ASNSD1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASNSD1
Entrez Gene ID	539672
Full Name	asparagine synthetase domain containing 1
General protein information	Preferred Names asparagine synthetase domain containing 1 Names asparagine synthetase domain-containing protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034680.2	NP_001029852.1	asparagine synthetase domain-containing protein 1
XM_005202206.3	XP_005202263.1	asparagine synthetase domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	YML096W	NP_013613.1
Magnaporthe oryzae (rice blast fungus)	MGG_05371	XP_003710217.1
Arabidopsis thaliana (thale cress)	AT2G03667	NP_001077875.2
Xenopus tropicalis (tropical clawed frog)	asnsd1	XP_002931571.1
Mus musculus (house mouse)	Asnsd1	NP_598489.2
	ASNSD1	XP_001106941.1
Kluyveromyces lactis	KLLA0D10285g	XP_453519.1
Oryza sativa (rice)	Os12g0574900	NP_001067104.1
Pan troglodytes (chimpanzee)	ASNSD1	XP_515983.3
Rattus norvegicus (Norway rat)	Asnsd1	NP_001100233.1
Danio rerio (zebrafish)	asnsd1	NP_956389.1
Schizosaccharomyces pombe (fission yeast)	SPBC4F6.11c	NP_596111.1
Homo sapiens (human)	ASNSD1	NP_061921.1
Canis lupus familiaris (dog)	ASNSD1	XP_005640497.1
Bos taurus (cattle)	ASNSD1	NP_001029852.1
Gallus gallus (chicken)	ASNSD1	NP_001006509.1
Drosophila melanogaster (fruit fly)	CG17486	NP_001036446.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010492	XP_001237560.2
Caenorhabditis elegans (roundworm)	M18.3	NP_502296.1
Eremothecium gossypii	AGOS_ADL350C	NP_983745.2
Neurospora crassa	NCU07300	XP_962154.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ASNSD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASNSD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa00149	NM_001034680.2 Latest version!	Bos taurus asparagine synthetase domain containing 1 (ASNSD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa16244	XM_005202206.3 Latest version!	Bos taurus asparagine synthetase domain containing 1 (ASNSD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASNSD1 ( NM_001034680.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001029852.1 Bos taurus asparagine synthetase domain containing 1 (ASNSD1), mRNA.

Price & Availability↑

CloneID	OBa00149
Clone ID Related Accession (Same CDS sequence)	NM_001034680.2
