SREBF1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SREBF1
Entrez Gene ID	539361
Full Name	sterol regulatory element binding transcription factor 1
Synonyms	ADD1,SREBP-1,SREBP1
General protein information	Preferred Names sterol regulatory element binding transcription factor 1 Names sterol regulatory element-binding protein 1 adipocyte determination and differentiation-dependent factor 1 sterol response element binding protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024980342.1	XP_024836110.1	sterol regulatory element-binding protein 1 isoform X1
XM_024980343.1	XP_024836111.1	sterol regulatory element-binding protein 1 isoform X2
NM_001113302.1	NP_001106773.1	sterol regulatory element-binding protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SREBF1	XP_003315641.1
Mus musculus (house mouse)	Srebf1	NP_035610.1
Rattus norvegicus (Norway rat)	Srebf1	NP_001263636.1
Danio rerio (zebrafish)	srebf1	NP_001098599.1
Drosophila melanogaster (fruit fly)	HLH106	NP_001262064.1
Homo sapiens (human)	SREBF1	NP_001005291.1
Canis lupus familiaris (dog)	SREBF1	NP_001184012.1
Gallus gallus (chicken)	SREBF1	NP_989457.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000076	XP_311076.5
	SREBF1	XP_001095392.1
Xenopus tropicalis (tropical clawed frog)	srebf1	XP_002935886.1
Bos taurus (cattle)	SREBF1	NP_001106773.1

Gene Ontology
Bibliography

Related articles in PubMed

Epigenetic mechanisms contribute to decrease stearoyl-CoA desaturase 1 expression in the liver of dairy cows after prolonged feeding of high-concentrate diet.
Xu TL, Seyfert HM, Shen XZ
Journal of dairy science(2017 Dec)

Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression.
Prodanovi? R, Kori?anac G, Vujanac I, Djordjevi? A, Panteli? M, Romi? S, Stanimirovi? Z, Kirovski D
Research in veterinary science10716-9(2016 Aug)

Stearoyl-CoA desaturase 1 expression is downregulated in liver and udder during E. coli mastitis through enhanced expression of repressive C/EBP factors and reduced expression of the inducer SREBP1A.
Xu T, Shen X, Seyfert HM
BMC molecular biology17(1)16(2016 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.
Title: Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression.

SREBP1a activated while C/EBP factors downregulated the activity of the SCD1 promoter.
Title: Stearoyl-CoA desaturase 1 expression is downregulated in liver and udder during E. coli mastitis through enhanced expression of repressive C/EBP factors and reduced expression of the inducer SREBP1A.

Hepatic SREBP-1c-mediated lipid synthesis and the NF-kappaB inflammatory pathway were both overinduced in cows with fatty liver.
Title: SREBP-1c overactivates ROS-mediated hepatic NF-?B inflammatory pathway in dairy cows with fatty liver.

SREBP1 was found to be a key positive regulator of milk fat synthesis and was shown to be regulated by stearic acid and serum.
Title: Function of SREBP1 in the milk fat synthesis of dairy cow mammary epithelial cells.

data suggest that low SREBP-1c expression can decrease lipid synthesis, increase lipid oxidation, and decrease the TG and VLDL content in bovine hepatocytes
Title: SREBP-1c gene silencing can decrease lipid deposits in bovine hepatocytes cultured in vitro.

