RFWD3 cDNA ORF clone, Bos taurus(cattle)

The following RFWD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFWD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa09182 XM_002694723.6
Latest version!
Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09182 XM_591339.8
Latest version!
Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09183 XM_005195170.3
Latest version!
Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09183 XM_005218380.4
Latest version!
Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
OBa09182 XM_002694723.5 Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa09183 XM_005218380.3 Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa09182
    Clone ID Related Accession (Same CDS sequence) XM_002694723.6 , XM_002694723.5 , XM_591339.8
    Accession Version XM_002694723.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2373bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase RFWD3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002694723.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
    CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT
    CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG
    GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC
    AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA
    GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT
    AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC
    TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC
    CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA
    AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC
    TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG
    AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC
    ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG
    GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC
    CCCAAACAGC AGTCTCCCCA GAAGTCTAAC CCTTCTCTAA CTTCTGCTTC TGTGGATGAG
    GAAGAAGGAG ACACTTGCAC AATATGTTTG GAGCAGTGGA CCAATGCTGG GGATCACCGG
    CTCTCTACAT TGCGCTGTGG GCACCTCTTT GGGTACAAAT GCATTTCGAA ATGGCTCAGA
    GGACAGACCC GAAAATGTCC CCAGTGTAAC AAGAAAGCCA AGCAAAGTGA TATCGTTGTC
    CTTTATGCCC GGACCTTGAG AGCTTTGGAC ACTAGTGAGC AGGAGCTCAT GAAGAGTTCC
    TTACTAAAGG AACAGAAGCT AAGGAAGAAG GCTGAATTAG ACTCGGCACA GTGTCGGCTG
    CAGCTTCAAG CCCTCACTGA TAAATGCTCT AGGCTTCAAA GTCGTGTTCA GGACTTGCAG
    AAACTTATCG GTCAGGATAA GATCTCACAA CAGCCTGGGG GTTCCCAACC ACATTTCCTA
    AGTGGCCTGC CCACCAGCCA GAGTCAACAG AAGTACCATT TCCAGAAGAC ATTCACAGTG
    TCTCAAACAG GAAACTGTCG AGTCATGACA TACTGTGATG CTTTGAGCTG CCTGGTGGTA
    TCACAACCTT CTCCTCAGTC CGCCTTCCTT CCAGGCTTTG GTGTTAAGAT GTTGAGTACT
    GCCAACATGA AAAGCAGCCA GTATATTCCT ATGCATGGCA AACAAATACG TGGACTGGCT
    TTCAGCAGTC GATCCAAAGG CTTACTTCTC TCTGCTTCCC TGGACAGCAC AATTAAACTG
    ACCAGCCTGG AAACAAATAC CGTGGTCCAG ACTTACAACG CTGGGCGGCC TGTCTGGAGC
    TGCTGCTGGT GTCTTGATGA AAACAATTAC ATCTATGCTG GACTGGTCAA CGGTTCGATT
    CTGTTATATG ACCTTCGAAA TACAAGCTCT CACGTCCAGG AATTAGTACC TCAGAGAGCT
    AGATGCCCGC TGGTATCCCT GTCGTATGTG CCCAGAGCTG CCTCTGCTGC GTTTCCATAT
    GGTGGCGTGT TGGCTGGAGC GTTGGAGAAT GCCTCTTTTT GGGAGCTGAA AATGGGTTTT
    ACTCATTGGC CTCATGTGCT GCCCATGGAG CCTGGGGGCT GCGTAGACTT TCAGGTAGAG
    AGCAGCACCC GCCACTGTCT CGTGACCTAT AGGCCTGATA AAAATCACAA CAACTTACGA
    AGTGTACTCA TGGAGATGTC CTATAAACTG GATAATGCTG GAGAGCCCAT CTGCTCCTGC
    CATCCTGTAC AAACATTCTT TGGGGGACCC TCCTGCAAAT TGCTAACCAA GAATGCCATT
    TTCCAAAATC CAGAGAATAA TGGCAGCATC CTGGTGTGTA TTGGGGATGA AGCATCAAAA
    TCTGCCCTGC TGTGGGATGC TGGCAGTGGC TCATTGCTGC AGGATCTGCA GGCCGATCAG
    CCTGTCTTGG ACATCTGCCC ATTTGACGTG AATCATAACA GCTACTTGGC TACCTTAACA
    GAAAAGACCG TCCACGTCTA TAGGTGGGAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694769.1
    CDS157..2529
    Translation

    Target ORF information:

    RefSeq Version XM_002694723.6
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694723.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
    CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT
    CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG
    GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC
    AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA
    GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT
    AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC
    TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC
    CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA
    AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC
    TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG
    AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC
    ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG
    GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC
    CCCAAACAGC AGTCTCCCCA GAAGTCTAAC CCTTCTCTAA CTTCTGCTTC TGTGGATGAG
    GAAGAAGGAG ACACTTGCAC AATATGTTTG GAGCAGTGGA CCAATGCTGG GGATCACCGG
    CTCTCTACAT TGCGCTGTGG GCACCTCTTT GGGTACAAAT GCATTTCGAA ATGGCTCAGA
    GGACAGACCC GAAAATGTCC CCAGTGTAAC AAGAAAGCCA AGCAAAGTGA TATCGTTGTC
    CTTTATGCCC GGACCTTGAG AGCTTTGGAC ACTAGTGAGC AGGAGCTCAT GAAGAGTTCC
    TTACTAAAGG AACAGAAGCT AAGGAAGAAG GCTGAATTAG ACTCGGCACA GTGTCGGCTG
    CAGCTTCAAG CCCTCACTGA TAAATGCTCT AGGCTTCAAA GTCGTGTTCA GGACTTGCAG
    AAACTTATCG GTCAGGATAA GATCTCACAA CAGCCTGGGG GTTCCCAACC ACATTTCCTA
    AGTGGCCTGC CCACCAGCCA GAGTCAACAG AAGTACCATT TCCAGAAGAC ATTCACAGTG
    TCTCAAACAG GAAACTGTCG AGTCATGACA TACTGTGATG CTTTGAGCTG CCTGGTGGTA
    TCACAACCTT CTCCTCAGTC CGCCTTCCTT CCAGGCTTTG GTGTTAAGAT GTTGAGTACT
    GCCAACATGA AAAGCAGCCA GTATATTCCT ATGCATGGCA AACAAATACG TGGACTGGCT
    TTCAGCAGTC GATCCAAAGG CTTACTTCTC TCTGCTTCCC TGGACAGCAC AATTAAACTG
    ACCAGCCTGG AAACAAATAC CGTGGTCCAG ACTTACAACG CTGGGCGGCC TGTCTGGAGC
    TGCTGCTGGT GTCTTGATGA AAACAATTAC ATCTATGCTG GACTGGTCAA CGGTTCGATT
    CTGTTATATG ACCTTCGAAA TACAAGCTCT CACGTCCAGG AATTAGTACC TCAGAGAGCT
    AGATGCCCGC TGGTATCCCT GTCGTATGTG CCCAGAGCTG CCTCTGCTGC GTTTCCATAT
    GGTGGCGTGT TGGCTGGAGC GTTGGAGAAT GCCTCTTTTT GGGAGCTGAA AATGGGTTTT
    ACTCATTGGC CTCATGTGCT GCCCATGGAG CCTGGGGGCT GCGTAGACTT TCAGGTAGAG
    AGCAGCACCC GCCACTGTCT CGTGACCTAT AGGCCTGATA AAAATCACAA CAACTTACGA
    AGTGTACTCA TGGAGATGTC CTATAAACTG GATAATGCTG GAGAGCCCAT CTGCTCCTGC
    CATCCTGTAC AAACATTCTT TGGGGGACCC TCCTGCAAAT TGCTAACCAA GAATGCCATT
    TTCCAAAATC CAGAGAATAA TGGCAGCATC CTGGTGTGTA TTGGGGATGA AGCATCAAAA
    TCTGCCCTGC TGTGGGATGC TGGCAGTGGC TCATTGCTGC AGGATCTGCA GGCCGATCAG
    CCTGTCTTGG ACATCTGCCC ATTTGACGTG AATCATAACA GCTACTTGGC TACCTTAACA
    GAAAAGACCG TCCACGTCTA TAGGTGGGAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09182
    Clone ID Related Accession (Same CDS sequence) XM_002694723.6 , XM_002694723.5 , XM_591339.8
    Accession Version XM_002694723.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2373bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase RFWD3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104462.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002694723.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
    CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT
    CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG
    GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC
    AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA
    GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT
    AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC
    TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC
    CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA
    AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC
    TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG
    AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC
    ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG
    GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC
    CCCAAACAGC AGTCTCCCCA GAAGTCTAAC CCTTCTCTAA CTTCTGCTTC TGTGGATGAG
    GAAGAAGGAG ACACTTGCAC AATATGTTTG GAGCAGTGGA CCAATGCTGG GGATCACCGG
    CTCTCTACAT TGCGCTGTGG GCACCTCTTT GGGTACAAAT GCATTTCGAA ATGGCTCAGA
    GGACAGACCC GAAAATGTCC CCAGTGTAAC AAGAAAGCCA AGCAAAGTGA TATCGTTGTC
    CTTTATGCCC GGACCTTGAG AGCTTTGGAC ACTAGTGAGC AGGAGCTCAT GAAGAGTTCC
    TTACTAAAGG AACAGAAGCT AAGGAAGAAG GCTGAATTAG ACTCGGCACA GTGTCGGCTG
    CAGCTTCAAG CCCTCACTGA TAAATGCTCT AGGCTTCAAA GTCGTGTTCA GGACTTGCAG
    AAACTTATCG GTCAGGATAA GATCTCACAA CAGCCTGGGG GTTCCCAACC ACATTTCCTA
    AGTGGCCTGC CCACCAGCCA GAGTCAACAG AAGTACCATT TCCAGAAGAC ATTCACAGTG
    TCTCAAACAG GAAACTGTCG AGTCATGACA TACTGTGATG CTTTGAGCTG CCTGGTGGTA
    TCACAACCTT CTCCTCAGTC CGCCTTCCTT CCAGGCTTTG GTGTTAAGAT GTTGAGTACT
    GCCAACATGA AAAGCAGCCA GTATATTCCT ATGCATGGCA AACAAATACG TGGACTGGCT
    TTCAGCAGTC GATCCAAAGG CTTACTTCTC TCTGCTTCCC TGGACAGCAC AATTAAACTG
    ACCAGCCTGG AAACAAATAC CGTGGTCCAG ACTTACAACG CTGGGCGGCC TGTCTGGAGC
    TGCTGCTGGT GTCTTGATGA AAACAATTAC ATCTATGCTG GACTGGTCAA CGGTTCGATT
    CTGTTATATG ACCTTCGAAA TACAAGCTCT CACGTCCAGG AATTAGTACC TCAGAGAGCT
    AGATGCCCGC TGGTATCCCT GTCGTATGTG CCCAGAGCTG CCTCTGCTGC GTTTCCATAT
    GGTGGCGTGT TGGCTGGAGC GTTGGAGAAT GCCTCTTTTT GGGAGCTGAA AATGGGTTTT
