GTPBP3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GTPBP3
Entrez Gene ID	539656
Full Name	GTP binding protein 3, mitochondrial
General protein information	Preferred Names GTP binding protein 3, mitochondrial Names tRNA modification GTPase GTPBP3, mitochondrial
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010806583.2	XP_010804885.1	tRNA modification GTPase GTPBP3, mitochondrial isoform X3
NM_001206218.2	NP_001193147.1	tRNA modification GTPase GTPBP3, mitochondrial
XM_010806582.2	XP_010804884.1	tRNA modification GTPase GTPBP3, mitochondrial isoform X1
XM_010806583.3	XP_010804885.1	tRNA modification GTPase GTPBP3, mitochondrial isoform X3
XM_005208516.3	XP_005208573.1	tRNA modification GTPase GTPBP3, mitochondrial isoform X2
XM_005208516.4	XP_005208573.1	tRNA modification GTPase GTPBP3, mitochondrial isoform X2
NM_001206218.1	NP_001193147.1	tRNA modification GTPase GTPBP3, mitochondrial
XM_010806582.3	XP_010804884.1	tRNA modification GTPase GTPBP3, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MSS1	NP_013736.1
Pan troglodytes (chimpanzee)	LOC748414	XP_001161204.3
Canis lupus familiaris (dog)	GTPBP3	XP_005632781.1
Mus musculus (house mouse)	Gtpbp3	NP_115933.2
Rattus norvegicus (Norway rat)	Gtpbp3	NP_001011919.1
Gallus gallus (chicken)	LOC100857447	XP_003643536.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002133	XP_320903.3
Schizosaccharomyces pombe (fission yeast)	mss1	NP_593144.1
Bos taurus (cattle)	GTPBP3	NP_001193147.1
Danio rerio (zebrafish)	gtpbp3	NP_001018605.1
Drosophila melanogaster (fruit fly)	CG18528	NP_651251.3
Kluyveromyces lactis	KLLA0E07657g	XP_454294.1
Eremothecium gossypii	AGOS_ABR063C	NP_983009.3
Magnaporthe oryzae (rice blast fungus)	MGG_04070	XP_003719769.1
Xenopus tropicalis (tropical clawed frog)	gtpbp3	XP_004911076.1
Homo sapiens (human)	GTPBP3	NP_598399.2
Caenorhabditis elegans (roundworm)	F39B2.7	NP_493573.1
Neurospora crassa	NCU04149	XP_961305.1
Arabidopsis thaliana (thale cress)	AT1G78010	NP_177924.3

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GTPBP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GTPBP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40061	NM_001206218.1 Latest version!	Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa20848	XM_005208516.3 Latest version!	Bos taurus GTP binding protein 3 (mitochondrial) (GTPBP3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa54413	XM_010806582.2 Latest version!	Bos taurus GTP binding protein 3 (mitochondrial) (GTPBP3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa54414	XM_010806583.2 Latest version!	Bos taurus GTP binding protein 3 (mitochondrial) (GTPBP3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa236192	XM_010806583.3 Latest version!	Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa239187	NM_001206218.2 Latest version!	Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa236190	XM_010806582.3 Latest version!	Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa236191	XM_005208516.4 Latest version!	Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GTPBP3 ( NM_001206218.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193147.1 Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), mRNA.

Price & Availability↑

CloneID	OBa40061
Clone ID Related Accession (Same CDS sequence)	NM_001206218.1
Accession Version	NM_001206218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	tRNA modification GTPase GTPBP3, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02019078.1. On Apr 20, 2011 this sequence version replaced XM_867558.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02822088, SAMN02822089 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTGGCGGG GGCTGTGGAC CCTGGTATCC CGGGCTGCAC GTGGGCCTCA CAGACTGTGC 
ACGCGCCAGG GCAGTGGTTA CCGGGCCCCC GGTTCCGGAG CCACTATCTT CGCTCTGAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCGGTG ATCCGAACCA GCGGCCCTGC CAGCGGCCAC 
GCCCTCCAGA GCCTCACGGC GCCGCGGGAC TTACCCCTGG CCCGCAAAGC CTGCCTGCGC 
CTGCTCAGCG ACCCCCGTTC TGGGGAGCCT TTGGACCGCG CGCTGGTGCT TTGGTTTCCA 
GGTCCCCAAA GTTTTACCGG TGAGGATTGC GCGGAGTTCC ACGTGCATGG AGGGCCAGCC 
GTGGTGAGTG GTGTCCTGCA GGCCCTGGGC AGTGTGCCAG GGCTGAGGCC AGCGGAAGCA 
GGCGAGTTCA CCAGGAGGGC GTTTGCCCAC GGGAAGCTGA GCCTGACGGA GGTGGAGGGG 
CTGGCAGACC TGATCCATGC AGAAACCGAG GCGCAGCGGC GACAGGCCCT GAGGCAGCTC 
GATGGGGAGC TAGGTGACCT CTGTCGCGGC TGGGCCGAGA CCCTCACTAA AGCTCTGGCC 
CATGTAGAGG CCTATATCGA TTTTAGTGAG GATGACAACC TGGAGGAAGG CGTCCTGGAT 
CAAGGTGGGT CCCCCTGGGG GTGGGAGGCG GAGACATCTG GCATCCAGCC CCCCCAGGTC 
CTCTTACTCT CTCTCTCCTT TCCTCCATCC ACAGCTGATA GTGAGGTGCG GAAGCTGGAG 
GTGGCGCTGG GCGTACATCT TCGAGATGCC AGGCGTGGGC AGAGGCTCCG CTCAGGGGCG 
CACGTGGTGG TTGCGGGACC TCCCAACGCC GGCAAAAGCA GCCTGGTGAA CCTGCTCAGC 
CGGAAGCCTG TGTCCATCGT GTCCCCGGAG CCGGGGACCA CCCGAGATGT TCTGGAGACC 
CCCGTGGACC TGGCCGGATT CCCAGCGCTG CTGAGCGACA CTGCGGGGTT GCGGGAAGGC 
GTGGGGCCTG TGGAGCAGGA GGGCGTGCGG CGTGCCCAAA AAAGGCTGGA GCAGGCTGAC 
CTCATTCTGG CCGTGTTGGA TGCTTCTGAC CTGGCCTCTC CGGCCAGCTG CAACTTCCTG 
GACACCGTTG TCATCCCGGC AGGAGCCAGG AACCCCAATG GGAGCCGCCA GCGCCTTCTG 
CTTGTGCTGA ATAAATCAGA CCTGCTGCCC GCTGGGCGCT CAGACCTCCA TCCTGACCTC 
CCCTCCCACC TGTTGCTGTC TTGCCTGACG GGTGAGGGGC TGGACGGCCT CCTAGAAGCA 
CTGAAGAAGG AGCTGGCTGC ACTGTGTGGG GACCCGTCCA CAGGCCCACT TCTGACACGG 
TCGAGGCACC AACACCACCT CCGGGGCTGC CTGGATGCCC TTGGTCAGTA CAAGCAGGCA 
AAAGACTTAG CCCTGGCAGC CGAGGCACTT CGGGTAGCCC GAGGGCATCT GAGTCATCTC 
ACTGGTGGAG GGGGCACTGA GGAAATTCTG GATATCATCT TCCGGGACTT CTGCGTGGGC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193147.1
CDS	1..1566
Translation	MWRGLWTLVSRAARGPHRLCTRQGSGYRAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALQSLTAPRDLPLARKACLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCAEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFAHGKLSLTEVEGLADLIHAETEAQRRQALRQLDGELGDLCRGWAETLTKALAHVEAYIDFSEDDNLEEGVLDQGGSPWGWEAETSGIQPPQVLLLSLSFPPSTADSEVRKLEVALGVHLRDARRGQRLRSGAHVVVAGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPALLSDTAGLREGVGPVEQEGVRRAQKRLEQADLILAVLDASDLASPASCNFLDTVVIPAGARNPNGSRQRLLLVLNKSDLLPAGRSDLHPDLPSHLLLSCLTGEGLDGLLEALKKELAALCGDPSTGPLLTRSRHQHHLRGCLDALGQYKQAKDLALAAEALRVARGHLSHLTGGGGTEEILDIIFRDFCVGK

Target ORF information:

RefSeq Version	NM_001206218.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206218.1

ORF Insert Sequence:

