RFWD3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RFWD3
Entrez Gene ID	540072
Full Name	ring finger and WD repeat domain 3
General protein information	Preferred Names ring finger and WD repeat domain 3 Names E3 ubiquitin-protein ligase RFWD3
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005195170.3	XP_005195227.1	E3 ubiquitin-protein ligase RFWD3 isoform X2
XM_002694723.6	XP_002694769.1	E3 ubiquitin-protein ligase RFWD3 isoform X1
XM_002694723.5	XP_002694769.1	E3 ubiquitin-protein ligase RFWD3 isoform X1
XM_005218380.3	XP_005218437.1	E3 ubiquitin-protein ligase RFWD3 isoform X2
XM_591339.8	XP_591339.3	E3 ubiquitin-protein ligase RFWD3 isoform X1
XM_005218380.4	XP_005218437.1	E3 ubiquitin-protein ligase RFWD3 isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rfwd3	XP_006255701.1
	RFWD3	XP_002802615.1
Danio rerio (zebrafish)	LOC100329327	XP_002667008.1
Drosophila melanogaster (fruit fly)	CG13025	NP_001261971.1
Homo sapiens (human)	RFWD3	NP_060594.3
Mus musculus (house mouse)	Rfwd3	NP_666330.2
Pan troglodytes (chimpanzee)	RFWD3	XP_001137409.1
Canis lupus familiaris (dog)	RFWD3	XP_853024.2
Bos taurus (cattle)	RFWD3	XP_005218437.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005643	XP_315661.2
Xenopus tropicalis (tropical clawed frog)	rfwd3	XP_002931710.1
Gallus gallus (chicken)	RFWD3	XP_414051.3

Gene Ontology

The following RFWD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFWD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa09182	XM_002694723.6 Latest version!	Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa09182	XM_591339.8 Latest version!	Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa09183	XM_005195170.3 Latest version!	Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa09183	XM_005218380.4 Latest version!	Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OBa09182	XM_002694723.5	Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa09183	XM_005218380.3	Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RFWD3 ( XM_002694723.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002694769.1 Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa09182
Clone ID Related Accession (Same CDS sequence)	XM_002694723.6 , XM_002694723.5 , XM_591339.8
Accession Version	XM_002694723.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002694723.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC 
TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC 
CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA 
AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC 
TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG 
AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC 
ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG 
GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC 
CCCAAACAGC AGTCTCCCCA GAAGTCTAAC CCTTCTCTAA CTTCTGCTTC TGTGGATGAG 
GAAGAAGGAG ACACTTGCAC AATATGTTTG GAGCAGTGGA CCAATGCTGG GGATCACCGG 
CTCTCTACAT TGCGCTGTGG GCACCTCTTT GGGTACAAAT GCATTTCGAA ATGGCTCAGA 
GGACAGACCC GAAAATGTCC CCAGTGTAAC AAGAAAGCCA AGCAAAGTGA TATCGTTGTC 
CTTTATGCCC GGACCTTGAG AGCTTTGGAC ACTAGTGAGC AGGAGCTCAT GAAGAGTTCC 
TTACTAAAGG AACAGAAGCT AAGGAAGAAG GCTGAATTAG ACTCGGCACA GTGTCGGCTG 
CAGCTTCAAG CCCTCACTGA TAAATGCTCT AGGCTTCAAA GTCGTGTTCA GGACTTGCAG 
AAACTTATCG GTCAGGATAA GATCTCACAA CAGCCTGGGG GTTCCCAACC ACATTTCCTA 
AGTGGCCTGC CCACCAGCCA GAGTCAACAG AAGTACCATT TCCAGAAGAC ATTCACAGTG 
TCTCAAACAG GAAACTGTCG AGTCATGACA TACTGTGATG CTTTGAGCTG CCTGGTGGTA 
TCACAACCTT CTCCTCAGTC CGCCTTCCTT CCAGGCTTTG GTGTTAAGAT GTTGAGTACT 
GCCAACATGA AAAGCAGCCA GTATATTCCT ATGCATGGCA AACAAATACG TGGACTGGCT 
TTCAGCAGTC GATCCAAAGG CTTACTTCTC TCTGCTTCCC TGGACAGCAC AATTAAACTG 
ACCAGCCTGG AAACAAATAC CGTGGTCCAG ACTTACAACG CTGGGCGGCC TGTCTGGAGC 
TGCTGCTGGT GTCTTGATGA AAACAATTAC ATCTATGCTG GACTGGTCAA CGGTTCGATT 
CTGTTATATG ACCTTCGAAA TACAAGCTCT CACGTCCAGG AATTAGTACC TCAGAGAGCT 
AGATGCCCGC TGGTATCCCT GTCGTATGTG CCCAGAGCTG CCTCTGCTGC GTTTCCATAT 
GGTGGCGTGT TGGCTGGAGC GTTGGAGAAT GCCTCTTTTT GGGAGCTGAA AATGGGTTTT 
ACTCATTGGC CTCATGTGCT GCCCATGGAG CCTGGGGGCT GCGTAGACTT TCAGGTAGAG 
AGCAGCACCC GCCACTGTCT CGTGACCTAT AGGCCTGATA AAAATCACAA CAACTTACGA 
AGTGTACTCA TGGAGATGTC CTATAAACTG GATAATGCTG GAGAGCCCAT CTGCTCCTGC 
CATCCTGTAC AAACATTCTT TGGGGGACCC TCCTGCAAAT TGCTAACCAA GAATGCCATT 
TTCCAAAATC CAGAGAATAA TGGCAGCATC CTGGTGTGTA TTGGGGATGA AGCATCAAAA 
TCTGCCCTGC TGTGGGATGC TGGCAGTGGC TCATTGCTGC AGGATCTGCA GGCCGATCAG 
CCTGTCTTGG ACATCTGCCC ATTTGACGTG AATCATAACA GCTACTTGGC TACCTTAACA 
GAAAAGACCG TCCACGTCTA TAGGTGGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002694769.1
CDS	157..2529
Translation	MAHEAMEYDLQVQLDHGAAEQPAPAEVVSSQGGPPLLQPPHTEEVSSQEGPPMLHPVLAEVVSIEGGPPLLQPIPEELINSPERVPLLPPIPQIPIDLEEVELSETETVGNLNPGVLEEFRPRSGANHTPQVTSLDSMNSFISGLQRLHGMLEFLRPTSDRRVGPVRARRSRRSSASRRARAGGSQRTNSARLRAPLDAFFQASRTQPHSSTTSYDSETRNLVSDDLQVSGSSDSDSDSSTEFEEGVAQAEEPEAVVLEEELEDTSTDQEVICAGGEDTVPKQQSPQKSNPSLTSASVDEEEGDTCTICLEQWTNAGDHRLSTLRCGHLFGYKCISKWLRGQTRKCPQCNKKAKQSDIVVLYARTLRALDTSEQELMKSSLLKEQKLRKKAELDSAQCRLQLQALTDKCSRLQSRVQDLQKLIGQDKISQQPGGSQPHFLSGLPTSQSQQKYHFQKTFTVSQTGNCRVMTYCDALSCLVVSQPSPQSAFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSRSKGLLLSASLDSTIKLTSLETNTVVQTYNAGRPVWSCCWCLDENNYIYAGLVNGSILLYDLRNTSSHVQELVPQRARCPLVSLSYVPRAASAAFPYGGVLAGALENASFWELKMGFTHWPHVLPMEPGGCVDFQVESSTRHCLVTYRPDKNHNNLRSVLMEMSYKLDNAGEPICSCHPVQTFFGGPSCKLLTKNAIFQNPENNGSILVCIGDEASKSALLWDAGSGSLLQDLQADQPVLDICPFDVNHNSYLATLTEKTVHVYRWE