The following SREBF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SREBF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa226485	XM_024980342.1 Latest version!	Bos taurus sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa226486	XM_024980343.1 Latest version!	Bos taurus sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa40072	NM_001113302.1 Latest version!	Bos taurus sterol regulatory element binding transcription factor 1 (SREBF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SREBF1 ( XM_024980342.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836110.1 Bos taurus sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226485
Clone ID Related Accession (Same CDS sequence)	XM_024980342.1
Accession Version	XM_024980342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sterol regulatory element-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGCA CGTTCGAAGA CATGCTTCAG CTCATCAACA ACCAAGACAG CGAATTCCCG 
GGCCTATTCG ACCCGCCCTA CGCTGGGGGC GGAGCAGGGA CCACAGACCC TGCCAGTCCT 
GACGCCAGCT CCCCGGGCAG CCTGTCCCCA CCTCCTTCCA CGATGAGCTC CTCACTTGAA 
GGCTTCCTGG GGGTGACCAA GGCGACACCC CCACCCTTGT CCCCTCCCCA GCCTGCGCCC 
ACCCCCCTGA AGATATACCC ATCTGTGCCT GCCTTCTCCC CGGTGCCTGG GATCAAGGAG 
GAGCCAGCGC CCCTGACTGT CCTCCAGCCC ACCACCCCCC AGCCCCTGCC TGGAGCTCTC 
CTGCCGCAGA GTGTTGCGGC CACCACCCCA CCGCAGTTCA GCTCTGCTCC CATTGTGGGC 
TACCCCAGCC CTCCGGGAGG CTTCACAGGG ACCCCTCCGG GGAGCAGCTC GCAGTCACTG 
GCTGGCCTGC CACTGGCTTC CCTGCCAGGG GTCCCGCCCG TCTCCTTGCA CAGCCAGGTT 
CAGAGCGCGG CCCCCCAGCA GCTGTTGACA GCCACAGCCA CCCCCACGGT GGCCCCTGGA 
GCAACCGCTG TGACCTCCCA GATACAGCAG GTCCCGGTCC TGCTGCAGCC CCACTTCATC 
AAGGCAGACT CGTTGCTCCT GACGACCGTG AAAACAGATG TGGGAGCCCC CTTGAAAGCG 
GCAGGCATCC GCTCCCTGGG CCCTGGCACT GCTGTTCAGG CAGCGCCCTT GCAGACCCTG 
GTGAGTGGCG GGGCCATCCT GGCCACGGTG CCACTGGTAG TAGACACTGA CAAGCTGCCC 
ATCAACCGAC TTGCCAGTGG CAAGGCTCCA GGCTCGGCGC AGAGCCGCGG CGAGAAGCGT 
ACAGCCCACA ACGCCATCGA GAAACGCTAC CGCTCTTCCA TCAATGACAA GATCGTTGAG 
CTCAAGGACC TGGTGGTGGG CACCGAGGCC AAGTTGAATA AATCTGCCGT CTTGCGCAAG 
GCCATCGACT ACATCCGCTT CCTTCAGCAC AGCAACCAGA AGCTCAAGCA GGAGAACCTG 
AGTCTGCGCA CTGCTGTCCA CAAAAGCAAA TCGCTGAAGG ACCTGGTGTC GGCTTGTGGC 
AGTGGAGGGA GCACAGATGT GCCCATGGAG GGCATGAAGC CAGAGGTGGT CGACACCCTG 
AGTCCACCCC CCTCAGATGC CGGCTCGCCC TCCCAGAGCA GCCCCTTGTC CCTTGGCAGC 
AGGGGCAGTA GCAGCGGTGG AAGTGGCAGT GACTCGGAGC CCGACAGCCC GGTCTTTGAG 
GACGGCCAGG TGAATCCAGA GCCGCTGCCT GCTCCCCACA GCCAGGGCAT GCTGGACCGC 
TCTCGCCTGG CCCTGTGCGC GCTCGTCTTC CTCTGTCTCT CCTGCAACCC CTTGGCCTCC 
CTGCTGGGTA GCCGGGGTCC TGCTGGCCCC TCCGACACCA CCAGCATCAA CCACGGTCCC 
AGGCGCAGCA TGCTGGGTGC TGAGGGCAGA GATGGTCCTG GCTGGGCCCC ATGGCTGCTG 
CCCCCACTGG TCTGGCTGAT GAATGGGCTG CTGGTGCTCT TCTCCTTGGC ACTTCTCTTT 
GTCTATGGAG AACCAGTCAC TCGGCCCCAC TCATGCCCCG CCGTGCACTT CTGGAGGCAT 
CGCAAGCAGG CTGACCTGGA CCTGGCCAGG GGGGACTTTG CCCAGGCTGC CCAGCAGCTG 
TGGCTGGCCC TGCGGGCCTT GGGCCGGCCT CTGCCCACCT CCCACCTGGA CCTGGCCTGC 
AGCCTGCTCT GGAGCCTCAT CCGCCACCTG CTGCAACGTC TCTGGGTGGG CCGCTGGCTG 
GCCGGCTGGG CAGGGGGCCT ACGGAGGGAC AGGGCCCTAC AGGCAGATGC TCGCACCAGT 
GCCCGTGACG CAGCCCTCGT CTACCACAAG CTGCACCAGC TGCACACCAT GGGAAAGTAC 
TCAGGTGGGC ACCTCGCTGC TGCCAACCTG GCACTGAGTG CCCTGAACCT GGCCGAGTGC 
GCTGGAGATG CTGTGTCCGT GGCCACGCTG GCTGAGATCT ACGTGGCCGC CGCACTCAGG 
GTCAAGGCCA GTCTGCCCCG GGCCTTGCAT TTTCTGACAC GCTTCTTCCT GAGCAGTGCT 
CGCCAGGCCT GCCTGGCACA GAGCGGCTCA GTGCCCCTTG CCATGCAGTG GCTCTGCCAC 
CCTGTGGGCC ACCGTTTCTT CGTGGATGGC AACTGGGCCC TGTGCAGCGC CCCGAGGGAC 
AGCTTGTACA GCGTGGCTGG GAACCCAGTG GATCCCCTGG CCCAGGTGAC TCAACTGTTC 
CGCGAACATC TCTTGGAGCG AGCACTGAAT TGCGTGGCCC AGCCCAGCCC CAGTCCTGGA 
TCAGCCGAGG GGGACAAGGA GTTCTCAGAT GCCCTGGGGT ACCTGCAGCT GCTGAACAGC 
TGTTCGGACG TGGCCGGAGC TCCTACCTGC AGCTTCTCCA TCAGCTCCAG CATGGCTGCC 
ACCCCCGGCA CAGACCCGGT GGCCAAGTGG TGGGCCTCTC TGACAGCTGT GGTGACCCAC 
TGGCTTCGGC GGGATGAGGA GGCAGCCGAG AGGCTGTACC CATTGGTGGA GCACCTGCCC 
CGTGCCCTGC AGGAGTCCGA GAAACCCCTA CCCAGGGCGG CTCTGCACTC CTTCAAGGCT 
GCCCGGACCA TTCTAGGCCG CGGGAAGGCT GAGTCTGGCC CAGCCAGCCT GGCAATGTGT 
GAGAAGGCCA GTGGGTACCT GCAGGACAGC CTGGCCACCA CACCAGCTGA CAGCTCCATT 
GACAAGGCCT TGCAGCTGCT CCTGTGTGAC CTGCTCCTTG TGGCGCGCAC CAGCCTCTGG 
CAGCGGCAGA AGCTGCCGGC ACCCACCCAG GCCTCGCAGG GCCCTGGAGG TGGGGCCCAG 
GCCTCTGCCC TCGAGCTTCG TGGTTTCCAG AGGGACCTGA GTGGCCTGAG GCGTCTGGCA 
CAGAATGTTC GGCCTGCCAT GCGGAGGGTA TTCCTACATG AAGCCACTGC CCGACTGATG 
GCAGGGGCCA GCCCAGCGCG GACACACCAG CTTCTGGATC GCAGCCTGAG GAGGAGGGTC 
GGCCCCTGCA AAGGAGGTGC GGTGGCGGCG GAACTGGAGT CCCGGCCCAC GAGGCGGGAG 
CAGGCCGAGG CTTTGCTGCT GGCCTCTTGC TACCTGCCGC CCGGCTTCCT ATCGGCGCCC 
GGGCAGCGCG TGGGCATGCT GGCCGAGGCG GCGCGCACGC TGGAGAAGCT CGGTGACCGC 
CGGCTGCTGC ACGACTGTCA GCAGATGCTC ATGCGCCTGG GCGGTGGGAC CACTGTGACC 
TCCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836110.1
CDS	358..3726
Translation	MDCTFEDMLQLINNQDSEFPGLFDPPYAGGGAGTTDPASPDASSPGSLSPPPSTMSSSLEGFLGVTKATPPPLSPPQPAPTPLKIYPSVPAFSPVPGIKEEPAPLTVLQPTTPQPLPGALLPQSVAATTPPQFSSAPIVGYPSPPGGFTGTPPGSSSQSLAGLPLASLPGVPPVSLHSQVQSAAPQQLLTATATPTVAPGATAVTSQIQQVPVLLQPHFIKADSLLLTTVKTDVGAPLKAAGIRSLGPGTAVQAAPLQTLVSGGAILATVPLVVDTDKLPINRLASGKAPGSAQSRGEKRTAHNAIEKRYRSSINDKIVELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLSLRTAVHKSKSLKDLVSACGSGGSTDVPMEGMKPEVVDTLSPPPSDAGSPSQSSPLSLGSRGSSSGGSGSDSEPDSPVFEDGQVNPEPLPAPHSQGMLDRSRLALCALVFLCLSCNPLASLLGSRGPAGPSDTTSINHGPRRSMLGAEGRDGPGWAPWLLPPLVWLMNGLLVLFSLALLFVYGEPVTRPHSCPAVHFWRHRKQADLDLARGDFAQAAQQLWLALRALGRPLPTSHLDLACSLLWSLIRHLLQRLWVGRWLAGWAGGLRRDRALQADARTSARDAALVYHKLHQLHTMGKYSGGHLAAANLALSALNLAECAGDAVSVATLAEIYVAAALRVKASLPRALHFLTRFFLSSARQACLAQSGSVPLAMQWLCHPVGHRFFVDGNWALCSAPRDSLYSVAGNPVDPLAQVTQLFREHLLERALNCVAQPSPSPGSAEGDKEFSDALGYLQLLNSCSDVAGAPTCSFSISSSMAATPGTDPVAKWWASLTAVVTHWLRRDEEAAERLYPLVEHLPRALQESEKPLPRAALHSFKAARTILGRGKAESGPASLAMCEKASGYLQDSLATTPADSSIDKALQLLLCDLLLVARTSLWQRQKLPAPTQASQGPGGGAQASALELRGFQRDLSGLRRLAQNVRPAMRRVFLHEATARLMAGASPARTHQLLDRSLRRRVGPCKGGAVAAELESRPTRREQAEALLLASCYLPPGFLSAPGQRVGMLAEAARTLEKLGDRRLLHDCQQMLMRLGGGTTVTSS

Target ORF information:

RefSeq Version	XM_024980342.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980342.1

ORF Insert Sequence:

ATGGATTGCA CGTTCGAAGA CATGCTTCAG CTCATCAACA ACCAAGACAG CGAATTCCCG 
GGCCTATTCG ACCCGCCCTA CGCTGGGGGC GGAGCAGGGA CCACAGACCC TGCCAGTCCT 
GACGCCAGCT CCCCGGGCAG CCTGTCCCCA CCTCCTTCCA CGATGAGCTC CTCACTTGAA 
GGCTTCCTGG GGGTGACCAA GGCGACACCC CCACCCTTGT CCCCTCCCCA GCCTGCGCCC 
ACCCCCCTGA AGATATACCC ATCTGTGCCT GCCTTCTCCC CGGTGCCTGG GATCAAGGAG 
GAGCCAGCGC CCCTGACTGT CCTCCAGCCC ACCACCCCCC AGCCCCTGCC TGGAGCTCTC 
CTGCCGCAGA GTGTTGCGGC CACCACCCCA CCGCAGTTCA GCTCTGCTCC CATTGTGGGC 
TACCCCAGCC CTCCGGGAGG CTTCACAGGG ACCCCTCCGG GGAGCAGCTC GCAGTCACTG 
GCTGGCCTGC CACTGGCTTC CCTGCCAGGG GTCCCGCCCG TCTCCTTGCA CAGCCAGGTT 
CAGAGCGCGG CCCCCCAGCA GCTGTTGACA GCCACAGCCA CCCCCACGGT GGCCCCTGGA 
GCAACCGCTG TGACCTCCCA GATACAGCAG GTCCCGGTCC TGCTGCAGCC CCACTTCATC 
AAGGCAGACT CGTTGCTCCT GACGACCGTG AAAACAGATG TGGGAGCCCC CTTGAAAGCG 
GCAGGCATCC GCTCCCTGGG CCCTGGCACT GCTGTTCAGG CAGCGCCCTT GCAGACCCTG 
GTGAGTGGCG GGGCCATCCT GGCCACGGTG CCACTGGTAG TAGACACTGA CAAGCTGCCC 
ATCAACCGAC TTGCCAGTGG CAAGGCTCCA GGCTCGGCGC AGAGCCGCGG CGAGAAGCGT 
ACAGCCCACA ACGCCATCGA GAAACGCTAC CGCTCTTCCA TCAATGACAA GATCGTTGAG 
CTCAAGGACC TGGTGGTGGG CACCGAGGCC AAGTTGAATA AATCTGCCGT CTTGCGCAAG 
GCCATCGACT ACATCCGCTT CCTTCAGCAC AGCAACCAGA AGCTCAAGCA GGAGAACCTG 
AGTCTGCGCA CTGCTGTCCA CAAAAGCAAA TCGCTGAAGG ACCTGGTGTC GGCTTGTGGC 
AGTGGAGGGA GCACAGATGT GCCCATGGAG GGCATGAAGC CAGAGGTGGT CGACACCCTG 
AGTCCACCCC CCTCAGATGC CGGCTCGCCC TCCCAGAGCA GCCCCTTGTC CCTTGGCAGC 
AGGGGCAGTA GCAGCGGTGG AAGTGGCAGT GACTCGGAGC CCGACAGCCC GGTCTTTGAG 
GACGGCCAGG TGAATCCAGA GCCGCTGCCT GCTCCCCACA GCCAGGGCAT GCTGGACCGC 
TCTCGCCTGG CCCTGTGCGC GCTCGTCTTC CTCTGTCTCT CCTGCAACCC CTTGGCCTCC 
CTGCTGGGTA GCCGGGGTCC TGCTGGCCCC TCCGACACCA CCAGCATCAA CCACGGTCCC 
AGGCGCAGCA TGCTGGGTGC TGAGGGCAGA GATGGTCCTG GCTGGGCCCC ATGGCTGCTG 
CCCCCACTGG TCTGGCTGAT GAATGGGCTG CTGGTGCTCT TCTCCTTGGC ACTTCTCTTT 
GTCTATGGAG AACCAGTCAC TCGGCCCCAC TCATGCCCCG CCGTGCACTT CTGGAGGCAT 
CGCAAGCAGG CTGACCTGGA CCTGGCCAGG GGGGACTTTG CCCAGGCTGC CCAGCAGCTG 
TGGCTGGCCC TGCGGGCCTT GGGCCGGCCT CTGCCCACCT CCCACCTGGA CCTGGCCTGC 
AGCCTGCTCT GGAGCCTCAT CCGCCACCTG CTGCAACGTC TCTGGGTGGG CCGCTGGCTG 
GCCGGCTGGG CAGGGGGCCT ACGGAGGGAC AGGGCCCTAC AGGCAGATGC TCGCACCAGT 
GCCCGTGACG CAGCCCTCGT CTACCACAAG CTGCACCAGC TGCACACCAT GGGAAAGTAC 
TCAGGTGGGC ACCTCGCTGC TGCCAACCTG GCACTGAGTG CCCTGAACCT GGCCGAGTGC 
GCTGGAGATG CTGTGTCCGT GGCCACGCTG GCTGAGATCT ACGTGGCCGC CGCACTCAGG 
GTCAAGGCCA GTCTGCCCCG GGCCTTGCAT TTTCTGACAC GCTTCTTCCT GAGCAGTGCT 
CGCCAGGCCT GCCTGGCACA GAGCGGCTCA GTGCCCCTTG CCATGCAGTG GCTCTGCCAC 
CCTGTGGGCC ACCGTTTCTT CGTGGATGGC AACTGGGCCC TGTGCAGCGC CCCGAGGGAC 
AGCTTGTACA GCGTGGCTGG GAACCCAGTG GATCCCCTGG CCCAGGTGAC TCAACTGTTC 
CGCGAACATC TCTTGGAGCG AGCACTGAAT TGCGTGGCCC AGCCCAGCCC CAGTCCTGGA 
TCAGCCGAGG GGGACAAGGA GTTCTCAGAT GCCCTGGGGT ACCTGCAGCT GCTGAACAGC 
TGTTCGGACG TGGCCGGAGC TCCTACCTGC AGCTTCTCCA TCAGCTCCAG CATGGCTGCC 
ACCCCCGGCA CAGACCCGGT GGCCAAGTGG TGGGCCTCTC TGACAGCTGT GGTGACCCAC 
TGGCTTCGGC GGGATGAGGA GGCAGCCGAG AGGCTGTACC CATTGGTGGA GCACCTGCCC 
CGTGCCCTGC AGGAGTCCGA GAAACCCCTA CCCAGGGCGG CTCTGCACTC CTTCAAGGCT 
GCCCGGACCA TTCTAGGCCG CGGGAAGGCT GAGTCTGGCC CAGCCAGCCT GGCAATGTGT 
GAGAAGGCCA GTGGGTACCT GCAGGACAGC CTGGCCACCA CACCAGCTGA CAGCTCCATT 