ATGTGGCGGG GGCTGTGGAC CCTGGTATCC CGGGCTGCAC GTGGGCCTCA CAGACTGTGC 
ACGCGCCAGG GCAGTGGTTA CCGGGCCCCC GGTTCCGGAG CCACTATCTT CGCTCTGAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCGGTG ATCCGAACCA GCGGCCCTGC CAGCGGCCAC 
GCCCTCCAGA GCCTCACGGC GCCGCGGGAC TTACCCCTGG CCCGCAAAGC CTGCCTGCGC 
CTGCTCAGCG ACCCCCGTTC TGGGGAGCCT TTGGACCGCG CGCTGGTGCT TTGGTTTCCA 
GGTCCCCAAA GTTTTACCGG TGAGGATTGC GCGGAGTTCC ACGTGCATGG AGGGCCAGCC 
GTGGTGAGTG GTGTCCTGCA GGCCCTGGGC AGTGTGCCAG GGCTGAGGCC AGCGGAAGCA 
GGCGAGTTCA CCAGGAGGGC GTTTGCCCAC GGGAAGCTGA GCCTGACGGA GGTGGAGGGG 
CTGGCAGACC TGATCCATGC AGAAACCGAG GCGCAGCGGC GACAGGCCCT GAGGCAGCTC 
GATGGGGAGC TAGGTGACCT CTGTCGCGGC TGGGCCGAGA CCCTCACTAA AGCTCTGGCC 
CATGTAGAGG CCTATATCGA TTTTAGTGAG GATGACAACC TGGAGGAAGG CGTCCTGGAT 
CAAGGTGGGT CCCCCTGGGG GTGGGAGGCG GAGACATCTG GCATCCAGCC CCCCCAGGTC 
CTCTTACTCT CTCTCTCCTT TCCTCCATCC ACAGCTGATA GTGAGGTGCG GAAGCTGGAG 
GTGGCGCTGG GCGTACATCT TCGAGATGCC AGGCGTGGGC AGAGGCTCCG CTCAGGGGCG 
CACGTGGTGG TTGCGGGACC TCCCAACGCC GGCAAAAGCA GCCTGGTGAA CCTGCTCAGC 
CGGAAGCCTG TGTCCATCGT GTCCCCGGAG CCGGGGACCA CCCGAGATGT TCTGGAGACC 
CCCGTGGACC TGGCCGGATT CCCAGCGCTG CTGAGCGACA CTGCGGGGTT GCGGGAAGGC 
GTGGGGCCTG TGGAGCAGGA GGGCGTGCGG CGTGCCCAAA AAAGGCTGGA GCAGGCTGAC 
CTCATTCTGG CCGTGTTGGA TGCTTCTGAC CTGGCCTCTC CGGCCAGCTG CAACTTCCTG 
GACACCGTTG TCATCCCGGC AGGAGCCAGG AACCCCAATG GGAGCCGCCA GCGCCTTCTG 
CTTGTGCTGA ATAAATCAGA CCTGCTGCCC GCTGGGCGCT CAGACCTCCA TCCTGACCTC 
CCCTCCCACC TGTTGCTGTC TTGCCTGACG GGTGAGGGGC TGGACGGCCT CCTAGAAGCA 
CTGAAGAAGG AGCTGGCTGC ACTGTGTGGG GACCCGTCCA CAGGCCCACT TCTGACACGG 
TCGAGGCACC AACACCACCT CCGGGGCTGC CTGGATGCCC TTGGTCAGTA CAAGCAGGCA 
AAAGACTTAG CCCTGGCAGC CGAGGCACTT CGGGTAGCCC GAGGGCATCT GAGTCATCTC 
ACTGGTGGAG GGGGCACTGA GGAAATTCTG GATATCATCT TCCGGGACTT CTGCGTGGGC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTPBP3 ( XM_005208516.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208573.1 Bos taurus GTP binding protein 3 (mitochondrial) (GTPBP3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa20848
Clone ID Related Accession (Same CDS sequence)	XM_005208516.3
Accession Version	XM_005208516.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tRNA modification GTPase GTPBP3, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208516.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGG AGGAACACCA TTTCACTGCA CCGCAGCGTG CGAGCCCTCG GCTTCTTTCC 
CTGTCATTAG CCAGTAGAAG CGAAGATCTT CAGTGGATGG GCGGTACGTT TTCTACGGGG 
CCCAATGATA ATCGAGAATT GTTGAGCACT TTACCAGTCA CGCCGCGGAG TGGGCGGAAC 
CTCCTGCGAG GACTTGCAGA CCCCCGCGGC AGAGTGGGCA GGCGGGGAGC TGATTGTGAA 
TCCATGTGGC GGGGGCTGTG GACCCTGGTA TCCCGGGCTG CACGTGGGCC TCACAGTCCC 
AGACTGTGCA CGCGCCAGGG CAGTGGTTAC CGGGCCCCCG GTTCCGGAGC CACTATCTTC 
GCTCTGAGCT CTGGCCAAGG CCGCTGCGGC ATCGCGGTGA TCCGAACCAG CGGCCCTGCC 
AGCGGCCACG CCCTCCAGAG CCTCACGGCG CCGCGGGACT TACCCCTGGC CCGCAAAGCC 
TGCCTGCGCC TGCTCAGCGA CCCCCGTTCT GGGGAGCCTT TGGACCGCGC GCTGGTGCTT 
TGGTTTCCAG GTCCCCAAAG TTTTACCGGT GAGGATTGCG CGGAGTTCCA CGTGCATGGA 
GGGCCAGCCG TGGTGAGTGG TGTCCTGCAG GCCCTGGGCA GTGTGCCAGG GCTGAGGCCA 
GCGGAAGCAG GCGAGTTCAC CAGGAGGGCG TTTGCCCACG GGAAGCTGAG CCTGACGGAG 
GTGGAGGGGC TGGCAGACCT GATCCATGCA GAAACCGAGG CGCAGCGGCG ACAGGCCCTG 
AGGCAGCTCG ATGGGGAGCT AGGTGACCTC TGTCGCGGCT GGGCCGAGAC CCTCACTAAA 
GCTCTGGCCC ATGTAGAGGC CTATATCGAT TTTAGTGAGG ATGACAACCT GGAGGAAGGC 
GTCCTGGATC AAGCTGATAG TGAGGTGCGG AAGCTGGAGG TGGCGCTGGG CGTACATCTT 
CGAGATGCCA GGCGTGGGCA GAGGCTCCGC TCAGGGGCGC ACGTGGTGGT TGCGGGACCT 
CCCAACGCCG GCAAAAGCAG CCTGGTGAAC CTGCTCAGCC GGAAGCCTGT GTCCATCGTG 
TCCCCGGAGC CGGGGACCAC CCGAGATGTT CTGGAGACCC CCGTGGACCT GGCCGGATTC 
CCAGCGCTGC TGAGCGACAC TGCGGGGTTG CGGGAAGGCG TGGGGCCTGT GGAGCAGGAG 
GGCGTGCGGC GTGCCCAAAA AAGGCTGGAG CAGGCTGACC TCATTCTGGC CGTGTTGGAT 
GCTTCTGACC TGGCCTCTCC GGCCAGCTGC AACTTCCTGG ACACCGTTGT CATCCCGGCA 
GGAGCCAGGA ACCCCAATGG GAGCCGCCAG CGCCTTCTGC TTGTGCTGAA TAAATCAGAC 
CTGCTGCCCG CTGGGCGCTC AGACCTCCAT CCTGACCTCC CCTCCCACCT GTTGCTGTCT 
TGCCTGACGG GTGAGGGGCT GGACGGCCTC CTAGAAGCAC TGAAGAAGGA GCTGGCTGCA 
CTGTGTGGGG ACCCGTCCAC AGGCCCACTT CTGACACGGT CGAGGCACCA ACACCACCTC 
CGGGGCTGCC TGGATGCCCT TGGTCAGTAC AAGCAGGCAA AAGACTTAGC CCTGGCAGCC 
GAGGCACTTC GGGTAGCCCG AGGGCATCTG AGTCATCTCA CTGGTGGAGG GGGCACTGAG 
GAAATTCTGG ATATCATCTT CCGGGACTTC TGCGTGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208573.1
CDS	826..2550
Translation	MLLEEHHFTAPQRASPRLLSLSLASRSEDLQWMGGTFSTGPNDNRELLSTLPVTPRSGRNLLRGLADPRGRVGRRGADCESMWRGLWTLVSRAARGPHSPRLCTRQGSGYRAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALQSLTAPRDLPLARKACLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCAEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFAHGKLSLTEVEGLADLIHAETEAQRRQALRQLDGELGDLCRGWAETLTKALAHVEAYIDFSEDDNLEEGVLDQADSEVRKLEVALGVHLRDARRGQRLRSGAHVVVAGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPALLSDTAGLREGVGPVEQEGVRRAQKRLEQADLILAVLDASDLASPASCNFLDTVVIPAGARNPNGSRQRLLLVLNKSDLLPAGRSDLHPDLPSHLLLSCLTGEGLDGLLEALKKELAALCGDPSTGPLLTRSRHQHHLRGCLDALGQYKQAKDLALAAEALRVARGHLSHLTGGGGTEEILDIIFRDFCVGK

Target ORF information:

RefSeq Version	XM_005208516.3
Organism	Bos taurus(cattle)
Definition	Bos taurus GTP binding protein 3 (mitochondrial) (GTPBP3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208516.3

ORF Insert Sequence:

ATGTTGCTGG AGGAACACCA TTTCACTGCA CCGCAGCGTG CGAGCCCTCG GCTTCTTTCC 
CTGTCATTAG CCAGTAGAAG CGAAGATCTT CAGTGGATGG GCGGTACGTT TTCTACGGGG 
CCCAATGATA ATCGAGAATT GTTGAGCACT TTACCAGTCA CGCCGCGGAG TGGGCGGAAC 
CTCCTGCGAG GACTTGCAGA CCCCCGCGGC AGAGTGGGCA GGCGGGGAGC TGATTGTGAA 
TCCATGTGGC GGGGGCTGTG GACCCTGGTA TCCCGGGCTG CACGTGGGCC TCACAGTCCC 
AGACTGTGCA CGCGCCAGGG CAGTGGTTAC CGGGCCCCCG GTTCCGGAGC CACTATCTTC 
GCTCTGAGCT CTGGCCAAGG CCGCTGCGGC ATCGCGGTGA TCCGAACCAG CGGCCCTGCC 
AGCGGCCACG CCCTCCAGAG CCTCACGGCG CCGCGGGACT TACCCCTGGC CCGCAAAGCC 
TGCCTGCGCC TGCTCAGCGA CCCCCGTTCT GGGGAGCCTT TGGACCGCGC GCTGGTGCTT 
TGGTTTCCAG GTCCCCAAAG TTTTACCGGT GAGGATTGCG CGGAGTTCCA CGTGCATGGA 
GGGCCAGCCG TGGTGAGTGG TGTCCTGCAG GCCCTGGGCA GTGTGCCAGG GCTGAGGCCA 
GCGGAAGCAG GCGAGTTCAC CAGGAGGGCG TTTGCCCACG GGAAGCTGAG CCTGACGGAG 
GTGGAGGGGC TGGCAGACCT GATCCATGCA GAAACCGAGG CGCAGCGGCG ACAGGCCCTG 
AGGCAGCTCG ATGGGGAGCT AGGTGACCTC TGTCGCGGCT GGGCCGAGAC CCTCACTAAA 
GCTCTGGCCC ATGTAGAGGC CTATATCGAT TTTAGTGAGG ATGACAACCT GGAGGAAGGC 
GTCCTGGATC AAGCTGATAG TGAGGTGCGG AAGCTGGAGG TGGCGCTGGG CGTACATCTT 
CGAGATGCCA GGCGTGGGCA GAGGCTCCGC TCAGGGGCGC ACGTGGTGGT TGCGGGACCT 
CCCAACGCCG GCAAAAGCAG CCTGGTGAAC CTGCTCAGCC GGAAGCCTGT GTCCATCGTG 
TCCCCGGAGC CGGGGACCAC CCGAGATGTT CTGGAGACCC CCGTGGACCT GGCCGGATTC 
CCAGCGCTGC TGAGCGACAC TGCGGGGTTG CGGGAAGGCG TGGGGCCTGT GGAGCAGGAG 
GGCGTGCGGC GTGCCCAAAA AAGGCTGGAG CAGGCTGACC TCATTCTGGC CGTGTTGGAT 
GCTTCTGACC TGGCCTCTCC GGCCAGCTGC AACTTCCTGG ACACCGTTGT CATCCCGGCA 
GGAGCCAGGA ACCCCAATGG GAGCCGCCAG CGCCTTCTGC TTGTGCTGAA TAAATCAGAC 
CTGCTGCCCG CTGGGCGCTC AGACCTCCAT CCTGACCTCC CCTCCCACCT GTTGCTGTCT 
TGCCTGACGG GTGAGGGGCT GGACGGCCTC CTAGAAGCAC TGAAGAAGGA GCTGGCTGCA 
CTGTGTGGGG ACCCGTCCAC AGGCCCACTT CTGACACGGT CGAGGCACCA ACACCACCTC 
CGGGGCTGCC TGGATGCCCT TGGTCAGTAC AAGCAGGCAA AAGACTTAGC CCTGGCAGCC 
GAGGCACTTC GGGTAGCCCG AGGGCATCTG AGTCATCTCA CTGGTGGAGG GGGCACTGAG 
GAAATTCTGG ATATCATCTT CCGGGACTTC TGCGTGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTPBP3 ( XM_010806582.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804884.1 Bos taurus GTP binding protein 3 (mitochondrial) (GTPBP3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa54413
Clone ID Related Accession (Same CDS sequence)	XM_010806582.2
Accession Version	XM_010806582.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tRNA modification GTPase GTPBP3, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104002.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806582.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGG AGGAACACCA TTTCACTGCA CCGCAGCGTG CGAGCCCTCG GCTTCTTTCC 
CTGTCATTAG CCAGTAGAAG CGAAGATCTT CAGTGGATGG GCGGTACGTT TTCTACGGGG 
CCCAATGATA ATCGAGAATT GTTGAGCACT TTACCAGTCA CGCCGCGGAG TGGGCGGAAC 
CTCCTGCGAG GACTTGCAGA CCCCCGCGGC AGAGTGGGCA GGCGGGGAGC TGATTGTGAA 
TCCATGTGGC GGGGGCTGTG GACCCTGGTA TCCCGGGCTG CACGTGGGCC TCACAGTCCC 
AGACTGTGCA CGCGCCAGGG CAGTGGTTAC CGGGCCCCCG GTTCCGGAGC CACTATCTTC 
GCTCTGAGCT CTGGCCAAGG CCGCTGCGGC ATCGCGGTGA TCCGAACCAG CGGCCCTGCC 
AGCGGCCACG CCCTCCAGAG CCTCACGGCG CCGCGGGACT TACCCCTGGC CCGCAAAGCC 
TGCCTGCGCC TGCTCAGCGA CCCCCGTTCT GGGGAGCCTT TGGACCGCGC GCTGGTGCTT 
TGGTTTCCAG GTCCCCAAAG TTTTACCGGT GAGGATTGCG CGGAGTTCCA CGTGCATGGA 
GGGCCAGCCG TGGTGAGTGG TGTCCTGCAG GCCCTGGGCA GTGTGCCAGG GCTGAGGCCA 
GCGGAAGCAG GCGAGTTCAC CAGGAGGGCG TTTGCCCACG GGAAGCTGAG CCTGACGGAG 
GTGGAGGGGC TGGCAGACCT GATCCATGCA GAAACCGAGG CGCAGCGGCG ACAGGCCCTG 
AGGCAGCTCG ATGGGGAGCT AGGTGACCTC TGTCGCGGCT GGGCCGAGAC CCTCACTAAA 
GCTCTGGCCC ATGTAGAGGC CTATATCGAT TTTAGTGAGG ATGACAACCT GGAGGAAGGC 
GTCCTGGATC AAGGTGGGTC CCCCTGGGGG TGGGAGGCGG AGACATCTGG CATCCAGCCC 
CCCCAGGTCC TCTTACTCTC TCTCTCCTTT CCTCCATCCA CAGCTGATAG TGAGGTGCGG 
AAGCTGGAGG TGGCGCTGGG CGTACATCTT CGAGATGCCA GGCGTGGGCA GAGGCTCCGC 
TCAGGGGCGC ACGTGGTGGT TGCGGGACCT CCCAACGCCG GCAAAAGCAG CCTGGTGAAC 
CTGCTCAGCC GGAAGCCTGT GTCCATCGTG TCCCCGGAGC CGGGGACCAC CCGAGATGTT 
CTGGAGACCC CCGTGGACCT GGCCGGATTC CCAGCGCTGC TGAGCGACAC TGCGGGGTTG 
CGGGAAGGCG TGGGGCCTGT GGAGCAGGAG GGCGTGCGGC GTGCCCAAAA AAGGCTGGAG 
CAGGCTGACC TCATTCTGGC CGTGTTGGAT GCTTCTGACC TGGCCTCTCC GGCCAGCTGC 
AACTTCCTGG ACACCGTTGT CATCCCGGCA GGAGCCAGGA ACCCCAATGG GAGCCGCCAG 
CGCCTTCTGC TTGTGCTGAA TAAATCAGAC CTGCTGCCCG CTGGGCGCTC AGACCTCCAT 
CCTGACCTCC CCTCCCACCT GTTGCTGTCT TGCCTGACGG GTGAGGGGCT GGACGGCCTC 
CTAGAAGCAC TGAAGAAGGA GCTGGCTGCA CTGTGTGGGG ACCCGTCCAC AGGCCCACTT 
CTGACACGGT CGAGGCACCA ACACCACCTC CGGGGCTGCC TGGATGCCCT TGGTCAGTAC 
AAGCAGGCAA AAGACTTAGC CCTGGCAGCC GAGGCACTTC GGGTAGCCCG AGGGCATCTG 
AGTCATCTCA CTGGTGGAGG GGGCACTGAG GAAATTCTGG ATATCATCTT CCGGGACTTC 
TGCGTGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804884.1
CDS	827..2641
Translation	MLLEEHHFTAPQRASPRLLSLSLASRSEDLQWMGGTFSTGPNDNRELLSTLPVTPRSGRNLLRGLADPRGRVGRRGADCESMWRGLWTLVSRAARGPHSPRLCTRQGSGYRAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALQSLTAPRDLPLARKACLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCAEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFAHGKLSLTEVEGLADLIHAETEAQRRQALRQLDGELGDLCRGWAETLTKALAHVEAYIDFSEDDNLEEGVLDQGGSPWGWEAETSGIQPPQVLLLSLSFPPSTADSEVRKLEVALGVHLRDARRGQRLRSGAHVVVAGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPALLSDTAGLREGVGPVEQEGVRRAQKRLEQADLILAVLDASDLASPASCNFLDTVVIPAGARNPNGSRQRLLLVLNKSDLLPAGRSDLHPDLPSHLLLSCLTGEGLDGLLEALKKELAALCGDPSTGPLLTRSRHQHHLRGCLDALGQYKQAKDLALAAEALRVARGHLSHLTGGGGTEEILDIIFRDFCVGK

Target ORF information:

RefSeq Version	XM_010806582.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GTP binding protein 3 (mitochondrial) (GTPBP3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010806582.2

ORF Insert Sequence:

