GPT cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPT
Entrez Gene ID	539188
Full Name	glutamic--pyruvic transaminase
General protein information	Preferred Names glutamic--pyruvic transaminase Names alanine aminotransferase 1 ALT1 GPT 1 glutamate pyruvate transaminase 1 glutamic--alanine transaminase 1 glutamic--pyruvic transaminase 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015474356.1	XP_015329842.1	alanine aminotransferase 1 isoform X1
XM_015474358.1	XP_015329844.1	alanine aminotransferase 1 isoform X3
XM_015474357.1	XP_015329843.1	alanine aminotransferase 1 isoform X2
XM_015474359.1	XP_015329845.1	alanine aminotransferase 1 isoform X4
XM_015474360.1	XP_015329846.1	alanine aminotransferase 1 isoform X5
XM_010811860.2	XP_010810162.1	alanine aminotransferase 1 isoform X6
NM_001083740.2	NP_001077209.1	alanine aminotransferase 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:ch211-217a12.1	XP_001919896.1
Saccharomyces cerevisiae (baker's yeast)	ALT2	NP_010396.1
Arabidopsis thaliana (thale cress)	AOAT2	NP_974122.1
Homo sapiens (human)	GPT	NP_005300.1
	GPT	XP_001093616.1
Bos taurus (cattle)	GPT	NP_001077209.1
Rattus norvegicus (Norway rat)	Gpt	NP_112301.1
Danio rerio (zebrafish)	gpt2l	NP_001136246.1
Arabidopsis thaliana (thale cress)	GGT1	NP_564192.2
Canis lupus familiaris (dog)	GPT	XP_005628374.1
Mus musculus (house mouse)	Gpt	NP_877957.1
Pan troglodytes (chimpanzee)	GPT	XP_003951417.1
Eremothecium gossypii	AGOS_AGR085W	NP_986750.1
Oryza sativa (rice)	Os07g0108300	NP_001058716.1
Xenopus tropicalis (tropical clawed frog)	gpt2	NP_001016805.1

Gene Ontology
Bibliography

Related articles in PubMed

Glutamine synthetase and alanine transaminase expression are decreased in livers of aged vs. young beef cows and GS can be upregulated by 17?-estradiol implants.
Miles ED, McBride BW, Jia Y, Liao SF, Boling JA, Bridges PJ, Matthews JC
Journal of animal science93(9)4500-9(2015 Sep)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Assessment of the gene content of the chromosomal regions flanking bovine DGAT1.
Winter A, Alzinger A, Fries R
Genomics83(1)172-80(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study concludes that hepatic expression of alanine transaminase and glutathione synthetase (GS) are reduced in aged cows, and administration of 17beta-estradiol increases plasma estradiol and hepatic GS.
Title: Glutamine synthetase and alanine transaminase expression are decreased in livers of aged vs. young beef cows and GS can be upregulated by 17?-estradiol implants.

The following GPT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa00753	NM_001083740.2 Latest version!	Bos taurus glutamic--pyruvic transaminase (GPT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa54219	XM_010811860.2 Latest version!	Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa92454	XM_015474356.1 Latest version!	Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa92455	XM_015474357.1 Latest version!	Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa92456	XM_015474358.1 Latest version!	Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa92457	XM_015474359.1 Latest version!	Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa92458	XM_015474360.1 Latest version!	Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPT ( NM_001083740.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001077209.1 Bos taurus glutamic--pyruvic transaminase (GPT), mRNA.

Price & Availability↑

CloneID	OBa00753
Clone ID Related Accession (Same CDS sequence)	NM_001083740.2
Accession Version	NM_001083740.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	alanine aminotransferase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134583.1. On Aug 31, 2012 this sequence version replaced NM_001083740.1. ##Evidence-Data-START## Transcript exon combination :: BC134583.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTTGA GGGCAGGTGA GCACAGCCAG GAGGCAGCAA ATGGGCTGAA GGAGAAGGTG 
CTGACACTGG ACTCCATGAA TCCTTATGTC CGGAGGGTGG AGTATGCGGT GCGAGGCCCC 
ATCGTTCAGC GGGCACTGGA GCTGGAGCAG GAGCTGCGCC AGGGCGTAAA GAAGCCCTTC 
ACCGAGGTCA TCCGCGCCAA CATCGGGGAC GCACAGGCCA TGGGGCAGAT CCCTATCACC 
TTCCCGCGCC AGGTCCTGGC CCTCTGCGTC CATCCCGATC TCCTGAACAG CCCCGACTTC 
CCCGACGACG CCAAGAGGAG GGCGGAGCGC ATCTTGCAGG CGTGTGGGGG CCACAGCCTG 
GGGGCCTACA GCATCAGCGC TGGCGTCCAG ATGATCCGTG AGGATGTGGC GCGGTACATC 
GAGCGGCGCG ATGGAGGCAT TCCTGCCGAC CCCAATAACA TCTTTCTGTC CACGGGGGCC 
AGCGATGCCA TTGTGACGGT GCTGAAGTTG CTGGTAACCG GCGAGGGTCG CACGCGCACG 
GGCGTGCTTA TCCCTATCCC TCAGTATCCA CTCTACTCCG CCGCGCTGGC CGAGTTCAAT 
GCGGTGCAGG TGGACTACTA CCTGGACGAG GAGCGTGCCT GGGCGCTCGA TGTGGCCGAG 
CTGCGGCGCG CGCTGCGCCA GGCGCGTGAC CACTGCCGCC CCCGCGCGCT CTGCGTCATC 
AACCCCGGGA ACCCCACCGG GCAGGTGCAG ACCCGCGAGT GCATCGAGGA CGTGATCCGC 
TTCGCCTATG AGGAGAAGCT TTTCCTATTG GCCGATGAGG TGTACCAAGA CAACGTGTAT 
GCCGAGAGCT CGCAGTTCCA CTCGTTCAAG AAGGTGCTCA CGGAGATGGG GCCGCCGTAC 
GCGGCGCAAC AGGAGCTCGC CTCCTTCCAC TCGATCTCCA AGGGCTACAT GGGCGAGTGC 
GGCTTCCGCG GCGGCTACGT GGAGGTGGTG AATATGGACG CTGCGGTGAA GCAGCAGATG 
CAGAAGCTGC GGAGCGTGCG GCTGTGCCCA CCCACCCCGG GCCAGGTCCT GCTCGACGTG 
GCTGTCAGCC CGCCGGCGCC CTCAGACCCC TCCTTCCCGC GGTTCCAGGC GGAGAGGAGG 
GCGGTGCTGG CCGAGCTGGC TGCCAAGGCC AAGCTCACGG AGCAGGTCTT CAACGAAGCT 
CCCGGCATCC GCTGCAATCC GGTGCAGGGC GCCATGTATT CGTTCCCGCG CGTGCAGCTG 
CCCCCGCGTG CGGTGCAGCG CGCTCAGGAG CTGGGCCTGG CTCCCGACAT GTTCTTCTGC 
CTGCGCCTCC TAGAGGAGAC TGGCATCTGC GTGGTGCCTG GGAGTGGCTT TGGACAACGG 
GAAGGCACCT ACCACTTTCG GATGACTATT CTGCCCCCCA TGGAGAAGCT ACGGCCCCTG 
CTGGAGAAGC TGAGCCAGTT CCATGCGAAG TTCACCCGCG AGTACTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077209.1
CDS	149..1639
Translation	MALRAGEHSQEAANGLKEKVLTLDSMNPYVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQIPITFPRQVLALCVHPDLLNSPDFPDDAKRRAERILQACGGHSLGAYSISAGVQMIREDVARYIERRDGGIPADPNNIFLSTGASDAIVTVLKLLVTGEGRTRTGVLIPIPQYPLYSAALAEFNAVQVDYYLDEERAWALDVAELRRALRQARDHCRPRALCVINPGNPTGQVQTRECIEDVIRFAYEEKLFLLADEVYQDNVYAESSQFHSFKKVLTEMGPPYAAQQELASFHSISKGYMGECGFRGGYVEVVNMDAAVKQQMQKLRSVRLCPPTPGQVLLDVAVSPPAPSDPSFPRFQAERRAVLAELAAKAKLTEQVFNEAPGIRCNPVQGAMYSFPRVQLPPRAVQRAQELGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPMEKLRPLLEKLSQFHAKFTREYS

Target ORF information:

RefSeq Version	NM_001083740.2
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamic--pyruvic transaminase (GPT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083740.2

ORF Insert Sequence:

ATGGCCTTGA GGGCAGGTGA GCACAGCCAG GAGGCAGCAA ATGGGCTGAA GGAGAAGGTG 
CTGACACTGG ACTCCATGAA TCCTTATGTC CGGAGGGTGG AGTATGCGGT GCGAGGCCCC 
ATCGTTCAGC GGGCACTGGA GCTGGAGCAG GAGCTGCGCC AGGGCGTAAA GAAGCCCTTC 
ACCGAGGTCA TCCGCGCCAA CATCGGGGAC GCACAGGCCA TGGGGCAGAT CCCTATCACC 
TTCCCGCGCC AGGTCCTGGC CCTCTGCGTC CATCCCGATC TCCTGAACAG CCCCGACTTC 
CCCGACGACG CCAAGAGGAG GGCGGAGCGC ATCTTGCAGG CGTGTGGGGG CCACAGCCTG 
GGGGCCTACA GCATCAGCGC TGGCGTCCAG ATGATCCGTG AGGATGTGGC GCGGTACATC 
GAGCGGCGCG ATGGAGGCAT TCCTGCCGAC CCCAATAACA TCTTTCTGTC CACGGGGGCC 
AGCGATGCCA TTGTGACGGT GCTGAAGTTG CTGGTAACCG GCGAGGGTCG CACGCGCACG 
GGCGTGCTTA TCCCTATCCC TCAGTATCCA CTCTACTCCG CCGCGCTGGC CGAGTTCAAT 
GCGGTGCAGG TGGACTACTA CCTGGACGAG GAGCGTGCCT GGGCGCTCGA TGTGGCCGAG 
CTGCGGCGCG CGCTGCGCCA GGCGCGTGAC CACTGCCGCC CCCGCGCGCT CTGCGTCATC 
AACCCCGGGA ACCCCACCGG GCAGGTGCAG ACCCGCGAGT GCATCGAGGA CGTGATCCGC 
TTCGCCTATG AGGAGAAGCT TTTCCTATTG GCCGATGAGG TGTACCAAGA CAACGTGTAT 
GCCGAGAGCT CGCAGTTCCA CTCGTTCAAG AAGGTGCTCA CGGAGATGGG GCCGCCGTAC 
GCGGCGCAAC AGGAGCTCGC CTCCTTCCAC TCGATCTCCA AGGGCTACAT GGGCGAGTGC 
GGCTTCCGCG GCGGCTACGT GGAGGTGGTG AATATGGACG CTGCGGTGAA GCAGCAGATG 
CAGAAGCTGC GGAGCGTGCG GCTGTGCCCA CCCACCCCGG GCCAGGTCCT GCTCGACGTG 
GCTGTCAGCC CGCCGGCGCC CTCAGACCCC TCCTTCCCGC GGTTCCAGGC GGAGAGGAGG 
GCGGTGCTGG CCGAGCTGGC TGCCAAGGCC AAGCTCACGG AGCAGGTCTT CAACGAAGCT 
CCCGGCATCC GCTGCAATCC GGTGCAGGGC GCCATGTATT CGTTCCCGCG CGTGCAGCTG 
CCCCCGCGTG CGGTGCAGCG CGCTCAGGAG CTGGGCCTGG CTCCCGACAT GTTCTTCTGC 
CTGCGCCTCC TAGAGGAGAC TGGCATCTGC GTGGTGCCTG GGAGTGGCTT TGGACAACGG 
GAAGGCACCT ACCACTTTCG GATGACTATT CTGCCCCCCA TGGAGAAGCT ACGGCCCCTG 
CTGGAGAAGC TGAGCCAGTT CCATGCGAAG TTCACCCGCG AGTACTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPT ( XM_010811860.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810162.1 Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa54219
Clone ID Related Accession (Same CDS sequence)	XM_010811860.2
Accession Version	XM_010811860.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	alanine aminotransferase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811860.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCTA TCGCTCCCTG CAGGGCCTGC AGAGGTGGGC CCCGAGGCTT ATCACAGCTC 
CCTTCCCACT CTTTGCACCA ATCAGGAGCC TCAGGGAGGG GGCACTTAGG TCACCTTGCC 
CCTGGTGCAG CCCCAAGGGC AGCTCCACGC GGTGCAGCCC CAAGGGCAGC TCCAGGCTGG 
TACGTGTTTG GCAAAAGGGC AGGACAGCAG CCCAGAGCTG CAGAGGTGTC CCGAGGGCCC 
TGCGAAGGCC CGGAGCTGCC CAGCACCCCC ACTCCCAGCA CCCCAGCTCA GAGACTCCAG 
CCTTGCAGCA CCGATGGGGG ACCAAGCTCC AGACACAGGC TCTTGCTGGC CCAGGCCTGG 
CCCCAGCGCA GGAGGGCCTC GCTCCCCTCC CCCAACAGCC CCAAGATGCC TGAGGCCTGG 
CTCAGTTACC CGGGAGACAG GCCTGCTCTG CTGGCCGGGA GCAAAGATGG GGGGTCCGAG 
AGGGCTGGGC CTCGGCCGAG CCCTACTGGG GTAACTATGC TGAGGTCCTG GGCCTTAGTC 
CCGCTCGCTT GGGGGCCCAG CCCTCCCAGC TCTGTGGACC CCTCAGGGCG CTCACCCCCT 
CCCTGCCCGC AGGCCTGGTC CCACAGAGCT GCTGGCACCT TCTGCCTGTC CCTGCCCCAT 
GTGGGTCTCC TGTCCCCTGT CCTGGGGACG CTGGGCGGGC ACCGGGCCTC ACCCCAAGCC 
CCGCCTGCCT TCTCACCCAC TCTCTGTGGC TCCCAGGGCA GATCTGTGCC CAGGTGGGCG 
GGGCCTGGCA GTCCGGCTCC TTCAGCCTCT TCTGTCCTCT CAGTGGGGCC AGCAGGCTGC 
AGCCAAGAGG GCGCCCCTTC CCAGGCTCCC CCTTCCCAGC CCTGGCCCAT CGAGCCTGGA 
CCGGCTGACC TCACCCCTGC TTCTGGATCC TGCTGCCTCC TGAGCCTCCT TCCTGCCCAT 
CTGAGCAGAG TCATGGCCTT GAGGGCAGGT GAGCACAGCC AGGAGGCAGC AAATGGGCTG 
AAGGAGAAGG TGCTGACACT GGACTCCATG AATCCTTATG TCCGGAGGGT GGAGTATGCG 
GTGCGAGGCC CCATCGTTCA GCGGGCACTG GAGCTGGAGC AGGAGCTGCG CCAGGGCGTA 
AAGAAGCCCT TCACCGAGGT CATCCGCGCC AACATCGGGG ACGCACAGGC CATGGGGCAG 
ATCCCTATCA CCTTCCCGCG CCAGGTCCTG GCCCTCTGCG TCCATCCCGA TCTCCTGAAC 
AGCCCCGACT TCCCCGACGA CGCCAAGAGG AGGGCGGAGC GCATCTTGCA GGCGTGTGGG 
GGCCACAGCC TGGGGGCCTA CAGCATCAGC GCTGGCGTCC AGATGATCCG TGAGGATGTG 
GCGCGGTACA TCGAGCGGCG CGATGGAGGC ATTCCTGCCG ACCCCAATAA CATCTTTCTG 
TCCACGGGGG CCAGCGATGC CATTGTGACG GTGCTGAAGT TGCTGGTAAC CGGCGAGGGT 
CGCACGCGCA CGGGCGTGCT TATCCCTATC CCTCAGTATC CACTCTACTC CGCCGCGCTG 
GCCGAGTTCA ATGCGGTGCA GGTGGACTAC TACCTGGACG AGGAGCGTGC CTGGGCGCTC 
GATGTGGCCG AGCTGCGGCG CGCGCTGCGC CAGGCGCGTG ACCACTGCCG CCCCCGCGCG 
CTCTGCGTCA TCAACCCCGG GAACCCCACC GGGCAGGTGC AGACCCGCGA GTGCATCGAG 
GACGTGATCC GCTTCGCCTA TGAGGAGAAG CTTTTCCTAT TGGCCGATGA GGTGTACCAA 
GACAACGTGT ATGCCGAGAG CTCGCAGTTC CACTCGTTCA AGAAGGTGCT CACGGAGATG 
GGGCCGCCGT ACGCGGCGCA ACAGGAGCTC GCCTCCTTCC ACTCGATCTC CAAGGGCTAC 
ATGGGCGAGT GCGGCTTCCG CGGCGGCTAC GTGGAGGTGG TGAATATGGA CGCTGCGGTG 
AAGCAGCAGA TGCAGAAGCT GCGGAGCGTG CGGCTGTGCC CACCCACCCC GGGCCAGGTC 
CTGCTCGACG TGGCTGTCAG CCCGCCGGCG CCCTCAGACC CCTCCTTCCC GCGGTTCCAG 
GCGGAGCTGG GCCTGGCTCC CGACATGTTC TTCTGCCTGC GCCTCCTAGA GGAGACTGGC 
ATCTGCGTGG TGCCTGGGAG TGGCTTTGGA CAACGGGAAG GCACCTACCA CTTTCGGATG 
ACTATTCTGC CCCCCATGGA GAAGCTACGG CCCCTGCTGG AGAAGCTGAG CCAGTTCCAT 
GCGAAGTTCA CCCGCGAGTA CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810162.1
CDS	980..3286
Translation	MGPIAPCRACRGGPRGLSQLPSHSLHQSGASGRGHLGHLAPGAAPRAAPRGAAPRAAPGWYVFGKRAGQQPRAAEVSRGPCEGPELPSTPTPSTPAQRLQPCSTDGGPSSRHRLLLAQAWPQRRRASLPSPNSPKMPEAWLSYPGDRPALLAGSKDGGSERAGPRPSPTGVTMLRSWALVPLAWGPSPPSSVDPSGRSPPPCPQAWSHRAAGTFCLSLPHVGLLSPVLGTLGGHRASPQAPPAFSPTLCGSQGRSVPRWAGPGSPAPSASSVLSVGPAGCSQEGAPSQAPPSQPWPIEPGPADLTPASGSCCLLSLLPAHLSRVMALRAGEHSQEAANGLKEKVLTLDSMNPYVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQIPITFPRQVLALCVHPDLLNSPDFPDDAKRRAERILQACGGHSLGAYSISAGVQMIREDVARYIERRDGGIPADPNNIFLSTGASDAIVTVLKLLVTGEGRTRTGVLIPIPQYPLYSAALAEFNAVQVDYYLDEERAWALDVAELRRALRQARDHCRPRALCVINPGNPTGQVQTRECIEDVIRFAYEEKLFLLADEVYQDNVYAESSQFHSFKKVLTEMGPPYAAQQELASFHSISKGYMGECGFRGGYVEVVNMDAAVKQQMQKLRSVRLCPPTPGQVLLDVAVSPPAPSDPSFPRFQAELGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPMEKLRPLLEKLSQFHAKFTREYS

Target ORF information:

RefSeq Version	XM_010811860.2
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811860.2

ORF Insert Sequence:

ATGGGGCCTA TCGCTCCCTG CAGGGCCTGC AGAGGTGGGC CCCGAGGCTT ATCACAGCTC 
CCTTCCCACT CTTTGCACCA ATCAGGAGCC TCAGGGAGGG GGCACTTAGG TCACCTTGCC 
CCTGGTGCAG CCCCAAGGGC AGCTCCACGC GGTGCAGCCC CAAGGGCAGC TCCAGGCTGG 
TACGTGTTTG GCAAAAGGGC AGGACAGCAG CCCAGAGCTG CAGAGGTGTC CCGAGGGCCC 
TGCGAAGGCC CGGAGCTGCC CAGCACCCCC ACTCCCAGCA CCCCAGCTCA GAGACTCCAG 
CCTTGCAGCA CCGATGGGGG ACCAAGCTCC AGACACAGGC TCTTGCTGGC CCAGGCCTGG 
CCCCAGCGCA GGAGGGCCTC GCTCCCCTCC CCCAACAGCC CCAAGATGCC TGAGGCCTGG 
CTCAGTTACC CGGGAGACAG GCCTGCTCTG CTGGCCGGGA GCAAAGATGG GGGGTCCGAG 
AGGGCTGGGC CTCGGCCGAG CCCTACTGGG GTAACTATGC TGAGGTCCTG GGCCTTAGTC 
CCGCTCGCTT GGGGGCCCAG CCCTCCCAGC TCTGTGGACC CCTCAGGGCG CTCACCCCCT 
CCCTGCCCGC AGGCCTGGTC CCACAGAGCT GCTGGCACCT TCTGCCTGTC CCTGCCCCAT 
GTGGGTCTCC TGTCCCCTGT CCTGGGGACG CTGGGCGGGC ACCGGGCCTC ACCCCAAGCC 
CCGCCTGCCT TCTCACCCAC TCTCTGTGGC TCCCAGGGCA GATCTGTGCC CAGGTGGGCG 
GGGCCTGGCA GTCCGGCTCC TTCAGCCTCT TCTGTCCTCT CAGTGGGGCC AGCAGGCTGC 
AGCCAAGAGG GCGCCCCTTC CCAGGCTCCC CCTTCCCAGC CCTGGCCCAT CGAGCCTGGA 
CCGGCTGACC TCACCCCTGC TTCTGGATCC TGCTGCCTCC TGAGCCTCCT TCCTGCCCAT 
CTGAGCAGAG TCATGGCCTT GAGGGCAGGT GAGCACAGCC AGGAGGCAGC AAATGGGCTG 
AAGGAGAAGG TGCTGACACT GGACTCCATG AATCCTTATG TCCGGAGGGT GGAGTATGCG 
GTGCGAGGCC CCATCGTTCA GCGGGCACTG GAGCTGGAGC AGGAGCTGCG CCAGGGCGTA 
AAGAAGCCCT TCACCGAGGT CATCCGCGCC AACATCGGGG ACGCACAGGC CATGGGGCAG 
ATCCCTATCA CCTTCCCGCG CCAGGTCCTG GCCCTCTGCG TCCATCCCGA TCTCCTGAAC 
AGCCCCGACT TCCCCGACGA CGCCAAGAGG AGGGCGGAGC GCATCTTGCA GGCGTGTGGG 
GGCCACAGCC TGGGGGCCTA CAGCATCAGC GCTGGCGTCC AGATGATCCG TGAGGATGTG 
GCGCGGTACA TCGAGCGGCG CGATGGAGGC ATTCCTGCCG ACCCCAATAA CATCTTTCTG 
TCCACGGGGG CCAGCGATGC CATTGTGACG GTGCTGAAGT TGCTGGTAAC CGGCGAGGGT 
CGCACGCGCA CGGGCGTGCT TATCCCTATC CCTCAGTATC CACTCTACTC CGCCGCGCTG 
GCCGAGTTCA ATGCGGTGCA GGTGGACTAC TACCTGGACG AGGAGCGTGC CTGGGCGCTC 
GATGTGGCCG AGCTGCGGCG CGCGCTGCGC CAGGCGCGTG ACCACTGCCG CCCCCGCGCG 
CTCTGCGTCA TCAACCCCGG GAACCCCACC GGGCAGGTGC AGACCCGCGA GTGCATCGAG 
GACGTGATCC GCTTCGCCTA TGAGGAGAAG CTTTTCCTAT TGGCCGATGA GGTGTACCAA 
GACAACGTGT ATGCCGAGAG CTCGCAGTTC CACTCGTTCA AGAAGGTGCT CACGGAGATG 
GGGCCGCCGT ACGCGGCGCA ACAGGAGCTC GCCTCCTTCC ACTCGATCTC CAAGGGCTAC 
ATGGGCGAGT GCGGCTTCCG CGGCGGCTAC GTGGAGGTGG TGAATATGGA CGCTGCGGTG 
AAGCAGCAGA TGCAGAAGCT GCGGAGCGTG CGGCTGTGCC CACCCACCCC GGGCCAGGTC 
CTGCTCGACG TGGCTGTCAG CCCGCCGGCG CCCTCAGACC CCTCCTTCCC GCGGTTCCAG 
GCGGAGCTGG GCCTGGCTCC CGACATGTTC TTCTGCCTGC GCCTCCTAGA GGAGACTGGC 
ATCTGCGTGG TGCCTGGGAG TGGCTTTGGA CAACGGGAAG GCACCTACCA CTTTCGGATG 
ACTATTCTGC CCCCCATGGA GAAGCTACGG CCCCTGCTGG AGAAGCTGAG CCAGTTCCAT 
GCGAAGTTCA CCCGCGAGTA CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPT ( XM_015474356.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329842.1 Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa92454
Clone ID Related Accession (Same CDS sequence)	XM_015474356.1
Accession Version	XM_015474356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	alanine aminotransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCTA TCGCTCCCTG CAGGGCCTGC AGAGGTGGGC CCCGAGGCTT ATCACAGCTC 
CCTTCCCACT CTTTGCACCA ATCAGGAGCC TCAGGGAGGG GGCACTTAGG TCACCTTGCC 
CCTGGTGCAG CCCCAAGGGC AGCTCCACGC GGTGCAGCCC CAAGGGCAGC TCCAGGCTGG 
TACGTGTTTG GCAAAAGGGC AGGACAGCAG CCCAGAGCTG CAGAGGTGTC CCGAGGGCCC 
TGCGAAGGCC CGGAGCTGCC CAGCACCCCC ACTCCCAGCA CCCCAGCTCA GAGACTCCAG 
CCTTGCAGCA CCGATGGGGG ACCAAGCTCC AGACACAGGC TCTTGCTGGC CCAGGCCTGG 
CCCCAGCGCA GGAGGGCCTC GCTCCCCTCC CCCAACAGCC CCAAGATGCC TGAGGCCTGG 
CTCAGTTACC CGGGAGACAG GCCTGCTCTG CTGGCCGGGA GCAAAGATGG GGGGTCCGAG 
AGGGCTGGGC CTCGGCCGAG CCCTACTGGG GTAACTATGC TGAGGTCCTG GGCCTTAGTC 
CCGCTCGCTT GGGGGCCCAG CCCTCCCAGC TCTGTGGACC CCTCAGGGCG CTCACCCCCT 
CCCTGCCCGC AGGCCTGGTC CCACAGAGCT GCTGGCACCT TCTGCCTGTC CCTGCCCCAT 
GTGGGTCTCC TGTCCCCTGT CCTGGGGACG CTGGGCGGGC ACCGGGCCTC ACCCCAAGCC 
CCGCCTGCCT TCTCACCCAC TCTCTGTGGC TCCCAGGGCA GATCTGTGCC CAGGTGGGCG 
GGGCCTGGCA GTCCGGCTCC TTCAGCCTCT TCTGTCCTCT CAGTGGGGCC AGCAGGCTGC 
AGCCAAGAGG GCGCCCCTTC CCAGGCTCCC CCTTCCCAGC CCTGGCCCAT CGAGCCTGGA 
CCGGCTGACC TCACCCCTGC TTCTGGATCC TGCTGCCTCC TGAGCCTCCT TCCTGCCCAT 
CTGAGCAGAG TCATGGCCTT GAGGGCAGGT GAGCACAGCC AGGAGGCAGC AAATGGGCTG 
AAGGAGAAGG TGCTGACACT GGACTCCATG AATCCTTATG TCCGGAGGGT GGAGTATGCG 
GTGCGAGGCC CCATCGTTCA GCGGGCACTG GAGCTGGAGC AGGAGCTGCG CCAGGGCGTA 
AAGAAGCCCT TCACCGAGGT CATCCGCGCC AACATCGGGG ACGCACAGGC CATGGGGCAG 
ATCCCTATCA CCTTCCCGCG CCAGGTCCTG GCCCTCTGCG TCCATCCCGA TCTCCTGAAC 
AGCCCCGACT TCCCCGACGA CGCCAAGAGG AGGGCGGAGC GCATCTTGCA GGCGTGTGGG 
GGCCACAGCC TGGGGGCCTA CAGCATCAGC GCTGGCGTCC AGATGATCCG TGAGGATGTG 
GCGCGGTACA TCGAGCGGCG CGATGGAGGC ATTCCTGCCG ACCCCAATAA CATCTTTCTG 
TCCACGGGGG CCAGCGATGC CATTGTGACG GTGCTGAAGT TGCTGGTAAC CGGCGAGGGT 
CGCACGCGCA CGGGCGTGCT TATCCCTATC CCTCAGTATC CACTCTACTC CGCCGCGCTG 
GCCGAGTTCA ATGCGGTGCA GGTGGACTAC TACCTGGACG AGGAGCGTGC CTGGGCGCTC 
GATGTGGCCG AGCTGCGGCG CGCGCTGCGC CAGGCGCGTG ACCACTGCCG CCCCCGCGCG 
CTCTGCGTCA TCAACCCCGG GAACCCCACC GGGCAGGTGC AGACCCGCGA GTGCATCGAG 
GACGTGATCC GCTTCGCCTA TGAGGAGAAG CTTTTCCTAT TGGCCGATGA GGTGTACCAA 
GACAACGTGT ATGCCGAGAG CTCGCAGTTC CACTCGTTCA AGAAGGTGCT CACGGAGATG 
GGGCCGCCGT ACGCGGCGCA ACAGGAGCTC GCCTCCTTCC ACTCGATCTC CAAGGGCTAC 
ATGGGCGAGT GCGGCTTCCG CGGCGGCTAC GTGGAGGTGG TGAATATGGA CGCTGCGGTG 
AAGCAGCAGA TGCAGAAGCT GCGGAGCGTG CGGCTGTGCC CACCCACCCC GGGCCAGGTC 
CTGCTCGACG TGGCTGTCAG CCCGCCGGCG CCCTCAGACC CCTCCTTCCC GCGGTTCCAG 
GCGCAGGAGA GGAGGGCGGT GCTGGCCGAG CTGGCTGCCA AGGCCAAGCT CACGGAGCAG 
GTCTTCAACG AAGCTCCCGG CATCCGCTGC AATCCGGTGC AGGGCGCCAT GTATTCGTTC 
CCGCGCGTGC AGCTGCCCCC GCGTGCGGTG CAGCGCGCTC AGGTCCGAAG AGCTGGGCCT 
GGCTCCCGAC ATGTTCTTCT GCCTGCGCCT CCTAGAGGAG ACTGGCATCT GCGTGGTGCC 
TGGGAGTGGC TTTGGACAAC GGGAAGGCAC CTACCACTTT CGGATGACTA TTCTGCCCCC 
CATGGAGAAG CTACGGCCCC TGCTGGAGAA GCTGAGCCAG TTCCATGCGA AGTTCACCCG 
CGAGTACTCC TGAGGACTCG TCAGAGGCCA GGGCCGGCCA GTAGGGACGA CCTCCGACTG 
AGGGCACTCT GCCCTGGGGC CTCTGGATTT GCTCCTGCTG GCCGTGTGGC CGAGCCCCTG 
CCCCTCCGCA GGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329842.1
CDS	980..3577
Translation	MGPIAPCRACRGGPRGLSQLPSHSLHQSGASGRGHLGHLAPGAAPRAAPRGAAPRAAPGWYVFGKRAGQQPRAAEVSRGPCEGPELPSTPTPSTPAQRLQPCSTDGGPSSRHRLLLAQAWPQRRRASLPSPNSPKMPEAWLSYPGDRPALLAGSKDGGSERAGPRPSPTGVTMLRSWALVPLAWGPSPPSSVDPSGRSPPPCPQAWSHRAAGTFCLSLPHVGLLSPVLGTLGGHRASPQAPPAFSPTLCGSQGRSVPRWAGPGSPAPSASSVLSVGPAGCSQEGAPSQAPPSQPWPIEPGPADLTPASGSCCLLSLLPAHLSRVMALRAGEHSQEAANGLKEKVLTLDSMNPYVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQIPITFPRQVLALCVHPDLLNSPDFPDDAKRRAERILQACGGHSLGAYSISAGVQMIREDVARYIERRDGGIPADPNNIFLSTGASDAIVTVLKLLVTGEGRTRTGVLIPIPQYPLYSAALAEFNAVQVDYYLDEERAWALDVAELRRALRQARDHCRPRALCVINPGNPTGQVQTRECIEDVIRFAYEEKLFLLADEVYQDNVYAESSQFHSFKKVLTEMGPPYAAQQELASFHSISKGYMGECGFRGGYVEVVNMDAAVKQQMQKLRSVRLCPPTPGQVLLDVAVSPPAPSDPSFPRFQAQERRAVLAELAAKAKLTEQVFNEAPGIRCNPVQGAMYSFPRVQLPPRAVQRAQVRRAGPGSRHVLLPAPPRGDWHLRGAWEWLWTTGRHLPLSDDYSAPHGEATAPAGEAEPVPCEVHPRVLLRTRQRPGPASRDDLRLRALCPGASGFAPAGRVAEPLPLRRP

Target ORF information:

RefSeq Version	XM_015474356.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474356.1

ORF Insert Sequence:

ATGGGGCCTA TCGCTCCCTG CAGGGCCTGC AGAGGTGGGC CCCGAGGCTT ATCACAGCTC 
CCTTCCCACT CTTTGCACCA ATCAGGAGCC TCAGGGAGGG GGCACTTAGG TCACCTTGCC 
CCTGGTGCAG CCCCAAGGGC AGCTCCACGC GGTGCAGCCC CAAGGGCAGC TCCAGGCTGG 
TACGTGTTTG GCAAAAGGGC AGGACAGCAG CCCAGAGCTG CAGAGGTGTC CCGAGGGCCC 
TGCGAAGGCC CGGAGCTGCC CAGCACCCCC ACTCCCAGCA CCCCAGCTCA GAGACTCCAG 
CCTTGCAGCA CCGATGGGGG ACCAAGCTCC AGACACAGGC TCTTGCTGGC CCAGGCCTGG 
CCCCAGCGCA GGAGGGCCTC GCTCCCCTCC CCCAACAGCC CCAAGATGCC TGAGGCCTGG 
CTCAGTTACC CGGGAGACAG GCCTGCTCTG CTGGCCGGGA GCAAAGATGG GGGGTCCGAG 
AGGGCTGGGC CTCGGCCGAG CCCTACTGGG GTAACTATGC TGAGGTCCTG GGCCTTAGTC 
CCGCTCGCTT GGGGGCCCAG CCCTCCCAGC TCTGTGGACC CCTCAGGGCG CTCACCCCCT 
CCCTGCCCGC AGGCCTGGTC CCACAGAGCT GCTGGCACCT TCTGCCTGTC CCTGCCCCAT 
GTGGGTCTCC TGTCCCCTGT CCTGGGGACG CTGGGCGGGC ACCGGGCCTC ACCCCAAGCC 
CCGCCTGCCT TCTCACCCAC TCTCTGTGGC TCCCAGGGCA GATCTGTGCC CAGGTGGGCG 
GGGCCTGGCA GTCCGGCTCC TTCAGCCTCT TCTGTCCTCT CAGTGGGGCC AGCAGGCTGC 
AGCCAAGAGG GCGCCCCTTC CCAGGCTCCC CCTTCCCAGC CCTGGCCCAT CGAGCCTGGA 
CCGGCTGACC TCACCCCTGC TTCTGGATCC TGCTGCCTCC TGAGCCTCCT TCCTGCCCAT 
CTGAGCAGAG TCATGGCCTT GAGGGCAGGT GAGCACAGCC AGGAGGCAGC AAATGGGCTG 
AAGGAGAAGG TGCTGACACT GGACTCCATG AATCCTTATG TCCGGAGGGT GGAGTATGCG 
GTGCGAGGCC CCATCGTTCA GCGGGCACTG GAGCTGGAGC AGGAGCTGCG CCAGGGCGTA 
AAGAAGCCCT TCACCGAGGT CATCCGCGCC AACATCGGGG ACGCACAGGC CATGGGGCAG 
ATCCCTATCA CCTTCCCGCG CCAGGTCCTG GCCCTCTGCG TCCATCCCGA TCTCCTGAAC 
AGCCCCGACT TCCCCGACGA CGCCAAGAGG AGGGCGGAGC GCATCTTGCA GGCGTGTGGG 
GGCCACAGCC TGGGGGCCTA CAGCATCAGC GCTGGCGTCC AGATGATCCG TGAGGATGTG 
GCGCGGTACA TCGAGCGGCG CGATGGAGGC ATTCCTGCCG ACCCCAATAA CATCTTTCTG 
TCCACGGGGG CCAGCGATGC CATTGTGACG GTGCTGAAGT TGCTGGTAAC CGGCGAGGGT 
CGCACGCGCA CGGGCGTGCT TATCCCTATC CCTCAGTATC CACTCTACTC CGCCGCGCTG 
GCCGAGTTCA ATGCGGTGCA GGTGGACTAC TACCTGGACG AGGAGCGTGC CTGGGCGCTC 
GATGTGGCCG AGCTGCGGCG CGCGCTGCGC CAGGCGCGTG ACCACTGCCG CCCCCGCGCG 
CTCTGCGTCA TCAACCCCGG GAACCCCACC GGGCAGGTGC AGACCCGCGA GTGCATCGAG 
GACGTGATCC GCTTCGCCTA TGAGGAGAAG CTTTTCCTAT TGGCCGATGA GGTGTACCAA 
GACAACGTGT ATGCCGAGAG CTCGCAGTTC CACTCGTTCA AGAAGGTGCT CACGGAGATG 
GGGCCGCCGT ACGCGGCGCA ACAGGAGCTC GCCTCCTTCC ACTCGATCTC CAAGGGCTAC 
ATGGGCGAGT GCGGCTTCCG CGGCGGCTAC GTGGAGGTGG TGAATATGGA CGCTGCGGTG 
AAGCAGCAGA TGCAGAAGCT GCGGAGCGTG CGGCTGTGCC CACCCACCCC GGGCCAGGTC 
CTGCTCGACG TGGCTGTCAG CCCGCCGGCG CCCTCAGACC CCTCCTTCCC GCGGTTCCAG 
GCGCAGGAGA GGAGGGCGGT GCTGGCCGAG CTGGCTGCCA AGGCCAAGCT CACGGAGCAG 
GTCTTCAACG AAGCTCCCGG CATCCGCTGC AATCCGGTGC AGGGCGCCAT GTATTCGTTC 
CCGCGCGTGC AGCTGCCCCC GCGTGCGGTG CAGCGCGCTC AGGTCCGAAG AGCTGGGCCT 
GGCTCCCGAC ATGTTCTTCT GCCTGCGCCT CCTAGAGGAG ACTGGCATCT GCGTGGTGCC 
TGGGAGTGGC TTTGGACAAC GGGAAGGCAC CTACCACTTT CGGATGACTA TTCTGCCCCC 
CATGGAGAAG CTACGGCCCC TGCTGGAGAA GCTGAGCCAG TTCCATGCGA AGTTCACCCG 
CGAGTACTCC TGAGGACTCG TCAGAGGCCA GGGCCGGCCA GTAGGGACGA CCTCCGACTG 
AGGGCACTCT GCCCTGGGGC CTCTGGATTT GCTCCTGCTG GCCGTGTGGC CGAGCCCCTG 
CCCCTCCGCA GGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPT ( XM_015474357.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329843.1 Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa92455
Clone ID Related Accession (Same CDS sequence)	XM_015474357.1
Accession Version	XM_015474357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	alanine aminotransferase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCTA TCGCTCCCTG CAGGGCCTGC AGAGGTGGGC CCCGAGGCTT ATCACAGCTC 
CCTTCCCACT CTTTGCACCA ATCAGGAGCC TCAGGGAGGG GGCACTTAGG TCACCTTGCC 
CCTGGTGCAG CCCCAAGGGC AGCTCCACGC GGTGCAGCCC CAAGGGCAGC TCCAGGCTGG 
TACGTGTTTG GCAAAAGGGC AGGACAGCAG CCCAGAGCTG CAGAGGTGTC CCGAGGGCCC 
TGCGAAGGCC CGGAGCTGCC CAGCACCCCC ACTCCCAGCA CCCCAGCTCA GAGACTCCAG 
CCTTGCAGCA CCGATGGGGG ACCAAGCTCC AGACACAGGC TCTTGCTGGC CCAGGCCTGG 
CCCCAGCGCA GGAGGGCCTC GCTCCCCTCC CCCAACAGCC CCAAGATGCC TGAGGCCTGG 
CTCAGTTACC CGGGAGACAG GCCTGCTCTG CTGGCCGGGA GCAAAGATGG GGGGTCCGAG 
AGGGCTGGGC CTCGGCCGAG CCCTACTGGG GTAACTATGC TGAGGTCCTG GGCCTTAGTC 
CCGCTCGCTT GGGGGCCCAG CCCTCCCAGC TCTGTGGACC CCTCAGGGCG CTCACCCCCT 
CCCTGCCCGC AGGCCTGGTC CCACAGAGCT GCTGGCACCT TCTGCCTGTC CCTGCCCCAT 
GTGGGTCTCC TGTCCCCTGT CCTGGGGACG CTGGGCGGGC ACCGGGCCTC ACCCCAAGCC 
CCGCCTGCCT TCTCACCCAC TCTCTGTGGC TCCCAGGGCA GATCTGTGCC CAGGTGGGCG 
GGGCCTGGCA GTCCGGCTCC TTCAGCCTCT TCTGTCCTCT CAGTGGGGCC AGCAGGCTGC 
AGCCAAGAGG GCGCCCCTTC CCAGGCTCCC CCTTCCCAGC CCTGGCCCAT CGAGCCTGGA 
CCGGCTGACC TCACCCCTGC TTCTGGATCC TGCTGCCTCC TGAGCCTCCT TCCTGCCCAT 
CTGAGCAGAG TCATGGCCTT GAGGGCAGGT GAGCACAGCC AGGAGGCAGC AAATGGGCTG 
AAGGAGAAGG TGCTGACACT GGACTCCATG AATCCTTATG TCCGGAGGGT GGAGTATGCG 
GTGCGAGGCC CCATCGTTCA GCGGGCACTG GAGCTGGAGC AGGAGCTGCG CCAGGGCGTA 
AAGAAGCCCT TCACCGAGGT CATCCGCGCC AACATCGGGG ACGCACAGGC CATGGGGCAG 
ATCCCTATCA CCTTCCCGCG CCAGGTCCTG GCCCTCTGCG TCCATCCCGA TCTCCTGAAC 
AGCCCCGACT TCCCCGACGA CGCCAAGAGG AGGGCGGAGC GCATCTTGCA GGCGTGTGGG 
GGCCACAGCC TGGGGGCCTA CAGCATCAGC GCTGGCGTCC AGATGATCCG TGAGGATGTG 
GCGCGGTACA TCGAGCGGCG CGATGGAGGC ATTCCTGCCG ACCCCAATAA CATCTTTCTG 
TCCACGGGGG CCAGCGATGC CATTGTGACG GTGCTGAAGT TGCTGGTAAC CGGCGAGGGT 
CGCACGCGCA CGGGCGTGCT TATCCCTATC CCTCAGTATC CACTCTACTC CGCCGCGCTG 
GCCGAGTTCA ATGCGGTGCA GGTGGACTAC TACCTGGACG AGGAGCGTGC CTGGGCGCTC 
GATGTGGCCG AGCTGCGGCG CGCGCTGCGC CAGGCGCGTG ACCACTGCCG CCCCCGCGCG 
CTCTGCGTCA TCAACCCCGG GAACCCCACC GGGCAGGTGC AGACCCGCGA GTGCATCGAG 
GACGTGATCC GCTTCGCCTA TGAGGAGAAG CTTTTCCTAT TGGCCGATGA GGTGTACCAA 
GACAACGTGT ATGCCGAGAG CTCGCAGTTC CACTCGTTCA AGAAGGTGCT CACGGAGATG 
GGGCCGCCGT ACGCGGCGCA ACAGGAGCTC GCCTCCTTCC ACTCGATCTC CAAGGGCTAC 
ATGGGCGAGT GCGGCTTCCG CGGCGGCTAC GTGGAGGTGG TGAATATGGA CGCTGCGGTG 
AAGCAGCAGA TGCAGAAGCT GCGGAGCGTG CGGCTGTGCC CACCCACCCC GGGCCAGGTC 
CTGCTCGACG TGGCTGTCAG CCCGCCGGCG CCCTCAGACC CCTCCTTCCC GCGGTTCCAG 
GCGGAGAGGA GGGCGGTGCT GGCCGAGCTG GCTGCCAAGG CCAAGCTCAC GGAGCAGGTC 
TTCAACGAAG CTCCCGGCAT CCGCTGCAAT CCGGTGCAGG GCGCCATGTA TTCGTTCCCG 
CGCGTGCAGC TGCCCCCGCG TGCGGTGCAG CGCGCTCAGG TCCGAAGAGC TGGGCCTGGC 
TCCCGACATG TTCTTCTGCC TGCGCCTCCT AGAGGAGACT GGCATCTGCG TGGTGCCTGG 
GAGTGGCTTT GGACAACGGG AAGGCACCTA CCACTTTCGG ATGACTATTC TGCCCCCCAT 
GGAGAAGCTA CGGCCCCTGC TGGAGAAGCT GAGCCAGTTC CATGCGAAGT TCACCCGCGA 
GTACTCCTGA GGACTCGTCA GAGGCCAGGG CCGGCCAGTA GGGACGACCT CCGACTGAGG 
GCACTCTGCC CTGGGGCCTC TGGATTTGCT CCTGCTGGCC GTGTGGCCGA GCCCCTGCCC 
CTCCGCAGGC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329843.1
CDS	980..3574
Translation	MGPIAPCRACRGGPRGLSQLPSHSLHQSGASGRGHLGHLAPGAAPRAAPRGAAPRAAPGWYVFGKRAGQQPRAAEVSRGPCEGPELPSTPTPSTPAQRLQPCSTDGGPSSRHRLLLAQAWPQRRRASLPSPNSPKMPEAWLSYPGDRPALLAGSKDGGSERAGPRPSPTGVTMLRSWALVPLAWGPSPPSSVDPSGRSPPPCPQAWSHRAAGTFCLSLPHVGLLSPVLGTLGGHRASPQAPPAFSPTLCGSQGRSVPRWAGPGSPAPSASSVLSVGPAGCSQEGAPSQAPPSQPWPIEPGPADLTPASGSCCLLSLLPAHLSRVMALRAGEHSQEAANGLKEKVLTLDSMNPYVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQIPITFPRQVLALCVHPDLLNSPDFPDDAKRRAERILQACGGHSLGAYSISAGVQMIREDVARYIERRDGGIPADPNNIFLSTGASDAIVTVLKLLVTGEGRTRTGVLIPIPQYPLYSAALAEFNAVQVDYYLDEERAWALDVAELRRALRQARDHCRPRALCVINPGNPTGQVQTRECIEDVIRFAYEEKLFLLADEVYQDNVYAESSQFHSFKKVLTEMGPPYAAQQELASFHSISKGYMGECGFRGGYVEVVNMDAAVKQQMQKLRSVRLCPPTPGQVLLDVAVSPPAPSDPSFPRFQAERRAVLAELAAKAKLTEQVFNEAPGIRCNPVQGAMYSFPRVQLPPRAVQRAQVRRAGPGSRHVLLPAPPRGDWHLRGAWEWLWTTGRHLPLSDDYSAPHGEATAPAGEAEPVPCEVHPRVLLRTRQRPGPASRDDLRLRALCPGASGFAPAGRVAEPLPLRRP