Accession Version	NM_001034680.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	asparagine synthetase domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC103382.1. On Aug 31, 2012 this sequence version replaced NM_001034680.1. ##Evidence-Data-START## Transcript exon combination :: BC103382.1, BT026275.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGTGTGGCA TTTGTTGTGC AGTAAGCTTT TCTGTTGAGC ATTTTAGCAG AGATTTGAAA 
GAGGATTTAC TGTGTAATCT TAAACGACGG GGGCCCGATA GTAGTAAACA GTTGTTAAGG 
TCTACTGTTA ACTACCAGTG TTTATTCTCT GGCCATGTCC TTCACTTAAG AGGTCTGTTG 
ACTGCCCAGC CTGTGGAAGA TGAAAGAGGC AATGTATTCC TGTGGAATGG AGAAATCTTT 
AGTGGGATCA AGGTTGAAGC CGAAGAGAAT GATACTCAAA TCATGTTTCA TTATCTTTCC 
TCTTGTAAGA ATGAATCTGA TATTTTGTCG CTCTTCTCAA AAGTCCAAGG TCCTTGGTCT 
TTTATATATT ATCAAGCATC TAGCCATTCT TTGTGGTTTG GTCGGGACTT TTTTGGTCGT 
CGTAGCTTGC TTTGGCATTT TAGTAACTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAGCAT CTGGAGTGAC TGATCAATGG CAGGAAGTTC CAGCATCTGG AATTTTCAGA 
ATTGATCTCA AGTCTGCTTC CATTTCCCAA TCTGTCGTTT TAAAGTTGTA TCCTTGGAAA 
TACAGTTCGG GGGGGGATGA TATCAGAGAA TGTGTTCATA ATAGCCTGAC TGAGATTTCA 
GCAGACTTGC CAACATTTGT CTTGGTGGCA GCAAATGAAG CCAAACTGTG TCTTAAAGAC 
CCTGTTGTCC CTTTAAATAT GGCGTTGCCA CAAGCTGCAT TTGAGACCCA TTGCAGCAGC 
ATTTCCAGGA GTCCACTCAC AAGAGAGACC CTTCGGGTCT TTCTTACAGA TGGACACACG 
AAGGAAGTAG TTCAGCAGTT CATTGGTGTC CTGAGCACCG CAGTCAAGAG ACGTGTCTTG 
TGTTTACCTA GGGATGAAAA CCTGGCACCA AGCGAAGTTT TGAAAACTAG TAACAGGAAA 
GCAAATGTTG CAGTTCTGTT TTCTGGAGGG ATTGATTCCA TGGTCATCGC AGCCCTTGCT 
GACCATCATA TTCCTTTAGA TGAACCAATC GATCTTCTTA ATGTGGCTTT CATGACTAAA 
GAAAAGACTA TACCAGTTAA TTTTAACAAA AAAGGGAGAA AACAGGCAAA TCATTGTGAA 
ATGCCCTCTG AAGAATTCTC CAAACATGCT GCTGCTACTG CTGCTGCCAG CCCTGGTGAA 
CAGTTGAGTG TGCCAGATCG AGTCACAGGA AGGGCGGGCC TGAAGGAACT GCAAGCTGCC 
AACCCTTCGC GGATTTGGAA TTTTGTTGAA ATTAATGTTT CTCTAGAAGA ACTGCAAAGA 
TTAAGAAGAA CTCGAATATC GCACTTAATC CAGCCCTTGG ATACAGTCCT GGATGATAGC 
ATTGGCTGTG CAGTCTGGTT TGCTTCCCGA GGAGCTGGCT GGTTAGTGAC CCAGGATGGA 
GCACAGCCGT ATCAGAGCAG TGCAAAGGTA GTTCTTACTG GAATTGGTGC AGATGAGCAG 
CTTGCAGGTT ATTCTCGTCA TCAGGTCCGC TTCCTGGCAC ATGGGCTGGA AGGACTGAAT 
AAGGAAATTG AAATGGAACT GGGTCGAATT TCTTCTAGAA ATCTCGGTCG TGATGATAGA 
GTTATCAGTG ATCATGGAAA AGAAGCAAGA TTTCCTTTCC TGGATGAAAA TGTTGTCTCC 
TTTCTAAATT CCCTACCAGT CTGGGAAAAG GCAAACTTGA CTTTACCCCG TGGAATTGGT 
GAAAAACTGA TTTTGCGTCT TGCAGCAGTG GAACTTGGTC TAACAGCCTC TGCTCTTCTG 