    ACTCATTGGC CTCATGTGCT GCCCATGGAG CCTGGGGGCT GCGTAGACTT TCAGGTAGAG
    AGCAGCACCC GCCACTGTCT CGTGACCTAT AGGCCTGATA AAAATCACAA CAACTTACGA
    AGTGTACTCA TGGAGATGTC CTATAAACTG GATAATGCTG GAGAGCCCAT CTGCTCCTGC
    CATCCTGTAC AAACATTCTT TGGGGGACCC TCCTGCAAAT TGCTAACCAA GAATGCCATT
    TTCCAAAATC CAGAGAATAA TGGCAGCATC CTGGTGTGTA TTGGGGATGA AGCATCAAAA
    TCTGCCCTGC TGTGGGATGC TGGCAGTGGC TCATTGCTGC AGGATCTGCA GGCCGATCAG
    CCTGTCTTGG ACATCTGCCC ATTTGACGTG AATCATAACA GCTACTTGGC TACCTTAACA
    GAAAAGACCG TCCACGTCTA TAGGTGGGAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002694769.1
    CDS140..2512
    Translation

    Target ORF information:

    RefSeq Version XM_002694723.5
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002694723.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
    CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT
    CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG
    GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC
    AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA
    GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT
    AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC
    TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC
    CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA
    AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC
    TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG
    AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC
    ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG
    GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC
    CCCAAACAGC AGTCTCCCCA GAAGTCTAAC CCTTCTCTAA CTTCTGCTTC TGTGGATGAG
    GAAGAAGGAG ACACTTGCAC AATATGTTTG GAGCAGTGGA CCAATGCTGG GGATCACCGG
    CTCTCTACAT TGCGCTGTGG GCACCTCTTT GGGTACAAAT GCATTTCGAA ATGGCTCAGA
    GGACAGACCC GAAAATGTCC CCAGTGTAAC AAGAAAGCCA AGCAAAGTGA TATCGTTGTC
    CTTTATGCCC GGACCTTGAG AGCTTTGGAC ACTAGTGAGC AGGAGCTCAT GAAGAGTTCC
    TTACTAAAGG AACAGAAGCT AAGGAAGAAG GCTGAATTAG ACTCGGCACA GTGTCGGCTG
    CAGCTTCAAG CCCTCACTGA TAAATGCTCT AGGCTTCAAA GTCGTGTTCA GGACTTGCAG
    AAACTTATCG GTCAGGATAA GATCTCACAA CAGCCTGGGG GTTCCCAACC ACATTTCCTA
    AGTGGCCTGC CCACCAGCCA GAGTCAACAG AAGTACCATT TCCAGAAGAC ATTCACAGTG
    TCTCAAACAG GAAACTGTCG AGTCATGACA TACTGTGATG CTTTGAGCTG CCTGGTGGTA
    TCACAACCTT CTCCTCAGTC CGCCTTCCTT CCAGGCTTTG GTGTTAAGAT GTTGAGTACT
    GCCAACATGA AAAGCAGCCA GTATATTCCT ATGCATGGCA AACAAATACG TGGACTGGCT
    TTCAGCAGTC GATCCAAAGG CTTACTTCTC TCTGCTTCCC TGGACAGCAC AATTAAACTG
    ACCAGCCTGG AAACAAATAC CGTGGTCCAG ACTTACAACG CTGGGCGGCC TGTCTGGAGC
    TGCTGCTGGT GTCTTGATGA AAACAATTAC ATCTATGCTG GACTGGTCAA CGGTTCGATT
    CTGTTATATG ACCTTCGAAA TACAAGCTCT CACGTCCAGG AATTAGTACC TCAGAGAGCT
    AGATGCCCGC TGGTATCCCT GTCGTATGTG CCCAGAGCTG CCTCTGCTGC GTTTCCATAT
    GGTGGCGTGT TGGCTGGAGC GTTGGAGAAT GCCTCTTTTT GGGAGCTGAA AATGGGTTTT
    ACTCATTGGC CTCATGTGCT GCCCATGGAG CCTGGGGGCT GCGTAGACTT TCAGGTAGAG
    AGCAGCACCC GCCACTGTCT CGTGACCTAT AGGCCTGATA AAAATCACAA CAACTTACGA
    AGTGTACTCA TGGAGATGTC CTATAAACTG GATAATGCTG GAGAGCCCAT CTGCTCCTGC
    CATCCTGTAC AAACATTCTT TGGGGGACCC TCCTGCAAAT TGCTAACCAA GAATGCCATT
    TTCCAAAATC CAGAGAATAA TGGCAGCATC CTGGTGTGTA TTGGGGATGA AGCATCAAAA
    TCTGCCCTGC TGTGGGATGC TGGCAGTGGC TCATTGCTGC AGGATCTGCA GGCCGATCAG
    CCTGTCTTGG ACATCTGCCC ATTTGACGTG AATCATAACA GCTACTTGGC TACCTTAACA
    GAAAAGACCG TCCACGTCTA TAGGTGGGAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09182
    Clone ID Related Accession (Same CDS sequence) XM_002694723.6 , XM_002694723.5 , XM_591339.8
    Accession Version XM_591339.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2373bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase RFWD3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646378.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_591339.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
    CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT
    CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG
    GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC
    AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA
    GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT
    AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC
    TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC
    CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA
    AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC
    TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG
    AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC
    ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG
    GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC
    CCCAAACAGC AGTCTCCCCA GAAGTCTAAC CCTTCTCTAA CTTCTGCTTC TGTGGATGAG
    GAAGAAGGAG ACACTTGCAC AATATGTTTG GAGCAGTGGA CCAATGCTGG GGATCACCGG
    CTCTCTACAT TGCGCTGTGG GCACCTCTTT GGGTACAAAT GCATTTCGAA ATGGCTCAGA
    GGACAGACCC GAAAATGTCC CCAGTGTAAC AAGAAAGCCA AGCAAAGTGA TATCGTTGTC
    CTTTATGCCC GGACCTTGAG AGCTTTGGAC ACTAGTGAGC AGGAGCTCAT GAAGAGTTCC
    TTACTAAAGG AACAGAAGCT AAGGAAGAAG GCTGAATTAG ACTCGGCACA GTGTCGGCTG
    CAGCTTCAAG CCCTCACTGA TAAATGCTCT AGGCTTCAAA GTCGTGTTCA GGACTTGCAG
    AAACTTATCG GTCAGGATAA GATCTCACAA CAGCCTGGGG GTTCCCAACC ACATTTCCTA
    AGTGGCCTGC CCACCAGCCA GAGTCAACAG AAGTACCATT TCCAGAAGAC ATTCACAGTG
    TCTCAAACAG GAAACTGTCG AGTCATGACA TACTGTGATG CTTTGAGCTG CCTGGTGGTA
    TCACAACCTT CTCCTCAGTC CGCCTTCCTT CCAGGCTTTG GTGTTAAGAT GTTGAGTACT
    GCCAACATGA AAAGCAGCCA GTATATTCCT ATGCATGGCA AACAAATACG TGGACTGGCT
    TTCAGCAGTC GATCCAAAGG CTTACTTCTC TCTGCTTCCC TGGACAGCAC AATTAAACTG
    ACCAGCCTGG AAACAAATAC CGTGGTCCAG ACTTACAACG CTGGGCGGCC TGTCTGGAGC
    TGCTGCTGGT GTCTTGATGA AAACAATTAC ATCTATGCTG GACTGGTCAA CGGTTCGATT
    CTGTTATATG ACCTTCGAAA TACAAGCTCT CACGTCCAGG AATTAGTACC TCAGAGAGCT
    AGATGCCCGC TGGTATCCCT GTCGTATGTG CCCAGAGCTG CCTCTGCTGC GTTTCCATAT
    GGTGGCGTGT TGGCTGGAGC GTTGGAGAAT GCCTCTTTTT GGGAGCTGAA AATGGGTTTT
    ACTCATTGGC CTCATGTGCT GCCCATGGAG CCTGGGGGCT GCGTAGACTT TCAGGTAGAG
    AGCAGCACCC GCCACTGTCT CGTGACCTAT AGGCCTGATA AAAATCACAA CAACTTACGA
    AGTGTACTCA TGGAGATGTC CTATAAACTG GATAATGCTG GAGAGCCCAT CTGCTCCTGC
    CATCCTGTAC AAACATTCTT TGGGGGACCC TCCTGCAAAT TGCTAACCAA GAATGCCATT
    TTCCAAAATC CAGAGAATAA TGGCAGCATC CTGGTGTGTA TTGGGGATGA AGCATCAAAA
    TCTGCCCTGC TGTGGGATGC TGGCAGTGGC TCATTGCTGC AGGATCTGCA GGCCGATCAG
    CCTGTCTTGG ACATCTGCCC ATTTGACGTG AATCATAACA GCTACTTGGC TACCTTAACA
    GAAAAGACCG TCCACGTCTA TAGGTGGGAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_591339.3
    CDS140..2512
    Translation

    Target ORF information:

    RefSeq Version XM_591339.8
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_591339.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
    CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT
    CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG
    GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC
    AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA
    GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT
    AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC
    TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC
    CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA
    AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC
    TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG
    AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC
    ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG
    GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC
    CCCAAACAGC AGTCTCCCCA GAAGTCTAAC CCTTCTCTAA CTTCTGCTTC TGTGGATGAG
    GAAGAAGGAG ACACTTGCAC AATATGTTTG GAGCAGTGGA CCAATGCTGG GGATCACCGG
    CTCTCTACAT TGCGCTGTGG GCACCTCTTT GGGTACAAAT GCATTTCGAA ATGGCTCAGA
    GGACAGACCC GAAAATGTCC CCAGTGTAAC AAGAAAGCCA AGCAAAGTGA TATCGTTGTC
    CTTTATGCCC GGACCTTGAG AGCTTTGGAC ACTAGTGAGC AGGAGCTCAT GAAGAGTTCC
    TTACTAAAGG AACAGAAGCT AAGGAAGAAG GCTGAATTAG ACTCGGCACA GTGTCGGCTG
    CAGCTTCAAG CCCTCACTGA TAAATGCTCT AGGCTTCAAA GTCGTGTTCA GGACTTGCAG
    AAACTTATCG GTCAGGATAA GATCTCACAA CAGCCTGGGG GTTCCCAACC ACATTTCCTA
    AGTGGCCTGC CCACCAGCCA GAGTCAACAG AAGTACCATT TCCAGAAGAC ATTCACAGTG
    TCTCAAACAG GAAACTGTCG AGTCATGACA TACTGTGATG CTTTGAGCTG CCTGGTGGTA
    TCACAACCTT CTCCTCAGTC CGCCTTCCTT CCAGGCTTTG GTGTTAAGAT GTTGAGTACT
    GCCAACATGA AAAGCAGCCA GTATATTCCT ATGCATGGCA AACAAATACG TGGACTGGCT
    TTCAGCAGTC GATCCAAAGG CTTACTTCTC TCTGCTTCCC TGGACAGCAC AATTAAACTG
    ACCAGCCTGG AAACAAATAC CGTGGTCCAG ACTTACAACG CTGGGCGGCC TGTCTGGAGC
    TGCTGCTGGT GTCTTGATGA AAACAATTAC ATCTATGCTG GACTGGTCAA CGGTTCGATT
    CTGTTATATG ACCTTCGAAA TACAAGCTCT CACGTCCAGG AATTAGTACC TCAGAGAGCT
    AGATGCCCGC TGGTATCCCT GTCGTATGTG CCCAGAGCTG CCTCTGCTGC GTTTCCATAT
    GGTGGCGTGT TGGCTGGAGC GTTGGAGAAT GCCTCTTTTT GGGAGCTGAA AATGGGTTTT
    ACTCATTGGC CTCATGTGCT GCCCATGGAG CCTGGGGGCT GCGTAGACTT TCAGGTAGAG
    AGCAGCACCC GCCACTGTCT CGTGACCTAT AGGCCTGATA AAAATCACAA CAACTTACGA
    AGTGTACTCA TGGAGATGTC CTATAAACTG GATAATGCTG GAGAGCCCAT CTGCTCCTGC
