MED12L gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005201808.3	XP_005201865.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X4
XM_005201810.3	XP_005201867.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X6
XM_005201810.4	XP_005201867.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X3
XM_024990957.1	XP_024846725.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X6
NM_001206505.1	NP_001193434.1	mediator of RNA polymerase II transcription subunit 12-like protein
XM_024990950.1	XP_024846718.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X2
XM_024990956.1	XP_024846724.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X5
XM_024990959.1	XP_024846727.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X7
XM_010801486.2	XP_010799788.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
XM_010801488.2	XP_010799790.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X3
XM_005201808.4	XP_005201865.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
XM_024990948.1	XP_024846716.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
XM_024990954.1	XP_024846722.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X4
XM_010801485.2	XP_010799787.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
NM_001206505.2	NP_001193434.1	mediator of RNA polymerase II transcription subunit 12-like protein
XM_010801491.2	XP_010799793.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X5
XM_010801487.2	XP_010799789.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa218867	XM_024990959.1 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa218863	NM_001206505.2 Latest version!	Bos taurus mediator complex subunit 12L (MED12L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa218861	XM_005201808.4 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa218861	XM_024990948.1 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa218862	XM_024990950.1 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa218863	XM_005201810.4 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa218864	XM_024990954.1 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa218865	XM_024990956.1 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa218866	XM_024990957.1 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa15948	NM_001206505.1 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa15946	XM_005201808.3 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa15948	XM_005201810.3 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa54150	XM_010801486.2 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa54150	XM_010801485.2 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa54151	XM_010801487.2 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa54152	XM_010801488.2 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa54154	XM_010801491.2 Latest version!	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

MED12L ( XM_024990959.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846727.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa218867
Clone ID Related Accession (Same CDS sequence)	XM_024990959.1
Accession Version	XM_024990959.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAGTCCTG TGAGAATGCC 
AACCCTTCTC TGGGCAGAAG AATGTCAGTT AATGGTGAGA AGCTGTTGAA GAGAGAAAAA 
CCAAGGGAGT TGATTTTTCC ATCTAATTAT GACCTCCTAC GACACCTGCA GTATGCAACC 
CATTTTCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTT 
CTTTATGGAG TGGGAAAAGA GCGTGACGAA GCCAGACATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC AACACAGAGA CAGGGGTTGG AGATGAAGGA 
CAGAAAGCCA GGAAGAGCAA ACAGGAAGCA TTTCCGACCC TGGAGACTGT GTTCACAAAA 
CTTCAACTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATTTC TAACAATGTG 
CTAGAACAAA TCACAAGCTT TGCATCAGGA ACATCATACC ATCTCCCATT GGCTCACCAC 
ATTCAGCTCA TCTTTGATCT CATGGAGCCA GCACTTAATA TTAATGGACT CATTGACTTC 
GCAATACAGC TCCTGAACGA ACTGAGTGTT GTGGAGGCAG AACTGCTCCT GAAGTCCTCC 
AGCCTGGCAG GAAGCTACAC GACAGGACTG TGTGTCTGCA TCGTGGCTGT TCTCAGGCGT 
TACCACAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGATTGTGT 
GGTGTGGTAA AGCACGTTGT GAACCCCTCA GAGTGCTCTT CCCCCGAAAG ATGCATCTTA 
GCCTACCTCT ACGACCTCTA CGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTAGTG CCTGTTCAAA AGTAAAGCAA ACCATATATA ACAATGTGAT TCCTGCAAAT 
TCTAACTTGC GATGGGATCC AGACTTCATG ATGGATTTTA TTGAGAATCC CTCGGCCCGT 
AGCGTCAACT ACTCAGTGCT GGGCAAGATC CTTAGTGACA ATGCAGCCAA TCGCTACAGC 
TTCGTCTGCA ACACTCTCAT GAATGTATGT ATGGGTCATC AGGATGCTGG AAGGATTAAT 
GACATAGCCA ATTTCTCCTC CGAGCTGACA GCCTGCTGCA CTGTTCTCAG TTCAGAATGG 
CTGGGGGTCC TGAAGGCTCT TTGTTGTTCT TCAAATCATG TGTGGGGCTT TAATGATGTT 
CTTTGCACTG TCGATGTGAG TGACCTTTCA TTCCATGATT CATTAGCTAC TTTCATTGCT 
ATTCTGATAG CACGACAGTG TTTCTCCCTG GAGGACGTCG TGCAACACGT CGCACTCCCC 
TCTCTCCTCG CCGCAGCTTG CGGAGATGCA GATGCAGAGC CTGGGGCAAG AATGACATGC 
CGTCTCCTGC TTCATCTCTT TCGAGCTCCC CAGGCCTGCC TGTTACCTCA AGCAACTGGC 
AAACCTTTCC CGGGAATAAG ATCGTCTTGT GACAGACACC TCTTAGCTGC TGCTCACAAC 
AGCATTGAAG TGGGAGCCGT GTTTGCTGTC TTAAAAGCAA TTATGATGCT TGGAGATGCC 
AAAATTGGCA ATAACAGTGT CAGCTCTTTA AAAAATGATG ACTTCACCAT GAGGGGTTTA 
CGACGTGATG GGAATGCTGA GGATATCTGG ACTGCCTCAC AAAATTCAAA ATCCTGTGGG 
AAAAGCATTT CCATAGAAAC GGCCAATTTA AGAGAATATG CTAGATATGT ACTGAGGACC 
ATCTGTCAAC AGGAATGGGT AGGAGAACAT TGTTTAAAAG AACCTGAGAG ATTATGCACA 
GACAAAGAAC TTATTCTGGA CCCTGTGCTC TCAAATATGC AAGCACAGAA ATTACTGCAG 
CTGATCTGTT ATCCTCATGG CATTAAGGAA TGTACGGAGG GGGACAACCT CCAAAGACAG 
CACATTAAAC GCATTCTTCA GAATCTTGAG CAGTGGACGC TGAGGCAGTC CTGGCTGGAG 
CTTCAGTTAA TGATCAAACA GTGCTTGAAG GACCCTGGCT CTGGTTCGGT GGCTGAAATG 
AACAACCTGC TGGACAATAT TGCCAAGGCA ACAATAGAGG TGTTCCAGCA GTCCGCTGAC 
CTAAACAATA ACTCTTCTAA TGCTGGCATG GGCCTCTTCA ACCCAAATGG AATTGGAAGT 
ACTGATACAA GTAGCACGAG ACAGAATGGA ATAAAGACAT TTCTAAGTTC CTCTGAACGC 
AGGGGTGTGT GGCTGGTGGC CCCCCTAATT GCCAGGCTGC CGACTTCTGT GCAGGGAAGA 
GTGTTGAAAG CAGCTGGGGA AGAGCTGGAG AAAGGACAGC ACTTGGGTTC TTCTTCCAAA 
AAGGAAAGAG ACAGACAAAA GCAGAAAAGT ATGTCTCTTT TGAGTCAACA ACCATTCCTC 
TCACTGGTCC TTACCTGCCT TAAGGGACAA GATGAACAAC GAGAAGGCCT TCTAACATCT 
CTGCAGAATC AAGTTAACCA GATTTTAAGT AACTGGAGAG AAGAACGATA CCAGGATGAC 
ATCAAAGCAC GACAGATGAT GCATGAGGCA TTGCAACTCC GCCTGAATTT GGTGGGAGGA 
ATGTTTGACA CAGTGCAGAG GAGCACCCAG TGGACAACAG ACTGGGCGCT TCTCCTCCTT 
CAGATCATTA CTTCTGGAAC CGTTGACATG CACACCAACA ATGAATTATT CACAACAGTT 
CTTGACATGC TGGGTGTTTT AATCAATGGA ACATTGGCCT CTGACCTGTC GAATGCATCC 
CCAGGGGGAT CTGAAGAGAA CAAACGTGCA TACATGAACT TAGTCAAGAA ACTGAAAAAA 
GAGCTGGGGG ACAAGCGGTC AGAAAGTATT GACAAAGTTC GACAGCTGTT ACCTTTGCCG 
AAACAGACGT GTGACATCAT TACCTGTGAA CCTATGGGCT CCTTGATTGA CACAAAGGGA 
AACAAAATTG CTGGATTCGA TTCTATAGAT AAAAAACAGG GTCTCCAGGT CTCCACGAAG 
CAGAAGGTGT CCCCGTGGGA TTTGTTTGAG GGTCAGAAGA ACCCGGCTCC TCTGTCCTGG 
GCCTGGTTCG GGACTGTCCG AGTGGACCGG AAGGTGATCA AGTATGAGGA GCAACATCAC 
CTCCTCCTGT ATCACACGCA CCCCACGCCC AAGCCCCGGA GTTACTACCT CGAGCCACTG 
CCCCTGCCTC CCGAAGAGGA AGAGGAAGAG CCCACATCTC CCATCTCTCA GGAACCTGAG 
AGGAAATCAG CTGAGCTGTC AGATCAGGGA AAAACCGCAA CAGATGAAGA GAAGAAGACA 
AAGGGAAGGA AGCGCAAGAC AAAGTCCAGC TCAAGGGTTG ATGAGTATCC GCAGAGCAGC 
ATGTACCGAG TGCCTCCCAA CTACTCACCC ATCTCCTCCC AGGTGATGCA CCACCCGCAG 
TCCGCCCTGT GGGGCTACAA CCTCATGGGT CAGCCCCAGC AGCCCGGCTT CTTCCTTCAG 
AACCAGTCTG TCACTCCAGG TGGCTCCCGG TTGGACCCCG CTGGCTCCTT CGTCCCCACC 
AACACCAAGC AAGCTTTGTC CAACATGCTC CAGCGGCGCT CGGGGGCCAT GATGCAGCCG 
CCCTCTCTGC ATGCCATCAC ATCGCAGCAG CAGTTGATAC AGATGAAGCT TCTGCAGCAG 
CAGCAGCAAC AGCGGCTTCT CAGGCAAGCC CAGACGCGGC CTTTCCAACA GGGCCAGCCA 
GGGGACCAGG CTGCTCTCTT TGCTGCTCAG GCACGGCCCT CCCCTCAGCT CCCCCAGTAT 
CCAGGACTGC AGCAAGCACA GATAACTTCG AAGAGGAATC ATGGTAGAAT AAGAAGAAAA 
TCGACCTTGG CATCACACCT TGACAAAATT CCTGACAACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846727.1
CDS	414..6338
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQITSKRNHGRIRRKSTLASHLDKIPDNL

Target ORF information:

RefSeq Version	XM_024990959.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990959.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAGTCCTG TGAGAATGCC 
AACCCTTCTC TGGGCAGAAG AATGTCAGTT AATGGTGAGA AGCTGTTGAA GAGAGAAAAA 
CCAAGGGAGT TGATTTTTCC ATCTAATTAT GACCTCCTAC GACACCTGCA GTATGCAACC 
CATTTTCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTT 
CTTTATGGAG TGGGAAAAGA GCGTGACGAA GCCAGACATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC AACACAGAGA CAGGGGTTGG AGATGAAGGA 
CAGAAAGCCA GGAAGAGCAA ACAGGAAGCA TTTCCGACCC TGGAGACTGT GTTCACAAAA 
CTTCAACTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATTTC TAACAATGTG 
CTAGAACAAA TCACAAGCTT TGCATCAGGA ACATCATACC ATCTCCCATT GGCTCACCAC 
ATTCAGCTCA TCTTTGATCT CATGGAGCCA GCACTTAATA TTAATGGACT CATTGACTTC 
GCAATACAGC TCCTGAACGA ACTGAGTGTT GTGGAGGCAG AACTGCTCCT GAAGTCCTCC 
AGCCTGGCAG GAAGCTACAC GACAGGACTG TGTGTCTGCA TCGTGGCTGT TCTCAGGCGT 
TACCACAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGATTGTGT 
GGTGTGGTAA AGCACGTTGT GAACCCCTCA GAGTGCTCTT CCCCCGAAAG ATGCATCTTA 
GCCTACCTCT ACGACCTCTA CGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTAGTG CCTGTTCAAA AGTAAAGCAA ACCATATATA ACAATGTGAT TCCTGCAAAT 
TCTAACTTGC GATGGGATCC AGACTTCATG ATGGATTTTA TTGAGAATCC CTCGGCCCGT 
AGCGTCAACT ACTCAGTGCT GGGCAAGATC CTTAGTGACA ATGCAGCCAA TCGCTACAGC 
TTCGTCTGCA ACACTCTCAT GAATGTATGT ATGGGTCATC AGGATGCTGG AAGGATTAAT 
GACATAGCCA ATTTCTCCTC CGAGCTGACA GCCTGCTGCA CTGTTCTCAG TTCAGAATGG 
CTGGGGGTCC TGAAGGCTCT TTGTTGTTCT TCAAATCATG TGTGGGGCTT TAATGATGTT 
CTTTGCACTG TCGATGTGAG TGACCTTTCA TTCCATGATT CATTAGCTAC TTTCATTGCT 
ATTCTGATAG CACGACAGTG TTTCTCCCTG GAGGACGTCG TGCAACACGT CGCACTCCCC 
TCTCTCCTCG CCGCAGCTTG CGGAGATGCA GATGCAGAGC CTGGGGCAAG AATGACATGC 
CGTCTCCTGC TTCATCTCTT TCGAGCTCCC CAGGCCTGCC TGTTACCTCA AGCAACTGGC 
AAACCTTTCC CGGGAATAAG ATCGTCTTGT GACAGACACC TCTTAGCTGC TGCTCACAAC 
AGCATTGAAG TGGGAGCCGT GTTTGCTGTC TTAAAAGCAA TTATGATGCT TGGAGATGCC 
AAAATTGGCA ATAACAGTGT CAGCTCTTTA AAAAATGATG ACTTCACCAT GAGGGGTTTA 
CGACGTGATG GGAATGCTGA GGATATCTGG ACTGCCTCAC AAAATTCAAA ATCCTGTGGG 
AAAAGCATTT CCATAGAAAC GGCCAATTTA AGAGAATATG CTAGATATGT ACTGAGGACC 
ATCTGTCAAC AGGAATGGGT AGGAGAACAT TGTTTAAAAG AACCTGAGAG ATTATGCACA 
GACAAAGAAC TTATTCTGGA CCCTGTGCTC TCAAATATGC AAGCACAGAA ATTACTGCAG 
CTGATCTGTT ATCCTCATGG CATTAAGGAA TGTACGGAGG GGGACAACCT CCAAAGACAG 
CACATTAAAC GCATTCTTCA GAATCTTGAG CAGTGGACGC TGAGGCAGTC CTGGCTGGAG 
CTTCAGTTAA TGATCAAACA GTGCTTGAAG GACCCTGGCT CTGGTTCGGT GGCTGAAATG 
AACAACCTGC TGGACAATAT TGCCAAGGCA ACAATAGAGG TGTTCCAGCA GTCCGCTGAC 
CTAAACAATA ACTCTTCTAA TGCTGGCATG GGCCTCTTCA ACCCAAATGG AATTGGAAGT 
ACTGATACAA GTAGCACGAG ACAGAATGGA ATAAAGACAT TTCTAAGTTC CTCTGAACGC 
AGGGGTGTGT GGCTGGTGGC CCCCCTAATT GCCAGGCTGC CGACTTCTGT GCAGGGAAGA 
GTGTTGAAAG CAGCTGGGGA AGAGCTGGAG AAAGGACAGC ACTTGGGTTC TTCTTCCAAA 
AAGGAAAGAG ACAGACAAAA GCAGAAAAGT ATGTCTCTTT TGAGTCAACA ACCATTCCTC 
TCACTGGTCC TTACCTGCCT TAAGGGACAA GATGAACAAC GAGAAGGCCT TCTAACATCT 
CTGCAGAATC AAGTTAACCA GATTTTAAGT AACTGGAGAG AAGAACGATA CCAGGATGAC 
ATCAAAGCAC GACAGATGAT GCATGAGGCA TTGCAACTCC GCCTGAATTT GGTGGGAGGA 
ATGTTTGACA CAGTGCAGAG GAGCACCCAG TGGACAACAG ACTGGGCGCT TCTCCTCCTT 
CAGATCATTA CTTCTGGAAC CGTTGACATG CACACCAACA ATGAATTATT CACAACAGTT 
CTTGACATGC TGGGTGTTTT AATCAATGGA ACATTGGCCT CTGACCTGTC GAATGCATCC 
CCAGGGGGAT CTGAAGAGAA CAAACGTGCA TACATGAACT TAGTCAAGAA ACTGAAAAAA 
GAGCTGGGGG ACAAGCGGTC AGAAAGTATT GACAAAGTTC GACAGCTGTT ACCTTTGCCG 
AAACAGACGT GTGACATCAT TACCTGTGAA CCTATGGGCT CCTTGATTGA CACAAAGGGA 
AACAAAATTG CTGGATTCGA TTCTATAGAT AAAAAACAGG GTCTCCAGGT CTCCACGAAG 
CAGAAGGTGT CCCCGTGGGA TTTGTTTGAG GGTCAGAAGA ACCCGGCTCC TCTGTCCTGG 
GCCTGGTTCG GGACTGTCCG AGTGGACCGG AAGGTGATCA AGTATGAGGA GCAACATCAC 
CTCCTCCTGT ATCACACGCA CCCCACGCCC AAGCCCCGGA GTTACTACCT CGAGCCACTG 
CCCCTGCCTC CCGAAGAGGA AGAGGAAGAG CCCACATCTC CCATCTCTCA GGAACCTGAG 
AGGAAATCAG CTGAGCTGTC AGATCAGGGA AAAACCGCAA CAGATGAAGA GAAGAAGACA 
AAGGGAAGGA AGCGCAAGAC AAAGTCCAGC TCAAGGGTTG ATGAGTATCC GCAGAGCAGC 
ATGTACCGAG TGCCTCCCAA CTACTCACCC ATCTCCTCCC AGGTGATGCA CCACCCGCAG 
TCCGCCCTGT GGGGCTACAA CCTCATGGGT CAGCCCCAGC AGCCCGGCTT CTTCCTTCAG 
AACCAGTCTG TCACTCCAGG TGGCTCCCGG TTGGACCCCG CTGGCTCCTT CGTCCCCACC 
AACACCAAGC AAGCTTTGTC CAACATGCTC CAGCGGCGCT CGGGGGCCAT GATGCAGCCG 
CCCTCTCTGC ATGCCATCAC ATCGCAGCAG CAGTTGATAC AGATGAAGCT TCTGCAGCAG 
CAGCAGCAAC AGCGGCTTCT CAGGCAAGCC CAGACGCGGC CTTTCCAACA GGGCCAGCCA 
GGGGACCAGG CTGCTCTCTT TGCTGCTCAG GCACGGCCCT CCCCTCAGCT CCCCCAGTAT 
CCAGGACTGC AGCAAGCACA GATAACTTCG AAGAGGAATC ATGGTAGAAT AAGAAGAAAA 
TCGACCTTGG CATCACACCT TGACAAAATT CCTGACAACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( NM_001206505.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193434.1 Bos taurus mediator complex subunit 12L (MED12L), mRNA.

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CloneID	OBa218863
Clone ID Related Accession (Same CDS sequence)	XM_005201810.4 , NM_001206505.2
Accession Version	NM_001206505.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-16
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000001.1. On May 29, 2018 this sequence version replaced NM_001206505.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CCATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC GAAGTATTCA GTGCCAATGG TTCGAGCAAC ATGGCTGATC 
AAGATGACGT GTGCCTATTA CTCTGCTATT TCTGAAGCGA AAATTAAGAA ACGTCCGGCC 
ACTGATCCCA ATTTGGAGTG GACGCAAATA TCTACCAGGT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCCG ACTTCTACCA CCTGGCTTCC AGCGCGGGCG ATGGCCCTGT CCCTGTGCCA 
CCCGACGTGG AGCAGGCCAT GAGGCAGTGG GAGTACAACG AAAAGTTGGC ATTCCACATG 
TTCCAGGAAG GAATGCTGGA AAAGCATGAA TATTTGACAT GGATATTGGA TGTGTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTAT TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTCCAGTC GGCCTACCTG TCCCGTCGTC TCGCCTATTT CTGTGCCCGG 
CGTCTGGCGT TGCTGCTGAG CGACAGCCCC AGCCTCCTTG CCGCCCACTC GCCCCATGTG 
ATAATAGGCC CAAACAGCTC GAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGGC 
ATGAGCCCTG TGCAGCTGGC CTTCTCCGAC TTTCTTTCCT GTGCACAGCA CGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTTACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTACT CCACAAACGA CAATAAGAGC GCGAACCCGG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGCCT CCCCATGCCG GGAGGAAACA CGGCTTTCAA CCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCG ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AGGTATTGGA TCGACACTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGACAACCA AGAGGTTGCC 
CCCAACGATG AAGCTGTGGT GACGCTGCTG TGTGAATGGG CTGTGAGCTG TAAGCGGTCA 
GGCAAGCACA GGGCGATGGC TGTGGCGAAA CTCCTGGAGA AGAGACAAGC GGAAATCGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCCATTTC CTCAGCCTCC 
CTCGCTGGAT CTAGTTTACC TGTTTTCCAG AATGTTCTTC TAAGGTTTTT AGATACACAG 
GCCCCCTCGT TGTCGGATCC AAATAGCGAA TGTGAGAAGG TGGAGTTTGT CAACCTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGGCATGAC GTCTTCTCCC ACGATGCCTA CATGTGCACC 
CTCATCTCTC GGGGAGACCT GTCTGTCACC GCCTCCACGC GACCGCGGTC GCCAGCAGGA 
GAGAATACGG ATGAGCATTA TCCAAAGGAC CACGACATGA AAATGGAGAT TTTTTCTCCC 
ATGCCTGGAG AGTCCTGTGA GAATGCCAAC CCTTCTCTGG GCAGAAGAAT GTCAGTTAAT 
GGTGAGAAGC TGTTGAAGAG AGAAAAACCA AGGGAGTTGA TTTTTCCATC TAATTATGAC 
CTCCTACGAC ACCTGCAGTA TGCAACCCAT TTTCCCATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTTCTT TATGGAGTGG GAAAAGAGCG TGACGAAGCC 
AGACATCAGC TGAAGAAGAT TACCAAAGAT ATTTTGAAAA TCCTAAATAA GAAGAGCAAC 
ACAGAGACAG GGGTTGGAGA TGAAGGACAG AAAGCCAGGA AGAGCAAACA GGAAGCATTT 
CCGACCCTGG AGACTGTGTT CACAAAACTT CAACTCCTTT CATATTTTGA TCAGCATCAA 
GTGACATCTC AGATTTCTAA CAATGTGCTA GAACAAATCA CAAGCTTTGC ATCAGGAACA 
TCATACCATC TCCCATTGGC TCACCACATT CAGCTCATCT TTGATCTCAT GGAGCCAGCA 
CTTAATATTA ATGGACTCAT TGACTTCGCA ATACAGCTCC TGAACGAACT GAGTGTTGTG 
GAGGCAGAAC TGCTCCTGAA GTCCTCCAGC CTGGCAGGAA GCTACACGAC AGGACTGTGT 
GTCTGCATCG TGGCTGTTCT CAGGCGTTAC CACAGTTGTC TGATCCTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG ATTGTGTGGT GTGGTAAAGC ACGTTGTGAA CCCCTCAGAG 
TGCTCTTCCC CCGAAAGATG CATCTTAGCC TACCTCTACG ACCTCTACGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT AAAGCAAACC 
ATATATAACA ATGTGATTCC TGCAAATTCT AACTTGCGAT GGGATCCAGA CTTCATGATG 
GATTTTATTG AGAATCCCTC GGCCCGTAGC GTCAACTACT CAGTGCTGGG CAAGATCCTT 
AGTGACAATG CAGCCAATCG CTACAGCTTC GTCTGCAACA CTCTCATGAA TGTATGTATG 
GGTCATCAGG ATGCTGGAAG GATTAATGAC ATAGCCAATT TCTCCTCCGA GCTGACAGCC 
TGCTGCACTG TTCTCAGTTC AGAATGGCTG GGGGTCCTGA AGGCTCTTTG TTGTTCTTCA 
AATCATGTGT GGGGCTTTAA TGATGTTCTT TGCACTGTCG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT CTCCCTGGAG 
GACGTCGTGC AACACGTCGC ACTCCCCTCT CTCCTCGCCG CAGCTTGCGG AGATGCAGAT 
GCAGAGCCTG GGGCAAGAAT GACATGCCGT CTCCTGCTTC ATCTCTTTCG AGCTCCCCAG 
GCCTGCCTGT TACCTCAAGC AACTGGCAAA CCTTTCCCGG GAATAAGATC GTCTTGTGAC 
AGACACCTCT TAGCTGCTGC TCACAACAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAGTGTCAG CTCTTTAAAA 
AATGATGACT TCACCATGAG GGGTTTACGA CGTGATGGGA ATGCTGAGGA TATCTGGACT 
GCCTCACAAA ATTCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACGGC CAATTTAAGA 
GAATATGCTA GATATGTACT GAGGACCATC TGTCAACAGG AATGGGTAGG AGAACATTGT 
TTAAAAGAAC CTGAGAGATT ATGCACAGAC AAAGAACTTA TTCTGGACCC TGTGCTCTCA 
AATATGCAAG CACAGAAATT ACTGCAGCTG ATCTGTTATC CTCATGGCAT TAAGGAATGT 
ACGGAGGGGG ACAACCTCCA AAGACAGCAC ATTAAACGCA TTCTTCAGAA TCTTGAGCAG 
TGGACGCTGA GGCAGTCCTG GCTGGAGCTT CAGTTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCGGTGGC TGAAATGAAC AACCTGCTGG ACAATATTGC CAAGGCAACA 
ATAGAGGTGT TCCAGCAGTC CGCTGACCTA AACAATAACT CTTCTAATGC TGGCATGGGC 
CTCTTCAACC CAAATGGAAT TGGAAGTACT GATACAAGTA GCACGAGACA GAATGGAATA 
AAGACATTTC TAAGTTCCTC TGAACGCAGG GGTGTGTGGC TGGTGGCCCC CCTAATTGCC 
AGGCTGCCGA CTTCTGTGCA GGGAAGAGTG TTGAAAGCAG CTGGGGAAGA GCTGGAGAAA 
GGACAGCACT TGGGTTCTTC TTCCAAAAAG GAAAGAGACA GACAAAAGCA GAAAAGTATG 
TCTCTTTTGA GTCAACAACC ATTCCTCTCA CTGGTCCTTA CCTGCCTTAA GGGACAAGAT 
GAACAACGAG AAGGCCTTCT AACATCTCTG CAGAATCAAG TTAACCAGAT TTTAAGTAAC 
TGGAGAGAAG AACGATACCA GGATGACATC AAAGCACGAC AGATGATGCA TGAGGCATTG 
CAACTCCGCC TGAATTTGGT GGGAGGAATG TTTGACACAG TGCAGAGGAG CACCCAGTGG 
ACAACAGACT GGGCGCTTCT CCTCCTTCAG ATCATTACTT CTGGAACCGT TGACATGCAC 
ACCAACAATG AATTATTCAC AACAGTTCTT GACATGCTGG GTGTTTTAAT CAATGGAACA 
TTGGCCTCTG ACCTGTCGAA TGCATCCCCA GGGGGATCTG AAGAGAACAA ACGTGCATAC 
ATGAACTTAG TCAAGAAACT GAAAAAAGAG CTGGGGGACA AGCGGTCAGA AAGTATTGAC 
AAAGTTCGAC AGCTGTTACC TTTGCCGAAA CAGACGTGTG ACATCATTAC CTGTGAACCT 
ATGGGCTCCT TGATTGACAC AAAGGGAAAC AAAATTGCTG GATTCGATTC TATAGATAAA 
AAACAGGGTC TCCAGGTCTC CACGAAGCAG AAGGTGTCCC CGTGGGATTT GTTTGAGGGT 
CAGAAGAACC CGGCTCCTCT GTCCTGGGCC TGGTTCGGGA CTGTCCGAGT GGACCGGAAG 
GTGATCAAGT ATGAGGAGCA ACATCACCTC CTCCTGTATC ACACGCACCC CACGCCCAAG 
CCCCGGAGTT ACTACCTCGA GCCACTGCCC CTGCCTCCCG AAGAGGAAGA GGAAGAGCCC 
ACATCTCCCA TCTCTCAGGA ACCTGAGAGG AAATCAGCTG AGCTGTCAGA TCAGGGAAAA 
ACCGCAACAG ATGAAGAGAA GAAGACAAAG GGAAGGAAGC GCAAGACAAA GTCCAGCTCA 
AGGGTTGATG AGTATCCGCA GAGCAGCATG TACCGAGTGC CTCCCAACTA CTCACCCATC 
TCCTCCCAGG TGATGCACCA CCCGCAGTCC GCCCTGTGGG GCTACAACCT CATGGGTCAG 
CCCCAGCAGC CCGGCTTCTT CCTTCAGAAC CAGTCTGTCA CTCCAGGTGG CTCCCGGTTG 
GACCCCGCTG GCTCCTTCGT CCCCACCAAC ACCAAGCAAG CTTTGTCCAA CATGCTCCAG 
CGGCGCTCGG GGGCCATGAT GCAGCCGCCC TCTCTGCATG CCATCACATC GCAGCAGCAG 
TTGATACAGA TGAAGCTTCT GCAGCAGCAG CAGCAACAGC GGCTTCTCAG GCAAGCCCAG 
ACGCGGCCTT TCCAACAGGG CCAGCCAGGG GACCAGGCTG CTCTCTTTGC TGCTCAGGCA 
CGGCCCTCCC CTCAGCTCCC CCAGTATCCA GGACTGCAGC AAGCACAGAC CATGCCCCAG 
GGCTACACAA TGTATGGAAC CCAGATGCCT TTGCAGCAGA CCCCTCAGCA GCAGGCTGGC 
AGCGTGGTCC TGTCTCCTAC CTATAATTCC AGAACCTATC CGGCCGCTCA CTCCAACCCA 
GCGCTCATGG AAAGACTCAG ACAGATGCAG CAGCCGCCGA GCGGCTATGT TCAGCAGCAG 
GCGTCACCAT ACCTGCATCC CCTGACCGGC TCCCAGAGGC TGAATCATCA GTCGCTTCAG 
CAGAGCCCTC TGGTGGGTGG AGGAATCGAT GCGGTGCTGA CTTCCGCACA TCCAAACCTT 
CCCTCAGTGC CCTTGTCTCA GGACCCGATG AGACCCAGAC AGCCACAGGT TCGACAGCAG 
CAGAGGCTTC TCCAGATGCA GCAGCCCCAG CAGCCCCCCC AGCCCCAGCA GCCTCCCCAG 
CCTCAGGGGT CCTCGCAGAC CCAGAGTCAG ACCCTTGGTC TCCAAACAAT GCAGCCCCAG 
CAGCCTCTGT TCCCCAGGCA AGGCTTGCAG CAGACCCAGC AGCAACAGCA GACGGCTGCC 
CTGGTGCGGC AGCTCCAGAA GCAGCTTTCC AGTAACCAGC CACAGCAAGG AGTGACTCCC 
TACGGGCATC CTTCACACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193434.1
CDS	1..6444
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	NM_001206505.2
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12L (MED12L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206505.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CCATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC GAAGTATTCA GTGCCAATGG TTCGAGCAAC ATGGCTGATC 
AAGATGACGT GTGCCTATTA CTCTGCTATT TCTGAAGCGA AAATTAAGAA ACGTCCGGCC 
ACTGATCCCA ATTTGGAGTG GACGCAAATA TCTACCAGGT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCCG ACTTCTACCA CCTGGCTTCC AGCGCGGGCG ATGGCCCTGT CCCTGTGCCA 
CCCGACGTGG AGCAGGCCAT GAGGCAGTGG GAGTACAACG AAAAGTTGGC ATTCCACATG 
TTCCAGGAAG GAATGCTGGA AAAGCATGAA TATTTGACAT GGATATTGGA TGTGTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTAT TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTCCAGTC GGCCTACCTG TCCCGTCGTC TCGCCTATTT CTGTGCCCGG 
CGTCTGGCGT TGCTGCTGAG CGACAGCCCC AGCCTCCTTG CCGCCCACTC GCCCCATGTG 
ATAATAGGCC CAAACAGCTC GAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGGC 
ATGAGCCCTG TGCAGCTGGC CTTCTCCGAC TTTCTTTCCT GTGCACAGCA CGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTTACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTACT CCACAAACGA CAATAAGAGC GCGAACCCGG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGCCT CCCCATGCCG GGAGGAAACA CGGCTTTCAA CCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCG ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AGGTATTGGA TCGACACTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGACAACCA AGAGGTTGCC 
CCCAACGATG AAGCTGTGGT GACGCTGCTG TGTGAATGGG CTGTGAGCTG TAAGCGGTCA 
GGCAAGCACA GGGCGATGGC TGTGGCGAAA CTCCTGGAGA AGAGACAAGC GGAAATCGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCCATTTC CTCAGCCTCC 
CTCGCTGGAT CTAGTTTACC TGTTTTCCAG AATGTTCTTC TAAGGTTTTT AGATACACAG 
GCCCCCTCGT TGTCGGATCC AAATAGCGAA TGTGAGAAGG TGGAGTTTGT CAACCTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGGCATGAC GTCTTCTCCC ACGATGCCTA CATGTGCACC 
CTCATCTCTC GGGGAGACCT GTCTGTCACC GCCTCCACGC GACCGCGGTC GCCAGCAGGA 
GAGAATACGG ATGAGCATTA TCCAAAGGAC CACGACATGA AAATGGAGAT TTTTTCTCCC 
ATGCCTGGAG AGTCCTGTGA GAATGCCAAC CCTTCTCTGG GCAGAAGAAT GTCAGTTAAT 
GGTGAGAAGC TGTTGAAGAG AGAAAAACCA AGGGAGTTGA TTTTTCCATC TAATTATGAC 
CTCCTACGAC ACCTGCAGTA TGCAACCCAT TTTCCCATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTTCTT TATGGAGTGG GAAAAGAGCG TGACGAAGCC 
AGACATCAGC TGAAGAAGAT TACCAAAGAT ATTTTGAAAA TCCTAAATAA GAAGAGCAAC 
ACAGAGACAG GGGTTGGAGA TGAAGGACAG AAAGCCAGGA AGAGCAAACA GGAAGCATTT 
CCGACCCTGG AGACTGTGTT CACAAAACTT CAACTCCTTT CATATTTTGA TCAGCATCAA 
GTGACATCTC AGATTTCTAA CAATGTGCTA GAACAAATCA CAAGCTTTGC ATCAGGAACA 
TCATACCATC TCCCATTGGC TCACCACATT CAGCTCATCT TTGATCTCAT GGAGCCAGCA 
CTTAATATTA ATGGACTCAT TGACTTCGCA ATACAGCTCC TGAACGAACT GAGTGTTGTG 
GAGGCAGAAC TGCTCCTGAA GTCCTCCAGC CTGGCAGGAA GCTACACGAC AGGACTGTGT 
GTCTGCATCG TGGCTGTTCT CAGGCGTTAC CACAGTTGTC TGATCCTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG ATTGTGTGGT GTGGTAAAGC ACGTTGTGAA CCCCTCAGAG 
TGCTCTTCCC CCGAAAGATG CATCTTAGCC TACCTCTACG ACCTCTACGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT AAAGCAAACC 
ATATATAACA ATGTGATTCC TGCAAATTCT AACTTGCGAT GGGATCCAGA CTTCATGATG 
GATTTTATTG AGAATCCCTC GGCCCGTAGC GTCAACTACT CAGTGCTGGG CAAGATCCTT 
AGTGACAATG CAGCCAATCG CTACAGCTTC GTCTGCAACA CTCTCATGAA TGTATGTATG 
GGTCATCAGG ATGCTGGAAG GATTAATGAC ATAGCCAATT TCTCCTCCGA GCTGACAGCC 
TGCTGCACTG TTCTCAGTTC AGAATGGCTG GGGGTCCTGA AGGCTCTTTG TTGTTCTTCA 
AATCATGTGT GGGGCTTTAA TGATGTTCTT TGCACTGTCG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT CTCCCTGGAG 
GACGTCGTGC AACACGTCGC ACTCCCCTCT CTCCTCGCCG CAGCTTGCGG AGATGCAGAT 
GCAGAGCCTG GGGCAAGAAT GACATGCCGT CTCCTGCTTC ATCTCTTTCG AGCTCCCCAG 
GCCTGCCTGT TACCTCAAGC AACTGGCAAA CCTTTCCCGG GAATAAGATC GTCTTGTGAC 
AGACACCTCT TAGCTGCTGC TCACAACAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAGTGTCAG CTCTTTAAAA 
AATGATGACT TCACCATGAG GGGTTTACGA CGTGATGGGA ATGCTGAGGA TATCTGGACT 
GCCTCACAAA ATTCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACGGC CAATTTAAGA 
GAATATGCTA GATATGTACT GAGGACCATC TGTCAACAGG AATGGGTAGG AGAACATTGT 
TTAAAAGAAC CTGAGAGATT ATGCACAGAC AAAGAACTTA TTCTGGACCC TGTGCTCTCA 
AATATGCAAG CACAGAAATT ACTGCAGCTG ATCTGTTATC CTCATGGCAT TAAGGAATGT 
ACGGAGGGGG ACAACCTCCA AAGACAGCAC ATTAAACGCA TTCTTCAGAA TCTTGAGCAG 
TGGACGCTGA GGCAGTCCTG GCTGGAGCTT CAGTTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCGGTGGC TGAAATGAAC AACCTGCTGG ACAATATTGC CAAGGCAACA 
ATAGAGGTGT TCCAGCAGTC CGCTGACCTA AACAATAACT CTTCTAATGC TGGCATGGGC 
CTCTTCAACC CAAATGGAAT TGGAAGTACT GATACAAGTA GCACGAGACA GAATGGAATA 
AAGACATTTC TAAGTTCCTC TGAACGCAGG GGTGTGTGGC TGGTGGCCCC CCTAATTGCC 
AGGCTGCCGA CTTCTGTGCA GGGAAGAGTG TTGAAAGCAG CTGGGGAAGA GCTGGAGAAA 
GGACAGCACT TGGGTTCTTC TTCCAAAAAG GAAAGAGACA GACAAAAGCA GAAAAGTATG 
TCTCTTTTGA GTCAACAACC ATTCCTCTCA CTGGTCCTTA CCTGCCTTAA GGGACAAGAT 
GAACAACGAG AAGGCCTTCT AACATCTCTG CAGAATCAAG TTAACCAGAT TTTAAGTAAC 
TGGAGAGAAG AACGATACCA GGATGACATC AAAGCACGAC AGATGATGCA TGAGGCATTG 
CAACTCCGCC TGAATTTGGT GGGAGGAATG TTTGACACAG TGCAGAGGAG CACCCAGTGG 
ACAACAGACT GGGCGCTTCT CCTCCTTCAG ATCATTACTT CTGGAACCGT TGACATGCAC 
ACCAACAATG AATTATTCAC AACAGTTCTT GACATGCTGG GTGTTTTAAT CAATGGAACA 
TTGGCCTCTG ACCTGTCGAA TGCATCCCCA GGGGGATCTG AAGAGAACAA ACGTGCATAC 
ATGAACTTAG TCAAGAAACT GAAAAAAGAG CTGGGGGACA AGCGGTCAGA AAGTATTGAC 
AAAGTTCGAC AGCTGTTACC TTTGCCGAAA CAGACGTGTG ACATCATTAC CTGTGAACCT 
ATGGGCTCCT TGATTGACAC AAAGGGAAAC AAAATTGCTG GATTCGATTC TATAGATAAA 
AAACAGGGTC TCCAGGTCTC CACGAAGCAG AAGGTGTCCC CGTGGGATTT GTTTGAGGGT 
CAGAAGAACC CGGCTCCTCT GTCCTGGGCC TGGTTCGGGA CTGTCCGAGT GGACCGGAAG 
GTGATCAAGT ATGAGGAGCA ACATCACCTC CTCCTGTATC ACACGCACCC CACGCCCAAG 
CCCCGGAGTT ACTACCTCGA GCCACTGCCC CTGCCTCCCG AAGAGGAAGA GGAAGAGCCC 
ACATCTCCCA TCTCTCAGGA ACCTGAGAGG AAATCAGCTG AGCTGTCAGA TCAGGGAAAA 
ACCGCAACAG ATGAAGAGAA GAAGACAAAG GGAAGGAAGC GCAAGACAAA GTCCAGCTCA 
AGGGTTGATG AGTATCCGCA GAGCAGCATG TACCGAGTGC CTCCCAACTA CTCACCCATC 
TCCTCCCAGG TGATGCACCA CCCGCAGTCC GCCCTGTGGG GCTACAACCT CATGGGTCAG 
CCCCAGCAGC CCGGCTTCTT CCTTCAGAAC CAGTCTGTCA CTCCAGGTGG CTCCCGGTTG 
GACCCCGCTG GCTCCTTCGT CCCCACCAAC ACCAAGCAAG CTTTGTCCAA CATGCTCCAG 
CGGCGCTCGG GGGCCATGAT GCAGCCGCCC TCTCTGCATG CCATCACATC GCAGCAGCAG 
TTGATACAGA TGAAGCTTCT GCAGCAGCAG CAGCAACAGC GGCTTCTCAG GCAAGCCCAG 
ACGCGGCCTT TCCAACAGGG CCAGCCAGGG GACCAGGCTG CTCTCTTTGC TGCTCAGGCA 
CGGCCCTCCC CTCAGCTCCC CCAGTATCCA GGACTGCAGC AAGCACAGAC CATGCCCCAG 
GGCTACACAA TGTATGGAAC CCAGATGCCT TTGCAGCAGA CCCCTCAGCA GCAGGCTGGC 
AGCGTGGTCC TGTCTCCTAC CTATAATTCC AGAACCTATC CGGCCGCTCA CTCCAACCCA 
GCGCTCATGG AAAGACTCAG ACAGATGCAG CAGCCGCCGA GCGGCTATGT TCAGCAGCAG 
GCGTCACCAT ACCTGCATCC CCTGACCGGC TCCCAGAGGC TGAATCATCA GTCGCTTCAG 
CAGAGCCCTC TGGTGGGTGG AGGAATCGAT GCGGTGCTGA CTTCCGCACA TCCAAACCTT 
CCCTCAGTGC CCTTGTCTCA GGACCCGATG AGACCCAGAC AGCCACAGGT TCGACAGCAG 
CAGAGGCTTC TCCAGATGCA GCAGCCCCAG CAGCCCCCCC AGCCCCAGCA GCCTCCCCAG 
CCTCAGGGGT CCTCGCAGAC CCAGAGTCAG ACCCTTGGTC TCCAAACAAT GCAGCCCCAG 
CAGCCTCTGT TCCCCAGGCA AGGCTTGCAG CAGACCCAGC AGCAACAGCA GACGGCTGCC 
CTGGTGCGGC AGCTCCAGAA GCAGCTTTCC AGTAACCAGC CACAGCAAGG AGTGACTCCC 
TACGGGCATC CTTCACACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_005201808.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201865.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X1, mRNA.

