HELZ cDNA ORF clone, Bos taurus(cattle)

The following HELZ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HELZ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227074 XM_024981133.1
Latest version!
Bos taurus helicase with zinc finger (HELZ), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227074 XM_024981134.1
Latest version!
Bos taurus helicase with zinc finger (HELZ), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227074 XM_024981131.1
Latest version!
Bos taurus helicase with zinc finger (HELZ), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227074 XM_024981135.1
Latest version!
Bos taurus helicase with zinc finger (HELZ), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227074 XM_024981132.1
Latest version!
Bos taurus helicase with zinc finger (HELZ), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227075 XM_024981136.1
Latest version!
Bos taurus helicase with zinc finger (HELZ), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227075 XM_024981137.1
Latest version!
Bos taurus helicase with zinc finger (HELZ), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227075 XM_024981139.1
Latest version!
Bos taurus helicase with zinc finger (HELZ), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227076 XM_024981140.1
Latest version!
Bos taurus helicase with zinc finger (HELZ), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227077 XM_024981141.1
Latest version!
Bos taurus helicase with zinc finger (HELZ), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa94083 XM_015459037.1
Latest version!
Bos taurus helicase with zinc finger (HELZ), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa95076 XM_015468293.1
Latest version!
Bos taurus helicase with zinc finger (HELZ), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa227074
    Clone ID Related Accession (Same CDS sequence) XM_024981135.1 , XM_024981134.1 , XM_024981131.1 , XM_024981133.1 , XM_024981132.1
    Accession Version XM_024981135.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable helicase with zinc finger domain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGCAGGA TTCTCATTTG CACCCATTCT
    AATAGTGCTG CTGATCTCTA CATAAAGGAT TATTTACATC CATATGTAGA AGCAGGCAAT
    CCCCAGGCAA GACCTCTCAG GGTATATTTC AGAAACCGCT GGGTGAAGAC TGTCCACCCA
    GTTGTGCATC AGTACTGTTT GATCTCAAGC GCACATTCCA CCTTTCAGAT GCCACAGAAA
    GAAGATATTC TGAAACACCG AGTGGTGGTG GTTACATTGA ACACTTCCCA GTACCTCTGT
    CAGCTGGACC TCGAACCTGG GTTTTTTACT CACATCTTGT TAGACGAGGC TGCCCAGGCC
    ATGGAGTGTG AAACCATCAT GCCTCTGGCA CTAGCAACTA GACACACGCG GGTTGTCCTG
    GCTGGCGACC ACATGCAGCT CAGTCCTTTT GTTTACAGCG AGTTTGCCAG GGAGAGGAAC
    CTTCACGTGT CGTTACTTGA TCGGCTCTAT GAGCACTACC CTGCCGAGTT TCCCTGTAGG
    ATCCTCCTGT GCGAGAACTA CCGTTCCCAC GAAGCTATCA TCAATTACAC CTCTGAGCTC
    TTCTATGAGG GCAAGCTGAT GGCCAGTGGA AAGCAACCAG CACACAAGGA GTTCTATCCA
    CTAACCTTCT TTACAGCACG AGGGGAAGAT GTACAGGAGA AAAATAGCAC AGCTTTTTAT
    AATAATGCAG AGGTATTTGA AGTGGTGGAG CGAGTGGAAG AGCTGAGAAG GAAGTGGCCA
    GTAGCGTGGG GCAAGTTGGA TGATGGAAGT ATTGGTGTGG TGACTCCATA TGCAGACCAG
    GTGTTCAGGA TACGCGCCGA ACTTCGAAAA AAGAGGTTAT CTGACGTTAA CGTAGAAAGG
    GTGCTCAATG TTCAAGGAAA GCAATTCAGA GTTTTGTTTC TTAGCACAGT ACGTACAAGG
    CATACTTGTA AACATAAACA GACACCAATT AAAAAGAAGG AGCAGCTGCT AGAAGACTCC
    ACAGAGGACT TGGATTATGG CTTTTTGTCT AACTACAAGC TCCTCAACAC CGCCATCACA
    AGAGCACAGT CCCTGGTTGC CGTGGTGGGG GACCCCATCG CTCTGTGCTC CATTGGGAGA
    TGCAGGAAAT TTTGGGAGCG GTTTATTGCC CTGTGTCATG AAAACAATAG CCTGCATGGA
    ATCACTTTTG AACAGATCAA AGCGCAGCTA GAGGCTTTAG AACTAAAGAA GACATACGTG
    TTGAATCCAC TGGCACCTGA ATTTATACCC CGGGCTCTAA GAGCACAGCA CGCGGGAAAC
    ACCAACAAAC CGCAGCAATC ACCCCCCAAG GGAAAAAGCC TCCATCATAC CCAGAATGAT
    CATTTCCAGA ATGATGGAAT TGTTCAGCCC AATCCCTCTG TGCTTATTGG CAATCCTATC
    AGAGCATATA CTCCTCCACC TCCTCTTGGA CCTCACCCAA ATTTGGGGAA ATCTCCAAGT
    CCTGTTCAAA GAATCGATCC TCACACTGGG ACGAGTATTC TTTATGTACC TGCTGTCTAT
    GGAGGGAATG TCGTTATGTC GGTGCCTGTA CCTGTGCCAT GGACAGGATA CCAGGGCAGG
    TTTGCGGTTG ATCCTCGAAT CATTACACAC CAGGCAGCAA TGGCCTACAA CATGAACCTC
    TTACAGACAC ATGGACGTGG GTCTCCTATT CCTTATGGCC TTGGGCACCA CCCACCTGTC
    AGCATAGGGC AGCCACAGAA TCAGCATCAG GAGAAGGATC AACATGAGCA AAGTCGCAAC
    GGTAAAAGTG ATACAAATAA TCCTGGACCT GAAATTAATA AGATTCGAAC ACCAGAGAAA
    AAACCTACAG AAGCAAAACA GATTGATTTG GAATCAAACC CACAGAACAG AAGTCCTGAA
    TCACGTTCTG GCATTGTTTA TCCCCATCCC AAATTTCATC GCAAAGATAA TCTCACCCCA
    AGACACATAA ACCTTCCTCT CCCTGCCCCC CATGCCCAGT ATGCCATCCC CGGTCGTCAT
    TTTCATCCCC TCCCCCAGCT CCCGAGACCA CCCTTCCCAA TTCCGCAGCA GCACGCCTTG
    CTGAATCAGC AGCAGAGTAA CTTGCCTGAA CAGCCGAGCC CCGTGCCAGC CCAGCCCAGT
    CAGGTGGCCC CTCAGCTGGG CCCGCCGCCG CCTCAGCTCT CCCCTGCCTA CCCGGCTGGC
    CCCGGCAGCG CGTTTTTCAA TAACGCGGTT CCCCACCGAC CACAGTCCCC GCCTGCAGAG
    GCTGTGGTTC CCGAGCAGCA GCCCCCACCC GTGCTGCAGG AGGGCCACAG TCCCCTGCGG
    GCCCTCGCAC AGCCCGGCCC CATCCTGCCT TCACCTCTGA ACAGCTTCCC GGACGAGAGC
    CCCCCGGGCC TGCCGATGGG GGAGGCTTTG GATCGTATGC ACGGGAGTGT GGCTCTGGAA
    ACCCTGAGGC AGCAGCATGT GCGGCTACAG CAGTGGAGCG AGCACCACGC CTATCTCAGT
    CAGGGCAGCC TCCCGTACCC GCACCATCAC CACCCTCACC CACACCTCCA GCACCTTCCC
    CAGCCGCCCG TTGGATTGCA TCAGCCCCCA GTGAGAGCAG ACTGGAAACT CCCCAGCAGT
    GCCGAAGATG AAGCAGAAAC AACTGACTCA CGGTTTCAAG ACTTACTCAG AGAACTGTCT
    AATCGTGAGC AAAGTGAAAC ACGGGAACTA GCTGAAATGC CACCACCTCA ATCAAGGCTT
    TTGCAATATA GACAAATTCA GACTAGGAGC CCACCAGCAG CCCCCTCGCC CCCGTCCGGC
    GCAGACCACA GCACCCACTT TCCAAACTTC ACTGACAGCA GCAGAGACCT TGAAGTGGCC
    AACAGCCCGG CGTTCCCACA GCGCCTGCCG CCCCCGATGT TTAGCTCTCC TTTCTCGTTG
    CCATCTGAGC ACCTCACCCC TCCTCTGAAA TACCTGGCGC CTGATGGAGC TTGGACTTTT
    GCTAACTTAC AGCAGAATCA CCTGATGGGG CCGAGTTTCC CCTACGGCCT ACCTCCATTG
    CCTCACAGGC CCCCGCAGAA CCCTTTCGTG CAAATGCAGA CCCACCAGCC CGCTGTTGGC
    CAGGAGCCGC TCCAGCCGCT GTCCTCTCGG ACAGTGTCGT CCTCTTCGCT CCCCAGCTTA
    GAAGAGTATG AACCCAGAGG ACCGGGTCGG CCCTTGTACC AAAGAAGAAT CTCATCTAGC
    TCAGTGCAGC CTTGTTCTGA AGAAGCAAGC ACTCCTCAAG ACAGTCTTGC TCAGCGTAAA
    GAGCTTCAGG ACCATAATAA CCAGTCTTCT TTCACCTTTT CCTCCCCGGA GTCCTGGGTA
    AACGCCACCT CATCCACCCC CTATCAGAAC ATTCCGTGCA ATGGATCCAG CAGGCCAGCC
    CAGCCCAGAG AGTTGGCAGC TCCACCCAAG ACCGTCAAAG CCCCCGAGGA ACATCTGAAG
    CCTGAGAGCC TTGAGGGGTC CAGTTCCTTC AATTACAGCG TGCTGCAGCA TCTCGGCCAG
    TTCCCCCCGC TCATGCCCAA CAAGCAGATC GCCGAGTCGG CCAGCAGCAG CAGCCCGCAG
    AGCTCCGGGG GCGGCAAGCC TGCCATGTCC TACGCCAGCG CCCTGCGGGC CCCTCCAAAG
    CCCAGGCCCC CGCCCGAGCA GGCCAAGAAG AGCAGCGACC CTCTGTCTCT GTTTCAAGAA
    CTCAGCCTGG GGAGCTCAGC CGGCAGCAAT GGCTTTTACT CCTATTTTAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836903.1
    CDS152..5965
    Translation

    Target ORF information:

    RefSeq Version XM_024981135.1
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase with zinc finger (HELZ), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981135.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGCAGGA TTCTCATTTG CACCCATTCT
    AATAGTGCTG CTGATCTCTA CATAAAGGAT TATTTACATC CATATGTAGA AGCAGGCAAT
    CCCCAGGCAA GACCTCTCAG GGTATATTTC AGAAACCGCT GGGTGAAGAC TGTCCACCCA
    GTTGTGCATC AGTACTGTTT GATCTCAAGC GCACATTCCA CCTTTCAGAT GCCACAGAAA
    GAAGATATTC TGAAACACCG AGTGGTGGTG GTTACATTGA ACACTTCCCA GTACCTCTGT
    CAGCTGGACC TCGAACCTGG GTTTTTTACT CACATCTTGT TAGACGAGGC TGCCCAGGCC
    ATGGAGTGTG AAACCATCAT GCCTCTGGCA CTAGCAACTA GACACACGCG GGTTGTCCTG
    GCTGGCGACC ACATGCAGCT CAGTCCTTTT GTTTACAGCG AGTTTGCCAG GGAGAGGAAC
    CTTCACGTGT CGTTACTTGA TCGGCTCTAT GAGCACTACC CTGCCGAGTT TCCCTGTAGG
    ATCCTCCTGT GCGAGAACTA CCGTTCCCAC GAAGCTATCA TCAATTACAC CTCTGAGCTC
    TTCTATGAGG GCAAGCTGAT GGCCAGTGGA AAGCAACCAG CACACAAGGA GTTCTATCCA
    CTAACCTTCT TTACAGCACG AGGGGAAGAT GTACAGGAGA AAAATAGCAC AGCTTTTTAT
    AATAATGCAG AGGTATTTGA AGTGGTGGAG CGAGTGGAAG AGCTGAGAAG GAAGTGGCCA
    GTAGCGTGGG GCAAGTTGGA TGATGGAAGT ATTGGTGTGG TGACTCCATA TGCAGACCAG
    GTGTTCAGGA TACGCGCCGA ACTTCGAAAA AAGAGGTTAT CTGACGTTAA CGTAGAAAGG
    GTGCTCAATG TTCAAGGAAA GCAATTCAGA GTTTTGTTTC TTAGCACAGT ACGTACAAGG
    CATACTTGTA AACATAAACA GACACCAATT AAAAAGAAGG AGCAGCTGCT AGAAGACTCC
    ACAGAGGACT TGGATTATGG CTTTTTGTCT AACTACAAGC TCCTCAACAC CGCCATCACA
    AGAGCACAGT CCCTGGTTGC CGTGGTGGGG GACCCCATCG CTCTGTGCTC CATTGGGAGA
    TGCAGGAAAT TTTGGGAGCG GTTTATTGCC CTGTGTCATG AAAACAATAG CCTGCATGGA
    ATCACTTTTG AACAGATCAA AGCGCAGCTA GAGGCTTTAG AACTAAAGAA GACATACGTG
    TTGAATCCAC TGGCACCTGA ATTTATACCC CGGGCTCTAA GAGCACAGCA CGCGGGAAAC
    ACCAACAAAC CGCAGCAATC ACCCCCCAAG GGAAAAAGCC TCCATCATAC CCAGAATGAT
    CATTTCCAGA ATGATGGAAT TGTTCAGCCC AATCCCTCTG TGCTTATTGG CAATCCTATC
    AGAGCATATA CTCCTCCACC TCCTCTTGGA CCTCACCCAA ATTTGGGGAA ATCTCCAAGT
    CCTGTTCAAA GAATCGATCC TCACACTGGG ACGAGTATTC TTTATGTACC TGCTGTCTAT
    GGAGGGAATG TCGTTATGTC GGTGCCTGTA CCTGTGCCAT GGACAGGATA CCAGGGCAGG
    TTTGCGGTTG ATCCTCGAAT CATTACACAC CAGGCAGCAA TGGCCTACAA CATGAACCTC
    TTACAGACAC ATGGACGTGG GTCTCCTATT CCTTATGGCC TTGGGCACCA CCCACCTGTC
    AGCATAGGGC AGCCACAGAA TCAGCATCAG GAGAAGGATC AACATGAGCA AAGTCGCAAC
    GGTAAAAGTG ATACAAATAA TCCTGGACCT GAAATTAATA AGATTCGAAC ACCAGAGAAA
    AAACCTACAG AAGCAAAACA GATTGATTTG GAATCAAACC CACAGAACAG AAGTCCTGAA
    TCACGTTCTG GCATTGTTTA TCCCCATCCC AAATTTCATC GCAAAGATAA TCTCACCCCA
    AGACACATAA ACCTTCCTCT CCCTGCCCCC CATGCCCAGT ATGCCATCCC CGGTCGTCAT
    TTTCATCCCC TCCCCCAGCT CCCGAGACCA CCCTTCCCAA TTCCGCAGCA GCACGCCTTG
    CTGAATCAGC AGCAGAGTAA CTTGCCTGAA CAGCCGAGCC CCGTGCCAGC CCAGCCCAGT
    CAGGTGGCCC CTCAGCTGGG CCCGCCGCCG CCTCAGCTCT CCCCTGCCTA CCCGGCTGGC
    CCCGGCAGCG CGTTTTTCAA TAACGCGGTT CCCCACCGAC CACAGTCCCC GCCTGCAGAG
    GCTGTGGTTC CCGAGCAGCA GCCCCCACCC GTGCTGCAGG AGGGCCACAG TCCCCTGCGG
    GCCCTCGCAC AGCCCGGCCC CATCCTGCCT TCACCTCTGA ACAGCTTCCC GGACGAGAGC
    CCCCCGGGCC TGCCGATGGG GGAGGCTTTG GATCGTATGC ACGGGAGTGT GGCTCTGGAA
    ACCCTGAGGC AGCAGCATGT GCGGCTACAG CAGTGGAGCG AGCACCACGC CTATCTCAGT
    CAGGGCAGCC TCCCGTACCC GCACCATCAC CACCCTCACC CACACCTCCA GCACCTTCCC
    CAGCCGCCCG TTGGATTGCA TCAGCCCCCA GTGAGAGCAG ACTGGAAACT CCCCAGCAGT
    GCCGAAGATG AAGCAGAAAC AACTGACTCA CGGTTTCAAG ACTTACTCAG AGAACTGTCT
    AATCGTGAGC AAAGTGAAAC ACGGGAACTA GCTGAAATGC CACCACCTCA ATCAAGGCTT
    TTGCAATATA GACAAATTCA GACTAGGAGC CCACCAGCAG CCCCCTCGCC CCCGTCCGGC
    GCAGACCACA GCACCCACTT TCCAAACTTC ACTGACAGCA GCAGAGACCT TGAAGTGGCC
    AACAGCCCGG CGTTCCCACA GCGCCTGCCG CCCCCGATGT TTAGCTCTCC TTTCTCGTTG
    CCATCTGAGC ACCTCACCCC TCCTCTGAAA TACCTGGCGC CTGATGGAGC TTGGACTTTT
    GCTAACTTAC AGCAGAATCA CCTGATGGGG CCGAGTTTCC CCTACGGCCT ACCTCCATTG
    CCTCACAGGC CCCCGCAGAA CCCTTTCGTG CAAATGCAGA CCCACCAGCC CGCTGTTGGC
    CAGGAGCCGC TCCAGCCGCT GTCCTCTCGG ACAGTGTCGT CCTCTTCGCT CCCCAGCTTA
    GAAGAGTATG AACCCAGAGG ACCGGGTCGG CCCTTGTACC AAAGAAGAAT CTCATCTAGC
    TCAGTGCAGC CTTGTTCTGA AGAAGCAAGC ACTCCTCAAG ACAGTCTTGC TCAGCGTAAA
    GAGCTTCAGG ACCATAATAA CCAGTCTTCT TTCACCTTTT CCTCCCCGGA GTCCTGGGTA
    AACGCCACCT CATCCACCCC CTATCAGAAC ATTCCGTGCA ATGGATCCAG CAGGCCAGCC
    CAGCCCAGAG AGTTGGCAGC TCCACCCAAG ACCGTCAAAG CCCCCGAGGA ACATCTGAAG
    CCTGAGAGCC TTGAGGGGTC CAGTTCCTTC AATTACAGCG TGCTGCAGCA TCTCGGCCAG
    TTCCCCCCGC TCATGCCCAA CAAGCAGATC GCCGAGTCGG CCAGCAGCAG CAGCCCGCAG
    AGCTCCGGGG GCGGCAAGCC TGCCATGTCC TACGCCAGCG CCCTGCGGGC CCCTCCAAAG
    CCCAGGCCCC CGCCCGAGCA GGCCAAGAAG AGCAGCGACC CTCTGTCTCT GTTTCAAGAA
    CTCAGCCTGG GGAGCTCAGC CGGCAGCAAT GGCTTTTACT CCTATTTTAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227074
    Clone ID Related Accession (Same CDS sequence) XM_024981135.1 , XM_024981134.1 , XM_024981131.1 , XM_024981133.1 , XM_024981132.1
    Accession Version XM_024981134.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable helicase with zinc finger domain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGCAGGA TTCTCATTTG CACCCATTCT
    AATAGTGCTG CTGATCTCTA CATAAAGGAT TATTTACATC CATATGTAGA AGCAGGCAAT
    CCCCAGGCAA GACCTCTCAG GGTATATTTC AGAAACCGCT GGGTGAAGAC TGTCCACCCA
    GTTGTGCATC AGTACTGTTT GATCTCAAGC GCACATTCCA CCTTTCAGAT GCCACAGAAA
    GAAGATATTC TGAAACACCG AGTGGTGGTG GTTACATTGA ACACTTCCCA GTACCTCTGT
    CAGCTGGACC TCGAACCTGG GTTTTTTACT CACATCTTGT TAGACGAGGC TGCCCAGGCC
    ATGGAGTGTG AAACCATCAT GCCTCTGGCA CTAGCAACTA GACACACGCG GGTTGTCCTG
    GCTGGCGACC ACATGCAGCT CAGTCCTTTT GTTTACAGCG AGTTTGCCAG GGAGAGGAAC
    CTTCACGTGT CGTTACTTGA TCGGCTCTAT GAGCACTACC CTGCCGAGTT TCCCTGTAGG
    ATCCTCCTGT GCGAGAACTA CCGTTCCCAC GAAGCTATCA TCAATTACAC CTCTGAGCTC
    TTCTATGAGG GCAAGCTGAT GGCCAGTGGA AAGCAACCAG CACACAAGGA GTTCTATCCA
    CTAACCTTCT TTACAGCACG AGGGGAAGAT GTACAGGAGA AAAATAGCAC AGCTTTTTAT
    AATAATGCAG AGGTATTTGA AGTGGTGGAG CGAGTGGAAG AGCTGAGAAG GAAGTGGCCA
    GTAGCGTGGG GCAAGTTGGA TGATGGAAGT ATTGGTGTGG TGACTCCATA TGCAGACCAG
    GTGTTCAGGA TACGCGCCGA ACTTCGAAAA AAGAGGTTAT CTGACGTTAA CGTAGAAAGG
    GTGCTCAATG TTCAAGGAAA GCAATTCAGA GTTTTGTTTC TTAGCACAGT ACGTACAAGG
    CATACTTGTA AACATAAACA GACACCAATT AAAAAGAAGG AGCAGCTGCT AGAAGACTCC
    ACAGAGGACT TGGATTATGG CTTTTTGTCT AACTACAAGC TCCTCAACAC CGCCATCACA
    AGAGCACAGT CCCTGGTTGC CGTGGTGGGG GACCCCATCG CTCTGTGCTC CATTGGGAGA
    TGCAGGAAAT TTTGGGAGCG GTTTATTGCC CTGTGTCATG AAAACAATAG CCTGCATGGA
    ATCACTTTTG AACAGATCAA AGCGCAGCTA GAGGCTTTAG AACTAAAGAA GACATACGTG
    TTGAATCCAC TGGCACCTGA ATTTATACCC CGGGCTCTAA GAGCACAGCA CGCGGGAAAC
    ACCAACAAAC CGCAGCAATC ACCCCCCAAG GGAAAAAGCC TCCATCATAC CCAGAATGAT
    CATTTCCAGA ATGATGGAAT TGTTCAGCCC AATCCCTCTG TGCTTATTGG CAATCCTATC
    AGAGCATATA CTCCTCCACC TCCTCTTGGA CCTCACCCAA ATTTGGGGAA ATCTCCAAGT
    CCTGTTCAAA GAATCGATCC TCACACTGGG ACGAGTATTC TTTATGTACC TGCTGTCTAT
    GGAGGGAATG TCGTTATGTC GGTGCCTGTA CCTGTGCCAT GGACAGGATA CCAGGGCAGG
    TTTGCGGTTG ATCCTCGAAT CATTACACAC CAGGCAGCAA TGGCCTACAA CATGAACCTC
    TTACAGACAC ATGGACGTGG GTCTCCTATT CCTTATGGCC TTGGGCACCA CCCACCTGTC
    AGCATAGGGC AGCCACAGAA TCAGCATCAG GAGAAGGATC AACATGAGCA AAGTCGCAAC
    GGTAAAAGTG ATACAAATAA TCCTGGACCT GAAATTAATA AGATTCGAAC ACCAGAGAAA
    AAACCTACAG AAGCAAAACA GATTGATTTG GAATCAAACC CACAGAACAG AAGTCCTGAA
    TCACGTTCTG GCATTGTTTA TCCCCATCCC AAATTTCATC GCAAAGATAA TCTCACCCCA
    AGACACATAA ACCTTCCTCT CCCTGCCCCC CATGCCCAGT ATGCCATCCC CGGTCGTCAT
    TTTCATCCCC TCCCCCAGCT CCCGAGACCA CCCTTCCCAA TTCCGCAGCA GCACGCCTTG
    CTGAATCAGC AGCAGAGTAA CTTGCCTGAA CAGCCGAGCC CCGTGCCAGC CCAGCCCAGT
    CAGGTGGCCC CTCAGCTGGG CCCGCCGCCG CCTCAGCTCT CCCCTGCCTA CCCGGCTGGC
    CCCGGCAGCG CGTTTTTCAA TAACGCGGTT CCCCACCGAC CACAGTCCCC GCCTGCAGAG
    GCTGTGGTTC CCGAGCAGCA GCCCCCACCC GTGCTGCAGG AGGGCCACAG TCCCCTGCGG
    GCCCTCGCAC AGCCCGGCCC CATCCTGCCT TCACCTCTGA ACAGCTTCCC GGACGAGAGC
    CCCCCGGGCC TGCCGATGGG GGAGGCTTTG GATCGTATGC ACGGGAGTGT GGCTCTGGAA
    ACCCTGAGGC AGCAGCATGT GCGGCTACAG CAGTGGAGCG AGCACCACGC CTATCTCAGT
    CAGGGCAGCC TCCCGTACCC GCACCATCAC CACCCTCACC CACACCTCCA GCACCTTCCC
    CAGCCGCCCG TTGGATTGCA TCAGCCCCCA GTGAGAGCAG ACTGGAAACT CCCCAGCAGT
    GCCGAAGATG AAGCAGAAAC AACTGACTCA CGGTTTCAAG ACTTACTCAG AGAACTGTCT
    AATCGTGAGC AAAGTGAAAC ACGGGAACTA GCTGAAATGC CACCACCTCA ATCAAGGCTT
    TTGCAATATA GACAAATTCA GACTAGGAGC CCACCAGCAG CCCCCTCGCC CCCGTCCGGC
    GCAGACCACA GCACCCACTT TCCAAACTTC ACTGACAGCA GCAGAGACCT TGAAGTGGCC
    AACAGCCCGG CGTTCCCACA GCGCCTGCCG CCCCCGATGT TTAGCTCTCC TTTCTCGTTG
    CCATCTGAGC ACCTCACCCC TCCTCTGAAA TACCTGGCGC CTGATGGAGC TTGGACTTTT
    GCTAACTTAC AGCAGAATCA CCTGATGGGG CCGAGTTTCC CCTACGGCCT ACCTCCATTG
    CCTCACAGGC CCCCGCAGAA CCCTTTCGTG CAAATGCAGA CCCACCAGCC CGCTGTTGGC
    CAGGAGCCGC TCCAGCCGCT GTCCTCTCGG ACAGTGTCGT CCTCTTCGCT CCCCAGCTTA
    GAAGAGTATG AACCCAGAGG ACCGGGTCGG CCCTTGTACC AAAGAAGAAT CTCATCTAGC
    TCAGTGCAGC CTTGTTCTGA AGAAGCAAGC ACTCCTCAAG ACAGTCTTGC TCAGCGTAAA
    GAGCTTCAGG ACCATAATAA CCAGTCTTCT TTCACCTTTT CCTCCCCGGA GTCCTGGGTA
    AACGCCACCT CATCCACCCC CTATCAGAAC ATTCCGTGCA ATGGATCCAG CAGGCCAGCC
    CAGCCCAGAG AGTTGGCAGC TCCACCCAAG ACCGTCAAAG CCCCCGAGGA ACATCTGAAG
    CCTGAGAGCC TTGAGGGGTC CAGTTCCTTC AATTACAGCG TGCTGCAGCA TCTCGGCCAG
    TTCCCCCCGC TCATGCCCAA CAAGCAGATC GCCGAGTCGG CCAGCAGCAG CAGCCCGCAG
    AGCTCCGGGG GCGGCAAGCC TGCCATGTCC TACGCCAGCG CCCTGCGGGC CCCTCCAAAG
    CCCAGGCCCC CGCCCGAGCA GGCCAAGAAG AGCAGCGACC CTCTGTCTCT GTTTCAAGAA
    CTCAGCCTGG GGAGCTCAGC CGGCAGCAAT GGCTTTTACT CCTATTTTAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836902.1
    CDS161..5974
    Translation

    Target ORF information:

    RefSeq Version XM_024981134.1
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase with zinc finger (HELZ), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981134.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGCAGGA TTCTCATTTG CACCCATTCT
    AATAGTGCTG CTGATCTCTA CATAAAGGAT TATTTACATC CATATGTAGA AGCAGGCAAT
    CCCCAGGCAA GACCTCTCAG GGTATATTTC AGAAACCGCT GGGTGAAGAC TGTCCACCCA
    GTTGTGCATC AGTACTGTTT GATCTCAAGC GCACATTCCA CCTTTCAGAT GCCACAGAAA
    GAAGATATTC TGAAACACCG AGTGGTGGTG GTTACATTGA ACACTTCCCA GTACCTCTGT
    CAGCTGGACC TCGAACCTGG GTTTTTTACT CACATCTTGT TAGACGAGGC TGCCCAGGCC
    ATGGAGTGTG AAACCATCAT GCCTCTGGCA CTAGCAACTA GACACACGCG GGTTGTCCTG
    GCTGGCGACC ACATGCAGCT CAGTCCTTTT GTTTACAGCG AGTTTGCCAG GGAGAGGAAC
    CTTCACGTGT CGTTACTTGA TCGGCTCTAT GAGCACTACC CTGCCGAGTT TCCCTGTAGG
    ATCCTCCTGT GCGAGAACTA CCGTTCCCAC GAAGCTATCA TCAATTACAC CTCTGAGCTC
    TTCTATGAGG GCAAGCTGAT GGCCAGTGGA AAGCAACCAG CACACAAGGA GTTCTATCCA
    CTAACCTTCT TTACAGCACG AGGGGAAGAT GTACAGGAGA AAAATAGCAC AGCTTTTTAT
    AATAATGCAG AGGTATTTGA AGTGGTGGAG CGAGTGGAAG AGCTGAGAAG GAAGTGGCCA
    GTAGCGTGGG GCAAGTTGGA TGATGGAAGT ATTGGTGTGG TGACTCCATA TGCAGACCAG
    GTGTTCAGGA TACGCGCCGA ACTTCGAAAA AAGAGGTTAT CTGACGTTAA CGTAGAAAGG
    GTGCTCAATG TTCAAGGAAA GCAATTCAGA GTTTTGTTTC TTAGCACAGT ACGTACAAGG
    CATACTTGTA AACATAAACA GACACCAATT AAAAAGAAGG AGCAGCTGCT AGAAGACTCC
    ACAGAGGACT TGGATTATGG CTTTTTGTCT AACTACAAGC TCCTCAACAC CGCCATCACA
    AGAGCACAGT CCCTGGTTGC CGTGGTGGGG GACCCCATCG CTCTGTGCTC CATTGGGAGA
    TGCAGGAAAT TTTGGGAGCG GTTTATTGCC CTGTGTCATG AAAACAATAG CCTGCATGGA
    ATCACTTTTG AACAGATCAA AGCGCAGCTA GAGGCTTTAG AACTAAAGAA GACATACGTG
    TTGAATCCAC TGGCACCTGA ATTTATACCC CGGGCTCTAA GAGCACAGCA CGCGGGAAAC
    ACCAACAAAC CGCAGCAATC ACCCCCCAAG GGAAAAAGCC TCCATCATAC CCAGAATGAT
    CATTTCCAGA ATGATGGAAT TGTTCAGCCC AATCCCTCTG TGCTTATTGG CAATCCTATC
    AGAGCATATA CTCCTCCACC TCCTCTTGGA CCTCACCCAA ATTTGGGGAA ATCTCCAAGT
    CCTGTTCAAA GAATCGATCC TCACACTGGG ACGAGTATTC TTTATGTACC TGCTGTCTAT
    GGAGGGAATG TCGTTATGTC GGTGCCTGTA CCTGTGCCAT GGACAGGATA CCAGGGCAGG
    TTTGCGGTTG ATCCTCGAAT CATTACACAC CAGGCAGCAA TGGCCTACAA CATGAACCTC
    TTACAGACAC ATGGACGTGG GTCTCCTATT CCTTATGGCC TTGGGCACCA CCCACCTGTC
    AGCATAGGGC AGCCACAGAA TCAGCATCAG GAGAAGGATC AACATGAGCA AAGTCGCAAC
    GGTAAAAGTG ATACAAATAA TCCTGGACCT GAAATTAATA AGATTCGAAC ACCAGAGAAA
    AAACCTACAG AAGCAAAACA GATTGATTTG GAATCAAACC CACAGAACAG AAGTCCTGAA
    TCACGTTCTG GCATTGTTTA TCCCCATCCC AAATTTCATC GCAAAGATAA TCTCACCCCA
    AGACACATAA ACCTTCCTCT CCCTGCCCCC CATGCCCAGT ATGCCATCCC CGGTCGTCAT
    TTTCATCCCC TCCCCCAGCT CCCGAGACCA CCCTTCCCAA TTCCGCAGCA GCACGCCTTG
    CTGAATCAGC AGCAGAGTAA CTTGCCTGAA CAGCCGAGCC CCGTGCCAGC CCAGCCCAGT
    CAGGTGGCCC CTCAGCTGGG CCCGCCGCCG CCTCAGCTCT CCCCTGCCTA CCCGGCTGGC
    CCCGGCAGCG CGTTTTTCAA TAACGCGGTT CCCCACCGAC CACAGTCCCC GCCTGCAGAG
    GCTGTGGTTC CCGAGCAGCA GCCCCCACCC GTGCTGCAGG AGGGCCACAG TCCCCTGCGG
    GCCCTCGCAC AGCCCGGCCC CATCCTGCCT TCACCTCTGA ACAGCTTCCC GGACGAGAGC
    CCCCCGGGCC TGCCGATGGG GGAGGCTTTG GATCGTATGC ACGGGAGTGT GGCTCTGGAA
    ACCCTGAGGC AGCAGCATGT GCGGCTACAG CAGTGGAGCG AGCACCACGC CTATCTCAGT
    CAGGGCAGCC TCCCGTACCC GCACCATCAC CACCCTCACC CACACCTCCA GCACCTTCCC
    CAGCCGCCCG TTGGATTGCA TCAGCCCCCA GTGAGAGCAG ACTGGAAACT CCCCAGCAGT
    GCCGAAGATG AAGCAGAAAC AACTGACTCA CGGTTTCAAG ACTTACTCAG AGAACTGTCT
    AATCGTGAGC AAAGTGAAAC ACGGGAACTA GCTGAAATGC CACCACCTCA ATCAAGGCTT
    TTGCAATATA GACAAATTCA GACTAGGAGC CCACCAGCAG CCCCCTCGCC CCCGTCCGGC
    GCAGACCACA GCACCCACTT TCCAAACTTC ACTGACAGCA GCAGAGACCT TGAAGTGGCC
    AACAGCCCGG CGTTCCCACA GCGCCTGCCG CCCCCGATGT TTAGCTCTCC TTTCTCGTTG
    CCATCTGAGC ACCTCACCCC TCCTCTGAAA TACCTGGCGC CTGATGGAGC TTGGACTTTT
    GCTAACTTAC AGCAGAATCA CCTGATGGGG CCGAGTTTCC CCTACGGCCT ACCTCCATTG
    CCTCACAGGC CCCCGCAGAA CCCTTTCGTG CAAATGCAGA CCCACCAGCC CGCTGTTGGC
    CAGGAGCCGC TCCAGCCGCT GTCCTCTCGG ACAGTGTCGT CCTCTTCGCT CCCCAGCTTA
    GAAGAGTATG AACCCAGAGG ACCGGGTCGG CCCTTGTACC AAAGAAGAAT CTCATCTAGC
    TCAGTGCAGC CTTGTTCTGA AGAAGCAAGC ACTCCTCAAG ACAGTCTTGC TCAGCGTAAA
    GAGCTTCAGG ACCATAATAA CCAGTCTTCT TTCACCTTTT CCTCCCCGGA GTCCTGGGTA
    AACGCCACCT CATCCACCCC CTATCAGAAC ATTCCGTGCA ATGGATCCAG CAGGCCAGCC
    CAGCCCAGAG AGTTGGCAGC TCCACCCAAG ACCGTCAAAG CCCCCGAGGA ACATCTGAAG
    CCTGAGAGCC TTGAGGGGTC CAGTTCCTTC AATTACAGCG TGCTGCAGCA TCTCGGCCAG
    TTCCCCCCGC TCATGCCCAA CAAGCAGATC GCCGAGTCGG CCAGCAGCAG CAGCCCGCAG
    AGCTCCGGGG GCGGCAAGCC TGCCATGTCC TACGCCAGCG CCCTGCGGGC CCCTCCAAAG
    CCCAGGCCCC CGCCCGAGCA GGCCAAGAAG AGCAGCGACC CTCTGTCTCT GTTTCAAGAA
    CTCAGCCTGG GGAGCTCAGC CGGCAGCAAT GGCTTTTACT CCTATTTTAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227074
    Clone ID Related Accession (Same CDS sequence) XM_024981135.1 , XM_024981134.1 , XM_024981131.1 , XM_024981133.1 , XM_024981132.1
    Accession Version XM_024981131.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable helicase with zinc finger domain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGCAGGA TTCTCATTTG CACCCATTCT
    AATAGTGCTG CTGATCTCTA CATAAAGGAT TATTTACATC CATATGTAGA AGCAGGCAAT
    CCCCAGGCAA GACCTCTCAG GGTATATTTC AGAAACCGCT GGGTGAAGAC TGTCCACCCA
    GTTGTGCATC AGTACTGTTT GATCTCAAGC GCACATTCCA CCTTTCAGAT GCCACAGAAA
    GAAGATATTC TGAAACACCG AGTGGTGGTG GTTACATTGA ACACTTCCCA GTACCTCTGT
    CAGCTGGACC TCGAACCTGG GTTTTTTACT CACATCTTGT TAGACGAGGC TGCCCAGGCC
    ATGGAGTGTG AAACCATCAT GCCTCTGGCA CTAGCAACTA GACACACGCG GGTTGTCCTG
    GCTGGCGACC ACATGCAGCT CAGTCCTTTT GTTTACAGCG AGTTTGCCAG GGAGAGGAAC
    CTTCACGTGT CGTTACTTGA TCGGCTCTAT GAGCACTACC CTGCCGAGTT TCCCTGTAGG
    ATCCTCCTGT GCGAGAACTA CCGTTCCCAC GAAGCTATCA TCAATTACAC CTCTGAGCTC
    TTCTATGAGG GCAAGCTGAT GGCCAGTGGA AAGCAACCAG CACACAAGGA GTTCTATCCA
    CTAACCTTCT TTACAGCACG AGGGGAAGAT GTACAGGAGA AAAATAGCAC AGCTTTTTAT
    AATAATGCAG AGGTATTTGA AGTGGTGGAG CGAGTGGAAG AGCTGAGAAG GAAGTGGCCA
    GTAGCGTGGG GCAAGTTGGA TGATGGAAGT ATTGGTGTGG TGACTCCATA TGCAGACCAG
    GTGTTCAGGA TACGCGCCGA ACTTCGAAAA AAGAGGTTAT CTGACGTTAA CGTAGAAAGG
    GTGCTCAATG TTCAAGGAAA GCAATTCAGA GTTTTGTTTC TTAGCACAGT ACGTACAAGG
    CATACTTGTA AACATAAACA GACACCAATT AAAAAGAAGG AGCAGCTGCT AGAAGACTCC
    ACAGAGGACT TGGATTATGG CTTTTTGTCT AACTACAAGC TCCTCAACAC CGCCATCACA
    AGAGCACAGT CCCTGGTTGC CGTGGTGGGG GACCCCATCG CTCTGTGCTC CATTGGGAGA
    TGCAGGAAAT TTTGGGAGCG GTTTATTGCC CTGTGTCATG AAAACAATAG CCTGCATGGA
    ATCACTTTTG AACAGATCAA AGCGCAGCTA GAGGCTTTAG AACTAAAGAA GACATACGTG
    TTGAATCCAC TGGCACCTGA ATTTATACCC CGGGCTCTAA GAGCACAGCA CGCGGGAAAC
    ACCAACAAAC CGCAGCAATC ACCCCCCAAG GGAAAAAGCC TCCATCATAC CCAGAATGAT
    CATTTCCAGA ATGATGGAAT TGTTCAGCCC AATCCCTCTG TGCTTATTGG CAATCCTATC
    AGAGCATATA CTCCTCCACC TCCTCTTGGA CCTCACCCAA ATTTGGGGAA ATCTCCAAGT
    CCTGTTCAAA GAATCGATCC TCACACTGGG ACGAGTATTC TTTATGTACC TGCTGTCTAT
    GGAGGGAATG TCGTTATGTC GGTGCCTGTA CCTGTGCCAT GGACAGGATA CCAGGGCAGG
    TTTGCGGTTG ATCCTCGAAT CATTACACAC CAGGCAGCAA TGGCCTACAA CATGAACCTC
    TTACAGACAC ATGGACGTGG GTCTCCTATT CCTTATGGCC TTGGGCACCA CCCACCTGTC
    AGCATAGGGC AGCCACAGAA TCAGCATCAG GAGAAGGATC AACATGAGCA AAGTCGCAAC
    GGTAAAAGTG ATACAAATAA TCCTGGACCT GAAATTAATA AGATTCGAAC ACCAGAGAAA
    AAACCTACAG AAGCAAAACA GATTGATTTG GAATCAAACC CACAGAACAG AAGTCCTGAA
    TCACGTTCTG GCATTGTTTA TCCCCATCCC AAATTTCATC GCAAAGATAA TCTCACCCCA
    AGACACATAA ACCTTCCTCT CCCTGCCCCC CATGCCCAGT ATGCCATCCC CGGTCGTCAT
    TTTCATCCCC TCCCCCAGCT CCCGAGACCA CCCTTCCCAA TTCCGCAGCA GCACGCCTTG
    CTGAATCAGC AGCAGAGTAA CTTGCCTGAA CAGCCGAGCC CCGTGCCAGC CCAGCCCAGT
    CAGGTGGCCC CTCAGCTGGG CCCGCCGCCG CCTCAGCTCT CCCCTGCCTA CCCGGCTGGC
    CCCGGCAGCG CGTTTTTCAA TAACGCGGTT CCCCACCGAC CACAGTCCCC GCCTGCAGAG
    GCTGTGGTTC CCGAGCAGCA GCCCCCACCC GTGCTGCAGG AGGGCCACAG TCCCCTGCGG
    GCCCTCGCAC AGCCCGGCCC CATCCTGCCT TCACCTCTGA ACAGCTTCCC GGACGAGAGC
    CCCCCGGGCC TGCCGATGGG GGAGGCTTTG GATCGTATGC ACGGGAGTGT GGCTCTGGAA
    ACCCTGAGGC AGCAGCATGT GCGGCTACAG CAGTGGAGCG AGCACCACGC CTATCTCAGT
    CAGGGCAGCC TCCCGTACCC GCACCATCAC CACCCTCACC CACACCTCCA GCACCTTCCC
    CAGCCGCCCG TTGGATTGCA TCAGCCCCCA GTGAGAGCAG ACTGGAAACT CCCCAGCAGT
    GCCGAAGATG AAGCAGAAAC AACTGACTCA CGGTTTCAAG ACTTACTCAG AGAACTGTCT
    AATCGTGAGC AAAGTGAAAC ACGGGAACTA GCTGAAATGC CACCACCTCA ATCAAGGCTT
    TTGCAATATA GACAAATTCA GACTAGGAGC CCACCAGCAG CCCCCTCGCC CCCGTCCGGC
    GCAGACCACA GCACCCACTT TCCAAACTTC ACTGACAGCA GCAGAGACCT TGAAGTGGCC
    AACAGCCCGG CGTTCCCACA GCGCCTGCCG CCCCCGATGT TTAGCTCTCC TTTCTCGTTG
    CCATCTGAGC ACCTCACCCC TCCTCTGAAA TACCTGGCGC CTGATGGAGC TTGGACTTTT
    GCTAACTTAC AGCAGAATCA CCTGATGGGG CCGAGTTTCC CCTACGGCCT ACCTCCATTG
    CCTCACAGGC CCCCGCAGAA CCCTTTCGTG CAAATGCAGA CCCACCAGCC CGCTGTTGGC
    CAGGAGCCGC TCCAGCCGCT GTCCTCTCGG ACAGTGTCGT CCTCTTCGCT CCCCAGCTTA
    GAAGAGTATG AACCCAGAGG ACCGGGTCGG CCCTTGTACC AAAGAAGAAT CTCATCTAGC
    TCAGTGCAGC CTTGTTCTGA AGAAGCAAGC ACTCCTCAAG ACAGTCTTGC TCAGCGTAAA
    GAGCTTCAGG ACCATAATAA CCAGTCTTCT TTCACCTTTT CCTCCCCGGA GTCCTGGGTA
    AACGCCACCT CATCCACCCC CTATCAGAAC ATTCCGTGCA ATGGATCCAG CAGGCCAGCC
    CAGCCCAGAG AGTTGGCAGC TCCACCCAAG ACCGTCAAAG CCCCCGAGGA ACATCTGAAG
    CCTGAGAGCC TTGAGGGGTC CAGTTCCTTC AATTACAGCG TGCTGCAGCA TCTCGGCCAG
    TTCCCCCCGC TCATGCCCAA CAAGCAGATC GCCGAGTCGG CCAGCAGCAG CAGCCCGCAG
    AGCTCCGGGG GCGGCAAGCC TGCCATGTCC TACGCCAGCG CCCTGCGGGC CCCTCCAAAG
    CCCAGGCCCC CGCCCGAGCA GGCCAAGAAG AGCAGCGACC CTCTGTCTCT GTTTCAAGAA
    CTCAGCCTGG GGAGCTCAGC CGGCAGCAAT GGCTTTTACT CCTATTTTAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836899.1
    CDS264..6077
    Translation

    Target ORF information:

    RefSeq Version XM_024981131.1
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase with zinc finger (HELZ), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981131.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGCAGGA TTCTCATTTG CACCCATTCT
    AATAGTGCTG CTGATCTCTA CATAAAGGAT TATTTACATC CATATGTAGA AGCAGGCAAT
    CCCCAGGCAA GACCTCTCAG GGTATATTTC AGAAACCGCT GGGTGAAGAC TGTCCACCCA
    GTTGTGCATC AGTACTGTTT GATCTCAAGC GCACATTCCA CCTTTCAGAT GCCACAGAAA
    GAAGATATTC TGAAACACCG AGTGGTGGTG GTTACATTGA ACACTTCCCA GTACCTCTGT
    CAGCTGGACC TCGAACCTGG GTTTTTTACT CACATCTTGT TAGACGAGGC TGCCCAGGCC
    ATGGAGTGTG AAACCATCAT GCCTCTGGCA CTAGCAACTA GACACACGCG GGTTGTCCTG
    GCTGGCGACC ACATGCAGCT CAGTCCTTTT GTTTACAGCG AGTTTGCCAG GGAGAGGAAC
    CTTCACGTGT CGTTACTTGA TCGGCTCTAT GAGCACTACC CTGCCGAGTT TCCCTGTAGG
    ATCCTCCTGT GCGAGAACTA CCGTTCCCAC GAAGCTATCA TCAATTACAC CTCTGAGCTC
    TTCTATGAGG GCAAGCTGAT GGCCAGTGGA AAGCAACCAG CACACAAGGA GTTCTATCCA
    CTAACCTTCT TTACAGCACG AGGGGAAGAT GTACAGGAGA AAAATAGCAC AGCTTTTTAT
    AATAATGCAG AGGTATTTGA AGTGGTGGAG CGAGTGGAAG AGCTGAGAAG GAAGTGGCCA
    GTAGCGTGGG GCAAGTTGGA TGATGGAAGT ATTGGTGTGG TGACTCCATA TGCAGACCAG
    GTGTTCAGGA TACGCGCCGA ACTTCGAAAA AAGAGGTTAT CTGACGTTAA CGTAGAAAGG
    GTGCTCAATG TTCAAGGAAA GCAATTCAGA GTTTTGTTTC TTAGCACAGT ACGTACAAGG
    CATACTTGTA AACATAAACA GACACCAATT AAAAAGAAGG AGCAGCTGCT AGAAGACTCC
    ACAGAGGACT TGGATTATGG CTTTTTGTCT AACTACAAGC TCCTCAACAC CGCCATCACA
    AGAGCACAGT CCCTGGTTGC CGTGGTGGGG GACCCCATCG CTCTGTGCTC CATTGGGAGA
    TGCAGGAAAT TTTGGGAGCG GTTTATTGCC CTGTGTCATG AAAACAATAG CCTGCATGGA
    ATCACTTTTG AACAGATCAA AGCGCAGCTA GAGGCTTTAG AACTAAAGAA GACATACGTG
    TTGAATCCAC TGGCACCTGA ATTTATACCC CGGGCTCTAA GAGCACAGCA CGCGGGAAAC
    ACCAACAAAC CGCAGCAATC ACCCCCCAAG GGAAAAAGCC TCCATCATAC CCAGAATGAT
    CATTTCCAGA ATGATGGAAT TGTTCAGCCC AATCCCTCTG TGCTTATTGG CAATCCTATC
    AGAGCATATA CTCCTCCACC TCCTCTTGGA CCTCACCCAA ATTTGGGGAA ATCTCCAAGT
    CCTGTTCAAA GAATCGATCC TCACACTGGG ACGAGTATTC TTTATGTACC TGCTGTCTAT
    GGAGGGAATG TCGTTATGTC GGTGCCTGTA CCTGTGCCAT GGACAGGATA CCAGGGCAGG
    TTTGCGGTTG ATCCTCGAAT CATTACACAC CAGGCAGCAA TGGCCTACAA CATGAACCTC
    TTACAGACAC ATGGACGTGG GTCTCCTATT CCTTATGGCC TTGGGCACCA CCCACCTGTC
    AGCATAGGGC AGCCACAGAA TCAGCATCAG GAGAAGGATC AACATGAGCA AAGTCGCAAC
    GGTAAAAGTG ATACAAATAA TCCTGGACCT GAAATTAATA AGATTCGAAC ACCAGAGAAA
    AAACCTACAG AAGCAAAACA GATTGATTTG GAATCAAACC CACAGAACAG AAGTCCTGAA
    TCACGTTCTG GCATTGTTTA TCCCCATCCC AAATTTCATC GCAAAGATAA TCTCACCCCA
    AGACACATAA ACCTTCCTCT CCCTGCCCCC CATGCCCAGT ATGCCATCCC CGGTCGTCAT
    TTTCATCCCC TCCCCCAGCT CCCGAGACCA CCCTTCCCAA TTCCGCAGCA GCACGCCTTG
    CTGAATCAGC AGCAGAGTAA CTTGCCTGAA CAGCCGAGCC CCGTGCCAGC CCAGCCCAGT
    CAGGTGGCCC CTCAGCTGGG CCCGCCGCCG CCTCAGCTCT CCCCTGCCTA CCCGGCTGGC
    CCCGGCAGCG CGTTTTTCAA TAACGCGGTT CCCCACCGAC CACAGTCCCC GCCTGCAGAG
    GCTGTGGTTC CCGAGCAGCA GCCCCCACCC GTGCTGCAGG AGGGCCACAG TCCCCTGCGG
    GCCCTCGCAC AGCCCGGCCC CATCCTGCCT TCACCTCTGA ACAGCTTCCC GGACGAGAGC
    CCCCCGGGCC TGCCGATGGG GGAGGCTTTG GATCGTATGC ACGGGAGTGT GGCTCTGGAA
    ACCCTGAGGC AGCAGCATGT GCGGCTACAG CAGTGGAGCG AGCACCACGC CTATCTCAGT
    CAGGGCAGCC TCCCGTACCC GCACCATCAC CACCCTCACC CACACCTCCA GCACCTTCCC
    CAGCCGCCCG TTGGATTGCA TCAGCCCCCA GTGAGAGCAG ACTGGAAACT CCCCAGCAGT
    GCCGAAGATG AAGCAGAAAC AACTGACTCA CGGTTTCAAG ACTTACTCAG AGAACTGTCT
    AATCGTGAGC AAAGTGAAAC ACGGGAACTA GCTGAAATGC CACCACCTCA ATCAAGGCTT
    TTGCAATATA GACAAATTCA GACTAGGAGC CCACCAGCAG CCCCCTCGCC CCCGTCCGGC
    GCAGACCACA GCACCCACTT TCCAAACTTC ACTGACAGCA GCAGAGACCT TGAAGTGGCC
    AACAGCCCGG CGTTCCCACA GCGCCTGCCG CCCCCGATGT TTAGCTCTCC TTTCTCGTTG
    CCATCTGAGC ACCTCACCCC TCCTCTGAAA TACCTGGCGC CTGATGGAGC TTGGACTTTT
    GCTAACTTAC AGCAGAATCA CCTGATGGGG CCGAGTTTCC CCTACGGCCT ACCTCCATTG
    CCTCACAGGC CCCCGCAGAA CCCTTTCGTG CAAATGCAGA CCCACCAGCC CGCTGTTGGC
    CAGGAGCCGC TCCAGCCGCT GTCCTCTCGG ACAGTGTCGT CCTCTTCGCT CCCCAGCTTA
    GAAGAGTATG AACCCAGAGG ACCGGGTCGG CCCTTGTACC AAAGAAGAAT CTCATCTAGC
    TCAGTGCAGC CTTGTTCTGA AGAAGCAAGC ACTCCTCAAG ACAGTCTTGC TCAGCGTAAA
    GAGCTTCAGG ACCATAATAA CCAGTCTTCT TTCACCTTTT CCTCCCCGGA GTCCTGGGTA
    AACGCCACCT CATCCACCCC CTATCAGAAC ATTCCGTGCA ATGGATCCAG CAGGCCAGCC
    CAGCCCAGAG AGTTGGCAGC TCCACCCAAG ACCGTCAAAG CCCCCGAGGA ACATCTGAAG
    CCTGAGAGCC TTGAGGGGTC CAGTTCCTTC AATTACAGCG TGCTGCAGCA TCTCGGCCAG
    TTCCCCCCGC TCATGCCCAA CAAGCAGATC GCCGAGTCGG CCAGCAGCAG CAGCCCGCAG
    AGCTCCGGGG GCGGCAAGCC TGCCATGTCC TACGCCAGCG CCCTGCGGGC CCCTCCAAAG
    CCCAGGCCCC CGCCCGAGCA GGCCAAGAAG AGCAGCGACC CTCTGTCTCT GTTTCAAGAA
    CTCAGCCTGG GGAGCTCAGC CGGCAGCAAT GGCTTTTACT CCTATTTTAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227074
    Clone ID Related Accession (Same CDS sequence) XM_024981135.1 , XM_024981134.1 , XM_024981131.1 , XM_024981133.1 , XM_024981132.1
    Accession Version XM_024981133.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable helicase with zinc finger domain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGCAGGA TTCTCATTTG CACCCATTCT
    AATAGTGCTG CTGATCTCTA CATAAAGGAT TATTTACATC CATATGTAGA AGCAGGCAAT
    CCCCAGGCAA GACCTCTCAG GGTATATTTC AGAAACCGCT GGGTGAAGAC TGTCCACCCA
    GTTGTGCATC AGTACTGTTT GATCTCAAGC GCACATTCCA CCTTTCAGAT GCCACAGAAA
    GAAGATATTC TGAAACACCG AGTGGTGGTG GTTACATTGA ACACTTCCCA GTACCTCTGT
    CAGCTGGACC TCGAACCTGG GTTTTTTACT CACATCTTGT TAGACGAGGC TGCCCAGGCC
    ATGGAGTGTG AAACCATCAT GCCTCTGGCA CTAGCAACTA GACACACGCG GGTTGTCCTG
    GCTGGCGACC ACATGCAGCT CAGTCCTTTT GTTTACAGCG AGTTTGCCAG GGAGAGGAAC
    CTTCACGTGT CGTTACTTGA TCGGCTCTAT GAGCACTACC CTGCCGAGTT TCCCTGTAGG
    ATCCTCCTGT GCGAGAACTA CCGTTCCCAC GAAGCTATCA TCAATTACAC CTCTGAGCTC
    TTCTATGAGG GCAAGCTGAT GGCCAGTGGA AAGCAACCAG CACACAAGGA GTTCTATCCA
    CTAACCTTCT TTACAGCACG AGGGGAAGAT GTACAGGAGA AAAATAGCAC AGCTTTTTAT
    AATAATGCAG AGGTATTTGA AGTGGTGGAG CGAGTGGAAG AGCTGAGAAG GAAGTGGCCA
    GTAGCGTGGG GCAAGTTGGA TGATGGAAGT ATTGGTGTGG TGACTCCATA TGCAGACCAG
    GTGTTCAGGA TACGCGCCGA ACTTCGAAAA AAGAGGTTAT CTGACGTTAA CGTAGAAAGG
    GTGCTCAATG TTCAAGGAAA GCAATTCAGA GTTTTGTTTC TTAGCACAGT ACGTACAAGG
    CATACTTGTA AACATAAACA GACACCAATT AAAAAGAAGG AGCAGCTGCT AGAAGACTCC
    ACAGAGGACT TGGATTATGG CTTTTTGTCT AACTACAAGC TCCTCAACAC CGCCATCACA
    AGAGCACAGT CCCTGGTTGC CGTGGTGGGG GACCCCATCG CTCTGTGCTC CATTGGGAGA
    TGCAGGAAAT TTTGGGAGCG GTTTATTGCC CTGTGTCATG AAAACAATAG CCTGCATGGA
    ATCACTTTTG AACAGATCAA AGCGCAGCTA GAGGCTTTAG AACTAAAGAA GACATACGTG
    TTGAATCCAC TGGCACCTGA ATTTATACCC CGGGCTCTAA GAGCACAGCA CGCGGGAAAC
    ACCAACAAAC CGCAGCAATC ACCCCCCAAG GGAAAAAGCC TCCATCATAC CCAGAATGAT
    CATTTCCAGA ATGATGGAAT TGTTCAGCCC AATCCCTCTG TGCTTATTGG CAATCCTATC
    AGAGCATATA CTCCTCCACC TCCTCTTGGA CCTCACCCAA ATTTGGGGAA ATCTCCAAGT
    CCTGTTCAAA GAATCGATCC TCACACTGGG ACGAGTATTC TTTATGTACC TGCTGTCTAT
    GGAGGGAATG TCGTTATGTC GGTGCCTGTA CCTGTGCCAT GGACAGGATA CCAGGGCAGG
    TTTGCGGTTG ATCCTCGAAT CATTACACAC CAGGCAGCAA TGGCCTACAA CATGAACCTC
    TTACAGACAC ATGGACGTGG GTCTCCTATT CCTTATGGCC TTGGGCACCA CCCACCTGTC
    AGCATAGGGC AGCCACAGAA TCAGCATCAG GAGAAGGATC AACATGAGCA AAGTCGCAAC
    GGTAAAAGTG ATACAAATAA TCCTGGACCT GAAATTAATA AGATTCGAAC ACCAGAGAAA
    AAACCTACAG AAGCAAAACA GATTGATTTG GAATCAAACC CACAGAACAG AAGTCCTGAA
    TCACGTTCTG GCATTGTTTA TCCCCATCCC AAATTTCATC GCAAAGATAA TCTCACCCCA
    AGACACATAA ACCTTCCTCT CCCTGCCCCC CATGCCCAGT ATGCCATCCC CGGTCGTCAT
    TTTCATCCCC TCCCCCAGCT CCCGAGACCA CCCTTCCCAA TTCCGCAGCA GCACGCCTTG
    CTGAATCAGC AGCAGAGTAA CTTGCCTGAA CAGCCGAGCC CCGTGCCAGC CCAGCCCAGT
    CAGGTGGCCC CTCAGCTGGG CCCGCCGCCG CCTCAGCTCT CCCCTGCCTA CCCGGCTGGC
    CCCGGCAGCG CGTTTTTCAA TAACGCGGTT CCCCACCGAC CACAGTCCCC GCCTGCAGAG
    GCTGTGGTTC CCGAGCAGCA GCCCCCACCC GTGCTGCAGG AGGGCCACAG TCCCCTGCGG
    GCCCTCGCAC AGCCCGGCCC CATCCTGCCT TCACCTCTGA ACAGCTTCCC GGACGAGAGC
    CCCCCGGGCC TGCCGATGGG GGAGGCTTTG GATCGTATGC ACGGGAGTGT GGCTCTGGAA
    ACCCTGAGGC AGCAGCATGT GCGGCTACAG CAGTGGAGCG AGCACCACGC CTATCTCAGT
    CAGGGCAGCC TCCCGTACCC GCACCATCAC CACCCTCACC CACACCTCCA GCACCTTCCC
    CAGCCGCCCG TTGGATTGCA TCAGCCCCCA GTGAGAGCAG ACTGGAAACT CCCCAGCAGT
    GCCGAAGATG AAGCAGAAAC AACTGACTCA CGGTTTCAAG ACTTACTCAG AGAACTGTCT
    AATCGTGAGC AAAGTGAAAC ACGGGAACTA GCTGAAATGC CACCACCTCA ATCAAGGCTT
    TTGCAATATA GACAAATTCA GACTAGGAGC CCACCAGCAG CCCCCTCGCC CCCGTCCGGC
    GCAGACCACA GCACCCACTT TCCAAACTTC ACTGACAGCA GCAGAGACCT TGAAGTGGCC
    AACAGCCCGG CGTTCCCACA GCGCCTGCCG CCCCCGATGT TTAGCTCTCC TTTCTCGTTG
    CCATCTGAGC ACCTCACCCC TCCTCTGAAA TACCTGGCGC CTGATGGAGC TTGGACTTTT
    GCTAACTTAC AGCAGAATCA CCTGATGGGG CCGAGTTTCC CCTACGGCCT ACCTCCATTG
    CCTCACAGGC CCCCGCAGAA CCCTTTCGTG CAAATGCAGA CCCACCAGCC CGCTGTTGGC
    CAGGAGCCGC TCCAGCCGCT GTCCTCTCGG ACAGTGTCGT CCTCTTCGCT CCCCAGCTTA
    GAAGAGTATG AACCCAGAGG ACCGGGTCGG CCCTTGTACC AAAGAAGAAT CTCATCTAGC
    TCAGTGCAGC CTTGTTCTGA AGAAGCAAGC ACTCCTCAAG ACAGTCTTGC TCAGCGTAAA
    GAGCTTCAGG ACCATAATAA CCAGTCTTCT TTCACCTTTT CCTCCCCGGA GTCCTGGGTA
    AACGCCACCT CATCCACCCC CTATCAGAAC ATTCCGTGCA ATGGATCCAG CAGGCCAGCC
    CAGCCCAGAG AGTTGGCAGC TCCACCCAAG ACCGTCAAAG CCCCCGAGGA ACATCTGAAG
    CCTGAGAGCC TTGAGGGGTC CAGTTCCTTC AATTACAGCG TGCTGCAGCA TCTCGGCCAG
    TTCCCCCCGC TCATGCCCAA CAAGCAGATC GCCGAGTCGG CCAGCAGCAG CAGCCCGCAG
    AGCTCCGGGG GCGGCAAGCC TGCCATGTCC TACGCCAGCG CCCTGCGGGC CCCTCCAAAG
    CCCAGGCCCC CGCCCGAGCA GGCCAAGAAG AGCAGCGACC CTCTGTCTCT GTTTCAAGAA
    CTCAGCCTGG GGAGCTCAGC CGGCAGCAAT GGCTTTTACT CCTATTTTAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836901.1
    CDS208..6021
    Translation