GACAAGGCCT TGCAGCTGCT CCTGTGTGAC CTGCTCCTTG TGGCGCGCAC CAGCCTCTGG 
CAGCGGCAGA AGCTGCCGGC ACCCACCCAG GCCTCGCAGG GCCCTGGAGG TGGGGCCCAG 
GCCTCTGCCC TCGAGCTTCG TGGTTTCCAG AGGGACCTGA GTGGCCTGAG GCGTCTGGCA 
CAGAATGTTC GGCCTGCCAT GCGGAGGGTA TTCCTACATG AAGCCACTGC CCGACTGATG 
GCAGGGGCCA GCCCAGCGCG GACACACCAG CTTCTGGATC GCAGCCTGAG GAGGAGGGTC 
GGCCCCTGCA AAGGAGGTGC GGTGGCGGCG GAACTGGAGT CCCGGCCCAC GAGGCGGGAG 
CAGGCCGAGG CTTTGCTGCT GGCCTCTTGC TACCTGCCGC CCGGCTTCCT ATCGGCGCCC 
GGGCAGCGCG TGGGCATGCT GGCCGAGGCG GCGCGCACGC TGGAGAAGCT CGGTGACCGC 
CGGCTGCTGC ACGACTGTCA GCAGATGCTC ATGCGCCTGG GCGGTGGGAC CACTGTGACC 
TCCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SREBF1 ( XM_024980343.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836111.1 Bos taurus sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa226486
Clone ID Related Accession (Same CDS sequence)	XM_024980343.1
Accession Version	XM_024980343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sterol regulatory element-binding protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGC CACCCTTCAA CGAAGCGGCC TTGGAGCTGG CGCTGGCCGA GCCGTGCGAG 
CTGGACGCGG CGCTGCTGAC CGACATAGAA GGTGCGTCAG GGCCACTGGA CTCCGCGCAC 
GGGCGGCGCC GGGCCGGGGG CGCGGAGGGC CCACGGGCGA CGGGCCCGGG CCGCGGAACC 
ATGGATTGCA CGTTCGAAGA CATGCTTCAG CTCATCAACA ACCAAGACAG CGAATTCCCG 
GGCCTATTCG ACCCGCCCTA CGCTGGGGGC GGAGCAGGGA CCACAGACCC TGCCAGTCCT 
GACGCCAGCT CCCCGGGCAG CCTGTCCCCA CCTCCTTCCA CGATGAGCTC CTCACTTGAA 
GGCTTCCTGG GGGTGACCAA GGCGACACCC CCACCCTTGT CCCCTCCCCA GCCTGCGCCC 
ACCCCCCTGA AGATATACCC ATCTGTGCCT GCCTTCTCCC CGGTGCCTGG GATCAAGGAG 
GAGCCAGCGC CCCTGACTGT CCTCCAGCCC ACCACCCCCC AGCCCCTGCC TGGAGCTCTC 
CTGCCGCAGA GTGTTGCGGC CACCACCCCA CCGCAGTTCA GCTCTGCTCC CATTGTGGGC 
TACCCCAGCC CTCCGGGAGG CTTCACAGGG ACCCCTCCGG GGAGCAGCTC GCAGTCACTG 
GCTGGCCTGC CACTGGCTTC CCTGCCAGGG GTCCCGCCCG TCTCCTTGCA CAGCCAGGTT 
CAGAGCGCGG CCCCCCAGCA GCTGTTGACA GCCACAGCCA CCCCCACGGT GGCCCCTGGA 
GCAACCGCTG TGACCTCCCA GATACAGCAG GTCCCGGTCC TGCTGCAGCC CCACTTCATC 
AAGGCAGACT CGTTGCTCCT GACGACCGTG AAAACAGATG TGGGAGCCCC CTTGAAAGCG 
GCAGGCATCC GCTCCCTGGG CCCTGGCACT GCTGTTCAGG CAGCGCCCTT GCAGACCCTG 
GTGAGTGGCG GGGCCATCCT GGCCACGGTG CCACTGGTAG TAGACACTGA CAAGCTGCCC 
ATCAACCGAC TTGCCAGTGG CAAGGCTCCA GGCTCGGCGC AGAGCCGCGG CGAGAAGCGT 
ACAGCCCACA ACGCCATCGA GAAACGCTAC CGCTCTTCCA TCAATGACAA GATCGTTGAG 
CTCAAGGACC TGGTGGTGGG CACCGAGGCC AAGTTGAATA AATCTGCCGT CTTGCGCAAG 
GCCATCGACT ACATCCGCTT CCTTCAGCAC AGCAACCAGA AGCTCAAGCA GGAGAACCTG 
AGTCTGCGCA CTGCTGTCCA CAAAAGCAAA TCGCTGAAGG ACCTGGTGTC GGCTTGTGGC 
AGTGGAGGGA GCACAGATGT GCCCATGGAG GGCATGAAGC CAGAGGTGGT CGACACCCTG 
AGTCCACCCC CCTCAGATGC CGGCTCGCCC TCCCAGAGCA GCCCCTTGTC CCTTGGCAGC 
AGGGGCAGTA GCAGCGGTGG AAGTGGCAGT GACTCGGAGC CCGACAGCCC GGTCTTTGAG 
GACGGCCAGG TGAATCCAGA GCCGCTGCCT GCTCCCCACA GCCAGGGCAT GCTGGACCGC 
TCTCGCCTGG CCCTGTGCGC GCTCGTCTTC CTCTGTCTCT CCTGCAACCC CTTGGCCTCC 
CTGCTGGGTA GCCGGGGTCC TGCTGGCCCC TCCGACACCA CCAGCATCAA CCACGGTCCC 
AGGCGCAGCA TGCTGGGTGC TGAGGGCAGA GATGGTCCTG GCTGGGCCCC ATGGCTGCTG 
CCCCCACTGG TCTGGCTGAT GAATGGGCTG CTGGTGCTCT TCTCCTTGGC ACTTCTCTTT 
GTCTATGGAG AACCAGTCAC TCGGCCCCAC TCATGCCCCG CCGTGCACTT CTGGAGGCAT 
