGTTTG GATGTGGTGA CTTTGAAGAG 
GATAGGTCAC ATGACATTTC ACCTAGTGCT TTAAAAATAC AAAATAAGGA ACAACCAAGA 
CATTATAAGC ATACGAAAGC ATCTACAGGG TCATCATCAA ATTTCCACCA AATTTCCAGT 
TTTGACTTCA TGTCTACAAA TACCTTATCA GAAGACTCAG TAGTAGTTGT TGGAAAAGGG 
GTATTTGGAA ATCCAATTCC AGCACCAGCA ACCTGCAGTC AATCAGTGAT AACTTCTGTG 
GAAAATGGGG TTTCGTTTTC AGTTAAAAAT ACTGAACCAC CATCAGGCAT TTATGGAAGA 
GCAGTTCAGC AGAGTATTCC ATCATATGTT GATTCTGAGA ATGAAAAAGA TATTAAAGTT 
TCTATTTCAA AATCTACTTA TGACAAGATG AGACAAAAGA GAAAAGAGGA AAAAGAACTT 
TTTGACATTA AAGATTCTGA AAGGAAGGAA CAGAATCCCT GGGAACGAAT GAAATATACA 
GGAAATGAAA AAATGGCACC TCAAATATGT TTCTTTGTTC TAGATGAAGC ATCTCCTGGA 
GCCATTCCAC AGTATAAACA AAGGATGCCC TCTGTCACTC ATAGTCCAGA AATAATGGAT 
TCATCAGAAC TACGGCCATT TTCCAAACCC GAAGTAGCAC TGACAGAAGC TCTGAGGCTT 
TTAGCTGATG AGGATTGGGA GAAGAAAATT GAGGGACTGA ACTTTATTAG ATGTTTAGCT 
GCTTTTCATT CTGAAATACT GAACACAAAG TTGCATGAAA CAAATTTTGC AGTAGTTCAA 
GAGGTAAAAA ATTTACGCTC TGGAGTTTCT CGTGCTGCTG TGGTCTGTTT AAGTGATCTT 
TTTACTCACC TGAAAAAGAG CATGGATCAA GAACTAGATA ATACCGTAAA AGTTTTATTG 
CAGAAGTCAG GAGAATCAAA TACATTTATA AGAGAAGATG TTGACAAGGC ATTAAGAGCT 
ATGATCACTA ATGTAACTCC CGCACGTGCG GTTGTTTCTC TTATCAACGG AGGACAAAGC 
CACTTACACA TTGCAGTAAG AAGATGTGCT GCCCAGCATT TATCAGATGT GGTAGAATTT 
ATGGAACCAG AGCGTGTTTT ATCTGGAACA AAGGATATGG CTGACCGTAT ATTACCCGCT 
GCTGCTAAAT TTGCTCAGGA CAGCTCACCA GAAACCAGGT ATTATGGCCG AAAGATGCTT 
TTCCTCATGA TGTGTCATCC TAACTTTGAT AAAATGCTTG AAAAGTACGT GCCATCTAAA 
GATTTGCCAT ATATTAAGGA ATCTGTTAAA AGCTTACGGC AAAAGGGTTT GGGAGAGATA 
CCGCTAGATA CTCCTTCAGC AAAAGGAAGA CGATCTCATA CCGGCAGTGT TGGAAACACA 
AGGTCGTCAT CTGTTTCTAG GGATGCTTTC AATTCAGCTG AAAGAGAGGT AACTGAAATT 
CGTGAAATCA CCAGAAAATC AGTTCCCCGA AATTCCTTAG AAAGTGCTGA GTACATTAAA 
GTCATAACAG GTTTATTAAA TGCAAAAGAT TTTCGTGATC GTATTAATGG GATTAAGCAG 
CTTTTATCAG ATACAGAAAA CAATCAAGAG CTTGTTGTTG GAAACATTGT GAAGATAATT 
ACTTACTTCT ACAGCCATTT TGCACAAAAG CCCAGTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TOGARAM1
Entrez Gene ID	540222
Full Name	TOG array regulator of axonemal microtubules 1
Synonyms	FAM179B
General protein information	Preferred Names TOG array regulator of axonemal microtubules 1 Names protein FAM179B family with sequence similarity 179 member B
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	FAM179B	NP_055906.2
Mus musculus (house mouse)	Fam179b	NP_808473.2
Xenopus tropicalis (tropical clawed frog)	LOC100495084	XP_002940589.2
Pan troglodytes (chimpanzee)	FAM179B	XP_001150036.1
	LOC702188	XP_001095789.1
Canis lupus familiaris (dog)	FAM179B	XP_547791.3
Bos taurus (cattle)	FAM179B	XP_005222318.1
Rattus norvegicus (Norway rat)	Fam179b	XP_234236.4
Gallus gallus (chicken)	FAM179B	XP_421483.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TOGARAM1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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