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CloneID	OBa218861
Clone ID Related Accession (Same CDS sequence)	XM_005201808.4 , XM_024990948.1
Accession Version	XM_005201808.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201808.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAGTCCTG TGAGAATGCC 
AACCCTTCTC TGGGCAGAAG AATGTCAGTT AATGGTGAGA AGCTGTTGAA GAGAGAAAAA 
CCAAGGGAGT TGATTTTTCC ATCTAATTAT GACCTCCTAC GACACCTGCA GTATGCAACC 
CATTTTCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTT 
CTTTATGGAG TGGGAAAAGA GCGTGACGAA GCCAGACATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC AACACAGAGA CAGGGGTTGG AGATGAAGGA 
CAGAAAGCCA GGAAGAGCAA ACAGGAAGCA TTTCCGACCC TGGAGACTGT GTTCACAAAA 
CTTCAACTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATTTC TAACAATGTG 
CTAGAACAAA TCACAAGCTT TGCATCAGGA ACATCATACC ATCTCCCATT GGCTCACCAC 
ATTCAGCTCA TCTTTGATCT CATGGAGCCA GCACTTAATA TTAATGGACT CATTGACTTC 
GCAATACAGC TCCTGAACGA ACTGAGTGTT GTGGAGGCAG AACTGCTCCT GAAGTCCTCC 
AGCCTGGCAG GAAGCTACAC GACAGGACTG TGTGTCTGCA TCGTGGCTGT TCTCAGGCGT 
TACCACAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGATTGTGT 
GGTGTGGTAA AGCACGTTGT GAACCCCTCA GAGTGCTCTT CCCCCGAAAG ATGCATCTTA 
GCCTACCTCT ACGACCTCTA CGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTAGTG CCTGTTCAAA AGTAAAGCAA ACCATATATA ACAATGTGAT TCCTGCAAAT 
TCTAACTTGC GATGGGATCC AGACTTCATG ATGGATTTTA TTGAGAATCC CTCGGCCCGT 
AGCGTCAACT ACTCAGTGCT GGGCAAGATC CTTAGTGACA ATGCAGCCAA TCGCTACAGC 
TTCGTCTGCA ACACTCTCAT GAATGTATGT ATGGGTCATC AGGATGCTGG AAGGATTAAT 
GACATAGCCA ATTTCTCCTC CGAGCTGACA GCCTGCTGCA CTGTTCTCAG TTCAGAATGG 
CTGGGGGTCC TGAAGGCTCT TTGTTGTTCT TCAAATCATG TGTGGGGCTT TAATGATGTT 
CTTTGCACTG TCGATGTGAG TGACCTTTCA TTCCATGATT CATTAGCTAC TTTCATTGCT 
ATTCTGATAG CACGACAGTG TTTCTCCCTG GAGGACGTCG TGCAACACGT CGCACTCCCC 
TCTCTCCTCG CCGCAGCTTG CGGAGATGCA GATGCAGAGC CTGGGGCAAG AATGACATGC 
CGTCTCCTGC TTCATCTCTT TCGAGCTCCC CAGGCCTGCC TGTTACCTCA AGCAACTGGC 
AAACCTTTCC CGGGAATAAG ATCGTCTTGT GACAGACACC TCTTAGCTGC TGCTCACAAC 
AGCATTGAAG TGGGAGCCGT GTTTGCTGTC TTAAAAGCAA TTATGATGCT TGGAGATGCC 
AAAATTGGCA ATAACAGTGT CAGCTCTTTA AAAAATGATG ACTTCACCAT GAGGGGTTTA 
CGACGTGATG GGAATGCTGA GGATATCTGG ACTGCCTCAC AAAATTCAAA ATCCTGTGGG 
AAAAGCATTT CCATAGAAAC GGCCAATTTA AGAGAATATG CTAGATATGT ACTGAGGACC 
ATCTGTCAAC AGGAATGGGT AGGAGAACAT TGTTTAAAAG AACCTGAGAG ATTATGCACA 
GACAAAGAAC TTATTCTGGA CCCTGTGCTC TCAAATATGC AAGCACAGAA ATTACTGCAG 
CTGATCTGTT ATCCTCATGG CATTAAGGAA TGTACGGAGG GGGACAACCT CCAAAGACAG 
CACATTAAAC GCATTCTTCA GAATCTTGAG CAGTGGACGC TGAGGCAGTC CTGGCTGGAG 
CTTCAGTTAA TGATCAAACA GTGCTTGAAG GACCCTGGCT CTGGTTCGGT GGCTGAAATG 
AACAACCTGC TGGACAATAT TGCCAAGGCA ACAATAGAGG TGTTCCAGCA GTCCGCTGAC 
CTAAACAATA ACTCTTCTAA TGCTGGCATG GGCCTCTTCA ACCCAAATGG AATTGGAAGT 
ACTGATACAA GTAGCACGAG ACAGAATGGA ATAAAGACAT TTCTAAGTTC CTCTGAACGC 
AGGGGTGTGT GGCTGGTGGC CCCCCTAATT GCCAGGCTGC CGACTTCTGT GCAGGGAAGA 
GTGTTGAAAG CAGCTGGGGA AGAGCTGGAG AAAGGACAGC ACTTGGGTTC TTCTTCCAAA 
AAGGAAAGAG ACAGACAAAA GCAGAAAAGT ATGTCTCTTT TGAGTCAACA ACCATTCCTC 
TCACTGGTCC TTACCTGCCT TAAGGGACAA GATGAACAAC GAGAAGGCCT TCTAACATCT 
CTGCAGAATC AAGTTAACCA GATTTTAAGT AACTGGAGAG AAGAACGATA CCAGGATGAC 
ATCAAAGCAC GACAGATGAT GCATGAGGCA TTGCAACTCC GCCTGAATTT GGTGGGAGGA 
ATGTTTGACA CAGTGCAGAG GAGCACCCAG TGGACAACAG ACTGGGCGCT TCTCCTCCTT 
CAGATCATTA CTTCTGGAAC CGTTGACATG CACACCAACA ATGAATTATT CACAACAGTT 
CTTGACATGC TGGGTGTTTT AATCAATGGA ACATTGGCCT CTGACCTGTC GAATGCATCC 
CCAGGGGGAT CTGAAGAGAA CAAACGTGCA TACATGAACT TAGTCAAGAA ACTGAAAAAA 
GAGCTGGGGG ACAAGCGGTC AGAAAGTATT GACAAAGTTC GACAGCTGTT ACCTTTGCCG 
AAACAGACGT GTGACATCAT TACCTGTGAA CCTATGGGCT CCTTGATTGA CACAAAGGGA 
AACAAAATTG CTGGATTCGA TTCTATAGAT AAAAAACAGG GTCTCCAGGT CTCCACGAAG 
CAGAAGGTGT CCCCGTGGGA TTTGTTTGAG GGTCAGAAGA ACCCGGCTCC TCTGTCCTGG 
GCCTGGTTCG GGACTGTCCG AGTGGACCGG AAGGTGATCA AGTATGAGGA GCAACATCAC 
CTCCTCCTGT ATCACACGCA CCCCACGCCC AAGCCCCGGA GTTACTACCT CGAGCCACTG 
CCCCTGCCTC CCGAAGAGGA AGAGGAAGAG CCCACATCTC CCATCTCTCA GGAACCTGAG 
AGGAAATCAG CTGAGCTGTC AGATCAGGGA AAAACCGCAA CAGATGAAGA GAAGAAGACA 
AAGGGAAGGA AGCGCAAGAC AAAGTCCAGC TCAAGGGTTG ATGAGTATCC GCAGAGCAGC 
ATGTACCGAG TGCCTCCCAA CTACTCACCC ATCTCCTCCC AGGTGATGCA CCACCCGCAG 
TCCGCCCTGT GGGGCTACAA CCTCATGGGT CAGCCCCAGC AGCCCGGCTT CTTCCTTCAG 
AACCAGTCTG TCACTCCAGG TGGCTCCCGG TTGGACCCCG CTGGCTCCTT CGTCCCCACC 
AACACCAAGC AAGCTTTGTC CAACATGCTC CAGCGGCGCT CGGGGGCCAT GATGCAGCCG 
CCCTCTCTGC ATGCCATCAC ATCGCAGCAG CAGTTGATAC AGATGAAGCT TCTGCAGCAG 
CAGCAGCAAC AGCGGCTTCT CAGGCAAGCC CAGACGCGGC CTTTCCAACA GGGCCAGCCA 
GGGGACCAGG CTGCTCTCTT TGCTGCTCAG GCACGGCCCT CCCCTCAGCT CCCCCAGTAT 
CCAGGACTGC AGCAAGCACA GACCATGCCC CAGGGCTACA CAATGTATGG AACCCAGATG 
CCTTTGCAGC AGACCCCTCA GCAGCAGGCT GGCAGCGTGG TCCTGTCTCC TACCTATAAT 
TCCAGAACCT ATCCGGCCGC TCACTCCAAC CCAGCGCTCA TGGAAAGACT CAGACAGATG 
CAGCAGCCGC CGAGCGGCTA TGTTCAGCAG CAGGCGTCAC CATACCTGCA TCCCCTGACC 
GGCTCCCAGA GGCTGAATCA TCAGTCGCTT CAGCAGAGCC CTCTGGTGGG TGGAGGAATC 
GATGCGGTGC TGACTTCCGC ACATCCAAAC CTTCCCTCAG TGCCCTTGTC TCAGGACCCG 
ATGAGACCCA GACAGCCACA GGTTCGACAG CAGCAGAGGC TTCTCCAGAT GCAGCAGCCC 
CAGCAGCCCC CCCAGCCCCA GCAGCCTCCC CAGCCTCAGG GGTCCTCGCA GACCCAGAGT 
CAGACCCTTG GTCTCCAAAC AATGCAGCCC CAGCAGCCTC TGTTCCCCAG GCAAGGCTTG 
CAGCAGACCC AGCAGCAACA GCAGACGGCT GCCCTGGTGC GGCAGCTCCA GAAGCAGCTT 
TCCAGTAACC AGCCACAGCA AGGAGTGACT CCCTACGGGC ATCCTTCACA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201865.1
CDS	414..6890
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_005201808.4
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201808.4

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAGTCCTG TGAGAATGCC 
AACCCTTCTC TGGGCAGAAG AATGTCAGTT AATGGTGAGA AGCTGTTGAA GAGAGAAAAA 
CCAAGGGAGT TGATTTTTCC ATCTAATTAT GACCTCCTAC GACACCTGCA GTATGCAACC 
CATTTTCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTT 
CTTTATGGAG TGGGAAAAGA GCGTGACGAA GCCAGACATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC AACACAGAGA CAGGGGTTGG AGATGAAGGA 
CAGAAAGCCA GGAAGAGCAA ACAGGAAGCA TTTCCGACCC TGGAGACTGT GTTCACAAAA 
CTTCAACTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATTTC TAACAATGTG 
CTAGAACAAA TCACAAGCTT TGCATCAGGA ACATCATACC ATCTCCCATT GGCTCACCAC 
ATTCAGCTCA TCTTTGATCT CATGGAGCCA GCACTTAATA TTAATGGACT CATTGACTTC 
GCAATACAGC TCCTGAACGA ACTGAGTGTT GTGGAGGCAG AACTGCTCCT GAAGTCCTCC 
AGCCTGGCAG GAAGCTACAC GACAGGACTG TGTGTCTGCA TCGTGGCTGT TCTCAGGCGT 
TACCACAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGATTGTGT 
GGTGTGGTAA AGCACGTTGT GAACCCCTCA GAGTGCTCTT CCCCCGAAAG ATGCATCTTA 
GCCTACCTCT ACGACCTCTA CGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTAGTG CCTGTTCAAA AGTAAAGCAA ACCATATATA ACAATGTGAT TCCTGCAAAT 
TCTAACTTGC GATGGGATCC AGACTTCATG ATGGATTTTA TTGAGAATCC CTCGGCCCGT 
AGCGTCAACT ACTCAGTGCT GGGCAAGATC CTTAGTGACA ATGCAGCCAA TCGCTACAGC 
TTCGTCTGCA ACACTCTCAT GAATGTATGT ATGGGTCATC AGGATGCTGG AAGGATTAAT 
GACATAGCCA ATTTCTCCTC CGAGCTGACA GCCTGCTGCA CTGTTCTCAG TTCAGAATGG 
CTGGGGGTCC TGAAGGCTCT TTGTTGTTCT TCAAATCATG TGTGGGGCTT TAATGATGTT 
CTTTGCACTG TCGATGTGAG TGACCTTTCA TTCCATGATT CATTAGCTAC TTTCATTGCT 
ATTCTGATAG CACGACAGTG TTTCTCCCTG GAGGACGTCG TGCAACACGT CGCACTCCCC 
TCTCTCCTCG CCGCAGCTTG CGGAGATGCA GATGCAGAGC CTGGGGCAAG AATGACATGC 
CGTCTCCTGC TTCATCTCTT TCGAGCTCCC CAGGCCTGCC TGTTACCTCA AGCAACTGGC 
AAACCTTTCC CGGGAATAAG ATCGTCTTGT GACAGACACC TCTTAGCTGC TGCTCACAAC 
AGCATTGAAG TGGGAGCCGT GTTTGCTGTC TTAAAAGCAA TTATGATGCT TGGAGATGCC 
AAAATTGGCA ATAACAGTGT CAGCTCTTTA AAAAATGATG ACTTCACCAT GAGGGGTTTA 
CGACGTGATG GGAATGCTGA GGATATCTGG ACTGCCTCAC AAAATTCAAA ATCCTGTGGG 
AAAAGCATTT CCATAGAAAC GGCCAATTTA AGAGAATATG CTAGATATGT ACTGAGGACC 
ATCTGTCAAC AGGAATGGGT AGGAGAACAT TGTTTAAAAG AACCTGAGAG ATTATGCACA 
GACAAAGAAC TTATTCTGGA CCCTGTGCTC TCAAATATGC AAGCACAGAA ATTACTGCAG 
CTGATCTGTT ATCCTCATGG CATTAAGGAA TGTACGGAGG GGGACAACCT CCAAAGACAG 
CACATTAAAC GCATTCTTCA GAATCTTGAG CAGTGGACGC TGAGGCAGTC CTGGCTGGAG 
CTTCAGTTAA TGATCAAACA GTGCTTGAAG GACCCTGGCT CTGGTTCGGT GGCTGAAATG 
AACAACCTGC TGGACAATAT TGCCAAGGCA ACAATAGAGG TGTTCCAGCA GTCCGCTGAC 
CTAAACAATA ACTCTTCTAA TGCTGGCATG GGCCTCTTCA ACCCAAATGG AATTGGAAGT 
ACTGATACAA GTAGCACGAG ACAGAATGGA ATAAAGACAT TTCTAAGTTC CTCTGAACGC 
AGGGGTGTGT GGCTGGTGGC CCCCCTAATT GCCAGGCTGC CGACTTCTGT GCAGGGAAGA 
GTGTTGAAAG CAGCTGGGGA AGAGCTGGAG AAAGGACAGC ACTTGGGTTC TTCTTCCAAA 
AAGGAAAGAG ACAGACAAAA GCAGAAAAGT ATGTCTCTTT TGAGTCAACA ACCATTCCTC 
TCACTGGTCC TTACCTGCCT TAAGGGACAA GATGAACAAC GAGAAGGCCT TCTAACATCT 
CTGCAGAATC AAGTTAACCA GATTTTAAGT AACTGGAGAG AAGAACGATA CCAGGATGAC 
ATCAAAGCAC GACAGATGAT GCATGAGGCA TTGCAACTCC GCCTGAATTT GGTGGGAGGA 
ATGTTTGACA CAGTGCAGAG GAGCACCCAG TGGACAACAG ACTGGGCGCT TCTCCTCCTT 
CAGATCATTA CTTCTGGAAC CGTTGACATG CACACCAACA ATGAATTATT CACAACAGTT 
CTTGACATGC TGGGTGTTTT AATCAATGGA ACATTGGCCT CTGACCTGTC GAATGCATCC 
CCAGGGGGAT CTGAAGAGAA CAAACGTGCA TACATGAACT TAGTCAAGAA ACTGAAAAAA 
GAGCTGGGGG ACAAGCGGTC AGAAAGTATT GACAAAGTTC GACAGCTGTT ACCTTTGCCG 
AAACAGACGT GTGACATCAT TACCTGTGAA CCTATGGGCT CCTTGATTGA CACAAAGGGA 
AACAAAATTG CTGGATTCGA TTCTATAGAT AAAAAACAGG GTCTCCAGGT CTCCACGAAG 
CAGAAGGTGT CCCCGTGGGA TTTGTTTGAG GGTCAGAAGA ACCCGGCTCC TCTGTCCTGG 
GCCTGGTTCG GGACTGTCCG AGTGGACCGG AAGGTGATCA AGTATGAGGA GCAACATCAC 
CTCCTCCTGT ATCACACGCA CCCCACGCCC AAGCCCCGGA GTTACTACCT CGAGCCACTG 
CCCCTGCCTC CCGAAGAGGA AGAGGAAGAG CCCACATCTC CCATCTCTCA GGAACCTGAG 
AGGAAATCAG CTGAGCTGTC AGATCAGGGA AAAACCGCAA CAGATGAAGA GAAGAAGACA 
AAGGGAAGGA AGCGCAAGAC AAAGTCCAGC TCAAGGGTTG ATGAGTATCC GCAGAGCAGC 
ATGTACCGAG TGCCTCCCAA CTACTCACCC ATCTCCTCCC AGGTGATGCA CCACCCGCAG 
TCCGCCCTGT GGGGCTACAA CCTCATGGGT CAGCCCCAGC AGCCCGGCTT CTTCCTTCAG 
AACCAGTCTG TCACTCCAGG TGGCTCCCGG TTGGACCCCG CTGGCTCCTT CGTCCCCACC 
AACACCAAGC AAGCTTTGTC CAACATGCTC CAGCGGCGCT CGGGGGCCAT GATGCAGCCG 
CCCTCTCTGC ATGCCATCAC ATCGCAGCAG CAGTTGATAC AGATGAAGCT TCTGCAGCAG 
CAGCAGCAAC AGCGGCTTCT CAGGCAAGCC CAGACGCGGC CTTTCCAACA GGGCCAGCCA 
GGGGACCAGG CTGCTCTCTT TGCTGCTCAG GCACGGCCCT CCCCTCAGCT CCCCCAGTAT 
CCAGGACTGC AGCAAGCACA GACCATGCCC CAGGGCTACA CAATGTATGG AACCCAGATG 
CCTTTGCAGC AGACCCCTCA GCAGCAGGCT GGCAGCGTGG TCCTGTCTCC TACCTATAAT 
TCCAGAACCT ATCCGGCCGC TCACTCCAAC CCAGCGCTCA TGGAAAGACT CAGACAGATG 
CAGCAGCCGC CGAGCGGCTA TGTTCAGCAG CAGGCGTCAC CATACCTGCA TCCCCTGACC 
GGCTCCCAGA GGCTGAATCA TCAGTCGCTT CAGCAGAGCC CTCTGGTGGG TGGAGGAATC 
GATGCGGTGC TGACTTCCGC ACATCCAAAC CTTCCCTCAG TGCCCTTGTC TCAGGACCCG 
ATGAGACCCA GACAGCCACA GGTTCGACAG CAGCAGAGGC TTCTCCAGAT GCAGCAGCCC 
CAGCAGCCCC CCCAGCCCCA GCAGCCTCCC CAGCCTCAGG GGTCCTCGCA GACCCAGAGT 
CAGACCCTTG GTCTCCAAAC AATGCAGCCC CAGCAGCCTC TGTTCCCCAG GCAAGGCTTG 
CAGCAGACCC AGCAGCAACA GCAGACGGCT GCCCTGGTGC GGCAGCTCCA GAAGCAGCTT 
TCCAGTAACC AGCCACAGCA AGGAGTGACT CCCTACGGGC ATCCTTCACA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_024990948.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846716.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X2, mRNA.

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CloneID	OBa218861
Clone ID Related Accession (Same CDS sequence)	XM_005201808.4 , XM_024990948.1
Accession Version	XM_024990948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAGTCCTG TGAGAATGCC 
AACCCTTCTC TGGGCAGAAG AATGTCAGTT AATGGTGAGA AGCTGTTGAA GAGAGAAAAA 
CCAAGGGAGT TGATTTTTCC ATCTAATTAT GACCTCCTAC GACACCTGCA GTATGCAACC 
CATTTTCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTT 
CTTTATGGAG TGGGAAAAGA GCGTGACGAA GCCAGACATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC AACACAGAGA CAGGGGTTGG AGATGAAGGA 
CAGAAAGCCA GGAAGAGCAA ACAGGAAGCA TTTCCGACCC TGGAGACTGT GTTCACAAAA 
CTTCAACTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATTTC TAACAATGTG 
CTAGAACAAA TCACAAGCTT TGCATCAGGA ACATCATACC ATCTCCCATT GGCTCACCAC 
ATTCAGCTCA TCTTTGATCT CATGGAGCCA GCACTTAATA TTAATGGACT CATTGACTTC 
GCAATACAGC TCCTGAACGA ACTGAGTGTT GTGGAGGCAG AACTGCTCCT GAAGTCCTCC 
AGCCTGGCAG GAAGCTACAC GACAGGACTG TGTGTCTGCA TCGTGGCTGT TCTCAGGCGT 
TACCACAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGATTGTGT 
GGTGTGGTAA AGCACGTTGT GAACCCCTCA GAGTGCTCTT CCCCCGAAAG ATGCATCTTA 
GCCTACCTCT ACGACCTCTA CGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTAGTG CCTGTTCAAA AGTAAAGCAA ACCATATATA ACAATGTGAT TCCTGCAAAT 
TCTAACTTGC GATGGGATCC AGACTTCATG ATGGATTTTA TTGAGAATCC CTCGGCCCGT 
AGCGTCAACT ACTCAGTGCT GGGCAAGATC CTTAGTGACA ATGCAGCCAA TCGCTACAGC 
TTCGTCTGCA ACACTCTCAT GAATGTATGT ATGGGTCATC AGGATGCTGG AAGGATTAAT 
GACATAGCCA ATTTCTCCTC CGAGCTGACA GCCTGCTGCA CTGTTCTCAG TTCAGAATGG 
CTGGGGGTCC TGAAGGCTCT TTGTTGTTCT TCAAATCATG TGTGGGGCTT TAATGATGTT 
CTTTGCACTG TCGATGTGAG TGACCTTTCA TTCCATGATT CATTAGCTAC TTTCATTGCT 
ATTCTGATAG CACGACAGTG TTTCTCCCTG GAGGACGTCG TGCAACACGT CGCACTCCCC 
TCTCTCCTCG CCGCAGCTTG CGGAGATGCA GATGCAGAGC CTGGGGCAAG AATGACATGC 
CGTCTCCTGC TTCATCTCTT TCGAGCTCCC CAGGCCTGCC TGTTACCTCA AGCAACTGGC 
AAACCTTTCC CGGGAATAAG ATCGTCTTGT GACAGACACC TCTTAGCTGC TGCTCACAAC 
AGCATTGAAG TGGGAGCCGT GTTTGCTGTC TTAAAAGCAA TTATGATGCT TGGAGATGCC 
AAAATTGGCA ATAACAGTGT CAGCTCTTTA AAAAATGATG ACTTCACCAT GAGGGGTTTA 
CGACGTGATG GGAATGCTGA GGATATCTGG ACTGCCTCAC AAAATTCAAA ATCCTGTGGG 
AAAAGCATTT CCATAGAAAC GGCCAATTTA AGAGAATATG CTAGATATGT ACTGAGGACC 
ATCTGTCAAC AGGAATGGGT AGGAGAACAT TGTTTAAAAG AACCTGAGAG ATTATGCACA 
GACAAAGAAC TTATTCTGGA CCCTGTGCTC TCAAATATGC AAGCACAGAA ATTACTGCAG 
CTGATCTGTT ATCCTCATGG CATTAAGGAA TGTACGGAGG GGGACAACCT CCAAAGACAG 
CACATTAAAC GCATTCTTCA GAATCTTGAG CAGTGGACGC TGAGGCAGTC CTGGCTGGAG 
CTTCAGTTAA TGATCAAACA GTGCTTGAAG GACCCTGGCT CTGGTTCGGT GGCTGAAATG 
AACAACCTGC TGGACAATAT TGCCAAGGCA ACAATAGAGG TGTTCCAGCA GTCCGCTGAC 
CTAAACAATA ACTCTTCTAA TGCTGGCATG GGCCTCTTCA ACCCAAATGG AATTGGAAGT 
ACTGATACAA GTAGCACGAG ACAGAATGGA ATAAAGACAT TTCTAAGTTC CTCTGAACGC 
AGGGGTGTGT GGCTGGTGGC CCCCCTAATT GCCAGGCTGC CGACTTCTGT GCAGGGAAGA 
GTGTTGAAAG CAGCTGGGGA AGAGCTGGAG AAAGGACAGC ACTTGGGTTC TTCTTCCAAA 
AAGGAAAGAG ACAGACAAAA GCAGAAAAGT ATGTCTCTTT TGAGTCAACA ACCATTCCTC 
TCACTGGTCC TTACCTGCCT TAAGGGACAA GATGAACAAC GAGAAGGCCT TCTAACATCT 
CTGCAGAATC AAGTTAACCA GATTTTAAGT AACTGGAGAG AAGAACGATA CCAGGATGAC 
ATCAAAGCAC GACAGATGAT GCATGAGGCA TTGCAACTCC GCCTGAATTT GGTGGGAGGA 
ATGTTTGACA CAGTGCAGAG GAGCACCCAG TGGACAACAG ACTGGGCGCT TCTCCTCCTT 
CAGATCATTA CTTCTGGAAC CGTTGACATG CACACCAACA ATGAATTATT CACAACAGTT 
CTTGACATGC TGGGTGTTTT AATCAATGGA ACATTGGCCT CTGACCTGTC GAATGCATCC 
CCAGGGGGAT CTGAAGAGAA CAAACGTGCA TACATGAACT TAGTCAAGAA ACTGAAAAAA 
GAGCTGGGGG ACAAGCGGTC AGAAAGTATT GACAAAGTTC GACAGCTGTT ACCTTTGCCG 
AAACAGACGT GTGACATCAT TACCTGTGAA CCTATGGGCT CCTTGATTGA CACAAAGGGA 
AACAAAATTG CTGGATTCGA TTCTATAGAT AAAAAACAGG GTCTCCAGGT CTCCACGAAG 
CAGAAGGTGT CCCCGTGGGA TTTGTTTGAG GGTCAGAAGA ACCCGGCTCC TCTGTCCTGG 
GCCTGGTTCG GGACTGTCCG AGTGGACCGG AAGGTGATCA AGTATGAGGA GCAACATCAC 
CTCCTCCTGT ATCACACGCA CCCCACGCCC AAGCCCCGGA GTTACTACCT CGAGCCACTG 
CCCCTGCCTC CCGAAGAGGA AGAGGAAGAG CCCACATCTC CCATCTCTCA GGAACCTGAG 
AGGAAATCAG CTGAGCTGTC AGATCAGGGA AAAACCGCAA CAGATGAAGA GAAGAAGACA 
AAGGGAAGGA AGCGCAAGAC AAAGTCCAGC TCAAGGGTTG ATGAGTATCC GCAGAGCAGC 
ATGTACCGAG TGCCTCCCAA CTACTCACCC ATCTCCTCCC AGGTGATGCA CCACCCGCAG 
TCCGCCCTGT GGGGCTACAA CCTCATGGGT CAGCCCCAGC AGCCCGGCTT CTTCCTTCAG 
AACCAGTCTG TCACTCCAGG TGGCTCCCGG TTGGACCCCG CTGGCTCCTT CGTCCCCACC 
AACACCAAGC AAGCTTTGTC CAACATGCTC CAGCGGCGCT CGGGGGCCAT GATGCAGCCG 
CCCTCTCTGC ATGCCATCAC ATCGCAGCAG CAGTTGATAC AGATGAAGCT TCTGCAGCAG 
CAGCAGCAAC AGCGGCTTCT CAGGCAAGCC CAGACGCGGC CTTTCCAACA GGGCCAGCCA 
GGGGACCAGG CTGCTCTCTT TGCTGCTCAG GCACGGCCCT CCCCTCAGCT CCCCCAGTAT 
CCAGGACTGC AGCAAGCACA GACCATGCCC CAGGGCTACA CAATGTATGG AACCCAGATG 
CCTTTGCAGC AGACCCCTCA GCAGCAGGCT GGCAGCGTGG TCCTGTCTCC TACCTATAAT 
TCCAGAACCT ATCCGGCCGC TCACTCCAAC CCAGCGCTCA TGGAAAGACT CAGACAGATG 
CAGCAGCCGC CGAGCGGCTA TGTTCAGCAG CAGGCGTCAC CATACCTGCA TCCCCTGACC 
GGCTCCCAGA GGCTGAATCA TCAGTCGCTT CAGCAGAGCC CTCTGGTGGG TGGAGGAATC 
GATGCGGTGC TGACTTCCGC ACATCCAAAC CTTCCCTCAG TGCCCTTGTC TCAGGACCCG 
ATGAGACCCA GACAGCCACA GGTTCGACAG CAGCAGAGGC TTCTCCAGAT GCAGCAGCCC 
CAGCAGCCCC CCCAGCCCCA GCAGCCTCCC CAGCCTCAGG GGTCCTCGCA GACCCAGAGT 
CAGACCCTTG GTCTCCAAAC AATGCAGCCC CAGCAGCCTC TGTTCCCCAG GCAAGGCTTG 
CAGCAGACCC AGCAGCAACA GCAGACGGCT GCCCTGGTGC GGCAGCTCCA GAAGCAGCTT 
TCCAGTAACC AGCCACAGCA AGGAGTGACT CCCTACGGGC ATCCTTCACA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846716.1
CDS	260..6736
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_024990948.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990948.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAGTCCTG TGAGAATGCC 
AACCCTTCTC TGGGCAGAAG AATGTCAGTT AATGGTGAGA AGCTGTTGAA GAGAGAAAAA 
CCAAGGGAGT TGATTTTTCC ATCTAATTAT GACCTCCTAC GACACCTGCA GTATGCAACC 
CATTTTCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTT 
CTTTATGGAG TGGGAAAAGA GCGTGACGAA GCCAGACATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC AACACAGAGA CAGGGGTTGG AGATGAAGGA 
CAGAAAGCCA GGAAGAGCAA ACAGGAAGCA TTTCCGACCC TGGAGACTGT GTTCACAAAA 
CTTCAACTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATTTC TAACAATGTG 
CTAGAACAAA TCACAAGCTT TGCATCAGGA ACATCATACC ATCTCCCATT GGCTCACCAC 
ATTCAGCTCA TCTTTGATCT CATGGAGCCA GCACTTAATA TTAATGGACT CATTGACTTC 
GCAATACAGC TCCTGAACGA ACTGAGTGTT GTGGAGGCAG AACTGCTCCT GAAGTCCTCC 
AGCCTGGCAG GAAGCTACAC GACAGGACTG TGTGTCTGCA TCGTGGCTGT TCTCAGGCGT 
TACCACAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGATTGTGT 
GGTGTGGTAA AGCACGTTGT GAACCCCTCA GAGTGCTCTT CCCCCGAAAG ATGCATCTTA 
GCCTACCTCT ACGACCTCTA CGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTAGTG CCTGTTCAAA AGTAAAGCAA ACCATATATA ACAATGTGAT TCCTGCAAAT 
TCTAACTTGC GATGGGATCC AGACTTCATG ATGGATTTTA TTGAGAATCC CTCGGCCCGT 
AGCGTCAACT ACTCAGTGCT GGGCAAGATC CTTAGTGACA ATGCAGCCAA TCGCTACAGC 
TTCGTCTGCA ACACTCTCAT GAATGTATGT ATGGGTCATC AGGATGCTGG AAGGATTAAT 
GACATAGCCA ATTTCTCCTC CGAGCTGACA GCCTGCTGCA CTGTTCTCAG TTCAGAATGG 
CTGGGGGTCC TGAAGGCTCT TTGTTGTTCT TCAAATCATG TGTGGGGCTT TAATGATGTT 
CTTTGCACTG TCGATGTGAG TGACCTTTCA TTCCATGATT CATTAGCTAC TTTCATTGCT 
ATTCTGATAG CACGACAGTG TTTCTCCCTG GAGGACGTCG TGCAACACGT CGCACTCCCC 
TCTCTCCTCG CCGCAGCTTG CGGAGATGCA GATGCAGAGC CTGGGGCAAG AATGACATGC 
CGTCTCCTGC TTCATCTCTT TCGAGCTCCC CAGGCCTGCC TGTTACCTCA AGCAACTGGC 
AAACCTTTCC CGGGAATAAG ATCGTCTTGT GACAGACACC TCTTAGCTGC TGCTCACAAC 
AGCATTGAAG TGGGAGCCGT GTTTGCTGTC TTAAAAGCAA TTATGATGCT TGGAGATGCC 
AAAATTGGCA ATAACAGTGT CAGCTCTTTA AAAAATGATG ACTTCACCAT GAGGGGTTTA 
CGACGTGATG GGAATGCTGA GGATATCTGG ACTGCCTCAC AAAATTCAAA ATCCTGTGGG 
AAAAGCATTT CCATAGAAAC GGCCAATTTA AGAGAATATG CTAGATATGT ACTGAGGACC 
ATCTGTCAAC AGGAATGGGT AGGAGAACAT TGTTTAAAAG AACCTGAGAG ATTATGCACA 
GACAAAGAAC TTATTCTGGA CCCTGTGCTC TCAAATATGC AAGCACAGAA ATTACTGCAG 
CTGATCTGTT ATCCTCATGG CATTAAGGAA TGTACGGAGG GGGACAACCT CCAAAGACAG 
CACATTAAAC GCATTCTTCA GAATCTTGAG CAGTGGACGC TGAGGCAGTC CTGGCTGGAG 
CTTCAGTTAA TGATCAAACA GTGCTTGAAG GACCCTGGCT CTGGTTCGGT GGCTGAAATG 
AACAACCTGC TGGACAATAT TGCCAAGGCA ACAATAGAGG TGTTCCAGCA GTCCGCTGAC 
CTAAACAATA ACTCTTCTAA TGCTGGCATG GGCCTCTTCA ACCCAAATGG AATTGGAAGT 
ACTGATACAA GTAGCACGAG ACAGAATGGA ATAAAGACAT TTCTAAGTTC CTCTGAACGC 
AGGGGTGTGT GGCTGGTGGC CCCCCTAATT GCCAGGCTGC CGACTTCTGT GCAGGGAAGA 
GTGTTGAAAG CAGCTGGGGA AGAGCTGGAG AAAGGACAGC ACTTGGGTTC TTCTTCCAAA 
AAGGAAAGAG ACAGACAAAA GCAGAAAAGT ATGTCTCTTT TGAGTCAACA ACCATTCCTC 
TCACTGGTCC TTACCTGCCT TAAGGGACAA GATGAACAAC GAGAAGGCCT TCTAACATCT 
CTGCAGAATC AAGTTAACCA GATTTTAAGT AACTGGAGAG AAGAACGATA CCAGGATGAC 
ATCAAAGCAC GACAGATGAT GCATGAGGCA TTGCAACTCC GCCTGAATTT GGTGGGAGGA 
ATGTTTGACA CAGTGCAGAG GAGCACCCAG TGGACAACAG ACTGGGCGCT TCTCCTCCTT 
CAGATCATTA CTTCTGGAAC CGTTGACATG CACACCAACA ATGAATTATT CACAACAGTT 
CTTGACATGC TGGGTGTTTT AATCAATGGA ACATTGGCCT CTGACCTGTC GAATGCATCC 
CCAGGGGGAT CTGAAGAGAA CAAACGTGCA TACATGAACT TAGTCAAGAA ACTGAAAAAA 
GAGCTGGGGG ACAAGCGGTC AGAAAGTATT GACAAAGTTC GACAGCTGTT ACCTTTGCCG 
AAACAGACGT GTGACATCAT TACCTGTGAA CCTATGGGCT CCTTGATTGA CACAAAGGGA 
AACAAAATTG CTGGATTCGA TTCTATAGAT AAAAAACAGG GTCTCCAGGT CTCCACGAAG 
CAGAAGGTGT CCCCGTGGGA TTTGTTTGAG GGTCAGAAGA ACCCGGCTCC TCTGTCCTGG 
GCCTGGTTCG GGACTGTCCG AGTGGACCGG AAGGTGATCA AGTATGAGGA GCAACATCAC 
CTCCTCCTGT ATCACACGCA CCCCACGCCC AAGCCCCGGA GTTACTACCT CGAGCCACTG 
CCCCTGCCTC CCGAAGAGGA AGAGGAAGAG CCCACATCTC CCATCTCTCA GGAACCTGAG 
AGGAAATCAG CTGAGCTGTC AGATCAGGGA AAAACCGCAA CAGATGAAGA GAAGAAGACA 
AAGGGAAGGA AGCGCAAGAC AAAGTCCAGC TCAAGGGTTG ATGAGTATCC GCAGAGCAGC 
ATGTACCGAG TGCCTCCCAA CTACTCACCC ATCTCCTCCC AGGTGATGCA CCACCCGCAG 
TCCGCCCTGT GGGGCTACAA CCTCATGGGT CAGCCCCAGC AGCCCGGCTT CTTCCTTCAG 
AACCAGTCTG TCACTCCAGG TGGCTCCCGG TTGGACCCCG CTGGCTCCTT CGTCCCCACC 
AACACCAAGC AAGCTTTGTC CAACATGCTC CAGCGGCGCT CGGGGGCCAT GATGCAGCCG 
CCCTCTCTGC ATGCCATCAC ATCGCAGCAG CAGTTGATAC AGATGAAGCT TCTGCAGCAG 
CAGCAGCAAC AGCGGCTTCT CAGGCAAGCC CAGACGCGGC CTTTCCAACA GGGCCAGCCA 
GGGGACCAGG CTGCTCTCTT TGCTGCTCAG GCACGGCCCT CCCCTCAGCT CCCCCAGTAT 
CCAGGACTGC AGCAAGCACA GACCATGCCC CAGGGCTACA CAATGTATGG AACCCAGATG 
CCTTTGCAGC AGACCCCTCA GCAGCAGGCT GGCAGCGTGG TCCTGTCTCC TACCTATAAT 
TCCAGAACCT ATCCGGCCGC TCACTCCAAC CCAGCGCTCA TGGAAAGACT CAGACAGATG 
CAGCAGCCGC CGAGCGGCTA TGTTCAGCAG CAGGCGTCAC CATACCTGCA TCCCCTGACC 
GGCTCCCAGA GGCTGAATCA TCAGTCGCTT CAGCAGAGCC CTCTGGTGGG TGGAGGAATC 
GATGCGGTGC TGACTTCCGC ACATCCAAAC CTTCCCTCAG TGCCCTTGTC TCAGGACCCG 
ATGAGACCCA GACAGCCACA GGTTCGACAG CAGCAGAGGC TTCTCCAGAT GCAGCAGCCC 
CAGCAGCCCC CCCAGCCCCA GCAGCCTCCC CAGCCTCAGG GGTCCTCGCA GACCCAGAGT 
CAGACCCTTG GTCTCCAAAC AATGCAGCCC CAGCAGCCTC TGTTCCCCAG GCAAGGCTTG 
CAGCAGACCC AGCAGCAACA GCAGACGGCT GCCCTGGTGC GGCAGCTCCA GAAGCAGCTT 
TCCAGTAACC AGCCACAGCA AGGAGTGACT CCCTACGGGC ATCCTTCACA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_024990950.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846718.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X4, mRNA.