Target ORF information:

RefSeq Version	XM_015474357.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474357.1

ORF Insert Sequence:

ATGGGGCCTA TCGCTCCCTG CAGGGCCTGC AGAGGTGGGC CCCGAGGCTT ATCACAGCTC 
CCTTCCCACT CTTTGCACCA ATCAGGAGCC TCAGGGAGGG GGCACTTAGG TCACCTTGCC 
CCTGGTGCAG CCCCAAGGGC AGCTCCACGC GGTGCAGCCC CAAGGGCAGC TCCAGGCTGG 
TACGTGTTTG GCAAAAGGGC AGGACAGCAG CCCAGAGCTG CAGAGGTGTC CCGAGGGCCC 
TGCGAAGGCC CGGAGCTGCC CAGCACCCCC ACTCCCAGCA CCCCAGCTCA GAGACTCCAG 
CCTTGCAGCA CCGATGGGGG ACCAAGCTCC AGACACAGGC TCTTGCTGGC CCAGGCCTGG 
CCCCAGCGCA GGAGGGCCTC GCTCCCCTCC CCCAACAGCC CCAAGATGCC TGAGGCCTGG 
CTCAGTTACC CGGGAGACAG GCCTGCTCTG CTGGCCGGGA GCAAAGATGG GGGGTCCGAG 
AGGGCTGGGC CTCGGCCGAG CCCTACTGGG GTAACTATGC TGAGGTCCTG GGCCTTAGTC 
CCGCTCGCTT GGGGGCCCAG CCCTCCCAGC TCTGTGGACC CCTCAGGGCG CTCACCCCCT 
CCCTGCCCGC AGGCCTGGTC CCACAGAGCT GCTGGCACCT TCTGCCTGTC CCTGCCCCAT 
GTGGGTCTCC TGTCCCCTGT CCTGGGGACG CTGGGCGGGC ACCGGGCCTC ACCCCAAGCC 
CCGCCTGCCT TCTCACCCAC TCTCTGTGGC TCCCAGGGCA GATCTGTGCC CAGGTGGGCG 
GGGCCTGGCA GTCCGGCTCC TTCAGCCTCT TCTGTCCTCT CAGTGGGGCC AGCAGGCTGC 
AGCCAAGAGG GCGCCCCTTC CCAGGCTCCC CCTTCCCAGC CCTGGCCCAT CGAGCCTGGA 
CCGGCTGACC TCACCCCTGC TTCTGGATCC TGCTGCCTCC TGAGCCTCCT TCCTGCCCAT 
CTGAGCAGAG TCATGGCCTT GAGGGCAGGT GAGCACAGCC AGGAGGCAGC AAATGGGCTG 
AAGGAGAAGG TGCTGACACT GGACTCCATG AATCCTTATG TCCGGAGGGT GGAGTATGCG 
GTGCGAGGCC CCATCGTTCA GCGGGCACTG GAGCTGGAGC AGGAGCTGCG CCAGGGCGTA 
AAGAAGCCCT TCACCGAGGT CATCCGCGCC AACATCGGGG ACGCACAGGC CATGGGGCAG 
ATCCCTATCA CCTTCCCGCG CCAGGTCCTG GCCCTCTGCG TCCATCCCGA TCTCCTGAAC 
AGCCCCGACT TCCCCGACGA CGCCAAGAGG AGGGCGGAGC GCATCTTGCA GGCGTGTGGG 
GGCCACAGCC TGGGGGCCTA CAGCATCAGC GCTGGCGTCC AGATGATCCG TGAGGATGTG 
GCGCGGTACA TCGAGCGGCG CGATGGAGGC ATTCCTGCCG ACCCCAATAA CATCTTTCTG 
TCCACGGGGG CCAGCGATGC CATTGTGACG GTGCTGAAGT TGCTGGTAAC CGGCGAGGGT 
CGCACGCGCA CGGGCGTGCT TATCCCTATC CCTCAGTATC CACTCTACTC CGCCGCGCTG 
GCCGAGTTCA ATGCGGTGCA GGTGGACTAC TACCTGGACG AGGAGCGTGC CTGGGCGCTC 
GATGTGGCCG AGCTGCGGCG CGCGCTGCGC CAGGCGCGTG ACCACTGCCG CCCCCGCGCG 
CTCTGCGTCA TCAACCCCGG GAACCCCACC GGGCAGGTGC AGACCCGCGA GTGCATCGAG 
GACGTGATCC GCTTCGCCTA TGAGGAGAAG CTTTTCCTAT TGGCCGATGA GGTGTACCAA 
GACAACGTGT ATGCCGAGAG CTCGCAGTTC CACTCGTTCA AGAAGGTGCT CACGGAGATG 
GGGCCGCCGT ACGCGGCGCA ACAGGAGCTC GCCTCCTTCC ACTCGATCTC CAAGGGCTAC 
ATGGGCGAGT GCGGCTTCCG CGGCGGCTAC GTGGAGGTGG TGAATATGGA CGCTGCGGTG 
AAGCAGCAGA TGCAGAAGCT GCGGAGCGTG CGGCTGTGCC CACCCACCCC GGGCCAGGTC 
CTGCTCGACG TGGCTGTCAG CCCGCCGGCG CCCTCAGACC CCTCCTTCCC GCGGTTCCAG 
GCGGAGAGGA GGGCGGTGCT GGCCGAGCTG GCTGCCAAGG CCAAGCTCAC GGAGCAGGTC 
TTCAACGAAG CTCCCGGCAT CCGCTGCAAT CCGGTGCAGG GCGCCATGTA TTCGTTCCCG 
CGCGTGCAGC TGCCCCCGCG TGCGGTGCAG CGCGCTCAGG TCCGAAGAGC TGGGCCTGGC 
TCCCGACATG TTCTTCTGCC TGCGCCTCCT AGAGGAGACT GGCATCTGCG TGGTGCCTGG 
GAGTGGCTTT GGACAACGGG AAGGCACCTA CCACTTTCGG ATGACTATTC TGCCCCCCAT 
GGAGAAGCTA CGGCCCCTGC TGGAGAAGCT GAGCCAGTTC CATGCGAAGT TCACCCGCGA 
GTACTCCTGA GGACTCGTCA GAGGCCAGGG CCGGCCAGTA GGGACGACCT CCGACTGAGG 
GCACTCTGCC CTGGGGCCTC TGGATTTGCT CCTGCTGGCC GTGTGGCCGA GCCCCTGCCC 
CTCCGCAGGC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPT ( XM_015474358.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329844.1 Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa92456
Clone ID Related Accession (Same CDS sequence)	XM_015474358.1
Accession Version	XM_015474358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	alanine aminotransferase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCTA TCGCTCCCTG CAGGGCCTGC AGAGGTGGGC CCCGAGGCTT ATCACAGCTC 
CCTTCCCACT CTTTGCACCA ATCAGGAGCC TCAGGGAGGG GGCACTTAGG TCACCTTGCC 
CCTGGTGCAG CCCCAAGGGC AGCTCCACGC GGTGCAGCCC CAAGGGCAGC TCCAGGCTGG 
TACGTGTTTG GCAAAAGGGC AGGACAGCAG CCCAGAGCTG CAGAGGTGTC CCGAGGGCCC 
TGCGAAGGCC CGGAGCTGCC CAGCACCCCC ACTCCCAGCA CCCCAGCTCA GAGACTCCAG 
CCTTGCAGCA CCGATGGGGG ACCAAGCTCC AGACACAGGC TCTTGCTGGC CCAGGCCTGG 
CCCCAGCGCA GGAGGGCCTC GCTCCCCTCC CCCAACAGCC CCAAGATGCC TGAGGCCTGG 
CTCAGTTACC CGGGAGACAG GCCTGCTCTG CTGGCCGGGA GCAAAGATGG GGGGTCCGAG 
AGGGCTGGGC CTCGGCCGAG CCCTACTGGG GTAACTATGC TGAGGTCCTG GGCCTTAGTC 
CCGCTCGCTT GGGGGCCCAG CCCTCCCAGC TCTGTGGACC CCTCAGGGCG CTCACCCCCT 
CCCTGCCCGC AGGCCTGGTC CCACAGAGCT GCTGGCACCT TCTGCCTGTC CCTGCCCCAT 
GTGGGTCTCC TGTCCCCTGT CCTGGGGACG CTGGGCGGGC ACCGGGCCTC ACCCCAAGCC 
CCGCCTGCCT TCTCACCCAC TCTCTGTGGC TCCCAGGGCA GATCTGTGCC CAGGTGGGCG 
GGGCCTGGCA GTCCGGCTCC TTCAGCCTCT TCTGTCCTCT CAGTGGGGCC AGCAGGCTGC 
AGCCAAGAGG GCGCCCCTTC CCAGGCTCCC CCTTCCCAGC CCTGGCCCAT CGAGCCTGGA 
CCGGCTGACC TCACCCCTGC TTCTGGATCC TGCTGCCTCC TGAGCCTCCT TCCTGCCCAT 
CTGAGCAGAG TCATGGCCTT GAGGGCAGGT GAGCACAGCC AGGAGGCAGC AAATGGGCTG 
AAGGAGAAGG TGCTGACACT GGACTCCATG AATCCTTATG TCCGGAGGGT GGAGTATGCG 
GTGCGAGGCC CCATCGTTCA GCGGGCACTG GAGCTGGAGC AGGAGCTGCG CCAGGGCGTA 
AAGAAGCCCT TCACCGAGGT CATCCGCGCC AACATCGGGG ACGCACAGGC CATGGGGCAG 
ATCCCTATCA CCTTCCCGCG CCAGGTCCTG GCCCTCTGCG TCCATCCCGA TCTCCTGAAC 
AGCCCCGACT TCCCCGACGA CGCCAAGAGG AGGGCGGAGC GCATCTTGCA GGCGTGTGGG 
GGCCACAGCC TGGGGGCCTA CAGCATCAGC GCTGGCGTCC AGATGATCCG TGAGGATGTG 
GCGCGGTACA TCGAGCGGCG CGATGGAGGC ATTCCTGCCG ACCCCAATAA CATCTTTCTG 
TCCACGGGGG CCAGCGATGC CATTGTGACG GTGCTGAAGT TGCTGGTAAC CGGCGAGGGT 
CGCACGCGCA CGGGCGTGCT TATCCCTATC CCTCAGTATC CACTCTACTC CGCCGCGCTG 
GCCGAGTTCA ATGCGGTGCA GGTGGACTAC TACCTGGACG AGGAGCGTGC CTGGGCGCTC 
GATGTGGCCG AGCTGCGGCG CGCGCTGCGC CAGGCGCGTG ACCACTGCCG CCCCCGCGCG 
CTCTGCGTCA TCAACCCCGG GAACCCCACC GGGCAGGTGC AGACCCGCGA GTGCATCGAG 
GACGTGATCC GCTTCGCCTA TGAGGAGAAG CTTTTCCTAT TGGCCGATGA GGTGTACCAA 
GACAACGTGT ATGCCGAGAG CTCGCAGTTC CACTCGTTCA AGAAGGTGCT CACGGAGATG 
GGGCCGCCGT ACGCGGCGCA ACAGGAGCTC GCCTCCTTCC ACTCGATCTC CAAGGGCTAC 
ATGGGCGAGT GCGGCTTCCG CGGCGGCTAC GTGGAGGTGG TGAATATGGA CGCTGCGGTG 
AAGCAGCAGA TGCAGAAGCT GCGGAGCGTG CGGCTGTGCC CACCCACCCC GGGCCAGGTC 
CTGCTCGACG TGGCTGTCAG CCCGCCGGCG CCCTCAGACC CCTCCTTCCC GCGGTTCCAG 
GCGCAGGAGA GGAGGGCGGT GCTGGCCGAG CTGGCTGCCA AGGCCAAGCT CACGGAGCAG 
GTCTTCAACG AAGCTCCCGG CATCCGCTGC AATCCGGTGC AGGGCGCCAT GTATTCGTTC 
CCGCGCGTGC AGCTGCCCCC GCGTGCGGTG CAGCGCGCTC AGGAGCTGGG CCTGGCTCCC 
GACATGTTCT TCTGCCTGCG CCTCCTAGAG GAGACTGGCA TCTGCGTGGT GCCTGGGAGT 
GGCTTTGGAC AACGGGAAGG CACCTACCAC TTTCGGATGA CTATTCTGCC CCCCATGGAG 
AAGCTACGGC CCCTGCTGGA GAAGCTGAGC CAGTTCCATG CGAAGTTCAC CCGCGAGTAC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329844.1
CDS	980..3445
Translation	MGPIAPCRACRGGPRGLSQLPSHSLHQSGASGRGHLGHLAPGAAPRAAPRGAAPRAAPGWYVFGKRAGQQPRAAEVSRGPCEGPELPSTPTPSTPAQRLQPCSTDGGPSSRHRLLLAQAWPQRRRASLPSPNSPKMPEAWLSYPGDRPALLAGSKDGGSERAGPRPSPTGVTMLRSWALVPLAWGPSPPSSVDPSGRSPPPCPQAWSHRAAGTFCLSLPHVGLLSPVLGTLGGHRASPQAPPAFSPTLCGSQGRSVPRWAGPGSPAPSASSVLSVGPAGCSQEGAPSQAPPSQPWPIEPGPADLTPASGSCCLLSLLPAHLSRVMALRAGEHSQEAANGLKEKVLTLDSMNPYVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQIPITFPRQVLALCVHPDLLNSPDFPDDAKRRAERILQACGGHSLGAYSISAGVQMIREDVARYIERRDGGIPADPNNIFLSTGASDAIVTVLKLLVTGEGRTRTGVLIPIPQYPLYSAALAEFNAVQVDYYLDEERAWALDVAELRRALRQARDHCRPRALCVINPGNPTGQVQTRECIEDVIRFAYEEKLFLLADEVYQDNVYAESSQFHSFKKVLTEMGPPYAAQQELASFHSISKGYMGECGFRGGYVEVVNMDAAVKQQMQKLRSVRLCPPTPGQVLLDVAVSPPAPSDPSFPRFQAQERRAVLAELAAKAKLTEQVFNEAPGIRCNPVQGAMYSFPRVQLPPRAVQRAQELGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPMEKLRPLLEKLSQFHAKFTREYS