    CATCCTGTAC AAACATTCTT TGGGGGACCC TCCTGCAAAT TGCTAACCAA GAATGCCATT
    TTCCAAAATC CAGAGAATAA TGGCAGCATC CTGGTGTGTA TTGGGGATGA AGCATCAAAA
    TCTGCCCTGC TGTGGGATGC TGGCAGTGGC TCATTGCTGC AGGATCTGCA GGCCGATCAG
    CCTGTCTTGG ACATCTGCCC ATTTGACGTG AATCATAACA GCTACTTGGC TACCTTAACA
    GAAAAGACCG TCCACGTCTA TAGGTGGGAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09183
    Clone ID Related Accession (Same CDS sequence) XM_005195170.3 , XM_005218380.3 , XM_005218380.4
    Accession Version XM_005195170.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2370bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase RFWD3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646378.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195170.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
    CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT
    CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG
    GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC
    AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA
    GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT
    AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC
    TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC
    CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA
    AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC
    TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG
    AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC
    ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG
    GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC
    CCCAAACAGT CTCCCCAGAA GTCTAACCCT TCTCTAACTT CTGCTTCTGT GGATGAGGAA
    GAAGGAGACA CTTGCACAAT ATGTTTGGAG CAGTGGACCA ATGCTGGGGA TCACCGGCTC
    TCTACATTGC GCTGTGGGCA CCTCTTTGGG TACAAATGCA TTTCGAAATG GCTCAGAGGA
    CAGACCCGAA AATGTCCCCA GTGTAACAAG AAAGCCAAGC AAAGTGATAT CGTTGTCCTT
    TATGCCCGGA CCTTGAGAGC TTTGGACACT AGTGAGCAGG AGCTCATGAA GAGTTCCTTA
    CTAAAGGAAC AGAAGCTAAG GAAGAAGGCT GAATTAGACT CGGCACAGTG TCGGCTGCAG
    CTTCAAGCCC TCACTGATAA ATGCTCTAGG CTTCAAAGTC GTGTTCAGGA CTTGCAGAAA
    CTTATCGGTC AGGATAAGAT CTCACAACAG CCTGGGGGTT CCCAACCACA TTTCCTAAGT
    GGCCTGCCCA CCAGCCAGAG TCAACAGAAG TACCATTTCC AGAAGACATT CACAGTGTCT
    CAAACAGGAA ACTGTCGAGT CATGACATAC TGTGATGCTT TGAGCTGCCT GGTGGTATCA
    CAACCTTCTC CTCAGTCCGC CTTCCTTCCA GGCTTTGGTG TTAAGATGTT GAGTACTGCC
    AACATGAAAA GCAGCCAGTA TATTCCTATG CATGGCAAAC AAATACGTGG ACTGGCTTTC
    AGCAGTCGAT CCAAAGGCTT ACTTCTCTCT GCTTCCCTGG ACAGCACAAT TAAACTGACC
    AGCCTGGAAA CAAATACCGT GGTCCAGACT TACAACGCTG GGCGGCCTGT CTGGAGCTGC
    TGCTGGTGTC TTGATGAAAA CAATTACATC TATGCTGGAC TGGTCAACGG TTCGATTCTG
    TTATATGACC TTCGAAATAC AAGCTCTCAC GTCCAGGAAT TAGTACCTCA GAGAGCTAGA
    TGCCCGCTGG TATCCCTGTC GTATGTGCCC AGAGCTGCCT CTGCTGCGTT TCCATATGGT
    GGCGTGTTGG CTGGAGCGTT GGAGAATGCC TCTTTTTGGG AGCTGAAAAT GGGTTTTACT
    CATTGGCCTC ATGTGCTGCC CATGGAGCCT GGGGGCTGCG TAGACTTTCA GGTAGAGAGC
    AGCACCCGCC ACTGTCTCGT GACCTATAGG CCTGATAAAA ATCACAACAA CTTACGAAGT
    GTACTCATGG AGATGTCCTA TAAACTGGAT AATGCTGGAG AGCCCATCTG CTCCTGCCAT
    CCTGTACAAA CATTCTTTGG GGGACCCTCC TGCAAATTGC TAACCAAGAA TGCCATTTTC
    CAAAATCCAG AGAATAATGG CAGCATCCTG GTGTGTATTG GGGATGAAGC ATCAAAATCT
    GCCCTGCTGT GGGATGCTGG CAGTGGCTCA TTGCTGCAGG ATCTGCAGGC CGATCAGCCT
    GTCTTGGACA TCTGCCCATT TGACGTGAAT CATAACAGCT ACTTGGCTAC CTTAACAGAA
    AAGACCGTCC ACGTCTATAG GTGGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195227.1
    CDS140..2509
    Translation

    Target ORF information:

    RefSeq Version XM_005195170.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195170.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
    CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT
    CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG
    GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC
    AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA
    GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT
    AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC
    TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC
    CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA
    AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC
    TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG
    AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC
    ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG
    GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC
    CCCAAACAGT CTCCCCAGAA GTCTAACCCT TCTCTAACTT CTGCTTCTGT GGATGAGGAA