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CloneID	OBa218862
Clone ID Related Accession (Same CDS sequence)	XM_024990950.1
Accession Version	XM_024990950.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAGTCCTG TGAGAATGCC 
AACCCTTCTC TGGGCAGAAG AATGTCAGTT AATGGTGAGA AGCTGTTGAA GAGAGAAAAA 
CCAAGGGAGT TGATTTTTCC ATCTAATTAT GACCTCCTAC GACACCTGCA GTATGCAACC 
CATTTTCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTT 
CTTTATGGAG TGGGAAAAGA GCGTGACGAA GCCAGACATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC AACACAGAGA CAGGGGTTGG AGATGAAGGA 
CAGAAAGCCA GGAAGAGCAA ACAGGAAGCA TTTCCGACCC TGGAGACTGT GTTCACAAAA 
CTTCAACTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATTTC TAACAATGTG 
CTAGAACAAA TCACAAGCTT TGCATCAGGA ACATCATACC ATCTCCCATT GGCTCACCAC 
ATTCAGCTCA TCTTTGATCT CATGGAGCCA GCACTTAATA TTAATGGACT CATTGACTTC 
GCAATACAGC TCCTGAACGA ACTGAGTGTT GTGGAGGCAG AACTGCTCCT GAAGTCCTCC 
AGCCTGGCAG GAAGCTACAC GACAGGACTG TGTGTCTGCA TCGTGGCTGT TCTCAGGCGT 
TACCACAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGATTGTGT 
GGTGTGGTAA AGCACGTTGT GAACCCCTCA GAGTGCTCTT CCCCCGAAAG ATGCATCTTA 
GCCTACCTCT ACGACCTCTA CGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTGCCT GTTCAAAAGT AAAGCAAACC ATATATAACA ATGTGATTCC TGCAAATTCT 
AACTTGCGAT GGGATCCAGA CTTCATGATG GATTTTATTG AGAATCCCTC GGCCCGTAGC 
GTCAACTACT CAGTGCTGGG CAAGATCCTT AGTGACAATG CAGCCAATCG CTACAGCTTC 
GTCTGCAACA CTCTCATGAA TGTATGTATG GGTCATCAGG ATGCTGGAAG GATTAATGAC 
ATAGCCAATT TCTCCTCCGA GCTGACAGCC TGCTGCACTG TTCTCAGTTC AGAATGGCTG 
GGGGTCCTGA AGGCTCTTTG TTGTTCTTCA AATCATGTGT GGGGCTTTAA TGATGTTCTT 
TGCACTGTCG ATGTGAGTGA CCTTTCATTC CATGATTCAT TAGCTACTTT CATTGCTATT 
CTGATAGCAC GACAGTGTTT CTCCCTGGAG GACGTCGTGC AACACGTCGC ACTCCCCTCT 
CTCCTCGCCG CAGCTTGCGG AGATGCAGAT GCAGAGCCTG GGGCAAGAAT GACATGCCGT 
CTCCTGCTTC ATCTCTTTCG AGCTCCCCAG GCCTGCCTGT TACCTCAAGC AACTGGCAAA 
CCTTTCCCGG GAATAAGATC GTCTTGTGAC AGACACCTCT TAGCTGCTGC TCACAACAGC 
ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA AAAGCAATTA TGATGCTTGG AGATGCCAAA 
ATTGGCAATA ACAGTGTCAG CTCTTTAAAA AATGATGACT TCACCATGAG GGGTTTACGA 
CGTGATGGGA ATGCTGAGGA TATCTGGACT GCCTCACAAA ATTCAAAATC CTGTGGGAAA 
AGCATTTCCA TAGAAACGGC CAATTTAAGA GAATATGCTA GATATGTACT GAGGACCATC 
TGTCAACAGG AATGGGTAGG AGAACATTGT TTAAAAGAAC CTGAGAGATT ATGCACAGAC 
AAAGAACTTA TTCTGGACCC TGTGCTCTCA AATATGCAAG CACAGAAATT ACTGCAGCTG 
ATCTGTTATC CTCATGGCAT TAAGGAATGT ACGGAGGGGG ACAACCTCCA AAGACAGCAC 
ATTAAACGCA TTCTTCAGAA TCTTGAGCAG TGGACGCTGA GGCAGTCCTG GCTGGAGCTT 
CAGTTAATGA TCAAACAGTG CTTGAAGGAC CCTGGCTCTG GTTCGGTGGC TGAAATGAAC 
AACCTGCTGG ACAATATTGC CAAGGCAACA ATAGAGGTGT TCCAGCAGTC CGCTGACCTA 
AACAATAACT CTTCTAATGC TGGCATGGGC CTCTTCAACC CAAATGGAAT TGGAAGTACT 
GATACAAGTA GCACGAGACA GAATGGAATA AAGACATTTC TAAGTTCCTC TGAACGCAGG 
GGTGTGTGGC TGGTGGCCCC CCTAATTGCC AGGCTGCCGA CTTCTGTGCA GGGAAGAGTG 
TTGAAAGCAG CTGGGGAAGA GCTGGAGAAA GGACAGCACT TGGGTTCTTC TTCCAAAAAG 
GAAAGAGACA GACAAAAGCA GAAAAGTATG TCTCTTTTGA GTCAACAACC ATTCCTCTCA 
CTGGTCCTTA CCTGCCTTAA GGGACAAGAT GAACAACGAG AAGGCCTTCT AACATCTCTG 
CAGAATCAAG TTAACCAGAT TTTAAGTAAC TGGAGAGAAG AACGATACCA GGATGACATC 
AAAGCACGAC AGATGATGCA TGAGGCATTG CAACTCCGCC TGAATTTGGT GGGAGGAATG 
TTTGACACAG TGCAGAGGAG CACCCAGTGG ACAACAGACT GGGCGCTTCT CCTCCTTCAG 
ATCATTACTT CTGGAACCGT TGACATGCAC ACCAACAATG AATTATTCAC AACAGTTCTT 
GACATGCTGG GTGTTTTAAT CAATGGAACA TTGGCCTCTG ACCTGTCGAA TGCATCCCCA 
GGGGGATCTG AAGAGAACAA ACGTGCATAC ATGAACTTAG TCAAGAAACT GAAAAAAGAG 
CTGGGGGACA AGCGGTCAGA AAGTATTGAC AAAGTTCGAC AGCTGTTACC TTTGCCGAAA 
CAGACGTGTG ACATCATTAC CTGTGAACCT ATGGGCTCCT TGATTGACAC AAAGGGAAAC 
AAAATTGCTG GATTCGATTC TATAGATAAA AAACAGGGTC TCCAGGTCTC CACGAAGCAG 
AAGGTGTCCC CGTGGGATTT GTTTGAGGGT CAGAAGAACC CGGCTCCTCT GTCCTGGGCC 
TGGTTCGGGA CTGTCCGAGT GGACCGGAAG GTGATCAAGT ATGAGGAGCA ACATCACCTC 
CTCCTGTATC ACACGCACCC CACGCCCAAG CCCCGGAGTT ACTACCTCGA GCCACTGCCC 
CTGCCTCCCG AAGAGGAAGA GGAAGAGCCC ACATCTCCCA TCTCTCAGGA ACCTGAGAGG 
AAATCAGCTG AGCTGTCAGA TCAGGGAAAA ACCGCAACAG ATGAAGAGAA GAAGACAAAG 
GGAAGGAAGC GCAAGACAAA GTCCAGCTCA AGGGTTGATG AGTATCCGCA GAGCAGCATG 
TACCGAGTGC CTCCCAACTA CTCACCCATC TCCTCCCAGG TGATGCACCA CCCGCAGTCC 
GCCCTGTGGG GCTACAACCT CATGGGTCAG CCCCAGCAGC CCGGCTTCTT CCTTCAGAAC 
CAGTCTGTCA CTCCAGGTGG CTCCCGGTTG GACCCCGCTG GCTCCTTCGT CCCCACCAAC 
ACCAAGCAAG CTTTGTCCAA CATGCTCCAG CGGCGCTCGG GGGCCATGAT GCAGCCGCCC 
TCTCTGCATG CCATCACATC GCAGCAGCAG TTGATACAGA TGAAGCTTCT GCAGCAGCAG 
CAGCAACAGC GGCTTCTCAG GCAAGCCCAG ACGCGGCCTT TCCAACAGGG CCAGCCAGGG 
GACCAGGCTG CTCTCTTTGC TGCTCAGGCA CGGCCCTCCC CTCAGCTCCC CCAGTATCCA 
GGACTGCAGC AAGCACAGAC CATGCCCCAG GGCTACACAA TGTATGGAAC CCAGATGCCT 
TTGCAGCAGA CCCCTCAGCA GCAGGCTGGC AGCGTGGTCC TGTCTCCTAC CTATAATTCC 
AGAACCTATC CGGCCGCTCA CTCCAACCCA GCGCTCATGG AAAGACTCAG ACAGATGCAG 
CAGCCGCCGA GCGGCTATGT TCAGCAGCAG GCGTCACCAT ACCTGCATCC CCTGACCGGC 
TCCCAGAGGC TGAATCATCA GTCGCTTCAG CAGAGCCCTC TGGTGGGTGG AGGAATCGAT 
GCGGTGCTGA CTTCCGCACA TCCAAACCTT CCCTCAGTGC CCTTGTCTCA GGACCCGATG 
AGACCCAGAC AGCCACAGGT TCGACAGCAG CAGAGGCTTC TCCAGATGCA GCAGCCCCAG 
CAGCCCCCCC AGCCCCAGCA GCCTCCCCAG CCTCAGGGGT CCTCGCAGAC CCAGAGTCAG 
ACCCTTGGTC TCCAAACAAT GCAGCCCCAG CAGCCTCTGT TCCCCAGGCA AGGCTTGCAG 
CAGACCCAGC AGCAACAGCA GACGGCTGCC CTGGTGCGGC AGCTCCAGAA GCAGCTTTCC 
AGTAACCAGC CACAGCAAGG AGTGACTCCC TACGGGCATC CTTCACACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846718.1
CDS	414..6887
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_024990950.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990950.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAGTCCTG TGAGAATGCC 
AACCCTTCTC TGGGCAGAAG AATGTCAGTT AATGGTGAGA AGCTGTTGAA GAGAGAAAAA 
CCAAGGGAGT TGATTTTTCC ATCTAATTAT GACCTCCTAC GACACCTGCA GTATGCAACC 
CATTTTCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTT 
CTTTATGGAG TGGGAAAAGA GCGTGACGAA GCCAGACATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC AACACAGAGA CAGGGGTTGG AGATGAAGGA 
CAGAAAGCCA GGAAGAGCAA ACAGGAAGCA TTTCCGACCC TGGAGACTGT GTTCACAAAA 
CTTCAACTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATTTC TAACAATGTG 
CTAGAACAAA TCACAAGCTT TGCATCAGGA ACATCATACC ATCTCCCATT GGCTCACCAC 
ATTCAGCTCA TCTTTGATCT CATGGAGCCA GCACTTAATA TTAATGGACT CATTGACTTC 
GCAATACAGC TCCTGAACGA ACTGAGTGTT GTGGAGGCAG AACTGCTCCT GAAGTCCTCC 
AGCCTGGCAG GAAGCTACAC GACAGGACTG TGTGTCTGCA TCGTGGCTGT TCTCAGGCGT 
TACCACAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGATTGTGT 
GGTGTGGTAA AGCACGTTGT GAACCCCTCA GAGTGCTCTT CCCCCGAAAG ATGCATCTTA 
GCCTACCTCT ACGACCTCTA CGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTGCCT GTTCAAAAGT AAAGCAAACC ATATATAACA ATGTGATTCC TGCAAATTCT 
AACTTGCGAT GGGATCCAGA CTTCATGATG GATTTTATTG AGAATCCCTC GGCCCGTAGC 
GTCAACTACT CAGTGCTGGG CAAGATCCTT AGTGACAATG CAGCCAATCG CTACAGCTTC 
GTCTGCAACA CTCTCATGAA TGTATGTATG GGTCATCAGG ATGCTGGAAG GATTAATGAC 
ATAGCCAATT TCTCCTCCGA GCTGACAGCC TGCTGCACTG TTCTCAGTTC AGAATGGCTG 
GGGGTCCTGA AGGCTCTTTG TTGTTCTTCA AATCATGTGT GGGGCTTTAA TGATGTTCTT 
TGCACTGTCG ATGTGAGTGA CCTTTCATTC CATGATTCAT TAGCTACTTT CATTGCTATT 
CTGATAGCAC GACAGTGTTT CTCCCTGGAG GACGTCGTGC AACACGTCGC ACTCCCCTCT 
CTCCTCGCCG CAGCTTGCGG AGATGCAGAT GCAGAGCCTG GGGCAAGAAT GACATGCCGT 
CTCCTGCTTC ATCTCTTTCG AGCTCCCCAG GCCTGCCTGT TACCTCAAGC AACTGGCAAA 
CCTTTCCCGG GAATAAGATC GTCTTGTGAC AGACACCTCT TAGCTGCTGC TCACAACAGC 
ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA AAAGCAATTA TGATGCTTGG AGATGCCAAA 
ATTGGCAATA ACAGTGTCAG CTCTTTAAAA AATGATGACT TCACCATGAG GGGTTTACGA 
CGTGATGGGA ATGCTGAGGA TATCTGGACT GCCTCACAAA ATTCAAAATC CTGTGGGAAA 
AGCATTTCCA TAGAAACGGC CAATTTAAGA GAATATGCTA GATATGTACT GAGGACCATC 
TGTCAACAGG AATGGGTAGG AGAACATTGT TTAAAAGAAC CTGAGAGATT ATGCACAGAC 
AAAGAACTTA TTCTGGACCC TGTGCTCTCA AATATGCAAG CACAGAAATT ACTGCAGCTG 
ATCTGTTATC CTCATGGCAT TAAGGAATGT ACGGAGGGGG ACAACCTCCA AAGACAGCAC 
ATTAAACGCA TTCTTCAGAA TCTTGAGCAG TGGACGCTGA GGCAGTCCTG GCTGGAGCTT 
CAGTTAATGA TCAAACAGTG CTTGAAGGAC CCTGGCTCTG GTTCGGTGGC TGAAATGAAC 
AACCTGCTGG ACAATATTGC CAAGGCAACA ATAGAGGTGT TCCAGCAGTC CGCTGACCTA 
AACAATAACT CTTCTAATGC TGGCATGGGC CTCTTCAACC CAAATGGAAT TGGAAGTACT 
GATACAAGTA GCACGAGACA GAATGGAATA AAGACATTTC TAAGTTCCTC TGAACGCAGG 
GGTGTGTGGC TGGTGGCCCC CCTAATTGCC AGGCTGCCGA CTTCTGTGCA GGGAAGAGTG 
TTGAAAGCAG CTGGGGAAGA GCTGGAGAAA GGACAGCACT TGGGTTCTTC TTCCAAAAAG 
GAAAGAGACA GACAAAAGCA GAAAAGTATG TCTCTTTTGA GTCAACAACC ATTCCTCTCA 
CTGGTCCTTA CCTGCCTTAA GGGACAAGAT GAACAACGAG AAGGCCTTCT AACATCTCTG 
CAGAATCAAG TTAACCAGAT TTTAAGTAAC TGGAGAGAAG AACGATACCA GGATGACATC 
AAAGCACGAC AGATGATGCA TGAGGCATTG CAACTCCGCC TGAATTTGGT GGGAGGAATG 
TTTGACACAG TGCAGAGGAG CACCCAGTGG ACAACAGACT GGGCGCTTCT CCTCCTTCAG 
ATCATTACTT CTGGAACCGT TGACATGCAC ACCAACAATG AATTATTCAC AACAGTTCTT 
GACATGCTGG GTGTTTTAAT CAATGGAACA TTGGCCTCTG ACCTGTCGAA TGCATCCCCA 
GGGGGATCTG AAGAGAACAA ACGTGCATAC ATGAACTTAG TCAAGAAACT GAAAAAAGAG 
CTGGGGGACA AGCGGTCAGA AAGTATTGAC AAAGTTCGAC AGCTGTTACC TTTGCCGAAA 
CAGACGTGTG ACATCATTAC CTGTGAACCT ATGGGCTCCT TGATTGACAC AAAGGGAAAC 
AAAATTGCTG GATTCGATTC TATAGATAAA AAACAGGGTC TCCAGGTCTC CACGAAGCAG 
AAGGTGTCCC CGTGGGATTT GTTTGAGGGT CAGAAGAACC CGGCTCCTCT GTCCTGGGCC 
TGGTTCGGGA CTGTCCGAGT GGACCGGAAG GTGATCAAGT ATGAGGAGCA ACATCACCTC 
CTCCTGTATC ACACGCACCC CACGCCCAAG CCCCGGAGTT ACTACCTCGA GCCACTGCCC 
CTGCCTCCCG AAGAGGAAGA GGAAGAGCCC ACATCTCCCA TCTCTCAGGA ACCTGAGAGG 
AAATCAGCTG AGCTGTCAGA TCAGGGAAAA ACCGCAACAG ATGAAGAGAA GAAGACAAAG 
GGAAGGAAGC GCAAGACAAA GTCCAGCTCA AGGGTTGATG AGTATCCGCA GAGCAGCATG 
TACCGAGTGC CTCCCAACTA CTCACCCATC TCCTCCCAGG TGATGCACCA CCCGCAGTCC 
GCCCTGTGGG GCTACAACCT CATGGGTCAG CCCCAGCAGC CCGGCTTCTT CCTTCAGAAC 
CAGTCTGTCA CTCCAGGTGG CTCCCGGTTG GACCCCGCTG GCTCCTTCGT CCCCACCAAC 
ACCAAGCAAG CTTTGTCCAA CATGCTCCAG CGGCGCTCGG GGGCCATGAT GCAGCCGCCC 
TCTCTGCATG CCATCACATC GCAGCAGCAG TTGATACAGA TGAAGCTTCT GCAGCAGCAG 
CAGCAACAGC GGCTTCTCAG GCAAGCCCAG ACGCGGCCTT TCCAACAGGG CCAGCCAGGG 
GACCAGGCTG CTCTCTTTGC TGCTCAGGCA CGGCCCTCCC CTCAGCTCCC CCAGTATCCA 
GGACTGCAGC AAGCACAGAC CATGCCCCAG GGCTACACAA TGTATGGAAC CCAGATGCCT 
TTGCAGCAGA CCCCTCAGCA GCAGGCTGGC AGCGTGGTCC TGTCTCCTAC CTATAATTCC 
AGAACCTATC CGGCCGCTCA CTCCAACCCA GCGCTCATGG AAAGACTCAG ACAGATGCAG 
CAGCCGCCGA GCGGCTATGT TCAGCAGCAG GCGTCACCAT ACCTGCATCC CCTGACCGGC 
TCCCAGAGGC TGAATCATCA GTCGCTTCAG CAGAGCCCTC TGGTGGGTGG AGGAATCGAT 
GCGGTGCTGA CTTCCGCACA TCCAAACCTT CCCTCAGTGC CCTTGTCTCA GGACCCGATG 
AGACCCAGAC AGCCACAGGT TCGACAGCAG CAGAGGCTTC TCCAGATGCA GCAGCCCCAG 
CAGCCCCCCC AGCCCCAGCA GCCTCCCCAG CCTCAGGGGT CCTCGCAGAC CCAGAGTCAG 
ACCCTTGGTC TCCAAACAAT GCAGCCCCAG CAGCCTCTGT TCCCCAGGCA AGGCTTGCAG 
CAGACCCAGC AGCAACAGCA GACGGCTGCC CTGGTGCGGC AGCTCCAGAA GCAGCTTTCC 
AGTAACCAGC CACAGCAAGG AGTGACTCCC TACGGGCATC CTTCACACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_005201810.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201867.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X5, mRNA.

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CloneID	OBa218863
Clone ID Related Accession (Same CDS sequence)	XM_005201810.4 , NM_001206505.2
Accession Version	XM_005201810.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201810.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CCATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC GAAGTATTCA GTGCCAATGG TTCGAGCAAC ATGGCTGATC 
AAGATGACGT GTGCCTATTA CTCTGCTATT TCTGAAGCGA AAATTAAGAA ACGTCCGGCC 
ACTGATCCCA ATTTGGAGTG GACGCAAATA TCTACCAGGT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCCG ACTTCTACCA CCTGGCTTCC AGCGCGGGCG ATGGCCCTGT CCCTGTGCCA 
CCCGACGTGG AGCAGGCCAT GAGGCAGTGG GAGTACAACG AAAAGTTGGC ATTCCACATG 
TTCCAGGAAG GAATGCTGGA AAAGCATGAA TATTTGACAT GGATATTGGA TGTGTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTAT TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTCCAGTC GGCCTACCTG TCCCGTCGTC TCGCCTATTT CTGTGCCCGG 
CGTCTGGCGT TGCTGCTGAG CGACAGCCCC AGCCTCCTTG CCGCCCACTC GCCCCATGTG 
ATAATAGGCC CAAACAGCTC GAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGGC 
ATGAGCCCTG TGCAGCTGGC CTTCTCCGAC TTTCTTTCCT GTGCACAGCA CGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTTACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTACT CCACAAACGA CAATAAGAGC GCGAACCCGG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGCCT CCCCATGCCG GGAGGAAACA CGGCTTTCAA CCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCG ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AGGTATTGGA TCGACACTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGACAACCA AGAGGTTGCC 
CCCAACGATG AAGCTGTGGT GACGCTGCTG TGTGAATGGG CTGTGAGCTG TAAGCGGTCA 
GGCAAGCACA GGGCGATGGC TGTGGCGAAA CTCCTGGAGA AGAGACAAGC GGAAATCGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCCATTTC CTCAGCCTCC 
CTCGCTGGAT CTAGTTTACC TGTTTTCCAG AATGTTCTTC TAAGGTTTTT AGATACACAG 
GCCCCCTCGT TGTCGGATCC AAATAGCGAA TGTGAGAAGG TGGAGTTTGT CAACCTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGGCATGAC GTCTTCTCCC ACGATGCCTA CATGTGCACC 
CTCATCTCTC GGGGAGACCT GTCTGTCACC GCCTCCACGC GACCGCGGTC GCCAGCAGGA 
GAGAATACGG ATGAGCATTA TCCAAAGGAC CACGACATGA AAATGGAGAT TTTTTCTCCC 
ATGCCTGGAG AGTCCTGTGA GAATGCCAAC CCTTCTCTGG GCAGAAGAAT GTCAGTTAAT 
GGTGAGAAGC TGTTGAAGAG AGAAAAACCA AGGGAGTTGA TTTTTCCATC TAATTATGAC 
CTCCTACGAC ACCTGCAGTA TGCAACCCAT TTTCCCATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTTCTT TATGGAGTGG GAAAAGAGCG TGACGAAGCC 
AGACATCAGC TGAAGAAGAT TACCAAAGAT ATTTTGAAAA TCCTAAATAA GAAGAGCAAC 
ACAGAGACAG GGGTTGGAGA TGAAGGACAG AAAGCCAGGA AGAGCAAACA GGAAGCATTT 
CCGACCCTGG AGACTGTGTT CACAAAACTT CAACTCCTTT CATATTTTGA TCAGCATCAA 
GTGACATCTC AGATTTCTAA CAATGTGCTA GAACAAATCA CAAGCTTTGC ATCAGGAACA 
TCATACCATC TCCCATTGGC TCACCACATT CAGCTCATCT TTGATCTCAT GGAGCCAGCA 
CTTAATATTA ATGGACTCAT TGACTTCGCA ATACAGCTCC TGAACGAACT GAGTGTTGTG 
GAGGCAGAAC TGCTCCTGAA GTCCTCCAGC CTGGCAGGAA GCTACACGAC AGGACTGTGT 
GTCTGCATCG TGGCTGTTCT CAGGCGTTAC CACAGTTGTC TGATCCTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG ATTGTGTGGT GTGGTAAAGC ACGTTGTGAA CCCCTCAGAG 
TGCTCTTCCC CCGAAAGATG CATCTTAGCC TACCTCTACG ACCTCTACGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT AAAGCAAACC 
ATATATAACA ATGTGATTCC TGCAAATTCT AACTTGCGAT GGGATCCAGA CTTCATGATG 
GATTTTATTG AGAATCCCTC GGCCCGTAGC GTCAACTACT CAGTGCTGGG CAAGATCCTT 
AGTGACAATG CAGCCAATCG CTACAGCTTC GTCTGCAACA CTCTCATGAA TGTATGTATG 
GGTCATCAGG ATGCTGGAAG GATTAATGAC ATAGCCAATT TCTCCTCCGA GCTGACAGCC 
TGCTGCACTG TTCTCAGTTC AGAATGGCTG GGGGTCCTGA AGGCTCTTTG TTGTTCTTCA 
AATCATGTGT GGGGCTTTAA TGATGTTCTT TGCACTGTCG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT CTCCCTGGAG 
GACGTCGTGC AACACGTCGC ACTCCCCTCT CTCCTCGCCG CAGCTTGCGG AGATGCAGAT 
GCAGAGCCTG GGGCAAGAAT GACATGCCGT CTCCTGCTTC ATCTCTTTCG AGCTCCCCAG 
GCCTGCCTGT TACCTCAAGC AACTGGCAAA CCTTTCCCGG GAATAAGATC GTCTTGTGAC 
AGACACCTCT TAGCTGCTGC TCACAACAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAGTGTCAG CTCTTTAAAA 
AATGATGACT TCACCATGAG GGGTTTACGA CGTGATGGGA ATGCTGAGGA TATCTGGACT 
GCCTCACAAA ATTCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACGGC CAATTTAAGA 
GAATATGCTA GATATGTACT GAGGACCATC TGTCAACAGG AATGGGTAGG AGAACATTGT 
TTAAAAGAAC CTGAGAGATT ATGCACAGAC AAAGAACTTA TTCTGGACCC TGTGCTCTCA 
AATATGCAAG CACAGAAATT ACTGCAGCTG ATCTGTTATC CTCATGGCAT TAAGGAATGT 
ACGGAGGGGG ACAACCTCCA AAGACAGCAC ATTAAACGCA TTCTTCAGAA TCTTGAGCAG 
TGGACGCTGA GGCAGTCCTG GCTGGAGCTT CAGTTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCGGTGGC TGAAATGAAC AACCTGCTGG ACAATATTGC CAAGGCAACA 
ATAGAGGTGT TCCAGCAGTC CGCTGACCTA AACAATAACT CTTCTAATGC TGGCATGGGC 
CTCTTCAACC CAAATGGAAT TGGAAGTACT GATACAAGTA GCACGAGACA GAATGGAATA 
AAGACATTTC TAAGTTCCTC TGAACGCAGG GGTGTGTGGC TGGTGGCCCC CCTAATTGCC 
AGGCTGCCGA CTTCTGTGCA GGGAAGAGTG TTGAAAGCAG CTGGGGAAGA GCTGGAGAAA 
GGACAGCACT TGGGTTCTTC TTCCAAAAAG GAAAGAGACA GACAAAAGCA GAAAAGTATG 
TCTCTTTTGA GTCAACAACC ATTCCTCTCA CTGGTCCTTA CCTGCCTTAA GGGACAAGAT 
GAACAACGAG AAGGCCTTCT AACATCTCTG CAGAATCAAG TTAACCAGAT TTTAAGTAAC 
TGGAGAGAAG AACGATACCA GGATGACATC AAAGCACGAC AGATGATGCA TGAGGCATTG 
CAACTCCGCC TGAATTTGGT GGGAGGAATG TTTGACACAG TGCAGAGGAG CACCCAGTGG 
ACAACAGACT GGGCGCTTCT CCTCCTTCAG ATCATTACTT CTGGAACCGT TGACATGCAC 
ACCAACAATG AATTATTCAC AACAGTTCTT GACATGCTGG GTGTTTTAAT CAATGGAACA 
TTGGCCTCTG ACCTGTCGAA TGCATCCCCA GGGGGATCTG AAGAGAACAA ACGTGCATAC 
ATGAACTTAG TCAAGAAACT GAAAAAAGAG CTGGGGGACA AGCGGTCAGA AAGTATTGAC 
AAAGTTCGAC AGCTGTTACC TTTGCCGAAA CAGACGTGTG ACATCATTAC CTGTGAACCT 
ATGGGCTCCT TGATTGACAC AAAGGGAAAC AAAATTGCTG GATTCGATTC TATAGATAAA 
AAACAGGGTC TCCAGGTCTC CACGAAGCAG AAGGTGTCCC CGTGGGATTT GTTTGAGGGT 
CAGAAGAACC CGGCTCCTCT GTCCTGGGCC TGGTTCGGGA CTGTCCGAGT GGACCGGAAG 
GTGATCAAGT ATGAGGAGCA ACATCACCTC CTCCTGTATC ACACGCACCC CACGCCCAAG 
CCCCGGAGTT ACTACCTCGA GCCACTGCCC CTGCCTCCCG AAGAGGAAGA GGAAGAGCCC 
ACATCTCCCA TCTCTCAGGA ACCTGAGAGG AAATCAGCTG AGCTGTCAGA TCAGGGAAAA 
ACCGCAACAG ATGAAGAGAA GAAGACAAAG GGAAGGAAGC GCAAGACAAA GTCCAGCTCA 
AGGGTTGATG AGTATCCGCA GAGCAGCATG TACCGAGTGC CTCCCAACTA CTCACCCATC 
TCCTCCCAGG TGATGCACCA CCCGCAGTCC GCCCTGTGGG GCTACAACCT CATGGGTCAG 
CCCCAGCAGC CCGGCTTCTT CCTTCAGAAC CAGTCTGTCA CTCCAGGTGG CTCCCGGTTG 
GACCCCGCTG GCTCCTTCGT CCCCACCAAC ACCAAGCAAG CTTTGTCCAA CATGCTCCAG 
CGGCGCTCGG GGGCCATGAT GCAGCCGCCC TCTCTGCATG CCATCACATC GCAGCAGCAG 
TTGATACAGA TGAAGCTTCT GCAGCAGCAG CAGCAACAGC GGCTTCTCAG GCAAGCCCAG 
ACGCGGCCTT TCCAACAGGG CCAGCCAGGG GACCAGGCTG CTCTCTTTGC TGCTCAGGCA 
CGGCCCTCCC CTCAGCTCCC CCAGTATCCA GGACTGCAGC AAGCACAGAC CATGCCCCAG 
GGCTACACAA TGTATGGAAC CCAGATGCCT TTGCAGCAGA CCCCTCAGCA GCAGGCTGGC 
AGCGTGGTCC TGTCTCCTAC CTATAATTCC AGAACCTATC CGGCCGCTCA CTCCAACCCA 
GCGCTCATGG AAAGACTCAG ACAGATGCAG CAGCCGCCGA GCGGCTATGT TCAGCAGCAG 
GCGTCACCAT ACCTGCATCC CCTGACCGGC TCCCAGAGGC TGAATCATCA GTCGCTTCAG 
CAGAGCCCTC TGGTGGGTGG AGGAATCGAT GCGGTGCTGA CTTCCGCACA TCCAAACCTT 
CCCTCAGTGC CCTTGTCTCA GGACCCGATG AGACCCAGAC AGCCACAGGT TCGACAGCAG 
CAGAGGCTTC TCCAGATGCA GCAGCCCCAG CAGCCCCCCC AGCCCCAGCA GCCTCCCCAG 
CCTCAGGGGT CCTCGCAGAC CCAGAGTCAG ACCCTTGGTC TCCAAACAAT GCAGCCCCAG 
CAGCCTCTGT TCCCCAGGCA AGGCTTGCAG CAGACCCAGC AGCAACAGCA GACGGCTGCC 
CTGGTGCGGC AGCTCCAGAA GCAGCTTTCC AGTAACCAGC CACAGCAAGG AGTGACTCCC 
TACGGGCATC CTTCACACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201867.1
CDS	414..6857
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_005201810.4
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201810.4