Target ORF information:

RefSeq Version	XM_002694723.6
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002694723.6

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC 
TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC 
CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA 
AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC 
TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG 
AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC 
ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG 
GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC 
CCCAAACAGC AGTCTCCCCA GAAGTCTAAC CCTTCTCTAA CTTCTGCTTC TGTGGATGAG 
GAAGAAGGAG ACACTTGCAC AATATGTTTG GAGCAGTGGA CCAATGCTGG GGATCACCGG 
CTCTCTACAT TGCGCTGTGG GCACCTCTTT GGGTACAAAT GCATTTCGAA ATGGCTCAGA 
GGACAGACCC GAAAATGTCC CCAGTGTAAC AAGAAAGCCA AGCAAAGTGA TATCGTTGTC 
CTTTATGCCC GGACCTTGAG AGCTTTGGAC ACTAGTGAGC AGGAGCTCAT GAAGAGTTCC 
TTACTAAAGG AACAGAAGCT AAGGAAGAAG GCTGAATTAG ACTCGGCACA GTGTCGGCTG 
CAGCTTCAAG CCCTCACTGA TAAATGCTCT AGGCTTCAAA GTCGTGTTCA GGACTTGCAG 
AAACTTATCG GTCAGGATAA GATCTCACAA CAGCCTGGGG GTTCCCAACC ACATTTCCTA 
AGTGGCCTGC CCACCAGCCA GAGTCAACAG AAGTACCATT TCCAGAAGAC ATTCACAGTG 
TCTCAAACAG GAAACTGTCG AGTCATGACA TACTGTGATG CTTTGAGCTG CCTGGTGGTA 
TCACAACCTT CTCCTCAGTC CGCCTTCCTT CCAGGCTTTG GTGTTAAGAT GTTGAGTACT 
GCCAACATGA AAAGCAGCCA GTATATTCCT ATGCATGGCA AACAAATACG TGGACTGGCT 
TTCAGCAGTC GATCCAAAGG CTTACTTCTC TCTGCTTCCC TGGACAGCAC AATTAAACTG 
ACCAGCCTGG AAACAAATAC CGTGGTCCAG ACTTACAACG CTGGGCGGCC TGTCTGGAGC 
TGCTGCTGGT GTCTTGATGA AAACAATTAC ATCTATGCTG GACTGGTCAA CGGTTCGATT 
CTGTTATATG ACCTTCGAAA TACAAGCTCT CACGTCCAGG AATTAGTACC TCAGAGAGCT 
AGATGCCCGC TGGTATCCCT GTCGTATGTG CCCAGAGCTG CCTCTGCTGC GTTTCCATAT 
GGTGGCGTGT TGGCTGGAGC GTTGGAGAAT GCCTCTTTTT GGGAGCTGAA AATGGGTTTT 
ACTCATTGGC CTCATGTGCT GCCCATGGAG CCTGGGGGCT GCGTAGACTT TCAGGTAGAG 
AGCAGCACCC GCCACTGTCT CGTGACCTAT AGGCCTGATA AAAATCACAA CAACTTACGA 
AGTGTACTCA TGGAGATGTC CTATAAACTG GATAATGCTG GAGAGCCCAT CTGCTCCTGC 
CATCCTGTAC AAACATTCTT TGGGGGACCC TCCTGCAAAT TGCTAACCAA GAATGCCATT 
TTCCAAAATC CAGAGAATAA TGGCAGCATC CTGGTGTGTA TTGGGGATGA AGCATCAAAA 
TCTGCCCTGC TGTGGGATGC TGGCAGTGGC TCATTGCTGC AGGATCTGCA GGCCGATCAG 
CCTGTCTTGG ACATCTGCCC ATTTGACGTG AATCATAACA GCTACTTGGC TACCTTAACA 
GAAAAGACCG TCCACGTCTA TAGGTGGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_002694723.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002694769.1 Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa09182
Clone ID Related Accession (Same CDS sequence)	XM_002694723.6 , XM_002694723.5 , XM_591339.8
Accession Version	XM_002694723.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104462.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002694723.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC 
TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC 
CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA 
AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC 
TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG 
AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC 
ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG 
GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC 
CCCAAACAGC AGTCTCCCCA GAAGTCTAAC CCTTCTCTAA CTTCTGCTTC TGTGGATGAG 
GAAGAAGGAG ACACTTGCAC AATATGTTTG GAGCAGTGGA CCAATGCTGG GGATCACCGG 
CTCTCTACAT TGCGCTGTGG GCACCTCTTT GGGTACAAAT GCATTTCGAA ATGGCTCAGA 
GGACAGACCC GAAAATGTCC CCAGTGTAAC AAGAAAGCCA AGCAAAGTGA TATCGTTGTC 
CTTTATGCCC GGACCTTGAG AGCTTTGGAC ACTAGTGAGC AGGAGCTCAT GAAGAGTTCC 
TTACTAAAGG AACAGAAGCT AAGGAAGAAG GCTGAATTAG ACTCGGCACA GTGTCGGCTG 
CAGCTTCAAG CCCTCACTGA TAAATGCTCT AGGCTTCAAA GTCGTGTTCA GGACTTGCAG 
AAACTTATCG GTCAGGATAA GATCTCACAA CAGCCTGGGG GTTCCCAACC ACATTTCCTA 
AGTGGCCTGC CCACCAGCCA GAGTCAACAG AAGTACCATT TCCAGAAGAC ATTCACAGTG 
TCTCAAACAG GAAACTGTCG AGTCATGACA TACTGTGATG CTTTGAGCTG CCTGGTGGTA 
TCACAACCTT CTCCTCAGTC CGCCTTCCTT CCAGGCTTTG GTGTTAAGAT GTTGAGTACT 
GCCAACATGA AAAGCAGCCA GTATATTCCT ATGCATGGCA AACAAATACG TGGACTGGCT 
TTCAGCAGTC GATCCAAAGG CTTACTTCTC TCTGCTTCCC TGGACAGCAC AATTAAACTG 
ACCAGCCTGG AAACAAATAC CGTGGTCCAG ACTTACAACG CTGGGCGGCC TGTCTGGAGC 
TGCTGCTGGT GTCTTGATGA AAACAATTAC ATCTATGCTG GACTGGTCAA CGGTTCGATT 
CTGTTATATG ACCTTCGAAA TACAAGCTCT CACGTCCAGG AATTAGTACC TCAGAGAGCT 
AGATGCCCGC TGGTATCCCT GTCGTATGTG CCCAGAGCTG CCTCTGCTGC GTTTCCATAT 
GGTGGCGTGT TGGCTGGAGC GTTGGAGAAT GCCTCTTTTT GGGAGCTGAA AATGGGTTTT 
ACTCATTGGC CTCATGTGCT GCCCATGGAG CCTGGGGGCT GCGTAGACTT TCAGGTAGAG 
AGCAGCACCC GCCACTGTCT CGTGACCTAT AGGCCTGATA AAAATCACAA CAACTTACGA 
AGTGTACTCA TGGAGATGTC CTATAAACTG GATAATGCTG GAGAGCCCAT CTGCTCCTGC 
CATCCTGTAC AAACATTCTT TGGGGGACCC TCCTGCAAAT TGCTAACCAA GAATGCCATT 
TTCCAAAATC CAGAGAATAA TGGCAGCATC CTGGTGTGTA TTGGGGATGA AGCATCAAAA 
TCTGCCCTGC TGTGGGATGC TGGCAGTGGC TCATTGCTGC AGGATCTGCA GGCCGATCAG 
CCTGTCTTGG ACATCTGCCC ATTTGACGTG AATCATAACA GCTACTTGGC TACCTTAACA 
GAAAAGACCG TCCACGTCTA TAGGTGGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002694769.1
CDS	140..2512
Translation	MAHEAMEYDLQVQLDHGAAEQPAPAEVVSSQGGPPLLQPPHTEEVSSQEGPPMLHPVLAEVVSIEGGPPLLQPIPEELINSPERVPLLPPIPQIPIDLEEVELSETETVGNLNPGVLEEFRPRSGANHTPQVTSLDSMNSFISGLQRLHGMLEFLRPTSDRRVGPVRARRSRRSSASRRARAGGSQRTNSARLRAPLDAFFQASRTQPHSSTTSYDSETRNLVSDDLQVSGSSDSDSDSSTEFEEGVAQAEEPEAVVLEEELEDTSTDQEVICAGGEDTVPKQQSPQKSNPSLTSASVDEEEGDTCTICLEQWTNAGDHRLSTLRCGHLFGYKCISKWLRGQTRKCPQCNKKAKQSDIVVLYARTLRALDTSEQELMKSSLLKEQKLRKKAELDSAQCRLQLQALTDKCSRLQSRVQDLQKLIGQDKISQQPGGSQPHFLSGLPTSQSQQKYHFQKTFTVSQTGNCRVMTYCDALSCLVVSQPSPQSAFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSRSKGLLLSASLDSTIKLTSLETNTVVQTYNAGRPVWSCCWCLDENNYIYAGLVNGSILLYDLRNTSSHVQELVPQRARCPLVSLSYVPRAASAAFPYGGVLAGALENASFWELKMGFTHWPHVLPMEPGGCVDFQVESSTRHCLVTYRPDKNHNNLRSVLMEMSYKLDNAGEPICSCHPVQTFFGGPSCKLLTKNAIFQNPENNGSILVCIGDEASKSALLWDAGSGSLLQDLQADQPVLDICPFDVNHNSYLATLTEKTVHVYRWE