CCAAAGCGGG CCATGCAATT TGGATCCAGA ATTGCAAAAA TGGAAAAGAA TAATGAAAAG 
GCGTCTGATA AATGTGGAAG GCTCCAAATC ATCTCCTTAG AAAACCTTTC TGTTGAAAAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029852.1
CDS	386..2308
Translation	MCGICCAVSFSVEHFSRDLKEDLLCNLKRRGPDSSKQLLRSTVNYQCLFSGHVLHLRGLLTAQPVEDERGNVFLWNGEIFSGIKVEAEENDTQIMFHYLSSCKNESDILSLFSKVQGPWSFIYYQASSHSLWFGRDFFGRRSLLWHFSNLGKSFCLSSVGTQASGVTDQWQEVPASGIFRIDLKSASISQSVVLKLYPWKYSSGGDDIRECVHNSLTEISADLPTFVLVAANEAKLCLKDPVVPLNMALPQAAFETHCSSISRSPLTRETLRVFLTDGHTKEVVQQFIGVLSTAVKRRVLCLPRDENLAPSEVLKTSNRKANVAVLFSGGIDSMVIAALADHHIPLDEPIDLLNVAFMTKEKTIPVNFNKKGRKQANHCEMPSEEFSKHAAATAAASPGEQLSVPDRVTGRAGLKELQAANPSRIWNFVEINVSLEELQRLRRTRISHLIQPLDTVLDDSIGCAVWFASRGAGWLVTQDGAQPYQSSAKVVLTGIGADEQLAGYSRHQVRFLAHGLEGLNKEIEMELGRISSRNLGRDDRVISDHGKEARFPFLDENVVSFLNSLPVWEKANLTLPRGIGEKLILRLAAVELGLTASALLPKRAMQFGSRIAKMEKNNEKASDKCGRLQIISLENLSVEN

Target ORF information:

RefSeq Version	NM_001034680.2
Organism	Bos taurus(cattle)
Definition	Bos taurus asparagine synthetase domain containing 1 (ASNSD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034680.2

ORF Insert Sequence:

ATGTGTGGCA TTTGTTGTGC AGTAAGCTTT TCTGTTGAGC ATTTTAGCAG AGATTTGAAA 
GAGGATTTAC TGTGTAATCT TAAACGACGG GGGCCCGATA GTAGTAAACA GTTGTTAAGG 
TCTACTGTTA ACTACCAGTG TTTATTCTCT GGCCATGTCC TTCACTTAAG AGGTCTGTTG 
ACTGCCCAGC CTGTGGAAGA TGAAAGAGGC AATGTATTCC TGTGGAATGG AGAAATCTTT 
AGTGGGATCA AGGTTGAAGC CGAAGAGAAT GATACTCAAA TCATGTTTCA TTATCTTTCC 
TCTTGTAAGA ATGAATCTGA TATTTTGTCG CTCTTCTCAA AAGTCCAAGG TCCTTGGTCT 
TTTATATATT ATCAAGCATC TAGCCATTCT TTGTGGTTTG GTCGGGACTT TTTTGGTCGT 
CGTAGCTTGC TTTGGCATTT TAGTAACTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAGCAT CTGGAGTGAC TGATCAATGG CAGGAAGTTC CAGCATCTGG AATTTTCAGA 
ATTGATCTCA AGTCTGCTTC CATTTCCCAA TCTGTCGTTT TAAAGTTGTA TCCTTGGAAA 
TACAGTTCGG GGGGGGATGA TATCAGAGAA TGTGTTCATA ATAGCCTGAC TGAGATTTCA 
GCAGACTTGC CAACATTTGT CTTGGTGGCA GCAAATGAAG CCAAACTGTG TCTTAAAGAC 
CCTGTTGTCC CTTTAAATAT GGCGTTGCCA CAAGCTGCAT TTGAGACCCA TTGCAGCAGC 
ATTTCCAGGA GTCCACTCAC AAGAGAGACC CTTCGGGTCT TTCTTACAGA TGGACACACG 
AAGGAAGTAG TTCAGCAGTT CATTGGTGTC CTGAGCACCG CAGTCAAGAG ACGTGTCTTG 
TGTTTACCTA GGGATGAAAA CCTGGCACCA AGCGAAGTTT TGAAAACTAG TAACAGGAAA 
GCAAATGTTG CAGTTCTGTT TTCTGGAGGG ATTGATTCCA TGGTCATCGC AGCCCTTGCT 
GACCATCATA TTCCTTTAGA TGAACCAATC GATCTTCTTA ATGTGGCTTT CATGACTAAA 