    Target ORF information:

    RefSeq Version XM_024981133.1
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase with zinc finger (HELZ), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981133.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGCAGGA TTCTCATTTG CACCCATTCT
    AATAGTGCTG CTGATCTCTA CATAAAGGAT TATTTACATC CATATGTAGA AGCAGGCAAT
    CCCCAGGCAA GACCTCTCAG GGTATATTTC AGAAACCGCT GGGTGAAGAC TGTCCACCCA
    GTTGTGCATC AGTACTGTTT GATCTCAAGC GCACATTCCA CCTTTCAGAT GCCACAGAAA
    GAAGATATTC TGAAACACCG AGTGGTGGTG GTTACATTGA ACACTTCCCA GTACCTCTGT
    CAGCTGGACC TCGAACCTGG GTTTTTTACT CACATCTTGT TAGACGAGGC TGCCCAGGCC
    ATGGAGTGTG AAACCATCAT GCCTCTGGCA CTAGCAACTA GACACACGCG GGTTGTCCTG
    GCTGGCGACC ACATGCAGCT CAGTCCTTTT GTTTACAGCG AGTTTGCCAG GGAGAGGAAC
    CTTCACGTGT CGTTACTTGA TCGGCTCTAT GAGCACTACC CTGCCGAGTT TCCCTGTAGG
    ATCCTCCTGT GCGAGAACTA CCGTTCCCAC GAAGCTATCA TCAATTACAC CTCTGAGCTC
    TTCTATGAGG GCAAGCTGAT GGCCAGTGGA AAGCAACCAG CACACAAGGA GTTCTATCCA
    CTAACCTTCT TTACAGCACG AGGGGAAGAT GTACAGGAGA AAAATAGCAC AGCTTTTTAT
    AATAATGCAG AGGTATTTGA AGTGGTGGAG CGAGTGGAAG AGCTGAGAAG GAAGTGGCCA
    GTAGCGTGGG GCAAGTTGGA TGATGGAAGT ATTGGTGTGG TGACTCCATA TGCAGACCAG
    GTGTTCAGGA TACGCGCCGA ACTTCGAAAA AAGAGGTTAT CTGACGTTAA CGTAGAAAGG
    GTGCTCAATG TTCAAGGAAA GCAATTCAGA GTTTTGTTTC TTAGCACAGT ACGTACAAGG
    CATACTTGTA AACATAAACA GACACCAATT AAAAAGAAGG AGCAGCTGCT AGAAGACTCC
    ACAGAGGACT TGGATTATGG CTTTTTGTCT AACTACAAGC TCCTCAACAC CGCCATCACA
    AGAGCACAGT CCCTGGTTGC CGTGGTGGGG GACCCCATCG CTCTGTGCTC CATTGGGAGA
    TGCAGGAAAT TTTGGGAGCG GTTTATTGCC CTGTGTCATG AAAACAATAG CCTGCATGGA
    ATCACTTTTG AACAGATCAA AGCGCAGCTA GAGGCTTTAG AACTAAAGAA GACATACGTG
    TTGAATCCAC TGGCACCTGA ATTTATACCC CGGGCTCTAA GAGCACAGCA CGCGGGAAAC
    ACCAACAAAC CGCAGCAATC ACCCCCCAAG GGAAAAAGCC TCCATCATAC CCAGAATGAT
    CATTTCCAGA ATGATGGAAT TGTTCAGCCC AATCCCTCTG TGCTTATTGG CAATCCTATC
    AGAGCATATA CTCCTCCACC TCCTCTTGGA CCTCACCCAA ATTTGGGGAA ATCTCCAAGT
    CCTGTTCAAA GAATCGATCC TCACACTGGG ACGAGTATTC TTTATGTACC TGCTGTCTAT
    GGAGGGAATG TCGTTATGTC GGTGCCTGTA CCTGTGCCAT GGACAGGATA CCAGGGCAGG
    TTTGCGGTTG ATCCTCGAAT CATTACACAC CAGGCAGCAA TGGCCTACAA CATGAACCTC
    TTACAGACAC ATGGACGTGG GTCTCCTATT CCTTATGGCC TTGGGCACCA CCCACCTGTC
    AGCATAGGGC AGCCACAGAA TCAGCATCAG GAGAAGGATC AACATGAGCA AAGTCGCAAC
    GGTAAAAGTG ATACAAATAA TCCTGGACCT GAAATTAATA AGATTCGAAC ACCAGAGAAA
    AAACCTACAG AAGCAAAACA GATTGATTTG GAATCAAACC CACAGAACAG AAGTCCTGAA
    TCACGTTCTG GCATTGTTTA TCCCCATCCC AAATTTCATC GCAAAGATAA TCTCACCCCA
    AGACACATAA ACCTTCCTCT CCCTGCCCCC CATGCCCAGT ATGCCATCCC CGGTCGTCAT
    TTTCATCCCC TCCCCCAGCT CCCGAGACCA CCCTTCCCAA TTCCGCAGCA GCACGCCTTG
    CTGAATCAGC AGCAGAGTAA CTTGCCTGAA CAGCCGAGCC CCGTGCCAGC CCAGCCCAGT
    CAGGTGGCCC CTCAGCTGGG CCCGCCGCCG CCTCAGCTCT CCCCTGCCTA CCCGGCTGGC
    CCCGGCAGCG CGTTTTTCAA TAACGCGGTT CCCCACCGAC CACAGTCCCC GCCTGCAGAG
    GCTGTGGTTC CCGAGCAGCA GCCCCCACCC GTGCTGCAGG AGGGCCACAG TCCCCTGCGG
    GCCCTCGCAC AGCCCGGCCC CATCCTGCCT TCACCTCTGA ACAGCTTCCC GGACGAGAGC
    CCCCCGGGCC TGCCGATGGG GGAGGCTTTG GATCGTATGC ACGGGAGTGT GGCTCTGGAA
    ACCCTGAGGC AGCAGCATGT GCGGCTACAG CAGTGGAGCG AGCACCACGC CTATCTCAGT
    CAGGGCAGCC TCCCGTACCC GCACCATCAC CACCCTCACC CACACCTCCA GCACCTTCCC
    CAGCCGCCCG TTGGATTGCA TCAGCCCCCA GTGAGAGCAG ACTGGAAACT CCCCAGCAGT
    GCCGAAGATG AAGCAGAAAC AACTGACTCA CGGTTTCAAG ACTTACTCAG AGAACTGTCT
    AATCGTGAGC AAAGTGAAAC ACGGGAACTA GCTGAAATGC CACCACCTCA ATCAAGGCTT
    TTGCAATATA GACAAATTCA GACTAGGAGC CCACCAGCAG CCCCCTCGCC CCCGTCCGGC
    GCAGACCACA GCACCCACTT TCCAAACTTC ACTGACAGCA GCAGAGACCT TGAAGTGGCC
    AACAGCCCGG CGTTCCCACA GCGCCTGCCG CCCCCGATGT TTAGCTCTCC TTTCTCGTTG
    CCATCTGAGC ACCTCACCCC TCCTCTGAAA TACCTGGCGC CTGATGGAGC TTGGACTTTT
    GCTAACTTAC AGCAGAATCA CCTGATGGGG CCGAGTTTCC CCTACGGCCT ACCTCCATTG
    CCTCACAGGC CCCCGCAGAA CCCTTTCGTG CAAATGCAGA CCCACCAGCC CGCTGTTGGC
    CAGGAGCCGC TCCAGCCGCT GTCCTCTCGG ACAGTGTCGT CCTCTTCGCT CCCCAGCTTA
    GAAGAGTATG AACCCAGAGG ACCGGGTCGG CCCTTGTACC AAAGAAGAAT CTCATCTAGC
    TCAGTGCAGC CTTGTTCTGA AGAAGCAAGC ACTCCTCAAG ACAGTCTTGC TCAGCGTAAA
    GAGCTTCAGG ACCATAATAA CCAGTCTTCT TTCACCTTTT CCTCCCCGGA GTCCTGGGTA
    AACGCCACCT CATCCACCCC CTATCAGAAC ATTCCGTGCA ATGGATCCAG CAGGCCAGCC
    CAGCCCAGAG AGTTGGCAGC TCCACCCAAG ACCGTCAAAG CCCCCGAGGA ACATCTGAAG
    CCTGAGAGCC TTGAGGGGTC CAGTTCCTTC AATTACAGCG TGCTGCAGCA TCTCGGCCAG
    TTCCCCCCGC TCATGCCCAA CAAGCAGATC GCCGAGTCGG CCAGCAGCAG CAGCCCGCAG
    AGCTCCGGGG GCGGCAAGCC TGCCATGTCC TACGCCAGCG CCCTGCGGGC CCCTCCAAAG
    CCCAGGCCCC CGCCCGAGCA GGCCAAGAAG AGCAGCGACC CTCTGTCTCT GTTTCAAGAA
    CTCAGCCTGG GGAGCTCAGC CGGCAGCAAT GGCTTTTACT CCTATTTTAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227074
    Clone ID Related Accession (Same CDS sequence) XM_024981135.1 , XM_024981134.1 , XM_024981131.1 , XM_024981133.1 , XM_024981132.1
    Accession Version XM_024981132.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable helicase with zinc finger domain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGCAGGA TTCTCATTTG CACCCATTCT
    AATAGTGCTG CTGATCTCTA CATAAAGGAT TATTTACATC CATATGTAGA AGCAGGCAAT
    CCCCAGGCAA GACCTCTCAG GGTATATTTC AGAAACCGCT GGGTGAAGAC TGTCCACCCA
    GTTGTGCATC AGTACTGTTT GATCTCAAGC GCACATTCCA CCTTTCAGAT GCCACAGAAA
    GAAGATATTC TGAAACACCG AGTGGTGGTG GTTACATTGA ACACTTCCCA GTACCTCTGT
    CAGCTGGACC TCGAACCTGG GTTTTTTACT CACATCTTGT TAGACGAGGC TGCCCAGGCC
    ATGGAGTGTG AAACCATCAT GCCTCTGGCA CTAGCAACTA GACACACGCG GGTTGTCCTG
    GCTGGCGACC ACATGCAGCT CAGTCCTTTT GTTTACAGCG AGTTTGCCAG GGAGAGGAAC
    CTTCACGTGT CGTTACTTGA TCGGCTCTAT GAGCACTACC CTGCCGAGTT TCCCTGTAGG
    ATCCTCCTGT GCGAGAACTA CCGTTCCCAC GAAGCTATCA TCAATTACAC CTCTGAGCTC
    TTCTATGAGG GCAAGCTGAT GGCCAGTGGA AAGCAACCAG CACACAAGGA GTTCTATCCA
    CTAACCTTCT TTACAGCACG AGGGGAAGAT GTACAGGAGA AAAATAGCAC AGCTTTTTAT
    AATAATGCAG AGGTATTTGA AGTGGTGGAG CGAGTGGAAG AGCTGAGAAG GAAGTGGCCA
    GTAGCGTGGG GCAAGTTGGA TGATGGAAGT ATTGGTGTGG TGACTCCATA TGCAGACCAG
    GTGTTCAGGA TACGCGCCGA ACTTCGAAAA AAGAGGTTAT CTGACGTTAA CGTAGAAAGG
    GTGCTCAATG TTCAAGGAAA GCAATTCAGA GTTTTGTTTC TTAGCACAGT ACGTACAAGG
    CATACTTGTA AACATAAACA GACACCAATT AAAAAGAAGG AGCAGCTGCT AGAAGACTCC
    ACAGAGGACT TGGATTATGG CTTTTTGTCT AACTACAAGC TCCTCAACAC CGCCATCACA
    AGAGCACAGT CCCTGGTTGC CGTGGTGGGG GACCCCATCG CTCTGTGCTC CATTGGGAGA
    TGCAGGAAAT TTTGGGAGCG GTTTATTGCC CTGTGTCATG AAAACAATAG CCTGCATGGA
    ATCACTTTTG AACAGATCAA AGCGCAGCTA GAGGCTTTAG AACTAAAGAA GACATACGTG
    TTGAATCCAC TGGCACCTGA ATTTATACCC CGGGCTCTAA GAGCACAGCA CGCGGGAAAC
    ACCAACAAAC CGCAGCAATC ACCCCCCAAG GGAAAAAGCC TCCATCATAC CCAGAATGAT
    CATTTCCAGA ATGATGGAAT TGTTCAGCCC AATCCCTCTG TGCTTATTGG CAATCCTATC
    AGAGCATATA CTCCTCCACC TCCTCTTGGA CCTCACCCAA ATTTGGGGAA ATCTCCAAGT
    CCTGTTCAAA GAATCGATCC TCACACTGGG ACGAGTATTC TTTATGTACC TGCTGTCTAT
    GGAGGGAATG TCGTTATGTC GGTGCCTGTA CCTGTGCCAT GGACAGGATA CCAGGGCAGG
    TTTGCGGTTG ATCCTCGAAT CATTACACAC CAGGCAGCAA TGGCCTACAA CATGAACCTC
    TTACAGACAC ATGGACGTGG GTCTCCTATT CCTTATGGCC TTGGGCACCA CCCACCTGTC
    AGCATAGGGC AGCCACAGAA TCAGCATCAG GAGAAGGATC AACATGAGCA AAGTCGCAAC
    GGTAAAAGTG ATACAAATAA TCCTGGACCT GAAATTAATA AGATTCGAAC ACCAGAGAAA
    AAACCTACAG AAGCAAAACA GATTGATTTG GAATCAAACC CACAGAACAG AAGTCCTGAA
    TCACGTTCTG GCATTGTTTA TCCCCATCCC AAATTTCATC GCAAAGATAA TCTCACCCCA
    AGACACATAA ACCTTCCTCT CCCTGCCCCC CATGCCCAGT ATGCCATCCC CGGTCGTCAT
    TTTCATCCCC TCCCCCAGCT CCCGAGACCA CCCTTCCCAA TTCCGCAGCA GCACGCCTTG
    CTGAATCAGC AGCAGAGTAA CTTGCCTGAA CAGCCGAGCC CCGTGCCAGC CCAGCCCAGT
    CAGGTGGCCC CTCAGCTGGG CCCGCCGCCG CCTCAGCTCT CCCCTGCCTA CCCGGCTGGC
    CCCGGCAGCG CGTTTTTCAA TAACGCGGTT CCCCACCGAC CACAGTCCCC GCCTGCAGAG
    GCTGTGGTTC CCGAGCAGCA GCCCCCACCC GTGCTGCAGG AGGGCCACAG TCCCCTGCGG
    GCCCTCGCAC AGCCCGGCCC CATCCTGCCT TCACCTCTGA ACAGCTTCCC GGACGAGAGC
    CCCCCGGGCC TGCCGATGGG GGAGGCTTTG GATCGTATGC ACGGGAGTGT GGCTCTGGAA
    ACCCTGAGGC AGCAGCATGT GCGGCTACAG CAGTGGAGCG AGCACCACGC CTATCTCAGT
    CAGGGCAGCC TCCCGTACCC GCACCATCAC CACCCTCACC CACACCTCCA GCACCTTCCC
    CAGCCGCCCG TTGGATTGCA TCAGCCCCCA GTGAGAGCAG ACTGGAAACT CCCCAGCAGT
    GCCGAAGATG AAGCAGAAAC AACTGACTCA CGGTTTCAAG ACTTACTCAG AGAACTGTCT
    AATCGTGAGC AAAGTGAAAC ACGGGAACTA GCTGAAATGC CACCACCTCA ATCAAGGCTT
    TTGCAATATA GACAAATTCA GACTAGGAGC CCACCAGCAG CCCCCTCGCC CCCGTCCGGC
    GCAGACCACA GCACCCACTT TCCAAACTTC ACTGACAGCA GCAGAGACCT TGAAGTGGCC
    AACAGCCCGG CGTTCCCACA GCGCCTGCCG CCCCCGATGT TTAGCTCTCC TTTCTCGTTG
    CCATCTGAGC ACCTCACCCC TCCTCTGAAA TACCTGGCGC CTGATGGAGC TTGGACTTTT
    GCTAACTTAC AGCAGAATCA CCTGATGGGG CCGAGTTTCC CCTACGGCCT ACCTCCATTG
    CCTCACAGGC CCCCGCAGAA CCCTTTCGTG CAAATGCAGA CCCACCAGCC CGCTGTTGGC
    CAGGAGCCGC TCCAGCCGCT GTCCTCTCGG ACAGTGTCGT CCTCTTCGCT CCCCAGCTTA
    GAAGAGTATG AACCCAGAGG ACCGGGTCGG CCCTTGTACC AAAGAAGAAT CTCATCTAGC
    TCAGTGCAGC CTTGTTCTGA AGAAGCAAGC ACTCCTCAAG ACAGTCTTGC TCAGCGTAAA
    GAGCTTCAGG ACCATAATAA CCAGTCTTCT TTCACCTTTT CCTCCCCGGA GTCCTGGGTA
    AACGCCACCT CATCCACCCC CTATCAGAAC ATTCCGTGCA ATGGATCCAG CAGGCCAGCC
    CAGCCCAGAG AGTTGGCAGC TCCACCCAAG ACCGTCAAAG CCCCCGAGGA ACATCTGAAG
    CCTGAGAGCC TTGAGGGGTC CAGTTCCTTC AATTACAGCG TGCTGCAGCA TCTCGGCCAG
    TTCCCCCCGC TCATGCCCAA CAAGCAGATC GCCGAGTCGG CCAGCAGCAG CAGCCCGCAG
    AGCTCCGGGG GCGGCAAGCC TGCCATGTCC TACGCCAGCG CCCTGCGGGC CCCTCCAAAG
    CCCAGGCCCC CGCCCGAGCA GGCCAAGAAG AGCAGCGACC CTCTGTCTCT GTTTCAAGAA
    CTCAGCCTGG GGAGCTCAGC CGGCAGCAAT GGCTTTTACT CCTATTTTAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836900.1
    CDS313..6126
    Translation

    Target ORF information:

    RefSeq Version XM_024981132.1
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase with zinc finger (HELZ), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981132.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGCAGGA TTCTCATTTG CACCCATTCT
    AATAGTGCTG CTGATCTCTA CATAAAGGAT TATTTACATC CATATGTAGA AGCAGGCAAT
    CCCCAGGCAA GACCTCTCAG GGTATATTTC AGAAACCGCT GGGTGAAGAC TGTCCACCCA
    GTTGTGCATC AGTACTGTTT GATCTCAAGC GCACATTCCA CCTTTCAGAT GCCACAGAAA
    GAAGATATTC TGAAACACCG AGTGGTGGTG GTTACATTGA ACACTTCCCA GTACCTCTGT
    CAGCTGGACC TCGAACCTGG GTTTTTTACT CACATCTTGT TAGACGAGGC TGCCCAGGCC
    ATGGAGTGTG AAACCATCAT GCCTCTGGCA CTAGCAACTA GACACACGCG GGTTGTCCTG
    GCTGGCGACC ACATGCAGCT CAGTCCTTTT GTTTACAGCG AGTTTGCCAG GGAGAGGAAC
    CTTCACGTGT CGTTACTTGA TCGGCTCTAT GAGCACTACC CTGCCGAGTT TCCCTGTAGG
    ATCCTCCTGT GCGAGAACTA CCGTTCCCAC GAAGCTATCA TCAATTACAC CTCTGAGCTC
    TTCTATGAGG GCAAGCTGAT GGCCAGTGGA AAGCAACCAG CACACAAGGA GTTCTATCCA
    CTAACCTTCT TTACAGCACG AGGGGAAGAT GTACAGGAGA AAAATAGCAC AGCTTTTTAT
    AATAATGCAG AGGTATTTGA AGTGGTGGAG CGAGTGGAAG AGCTGAGAAG GAAGTGGCCA
    GTAGCGTGGG GCAAGTTGGA TGATGGAAGT ATTGGTGTGG TGACTCCATA TGCAGACCAG
    GTGTTCAGGA TACGCGCCGA ACTTCGAAAA AAGAGGTTAT CTGACGTTAA CGTAGAAAGG
    GTGCTCAATG TTCAAGGAAA GCAATTCAGA GTTTTGTTTC TTAGCACAGT ACGTACAAGG
    CATACTTGTA AACATAAACA GACACCAATT AAAAAGAAGG AGCAGCTGCT AGAAGACTCC
    ACAGAGGACT TGGATTATGG CTTTTTGTCT AACTACAAGC TCCTCAACAC CGCCATCACA
    AGAGCACAGT CCCTGGTTGC CGTGGTGGGG GACCCCATCG CTCTGTGCTC CATTGGGAGA
    TGCAGGAAAT TTTGGGAGCG GTTTATTGCC CTGTGTCATG AAAACAATAG CCTGCATGGA
    ATCACTTTTG AACAGATCAA AGCGCAGCTA GAGGCTTTAG AACTAAAGAA GACATACGTG
    TTGAATCCAC TGGCACCTGA ATTTATACCC CGGGCTCTAA GAGCACAGCA CGCGGGAAAC
    ACCAACAAAC CGCAGCAATC ACCCCCCAAG GGAAAAAGCC TCCATCATAC CCAGAATGAT
    CATTTCCAGA ATGATGGAAT TGTTCAGCCC AATCCCTCTG TGCTTATTGG CAATCCTATC
    AGAGCATATA CTCCTCCACC TCCTCTTGGA CCTCACCCAA ATTTGGGGAA ATCTCCAAGT
    CCTGTTCAAA GAATCGATCC TCACACTGGG ACGAGTATTC TTTATGTACC TGCTGTCTAT
    GGAGGGAATG TCGTTATGTC GGTGCCTGTA CCTGTGCCAT GGACAGGATA CCAGGGCAGG
    TTTGCGGTTG ATCCTCGAAT CATTACACAC CAGGCAGCAA TGGCCTACAA CATGAACCTC
    TTACAGACAC ATGGACGTGG GTCTCCTATT CCTTATGGCC TTGGGCACCA CCCACCTGTC
    AGCATAGGGC AGCCACAGAA TCAGCATCAG GAGAAGGATC AACATGAGCA AAGTCGCAAC
    GGTAAAAGTG ATACAAATAA TCCTGGACCT GAAATTAATA AGATTCGAAC ACCAGAGAAA
    AAACCTACAG AAGCAAAACA GATTGATTTG GAATCAAACC CACAGAACAG AAGTCCTGAA
    TCACGTTCTG GCATTGTTTA TCCCCATCCC AAATTTCATC GCAAAGATAA TCTCACCCCA
    AGACACATAA ACCTTCCTCT CCCTGCCCCC CATGCCCAGT ATGCCATCCC CGGTCGTCAT
    TTTCATCCCC TCCCCCAGCT CCCGAGACCA CCCTTCCCAA TTCCGCAGCA GCACGCCTTG
    CTGAATCAGC AGCAGAGTAA CTTGCCTGAA CAGCCGAGCC CCGTGCCAGC CCAGCCCAGT
    CAGGTGGCCC CTCAGCTGGG CCCGCCGCCG CCTCAGCTCT CCCCTGCCTA CCCGGCTGGC
    CCCGGCAGCG CGTTTTTCAA TAACGCGGTT CCCCACCGAC CACAGTCCCC GCCTGCAGAG
    GCTGTGGTTC CCGAGCAGCA GCCCCCACCC GTGCTGCAGG AGGGCCACAG TCCCCTGCGG
    GCCCTCGCAC AGCCCGGCCC CATCCTGCCT TCACCTCTGA ACAGCTTCCC GGACGAGAGC
    CCCCCGGGCC TGCCGATGGG GGAGGCTTTG GATCGTATGC ACGGGAGTGT GGCTCTGGAA
    ACCCTGAGGC AGCAGCATGT GCGGCTACAG CAGTGGAGCG AGCACCACGC CTATCTCAGT
    CAGGGCAGCC TCCCGTACCC GCACCATCAC CACCCTCACC CACACCTCCA GCACCTTCCC
    CAGCCGCCCG TTGGATTGCA TCAGCCCCCA GTGAGAGCAG ACTGGAAACT CCCCAGCAGT
    GCCGAAGATG AAGCAGAAAC AACTGACTCA CGGTTTCAAG ACTTACTCAG AGAACTGTCT
    AATCGTGAGC AAAGTGAAAC ACGGGAACTA GCTGAAATGC CACCACCTCA ATCAAGGCTT
    TTGCAATATA GACAAATTCA GACTAGGAGC CCACCAGCAG CCCCCTCGCC CCCGTCCGGC
    GCAGACCACA GCACCCACTT TCCAAACTTC ACTGACAGCA GCAGAGACCT TGAAGTGGCC
    AACAGCCCGG CGTTCCCACA GCGCCTGCCG CCCCCGATGT TTAGCTCTCC TTTCTCGTTG
    CCATCTGAGC ACCTCACCCC TCCTCTGAAA TACCTGGCGC CTGATGGAGC TTGGACTTTT
    GCTAACTTAC AGCAGAATCA CCTGATGGGG CCGAGTTTCC CCTACGGCCT ACCTCCATTG
    CCTCACAGGC CCCCGCAGAA CCCTTTCGTG CAAATGCAGA CCCACCAGCC CGCTGTTGGC
    CAGGAGCCGC TCCAGCCGCT GTCCTCTCGG ACAGTGTCGT CCTCTTCGCT CCCCAGCTTA
    GAAGAGTATG AACCCAGAGG ACCGGGTCGG CCCTTGTACC AAAGAAGAAT CTCATCTAGC
    TCAGTGCAGC CTTGTTCTGA AGAAGCAAGC ACTCCTCAAG ACAGTCTTGC TCAGCGTAAA
    GAGCTTCAGG ACCATAATAA CCAGTCTTCT TTCACCTTTT CCTCCCCGGA GTCCTGGGTA
    AACGCCACCT CATCCACCCC CTATCAGAAC ATTCCGTGCA ATGGATCCAG CAGGCCAGCC
    CAGCCCAGAG AGTTGGCAGC TCCACCCAAG ACCGTCAAAG CCCCCGAGGA ACATCTGAAG
    CCTGAGAGCC TTGAGGGGTC CAGTTCCTTC AATTACAGCG TGCTGCAGCA TCTCGGCCAG
    TTCCCCCCGC TCATGCCCAA CAAGCAGATC GCCGAGTCGG CCAGCAGCAG CAGCCCGCAG
    AGCTCCGGGG GCGGCAAGCC TGCCATGTCC TACGCCAGCG CCCTGCGGGC CCCTCCAAAG
    CCCAGGCCCC CGCCCGAGCA GGCCAAGAAG AGCAGCGACC CTCTGTCTCT GTTTCAAGAA
    CTCAGCCTGG GGAGCTCAGC CGGCAGCAAT GGCTTTTACT CCTATTTTAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227075
    Clone ID Related Accession (Same CDS sequence) XM_024981137.1 , XM_024981136.1 , XM_024981139.1
    Accession Version XM_024981137.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5811bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable helicase with zinc finger domain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGGATTC TCATTTGCAC CCATTCTAAT
    AGTGCTGCTG ATCTCTACAT AAAGGATTAT TTACATCCAT ATGTAGAAGC AGGCAATCCC
    CAGGCAAGAC CTCTCAGGGT ATATTTCAGA AACCGCTGGG TGAAGACTGT CCACCCAGTT
    GTGCATCAGT ACTGTTTGAT CTCAAGCGCA CATTCCACCT TTCAGATGCC ACAGAAAGAA
    GATATTCTGA AACACCGAGT GGTGGTGGTT ACATTGAACA CTTCCCAGTA CCTCTGTCAG
    CTGGACCTCG AACCTGGGTT TTTTACTCAC ATCTTGTTAG ACGAGGCTGC CCAGGCCATG
    GAGTGTGAAA CCATCATGCC TCTGGCACTA GCAACTAGAC ACACGCGGGT TGTCCTGGCT
    GGCGACCACA TGCAGCTCAG TCCTTTTGTT TACAGCGAGT TTGCCAGGGA GAGGAACCTT
    CACGTGTCGT TACTTGATCG GCTCTATGAG CACTACCCTG CCGAGTTTCC CTGTAGGATC
    CTCCTGTGCG AGAACTACCG TTCCCACGAA GCTATCATCA ATTACACCTC TGAGCTCTTC
    TATGAGGGCA AGCTGATGGC CAGTGGAAAG CAACCAGCAC ACAAGGAGTT CTATCCACTA
    ACCTTCTTTA CAGCACGAGG GGAAGATGTA CAGGAGAAAA ATAGCACAGC TTTTTATAAT
    AATGCAGAGG TATTTGAAGT GGTGGAGCGA GTGGAAGAGC TGAGAAGGAA GTGGCCAGTA
    GCGTGGGGCA AGTTGGATGA TGGAAGTATT GGTGTGGTGA CTCCATATGC AGACCAGGTG
    TTCAGGATAC GCGCCGAACT TCGAAAAAAG AGGTTATCTG ACGTTAACGT AGAAAGGGTG
    CTCAATGTTC AAGGAAAGCA ATTCAGAGTT TTGTTTCTTA GCACAGTACG TACAAGGCAT
    ACTTGTAAAC ATAAACAGAC ACCAATTAAA AAGAAGGAGC AGCTGCTAGA AGACTCCACA
    GAGGACTTGG ATTATGGCTT TTTGTCTAAC TACAAGCTCC TCAACACCGC CATCACAAGA
    GCACAGTCCC TGGTTGCCGT GGTGGGGGAC CCCATCGCTC TGTGCTCCAT TGGGAGATGC
    AGGAAATTTT GGGAGCGGTT TATTGCCCTG TGTCATGAAA ACAATAGCCT GCATGGAATC
    ACTTTTGAAC AGATCAAAGC GCAGCTAGAG GCTTTAGAAC TAAAGAAGAC ATACGTGTTG
    AATCCACTGG CACCTGAATT TATACCCCGG GCTCTAAGAG CACAGCACGC GGGAAACACC
    AACAAACCGC AGCAATCACC CCCCAAGGGA AAAAGCCTCC ATCATACCCA GAATGATCAT
    TTCCAGAATG ATGGAATTGT TCAGCCCAAT CCCTCTGTGC TTATTGGCAA TCCTATCAGA
    GCATATACTC CTCCACCTCC TCTTGGACCT CACCCAAATT TGGGGAAATC TCCAAGTCCT
    GTTCAAAGAA TCGATCCTCA CACTGGGACG AGTATTCTTT ATGTACCTGC TGTCTATGGA
    GGGAATGTCG TTATGTCGGT GCCTGTACCT GTGCCATGGA CAGGATACCA GGGCAGGTTT
    GCGGTTGATC CTCGAATCAT TACACACCAG GCAGCAATGG CCTACAACAT GAACCTCTTA
    CAGACACATG GACGTGGGTC TCCTATTCCT TATGGCCTTG GGCACCACCC ACCTGTCAGC
    ATAGGGCAGC CACAGAATCA GCATCAGGAG AAGGATCAAC ATGAGCAAAG TCGCAACGGT
    AAAAGTGATA CAAATAATCC TGGACCTGAA ATTAATAAGA TTCGAACACC AGAGAAAAAA
    CCTACAGAAG CAAAACAGAT TGATTTGGAA TCAAACCCAC AGAACAGAAG TCCTGAATCA
    CGTTCTGGCA TTGTTTATCC CCATCCCAAA TTTCATCGCA AAGATAATCT CACCCCAAGA
    CACATAAACC TTCCTCTCCC TGCCCCCCAT GCCCAGTATG CCATCCCCGG TCGTCATTTT
    CATCCCCTCC CCCAGCTCCC GAGACCACCC TTCCCAATTC CGCAGCAGCA CGCCTTGCTG
    AATCAGCAGC AGAGTAACTT GCCTGAACAG CCGAGCCCCG TGCCAGCCCA GCCCAGTCAG
    GTGGCCCCTC AGCTGGGCCC GCCGCCGCCT CAGCTCTCCC CTGCCTACCC GGCTGGCCCC
    GGCAGCGCGT TTTTCAATAA CGCGGTTCCC CACCGACCAC AGTCCCCGCC TGCAGAGGCT
    GTGGTTCCCG AGCAGCAGCC CCCACCCGTG CTGCAGGAGG GCCACAGTCC CCTGCGGGCC
    CTCGCACAGC CCGGCCCCAT CCTGCCTTCA CCTCTGAACA GCTTCCCGGA CGAGAGCCCC
    CCGGGCCTGC CGATGGGGGA GGCTTTGGAT CGTATGCACG GGAGTGTGGC TCTGGAAACC
    CTGAGGCAGC AGCATGTGCG GCTACAGCAG TGGAGCGAGC ACCACGCCTA TCTCAGTCAG
    GGCAGCCTCC CGTACCCGCA CCATCACCAC CCTCACCCAC ACCTCCAGCA CCTTCCCCAG
    CCGCCCGTTG GATTGCATCA GCCCCCAGTG AGAGCAGACT GGAAACTCCC CAGCAGTGCC
    GAAGATGAAG CAGAAACAAC TGACTCACGG TTTCAAGACT TACTCAGAGA ACTGTCTAAT
    CGTGAGCAAA GTGAAACACG GGAACTAGCT GAAATGCCAC CACCTCAATC AAGGCTTTTG
    CAATATAGAC AAATTCAGAC TAGGAGCCCA CCAGCAGCCC CCTCGCCCCC GTCCGGCGCA
    GACCACAGCA CCCACTTTCC AAACTTCACT GACAGCAGCA GAGACCTTGA AGTGGCCAAC
    AGCCCGGCGT TCCCACAGCG CCTGCCGCCC CCGATGTTTA GCTCTCCTTT CTCGTTGCCA
    TCTGAGCACC TCACCCCTCC TCTGAAATAC CTGGCGCCTG ATGGAGCTTG GACTTTTGCT
    AACTTACAGC AGAATCACCT GATGGGGCCG AGTTTCCCCT ACGGCCTACC TCCATTGCCT
    CACAGGCCCC CGCAGAACCC TTTCGTGCAA ATGCAGACCC ACCAGCCCGC TGTTGGCCAG
    GAGCCGCTCC AGCCGCTGTC CTCTCGGACA GTGTCGTCCT CTTCGCTCCC CAGCTTAGAA
    GAGTATGAAC CCAGAGGACC GGGTCGGCCC TTGTACCAAA GAAGAATCTC ATCTAGCTCA
    GTGCAGCCTT GTTCTGAAGA AGCAAGCACT CCTCAAGACA GTCTTGCTCA GCGTAAAGAG
    CTTCAGGACC ATAATAACCA GTCTTCTTTC ACCTTTTCCT CCCCGGAGTC CTGGGTAAAC
    GCCACCTCAT CCACCCCCTA TCAGAACATT CCGTGCAATG GATCCAGCAG GCCAGCCCAG
    CCCAGAGAGT TGGCAGCTCC ACCCAAGACC GTCAAAGCCC CCGAGGAACA TCTGAAGCCT
    GAGAGCCTTG AGGGGTCCAG TTCCTTCAAT TACAGCGTGC TGCAGCATCT CGGCCAGTTC
    CCCCCGCTCA TGCCCAACAA GCAGATCGCC GAGTCGGCCA GCAGCAGCAG CCCGCAGAGC
    TCCGGGGGCG GCAAGCCTGC CATGTCCTAC GCCAGCGCCC TGCGGGCCCC TCCAAAGCCC
    AGGCCCCCGC CCGAGCAGGC CAAGAAGAGC AGCGACCCTC TGTCTCTGTT TCAAGAACTC
    AGCCTGGGGA GCTCAGCCGG CAGCAATGGC TTTTACTCCT ATTTTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836905.1
    CDS207..6017
    Translation

    Target ORF information:

    RefSeq Version XM_024981137.1
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase with zinc finger (HELZ), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981137.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGGATTC TCATTTGCAC CCATTCTAAT
    AGTGCTGCTG ATCTCTACAT AAAGGATTAT TTACATCCAT ATGTAGAAGC AGGCAATCCC
    CAGGCAAGAC CTCTCAGGGT ATATTTCAGA AACCGCTGGG TGAAGACTGT CCACCCAGTT
    GTGCATCAGT ACTGTTTGAT CTCAAGCGCA CATTCCACCT TTCAGATGCC ACAGAAAGAA
    GATATTCTGA AACACCGAGT GGTGGTGGTT ACATTGAACA CTTCCCAGTA CCTCTGTCAG
    CTGGACCTCG AACCTGGGTT TTTTACTCAC ATCTTGTTAG ACGAGGCTGC CCAGGCCATG
    GAGTGTGAAA CCATCATGCC TCTGGCACTA GCAACTAGAC ACACGCGGGT TGTCCTGGCT
    GGCGACCACA TGCAGCTCAG TCCTTTTGTT TACAGCGAGT TTGCCAGGGA GAGGAACCTT
    CACGTGTCGT TACTTGATCG GCTCTATGAG CACTACCCTG CCGAGTTTCC CTGTAGGATC
    CTCCTGTGCG AGAACTACCG TTCCCACGAA GCTATCATCA ATTACACCTC TGAGCTCTTC
    TATGAGGGCA AGCTGATGGC CAGTGGAAAG CAACCAGCAC ACAAGGAGTT CTATCCACTA
    ACCTTCTTTA CAGCACGAGG GGAAGATGTA CAGGAGAAAA ATAGCACAGC TTTTTATAAT
    AATGCAGAGG TATTTGAAGT GGTGGAGCGA GTGGAAGAGC TGAGAAGGAA GTGGCCAGTA
    GCGTGGGGCA AGTTGGATGA TGGAAGTATT GGTGTGGTGA CTCCATATGC AGACCAGGTG
    TTCAGGATAC GCGCCGAACT TCGAAAAAAG AGGTTATCTG ACGTTAACGT AGAAAGGGTG
    CTCAATGTTC AAGGAAAGCA ATTCAGAGTT TTGTTTCTTA GCACAGTACG TACAAGGCAT
    ACTTGTAAAC ATAAACAGAC ACCAATTAAA AAGAAGGAGC AGCTGCTAGA AGACTCCACA
    GAGGACTTGG ATTATGGCTT TTTGTCTAAC TACAAGCTCC TCAACACCGC CATCACAAGA
    GCACAGTCCC TGGTTGCCGT GGTGGGGGAC CCCATCGCTC TGTGCTCCAT TGGGAGATGC
    AGGAAATTTT GGGAGCGGTT TATTGCCCTG TGTCATGAAA ACAATAGCCT GCATGGAATC
    ACTTTTGAAC AGATCAAAGC GCAGCTAGAG GCTTTAGAAC TAAAGAAGAC ATACGTGTTG
    AATCCACTGG CACCTGAATT TATACCCCGG GCTCTAAGAG CACAGCACGC GGGAAACACC
    AACAAACCGC AGCAATCACC CCCCAAGGGA AAAAGCCTCC ATCATACCCA GAATGATCAT
    TTCCAGAATG ATGGAATTGT TCAGCCCAAT CCCTCTGTGC TTATTGGCAA TCCTATCAGA
    GCATATACTC CTCCACCTCC TCTTGGACCT CACCCAAATT TGGGGAAATC TCCAAGTCCT
    GTTCAAAGAA TCGATCCTCA CACTGGGACG AGTATTCTTT ATGTACCTGC TGTCTATGGA
    GGGAATGTCG TTATGTCGGT GCCTGTACCT GTGCCATGGA CAGGATACCA GGGCAGGTTT
    GCGGTTGATC CTCGAATCAT TACACACCAG GCAGCAATGG CCTACAACAT GAACCTCTTA
    CAGACACATG GACGTGGGTC TCCTATTCCT TATGGCCTTG GGCACCACCC ACCTGTCAGC
    ATAGGGCAGC CACAGAATCA GCATCAGGAG AAGGATCAAC ATGAGCAAAG TCGCAACGGT
    AAAAGTGATA CAAATAATCC TGGACCTGAA ATTAATAAGA TTCGAACACC AGAGAAAAAA
    CCTACAGAAG CAAAACAGAT TGATTTGGAA TCAAACCCAC AGAACAGAAG TCCTGAATCA
    CGTTCTGGCA TTGTTTATCC CCATCCCAAA TTTCATCGCA AAGATAATCT CACCCCAAGA
    CACATAAACC TTCCTCTCCC TGCCCCCCAT GCCCAGTATG CCATCCCCGG TCGTCATTTT
    CATCCCCTCC CCCAGCTCCC GAGACCACCC TTCCCAATTC CGCAGCAGCA CGCCTTGCTG
    AATCAGCAGC AGAGTAACTT GCCTGAACAG CCGAGCCCCG TGCCAGCCCA GCCCAGTCAG
    GTGGCCCCTC AGCTGGGCCC GCCGCCGCCT CAGCTCTCCC CTGCCTACCC GGCTGGCCCC
    GGCAGCGCGT TTTTCAATAA CGCGGTTCCC CACCGACCAC AGTCCCCGCC TGCAGAGGCT
    GTGGTTCCCG AGCAGCAGCC CCCACCCGTG CTGCAGGAGG GCCACAGTCC CCTGCGGGCC
    CTCGCACAGC CCGGCCCCAT CCTGCCTTCA CCTCTGAACA GCTTCCCGGA CGAGAGCCCC
    CCGGGCCTGC CGATGGGGGA GGCTTTGGAT CGTATGCACG GGAGTGTGGC TCTGGAAACC
    CTGAGGCAGC AGCATGTGCG GCTACAGCAG TGGAGCGAGC ACCACGCCTA TCTCAGTCAG
    GGCAGCCTCC CGTACCCGCA CCATCACCAC CCTCACCCAC ACCTCCAGCA CCTTCCCCAG
    CCGCCCGTTG GATTGCATCA GCCCCCAGTG AGAGCAGACT GGAAACTCCC CAGCAGTGCC
    GAAGATGAAG CAGAAACAAC TGACTCACGG TTTCAAGACT TACTCAGAGA ACTGTCTAAT
    CGTGAGCAAA GTGAAACACG GGAACTAGCT GAAATGCCAC CACCTCAATC AAGGCTTTTG
    CAATATAGAC AAATTCAGAC TAGGAGCCCA CCAGCAGCCC CCTCGCCCCC GTCCGGCGCA
    GACCACAGCA CCCACTTTCC AAACTTCACT GACAGCAGCA GAGACCTTGA AGTGGCCAAC
    AGCCCGGCGT TCCCACAGCG CCTGCCGCCC CCGATGTTTA GCTCTCCTTT CTCGTTGCCA
    TCTGAGCACC TCACCCCTCC TCTGAAATAC CTGGCGCCTG ATGGAGCTTG GACTTTTGCT
    AACTTACAGC AGAATCACCT GATGGGGCCG AGTTTCCCCT ACGGCCTACC TCCATTGCCT
    CACAGGCCCC CGCAGAACCC TTTCGTGCAA ATGCAGACCC ACCAGCCCGC TGTTGGCCAG
    GAGCCGCTCC AGCCGCTGTC CTCTCGGACA GTGTCGTCCT CTTCGCTCCC CAGCTTAGAA
    GAGTATGAAC CCAGAGGACC GGGTCGGCCC TTGTACCAAA GAAGAATCTC ATCTAGCTCA
    GTGCAGCCTT GTTCTGAAGA AGCAAGCACT CCTCAAGACA GTCTTGCTCA GCGTAAAGAG
    CTTCAGGACC ATAATAACCA GTCTTCTTTC ACCTTTTCCT CCCCGGAGTC CTGGGTAAAC
    GCCACCTCAT CCACCCCCTA TCAGAACATT CCGTGCAATG GATCCAGCAG GCCAGCCCAG
    CCCAGAGAGT TGGCAGCTCC ACCCAAGACC GTCAAAGCCC CCGAGGAACA TCTGAAGCCT
    GAGAGCCTTG AGGGGTCCAG TTCCTTCAAT TACAGCGTGC TGCAGCATCT CGGCCAGTTC
    CCCCCGCTCA TGCCCAACAA GCAGATCGCC GAGTCGGCCA GCAGCAGCAG CCCGCAGAGC
    TCCGGGGGCG GCAAGCCTGC CATGTCCTAC GCCAGCGCCC TGCGGGCCCC TCCAAAGCCC
    AGGCCCCCGC CCGAGCAGGC CAAGAAGAGC AGCGACCCTC TGTCTCTGTT TCAAGAACTC
    AGCCTGGGGA GCTCAGCCGG CAGCAATGGC TTTTACTCCT ATTTTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227075
    Clone ID Related Accession (Same CDS sequence) XM_024981137.1 , XM_024981136.1 , XM_024981139.1
    Accession Version XM_024981136.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5811bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable helicase with zinc finger domain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGGATTC TCATTTGCAC CCATTCTAAT
    AGTGCTGCTG ATCTCTACAT AAAGGATTAT TTACATCCAT ATGTAGAAGC AGGCAATCCC
    CAGGCAAGAC CTCTCAGGGT ATATTTCAGA AACCGCTGGG TGAAGACTGT CCACCCAGTT
    GTGCATCAGT ACTGTTTGAT CTCAAGCGCA CATTCCACCT TTCAGATGCC ACAGAAAGAA
    GATATTCTGA AACACCGAGT GGTGGTGGTT ACATTGAACA CTTCCCAGTA CCTCTGTCAG
    CTGGACCTCG AACCTGGGTT TTTTACTCAC ATCTTGTTAG ACGAGGCTGC CCAGGCCATG
    GAGTGTGAAA CCATCATGCC TCTGGCACTA GCAACTAGAC ACACGCGGGT TGTCCTGGCT
    GGCGACCACA TGCAGCTCAG TCCTTTTGTT TACAGCGAGT TTGCCAGGGA GAGGAACCTT
    CACGTGTCGT TACTTGATCG GCTCTATGAG CACTACCCTG CCGAGTTTCC CTGTAGGATC
    CTCCTGTGCG AGAACTACCG TTCCCACGAA GCTATCATCA ATTACACCTC TGAGCTCTTC
    TATGAGGGCA AGCTGATGGC CAGTGGAAAG CAACCAGCAC ACAAGGAGTT CTATCCACTA
    ACCTTCTTTA CAGCACGAGG GGAAGATGTA CAGGAGAAAA ATAGCACAGC TTTTTATAAT
    AATGCAGAGG TATTTGAAGT GGTGGAGCGA GTGGAAGAGC TGAGAAGGAA GTGGCCAGTA
    GCGTGGGGCA AGTTGGATGA TGGAAGTATT GGTGTGGTGA CTCCATATGC AGACCAGGTG
    TTCAGGATAC GCGCCGAACT TCGAAAAAAG AGGTTATCTG ACGTTAACGT AGAAAGGGTG
    CTCAATGTTC AAGGAAAGCA ATTCAGAGTT TTGTTTCTTA GCACAGTACG TACAAGGCAT
    ACTTGTAAAC ATAAACAGAC ACCAATTAAA AAGAAGGAGC AGCTGCTAGA AGACTCCACA
    GAGGACTTGG ATTATGGCTT TTTGTCTAAC TACAAGCTCC TCAACACCGC CATCACAAGA
    GCACAGTCCC TGGTTGCCGT GGTGGGGGAC CCCATCGCTC TGTGCTCCAT TGGGAGATGC
    AGGAAATTTT GGGAGCGGTT TATTGCCCTG TGTCATGAAA ACAATAGCCT GCATGGAATC
    ACTTTTGAAC AGATCAAAGC GCAGCTAGAG GCTTTAGAAC TAAAGAAGAC ATACGTGTTG
    AATCCACTGG CACCTGAATT TATACCCCGG GCTCTAAGAG CACAGCACGC GGGAAACACC
    AACAAACCGC AGCAATCACC CCCCAAGGGA AAAAGCCTCC ATCATACCCA GAATGATCAT
    TTCCAGAATG ATGGAATTGT TCAGCCCAAT CCCTCTGTGC TTATTGGCAA TCCTATCAGA
    GCATATACTC CTCCACCTCC TCTTGGACCT CACCCAAATT TGGGGAAATC TCCAAGTCCT
    GTTCAAAGAA TCGATCCTCA CACTGGGACG AGTATTCTTT ATGTACCTGC TGTCTATGGA
    GGGAATGTCG TTATGTCGGT GCCTGTACCT GTGCCATGGA CAGGATACCA GGGCAGGTTT
    GCGGTTGATC CTCGAATCAT TACACACCAG GCAGCAATGG CCTACAACAT GAACCTCTTA
    CAGACACATG GACGTGGGTC TCCTATTCCT TATGGCCTTG GGCACCACCC ACCTGTCAGC
    ATAGGGCAGC CACAGAATCA GCATCAGGAG AAGGATCAAC ATGAGCAAAG TCGCAACGGT
    AAAAGTGATA CAAATAATCC TGGACCTGAA ATTAATAAGA TTCGAACACC AGAGAAAAAA
    CCTACAGAAG CAAAACAGAT TGATTTGGAA TCAAACCCAC AGAACAGAAG TCCTGAATCA
    CGTTCTGGCA TTGTTTATCC CCATCCCAAA TTTCATCGCA AAGATAATCT CACCCCAAGA
    CACATAAACC TTCCTCTCCC TGCCCCCCAT GCCCAGTATG CCATCCCCGG TCGTCATTTT
    CATCCCCTCC CCCAGCTCCC GAGACCACCC TTCCCAATTC CGCAGCAGCA CGCCTTGCTG
    AATCAGCAGC AGAGTAACTT GCCTGAACAG CCGAGCCCCG TGCCAGCCCA GCCCAGTCAG
    GTGGCCCCTC AGCTGGGCCC GCCGCCGCCT CAGCTCTCCC CTGCCTACCC GGCTGGCCCC
    GGCAGCGCGT TTTTCAATAA CGCGGTTCCC CACCGACCAC AGTCCCCGCC TGCAGAGGCT
    GTGGTTCCCG AGCAGCAGCC CCCACCCGTG CTGCAGGAGG GCCACAGTCC CCTGCGGGCC
    CTCGCACAGC CCGGCCCCAT CCTGCCTTCA CCTCTGAACA GCTTCCCGGA CGAGAGCCCC
    CCGGGCCTGC CGATGGGGGA GGCTTTGGAT CGTATGCACG GGAGTGTGGC TCTGGAAACC
    CTGAGGCAGC AGCATGTGCG GCTACAGCAG TGGAGCGAGC ACCACGCCTA TCTCAGTCAG
    GGCAGCCTCC CGTACCCGCA CCATCACCAC CCTCACCCAC ACCTCCAGCA CCTTCCCCAG
    CCGCCCGTTG GATTGCATCA GCCCCCAGTG AGAGCAGACT GGAAACTCCC CAGCAGTGCC
    GAAGATGAAG CAGAAACAAC TGACTCACGG TTTCAAGACT TACTCAGAGA ACTGTCTAAT
    CGTGAGCAAA GTGAAACACG GGAACTAGCT GAAATGCCAC CACCTCAATC AAGGCTTTTG
    CAATATAGAC AAATTCAGAC TAGGAGCCCA CCAGCAGCCC CCTCGCCCCC GTCCGGCGCA
    GACCACAGCA CCCACTTTCC AAACTTCACT GACAGCAGCA GAGACCTTGA AGTGGCCAAC
    AGCCCGGCGT TCCCACAGCG CCTGCCGCCC CCGATGTTTA GCTCTCCTTT CTCGTTGCCA
    TCTGAGCACC TCACCCCTCC TCTGAAATAC CTGGCGCCTG ATGGAGCTTG GACTTTTGCT
    AACTTACAGC AGAATCACCT GATGGGGCCG AGTTTCCCCT ACGGCCTACC TCCATTGCCT
    CACAGGCCCC CGCAGAACCC TTTCGTGCAA ATGCAGACCC ACCAGCCCGC TGTTGGCCAG
    GAGCCGCTCC AGCCGCTGTC CTCTCGGACA GTGTCGTCCT CTTCGCTCCC CAGCTTAGAA
    GAGTATGAAC CCAGAGGACC GGGTCGGCCC TTGTACCAAA GAAGAATCTC ATCTAGCTCA
    GTGCAGCCTT GTTCTGAAGA AGCAAGCACT CCTCAAGACA GTCTTGCTCA GCGTAAAGAG
    CTTCAGGACC ATAATAACCA GTCTTCTTTC ACCTTTTCCT CCCCGGAGTC CTGGGTAAAC
    GCCACCTCAT CCACCCCCTA TCAGAACATT CCGTGCAATG GATCCAGCAG GCCAGCCCAG
    CCCAGAGAGT TGGCAGCTCC ACCCAAGACC GTCAAAGCCC CCGAGGAACA TCTGAAGCCT
    GAGAGCCTTG AGGGGTCCAG TTCCTTCAAT TACAGCGTGC TGCAGCATCT CGGCCAGTTC
    CCCCCGCTCA TGCCCAACAA GCAGATCGCC GAGTCGGCCA GCAGCAGCAG CCCGCAGAGC
    TCCGGGGGCG GCAAGCCTGC CATGTCCTAC GCCAGCGCCC TGCGGGCCCC TCCAAAGCCC
    AGGCCCCCGC CCGAGCAGGC CAAGAAGAGC AGCGACCCTC TGTCTCTGTT TCAAGAACTC
    AGCCTGGGGA GCTCAGCCGG CAGCAATGGC TTTTACTCCT ATTTTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836904.1
    CDS264..6074
    Translation