    GAAGGAGACA CTTGCACAAT ATGTTTGGAG CAGTGGACCA ATGCTGGGGA TCACCGGCTC
    TCTACATTGC GCTGTGGGCA CCTCTTTGGG TACAAATGCA TTTCGAAATG GCTCAGAGGA
    CAGACCCGAA AATGTCCCCA GTGTAACAAG AAAGCCAAGC AAAGTGATAT CGTTGTCCTT
    TATGCCCGGA CCTTGAGAGC TTTGGACACT AGTGAGCAGG AGCTCATGAA GAGTTCCTTA
    CTAAAGGAAC AGAAGCTAAG GAAGAAGGCT GAATTAGACT CGGCACAGTG TCGGCTGCAG
    CTTCAAGCCC TCACTGATAA ATGCTCTAGG CTTCAAAGTC GTGTTCAGGA CTTGCAGAAA
    CTTATCGGTC AGGATAAGAT CTCACAACAG CCTGGGGGTT CCCAACCACA TTTCCTAAGT
    GGCCTGCCCA CCAGCCAGAG TCAACAGAAG TACCATTTCC AGAAGACATT CACAGTGTCT
    CAAACAGGAA ACTGTCGAGT CATGACATAC TGTGATGCTT TGAGCTGCCT GGTGGTATCA
    CAACCTTCTC CTCAGTCCGC CTTCCTTCCA GGCTTTGGTG TTAAGATGTT GAGTACTGCC
    AACATGAAAA GCAGCCAGTA TATTCCTATG CATGGCAAAC AAATACGTGG ACTGGCTTTC
    AGCAGTCGAT CCAAAGGCTT ACTTCTCTCT GCTTCCCTGG ACAGCACAAT TAAACTGACC
    AGCCTGGAAA CAAATACCGT GGTCCAGACT TACAACGCTG GGCGGCCTGT CTGGAGCTGC
    TGCTGGTGTC TTGATGAAAA CAATTACATC TATGCTGGAC TGGTCAACGG TTCGATTCTG
    TTATATGACC TTCGAAATAC AAGCTCTCAC GTCCAGGAAT TAGTACCTCA GAGAGCTAGA
    TGCCCGCTGG TATCCCTGTC GTATGTGCCC AGAGCTGCCT CTGCTGCGTT TCCATATGGT
    GGCGTGTTGG CTGGAGCGTT GGAGAATGCC TCTTTTTGGG AGCTGAAAAT GGGTTTTACT
    CATTGGCCTC ATGTGCTGCC CATGGAGCCT GGGGGCTGCG TAGACTTTCA GGTAGAGAGC
    AGCACCCGCC ACTGTCTCGT GACCTATAGG CCTGATAAAA ATCACAACAA CTTACGAAGT
    GTACTCATGG AGATGTCCTA TAAACTGGAT AATGCTGGAG AGCCCATCTG CTCCTGCCAT
    CCTGTACAAA CATTCTTTGG GGGACCCTCC TGCAAATTGC TAACCAAGAA TGCCATTTTC
    CAAAATCCAG AGAATAATGG CAGCATCCTG GTGTGTATTG GGGATGAAGC ATCAAAATCT
    GCCCTGCTGT GGGATGCTGG CAGTGGCTCA TTGCTGCAGG ATCTGCAGGC CGATCAGCCT
    GTCTTGGACA TCTGCCCATT TGACGTGAAT CATAACAGCT ACTTGGCTAC CTTAACAGAA
    AAGACCGTCC ACGTCTATAG GTGGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09183
    Clone ID Related Accession (Same CDS sequence) XM_005195170.3 , XM_005218380.3 , XM_005218380.4
    Accession Version XM_005218380.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2370bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase RFWD3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104462.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218380.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
    CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT
    CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG
    GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC
    AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA
    GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT
    AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC
    TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC
    CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA
    AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC
    TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG
    AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC
    ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG
    GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC
    CCCAAACAGT CTCCCCAGAA GTCTAACCCT TCTCTAACTT CTGCTTCTGT GGATGAGGAA
    GAAGGAGACA CTTGCACAAT ATGTTTGGAG CAGTGGACCA ATGCTGGGGA TCACCGGCTC
    TCTACATTGC GCTGTGGGCA CCTCTTTGGG TACAAATGCA TTTCGAAATG GCTCAGAGGA
    CAGACCCGAA AATGTCCCCA GTGTAACAAG AAAGCCAAGC AAAGTGATAT CGTTGTCCTT
    TATGCCCGGA CCTTGAGAGC TTTGGACACT AGTGAGCAGG AGCTCATGAA GAGTTCCTTA
    CTAAAGGAAC AGAAGCTAAG GAAGAAGGCT GAATTAGACT CGGCACAGTG TCGGCTGCAG
    CTTCAAGCCC TCACTGATAA ATGCTCTAGG CTTCAAAGTC GTGTTCAGGA CTTGCAGAAA
    CTTATCGGTC AGGATAAGAT CTCACAACAG CCTGGGGGTT CCCAACCACA TTTCCTAAGT
    GGCCTGCCCA CCAGCCAGAG TCAACAGAAG TACCATTTCC AGAAGACATT CACAGTGTCT
    CAAACAGGAA ACTGTCGAGT CATGACATAC TGTGATGCTT TGAGCTGCCT GGTGGTATCA
    CAACCTTCTC CTCAGTCCGC CTTCCTTCCA GGCTTTGGTG TTAAGATGTT GAGTACTGCC
    AACATGAAAA GCAGCCAGTA TATTCCTATG CATGGCAAAC AAATACGTGG ACTGGCTTTC
    AGCAGTCGAT CCAAAGGCTT ACTTCTCTCT GCTTCCCTGG ACAGCACAAT TAAACTGACC
    AGCCTGGAAA CAAATACCGT GGTCCAGACT TACAACGCTG GGCGGCCTGT CTGGAGCTGC
    TGCTGGTGTC TTGATGAAAA CAATTACATC TATGCTGGAC TGGTCAACGG TTCGATTCTG
    TTATATGACC TTCGAAATAC AAGCTCTCAC GTCCAGGAAT TAGTACCTCA GAGAGCTAGA
    TGCCCGCTGG TATCCCTGTC GTATGTGCCC AGAGCTGCCT CTGCTGCGTT TCCATATGGT
    GGCGTGTTGG CTGGAGCGTT GGAGAATGCC TCTTTTTGGG AGCTGAAAAT GGGTTTTACT
    CATTGGCCTC ATGTGCTGCC CATGGAGCCT GGGGGCTGCG TAGACTTTCA GGTAGAGAGC
    AGCACCCGCC ACTGTCTCGT GACCTATAGG CCTGATAAAA ATCACAACAA CTTACGAAGT
    GTACTCATGG AGATGTCCTA TAAACTGGAT AATGCTGGAG AGCCCATCTG CTCCTGCCAT
    CCTGTACAAA CATTCTTTGG GGGACCCTCC TGCAAATTGC TAACCAAGAA TGCCATTTTC