Target ORF information:

RefSeq Version	XM_002694723.5
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002694723.5

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC 
TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC 
CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA 
AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC 
TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG 
AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC 
ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG 
GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC 
CCCAAACAGC AGTCTCCCCA GAAGTCTAAC CCTTCTCTAA CTTCTGCTTC TGTGGATGAG 
GAAGAAGGAG ACACTTGCAC AATATGTTTG GAGCAGTGGA CCAATGCTGG GGATCACCGG 
CTCTCTACAT TGCGCTGTGG GCACCTCTTT GGGTACAAAT GCATTTCGAA ATGGCTCAGA 
GGACAGACCC GAAAATGTCC CCAGTGTAAC AAGAAAGCCA AGCAAAGTGA TATCGTTGTC 
CTTTATGCCC GGACCTTGAG AGCTTTGGAC ACTAGTGAGC AGGAGCTCAT GAAGAGTTCC 
TTACTAAAGG AACAGAAGCT AAGGAAGAAG GCTGAATTAG ACTCGGCACA GTGTCGGCTG 
CAGCTTCAAG CCCTCACTGA TAAATGCTCT AGGCTTCAAA GTCGTGTTCA GGACTTGCAG 
AAACTTATCG GTCAGGATAA GATCTCACAA CAGCCTGGGG GTTCCCAACC ACATTTCCTA 
AGTGGCCTGC CCACCAGCCA GAGTCAACAG AAGTACCATT TCCAGAAGAC ATTCACAGTG 
TCTCAAACAG GAAACTGTCG AGTCATGACA TACTGTGATG CTTTGAGCTG CCTGGTGGTA 
TCACAACCTT CTCCTCAGTC CGCCTTCCTT CCAGGCTTTG GTGTTAAGAT GTTGAGTACT 
GCCAACATGA AAAGCAGCCA GTATATTCCT ATGCATGGCA AACAAATACG TGGACTGGCT 
TTCAGCAGTC GATCCAAAGG CTTACTTCTC TCTGCTTCCC TGGACAGCAC AATTAAACTG 
ACCAGCCTGG AAACAAATAC CGTGGTCCAG ACTTACAACG CTGGGCGGCC TGTCTGGAGC 
TGCTGCTGGT GTCTTGATGA AAACAATTAC ATCTATGCTG GACTGGTCAA CGGTTCGATT 
CTGTTATATG ACCTTCGAAA TACAAGCTCT CACGTCCAGG AATTAGTACC TCAGAGAGCT 
AGATGCCCGC TGGTATCCCT GTCGTATGTG CCCAGAGCTG CCTCTGCTGC GTTTCCATAT 
GGTGGCGTGT TGGCTGGAGC GTTGGAGAAT GCCTCTTTTT GGGAGCTGAA AATGGGTTTT 
ACTCATTGGC CTCATGTGCT GCCCATGGAG CCTGGGGGCT GCGTAGACTT TCAGGTAGAG 
AGCAGCACCC GCCACTGTCT CGTGACCTAT AGGCCTGATA AAAATCACAA CAACTTACGA 
AGTGTACTCA TGGAGATGTC CTATAAACTG GATAATGCTG GAGAGCCCAT CTGCTCCTGC 
CATCCTGTAC AAACATTCTT TGGGGGACCC TCCTGCAAAT TGCTAACCAA GAATGCCATT 
TTCCAAAATC CAGAGAATAA TGGCAGCATC CTGGTGTGTA TTGGGGATGA AGCATCAAAA 
TCTGCCCTGC TGTGGGATGC TGGCAGTGGC TCATTGCTGC AGGATCTGCA GGCCGATCAG 
CCTGTCTTGG ACATCTGCCC ATTTGACGTG AATCATAACA GCTACTTGGC TACCTTAACA 
GAAAAGACCG TCCACGTCTA TAGGTGGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_591339.8 ) cDNA ORF clone, Bos taurus(cattle) -> XP_591339.3 Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa09182
Clone ID Related Accession (Same CDS sequence)	XM_002694723.6 , XM_002694723.5 , XM_591339.8
Accession Version	XM_591339.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646378.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_591339.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC 
TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC 
CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA 
AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC 
TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG 
AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC 
ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG 
GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC 
CCCAAACAGC AGTCTCCCCA GAAGTCTAAC CCTTCTCTAA CTTCTGCTTC TGTGGATGAG 
GAAGAAGGAG ACACTTGCAC AATATGTTTG GAGCAGTGGA CCAATGCTGG GGATCACCGG 
CTCTCTACAT TGCGCTGTGG GCACCTCTTT GGGTACAAAT GCATTTCGAA ATGGCTCAGA 
GGACAGACCC GAAAATGTCC CCAGTGTAAC AAGAAAGCCA AGCAAAGTGA TATCGTTGTC 
CTTTATGCCC GGACCTTGAG AGCTTTGGAC ACTAGTGAGC AGGAGCTCAT GAAGAGTTCC 
TTACTAAAGG AACAGAAGCT AAGGAAGAAG GCTGAATTAG ACTCGGCACA GTGTCGGCTG 
CAGCTTCAAG CCCTCACTGA TAAATGCTCT AGGCTTCAAA GTCGTGTTCA GGACTTGCAG 
AAACTTATCG GTCAGGATAA GATCTCACAA CAGCCTGGGG GTTCCCAACC ACATTTCCTA 
AGTGGCCTGC CCACCAGCCA GAGTCAACAG AAGTACCATT TCCAGAAGAC ATTCACAGTG 
TCTCAAACAG GAAACTGTCG AGTCATGACA TACTGTGATG CTTTGAGCTG CCTGGTGGTA 
TCACAACCTT CTCCTCAGTC CGCCTTCCTT CCAGGCTTTG GTGTTAAGAT GTTGAGTACT 
GCCAACATGA AAAGCAGCCA GTATATTCCT ATGCATGGCA AACAAATACG TGGACTGGCT 
TTCAGCAGTC GATCCAAAGG CTTACTTCTC TCTGCTTCCC TGGACAGCAC AATTAAACTG 
ACCAGCCTGG AAACAAATAC CGTGGTCCAG ACTTACAACG CTGGGCGGCC TGTCTGGAGC 
TGCTGCTGGT GTCTTGATGA AAACAATTAC ATCTATGCTG GACTGGTCAA CGGTTCGATT 
CTGTTATATG ACCTTCGAAA TACAAGCTCT CACGTCCAGG AATTAGTACC TCAGAGAGCT 
AGATGCCCGC TGGTATCCCT GTCGTATGTG CCCAGAGCTG CCTCTGCTGC GTTTCCATAT 
GGTGGCGTGT TGGCTGGAGC GTTGGAGAAT GCCTCTTTTT GGGAGCTGAA AATGGGTTTT 
ACTCATTGGC CTCATGTGCT GCCCATGGAG CCTGGGGGCT GCGTAGACTT TCAGGTAGAG 
AGCAGCACCC GCCACTGTCT CGTGACCTAT AGGCCTGATA AAAATCACAA CAACTTACGA 
AGTGTACTCA TGGAGATGTC CTATAAACTG GATAATGCTG GAGAGCCCAT CTGCTCCTGC 
CATCCTGTAC AAACATTCTT TGGGGGACCC TCCTGCAAAT TGCTAACCAA GAATGCCATT 
TTCCAAAATC CAGAGAATAA TGGCAGCATC CTGGTGTGTA TTGGGGATGA AGCATCAAAA 
TCTGCCCTGC TGTGGGATGC TGGCAGTGGC TCATTGCTGC AGGATCTGCA GGCCGATCAG 
CCTGTCTTGG ACATCTGCCC ATTTGACGTG AATCATAACA GCTACTTGGC TACCTTAACA 
GAAAAGACCG TCCACGTCTA TAGGTGGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_591339.3
CDS	140..2512
Translation	MAHEAMEYDLQVQLDHGAAEQPAPAEVVSSQGGPPLLQPPHTEEVSSQEGPPMLHPVLAEVVSIEGGPPLLQPIPEELINSPERVPLLPPIPQIPIDLEEVELSETETVGNLNPGVLEEFRPRSGANHTPQVTSLDSMNSFISGLQRLHGMLEFLRPTSDRRVGPVRARRSRRSSASRRARAGGSQRTNSARLRAPLDAFFQASRTQPHSSTTSYDSETRNLVSDDLQVSGSSDSDSDSSTEFEEGVAQAEEPEAVVLEEELEDTSTDQEVICAGGEDTVPKQQSPQKSNPSLTSASVDEEEGDTCTICLEQWTNAGDHRLSTLRCGHLFGYKCISKWLRGQTRKCPQCNKKAKQSDIVVLYARTLRALDTSEQELMKSSLLKEQKLRKKAELDSAQCRLQLQALTDKCSRLQSRVQDLQKLIGQDKISQQPGGSQPHFLSGLPTSQSQQKYHFQKTFTVSQTGNCRVMTYCDALSCLVVSQPSPQSAFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSRSKGLLLSASLDSTIKLTSLETNTVVQTYNAGRPVWSCCWCLDENNYIYAGLVNGSILLYDLRNTSSHVQELVPQRARCPLVSLSYVPRAASAAFPYGGVLAGALENASFWELKMGFTHWPHVLPMEPGGCVDFQVESSTRHCLVTYRPDKNHNNLRSVLMEMSYKLDNAGEPICSCHPVQTFFGGPSCKLLTKNAIFQNPENNGSILVCIGDEASKSALLWDAGSGSLLQDLQADQPVLDICPFDVNHNSYLATLTEKTVHVYRWE