ATGTTGCTGG AGGAACACCA TTTCACTGCA CCGCAGCGTG CGAGCCCTCG GCTTCTTTCC 
CTGTCATTAG CCAGTAGAAG CGAAGATCTT CAGTGGATGG GCGGTACGTT TTCTACGGGG 
CCCAATGATA ATCGAGAATT GTTGAGCACT TTACCAGTCA CGCCGCGGAG TGGGCGGAAC 
CTCCTGCGAG GACTTGCAGA CCCCCGCGGC AGAGTGGGCA GGCGGGGAGC TGATTGTGAA 
TCCATGTGGC GGGGGCTGTG GACCCTGGTA TCCCGGGCTG CACGTGGGCC TCACAGTCCC 
AGACTGTGCA CGCGCCAGGG CAGTGGTTAC CGGGCCCCCG GTTCCGGAGC CACTATCTTC 
GCTCTGAGCT CTGGCCAAGG CCGCTGCGGC ATCGCGGTGA TCCGAACCAG CGGCCCTGCC 
AGCGGCCACG CCCTCCAGAG CCTCACGGCG CCGCGGGACT TACCCCTGGC CCGCAAAGCC 
TGCCTGCGCC TGCTCAGCGA CCCCCGTTCT GGGGAGCCTT TGGACCGCGC GCTGGTGCTT 
TGGTTTCCAG GTCCCCAAAG TTTTACCGGT GAGGATTGCG CGGAGTTCCA CGTGCATGGA 
GGGCCAGCCG TGGTGAGTGG TGTCCTGCAG GCCCTGGGCA GTGTGCCAGG GCTGAGGCCA 
GCGGAAGCAG GCGAGTTCAC CAGGAGGGCG TTTGCCCACG GGAAGCTGAG CCTGACGGAG 
GTGGAGGGGC TGGCAGACCT GATCCATGCA GAAACCGAGG CGCAGCGGCG ACAGGCCCTG 
AGGCAGCTCG ATGGGGAGCT AGGTGACCTC TGTCGCGGCT GGGCCGAGAC CCTCACTAAA 
GCTCTGGCCC ATGTAGAGGC CTATATCGAT TTTAGTGAGG ATGACAACCT GGAGGAAGGC 
GTCCTGGATC AAGGTGGGTC CCCCTGGGGG TGGGAGGCGG AGACATCTGG CATCCAGCCC 
CCCCAGGTCC TCTTACTCTC TCTCTCCTTT CCTCCATCCA CAGCTGATAG TGAGGTGCGG 
AAGCTGGAGG TGGCGCTGGG CGTACATCTT CGAGATGCCA GGCGTGGGCA GAGGCTCCGC 
TCAGGGGCGC ACGTGGTGGT TGCGGGACCT CCCAACGCCG GCAAAAGCAG CCTGGTGAAC 
CTGCTCAGCC GGAAGCCTGT GTCCATCGTG TCCCCGGAGC CGGGGACCAC CCGAGATGTT 
CTGGAGACCC CCGTGGACCT GGCCGGATTC CCAGCGCTGC TGAGCGACAC TGCGGGGTTG 
CGGGAAGGCG TGGGGCCTGT GGAGCAGGAG GGCGTGCGGC GTGCCCAAAA AAGGCTGGAG 
CAGGCTGACC TCATTCTGGC CGTGTTGGAT GCTTCTGACC TGGCCTCTCC GGCCAGCTGC 
AACTTCCTGG ACACCGTTGT CATCCCGGCA GGAGCCAGGA ACCCCAATGG GAGCCGCCAG 
CGCCTTCTGC TTGTGCTGAA TAAATCAGAC CTGCTGCCCG CTGGGCGCTC AGACCTCCAT 
CCTGACCTCC CCTCCCACCT GTTGCTGTCT TGCCTGACGG GTGAGGGGCT GGACGGCCTC 
CTAGAAGCAC TGAAGAAGGA GCTGGCTGCA CTGTGTGGGG ACCCGTCCAC AGGCCCACTT 
CTGACACGGT CGAGGCACCA ACACCACCTC CGGGGCTGCC TGGATGCCCT TGGTCAGTAC 
AAGCAGGCAA AAGACTTAGC CCTGGCAGCC GAGGCACTTC GGGTAGCCCG AGGGCATCTG 
AGTCATCTCA CTGGTGGAGG GGGCACTGAG GAAATTCTGG ATATCATCTT CCGGGACTTC 
TGCGTGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTPBP3 ( XM_010806583.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804885.1 Bos taurus GTP binding protein 3 (mitochondrial) (GTPBP3), transcript variant X3, mRNA.

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CloneID	OBa54414
Clone ID Related Accession (Same CDS sequence)	XM_010806583.2
Accession Version	XM_010806583.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tRNA modification GTPase GTPBP3, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806583.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGG AGGAACACCA TTTCACTGCA CCGCAGCGTG CGAGCCCTCG GCTTCTTTCC 
CTGTCATTAG CCAGTAGAAG CGAAGATCTT CAGTGGATGG GCGGTACGTT TTCTACGGGG 
CCCAATGATA ATCGAGAATT GTTGAGCACT TTACCAGTCA CGCCGCGGAG TGGGCGGAAC 
CTCCTGCGAG GACTTGCAGA CCCCCGCGGC AGAGTGGGCA GGCGGGGAGC TGATTGTGAA 
TCCATGTGGC GGGGGCTGTG GACCCTGGTA TCCCGGGCTG CACGTGGGCC TCACAGTCCC 
AGACTGTGCA CGCGCCAGGG CAGTGGTTAC CGGGCCCCCG GTTCCGGAGC CACTATCTTC 
GCTCTGAGCT CTGGCCAAGG CCGCTGCGGC ATCGCGGTGA TCCGAACCAG CGGCCCTGCC 
AGCGGCCACG CCCTCCAGAG CCTCACGGCG CCGCGGGACT TACCCCTGGC CCGCAAAGCC 
TGCCTGCGCC TGCTCAGCGA CCCCCGTTCT GGGGAGCCTT TGGACCGCGC GCTGGTGCTT 
TGGTTTCCAG GTCCCCAAAG TTTTACCGGT GAGGATTGCG CGGAGTTCCA CGTGCATGGA 
GGGCCAGCCG TGGTGAGTGG TGTCCTGCAG GCCCTGGGCA GTGTGCCAGG GCTGAGGCCA 
GCGGAAGCAG GCGAGTTCAC CAGGAGGGCG TTTGCCCACG GGAAGCTGAG CCTGACGGAG 
GTGGAGGGGC TGGCAGACCT GATCCATGCA GAAACCGAGG CGCAGCGGCG ACAGGCCCTG 
AGGCAGCTCG ATGGGGAGCT AGGTGACCTC TGTCGCGGCT GGGCCGAGAC CCTCACTAAA 
GCTCTGGCCC ATGTAGAGGC CTATATCGAT TTTAGTGAGG ATGACAACCT GGAGGAAGGC 
GTCCTGGATC AAGCTGATAG TGAGGTGCGG AAGCTGGAGG TGGCGCTGGG CGTACATCTT 
CGAGATGCCA GGCGTGGGCA GAGGCTCCGC TCAGGGGCGC ACGTGGTGGT TGCGGGACCT 
CCCAACGCCG GCAAAAGCAG CCTGGTGAAC CTGCTCAGCC GGAAGCCTGT GTCCATCGTG 
TCCCCGGAGC CGGGGACCAC CCGAGATGTT CTGGAGACCC CCGTGGACCT GGCCGGATTC 
CCAGCGCTGC TGAGCGACAC TGCGGGGTTG CGGGAAGGCG TGGGGCCTGT GGAGCAGGAG 
GGCGTGCGGC GTGCCCAAAA AAGGCTGGAG CAGGCTGACC TCATTCTGGC CGTGTTGGAT 
GCTTCTGACC TGGCCTCTCC GGCCAGCTGC AACTTCCTGG ACACCGTTGT CATCCCGGCA 
GGAGCCAGGA ACCCCAATGG GAGCCGCCAG CGCCTTCTGC TTGTGCTGAA TAAATCAGAC 
CTGCTGCCCG CTGGGCGCTC AGACCTCCAT CCTGACCTCC CCTCCCACCT GTTGCTGTCT 
TGCCTGACGG GTGTGGGGAC CCGTCCACAG GCCCACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804885.1
CDS	828..2309
Translation	MLLEEHHFTAPQRASPRLLSLSLASRSEDLQWMGGTFSTGPNDNRELLSTLPVTPRSGRNLLRGLADPRGRVGRRGADCESMWRGLWTLVSRAARGPHSPRLCTRQGSGYRAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALQSLTAPRDLPLARKACLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCAEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFAHGKLSLTEVEGLADLIHAETEAQRRQALRQLDGELGDLCRGWAETLTKALAHVEAYIDFSEDDNLEEGVLDQADSEVRKLEVALGVHLRDARRGQRLRSGAHVVVAGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPALLSDTAGLREGVGPVEQEGVRRAQKRLEQADLILAVLDASDLASPASCNFLDTVVIPAGARNPNGSRQRLLLVLNKSDLLPAGRSDLHPDLPSHLLLSCLTGVGTRPQAHF

Target ORF information:

RefSeq Version	XM_010806583.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GTP binding protein 3 (mitochondrial) (GTPBP3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010806583.2

ORF Insert Sequence:

ATGTTGCTGG AGGAACACCA TTTCACTGCA CCGCAGCGTG CGAGCCCTCG GCTTCTTTCC 
CTGTCATTAG CCAGTAGAAG CGAAGATCTT CAGTGGATGG GCGGTACGTT TTCTACGGGG 
CCCAATGATA ATCGAGAATT GTTGAGCACT TTACCAGTCA CGCCGCGGAG TGGGCGGAAC 
CTCCTGCGAG GACTTGCAGA CCCCCGCGGC AGAGTGGGCA GGCGGGGAGC TGATTGTGAA 
TCCATGTGGC GGGGGCTGTG GACCCTGGTA TCCCGGGCTG CACGTGGGCC TCACAGTCCC 
AGACTGTGCA CGCGCCAGGG CAGTGGTTAC CGGGCCCCCG GTTCCGGAGC CACTATCTTC 
GCTCTGAGCT CTGGCCAAGG CCGCTGCGGC ATCGCGGTGA TCCGAACCAG CGGCCCTGCC 
AGCGGCCACG CCCTCCAGAG CCTCACGGCG CCGCGGGACT TACCCCTGGC CCGCAAAGCC 
TGCCTGCGCC TGCTCAGCGA CCCCCGTTCT GGGGAGCCTT TGGACCGCGC GCTGGTGCTT 
TGGTTTCCAG GTCCCCAAAG TTTTACCGGT GAGGATTGCG CGGAGTTCCA CGTGCATGGA 
GGGCCAGCCG TGGTGAGTGG TGTCCTGCAG GCCCTGGGCA GTGTGCCAGG GCTGAGGCCA 
GCGGAAGCAG GCGAGTTCAC CAGGAGGGCG TTTGCCCACG GGAAGCTGAG CCTGACGGAG 
GTGGAGGGGC TGGCAGACCT GATCCATGCA GAAACCGAGG CGCAGCGGCG ACAGGCCCTG 
AGGCAGCTCG ATGGGGAGCT AGGTGACCTC TGTCGCGGCT GGGCCGAGAC CCTCACTAAA 
GCTCTGGCCC ATGTAGAGGC CTATATCGAT TTTAGTGAGG ATGACAACCT GGAGGAAGGC 
GTCCTGGATC AAGCTGATAG TGAGGTGCGG AAGCTGGAGG TGGCGCTGGG CGTACATCTT 
CGAGATGCCA GGCGTGGGCA GAGGCTCCGC TCAGGGGCGC ACGTGGTGGT TGCGGGACCT 
CCCAACGCCG GCAAAAGCAG CCTGGTGAAC CTGCTCAGCC GGAAGCCTGT GTCCATCGTG 
TCCCCGGAGC CGGGGACCAC CCGAGATGTT CTGGAGACCC CCGTGGACCT GGCCGGATTC 
CCAGCGCTGC TGAGCGACAC TGCGGGGTTG CGGGAAGGCG TGGGGCCTGT GGAGCAGGAG 
GGCGTGCGGC GTGCCCAAAA AAGGCTGGAG CAGGCTGACC TCATTCTGGC CGTGTTGGAT 
GCTTCTGACC TGGCCTCTCC GGCCAGCTGC AACTTCCTGG ACACCGTTGT CATCCCGGCA 
GGAGCCAGGA ACCCCAATGG GAGCCGCCAG CGCCTTCTGC TTGTGCTGAA TAAATCAGAC 
CTGCTGCCCG CTGGGCGCTC AGACCTCCAT CCTGACCTCC CCTCCCACCT GTTGCTGTCT 
TGCCTGACGG GTGTGGGGAC CCGTCCACAG GCCCACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTPBP3 ( XM_010806583.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804885.1 Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), transcript variant X3, mRNA.