CGCAAGCAGG CTGACCTGGA CCTGGCCAGG GGGGACTTTG CCCAGGCTGC CCAGCAGCTG 
TGGCTGGCCC TGCGGGCCTT GGGCCGGCCT CTGCCCACCT CCCACCTGGA CCTGGCCTGC 
AGCCTGCTCT GGAGCCTCAT CCGCCACCTG CTGCAACGTC TCTGGGTGGG CCGCTGGCTG 
GCCGGCTGGG CAGGGGGCCT ACGGAGGGAC AGGGCCCTAC AGGCAGATGC TCGCACCAGT 
GCCCGTGACG CAGCCCTCGT CTACCACAAG CTGCACCAGC TGCACACCAT GGGAAAGTAC 
TCAGGTGGGC ACCTCGCTGC TGCCAACCTG GCACTGAGTG CCCTGAACCT GGCCGAGTGC 
GCTGGAGATG CTGTGTCCGT GGCCACGCTG GCTGAGATCT ACGTGGCCGC CGCACTCAGG 
GTCAAGGCCA GTCTGCCCCG GGCCTTGCAT TTTCTGACAC GCTTCTTCCT GAGCAGTGCT 
CGCCAGGCCT GCCTGGCACA GAGCGGCTCA GTGCCCCTTG CCATGCAGTG GCTCTGCCAC 
CCTGTGGGCC ACCGTTTCTT CGTGGATGGC AACTGGGCCC TGTGCAGCGC CCCGAGGGAC 
AGCTTGTACA GCGTGGCTGG GAACCCAGTG GATCCCCTGG CCCAGGTGAC TCAACTGTTC 
CGCGAACATC TCTTGGAGCG AGCACTGAAT TGCGTGGCCC AGCCCAGCCC CAGTCCTGGA 
TCAGCCGAGG GGGACAAGGA GTTCTCAGAT GCCCTGGGGT ACCTGCAGCT GCTGAACAGC 
TGTTCGGACG TGGCCGGAGC TCCTACCTGC AGCTTCTCCA TCAGCTCCAG CATGGCTGCC 
ACCCCCGGCA CAGACCCGGT GGCCAAGTGG TGGGCCTCTC TGACAGCTGT GGTGACCCAC 
TGGCTTCGGC GGGATGAGGA GGCAGCCGAG AGGCTGTACC CATTGGTGGA GCACCTGCCC 
CGTGCCCTGC AGGAGTCCGA GAAACCCCTA CCCAGGGCGG CTCTGCACTC CTTCAAGGCT 
GCCCGGACCA TTCTAGGCCG CGGGAAGGCT GAGTCTGGCC CAGCCAGCCT GGCAATGTGT 
GAGAAGGCCA GTGGGTACCT GCAGGACAGC CTGGCCACCA CACCAGCTGA CAGCTCCATT 
GACAAGGCCT TGCAGCTGCT CCTGTGTGAC CTGCTCCTTG TGGCGCGCAC CAGCCTCTGG 
CAGCGGCAGA AGCTGCCGGC ACCCACCCAG GCCTCGCAGG GCCCTGGAGG TGGGGCCCAG 
GCCTCTGCCC TCGAGCTTCG TGGTTTCCAG AGGGACCTGA GTGGCCTGAG GCGTCTGGCA 
CAGAATGTTC GGCCTGCCAT GCGGAGGGTA TTCCTACATG AAGCCACTGC CCGACTGATG 
GCAGGGGCCA GCCCAGCGCG GACACACCAG CTTCTGGATC GCAGCCTGAG GAGGAGGGTC 
GGCCCCTGCA AAGGAGGTGC GGTGGCGGCG GAACTGGAGT CCCGGCCCAC GAGGCGGGAG 
CAGGCCGAGG CTTTGCTGCT GGCCTCTTGC TACCTGCCGC CCGGCTTCCT ATCGGCGCCC 
GGGCAGCGCG TGGGCATGCT GGCCGAGGCG GCGCGCACGC TGGAGAAGCT CGGTGACCGC 
CGGCTGCTGC ACGACTGTCA GCAGATGCTC ATGCGCCTGG GCGGTGGGAC CACTGTGACC 
TCCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836111.1
CDS	195..3743
Translation	MDEPPFNEAALELALAEPCELDAALLTDIEGASGPLDSAHGRRRAGGAEGPRATGPGRGTMDCTFEDMLQLINNQDSEFPGLFDPPYAGGGAGTTDPASPDASSPGSLSPPPSTMSSSLEGFLGVTKATPPPLSPPQPAPTPLKIYPSVPAFSPVPGIKEEPAPLTVLQPTTPQPLPGALLPQSVAATTPPQFSSAPIVGYPSPPGGFTGTPPGSSSQSLAGLPLASLPGVPPVSLHSQVQSAAPQQLLTATATPTVAPGATAVTSQIQQVPVLLQPHFIKADSLLLTTVKTDVGAPLKAAGIRSLGPGTAVQAAPLQTLVSGGAILATVPLVVDTDKLPINRLASGKAPGSAQSRGEKRTAHNAIEKRYRSSINDKIVELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLSLRTAVHKSKSLKDLVSACGSGGSTDVPMEGMKPEVVDTLSPPPSDAGSPSQSSPLSLGSRGSSSGGSGSDSEPDSPVFEDGQVNPEPLPAPHSQGMLDRSRLALCALVFLCLSCNPLASLLGSRGPAGPSDTTSINHGPRRSMLGAEGRDGPGWAPWLLPPLVWLMNGLLVLFSLALLFVYGEPVTRPHSCPAVHFWRHRKQADLDLARGDFAQAAQQLWLALRALGRPLPTSHLDLACSLLWSLIRHLLQRLWVGRWLAGWAGGLRRDRALQADARTSARDAALVYHKLHQLHTMGKYSGGHLAAANLALSALNLAECAGDAVSVATLAEIYVAAALRVKASLPRALHFLTRFFLSSARQACLAQSGSVPLAMQWLCHPVGHRFFVDGNWALCSAPRDSLYSVAGNPVDPLAQVTQLFREHLLERALNCVAQPSPSPGSAEGDKEFSDALGYLQLLNSCSDVAGAPTCSFSISSSMAATPGTDPVAKWWASLTAVVTHWLRRDEEAAERLYPLVEHLPRALQESEKPLPRAALHSFKAARTILGRGKAESGPASLAMCEKASGYLQDSLATTPADSSIDKALQLLLCDLLLVARTSLWQRQKLPAPTQASQGPGGGAQASALELRGFQRDLSGLRRLAQNVRPAMRRVFLHEATARLMAGASPARTHQLLDRSLRRRVGPCKGGAVAAELESRPTRREQAEALLLASCYLPPGFLSAPGQRVGMLAEAARTLEKLGDRRLLHDCQQMLMRLGGGTTVTSS