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CCATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC GAAGTATTCA GTGCCAATGG TTCGAGCAAC ATGGCTGATC 
AAGATGACGT GTGCCTATTA CTCTGCTATT TCTGAAGCGA AAATTAAGAA ACGTCCGGCC 
ACTGATCCCA ATTTGGAGTG GACGCAAATA TCTACCAGGT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCCG ACTTCTACCA CCTGGCTTCC AGCGCGGGCG ATGGCCCTGT CCCTGTGCCA 
CCCGACGTGG AGCAGGCCAT GAGGCAGTGG GAGTACAACG AAAAGTTGGC ATTCCACATG 
TTCCAGGAAG GAATGCTGGA AAAGCATGAA TATTTGACAT GGATATTGGA TGTGTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTAT TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTCCAGTC GGCCTACCTG TCCCGTCGTC TCGCCTATTT CTGTGCCCGG 
CGTCTGGCGT TGCTGCTGAG CGACAGCCCC AGCCTCCTTG CCGCCCACTC GCCCCATGTG 
ATAATAGGCC CAAACAGCTC GAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGGC 
ATGAGCCCTG TGCAGCTGGC CTTCTCCGAC TTTCTTTCCT GTGCACAGCA CGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTTACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTACT CCACAAACGA CAATAAGAGC GCGAACCCGG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGCCT CCCCATGCCG GGAGGAAACA CGGCTTTCAA CCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCG ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AGGTATTGGA TCGACACTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGACAACCA AGAGGTTGCC 
CCCAACGATG AAGCTGTGGT GACGCTGCTG TGTGAATGGG CTGTGAGCTG TAAGCGGTCA 
GGCAAGCACA GGGCGATGGC TGTGGCGAAA CTCCTGGAGA AGAGACAAGC GGAAATCGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCCATTTC CTCAGCCTCC 
CTCGCTGGAT CTAGTTTACC TGTTTTCCAG AATGTTCTTC TAAGGTTTTT AGATACACAG 
GCCCCCTCGT TGTCGGATCC AAATAGCGAA TGTGAGAAGG TGGAGTTTGT CAACCTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGGCATGAC GTCTTCTCCC ACGATGCCTA CATGTGCACC 
CTCATCTCTC GGGGAGACCT GTCTGTCACC GCCTCCACGC GACCGCGGTC GCCAGCAGGA 
GAGAATACGG ATGAGCATTA TCCAAAGGAC CACGACATGA AAATGGAGAT TTTTTCTCCC 
ATGCCTGGAG AGTCCTGTGA GAATGCCAAC CCTTCTCTGG GCAGAAGAAT GTCAGTTAAT 
GGTGAGAAGC TGTTGAAGAG AGAAAAACCA AGGGAGTTGA TTTTTCCATC TAATTATGAC 
CTCCTACGAC ACCTGCAGTA TGCAACCCAT TTTCCCATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTTCTT TATGGAGTGG GAAAAGAGCG TGACGAAGCC 
AGACATCAGC TGAAGAAGAT TACCAAAGAT ATTTTGAAAA TCCTAAATAA GAAGAGCAAC 
ACAGAGACAG GGGTTGGAGA TGAAGGACAG AAAGCCAGGA AGAGCAAACA GGAAGCATTT 
CCGACCCTGG AGACTGTGTT CACAAAACTT CAACTCCTTT CATATTTTGA TCAGCATCAA 
GTGACATCTC AGATTTCTAA CAATGTGCTA GAACAAATCA CAAGCTTTGC ATCAGGAACA 
TCATACCATC TCCCATTGGC TCACCACATT CAGCTCATCT TTGATCTCAT GGAGCCAGCA 
CTTAATATTA ATGGACTCAT TGACTTCGCA ATACAGCTCC TGAACGAACT GAGTGTTGTG 
GAGGCAGAAC TGCTCCTGAA GTCCTCCAGC CTGGCAGGAA GCTACACGAC AGGACTGTGT 
GTCTGCATCG TGGCTGTTCT CAGGCGTTAC CACAGTTGTC TGATCCTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG ATTGTGTGGT GTGGTAAAGC ACGTTGTGAA CCCCTCAGAG 
TGCTCTTCCC CCGAAAGATG CATCTTAGCC TACCTCTACG ACCTCTACGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT AAAGCAAACC 
ATATATAACA ATGTGATTCC TGCAAATTCT AACTTGCGAT GGGATCCAGA CTTCATGATG 
GATTTTATTG AGAATCCCTC GGCCCGTAGC GTCAACTACT CAGTGCTGGG CAAGATCCTT 
AGTGACAATG CAGCCAATCG CTACAGCTTC GTCTGCAACA CTCTCATGAA TGTATGTATG 
GGTCATCAGG ATGCTGGAAG GATTAATGAC ATAGCCAATT TCTCCTCCGA GCTGACAGCC 
TGCTGCACTG TTCTCAGTTC AGAATGGCTG GGGGTCCTGA AGGCTCTTTG TTGTTCTTCA 
AATCATGTGT GGGGCTTTAA TGATGTTCTT TGCACTGTCG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT CTCCCTGGAG 
GACGTCGTGC AACACGTCGC ACTCCCCTCT CTCCTCGCCG CAGCTTGCGG AGATGCAGAT 
GCAGAGCCTG GGGCAAGAAT GACATGCCGT CTCCTGCTTC ATCTCTTTCG AGCTCCCCAG 
GCCTGCCTGT TACCTCAAGC AACTGGCAAA CCTTTCCCGG GAATAAGATC GTCTTGTGAC 
AGACACCTCT TAGCTGCTGC TCACAACAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAGTGTCAG CTCTTTAAAA 
AATGATGACT TCACCATGAG GGGTTTACGA CGTGATGGGA ATGCTGAGGA TATCTGGACT 
GCCTCACAAA ATTCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACGGC CAATTTAAGA 
GAATATGCTA GATATGTACT GAGGACCATC TGTCAACAGG AATGGGTAGG AGAACATTGT 
TTAAAAGAAC CTGAGAGATT ATGCACAGAC AAAGAACTTA TTCTGGACCC TGTGCTCTCA 
AATATGCAAG CACAGAAATT ACTGCAGCTG ATCTGTTATC CTCATGGCAT TAAGGAATGT 
ACGGAGGGGG ACAACCTCCA AAGACAGCAC ATTAAACGCA TTCTTCAGAA TCTTGAGCAG 
TGGACGCTGA GGCAGTCCTG GCTGGAGCTT CAGTTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCGGTGGC TGAAATGAAC AACCTGCTGG ACAATATTGC CAAGGCAACA 
ATAGAGGTGT TCCAGCAGTC CGCTGACCTA AACAATAACT CTTCTAATGC TGGCATGGGC 
CTCTTCAACC CAAATGGAAT TGGAAGTACT GATACAAGTA GCACGAGACA GAATGGAATA 
AAGACATTTC TAAGTTCCTC TGAACGCAGG GGTGTGTGGC TGGTGGCCCC CCTAATTGCC 
AGGCTGCCGA CTTCTGTGCA GGGAAGAGTG TTGAAAGCAG CTGGGGAAGA GCTGGAGAAA 
GGACAGCACT TGGGTTCTTC TTCCAAAAAG GAAAGAGACA GACAAAAGCA GAAAAGTATG 
TCTCTTTTGA GTCAACAACC ATTCCTCTCA CTGGTCCTTA CCTGCCTTAA GGGACAAGAT 
GAACAACGAG AAGGCCTTCT AACATCTCTG CAGAATCAAG TTAACCAGAT TTTAAGTAAC 
TGGAGAGAAG AACGATACCA GGATGACATC AAAGCACGAC AGATGATGCA TGAGGCATTG 
CAACTCCGCC TGAATTTGGT GGGAGGAATG TTTGACACAG TGCAGAGGAG CACCCAGTGG 
ACAACAGACT GGGCGCTTCT CCTCCTTCAG ATCATTACTT CTGGAACCGT TGACATGCAC 
ACCAACAATG AATTATTCAC AACAGTTCTT GACATGCTGG GTGTTTTAAT CAATGGAACA 
TTGGCCTCTG ACCTGTCGAA TGCATCCCCA GGGGGATCTG AAGAGAACAA ACGTGCATAC 
ATGAACTTAG TCAAGAAACT GAAAAAAGAG CTGGGGGACA AGCGGTCAGA AAGTATTGAC 
AAAGTTCGAC AGCTGTTACC TTTGCCGAAA CAGACGTGTG ACATCATTAC CTGTGAACCT 
ATGGGCTCCT TGATTGACAC AAAGGGAAAC AAAATTGCTG GATTCGATTC TATAGATAAA 
AAACAGGGTC TCCAGGTCTC CACGAAGCAG AAGGTGTCCC CGTGGGATTT GTTTGAGGGT 
CAGAAGAACC CGGCTCCTCT GTCCTGGGCC TGGTTCGGGA CTGTCCGAGT GGACCGGAAG 
GTGATCAAGT ATGAGGAGCA ACATCACCTC CTCCTGTATC ACACGCACCC CACGCCCAAG 
CCCCGGAGTT ACTACCTCGA GCCACTGCCC CTGCCTCCCG AAGAGGAAGA GGAAGAGCCC 
ACATCTCCCA TCTCTCAGGA ACCTGAGAGG AAATCAGCTG AGCTGTCAGA TCAGGGAAAA 
ACCGCAACAG ATGAAGAGAA GAAGACAAAG GGAAGGAAGC GCAAGACAAA GTCCAGCTCA 
AGGGTTGATG AGTATCCGCA GAGCAGCATG TACCGAGTGC CTCCCAACTA CTCACCCATC 
TCCTCCCAGG TGATGCACCA CCCGCAGTCC GCCCTGTGGG GCTACAACCT CATGGGTCAG 
CCCCAGCAGC CCGGCTTCTT CCTTCAGAAC CAGTCTGTCA CTCCAGGTGG CTCCCGGTTG 
GACCCCGCTG GCTCCTTCGT CCCCACCAAC ACCAAGCAAG CTTTGTCCAA CATGCTCCAG 
CGGCGCTCGG GGGCCATGAT GCAGCCGCCC TCTCTGCATG CCATCACATC GCAGCAGCAG 
TTGATACAGA TGAAGCTTCT GCAGCAGCAG CAGCAACAGC GGCTTCTCAG GCAAGCCCAG 
ACGCGGCCTT TCCAACAGGG CCAGCCAGGG GACCAGGCTG CTCTCTTTGC TGCTCAGGCA 
CGGCCCTCCC CTCAGCTCCC CCAGTATCCA GGACTGCAGC AAGCACAGAC CATGCCCCAG 
GGCTACACAA TGTATGGAAC CCAGATGCCT TTGCAGCAGA CCCCTCAGCA GCAGGCTGGC 
AGCGTGGTCC TGTCTCCTAC CTATAATTCC AGAACCTATC CGGCCGCTCA CTCCAACCCA 
GCGCTCATGG AAAGACTCAG ACAGATGCAG CAGCCGCCGA GCGGCTATGT TCAGCAGCAG 
GCGTCACCAT ACCTGCATCC CCTGACCGGC TCCCAGAGGC TGAATCATCA GTCGCTTCAG 
CAGAGCCCTC TGGTGGGTGG AGGAATCGAT GCGGTGCTGA CTTCCGCACA TCCAAACCTT 
CCCTCAGTGC CCTTGTCTCA GGACCCGATG AGACCCAGAC AGCCACAGGT TCGACAGCAG 
CAGAGGCTTC TCCAGATGCA GCAGCCCCAG CAGCCCCCCC AGCCCCAGCA GCCTCCCCAG 
CCTCAGGGGT CCTCGCAGAC CCAGAGTCAG ACCCTTGGTC TCCAAACAAT GCAGCCCCAG 
CAGCCTCTGT TCCCCAGGCA AGGCTTGCAG CAGACCCAGC AGCAACAGCA GACGGCTGCC 
CTGGTGCGGC AGCTCCAGAA GCAGCTTTCC AGTAACCAGC CACAGCAAGG AGTGACTCCC 
TACGGGCATC CTTCACACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_024990954.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846722.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X6, mRNA.

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CloneID	OBa218864
Clone ID Related Accession (Same CDS sequence)	XM_024990954.1
Accession Version	XM_024990954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAGTCCTG TGAGAATGCC 
AACCCTTCTC TGGGCAGAAG AATGTCAGTT AATGGTGAGA AGCTGTTGAA GAGAGAAAAA 
CCAAGGGAGT TGATTTTTCC ATCTAATTAT GACCTCCTAC GACACCTGCA GTATGCAACC 
CATTTTCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTT 
CTTTATGGAG TGGGAAAAGA GCGTGACGAA GCCAGACATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC AACACAGAGA CAGGGGTTGG AGATGAAGGA 
CAGAAAGCCA GGAAGAGCAA ACAGGAAGCA TTTCCGACCC TGGAGACTGT GTTCACAAAA 
CTTCAACTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATTTC TAACAATGTG 
CTAGAACAAA TCACAAGCTT TGCATCAGGA ACATCATACC ATCTCCCATT GGCTCACCAC 
ATTCAGCTCA TCTTTGATCT CATGGAGCCA GCACTTAATA TTAATGGACT CATTGACTTC 
GCAATACAGC TCCTGAACGA ACTGAGTGTT GTGGAGGCAG AACTGCTCCT GAAGTCCTCC 
AGCCTGGCAG GAAGCTACAC GACAGGACTG TGTGTCTGCA TCGTGGCTGT TCTCAGGCGT 
TACCACAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGATTGTGT 
GGTGTGGTAA AGCACGTTGT GAACCCCTCA GAGTGCTCTT CCCCCGAAAG ATGCATCTTA 
GCCTACCTCT ACGACCTCTA CGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTAGTG CCTGTTCAAA AGTAAAGCAA ACCATATATA ACAATGTGAT TCCTGCAAAT 
TCTAACTTGC GATGGGATCC AGACTTCATG ATGGATTTTA TTGAGAATCC CTCGGCCCGT 
AGCGTCAACT ACTCAGTGCT GGGCAAGATC CTTAGTGACA ATGCAGCCAA TCGCTACAGC 
TTCGTCTGCA ACACTCTCAT GAATGTATGT ATGGGTCATC AGGATGCTGG AAGGATTAAT 
GACATAGCCA ATTTCTCCTC CGAGCTGACA GCCTGCTGCA CTGTTCTCAG TTCAGAATGG 
CTGGGGGTCC TGAAGGCTCT TTGTTGTTCT TCAAATCATG TGTGGGGCTT TAATGATGTT 
CTTTGCACTG TCGATGTGAG TGACCTTTCA TTCCATGATT CATTAGCTAC TTTCATTGCT 
ATTCTGATAG CACGACAGTG TTTCTCCCTG GAGGACGTCG TGCAACACGT CGCACTCCCC 
TCTCTCCTCG CCGCAGGCAA ACCTTTCCCG GGAATAAGAT CGTCTTGTGA CAGACACCTC 
TTAGCTGCTG CTCACAACAG CATTGAAGTG GGAGCCGTGT TTGCTGTCTT AAAAGCAATT 
ATGATGCTTG GAGATGCCAA AATTGGCAAT AACAGTGTCA GCTCTTTAAA AAATGATGAC 
TTCACCATGA GGGGTTTACG ACGTGATGGG AATGCTGAGG ATATCTGGAC TGCCTCACAA 
AATTCAAAAT CCTGTGGGAA AAGCATTTCC ATAGAAACGG CCAATTTAAG AGAATATGCT 
AGATATGTAC TGAGGACCAT CTGTCAACAG GAATGGGTAG GAGAACATTG TTTAAAAGAA 
CCTGAGAGAT TATGCACAGA CAAAGAACTT ATTCTGGACC CTGTGCTCTC AAATATGCAA 
GCACAGAAAT TACTGCAGCT GATCTGTTAT CCTCATGGCA TTAAGGAATG TACGGAGGGG 
GACAACCTCC AAAGACAGCA CATTAAACGC ATTCTTCAGA ATCTTGAGCA GTGGACGCTG 
AGGCAGTCCT GGCTGGAGCT TCAGTTAATG ATCAAACAGT GCTTGAAGGA CCCTGGCTCT 
GGTTCGGTGG CTGAAATGAA CAACCTGCTG GACAATATTG CCAAGGCAAC AATAGAGGTG 
TTCCAGCAGT CCGCTGACCT AAACAATAAC TCTTCTAATG CTGGCATGGG CCTCTTCAAC 
CCAAATGGAA TTGGAAGTAC TGATACAAGT AGCACGAGAC AGAATGGAAT AAAGACATTT 
CTAAGTTCCT CTGAACGCAG GGGTGTGTGG CTGGTGGCCC CCCTAATTGC CAGGCTGCCG 
ACTTCTGTGC AGGGAAGAGT GTTGAAAGCA GCTGGGGAAG AGCTGGAGAA AGGACAGCAC 
TTGGGTTCTT CTTCCAAAAA GGAAAGAGAC AGACAAAAGC AGAAAAGTAT GTCTCTTTTG 
AGTCAACAAC CATTCCTCTC ACTGGTCCTT ACCTGCCTTA AGGGACAAGA TGAACAACGA 
GAAGGCCTTC TAACATCTCT GCAGAATCAA GTTAACCAGA TTTTAAGTAA CTGGAGAGAA 
GAACGATACC AGGATGACAT CAAAGCACGA CAGATGATGC ATGAGGCATT GCAACTCCGC 
CTGAATTTGG TGGGAGGAAT GTTTGACACA GTGCAGAGGA GCACCCAGTG GACAACAGAC 
TGGGCGCTTC TCCTCCTTCA GATCATTACT TCTGGAACCG TTGACATGCA CACCAACAAT 
GAATTATTCA CAACAGTTCT TGACATGCTG GGTGTTTTAA TCAATGGAAC ATTGGCCTCT 
GACCTGTCGA ATGCATCCCC AGGGGGATCT GAAGAGAACA AACGTGCATA CATGAACTTA 
GTCAAGAAAC TGAAAAAAGA GCTGGGGGAC AAGCGGTCAG AAAGTATTGA CAAAGTTCGA 
CAGCTGTTAC CTTTGCCGAA ACAGACGTGT GACATCATTA CCTGTGAACC TATGGGCTCC 
TTGATTGACA CAAAGGGAAA CAAAATTGCT GGATTCGATT CTATAGATAA AAAACAGGGT 
CTCCAGGTCT CCACGAAGCA GAAGGTGTCC CCGTGGGATT TGTTTGAGGG TCAGAAGAAC 
CCGGCTCCTC TGTCCTGGGC CTGGTTCGGG ACTGTCCGAG TGGACCGGAA GGTGATCAAG 
TATGAGGAGC AACATCACCT CCTCCTGTAT CACACGCACC CCACGCCCAA GCCCCGGAGT 
TACTACCTCG AGCCACTGCC CCTGCCTCCC GAAGAGGAAG AGGAAGAGCC CACATCTCCC 
ATCTCTCAGG AACCTGAGAG GAAATCAGCT GAGCTGTCAG ATCAGGGAAA AACCGCAACA 
GATGAAGAGA AGAAGACAAA GGGAAGGAAG CGCAAGACAA AGTCCAGCTC AAGGGTTGAT 
GAGTATCCGC AGAGCAGCAT GTACCGAGTG CCTCCCAACT ACTCACCCAT CTCCTCCCAG 
GTGATGCACC ACCCGCAGTC CGCCCTGTGG GGCTACAACC TCATGGGTCA GCCCCAGCAG 
CCCGGCTTCT TCCTTCAGAA CCAGTCTGTC ACTCCAGGTG GCTCCCGGTT GGACCCCGCT 
GGCTCCTTCG TCCCCACCAA CACCAAGCAA GCTTTGTCCA ACATGCTCCA GCGGCGCTCG 
GGGGCCATGA TGCAGCCGCC CTCTCTGCAT GCCATCACAT CGCAGCAGCA GTTGATACAG 
ATGAAGCTTC TGCAGCAGCA GCAGCAACAG CGGCTTCTCA GGCAAGCCCA GACGCGGCCT 
TTCCAACAGG GCCAGCCAGG GGACCAGGCT GCTCTCTTTG CTGCTCAGGC ACGGCCCTCC 
CCTCAGCTCC CCCAGTATCC AGGACTGCAG CAAGCACAGA CCATGCCCCA GGGCTACACA 
ATGTATGGAA CCCAGATGCC TTTGCAGCAG ACCCCTCAGC AGCAGGCTGG CAGCGTGGTC 
CTGTCTCCTA CCTATAATTC CAGAACCTAT CCGGCCGCTC ACTCCAACCC AGCGCTCATG 
GAAAGACTCA GACAGATGCA GCAGCCGCCG AGCGGCTATG TTCAGCAGCA GGCGTCACCA 
TACCTGCATC CCCTGACCGG CTCCCAGAGG CTGAATCATC AGTCGCTTCA GCAGAGCCCT 
CTGGTGGGTG GAGGAATCGA TGCGGTGCTG ACTTCCGCAC ATCCAAACCT TCCCTCAGTG 
CCCTTGTCTC AGGACCCGAT GAGACCCAGA CAGCCACAGG TTCGACAGCA GCAGAGGCTT 
CTCCAGATGC AGCAGCCCCA GCAGCCCCCC CAGCCCCAGC AGCCTCCCCA GCCTCAGGGG 
TCCTCGCAGA CCCAGAGTCA GACCCTTGGT CTCCAAACAA TGCAGCCCCA GCAGCCTCTG 
TTCCCCAGGC AAGGCTTGCA GCAGACCCAG CAGCAACAGC AGACGGCTGC CCTGGTGCGG 
CAGCTCCAGA AGCAGCTTTC CAGTAACCAG CCACAGCAAG GAGTGACTCC CTACGGGCAT 
CCTTCACACT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846722.1
CDS	414..6788
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_024990954.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990954.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAGTCCTG TGAGAATGCC 
AACCCTTCTC TGGGCAGAAG AATGTCAGTT AATGGTGAGA AGCTGTTGAA GAGAGAAAAA 
CCAAGGGAGT TGATTTTTCC ATCTAATTAT GACCTCCTAC GACACCTGCA GTATGCAACC 
CATTTTCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTT 
CTTTATGGAG TGGGAAAAGA GCGTGACGAA GCCAGACATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC AACACAGAGA CAGGGGTTGG AGATGAAGGA 
CAGAAAGCCA GGAAGAGCAA ACAGGAAGCA TTTCCGACCC TGGAGACTGT GTTCACAAAA 
CTTCAACTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATTTC TAACAATGTG 
CTAGAACAAA TCACAAGCTT TGCATCAGGA ACATCATACC ATCTCCCATT GGCTCACCAC 
ATTCAGCTCA TCTTTGATCT CATGGAGCCA GCACTTAATA TTAATGGACT CATTGACTTC 
GCAATACAGC TCCTGAACGA ACTGAGTGTT GTGGAGGCAG AACTGCTCCT GAAGTCCTCC 
AGCCTGGCAG GAAGCTACAC GACAGGACTG TGTGTCTGCA TCGTGGCTGT TCTCAGGCGT 
TACCACAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGATTGTGT 
GGTGTGGTAA AGCACGTTGT GAACCCCTCA GAGTGCTCTT CCCCCGAAAG ATGCATCTTA 
GCCTACCTCT ACGACCTCTA CGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTAGTG CCTGTTCAAA AGTAAAGCAA ACCATATATA ACAATGTGAT TCCTGCAAAT 
TCTAACTTGC GATGGGATCC AGACTTCATG ATGGATTTTA TTGAGAATCC CTCGGCCCGT 
AGCGTCAACT ACTCAGTGCT GGGCAAGATC CTTAGTGACA ATGCAGCCAA TCGCTACAGC 
TTCGTCTGCA ACACTCTCAT GAATGTATGT ATGGGTCATC AGGATGCTGG AAGGATTAAT 
GACATAGCCA ATTTCTCCTC CGAGCTGACA GCCTGCTGCA CTGTTCTCAG TTCAGAATGG 
CTGGGGGTCC TGAAGGCTCT TTGTTGTTCT TCAAATCATG TGTGGGGCTT TAATGATGTT 
CTTTGCACTG TCGATGTGAG TGACCTTTCA TTCCATGATT CATTAGCTAC TTTCATTGCT 
ATTCTGATAG CACGACAGTG TTTCTCCCTG GAGGACGTCG TGCAACACGT CGCACTCCCC 
TCTCTCCTCG CCGCAGGCAA ACCTTTCCCG GGAATAAGAT CGTCTTGTGA CAGACACCTC 
TTAGCTGCTG CTCACAACAG CATTGAAGTG GGAGCCGTGT TTGCTGTCTT AAAAGCAATT 
ATGATGCTTG GAGATGCCAA AATTGGCAAT AACAGTGTCA GCTCTTTAAA AAATGATGAC 
TTCACCATGA GGGGTTTACG ACGTGATGGG AATGCTGAGG ATATCTGGAC TGCCTCACAA 
AATTCAAAAT CCTGTGGGAA AAGCATTTCC ATAGAAACGG CCAATTTAAG AGAATATGCT 
AGATATGTAC TGAGGACCAT CTGTCAACAG GAATGGGTAG GAGAACATTG TTTAAAAGAA 
CCTGAGAGAT TATGCACAGA CAAAGAACTT ATTCTGGACC CTGTGCTCTC AAATATGCAA 
GCACAGAAAT TACTGCAGCT GATCTGTTAT CCTCATGGCA TTAAGGAATG TACGGAGGGG 
GACAACCTCC AAAGACAGCA CATTAAACGC ATTCTTCAGA ATCTTGAGCA GTGGACGCTG 
AGGCAGTCCT GGCTGGAGCT TCAGTTAATG ATCAAACAGT GCTTGAAGGA CCCTGGCTCT 
GGTTCGGTGG CTGAAATGAA CAACCTGCTG GACAATATTG CCAAGGCAAC AATAGAGGTG 
TTCCAGCAGT CCGCTGACCT AAACAATAAC TCTTCTAATG CTGGCATGGG CCTCTTCAAC 
CCAAATGGAA TTGGAAGTAC TGATACAAGT AGCACGAGAC AGAATGGAAT AAAGACATTT 
CTAAGTTCCT CTGAACGCAG GGGTGTGTGG CTGGTGGCCC CCCTAATTGC CAGGCTGCCG 
ACTTCTGTGC AGGGAAGAGT GTTGAAAGCA GCTGGGGAAG AGCTGGAGAA AGGACAGCAC 
TTGGGTTCTT CTTCCAAAAA GGAAAGAGAC AGACAAAAGC AGAAAAGTAT GTCTCTTTTG 
AGTCAACAAC CATTCCTCTC ACTGGTCCTT ACCTGCCTTA AGGGACAAGA TGAACAACGA 
GAAGGCCTTC TAACATCTCT GCAGAATCAA GTTAACCAGA TTTTAAGTAA CTGGAGAGAA 
GAACGATACC AGGATGACAT CAAAGCACGA CAGATGATGC ATGAGGCATT GCAACTCCGC 
CTGAATTTGG TGGGAGGAAT GTTTGACACA GTGCAGAGGA GCACCCAGTG GACAACAGAC 
TGGGCGCTTC TCCTCCTTCA GATCATTACT TCTGGAACCG TTGACATGCA CACCAACAAT 
GAATTATTCA CAACAGTTCT TGACATGCTG GGTGTTTTAA TCAATGGAAC ATTGGCCTCT 
GACCTGTCGA ATGCATCCCC AGGGGGATCT GAAGAGAACA AACGTGCATA CATGAACTTA 
GTCAAGAAAC TGAAAAAAGA GCTGGGGGAC AAGCGGTCAG AAAGTATTGA CAAAGTTCGA 
CAGCTGTTAC CTTTGCCGAA ACAGACGTGT GACATCATTA CCTGTGAACC TATGGGCTCC 
TTGATTGACA CAAAGGGAAA CAAAATTGCT GGATTCGATT CTATAGATAA AAAACAGGGT 
CTCCAGGTCT CCACGAAGCA GAAGGTGTCC CCGTGGGATT TGTTTGAGGG TCAGAAGAAC 
CCGGCTCCTC TGTCCTGGGC CTGGTTCGGG ACTGTCCGAG TGGACCGGAA GGTGATCAAG 
TATGAGGAGC AACATCACCT CCTCCTGTAT CACACGCACC CCACGCCCAA GCCCCGGAGT 
TACTACCTCG AGCCACTGCC CCTGCCTCCC GAAGAGGAAG AGGAAGAGCC CACATCTCCC 
ATCTCTCAGG AACCTGAGAG GAAATCAGCT GAGCTGTCAG ATCAGGGAAA AACCGCAACA 
GATGAAGAGA AGAAGACAAA GGGAAGGAAG CGCAAGACAA AGTCCAGCTC AAGGGTTGAT 
GAGTATCCGC AGAGCAGCAT GTACCGAGTG CCTCCCAACT ACTCACCCAT CTCCTCCCAG 
GTGATGCACC ACCCGCAGTC CGCCCTGTGG GGCTACAACC TCATGGGTCA GCCCCAGCAG 
CCCGGCTTCT TCCTTCAGAA CCAGTCTGTC ACTCCAGGTG GCTCCCGGTT GGACCCCGCT 
GGCTCCTTCG TCCCCACCAA CACCAAGCAA GCTTTGTCCA ACATGCTCCA GCGGCGCTCG 
GGGGCCATGA TGCAGCCGCC CTCTCTGCAT GCCATCACAT CGCAGCAGCA GTTGATACAG 
ATGAAGCTTC TGCAGCAGCA GCAGCAACAG CGGCTTCTCA GGCAAGCCCA GACGCGGCCT 
TTCCAACAGG GCCAGCCAGG GGACCAGGCT GCTCTCTTTG CTGCTCAGGC ACGGCCCTCC 
CCTCAGCTCC CCCAGTATCC AGGACTGCAG CAAGCACAGA CCATGCCCCA GGGCTACACA 
ATGTATGGAA CCCAGATGCC TTTGCAGCAG ACCCCTCAGC AGCAGGCTGG CAGCGTGGTC 
CTGTCTCCTA CCTATAATTC CAGAACCTAT CCGGCCGCTC ACTCCAACCC AGCGCTCATG 
GAAAGACTCA GACAGATGCA GCAGCCGCCG AGCGGCTATG TTCAGCAGCA GGCGTCACCA 
TACCTGCATC CCCTGACCGG CTCCCAGAGG CTGAATCATC AGTCGCTTCA GCAGAGCCCT 
CTGGTGGGTG GAGGAATCGA TGCGGTGCTG ACTTCCGCAC ATCCAAACCT TCCCTCAGTG 
CCCTTGTCTC AGGACCCGAT GAGACCCAGA CAGCCACAGG TTCGACAGCA GCAGAGGCTT 
CTCCAGATGC AGCAGCCCCA GCAGCCCCCC CAGCCCCAGC AGCCTCCCCA GCCTCAGGGG 
TCCTCGCAGA CCCAGAGTCA GACCCTTGGT CTCCAAACAA TGCAGCCCCA GCAGCCTCTG 
TTCCCCAGGC AAGGCTTGCA GCAGACCCAG CAGCAACAGC AGACGGCTGC CCTGGTGCGG 
CAGCTCCAGA AGCAGCTTTC CAGTAACCAG CCACAGCAAG GAGTGACTCC CTACGGGCAT 
CCTTCACACT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_024990956.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846724.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X7, mRNA.