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CloneID	OBa236192
Clone ID Related Accession (Same CDS sequence)	XM_010806583.3
Accession Version	XM_010806583.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tRNA modification GTPase GTPBP3, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806583.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGG AGGAACACCA TTTCACTGCA CCGCAGCGTG CGAGCCCTCG GCTTCTTTCC 
CTGTCATTAG CCAGTAGAAG CGAAGATCTT CAGTGGATGG GCGGTACGTT TTCTACGGGG 
CCCAATGATA ATCGAGAATT GTTGAGCACT TTACCAGTCA CGCCGCGGAG TGGGCGGAAC 
CTCCTGCGAG GACTTGCAGA CCCCCGCGGC AGAGTGGGCA GGCGGGGAGC TGATTGTGAA 
TCCATGTGGC GGGGGCTGTG GACCCTGGTA TCCCGGGCTG CACGTGGGCC TCACAGTCCC 
AGACTGTGCA CGCGCCAGGG CAGTGGTTAC CGGGCCCCCG GTTCCGGAGC CACTATCTTC 
GCTCTGAGCT CTGGCCAAGG CCGCTGCGGC ATCGCGGTGA TCCGAACCAG CGGCCCTGCC 
AGCGGCCACG CCCTCCAGAG CCTCACGGCG CCGCGGGACT TACCCCTGGC CCGCAAAGCC 
TGCCTGCGCC TGCTCAGCGA CCCCCGTTCT GGGGAGCCTT TGGACCGCGC GCTGGTGCTT 
TGGTTTCCAG GTCCCCAAAG TTTTACCGGT GAGGATTGCG CGGAGTTCCA CGTGCATGGA 
GGGCCAGCCG TGGTGAGTGG TGTCCTGCAG GCCCTGGGCA GTGTGCCAGG GCTGAGGCCA 
GCGGAAGCAG GCGAGTTCAC CAGGAGGGCG TTTGCCCACG GGAAGCTGAG CCTGACAGAG 
GTGGAGGGGC TGGCAGACCT GATCCATGCA GAAACCGAGG CGCAGCGGCG ACAGGCCCTG 
AGGCAGCTCG ATGGGGAGCT AGGTGACCTC TGTCGCGGCT GGGCCGAGAC CCTCACTAAA 
GCTCTGGCCC ATGTAGAGGC CTATATCGAT TTTAGTGAGG ATGACAACCT GGAGGAAGGC 
GTCCTGGATC AAGCTGATAG TGAGGTGCGG AAGCTGGAGG TGGCGCTGGG CGTACATCTT 
CGAGATGCCA GGCGTGGGCA GAGGCTCCGC TCAGGGGCGC ACGTGGTGGT TGCGGGACCT 
CCCAACGCCG GCAAAAGCAG CCTGGTGAAC CTGCTCAGCC GGAAGCCTGT GTCCATCGTG 
TCCCCGGAGC CGGGGACCAC CCGAGATGTT CTGGAGACCC CCGTGGACCT GGCCGGATTC 
CCAGCGCTGC TGAGCGACAC TGCGGGGTTG CGGGAAGGCG TGGGGCCTGT GGAGCAGGAG 
GGCGTGCGGC GTGCCCAAAA AAGGCTGGAG CAGGCTGACC TCATTCTGGC CGTGTTGGAT 
GCTTCTGACC TGGCCTCTCC GGCCAGCTGC AACTTCCTGG ACACCGTTGT CATCCCGGCA 
GGAGCCAGGA ACCCCAATGG GAGCCGCCAG CGCCTTCTGC TTGTGCTGAA TAAATCAGAC 
CTGCTGCCCG CTGGGCGCTC AGACCTCCAT CCTGACCTCC CCTCCCACCT GTTGCTGTCT 
TGCCTGACGG GTGTGGGGAC CCGTCCACAG GCCCACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804885.1
CDS	825..2306
Translation	MLLEEHHFTAPQRASPRLLSLSLASRSEDLQWMGGTFSTGPNDNRELLSTLPVTPRSGRNLLRGLADPRGRVGRRGADCESMWRGLWTLVSRAARGPHSPRLCTRQGSGYRAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALQSLTAPRDLPLARKACLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCAEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFAHGKLSLTEVEGLADLIHAETEAQRRQALRQLDGELGDLCRGWAETLTKALAHVEAYIDFSEDDNLEEGVLDQADSEVRKLEVALGVHLRDARRGQRLRSGAHVVVAGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPALLSDTAGLREGVGPVEQEGVRRAQKRLEQADLILAVLDASDLASPASCNFLDTVVIPAGARNPNGSRQRLLLVLNKSDLLPAGRSDLHPDLPSHLLLSCLTGVGTRPQAHF

Target ORF information:

RefSeq Version	XM_010806583.3
Organism	Bos taurus(cattle)
Definition	Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010806583.3

ORF Insert Sequence:

ATGTTGCTGG AGGAACACCA TTTCACTGCA CCGCAGCGTG CGAGCCCTCG GCTTCTTTCC 
CTGTCATTAG CCAGTAGAAG CGAAGATCTT CAGTGGATGG GCGGTACGTT TTCTACGGGG 
CCCAATGATA ATCGAGAATT GTTGAGCACT TTACCAGTCA CGCCGCGGAG TGGGCGGAAC 
CTCCTGCGAG GACTTGCAGA CCCCCGCGGC AGAGTGGGCA GGCGGGGAGC TGATTGTGAA 
TCCATGTGGC GGGGGCTGTG GACCCTGGTA TCCCGGGCTG CACGTGGGCC TCACAGTCCC 
AGACTGTGCA CGCGCCAGGG CAGTGGTTAC CGGGCCCCCG GTTCCGGAGC CACTATCTTC 
GCTCTGAGCT CTGGCCAAGG CCGCTGCGGC ATCGCGGTGA TCCGAACCAG CGGCCCTGCC 
AGCGGCCACG CCCTCCAGAG CCTCACGGCG CCGCGGGACT TACCCCTGGC CCGCAAAGCC 
TGCCTGCGCC TGCTCAGCGA CCCCCGTTCT GGGGAGCCTT TGGACCGCGC GCTGGTGCTT 
TGGTTTCCAG GTCCCCAAAG TTTTACCGGT GAGGATTGCG CGGAGTTCCA CGTGCATGGA 
GGGCCAGCCG TGGTGAGTGG TGTCCTGCAG GCCCTGGGCA GTGTGCCAGG GCTGAGGCCA 
GCGGAAGCAG GCGAGTTCAC CAGGAGGGCG TTTGCCCACG GGAAGCTGAG CCTGACAGAG 
GTGGAGGGGC TGGCAGACCT GATCCATGCA GAAACCGAGG CGCAGCGGCG ACAGGCCCTG 
AGGCAGCTCG ATGGGGAGCT AGGTGACCTC TGTCGCGGCT GGGCCGAGAC CCTCACTAAA 
GCTCTGGCCC ATGTAGAGGC CTATATCGAT TTTAGTGAGG ATGACAACCT GGAGGAAGGC 
GTCCTGGATC AAGCTGATAG TGAGGTGCGG AAGCTGGAGG TGGCGCTGGG CGTACATCTT 
CGAGATGCCA GGCGTGGGCA GAGGCTCCGC TCAGGGGCGC ACGTGGTGGT TGCGGGACCT 
CCCAACGCCG GCAAAAGCAG CCTGGTGAAC CTGCTCAGCC GGAAGCCTGT GTCCATCGTG 
TCCCCGGAGC CGGGGACCAC CCGAGATGTT CTGGAGACCC CCGTGGACCT GGCCGGATTC 
CCAGCGCTGC TGAGCGACAC TGCGGGGTTG CGGGAAGGCG TGGGGCCTGT GGAGCAGGAG 
GGCGTGCGGC GTGCCCAAAA AAGGCTGGAG CAGGCTGACC TCATTCTGGC CGTGTTGGAT 
GCTTCTGACC TGGCCTCTCC GGCCAGCTGC AACTTCCTGG ACACCGTTGT CATCCCGGCA 
GGAGCCAGGA ACCCCAATGG GAGCCGCCAG CGCCTTCTGC TTGTGCTGAA TAAATCAGAC 
CTGCTGCCCG CTGGGCGCTC AGACCTCCAT CCTGACCTCC CCTCCCACCT GTTGCTGTCT 
TGCCTGACGG GTGTGGGGAC CCGTCCACAG GCCCACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTPBP3 ( NM_001206218.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193147.1 Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), mRNA.