    CAAAATCCAG AGAATAATGG CAGCATCCTG GTGTGTATTG GGGATGAAGC ATCAAAATCT
    GCCCTGCTGT GGGATGCTGG CAGTGGCTCA TTGCTGCAGG ATCTGCAGGC CGATCAGCCT
    GTCTTGGACA TCTGCCCATT TGACGTGAAT CATAACAGCT ACTTGGCTAC CTTAACAGAA
    AAGACCGTCC ACGTCTATAG GTGGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218437.1
    CDS140..2509
    Translation

    Target ORF information:

    RefSeq Version XM_005218380.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218380.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
    CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT
    CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG
    GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC
    AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA
    GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT
    AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC
    TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC
    CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA
    AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC
    TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG
    AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC
    ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG
    GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC
    CCCAAACAGT CTCCCCAGAA GTCTAACCCT TCTCTAACTT CTGCTTCTGT GGATGAGGAA
    GAAGGAGACA CTTGCACAAT ATGTTTGGAG CAGTGGACCA ATGCTGGGGA TCACCGGCTC
    TCTACATTGC GCTGTGGGCA CCTCTTTGGG TACAAATGCA TTTCGAAATG GCTCAGAGGA
    CAGACCCGAA AATGTCCCCA GTGTAACAAG AAAGCCAAGC AAAGTGATAT CGTTGTCCTT
    TATGCCCGGA CCTTGAGAGC TTTGGACACT AGTGAGCAGG AGCTCATGAA GAGTTCCTTA
    CTAAAGGAAC AGAAGCTAAG GAAGAAGGCT GAATTAGACT CGGCACAGTG TCGGCTGCAG
    CTTCAAGCCC TCACTGATAA ATGCTCTAGG CTTCAAAGTC GTGTTCAGGA CTTGCAGAAA
    CTTATCGGTC AGGATAAGAT CTCACAACAG CCTGGGGGTT CCCAACCACA TTTCCTAAGT
    GGCCTGCCCA CCAGCCAGAG TCAACAGAAG TACCATTTCC AGAAGACATT CACAGTGTCT
    CAAACAGGAA ACTGTCGAGT CATGACATAC TGTGATGCTT TGAGCTGCCT GGTGGTATCA
    CAACCTTCTC CTCAGTCCGC CTTCCTTCCA GGCTTTGGTG TTAAGATGTT GAGTACTGCC
    AACATGAAAA GCAGCCAGTA TATTCCTATG CATGGCAAAC AAATACGTGG ACTGGCTTTC
    AGCAGTCGAT CCAAAGGCTT ACTTCTCTCT GCTTCCCTGG ACAGCACAAT TAAACTGACC
    AGCCTGGAAA CAAATACCGT GGTCCAGACT TACAACGCTG GGCGGCCTGT CTGGAGCTGC
    TGCTGGTGTC TTGATGAAAA CAATTACATC TATGCTGGAC TGGTCAACGG TTCGATTCTG
    TTATATGACC TTCGAAATAC AAGCTCTCAC GTCCAGGAAT TAGTACCTCA GAGAGCTAGA
    TGCCCGCTGG TATCCCTGTC GTATGTGCCC AGAGCTGCCT CTGCTGCGTT TCCATATGGT
    GGCGTGTTGG CTGGAGCGTT GGAGAATGCC TCTTTTTGGG AGCTGAAAAT GGGTTTTACT
    CATTGGCCTC ATGTGCTGCC CATGGAGCCT GGGGGCTGCG TAGACTTTCA GGTAGAGAGC
    AGCACCCGCC ACTGTCTCGT GACCTATAGG CCTGATAAAA ATCACAACAA CTTACGAAGT
    GTACTCATGG AGATGTCCTA TAAACTGGAT AATGCTGGAG AGCCCATCTG CTCCTGCCAT
    CCTGTACAAA CATTCTTTGG GGGACCCTCC TGCAAATTGC TAACCAAGAA TGCCATTTTC
    CAAAATCCAG AGAATAATGG CAGCATCCTG GTGTGTATTG GGGATGAAGC ATCAAAATCT
    GCCCTGCTGT GGGATGCTGG CAGTGGCTCA TTGCTGCAGG ATCTGCAGGC CGATCAGCCT
    GTCTTGGACA TCTGCCCATT TGACGTGAAT CATAACAGCT ACTTGGCTAC CTTAACAGAA
    AAGACCGTCC ACGTCTATAG GTGGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09183
    Clone ID Related Accession (Same CDS sequence) XM_005195170.3 , XM_005218380.3 , XM_005218380.4
    Accession Version XM_005218380.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2370bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase RFWD3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218380.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
    CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT
    CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG
    GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC
    AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA
    GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT
    AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC
    TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC
    CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA
    AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC
    TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG
    AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC
    ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG
    GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC
    CCCAAACAGT CTCCCCAGAA GTCTAACCCT TCTCTAACTT CTGCTTCTGT GGATGAGGAA
    GAAGGAGACA CTTGCACAAT ATGTTTGGAG CAGTGGACCA ATGCTGGGGA TCACCGGCTC
    TCTACATTGC GCTGTGGGCA CCTCTTTGGG TACAAATGCA TTTCGAAATG GCTCAGAGGA