Target ORF information:

RefSeq Version	XM_024980343.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980343.1

ORF Insert Sequence:

ATGGACGAGC CACCCTTCAA CGAAGCGGCC TTGGAGCTGG CGCTGGCCGA GCCGTGCGAG 
CTGGACGCGG CGCTGCTGAC CGACATAGAA GGTGCGTCAG GGCCACTGGA CTCCGCGCAC 
GGGCGGCGCC GGGCCGGGGG CGCGGAGGGC CCACGGGCGA CGGGCCCGGG CCGCGGAACC 
ATGGATTGCA CGTTCGAAGA CATGCTTCAG CTCATCAACA ACCAAGACAG CGAATTCCCG 
GGCCTATTCG ACCCGCCCTA CGCTGGGGGC GGAGCAGGGA CCACAGACCC TGCCAGTCCT 
GACGCCAGCT CCCCGGGCAG CCTGTCCCCA CCTCCTTCCA CGATGAGCTC CTCACTTGAA 
GGCTTCCTGG GGGTGACCAA GGCGACACCC CCACCCTTGT CCCCTCCCCA GCCTGCGCCC 
ACCCCCCTGA AGATATACCC ATCTGTGCCT GCCTTCTCCC CGGTGCCTGG GATCAAGGAG 
GAGCCAGCGC CCCTGACTGT CCTCCAGCCC ACCACCCCCC AGCCCCTGCC TGGAGCTCTC 
CTGCCGCAGA GTGTTGCGGC CACCACCCCA CCGCAGTTCA GCTCTGCTCC CATTGTGGGC 
TACCCCAGCC CTCCGGGAGG CTTCACAGGG ACCCCTCCGG GGAGCAGCTC GCAGTCACTG 
GCTGGCCTGC CACTGGCTTC CCTGCCAGGG GTCCCGCCCG TCTCCTTGCA CAGCCAGGTT 
CAGAGCGCGG CCCCCCAGCA GCTGTTGACA GCCACAGCCA CCCCCACGGT GGCCCCTGGA 
GCAACCGCTG TGACCTCCCA GATACAGCAG GTCCCGGTCC TGCTGCAGCC CCACTTCATC 
AAGGCAGACT CGTTGCTCCT GACGACCGTG AAAACAGATG TGGGAGCCCC CTTGAAAGCG 
GCAGGCATCC GCTCCCTGGG CCCTGGCACT GCTGTTCAGG CAGCGCCCTT GCAGACCCTG 
GTGAGTGGCG GGGCCATCCT GGCCACGGTG CCACTGGTAG TAGACACTGA CAAGCTGCCC 
ATCAACCGAC TTGCCAGTGG CAAGGCTCCA GGCTCGGCGC AGAGCCGCGG CGAGAAGCGT 
ACAGCCCACA ACGCCATCGA GAAACGCTAC CGCTCTTCCA TCAATGACAA GATCGTTGAG 
CTCAAGGACC TGGTGGTGGG CACCGAGGCC AAGTTGAATA AATCTGCCGT CTTGCGCAAG 
GCCATCGACT ACATCCGCTT CCTTCAGCAC AGCAACCAGA AGCTCAAGCA GGAGAACCTG 
AGTCTGCGCA CTGCTGTCCA CAAAAGCAAA TCGCTGAAGG ACCTGGTGTC GGCTTGTGGC 
AGTGGAGGGA GCACAGATGT GCCCATGGAG GGCATGAAGC CAGAGGTGGT CGACACCCTG 
AGTCCACCCC CCTCAGATGC CGGCTCGCCC TCCCAGAGCA GCCCCTTGTC CCTTGGCAGC 
AGGGGCAGTA GCAGCGGTGG AAGTGGCAGT GACTCGGAGC CCGACAGCCC GGTCTTTGAG 
GACGGCCAGG TGAATCCAGA GCCGCTGCCT GCTCCCCACA GCCAGGGCAT GCTGGACCGC 
TCTCGCCTGG CCCTGTGCGC GCTCGTCTTC CTCTGTCTCT CCTGCAACCC CTTGGCCTCC 
CTGCTGGGTA GCCGGGGTCC TGCTGGCCCC TCCGACACCA CCAGCATCAA CCACGGTCCC 
AGGCGCAGCA TGCTGGGTGC TGAGGGCAGA GATGGTCCTG GCTGGGCCCC ATGGCTGCTG 
CCCCCACTGG TCTGGCTGAT GAATGGGCTG CTGGTGCTCT TCTCCTTGGC ACTTCTCTTT 
GTCTATGGAG AACCAGTCAC TCGGCCCCAC TCATGCCCCG CCGTGCACTT CTGGAGGCAT 
CGCAAGCAGG CTGACCTGGA CCTGGCCAGG GGGGACTTTG CCCAGGCTGC CCAGCAGCTG 
TGGCTGGCCC TGCGGGCCTT GGGCCGGCCT CTGCCCACCT CCCACCTGGA CCTGGCCTGC 
AGCCTGCTCT GGAGCCTCAT CCGCCACCTG CTGCAACGTC TCTGGGTGGG CCGCTGGCTG 
GCCGGCTGGG CAGGGGGCCT ACGGAGGGAC AGGGCCCTAC AGGCAGATGC TCGCACCAGT 
GCCCGTGACG CAGCCCTCGT CTACCACAAG CTGCACCAGC TGCACACCAT GGGAAAGTAC 
TCAGGTGGGC ACCTCGCTGC TGCCAACCTG GCACTGAGTG CCCTGAACCT GGCCGAGTGC 
GCTGGAGATG CTGTGTCCGT GGCCACGCTG GCTGAGATCT ACGTGGCCGC CGCACTCAGG 
GTCAAGGCCA GTCTGCCCCG GGCCTTGCAT TTTCTGACAC GCTTCTTCCT GAGCAGTGCT 
CGCCAGGCCT GCCTGGCACA GAGCGGCTCA GTGCCCCTTG CCATGCAGTG GCTCTGCCAC 
CCTGTGGGCC ACCGTTTCTT CGTGGATGGC AACTGGGCCC TGTGCAGCGC CCCGAGGGAC 
AGCTTGTACA GCGTGGCTGG GAACCCAGTG GATCCCCTGG CCCAGGTGAC TCAACTGTTC 
CGCGAACATC TCTTGGAGCG AGCACTGAAT TGCGTGGCCC AGCCCAGCCC CAGTCCTGGA 
TCAGCCGAGG GGGACAAGGA GTTCTCAGAT GCCCTGGGGT ACCTGCAGCT GCTGAACAGC 
TGTTCGGACG TGGCCGGAGC TCCTACCTGC AGCTTCTCCA TCAGCTCCAG CATGGCTGCC 
ACCCCCGGCA CAGACCCGGT GGCCAAGTGG TGGGCCTCTC TGACAGCTGT GGTGACCCAC 
TGGCTTCGGC GGGATGAGGA GGCAGCCGAG AGGCTGTACC CATTGGTGGA GCACCTGCCC 
CGTGCCCTGC AGGAGTCCGA GAAACCCCTA CCCAGGGCGG CTCTGCACTC CTTCAAGGCT 
GCCCGGACCA TTCTAGGCCG CGGGAAGGCT GAGTCTGGCC CAGCCAGCCT GGCAATGTGT 
GAGAAGGCCA GTGGGTACCT GCAGGACAGC CTGGCCACCA CACCAGCTGA CAGCTCCATT 
GACAAGGCCT TGCAGCTGCT CCTGTGTGAC CTGCTCCTTG TGGCGCGCAC CAGCCTCTGG 
CAGCGGCAGA AGCTGCCGGC ACCCACCCAG GCCTCGCAGG GCCCTGGAGG TGGGGCCCAG 
GCCTCTGCCC TCGAGCTTCG TGGTTTCCAG AGGGACCTGA GTGGCCTGAG GCGTCTGGCA 
CAGAATGTTC GGCCTGCCAT GCGGAGGGTA TTCCTACATG AAGCCACTGC CCGACTGATG 
GCAGGGGCCA GCCCAGCGCG GACACACCAG CTTCTGGATC GCAGCCTGAG GAGGAGGGTC 
GGCCCCTGCA AAGGAGGTGC GGTGGCGGCG GAACTGGAGT CCCGGCCCAC GAGGCGGGAG 
CAGGCCGAGG CTTTGCTGCT GGCCTCTTGC TACCTGCCGC CCGGCTTCCT ATCGGCGCCC 
GGGCAGCGCG TGGGCATGCT GGCCGAGGCG GCGCGCACGC TGGAGAAGCT CGGTGACCGC 
CGGCTGCTGC ACGACTGTCA GCAGATGCTC ATGCGCCTGG GCGGTGGGAC CACTGTGACC 
TCCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SREBF1 ( NM_001113302.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001106773.1 Bos taurus sterol regulatory element binding transcription factor 1 (SREBF1), mRNA.