GAAAAGACTA TACCAGTTAA TTTTAACAAA AAAGGGAGAA AACAGGCAAA TCATTGTGAA 
ATGCCCTCTG AAGAATTCTC CAAACATGCT GCTGCTACTG CTGCTGCCAG CCCTGGTGAA 
CAGTTGAGTG TGCCAGATCG AGTCACAGGA AGGGCGGGCC TGAAGGAACT GCAAGCTGCC 
AACCCTTCGC GGATTTGGAA TTTTGTTGAA ATTAATGTTT CTCTAGAAGA ACTGCAAAGA 
TTAAGAAGAA CTCGAATATC GCACTTAATC CAGCCCTTGG ATACAGTCCT GGATGATAGC 
ATTGGCTGTG CAGTCTGGTT TGCTTCCCGA GGAGCTGGCT GGTTAGTGAC CCAGGATGGA 
GCACAGCCGT ATCAGAGCAG TGCAAAGGTA GTTCTTACTG GAATTGGTGC AGATGAGCAG 
CTTGCAGGTT ATTCTCGTCA TCAGGTCCGC TTCCTGGCAC ATGGGCTGGA AGGACTGAAT 
AAGGAAATTG AAATGGAACT GGGTCGAATT TCTTCTAGAA ATCTCGGTCG TGATGATAGA 
GTTATCAGTG ATCATGGAAA AGAAGCAAGA TTTCCTTTCC TGGATGAAAA TGTTGTCTCC 
TTTCTAAATT CCCTACCAGT CTGGGAAAAG GCAAACTTGA CTTTACCCCG TGGAATTGGT 
GAAAAACTGA TTTTGCGTCT TGCAGCAGTG GAACTTGGTC TAACAGCCTC TGCTCTTCTG 
CCAAAGCGGG CCATGCAATT TGGATCCAGA ATTGCAAAAA TGGAAAAGAA TAATGAAAAG 
GCGTCTGATA AATGTGGAAG GCTCCAAATC ATCTCCTTAG AAAACCTTTC TGTTGAAAAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASNSD1 ( XM_005202206.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202263.1 Bos taurus asparagine synthetase domain containing 1 (ASNSD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa16244
Clone ID Related Accession (Same CDS sequence)	XM_005202206.3
Accession Version	XM_005202206.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	asparagine synthetase domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103830.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202206.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGGCA TTTGTTGTGC AGTAAGCTTT TCTGTTGAGC ATTTTAGCAG AGATTTGAAA 
GAGGATTTAC TGTGTAATCT TAAACGACGG GGGCCCGATA GTAGTAAACA GTTGTTAAGG 
TCTACTGTTA ACTACCAGTG TTTATTCTCT GGCCATGTCC TTCACTTAAG AGGTCTGTTG 
ACTGCCCAGC CTGTGGAAGA TGAAAGAGGC AATGTATTCC TGTGGAATGG AGAAGTCTTT 
AGTGGGATCA AGGTTGAAGC CGAAGAGAAT GATACTCAAA TCATGTTTCA TTATCTTTCC 
TCTTGTAAGA ATGAATCTGA TATTTTGTCG CTCTTCTCAG AAGTCCAAGG TCCTTGGTCT 
TTTATATATT ATCAAGCATC TAGCCATTCT TTGTGGTTTG GTCGGGACTT TTTTGGTCGT 
CGTAGCTTGC TTTGGCATTT TAGTAACTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAGCAT CTGGAGTGGC TGATCAATGG CAGGAAGTTC CAGCATCTGG AATTTTCAGA 
ATTGATCTCA AGTCTGCTTC CATTTCCCAA TCTGTCGTTT TAAAGTTGTA TCCTTGGAAA 
TACAGTTCGG GGGGGGATGA TATCAGAGAA TGTGTTCATA ATAGCCTGAC TGAGATTTCA 
GCAGACTTGC CAACATTAGT CTTGGTGGCA GCAAATGAAG CCAAACTGTA TCTTAAAGAC 