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CloneID	OBa239187
Clone ID Related Accession (Same CDS sequence)	NM_001206218.2
Accession Version	NM_001206218.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-13
Organism	Bos taurus(cattle)
Product	tRNA modification GTPase GTPBP3, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000007.1. On May 30, 2018 this sequence version replaced NM_001206218.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTGGCGGG GGCTGTGGAC CCTGGTATCC CGGGCTGCAC GTGGGCCTCA CAGACTGTGC 
ACGCGCCAGG GCAGTGGTTA CCGGGCCCCC GGTTCCGGAG CCACTATCTT CGCTCTGAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCGGTG ATCCGAACCA GCGGCCCTGC CAGCGGCCAC 
GCCCTCCAGA GCCTCACGGC GCCGCGGGAC TTACCCCTGG CCCGCAAAGC CTGCCTGCGC 
CTGCTCAGCG ACCCCCGTTC TGGGGAGCCT TTGGACCGCG CGCTGGTGCT TTGGTTTCCA 
GGTCCCCAAA GTTTTACCGG TGAGGATTGC GCGGAGTTCC ACGTGCATGG AGGGCCAGCC 
GTGGTGAGTG GTGTCCTGCA GGCCCTGGGC AGTGTGCCAG GGCTGAGGCC AGCGGAAGCA 
GGCGAGTTCA CCAGGAGGGC GTTTGCCCAC GGGAAGCTGA GCCTGACAGA GGTGGAGGGG 
CTGGCAGACC TGATCCATGC AGAAACCGAG GCGCAGCGGC GACAGGCCCT GAGGCAGCTC 
GATGGGGAGC TAGGTGACCT CTGTCGCGGC TGGGCCGAGA CCCTCACTAA AGCTCTGGCC 
CATGTAGAGG CCTATATCGA TTTTAGTGAG GATGACAACC TGGAGGAAGG CGTCCTGGAT 
CAAGGTGGGT CCCCCTGGGG GTGGGAGGCG GAGACATCTG GCATCCAGCC CCCCCAGGTC 
CTCTTACTCT CTCTCTCCTT TCCTCCATCC ACAGCTGATA GTGAGGTGCG GAAGCTGGAG 
GTGGCGCTGG GCGTACATCT TCGAGATGCC AGGCGTGGGC AGAGGCTCCG CTCAGGGGCG 
CACGTGGTGG TTGCGGGACC TCCCAACGCC GGCAAAAGCA GCCTGGTGAA CCTGCTCAGC 
CGGAAGCCTG TGTCCATCGT GTCCCCGGAG CCGGGGACCA CCCGAGATGT TCTGGAGACC 
CCCGTGGACC TGGCCGGATT CCCAGCGCTG CTGAGCGACA CTGCGGGGTT GCGGGAAGGC 
GTGGGGCCTG TGGAGCAGGA GGGCGTGCGG CGTGCCCAAA AAAGGCTGGA GCAGGCTGAC 
CTCATTCTGG CCGTGTTGGA TGCTTCTGAC CTGGCCTCTC CGGCCAGCTG CAACTTCCTG 
GACACCGTTG TCATCCCGGC AGGAGCCAGG AACCCCAATG GGAGCCGCCA GCGCCTTCTG 
CTTGTGCTGA ATAAATCAGA CCTGCTGCCC GCTGGGCGCT CAGACCTCCA TCCTGACCTC 
CCCTCCCACC TGTTGCTGTC TTGCCTGACG GGTGAGGGGC TGGACGGCCT CCTAGAAGCA 
CTGAAGAAGG AGCTGGCTGC ACTGTGTGGG GACCCGTCCA CAGGCCCACT TCTGACACGG 
TCGAGGCACC AACACCACCT CCGGGGCTGC CTGGATGCCC TTGGTCAGTA CAAGCAGGCA 
AAAGACTTAG CCCTGGCAGC CGAGGCACTT CGGGTAGCCC GAGGGCATCT GAGTCATCTC 
ACTGGTGGAG GGGGCACTGA GGAAATTCTG GATATCATCT TCCGGGACTT CTGCGTGGGC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193147.1
CDS	1..1566
Translation	MWRGLWTLVSRAARGPHRLCTRQGSGYRAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALQSLTAPRDLPLARKACLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCAEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFAHGKLSLTEVEGLADLIHAETEAQRRQALRQLDGELGDLCRGWAETLTKALAHVEAYIDFSEDDNLEEGVLDQGGSPWGWEAETSGIQPPQVLLLSLSFPPSTADSEVRKLEVALGVHLRDARRGQRLRSGAHVVVAGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPALLSDTAGLREGVGPVEQEGVRRAQKRLEQADLILAVLDASDLASPASCNFLDTVVIPAGARNPNGSRQRLLLVLNKSDLLPAGRSDLHPDLPSHLLLSCLTGEGLDGLLEALKKELAALCGDPSTGPLLTRSRHQHHLRGCLDALGQYKQAKDLALAAEALRVARGHLSHLTGGGGTEEILDIIFRDFCVGK

Target ORF information:

RefSeq Version	NM_001206218.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206218.2

ORF Insert Sequence:

ATGTGGCGGG GGCTGTGGAC CCTGGTATCC CGGGCTGCAC GTGGGCCTCA CAGACTGTGC 
ACGCGCCAGG GCAGTGGTTA CCGGGCCCCC GGTTCCGGAG CCACTATCTT CGCTCTGAGC 
TCTGGCCAAG GCCGCTGCGG CATCGCGGTG ATCCGAACCA GCGGCCCTGC CAGCGGCCAC 
GCCCTCCAGA GCCTCACGGC GCCGCGGGAC TTACCCCTGG CCCGCAAAGC CTGCCTGCGC 
CTGCTCAGCG ACCCCCGTTC TGGGGAGCCT TTGGACCGCG CGCTGGTGCT TTGGTTTCCA 
GGTCCCCAAA GTTTTACCGG TGAGGATTGC GCGGAGTTCC ACGTGCATGG AGGGCCAGCC 
GTGGTGAGTG GTGTCCTGCA GGCCCTGGGC AGTGTGCCAG GGCTGAGGCC AGCGGAAGCA 
GGCGAGTTCA CCAGGAGGGC GTTTGCCCAC GGGAAGCTGA GCCTGACAGA GGTGGAGGGG 
CTGGCAGACC TGATCCATGC AGAAACCGAG GCGCAGCGGC GACAGGCCCT GAGGCAGCTC 
GATGGGGAGC TAGGTGACCT CTGTCGCGGC TGGGCCGAGA CCCTCACTAA AGCTCTGGCC 
CATGTAGAGG CCTATATCGA TTTTAGTGAG GATGACAACC TGGAGGAAGG CGTCCTGGAT 
CAAGGTGGGT CCCCCTGGGG GTGGGAGGCG GAGACATCTG GCATCCAGCC CCCCCAGGTC 
CTCTTACTCT CTCTCTCCTT TCCTCCATCC ACAGCTGATA GTGAGGTGCG GAAGCTGGAG 
GTGGCGCTGG GCGTACATCT TCGAGATGCC AGGCGTGGGC AGAGGCTCCG CTCAGGGGCG 
CACGTGGTGG TTGCGGGACC TCCCAACGCC GGCAAAAGCA GCCTGGTGAA CCTGCTCAGC 
CGGAAGCCTG TGTCCATCGT GTCCCCGGAG CCGGGGACCA CCCGAGATGT TCTGGAGACC 
CCCGTGGACC TGGCCGGATT CCCAGCGCTG CTGAGCGACA CTGCGGGGTT GCGGGAAGGC 
GTGGGGCCTG TGGAGCAGGA GGGCGTGCGG CGTGCCCAAA AAAGGCTGGA GCAGGCTGAC 
CTCATTCTGG CCGTGTTGGA TGCTTCTGAC CTGGCCTCTC CGGCCAGCTG CAACTTCCTG 
GACACCGTTG TCATCCCGGC AGGAGCCAGG AACCCCAATG GGAGCCGCCA GCGCCTTCTG 
CTTGTGCTGA ATAAATCAGA CCTGCTGCCC GCTGGGCGCT CAGACCTCCA TCCTGACCTC 
CCCTCCCACC TGTTGCTGTC TTGCCTGACG GGTGAGGGGC TGGACGGCCT CCTAGAAGCA 
CTGAAGAAGG AGCTGGCTGC ACTGTGTGGG GACCCGTCCA CAGGCCCACT TCTGACACGG 
TCGAGGCACC AACACCACCT CCGGGGCTGC CTGGATGCCC TTGGTCAGTA CAAGCAGGCA 
AAAGACTTAG CCCTGGCAGC CGAGGCACTT CGGGTAGCCC GAGGGCATCT GAGTCATCTC 
ACTGGTGGAG GGGGCACTGA GGAAATTCTG GATATCATCT TCCGGGACTT CTGCGTGGGC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTPBP3 ( XM_010806582.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804884.1 Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), transcript variant X1, mRNA.