    Target ORF information:

    RefSeq Version XM_024981136.1
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase with zinc finger (HELZ), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981136.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGGATTC TCATTTGCAC CCATTCTAAT
    AGTGCTGCTG ATCTCTACAT AAAGGATTAT TTACATCCAT ATGTAGAAGC AGGCAATCCC
    CAGGCAAGAC CTCTCAGGGT ATATTTCAGA AACCGCTGGG TGAAGACTGT CCACCCAGTT
    GTGCATCAGT ACTGTTTGAT CTCAAGCGCA CATTCCACCT TTCAGATGCC ACAGAAAGAA
    GATATTCTGA AACACCGAGT GGTGGTGGTT ACATTGAACA CTTCCCAGTA CCTCTGTCAG
    CTGGACCTCG AACCTGGGTT TTTTACTCAC ATCTTGTTAG ACGAGGCTGC CCAGGCCATG
    GAGTGTGAAA CCATCATGCC TCTGGCACTA GCAACTAGAC ACACGCGGGT TGTCCTGGCT
    GGCGACCACA TGCAGCTCAG TCCTTTTGTT TACAGCGAGT TTGCCAGGGA GAGGAACCTT
    CACGTGTCGT TACTTGATCG GCTCTATGAG CACTACCCTG CCGAGTTTCC CTGTAGGATC
    CTCCTGTGCG AGAACTACCG TTCCCACGAA GCTATCATCA ATTACACCTC TGAGCTCTTC
    TATGAGGGCA AGCTGATGGC CAGTGGAAAG CAACCAGCAC ACAAGGAGTT CTATCCACTA
    ACCTTCTTTA CAGCACGAGG GGAAGATGTA CAGGAGAAAA ATAGCACAGC TTTTTATAAT
    AATGCAGAGG TATTTGAAGT GGTGGAGCGA GTGGAAGAGC TGAGAAGGAA GTGGCCAGTA
    GCGTGGGGCA AGTTGGATGA TGGAAGTATT GGTGTGGTGA CTCCATATGC AGACCAGGTG
    TTCAGGATAC GCGCCGAACT TCGAAAAAAG AGGTTATCTG ACGTTAACGT AGAAAGGGTG
    CTCAATGTTC AAGGAAAGCA ATTCAGAGTT TTGTTTCTTA GCACAGTACG TACAAGGCAT
    ACTTGTAAAC ATAAACAGAC ACCAATTAAA AAGAAGGAGC AGCTGCTAGA AGACTCCACA
    GAGGACTTGG ATTATGGCTT TTTGTCTAAC TACAAGCTCC TCAACACCGC CATCACAAGA
    GCACAGTCCC TGGTTGCCGT GGTGGGGGAC CCCATCGCTC TGTGCTCCAT TGGGAGATGC
    AGGAAATTTT GGGAGCGGTT TATTGCCCTG TGTCATGAAA ACAATAGCCT GCATGGAATC
    ACTTTTGAAC AGATCAAAGC GCAGCTAGAG GCTTTAGAAC TAAAGAAGAC ATACGTGTTG
    AATCCACTGG CACCTGAATT TATACCCCGG GCTCTAAGAG CACAGCACGC GGGAAACACC
    AACAAACCGC AGCAATCACC CCCCAAGGGA AAAAGCCTCC ATCATACCCA GAATGATCAT
    TTCCAGAATG ATGGAATTGT TCAGCCCAAT CCCTCTGTGC TTATTGGCAA TCCTATCAGA
    GCATATACTC CTCCACCTCC TCTTGGACCT CACCCAAATT TGGGGAAATC TCCAAGTCCT
    GTTCAAAGAA TCGATCCTCA CACTGGGACG AGTATTCTTT ATGTACCTGC TGTCTATGGA
    GGGAATGTCG TTATGTCGGT GCCTGTACCT GTGCCATGGA CAGGATACCA GGGCAGGTTT
    GCGGTTGATC CTCGAATCAT TACACACCAG GCAGCAATGG CCTACAACAT GAACCTCTTA
    CAGACACATG GACGTGGGTC TCCTATTCCT TATGGCCTTG GGCACCACCC ACCTGTCAGC
    ATAGGGCAGC CACAGAATCA GCATCAGGAG AAGGATCAAC ATGAGCAAAG TCGCAACGGT
    AAAAGTGATA CAAATAATCC TGGACCTGAA ATTAATAAGA TTCGAACACC AGAGAAAAAA
    CCTACAGAAG CAAAACAGAT TGATTTGGAA TCAAACCCAC AGAACAGAAG TCCTGAATCA
    CGTTCTGGCA TTGTTTATCC CCATCCCAAA TTTCATCGCA AAGATAATCT CACCCCAAGA
    CACATAAACC TTCCTCTCCC TGCCCCCCAT GCCCAGTATG CCATCCCCGG TCGTCATTTT
    CATCCCCTCC CCCAGCTCCC GAGACCACCC TTCCCAATTC CGCAGCAGCA CGCCTTGCTG
    AATCAGCAGC AGAGTAACTT GCCTGAACAG CCGAGCCCCG TGCCAGCCCA GCCCAGTCAG
    GTGGCCCCTC AGCTGGGCCC GCCGCCGCCT CAGCTCTCCC CTGCCTACCC GGCTGGCCCC
    GGCAGCGCGT TTTTCAATAA CGCGGTTCCC CACCGACCAC AGTCCCCGCC TGCAGAGGCT
    GTGGTTCCCG AGCAGCAGCC CCCACCCGTG CTGCAGGAGG GCCACAGTCC CCTGCGGGCC
    CTCGCACAGC CCGGCCCCAT CCTGCCTTCA CCTCTGAACA GCTTCCCGGA CGAGAGCCCC
    CCGGGCCTGC CGATGGGGGA GGCTTTGGAT CGTATGCACG GGAGTGTGGC TCTGGAAACC
    CTGAGGCAGC AGCATGTGCG GCTACAGCAG TGGAGCGAGC ACCACGCCTA TCTCAGTCAG
    GGCAGCCTCC CGTACCCGCA CCATCACCAC CCTCACCCAC ACCTCCAGCA CCTTCCCCAG
    CCGCCCGTTG GATTGCATCA GCCCCCAGTG AGAGCAGACT GGAAACTCCC CAGCAGTGCC
    GAAGATGAAG CAGAAACAAC TGACTCACGG TTTCAAGACT TACTCAGAGA ACTGTCTAAT
    CGTGAGCAAA GTGAAACACG GGAACTAGCT GAAATGCCAC CACCTCAATC AAGGCTTTTG
    CAATATAGAC AAATTCAGAC TAGGAGCCCA CCAGCAGCCC CCTCGCCCCC GTCCGGCGCA
    GACCACAGCA CCCACTTTCC AAACTTCACT GACAGCAGCA GAGACCTTGA AGTGGCCAAC
    AGCCCGGCGT TCCCACAGCG CCTGCCGCCC CCGATGTTTA GCTCTCCTTT CTCGTTGCCA
    TCTGAGCACC TCACCCCTCC TCTGAAATAC CTGGCGCCTG ATGGAGCTTG GACTTTTGCT
    AACTTACAGC AGAATCACCT GATGGGGCCG AGTTTCCCCT ACGGCCTACC TCCATTGCCT
    CACAGGCCCC CGCAGAACCC TTTCGTGCAA ATGCAGACCC ACCAGCCCGC TGTTGGCCAG
    GAGCCGCTCC AGCCGCTGTC CTCTCGGACA GTGTCGTCCT CTTCGCTCCC CAGCTTAGAA
    GAGTATGAAC CCAGAGGACC GGGTCGGCCC TTGTACCAAA GAAGAATCTC ATCTAGCTCA
    GTGCAGCCTT GTTCTGAAGA AGCAAGCACT CCTCAAGACA GTCTTGCTCA GCGTAAAGAG
    CTTCAGGACC ATAATAACCA GTCTTCTTTC ACCTTTTCCT CCCCGGAGTC CTGGGTAAAC
    GCCACCTCAT CCACCCCCTA TCAGAACATT CCGTGCAATG GATCCAGCAG GCCAGCCCAG
    CCCAGAGAGT TGGCAGCTCC ACCCAAGACC GTCAAAGCCC CCGAGGAACA TCTGAAGCCT
    GAGAGCCTTG AGGGGTCCAG TTCCTTCAAT TACAGCGTGC TGCAGCATCT CGGCCAGTTC
    CCCCCGCTCA TGCCCAACAA GCAGATCGCC GAGTCGGCCA GCAGCAGCAG CCCGCAGAGC
    TCCGGGGGCG GCAAGCCTGC CATGTCCTAC GCCAGCGCCC TGCGGGCCCC TCCAAAGCCC
    AGGCCCCCGC CCGAGCAGGC CAAGAAGAGC AGCGACCCTC TGTCTCTGTT TCAAGAACTC
    AGCCTGGGGA GCTCAGCCGG CAGCAATGGC TTTTACTCCT ATTTTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227075
    Clone ID Related Accession (Same CDS sequence) XM_024981137.1 , XM_024981136.1 , XM_024981139.1
    Accession Version XM_024981139.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5811bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable helicase with zinc finger domain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGGATTC TCATTTGCAC CCATTCTAAT
    AGTGCTGCTG ATCTCTACAT AAAGGATTAT TTACATCCAT ATGTAGAAGC AGGCAATCCC
    CAGGCAAGAC CTCTCAGGGT ATATTTCAGA AACCGCTGGG TGAAGACTGT CCACCCAGTT
    GTGCATCAGT ACTGTTTGAT CTCAAGCGCA CATTCCACCT TTCAGATGCC ACAGAAAGAA
    GATATTCTGA AACACCGAGT GGTGGTGGTT ACATTGAACA CTTCCCAGTA CCTCTGTCAG
    CTGGACCTCG AACCTGGGTT TTTTACTCAC ATCTTGTTAG ACGAGGCTGC CCAGGCCATG
    GAGTGTGAAA CCATCATGCC TCTGGCACTA GCAACTAGAC ACACGCGGGT TGTCCTGGCT
    GGCGACCACA TGCAGCTCAG TCCTTTTGTT TACAGCGAGT TTGCCAGGGA GAGGAACCTT
    CACGTGTCGT TACTTGATCG GCTCTATGAG CACTACCCTG CCGAGTTTCC CTGTAGGATC
    CTCCTGTGCG AGAACTACCG TTCCCACGAA GCTATCATCA ATTACACCTC TGAGCTCTTC
    TATGAGGGCA AGCTGATGGC CAGTGGAAAG CAACCAGCAC ACAAGGAGTT CTATCCACTA
    ACCTTCTTTA CAGCACGAGG GGAAGATGTA CAGGAGAAAA ATAGCACAGC TTTTTATAAT
    AATGCAGAGG TATTTGAAGT GGTGGAGCGA GTGGAAGAGC TGAGAAGGAA GTGGCCAGTA
    GCGTGGGGCA AGTTGGATGA TGGAAGTATT GGTGTGGTGA CTCCATATGC AGACCAGGTG
    TTCAGGATAC GCGCCGAACT TCGAAAAAAG AGGTTATCTG ACGTTAACGT AGAAAGGGTG
    CTCAATGTTC AAGGAAAGCA ATTCAGAGTT TTGTTTCTTA GCACAGTACG TACAAGGCAT
    ACTTGTAAAC ATAAACAGAC ACCAATTAAA AAGAAGGAGC AGCTGCTAGA AGACTCCACA
    GAGGACTTGG ATTATGGCTT TTTGTCTAAC TACAAGCTCC TCAACACCGC CATCACAAGA
    GCACAGTCCC TGGTTGCCGT GGTGGGGGAC CCCATCGCTC TGTGCTCCAT TGGGAGATGC
    AGGAAATTTT GGGAGCGGTT TATTGCCCTG TGTCATGAAA ACAATAGCCT GCATGGAATC
    ACTTTTGAAC AGATCAAAGC GCAGCTAGAG GCTTTAGAAC TAAAGAAGAC ATACGTGTTG
    AATCCACTGG CACCTGAATT TATACCCCGG GCTCTAAGAG CACAGCACGC GGGAAACACC
    AACAAACCGC AGCAATCACC CCCCAAGGGA AAAAGCCTCC ATCATACCCA GAATGATCAT
    TTCCAGAATG ATGGAATTGT TCAGCCCAAT CCCTCTGTGC TTATTGGCAA TCCTATCAGA
    GCATATACTC CTCCACCTCC TCTTGGACCT CACCCAAATT TGGGGAAATC TCCAAGTCCT
    GTTCAAAGAA TCGATCCTCA CACTGGGACG AGTATTCTTT ATGTACCTGC TGTCTATGGA
    GGGAATGTCG TTATGTCGGT GCCTGTACCT GTGCCATGGA CAGGATACCA GGGCAGGTTT
    GCGGTTGATC CTCGAATCAT TACACACCAG GCAGCAATGG CCTACAACAT GAACCTCTTA
    CAGACACATG GACGTGGGTC TCCTATTCCT TATGGCCTTG GGCACCACCC ACCTGTCAGC
    ATAGGGCAGC CACAGAATCA GCATCAGGAG AAGGATCAAC ATGAGCAAAG TCGCAACGGT
    AAAAGTGATA CAAATAATCC TGGACCTGAA ATTAATAAGA TTCGAACACC AGAGAAAAAA
    CCTACAGAAG CAAAACAGAT TGATTTGGAA TCAAACCCAC AGAACAGAAG TCCTGAATCA
    CGTTCTGGCA TTGTTTATCC CCATCCCAAA TTTCATCGCA AAGATAATCT CACCCCAAGA
    CACATAAACC TTCCTCTCCC TGCCCCCCAT GCCCAGTATG CCATCCCCGG TCGTCATTTT
    CATCCCCTCC CCCAGCTCCC GAGACCACCC TTCCCAATTC CGCAGCAGCA CGCCTTGCTG
    AATCAGCAGC AGAGTAACTT GCCTGAACAG CCGAGCCCCG TGCCAGCCCA GCCCAGTCAG
    GTGGCCCCTC AGCTGGGCCC GCCGCCGCCT CAGCTCTCCC CTGCCTACCC GGCTGGCCCC
    GGCAGCGCGT TTTTCAATAA CGCGGTTCCC CACCGACCAC AGTCCCCGCC TGCAGAGGCT
    GTGGTTCCCG AGCAGCAGCC CCCACCCGTG CTGCAGGAGG GCCACAGTCC CCTGCGGGCC
    CTCGCACAGC CCGGCCCCAT CCTGCCTTCA CCTCTGAACA GCTTCCCGGA CGAGAGCCCC
    CCGGGCCTGC CGATGGGGGA GGCTTTGGAT CGTATGCACG GGAGTGTGGC TCTGGAAACC
    CTGAGGCAGC AGCATGTGCG GCTACAGCAG TGGAGCGAGC ACCACGCCTA TCTCAGTCAG
    GGCAGCCTCC CGTACCCGCA CCATCACCAC CCTCACCCAC ACCTCCAGCA CCTTCCCCAG
    CCGCCCGTTG GATTGCATCA GCCCCCAGTG AGAGCAGACT GGAAACTCCC CAGCAGTGCC
    GAAGATGAAG CAGAAACAAC TGACTCACGG TTTCAAGACT TACTCAGAGA ACTGTCTAAT
    CGTGAGCAAA GTGAAACACG GGAACTAGCT GAAATGCCAC CACCTCAATC AAGGCTTTTG
    CAATATAGAC AAATTCAGAC TAGGAGCCCA CCAGCAGCCC CCTCGCCCCC GTCCGGCGCA
    GACCACAGCA CCCACTTTCC AAACTTCACT GACAGCAGCA GAGACCTTGA AGTGGCCAAC
    AGCCCGGCGT TCCCACAGCG CCTGCCGCCC CCGATGTTTA GCTCTCCTTT CTCGTTGCCA
    TCTGAGCACC TCACCCCTCC TCTGAAATAC CTGGCGCCTG ATGGAGCTTG GACTTTTGCT
    AACTTACAGC AGAATCACCT GATGGGGCCG AGTTTCCCCT ACGGCCTACC TCCATTGCCT
    CACAGGCCCC CGCAGAACCC TTTCGTGCAA ATGCAGACCC ACCAGCCCGC TGTTGGCCAG
    GAGCCGCTCC AGCCGCTGTC CTCTCGGACA GTGTCGTCCT CTTCGCTCCC CAGCTTAGAA
    GAGTATGAAC CCAGAGGACC GGGTCGGCCC TTGTACCAAA GAAGAATCTC ATCTAGCTCA
    GTGCAGCCTT GTTCTGAAGA AGCAAGCACT CCTCAAGACA GTCTTGCTCA GCGTAAAGAG
    CTTCAGGACC ATAATAACCA GTCTTCTTTC ACCTTTTCCT CCCCGGAGTC CTGGGTAAAC
    GCCACCTCAT CCACCCCCTA TCAGAACATT CCGTGCAATG GATCCAGCAG GCCAGCCCAG
    CCCAGAGAGT TGGCAGCTCC ACCCAAGACC GTCAAAGCCC CCGAGGAACA TCTGAAGCCT
    GAGAGCCTTG AGGGGTCCAG TTCCTTCAAT TACAGCGTGC TGCAGCATCT CGGCCAGTTC
    CCCCCGCTCA TGCCCAACAA GCAGATCGCC GAGTCGGCCA GCAGCAGCAG CCCGCAGAGC
    TCCGGGGGCG GCAAGCCTGC CATGTCCTAC GCCAGCGCCC TGCGGGCCCC TCCAAAGCCC
    AGGCCCCCGC CCGAGCAGGC CAAGAAGAGC AGCGACCCTC TGTCTCTGTT TCAAGAACTC
    AGCCTGGGGA GCTCAGCCGG CAGCAATGGC TTTTACTCCT ATTTTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836907.1
    CDS113..5923
    Translation

    Target ORF information:

    RefSeq Version XM_024981139.1
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase with zinc finger (HELZ), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981139.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAAACA ACAGCTAGTA
    ACTACAGCTA AACGTTGGGA TTCTTCTTCT AAGACTATTG TAGATTTTGA ACCTAATGAA
    ACTACTGATT TGGAGAAGAG CCTTCTTATC AGATACCAAA TTCCTCTCTC TGCTGACCAG
    CTGTTTACCC AGTCCGTTTT AGACAAATCA TTGACCAAGA CCAACTATCA GTCACGGTTG
    CATGACCTTC TTTATATTGA GGAGATAGCC CAGTATAAGG AAGTCAGCAA GTTCAATCTT
    AAAGTGCAGT TGCAGATTCT AGCAAGCTTC ATGCTCACTG GAGTTTCTGG AGGTGCAAAG
    TACGCTCAGA ATGGACAACT TTTTGGTCGC TTTAAGCTTA CTGAAACTCT TTCTGAAGAT
    ACTTTGGCTG GACGGCTGGT GATGACCAAA GTCAATGCTG TTTATTTATT ACCAGTCCCT
    AAAGAAAAGT TAGTACAGAC CCAGGGAACC AAAGAGAAGG TTTATGAAGC TACTATTGAA
    GAAAAAACAA AAGAATATAT ATTTTTAAGG ATATCCAGGG AATGCTGTGA AGAACTTAAT
    CTTCGGCCTG ATTACGACAC ACAGGTTGAA CTCCAGTTTC AGTTAAATCG ATTACCCCTC
    TGTGAAATGC ATTATGCGCT AGACAGGGTC AAGGACAATG GGGTTTTGTT TCCAGACATC
    ACTATGGCTC CAACCATACC TTGGAGTCCC AACAGGCAAT GGGACGAACA GTTGGATCCT
    CGGCTAAATG CAAAACAGAA AGAGGCTGTT CTGGCCATTA CCACCCCACT TTCAATACAG
    TTGCCTCCTG TCCTCATCAT CGGCCCATAC GGGACGGGCA AAACATTCAC TCTAGCTCAG
    GCCGTCAAGC ACATTCTCCA GCAACAGGAG ACCAGGATTC TCATTTGCAC CCATTCTAAT
    AGTGCTGCTG ATCTCTACAT AAAGGATTAT TTACATCCAT ATGTAGAAGC AGGCAATCCC
    CAGGCAAGAC CTCTCAGGGT ATATTTCAGA AACCGCTGGG TGAAGACTGT CCACCCAGTT
    GTGCATCAGT ACTGTTTGAT CTCAAGCGCA CATTCCACCT TTCAGATGCC ACAGAAAGAA
    GATATTCTGA AACACCGAGT GGTGGTGGTT ACATTGAACA CTTCCCAGTA CCTCTGTCAG
    CTGGACCTCG AACCTGGGTT TTTTACTCAC ATCTTGTTAG ACGAGGCTGC CCAGGCCATG
    GAGTGTGAAA CCATCATGCC TCTGGCACTA GCAACTAGAC ACACGCGGGT TGTCCTGGCT
    GGCGACCACA TGCAGCTCAG TCCTTTTGTT TACAGCGAGT TTGCCAGGGA GAGGAACCTT
    CACGTGTCGT TACTTGATCG GCTCTATGAG CACTACCCTG CCGAGTTTCC CTGTAGGATC
    CTCCTGTGCG AGAACTACCG TTCCCACGAA GCTATCATCA ATTACACCTC TGAGCTCTTC
    TATGAGGGCA AGCTGATGGC CAGTGGAAAG CAACCAGCAC ACAAGGAGTT CTATCCACTA
    ACCTTCTTTA CAGCACGAGG GGAAGATGTA CAGGAGAAAA ATAGCACAGC TTTTTATAAT
    AATGCAGAGG TATTTGAAGT GGTGGAGCGA GTGGAAGAGC TGAGAAGGAA GTGGCCAGTA
    GCGTGGGGCA AGTTGGATGA TGGAAGTATT GGTGTGGTGA CTCCATATGC AGACCAGGTG
    TTCAGGATAC GCGCCGAACT TCGAAAAAAG AGGTTATCTG ACGTTAACGT AGAAAGGGTG
    CTCAATGTTC AAGGAAAGCA ATTCAGAGTT TTGTTTCTTA GCACAGTACG TACAAGGCAT
    ACTTGTAAAC ATAAACAGAC ACCAATTAAA AAGAAGGAGC AGCTGCTAGA AGACTCCACA
    GAGGACTTGG ATTATGGCTT TTTGTCTAAC TACAAGCTCC TCAACACCGC CATCACAAGA
    GCACAGTCCC TGGTTGCCGT GGTGGGGGAC CCCATCGCTC TGTGCTCCAT TGGGAGATGC
    AGGAAATTTT GGGAGCGGTT TATTGCCCTG TGTCATGAAA ACAATAGCCT GCATGGAATC
    ACTTTTGAAC AGATCAAAGC GCAGCTAGAG GCTTTAGAAC TAAAGAAGAC ATACGTGTTG
    AATCCACTGG CACCTGAATT TATACCCCGG GCTCTAAGAG CACAGCACGC GGGAAACACC
    AACAAACCGC AGCAATCACC CCCCAAGGGA AAAAGCCTCC ATCATACCCA GAATGATCAT
    TTCCAGAATG ATGGAATTGT TCAGCCCAAT CCCTCTGTGC TTATTGGCAA TCCTATCAGA
    GCATATACTC CTCCACCTCC TCTTGGACCT CACCCAAATT TGGGGAAATC TCCAAGTCCT
    GTTCAAAGAA TCGATCCTCA CACTGGGACG AGTATTCTTT ATGTACCTGC TGTCTATGGA
    GGGAATGTCG TTATGTCGGT GCCTGTACCT GTGCCATGGA CAGGATACCA GGGCAGGTTT
    GCGGTTGATC CTCGAATCAT TACACACCAG GCAGCAATGG CCTACAACAT GAACCTCTTA
    CAGACACATG GACGTGGGTC TCCTATTCCT TATGGCCTTG GGCACCACCC ACCTGTCAGC
    ATAGGGCAGC CACAGAATCA GCATCAGGAG AAGGATCAAC ATGAGCAAAG TCGCAACGGT
    AAAAGTGATA CAAATAATCC TGGACCTGAA ATTAATAAGA TTCGAACACC AGAGAAAAAA
    CCTACAGAAG CAAAACAGAT TGATTTGGAA TCAAACCCAC AGAACAGAAG TCCTGAATCA
    CGTTCTGGCA TTGTTTATCC CCATCCCAAA TTTCATCGCA AAGATAATCT CACCCCAAGA
    CACATAAACC TTCCTCTCCC TGCCCCCCAT GCCCAGTATG CCATCCCCGG TCGTCATTTT
    CATCCCCTCC CCCAGCTCCC GAGACCACCC TTCCCAATTC CGCAGCAGCA CGCCTTGCTG
    AATCAGCAGC AGAGTAACTT GCCTGAACAG CCGAGCCCCG TGCCAGCCCA GCCCAGTCAG
    GTGGCCCCTC AGCTGGGCCC GCCGCCGCCT CAGCTCTCCC CTGCCTACCC GGCTGGCCCC
    GGCAGCGCGT TTTTCAATAA CGCGGTTCCC CACCGACCAC AGTCCCCGCC TGCAGAGGCT
    GTGGTTCCCG AGCAGCAGCC CCCACCCGTG CTGCAGGAGG GCCACAGTCC CCTGCGGGCC
    CTCGCACAGC CCGGCCCCAT CCTGCCTTCA CCTCTGAACA GCTTCCCGGA CGAGAGCCCC
    CCGGGCCTGC CGATGGGGGA GGCTTTGGAT CGTATGCACG GGAGTGTGGC TCTGGAAACC
    CTGAGGCAGC AGCATGTGCG GCTACAGCAG TGGAGCGAGC ACCACGCCTA TCTCAGTCAG
    GGCAGCCTCC CGTACCCGCA CCATCACCAC CCTCACCCAC ACCTCCAGCA CCTTCCCCAG
    CCGCCCGTTG GATTGCATCA GCCCCCAGTG AGAGCAGACT GGAAACTCCC CAGCAGTGCC
    GAAGATGAAG CAGAAACAAC TGACTCACGG TTTCAAGACT TACTCAGAGA ACTGTCTAAT
    CGTGAGCAAA GTGAAACACG GGAACTAGCT GAAATGCCAC CACCTCAATC AAGGCTTTTG
    CAATATAGAC AAATTCAGAC TAGGAGCCCA CCAGCAGCCC CCTCGCCCCC GTCCGGCGCA
    GACCACAGCA CCCACTTTCC AAACTTCACT GACAGCAGCA GAGACCTTGA AGTGGCCAAC
    AGCCCGGCGT TCCCACAGCG CCTGCCGCCC CCGATGTTTA GCTCTCCTTT CTCGTTGCCA
    TCTGAGCACC TCACCCCTCC TCTGAAATAC CTGGCGCCTG ATGGAGCTTG GACTTTTGCT
    AACTTACAGC AGAATCACCT GATGGGGCCG AGTTTCCCCT ACGGCCTACC TCCATTGCCT
    CACAGGCCCC CGCAGAACCC TTTCGTGCAA ATGCAGACCC ACCAGCCCGC TGTTGGCCAG
    GAGCCGCTCC AGCCGCTGTC CTCTCGGACA GTGTCGTCCT CTTCGCTCCC CAGCTTAGAA
    GAGTATGAAC CCAGAGGACC GGGTCGGCCC TTGTACCAAA GAAGAATCTC ATCTAGCTCA
    GTGCAGCCTT GTTCTGAAGA AGCAAGCACT CCTCAAGACA GTCTTGCTCA GCGTAAAGAG
    CTTCAGGACC ATAATAACCA GTCTTCTTTC ACCTTTTCCT CCCCGGAGTC CTGGGTAAAC
    GCCACCTCAT CCACCCCCTA TCAGAACATT CCGTGCAATG GATCCAGCAG GCCAGCCCAG
    CCCAGAGAGT TGGCAGCTCC ACCCAAGACC GTCAAAGCCC CCGAGGAACA TCTGAAGCCT
    GAGAGCCTTG AGGGGTCCAG TTCCTTCAAT TACAGCGTGC TGCAGCATCT CGGCCAGTTC
    CCCCCGCTCA TGCCCAACAA GCAGATCGCC GAGTCGGCCA GCAGCAGCAG CCCGCAGAGC
    TCCGGGGGCG GCAAGCCTGC CATGTCCTAC GCCAGCGCCC TGCGGGCCCC TCCAAAGCCC
    AGGCCCCCGC CCGAGCAGGC CAAGAAGAGC AGCGACCCTC TGTCTCTGTT TCAAGAACTC
    AGCCTGGGGA GCTCAGCCGG CAGCAATGGC TTTTACTCCT ATTTTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227076
    Clone ID Related Accession (Same CDS sequence) XM_024981140.1
    Accession Version XM_024981140.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable helicase with zinc finger domain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGCTCAGTGA GTGTGTGGAA GGAGTGAAGG TGGAGCATGA CCCCGGTCTG
    TCTGTCACCG TCAACACCAA AAAGTCCCAC CAGACCTGGA CCTTCGCTCT CACCTGTAAG
    CCTGCAAGAA TGCTGTGTCG TGTAGCATTG CTTTATGATG CTCATCGTCC TCATTTTAGT
    ATCATTGCAA TATCTGCCGG AGATAGTACT ACCCAGGTAT CACAAGAAGT CCCAGAAAAC
    TGTCAAGAAT GGATAGGAGG GAAGATGGCC CCAAATGGAT TAGATCATTA CGTGTATAAA
    GTCGGGATAG CATTTAACAC AGAAATATTT GGAACTTTTC GCCAAACCAT AGTTTTCGAC
    TTTGGATTGG AACCAGTACT CATGCAAAGA GTAATGATTG ATGCAGCTTC TACAGAAGAT
    CTTGAGTACC TGATGCATGC AAAACAACAG CTAGTAACTA CAGCTAAACG TTGGGATTCT
    TCTTCTAAGA CTATTGTAGA TTTTGAACCT AATGAAACTA CTGATTTGGA GAAGAGCCTT
    CTTATCAGAT ACCAAATTCC TCTCTCTGCT GACCAGCTGT TTACCCAGTC CGTTTTAGAC
    AAATCATTGA CCAAGACCAA CTATCAGTCA CGGTTGCATG ACCTTCTTTA TATTGAGGAG
    ATAGCCCAGT ATAAGGAAGT CAGCAAGTTC AATCTTAAAG TGCAGTTGCA GATTCTAGCA
    AGCTTCATGC TCACTGGAGT TTCTGGAGGT GCAAAGTACG CTCAGAATGG ACAACTTTTT
    GGTCGCTTTA AGCTTACTGA AACTCTTTCT GAAGATACTT TGGCTGGACG GCTGGTGATG
    ACCAAAGTCA ATGCTGTTTA TTTATTACCA GTCCCTAAAG AAAAGTTAGT ACAGACCCAG
    GGAACCAAAG AGAAGGTTTA TGAAGCTACT ATTGAAGAAA AAACAAAAGA ATATATATTT
    TTAAGGATAT CCAGGGAATG CTGTGAAGAA CTTAATCTTC GGCCTGATTA CGACACACAG
    GTTGAACTCC AGTTTCAGTT AAATCGATTA CCCCTCTGTG AAATGCATTA TGCGCTAGAC
    AGGGTCAAGG ACAATGGGGT TTTGTTTCCA GACATCACTA TGGCTCCAAC CATACCTTGG
    AGTCCCAACA GGCAATGGGA CGAACAGTTG GATCCTCGGC TAAATGCAAA ACAGAAAGAG
    GCTGTTCTGG CCATTACCAC CCCACTTTCA ATACAGTTGC CTCCTGTCCT CATCATCGGC
    CCATACGGGA CGGGCAAAAC ATTCACTCTA GCTCAGGCCG TCAAGCACAT TCTCCAGCAA
    CAGGAGACCA GCAGGATTCT CATTTGCACC CATTCTAATA GTGCTGCTGA TCTCTACATA
    AAGGATTATT TACATCCATA TGTAGAAGCA GGCAATCCCC AGGCAAGACC TCTCAGGGTA
    TATTTCAGAA ACCGCTGGGT GAAGACTGTC CACCCAGTTG TGCATCAGTA CTGTTTGATC
    TCAAGCGCAC ATTCCACCTT TCAGATGCCA CAGAAAGAAG ATATTCTGAA ACACCGAGTG
    GTGGTGGTTA CATTGAACAC TTCCCAGTAC CTCTGTCAGC TGGACCTCGA ACCTGGGTTT
    TTTACTCACA TCTTGTTAGA CGAGGCTGCC CAGGCCATGG AGTGTGAAAC CATCATGCCT
    CTGGCACTAG CAACTAGACA CACGCGGGTT GTCCTGGCTG GCGACCACAT GCAGCTCAGT
    CCTTTTGTTT ACAGCGAGTT TGCCAGGGAG AGGAACCTTC ACGTGTCGTT ACTTGATCGG
    CTCTATGAGC ACTACCCTGC CGAGTTTCCC TGTAGGATCC TCCTGTGCGA GAACTACCGT
    TCCCACGAAG CTATCATCAA TTACACCTCT GAGCTCTTCT ATGAGGGCAA GCTGATGGCC
    AGTGGAAAGC AACCAGCACA CAAGGAGTTC TATCCACTAA CCTTCTTTAC AGCACGAGGG
    GAAGATGTAC AGGAGAAAAA TAGCACAGCT TTTTATAATA ATGCAGAGGT ATTTGAAGTG
    GTGGAGCGAG TGGAAGAGCT GAGAAGGAAG TGGCCAGTAG CGTGGGGCAA GTTGGATGAT
    GGAAGTATTG GTGTGGTGAC TCCATATGCA GACCAGGTGT TCAGGATACG CGCCGAACTT
    CGAAAAAAGA GGTTATCTGA CGTTAACGTA GAAAGGGTGC TCAATGTTCA AGGAAAGCAA
    TTCAGAGTTT TGTTTCTTAG CACAGTACGT ACAAGGCATA CTTGTAAACA TAAACAGACA
    CCAATTAAAA AGAAGGAGCA GCTGCTAGAA GACTCCACAG AGGACTTGGA TTATGGCTTT
    TTGTCTAACT ACAAGCTCCT CAACACCGCC ATCACAAGAG CACAGTCCCT GGTTGCCGTG
    GTGGGGGACC CCATCGCTCT GTGCTCCATT GGGAGATGCA GGAAATTTTG GGAGCGGTTT
    ATTGCCCTGT GTCATGAAAA CAATAGCCTG CATGGAATCA CTTTTGAACA GATCAAAGCG
    CAGCTAGAGG CTTTAGAACT AAAGAAGACA TACGTGTTGA ATCCACTGGC ACCTGAATTT
    ATACCCCGGG CTCTAAGAGC ACAGCACGCG GGAAACACCA ACAAACCGCA GCAATCACCC
    CCCAAGGGAA AAAGCCTCCA TCATACCCAG AATGATCATT TCCAGAATGA TGGAATTGTT
    CAGCCCAATC CCTCTGTGCT TATTGGCAAT CCTATCAGAG CATATACTCC TCCACCTCCT
    CTTGGACCTC ACCCAAATTT GGGGAAATCT CCAAGTCCTG TTCAAAGAAT CGATCCTCAC
    ACTGGGACGA GTATTCTTTA TGTACCTGCT GTCTATGGAG GGAATGTCGT TATGTCGGTG
    CCTGTACCTG TGCCATGGAC AGGATACCAG GGCAGGTTTG CGGTTGATCC TCGAATCATT
    ACACACCAGG CAGCAATGGC CTACAACATG AACCTCTTAC AGACACATGG ACGTGGGTCT
    CCTATTCCTT ATGGCCTTGG GCACCACCCA CCTGTCAGCA TAGGGCAGCC ACAGAATCAG
    CATCAGGAGA AGGATCAACA TGAGCAAAGT CGCAACGGTA AAAGTGATAC AAATAATCCT
    GGACCTGAAA TTAATAAGAT TCGAACACCA GAGAAAAAAC CTACAGAAGC AAAACAGATT
    GATTTGGAAT CAAACCCACA GAACAGAAGT CCTGAATCAC GTTCTGGCAT TGTTTATCCC
    CATCCCAAAT TTCATCGCAA AGATAATCTC ACCCCAAGAC ACATAAACCT TCCTCTCCCT
    GCCCCCCATG CCCAGTATGC CATCCCCGGT CGTCATTTTC ATCCCCTCCC CCAGCTCCCG
    AGACCACCCT TCCCAATTCC GCAGCAGCAC GCCTTGCTGA ATCAGCAGCA GAGTAACTTG
    CCTGAACAGC CGAGCCCCGT GCCAGCCCAG CCCAGTCAGG TGGCCCCTCA GCTGGGCCCG
    CCGCCGCCTC AGCTCTCCCC TGCCTACCCG GCTGGCCCCG GCAGCGCGTT TTTCAATAAC
    GCGGTTCCCC ACCGACCACA GTCCCCGCCT GCAGAGGCTG TGGTTCCCGA GCAGCAGCCC
    CCACCCGTGC TGCAGGAGGG CCACAGTCCC CTGCGGGCCC TCGCACAGCC CGGCCCCATC
    CTGCCTTCAC CTCTGAACAG CTTCCCGGAC GAGAGCCCCC CGGGCCTGCC GATGGGGGAG
    GCTTTGGATC GTATGCACGG GAGTGTGGCT CTGGAAACCC TGAGGCAGCA GCATGTGCGG
    CTACAGCAGT GGAGCGAGCA CCACGCCTAT CTCAGTCAGG GCAGCCTCCC GTACCCGCAC
    CATCACCACC CTCACCCACA CCTCCAGCAC CTTCCCCAGC CGCCCGTTGG ATTGCATCAG
    CCCCCAGTGA GAGCAGACTG GAAACTCCCC AGCAGTGCCG AAGATGAAGC AGAAACAACT
    GACTCACGGT TTCAAGACTT ACTCAGAGAA CTGTCTAATC GTGAGCAAAG TGAAACACGG
    GAACTAGCTG AAATGCCACC ACCTCAATCA AGGCTTTTGC AATATAGACA AATTCAGACT
    AGGAGCCCAC CAGCAGCCCC CTCGCCCCCG TCCGGCGCAG ACCACAGCAC CCACTTTCCA
    AACTTCACTG ACAGCAGCAG AGACCTTGAA GTGGCCAACA GCCCGGCGTT CCCACAGCGC
    CTGCCGCCCC CGATGTTTAG CTCTCCTTTC TCGTTGCCAT CTGAGCACCT CACCCCTCCT
    CTGAAATACC TGGCGCCTGA TGGAGCTTGG ACTTTTGCTA ACTTACAGCA GAATCACCTG
    ATGGGGCCGA GTTTCCCCTA CGGCCTACCT CCATTGCCTC ACAGGCCCCC GCAGAACCCT
    TTCGTGCAAA TGCAGACCCA CCAGCCCGCT GTTGGCCAGG AGCCGCTCCA GCCGCTGTCC
    TCTCGGACAG TGTCGTCCTC TTCGCTCCCC AGCTTAGAAG AGTATGAACC CAGAGGACCG
    GGTCGGCCCT TGTACCAAAG AAGAATCTCA TCTAGCTCAG TGCAGCCTTG TTCTGAAGAA
    GCAAGCACTC CTCAAGACAG TCTTGCTCAG CGTAAAGAGC TTCAGGACCA TAATAACCAG
    TCTTCTTTCA CCTTTTCCTC CCCGGAGTCC TGGGTAAACG CCACCTCATC CACCCCCTAT
    CAGAACATTC CGTGCAATGG ATCCAGCAGG CCAGCCCAGC CCAGAGAGTT GGCAGCTCCA
    CCCAAGACCG TCAAAGCCCC CGAGGAACAT CTGAAGCCTG AGAGCCTTGA GGGGTCCAGT
    TCCTTCAATT ACAGCGTGCT GCAGCATCTC GGCCAGTTCC CCCCGCTCAT GCCCAACAAG
    CAGATCGCCG AGTCGGCCAG CAGCAGCAGC CCGCAGAGCT CCGGGGGCGG CAAGCCTGCC
    ATGTCCTACG CCAGCGCCCT GCGGGCCCCT CCAAAGCCCA GGCCCCCGCC CGAGCAGGCC
    AAGAAGAGCA GCGACCCTCT GTCTCTGTTT CAAGAACTCA GCCTGGGGAG CTCAGCCGGC
    AGCAATGGCT TTTACTCCTA TTTTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836908.1
    CDS264..5693
    Translation

    Target ORF information:

    RefSeq Version XM_024981140.1
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase with zinc finger (HELZ), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981140.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGCTCAGTGA GTGTGTGGAA GGAGTGAAGG TGGAGCATGA CCCCGGTCTG
    TCTGTCACCG TCAACACCAA AAAGTCCCAC CAGACCTGGA CCTTCGCTCT CACCTGTAAG
    CCTGCAAGAA TGCTGTGTCG TGTAGCATTG CTTTATGATG CTCATCGTCC TCATTTTAGT
    ATCATTGCAA TATCTGCCGG AGATAGTACT ACCCAGGTAT CACAAGAAGT CCCAGAAAAC
    TGTCAAGAAT GGATAGGAGG GAAGATGGCC CCAAATGGAT TAGATCATTA CGTGTATAAA
    GTCGGGATAG CATTTAACAC AGAAATATTT GGAACTTTTC GCCAAACCAT AGTTTTCGAC
    TTTGGATTGG AACCAGTACT CATGCAAAGA GTAATGATTG ATGCAGCTTC TACAGAAGAT
    CTTGAGTACC TGATGCATGC AAAACAACAG CTAGTAACTA CAGCTAAACG TTGGGATTCT
    TCTTCTAAGA CTATTGTAGA TTTTGAACCT AATGAAACTA CTGATTTGGA GAAGAGCCTT
    CTTATCAGAT ACCAAATTCC TCTCTCTGCT GACCAGCTGT TTACCCAGTC CGTTTTAGAC
    AAATCATTGA CCAAGACCAA CTATCAGTCA CGGTTGCATG ACCTTCTTTA TATTGAGGAG
    ATAGCCCAGT ATAAGGAAGT CAGCAAGTTC AATCTTAAAG TGCAGTTGCA GATTCTAGCA
    AGCTTCATGC TCACTGGAGT TTCTGGAGGT GCAAAGTACG CTCAGAATGG ACAACTTTTT
    GGTCGCTTTA AGCTTACTGA AACTCTTTCT GAAGATACTT TGGCTGGACG GCTGGTGATG
    ACCAAAGTCA ATGCTGTTTA TTTATTACCA GTCCCTAAAG AAAAGTTAGT ACAGACCCAG
    GGAACCAAAG AGAAGGTTTA TGAAGCTACT ATTGAAGAAA AAACAAAAGA ATATATATTT
    TTAAGGATAT CCAGGGAATG CTGTGAAGAA CTTAATCTTC GGCCTGATTA CGACACACAG
    GTTGAACTCC AGTTTCAGTT AAATCGATTA CCCCTCTGTG AAATGCATTA TGCGCTAGAC
    AGGGTCAAGG ACAATGGGGT TTTGTTTCCA GACATCACTA TGGCTCCAAC CATACCTTGG
    AGTCCCAACA GGCAATGGGA CGAACAGTTG GATCCTCGGC TAAATGCAAA ACAGAAAGAG
    GCTGTTCTGG CCATTACCAC CCCACTTTCA ATACAGTTGC CTCCTGTCCT CATCATCGGC
    CCATACGGGA CGGGCAAAAC ATTCACTCTA GCTCAGGCCG TCAAGCACAT TCTCCAGCAA
    CAGGAGACCA GCAGGATTCT CATTTGCACC CATTCTAATA GTGCTGCTGA TCTCTACATA
    AAGGATTATT TACATCCATA TGTAGAAGCA GGCAATCCCC AGGCAAGACC TCTCAGGGTA
    TATTTCAGAA ACCGCTGGGT GAAGACTGTC CACCCAGTTG TGCATCAGTA CTGTTTGATC
    TCAAGCGCAC ATTCCACCTT TCAGATGCCA CAGAAAGAAG ATATTCTGAA ACACCGAGTG
    GTGGTGGTTA CATTGAACAC TTCCCAGTAC CTCTGTCAGC TGGACCTCGA ACCTGGGTTT
    TTTACTCACA TCTTGTTAGA CGAGGCTGCC CAGGCCATGG AGTGTGAAAC CATCATGCCT
    CTGGCACTAG CAACTAGACA CACGCGGGTT GTCCTGGCTG GCGACCACAT GCAGCTCAGT
    CCTTTTGTTT ACAGCGAGTT TGCCAGGGAG AGGAACCTTC ACGTGTCGTT ACTTGATCGG
    CTCTATGAGC ACTACCCTGC CGAGTTTCCC TGTAGGATCC TCCTGTGCGA GAACTACCGT
    TCCCACGAAG CTATCATCAA TTACACCTCT GAGCTCTTCT ATGAGGGCAA GCTGATGGCC
    AGTGGAAAGC AACCAGCACA CAAGGAGTTC TATCCACTAA CCTTCTTTAC AGCACGAGGG
    GAAGATGTAC AGGAGAAAAA TAGCACAGCT TTTTATAATA ATGCAGAGGT ATTTGAAGTG
    GTGGAGCGAG TGGAAGAGCT GAGAAGGAAG TGGCCAGTAG CGTGGGGCAA GTTGGATGAT
    GGAAGTATTG GTGTGGTGAC TCCATATGCA GACCAGGTGT TCAGGATACG CGCCGAACTT
    CGAAAAAAGA GGTTATCTGA CGTTAACGTA GAAAGGGTGC TCAATGTTCA AGGAAAGCAA
    TTCAGAGTTT TGTTTCTTAG CACAGTACGT ACAAGGCATA CTTGTAAACA TAAACAGACA
    CCAATTAAAA AGAAGGAGCA GCTGCTAGAA GACTCCACAG AGGACTTGGA TTATGGCTTT
    TTGTCTAACT ACAAGCTCCT CAACACCGCC ATCACAAGAG CACAGTCCCT GGTTGCCGTG
    GTGGGGGACC CCATCGCTCT GTGCTCCATT GGGAGATGCA GGAAATTTTG GGAGCGGTTT
    ATTGCCCTGT GTCATGAAAA CAATAGCCTG CATGGAATCA CTTTTGAACA GATCAAAGCG
    CAGCTAGAGG CTTTAGAACT AAAGAAGACA TACGTGTTGA ATCCACTGGC ACCTGAATTT
    ATACCCCGGG CTCTAAGAGC ACAGCACGCG GGAAACACCA ACAAACCGCA GCAATCACCC
    CCCAAGGGAA AAAGCCTCCA TCATACCCAG AATGATCATT TCCAGAATGA TGGAATTGTT
    CAGCCCAATC CCTCTGTGCT TATTGGCAAT CCTATCAGAG CATATACTCC TCCACCTCCT
    CTTGGACCTC ACCCAAATTT GGGGAAATCT CCAAGTCCTG TTCAAAGAAT CGATCCTCAC
    ACTGGGACGA GTATTCTTTA TGTACCTGCT GTCTATGGAG GGAATGTCGT TATGTCGGTG
    CCTGTACCTG TGCCATGGAC AGGATACCAG GGCAGGTTTG CGGTTGATCC TCGAATCATT
    ACACACCAGG CAGCAATGGC CTACAACATG AACCTCTTAC AGACACATGG ACGTGGGTCT
    CCTATTCCTT ATGGCCTTGG GCACCACCCA CCTGTCAGCA TAGGGCAGCC ACAGAATCAG
    CATCAGGAGA AGGATCAACA TGAGCAAAGT CGCAACGGTA AAAGTGATAC AAATAATCCT
    GGACCTGAAA TTAATAAGAT TCGAACACCA GAGAAAAAAC CTACAGAAGC AAAACAGATT
    GATTTGGAAT CAAACCCACA GAACAGAAGT CCTGAATCAC GTTCTGGCAT TGTTTATCCC
    CATCCCAAAT TTCATCGCAA AGATAATCTC ACCCCAAGAC ACATAAACCT TCCTCTCCCT
    GCCCCCCATG CCCAGTATGC CATCCCCGGT CGTCATTTTC ATCCCCTCCC CCAGCTCCCG
    AGACCACCCT TCCCAATTCC GCAGCAGCAC GCCTTGCTGA ATCAGCAGCA GAGTAACTTG
    CCTGAACAGC CGAGCCCCGT GCCAGCCCAG CCCAGTCAGG TGGCCCCTCA GCTGGGCCCG
    CCGCCGCCTC AGCTCTCCCC TGCCTACCCG GCTGGCCCCG GCAGCGCGTT TTTCAATAAC
    GCGGTTCCCC ACCGACCACA GTCCCCGCCT GCAGAGGCTG TGGTTCCCGA GCAGCAGCCC
    CCACCCGTGC TGCAGGAGGG CCACAGTCCC CTGCGGGCCC TCGCACAGCC CGGCCCCATC
    CTGCCTTCAC CTCTGAACAG CTTCCCGGAC GAGAGCCCCC CGGGCCTGCC GATGGGGGAG
    GCTTTGGATC GTATGCACGG GAGTGTGGCT CTGGAAACCC TGAGGCAGCA GCATGTGCGG
    CTACAGCAGT GGAGCGAGCA CCACGCCTAT CTCAGTCAGG GCAGCCTCCC GTACCCGCAC
    CATCACCACC CTCACCCACA CCTCCAGCAC CTTCCCCAGC CGCCCGTTGG ATTGCATCAG
    CCCCCAGTGA GAGCAGACTG GAAACTCCCC AGCAGTGCCG AAGATGAAGC AGAAACAACT
    GACTCACGGT TTCAAGACTT ACTCAGAGAA CTGTCTAATC GTGAGCAAAG TGAAACACGG
    GAACTAGCTG AAATGCCACC ACCTCAATCA AGGCTTTTGC AATATAGACA AATTCAGACT
    AGGAGCCCAC CAGCAGCCCC CTCGCCCCCG TCCGGCGCAG ACCACAGCAC CCACTTTCCA
    AACTTCACTG ACAGCAGCAG AGACCTTGAA GTGGCCAACA GCCCGGCGTT CCCACAGCGC
    CTGCCGCCCC CGATGTTTAG CTCTCCTTTC TCGTTGCCAT CTGAGCACCT CACCCCTCCT
    CTGAAATACC TGGCGCCTGA TGGAGCTTGG ACTTTTGCTA ACTTACAGCA GAATCACCTG
    ATGGGGCCGA GTTTCCCCTA CGGCCTACCT CCATTGCCTC ACAGGCCCCC GCAGAACCCT
    TTCGTGCAAA TGCAGACCCA CCAGCCCGCT GTTGGCCAGG AGCCGCTCCA GCCGCTGTCC
    TCTCGGACAG TGTCGTCCTC TTCGCTCCCC AGCTTAGAAG AGTATGAACC CAGAGGACCG
    GGTCGGCCCT TGTACCAAAG AAGAATCTCA TCTAGCTCAG TGCAGCCTTG TTCTGAAGAA
    GCAAGCACTC CTCAAGACAG TCTTGCTCAG CGTAAAGAGC TTCAGGACCA TAATAACCAG
    TCTTCTTTCA CCTTTTCCTC CCCGGAGTCC TGGGTAAACG CCACCTCATC CACCCCCTAT
    CAGAACATTC CGTGCAATGG ATCCAGCAGG CCAGCCCAGC CCAGAGAGTT GGCAGCTCCA
    CCCAAGACCG TCAAAGCCCC CGAGGAACAT CTGAAGCCTG AGAGCCTTGA GGGGTCCAGT
    TCCTTCAATT ACAGCGTGCT GCAGCATCTC GGCCAGTTCC CCCCGCTCAT GCCCAACAAG
    CAGATCGCCG AGTCGGCCAG CAGCAGCAGC CCGCAGAGCT CCGGGGGCGG CAAGCCTGCC
    ATGTCCTACG CCAGCGCCCT GCGGGCCCCT CCAAAGCCCA GGCCCCCGCC CGAGCAGGCC
    AAGAAGAGCA GCGACCCTCT GTCTCTGTTT CAAGAACTCA GCCTGGGGAG CTCAGCCGGC
    AGCAATGGCT TTTACTCCTA TTTTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227077
    Clone ID Related Accession (Same CDS sequence) XM_024981141.1
    Accession Version XM_024981141.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5109bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable helicase with zinc finger domain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAAACCT TGATAGAAAA GTGGATGAAT TCATTATCTC CTGAGAAAGT GCTCAGTGAG 
    TGTGTGGAAG GAGTGAAGGT GGAGCATGAC CCCGGTCTGT CTGTCACCGT CAACACCAAA
    AAGTCCCACC AGACCTGGAC CTTCGCTCTC ACCTGTAAGC CTGCAAGAAT GCTGTGTCGT
    GTAGCATTGC TTTATGATGC TCATCGTCCT CATTTTAGTA TCATTGCAAT ATCTGCCGGA
    GATAGTACTA CCCAGGTATC ACAAGAAGTC CCAGAAAACT GTCAAGAATG GATAGGAGGG
    AAGATGGCCC CAAATGGATT AGATCATTAC GTGTATAAAG TCGGGATAGC ATTTAACACA
    GAAATATTTG GAACTTTTCG CCAAACCATA GTTTTCGACT TTGGATTGGA ACCAGTACTC
    ATGCAAAGAG TAATGATTGA TGCAGCTTCT ACAGAAGATC TTGAGTACCT GATGCATGCA
    AAACAACAGC TAGTAACTAC AGCTAAACGT TGGGATTCTT CTTCTAAGAC TATTGTAGAT
    TTTGAACCTA ATGAAACTAC TGATTTGGAG AAGAGCCTTC TTATCAGATA CCAAATTCCT
    CTCTCTGCTG ACCAGCTGTT TACCCAGTCC GTTTTAGACA AATCATTGAC CAAGACCAAC
    TATCAGTCAC GGTTGCATGA CCTTCTTTAT ATTGAGGAGA TAGCCCAGTA TAAGGAAGTC
    AGCAAGTTCA ATCTTAAAGT GCAGTTGCAG ATTCTAGCAA GCTTCATGCT CACTGGAGTT
    TCTGGAGGTG CAAAGTACGC TCAGAATGGA CAACTTTTTG GTCGCTTTAA GCTTACTGAA
    ACTCTTTCTG AAGATACTTT GGCTGGACGG CTGGTGATGA CCAAAGTCAA TGCTGTTTAT
    TTATTACCAG TCCCTAAAGA AAAGTTAGTA CAGACCCAGG GAACCAAAGA GAAGGTTTAT
    GAAGCTACTA TTGAAGAAAA AACAAAAGAA TATATATTTT TAAGGATATC CAGGGAATGC
    TGTGAAGAAC TTAATCTTCG GCCTGATTAC GACACACAGG TTGAACTCCA GTTTCAGTTA
    AATCGATTAC CCCTCTGTGA AATGCATTAT GCGCTAGACA GGGTCAAGGA CAATGGGGTT
    TTGTTTCCAG ACATCACTAT GGCTCCAACC ATACCTTGGA GTCCCAACAG GCAATGGGAC
    GAACAGTTGG ATCCTCGGCT AAATGCAAAA CAGAAAGAGG CTGTTCTGGC CATTACCACC
    CCACTTTCAA TACAGTTGCC TCCTGTCCTC ATCATCGGCC CATACGGGAC GGGCAAAACA
    TTCACTCTAG CTCAGGCCGT CAAGCACATT CTCCAGCAAC AGGAGACCAG CAGGATTCTC
    ATTTGCACCC ATTCTAATAG TGCTGCTGAT CTCTACATAA AGGATTATTT ACATCCATAT
    GTAGAAGCAG GCAATCCCCA GGCAAGACCT CTCAGGGTAT ATTTCAGAAA CCGCTGGGTG
    AAGACTGTCC ACCCAGTTGT GCATCAGTAC TGTTTGATCT CAAGCGCACA TTCCACCTTT
    CAGATGCCAC AGAAAGAAGA TATTCTGAAA CACCGAGTGG TGGTGGTTAC ATTGAACACT
    TCCCAGTACC TCTGTCAGCT GGACCTCGAA CCTGGGTTTT TTACTCACAT CTTGTTAGAC
    GAGGCTGCCC AGGCCATGGA GTGTGAAACC ATCATGCCTC TGGCACTAGC AACTAGACAC
    ACGCGGGTTG TCCTGGCTGG CGACCACATG CAGCTCAGTC CTTTTGTTTA CAGCGAGTTT
    GCCAGGGAGA GGAACCTTCA CGTGTCGTTA CTTGATCGGC TCTATGAGCA CTACCCTGCC
    GAGTTTCCCT GTAGGATCCT CCTGTGCGAG AACTACCGTT CCCACGAAGC TATCATCAAT
    TACACCTCTG AGCTCTTCTA TGAGGGCAAG CTGATGGCCA GTGGAAAGCA ACCAGCACAC
    AAGGAGTTCT ATCCACTAAC CTTCTTTACA GCACGAGGGG AAGATGTACA GGAGAAAAAT
    AGCACAGCTT TTTATAATAA TGCAGAGGTA TTTGAAGTGG TGGAGCGAGT GGAAGAGCTG
    AGAAGGAAGT GGCCAGTAGC GTGGGGCAAG TTGGATGATG GAAGTATTGG TGTGGTGACT
    CCATATGCAG ACCAGGTGTT CAGGATACGC GCCGAACTTC GAAAAAAGAG GTTATCTGAC
    GTTAACGTAG AAAGGGTGCT CAATGTTCAA GGAAAGCAAT TCAGAGTTTT GTTTCTTAGC
    ACAGTACGTA CAAGGCATAC TTGTAAACAT AAACAGACAC CAATTAAAAA GAAGGAGCAG
    CTGCTAGAAG ACTCCACAGA GGACTTGGAT TATGGCTTTT TGTCTAACTA CAAGCTCCTC
    AACACCGCCA TCACAAGAGC ACAGTCCCTG GTTGCCGTGG TGGGGGACCC CATCGCTCTG
    TGCTCCATTG GGAGATGCAG GAAATTTTGG GAGCGGTTTA TTGCCCTGTG TCATGAAAAC
    AATAGCCTGC ATGGAATCAC TTTTGAACAG ATCAAAGCGC AGCTAGAGGC TTTAGAACTA
    AAGAAGACAT ACGTGTTGAA TCCACTGGCA CCTGAATTTA TACCCCGGGC TCTAAGAGCA
    CAGCACGCGG GAAACACCAA CAAACCGCAG CAATCACCCC CCAAGGGAAA AAGCCTCCAT
    CATACCCAGA ATGATCATTT CCAGAATGAT GGAATTGTTC AGCCCAATCC CTCTGTGCTT
    ATTGGCAATC CTATCAGAGC ATATACTCCT CCACCTCCTC TTGGACCTCA CCCAAATTTG
    GGGAAATCTC CAAGTCCTGT TCAAAGAATC GATCCTCACA CTGGGACGAG TATTCTTTAT
    GTACCTGCTG TCTATGGAGG GAATGTCGTT ATGTCGGTGC CTGTACCTGT GCCATGGACA
    GGATACCAGG GCAGGTTTGC GGTTGATCCT CGAATCATTA CACACCAGGC AGCAATGGCC
    TACAACATGA ACCTCTTACA GACACATGGA CGTGGGTCTC CTATTCCTTA TGGCCTTGGG
    CACCACCCAC CTGTCAGCAT AGGGCAGCCA CAGAATCAGC ATCAGGAGAA GGATCAACAT
    GAGCAAAGTC GCAACGGTAA AAGTGATACA AATAATCCTG GACCTGAAAT TAATAAGATT
    CGAACACCAG AGAAAAAACC TACAGAAGCA AAACAGATTG ATTTGGAATC AAACCCACAG
    AACAGAAGTC CTGAATCACG TTCTGGCATT GTTTATCCCC ATCCCAAATT TCATCGCAAA
    GATAATCTCA CCCCAAGACA CATAAACCTT CCTCTCCCTG CCCCCCATGC CCAGTATGCC
    ATCCCCGGTC GTCATTTTCA TCCCCTCCCC CAGCTCCCGA GACCACCCTT CCCAATTCCG
    CAGCAGCACG CCTTGCTGAA TCAGCAGCAG AGTAACTTGC CTGAACAGCC GAGCCCCGTG
    CCAGCCCAGC CCAGTCAGGT GGCCCCTCAG CTGGGCCCGC CGCCGCCTCA GCTCTCCCCT
    GCCTACCCGG CTGGCCCCGG CAGCGCGTTT TTCAATAACG CGGTTCCCCA CCGACCACAG
    TCCCCGCCTG CAGAGGCTGT GGTTCCCGAG CAGCAGCCCC CACCCGTGCT GCAGGAGGGC
    CACAGTCCCC TGCGGGCCCT CGCACAGCCC GGCCCCATCC TGCCTTCACC TCTGAACAGC
    TTCCCGGACG AGAGCCCCCC GGGCCTGCCG ATGGGGGAGG CTTTGGATCG TATGCACGGG
    AGTGTGGCTC TGGAAACCCT GAGGCAGCAG CATGTGCGGC TACAGCAGTG GAGCGAGCAC
    CACGCCTATC TCAGTCAGGG CAGCCTCCCG TACCCGCACC ATCACCACCC TCACCCACAC
    CTCCAGCACC TTCCCCAGCC GCCCGTTGGA TTGCATCAGC CCCCAGTGAG AGCAGACTGG
    AAACTCCCCA GCAGTGCCGA AGATGAAGCA GAAACAACTG ACTCACGGTT TCAAGACTTA
    CTCAGAGAAC TGTCTAATCG TGAGCAAAGT GAAACACGGG AACTAGCTGA AATGCCACCA
    CCTCAATCAA GGCTTTTGCA ATATAGACAA ATTCAGACTA GGAGCCCACC AGCAGCCCCC
    TCGCCCCCGT CCGGCGCAGA CCACAGCACC CACTTTCCAA ACTTCACTGA CAGCAGCAGA
    GACCTTGAAG TGGCCAACAG CCCGGCGTTC CCACAGCGCC TGCCGCCCCC GATGTTTAGC
    TCTCCTTTCT CGTTGCCATC TGAGCACCTC ACCCCTCCTC TGAAATACCT GGCGCCTGAT
    GGAGCTTGGA CTTTTGCTAA CTTACAGCAG AATCACCTGA TGGGGCCGAG TTTCCCCTAC
    GGCCTACCTC CATTGCCTCA CAGGCCCCCG CAGAACCCTT TCGTGCAAAT GCAGACCCAC
    CAGCCCGCTG TTGGCCAGGA GCCGCTCCAG CCGCTGTCCT CTCGGACAGT GTCGTCCTCT
    TCGCTCCCCA GCTTAGAAGA GTATGAACCC AGAGGACCGG GTCGGCCCTT GTACCAAAGA
    AGAATCTCAT CTAGCTCAGT GCAGCCTTGT TCTGAAGAAG CAAGCACTCC TCAAGACAGT
    CTTGCTCAGC GTAAAGAGCT TCAGGACCAT AATAACCAGT CTTCTTTCAC CTTTTCCTCC
    CCGGAGTCCT GGGTAAACGC CACCTCATCC ACCCCCTATC AGAACATTCC GTGCAATGGA
    TCCAGCAGGC CAGCCCAGCC CAGAGAGTTG GCAGCTCCAC CCAAGACCGT CAAAGCCCCC
    GAGGAACATC TGAAGCCTGA GAGCCTTGAG GGGTCCAGTT CCTTCAATTA CAGCGTGCTG
    CAGCATCTCG GCCAGTTCCC CCCGCTCATG CCCAACAAGC AGATCGCCGA GTCGGCCAGC
    AGCAGCAGCC CGCAGAGCTC CGGGGGCGGC AAGCCTGCCA TGTCCTACGC CAGCGCCCTG
    CGGGCCCCTC CAAAGCCCAG GCCCCCGCCC GAGCAGGCCA AGAAGAGCAG CGACCCTCTG
    TCTCTGTTTC AAGAACTCAG CCTGGGGAGC TCAGCCGGCA GCAATGGCTT TTACTCCTAT
    TTTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836909.1
    CDS264..5372
    Translation