CCTGTTGTCC CTTTAAATAT GGCGTTGCCA CAAGCTGCAT TTGAGACCCA TTGCAGCAGC 
ATTTCCAGGA GTCCACTCAC AAGAGAGACC CTTCGGGTCT TTCTTACAGA TGGACACACG 
AAGGAAGTGG TTCAGCAGTT CATTGGTGTC CTGAGCACCG CGGTCAAGAG ACGTGTCTTG 
TGTTTACCTA GGGATGAAAA CCTGGCACCA AGCAAAGTTT TGAAAACTAG TAACGGGAAA 
GCAAATGTTG CAGTTCTGTT TTCTGGAGGG ATTGATTCCA TGGTCATCGC AGCCCTTGCT 
GACCATCATA TTCCTTTAGA TGAACCAATC GATCTTCTTA ATGTGGCTTT CATGACTAAA 
GAAAAGACTA TACCAGTTAA TTTTAACAAA AAAGGGAGAA AACAGGCAAA TCATTGTGAA 
ATGCCCTCTG AAGAATTCTC CAAACGTGCT GCTGCTACTG CTGCTGCCAG CCCTGGTGAA 
CAGTTGAGTG TGCCAGATCG AGTCACAGGA AGGGCGGGCC TGAAGGAACT GCAAGCTGCC 
AACCCTTCGC GGATTTGGAA TTTTGTTGAA ATTAATGTTT CTCTAGAAGA ACTGCAAAGA 
TTAAGAAGAA CTCGAATATC GCACTTAATC CAGCCCTTGG ATACAGTCCT GGATGATAGC 
ATTGGCTGTG CAGTCTGGTT TGCTTCCCGA GGAGCTGGCT GGTTAGTGAC CCAGGATGGA 
GCACAGCCGT ATCAGAGCAG TGCAAAGGTA GTTCTTACTG GAATTGGTGC AGATGAGCAG 
CTTGCAGGTT ATTCTCGTCA TCGGGTCCGC TTCCTGGCAC ATGGGCTGGA AGGACTGAAT 
AAGGAAATTG AAATGGAACT GGGTCGAATT TCTTCTAGAA ATCTCGGTCG TGATGATAGA 
GTTATCAGTG ATCATGGAAA AGAAGCAAGA TTTCCTTTCC TGGATGAAAA TGTTGTCTCC 
TTTCTAAATT CCCTACCAGT CTGGGAAAAG GCAAACTTGA CTTTACCCCG TGGAATTGGT 
GAAAAACTAA TTTTGCGTCT TGCAGCAGTG GAACTCGGTC TAACAGCCTC TGCTCTTCTG 
CCAAAGCGGG CCTTGCAATT TGGATCCAGA ATTGCAAAAA TGGAAAAGAA TAATGAAAAG 
GCGTCTGATA AATGTGGAAG GCTCCAAATC ATCTCCTTAG AAAACCTTTC TGTTGAAAAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202263.1
CDS	358..2280
Translation	MCGICCAVSFSVEHFSRDLKEDLLCNLKRRGPDSSKQLLRSTVNYQCLFSGHVLHLRGLLTAQPVEDERGNVFLWNGEVFSGIKVEAEENDTQIMFHYLSSCKNESDILSLFSEVQGPWSFIYYQASSHSLWFGRDFFGRRSLLWHFSNLGKSFCLSSVGTQASGVADQWQEVPASGIFRIDLKSASISQSVVLKLYPWKYSSGGDDIRECVHNSLTEISADLPTLVLVAANEAKLYLKDPVVPLNMALPQAAFETHCSSISRSPLTRETLRVFLTDGHTKEVVQQFIGVLSTAVKRRVLCLPRDENLAPSKVLKTSNGKANVAVLFSGGIDSMVIAALADHHIPLDEPIDLLNVAFMTKEKTIPVNFNKKGRKQANHCEMPSEEFSKRAAATAAASPGEQLSVPDRVTGRAGLKELQAANPSRIWNFVEINVSLEELQRLRRTRISHLIQPLDTVLDDSIGCAVWFASRGAGWLVTQDGAQPYQSSAKVVLTGIGADEQLAGYSRHRVRFLAHGLEGLNKEIEMELGRISSRNLGRDDRVISDHGKEARFPFLDENVVSFLNSLPVWEKANLTLPRGIGEKLILRLAAVELGLTASALLPKRALQFGSRIAKMEKNNEKASDKCGRLQIISLENLSVEN

Target ORF information:

RefSeq Version	XM_005202206.3
Organism	Bos taurus(cattle)
Definition	Bos taurus asparagine synthetase domain containing 1 (ASNSD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202206.3

ORF Insert Sequence:

ATGTGTGGCA TTTGTTGTGC AGTAAGCTTT TCTGTTGAGC ATTTTAGCAG AGATTTGAAA 
GAGGATTTAC TGTGTAATCT TAAACGACGG GGGCCCGATA GTAGTAAACA GTTGTTAAGG 
TCTACTGTTA ACTACCAGTG TTTATTCTCT GGCCATGTCC TTCACTTAAG AGGTCTGTTG 
ACTGCCCAGC CTGTGGAAGA TGAAAGAGGC AATGTATTCC TGTGGAATGG AGAAGTCTTT 
AGTGGGATCA AGGTTGAAGC CGAAGAGAAT GATACTCAAA TCATGTTTCA TTATCTTTCC 
TCTTGTAAGA ATGAATCTGA TATTTTGTCG CTCTTCTCAG AAGTCCAAGG TCCTTGGTCT 
TTTATATATT ATCAAGCATC TAGCCATTCT TTGTGGTTTG GTCGGGACTT TTTTGGTCGT 
CGTAGCTTGC TTTGGCATTT TAGTAACTTG GGCAAGAGTT TCTGCCTCTC TTCAGTTGGC 
ACCCAAGCAT CTGGAGTGGC TGATCAATGG CAGGAAGTTC CAGCATCTGG AATTTTCAGA 
ATTGATCTCA AGTCTGCTTC CATTTCCCAA TCTGTCGTTT TAAAGTTGTA TCCTTGGAAA 
TACAGTTCGG GGGGGGATGA TATCAGAGAA TGTGTTCATA ATAGCCTGAC TGAGATTTCA 
GCAGACTTGC CAACATTAGT CTTGGTGGCA GCAAATGAAG CCAAACTGTA TCTTAAAGAC 
CCTGTTGTCC CTTTAAATAT GGCGTTGCCA CAAGCTGCAT TTGAGACCCA TTGCAGCAGC 
ATTTCCAGGA GTCCACTCAC AAGAGAGACC CTTCGGGTCT TTCTTACAGA TGGACACACG 
AAGGAAGTGG TTCAGCAGTT CATTGGTGTC CTGAGCACCG CGGTCAAGAG ACGTGTCTTG 
TGTTTACCTA GGGATGAAAA CCTGGCACCA AGCAAAGTTT TGAAAACTAG TAACGGGAAA 
GCAAATGTTG CAGTTCTGTT TTCTGGAGGG ATTGATTCCA TGGTCATCGC AGCCCTTGCT 
GACCATCATA TTCCTTTAGA TGAACCAATC GATCTTCTTA ATGTGGCTTT CATGACTAAA 
GAAAAGACTA TACCAGTTAA TTTTAACAAA AAAGGGAGAA AACAGGCAAA TCATTGTGAA 
ATGCCCTCTG AAGAATTCTC CAAACGTGCT GCTGCTACTG CTGCTGCCAG CCCTGGTGAA 
CAGTTGAGTG TGCCAGATCG AGTCACAGGA AGGGCGGGCC TGAAGGAACT GCAAGCTGCC 
AACCCTTCGC GGATTTGGAA TTTTGTTGAA ATTAATGTTT CTCTAGAAGA ACTGCAAAGA 
TTAAGAAGAA CTCGAATATC GCACTTAATC CAGCCCTTGG ATACAGTCCT GGATGATAGC 
ATTGGCTGTG CAGTCTGGTT TGCTTCCCGA GGAGCTGGCT GGTTAGTGAC CCAGGATGGA 
GCACAGCCGT ATCAGAGCAG TGCAAAGGTA GTTCTTACTG GAATTGGTGC AGATGAGCAG 
CTTGCAGGTT ATTCTCGTCA TCGGGTCCGC TTCCTGGCAC ATGGGCTGGA AGGACTGAAT 
AAGGAAATTG AAATGGAACT GGGTCGAATT TCTTCTAGAA ATCTCGGTCG TGATGATAGA 
GTTATCAGTG ATCATGGAAA AGAAGCAAGA TTTCCTTTCC TGGATGAAAA TGTTGTCTCC 
TTTCTAAATT CCCTACCAGT CTGGGAAAAG GCAAACTTGA CTTTACCCCG TGGAATTGGT 
GAAAAACTAA TTTTGCGTCT TGCAGCAGTG GAACTCGGTC TAACAGCCTC TGCTCTTCTG 
CCAAAGCGGG CCTTGCAATT TGGATCCAGA ATTGCAAAAA TGGAAAAGAA TAATGAAAAG 
GCGTCTGATA AATGTGGAAG GCTCCAAATC ATCTCCTTAG AAAACCTTTC TGTTGAAAAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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ASNSD1 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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