Target ORF information:

RefSeq Version	XM_591339.8
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_591339.8

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC 
TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC 
CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA 
AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC 
TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG 
AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC 
ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG 
GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC 
CCCAAACAGC AGTCTCCCCA GAAGTCTAAC CCTTCTCTAA CTTCTGCTTC TGTGGATGAG 
GAAGAAGGAG ACACTTGCAC AATATGTTTG GAGCAGTGGA CCAATGCTGG GGATCACCGG 
CTCTCTACAT TGCGCTGTGG GCACCTCTTT GGGTACAAAT GCATTTCGAA ATGGCTCAGA 
GGACAGACCC GAAAATGTCC CCAGTGTAAC AAGAAAGCCA AGCAAAGTGA TATCGTTGTC 
CTTTATGCCC GGACCTTGAG AGCTTTGGAC ACTAGTGAGC AGGAGCTCAT GAAGAGTTCC 
TTACTAAAGG AACAGAAGCT AAGGAAGAAG GCTGAATTAG ACTCGGCACA GTGTCGGCTG 
CAGCTTCAAG CCCTCACTGA TAAATGCTCT AGGCTTCAAA GTCGTGTTCA GGACTTGCAG 
AAACTTATCG GTCAGGATAA GATCTCACAA CAGCCTGGGG GTTCCCAACC ACATTTCCTA 
AGTGGCCTGC CCACCAGCCA GAGTCAACAG AAGTACCATT TCCAGAAGAC ATTCACAGTG 
TCTCAAACAG GAAACTGTCG AGTCATGACA TACTGTGATG CTTTGAGCTG CCTGGTGGTA 
TCACAACCTT CTCCTCAGTC CGCCTTCCTT CCAGGCTTTG GTGTTAAGAT GTTGAGTACT 
GCCAACATGA AAAGCAGCCA GTATATTCCT ATGCATGGCA AACAAATACG TGGACTGGCT 
TTCAGCAGTC GATCCAAAGG CTTACTTCTC TCTGCTTCCC TGGACAGCAC AATTAAACTG 
ACCAGCCTGG AAACAAATAC CGTGGTCCAG ACTTACAACG CTGGGCGGCC TGTCTGGAGC 
TGCTGCTGGT GTCTTGATGA AAACAATTAC ATCTATGCTG GACTGGTCAA CGGTTCGATT 
CTGTTATATG ACCTTCGAAA TACAAGCTCT CACGTCCAGG AATTAGTACC TCAGAGAGCT 
AGATGCCCGC TGGTATCCCT GTCGTATGTG CCCAGAGCTG CCTCTGCTGC GTTTCCATAT 
GGTGGCGTGT TGGCTGGAGC GTTGGAGAAT GCCTCTTTTT GGGAGCTGAA AATGGGTTTT 
ACTCATTGGC CTCATGTGCT GCCCATGGAG CCTGGGGGCT GCGTAGACTT TCAGGTAGAG 
AGCAGCACCC GCCACTGTCT CGTGACCTAT AGGCCTGATA AAAATCACAA CAACTTACGA 
AGTGTACTCA TGGAGATGTC CTATAAACTG GATAATGCTG GAGAGCCCAT CTGCTCCTGC 
CATCCTGTAC AAACATTCTT TGGGGGACCC TCCTGCAAAT TGCTAACCAA GAATGCCATT 
TTCCAAAATC CAGAGAATAA TGGCAGCATC CTGGTGTGTA TTGGGGATGA AGCATCAAAA 
TCTGCCCTGC TGTGGGATGC TGGCAGTGGC TCATTGCTGC AGGATCTGCA GGCCGATCAG 
CCTGTCTTGG ACATCTGCCC ATTTGACGTG AATCATAACA GCTACTTGGC TACCTTAACA 
GAAAAGACCG TCCACGTCTA TAGGTGGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_005195170.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005195227.1 Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa09183
Clone ID Related Accession (Same CDS sequence)	XM_005195170.3 , XM_005218380.3 , XM_005218380.4
Accession Version	XM_005195170.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646378.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195170.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC 
TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC 
CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA 
AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC 
TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG 
AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC 
ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG 
GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC 
CCCAAACAGT CTCCCCAGAA GTCTAACCCT TCTCTAACTT CTGCTTCTGT GGATGAGGAA 
GAAGGAGACA CTTGCACAAT ATGTTTGGAG CAGTGGACCA ATGCTGGGGA TCACCGGCTC 
TCTACATTGC GCTGTGGGCA CCTCTTTGGG TACAAATGCA TTTCGAAATG GCTCAGAGGA 
CAGACCCGAA AATGTCCCCA GTGTAACAAG AAAGCCAAGC AAAGTGATAT CGTTGTCCTT 
TATGCCCGGA CCTTGAGAGC TTTGGACACT AGTGAGCAGG AGCTCATGAA GAGTTCCTTA 
CTAAAGGAAC AGAAGCTAAG GAAGAAGGCT GAATTAGACT CGGCACAGTG TCGGCTGCAG 
CTTCAAGCCC TCACTGATAA ATGCTCTAGG CTTCAAAGTC GTGTTCAGGA CTTGCAGAAA 
CTTATCGGTC AGGATAAGAT CTCACAACAG CCTGGGGGTT CCCAACCACA TTTCCTAAGT 
GGCCTGCCCA CCAGCCAGAG TCAACAGAAG TACCATTTCC AGAAGACATT CACAGTGTCT 
CAAACAGGAA ACTGTCGAGT CATGACATAC TGTGATGCTT TGAGCTGCCT GGTGGTATCA 
CAACCTTCTC CTCAGTCCGC CTTCCTTCCA GGCTTTGGTG TTAAGATGTT GAGTACTGCC 
AACATGAAAA GCAGCCAGTA TATTCCTATG CATGGCAAAC AAATACGTGG ACTGGCTTTC 
AGCAGTCGAT CCAAAGGCTT ACTTCTCTCT GCTTCCCTGG ACAGCACAAT TAAACTGACC 
AGCCTGGAAA CAAATACCGT GGTCCAGACT TACAACGCTG GGCGGCCTGT CTGGAGCTGC 
TGCTGGTGTC TTGATGAAAA CAATTACATC TATGCTGGAC TGGTCAACGG TTCGATTCTG 
TTATATGACC TTCGAAATAC AAGCTCTCAC GTCCAGGAAT TAGTACCTCA GAGAGCTAGA 
TGCCCGCTGG TATCCCTGTC GTATGTGCCC AGAGCTGCCT CTGCTGCGTT TCCATATGGT 
GGCGTGTTGG CTGGAGCGTT GGAGAATGCC TCTTTTTGGG AGCTGAAAAT GGGTTTTACT 
CATTGGCCTC ATGTGCTGCC CATGGAGCCT GGGGGCTGCG TAGACTTTCA GGTAGAGAGC 
AGCACCCGCC ACTGTCTCGT GACCTATAGG CCTGATAAAA ATCACAACAA CTTACGAAGT 
GTACTCATGG AGATGTCCTA TAAACTGGAT AATGCTGGAG AGCCCATCTG CTCCTGCCAT 
CCTGTACAAA CATTCTTTGG GGGACCCTCC TGCAAATTGC TAACCAAGAA TGCCATTTTC 
CAAAATCCAG AGAATAATGG CAGCATCCTG GTGTGTATTG GGGATGAAGC ATCAAAATCT 
GCCCTGCTGT GGGATGCTGG CAGTGGCTCA TTGCTGCAGG ATCTGCAGGC CGATCAGCCT 
GTCTTGGACA TCTGCCCATT TGACGTGAAT CATAACAGCT ACTTGGCTAC CTTAACAGAA 
AAGACCGTCC ACGTCTATAG GTGGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005195227.1
CDS	140..2509
Translation	MAHEAMEYDLQVQLDHGAAEQPAPAEVVSSQGGPPLLQPPHTEEVSSQEGPPMLHPVLAEVVSIEGGPPLLQPIPEELINSPERVPLLPPIPQIPIDLEEVELSETETVGNLNPGVLEEFRPRSGANHTPQVTSLDSMNSFISGLQRLHGMLEFLRPTSDRRVGPVRARRSRRSSASRRARAGGSQRTNSARLRAPLDAFFQASRTQPHSSTTSYDSETRNLVSDDLQVSGSSDSDSDSSTEFEEGVAQAEEPEAVVLEEELEDTSTDQEVICAGGEDTVPKQSPQKSNPSLTSASVDEEEGDTCTICLEQWTNAGDHRLSTLRCGHLFGYKCISKWLRGQTRKCPQCNKKAKQSDIVVLYARTLRALDTSEQELMKSSLLKEQKLRKKAELDSAQCRLQLQALTDKCSRLQSRVQDLQKLIGQDKISQQPGGSQPHFLSGLPTSQSQQKYHFQKTFTVSQTGNCRVMTYCDALSCLVVSQPSPQSAFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSRSKGLLLSASLDSTIKLTSLETNTVVQTYNAGRPVWSCCWCLDENNYIYAGLVNGSILLYDLRNTSSHVQELVPQRARCPLVSLSYVPRAASAAFPYGGVLAGALENASFWELKMGFTHWPHVLPMEPGGCVDFQVESSTRHCLVTYRPDKNHNNLRSVLMEMSYKLDNAGEPICSCHPVQTFFGGPSCKLLTKNAIFQNPENNGSILVCIGDEASKSALLWDAGSGSLLQDLQADQPVLDICPFDVNHNSYLATLTEKTVHVYRWE

Target ORF information:

RefSeq Version	XM_005195170.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005195170.3