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CloneID	OBa218865
Clone ID Related Accession (Same CDS sequence)	XM_024990956.1
Accession Version	XM_024990956.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTGCCCCCA ACGATGAAGC TGTGGTGACG 
CTGCTGTGTG AATGGGCTGT GAGCTGTAAG CGGTCAGGCA AGCACAGGGC GATGGCTGTG 
GCGAAACTCC TGGAGAAGAG ACAAGCGGAA ATCGAGGCAG AGAGATGTGG TGAATCAGAA 
GTCTTAGATG AGAAGGAGTC CATTTCCTCA GCCTCCCTCG CTGGATCTAG TTTACCTGTT 
TTCCAGAATG TTCTTCTAAG GTTTTTAGAT ACACAGGCCC CCTCGTTGTC GGATCCAAAT 
AGCGAATGTG AGAAGGTGGA GTTTGTCAAC CTGGTGCTGC TCTTCTGTGA GTTCATCCGG 
CATGACGTCT TCTCCCACGA TGCCTACATG TGCACCCTCA TCTCTCGGGG AGACCTGTCT 
GTCACCGCCT CCACGCGACC GCGGTCGCCA GCAGGAGAGA ATACGGATGA GCATTATCCA 
AAGGACCACG ACATGAAAAT GGAGATTTTT TCTCCCATGC CTGGAGAGTC CTGTGAGAAT 
GCCAACCCTT CTCTGGGCAG AAGAATGTCA GTTAATGGTG AGAAGCTGTT GAAGAGAGAA 
AAACCAAGGG AGTTGATTTT TCCATCTAAT TATGACCTCC TACGACACCT GCAGTATGCA 
ACCCATTTTC CCATACCTCT GGATGAATCT TCAAGTCATG AATGTAACCA GCGCACAATC 
CTTCTTTATG GAGTGGGAAA AGAGCGTGAC GAAGCCAGAC ATCAGCTGAA GAAGATTACC 
AAAGATATTT TGAAAATCCT AAATAAGAAG AGCAACACAG AGACAGGGGT TGGAGATGAA 
GGACAGAAAG CCAGGAAGAG CAAACAGGAA GCATTTCCGA CCCTGGAGAC TGTGTTCACA 
AAACTTCAAC TCCTTTCATA TTTTGATCAG CATCAAGTGA CATCTCAGAT TTCTAACAAT 
GTGCTAGAAC AAATCACAAG CTTTGCATCA GGAACATCAT ACCATCTCCC ATTGGCTCAC 
CACATTCAGC TCATCTTTGA TCTCATGGAG CCAGCACTTA ATATTAATGG ACTCATTGAC 
TTCGCAATAC AGCTCCTGAA CGAACTGAGT GTTGTGGAGG CAGAACTGCT CCTGAAGTCC 
TCCAGCCTGG CAGGAAGCTA CACGACAGGA CTGTGTGTCT GCATCGTGGC TGTTCTCAGG 
CGTTACCACA GTTGTCTGAT CCTGAATCCT GATCAGACAG CCCAGGTGTT TGAAGGATTG 
TGTGGTGTGG TAAAGCACGT TGTGAACCCC TCAGAGTGCT CTTCCCCCGA AAGATGCATC 
TTAGCCTACC TCTACGACCT CTACGTGTCA TGTAGCCACC TCAGAAGTAA ATTTGGAGAC 
CTCTTCAGTA GTGCCTGTTC AAAAGTAAAG CAAACCATAT ATAACAATGT GATTCCTGCA 
AATTCTAACT TGCGATGGGA TCCAGACTTC ATGATGGATT TTATTGAGAA TCCCTCGGCC 
CGTAGCGTCA ACTACTCAGT GCTGGGCAAG ATCCTTAGTG ACAATGCAGC CAATCGCTAC 
AGCTTCGTCT GCAACACTCT CATGAATGTA TGTATGGGTC ATCAGGATGC TGGAAGGATT 
AATGACATAG CCAATTTCTC CTCCGAGCTG ACAGCCTGCT GCACTGTTCT CAGTTCAGAA 
TGGCTGGGGG TCCTGAAGGC TCTTTGTTGT TCTTCAAATC ATGTGTGGGG CTTTAATGAT 
GTTCTTTGCA CTGTCGATGT GAGTGACCTT TCATTCCATG ATTCATTAGC TACTTTCATT 
GCTATTCTGA TAGCACGACA GTGTTTCTCC CTGGAGGACG TCGTGCAACA CGTCGCACTC 
CCCTCTCTCC TCGCCGCAGC TTGCGGAGAT GCAGATGCAG AGCCTGGGGC AAGAATGACA 
TGCCGTCTCC TGCTTCATCT CTTTCGAGCT CCCCAGGCCT GCCTGTTACC TCAAGCAACT 
GGCAAACCTT TCCCGGGAAT AAGATCGTCT TGTGACAGAC ACCTCTTAGC TGCTGCTCAC 
AACAGCATTG AAGTGGGAGC CGTGTTTGCT GTCTTAAAAG CAATTATGAT GCTTGGAGAT 
GCCAAAATTG GCAATAACAG TGTCAGCTCT TTAAAAAATG ATGACTTCAC CATGAGGGGT 
TTACGACGTG ATGGGAATGC TGAGGATATC TGGACTGCCT CACAAAATTC AAAATCCTGT 
GGGAAAAGCA TTTCCATAGA AACGGCCAAT TTAAGAGAAT ATGCTAGATA TGTACTGAGG 
ACCATCTGTC AACAGGAATG GGTAGGAGAA CATTGTTTAA AAGAACCTGA GAGATTATGC 
ACAGACAAAG AACTTATTCT GGACCCTGTG CTCTCAAATA TGCAAGCACA GAAATTACTG 
CAGCTGATCT GTTATCCTCA TGGCATTAAG GAATGTACGG AGGGGGACAA CCTCCAAAGA 
CAGCACATTA AACGCATTCT TCAGAATCTT GAGCAGTGGA CGCTGAGGCA GTCCTGGCTG 
GAGCTTCAGT TAATGATCAA ACAGTGCTTG AAGGACCCTG GCTCTGGTTC GGTGGCTGAA 
ATGAACAACC TGCTGGACAA TATTGCCAAG GCAACAATAG AGGTGTTCCA GCAGTCCGCT 
GACCTAAACA ATAACTCTTC TAATGCTGGC ATGGGCCTCT TCAACCCAAA TGGAATTGGA 
AGTACTGATA CAAGTAGCAC GAGACAGAAT GGAATAAAGA CATTTCTAAG TTCCTCTGAA 
CGCAGGGGTG TGTGGCTGGT GGCCCCCCTA ATTGCCAGGC TGCCGACTTC TGTGCAGGGA 
AGAGTGTTGA AAGCAGCTGG GGAAGAGCTG GAGAAAGGAC AGCACTTGGG TTCTTCTTCC 
AAAAAGGAAA GAGACAGACA AAAGCAGAAA AGTATGTCTC TTTTGAGTCA ACAACCATTC 
CTCTCACTGG TCCTTACCTG CCTTAAGGGA CAAGATGAAC AACGAGAAGG CCTTCTAACA 
TCTCTGCAGA ATCAAGTTAA CCAGATTTTA AGTAACTGGA GAGAAGAACG ATACCAGGAT 
GACATCAAAG CACGACAGAT GATGCATGAG GCATTGCAAC TCCGCCTGAA TTTGGTGGGA 
GGAATGTTTG ACACAGTGCA GAGGAGCACC CAGTGGACAA CAGACTGGGC GCTTCTCCTC 
CTTCAGATCA TTACTTCTGG AACCGTTGAC ATGCACACCA ACAATGAATT ATTCACAACA 
GTTCTTGACA TGCTGGGTGT TTTAATCAAT GGAACATTGG CCTCTGACCT GTCGAATGCA 
TCCCCAGGGG GATCTGAAGA GAACAAACGT GCATACATGA ACTTAGTCAA GAAACTGAAA 
AAAGAGCTGG GGGACAAGCG GTCAGAAAGT ATTGACAAAG TTCGACAGCT GTTACCTTTG 
CCGAAACAGA CGTGTGACAT CATTACCTGT GAACCTATGG GCTCCTTGAT TGACACAAAG 
GGAAACAAAA TTGCTGGATT CGATTCTATA GATAAAAAAC AGGGTCTCCA GGTCTCCACG 
AAGCAGAAGG TGTCCCCGTG GGATTTGTTT GAGGGTCAGA AGAACCCGGC TCCTCTGTCC 
TGGGCCTGGT TCGGGACTGT CCGAGTGGAC CGGAAGGTGA TCAAGTATGA GGAGCAACAT 
CACCTCCTCC TGTATCACAC GCACCCCACG CCCAAGCCCC GGAGTTACTA CCTCGAGCCA 
CTGCCCCTGC CTCCCGAAGA GGAAGAGGAA GAGCCCACAT CTCCCATCTC TCAGGAACCT 
GAGAGGAAAT CAGCTGAGCT GTCAGATCAG GGAAAAACCG CAACAGATGA AGAGAAGAAG 
ACAAAGGGAA GGAAGCGCAA GACAAAGTCC AGCTCAAGGG TTGATGAGTA TCCGCAGAGC 
AGCATGTACC GAGTGCCTCC CAACTACTCA CCCATCTCCT CCCAGGTGAT GCACCACCCG 
CAGTCCGCCC TGTGGGGCTA CAACCTCATG GGTCAGCCCC AGCAGCCCGG CTTCTTCCTT 
CAGAACCAGT CTGTCACTCC AGGTGGCTCC CGGTTGGACC CCGCTGGCTC CTTCGTCCCC 
ACCAACACCA AGCAAGCTTT GTCCAACATG CTCCAGCGGC GCTCGGGGGC CATGATGCAG 
CCGCCCTCTC TGCATGCCAT CACATCGCAG CAGCAGTTGA TACAGATGAA GCTTCTGCAG 
CAGCAGCAGC AACAGCGGCT TCTCAGGCAA GCCCAGACGC GGCCTTTCCA ACAGGGCCAG 
CCAGGGGACC AGGCTGCTCT CTTTGCTGCT CAGGCACGGC CCTCCCCTCA GCTCCCCCAG 
TATCCAGGAC TGCAGCAAGC ACAGACCATG CCCCAGGGCT ACACAATGTA TGGAACCCAG 
ATGCCTTTGC AGCAGACCCC TCAGCAGCAG GCTGGCAGCG TGGTCCTGTC TCCTACCTAT 
AATTCCAGAA CCTATCCGGC CGCTCACTCC AACCCAGCGC TCATGGAAAG ACTCAGACAG 
ATGCAGCAGC CGCCGAGCGG CTATGTTCAG CAGCAGGCGT CACCATACCT GCATCCCCTG 
ACCGGCTCCC AGAGGCTGAA TCATCAGTCG CTTCAGCAGA GCCCTCTGGT GGGTGGAGGA 
ATCGATGCGG TGCTGACTTC CGCACATCCA AACCTTCCCT CAGTGCCCTT GTCTCAGGAC 
CCGATGAGAC CCAGACAGCC ACAGGTTCGA CAGCAGCAGA GGCTTCTCCA GATGCAGCAG 
CCCCAGCAGC CCCCCCAGCC CCAGCAGCCT CCCCAGCCTC AGGGGTCCTC GCAGACCCAG 
AGTCAGACCC TTGGTCTCCA AACAATGCAG CCCCAGCAGC CTCTGTTCCC CAGGCAAGGC 
TTGCAGCAGA CCCAGCAGCA ACAGCAGACG GCTGCCCTGG TGCGGCAGCT CCAGAAGCAG 
CTTTCCAGTA ACCAGCCACA GCAAGGAGTG ACTCCCTACG GGCATCCTTC ACACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846724.1
CDS	414..6653
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_024990956.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990956.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTGCCCCCA ACGATGAAGC TGTGGTGACG 
CTGCTGTGTG AATGGGCTGT GAGCTGTAAG CGGTCAGGCA AGCACAGGGC GATGGCTGTG 
GCGAAACTCC TGGAGAAGAG ACAAGCGGAA ATCGAGGCAG AGAGATGTGG TGAATCAGAA 
GTCTTAGATG AGAAGGAGTC CATTTCCTCA GCCTCCCTCG CTGGATCTAG TTTACCTGTT 
TTCCAGAATG TTCTTCTAAG GTTTTTAGAT ACACAGGCCC CCTCGTTGTC GGATCCAAAT 
AGCGAATGTG AGAAGGTGGA GTTTGTCAAC CTGGTGCTGC TCTTCTGTGA GTTCATCCGG 
CATGACGTCT TCTCCCACGA TGCCTACATG TGCACCCTCA TCTCTCGGGG AGACCTGTCT 
GTCACCGCCT CCACGCGACC GCGGTCGCCA GCAGGAGAGA ATACGGATGA GCATTATCCA 
AAGGACCACG ACATGAAAAT GGAGATTTTT TCTCCCATGC CTGGAGAGTC CTGTGAGAAT 
GCCAACCCTT CTCTGGGCAG AAGAATGTCA GTTAATGGTG AGAAGCTGTT GAAGAGAGAA 
AAACCAAGGG AGTTGATTTT TCCATCTAAT TATGACCTCC TACGACACCT GCAGTATGCA 
ACCCATTTTC CCATACCTCT GGATGAATCT TCAAGTCATG AATGTAACCA GCGCACAATC 
CTTCTTTATG GAGTGGGAAA AGAGCGTGAC GAAGCCAGAC ATCAGCTGAA GAAGATTACC 
AAAGATATTT TGAAAATCCT AAATAAGAAG AGCAACACAG AGACAGGGGT TGGAGATGAA 
GGACAGAAAG CCAGGAAGAG CAAACAGGAA GCATTTCCGA CCCTGGAGAC TGTGTTCACA 
AAACTTCAAC TCCTTTCATA TTTTGATCAG CATCAAGTGA CATCTCAGAT TTCTAACAAT 
GTGCTAGAAC AAATCACAAG CTTTGCATCA GGAACATCAT ACCATCTCCC ATTGGCTCAC 
CACATTCAGC TCATCTTTGA TCTCATGGAG CCAGCACTTA ATATTAATGG ACTCATTGAC 
TTCGCAATAC AGCTCCTGAA CGAACTGAGT GTTGTGGAGG CAGAACTGCT CCTGAAGTCC 
TCCAGCCTGG CAGGAAGCTA CACGACAGGA CTGTGTGTCT GCATCGTGGC TGTTCTCAGG 
CGTTACCACA GTTGTCTGAT CCTGAATCCT GATCAGACAG CCCAGGTGTT TGAAGGATTG 
TGTGGTGTGG TAAAGCACGT TGTGAACCCC TCAGAGTGCT CTTCCCCCGA AAGATGCATC 
TTAGCCTACC TCTACGACCT CTACGTGTCA TGTAGCCACC TCAGAAGTAA ATTTGGAGAC 
CTCTTCAGTA GTGCCTGTTC AAAAGTAAAG CAAACCATAT ATAACAATGT GATTCCTGCA 
AATTCTAACT TGCGATGGGA TCCAGACTTC ATGATGGATT TTATTGAGAA TCCCTCGGCC 
CGTAGCGTCA ACTACTCAGT GCTGGGCAAG ATCCTTAGTG ACAATGCAGC CAATCGCTAC 
AGCTTCGTCT GCAACACTCT CATGAATGTA TGTATGGGTC ATCAGGATGC TGGAAGGATT 
AATGACATAG CCAATTTCTC CTCCGAGCTG ACAGCCTGCT GCACTGTTCT CAGTTCAGAA 
TGGCTGGGGG TCCTGAAGGC TCTTTGTTGT TCTTCAAATC ATGTGTGGGG CTTTAATGAT 
GTTCTTTGCA CTGTCGATGT GAGTGACCTT TCATTCCATG ATTCATTAGC TACTTTCATT 
GCTATTCTGA TAGCACGACA GTGTTTCTCC CTGGAGGACG TCGTGCAACA CGTCGCACTC 
CCCTCTCTCC TCGCCGCAGC TTGCGGAGAT GCAGATGCAG AGCCTGGGGC AAGAATGACA 
TGCCGTCTCC TGCTTCATCT CTTTCGAGCT CCCCAGGCCT GCCTGTTACC TCAAGCAACT 
GGCAAACCTT TCCCGGGAAT AAGATCGTCT TGTGACAGAC ACCTCTTAGC TGCTGCTCAC 
AACAGCATTG AAGTGGGAGC CGTGTTTGCT GTCTTAAAAG CAATTATGAT GCTTGGAGAT 
GCCAAAATTG GCAATAACAG TGTCAGCTCT TTAAAAAATG ATGACTTCAC CATGAGGGGT 
TTACGACGTG ATGGGAATGC TGAGGATATC TGGACTGCCT CACAAAATTC AAAATCCTGT 
GGGAAAAGCA TTTCCATAGA AACGGCCAAT TTAAGAGAAT ATGCTAGATA TGTACTGAGG 
ACCATCTGTC AACAGGAATG GGTAGGAGAA CATTGTTTAA AAGAACCTGA GAGATTATGC 
ACAGACAAAG AACTTATTCT GGACCCTGTG CTCTCAAATA TGCAAGCACA GAAATTACTG 
CAGCTGATCT GTTATCCTCA TGGCATTAAG GAATGTACGG AGGGGGACAA CCTCCAAAGA 
CAGCACATTA AACGCATTCT TCAGAATCTT GAGCAGTGGA CGCTGAGGCA GTCCTGGCTG 
GAGCTTCAGT TAATGATCAA ACAGTGCTTG AAGGACCCTG GCTCTGGTTC GGTGGCTGAA 
ATGAACAACC TGCTGGACAA TATTGCCAAG GCAACAATAG AGGTGTTCCA GCAGTCCGCT 
GACCTAAACA ATAACTCTTC TAATGCTGGC ATGGGCCTCT TCAACCCAAA TGGAATTGGA 
AGTACTGATA CAAGTAGCAC GAGACAGAAT GGAATAAAGA CATTTCTAAG TTCCTCTGAA 
CGCAGGGGTG TGTGGCTGGT GGCCCCCCTA ATTGCCAGGC TGCCGACTTC TGTGCAGGGA 
AGAGTGTTGA AAGCAGCTGG GGAAGAGCTG GAGAAAGGAC AGCACTTGGG TTCTTCTTCC 
AAAAAGGAAA GAGACAGACA AAAGCAGAAA AGTATGTCTC TTTTGAGTCA ACAACCATTC 
CTCTCACTGG TCCTTACCTG CCTTAAGGGA CAAGATGAAC AACGAGAAGG CCTTCTAACA 
TCTCTGCAGA ATCAAGTTAA CCAGATTTTA AGTAACTGGA GAGAAGAACG ATACCAGGAT 
GACATCAAAG CACGACAGAT GATGCATGAG GCATTGCAAC TCCGCCTGAA TTTGGTGGGA 
GGAATGTTTG ACACAGTGCA GAGGAGCACC CAGTGGACAA CAGACTGGGC GCTTCTCCTC 
CTTCAGATCA TTACTTCTGG AACCGTTGAC ATGCACACCA ACAATGAATT ATTCACAACA 
GTTCTTGACA TGCTGGGTGT TTTAATCAAT GGAACATTGG CCTCTGACCT GTCGAATGCA 
TCCCCAGGGG GATCTGAAGA GAACAAACGT GCATACATGA ACTTAGTCAA GAAACTGAAA 
AAAGAGCTGG GGGACAAGCG GTCAGAAAGT ATTGACAAAG TTCGACAGCT GTTACCTTTG 
CCGAAACAGA CGTGTGACAT CATTACCTGT GAACCTATGG GCTCCTTGAT TGACACAAAG 
GGAAACAAAA TTGCTGGATT CGATTCTATA GATAAAAAAC AGGGTCTCCA GGTCTCCACG 
AAGCAGAAGG TGTCCCCGTG GGATTTGTTT GAGGGTCAGA AGAACCCGGC TCCTCTGTCC 
TGGGCCTGGT TCGGGACTGT CCGAGTGGAC CGGAAGGTGA TCAAGTATGA GGAGCAACAT 
CACCTCCTCC TGTATCACAC GCACCCCACG CCCAAGCCCC GGAGTTACTA CCTCGAGCCA 
CTGCCCCTGC CTCCCGAAGA GGAAGAGGAA GAGCCCACAT CTCCCATCTC TCAGGAACCT 
GAGAGGAAAT CAGCTGAGCT GTCAGATCAG GGAAAAACCG CAACAGATGA AGAGAAGAAG 
ACAAAGGGAA GGAAGCGCAA GACAAAGTCC AGCTCAAGGG TTGATGAGTA TCCGCAGAGC 
AGCATGTACC GAGTGCCTCC CAACTACTCA CCCATCTCCT CCCAGGTGAT GCACCACCCG 
CAGTCCGCCC TGTGGGGCTA CAACCTCATG GGTCAGCCCC AGCAGCCCGG CTTCTTCCTT 
CAGAACCAGT CTGTCACTCC AGGTGGCTCC CGGTTGGACC CCGCTGGCTC CTTCGTCCCC 
ACCAACACCA AGCAAGCTTT GTCCAACATG CTCCAGCGGC GCTCGGGGGC CATGATGCAG 
CCGCCCTCTC TGCATGCCAT CACATCGCAG CAGCAGTTGA TACAGATGAA GCTTCTGCAG 
CAGCAGCAGC AACAGCGGCT TCTCAGGCAA GCCCAGACGC GGCCTTTCCA ACAGGGCCAG 
CCAGGGGACC AGGCTGCTCT CTTTGCTGCT CAGGCACGGC CCTCCCCTCA GCTCCCCCAG 
TATCCAGGAC TGCAGCAAGC ACAGACCATG CCCCAGGGCT ACACAATGTA TGGAACCCAG 
ATGCCTTTGC AGCAGACCCC TCAGCAGCAG GCTGGCAGCG TGGTCCTGTC TCCTACCTAT 
AATTCCAGAA CCTATCCGGC CGCTCACTCC AACCCAGCGC TCATGGAAAG ACTCAGACAG 
ATGCAGCAGC CGCCGAGCGG CTATGTTCAG CAGCAGGCGT CACCATACCT GCATCCCCTG 
ACCGGCTCCC AGAGGCTGAA TCATCAGTCG CTTCAGCAGA GCCCTCTGGT GGGTGGAGGA 
ATCGATGCGG TGCTGACTTC CGCACATCCA AACCTTCCCT CAGTGCCCTT GTCTCAGGAC 
CCGATGAGAC CCAGACAGCC ACAGGTTCGA CAGCAGCAGA GGCTTCTCCA GATGCAGCAG 
CCCCAGCAGC CCCCCCAGCC CCAGCAGCCT CCCCAGCCTC AGGGGTCCTC GCAGACCCAG 
AGTCAGACCC TTGGTCTCCA AACAATGCAG CCCCAGCAGC CTCTGTTCCC CAGGCAAGGC 
TTGCAGCAGA CCCAGCAGCA ACAGCAGACG GCTGCCCTGG TGCGGCAGCT CCAGAAGCAG 
CTTTCCAGTA ACCAGCCACA GCAAGGAGTG ACTCCCTACG GGCATCCTTC ACACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_024990957.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846725.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X8, mRNA.

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CloneID	OBa218866
Clone ID Related Accession (Same CDS sequence)	XM_024990957.1
Accession Version	XM_024990957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAGTCCTG TGAGAATGCC 
AACCCTTCTC TGGGCAGAAG AATGTCAGTT AATGGTGAGA AGCTGTTGAA GAGAGAAAAA 
CCAAGGGAGT TGATTTTTCC ATCTAATTAT GACCTCCTAC GACACCTGCA GTATGCAACC 
CATTTTCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTT 
CTTTATGGAG TGGGAAAAGA GCGTGACGAA GCCAGACATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC AACACAGAGA CAGGGGTTGG AGATGAAGGA 
CAGAAAGCCA GGAAGAGCAA ACAGGAAGCA TTTCCGACCC TGGAGACTGT GTTCACAAAA 
CTTCAACTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATTTC TAACAATGTG 
CTAGAACAAA TCACAAGCTT TGCATCAGGA ACATCATACC ATCTCCCATT GGCTCACCAC 
ATTCAGCTCA TCTTTGATCT CATGGAGCCA GCACTTAATA TTAATGGACT CATTGACTTC 
GCAATACAGC TCCTGAACGA ACTGAGTGTT GTGGAGGCAG AACTGCTCCT GAAGTCCTCC 
AGCCTGGCAG GAAGCTACAC GACAGGACTG TGTGTCTGCA TCGTGGCTGT TCTCAGGCGT 
TACCACAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGATTGTGT 
GGTGTGGTAA AGCACGTTGT GAACCCCTCA GAGTGCTCTT CCCCCGAAAG ATGCATCTTA 
GCCTACCTCT ACGACCTCTA CGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTAGTG CCTGTTCAAA AGTAAAGCAA ACCATATATA ACAATGTGAT TCCTGCAAAT 
TCTAACTTGC GATGGGATCC AGACTTCATG ATGGATTTTA TTGAGAATCC CTCGGCCCGT 
AGCGTCAACT ACTCAGTGCT GGGCAAGATC CTTAGTGACA ATGCAGCCAA TCGCTACAGC 
TTCGTCTGCA ACACTCTCAT GAATGTATGT ATGGGTCATC AGGATGCTGG AAGGATTAAT 
GACATAGCCA ATTTCTCCTC CGAGCTGACA GCCTGCTGCA CTGTTCTCAG TTCAGAATGG 
CTGGGGGTCC TGAAGGCTCT TTGTTGTTCT TCAAATCATG TGTGGGGCTT TAATGATGTT 
CTTTGCACTG TCGATGTGAG TGACCTTTCA TTCCATGATT CATTAGCTAC TTTCATTGCT 
ATTCTGATAG CACGACAGTG TTTCTCCCTG GAGGACGTCG TGCAACACGT CGCACTCCCC 
TCTCTCCTCG CCGCAGCTTG CGGAGATGCA GATGCAGAGC CTGGGGCAAG AATGACATGC 
CGTCTCCTGC TTCATCTCTT TCGAGCTCCC CAGGCCTGCC TGTTACCTCA AGCAACTGGC 
AAACCTTTCC CGGGAATAAG ATCGTCTTGT GACAGACACC TCTTAGCTGC TGCTCACAAC 
AGCATTGAAG TGGGAGCCGT GTTTGCTGTC TTAAAAGCAA TTATGATGCT TGGAGATGCC 
AAAATTGGCA ATAACAGTGT CAGCTCTTTA AAAAATGATG ACTTCACCAT GAGGGGTTTA 
CGACGTGATG GGAATGCTGA GGATATCTGG ACTGCCTCAC AAAATTCAAA ATCCTGTGGG 
AAAAGCATTT CCATAGAAAC GGCCAATTTA AGAGAATATG CTAGATATGT ACTGAGGACC 
ATCTGTCAAC AGGAATGGGT AGGAGAACAT TGTTTAAAAG AACCTGAGAG ATTATGCACA 
GACAAAGAAC TTATTCTGGA CCCTGTGCTC TCAAATATGC AAGCACAGAA ATTACTGCAG 
CTGATCTGTT ATCCTCATGG CATTAAGGAA TGTACGGAGG GGGACAACCT CCAAAGACAG 
CACATTAAAC GCATTCTTCA GAATCTTGAG CAGTGGACGC TGAGGCAGTC CTGGCTGGAG 
CTTCAGTTAA TGATCAAACA GTGCTTGAAG GACCCTGGCT CTGGTTCGGT GGCTGAAATG 
AACAACCTGC TGGACAATAT TGCCAAGGCA ACAATAGAGG TGTTCCAGCA GTCCGCTGAC 
CTAAACAATA ACTCTTCTAA TGCTGGCATG GGCCTCTTCA ACCCAAATGG AATTGGAAGT 
ACTGATACAA GTAGCACGAG ACAGAATGGA ATAAAGACAT TTCTAAGTTC CTCTGAACGC 
AGGGGTGTGT GGCTGGTGGC CCCCCTAATT GCCAGGCTGC CGACTTCTGT GCAGGGAAGA 
GTGTTGAAAG CAGCTGGGGA AGAGCTGGAG AAAGGACAGC ACTTGGGTTC TTCTTCCAAA 
AAGGAAAGAG ACAGACAAAA GCAGAAAAGT ATGTCTCTTT TGAGTCAACA ACCATTCCTC 
TCACTGGTCC TTACCTGCCT TAAGGGACAA GATGAACAAC GAGAAGGCCT TCTAACATCT 
CTGCAGAATC AAGTTAACCA GATTTTAAGT AACTGGAGAG AAGAACGATA CCAGGATGAC 
ATCAAAGCAC GACAGATGAT GCATGAGGCA TTGCAACTCC GCCTGAATTT GGTGGGAGGA 
ATGTTTGACA CAGTGCAGAG GAGCACCCAG TGGACAACAG ACTGGGCGCT TCTCCTCCTT 
CAGATCATTA CTTCTGGAAC CGTTGACATG CACACCAACA ATGAATTATT CACAACAGTT 
CTTGACATGC TGGGTGTTTT AATCAATGGA ACATTGGCCT CTGACCTGTC GAATGCATCC 
CCAGGGGGAT CTGAAGAGAA CAAACGTGCA TACATGAACT TAGTCAAGAA ACTGAAAAAA 
GAGCTGGGGG ACAAGCGGTC AGAAAGTATT GACAAAGTTC GACAGCTGTT ACCTTTGCCG 
AAACAGACGT GTGACATCAT TACCTGTGAA CCTATGGGCT CCTTGATTGA CACAAAGGGA 
AACAAAATTG CTGGATTCGA TTCTATAGAT AAAAAACAGG GTCTCCAGGT CTCCACGAAG 
CAGAAGGTGT CCCCGTGGGA TTTGTTTGAG GGTCAGAAGA ACCCGGCTCC TCTGTCCTGG 
GCCTGGTTCG GGACTGTCCG AGTGGACCGG AAGGTGATCA AGTATGAGGA GCAACATCAC 
CTCCTCCTGT ATCACACGCA CCCCACGCCC AAGCCCCGGA GTTACTACCT CGAGCCACTG 
CCCCTGCCTC CCGAAGAGGA AGAGGAAGAG CCCACATCTC CCATCTCTCA GGAACCTGAG 
AGGAAATCAG CTGAGCTGTC AGATCAGGGA AAAACCGCAA CAGATGAAGA GAAGAAGACA 
AAGGGAAGGA AGCGCAAGAC AAAGTCCAGC TCAAGGGTTG ATGAGTATCC GCAGAGCAGC 
ATGTACCGAG TGCCTCCCAA CTACTCACCC ATCTCCTCCC AGGTGATGCA CCACCCGCAG 
TCCGCCCTGT GGGGCTACAA CCTCATGGGT CAGCCCCAGC AGCCCGGCTT CTTCCTTCAG 
AACCAGTCTG TCACTCCAGG TGGCTCCCGG TTGGACCCCG CTGGCTCCTT CGTCCCCACC 
AACACCAAGC AAGCTTTGTC CAACATGCTC CAGCGGCGCT CGGGGGCCAT GATGCAGCCG 
CCCTCTCTGC ATGCCATCAC ATCGCAGCAG CAGTTGATAC AGATGAAGCT TCTGCAGCAG 
CAGCAGCAAC AGCGGCTTCT CAGGCAAGCC CAGACGCGGC CTTTCCAACA GGGCCAGCCA 
GGGGACCAGG CTGCTCTCTT TGCTGCTCAG GCACGGCCCT CCCCTCAGCT CCCCCAGTAT 
CCAGGACTGC AGCAAGCACA GGCTGAATCA TCAGTCGCTT CAGCAGAGCC CTCTGGTGGG 
TGGAGGAATC GATGCGGTGC TGACTTCCGC ACATCCAAAC CTTCCCTCAG TGCCCTTGTC 
TCAGGACCCG ATGAGACCCA GACAGCCACA GGTTCGACAG CAGCAGAGGC TTCTCCAGAT 
GCAGCAGCCC CAGCAGCCCC CCCAGCCCCA GCAGCCTCCC CAGCCTCAGG GGTCCTCGCA 
GACCCAGAGT CAGACCCTTG GTCTCCAAAC AATGCAGCCC CAGCAGCCTC TGTTCCCCAG 
GCAAGGCTTG CAGCAGACCC AGCAGCAACA GCAGACGGCT GCCCTGGTGC GGCAGCTCCA 
GAAGCAGCTT TCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846725.1
CDS	414..6611
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQAESSVASAEPSGGWRNRCGADFRTSKPSLSALVSGPDETQTATGSTAAEASPDAAAPAAPPAPAASPASGVLADPESDPWSPNNAAPAASVPQARLAADPAATADGCPGAAAPEAAFQ

Target ORF information:

RefSeq Version	XM_024990957.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990957.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAGTCCTG TGAGAATGCC 
AACCCTTCTC TGGGCAGAAG AATGTCAGTT AATGGTGAGA AGCTGTTGAA GAGAGAAAAA 
CCAAGGGAGT TGATTTTTCC ATCTAATTAT GACCTCCTAC GACACCTGCA GTATGCAACC 
CATTTTCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTT 
CTTTATGGAG TGGGAAAAGA GCGTGACGAA GCCAGACATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC AACACAGAGA CAGGGGTTGG AGATGAAGGA 
CAGAAAGCCA GGAAGAGCAA ACAGGAAGCA TTTCCGACCC TGGAGACTGT GTTCACAAAA 
CTTCAACTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATTTC TAACAATGTG 
CTAGAACAAA TCACAAGCTT TGCATCAGGA ACATCATACC ATCTCCCATT GGCTCACCAC 
ATTCAGCTCA TCTTTGATCT CATGGAGCCA GCACTTAATA TTAATGGACT CATTGACTTC 
GCAATACAGC TCCTGAACGA ACTGAGTGTT GTGGAGGCAG AACTGCTCCT GAAGTCCTCC 
AGCCTGGCAG GAAGCTACAC GACAGGACTG TGTGTCTGCA TCGTGGCTGT TCTCAGGCGT 
TACCACAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGATTGTGT 
GGTGTGGTAA AGCACGTTGT GAACCCCTCA GAGTGCTCTT CCCCCGAAAG ATGCATCTTA 
GCCTACCTCT ACGACCTCTA CGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTAGTG CCTGTTCAAA AGTAAAGCAA ACCATATATA ACAATGTGAT TCCTGCAAAT 
TCTAACTTGC GATGGGATCC AGACTTCATG ATGGATTTTA TTGAGAATCC CTCGGCCCGT 
AGCGTCAACT ACTCAGTGCT GGGCAAGATC CTTAGTGACA ATGCAGCCAA TCGCTACAGC 
TTCGTCTGCA ACACTCTCAT GAATGTATGT ATGGGTCATC AGGATGCTGG AAGGATTAAT 
GACATAGCCA ATTTCTCCTC CGAGCTGACA GCCTGCTGCA CTGTTCTCAG TTCAGAATGG 
CTGGGGGTCC TGAAGGCTCT TTGTTGTTCT TCAAATCATG TGTGGGGCTT TAATGATGTT 
CTTTGCACTG TCGATGTGAG TGACCTTTCA TTCCATGATT CATTAGCTAC TTTCATTGCT 
ATTCTGATAG CACGACAGTG TTTCTCCCTG GAGGACGTCG TGCAACACGT CGCACTCCCC 
TCTCTCCTCG CCGCAGCTTG CGGAGATGCA GATGCAGAGC CTGGGGCAAG AATGACATGC 
CGTCTCCTGC TTCATCTCTT TCGAGCTCCC CAGGCCTGCC TGTTACCTCA AGCAACTGGC 
AAACCTTTCC CGGGAATAAG ATCGTCTTGT GACAGACACC TCTTAGCTGC TGCTCACAAC 
AGCATTGAAG TGGGAGCCGT GTTTGCTGTC TTAAAAGCAA TTATGATGCT TGGAGATGCC 
AAAATTGGCA ATAACAGTGT CAGCTCTTTA AAAAATGATG ACTTCACCAT GAGGGGTTTA 
CGACGTGATG GGAATGCTGA GGATATCTGG ACTGCCTCAC AAAATTCAAA ATCCTGTGGG 
AAAAGCATTT CCATAGAAAC GGCCAATTTA AGAGAATATG CTAGATATGT ACTGAGGACC 
ATCTGTCAAC AGGAATGGGT AGGAGAACAT TGTTTAAAAG AACCTGAGAG ATTATGCACA 
GACAAAGAAC TTATTCTGGA CCCTGTGCTC TCAAATATGC AAGCACAGAA ATTACTGCAG 
CTGATCTGTT ATCCTCATGG CATTAAGGAA TGTACGGAGG GGGACAACCT CCAAAGACAG 
CACATTAAAC GCATTCTTCA GAATCTTGAG CAGTGGACGC TGAGGCAGTC CTGGCTGGAG 
CTTCAGTTAA TGATCAAACA GTGCTTGAAG GACCCTGGCT CTGGTTCGGT GGCTGAAATG 
AACAACCTGC TGGACAATAT TGCCAAGGCA ACAATAGAGG TGTTCCAGCA GTCCGCTGAC 
CTAAACAATA ACTCTTCTAA TGCTGGCATG GGCCTCTTCA ACCCAAATGG AATTGGAAGT 
ACTGATACAA GTAGCACGAG ACAGAATGGA ATAAAGACAT TTCTAAGTTC CTCTGAACGC 
AGGGGTGTGT GGCTGGTGGC CCCCCTAATT GCCAGGCTGC CGACTTCTGT GCAGGGAAGA 
GTGTTGAAAG CAGCTGGGGA AGAGCTGGAG AAAGGACAGC ACTTGGGTTC TTCTTCCAAA 
AAGGAAAGAG ACAGACAAAA GCAGAAAAGT ATGTCTCTTT TGAGTCAACA ACCATTCCTC 
TCACTGGTCC TTACCTGCCT TAAGGGACAA GATGAACAAC GAGAAGGCCT TCTAACATCT 
CTGCAGAATC AAGTTAACCA GATTTTAAGT AACTGGAGAG AAGAACGATA CCAGGATGAC 
ATCAAAGCAC GACAGATGAT GCATGAGGCA TTGCAACTCC GCCTGAATTT GGTGGGAGGA 
ATGTTTGACA CAGTGCAGAG GAGCACCCAG TGGACAACAG ACTGGGCGCT TCTCCTCCTT 
CAGATCATTA CTTCTGGAAC CGTTGACATG CACACCAACA ATGAATTATT CACAACAGTT 
CTTGACATGC TGGGTGTTTT AATCAATGGA ACATTGGCCT CTGACCTGTC GAATGCATCC 
CCAGGGGGAT CTGAAGAGAA CAAACGTGCA TACATGAACT TAGTCAAGAA ACTGAAAAAA 
GAGCTGGGGG ACAAGCGGTC AGAAAGTATT GACAAAGTTC GACAGCTGTT ACCTTTGCCG 
AAACAGACGT GTGACATCAT TACCTGTGAA CCTATGGGCT CCTTGATTGA CACAAAGGGA 
AACAAAATTG CTGGATTCGA TTCTATAGAT AAAAAACAGG GTCTCCAGGT CTCCACGAAG 
CAGAAGGTGT CCCCGTGGGA TTTGTTTGAG GGTCAGAAGA ACCCGGCTCC TCTGTCCTGG 
GCCTGGTTCG GGACTGTCCG AGTGGACCGG AAGGTGATCA AGTATGAGGA GCAACATCAC 
CTCCTCCTGT ATCACACGCA CCCCACGCCC AAGCCCCGGA GTTACTACCT CGAGCCACTG 
CCCCTGCCTC CCGAAGAGGA AGAGGAAGAG CCCACATCTC CCATCTCTCA GGAACCTGAG 
AGGAAATCAG CTGAGCTGTC AGATCAGGGA AAAACCGCAA CAGATGAAGA GAAGAAGACA 
AAGGGAAGGA AGCGCAAGAC AAAGTCCAGC TCAAGGGTTG ATGAGTATCC GCAGAGCAGC 
ATGTACCGAG TGCCTCCCAA CTACTCACCC ATCTCCTCCC AGGTGATGCA CCACCCGCAG 
TCCGCCCTGT GGGGCTACAA CCTCATGGGT CAGCCCCAGC AGCCCGGCTT CTTCCTTCAG 
AACCAGTCTG TCACTCCAGG TGGCTCCCGG TTGGACCCCG CTGGCTCCTT CGTCCCCACC 
AACACCAAGC AAGCTTTGTC CAACATGCTC CAGCGGCGCT CGGGGGCCAT GATGCAGCCG 
CCCTCTCTGC ATGCCATCAC ATCGCAGCAG CAGTTGATAC AGATGAAGCT TCTGCAGCAG 
CAGCAGCAAC AGCGGCTTCT CAGGCAAGCC CAGACGCGGC CTTTCCAACA GGGCCAGCCA 
GGGGACCAGG CTGCTCTCTT TGCTGCTCAG GCACGGCCCT CCCCTCAGCT CCCCCAGTAT 
CCAGGACTGC AGCAAGCACA GGCTGAATCA TCAGTCGCTT CAGCAGAGCC CTCTGGTGGG 
TGGAGGAATC GATGCGGTGC TGACTTCCGC ACATCCAAAC CTTCCCTCAG TGCCCTTGTC 
TCAGGACCCG ATGAGACCCA GACAGCCACA GGTTCGACAG CAGCAGAGGC TTCTCCAGAT 
GCAGCAGCCC CAGCAGCCCC CCCAGCCCCA GCAGCCTCCC CAGCCTCAGG GGTCCTCGCA 
GACCCAGAGT CAGACCCTTG GTCTCCAAAC AATGCAGCCC CAGCAGCCTC TGTTCCCCAG 
GCAAGGCTTG CAGCAGACCC AGCAGCAACA GCAGACGGCT GCCCTGGTGC GGCAGCTCCA 
GAAGCAGCTT TCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( NM_001206505.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193434.1 Bos taurus mediator complex subunit 12 like (MED12L), mRNA.