    CAGACCCGAA AATGTCCCCA GTGTAACAAG AAAGCCAAGC AAAGTGATAT CGTTGTCCTT
    TATGCCCGGA CCTTGAGAGC TTTGGACACT AGTGAGCAGG AGCTCATGAA GAGTTCCTTA
    CTAAAGGAAC AGAAGCTAAG GAAGAAGGCT GAATTAGACT CGGCACAGTG TCGGCTGCAG
    CTTCAAGCCC TCACTGATAA ATGCTCTAGG CTTCAAAGTC GTGTTCAGGA CTTGCAGAAA
    CTTATCGGTC AGGATAAGAT CTCACAACAG CCTGGGGGTT CCCAACCACA TTTCCTAAGT
    GGCCTGCCCA CCAGCCAGAG TCAACAGAAG TACCATTTCC AGAAGACATT CACAGTGTCT
    CAAACAGGAA ACTGTCGAGT CATGACATAC TGTGATGCTT TGAGCTGCCT GGTGGTATCA
    CAACCTTCTC CTCAGTCCGC CTTCCTTCCA GGCTTTGGTG TTAAGATGTT GAGTACTGCC
    AACATGAAAA GCAGCCAGTA TATTCCTATG CATGGCAAAC AAATACGTGG ACTGGCTTTC
    AGCAGTCGAT CCAAAGGCTT ACTTCTCTCT GCTTCCCTGG ACAGCACAAT TAAACTGACC
    AGCCTGGAAA CAAATACCGT GGTCCAGACT TACAACGCTG GGCGGCCTGT CTGGAGCTGC
    TGCTGGTGTC TTGATGAAAA CAATTACATC TATGCTGGAC TGGTCAACGG TTCGATTCTG
    TTATATGACC TTCGAAATAC AAGCTCTCAC GTCCAGGAAT TAGTACCTCA GAGAGCTAGA
    TGCCCGCTGG TATCCCTGTC GTATGTGCCC AGAGCTGCCT CTGCTGCGTT TCCATATGGT
    GGCGTGTTGG CTGGAGCGTT GGAGAATGCC TCTTTTTGGG AGCTGAAAAT GGGTTTTACT
    CATTGGCCTC ATGTGCTGCC CATGGAGCCT GGGGGCTGCG TAGACTTTCA GGTAGAGAGC
    AGCACCCGCC ACTGTCTCGT GACCTATAGG CCTGATAAAA ATCACAACAA CTTACGAAGT
    GTACTCATGG AGATGTCCTA TAAACTGGAT AATGCTGGAG AGCCCATCTG CTCCTGCCAT
    CCTGTACAAA CATTCTTTGG GGGACCCTCC TGCAAATTGC TAACCAAGAA TGCCATTTTC
    CAAAATCCAG AGAATAATGG CAGCATCCTG GTGTGTATTG GGGATGAAGC ATCAAAATCT
    GCCCTGCTGT GGGATGCTGG CAGTGGCTCA TTGCTGCAGG ATCTGCAGGC CGATCAGCCT
    GTCTTGGACA TCTGCCCATT TGACGTGAAT CATAACAGCT ACTTGGCTAC CTTAACAGAA
    AAGACCGTCC ACGTCTATAG GTGGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218437.1
    CDS157..2526
    Translation

    Target ORF information:

    RefSeq Version XM_005218380.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218380.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
    CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT
    CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG
    GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC
    AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA
    GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT
    AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC
    TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC
    CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA
    AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC
    TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG
    AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC
    ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG
    GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC
    CCCAAACAGT CTCCCCAGAA GTCTAACCCT TCTCTAACTT CTGCTTCTGT GGATGAGGAA
    GAAGGAGACA CTTGCACAAT ATGTTTGGAG CAGTGGACCA ATGCTGGGGA TCACCGGCTC
    TCTACATTGC GCTGTGGGCA CCTCTTTGGG TACAAATGCA TTTCGAAATG GCTCAGAGGA
    CAGACCCGAA AATGTCCCCA GTGTAACAAG AAAGCCAAGC AAAGTGATAT CGTTGTCCTT
    TATGCCCGGA CCTTGAGAGC TTTGGACACT AGTGAGCAGG AGCTCATGAA GAGTTCCTTA
    CTAAAGGAAC AGAAGCTAAG GAAGAAGGCT GAATTAGACT CGGCACAGTG TCGGCTGCAG
    CTTCAAGCCC TCACTGATAA ATGCTCTAGG CTTCAAAGTC GTGTTCAGGA CTTGCAGAAA
    CTTATCGGTC AGGATAAGAT CTCACAACAG CCTGGGGGTT CCCAACCACA TTTCCTAAGT
    GGCCTGCCCA CCAGCCAGAG TCAACAGAAG TACCATTTCC AGAAGACATT CACAGTGTCT
    CAAACAGGAA ACTGTCGAGT CATGACATAC TGTGATGCTT TGAGCTGCCT GGTGGTATCA
    CAACCTTCTC CTCAGTCCGC CTTCCTTCCA GGCTTTGGTG TTAAGATGTT GAGTACTGCC
    AACATGAAAA GCAGCCAGTA TATTCCTATG CATGGCAAAC AAATACGTGG ACTGGCTTTC
    AGCAGTCGAT CCAAAGGCTT ACTTCTCTCT GCTTCCCTGG ACAGCACAAT TAAACTGACC
    AGCCTGGAAA CAAATACCGT GGTCCAGACT TACAACGCTG GGCGGCCTGT CTGGAGCTGC
    TGCTGGTGTC TTGATGAAAA CAATTACATC TATGCTGGAC TGGTCAACGG TTCGATTCTG
    TTATATGACC TTCGAAATAC AAGCTCTCAC GTCCAGGAAT TAGTACCTCA GAGAGCTAGA
    TGCCCGCTGG TATCCCTGTC GTATGTGCCC AGAGCTGCCT CTGCTGCGTT TCCATATGGT
    GGCGTGTTGG CTGGAGCGTT GGAGAATGCC TCTTTTTGGG AGCTGAAAAT GGGTTTTACT
    CATTGGCCTC ATGTGCTGCC CATGGAGCCT GGGGGCTGCG TAGACTTTCA GGTAGAGAGC
    AGCACCCGCC ACTGTCTCGT GACCTATAGG CCTGATAAAA ATCACAACAA CTTACGAAGT
    GTACTCATGG AGATGTCCTA TAAACTGGAT AATGCTGGAG AGCCCATCTG CTCCTGCCAT
    CCTGTACAAA CATTCTTTGG GGGACCCTCC TGCAAATTGC TAACCAAGAA TGCCATTTTC
    CAAAATCCAG AGAATAATGG CAGCATCCTG GTGTGTATTG GGGATGAAGC ATCAAAATCT
    GCCCTGCTGT GGGATGCTGG CAGTGGCTCA TTGCTGCAGG ATCTGCAGGC CGATCAGCCT
    GTCTTGGACA TCTGCCCATT TGACGTGAAT CATAACAGCT ACTTGGCTAC CTTAACAGAA
    AAGACCGTCC ACGTCTATAG GTGGGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.