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC 
TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC 
CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA 
AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC 
TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG 
AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC 
ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG 
GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC 
CCCAAACAGT CTCCCCAGAA GTCTAACCCT TCTCTAACTT CTGCTTCTGT GGATGAGGAA 
GAAGGAGACA CTTGCACAAT ATGTTTGGAG CAGTGGACCA ATGCTGGGGA TCACCGGCTC 
TCTACATTGC GCTGTGGGCA CCTCTTTGGG TACAAATGCA TTTCGAAATG GCTCAGAGGA 
CAGACCCGAA AATGTCCCCA GTGTAACAAG AAAGCCAAGC AAAGTGATAT CGTTGTCCTT 
TATGCCCGGA CCTTGAGAGC TTTGGACACT AGTGAGCAGG AGCTCATGAA GAGTTCCTTA 
CTAAAGGAAC AGAAGCTAAG GAAGAAGGCT GAATTAGACT CGGCACAGTG TCGGCTGCAG 
CTTCAAGCCC TCACTGATAA ATGCTCTAGG CTTCAAAGTC GTGTTCAGGA CTTGCAGAAA 
CTTATCGGTC AGGATAAGAT CTCACAACAG CCTGGGGGTT CCCAACCACA TTTCCTAAGT 
GGCCTGCCCA CCAGCCAGAG TCAACAGAAG TACCATTTCC AGAAGACATT CACAGTGTCT 
CAAACAGGAA ACTGTCGAGT CATGACATAC TGTGATGCTT TGAGCTGCCT GGTGGTATCA 
CAACCTTCTC CTCAGTCCGC CTTCCTTCCA GGCTTTGGTG TTAAGATGTT GAGTACTGCC 
AACATGAAAA GCAGCCAGTA TATTCCTATG CATGGCAAAC AAATACGTGG ACTGGCTTTC 
AGCAGTCGAT CCAAAGGCTT ACTTCTCTCT GCTTCCCTGG ACAGCACAAT TAAACTGACC 
AGCCTGGAAA CAAATACCGT GGTCCAGACT TACAACGCTG GGCGGCCTGT CTGGAGCTGC 
TGCTGGTGTC TTGATGAAAA CAATTACATC TATGCTGGAC TGGTCAACGG TTCGATTCTG 
TTATATGACC TTCGAAATAC AAGCTCTCAC GTCCAGGAAT TAGTACCTCA GAGAGCTAGA 
TGCCCGCTGG TATCCCTGTC GTATGTGCCC AGAGCTGCCT CTGCTGCGTT TCCATATGGT 
GGCGTGTTGG CTGGAGCGTT GGAGAATGCC TCTTTTTGGG AGCTGAAAAT GGGTTTTACT 
CATTGGCCTC ATGTGCTGCC CATGGAGCCT GGGGGCTGCG TAGACTTTCA GGTAGAGAGC 
AGCACCCGCC ACTGTCTCGT GACCTATAGG CCTGATAAAA ATCACAACAA CTTACGAAGT 
GTACTCATGG AGATGTCCTA TAAACTGGAT AATGCTGGAG AGCCCATCTG CTCCTGCCAT 
CCTGTACAAA CATTCTTTGG GGGACCCTCC TGCAAATTGC TAACCAAGAA TGCCATTTTC 
CAAAATCCAG AGAATAATGG CAGCATCCTG GTGTGTATTG GGGATGAAGC ATCAAAATCT 
GCCCTGCTGT GGGATGCTGG CAGTGGCTCA TTGCTGCAGG ATCTGCAGGC CGATCAGCCT 
GTCTTGGACA TCTGCCCATT TGACGTGAAT CATAACAGCT ACTTGGCTAC CTTAACAGAA 
AAGACCGTCC ACGTCTATAG GTGGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_005218380.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218437.1 Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa09183
Clone ID Related Accession (Same CDS sequence)	XM_005195170.3 , XM_005218380.3 , XM_005218380.4
Accession Version	XM_005218380.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104462.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218380.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC 
TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC 
CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA 
AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC 
TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG 
AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC 
ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG 
GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC 
CCCAAACAGT CTCCCCAGAA GTCTAACCCT TCTCTAACTT CTGCTTCTGT GGATGAGGAA 
GAAGGAGACA CTTGCACAAT ATGTTTGGAG CAGTGGACCA ATGCTGGGGA TCACCGGCTC 
TCTACATTGC GCTGTGGGCA CCTCTTTGGG TACAAATGCA TTTCGAAATG GCTCAGAGGA 
CAGACCCGAA AATGTCCCCA GTGTAACAAG AAAGCCAAGC AAAGTGATAT CGTTGTCCTT 
TATGCCCGGA CCTTGAGAGC TTTGGACACT AGTGAGCAGG AGCTCATGAA GAGTTCCTTA 
CTAAAGGAAC AGAAGCTAAG GAAGAAGGCT GAATTAGACT CGGCACAGTG TCGGCTGCAG 
CTTCAAGCCC TCACTGATAA ATGCTCTAGG CTTCAAAGTC GTGTTCAGGA CTTGCAGAAA 
CTTATCGGTC AGGATAAGAT CTCACAACAG CCTGGGGGTT CCCAACCACA TTTCCTAAGT 
GGCCTGCCCA CCAGCCAGAG TCAACAGAAG TACCATTTCC AGAAGACATT CACAGTGTCT 
CAAACAGGAA ACTGTCGAGT CATGACATAC TGTGATGCTT TGAGCTGCCT GGTGGTATCA 
CAACCTTCTC CTCAGTCCGC CTTCCTTCCA GGCTTTGGTG TTAAGATGTT GAGTACTGCC 
AACATGAAAA GCAGCCAGTA TATTCCTATG CATGGCAAAC AAATACGTGG ACTGGCTTTC 
AGCAGTCGAT CCAAAGGCTT ACTTCTCTCT GCTTCCCTGG ACAGCACAAT TAAACTGACC 
AGCCTGGAAA CAAATACCGT GGTCCAGACT TACAACGCTG GGCGGCCTGT CTGGAGCTGC 
TGCTGGTGTC TTGATGAAAA CAATTACATC TATGCTGGAC TGGTCAACGG TTCGATTCTG 
TTATATGACC TTCGAAATAC AAGCTCTCAC GTCCAGGAAT TAGTACCTCA GAGAGCTAGA 
TGCCCGCTGG TATCCCTGTC GTATGTGCCC AGAGCTGCCT CTGCTGCGTT TCCATATGGT 
GGCGTGTTGG CTGGAGCGTT GGAGAATGCC TCTTTTTGGG AGCTGAAAAT GGGTTTTACT 
CATTGGCCTC ATGTGCTGCC CATGGAGCCT GGGGGCTGCG TAGACTTTCA GGTAGAGAGC 
AGCACCCGCC ACTGTCTCGT GACCTATAGG CCTGATAAAA ATCACAACAA CTTACGAAGT 
GTACTCATGG AGATGTCCTA TAAACTGGAT AATGCTGGAG AGCCCATCTG CTCCTGCCAT 
CCTGTACAAA CATTCTTTGG GGGACCCTCC TGCAAATTGC TAACCAAGAA TGCCATTTTC 
CAAAATCCAG AGAATAATGG CAGCATCCTG GTGTGTATTG GGGATGAAGC ATCAAAATCT 
GCCCTGCTGT GGGATGCTGG CAGTGGCTCA TTGCTGCAGG ATCTGCAGGC CGATCAGCCT 
GTCTTGGACA TCTGCCCATT TGACGTGAAT CATAACAGCT ACTTGGCTAC CTTAACAGAA 
AAGACCGTCC ACGTCTATAG GTGGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218437.1
CDS	140..2509
Translation	MAHEAMEYDLQVQLDHGAAEQPAPAEVVSSQGGPPLLQPPHTEEVSSQEGPPMLHPVLAEVVSIEGGPPLLQPIPEELINSPERVPLLPPIPQIPIDLEEVELSETETVGNLNPGVLEEFRPRSGANHTPQVTSLDSMNSFISGLQRLHGMLEFLRPTSDRRVGPVRARRSRRSSASRRARAGGSQRTNSARLRAPLDAFFQASRTQPHSSTTSYDSETRNLVSDDLQVSGSSDSDSDSSTEFEEGVAQAEEPEAVVLEEELEDTSTDQEVICAGGEDTVPKQSPQKSNPSLTSASVDEEEGDTCTICLEQWTNAGDHRLSTLRCGHLFGYKCISKWLRGQTRKCPQCNKKAKQSDIVVLYARTLRALDTSEQELMKSSLLKEQKLRKKAELDSAQCRLQLQALTDKCSRLQSRVQDLQKLIGQDKISQQPGGSQPHFLSGLPTSQSQQKYHFQKTFTVSQTGNCRVMTYCDALSCLVVSQPSPQSAFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSRSKGLLLSASLDSTIKLTSLETNTVVQTYNAGRPVWSCCWCLDENNYIYAGLVNGSILLYDLRNTSSHVQELVPQRARCPLVSLSYVPRAASAAFPYGGVLAGALENASFWELKMGFTHWPHVLPMEPGGCVDFQVESSTRHCLVTYRPDKNHNNLRSVLMEMSYKLDNAGEPICSCHPVQTFFGGPSCKLLTKNAIFQNPENNGSILVCIGDEASKSALLWDAGSGSLLQDLQADQPVLDICPFDVNHNSYLATLTEKTVHVYRWE