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CloneID	OBa40072
Clone ID Related Accession (Same CDS sequence)	NM_001113302.1
Accession Version	NM_001113302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Bos taurus(cattle)
Product	sterol regulatory element-binding protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB355703.1. On Jan 10, 2008 this sequence version replaced XM_879234.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB355703.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGGACGAGC CACCCTTCAA CGAAGCGGCC TTGGAGCTGG CGCTGGCCGA GCCGTGCGAG 
CTGGACGCGG CGCTGCTGAC CGACATAGAA GACATGCTTC AGCTCATCAA CAACCAAGAC 
AGCGAATTCC CGGGCCTATT CGACCCGCCC TACGCTGGGG GCGGAGCAGG GACCACAGAC 
CCTGCCAGTC CTGACGCCAG CTCCCCGGGC AGCCTGTCCC CACCTCCTTC CACGATGAGC 
TCCTCACTTG AAGGCTTCCT GGGGGTGACC AAGGCGACAC CCCCACCCTT GTCCCCTCCC 
CAGCCTGCGC CCACCCCCCT GAAGATATAC CCATCTGTGC CTGCCTTCTC CCCGGTGCCT 
GGGATCAAGG AGGAGCCAGC GCCCCTGACT GTCCTCCAGC CCACCACCCC CCAGCCCCTG 
CCTGGAGCTC TCCTGCCGCA GAGTGTTGCG GCCACCACCC CACCGCAGTT CAGCTCTGCT 
CCCATTGTGG GCTACCCCAG CCCTCCGGGA GGCTTCACAG GGACCCCTCC GGGGAGCAGC 
TCGCAGTCAC TGGCTGGCCT GCCACTGGCT TCCCTGCCAG GGGTCCCGCC CGTCTCCTTG 
CACAGCCAGG TTCAGAGCGC GGCCCCCCAG CAGCTGTTGA CAGCCACAGC CACCCCCACG 
GTGGCCCCTG GAGCAACCGC TGTGACCTCC CAGATACAGC AGGTCCCGGT CCTGCTGCAG 
CCCCACTTCA TCAAGGCAGA CTCGTTGCTC CTGACGACCG TGAAAACAGA TGTGGGAGCC 
CCCTTGAAAG CGGCAGGCAT CCGCTCCCTG GGCCCTGGCA CTGCTGTTCA GGCAGCGCCC 
TTGCAGACCC TGGTGAGTGG CGGGGCCATC CTGGCCACGG TGCCACTGGT AGTAGACACT 
GACAAGCTGC CCATCAACCG ACTTGCCAGT GGCAAGGCTC CAGGCTCGGC GCAGAGCCGC 
GGCGAGAAGC GTACAGCCCA CAACGCCATC GAGAAACGCT ACCGCTCTTC CATCAATGAC 
AAGATCGTTG AGCTCAAGGA CCTGGTGGTG GGCACCGAGG CCAAGTTGAA TAAATCTGCC 
GTCTTGCGCA AGGCCATCGA CTACATCCGC TTCCTTCAGC ACAGCAACCA GAAGCTCAAG 
CAGGAGAACC TGAGTCTGCG CACTGCTGTC CACAAAAGCA AATCGCTGAA GGACCTGGTG 
TCGGCTTGTG GCAGTGGAGG GAGCACAGAT GTGCCCATGG AGGGCATGAA GCCAGAGGTG 
GTCGACACCC TGAGTCCACC CCCCTCAGAT GCCGGCTCGC CCTCCCAGAG CAGCCCCTTG 
TCCCTTGGCA GCAGGGGCAG TAGCAGCGGT GGAAGTGGCA GTGACTCGGA GCCCGACAGC 
CCGGTCTTTG AGGACGGCCA GGTGAATCCA GAGCCGCTGC CTGCTCCCCA CAGCCAGGGC 
ATGCTGGACC GCTCTCGCCT GGCCCTGTGC GCACTCGTCT TCCTCTGTCT CTCCTGCAAC 
CCCTTGGCCT CCCTGCTGGG TAGCCGGGGT CCTGCTGGCC CCTCCGACAC CACCAGCATC 
AACCACGGTC CCAGGCGCAG CATGCTGGGT GCTGAGGGCA GAGATGGTCC TGGCTGGGCC 
CCATGGCTGC TGCCCCCACT GGTCTGGCTG ATGAATGGGC TGCTGGTGCT CTTCTCCTTG 
GCACTTCTCT TTGTCTATGG AGAACCAGTC ACTCGGCCCC ACTCATGCCC CGCCGTGCAC 
TTCTGGAGGC ATCGCAAGCA GGCTGACCTG GACCTGGCCA GGGGGGACTT TGCCCAGGCT 
GCCCAGCAGC TGTGGCTGGC CCTGCGGGCC TTGGGCCGGC CTCTGCCCAC CTCCCACCTG 
GACCTGGCCT GCAGCCTGCT CTGGAGCCTC ATCCGCCACC TGCTGCAACG TCTCTGGGTG 
GGCCGCTGGC TGGCCGGCTG GGCAGGGGGC CTACGGAGGG ACAGGGCCCT ACAGGCAGAT 
GCTCGCACCA GTGCCCGTGA CGCAGCCCTC GTCTACCACA AGCTGCACCA GCTGCACACC 
ATGGGAAAGT ACTCAGGTGG GCACCTCGCT GCTGCCAACC TGGCACTGAG TGCCCTGAAC 
CTGGCCGAGT GCGCTGGAGA TGCTGTGTCC GTGGCCACGC TGGCTGAGAT CTACGTGGCC 
GCCGCACTCA GGGTCAAGGC CAGTCTGCCC CGGGCCTTGC ATTTTCTGAC ACGCTTCTTC 
CTGAGCAGTG CTCGCCAGGC CTGCCTGGCA CAGAGCGGCT CAGTGCCCCT TGCCATGCAG 
TGGCTCTGCC ACCCTGTGGG CCACCGTTTC TTCGTGGATG GCAACTGGGC CCTGTGCAGC 
GCCCCGAGGG ACAGCTTGTA CAGCGTGGCT GGGAACCCAG TGGATCCCCT GGCCCAGGTG 
ACTCAACTGT TCCGCGAACA TCTCTTGGAG CGAGCACTGA ATTGCGTGGC CCAGCCCAGC 
CCCAGTCCTG GATCAGCCGA GGGGGACAAG GAGTTCTCAG ATGCCCTGGG GTACCTGCAG 
CTGCTGAACA GCTGTTCGGA CGTGGCCGGA GCTCTTACCT GCAGCTTCTC CATCAGCTCC 
AGCATGGCTG CCACCCCCGG CACAGACCCG GTGGCCAAGT GGTGGGCCTC TCTGACAGCT 
GTGGTGACCC ACTGGCTTCG GCGGGATGAG GAGGCAGCCG AGAGGCTGTA CCCATTGGTG 
GAGCACCTGC CCCGTGCCCT GCAGGAGTCC GAGAAACCCC TACCCAGGGC GGCTCTGCAC 
TCCTTCAAGG CTGCCCGGAC CATTCTAGGC CGCGGGAAGG CTGAGTCTGG CCCAGCCAGC 
CTGGCAATGT GTGAGAAGGC CAGTGGGTAC CTGCAGGACA GCCTGGCCAC CACACCAGCT 
GACAGCTCCA TTGACAAGGC CTTGCAGCTG CTCCTGTGTG ACCTGCTCCT TGTGGCGCGC 
ACCAGCCTCT GGCAGCGGCA GAAGCTGCCG GCACCCACCC AGGCCTCGCA GGGCCCTGGA 
GGTGGGGCCC AGGCCTCTGC CCTCGAGCTT CGTGGTTTCC AGAGGGACCT GAGTGGCCTG 
AGGCGTCTGG CACAGAATGT TCGGCCTGCC ATGCGGAGGG TATTCCTACA TGAAGCCACT 
GCCCGACTGA TGGCAGGGGC CAGCCCAGCG CGGACACACC AGCTTCTGGA TCGCAGCCTG 
AGGAGGAGGG TCGGCCCCTG CAAAGGAGGT GCGGTGGCGG CGGAACTGGA GTCCCGGCCC 
ACGAGGCGGG AGCAGGCCGA GGCTTTGCTG CTGGCCTCTT GCTACCTGCC GCCCGGCTTC 
CTATCGGCGC CCGGGCAGCG CGTGGGCATG CTGGCCGAGG CGGCGCGCAC GCTGGAGAAG 
CTCGGTGACC GCCGGCTGCT GCACGACTGT CAGCAGATGC TCATGCGCCT GGGCGGTGGG 
ACCACTGTGA CCTCCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106773.1
CDS	50..3490
Translation	MDEPPFNEAALELALAEPCELDAALLTDIEDMLQLINNQDSEFPGLFDPPYAGGGAGTTDPASPDASSPGSLSPPPSTMSSSLEGFLGVTKATPPPLSPPQPAPTPLKIYPSVPAFSPVPGIKEEPAPLTVLQPTTPQPLPGALLPQSVAATTPPQFSSAPIVGYPSPPGGFTGTPPGSSSQSLAGLPLASLPGVPPVSLHSQVQSAAPQQLLTATATPTVAPGATAVTSQIQQVPVLLQPHFIKADSLLLTTVKTDVGAPLKAAGIRSLGPGTAVQAAPLQTLVSGGAILATVPLVVDTDKLPINRLASGKAPGSAQSRGEKRTAHNAIEKRYRSSINDKIVELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLSLRTAVHKSKSLKDLVSACGSGGSTDVPMEGMKPEVVDTLSPPPSDAGSPSQSSPLSLGSRGSSSGGSGSDSEPDSPVFEDGQVNPEPLPAPHSQGMLDRSRLALCALVFLCLSCNPLASLLGSRGPAGPSDTTSINHGPRRSMLGAEGRDGPGWAPWLLPPLVWLMNGLLVLFSLALLFVYGEPVTRPHSCPAVHFWRHRKQADLDLARGDFAQAAQQLWLALRALGRPLPTSHLDLACSLLWSLIRHLLQRLWVGRWLAGWAGGLRRDRALQADARTSARDAALVYHKLHQLHTMGKYSGGHLAAANLALSALNLAECAGDAVSVATLAEIYVAAALRVKASLPRALHFLTRFFLSSARQACLAQSGSVPLAMQWLCHPVGHRFFVDGNWALCSAPRDSLYSVAGNPVDPLAQVTQLFREHLLERALNCVAQPSPSPGSAEGDKEFSDALGYLQLLNSCSDVAGALTCSFSISSSMAATPGTDPVAKWWASLTAVVTHWLRRDEEAAERLYPLVEHLPRALQESEKPLPRAALHSFKAARTILGRGKAESGPASLAMCEKASGYLQDSLATTPADSSIDKALQLLLCDLLLVARTSLWQRQKLPAPTQASQGPGGGAQASALELRGFQRDLSGLRRLAQNVRPAMRRVFLHEATARLMAGASPARTHQLLDRSLRRRVGPCKGGAVAAELESRPTRREQAEALLLASCYLPPGFLSAPGQRVGMLAEAARTLEKLGDRRLLHDCQQMLMRLGGGTTVTSS