    Target ORF information:

    RefSeq Version XM_024981141.1
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase with zinc finger (HELZ), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981141.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAAACCT TGATAGAAAA GTGGATGAAT TCATTATCTC CTGAGAAAGT GCTCAGTGAG 
    TGTGTGGAAG GAGTGAAGGT GGAGCATGAC CCCGGTCTGT CTGTCACCGT CAACACCAAA
    AAGTCCCACC AGACCTGGAC CTTCGCTCTC ACCTGTAAGC CTGCAAGAAT GCTGTGTCGT
    GTAGCATTGC TTTATGATGC TCATCGTCCT CATTTTAGTA TCATTGCAAT ATCTGCCGGA
    GATAGTACTA CCCAGGTATC ACAAGAAGTC CCAGAAAACT GTCAAGAATG GATAGGAGGG
    AAGATGGCCC CAAATGGATT AGATCATTAC GTGTATAAAG TCGGGATAGC ATTTAACACA
    GAAATATTTG GAACTTTTCG CCAAACCATA GTTTTCGACT TTGGATTGGA ACCAGTACTC
    ATGCAAAGAG TAATGATTGA TGCAGCTTCT ACAGAAGATC TTGAGTACCT GATGCATGCA
    AAACAACAGC TAGTAACTAC AGCTAAACGT TGGGATTCTT CTTCTAAGAC TATTGTAGAT
    TTTGAACCTA ATGAAACTAC TGATTTGGAG AAGAGCCTTC TTATCAGATA CCAAATTCCT
    CTCTCTGCTG ACCAGCTGTT TACCCAGTCC GTTTTAGACA AATCATTGAC CAAGACCAAC
    TATCAGTCAC GGTTGCATGA CCTTCTTTAT ATTGAGGAGA TAGCCCAGTA TAAGGAAGTC
    AGCAAGTTCA ATCTTAAAGT GCAGTTGCAG ATTCTAGCAA GCTTCATGCT CACTGGAGTT
    TCTGGAGGTG CAAAGTACGC TCAGAATGGA CAACTTTTTG GTCGCTTTAA GCTTACTGAA
    ACTCTTTCTG AAGATACTTT GGCTGGACGG CTGGTGATGA CCAAAGTCAA TGCTGTTTAT
    TTATTACCAG TCCCTAAAGA AAAGTTAGTA CAGACCCAGG GAACCAAAGA GAAGGTTTAT
    GAAGCTACTA TTGAAGAAAA AACAAAAGAA TATATATTTT TAAGGATATC CAGGGAATGC
    TGTGAAGAAC TTAATCTTCG GCCTGATTAC GACACACAGG TTGAACTCCA GTTTCAGTTA
    AATCGATTAC CCCTCTGTGA AATGCATTAT GCGCTAGACA GGGTCAAGGA CAATGGGGTT
    TTGTTTCCAG ACATCACTAT GGCTCCAACC ATACCTTGGA GTCCCAACAG GCAATGGGAC
    GAACAGTTGG ATCCTCGGCT AAATGCAAAA CAGAAAGAGG CTGTTCTGGC CATTACCACC
    CCACTTTCAA TACAGTTGCC TCCTGTCCTC ATCATCGGCC CATACGGGAC GGGCAAAACA
    TTCACTCTAG CTCAGGCCGT CAAGCACATT CTCCAGCAAC AGGAGACCAG CAGGATTCTC
    ATTTGCACCC ATTCTAATAG TGCTGCTGAT CTCTACATAA AGGATTATTT ACATCCATAT
    GTAGAAGCAG GCAATCCCCA GGCAAGACCT CTCAGGGTAT ATTTCAGAAA CCGCTGGGTG
    AAGACTGTCC ACCCAGTTGT GCATCAGTAC TGTTTGATCT CAAGCGCACA TTCCACCTTT
    CAGATGCCAC AGAAAGAAGA TATTCTGAAA CACCGAGTGG TGGTGGTTAC ATTGAACACT
    TCCCAGTACC TCTGTCAGCT GGACCTCGAA CCTGGGTTTT TTACTCACAT CTTGTTAGAC
    GAGGCTGCCC AGGCCATGGA GTGTGAAACC ATCATGCCTC TGGCACTAGC AACTAGACAC
    ACGCGGGTTG TCCTGGCTGG CGACCACATG CAGCTCAGTC CTTTTGTTTA CAGCGAGTTT
    GCCAGGGAGA GGAACCTTCA CGTGTCGTTA CTTGATCGGC TCTATGAGCA CTACCCTGCC
    GAGTTTCCCT GTAGGATCCT CCTGTGCGAG AACTACCGTT CCCACGAAGC TATCATCAAT
    TACACCTCTG AGCTCTTCTA TGAGGGCAAG CTGATGGCCA GTGGAAAGCA ACCAGCACAC
    AAGGAGTTCT ATCCACTAAC CTTCTTTACA GCACGAGGGG AAGATGTACA GGAGAAAAAT
    AGCACAGCTT TTTATAATAA TGCAGAGGTA TTTGAAGTGG TGGAGCGAGT GGAAGAGCTG
    AGAAGGAAGT GGCCAGTAGC GTGGGGCAAG TTGGATGATG GAAGTATTGG TGTGGTGACT
    CCATATGCAG ACCAGGTGTT CAGGATACGC GCCGAACTTC GAAAAAAGAG GTTATCTGAC
    GTTAACGTAG AAAGGGTGCT CAATGTTCAA GGAAAGCAAT TCAGAGTTTT GTTTCTTAGC
    ACAGTACGTA CAAGGCATAC TTGTAAACAT AAACAGACAC CAATTAAAAA GAAGGAGCAG
    CTGCTAGAAG ACTCCACAGA GGACTTGGAT TATGGCTTTT TGTCTAACTA CAAGCTCCTC
    AACACCGCCA TCACAAGAGC ACAGTCCCTG GTTGCCGTGG TGGGGGACCC CATCGCTCTG
    TGCTCCATTG GGAGATGCAG GAAATTTTGG GAGCGGTTTA TTGCCCTGTG TCATGAAAAC
    AATAGCCTGC ATGGAATCAC TTTTGAACAG ATCAAAGCGC AGCTAGAGGC TTTAGAACTA
    AAGAAGACAT ACGTGTTGAA TCCACTGGCA CCTGAATTTA TACCCCGGGC TCTAAGAGCA
    CAGCACGCGG GAAACACCAA CAAACCGCAG CAATCACCCC CCAAGGGAAA AAGCCTCCAT
    CATACCCAGA ATGATCATTT CCAGAATGAT GGAATTGTTC AGCCCAATCC CTCTGTGCTT
    ATTGGCAATC CTATCAGAGC ATATACTCCT CCACCTCCTC TTGGACCTCA CCCAAATTTG
    GGGAAATCTC CAAGTCCTGT TCAAAGAATC GATCCTCACA CTGGGACGAG TATTCTTTAT
    GTACCTGCTG TCTATGGAGG GAATGTCGTT ATGTCGGTGC CTGTACCTGT GCCATGGACA
    GGATACCAGG GCAGGTTTGC GGTTGATCCT CGAATCATTA CACACCAGGC AGCAATGGCC
    TACAACATGA ACCTCTTACA GACACATGGA CGTGGGTCTC CTATTCCTTA TGGCCTTGGG
    CACCACCCAC CTGTCAGCAT AGGGCAGCCA CAGAATCAGC ATCAGGAGAA GGATCAACAT
    GAGCAAAGTC GCAACGGTAA AAGTGATACA AATAATCCTG GACCTGAAAT TAATAAGATT
    CGAACACCAG AGAAAAAACC TACAGAAGCA AAACAGATTG ATTTGGAATC AAACCCACAG
    AACAGAAGTC CTGAATCACG TTCTGGCATT GTTTATCCCC ATCCCAAATT TCATCGCAAA
    GATAATCTCA CCCCAAGACA CATAAACCTT CCTCTCCCTG CCCCCCATGC CCAGTATGCC
    ATCCCCGGTC GTCATTTTCA TCCCCTCCCC CAGCTCCCGA GACCACCCTT CCCAATTCCG
    CAGCAGCACG CCTTGCTGAA TCAGCAGCAG AGTAACTTGC CTGAACAGCC GAGCCCCGTG
    CCAGCCCAGC CCAGTCAGGT GGCCCCTCAG CTGGGCCCGC CGCCGCCTCA GCTCTCCCCT
    GCCTACCCGG CTGGCCCCGG CAGCGCGTTT TTCAATAACG CGGTTCCCCA CCGACCACAG
    TCCCCGCCTG CAGAGGCTGT GGTTCCCGAG CAGCAGCCCC CACCCGTGCT GCAGGAGGGC
    CACAGTCCCC TGCGGGCCCT CGCACAGCCC GGCCCCATCC TGCCTTCACC TCTGAACAGC
    TTCCCGGACG AGAGCCCCCC GGGCCTGCCG ATGGGGGAGG CTTTGGATCG TATGCACGGG
    AGTGTGGCTC TGGAAACCCT GAGGCAGCAG CATGTGCGGC TACAGCAGTG GAGCGAGCAC
    CACGCCTATC TCAGTCAGGG CAGCCTCCCG TACCCGCACC ATCACCACCC TCACCCACAC
    CTCCAGCACC TTCCCCAGCC GCCCGTTGGA TTGCATCAGC CCCCAGTGAG AGCAGACTGG
    AAACTCCCCA GCAGTGCCGA AGATGAAGCA GAAACAACTG ACTCACGGTT TCAAGACTTA
    CTCAGAGAAC TGTCTAATCG TGAGCAAAGT GAAACACGGG AACTAGCTGA AATGCCACCA
    CCTCAATCAA GGCTTTTGCA ATATAGACAA ATTCAGACTA GGAGCCCACC AGCAGCCCCC
    TCGCCCCCGT CCGGCGCAGA CCACAGCACC CACTTTCCAA ACTTCACTGA CAGCAGCAGA
    GACCTTGAAG TGGCCAACAG CCCGGCGTTC CCACAGCGCC TGCCGCCCCC GATGTTTAGC
    TCTCCTTTCT CGTTGCCATC TGAGCACCTC ACCCCTCCTC TGAAATACCT GGCGCCTGAT
    GGAGCTTGGA CTTTTGCTAA CTTACAGCAG AATCACCTGA TGGGGCCGAG TTTCCCCTAC
    GGCCTACCTC CATTGCCTCA CAGGCCCCCG CAGAACCCTT TCGTGCAAAT GCAGACCCAC
    CAGCCCGCTG TTGGCCAGGA GCCGCTCCAG CCGCTGTCCT CTCGGACAGT GTCGTCCTCT
    TCGCTCCCCA GCTTAGAAGA GTATGAACCC AGAGGACCGG GTCGGCCCTT GTACCAAAGA
    AGAATCTCAT CTAGCTCAGT GCAGCCTTGT TCTGAAGAAG CAAGCACTCC TCAAGACAGT
    CTTGCTCAGC GTAAAGAGCT TCAGGACCAT AATAACCAGT CTTCTTTCAC CTTTTCCTCC
    CCGGAGTCCT GGGTAAACGC CACCTCATCC ACCCCCTATC AGAACATTCC GTGCAATGGA
    TCCAGCAGGC CAGCCCAGCC CAGAGAGTTG GCAGCTCCAC CCAAGACCGT CAAAGCCCCC
    GAGGAACATC TGAAGCCTGA GAGCCTTGAG GGGTCCAGTT CCTTCAATTA CAGCGTGCTG
    CAGCATCTCG GCCAGTTCCC CCCGCTCATG CCCAACAAGC AGATCGCCGA GTCGGCCAGC
    AGCAGCAGCC CGCAGAGCTC CGGGGGCGGC AAGCCTGCCA TGTCCTACGC CAGCGCCCTG
    CGGGCCCCTC CAAAGCCCAG GCCCCCGCCC GAGCAGGCCA AGAAGAGCAG CGACCCTCTG
    TCTCTGTTTC AAGAACTCAG CCTGGGGAGC TCAGCCGGCA GCAATGGCTT TTACTCCTAT
    TTTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94083
    Clone ID Related Accession (Same CDS sequence) XM_015459037.1
    Accession Version XM_015459037.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4998bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable helicase with zinc finger domain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104502.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGGTAATATT CTCCCCCCTG CGCCTCTCCA TTGCCCGGTC
    TCCCCCCTGC GCCTCTCCAT TGCCCGGTCT CCCCCCTGCG CCTCTCCATT GCCCGGTCTC
    CCCGCTGCGC CTCTCCGTTG CCTGGTCTCC CCCCTGCGCC TCTCCGTTGC CCAGTCTCCC
    CTCTGCGCCT CTCGATTGCC CGGACATGTT ATCTTCTTTT ATAGGGTATA TTTCAGAAAC
    CGCTGGGTGA AGACTGTCCA CCCAGTTGTG CATCAGTACT GTTTGATCTC AAGCGCACAT
    TCCACCTTTC AGATGCCACA GAAAGAAGAT ATTCTGAAAC ACCGAGTGGT GGTGGTTACA
    TTGAACACTT CCCAGTACCT CTGTCAGCTG GACCTCGAAC CTGGGTTTTT TACTCACATC
    TTGTTAGACG AGGCTGCCCA GGCCATGGAG TGTGAAACCA TCATGCCTCT GGCACTAGCA
    ACTAGACACA CGCGGGTTGT CCTGGCTGGC GACCACATGC AGCTCAGTCC TTTTGTTTAC
    AGCGAGTTTG CCAGGGAGAG GAACCTTCAC GTGTCGTTAC TTGATCGGCT CTATGAGCAC
    TACCCTGCCG AGTTTCCCTG TAGGATCCTC CTGTGCGAGA ACTACCGTTC CCACGAAGCT
    ATCATCAATT ACACCTCTGA GCTCTTCTAT GAGGGCAAGC TGATGGCCAG TGGAAAGCAA
    CCAGCACACA AGGAGTTCTA TCCACTAACC TTCTTTACAG CACGAGGGGA AGATGTACAG
    GAGAAAAATA GCACAGCTTT TTATAATAAT GCAGAGGTAT TTGAAGTGGT GGAGCGAGTG
    GAAGAGCTGA GAAGGAAGTG GCCAGTAGCG TGGGGCAAGT TGGATGATGG AAGTATTGGT
    GTGGTGACTC CATATGCAGA CCAGGTGTTC AGGATACGCG CCGAACTTCG AAAAAAGAGG
    TTATCTGACG TTAACGTAGA AAGGGTGCTC AATGTTCAAG GAAAGCAATT CAGAGTTTTG
    TTTCTTAGCA CAGTACGTAC AAGGCATACT TGTAAACATA AACAGACACC AATTAAAAAG
    AAGGAGCAGC TGCTAGAAGA CTCCACAGAG GACTTGGATT ATGGCTTTTT GTCTAACTAC
    AAGCTCCTCA ACACCGCCAT CACAAGAGCA CAGTCCCTGG TTGCCGTGGT GGGGGACCCC
    ATCGCTCTGT GCTCCATTGG GAGATGCAGG AAATTTTGGG AGCGGTTTAT TGCCCTGTGT
    CATGAAAACA ATAGCCTGCA TGGAATCACT TTTGAACAGA TCAAAGCGCA GCTAGAGGCT
    TTAGAACTAA AGAAGACATA CGTGTTGAAT CCACTGGCAC CTGAATTTAT ACCCCGGGCT
    CTAAGAGCAC AGCACGCGGG AAACACCAAC AAACCGCAGC AATCACCCCC CAAGGGAAAA
    AGCCTCCATC ATACCCAGAA TGATCATTTC CAGAATGATG GAATTGTTCA GCCCAATCCC
    TCTGTGCTTA TTGGCAATCC TATCAGAGCA TATACTCCTC CACCTCCTCT TGGACCTCAC
    CCAAATTTGG GGAAATCTCC AAGTCCTGTT CAAAGAATCG ATCCTCACAC TGGGACGAGT
    ATTCTTTATG TACCTGCTGT CTATGGAGGG AATGTCGTTA TGTCGGTGCC TGTACCTGTG
    CCATGGACAG GATACCAGGG CAGGTTTGCG GTTGATCCTC GAATCATTAC ACACCAGGCA
    GCAATGGCCT ACAACATGAA CCTCTTACAG ACACATGGAC GTGGGTCTCC TATTCCTTAT
    GGCCTTGGGC ACCACCCACC TGTCAGCATA GGGCAGCCAC AGAATCAGCA TCAGGAGAAG
    GATCAACATG AGCAAAGTCG CAACGGTAAA AGTGATACAA ATAATCCTGG ACCTGAAATT
    AATAAGATTC GAACACCAGA GAAAAAACCT ACAGAAGCAA AACAGATTGA TTTGGAATCA
    AACCCACAGA ACAGAAGTCC TGAATCACGT TCTGGCATTG TTTATCCCCA TCCCAAATTT
    CATCGCAAAG ATAATCTCAC CCCAAGACAC ATAAACCTTC CTCTCCCTGC CCCCCATGCC
    CAGTATGCCA TCCCCGGTCG TCATTTTCAT CCCCTCCCCC AGCTCCCGAG ACCACCCTTC
    CCAATTCCGC AGCAGCACGC CTTGCTGAAT CAGCAGCAGA GTAACTTGCC TGAACAGCCG
    AGCCCCGTGC CAGCCCAGCC CAGTCAGGTG GCCCCTCAGC TGGGCCCGCC GCCGCCTCAG
    CTCTCCCCTG CCTACCCGGC TGGCCCCGGC AGCGCGTTTT TCAATAACGC GGTTCCCCAC
    CGACCACAGT CCCCGCCTGC AGAGGCTGTG GTTCCCGAGC AGCAGCCCCC ACCCGTGCTG
    CAGGAGGGCC ACAGTCCCCT GCGGGCCCTC GCACAGCCCG GCCCCATCCT GCCTTCACCT
    CTGAACAGCT TCCCGGACGA GAGCCCCCCG GGCCTGCCGA TGGGGGAGGC TTTGGATCGT
    ATGCACGGGA GTGTGGCTCT GGAAACCCTG AGGCAGCAGC ATGTGCGGCT ACAGCAGTGG
    AGCGAGCACC ACGCCTATCT CAGTCAGGGC AGCCTCCCGT ACCCGCACCA TCACCACCCT
    CACCCACACC TCCAGCACCT TCCCCAGCCG CCCGTTGGAT TGCATCAGCC CCCAGTGAGA
    GCAGACTGGA AACTCCCCAG CAGTGCCGAA GATGAAGCAG AAACAACTGA CTCACGGTTT
    CAAGACTTAC TCAGAGAACT GTCTAATCGT GAGCAAAGTG AAACACGGGA ACTAGCTGAA
    ATGCCACCAC CTCAATCAAG GCTTTTGCAA TATAGACAAA TTCAGACTAG GAGCCCACCA
    GCAGCCCCCT CGCCCCCGTC CGGCGCAGAC CACAGCACCC ACTTTCCAAA CTTCACTGAC
    AGCAGCAGAG ACCTTGAAGT GGCCAACAGC CCGGCGTTCC CACAGCGCCT GCCGCCCCCG
    ATGTTTAGCT CTCCTTTCTC GTTGCCATCT GAGCACCTCA CCCCTCCTCT GAAATACCTG
    GCGCCTGATG GAGCTTGGAC TTTTGCTAAC TTACAGCAGA ATCACCTGAT GGGGCCGAGT
    TTCCCCTACG GCCTACCTCC ATTGCCTCAC AGGCCCCCGC AGAACCCTTT CGTGCAAATG
    CAGACCCACC AGCCCGCTGT TGGCCAGGAG CCGCTCCAGC CGCTGTCCTC TCGGACAGTG
    TCGTCCTCTT CGCTCCCCAG CTTAGAAGAG TATGAACCCA GAGGACCGGG TCGGCCCTTG
    TACCAAAGAA GAATCTCATC TAGCTCAGTG CAGCCTTGTT CTGAAGAAGC AAGCACTCCT
    CAAGACAGTC TTGCTCAGCG TAAAGAGCTT CAGGACCATA ATAACCAGTC TTCTTTCACC
    TTTTCCTCCC CGGAGTCCTG GGTAAACGCC ACCTCATCCA CCCCCTATCA GAACATTCCG
    TGCAATGGAT CCAGCAGGCC AGCCCAGCCC AGAGAGTTGG CAGCTCCACC CAAGACCGTC
    AAAGCCCCCG AGGAACATCT GAAGCCTGAG AGCCTTGAGG GGTCCAGTTC CTTCAATTAC
    AGCGTGCTGC AGCATCTCGG CCAGTTCCCC CCGCTCATGC CCAACAAGCA GATCGCCGAG
    TCGGCCAGCA GCAGCAGCCC GCAGAGCTCC GGGGGCGGCA AGCCTGCCAT GTCCTACGCC
    AGCGCCCTGC GGGCCCCTCC AAAGCCCAGG CCCCCGCCCG AGCAGGCCAA GAAGAGCAGC
    GACCCTCTGT CTCTGTTTCA AGAACTCAGC CTGGGGAGCT CAGCCGGCAG CAATGGCTTT
    TACTCCTATT TTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314523.1
    CDS219..5216
    Translation

    Target ORF information:

    RefSeq Version XM_015459037.1
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase with zinc finger (HELZ), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459037.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGGTAATATT CTCCCCCCTG CGCCTCTCCA TTGCCCGGTC
    TCCCCCCTGC GCCTCTCCAT TGCCCGGTCT CCCCCCTGCG CCTCTCCATT GCCCGGTCTC
    CCCGCTGCGC CTCTCCGTTG CCTGGTCTCC CCCCTGCGCC TCTCCGTTGC CCAGTCTCCC
    CTCTGCGCCT CTCGATTGCC CGGACATGTT ATCTTCTTTT ATAGGGTATA TTTCAGAAAC
    CGCTGGGTGA AGACTGTCCA CCCAGTTGTG CATCAGTACT GTTTGATCTC AAGCGCACAT
    TCCACCTTTC AGATGCCACA GAAAGAAGAT ATTCTGAAAC ACCGAGTGGT GGTGGTTACA
    TTGAACACTT CCCAGTACCT CTGTCAGCTG GACCTCGAAC CTGGGTTTTT TACTCACATC
    TTGTTAGACG AGGCTGCCCA GGCCATGGAG TGTGAAACCA TCATGCCTCT GGCACTAGCA
    ACTAGACACA CGCGGGTTGT CCTGGCTGGC GACCACATGC AGCTCAGTCC TTTTGTTTAC
    AGCGAGTTTG CCAGGGAGAG GAACCTTCAC GTGTCGTTAC TTGATCGGCT CTATGAGCAC
    TACCCTGCCG AGTTTCCCTG TAGGATCCTC CTGTGCGAGA ACTACCGTTC CCACGAAGCT
    ATCATCAATT ACACCTCTGA GCTCTTCTAT GAGGGCAAGC TGATGGCCAG TGGAAAGCAA
    CCAGCACACA AGGAGTTCTA TCCACTAACC TTCTTTACAG CACGAGGGGA AGATGTACAG
    GAGAAAAATA GCACAGCTTT TTATAATAAT GCAGAGGTAT TTGAAGTGGT GGAGCGAGTG
    GAAGAGCTGA GAAGGAAGTG GCCAGTAGCG TGGGGCAAGT TGGATGATGG AAGTATTGGT
    GTGGTGACTC CATATGCAGA CCAGGTGTTC AGGATACGCG CCGAACTTCG AAAAAAGAGG
    TTATCTGACG TTAACGTAGA AAGGGTGCTC AATGTTCAAG GAAAGCAATT CAGAGTTTTG
    TTTCTTAGCA CAGTACGTAC AAGGCATACT TGTAAACATA AACAGACACC AATTAAAAAG
    AAGGAGCAGC TGCTAGAAGA CTCCACAGAG GACTTGGATT ATGGCTTTTT GTCTAACTAC
    AAGCTCCTCA ACACCGCCAT CACAAGAGCA CAGTCCCTGG TTGCCGTGGT GGGGGACCCC
    ATCGCTCTGT GCTCCATTGG GAGATGCAGG AAATTTTGGG AGCGGTTTAT TGCCCTGTGT
    CATGAAAACA ATAGCCTGCA TGGAATCACT TTTGAACAGA TCAAAGCGCA GCTAGAGGCT
    TTAGAACTAA AGAAGACATA CGTGTTGAAT CCACTGGCAC CTGAATTTAT ACCCCGGGCT
    CTAAGAGCAC AGCACGCGGG AAACACCAAC AAACCGCAGC AATCACCCCC CAAGGGAAAA
    AGCCTCCATC ATACCCAGAA TGATCATTTC CAGAATGATG GAATTGTTCA GCCCAATCCC
    TCTGTGCTTA TTGGCAATCC TATCAGAGCA TATACTCCTC CACCTCCTCT TGGACCTCAC
    CCAAATTTGG GGAAATCTCC AAGTCCTGTT CAAAGAATCG ATCCTCACAC TGGGACGAGT
    ATTCTTTATG TACCTGCTGT CTATGGAGGG AATGTCGTTA TGTCGGTGCC TGTACCTGTG
    CCATGGACAG GATACCAGGG CAGGTTTGCG GTTGATCCTC GAATCATTAC ACACCAGGCA
    GCAATGGCCT ACAACATGAA CCTCTTACAG ACACATGGAC GTGGGTCTCC TATTCCTTAT
    GGCCTTGGGC ACCACCCACC TGTCAGCATA GGGCAGCCAC AGAATCAGCA TCAGGAGAAG
    GATCAACATG AGCAAAGTCG CAACGGTAAA AGTGATACAA ATAATCCTGG ACCTGAAATT
    AATAAGATTC GAACACCAGA GAAAAAACCT ACAGAAGCAA AACAGATTGA TTTGGAATCA
    AACCCACAGA ACAGAAGTCC TGAATCACGT TCTGGCATTG TTTATCCCCA TCCCAAATTT
    CATCGCAAAG ATAATCTCAC CCCAAGACAC ATAAACCTTC CTCTCCCTGC CCCCCATGCC
    CAGTATGCCA TCCCCGGTCG TCATTTTCAT CCCCTCCCCC AGCTCCCGAG ACCACCCTTC
    CCAATTCCGC AGCAGCACGC CTTGCTGAAT CAGCAGCAGA GTAACTTGCC TGAACAGCCG
    AGCCCCGTGC CAGCCCAGCC CAGTCAGGTG GCCCCTCAGC TGGGCCCGCC GCCGCCTCAG
    CTCTCCCCTG CCTACCCGGC TGGCCCCGGC AGCGCGTTTT TCAATAACGC GGTTCCCCAC
    CGACCACAGT CCCCGCCTGC AGAGGCTGTG GTTCCCGAGC AGCAGCCCCC ACCCGTGCTG
    CAGGAGGGCC ACAGTCCCCT GCGGGCCCTC GCACAGCCCG GCCCCATCCT GCCTTCACCT
    CTGAACAGCT TCCCGGACGA GAGCCCCCCG GGCCTGCCGA TGGGGGAGGC TTTGGATCGT
    ATGCACGGGA GTGTGGCTCT GGAAACCCTG AGGCAGCAGC ATGTGCGGCT ACAGCAGTGG
    AGCGAGCACC ACGCCTATCT CAGTCAGGGC AGCCTCCCGT ACCCGCACCA TCACCACCCT
    CACCCACACC TCCAGCACCT TCCCCAGCCG CCCGTTGGAT TGCATCAGCC CCCAGTGAGA
    GCAGACTGGA AACTCCCCAG CAGTGCCGAA GATGAAGCAG AAACAACTGA CTCACGGTTT
    CAAGACTTAC TCAGAGAACT GTCTAATCGT GAGCAAAGTG AAACACGGGA ACTAGCTGAA
    ATGCCACCAC CTCAATCAAG GCTTTTGCAA TATAGACAAA TTCAGACTAG GAGCCCACCA
    GCAGCCCCCT CGCCCCCGTC CGGCGCAGAC CACAGCACCC ACTTTCCAAA CTTCACTGAC
    AGCAGCAGAG ACCTTGAAGT GGCCAACAGC CCGGCGTTCC CACAGCGCCT GCCGCCCCCG
    ATGTTTAGCT CTCCTTTCTC GTTGCCATCT GAGCACCTCA CCCCTCCTCT GAAATACCTG
    GCGCCTGATG GAGCTTGGAC TTTTGCTAAC TTACAGCAGA ATCACCTGAT GGGGCCGAGT
    TTCCCCTACG GCCTACCTCC ATTGCCTCAC AGGCCCCCGC AGAACCCTTT CGTGCAAATG
    CAGACCCACC AGCCCGCTGT TGGCCAGGAG CCGCTCCAGC CGCTGTCCTC TCGGACAGTG
    TCGTCCTCTT CGCTCCCCAG CTTAGAAGAG TATGAACCCA GAGGACCGGG TCGGCCCTTG
    TACCAAAGAA GAATCTCATC TAGCTCAGTG CAGCCTTGTT CTGAAGAAGC AAGCACTCCT
    CAAGACAGTC TTGCTCAGCG TAAAGAGCTT CAGGACCATA ATAACCAGTC TTCTTTCACC
    TTTTCCTCCC CGGAGTCCTG GGTAAACGCC ACCTCATCCA CCCCCTATCA GAACATTCCG
    TGCAATGGAT CCAGCAGGCC AGCCCAGCCC AGAGAGTTGG CAGCTCCACC CAAGACCGTC
    AAAGCCCCCG AGGAACATCT GAAGCCTGAG AGCCTTGAGG GGTCCAGTTC CTTCAATTAC
    AGCGTGCTGC AGCATCTCGG CCAGTTCCCC CCGCTCATGC CCAACAAGCA GATCGCCGAG
    TCGGCCAGCA GCAGCAGCCC GCAGAGCTCC GGGGGCGGCA AGCCTGCCAT GTCCTACGCC
    AGCGCCCTGC GGGCCCCTCC AAAGCCCAGG CCCCCGCCCG AGCAGGCCAA GAAGAGCAGC
    GACCCTCTGT CTCTGTTTCA AGAACTCAGC CTGGGGAGCT CAGCCGGCAG CAATGGCTTT
    TACTCCTATT TTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa95076
    Clone ID Related Accession (Same CDS sequence) XM_015468293.1
    Accession Version XM_015468293.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable helicase with zinc finger domain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646471.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAATCT CCCCCCTGCG
    CCTCTCCATT GCCCGGTCTC CCCCCTGCGC CTCTCCATTG CCCGGTCTCC CCCCTGCGCC
    TCTCCATTGC CCGGTCTCCC CGCTGCGCCT CTCCGTTGCC TGGTCTCCCC CCTGCGCCTC
    TCCGTTGCCC AGTCTCCCCT CTGCGCCTCT CGATTGCCCG GACATGTTAT CTTCTTTTAT
    AGGGTATATT TCAGAAACCG CTGGGTGAAG ACTGTCCACC CAGTTGTGCA TCAGTACTGT
    TTGATCTCAA GCGCACATTC CACCTTTCAG ATGCCACAGA AAGAAGATAT TCTGAAACAC
    CGAGTGGTGG TGGTTACATT GAACACTTCC CAGTACCTCT GTCAGCTGGA CCTCGAACCT
    GGGTTTTTTA CTCACATCTT GTTAGACGAG GCTGCCCAGG CCATGGAGTG TGAAACCATC
    ATGCCTCTGG CACTAGCAAC TAGACACACG CGGGTTGTCC TGGCTGGCGA CCACATGCAG
    CTCAGTCCTT TTGTTTACAG CGAGTTTGCC AGGGAGAGGA ACCTTCACGT GTCGTTACTT
    GATCGGCTCT ATGAGCACTA CCCTGCCGAG TTTCCCTGTA GGATCCTCCT GTGCGAGAAC
    TACCGTTCCC ACGAAGCTAT CATCAATTAC ACCTCTGAGC TCTTCTATGA GGGCAAGCTG
    ATGGCCAGTG GAAAGCAACC AGCACACAAG GAGTTCTATC CACTAACCTT CTTTACAGCA
    CGAGGGGAAG ATGTACAGGA GAAAAATAGC ACAGCTTTTT ATAATAATGC AGAGGTATTT
    GAAGTGGTGG AGCGAGTGGA AGAGCTGAGA AGGAAGTGGC CAGTAGCGTG GGGCAAGTTG
    GATGATGGAA GTATTGGTGT GGTGACTCCA TATGCAGACC AGGTGTTCAG GATACGCGCC
    GAACTTCGAA AAAAGAGGTT ATCTGACGTT AACGTAGAAA GGGTGCTCAA TGTTCAAGGA
    AAGCAATTCA GAGTTTTGTT TCTTAGCACA GTACGTACAA GGCATACTTG TAAACATAAA
    CAGACACCAA TTAAAAAGAA GGAGCAGCTG CTAGAAGACT CCACAGAGGA CTTGGATTAT
    GGCTTTTTGT CTAACTACAA GCTCCTCAAC ACCGCCATCA CAAGAGCACA GTCCCTGGTT
    GCCGTGGTGG GGGACCCCAT CGCTCTGTGC TCCATTGGGA GATGCAGGAA ATTTTGGGAG
    CGGTTTATTG CCCTGTGTCA TGAAAACAAT AGCCTGCATG GAATCACTTT TGAACAGATC
    AAAGCGCAGC TAGAGGCTTT AGAACTAAAG AAGACATACG TGTTGAATCC ACTGGCACCT
    GAATTTATAC CCCGGGCTCT AAGAGCACAG CACGCGGGAA ACACCAACAA ACCGCAGCAA
    TCACCCCCCA AGGGAAAAAG CCTCCATCAT ACCCAGAATG ATCATTTCCA GAATGATGGA
    ATTGTTCAGC CCAATCCCTC TGTGCTTATT GGCAATCCTA TCAGAGCATA TACTCCTCCA
    CCTCCTCTTG GACCTCACCC AAATTTGGGG AAATCTCCAA GTCCTGTTCA AAGAATCGAT
    CCTCACACTG GGACGAGTAT TCTTTATGTA CCTGCTGTCT ATGGAGGGAA TGTCGTTATG
    TCGGTGCCTG TACCTGTGCC ATGGACAGGA TACCAGGGCA GGTTTGCGGT TGATCCTCGA
    ATCATTACAC ACCAGGCAGC AATGGCCTAC AACATGAACC TCTTACAGAC ACATGGACGT
    GGGTCTCCTA TTCCTTATGG CCTTGGGCAC CACCCACCTG TCAGCATAGG GCAGCCACAG
    AATCAGCATC AGGAGAAGGA TCAACATGAG CAAAGTCGCA ACGGTAAAAG TGATACAAAT
    AATCCTGGAC CTGAAATTAA TAAGATTCGA ACACCAGAGA AAAAACCTAC AGAAGCAAAA
    CAGATTGATT TGGAATCAAA CCCACAGAAC AGAAGTCCTG AATCACGTTC TGGCATTGTT
    TATCCCCATC CCAAATTTCA TCGCAAAGAT AATCTCACCC CAAGACACAT AAACCTTCCT
    CTCCCTGCCC CCCATGCCCA GTATGCCATC CCCGGTCGTC ATTTTCATCC CCTCCCCCAG
    CTCCCGAGAC CACCCTTCCC AATTCCGCAG CAGCACGCCT TGCTGAATCA GCAGCAGAGT
    AACTTGCCTG AACAGCCGAG CCCCGTGCCA GCCCAGCCCA GTCAGGTGGC CCCTCAGCTG
    GGCCCGCCGC CGCCTCAGCT CTCCCCTGCC TACCCGGCTG GCCCCGGCAG CGCGTTTTTC
    AATAACGCGG TTCCCCACCG ACCACAGTCC CCGCCTGCAG AGGCTGTGGT TCCCGAGCAG
    CAGCCCCCAC CCGTGCTGCA GGAGGGCCAC AGTCCCCTGC GGGCCCTCGC ACAGCCCGGC
    CCCATCCTGC CTTCACCTCT GAACAGCTTC CCGGACGAGA GCCCCCCGGG CCTGCCGATG
    GGGGAGGCTT TGGATCGTAT GCACGGGAGT GTGGCTCTGG AAACCCTGAG GCAGCAGCAT
    GTGCGGCTAC AGCAGTGGAG CGAGCACCAC GCCTATCTCA GTCAGGGCAG CCTCCCGTAC
    CCGCACCATC ACCACCCTCA CCCACACCTC CAGCACCTTC CCCAGCCGCC CGTTGGATTG
    CATCAGCCCC CAGTGAGAGC AGACTGGAAA CTCCCCAGCA GTGCCGAAGA TGAAGCAGAA
    ACAACTGACT CACGGTTTCA AGACTTACTC AGAGAACTGT CTAATCGTGA GCAAAGTGAA
    ACACGGGAAC TAGCTGAAAT GCCACCACCT CAATCAAGGC TTTTGCAATA TAGACAAATT
    CAGACTAGGA GCCCACCAGC AGCCCCCTCG CCCCCGTCCG GCGCAGACCA CAGCACCCAC
    TTTCCAAACT TCACTGACAG CAGCAGAGAC CTTGAAGTGG CCAACAGCCC GGCGTTCCCA
    CAGCGCCTGC CGCCCCCGAT GTTTAGCTCT CCTTTCTCGT TGCCATCTGA GCACCTCACC
    CCTCCTCTGA AATACCTGGC GCCTGATGGA GCTTGGACTT TTGCTAACTT ACAGCAGAAT
    CACCTGATGG GGCCGAGTTT CCCCTACGGC CTACCTCCAT TGCCTCACAG GCCCCCGCAG
    AACCCTTTCG TGCAAATGCA GACCCACCAG CCCGCTGTTG GCCAGGAGCC GCTCCAGCCG
    CTGTCCTCTC GGACAGTGTC GTCCTCTTCG CTCCCCAGCT TAGAAGAGTA TGAACCCAGA
    GGACCGGGTC GGCCCTTGTA CCAAAGAAGA ATCTCATCTA GCTCAGTGCA GCCTTGTTCT
    GAAGAAGCAA GCACTCCTCA AGACAGTCTT GCTCAGCGTA AAGAGCTTCA GGACCATAAT
    AACCAGTCTT CTTTCACCTT TTCCTCCCCG GAGTCCTGGG TAAACGCCAC CTCATCCACC
    CCCTATCAGA ACATTCCGTG CAATGGATCC AGCAGGCCAG CCCAGCCCAG AGAGTTGGCA
    GCTCCACCCA AGACCGTCAA AGCCCCCGAG GAACATCTGA AGCCTGAGAG CCTTGAGGGG
    TCCAGTTCCT TCAATTACAG CGTGCTGCAG CATCTCGGCC AGTTCCCCCC GCTCATGCCC
    AACAAGCAGA TCGCCGAGTC GGCCAGCAGC AGCAGCCCGC AGAGCTCCGG GGGCGGCAAG
    CCTGCCATGT CCTACGCCAG CGCCCTGCGG GCCCCTCCAA AGCCCAGGCC CCCGCCCGAG
    CAGGCCAAGA AGAGCAGCGA CCCTCTGTCT CTGTTTCAAG AACTCAGCCT GGGGAGCTCA
    GCCGGCAGCA ATGGCTTTTA CTCCTATTTT AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323779.1
    CDS219..5234
    Translation

    Target ORF information:

    RefSeq Version XM_015468293.1
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase with zinc finger (HELZ), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468293.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAAGACA GAAGAGCTGA GAAGTCATGT GAACAAGCAT GTGAATCACT GAAGAGGCAG 
    GACTATGAGA TGGCCCTCCA GCACTGCACA GAGGCCCTCC TTTCTCTTGG CCAGTACTCC
    ATGGCCGACT TTACGGGGCC CTGTCCGCTG GAGATAGAGA GCATCAAGAT CGAGAGTCTT
    CTTTATAGAA TTGCCTCCTT TTTGCAGCTG AAAAACTATG GGCAAGCTGA TGAAGATTGT
    AGGCATGTGC TGGGAGAAGG ACTGGCCAAG GGAGATGGCG CCTTTCAGGC AGTGCTTTGC
    TGCATGCAGC TTAAAGGAAA GCTCCAGCCT GTATCCCACA TTCTTGCCAA GTCACTGACA
    GGAGAGTCCC TGAATGGGAT GGTAACAAAA GATTTGACAA GGCTAAAAAC ACTTCTCAAA
    GAAACAGAGA CAGCAACTAG TAACGTCCTC TCTGGATATC ATGTGGAAGA CTTAGAGGAG
    GGATCTTGTA ATGGTTGGCA TTTCCGCCCA CCACCTAGGG GAATCACAAG CAGCGAGGAA
    TATACTTTGT GTAAAAGATT TTTAGAGCAA GGAATCTGTA GGTATGGTGC GCAGTGTACT
    TCAGCACATT CCCAGGAAGA ACTAGCAGAA TGGCAGAAAA GATATGCTTC ACGGTTGATA
    AAATTGAAAC AGCAAAATGA GAATAAACAG CTCTCAGGCA GTTACATGGA AACCTTGATA
    GAAAAGTGGA TGAATTCATT ATCTCCTGAG AAAGTGCTCA GTGAGTGTGT GGAAGGAGTG
    AAGGTGGAGC ATGACCCCGG TCTGTCTGTC ACCGTCAACA CCAAAAAGTC CCACCAGACC
    TGGACCTTCG CTCTCACCTG TAAGCCTGCA AGAATGCTGT GTCGTGTAGC ATTGCTTTAT
    GATGCTCATC GTCCTCATTT TAGTATCATT GCAATATCTG CCGGAGATAG TACTACCCAG
    GTATCACAAG AAGTCCCAGA AAACTGTCAA GAATGGATAG GAGGGAAGAT GGCCCCAAAT
    GGATTAGATC ATTACGTGTA TAAAGTCGGG ATAGCATTTA ACACAGAAAT ATTTGGAACT
    TTTCGCCAAA CCATAGTTTT CGACTTTGGA TTGGAACCAG TACTCATGCA AAGAGTAATG
    ATTGATGCAG CTTCTACAGA AGATCTTGAG TACCTGATGC ATGCAAATCT CCCCCCTGCG
    CCTCTCCATT GCCCGGTCTC CCCCCTGCGC CTCTCCATTG CCCGGTCTCC CCCCTGCGCC
    TCTCCATTGC CCGGTCTCCC CGCTGCGCCT CTCCGTTGCC TGGTCTCCCC CCTGCGCCTC
    TCCGTTGCCC AGTCTCCCCT CTGCGCCTCT CGATTGCCCG GACATGTTAT CTTCTTTTAT
    AGGGTATATT TCAGAAACCG CTGGGTGAAG ACTGTCCACC CAGTTGTGCA TCAGTACTGT
    TTGATCTCAA GCGCACATTC CACCTTTCAG ATGCCACAGA AAGAAGATAT TCTGAAACAC
    CGAGTGGTGG TGGTTACATT GAACACTTCC CAGTACCTCT GTCAGCTGGA CCTCGAACCT
    GGGTTTTTTA CTCACATCTT GTTAGACGAG GCTGCCCAGG CCATGGAGTG TGAAACCATC
    ATGCCTCTGG CACTAGCAAC TAGACACACG CGGGTTGTCC TGGCTGGCGA CCACATGCAG
    CTCAGTCCTT TTGTTTACAG CGAGTTTGCC AGGGAGAGGA ACCTTCACGT GTCGTTACTT
    GATCGGCTCT ATGAGCACTA CCCTGCCGAG TTTCCCTGTA GGATCCTCCT GTGCGAGAAC
    TACCGTTCCC ACGAAGCTAT CATCAATTAC ACCTCTGAGC TCTTCTATGA GGGCAAGCTG
    ATGGCCAGTG GAAAGCAACC AGCACACAAG GAGTTCTATC CACTAACCTT CTTTACAGCA
    CGAGGGGAAG ATGTACAGGA GAAAAATAGC ACAGCTTTTT ATAATAATGC AGAGGTATTT
    GAAGTGGTGG AGCGAGTGGA AGAGCTGAGA AGGAAGTGGC CAGTAGCGTG GGGCAAGTTG
    GATGATGGAA GTATTGGTGT GGTGACTCCA TATGCAGACC AGGTGTTCAG GATACGCGCC
    GAACTTCGAA AAAAGAGGTT ATCTGACGTT AACGTAGAAA GGGTGCTCAA TGTTCAAGGA
    AAGCAATTCA GAGTTTTGTT TCTTAGCACA GTACGTACAA GGCATACTTG TAAACATAAA
    CAGACACCAA TTAAAAAGAA GGAGCAGCTG CTAGAAGACT CCACAGAGGA CTTGGATTAT
    GGCTTTTTGT CTAACTACAA GCTCCTCAAC ACCGCCATCA CAAGAGCACA GTCCCTGGTT
    GCCGTGGTGG GGGACCCCAT CGCTCTGTGC TCCATTGGGA GATGCAGGAA ATTTTGGGAG
    CGGTTTATTG CCCTGTGTCA TGAAAACAAT AGCCTGCATG GAATCACTTT TGAACAGATC
    AAAGCGCAGC TAGAGGCTTT AGAACTAAAG AAGACATACG TGTTGAATCC ACTGGCACCT
    GAATTTATAC CCCGGGCTCT AAGAGCACAG CACGCGGGAA ACACCAACAA ACCGCAGCAA
    TCACCCCCCA AGGGAAAAAG CCTCCATCAT ACCCAGAATG ATCATTTCCA GAATGATGGA
    ATTGTTCAGC CCAATCCCTC TGTGCTTATT GGCAATCCTA TCAGAGCATA TACTCCTCCA
    CCTCCTCTTG GACCTCACCC AAATTTGGGG AAATCTCCAA GTCCTGTTCA AAGAATCGAT
    CCTCACACTG GGACGAGTAT TCTTTATGTA CCTGCTGTCT ATGGAGGGAA TGTCGTTATG
    TCGGTGCCTG TACCTGTGCC ATGGACAGGA TACCAGGGCA GGTTTGCGGT TGATCCTCGA
    ATCATTACAC ACCAGGCAGC AATGGCCTAC AACATGAACC TCTTACAGAC ACATGGACGT
    GGGTCTCCTA TTCCTTATGG CCTTGGGCAC CACCCACCTG TCAGCATAGG GCAGCCACAG
    AATCAGCATC AGGAGAAGGA TCAACATGAG CAAAGTCGCA ACGGTAAAAG TGATACAAAT
    AATCCTGGAC CTGAAATTAA TAAGATTCGA ACACCAGAGA AAAAACCTAC AGAAGCAAAA
    CAGATTGATT TGGAATCAAA CCCACAGAAC AGAAGTCCTG AATCACGTTC TGGCATTGTT
    TATCCCCATC CCAAATTTCA TCGCAAAGAT AATCTCACCC CAAGACACAT AAACCTTCCT
    CTCCCTGCCC CCCATGCCCA GTATGCCATC CCCGGTCGTC ATTTTCATCC CCTCCCCCAG
    CTCCCGAGAC CACCCTTCCC AATTCCGCAG CAGCACGCCT TGCTGAATCA GCAGCAGAGT
    AACTTGCCTG AACAGCCGAG CCCCGTGCCA GCCCAGCCCA GTCAGGTGGC CCCTCAGCTG
    GGCCCGCCGC CGCCTCAGCT CTCCCCTGCC TACCCGGCTG GCCCCGGCAG CGCGTTTTTC
    AATAACGCGG TTCCCCACCG ACCACAGTCC CCGCCTGCAG AGGCTGTGGT TCCCGAGCAG
    CAGCCCCCAC CCGTGCTGCA GGAGGGCCAC AGTCCCCTGC GGGCCCTCGC ACAGCCCGGC
    CCCATCCTGC CTTCACCTCT GAACAGCTTC CCGGACGAGA GCCCCCCGGG CCTGCCGATG
    GGGGAGGCTT TGGATCGTAT GCACGGGAGT GTGGCTCTGG AAACCCTGAG GCAGCAGCAT
    GTGCGGCTAC AGCAGTGGAG CGAGCACCAC GCCTATCTCA GTCAGGGCAG CCTCCCGTAC
    CCGCACCATC ACCACCCTCA CCCACACCTC CAGCACCTTC CCCAGCCGCC CGTTGGATTG
    CATCAGCCCC CAGTGAGAGC AGACTGGAAA CTCCCCAGCA GTGCCGAAGA TGAAGCAGAA
    ACAACTGACT CACGGTTTCA AGACTTACTC AGAGAACTGT CTAATCGTGA GCAAAGTGAA
    ACACGGGAAC TAGCTGAAAT GCCACCACCT CAATCAAGGC TTTTGCAATA TAGACAAATT
    CAGACTAGGA GCCCACCAGC AGCCCCCTCG CCCCCGTCCG GCGCAGACCA CAGCACCCAC
    TTTCCAAACT TCACTGACAG CAGCAGAGAC CTTGAAGTGG CCAACAGCCC GGCGTTCCCA
    CAGCGCCTGC CGCCCCCGAT GTTTAGCTCT CCTTTCTCGT TGCCATCTGA GCACCTCACC
    CCTCCTCTGA AATACCTGGC GCCTGATGGA GCTTGGACTT TTGCTAACTT ACAGCAGAAT
    CACCTGATGG GGCCGAGTTT CCCCTACGGC CTACCTCCAT TGCCTCACAG GCCCCCGCAG
    AACCCTTTCG TGCAAATGCA GACCCACCAG CCCGCTGTTG GCCAGGAGCC GCTCCAGCCG
    CTGTCCTCTC GGACAGTGTC GTCCTCTTCG CTCCCCAGCT TAGAAGAGTA TGAACCCAGA
    GGACCGGGTC GGCCCTTGTA CCAAAGAAGA ATCTCATCTA GCTCAGTGCA GCCTTGTTCT
    GAAGAAGCAA GCACTCCTCA AGACAGTCTT GCTCAGCGTA AAGAGCTTCA GGACCATAAT
    AACCAGTCTT CTTTCACCTT TTCCTCCCCG GAGTCCTGGG TAAACGCCAC CTCATCCACC
    CCCTATCAGA ACATTCCGTG CAATGGATCC AGCAGGCCAG CCCAGCCCAG AGAGTTGGCA
    GCTCCACCCA AGACCGTCAA AGCCCCCGAG GAACATCTGA AGCCTGAGAG CCTTGAGGGG
    TCCAGTTCCT TCAATTACAG CGTGCTGCAG CATCTCGGCC AGTTCCCCCC GCTCATGCCC
    AACAAGCAGA TCGCCGAGTC GGCCAGCAGC AGCAGCCCGC AGAGCTCCGG GGGCGGCAAG
    CCTGCCATGT CCTACGCCAG CGCCCTGCGG GCCCCTCCAA AGCCCAGGCC CCCGCCCGAG
    CAGGCCAAGA AGAGCAGCGA CCCTCTGTCT CTGTTTCAAG AACTCAGCCT GGGGAGCTCA
    GCCGGCAGCA ATGGCTTTTA CTCCTATTTT AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.