Target ORF information:

RefSeq Version	XM_015474358.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474358.1

ORF Insert Sequence:

ATGGGGCCTA TCGCTCCCTG CAGGGCCTGC AGAGGTGGGC CCCGAGGCTT ATCACAGCTC 
CCTTCCCACT CTTTGCACCA ATCAGGAGCC TCAGGGAGGG GGCACTTAGG TCACCTTGCC 
CCTGGTGCAG CCCCAAGGGC AGCTCCACGC GGTGCAGCCC CAAGGGCAGC TCCAGGCTGG 
TACGTGTTTG GCAAAAGGGC AGGACAGCAG CCCAGAGCTG CAGAGGTGTC CCGAGGGCCC 
TGCGAAGGCC CGGAGCTGCC CAGCACCCCC ACTCCCAGCA CCCCAGCTCA GAGACTCCAG 
CCTTGCAGCA CCGATGGGGG ACCAAGCTCC AGACACAGGC TCTTGCTGGC CCAGGCCTGG 
CCCCAGCGCA GGAGGGCCTC GCTCCCCTCC CCCAACAGCC CCAAGATGCC TGAGGCCTGG 
CTCAGTTACC CGGGAGACAG GCCTGCTCTG CTGGCCGGGA GCAAAGATGG GGGGTCCGAG 
AGGGCTGGGC CTCGGCCGAG CCCTACTGGG GTAACTATGC TGAGGTCCTG GGCCTTAGTC 
CCGCTCGCTT GGGGGCCCAG CCCTCCCAGC TCTGTGGACC CCTCAGGGCG CTCACCCCCT 
CCCTGCCCGC AGGCCTGGTC CCACAGAGCT GCTGGCACCT TCTGCCTGTC CCTGCCCCAT 
GTGGGTCTCC TGTCCCCTGT CCTGGGGACG CTGGGCGGGC ACCGGGCCTC ACCCCAAGCC 
CCGCCTGCCT TCTCACCCAC TCTCTGTGGC TCCCAGGGCA GATCTGTGCC CAGGTGGGCG 
GGGCCTGGCA GTCCGGCTCC TTCAGCCTCT TCTGTCCTCT CAGTGGGGCC AGCAGGCTGC 
AGCCAAGAGG GCGCCCCTTC CCAGGCTCCC CCTTCCCAGC CCTGGCCCAT CGAGCCTGGA 
CCGGCTGACC TCACCCCTGC TTCTGGATCC TGCTGCCTCC TGAGCCTCCT TCCTGCCCAT 
CTGAGCAGAG TCATGGCCTT GAGGGCAGGT GAGCACAGCC AGGAGGCAGC AAATGGGCTG 
AAGGAGAAGG TGCTGACACT GGACTCCATG AATCCTTATG TCCGGAGGGT GGAGTATGCG 
GTGCGAGGCC CCATCGTTCA GCGGGCACTG GAGCTGGAGC AGGAGCTGCG CCAGGGCGTA 
AAGAAGCCCT TCACCGAGGT CATCCGCGCC AACATCGGGG ACGCACAGGC CATGGGGCAG 
ATCCCTATCA CCTTCCCGCG CCAGGTCCTG GCCCTCTGCG TCCATCCCGA TCTCCTGAAC 
AGCCCCGACT TCCCCGACGA CGCCAAGAGG AGGGCGGAGC GCATCTTGCA GGCGTGTGGG 
GGCCACAGCC TGGGGGCCTA CAGCATCAGC GCTGGCGTCC AGATGATCCG TGAGGATGTG 
GCGCGGTACA TCGAGCGGCG CGATGGAGGC ATTCCTGCCG ACCCCAATAA CATCTTTCTG 
TCCACGGGGG CCAGCGATGC CATTGTGACG GTGCTGAAGT TGCTGGTAAC CGGCGAGGGT 
CGCACGCGCA CGGGCGTGCT TATCCCTATC CCTCAGTATC CACTCTACTC CGCCGCGCTG 
GCCGAGTTCA ATGCGGTGCA GGTGGACTAC TACCTGGACG AGGAGCGTGC CTGGGCGCTC 
GATGTGGCCG AGCTGCGGCG CGCGCTGCGC CAGGCGCGTG ACCACTGCCG CCCCCGCGCG 
CTCTGCGTCA TCAACCCCGG GAACCCCACC GGGCAGGTGC AGACCCGCGA GTGCATCGAG 
GACGTGATCC GCTTCGCCTA TGAGGAGAAG CTTTTCCTAT TGGCCGATGA GGTGTACCAA 
GACAACGTGT ATGCCGAGAG CTCGCAGTTC CACTCGTTCA AGAAGGTGCT CACGGAGATG 
GGGCCGCCGT ACGCGGCGCA ACAGGAGCTC GCCTCCTTCC ACTCGATCTC CAAGGGCTAC 
ATGGGCGAGT GCGGCTTCCG CGGCGGCTAC GTGGAGGTGG TGAATATGGA CGCTGCGGTG 
AAGCAGCAGA TGCAGAAGCT GCGGAGCGTG CGGCTGTGCC CACCCACCCC GGGCCAGGTC 
CTGCTCGACG TGGCTGTCAG CCCGCCGGCG CCCTCAGACC CCTCCTTCCC GCGGTTCCAG 
GCGCAGGAGA GGAGGGCGGT GCTGGCCGAG CTGGCTGCCA AGGCCAAGCT CACGGAGCAG 
GTCTTCAACG AAGCTCCCGG CATCCGCTGC AATCCGGTGC AGGGCGCCAT GTATTCGTTC 
CCGCGCGTGC AGCTGCCCCC GCGTGCGGTG CAGCGCGCTC AGGAGCTGGG CCTGGCTCCC 
GACATGTTCT TCTGCCTGCG CCTCCTAGAG GAGACTGGCA TCTGCGTGGT GCCTGGGAGT 
GGCTTTGGAC AACGGGAAGG CACCTACCAC TTTCGGATGA CTATTCTGCC CCCCATGGAG 
AAGCTACGGC CCCTGCTGGA GAAGCTGAGC CAGTTCCATG CGAAGTTCAC CCGCGAGTAC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPT ( XM_015474359.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329845.1 Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa92457
Clone ID Related Accession (Same CDS sequence)	XM_015474359.1
Accession Version	XM_015474359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	alanine aminotransferase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCTA TCGCTCCCTG CAGGGCCTGC AGAGGTGGGC CCCGAGGCTT ATCACAGCTC 
CCTTCCCACT CTTTGCACCA ATCAGGAGCC TCAGGGAGGG GGCACTTAGG TCACCTTGCC 
CCTGGTGCAG CCCCAAGGGC AGCTCCACGC GGTGCAGCCC CAAGGGCAGC TCCAGGCTGG 
TACGTGTTTG GCAAAAGGGC AGGACAGCAG CCCAGAGCTG CAGAGGTGTC CCGAGGGCCC 
TGCGAAGGCC CGGAGCTGCC CAGCACCCCC ACTCCCAGCA CCCCAGCTCA GAGACTCCAG 
CCTTGCAGCA CCGATGGGGG ACCAAGCTCC AGACACAGGC TCTTGCTGGC CCAGGCCTGG 
CCCCAGCGCA GGAGGGCCTC GCTCCCCTCC CCCAACAGCC CCAAGATGCC TGAGGCCTGG 
CTCAGTTACC CGGGAGACAG GCCTGCTCTG CTGGCCGGGA GCAAAGATGG GGGGTCCGAG 
AGGGCTGGGC CTCGGCCGAG CCCTACTGGG GTAACTATGC TGAGGTCCTG GGCCTTAGTC 
CCGCTCGCTT GGGGGCCCAG CCCTCCCAGC TCTGTGGACC CCTCAGGGCG CTCACCCCCT 
CCCTGCCCGC AGGCCTGGTC CCACAGAGCT GCTGGCACCT TCTGCCTGTC CCTGCCCCAT 
GTGGGTCTCC TGTCCCCTGT CCTGGGGACG CTGGGCGGGC ACCGGGCCTC ACCCCAAGCC 
CCGCCTGCCT TCTCACCCAC TCTCTGTGGC TCCCAGGGCA GATCTGTGCC CAGGTGGGCG 
GGGCCTGGCA GTCCGGCTCC TTCAGCCTCT TCTGTCCTCT CAGTGGGGCC AGCAGGCTGC 
AGCCAAGAGG GCGCCCCTTC CCAGGCTCCC CCTTCCCAGC CCTGGCCCAT CGAGCCTGGA 
CCGGCTGACC TCACCCCTGC TTCTGGATCC TGCTGCCTCC TGAGCCTCCT TCCTGCCCAT 
CTGAGCAGAG TCATGGCCTT GAGGGCAGGT GAGCACAGCC AGGAGGCAGC AAATGGGCTG 
AAGGAGAAGG TGCTGACACT GGACTCCATG AATCCTTATG TCCGGAGGGT GGAGTATGCG 
GTGCGAGGCC CCATCGTTCA GCGGGCACTG GAGCTGGAGC AGGAGCTGCG CCAGGGCGTA 
AAGAAGCCCT TCACCGAGGT CATCCGCGCC AACATCGGGG ACGCACAGGC CATGGGGCAG 
ATCCCTATCA CCTTCCCGCG CCAGGTCCTG GCCCTCTGCG TCCATCCCGA TCTCCTGAAC 
AGCCCCGACT TCCCCGACGA CGCCAAGAGG AGGGCGGAGC GCATCTTGCA GGCGTGTGGG 
GGCCACAGCC TGGGGGCCTA CAGCATCAGC GCTGGCGTCC AGATGATCCG TGAGGATGTG 
GCGCGGTACA TCGAGCGGCG CGATGGAGGC ATTCCTGCCG ACCCCAATAA CATCTTTCTG 
TCCACGGGGG CCAGCGATGC CATTGTGAGC AGAGCGCCCC TTCGGCTGAC CCCGGACCTG 
TCGCACCCCT GCGCCCCAGG GCAGGTGCAG ACCCGCGAGT GCATCGAGGA CGTGATCCGC 
TTCGCCTATG AGGAGAAGCT TTTCCTATTG GCCGATGAGG TGTACCAAGA CAACGTGTAT 
GCCGAGAGCT CGCAGTTCCA CTCGTTCAAG AAGGTGCTCA CGGAGATGGG GCCGCCGTAC 
GCGGCGCAAC AGGAGCTCGC CTCCTTCCAC TCGATCTCCA AGGGCTACAT GGGCGAGTGC 
GGCTTCCGCG GCGGCTACGT GGAGGTGGTG AATATGGACG CTGCGGTGAA GCAGCAGATG 
CAGAAGCTGC GGAGCGTGCG GCTGTGCCCA CCCACCCCGG GCCAGGTCCT GCTCGACGTG 
GCTGTCAGCC CGCCGGCGCC CTCAGACCCC TCCTTCCCGC GGTTCCAGGC GCAGGAGAGG 
AGGGCGGTGC TGGCCGAGCT GGCTGCCAAG GCCAAGCTCA CGGAGCAGGT CTTCAACGAA 
GCTCCCGGCA TCCGCTGCAA TCCGGTGCAG GGCGCCATGT ATTCGTTCCC GCGCGTGCAG 
CTGCCCCCGC GTGCGGTGCA GCGCGCTCAG GTCCGAAGAG CTGGGCCTGG CTCCCGACAT 
GTTCTTCTGC CTGCGCCTCC TAGAGGAGAC TGGCATCTGC GTGGTGCCTG GGAGTGGCTT 
TGGACAACGG GAAGGCACCT ACCACTTTCG GATGACTATT CTGCCCCCCA TGGAGAAGCT 
ACGGCCCCTG CTGGAGAAGC TGAGCCAGTT CCATGCGAAG TTCACCCGCG AGTACTCCTG 
AGGACTCGTC AGAGGCCAGG GCCGGCCAGT AGGGACGACC TCCGACTGAG GGCACTCTGC 
CCTGGGGCCT CTGGATTTGC TCCTGCTGGC CGTGTGGCCG AGCCCCTGCC CCTCCGCAGG 
CCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329845.1
CDS	980..3385
Translation	MGPIAPCRACRGGPRGLSQLPSHSLHQSGASGRGHLGHLAPGAAPRAAPRGAAPRAAPGWYVFGKRAGQQPRAAEVSRGPCEGPELPSTPTPSTPAQRLQPCSTDGGPSSRHRLLLAQAWPQRRRASLPSPNSPKMPEAWLSYPGDRPALLAGSKDGGSERAGPRPSPTGVTMLRSWALVPLAWGPSPPSSVDPSGRSPPPCPQAWSHRAAGTFCLSLPHVGLLSPVLGTLGGHRASPQAPPAFSPTLCGSQGRSVPRWAGPGSPAPSASSVLSVGPAGCSQEGAPSQAPPSQPWPIEPGPADLTPASGSCCLLSLLPAHLSRVMALRAGEHSQEAANGLKEKVLTLDSMNPYVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQIPITFPRQVLALCVHPDLLNSPDFPDDAKRRAERILQACGGHSLGAYSISAGVQMIREDVARYIERRDGGIPADPNNIFLSTGASDAIVSRAPLRLTPDLSHPCAPGQVQTRECIEDVIRFAYEEKLFLLADEVYQDNVYAESSQFHSFKKVLTEMGPPYAAQQELASFHSISKGYMGECGFRGGYVEVVNMDAAVKQQMQKLRSVRLCPPTPGQVLLDVAVSPPAPSDPSFPRFQAQERRAVLAELAAKAKLTEQVFNEAPGIRCNPVQGAMYSFPRVQLPPRAVQRAQVRRAGPGSRHVLLPAPPRGDWHLRGAWEWLWTTGRHLPLSDDYSAPHGEATAPAGEAEPVPCEVHPRVLLRTRQRPGPASRDDLRLRALCPGASGFAPAGRVAEPLPLRRP