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CloneID	OBa15948
Clone ID Related Accession (Same CDS sequence)	XM_005201810.3 , NM_001206505.1
Accession Version	NM_001206505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from DAAA02002668.1, DAAA02002667.1, DAAA02002665.1, DAAA02002664.1, DAAA02002663.1 and DAAA02002656.1. On or before May 4, 2011 this sequence version replaced XM_584077.4, XM_002685015.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CCATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC GAAGTATTCA GTGCCAATGG TTCGAGCAAC ATGGCTGATC 
AAGATGACGT GTGCCTATTA CTCTGCTATT TCTGAAGCGA AAATTAAGAA ACGTCCGGCC 
ACTGATCCCA ATTTGGAGTG GACGCAAATA TCTACCAGGT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCCG ACTTCTACCA CCTGGCTTCC AGCGCGGGCG ATGGCCCTGT CCCTGTGCCA 
CCCGACGTGG AGCAGGCCAT GAGGCAGTGG GAGTACAACG AAAAGTTGGC ATTCCACATG 
TTCCAGGAAG GAATGCTGGA AAAGCATGAA TATTTGACAT GGATATTGGA TGTGTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTAT TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTCCAGTC GGCCTACCTG TCCCGTCGTC TCGCCTATTT CTGTGCCCGG 
CGTCTGGCGT TGCTGCTGAG CGACAGCCCC AGCCTCCTTG CCGCCCACTC GCCCCATGTG 
ATAATAGGCC CAAACAGCTC GAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGGC 
ATGAGCCCTG TGCAGCTGGC CTTCTCCGAC TTTCTTTCCT GTGCACAGCA CGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTTACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTACT CCACAAACGA CAATAAGAGC GCGAACCCGG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGCCT CCCCATGCCG GGAGGAAACA CGGCTTTCAA CCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCG ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AGGTATTGGA TCGACACTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGACAACCA AGAGGTTGCC 
CCCAACGATG AAGCTGTGGT GACGCTGCTG TGTGAATGGG CTGTGAGCTG TAAGCGGTCA 
GGCAAGCACA GGGCGATGGC TGTGGCGAAA CTCCTGGAGA AGAGACAAGC GGAAATCGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCCATTTC CTCAGCCTCC 
CTCGCTGGAT CTAGTTTACC TGTTTTCCAG AATGTTCTTC TAAGGTTTTT AGATACACAG 
GCCCCCTCGT TGTCGGATCC AAATAGCGAA TGTGAGAAGG TGGAGTTTGT CAACCTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGGCATGAC GTCTTCTCCC ACGATGCCTA CATGTGCACC 
CTCATCTCTC GGGGAGACCT GTCTGTCACC GCCTCCACGC GACCGCGGTC GCCAGCAGGA 
GAGAATACGG ATGAGCATTA TCCAAAGGAC CACGACATGA AAATGGAGAT TTTTTCTCCC 
ATGCCTGGAG AGTCCTGTGA GAATGCCAAC CCTTCTCTGG GCAGAAGAAT GTCAGTTAAT 
GGTGAGAAGC TGTTGAAGAG AGAAAAACCA AGGGAGTTGA TTTTTCCATC TAATTATGAC 
CTCCTACGAC ACCTGCAGTA TGCAACCCAT TTTCCCATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTTCTT TATGGAGTGG GAAAAGAGCG TGACGAAGCC 
AGACATCAGC TGAAGAAGAT TACCAAAGAT ATTTTGAAAA TCCTAAATAA GAAGAGCAAC 
ACAGAGACAG GGGTTGGAGA TGAAGGACAG AAAGCCAGGA AGAGCAAACA GGAAGCATTT 
CCGACCCTGG AGACTGTGTT CACAAAACTT CAACTCCTTT CATATTTTGA TCAGCATCAA 
GTGACATCTC AGATTTCTAA CAATGTGCTA GAACAAATCA CAAGCTTTGC ATCAGGAACA 
TCATACCATC TCCCATTGGC TCACCACATT CAGCTCATCT TTGATCTCAT GGAGCCAGCA 
CTTAATATTA ATGGACTCAT TGACTTCGCA ATACAGCTCC TGAACGAACT GAGTGTTGTG 
GAGGCAGAAC TGCTCCTGAA GTCCTCCAGC CTGGCAGGAA GCTACACGAC AGGACTGTGT 
GTCTGCATCG TGGCTGTTCT CAGGCGTTAC CACAGTTGTC TGATCCTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG ATTGTGTGGT GTGGTAAAGC ACGTTGTGAA CCCCTCAGAG 
TGCTCTTCCC CCGAAAGATG CATCTTAGCC TACCTCTACG ACCTCTACGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT AAAGCAAACC 
ATATATAACA ATGTGATTCC TGCAAATTCT AACTTGCGAT GGGATCCAGA CTTCATGATG 
GATTTTATTG AGAATCCCTC GGCCCGTAGC GTCAACTACT CAGTGCTGGG CAAGATCCTT 
AGTGACAATG CAGCCAATCG CTACAGCTTC GTCTGCAACA CTCTCATGAA TGTATGTATG 
GGTCATCAGG ATGCTGGAAG GATTAATGAC ATAGCCAATT TCTCCTCCGA GCTGACAGCC 
TGCTGCACTG TTCTCAGTTC AGAATGGCTG GGGGTCCTGA AGGCTCTTTG TTGTTCTTCA 
AATCATGTGT GGGGCTTTAA TGATGTTCTT TGCACTGTCG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT CTCCCTGGAG 
GACGTCGTGC AACACGTCGC ACTCCCCTCT CTCCTCGCCG CAGCTTGCGG AGATGCAGAT 
GCAGAGCCTG GGGCAAGAAT GACATGCCGT CTCCTGCTTC ATCTCTTTCG AGCTCCCCAG 
GCCTGCCTGT TACCTCAAGC AACTGGCAAA CCTTTCCCGG GAATAAGATC GTCTTGTGAC 
AGACACCTCT TAGCTGCTGC TCACAACAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAGTGTCAG CTCTTTAAAA 
AATGATGACT TCACCATGAG GGGTTTACGA CGTGATGGGA ATGCTGAGGA TATCTGGACT 
GCCTCACAAA ATTCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACGGC CAATTTAAGA 
GAATATGCTA GATATGTACT GAGGACCATC TGTCAACAGG AATGGGTAGG AGAACATTGT 
TTAAAAGAAC CTGAGAGATT ATGCACAGAC AAAGAACTTA TTCTGGACCC TGTGCTCTCA 
AATATGCAAG CACAGAAATT ACTGCAGCTG ATCTGTTATC CTCATGGCAT TAAGGAATGT 
ACGGAGGGGG ACAACCTCCA AAGACAGCAC ATTAAACGCA TTCTTCAGAA TCTTGAGCAG 
TGGACGCTGA GGCAGTCCTG GCTGGAGCTT CAGTTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCGGTGGC TGAAATGAAC AACCTGCTGG ACAATATTGC CAAGGCAACA 
ATAGAGGTGT TCCAGCAGTC CGCTGACCTA AACAATAACT CTTCTAATGC TGGCATGGGC 
CTCTTCAACC CAAATGGAAT TGGAAGTACT GATACAAGTA GCACGAGACA GAATGGAATA 
AAGACATTTC TAAGTTCCTC TGAACGCAGG GGTGTGTGGC TGGTGGCCCC CCTAATTGCC 
AGGCTGCCGA CTTCTGTGCA GGGAAGAGTG TTGAAAGCAG CTGGGGAAGA GCTGGAGAAA 
GGACAGCACT TGGGTTCTTC TTCCAAAAAG GAAAGAGACA GACAAAAGCA GAAAAGTATG 
TCTCTTTTGA GTCAACAACC ATTCCTCTCA CTGGTCCTTA CCTGCCTTAA GGGACAAGAT 
GAACAACGAG AAGGCCTTCT AACATCTCTG CAGAATCAAG TTAACCAGAT TTTAAGTAAC 
TGGAGAGAAG AACGATACCA GGATGACATC AAAGCACGAC AGATGATGCA TGAGGCATTG 
CAACTCCGCC TGAATTTGGT GGGAGGAATG TTTGACACAG TGCAGAGGAG CACCCAGTGG 
ACAACAGACT GGGCGCTTCT CCTCCTTCAG ATCATTACTT CTGGAACCGT TGACATGCAC 
ACCAACAATG AATTATTCAC AACAGTTCTT GACATGCTGG GTGTTTTAAT CAATGGAACA 
TTGGCCTCTG ACCTGTCGAA TGCATCCCCA GGGGGATCTG AAGAGAACAA ACGTGCATAC 
ATGAACTTAG TCAAGAAACT GAAAAAAGAG CTGGGGGACA AGCGGTCAGA AAGTATTGAC 
AAAGTTCGAC AGCTGTTACC TTTGCCGAAA CAGACATGTG ACATCATTAC CTGTGAACCT 
ATGGGCTCCT TGATTGACAC AAAGGGAAAC AAAATTGCTG GCTTCGATTC TATAGATAAA 
AAACAGGGTC TCCAGGTCTC CACGAAGCAG AAGGTGTCCC CGTGGGATTT GTTTGAGGGT 
CAGAAGAACC CGGCTCCTCT GTCCTGGGCC TGGTTCGGGA CTGTCCGAGT GGACCGGAAG 
GTGATCAAGT ATGAGGAGCA ACATCACCTC CTCCTGTATC ACACGCACCC CACGCCCAAG 
CCCCGGAGTT ACTACCTCGA GCCACTGCCC CTGCCTCCCG AAGAGGAAGA GGAAGAGCCC 
ACATCTCCCA TCTCTCAGGA ACCTGAGAGG AAATCAGCTG AGCTGTCAGA TCAGGGAAAA 
ACCGCAACAG ATGAAGAGAA GAAGACAAAG GGAAGGAAGC GCAAGACAAA GTCCAGCTCA 
AGGGTTGATG AGTATCCGCA GAGCAGCATG TACCGAGTGC CTCCCAACTA CTCACCCATC 
TCCTCCCAGG TGATGCACCA CCCGCAGTCC GCCCTGTGGG GCTACAACCT CATGGGTCAG 
CCCCAGCAGC CCGGCTTCTT CCTTCAGAAC CAGTCTGTCA CTCCAGGTGG TTCCCGGTTG 
GACCCCGCTG GCTCCTTCGT CCCCACCAAC ACCAAGCAAG CTTTGTCCAA CATGCTCCAG 
CGGCGCTCGG GGGCCATGAT GCAGCCGCCC TCTCTGCATG CCATCACATC GCAGCAGCAG 
TTGATACAGA TGAAGCTTCT GCAGCAGCAG CAGCAACAGC GGCTTCTCAG GCAAGCCCAG 
ACGCGGCCTT TCCAACAGGG CCAGCCAGGG GACCAGGCTG CTCTCTTTGC TGCTCAGGCA 
CGGCCCTCCC CTCAGCTCCC CCAGTATCCA GGACTGCAGC AAGCACAGAC CATGCCCCAG 
GGCTACACAA TGTATGGAAC CCAGATGCCT TTGCAGCAGA CCCCTCAGCA GCAGGCTGGC 
AGCGTGGTCC TGTCTCCTAC CTATAATTCC AGAACCTATC CGGCCGCTCA CTCCAACCCA 
GCGCTCATGG AAAGACTCAG ACAGATGCAG CAGCCGCCGA GCGGCTATGT TCAGCAGCAG 
GCGTCACCAT ACCTGCATCC CCTGACCGGC TCCCAGAGGC TGAATCATCA GTCGCTTCAG 
CAGAGCCCTC TGGTGGGTGG AGGAATCGAT GCGGTGCTGA CTTCCGCACA TCCAAACCTT 
CCCTCAGTGC CCTTGTCTCA GGACCCGATG AGACCCAGAC AGCCACAGGT TCGACAGCAG 
CAGAGGCTTC TCCAGATGCA GCAGCCCCAG CAGCCCCCCC AGCCCCAGCA GCCTCCCCAG 
CCTCAGGGGT CCTCGCAGAC CCAGAGTCAG ACCCTTGGTC TCCAAACAAT GCAGCCCCAG 
CAGCCTCTGT TCCCCAGGCA AGGCTTGCAG CAGACCCAGC AGCAACAGCA GACGGCTGCC 
CTGGTGCGGC AGCTCCAGAA GCAGCTTTCC AGTAACCAGC CACAGCAAGG AGTGACTCCC 
TACGGGCATC CTTCACACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193434.1
CDS	1..6444
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	NM_001206505.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206505.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CCATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC GAAGTATTCA GTGCCAATGG TTCGAGCAAC ATGGCTGATC 
AAGATGACGT GTGCCTATTA CTCTGCTATT TCTGAAGCGA AAATTAAGAA ACGTCCGGCC 
ACTGATCCCA ATTTGGAGTG GACGCAAATA TCTACCAGGT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCCG ACTTCTACCA CCTGGCTTCC AGCGCGGGCG ATGGCCCTGT CCCTGTGCCA 
CCCGACGTGG AGCAGGCCAT GAGGCAGTGG GAGTACAACG AAAAGTTGGC ATTCCACATG 
TTCCAGGAAG GAATGCTGGA AAAGCATGAA TATTTGACAT GGATATTGGA TGTGTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTAT TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTCCAGTC GGCCTACCTG TCCCGTCGTC TCGCCTATTT CTGTGCCCGG 
CGTCTGGCGT TGCTGCTGAG CGACAGCCCC AGCCTCCTTG CCGCCCACTC GCCCCATGTG 
ATAATAGGCC CAAACAGCTC GAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGGC 
ATGAGCCCTG TGCAGCTGGC CTTCTCCGAC TTTCTTTCCT GTGCACAGCA CGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTTACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTACT CCACAAACGA CAATAAGAGC GCGAACCCGG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGCCT CCCCATGCCG GGAGGAAACA CGGCTTTCAA CCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCG ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AGGTATTGGA TCGACACTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGACAACCA AGAGGTTGCC 
CCCAACGATG AAGCTGTGGT GACGCTGCTG TGTGAATGGG CTGTGAGCTG TAAGCGGTCA 
GGCAAGCACA GGGCGATGGC TGTGGCGAAA CTCCTGGAGA AGAGACAAGC GGAAATCGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCCATTTC CTCAGCCTCC 
CTCGCTGGAT CTAGTTTACC TGTTTTCCAG AATGTTCTTC TAAGGTTTTT AGATACACAG 
GCCCCCTCGT TGTCGGATCC AAATAGCGAA TGTGAGAAGG TGGAGTTTGT CAACCTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGGCATGAC GTCTTCTCCC ACGATGCCTA CATGTGCACC 
CTCATCTCTC GGGGAGACCT GTCTGTCACC GCCTCCACGC GACCGCGGTC GCCAGCAGGA 
GAGAATACGG ATGAGCATTA TCCAAAGGAC CACGACATGA AAATGGAGAT TTTTTCTCCC 
ATGCCTGGAG AGTCCTGTGA GAATGCCAAC CCTTCTCTGG GCAGAAGAAT GTCAGTTAAT 
GGTGAGAAGC TGTTGAAGAG AGAAAAACCA AGGGAGTTGA TTTTTCCATC TAATTATGAC 
CTCCTACGAC ACCTGCAGTA TGCAACCCAT TTTCCCATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTTCTT TATGGAGTGG GAAAAGAGCG TGACGAAGCC 
AGACATCAGC TGAAGAAGAT TACCAAAGAT ATTTTGAAAA TCCTAAATAA GAAGAGCAAC 
ACAGAGACAG GGGTTGGAGA TGAAGGACAG AAAGCCAGGA AGAGCAAACA GGAAGCATTT 
CCGACCCTGG AGACTGTGTT CACAAAACTT CAACTCCTTT CATATTTTGA TCAGCATCAA 
GTGACATCTC AGATTTCTAA CAATGTGCTA GAACAAATCA CAAGCTTTGC ATCAGGAACA 
TCATACCATC TCCCATTGGC TCACCACATT CAGCTCATCT TTGATCTCAT GGAGCCAGCA 
CTTAATATTA ATGGACTCAT TGACTTCGCA ATACAGCTCC TGAACGAACT GAGTGTTGTG 
GAGGCAGAAC TGCTCCTGAA GTCCTCCAGC CTGGCAGGAA GCTACACGAC AGGACTGTGT 
GTCTGCATCG TGGCTGTTCT CAGGCGTTAC CACAGTTGTC TGATCCTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG ATTGTGTGGT GTGGTAAAGC ACGTTGTGAA CCCCTCAGAG 
TGCTCTTCCC CCGAAAGATG CATCTTAGCC TACCTCTACG ACCTCTACGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT AAAGCAAACC 
ATATATAACA ATGTGATTCC TGCAAATTCT AACTTGCGAT GGGATCCAGA CTTCATGATG 
GATTTTATTG AGAATCCCTC GGCCCGTAGC GTCAACTACT CAGTGCTGGG CAAGATCCTT 
AGTGACAATG CAGCCAATCG CTACAGCTTC GTCTGCAACA CTCTCATGAA TGTATGTATG 
GGTCATCAGG ATGCTGGAAG GATTAATGAC ATAGCCAATT TCTCCTCCGA GCTGACAGCC 
TGCTGCACTG TTCTCAGTTC AGAATGGCTG GGGGTCCTGA AGGCTCTTTG TTGTTCTTCA 
AATCATGTGT GGGGCTTTAA TGATGTTCTT TGCACTGTCG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT CTCCCTGGAG 
GACGTCGTGC AACACGTCGC ACTCCCCTCT CTCCTCGCCG CAGCTTGCGG AGATGCAGAT 
GCAGAGCCTG GGGCAAGAAT GACATGCCGT CTCCTGCTTC ATCTCTTTCG AGCTCCCCAG 
GCCTGCCTGT TACCTCAAGC AACTGGCAAA CCTTTCCCGG GAATAAGATC GTCTTGTGAC 
AGACACCTCT TAGCTGCTGC TCACAACAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAGTGTCAG CTCTTTAAAA 
AATGATGACT TCACCATGAG GGGTTTACGA CGTGATGGGA ATGCTGAGGA TATCTGGACT 
GCCTCACAAA ATTCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACGGC CAATTTAAGA 
GAATATGCTA GATATGTACT GAGGACCATC TGTCAACAGG AATGGGTAGG AGAACATTGT 
TTAAAAGAAC CTGAGAGATT ATGCACAGAC AAAGAACTTA TTCTGGACCC TGTGCTCTCA 
AATATGCAAG CACAGAAATT ACTGCAGCTG ATCTGTTATC CTCATGGCAT TAAGGAATGT 
ACGGAGGGGG ACAACCTCCA AAGACAGCAC ATTAAACGCA TTCTTCAGAA TCTTGAGCAG 
TGGACGCTGA GGCAGTCCTG GCTGGAGCTT CAGTTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCGGTGGC TGAAATGAAC AACCTGCTGG ACAATATTGC CAAGGCAACA 
ATAGAGGTGT TCCAGCAGTC CGCTGACCTA AACAATAACT CTTCTAATGC TGGCATGGGC 
CTCTTCAACC CAAATGGAAT TGGAAGTACT GATACAAGTA GCACGAGACA GAATGGAATA 
AAGACATTTC TAAGTTCCTC TGAACGCAGG GGTGTGTGGC TGGTGGCCCC CCTAATTGCC 
AGGCTGCCGA CTTCTGTGCA GGGAAGAGTG TTGAAAGCAG CTGGGGAAGA GCTGGAGAAA 
GGACAGCACT TGGGTTCTTC TTCCAAAAAG GAAAGAGACA GACAAAAGCA GAAAAGTATG 
TCTCTTTTGA GTCAACAACC ATTCCTCTCA CTGGTCCTTA CCTGCCTTAA GGGACAAGAT 
GAACAACGAG AAGGCCTTCT AACATCTCTG CAGAATCAAG TTAACCAGAT TTTAAGTAAC 
TGGAGAGAAG AACGATACCA GGATGACATC AAAGCACGAC AGATGATGCA TGAGGCATTG 
CAACTCCGCC TGAATTTGGT GGGAGGAATG TTTGACACAG TGCAGAGGAG CACCCAGTGG 
ACAACAGACT GGGCGCTTCT CCTCCTTCAG ATCATTACTT CTGGAACCGT TGACATGCAC 
ACCAACAATG AATTATTCAC AACAGTTCTT GACATGCTGG GTGTTTTAAT CAATGGAACA 
TTGGCCTCTG ACCTGTCGAA TGCATCCCCA GGGGGATCTG AAGAGAACAA ACGTGCATAC 
ATGAACTTAG TCAAGAAACT GAAAAAAGAG CTGGGGGACA AGCGGTCAGA AAGTATTGAC 
AAAGTTCGAC AGCTGTTACC TTTGCCGAAA CAGACATGTG ACATCATTAC CTGTGAACCT 
ATGGGCTCCT TGATTGACAC AAAGGGAAAC AAAATTGCTG GCTTCGATTC TATAGATAAA 
AAACAGGGTC TCCAGGTCTC CACGAAGCAG AAGGTGTCCC CGTGGGATTT GTTTGAGGGT 
CAGAAGAACC CGGCTCCTCT GTCCTGGGCC TGGTTCGGGA CTGTCCGAGT GGACCGGAAG 
GTGATCAAGT ATGAGGAGCA ACATCACCTC CTCCTGTATC ACACGCACCC CACGCCCAAG 
CCCCGGAGTT ACTACCTCGA GCCACTGCCC CTGCCTCCCG AAGAGGAAGA GGAAGAGCCC 
ACATCTCCCA TCTCTCAGGA ACCTGAGAGG AAATCAGCTG AGCTGTCAGA TCAGGGAAAA 
ACCGCAACAG ATGAAGAGAA GAAGACAAAG GGAAGGAAGC GCAAGACAAA GTCCAGCTCA 
AGGGTTGATG AGTATCCGCA GAGCAGCATG TACCGAGTGC CTCCCAACTA CTCACCCATC 
TCCTCCCAGG TGATGCACCA CCCGCAGTCC GCCCTGTGGG GCTACAACCT CATGGGTCAG 
CCCCAGCAGC CCGGCTTCTT CCTTCAGAAC CAGTCTGTCA CTCCAGGTGG TTCCCGGTTG 
GACCCCGCTG GCTCCTTCGT CCCCACCAAC ACCAAGCAAG CTTTGTCCAA CATGCTCCAG 
CGGCGCTCGG GGGCCATGAT GCAGCCGCCC TCTCTGCATG CCATCACATC GCAGCAGCAG 
TTGATACAGA TGAAGCTTCT GCAGCAGCAG CAGCAACAGC GGCTTCTCAG GCAAGCCCAG 
ACGCGGCCTT TCCAACAGGG CCAGCCAGGG GACCAGGCTG CTCTCTTTGC TGCTCAGGCA 
CGGCCCTCCC CTCAGCTCCC CCAGTATCCA GGACTGCAGC AAGCACAGAC CATGCCCCAG 
GGCTACACAA TGTATGGAAC CCAGATGCCT TTGCAGCAGA CCCCTCAGCA GCAGGCTGGC 
AGCGTGGTCC TGTCTCCTAC CTATAATTCC AGAACCTATC CGGCCGCTCA CTCCAACCCA 
GCGCTCATGG AAAGACTCAG ACAGATGCAG CAGCCGCCGA GCGGCTATGT TCAGCAGCAG 
GCGTCACCAT ACCTGCATCC CCTGACCGGC TCCCAGAGGC TGAATCATCA GTCGCTTCAG 
CAGAGCCCTC TGGTGGGTGG AGGAATCGAT GCGGTGCTGA CTTCCGCACA TCCAAACCTT 
CCCTCAGTGC CCTTGTCTCA GGACCCGATG AGACCCAGAC AGCCACAGGT TCGACAGCAG 
CAGAGGCTTC TCCAGATGCA GCAGCCCCAG CAGCCCCCCC AGCCCCAGCA GCCTCCCCAG 
CCTCAGGGGT CCTCGCAGAC CCAGAGTCAG ACCCTTGGTC TCCAAACAAT GCAGCCCCAG 
CAGCCTCTGT TCCCCAGGCA AGGCTTGCAG CAGACCCAGC AGCAACAGCA GACGGCTGCC 
CTGGTGCGGC AGCTCCAGAA GCAGCTTTCC AGTAACCAGC CACAGCAAGG AGTGACTCCC 
TACGGGCATC CTTCACACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_005201808.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201865.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X5, mRNA.

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CloneID	OBa15946
Clone ID Related Accession (Same CDS sequence)	XM_005201808.3
Accession Version	XM_005201808.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103819.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201808.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAGTCCTG TGAGAATGCC 
AACCCTTCTC TGGGCAGAAG AATGTCAGTT AATGGTGAGA AGCTGTTGAA GAGAGAAAAA 
CCAAGGGAGT TGATTTTTCC ATCTAATTAT GACCTCCTAC GACACCTGCA GTATGCAACC 
CATTTTCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTT 
CTTTATGGAG TGGGAAAAGA GCGTGACGAA GCCAGACATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC AACACAGAGA CAGGGGTTGG AGATGAAGGA 
CAGAAAGCCA GGAAGAGCAA ACAGGAAGCA TTTCCGACCC TGGAGACTGT GTTCACAAAA 
CTTCAACTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATTTC TAACAATGTG 
CTAGAACAAA TCACAAGCTT TGCATCAGGA ACATCATACC ATCTCCCATT GGCTCACCAC 
ATTCAGCTCA TCTTTGATCT CATGGAGCCA GCACTTAATA TTAATGGACT CATTGACTTC 
GCAATACAGC TCCTGAACGA ACTGAGTGTT GTGGAGGCAG AACTGCTCCT GAAGTCCTCC 
AGCCTGGCAG GAAGCTACAC GACAGGACTG TGTGTCTGCA TCGTGGCTGT TCTCAGGCGT 
TACCACAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGATTGTGT 
GGTGTGGTAA AGCACGTTGT GAACCCCTCA GAGTGCTCTT CCCCCGAAAG ATGCATCTTA 
GCCTACCTCT ACGACCTCTA CGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTAGTG CCTGTTCAAA AGTAAAGCAA ACCATATATA ACAATGTGAT TCCTGCAAAT 
TCTAACTTGC GATGGGATCC AGACTTCATG ATGGATTTTA TTGAGAATCC CTCGGCCCGT 
AGCGTCAACT ACTCAGTGCT GGGCAAGATC CTTAGTGACA ATGCAGCCAA TCGCTACAGC 
TTCGTCTGCA ACACTCTCAT GAATGTATGT ATGGGTCATC AGGATGCTGG AAGGATTAAT 
GACATAGCCA ATTTCTCCTC CGAGCTGACA GCCTGCTGCA CTGTTCTCAG TTCAGAATGG 
CTGGGGGTCC TGAAGGCTCT TTGTTGTTCT TCAAATCATG TGTGGGGCTT TAATGATGTT 
CTTTGCACTG TCGATGTGAG TGACCTTTCA TTCCATGATT CATTAGCTAC TTTCATTGCT 
ATTCTGATAG CACGACAGTG TTTCTCCCTG GAGGACGTCG TGCAACACGT CGCACTCCCC 
TCTCTCCTCG CCGCAGCTTG CGGAGATGCA GATGCAGAGC CTGGGGCAAG AATGACATGC 
CGTCTCCTGC TTCATCTCTT TCGAGCTCCC CAGGCCTGCC TGTTACCTCA AGCAACTGGC 
AAACCTTTCC CGGGAATAAG ATCGTCTTGT GACAGACACC TCTTAGCTGC TGCTCACAAC 
AGCATTGAAG TGGGAGCCGT GTTTGCTGTC TTAAAAGCAA TTATGATGCT TGGAGATGCC 
AAAATTGGCA ATAACAGTGT CAGCTCTTTA AAAAATGATG ACTTCACCAT GAGGGGTTTA 
CGACGTGATG GGAATGCTGA GGATATCTGG ACTGCCTCAC AAAATTCAAA ATCCTGTGGG 
AAAAGCATTT CCATAGAAAC GGCCAATTTA AGAGAATATG CTAGATATGT ACTGAGGACC 
ATCTGTCAAC AGGAATGGGT AGGAGAACAT TGTTTAAAAG AACCTGAGAG ATTATGCACA 
GACAAAGAAC TTATTCTGGA CCCTGTGCTC TCAAATATGC AAGCACAGAA ATTACTGCAG 
CTGATCTGTT ATCCTCATGG CATTAAGGAA TGTACGGAGG GGGACAACCT CCAAAGACAG 
CACATTAAAC GCATTCTTCA GAATCTTGAG CAGTGGACGC TGAGGCAGTC CTGGCTGGAG 
CTTCAGTTAA TGATCAAACA GTGCTTGAAG GACCCTGGCT CTGGTTCGGT GGCTGAAATG 
AACAACCTGC TGGACAATAT TGCCAAGGCA ACAATAGAGG TGTTCCAGCA GTCCGCTGAC 
CTAAACAATA ACTCTTCTAA TGCTGGCATG GGCCTCTTCA ACCCAAATGG AATTGGAAGT 
ACTGATACAA GTAGCACGAG ACAGAATGGA ATAAAGACAT TTCTAAGTTC CTCTGAACGC 
AGGGGTGTGT GGCTGGTGGC CCCCCTAATT GCCAGGCTGC CGACTTCTGT GCAGGGAAGA 
GTGTTGAAAG CAGCTGGGGA AGAGCTGGAG AAAGGACAGC ACTTGGGTTC TTCTTCCAAA 
AAGGAAAGAG ACAGACAAAA GCAGAAAAGT ATGTCTCTTT TGAGTCAACA ACCATTCCTC 
TCACTGGTCC TTACCTGCCT TAAGGGACAA GATGAACAAC GAGAAGGCCT TCTAACATCT 
CTGCAGAATC AAGTTAACCA GATTTTAAGT AACTGGAGAG AAGAACGATA CCAGGATGAC 
ATCAAAGCAC GACAGATGAT GCATGAGGCA TTGCAACTCC GCCTGAATTT GGTGGGAGGA 
ATGTTTGACA CAGTGCAGAG GAGCACCCAG TGGACAACAG ACTGGGCGCT TCTCCTCCTT 
CAGATCATTA CTTCTGGAAC CGTTGACATG CACACCAACA ATGAATTATT CACAACAGTT 
CTTGACATGC TGGGTGTTTT AATCAATGGA ACATTGGCCT CTGACCTGTC GAATGCATCC 
CCAGGGGGAT CTGAAGAGAA CAAACGTGCA TACATGAACT TAGTCAAGAA ACTGAAAAAA 
GAGCTGGGGG ACAAGCGGTC AGAAAGTATT GACAAAGTTC GACAGCTGTT ACCTTTGCCG 
AAACAGACAT GTGACATCAT TACCTGTGAA CCTATGGGCT CCTTGATTGA CACAAAGGGA 
AACAAAATTG CTGGCTTCGA TTCTATAGAT AAAAAACAGG GTCTCCAGGT CTCCACGAAG 
CAGAAGGTGT CCCCGTGGGA TTTGTTTGAG GGTCAGAAGA ACCCGGCTCC TCTGTCCTGG 
GCCTGGTTCG GGACTGTCCG AGTGGACCGG AAGGTGATCA AGTATGAGGA GCAACATCAC 
CTCCTCCTGT ATCACACGCA CCCCACGCCC AAGCCCCGGA GTTACTACCT CGAGCCACTG 
CCCCTGCCTC CCGAAGAGGA AGAGGAAGAG CCCACATCTC CCATCTCTCA GGAACCTGAG 
AGGAAATCAG CTGAGCTGTC AGATCAGGGA AAAACCGCAA CAGATGAAGA GAAGAAGACA 
AAGGGAAGGA AGCGCAAGAC AAAGTCCAGC TCAAGGGTTG ATGAGTATCC GCAGAGCAGC 
ATGTACCGAG TGCCTCCCAA CTACTCACCC ATCTCCTCCC AGGTGATGCA CCACCCGCAG 
TCCGCCCTGT GGGGCTACAA CCTCATGGGT CAGCCCCAGC AGCCCGGCTT CTTCCTTCAG 
AACCAGTCTG TCACTCCAGG TGGTTCCCGG TTGGACCCCG CTGGCTCCTT CGTCCCCACC 
AACACCAAGC AAGCTTTGTC CAACATGCTC CAGCGGCGCT CGGGGGCCAT GATGCAGCCG 
CCCTCTCTGC ATGCCATCAC ATCGCAGCAG CAGTTGATAC AGATGAAGCT TCTGCAGCAG 
CAGCAGCAAC AGCGGCTTCT CAGGCAAGCC CAGACGCGGC CTTTCCAACA GGGCCAGCCA 
GGGGACCAGG CTGCTCTCTT TGCTGCTCAG GCACGGCCCT CCCCTCAGCT CCCCCAGTAT 
CCAGGACTGC AGCAAGCACA GACCATGCCC CAGGGCTACA CAATGTATGG AACCCAGATG 
CCTTTGCAGC AGACCCCTCA GCAGCAGGCT GGCAGCGTGG TCCTGTCTCC TACCTATAAT 
TCCAGAACCT ATCCGGCCGC TCACTCCAAC CCAGCGCTCA TGGAAAGACT CAGACAGATG 
CAGCAGCCGC CGAGCGGCTA TGTTCAGCAG CAGGCGTCAC CATACCTGCA TCCCCTGACC 
GGCTCCCAGA GGCTGAATCA TCAGTCGCTT CAGCAGAGCC CTCTGGTGGG TGGAGGAATC 
GATGCGGTGC TGACTTCCGC ACATCCAAAC CTTCCCTCAG TGCCCTTGTC TCAGGACCCG 
ATGAGACCCA GACAGCCACA GGTTCGACAG CAGCAGAGGC TTCTCCAGAT GCAGCAGCCC 
CAGCAGCCCC CCCAGCCCCA GCAGCCTCCC CAGCCTCAGG GGTCCTCGCA GACCCAGAGT 
CAGACCCTTG GTCTCCAAAC AATGCAGCCC CAGCAGCCTC TGTTCCCCAG GCAAGGCTTG 
CAGCAGACCC AGCAGCAACA GCAGACGGCT GCCCTGGTGC GGCAGCTCCA GAAGCAGCTT 
TCCAGTAACC AGCCACAGCA AGGAGTGACT CCCTACGGGC ATCCTTCACA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201865.1
CDS	321..6797
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_005201808.3
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201808.3

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAGTCCTG TGAGAATGCC 
AACCCTTCTC TGGGCAGAAG AATGTCAGTT AATGGTGAGA AGCTGTTGAA GAGAGAAAAA 
CCAAGGGAGT TGATTTTTCC ATCTAATTAT GACCTCCTAC GACACCTGCA GTATGCAACC 
CATTTTCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTT 
CTTTATGGAG TGGGAAAAGA GCGTGACGAA GCCAGACATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC AACACAGAGA CAGGGGTTGG AGATGAAGGA 
CAGAAAGCCA GGAAGAGCAA ACAGGAAGCA TTTCCGACCC TGGAGACTGT GTTCACAAAA 
CTTCAACTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATTTC TAACAATGTG 
CTAGAACAAA TCACAAGCTT TGCATCAGGA ACATCATACC ATCTCCCATT GGCTCACCAC 
ATTCAGCTCA TCTTTGATCT CATGGAGCCA GCACTTAATA TTAATGGACT CATTGACTTC 
GCAATACAGC TCCTGAACGA ACTGAGTGTT GTGGAGGCAG AACTGCTCCT GAAGTCCTCC 
AGCCTGGCAG GAAGCTACAC GACAGGACTG TGTGTCTGCA TCGTGGCTGT TCTCAGGCGT 
TACCACAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGATTGTGT 
GGTGTGGTAA AGCACGTTGT GAACCCCTCA GAGTGCTCTT CCCCCGAAAG ATGCATCTTA 
GCCTACCTCT ACGACCTCTA CGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTAGTG CCTGTTCAAA AGTAAAGCAA ACCATATATA ACAATGTGAT TCCTGCAAAT 
TCTAACTTGC GATGGGATCC AGACTTCATG ATGGATTTTA TTGAGAATCC CTCGGCCCGT 
AGCGTCAACT ACTCAGTGCT GGGCAAGATC CTTAGTGACA ATGCAGCCAA TCGCTACAGC 
TTCGTCTGCA ACACTCTCAT GAATGTATGT ATGGGTCATC AGGATGCTGG AAGGATTAAT 
GACATAGCCA ATTTCTCCTC CGAGCTGACA GCCTGCTGCA CTGTTCTCAG TTCAGAATGG 
CTGGGGGTCC TGAAGGCTCT TTGTTGTTCT TCAAATCATG TGTGGGGCTT TAATGATGTT 
CTTTGCACTG TCGATGTGAG TGACCTTTCA TTCCATGATT CATTAGCTAC TTTCATTGCT 
ATTCTGATAG CACGACAGTG TTTCTCCCTG GAGGACGTCG TGCAACACGT CGCACTCCCC 
TCTCTCCTCG CCGCAGCTTG CGGAGATGCA GATGCAGAGC CTGGGGCAAG AATGACATGC 
CGTCTCCTGC TTCATCTCTT TCGAGCTCCC CAGGCCTGCC TGTTACCTCA AGCAACTGGC 
AAACCTTTCC CGGGAATAAG ATCGTCTTGT GACAGACACC TCTTAGCTGC TGCTCACAAC 
AGCATTGAAG TGGGAGCCGT GTTTGCTGTC TTAAAAGCAA TTATGATGCT TGGAGATGCC 
AAAATTGGCA ATAACAGTGT CAGCTCTTTA AAAAATGATG ACTTCACCAT GAGGGGTTTA 
CGACGTGATG GGAATGCTGA GGATATCTGG ACTGCCTCAC AAAATTCAAA ATCCTGTGGG 
AAAAGCATTT CCATAGAAAC GGCCAATTTA AGAGAATATG CTAGATATGT ACTGAGGACC 
ATCTGTCAAC AGGAATGGGT AGGAGAACAT TGTTTAAAAG AACCTGAGAG ATTATGCACA 
GACAAAGAAC TTATTCTGGA CCCTGTGCTC TCAAATATGC AAGCACAGAA ATTACTGCAG 
CTGATCTGTT ATCCTCATGG CATTAAGGAA TGTACGGAGG GGGACAACCT CCAAAGACAG 
CACATTAAAC GCATTCTTCA GAATCTTGAG CAGTGGACGC TGAGGCAGTC CTGGCTGGAG 
CTTCAGTTAA TGATCAAACA GTGCTTGAAG GACCCTGGCT CTGGTTCGGT GGCTGAAATG 
AACAACCTGC TGGACAATAT TGCCAAGGCA ACAATAGAGG TGTTCCAGCA GTCCGCTGAC 
CTAAACAATA ACTCTTCTAA TGCTGGCATG GGCCTCTTCA ACCCAAATGG AATTGGAAGT 
ACTGATACAA GTAGCACGAG ACAGAATGGA ATAAAGACAT TTCTAAGTTC CTCTGAACGC 
AGGGGTGTGT GGCTGGTGGC CCCCCTAATT GCCAGGCTGC CGACTTCTGT GCAGGGAAGA 
GTGTTGAAAG CAGCTGGGGA AGAGCTGGAG AAAGGACAGC ACTTGGGTTC TTCTTCCAAA 
AAGGAAAGAG ACAGACAAAA GCAGAAAAGT ATGTCTCTTT TGAGTCAACA ACCATTCCTC 
TCACTGGTCC TTACCTGCCT TAAGGGACAA GATGAACAAC GAGAAGGCCT TCTAACATCT 
CTGCAGAATC AAGTTAACCA GATTTTAAGT AACTGGAGAG AAGAACGATA CCAGGATGAC 
ATCAAAGCAC GACAGATGAT GCATGAGGCA TTGCAACTCC GCCTGAATTT GGTGGGAGGA 
ATGTTTGACA CAGTGCAGAG GAGCACCCAG TGGACAACAG ACTGGGCGCT TCTCCTCCTT 
CAGATCATTA CTTCTGGAAC CGTTGACATG CACACCAACA ATGAATTATT CACAACAGTT 
CTTGACATGC TGGGTGTTTT AATCAATGGA ACATTGGCCT CTGACCTGTC GAATGCATCC 
CCAGGGGGAT CTGAAGAGAA CAAACGTGCA TACATGAACT TAGTCAAGAA ACTGAAAAAA 
GAGCTGGGGG ACAAGCGGTC AGAAAGTATT GACAAAGTTC GACAGCTGTT ACCTTTGCCG 
AAACAGACAT GTGACATCAT TACCTGTGAA CCTATGGGCT CCTTGATTGA CACAAAGGGA 
AACAAAATTG CTGGCTTCGA TTCTATAGAT AAAAAACAGG GTCTCCAGGT CTCCACGAAG 
CAGAAGGTGT CCCCGTGGGA TTTGTTTGAG GGTCAGAAGA ACCCGGCTCC TCTGTCCTGG 
GCCTGGTTCG GGACTGTCCG AGTGGACCGG AAGGTGATCA AGTATGAGGA GCAACATCAC 
CTCCTCCTGT ATCACACGCA CCCCACGCCC AAGCCCCGGA GTTACTACCT CGAGCCACTG 
CCCCTGCCTC CCGAAGAGGA AGAGGAAGAG CCCACATCTC CCATCTCTCA GGAACCTGAG 
AGGAAATCAG CTGAGCTGTC AGATCAGGGA AAAACCGCAA CAGATGAAGA GAAGAAGACA 
AAGGGAAGGA AGCGCAAGAC AAAGTCCAGC TCAAGGGTTG ATGAGTATCC GCAGAGCAGC 
ATGTACCGAG TGCCTCCCAA CTACTCACCC ATCTCCTCCC AGGTGATGCA CCACCCGCAG 
TCCGCCCTGT GGGGCTACAA CCTCATGGGT CAGCCCCAGC AGCCCGGCTT CTTCCTTCAG 
AACCAGTCTG TCACTCCAGG TGGTTCCCGG TTGGACCCCG CTGGCTCCTT CGTCCCCACC 
AACACCAAGC AAGCTTTGTC CAACATGCTC CAGCGGCGCT CGGGGGCCAT GATGCAGCCG 
CCCTCTCTGC ATGCCATCAC ATCGCAGCAG CAGTTGATAC AGATGAAGCT TCTGCAGCAG 
CAGCAGCAAC AGCGGCTTCT CAGGCAAGCC CAGACGCGGC CTTTCCAACA GGGCCAGCCA 
GGGGACCAGG CTGCTCTCTT TGCTGCTCAG GCACGGCCCT CCCCTCAGCT CCCCCAGTAT 
CCAGGACTGC AGCAAGCACA GACCATGCCC CAGGGCTACA CAATGTATGG AACCCAGATG 
CCTTTGCAGC AGACCCCTCA GCAGCAGGCT GGCAGCGTGG TCCTGTCTCC TACCTATAAT 
TCCAGAACCT ATCCGGCCGC TCACTCCAAC CCAGCGCTCA TGGAAAGACT CAGACAGATG 
CAGCAGCCGC CGAGCGGCTA TGTTCAGCAG CAGGCGTCAC CATACCTGCA TCCCCTGACC 
GGCTCCCAGA GGCTGAATCA TCAGTCGCTT CAGCAGAGCC CTCTGGTGGG TGGAGGAATC 
GATGCGGTGC TGACTTCCGC ACATCCAAAC CTTCCCTCAG TGCCCTTGTC TCAGGACCCG 
ATGAGACCCA GACAGCCACA GGTTCGACAG CAGCAGAGGC TTCTCCAGAT GCAGCAGCCC 
CAGCAGCCCC CCCAGCCCCA GCAGCCTCCC CAGCCTCAGG GGTCCTCGCA GACCCAGAGT 
CAGACCCTTG GTCTCCAAAC AATGCAGCCC CAGCAGCCTC TGTTCCCCAG GCAAGGCTTG 
CAGCAGACCC AGCAGCAACA GCAGACGGCT GCCCTGGTGC GGCAGCTCCA GAAGCAGCTT 
TCCAGTAACC AGCCACAGCA AGGAGTGACT CCCTACGGGC ATCCTTCACA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_005201810.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201867.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X7, mRNA.