Target ORF information:

RefSeq Version	XM_005218380.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218380.3

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC 
TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC 
CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA 
AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC 
TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG 
AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC 
ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG 
GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC 
CCCAAACAGT CTCCCCAGAA GTCTAACCCT TCTCTAACTT CTGCTTCTGT GGATGAGGAA 
GAAGGAGACA CTTGCACAAT ATGTTTGGAG CAGTGGACCA ATGCTGGGGA TCACCGGCTC 
TCTACATTGC GCTGTGGGCA CCTCTTTGGG TACAAATGCA TTTCGAAATG GCTCAGAGGA 
CAGACCCGAA AATGTCCCCA GTGTAACAAG AAAGCCAAGC AAAGTGATAT CGTTGTCCTT 
TATGCCCGGA CCTTGAGAGC TTTGGACACT AGTGAGCAGG AGCTCATGAA GAGTTCCTTA 
CTAAAGGAAC AGAAGCTAAG GAAGAAGGCT GAATTAGACT CGGCACAGTG TCGGCTGCAG 
CTTCAAGCCC TCACTGATAA ATGCTCTAGG CTTCAAAGTC GTGTTCAGGA CTTGCAGAAA 
CTTATCGGTC AGGATAAGAT CTCACAACAG CCTGGGGGTT CCCAACCACA TTTCCTAAGT 
GGCCTGCCCA CCAGCCAGAG TCAACAGAAG TACCATTTCC AGAAGACATT CACAGTGTCT 
CAAACAGGAA ACTGTCGAGT CATGACATAC TGTGATGCTT TGAGCTGCCT GGTGGTATCA 
CAACCTTCTC CTCAGTCCGC CTTCCTTCCA GGCTTTGGTG TTAAGATGTT GAGTACTGCC 
AACATGAAAA GCAGCCAGTA TATTCCTATG CATGGCAAAC AAATACGTGG ACTGGCTTTC 
AGCAGTCGAT CCAAAGGCTT ACTTCTCTCT GCTTCCCTGG ACAGCACAAT TAAACTGACC 
AGCCTGGAAA CAAATACCGT GGTCCAGACT TACAACGCTG GGCGGCCTGT CTGGAGCTGC 
TGCTGGTGTC TTGATGAAAA CAATTACATC TATGCTGGAC TGGTCAACGG TTCGATTCTG 
TTATATGACC TTCGAAATAC AAGCTCTCAC GTCCAGGAAT TAGTACCTCA GAGAGCTAGA 
TGCCCGCTGG TATCCCTGTC GTATGTGCCC AGAGCTGCCT CTGCTGCGTT TCCATATGGT 
GGCGTGTTGG CTGGAGCGTT GGAGAATGCC TCTTTTTGGG AGCTGAAAAT GGGTTTTACT 
CATTGGCCTC ATGTGCTGCC CATGGAGCCT GGGGGCTGCG TAGACTTTCA GGTAGAGAGC 
AGCACCCGCC ACTGTCTCGT GACCTATAGG CCTGATAAAA ATCACAACAA CTTACGAAGT 
GTACTCATGG AGATGTCCTA TAAACTGGAT AATGCTGGAG AGCCCATCTG CTCCTGCCAT 
CCTGTACAAA CATTCTTTGG GGGACCCTCC TGCAAATTGC TAACCAAGAA TGCCATTTTC 
CAAAATCCAG AGAATAATGG CAGCATCCTG GTGTGTATTG GGGATGAAGC ATCAAAATCT 
GCCCTGCTGT GGGATGCTGG CAGTGGCTCA TTGCTGCAGG ATCTGCAGGC CGATCAGCCT 
GTCTTGGACA TCTGCCCATT TGACGTGAAT CATAACAGCT ACTTGGCTAC CTTAACAGAA 
AAGACCGTCC ACGTCTATAG GTGGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_005218380.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218437.1 Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

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CloneID	OBa09183
Clone ID Related Accession (Same CDS sequence)	XM_005195170.3 , XM_005218380.3 , XM_005218380.4
Accession Version	XM_005218380.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218380.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC 
TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC 
CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA 
AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC 
TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG 
AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC 
ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG 
GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC 
CCCAAACAGT CTCCCCAGAA GTCTAACCCT TCTCTAACTT CTGCTTCTGT GGATGAGGAA 
GAAGGAGACA CTTGCACAAT ATGTTTGGAG CAGTGGACCA ATGCTGGGGA TCACCGGCTC 
TCTACATTGC GCTGTGGGCA CCTCTTTGGG TACAAATGCA TTTCGAAATG GCTCAGAGGA 
CAGACCCGAA AATGTCCCCA GTGTAACAAG AAAGCCAAGC AAAGTGATAT CGTTGTCCTT 
TATGCCCGGA CCTTGAGAGC TTTGGACACT AGTGAGCAGG AGCTCATGAA GAGTTCCTTA 
CTAAAGGAAC AGAAGCTAAG GAAGAAGGCT GAATTAGACT CGGCACAGTG TCGGCTGCAG 
CTTCAAGCCC TCACTGATAA ATGCTCTAGG CTTCAAAGTC GTGTTCAGGA CTTGCAGAAA 
CTTATCGGTC AGGATAAGAT CTCACAACAG CCTGGGGGTT CCCAACCACA TTTCCTAAGT 
GGCCTGCCCA CCAGCCAGAG TCAACAGAAG TACCATTTCC AGAAGACATT CACAGTGTCT 
CAAACAGGAA ACTGTCGAGT CATGACATAC TGTGATGCTT TGAGCTGCCT GGTGGTATCA 
CAACCTTCTC CTCAGTCCGC CTTCCTTCCA GGCTTTGGTG TTAAGATGTT GAGTACTGCC 
AACATGAAAA GCAGCCAGTA TATTCCTATG CATGGCAAAC AAATACGTGG ACTGGCTTTC 
AGCAGTCGAT CCAAAGGCTT ACTTCTCTCT GCTTCCCTGG ACAGCACAAT TAAACTGACC 
AGCCTGGAAA CAAATACCGT GGTCCAGACT TACAACGCTG GGCGGCCTGT CTGGAGCTGC 
TGCTGGTGTC TTGATGAAAA CAATTACATC TATGCTGGAC TGGTCAACGG TTCGATTCTG 
TTATATGACC TTCGAAATAC AAGCTCTCAC GTCCAGGAAT TAGTACCTCA GAGAGCTAGA 
TGCCCGCTGG TATCCCTGTC GTATGTGCCC AGAGCTGCCT CTGCTGCGTT TCCATATGGT 
GGCGTGTTGG CTGGAGCGTT GGAGAATGCC TCTTTTTGGG AGCTGAAAAT GGGTTTTACT 
CATTGGCCTC ATGTGCTGCC CATGGAGCCT GGGGGCTGCG TAGACTTTCA GGTAGAGAGC 
AGCACCCGCC ACTGTCTCGT GACCTATAGG CCTGATAAAA ATCACAACAA CTTACGAAGT 
GTACTCATGG AGATGTCCTA TAAACTGGAT AATGCTGGAG AGCCCATCTG CTCCTGCCAT 
CCTGTACAAA CATTCTTTGG GGGACCCTCC TGCAAATTGC TAACCAAGAA TGCCATTTTC 
CAAAATCCAG AGAATAATGG CAGCATCCTG GTGTGTATTG GGGATGAAGC ATCAAAATCT 
GCCCTGCTGT GGGATGCTGG CAGTGGCTCA TTGCTGCAGG ATCTGCAGGC CGATCAGCCT 
GTCTTGGACA TCTGCCCATT TGACGTGAAT CATAACAGCT ACTTGGCTAC CTTAACAGAA 
AAGACCGTCC ACGTCTATAG GTGGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218437.1
CDS	157..2526
Translation	MAHEAMEYDLQVQLDHGAAEQPAPAEVVSSQGGPPLLQPPHTEEVSSQEGPPMLHPVLAEVVSIEGGPPLLQPIPEELINSPERVPLLPPIPQIPIDLEEVELSETETVGNLNPGVLEEFRPRSGANHTPQVTSLDSMNSFISGLQRLHGMLEFLRPTSDRRVGPVRARRSRRSSASRRARAGGSQRTNSARLRAPLDAFFQASRTQPHSSTTSYDSETRNLVSDDLQVSGSSDSDSDSSTEFEEGVAQAEEPEAVVLEEELEDTSTDQEVICAGGEDTVPKQSPQKSNPSLTSASVDEEEGDTCTICLEQWTNAGDHRLSTLRCGHLFGYKCISKWLRGQTRKCPQCNKKAKQSDIVVLYARTLRALDTSEQELMKSSLLKEQKLRKKAELDSAQCRLQLQALTDKCSRLQSRVQDLQKLIGQDKISQQPGGSQPHFLSGLPTSQSQQKYHFQKTFTVSQTGNCRVMTYCDALSCLVVSQPSPQSAFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSRSKGLLLSASLDSTIKLTSLETNTVVQTYNAGRPVWSCCWCLDENNYIYAGLVNGSILLYDLRNTSSHVQELVPQRARCPLVSLSYVPRAASAAFPYGGVLAGALENASFWELKMGFTHWPHVLPMEPGGCVDFQVESSTRHCLVTYRPDKNHNNLRSVLMEMSYKLDNAGEPICSCHPVQTFFGGPSCKLLTKNAIFQNPENNGSILVCIGDEASKSALLWDAGSGSLLQDLQADQPVLDICPFDVNHNSYLATLTEKTVHVYRWE