Target ORF information:

RefSeq Version	XM_015474359.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474359.1

ORF Insert Sequence:

ATGGGGCCTA TCGCTCCCTG CAGGGCCTGC AGAGGTGGGC CCCGAGGCTT ATCACAGCTC 
CCTTCCCACT CTTTGCACCA ATCAGGAGCC TCAGGGAGGG GGCACTTAGG TCACCTTGCC 
CCTGGTGCAG CCCCAAGGGC AGCTCCACGC GGTGCAGCCC CAAGGGCAGC TCCAGGCTGG 
TACGTGTTTG GCAAAAGGGC AGGACAGCAG CCCAGAGCTG CAGAGGTGTC CCGAGGGCCC 
TGCGAAGGCC CGGAGCTGCC CAGCACCCCC ACTCCCAGCA CCCCAGCTCA GAGACTCCAG 
CCTTGCAGCA CCGATGGGGG ACCAAGCTCC AGACACAGGC TCTTGCTGGC CCAGGCCTGG 
CCCCAGCGCA GGAGGGCCTC GCTCCCCTCC CCCAACAGCC CCAAGATGCC TGAGGCCTGG 
CTCAGTTACC CGGGAGACAG GCCTGCTCTG CTGGCCGGGA GCAAAGATGG GGGGTCCGAG 
AGGGCTGGGC CTCGGCCGAG CCCTACTGGG GTAACTATGC TGAGGTCCTG GGCCTTAGTC 
CCGCTCGCTT GGGGGCCCAG CCCTCCCAGC TCTGTGGACC CCTCAGGGCG CTCACCCCCT 
CCCTGCCCGC AGGCCTGGTC CCACAGAGCT GCTGGCACCT TCTGCCTGTC CCTGCCCCAT 
GTGGGTCTCC TGTCCCCTGT CCTGGGGACG CTGGGCGGGC ACCGGGCCTC ACCCCAAGCC 
CCGCCTGCCT TCTCACCCAC TCTCTGTGGC TCCCAGGGCA GATCTGTGCC CAGGTGGGCG 
GGGCCTGGCA GTCCGGCTCC TTCAGCCTCT TCTGTCCTCT CAGTGGGGCC AGCAGGCTGC 
AGCCAAGAGG GCGCCCCTTC CCAGGCTCCC CCTTCCCAGC CCTGGCCCAT CGAGCCTGGA 
CCGGCTGACC TCACCCCTGC TTCTGGATCC TGCTGCCTCC TGAGCCTCCT TCCTGCCCAT 
CTGAGCAGAG TCATGGCCTT GAGGGCAGGT GAGCACAGCC AGGAGGCAGC AAATGGGCTG 
AAGGAGAAGG TGCTGACACT GGACTCCATG AATCCTTATG TCCGGAGGGT GGAGTATGCG 
GTGCGAGGCC CCATCGTTCA GCGGGCACTG GAGCTGGAGC AGGAGCTGCG CCAGGGCGTA 
AAGAAGCCCT TCACCGAGGT CATCCGCGCC AACATCGGGG ACGCACAGGC CATGGGGCAG 
ATCCCTATCA CCTTCCCGCG CCAGGTCCTG GCCCTCTGCG TCCATCCCGA TCTCCTGAAC 
AGCCCCGACT TCCCCGACGA CGCCAAGAGG AGGGCGGAGC GCATCTTGCA GGCGTGTGGG 
GGCCACAGCC TGGGGGCCTA CAGCATCAGC GCTGGCGTCC AGATGATCCG TGAGGATGTG 
GCGCGGTACA TCGAGCGGCG CGATGGAGGC ATTCCTGCCG ACCCCAATAA CATCTTTCTG 
TCCACGGGGG CCAGCGATGC CATTGTGAGC AGAGCGCCCC TTCGGCTGAC CCCGGACCTG 
TCGCACCCCT GCGCCCCAGG GCAGGTGCAG ACCCGCGAGT GCATCGAGGA CGTGATCCGC 
TTCGCCTATG AGGAGAAGCT TTTCCTATTG GCCGATGAGG TGTACCAAGA CAACGTGTAT 
GCCGAGAGCT CGCAGTTCCA CTCGTTCAAG AAGGTGCTCA CGGAGATGGG GCCGCCGTAC 
GCGGCGCAAC AGGAGCTCGC CTCCTTCCAC TCGATCTCCA AGGGCTACAT GGGCGAGTGC 
GGCTTCCGCG GCGGCTACGT GGAGGTGGTG AATATGGACG CTGCGGTGAA GCAGCAGATG 
CAGAAGCTGC GGAGCGTGCG GCTGTGCCCA CCCACCCCGG GCCAGGTCCT GCTCGACGTG 
GCTGTCAGCC CGCCGGCGCC CTCAGACCCC TCCTTCCCGC GGTTCCAGGC GCAGGAGAGG 
AGGGCGGTGC TGGCCGAGCT GGCTGCCAAG GCCAAGCTCA CGGAGCAGGT CTTCAACGAA 
GCTCCCGGCA TCCGCTGCAA TCCGGTGCAG GGCGCCATGT ATTCGTTCCC GCGCGTGCAG 
CTGCCCCCGC GTGCGGTGCA GCGCGCTCAG GTCCGAAGAG CTGGGCCTGG CTCCCGACAT 
GTTCTTCTGC CTGCGCCTCC TAGAGGAGAC TGGCATCTGC GTGGTGCCTG GGAGTGGCTT 
TGGACAACGG GAAGGCACCT ACCACTTTCG GATGACTATT CTGCCCCCCA TGGAGAAGCT 
ACGGCCCCTG CTGGAGAAGC TGAGCCAGTT CCATGCGAAG TTCACCCGCG AGTACTCCTG 
AGGACTCGTC AGAGGCCAGG GCCGGCCAGT AGGGACGACC TCCGACTGAG GGCACTCTGC 
CCTGGGGCCT CTGGATTTGC TCCTGCTGGC CGTGTGGCCG AGCCCCTGCC CCTCCGCAGG 
CCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPT ( XM_015474360.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329846.1 Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa92458
Clone ID Related Accession (Same CDS sequence)	XM_015474360.1
Accession Version	XM_015474360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	alanine aminotransferase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCTA TCGCTCCCTG CAGGGCCTGC AGAGGTGGGC CCCGAGGCTT ATCACAGCTC 
CCTTCCCACT CTTTGCACCA ATCAGGAGCC TCAGGGAGGG GGCACTTAGG TCACCTTGCC 
CCTGGTGCAG CCCCAAGGGC AGCTCCACGC GGTGCAGCCC CAAGGGCAGC TCCAGGCTGG 
TACGTGTTTG GCAAAAGGGC AGGACAGCAG CCCAGAGCTG CAGAGGTGTC CCGAGGGCCC 
TGCGAAGGCC CGGAGCTGCC CAGCACCCCC ACTCCCAGCA CCCCAGCTCA GAGACTCCAG 
CCTTGCAGCA CCGATGGGGG ACCAAGCTCC AGACACAGGC TCTTGCTGGC CCAGGCCTGG 
CCCCAGCGCA GGAGGGCCTC GCTCCCCTCC CCCAACAGCC CCAAGATGCC TGAGGCCTGG 
CTCAGTTACC CGGGAGACAG GCCTGCTCTG CTGGCCGGGA GCAAAGATGG GGGGTCCGAG 
AGGGCTGGGC CTCGGCCGAG CCCTACTGGG GTAACTATGC TGAGGTCCTG GGCCTTAGTC 
CCGCTCGCTT GGGGGCCCAG CCCTCCCAGC TCTGTGGACC CCTCAGGGCG CTCACCCCCT 
CCCTGCCCGC AGGCCTGGTC CCACAGAGCT GCTGGCACCT TCTGCCTGTC CCTGCCCCAT 
GTGGGTCTCC TGTCCCCTGT CCTGGGGACG CTGGGCGGGC ACCGGGCCTC ACCCCAAGCC 
CCGCCTGCCT TCTCACCCAC TCTCTGTGGC TCCCAGGGCA GATCTGTGCC CAGGTGGGCG 
GGGCCTGGCA GTCCGGCTCC TTCAGCCTCT TCTGTCCTCT CAGTGGGGCC AGCAGGCTGC 
AGCCAAGAGG GCGCCCCTTC CCAGGCTCCC CCTTCCCAGC CCTGGCCCAT CGAGCCTGGA 
CCGGCTGACC TCACCCCTGC TTCTGGATCC TGCTGCCTCC TGAGCCTCCT TCCTGCCCAT 
CTGAGCAGAG TCATGGCCTT GAGGGCAGGT GAGCACAGCC AGGAGGCAGC AAATGGGCTG 
AAGGAGAAGG TGCTGACACT GGACTCCATG AATCCTTATG TCCGGAGGGT GGAGTATGCG 
GTGCGAGGCC CCATCGTTCA GCGGGCACTG GAGCTGGAGC AGGAGCTGCG CCAGGGCGTA 
AAGAAGCCCT TCACCGAGGT CATCCGCGCC AACATCGGGG ACGCACAGGC CATGGGGCAG 
ATCCCTATCA CCTTCCCGCG CCAGGTCCTG GCCCTCTGCG TCCATCCCGA TCTCCTGAAC 
AGCCCCGACT TCCCCGACGA CGCCAAGAGG AGGGCGGAGC GCATCTTGCA GGCGTGTGGG 
GGCCACAGCC TGGGGGCCTA CAGCATCAGC GCTGGCGTCC AGATGATCCG TGAGGATGTG 
GCGCGGTACA TCGAGCGGCG CGATGGAGGC ATTCCTGCCG ACCCCAATAA CATCTTTCTG 
TCCACGGGGG CCAGCGATGC CATTGTGGTG CAGACCCGCG AGTGCATCGA GGACGTGATC 
CGCTTCGCCT ATGAGGAGAA GCTTTTCCTA TTGGCCGATG AGGTGTACCA AGACAACGTG 
TATGCCGAGA GCTCGCAGTT CCACTCGTTC AAGAAGGTGC TCACGGAGAT GGGGCCGCCG 
TACGCGGCGC AACAGGAGCT CGCCTCCTTC CACTCGATCT CCAAGGGCTA CATGGGCGAG 
TGCGGCTTCC GCGGCGGCTA CGTGGAGGTG GTGAATATGG ACGCTGCGGT GAAGCAGCAG 
ATGCAGAAGC TGCGGAGCGT GCGGCTGTGC CCACCCACCC CGGGCCAGGT CCTGCTCGAC 
GTGGCTGTCA GCCCGCCGGC GCCCTCAGAC CCCTCCTTCC CGCGGTTCCA GGCGCAGGAG 
AGGAGGGCGG TGCTGGCCGA GCTGGCTGCC AAGGCCAAGC TCACGGAGCA GGTCTTCAAC 
GAAGCTCCCG GCATCCGCTG CAATCCGGTG CAGGGCGCCA TGTATTCGTT CCCGCGCGTG 
CAGCTGCCCC CGCGTGCGGT GCAGCGCGCT CAGGTCCGAA GAGCTGGGCC TGGCTCCCGA 
CATGTTCTTC TGCCTGCGCC TCCTAGAGGA GACTGGCATC TGCGTGGTGC CTGGGAGTGG 
CTTTGGACAA CGGGAAGGCA CCTACCACTT TCGGATGACT ATTCTGCCCC CCATGGAGAA 
GCTACGGCCC CTGCTGGAGA AGCTGAGCCA GTTCCATGCG AAGTTCACCC GCGAGTACTC 
CTGAGGACTC GTCAGAGGCC AGGGCCGGCC AGTAGGGACG ACCTCCGACT GAGGGCACTC 
TGCCCTGGGG CCTCTGGATT TGCTCCTGCT GGCCGTGTGG CCGAGCCCCT GCCCCTCCGC 
AGGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329846.1
CDS	980..3328
Translation	MGPIAPCRACRGGPRGLSQLPSHSLHQSGASGRGHLGHLAPGAAPRAAPRGAAPRAAPGWYVFGKRAGQQPRAAEVSRGPCEGPELPSTPTPSTPAQRLQPCSTDGGPSSRHRLLLAQAWPQRRRASLPSPNSPKMPEAWLSYPGDRPALLAGSKDGGSERAGPRPSPTGVTMLRSWALVPLAWGPSPPSSVDPSGRSPPPCPQAWSHRAAGTFCLSLPHVGLLSPVLGTLGGHRASPQAPPAFSPTLCGSQGRSVPRWAGPGSPAPSASSVLSVGPAGCSQEGAPSQAPPSQPWPIEPGPADLTPASGSCCLLSLLPAHLSRVMALRAGEHSQEAANGLKEKVLTLDSMNPYVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQIPITFPRQVLALCVHPDLLNSPDFPDDAKRRAERILQACGGHSLGAYSISAGVQMIREDVARYIERRDGGIPADPNNIFLSTGASDAIVVQTRECIEDVIRFAYEEKLFLLADEVYQDNVYAESSQFHSFKKVLTEMGPPYAAQQELASFHSISKGYMGECGFRGGYVEVVNMDAAVKQQMQKLRSVRLCPPTPGQVLLDVAVSPPAPSDPSFPRFQAQERRAVLAELAAKAKLTEQVFNEAPGIRCNPVQGAMYSFPRVQLPPRAVQRAQVRRAGPGSRHVLLPAPPRGDWHLRGAWEWLWTTGRHLPLSDDYSAPHGEATAPAGEAEPVPCEVHPRVLLRTRQRPGPASRDDLRLRALCPGASGFAPAGRVAEPLPLRRP