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CloneID	OBa15948
Clone ID Related Accession (Same CDS sequence)	XM_005201810.3 , NM_001206505.1
Accession Version	XM_005201810.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103819.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201810.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CCATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC GAAGTATTCA GTGCCAATGG TTCGAGCAAC ATGGCTGATC 
AAGATGACGT GTGCCTATTA CTCTGCTATT TCTGAAGCGA AAATTAAGAA ACGTCCGGCC 
ACTGATCCCA ATTTGGAGTG GACGCAAATA TCTACCAGGT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCCG ACTTCTACCA CCTGGCTTCC AGCGCGGGCG ATGGCCCTGT CCCTGTGCCA 
CCCGACGTGG AGCAGGCCAT GAGGCAGTGG GAGTACAACG AAAAGTTGGC ATTCCACATG 
TTCCAGGAAG GAATGCTGGA AAAGCATGAA TATTTGACAT GGATATTGGA TGTGTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTAT TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTCCAGTC GGCCTACCTG TCCCGTCGTC TCGCCTATTT CTGTGCCCGG 
CGTCTGGCGT TGCTGCTGAG CGACAGCCCC AGCCTCCTTG CCGCCCACTC GCCCCATGTG 
ATAATAGGCC CAAACAGCTC GAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGGC 
ATGAGCCCTG TGCAGCTGGC CTTCTCCGAC TTTCTTTCCT GTGCACAGCA CGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTTACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTACT CCACAAACGA CAATAAGAGC GCGAACCCGG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGCCT CCCCATGCCG GGAGGAAACA CGGCTTTCAA CCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCG ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AGGTATTGGA TCGACACTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGACAACCA AGAGGTTGCC 
CCCAACGATG AAGCTGTGGT GACGCTGCTG TGTGAATGGG CTGTGAGCTG TAAGCGGTCA 
GGCAAGCACA GGGCGATGGC TGTGGCGAAA CTCCTGGAGA AGAGACAAGC GGAAATCGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCCATTTC CTCAGCCTCC 
CTCGCTGGAT CTAGTTTACC TGTTTTCCAG AATGTTCTTC TAAGGTTTTT AGATACACAG 
GCCCCCTCGT TGTCGGATCC AAATAGCGAA TGTGAGAAGG TGGAGTTTGT CAACCTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGGCATGAC GTCTTCTCCC ACGATGCCTA CATGTGCACC 
CTCATCTCTC GGGGAGACCT GTCTGTCACC GCCTCCACGC GACCGCGGTC GCCAGCAGGA 
GAGAATACGG ATGAGCATTA TCCAAAGGAC CACGACATGA AAATGGAGAT TTTTTCTCCC 
ATGCCTGGAG AGTCCTGTGA GAATGCCAAC CCTTCTCTGG GCAGAAGAAT GTCAGTTAAT 
GGTGAGAAGC TGTTGAAGAG AGAAAAACCA AGGGAGTTGA TTTTTCCATC TAATTATGAC 
CTCCTACGAC ACCTGCAGTA TGCAACCCAT TTTCCCATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTTCTT TATGGAGTGG GAAAAGAGCG TGACGAAGCC 
AGACATCAGC TGAAGAAGAT TACCAAAGAT ATTTTGAAAA TCCTAAATAA GAAGAGCAAC 
ACAGAGACAG GGGTTGGAGA TGAAGGACAG AAAGCCAGGA AGAGCAAACA GGAAGCATTT 
CCGACCCTGG AGACTGTGTT CACAAAACTT CAACTCCTTT CATATTTTGA TCAGCATCAA 
GTGACATCTC AGATTTCTAA CAATGTGCTA GAACAAATCA CAAGCTTTGC ATCAGGAACA 
TCATACCATC TCCCATTGGC TCACCACATT CAGCTCATCT TTGATCTCAT GGAGCCAGCA 
CTTAATATTA ATGGACTCAT TGACTTCGCA ATACAGCTCC TGAACGAACT GAGTGTTGTG 
GAGGCAGAAC TGCTCCTGAA GTCCTCCAGC CTGGCAGGAA GCTACACGAC AGGACTGTGT 
GTCTGCATCG TGGCTGTTCT CAGGCGTTAC CACAGTTGTC TGATCCTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG ATTGTGTGGT GTGGTAAAGC ACGTTGTGAA CCCCTCAGAG 
TGCTCTTCCC CCGAAAGATG CATCTTAGCC TACCTCTACG ACCTCTACGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT AAAGCAAACC 
ATATATAACA ATGTGATTCC TGCAAATTCT AACTTGCGAT GGGATCCAGA CTTCATGATG 
GATTTTATTG AGAATCCCTC GGCCCGTAGC GTCAACTACT CAGTGCTGGG CAAGATCCTT 
AGTGACAATG CAGCCAATCG CTACAGCTTC GTCTGCAACA CTCTCATGAA TGTATGTATG 
GGTCATCAGG ATGCTGGAAG GATTAATGAC ATAGCCAATT TCTCCTCCGA GCTGACAGCC 
TGCTGCACTG TTCTCAGTTC AGAATGGCTG GGGGTCCTGA AGGCTCTTTG TTGTTCTTCA 
AATCATGTGT GGGGCTTTAA TGATGTTCTT TGCACTGTCG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT CTCCCTGGAG 
GACGTCGTGC AACACGTCGC ACTCCCCTCT CTCCTCGCCG CAGCTTGCGG AGATGCAGAT 
GCAGAGCCTG GGGCAAGAAT GACATGCCGT CTCCTGCTTC ATCTCTTTCG AGCTCCCCAG 
GCCTGCCTGT TACCTCAAGC AACTGGCAAA CCTTTCCCGG GAATAAGATC GTCTTGTGAC 
AGACACCTCT TAGCTGCTGC TCACAACAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAGTGTCAG CTCTTTAAAA 
AATGATGACT TCACCATGAG GGGTTTACGA CGTGATGGGA ATGCTGAGGA TATCTGGACT 
GCCTCACAAA ATTCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACGGC CAATTTAAGA 
GAATATGCTA GATATGTACT GAGGACCATC TGTCAACAGG AATGGGTAGG AGAACATTGT 
TTAAAAGAAC CTGAGAGATT ATGCACAGAC AAAGAACTTA TTCTGGACCC TGTGCTCTCA 
AATATGCAAG CACAGAAATT ACTGCAGCTG ATCTGTTATC CTCATGGCAT TAAGGAATGT 
ACGGAGGGGG ACAACCTCCA AAGACAGCAC ATTAAACGCA TTCTTCAGAA TCTTGAGCAG 
TGGACGCTGA GGCAGTCCTG GCTGGAGCTT CAGTTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCGGTGGC TGAAATGAAC AACCTGCTGG ACAATATTGC CAAGGCAACA 
ATAGAGGTGT TCCAGCAGTC CGCTGACCTA AACAATAACT CTTCTAATGC TGGCATGGGC 
CTCTTCAACC CAAATGGAAT TGGAAGTACT GATACAAGTA GCACGAGACA GAATGGAATA 
AAGACATTTC TAAGTTCCTC TGAACGCAGG GGTGTGTGGC TGGTGGCCCC CCTAATTGCC 
AGGCTGCCGA CTTCTGTGCA GGGAAGAGTG TTGAAAGCAG CTGGGGAAGA GCTGGAGAAA 
GGACAGCACT TGGGTTCTTC TTCCAAAAAG GAAAGAGACA GACAAAAGCA GAAAAGTATG 
TCTCTTTTGA GTCAACAACC ATTCCTCTCA CTGGTCCTTA CCTGCCTTAA GGGACAAGAT 
GAACAACGAG AAGGCCTTCT AACATCTCTG CAGAATCAAG TTAACCAGAT TTTAAGTAAC 
TGGAGAGAAG AACGATACCA GGATGACATC AAAGCACGAC AGATGATGCA TGAGGCATTG 
CAACTCCGCC TGAATTTGGT GGGAGGAATG TTTGACACAG TGCAGAGGAG CACCCAGTGG 
ACAACAGACT GGGCGCTTCT CCTCCTTCAG ATCATTACTT CTGGAACCGT TGACATGCAC 
ACCAACAATG AATTATTCAC AACAGTTCTT GACATGCTGG GTGTTTTAAT CAATGGAACA 
TTGGCCTCTG ACCTGTCGAA TGCATCCCCA GGGGGATCTG AAGAGAACAA ACGTGCATAC 
ATGAACTTAG TCAAGAAACT GAAAAAAGAG CTGGGGGACA AGCGGTCAGA AAGTATTGAC 
AAAGTTCGAC AGCTGTTACC TTTGCCGAAA CAGACATGTG ACATCATTAC CTGTGAACCT 
ATGGGCTCCT TGATTGACAC AAAGGGAAAC AAAATTGCTG GCTTCGATTC TATAGATAAA 
AAACAGGGTC TCCAGGTCTC CACGAAGCAG AAGGTGTCCC CGTGGGATTT GTTTGAGGGT 
CAGAAGAACC CGGCTCCTCT GTCCTGGGCC TGGTTCGGGA CTGTCCGAGT GGACCGGAAG 
GTGATCAAGT ATGAGGAGCA ACATCACCTC CTCCTGTATC ACACGCACCC CACGCCCAAG 
CCCCGGAGTT ACTACCTCGA GCCACTGCCC CTGCCTCCCG AAGAGGAAGA GGAAGAGCCC 
ACATCTCCCA TCTCTCAGGA ACCTGAGAGG AAATCAGCTG AGCTGTCAGA TCAGGGAAAA 
ACCGCAACAG ATGAAGAGAA GAAGACAAAG GGAAGGAAGC GCAAGACAAA GTCCAGCTCA 
AGGGTTGATG AGTATCCGCA GAGCAGCATG TACCGAGTGC CTCCCAACTA CTCACCCATC 
TCCTCCCAGG TGATGCACCA CCCGCAGTCC GCCCTGTGGG GCTACAACCT CATGGGTCAG 
CCCCAGCAGC CCGGCTTCTT CCTTCAGAAC CAGTCTGTCA CTCCAGGTGG TTCCCGGTTG 
GACCCCGCTG GCTCCTTCGT CCCCACCAAC ACCAAGCAAG CTTTGTCCAA CATGCTCCAG 
CGGCGCTCGG GGGCCATGAT GCAGCCGCCC TCTCTGCATG CCATCACATC GCAGCAGCAG 
TTGATACAGA TGAAGCTTCT GCAGCAGCAG CAGCAACAGC GGCTTCTCAG GCAAGCCCAG 
ACGCGGCCTT TCCAACAGGG CCAGCCAGGG GACCAGGCTG CTCTCTTTGC TGCTCAGGCA 
CGGCCCTCCC CTCAGCTCCC CCAGTATCCA GGACTGCAGC AAGCACAGAC CATGCCCCAG 
GGCTACACAA TGTATGGAAC CCAGATGCCT TTGCAGCAGA CCCCTCAGCA GCAGGCTGGC 
AGCGTGGTCC TGTCTCCTAC CTATAATTCC AGAACCTATC CGGCCGCTCA CTCCAACCCA 
GCGCTCATGG AAAGACTCAG ACAGATGCAG CAGCCGCCGA GCGGCTATGT TCAGCAGCAG 
GCGTCACCAT ACCTGCATCC CCTGACCGGC TCCCAGAGGC TGAATCATCA GTCGCTTCAG 
CAGAGCCCTC TGGTGGGTGG AGGAATCGAT GCGGTGCTGA CTTCCGCACA TCCAAACCTT 
CCCTCAGTGC CCTTGTCTCA GGACCCGATG AGACCCAGAC AGCCACAGGT TCGACAGCAG 
CAGAGGCTTC TCCAGATGCA GCAGCCCCAG CAGCCCCCCC AGCCCCAGCA GCCTCCCCAG 
CCTCAGGGGT CCTCGCAGAC CCAGAGTCAG ACCCTTGGTC TCCAAACAAT GCAGCCCCAG 
CAGCCTCTGT TCCCCAGGCA AGGCTTGCAG CAGACCCAGC AGCAACAGCA GACGGCTGCC 
CTGGTGCGGC AGCTCCAGAA GCAGCTTTCC AGTAACCAGC CACAGCAAGG AGTGACTCCC 
TACGGGCATC CTTCACACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201867.1
CDS	321..6764
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_005201810.3
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201810.3

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CCATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC GAAGTATTCA GTGCCAATGG TTCGAGCAAC ATGGCTGATC 
AAGATGACGT GTGCCTATTA CTCTGCTATT TCTGAAGCGA AAATTAAGAA ACGTCCGGCC 
ACTGATCCCA ATTTGGAGTG GACGCAAATA TCTACCAGGT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCCG ACTTCTACCA CCTGGCTTCC AGCGCGGGCG ATGGCCCTGT CCCTGTGCCA 
CCCGACGTGG AGCAGGCCAT GAGGCAGTGG GAGTACAACG AAAAGTTGGC ATTCCACATG 
TTCCAGGAAG GAATGCTGGA AAAGCATGAA TATTTGACAT GGATATTGGA TGTGTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTAT TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTCCAGTC GGCCTACCTG TCCCGTCGTC TCGCCTATTT CTGTGCCCGG 
CGTCTGGCGT TGCTGCTGAG CGACAGCCCC AGCCTCCTTG CCGCCCACTC GCCCCATGTG 
ATAATAGGCC CAAACAGCTC GAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGGC 
ATGAGCCCTG TGCAGCTGGC CTTCTCCGAC TTTCTTTCCT GTGCACAGCA CGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTTACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTACT CCACAAACGA CAATAAGAGC GCGAACCCGG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGCCT CCCCATGCCG GGAGGAAACA CGGCTTTCAA CCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCG ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AGGTATTGGA TCGACACTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGACAACCA AGAGGTTGCC 
CCCAACGATG AAGCTGTGGT GACGCTGCTG TGTGAATGGG CTGTGAGCTG TAAGCGGTCA 
GGCAAGCACA GGGCGATGGC TGTGGCGAAA CTCCTGGAGA AGAGACAAGC GGAAATCGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCCATTTC CTCAGCCTCC 
CTCGCTGGAT CTAGTTTACC TGTTTTCCAG AATGTTCTTC TAAGGTTTTT AGATACACAG 
GCCCCCTCGT TGTCGGATCC AAATAGCGAA TGTGAGAAGG TGGAGTTTGT CAACCTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGGCATGAC GTCTTCTCCC ACGATGCCTA CATGTGCACC 
CTCATCTCTC GGGGAGACCT GTCTGTCACC GCCTCCACGC GACCGCGGTC GCCAGCAGGA 
GAGAATACGG ATGAGCATTA TCCAAAGGAC CACGACATGA AAATGGAGAT TTTTTCTCCC 
ATGCCTGGAG AGTCCTGTGA GAATGCCAAC CCTTCTCTGG GCAGAAGAAT GTCAGTTAAT 
GGTGAGAAGC TGTTGAAGAG AGAAAAACCA AGGGAGTTGA TTTTTCCATC TAATTATGAC 
CTCCTACGAC ACCTGCAGTA TGCAACCCAT TTTCCCATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTTCTT TATGGAGTGG GAAAAGAGCG TGACGAAGCC 
AGACATCAGC TGAAGAAGAT TACCAAAGAT ATTTTGAAAA TCCTAAATAA GAAGAGCAAC 
ACAGAGACAG GGGTTGGAGA TGAAGGACAG AAAGCCAGGA AGAGCAAACA GGAAGCATTT 
CCGACCCTGG AGACTGTGTT CACAAAACTT CAACTCCTTT CATATTTTGA TCAGCATCAA 
GTGACATCTC AGATTTCTAA CAATGTGCTA GAACAAATCA CAAGCTTTGC ATCAGGAACA 
TCATACCATC TCCCATTGGC TCACCACATT CAGCTCATCT TTGATCTCAT GGAGCCAGCA 
CTTAATATTA ATGGACTCAT TGACTTCGCA ATACAGCTCC TGAACGAACT GAGTGTTGTG 
GAGGCAGAAC TGCTCCTGAA GTCCTCCAGC CTGGCAGGAA GCTACACGAC AGGACTGTGT 
GTCTGCATCG TGGCTGTTCT CAGGCGTTAC CACAGTTGTC TGATCCTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG ATTGTGTGGT GTGGTAAAGC ACGTTGTGAA CCCCTCAGAG 
TGCTCTTCCC CCGAAAGATG CATCTTAGCC TACCTCTACG ACCTCTACGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT AAAGCAAACC 
ATATATAACA ATGTGATTCC TGCAAATTCT AACTTGCGAT GGGATCCAGA CTTCATGATG 
GATTTTATTG AGAATCCCTC GGCCCGTAGC GTCAACTACT CAGTGCTGGG CAAGATCCTT 
AGTGACAATG CAGCCAATCG CTACAGCTTC GTCTGCAACA CTCTCATGAA TGTATGTATG 
GGTCATCAGG ATGCTGGAAG GATTAATGAC ATAGCCAATT TCTCCTCCGA GCTGACAGCC 
TGCTGCACTG TTCTCAGTTC AGAATGGCTG GGGGTCCTGA AGGCTCTTTG TTGTTCTTCA 
AATCATGTGT GGGGCTTTAA TGATGTTCTT TGCACTGTCG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT CTCCCTGGAG 
GACGTCGTGC AACACGTCGC ACTCCCCTCT CTCCTCGCCG CAGCTTGCGG AGATGCAGAT 
GCAGAGCCTG GGGCAAGAAT GACATGCCGT CTCCTGCTTC ATCTCTTTCG AGCTCCCCAG 
GCCTGCCTGT TACCTCAAGC AACTGGCAAA CCTTTCCCGG GAATAAGATC GTCTTGTGAC 
AGACACCTCT TAGCTGCTGC TCACAACAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAGTGTCAG CTCTTTAAAA 
AATGATGACT TCACCATGAG GGGTTTACGA CGTGATGGGA ATGCTGAGGA TATCTGGACT 
GCCTCACAAA ATTCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACGGC CAATTTAAGA 
GAATATGCTA GATATGTACT GAGGACCATC TGTCAACAGG AATGGGTAGG AGAACATTGT 
TTAAAAGAAC CTGAGAGATT ATGCACAGAC AAAGAACTTA TTCTGGACCC TGTGCTCTCA 
AATATGCAAG CACAGAAATT ACTGCAGCTG ATCTGTTATC CTCATGGCAT TAAGGAATGT 
ACGGAGGGGG ACAACCTCCA AAGACAGCAC ATTAAACGCA TTCTTCAGAA TCTTGAGCAG 
TGGACGCTGA GGCAGTCCTG GCTGGAGCTT CAGTTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCGGTGGC TGAAATGAAC AACCTGCTGG ACAATATTGC CAAGGCAACA 
ATAGAGGTGT TCCAGCAGTC CGCTGACCTA AACAATAACT CTTCTAATGC TGGCATGGGC 
CTCTTCAACC CAAATGGAAT TGGAAGTACT GATACAAGTA GCACGAGACA GAATGGAATA 
AAGACATTTC TAAGTTCCTC TGAACGCAGG GGTGTGTGGC TGGTGGCCCC CCTAATTGCC 
AGGCTGCCGA CTTCTGTGCA GGGAAGAGTG TTGAAAGCAG CTGGGGAAGA GCTGGAGAAA 
GGACAGCACT TGGGTTCTTC TTCCAAAAAG GAAAGAGACA GACAAAAGCA GAAAAGTATG 
TCTCTTTTGA GTCAACAACC ATTCCTCTCA CTGGTCCTTA CCTGCCTTAA GGGACAAGAT 
GAACAACGAG AAGGCCTTCT AACATCTCTG CAGAATCAAG TTAACCAGAT TTTAAGTAAC 
TGGAGAGAAG AACGATACCA GGATGACATC AAAGCACGAC AGATGATGCA TGAGGCATTG 
CAACTCCGCC TGAATTTGGT GGGAGGAATG TTTGACACAG TGCAGAGGAG CACCCAGTGG 
ACAACAGACT GGGCGCTTCT CCTCCTTCAG ATCATTACTT CTGGAACCGT TGACATGCAC 
ACCAACAATG AATTATTCAC AACAGTTCTT GACATGCTGG GTGTTTTAAT CAATGGAACA 
TTGGCCTCTG ACCTGTCGAA TGCATCCCCA GGGGGATCTG AAGAGAACAA ACGTGCATAC 
ATGAACTTAG TCAAGAAACT GAAAAAAGAG CTGGGGGACA AGCGGTCAGA AAGTATTGAC 
AAAGTTCGAC AGCTGTTACC TTTGCCGAAA CAGACATGTG ACATCATTAC CTGTGAACCT 
ATGGGCTCCT TGATTGACAC AAAGGGAAAC AAAATTGCTG GCTTCGATTC TATAGATAAA 
AAACAGGGTC TCCAGGTCTC CACGAAGCAG AAGGTGTCCC CGTGGGATTT GTTTGAGGGT 
CAGAAGAACC CGGCTCCTCT GTCCTGGGCC TGGTTCGGGA CTGTCCGAGT GGACCGGAAG 
GTGATCAAGT ATGAGGAGCA ACATCACCTC CTCCTGTATC ACACGCACCC CACGCCCAAG 
CCCCGGAGTT ACTACCTCGA GCCACTGCCC CTGCCTCCCG AAGAGGAAGA GGAAGAGCCC 
ACATCTCCCA TCTCTCAGGA ACCTGAGAGG AAATCAGCTG AGCTGTCAGA TCAGGGAAAA 
ACCGCAACAG ATGAAGAGAA GAAGACAAAG GGAAGGAAGC GCAAGACAAA GTCCAGCTCA 
AGGGTTGATG AGTATCCGCA GAGCAGCATG TACCGAGTGC CTCCCAACTA CTCACCCATC 
TCCTCCCAGG TGATGCACCA CCCGCAGTCC GCCCTGTGGG GCTACAACCT CATGGGTCAG 
CCCCAGCAGC CCGGCTTCTT CCTTCAGAAC CAGTCTGTCA CTCCAGGTGG TTCCCGGTTG 
GACCCCGCTG GCTCCTTCGT CCCCACCAAC ACCAAGCAAG CTTTGTCCAA CATGCTCCAG 
CGGCGCTCGG GGGCCATGAT GCAGCCGCCC TCTCTGCATG CCATCACATC GCAGCAGCAG 
TTGATACAGA TGAAGCTTCT GCAGCAGCAG CAGCAACAGC GGCTTCTCAG GCAAGCCCAG 
ACGCGGCCTT TCCAACAGGG CCAGCCAGGG GACCAGGCTG CTCTCTTTGC TGCTCAGGCA 
CGGCCCTCCC CTCAGCTCCC CCAGTATCCA GGACTGCAGC AAGCACAGAC CATGCCCCAG 
GGCTACACAA TGTATGGAAC CCAGATGCCT TTGCAGCAGA CCCCTCAGCA GCAGGCTGGC 
AGCGTGGTCC TGTCTCCTAC CTATAATTCC AGAACCTATC CGGCCGCTCA CTCCAACCCA 
GCGCTCATGG AAAGACTCAG ACAGATGCAG CAGCCGCCGA GCGGCTATGT TCAGCAGCAG 
GCGTCACCAT ACCTGCATCC CCTGACCGGC TCCCAGAGGC TGAATCATCA GTCGCTTCAG 
CAGAGCCCTC TGGTGGGTGG AGGAATCGAT GCGGTGCTGA CTTCCGCACA TCCAAACCTT 
CCCTCAGTGC CCTTGTCTCA GGACCCGATG AGACCCAGAC AGCCACAGGT TCGACAGCAG 
CAGAGGCTTC TCCAGATGCA GCAGCCCCAG CAGCCCCCCC AGCCCCAGCA GCCTCCCCAG 
CCTCAGGGGT CCTCGCAGAC CCAGAGTCAG ACCCTTGGTC TCCAAACAAT GCAGCCCCAG 
CAGCCTCTGT TCCCCAGGCA AGGCTTGCAG CAGACCCAGC AGCAACAGCA GACGGCTGCC 
CTGGTGCGGC AGCTCCAGAA GCAGCTTTCC AGTAACCAGC CACAGCAAGG AGTGACTCCC 
TACGGGCATC CTTCACACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_010801486.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799788.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X2, mRNA.

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CloneID	OBa54150
Clone ID Related Accession (Same CDS sequence)	XM_010801486.2 , XM_010801485.2
Accession Version	XM_010801486.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103819.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GGAACAGACT ATTATGGCAC ATATGGGCAT TGACTCAGGA 
ACTACTAATA TTTTTGATGA AGTAGACAAG AATGACTTTA AAACTGACTT TGGTTCGGAA 
TTTCCAATTT TTTCTCCCAT GCCTGGAGAG TCCTGTGAGA ATGCCAACCC TTCTCTGGGC 
AGAAGAATGT CAGTTAATGG TGAGAAGCTG TTGAAGAGAG AAAAACCAAG GGAGTTGATT 
TTTCCATCTA ATTATGACCT CCTACGACAC CTGCAGTATG CAACCCATTT TCCCATACCT 
CTGGATGAAT CTTCAAGTCA TGAATGTAAC CAGCGCACAA TCCTTCTTTA TGGAGTGGGA 
AAAGAGCGTG ACGAAGCCAG ACATCAGCTG AAGAAGATTA CCAAAGATAT TTTGAAAATC 
CTAAATAAGA AGAGCAACAC AGAGACAGGG GTTGGAGATG AAGGACAGAA AGCCAGGAAG 
AGCAAACAGG AAGCATTTCC GACCCTGGAG ACTGTGTTCA CAAAACTTCA ACTCCTTTCA 
TATTTTGATC AGCATCAAGT GACATCTCAG ATTTCTAACA ATGTGCTAGA ACAAATCACA 
AGCTTTGCAT CAGGAACATC ATACCATCTC CCATTGGCTC ACCACATTCA GCTCATCTTT 
GATCTCATGG AGCCAGCACT TAATATTAAT GGACTCATTG ACTTCGCAAT ACAGCTCCTG 
AACGAACTGA GTGTTGTGGA GGCAGAACTG CTCCTGAAGT CCTCCAGCCT GGCAGGAAGC 
TACACGACAG GACTGTGTGT CTGCATCGTG GCTGTTCTCA GGCGTTACCA CAGTTGTCTG 
ATCCTGAATC CTGATCAGAC AGCCCAGGTG TTTGAAGGAT TGTGTGGTGT GGTAAAGCAC 
GTTGTGAACC CCTCAGAGTG CTCTTCCCCC GAAAGATGCA TCTTAGCCTA CCTCTACGAC 
CTCTACGTGT CATGTAGCCA CCTCAGAAGT AAATTTGGAG ACCTCTTCAG TAGTGCCTGT 
TCAAAAGTAA AGCAAACCAT ATATAACAAT GTGATTCCTG CAAATTCTAA CTTGCGATGG 
GATCCAGACT TCATGATGGA TTTTATTGAG AATCCCTCGG CCCGTAGCGT CAACTACTCA 
GTGCTGGGCA AGATCCTTAG TGACAATGCA GCCAATCGCT ACAGCTTCGT CTGCAACACT 
CTCATGAATG TATGTATGGG TCATCAGGAT GCTGGAAGGA TTAATGACAT AGCCAATTTC 
TCCTCCGAGC TGACAGCCTG CTGCACTGTT CTCAGTTCAG AATGGCTGGG GGTCCTGAAG 
GCTCTTTGTT GTTCTTCAAA TCATGTGTGG GGCTTTAATG ATGTTCTTTG CACTGTCGAT 
GTGAGTGACC TTTCATTCCA TGATTCATTA GCTACTTTCA TTGCTATTCT GATAGCACGA 
CAGTGTTTCT CCCTGGAGGA CGTCGTGCAA CACGTCGCAC TCCCCTCTCT CCTCGCCGCA 
GCTTGCGGAG ATGCAGATGC AGAGCCTGGG GCAAGAATGA CATGCCGTCT CCTGCTTCAT 
CTCTTTCGAG CTCCCCAGGC CTGCCTGTTA CCTCAAGCAA CTGGCAAACC TTTCCCGGGA 
ATAAGATCGT CTTGTGACAG ACACCTCTTA GCTGCTGCTC ACAACAGCAT TGAAGTGGGA 
GCCGTGTTTG CTGTCTTAAA AGCAATTATG ATGCTTGGAG ATGCCAAAAT TGGCAATAAC 
AGTGTCAGCT CTTTAAAAAA TGATGACTTC ACCATGAGGG GTTTACGACG TGATGGGAAT 
GCTGAGGATA TCTGGACTGC CTCACAAAAT TCAAAATCCT GTGGGAAAAG CATTTCCATA 
GAAACGGCCA ATTTAAGAGA ATATGCTAGA TATGTACTGA GGACCATCTG TCAACAGGAA 
TGGGTAGGAG AACATTGTTT AAAAGAACCT GAGAGATTAT GCACAGACAA AGAACTTATT 
CTGGACCCTG TGCTCTCAAA TATGCAAGCA CAGAAATTAC TGCAGCTGAT CTGTTATCCT 
CATGGCATTA AGGAATGTAC GGAGGGGGAC AACCTCCAAA GACAGCACAT TAAACGCATT 
CTTCAGAATC TTGAGCAGTG GACGCTGAGG CAGTCCTGGC TGGAGCTTCA GTTAATGATC 
AAACAGTGCT TGAAGGACCC TGGCTCTGGT TCGGTGGCTG AAATGAACAA CCTGCTGGAC 
AATATTGCCA AGGCAACAAT AGAGGTGTTC CAGCAGTCCG CTGACCTAAA CAATAACTCT 
TCTAATGCTG GCATGGGCCT CTTCAACCCA AATGGAATTG GAAGTACTGA TACAAGTAGC 
ACGAGACAGA ATGGAATAAA GACATTTCTA AGTTCCTCTG AACGCAGGGG TGTGTGGCTG 
GTGGCCCCCC TAATTGCCAG GCTGCCGACT TCTGTGCAGG GAAGAGTGTT GAAAGCAGCT 
GGGGAAGAGC TGGAGAAAGG ACAGCACTTG GGTTCTTCTT CCAAAAAGGA AAGAGACAGA 
CAAAAGCAGA AAAGTATGTC TCTTTTGAGT CAACAACCAT TCCTCTCACT GGTCCTTACC 
TGCCTTAAGG GACAAGATGA ACAACGAGAA GGCCTTCTAA CATCTCTGCA GAATCAAGTT 
AACCAGATTT TAAGTAACTG GAGAGAAGAA CGATACCAGG ATGACATCAA AGCACGACAG 
ATGATGCATG AGGCATTGCA ACTCCGCCTG AATTTGGTGG GAGGAATGTT TGACACAGTG 
CAGAGGAGCA CCCAGTGGAC AACAGACTGG GCGCTTCTCC TCCTTCAGAT CATTACTTCT 
GGAACCGTTG ACATGCACAC CAACAATGAA TTATTCACAA CAGTTCTTGA CATGCTGGGT 
GTTTTAATCA ATGGAACATT GGCCTCTGAC CTGTCGAATG CATCCCCAGG GGGATCTGAA 
GAGAACAAAC GTGCATACAT GAACTTAGTC AAGAAACTGA AAAAAGAGCT GGGGGACAAG 
CGGTCAGAAA GTATTGACAA AGTTCGACAG CTGTTACCTT TGCCGAAACA GACATGTGAC 
ATCATTACCT GTGAACCTAT GGGCTCCTTG ATTGACACAA AGGGAAACAA AATTGCTGGC 
TTCGATTCTA TAGATAAAAA ACAGGGTCTC CAGGTCTCCA CGAAGCAGAA GGTGTCCCCG 
TGGGATTTGT TTGAGGGTCA GAAGAACCCG GCTCCTCTGT CCTGGGCCTG GTTCGGGACT 
GTCCGAGTGG ACCGGAAGGT GATCAAGTAT GAGGAGCAAC ATCACCTCCT CCTGTATCAC 
ACGCACCCCA CGCCCAAGCC CCGGAGTTAC TACCTCGAGC CACTGCCCCT GCCTCCCGAA 
GAGGAAGAGG AAGAGCCCAC ATCTCCCATC TCTCAGGAAC CTGAGAGGAA ATCAGCTGAG 
CTGTCAGATC AGGGAAAAAC CGCAACAGAT GAAGAGAAGA AGACAAAGGG AAGGAAGCGC 
AAGACAAAGT CCAGCTCAAG GGTTGATGAG TATCCGCAGA GCAGCATGTA CCGAGTGCCT 
CCCAACTACT CACCCATCTC CTCCCAGGTG ATGCACCACC CGCAGTCCGC CCTGTGGGGC 
TACAACCTCA TGGGTCAGCC CCAGCAGCCC GGCTTCTTCC TTCAGAACCA GTCTGTCACT 
CCAGGTGGTT CCCGGTTGGA CCCCGCTGGC TCCTTCGTCC CCACCAACAC CAAGCAAGCT 
TTGTCCAACA TGCTCCAGCG GCGCTCGGGG GCCATGATGC AGCCGCCCTC TCTGCATGCC 
ATCACATCGC AGCAGCAGTT GATACAGATG AAGCTTCTGC AGCAGCAGCA GCAACAGCGG 
CTTCTCAGGC AAGCCCAGAC GCGGCCTTTC CAACAGGGCC AGCCAGGGGA CCAGGCTGCT 
CTCTTTGCTG CTCAGGCACG GCCCTCCCCT CAGCTCCCCC AGTATCCAGG ACTGCAGCAA 
GCACAGACCA TGCCCCAGGG CTACACAATG TATGGAACCC AGATGCCTTT GCAGCAGACC 
CCTCAGCAGC AGGCTGGCAG CGTGGTCCTG TCTCCTACCT ATAATTCCAG AACCTATCCG 
GCCGCTCACT CCAACCCAGC GCTCATGGAA AGACTCAGAC AGATGCAGCA GCCGCCGAGC 
GGCTATGTTC AGCAGCAGGC GTCACCATAC CTGCATCCCC TGACCGGCTC CCAGAGGCTG 
AATCATCAGT CGCTTCAGCA GAGCCCTCTG GTGGGTGGAG GAATCGATGC GGTGCTGACT 
TCCGCACATC CAAACCTTCC CTCAGTGCCC TTGTCTCAGG ACCCGATGAG ACCCAGACAG 
CCACAGGTTC GACAGCAGCA GAGGCTTCTC CAGATGCAGC AGCCCCAGCA GCCCCCCCAG 
CCCCAGCAGC CTCCCCAGCC TCAGGGGTCC TCGCAGACCC AGAGTCAGAC CCTTGGTCTC 
CAAACAATGC AGCCCCAGCA GCCTCTGTTC CCCAGGCAAG GCTTGCAGCA GACCCAGCAG 
CAACAGCAGA CGGCTGCCCT GGTGCGGCAG CTCCAGAAGC AGCTTTCCAG TAACCAGCCA 
CAGCAAGGAG TGACTCCCTA CGGGCATCCT TCACACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799788.1
CDS	268..6849
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEEQTIMAHMGIDSGTTNIFDEVDKNDFKTDFGSEFPIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_010801486.2
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801486.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GGAACAGACT ATTATGGCAC ATATGGGCAT TGACTCAGGA 
ACTACTAATA TTTTTGATGA AGTAGACAAG AATGACTTTA AAACTGACTT TGGTTCGGAA 
TTTCCAATTT TTTCTCCCAT GCCTGGAGAG TCCTGTGAGA ATGCCAACCC TTCTCTGGGC 
AGAAGAATGT CAGTTAATGG TGAGAAGCTG TTGAAGAGAG AAAAACCAAG GGAGTTGATT 
TTTCCATCTA ATTATGACCT CCTACGACAC CTGCAGTATG CAACCCATTT TCCCATACCT 
CTGGATGAAT CTTCAAGTCA TGAATGTAAC CAGCGCACAA TCCTTCTTTA TGGAGTGGGA 
AAAGAGCGTG ACGAAGCCAG ACATCAGCTG AAGAAGATTA CCAAAGATAT TTTGAAAATC 
CTAAATAAGA AGAGCAACAC AGAGACAGGG GTTGGAGATG AAGGACAGAA AGCCAGGAAG 
AGCAAACAGG AAGCATTTCC GACCCTGGAG ACTGTGTTCA CAAAACTTCA ACTCCTTTCA 
TATTTTGATC AGCATCAAGT GACATCTCAG ATTTCTAACA ATGTGCTAGA ACAAATCACA 
AGCTTTGCAT CAGGAACATC ATACCATCTC CCATTGGCTC ACCACATTCA GCTCATCTTT 
GATCTCATGG AGCCAGCACT TAATATTAAT GGACTCATTG ACTTCGCAAT ACAGCTCCTG 
AACGAACTGA GTGTTGTGGA GGCAGAACTG CTCCTGAAGT CCTCCAGCCT GGCAGGAAGC 
TACACGACAG GACTGTGTGT CTGCATCGTG GCTGTTCTCA GGCGTTACCA CAGTTGTCTG 
ATCCTGAATC CTGATCAGAC AGCCCAGGTG TTTGAAGGAT TGTGTGGTGT GGTAAAGCAC 
GTTGTGAACC CCTCAGAGTG CTCTTCCCCC GAAAGATGCA TCTTAGCCTA CCTCTACGAC 
CTCTACGTGT CATGTAGCCA CCTCAGAAGT AAATTTGGAG ACCTCTTCAG TAGTGCCTGT 
TCAAAAGTAA AGCAAACCAT ATATAACAAT GTGATTCCTG CAAATTCTAA CTTGCGATGG 
GATCCAGACT TCATGATGGA TTTTATTGAG AATCCCTCGG CCCGTAGCGT CAACTACTCA 
GTGCTGGGCA AGATCCTTAG TGACAATGCA GCCAATCGCT ACAGCTTCGT CTGCAACACT 
CTCATGAATG TATGTATGGG TCATCAGGAT GCTGGAAGGA TTAATGACAT AGCCAATTTC 
TCCTCCGAGC TGACAGCCTG CTGCACTGTT CTCAGTTCAG AATGGCTGGG GGTCCTGAAG 
GCTCTTTGTT GTTCTTCAAA TCATGTGTGG GGCTTTAATG ATGTTCTTTG CACTGTCGAT 
GTGAGTGACC TTTCATTCCA TGATTCATTA GCTACTTTCA TTGCTATTCT GATAGCACGA 
CAGTGTTTCT CCCTGGAGGA CGTCGTGCAA CACGTCGCAC TCCCCTCTCT CCTCGCCGCA 
GCTTGCGGAG ATGCAGATGC AGAGCCTGGG GCAAGAATGA CATGCCGTCT CCTGCTTCAT 
CTCTTTCGAG CTCCCCAGGC CTGCCTGTTA CCTCAAGCAA CTGGCAAACC TTTCCCGGGA 
ATAAGATCGT CTTGTGACAG ACACCTCTTA GCTGCTGCTC ACAACAGCAT TGAAGTGGGA 
GCCGTGTTTG CTGTCTTAAA AGCAATTATG ATGCTTGGAG ATGCCAAAAT TGGCAATAAC 
AGTGTCAGCT CTTTAAAAAA TGATGACTTC ACCATGAGGG GTTTACGACG TGATGGGAAT 
GCTGAGGATA TCTGGACTGC CTCACAAAAT TCAAAATCCT GTGGGAAAAG CATTTCCATA 
GAAACGGCCA ATTTAAGAGA ATATGCTAGA TATGTACTGA GGACCATCTG TCAACAGGAA 
TGGGTAGGAG AACATTGTTT AAAAGAACCT GAGAGATTAT GCACAGACAA AGAACTTATT 
CTGGACCCTG TGCTCTCAAA TATGCAAGCA CAGAAATTAC TGCAGCTGAT CTGTTATCCT 
CATGGCATTA AGGAATGTAC GGAGGGGGAC AACCTCCAAA GACAGCACAT TAAACGCATT 
CTTCAGAATC TTGAGCAGTG GACGCTGAGG CAGTCCTGGC TGGAGCTTCA GTTAATGATC 
AAACAGTGCT TGAAGGACCC TGGCTCTGGT TCGGTGGCTG AAATGAACAA CCTGCTGGAC 
AATATTGCCA AGGCAACAAT AGAGGTGTTC CAGCAGTCCG CTGACCTAAA CAATAACTCT 
TCTAATGCTG GCATGGGCCT CTTCAACCCA AATGGAATTG GAAGTACTGA TACAAGTAGC 
ACGAGACAGA ATGGAATAAA GACATTTCTA AGTTCCTCTG AACGCAGGGG TGTGTGGCTG 
GTGGCCCCCC TAATTGCCAG GCTGCCGACT TCTGTGCAGG GAAGAGTGTT GAAAGCAGCT 
GGGGAAGAGC TGGAGAAAGG ACAGCACTTG GGTTCTTCTT CCAAAAAGGA AAGAGACAGA 
CAAAAGCAGA AAAGTATGTC TCTTTTGAGT CAACAACCAT TCCTCTCACT GGTCCTTACC 
TGCCTTAAGG GACAAGATGA ACAACGAGAA GGCCTTCTAA CATCTCTGCA GAATCAAGTT 
AACCAGATTT TAAGTAACTG GAGAGAAGAA CGATACCAGG ATGACATCAA AGCACGACAG 
ATGATGCATG AGGCATTGCA ACTCCGCCTG AATTTGGTGG GAGGAATGTT TGACACAGTG 
CAGAGGAGCA CCCAGTGGAC AACAGACTGG GCGCTTCTCC TCCTTCAGAT CATTACTTCT 
GGAACCGTTG ACATGCACAC CAACAATGAA TTATTCACAA CAGTTCTTGA CATGCTGGGT 
GTTTTAATCA ATGGAACATT GGCCTCTGAC CTGTCGAATG CATCCCCAGG GGGATCTGAA 
GAGAACAAAC GTGCATACAT GAACTTAGTC AAGAAACTGA AAAAAGAGCT GGGGGACAAG 
CGGTCAGAAA GTATTGACAA AGTTCGACAG CTGTTACCTT TGCCGAAACA GACATGTGAC 
ATCATTACCT GTGAACCTAT GGGCTCCTTG ATTGACACAA AGGGAAACAA AATTGCTGGC 
TTCGATTCTA TAGATAAAAA ACAGGGTCTC CAGGTCTCCA CGAAGCAGAA GGTGTCCCCG 
TGGGATTTGT TTGAGGGTCA GAAGAACCCG GCTCCTCTGT CCTGGGCCTG GTTCGGGACT 
GTCCGAGTGG ACCGGAAGGT GATCAAGTAT GAGGAGCAAC ATCACCTCCT CCTGTATCAC 
ACGCACCCCA CGCCCAAGCC CCGGAGTTAC TACCTCGAGC CACTGCCCCT GCCTCCCGAA 
GAGGAAGAGG AAGAGCCCAC ATCTCCCATC TCTCAGGAAC CTGAGAGGAA ATCAGCTGAG 
CTGTCAGATC AGGGAAAAAC CGCAACAGAT GAAGAGAAGA AGACAAAGGG AAGGAAGCGC 
AAGACAAAGT CCAGCTCAAG GGTTGATGAG TATCCGCAGA GCAGCATGTA CCGAGTGCCT 
CCCAACTACT CACCCATCTC CTCCCAGGTG ATGCACCACC CGCAGTCCGC CCTGTGGGGC 
TACAACCTCA TGGGTCAGCC CCAGCAGCCC GGCTTCTTCC TTCAGAACCA GTCTGTCACT 
CCAGGTGGTT CCCGGTTGGA CCCCGCTGGC TCCTTCGTCC CCACCAACAC CAAGCAAGCT 
TTGTCCAACA TGCTCCAGCG GCGCTCGGGG GCCATGATGC AGCCGCCCTC TCTGCATGCC 
ATCACATCGC AGCAGCAGTT GATACAGATG AAGCTTCTGC AGCAGCAGCA GCAACAGCGG 
CTTCTCAGGC AAGCCCAGAC GCGGCCTTTC CAACAGGGCC AGCCAGGGGA CCAGGCTGCT 
CTCTTTGCTG CTCAGGCACG GCCCTCCCCT CAGCTCCCCC AGTATCCAGG ACTGCAGCAA 
GCACAGACCA TGCCCCAGGG CTACACAATG TATGGAACCC AGATGCCTTT GCAGCAGACC 
CCTCAGCAGC AGGCTGGCAG CGTGGTCCTG TCTCCTACCT ATAATTCCAG AACCTATCCG 
GCCGCTCACT CCAACCCAGC GCTCATGGAA AGACTCAGAC AGATGCAGCA GCCGCCGAGC 
GGCTATGTTC AGCAGCAGGC GTCACCATAC CTGCATCCCC TGACCGGCTC CCAGAGGCTG 
AATCATCAGT CGCTTCAGCA GAGCCCTCTG GTGGGTGGAG GAATCGATGC GGTGCTGACT 
TCCGCACATC CAAACCTTCC CTCAGTGCCC TTGTCTCAGG ACCCGATGAG ACCCAGACAG 
CCACAGGTTC GACAGCAGCA GAGGCTTCTC CAGATGCAGC AGCCCCAGCA GCCCCCCCAG 
CCCCAGCAGC CTCCCCAGCC TCAGGGGTCC TCGCAGACCC AGAGTCAGAC CCTTGGTCTC 
CAAACAATGC AGCCCCAGCA GCCTCTGTTC CCCAGGCAAG GCTTGCAGCA GACCCAGCAG 
CAACAGCAGA CGGCTGCCCT GGTGCGGCAG CTCCAGAAGC AGCTTTCCAG TAACCAGCCA 
CAGCAAGGAG TGACTCCCTA CGGGCATCCT TCACACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_010801485.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799787.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X1, mRNA.