Target ORF information:

RefSeq Version	NM_001113302.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sterol regulatory element binding transcription factor 1 (SREBF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113302.1

ORF Insert Sequence:

ATGGACGAGC CACCCTTCAA CGAAGCGGCC TTGGAGCTGG CGCTGGCCGA GCCGTGCGAG 
CTGGACGCGG CGCTGCTGAC CGACATAGAA GACATGCTTC AGCTCATCAA CAACCAAGAC 
AGCGAATTCC CGGGCCTATT CGACCCGCCC TACGCTGGGG GCGGAGCAGG GACCACAGAC 
CCTGCCAGTC CTGACGCCAG CTCCCCGGGC AGCCTGTCCC CACCTCCTTC CACGATGAGC 
TCCTCACTTG AAGGCTTCCT GGGGGTGACC AAGGCGACAC CCCCACCCTT GTCCCCTCCC 
CAGCCTGCGC CCACCCCCCT GAAGATATAC CCATCTGTGC CTGCCTTCTC CCCGGTGCCT 
GGGATCAAGG AGGAGCCAGC GCCCCTGACT GTCCTCCAGC CCACCACCCC CCAGCCCCTG 
CCTGGAGCTC TCCTGCCGCA GAGTGTTGCG GCCACCACCC CACCGCAGTT CAGCTCTGCT 
CCCATTGTGG GCTACCCCAG CCCTCCGGGA GGCTTCACAG GGACCCCTCC GGGGAGCAGC 
TCGCAGTCAC TGGCTGGCCT GCCACTGGCT TCCCTGCCAG GGGTCCCGCC CGTCTCCTTG 
CACAGCCAGG TTCAGAGCGC GGCCCCCCAG CAGCTGTTGA CAGCCACAGC CACCCCCACG 
GTGGCCCCTG GAGCAACCGC TGTGACCTCC CAGATACAGC AGGTCCCGGT CCTGCTGCAG 
CCCCACTTCA TCAAGGCAGA CTCGTTGCTC CTGACGACCG TGAAAACAGA TGTGGGAGCC 
CCCTTGAAAG CGGCAGGCAT CCGCTCCCTG GGCCCTGGCA CTGCTGTTCA GGCAGCGCCC 
TTGCAGACCC TGGTGAGTGG CGGGGCCATC CTGGCCACGG TGCCACTGGT AGTAGACACT 
GACAAGCTGC CCATCAACCG ACTTGCCAGT GGCAAGGCTC CAGGCTCGGC GCAGAGCCGC 
GGCGAGAAGC GTACAGCCCA CAACGCCATC GAGAAACGCT ACCGCTCTTC CATCAATGAC 
AAGATCGTTG AGCTCAAGGA CCTGGTGGTG GGCACCGAGG CCAAGTTGAA TAAATCTGCC 
GTCTTGCGCA AGGCCATCGA CTACATCCGC TTCCTTCAGC ACAGCAACCA GAAGCTCAAG 
CAGGAGAACC TGAGTCTGCG CACTGCTGTC CACAAAAGCA AATCGCTGAA GGACCTGGTG 
TCGGCTTGTG GCAGTGGAGG GAGCACAGAT GTGCCCATGG AGGGCATGAA GCCAGAGGTG 
GTCGACACCC TGAGTCCACC CCCCTCAGAT GCCGGCTCGC CCTCCCAGAG CAGCCCCTTG 
TCCCTTGGCA GCAGGGGCAG TAGCAGCGGT GGAAGTGGCA GTGACTCGGA GCCCGACAGC 
CCGGTCTTTG AGGACGGCCA GGTGAATCCA GAGCCGCTGC CTGCTCCCCA CAGCCAGGGC 
ATGCTGGACC GCTCTCGCCT GGCCCTGTGC GCACTCGTCT TCCTCTGTCT CTCCTGCAAC 
CCCTTGGCCT CCCTGCTGGG TAGCCGGGGT CCTGCTGGCC CCTCCGACAC CACCAGCATC 
AACCACGGTC CCAGGCGCAG CATGCTGGGT GCTGAGGGCA GAGATGGTCC TGGCTGGGCC 
CCATGGCTGC TGCCCCCACT GGTCTGGCTG ATGAATGGGC TGCTGGTGCT CTTCTCCTTG 
GCACTTCTCT TTGTCTATGG AGAACCAGTC ACTCGGCCCC ACTCATGCCC CGCCGTGCAC 
TTCTGGAGGC ATCGCAAGCA GGCTGACCTG GACCTGGCCA GGGGGGACTT TGCCCAGGCT 
GCCCAGCAGC TGTGGCTGGC CCTGCGGGCC TTGGGCCGGC CTCTGCCCAC CTCCCACCTG 
GACCTGGCCT GCAGCCTGCT CTGGAGCCTC ATCCGCCACC TGCTGCAACG TCTCTGGGTG 
GGCCGCTGGC TGGCCGGCTG GGCAGGGGGC CTACGGAGGG ACAGGGCCCT ACAGGCAGAT 
GCTCGCACCA GTGCCCGTGA CGCAGCCCTC GTCTACCACA AGCTGCACCA GCTGCACACC 
ATGGGAAAGT ACTCAGGTGG GCACCTCGCT GCTGCCAACC TGGCACTGAG TGCCCTGAAC 
CTGGCCGAGT GCGCTGGAGA TGCTGTGTCC GTGGCCACGC TGGCTGAGAT CTACGTGGCC 
GCCGCACTCA GGGTCAAGGC CAGTCTGCCC CGGGCCTTGC ATTTTCTGAC ACGCTTCTTC 
CTGAGCAGTG CTCGCCAGGC CTGCCTGGCA CAGAGCGGCT CAGTGCCCCT TGCCATGCAG 
TGGCTCTGCC ACCCTGTGGG CCACCGTTTC TTCGTGGATG GCAACTGGGC CCTGTGCAGC 
GCCCCGAGGG ACAGCTTGTA CAGCGTGGCT GGGAACCCAG TGGATCCCCT GGCCCAGGTG 
ACTCAACTGT TCCGCGAACA TCTCTTGGAG CGAGCACTGA ATTGCGTGGC CCAGCCCAGC 
CCCAGTCCTG GATCAGCCGA GGGGGACAAG GAGTTCTCAG ATGCCCTGGG GTACCTGCAG 
CTGCTGAACA GCTGTTCGGA CGTGGCCGGA GCTCTTACCT GCAGCTTCTC CATCAGCTCC 
AGCATGGCTG CCACCCCCGG CACAGACCCG GTGGCCAAGT GGTGGGCCTC TCTGACAGCT 
GTGGTGACCC ACTGGCTTCG GCGGGATGAG GAGGCAGCCG AGAGGCTGTA CCCATTGGTG 
GAGCACCTGC CCCGTGCCCT GCAGGAGTCC GAGAAACCCC TACCCAGGGC GGCTCTGCAC 
TCCTTCAAGG CTGCCCGGAC CATTCTAGGC CGCGGGAAGG CTGAGTCTGG CCCAGCCAGC 
CTGGCAATGT GTGAGAAGGC CAGTGGGTAC CTGCAGGACA GCCTGGCCAC CACACCAGCT 
GACAGCTCCA TTGACAAGGC CTTGCAGCTG CTCCTGTGTG ACCTGCTCCT TGTGGCGCGC 
ACCAGCCTCT GGCAGCGGCA GAAGCTGCCG GCACCCACCC AGGCCTCGCA GGGCCCTGGA 
GGTGGGGCCC AGGCCTCTGC CCTCGAGCTT CGTGGTTTCC AGAGGGACCT GAGTGGCCTG 
AGGCGTCTGG CACAGAATGT TCGGCCTGCC ATGCGGAGGG TATTCCTACA TGAAGCCACT 
GCCCGACTGA TGGCAGGGGC CAGCCCAGCG CGGACACACC AGCTTCTGGA TCGCAGCCTG 
AGGAGGAGGG TCGGCCCCTG CAAAGGAGGT GCGGTGGCGG CGGAACTGGA GTCCCGGCCC 
ACGAGGCGGG AGCAGGCCGA GGCTTTGCTG CTGGCCTCTT GCTACCTGCC GCCCGGCTTC 
CTATCGGCGC CCGGGCAGCG CGTGGGCATG CTGGCCGAGG CGGCGCGCAC GCTGGAGAAG 
CTCGGTGACC GCCGGCTGCT GCACGACTGT CAGCAGATGC TCATGCGCCT GGGCGGTGGG 
ACCACTGTGA CCTCCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Epigenetic mechanisms contribute to decrease stearoyl-CoA desaturase 1 expression in the liver of dairy cows after prolonged feeding of high-concentrate diet.
Journal of dairy science(2017 Dec)
Xu TL,Seyfert HM,Shen XZ

Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression.
Research in veterinary science10716-9(2016 Aug)
Prodanovi? R,Kori?anac G,Vujanac I,Djordjevi? A,Panteli? M,Romi? S,Stanimirovi? Z,Kirovski D

Stearoyl-CoA desaturase 1 expression is downregulated in liver and udder during E. coli mastitis through enhanced expression of repressive C/EBP factors and reduced expression of the inducer SREBP1A.
BMC molecular biology17(1)16(2016 07)
Xu T,Shen X,Seyfert HM

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SREBF1 cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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