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CloneID	OBa236190
Clone ID Related Accession (Same CDS sequence)	XM_010806582.3
Accession Version	XM_010806582.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tRNA modification GTPase GTPBP3, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806582.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGG AGGAACACCA TTTCACTGCA CCGCAGCGTG CGAGCCCTCG GCTTCTTTCC 
CTGTCATTAG CCAGTAGAAG CGAAGATCTT CAGTGGATGG GCGGTACGTT TTCTACGGGG 
CCCAATGATA ATCGAGAATT GTTGAGCACT TTACCAGTCA CGCCGCGGAG TGGGCGGAAC 
CTCCTGCGAG GACTTGCAGA CCCCCGCGGC AGAGTGGGCA GGCGGGGAGC TGATTGTGAA 
TCCATGTGGC GGGGGCTGTG GACCCTGGTA TCCCGGGCTG CACGTGGGCC TCACAGTCCC 
AGACTGTGCA CGCGCCAGGG CAGTGGTTAC CGGGCCCCCG GTTCCGGAGC CACTATCTTC 
GCTCTGAGCT CTGGCCAAGG CCGCTGCGGC ATCGCGGTGA TCCGAACCAG CGGCCCTGCC 
AGCGGCCACG CCCTCCAGAG CCTCACGGCG CCGCGGGACT TACCCCTGGC CCGCAAAGCC 
TGCCTGCGCC TGCTCAGCGA CCCCCGTTCT GGGGAGCCTT TGGACCGCGC GCTGGTGCTT 
TGGTTTCCAG GTCCCCAAAG TTTTACCGGT GAGGATTGCG CGGAGTTCCA CGTGCATGGA 
GGGCCAGCCG TGGTGAGTGG TGTCCTGCAG GCCCTGGGCA GTGTGCCAGG GCTGAGGCCA 
GCGGAAGCAG GCGAGTTCAC CAGGAGGGCG TTTGCCCACG GGAAGCTGAG CCTGACAGAG 
GTGGAGGGGC TGGCAGACCT GATCCATGCA GAAACCGAGG CGCAGCGGCG ACAGGCCCTG 
AGGCAGCTCG ATGGGGAGCT AGGTGACCTC TGTCGCGGCT GGGCCGAGAC CCTCACTAAA 
GCTCTGGCCC ATGTAGAGGC CTATATCGAT TTTAGTGAGG ATGACAACCT GGAGGAAGGC 
GTCCTGGATC AAGGTGGGTC CCCCTGGGGG TGGGAGGCGG AGACATCTGG CATCCAGCCC 
CCCCAGGTCC TCTTACTCTC TCTCTCCTTT CCTCCATCCA CAGCTGATAG TGAGGTGCGG 
AAGCTGGAGG TGGCGCTGGG CGTACATCTT CGAGATGCCA GGCGTGGGCA GAGGCTCCGC 
TCAGGGGCGC ACGTGGTGGT TGCGGGACCT CCCAACGCCG GCAAAAGCAG CCTGGTGAAC 
CTGCTCAGCC GGAAGCCTGT GTCCATCGTG TCCCCGGAGC CGGGGACCAC CCGAGATGTT 
CTGGAGACCC CCGTGGACCT GGCCGGATTC CCAGCGCTGC TGAGCGACAC TGCGGGGTTG 
CGGGAAGGCG TGGGGCCTGT GGAGCAGGAG GGCGTGCGGC GTGCCCAAAA AAGGCTGGAG 
CAGGCTGACC TCATTCTGGC CGTGTTGGAT GCTTCTGACC TGGCCTCTCC GGCCAGCTGC 
AACTTCCTGG ACACCGTTGT CATCCCGGCA GGAGCCAGGA ACCCCAATGG GAGCCGCCAG 
CGCCTTCTGC TTGTGCTGAA TAAATCAGAC CTGCTGCCCG CTGGGCGCTC AGACCTCCAT 
CCTGACCTCC CCTCCCACCT GTTGCTGTCT TGCCTGACGG GTGAGGGGCT GGACGGCCTC 
CTAGAAGCAC TGAAGAAGGA GCTGGCTGCA CTGTGTGGGG ACCCGTCCAC AGGCCCACTT 
CTGACACGGT CGAGGCACCA ACACCACCTC CGGGGCTGCC TGGATGCCCT TGGTCAGTAC 
AAGCAGGCAA AAGACTTAGC CCTGGCAGCC GAGGCACTTC GGGTAGCCCG AGGGCATCTG 
AGTCATCTCA CTGGTGGAGG GGGCACTGAG GAAATTCTGG ATATCATCTT CCGGGACTTC 
TGCGTGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804884.1
CDS	825..2639
Translation	MLLEEHHFTAPQRASPRLLSLSLASRSEDLQWMGGTFSTGPNDNRELLSTLPVTPRSGRNLLRGLADPRGRVGRRGADCESMWRGLWTLVSRAARGPHSPRLCTRQGSGYRAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALQSLTAPRDLPLARKACLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCAEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFAHGKLSLTEVEGLADLIHAETEAQRRQALRQLDGELGDLCRGWAETLTKALAHVEAYIDFSEDDNLEEGVLDQGGSPWGWEAETSGIQPPQVLLLSLSFPPSTADSEVRKLEVALGVHLRDARRGQRLRSGAHVVVAGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPALLSDTAGLREGVGPVEQEGVRRAQKRLEQADLILAVLDASDLASPASCNFLDTVVIPAGARNPNGSRQRLLLVLNKSDLLPAGRSDLHPDLPSHLLLSCLTGEGLDGLLEALKKELAALCGDPSTGPLLTRSRHQHHLRGCLDALGQYKQAKDLALAAEALRVARGHLSHLTGGGGTEEILDIIFRDFCVGK

Target ORF information:

RefSeq Version	XM_010806582.3
Organism	Bos taurus(cattle)
Definition	Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010806582.3

ORF Insert Sequence:

ATGTTGCTGG AGGAACACCA TTTCACTGCA CCGCAGCGTG CGAGCCCTCG GCTTCTTTCC 
CTGTCATTAG CCAGTAGAAG CGAAGATCTT CAGTGGATGG GCGGTACGTT TTCTACGGGG 
CCCAATGATA ATCGAGAATT GTTGAGCACT TTACCAGTCA CGCCGCGGAG TGGGCGGAAC 
CTCCTGCGAG GACTTGCAGA CCCCCGCGGC AGAGTGGGCA GGCGGGGAGC TGATTGTGAA 
TCCATGTGGC GGGGGCTGTG GACCCTGGTA TCCCGGGCTG CACGTGGGCC TCACAGTCCC 
AGACTGTGCA CGCGCCAGGG CAGTGGTTAC CGGGCCCCCG GTTCCGGAGC CACTATCTTC 
GCTCTGAGCT CTGGCCAAGG CCGCTGCGGC ATCGCGGTGA TCCGAACCAG CGGCCCTGCC 
AGCGGCCACG CCCTCCAGAG CCTCACGGCG CCGCGGGACT TACCCCTGGC CCGCAAAGCC 
TGCCTGCGCC TGCTCAGCGA CCCCCGTTCT GGGGAGCCTT TGGACCGCGC GCTGGTGCTT 
TGGTTTCCAG GTCCCCAAAG TTTTACCGGT GAGGATTGCG CGGAGTTCCA CGTGCATGGA 
GGGCCAGCCG TGGTGAGTGG TGTCCTGCAG GCCCTGGGCA GTGTGCCAGG GCTGAGGCCA 
GCGGAAGCAG GCGAGTTCAC CAGGAGGGCG TTTGCCCACG GGAAGCTGAG CCTGACAGAG 
GTGGAGGGGC TGGCAGACCT GATCCATGCA GAAACCGAGG CGCAGCGGCG ACAGGCCCTG 
AGGCAGCTCG ATGGGGAGCT AGGTGACCTC TGTCGCGGCT GGGCCGAGAC CCTCACTAAA 
GCTCTGGCCC ATGTAGAGGC CTATATCGAT TTTAGTGAGG ATGACAACCT GGAGGAAGGC 
GTCCTGGATC AAGGTGGGTC CCCCTGGGGG TGGGAGGCGG AGACATCTGG CATCCAGCCC 
CCCCAGGTCC TCTTACTCTC TCTCTCCTTT CCTCCATCCA CAGCTGATAG TGAGGTGCGG 
AAGCTGGAGG TGGCGCTGGG CGTACATCTT CGAGATGCCA GGCGTGGGCA GAGGCTCCGC 
TCAGGGGCGC ACGTGGTGGT TGCGGGACCT CCCAACGCCG GCAAAAGCAG CCTGGTGAAC 
CTGCTCAGCC GGAAGCCTGT GTCCATCGTG TCCCCGGAGC CGGGGACCAC CCGAGATGTT 
CTGGAGACCC CCGTGGACCT GGCCGGATTC CCAGCGCTGC TGAGCGACAC TGCGGGGTTG 
CGGGAAGGCG TGGGGCCTGT GGAGCAGGAG GGCGTGCGGC GTGCCCAAAA AAGGCTGGAG 
CAGGCTGACC TCATTCTGGC CGTGTTGGAT GCTTCTGACC TGGCCTCTCC GGCCAGCTGC 
AACTTCCTGG ACACCGTTGT CATCCCGGCA GGAGCCAGGA ACCCCAATGG GAGCCGCCAG 
CGCCTTCTGC TTGTGCTGAA TAAATCAGAC CTGCTGCCCG CTGGGCGCTC AGACCTCCAT 
CCTGACCTCC CCTCCCACCT GTTGCTGTCT TGCCTGACGG GTGAGGGGCT GGACGGCCTC 
CTAGAAGCAC TGAAGAAGGA GCTGGCTGCA CTGTGTGGGG ACCCGTCCAC AGGCCCACTT 
CTGACACGGT CGAGGCACCA ACACCACCTC CGGGGCTGCC TGGATGCCCT TGGTCAGTAC 
AAGCAGGCAA AAGACTTAGC CCTGGCAGCC GAGGCACTTC GGGTAGCCCG AGGGCATCTG 
AGTCATCTCA CTGGTGGAGG GGGCACTGAG GAAATTCTGG ATATCATCTT CCGGGACTTC 
TGCGTGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GTPBP3 ( XM_005208516.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208573.1 Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), transcript variant X2, mRNA.