Target ORF information:

RefSeq Version	XM_015474360.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474360.1

ORF Insert Sequence:

ATGGGGCCTA TCGCTCCCTG CAGGGCCTGC AGAGGTGGGC CCCGAGGCTT ATCACAGCTC 
CCTTCCCACT CTTTGCACCA ATCAGGAGCC TCAGGGAGGG GGCACTTAGG TCACCTTGCC 
CCTGGTGCAG CCCCAAGGGC AGCTCCACGC GGTGCAGCCC CAAGGGCAGC TCCAGGCTGG 
TACGTGTTTG GCAAAAGGGC AGGACAGCAG CCCAGAGCTG CAGAGGTGTC CCGAGGGCCC 
TGCGAAGGCC CGGAGCTGCC CAGCACCCCC ACTCCCAGCA CCCCAGCTCA GAGACTCCAG 
CCTTGCAGCA CCGATGGGGG ACCAAGCTCC AGACACAGGC TCTTGCTGGC CCAGGCCTGG 
CCCCAGCGCA GGAGGGCCTC GCTCCCCTCC CCCAACAGCC CCAAGATGCC TGAGGCCTGG 
CTCAGTTACC CGGGAGACAG GCCTGCTCTG CTGGCCGGGA GCAAAGATGG GGGGTCCGAG 
AGGGCTGGGC CTCGGCCGAG CCCTACTGGG GTAACTATGC TGAGGTCCTG GGCCTTAGTC 
CCGCTCGCTT GGGGGCCCAG CCCTCCCAGC TCTGTGGACC CCTCAGGGCG CTCACCCCCT 
CCCTGCCCGC AGGCCTGGTC CCACAGAGCT GCTGGCACCT TCTGCCTGTC CCTGCCCCAT 
GTGGGTCTCC TGTCCCCTGT CCTGGGGACG CTGGGCGGGC ACCGGGCCTC ACCCCAAGCC 
CCGCCTGCCT TCTCACCCAC TCTCTGTGGC TCCCAGGGCA GATCTGTGCC CAGGTGGGCG 
GGGCCTGGCA GTCCGGCTCC TTCAGCCTCT TCTGTCCTCT CAGTGGGGCC AGCAGGCTGC 
AGCCAAGAGG GCGCCCCTTC CCAGGCTCCC CCTTCCCAGC CCTGGCCCAT CGAGCCTGGA 
CCGGCTGACC TCACCCCTGC TTCTGGATCC TGCTGCCTCC TGAGCCTCCT TCCTGCCCAT 
CTGAGCAGAG TCATGGCCTT GAGGGCAGGT GAGCACAGCC AGGAGGCAGC AAATGGGCTG 
AAGGAGAAGG TGCTGACACT GGACTCCATG AATCCTTATG TCCGGAGGGT GGAGTATGCG 
GTGCGAGGCC CCATCGTTCA GCGGGCACTG GAGCTGGAGC AGGAGCTGCG CCAGGGCGTA 
AAGAAGCCCT TCACCGAGGT CATCCGCGCC AACATCGGGG ACGCACAGGC CATGGGGCAG 
ATCCCTATCA CCTTCCCGCG CCAGGTCCTG GCCCTCTGCG TCCATCCCGA TCTCCTGAAC 
AGCCCCGACT TCCCCGACGA CGCCAAGAGG AGGGCGGAGC GCATCTTGCA GGCGTGTGGG 
GGCCACAGCC TGGGGGCCTA CAGCATCAGC GCTGGCGTCC AGATGATCCG TGAGGATGTG 
GCGCGGTACA TCGAGCGGCG CGATGGAGGC ATTCCTGCCG ACCCCAATAA CATCTTTCTG 
TCCACGGGGG CCAGCGATGC CATTGTGGTG CAGACCCGCG AGTGCATCGA GGACGTGATC 
CGCTTCGCCT ATGAGGAGAA GCTTTTCCTA TTGGCCGATG AGGTGTACCA AGACAACGTG 
TATGCCGAGA GCTCGCAGTT CCACTCGTTC AAGAAGGTGC TCACGGAGAT GGGGCCGCCG 
TACGCGGCGC AACAGGAGCT CGCCTCCTTC CACTCGATCT CCAAGGGCTA CATGGGCGAG 
TGCGGCTTCC GCGGCGGCTA CGTGGAGGTG GTGAATATGG ACGCTGCGGT GAAGCAGCAG 
ATGCAGAAGC TGCGGAGCGT GCGGCTGTGC CCACCCACCC CGGGCCAGGT CCTGCTCGAC 
GTGGCTGTCA GCCCGCCGGC GCCCTCAGAC CCCTCCTTCC CGCGGTTCCA GGCGCAGGAG 
AGGAGGGCGG TGCTGGCCGA GCTGGCTGCC AAGGCCAAGC TCACGGAGCA GGTCTTCAAC 
GAAGCTCCCG GCATCCGCTG CAATCCGGTG CAGGGCGCCA TGTATTCGTT CCCGCGCGTG 
CAGCTGCCCC CGCGTGCGGT GCAGCGCGCT CAGGTCCGAA GAGCTGGGCC TGGCTCCCGA 
CATGTTCTTC TGCCTGCGCC TCCTAGAGGA GACTGGCATC TGCGTGGTGC CTGGGAGTGG 
CTTTGGACAA CGGGAAGGCA CCTACCACTT TCGGATGACT ATTCTGCCCC CCATGGAGAA 
GCTACGGCCC CTGCTGGAGA AGCTGAGCCA GTTCCATGCG AAGTTCACCC GCGAGTACTC 
CTGAGGACTC GTCAGAGGCC AGGGCCGGCC AGTAGGGACG ACCTCCGACT GAGGGCACTC 
TGCCCTGGGG CCTCTGGATT TGCTCCTGCT GGCCGTGTGG CCGAGCCCCT GCCCCTCCGC 
AGGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glutamine synthetase and alanine transaminase expression are decreased in livers of aged vs. young beef cows and GS can be upregulated by 17?-estradiol implants.
Journal of animal science93(9)4500-9(2015 Sep)
Miles ED,McBride BW,Jia Y,Liao SF,Boling JA,Bridges PJ,Matthews JC

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Assessment of the gene content of the chromosomal regions flanking bovine DGAT1.
Genomics83(1)172-80(2004 Jan)
Winter A,Alzinger A,Fries R

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GPT cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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