Target ORF information:

RefSeq Version	XM_005218380.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218380.4

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATCTT CAGGTGCAAT TAGATCATGG TGCTGCAGAA 
CAGCCTGCTC CTGCTGAGGT GGTCAGCAGC CAAGGGGGAC CACCCCTGCT CCAGCCTCCT 
CATACTGAGG AGGTCAGCAG CCAGGAGGGA CCACCCATGT TGCATCCTGT ACTTGCTGAG 
GTGGTCAGCA TCGAAGGAGG ACCACCTCTG CTCCAACCCA TTCCTGAGGA GCTGATCAAC 
AGCCCAGAGA GAGTGCCGCT GCTTCCGCCT ATTCCACAGA TACCTATTGA TCTAGAGGAA 
GTGGAGCTCT CAGAAACAGA GACTGTGGGG AACCTCAATC CAGGAGTTTT AGAGGAGTTT 
AGACCGAGAT CTGGTGCTAA CCACACTCCC CAAGTGACTT CATTGGATTC AATGAACAGC 
TTCATCAGTG GGCTGCAGAG ACTTCATGGC ATGCTGGAAT TCCTGCGACC AACTTCAGAC 
CGCAGAGTGG GGCCCGTGAG AGCGAGGAGG AGTAGGAGGA GTTCTGCTTC ACGGAGGGCA 
AGAGCAGGAG GGTCCCAGAG GACAAACAGT GCCAGGTTGA GAGCACCGTT GGATGCTTTC 
TTTCAAGCGA GCAGGACCCA GCCTCATTCA TCAACCACCT CTTATGATTC AGAGACTAGG 
AATCTTGTCT CTGATGACTT GCAGGTATCA GGTAGTTCTG ATTCTGACAG TGACAGCTCC 
ACAGAGTTTG AGGAGGGAGT TGCCCAGGCA GAGGAGCCTG AAGCTGTTGT TTTAGAAGAG 
GAACTAGAAG ACACCTCAAC AGACCAAGAA GTTATATGTG CTGGTGGAGA AGACACTGTC 
CCCAAACAGT CTCCCCAGAA GTCTAACCCT TCTCTAACTT CTGCTTCTGT GGATGAGGAA 
GAAGGAGACA CTTGCACAAT ATGTTTGGAG CAGTGGACCA ATGCTGGGGA TCACCGGCTC 
TCTACATTGC GCTGTGGGCA CCTCTTTGGG TACAAATGCA TTTCGAAATG GCTCAGAGGA 
CAGACCCGAA AATGTCCCCA GTGTAACAAG AAAGCCAAGC AAAGTGATAT CGTTGTCCTT 
TATGCCCGGA CCTTGAGAGC TTTGGACACT AGTGAGCAGG AGCTCATGAA GAGTTCCTTA 
CTAAAGGAAC AGAAGCTAAG GAAGAAGGCT GAATTAGACT CGGCACAGTG TCGGCTGCAG 
CTTCAAGCCC TCACTGATAA ATGCTCTAGG CTTCAAAGTC GTGTTCAGGA CTTGCAGAAA 
CTTATCGGTC AGGATAAGAT CTCACAACAG CCTGGGGGTT CCCAACCACA TTTCCTAAGT 
GGCCTGCCCA CCAGCCAGAG TCAACAGAAG TACCATTTCC AGAAGACATT CACAGTGTCT 
CAAACAGGAA ACTGTCGAGT CATGACATAC TGTGATGCTT TGAGCTGCCT GGTGGTATCA 
CAACCTTCTC CTCAGTCCGC CTTCCTTCCA GGCTTTGGTG TTAAGATGTT GAGTACTGCC 
AACATGAAAA GCAGCCAGTA TATTCCTATG CATGGCAAAC AAATACGTGG ACTGGCTTTC 
AGCAGTCGAT CCAAAGGCTT ACTTCTCTCT GCTTCCCTGG ACAGCACAAT TAAACTGACC 
AGCCTGGAAA CAAATACCGT GGTCCAGACT TACAACGCTG GGCGGCCTGT CTGGAGCTGC 
TGCTGGTGTC TTGATGAAAA CAATTACATC TATGCTGGAC TGGTCAACGG TTCGATTCTG 
TTATATGACC TTCGAAATAC AAGCTCTCAC GTCCAGGAAT TAGTACCTCA GAGAGCTAGA 
TGCCCGCTGG TATCCCTGTC GTATGTGCCC AGAGCTGCCT CTGCTGCGTT TCCATATGGT 
GGCGTGTTGG CTGGAGCGTT GGAGAATGCC TCTTTTTGGG AGCTGAAAAT GGGTTTTACT 
CATTGGCCTC ATGTGCTGCC CATGGAGCCT GGGGGCTGCG TAGACTTTCA GGTAGAGAGC 
AGCACCCGCC ACTGTCTCGT GACCTATAGG CCTGATAAAA ATCACAACAA CTTACGAAGT 
GTACTCATGG AGATGTCCTA TAAACTGGAT AATGCTGGAG AGCCCATCTG CTCCTGCCAT 
CCTGTACAAA CATTCTTTGG GGGACCCTCC TGCAAATTGC TAACCAAGAA TGCCATTTTC 
CAAAATCCAG AGAATAATGG CAGCATCCTG GTGTGTATTG GGGATGAAGC ATCAAAATCT 
GCCCTGCTGT GGGATGCTGG CAGTGGCTCA TTGCTGCAGG ATCTGCAGGC CGATCAGCCT 
GTCTTGGACA TCTGCCCATT TGACGTGAAT CATAACAGCT ACTTGGCTAC CTTAACAGAA 
AAGACCGTCC ACGTCTATAG GTGGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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RFWD3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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