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CloneID	OBa236191
Clone ID Related Accession (Same CDS sequence)	XM_005208516.4
Accession Version	XM_005208516.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tRNA modification GTPase GTPBP3, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208516.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGG AGGAACACCA TTTCACTGCA CCGCAGCGTG CGAGCCCTCG GCTTCTTTCC 
CTGTCATTAG CCAGTAGAAG CGAAGATCTT CAGTGGATGG GCGGTACGTT TTCTACGGGG 
CCCAATGATA ATCGAGAATT GTTGAGCACT TTACCAGTCA CGCCGCGGAG TGGGCGGAAC 
CTCCTGCGAG GACTTGCAGA CCCCCGCGGC AGAGTGGGCA GGCGGGGAGC TGATTGTGAA 
TCCATGTGGC GGGGGCTGTG GACCCTGGTA TCCCGGGCTG CACGTGGGCC TCACAGTCCC 
AGACTGTGCA CGCGCCAGGG CAGTGGTTAC CGGGCCCCCG GTTCCGGAGC CACTATCTTC 
GCTCTGAGCT CTGGCCAAGG CCGCTGCGGC ATCGCGGTGA TCCGAACCAG CGGCCCTGCC 
AGCGGCCACG CCCTCCAGAG CCTCACGGCG CCGCGGGACT TACCCCTGGC CCGCAAAGCC 
TGCCTGCGCC TGCTCAGCGA CCCCCGTTCT GGGGAGCCTT TGGACCGCGC GCTGGTGCTT 
TGGTTTCCAG GTCCCCAAAG TTTTACCGGT GAGGATTGCG CGGAGTTCCA CGTGCATGGA 
GGGCCAGCCG TGGTGAGTGG TGTCCTGCAG GCCCTGGGCA GTGTGCCAGG GCTGAGGCCA 
GCGGAAGCAG GCGAGTTCAC CAGGAGGGCG TTTGCCCACG GGAAGCTGAG CCTGACAGAG 
GTGGAGGGGC TGGCAGACCT GATCCATGCA GAAACCGAGG CGCAGCGGCG ACAGGCCCTG 
AGGCAGCTCG ATGGGGAGCT AGGTGACCTC TGTCGCGGCT GGGCCGAGAC CCTCACTAAA 
GCTCTGGCCC ATGTAGAGGC CTATATCGAT TTTAGTGAGG ATGACAACCT GGAGGAAGGC 
GTCCTGGATC AAGCTGATAG TGAGGTGCGG AAGCTGGAGG TGGCGCTGGG CGTACATCTT 
CGAGATGCCA GGCGTGGGCA GAGGCTCCGC TCAGGGGCGC ACGTGGTGGT TGCGGGACCT 
CCCAACGCCG GCAAAAGCAG CCTGGTGAAC CTGCTCAGCC GGAAGCCTGT GTCCATCGTG 
TCCCCGGAGC CGGGGACCAC CCGAGATGTT CTGGAGACCC CCGTGGACCT GGCCGGATTC 
CCAGCGCTGC TGAGCGACAC TGCGGGGTTG CGGGAAGGCG TGGGGCCTGT GGAGCAGGAG 
GGCGTGCGGC GTGCCCAAAA AAGGCTGGAG CAGGCTGACC TCATTCTGGC CGTGTTGGAT 
GCTTCTGACC TGGCCTCTCC GGCCAGCTGC AACTTCCTGG ACACCGTTGT CATCCCGGCA 
GGAGCCAGGA ACCCCAATGG GAGCCGCCAG CGCCTTCTGC TTGTGCTGAA TAAATCAGAC 
CTGCTGCCCG CTGGGCGCTC AGACCTCCAT CCTGACCTCC CCTCCCACCT GTTGCTGTCT 
TGCCTGACGG GTGAGGGGCT GGACGGCCTC CTAGAAGCAC TGAAGAAGGA GCTGGCTGCA 
CTGTGTGGGG ACCCGTCCAC AGGCCCACTT CTGACACGGT CGAGGCACCA ACACCACCTC 
CGGGGCTGCC TGGATGCCCT TGGTCAGTAC AAGCAGGCAA AAGACTTAGC CCTGGCAGCC 
GAGGCACTTC GGGTAGCCCG AGGGCATCTG AGTCATCTCA CTGGTGGAGG GGGCACTGAG 
GAAATTCTGG ATATCATCTT CCGGGACTTC TGCGTGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208573.1
CDS	825..2549
Translation	MLLEEHHFTAPQRASPRLLSLSLASRSEDLQWMGGTFSTGPNDNRELLSTLPVTPRSGRNLLRGLADPRGRVGRRGADCESMWRGLWTLVSRAARGPHSPRLCTRQGSGYRAPGSGATIFALSSGQGRCGIAVIRTSGPASGHALQSLTAPRDLPLARKACLRLLSDPRSGEPLDRALVLWFPGPQSFTGEDCAEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFAHGKLSLTEVEGLADLIHAETEAQRRQALRQLDGELGDLCRGWAETLTKALAHVEAYIDFSEDDNLEEGVLDQADSEVRKLEVALGVHLRDARRGQRLRSGAHVVVAGPPNAGKSSLVNLLSRKPVSIVSPEPGTTRDVLETPVDLAGFPALLSDTAGLREGVGPVEQEGVRRAQKRLEQADLILAVLDASDLASPASCNFLDTVVIPAGARNPNGSRQRLLLVLNKSDLLPAGRSDLHPDLPSHLLLSCLTGEGLDGLLEALKKELAALCGDPSTGPLLTRSRHQHHLRGCLDALGQYKQAKDLALAAEALRVARGHLSHLTGGGGTEEILDIIFRDFCVGK

Target ORF information:

RefSeq Version	XM_005208516.4
Organism	Bos taurus(cattle)
Definition	Bos taurus GTP binding protein 3, mitochondrial (GTPBP3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208516.4

ORF Insert Sequence:

ATGTTGCTGG AGGAACACCA TTTCACTGCA CCGCAGCGTG CGAGCCCTCG GCTTCTTTCC 
CTGTCATTAG CCAGTAGAAG CGAAGATCTT CAGTGGATGG GCGGTACGTT TTCTACGGGG 
CCCAATGATA ATCGAGAATT GTTGAGCACT TTACCAGTCA CGCCGCGGAG TGGGCGGAAC 
CTCCTGCGAG GACTTGCAGA CCCCCGCGGC AGAGTGGGCA GGCGGGGAGC TGATTGTGAA 
TCCATGTGGC GGGGGCTGTG GACCCTGGTA TCCCGGGCTG CACGTGGGCC TCACAGTCCC 
AGACTGTGCA CGCGCCAGGG CAGTGGTTAC CGGGCCCCCG GTTCCGGAGC CACTATCTTC 
GCTCTGAGCT CTGGCCAAGG CCGCTGCGGC ATCGCGGTGA TCCGAACCAG CGGCCCTGCC 
AGCGGCCACG CCCTCCAGAG CCTCACGGCG CCGCGGGACT TACCCCTGGC CCGCAAAGCC 
TGCCTGCGCC TGCTCAGCGA CCCCCGTTCT GGGGAGCCTT TGGACCGCGC GCTGGTGCTT 
TGGTTTCCAG GTCCCCAAAG TTTTACCGGT GAGGATTGCG CGGAGTTCCA CGTGCATGGA 
GGGCCAGCCG TGGTGAGTGG TGTCCTGCAG GCCCTGGGCA GTGTGCCAGG GCTGAGGCCA 
GCGGAAGCAG GCGAGTTCAC CAGGAGGGCG TTTGCCCACG GGAAGCTGAG CCTGACAGAG 
GTGGAGGGGC TGGCAGACCT GATCCATGCA GAAACCGAGG CGCAGCGGCG ACAGGCCCTG 
AGGCAGCTCG ATGGGGAGCT AGGTGACCTC TGTCGCGGCT GGGCCGAGAC CCTCACTAAA 
GCTCTGGCCC ATGTAGAGGC CTATATCGAT TTTAGTGAGG ATGACAACCT GGAGGAAGGC 
GTCCTGGATC AAGCTGATAG TGAGGTGCGG AAGCTGGAGG TGGCGCTGGG CGTACATCTT 
CGAGATGCCA GGCGTGGGCA GAGGCTCCGC TCAGGGGCGC ACGTGGTGGT TGCGGGACCT 
CCCAACGCCG GCAAAAGCAG CCTGGTGAAC CTGCTCAGCC GGAAGCCTGT GTCCATCGTG 
TCCCCGGAGC CGGGGACCAC CCGAGATGTT CTGGAGACCC CCGTGGACCT GGCCGGATTC 
CCAGCGCTGC TGAGCGACAC TGCGGGGTTG CGGGAAGGCG TGGGGCCTGT GGAGCAGGAG 
GGCGTGCGGC GTGCCCAAAA AAGGCTGGAG CAGGCTGACC TCATTCTGGC CGTGTTGGAT 
GCTTCTGACC TGGCCTCTCC GGCCAGCTGC AACTTCCTGG ACACCGTTGT CATCCCGGCA 
GGAGCCAGGA ACCCCAATGG GAGCCGCCAG CGCCTTCTGC TTGTGCTGAA TAAATCAGAC 
CTGCTGCCCG CTGGGCGCTC AGACCTCCAT CCTGACCTCC CCTCCCACCT GTTGCTGTCT 
TGCCTGACGG GTGAGGGGCT GGACGGCCTC CTAGAAGCAC TGAAGAAGGA GCTGGCTGCA 
CTGTGTGGGG ACCCGTCCAC AGGCCCACTT CTGACACGGT CGAGGCACCA ACACCACCTC 
CGGGGCTGCC TGGATGCCCT TGGTCAGTAC AAGCAGGCAA AAGACTTAGC CCTGGCAGCC 
GAGGCACTTC GGGTAGCCCG AGGGCATCTG AGTCATCTCA CTGGTGGAGG GGGCACTGAG 
GAAATTCTGG ATATCATCTT CCGGGACTTC TGCGTGGGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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GTPBP3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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