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CloneID	OBa54150
Clone ID Related Accession (Same CDS sequence)	XM_010801486.2 , XM_010801485.2
Accession Version	XM_010801485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103819.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GGAACAGACT ATTATGGCAC ATATGGGCAT TGACTCAGGA 
ACTACTAATA TTTTTGATGA AGTAGACAAG AATGACTTTA AAACTGACTT TGGTTCGGAA 
TTTCCAATTT TTTCTCCCAT GCCTGGAGAG TCCTGTGAGA ATGCCAACCC TTCTCTGGGC 
AGAAGAATGT CAGTTAATGG TGAGAAGCTG TTGAAGAGAG AAAAACCAAG GGAGTTGATT 
TTTCCATCTA ATTATGACCT CCTACGACAC CTGCAGTATG CAACCCATTT TCCCATACCT 
CTGGATGAAT CTTCAAGTCA TGAATGTAAC CAGCGCACAA TCCTTCTTTA TGGAGTGGGA 
AAAGAGCGTG ACGAAGCCAG ACATCAGCTG AAGAAGATTA CCAAAGATAT TTTGAAAATC 
CTAAATAAGA AGAGCAACAC AGAGACAGGG GTTGGAGATG AAGGACAGAA AGCCAGGAAG 
AGCAAACAGG AAGCATTTCC GACCCTGGAG ACTGTGTTCA CAAAACTTCA ACTCCTTTCA 
TATTTTGATC AGCATCAAGT GACATCTCAG ATTTCTAACA ATGTGCTAGA ACAAATCACA 
AGCTTTGCAT CAGGAACATC ATACCATCTC CCATTGGCTC ACCACATTCA GCTCATCTTT 
GATCTCATGG AGCCAGCACT TAATATTAAT GGACTCATTG ACTTCGCAAT ACAGCTCCTG 
AACGAACTGA GTGTTGTGGA GGCAGAACTG CTCCTGAAGT CCTCCAGCCT GGCAGGAAGC 
TACACGACAG GACTGTGTGT CTGCATCGTG GCTGTTCTCA GGCGTTACCA CAGTTGTCTG 
ATCCTGAATC CTGATCAGAC AGCCCAGGTG TTTGAAGGAT TGTGTGGTGT GGTAAAGCAC 
GTTGTGAACC CCTCAGAGTG CTCTTCCCCC GAAAGATGCA TCTTAGCCTA CCTCTACGAC 
CTCTACGTGT CATGTAGCCA CCTCAGAAGT AAATTTGGAG ACCTCTTCAG TAGTGCCTGT 
TCAAAAGTAA AGCAAACCAT ATATAACAAT GTGATTCCTG CAAATTCTAA CTTGCGATGG 
GATCCAGACT TCATGATGGA TTTTATTGAG AATCCCTCGG CCCGTAGCGT CAACTACTCA 
GTGCTGGGCA AGATCCTTAG TGACAATGCA GCCAATCGCT ACAGCTTCGT CTGCAACACT 
CTCATGAATG TATGTATGGG TCATCAGGAT GCTGGAAGGA TTAATGACAT AGCCAATTTC 
TCCTCCGAGC TGACAGCCTG CTGCACTGTT CTCAGTTCAG AATGGCTGGG GGTCCTGAAG 
GCTCTTTGTT GTTCTTCAAA TCATGTGTGG GGCTTTAATG ATGTTCTTTG CACTGTCGAT 
GTGAGTGACC TTTCATTCCA TGATTCATTA GCTACTTTCA TTGCTATTCT GATAGCACGA 
CAGTGTTTCT CCCTGGAGGA CGTCGTGCAA CACGTCGCAC TCCCCTCTCT CCTCGCCGCA 
GCTTGCGGAG ATGCAGATGC AGAGCCTGGG GCAAGAATGA CATGCCGTCT CCTGCTTCAT 
CTCTTTCGAG CTCCCCAGGC CTGCCTGTTA CCTCAAGCAA CTGGCAAACC TTTCCCGGGA 
ATAAGATCGT CTTGTGACAG ACACCTCTTA GCTGCTGCTC ACAACAGCAT TGAAGTGGGA 
GCCGTGTTTG CTGTCTTAAA AGCAATTATG ATGCTTGGAG ATGCCAAAAT TGGCAATAAC 
AGTGTCAGCT CTTTAAAAAA TGATGACTTC ACCATGAGGG GTTTACGACG TGATGGGAAT 
GCTGAGGATA TCTGGACTGC CTCACAAAAT TCAAAATCCT GTGGGAAAAG CATTTCCATA 
GAAACGGCCA ATTTAAGAGA ATATGCTAGA TATGTACTGA GGACCATCTG TCAACAGGAA 
TGGGTAGGAG AACATTGTTT AAAAGAACCT GAGAGATTAT GCACAGACAA AGAACTTATT 
CTGGACCCTG TGCTCTCAAA TATGCAAGCA CAGAAATTAC TGCAGCTGAT CTGTTATCCT 
CATGGCATTA AGGAATGTAC GGAGGGGGAC AACCTCCAAA GACAGCACAT TAAACGCATT 
CTTCAGAATC TTGAGCAGTG GACGCTGAGG CAGTCCTGGC TGGAGCTTCA GTTAATGATC 
AAACAGTGCT TGAAGGACCC TGGCTCTGGT TCGGTGGCTG AAATGAACAA CCTGCTGGAC 
AATATTGCCA AGGCAACAAT AGAGGTGTTC CAGCAGTCCG CTGACCTAAA CAATAACTCT 
TCTAATGCTG GCATGGGCCT CTTCAACCCA AATGGAATTG GAAGTACTGA TACAAGTAGC 
ACGAGACAGA ATGGAATAAA GACATTTCTA AGTTCCTCTG AACGCAGGGG TGTGTGGCTG 
GTGGCCCCCC TAATTGCCAG GCTGCCGACT TCTGTGCAGG GAAGAGTGTT GAAAGCAGCT 
GGGGAAGAGC TGGAGAAAGG ACAGCACTTG GGTTCTTCTT CCAAAAAGGA AAGAGACAGA 
CAAAAGCAGA AAAGTATGTC TCTTTTGAGT CAACAACCAT TCCTCTCACT GGTCCTTACC 
TGCCTTAAGG GACAAGATGA ACAACGAGAA GGCCTTCTAA CATCTCTGCA GAATCAAGTT 
AACCAGATTT TAAGTAACTG GAGAGAAGAA CGATACCAGG ATGACATCAA AGCACGACAG 
ATGATGCATG AGGCATTGCA ACTCCGCCTG AATTTGGTGG GAGGAATGTT TGACACAGTG 
CAGAGGAGCA CCCAGTGGAC AACAGACTGG GCGCTTCTCC TCCTTCAGAT CATTACTTCT 
GGAACCGTTG ACATGCACAC CAACAATGAA TTATTCACAA CAGTTCTTGA CATGCTGGGT 
GTTTTAATCA ATGGAACATT GGCCTCTGAC CTGTCGAATG CATCCCCAGG GGGATCTGAA 
GAGAACAAAC GTGCATACAT GAACTTAGTC AAGAAACTGA AAAAAGAGCT GGGGGACAAG 
CGGTCAGAAA GTATTGACAA AGTTCGACAG CTGTTACCTT TGCCGAAACA GACATGTGAC 
ATCATTACCT GTGAACCTAT GGGCTCCTTG ATTGACACAA AGGGAAACAA AATTGCTGGC 
TTCGATTCTA TAGATAAAAA ACAGGGTCTC CAGGTCTCCA CGAAGCAGAA GGTGTCCCCG 
TGGGATTTGT TTGAGGGTCA GAAGAACCCG GCTCCTCTGT CCTGGGCCTG GTTCGGGACT 
GTCCGAGTGG ACCGGAAGGT GATCAAGTAT GAGGAGCAAC ATCACCTCCT CCTGTATCAC 
ACGCACCCCA CGCCCAAGCC CCGGAGTTAC TACCTCGAGC CACTGCCCCT GCCTCCCGAA 
GAGGAAGAGG AAGAGCCCAC ATCTCCCATC TCTCAGGAAC CTGAGAGGAA ATCAGCTGAG 
CTGTCAGATC AGGGAAAAAC CGCAACAGAT GAAGAGAAGA AGACAAAGGG AAGGAAGCGC 
AAGACAAAGT CCAGCTCAAG GGTTGATGAG TATCCGCAGA GCAGCATGTA CCGAGTGCCT 
CCCAACTACT CACCCATCTC CTCCCAGGTG ATGCACCACC CGCAGTCCGC CCTGTGGGGC 
TACAACCTCA TGGGTCAGCC CCAGCAGCCC GGCTTCTTCC TTCAGAACCA GTCTGTCACT 
CCAGGTGGTT CCCGGTTGGA CCCCGCTGGC TCCTTCGTCC CCACCAACAC CAAGCAAGCT 
TTGTCCAACA TGCTCCAGCG GCGCTCGGGG GCCATGATGC AGCCGCCCTC TCTGCATGCC 
ATCACATCGC AGCAGCAGTT GATACAGATG AAGCTTCTGC AGCAGCAGCA GCAACAGCGG 
CTTCTCAGGC AAGCCCAGAC GCGGCCTTTC CAACAGGGCC AGCCAGGGGA CCAGGCTGCT 
CTCTTTGCTG CTCAGGCACG GCCCTCCCCT CAGCTCCCCC AGTATCCAGG ACTGCAGCAA 
GCACAGACCA TGCCCCAGGG CTACACAATG TATGGAACCC AGATGCCTTT GCAGCAGACC 
CCTCAGCAGC AGGCTGGCAG CGTGGTCCTG TCTCCTACCT ATAATTCCAG AACCTATCCG 
GCCGCTCACT CCAACCCAGC GCTCATGGAA AGACTCAGAC AGATGCAGCA GCCGCCGAGC 
GGCTATGTTC AGCAGCAGGC GTCACCATAC CTGCATCCCC TGACCGGCTC CCAGAGGCTG 
AATCATCAGT CGCTTCAGCA GAGCCCTCTG GTGGGTGGAG GAATCGATGC GGTGCTGACT 
TCCGCACATC CAAACCTTCC CTCAGTGCCC TTGTCTCAGG ACCCGATGAG ACCCAGACAG 
CCACAGGTTC GACAGCAGCA GAGGCTTCTC CAGATGCAGC AGCCCCAGCA GCCCCCCCAG 
CCCCAGCAGC CTCCCCAGCC TCAGGGGTCC TCGCAGACCC AGAGTCAGAC CCTTGGTCTC 
CAAACAATGC AGCCCCAGCA GCCTCTGTTC CCCAGGCAAG GCTTGCAGCA GACCCAGCAG 
CAACAGCAGA CGGCTGCCCT GGTGCGGCAG CTCCAGAAGC AGCTTTCCAG TAACCAGCCA 
CAGCAAGGAG TGACTCCCTA CGGGCATCCT TCACACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799787.1
CDS	321..6902
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEEQTIMAHMGIDSGTTNIFDEVDKNDFKTDFGSEFPIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_010801485.2
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801485.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GGAACAGACT ATTATGGCAC ATATGGGCAT TGACTCAGGA 
ACTACTAATA TTTTTGATGA AGTAGACAAG AATGACTTTA AAACTGACTT TGGTTCGGAA 
TTTCCAATTT TTTCTCCCAT GCCTGGAGAG TCCTGTGAGA ATGCCAACCC TTCTCTGGGC 
AGAAGAATGT CAGTTAATGG TGAGAAGCTG TTGAAGAGAG AAAAACCAAG GGAGTTGATT 
TTTCCATCTA ATTATGACCT CCTACGACAC CTGCAGTATG CAACCCATTT TCCCATACCT 
CTGGATGAAT CTTCAAGTCA TGAATGTAAC CAGCGCACAA TCCTTCTTTA TGGAGTGGGA 
AAAGAGCGTG ACGAAGCCAG ACATCAGCTG AAGAAGATTA CCAAAGATAT TTTGAAAATC 
CTAAATAAGA AGAGCAACAC AGAGACAGGG GTTGGAGATG AAGGACAGAA AGCCAGGAAG 
AGCAAACAGG AAGCATTTCC GACCCTGGAG ACTGTGTTCA CAAAACTTCA ACTCCTTTCA 
TATTTTGATC AGCATCAAGT GACATCTCAG ATTTCTAACA ATGTGCTAGA ACAAATCACA 
AGCTTTGCAT CAGGAACATC ATACCATCTC CCATTGGCTC ACCACATTCA GCTCATCTTT 
GATCTCATGG AGCCAGCACT TAATATTAAT GGACTCATTG ACTTCGCAAT ACAGCTCCTG 
AACGAACTGA GTGTTGTGGA GGCAGAACTG CTCCTGAAGT CCTCCAGCCT GGCAGGAAGC 
TACACGACAG GACTGTGTGT CTGCATCGTG GCTGTTCTCA GGCGTTACCA CAGTTGTCTG 
ATCCTGAATC CTGATCAGAC AGCCCAGGTG TTTGAAGGAT TGTGTGGTGT GGTAAAGCAC 
GTTGTGAACC CCTCAGAGTG CTCTTCCCCC GAAAGATGCA TCTTAGCCTA CCTCTACGAC 
CTCTACGTGT CATGTAGCCA CCTCAGAAGT AAATTTGGAG ACCTCTTCAG TAGTGCCTGT 
TCAAAAGTAA AGCAAACCAT ATATAACAAT GTGATTCCTG CAAATTCTAA CTTGCGATGG 
GATCCAGACT TCATGATGGA TTTTATTGAG AATCCCTCGG CCCGTAGCGT CAACTACTCA 
GTGCTGGGCA AGATCCTTAG TGACAATGCA GCCAATCGCT ACAGCTTCGT CTGCAACACT 
CTCATGAATG TATGTATGGG TCATCAGGAT GCTGGAAGGA TTAATGACAT AGCCAATTTC 
TCCTCCGAGC TGACAGCCTG CTGCACTGTT CTCAGTTCAG AATGGCTGGG GGTCCTGAAG 
GCTCTTTGTT GTTCTTCAAA TCATGTGTGG GGCTTTAATG ATGTTCTTTG CACTGTCGAT 
GTGAGTGACC TTTCATTCCA TGATTCATTA GCTACTTTCA TTGCTATTCT GATAGCACGA 
CAGTGTTTCT CCCTGGAGGA CGTCGTGCAA CACGTCGCAC TCCCCTCTCT CCTCGCCGCA 
GCTTGCGGAG ATGCAGATGC AGAGCCTGGG GCAAGAATGA CATGCCGTCT CCTGCTTCAT 
CTCTTTCGAG CTCCCCAGGC CTGCCTGTTA CCTCAAGCAA CTGGCAAACC TTTCCCGGGA 
ATAAGATCGT CTTGTGACAG ACACCTCTTA GCTGCTGCTC ACAACAGCAT TGAAGTGGGA 
GCCGTGTTTG CTGTCTTAAA AGCAATTATG ATGCTTGGAG ATGCCAAAAT TGGCAATAAC 
AGTGTCAGCT CTTTAAAAAA TGATGACTTC ACCATGAGGG GTTTACGACG TGATGGGAAT 
GCTGAGGATA TCTGGACTGC CTCACAAAAT TCAAAATCCT GTGGGAAAAG CATTTCCATA 
GAAACGGCCA ATTTAAGAGA ATATGCTAGA TATGTACTGA GGACCATCTG TCAACAGGAA 
TGGGTAGGAG AACATTGTTT AAAAGAACCT GAGAGATTAT GCACAGACAA AGAACTTATT 
CTGGACCCTG TGCTCTCAAA TATGCAAGCA CAGAAATTAC TGCAGCTGAT CTGTTATCCT 
CATGGCATTA AGGAATGTAC GGAGGGGGAC AACCTCCAAA GACAGCACAT TAAACGCATT 
CTTCAGAATC TTGAGCAGTG GACGCTGAGG CAGTCCTGGC TGGAGCTTCA GTTAATGATC 
AAACAGTGCT TGAAGGACCC TGGCTCTGGT TCGGTGGCTG AAATGAACAA CCTGCTGGAC 
AATATTGCCA AGGCAACAAT AGAGGTGTTC CAGCAGTCCG CTGACCTAAA CAATAACTCT 
TCTAATGCTG GCATGGGCCT CTTCAACCCA AATGGAATTG GAAGTACTGA TACAAGTAGC 
ACGAGACAGA ATGGAATAAA GACATTTCTA AGTTCCTCTG AACGCAGGGG TGTGTGGCTG 
GTGGCCCCCC TAATTGCCAG GCTGCCGACT TCTGTGCAGG GAAGAGTGTT GAAAGCAGCT 
GGGGAAGAGC TGGAGAAAGG ACAGCACTTG GGTTCTTCTT CCAAAAAGGA AAGAGACAGA 
CAAAAGCAGA AAAGTATGTC TCTTTTGAGT CAACAACCAT TCCTCTCACT GGTCCTTACC 
TGCCTTAAGG GACAAGATGA ACAACGAGAA GGCCTTCTAA CATCTCTGCA GAATCAAGTT 
AACCAGATTT TAAGTAACTG GAGAGAAGAA CGATACCAGG ATGACATCAA AGCACGACAG 
ATGATGCATG AGGCATTGCA ACTCCGCCTG AATTTGGTGG GAGGAATGTT TGACACAGTG 
CAGAGGAGCA CCCAGTGGAC AACAGACTGG GCGCTTCTCC TCCTTCAGAT CATTACTTCT 
GGAACCGTTG ACATGCACAC CAACAATGAA TTATTCACAA CAGTTCTTGA CATGCTGGGT 
GTTTTAATCA ATGGAACATT GGCCTCTGAC CTGTCGAATG CATCCCCAGG GGGATCTGAA 
GAGAACAAAC GTGCATACAT GAACTTAGTC AAGAAACTGA AAAAAGAGCT GGGGGACAAG 
CGGTCAGAAA GTATTGACAA AGTTCGACAG CTGTTACCTT TGCCGAAACA GACATGTGAC 
ATCATTACCT GTGAACCTAT GGGCTCCTTG ATTGACACAA AGGGAAACAA AATTGCTGGC 
TTCGATTCTA TAGATAAAAA ACAGGGTCTC CAGGTCTCCA CGAAGCAGAA GGTGTCCCCG 
TGGGATTTGT TTGAGGGTCA GAAGAACCCG GCTCCTCTGT CCTGGGCCTG GTTCGGGACT 
GTCCGAGTGG ACCGGAAGGT GATCAAGTAT GAGGAGCAAC ATCACCTCCT CCTGTATCAC 
ACGCACCCCA CGCCCAAGCC CCGGAGTTAC TACCTCGAGC CACTGCCCCT GCCTCCCGAA 
GAGGAAGAGG AAGAGCCCAC ATCTCCCATC TCTCAGGAAC CTGAGAGGAA ATCAGCTGAG 
CTGTCAGATC AGGGAAAAAC CGCAACAGAT GAAGAGAAGA AGACAAAGGG AAGGAAGCGC 
AAGACAAAGT CCAGCTCAAG GGTTGATGAG TATCCGCAGA GCAGCATGTA CCGAGTGCCT 
CCCAACTACT CACCCATCTC CTCCCAGGTG ATGCACCACC CGCAGTCCGC CCTGTGGGGC 
TACAACCTCA TGGGTCAGCC CCAGCAGCCC GGCTTCTTCC TTCAGAACCA GTCTGTCACT 
CCAGGTGGTT CCCGGTTGGA CCCCGCTGGC TCCTTCGTCC CCACCAACAC CAAGCAAGCT 
TTGTCCAACA TGCTCCAGCG GCGCTCGGGG GCCATGATGC AGCCGCCCTC TCTGCATGCC 
ATCACATCGC AGCAGCAGTT GATACAGATG AAGCTTCTGC AGCAGCAGCA GCAACAGCGG 
CTTCTCAGGC AAGCCCAGAC GCGGCCTTTC CAACAGGGCC AGCCAGGGGA CCAGGCTGCT 
CTCTTTGCTG CTCAGGCACG GCCCTCCCCT CAGCTCCCCC AGTATCCAGG ACTGCAGCAA 
GCACAGACCA TGCCCCAGGG CTACACAATG TATGGAACCC AGATGCCTTT GCAGCAGACC 
CCTCAGCAGC AGGCTGGCAG CGTGGTCCTG TCTCCTACCT ATAATTCCAG AACCTATCCG 
GCCGCTCACT CCAACCCAGC GCTCATGGAA AGACTCAGAC AGATGCAGCA GCCGCCGAGC 
GGCTATGTTC AGCAGCAGGC GTCACCATAC CTGCATCCCC TGACCGGCTC CCAGAGGCTG 
AATCATCAGT CGCTTCAGCA GAGCCCTCTG GTGGGTGGAG GAATCGATGC GGTGCTGACT 
TCCGCACATC CAAACCTTCC CTCAGTGCCC TTGTCTCAGG ACCCGATGAG ACCCAGACAG 
CCACAGGTTC GACAGCAGCA GAGGCTTCTC CAGATGCAGC AGCCCCAGCA GCCCCCCCAG 
CCCCAGCAGC CTCCCCAGCC TCAGGGGTCC TCGCAGACCC AGAGTCAGAC CCTTGGTCTC 
CAAACAATGC AGCCCCAGCA GCCTCTGTTC CCCAGGCAAG GCTTGCAGCA GACCCAGCAG 
CAACAGCAGA CGGCTGCCCT GGTGCGGCAG CTCCAGAAGC AGCTTTCCAG TAACCAGCCA 
CAGCAAGGAG TGACTCCCTA CGGGCATCCT TCACACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_010801487.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799789.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X3, mRNA.

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CloneID	OBa54151
Clone ID Related Accession (Same CDS sequence)	XM_010801487.2
Accession Version	XM_010801487.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103819.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GGAACAGACT ATTATGGCAC ATATGGGCAT TGACTCAGGA 
ACTACTAATA TTTTTGATGA AGTAGACAAG AATGACTTTA AAACTGACTT TGGTTCGGAA 
TTTCCAATTT TTTCTCCCAT GCCTGGAGAG TCCTGTGAGA ATGCCAACCC TTCTCTGGGC 
AGAAGAATGT CAGTTAATGG TGAGAAGCTG TTGAAGAGAG AAAAACCAAG GGAGTTGATT 
TTTCCATCTA ATTATGACCT CCTACGACAC CTGCAGTATG CAACCCATTT TCCCATACCT 
CTGGATGAAT CTTCAAGTCA TGAATGTAAC CAGCGCACAA TCCTTCTTTA TGGAGTGGGA 
AAAGAGCGTG ACGAAGCCAG ACATCAGCTG AAGAAGATTA CCAAAGATAT TTTGAAAATC 
CTAAATAAGA AGAGCAACAC AGAGACAGGG GTTGGAGATG AAGGACAGAA AGCCAGGAAG 
AGCAAACAGG AAGCATTTCC GACCCTGGAG ACTGTGTTCA CAAAACTTCA ACTCCTTTCA 
TATTTTGATC AGCATCAAGT GACATCTCAG ATTTCTAACA ATGTGCTAGA ACAAATCACA 
AGCTTTGCAT CAGGAACATC ATACCATCTC CCATTGGCTC ACCACATTCA GCTCATCTTT 
GATCTCATGG AGCCAGCACT TAATATTAAT GGACTCATTG ACTTCGCAAT ACAGCTCCTG 
AACGAACTGA GTGTTGTGGA GGCAGAACTG CTCCTGAAGT CCTCCAGCCT GGCAGGAAGC 
TACACGACAG GACTGTGTGT CTGCATCGTG GCTGTTCTCA GGCGTTACCA CAGTTGTCTG 
ATCCTGAATC CTGATCAGAC AGCCCAGGTG TTTGAAGGAT TGTGTGGTGT GGTAAAGCAC 
GTTGTGAACC CCTCAGAGTG CTCTTCCCCC GAAAGATGCA TCTTAGCCTA CCTCTACGAC 
CTCTACGTGT CATGTAGCCA CCTCAGAAGT AAATTTGGAG ACCTCTTCAG TGCCTGTTCA 
AAAGTAAAGC AAACCATATA TAACAATGTG ATTCCTGCAA ATTCTAACTT GCGATGGGAT 
CCAGACTTCA TGATGGATTT TATTGAGAAT CCCTCGGCCC GTAGCGTCAA CTACTCAGTG 
CTGGGCAAGA TCCTTAGTGA CAATGCAGCC AATCGCTACA GCTTCGTCTG CAACACTCTC 
ATGAATGTAT GTATGGGTCA TCAGGATGCT GGAAGGATTA ATGACATAGC CAATTTCTCC 
TCCGAGCTGA CAGCCTGCTG CACTGTTCTC AGTTCAGAAT GGCTGGGGGT CCTGAAGGCT 
CTTTGTTGTT CTTCAAATCA TGTGTGGGGC TTTAATGATG TTCTTTGCAC TGTCGATGTG 
AGTGACCTTT CATTCCATGA TTCATTAGCT ACTTTCATTG CTATTCTGAT AGCACGACAG 
TGTTTCTCCC TGGAGGACGT CGTGCAACAC GTCGCACTCC CCTCTCTCCT CGCCGCAGCT 
TGCGGAGATG CAGATGCAGA GCCTGGGGCA AGAATGACAT GCCGTCTCCT GCTTCATCTC 
TTTCGAGCTC CCCAGGCCTG CCTGTTACCT CAAGCAACTG GCAAACCTTT CCCGGGAATA 
AGATCGTCTT GTGACAGACA CCTCTTAGCT GCTGCTCACA ACAGCATTGA AGTGGGAGCC 
GTGTTTGCTG TCTTAAAAGC AATTATGATG CTTGGAGATG CCAAAATTGG CAATAACAGT 
GTCAGCTCTT TAAAAAATGA TGACTTCACC ATGAGGGGTT TACGACGTGA TGGGAATGCT 
GAGGATATCT GGACTGCCTC ACAAAATTCA AAATCCTGTG GGAAAAGCAT TTCCATAGAA 
ACGGCCAATT TAAGAGAATA TGCTAGATAT GTACTGAGGA CCATCTGTCA ACAGGAATGG 
GTAGGAGAAC ATTGTTTAAA AGAACCTGAG AGATTATGCA CAGACAAAGA ACTTATTCTG 
GACCCTGTGC TCTCAAATAT GCAAGCACAG AAATTACTGC AGCTGATCTG TTATCCTCAT 
GGCATTAAGG AATGTACGGA GGGGGACAAC CTCCAAAGAC AGCACATTAA ACGCATTCTT 
CAGAATCTTG AGCAGTGGAC GCTGAGGCAG TCCTGGCTGG AGCTTCAGTT AATGATCAAA 
CAGTGCTTGA AGGACCCTGG CTCTGGTTCG GTGGCTGAAA TGAACAACCT GCTGGACAAT 
ATTGCCAAGG CAACAATAGA GGTGTTCCAG CAGTCCGCTG ACCTAAACAA TAACTCTTCT 
AATGCTGGCA TGGGCCTCTT CAACCCAAAT GGAATTGGAA GTACTGATAC AAGTAGCACG 
AGACAGAATG GAATAAAGAC ATTTCTAAGT TCCTCTGAAC GCAGGGGTGT GTGGCTGGTG 
GCCCCCCTAA TTGCCAGGCT GCCGACTTCT GTGCAGGGAA GAGTGTTGAA AGCAGCTGGG 
GAAGAGCTGG AGAAAGGACA GCACTTGGGT TCTTCTTCCA AAAAGGAAAG AGACAGACAA 
AAGCAGAAAA GTATGTCTCT TTTGAGTCAA CAACCATTCC TCTCACTGGT CCTTACCTGC 
CTTAAGGGAC AAGATGAACA ACGAGAAGGC CTTCTAACAT CTCTGCAGAA TCAAGTTAAC 
CAGATTTTAA GTAACTGGAG AGAAGAACGA TACCAGGATG ACATCAAAGC ACGACAGATG 
ATGCATGAGG CATTGCAACT CCGCCTGAAT TTGGTGGGAG GAATGTTTGA CACAGTGCAG 
AGGAGCACCC AGTGGACAAC AGACTGGGCG CTTCTCCTCC TTCAGATCAT TACTTCTGGA 
ACCGTTGACA TGCACACCAA CAATGAATTA TTCACAACAG TTCTTGACAT GCTGGGTGTT 
TTAATCAATG GAACATTGGC CTCTGACCTG TCGAATGCAT CCCCAGGGGG ATCTGAAGAG 
AACAAACGTG CATACATGAA CTTAGTCAAG AAACTGAAAA AAGAGCTGGG GGACAAGCGG 
TCAGAAAGTA TTGACAAAGT TCGACAGCTG TTACCTTTGC CGAAACAGAC ATGTGACATC 
ATTACCTGTG AACCTATGGG CTCCTTGATT GACACAAAGG GAAACAAAAT TGCTGGCTTC 
GATTCTATAG ATAAAAAACA GGGTCTCCAG GTCTCCACGA AGCAGAAGGT GTCCCCGTGG 
GATTTGTTTG AGGGTCAGAA GAACCCGGCT CCTCTGTCCT GGGCCTGGTT CGGGACTGTC 
CGAGTGGACC GGAAGGTGAT CAAGTATGAG GAGCAACATC ACCTCCTCCT GTATCACACG 
CACCCCACGC CCAAGCCCCG GAGTTACTAC CTCGAGCCAC TGCCCCTGCC TCCCGAAGAG 
GAAGAGGAAG AGCCCACATC TCCCATCTCT CAGGAACCTG AGAGGAAATC AGCTGAGCTG 
TCAGATCAGG GAAAAACCGC AACAGATGAA GAGAAGAAGA CAAAGGGAAG GAAGCGCAAG 
ACAAAGTCCA GCTCAAGGGT TGATGAGTAT CCGCAGAGCA GCATGTACCG AGTGCCTCCC 
AACTACTCAC CCATCTCCTC CCAGGTGATG CACCACCCGC AGTCCGCCCT GTGGGGCTAC 
AACCTCATGG GTCAGCCCCA GCAGCCCGGC TTCTTCCTTC AGAACCAGTC TGTCACTCCA 
GGTGGTTCCC GGTTGGACCC CGCTGGCTCC TTCGTCCCCA CCAACACCAA GCAAGCTTTG 
TCCAACATGC TCCAGCGGCG CTCGGGGGCC ATGATGCAGC CGCCCTCTCT GCATGCCATC 
ACATCGCAGC AGCAGTTGAT ACAGATGAAG CTTCTGCAGC AGCAGCAGCA ACAGCGGCTT 
CTCAGGCAAG CCCAGACGCG GCCTTTCCAA CAGGGCCAGC CAGGGGACCA GGCTGCTCTC 
TTTGCTGCTC AGGCACGGCC CTCCCCTCAG CTCCCCCAGT ATCCAGGACT GCAGCAAGCA 
CAGACCATGC CCCAGGGCTA CACAATGTAT GGAACCCAGA TGCCTTTGCA GCAGACCCCT 
CAGCAGCAGG CTGGCAGCGT GGTCCTGTCT CCTACCTATA ATTCCAGAAC CTATCCGGCC 
GCTCACTCCA ACCCAGCGCT CATGGAAAGA CTCAGACAGA TGCAGCAGCC GCCGAGCGGC 
TATGTTCAGC AGCAGGCGTC ACCATACCTG CATCCCCTGA CCGGCTCCCA GAGGCTGAAT 
CATCAGTCGC TTCAGCAGAG CCCTCTGGTG GGTGGAGGAA TCGATGCGGT GCTGACTTCC 
GCACATCCAA ACCTTCCCTC AGTGCCCTTG TCTCAGGACC CGATGAGACC CAGACAGCCA 
CAGGTTCGAC AGCAGCAGAG GCTTCTCCAG ATGCAGCAGC CCCAGCAGCC CCCCCAGCCC 
CAGCAGCCTC CCCAGCCTCA GGGGTCCTCG CAGACCCAGA GTCAGACCCT TGGTCTCCAA 
ACAATGCAGC CCCAGCAGCC TCTGTTCCCC AGGCAAGGCT TGCAGCAGAC CCAGCAGCAA 
CAGCAGACGG CTGCCCTGGT GCGGCAGCTC CAGAAGCAGC TTTCCAGTAA CCAGCCACAG 
CAAGGAGTGA CTCCCTACGG GCATCCTTCA CACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799789.1
CDS	321..6899
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEEQTIMAHMGIDSGTTNIFDEVDKNDFKTDFGSEFPIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_010801487.2
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801487.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGGTT GCCCCCAACG ATGAAGCTGT GGTGACGCTG 
CTGTGTGAAT GGGCTGTGAG CTGTAAGCGG TCAGGCAAGC ACAGGGCGAT GGCTGTGGCG 
AAACTCCTGG AGAAGAGACA AGCGGAAATC GAGGCAGAGA GATGTGGTGA ATCAGAAGTC 
TTAGATGAGA AGGAGTCCAT TTCCTCAGCC TCCCTCGCTG GATCTAGTTT ACCTGTTTTC 
CAGAATGTTC TTCTAAGGTT TTTAGATACA CAGGCCCCCT CGTTGTCGGA TCCAAATAGC 
GAATGTGAGA AGGTGGAGTT TGTCAACCTG GTGCTGCTCT TCTGTGAGTT CATCCGGCAT 
GACGTCTTCT CCCACGATGC CTACATGTGC ACCCTCATCT CTCGGGGAGA CCTGTCTGTC 
ACCGCCTCCA CGCGACCGCG GTCGCCAGCA GGAGAGAATA CGGATGAGCA TTATCCAAAG 
GACCACGACA TGAAAATGGA GGAACAGACT ATTATGGCAC ATATGGGCAT TGACTCAGGA 
ACTACTAATA TTTTTGATGA AGTAGACAAG AATGACTTTA AAACTGACTT TGGTTCGGAA 
TTTCCAATTT TTTCTCCCAT GCCTGGAGAG TCCTGTGAGA ATGCCAACCC TTCTCTGGGC 
AGAAGAATGT CAGTTAATGG TGAGAAGCTG TTGAAGAGAG AAAAACCAAG GGAGTTGATT 
TTTCCATCTA ATTATGACCT CCTACGACAC CTGCAGTATG CAACCCATTT TCCCATACCT 
CTGGATGAAT CTTCAAGTCA TGAATGTAAC CAGCGCACAA TCCTTCTTTA TGGAGTGGGA 
AAAGAGCGTG ACGAAGCCAG ACATCAGCTG AAGAAGATTA CCAAAGATAT TTTGAAAATC 
CTAAATAAGA AGAGCAACAC AGAGACAGGG GTTGGAGATG AAGGACAGAA AGCCAGGAAG 
AGCAAACAGG AAGCATTTCC GACCCTGGAG ACTGTGTTCA CAAAACTTCA ACTCCTTTCA 
TATTTTGATC AGCATCAAGT GACATCTCAG ATTTCTAACA ATGTGCTAGA ACAAATCACA 
AGCTTTGCAT CAGGAACATC ATACCATCTC CCATTGGCTC ACCACATTCA GCTCATCTTT 
GATCTCATGG AGCCAGCACT TAATATTAAT GGACTCATTG ACTTCGCAAT ACAGCTCCTG 
AACGAACTGA GTGTTGTGGA GGCAGAACTG CTCCTGAAGT CCTCCAGCCT GGCAGGAAGC 
TACACGACAG GACTGTGTGT CTGCATCGTG GCTGTTCTCA GGCGTTACCA CAGTTGTCTG 
ATCCTGAATC CTGATCAGAC AGCCCAGGTG TTTGAAGGAT TGTGTGGTGT GGTAAAGCAC 
GTTGTGAACC CCTCAGAGTG CTCTTCCCCC GAAAGATGCA TCTTAGCCTA CCTCTACGAC 
CTCTACGTGT CATGTAGCCA CCTCAGAAGT AAATTTGGAG ACCTCTTCAG TGCCTGTTCA 
AAAGTAAAGC AAACCATATA TAACAATGTG ATTCCTGCAA ATTCTAACTT GCGATGGGAT 
CCAGACTTCA TGATGGATTT TATTGAGAAT CCCTCGGCCC GTAGCGTCAA CTACTCAGTG 
CTGGGCAAGA TCCTTAGTGA CAATGCAGCC AATCGCTACA GCTTCGTCTG CAACACTCTC 
ATGAATGTAT GTATGGGTCA TCAGGATGCT GGAAGGATTA ATGACATAGC CAATTTCTCC 
TCCGAGCTGA CAGCCTGCTG CACTGTTCTC AGTTCAGAAT GGCTGGGGGT CCTGAAGGCT 
CTTTGTTGTT CTTCAAATCA TGTGTGGGGC TTTAATGATG TTCTTTGCAC TGTCGATGTG 
AGTGACCTTT CATTCCATGA TTCATTAGCT ACTTTCATTG CTATTCTGAT AGCACGACAG 
TGTTTCTCCC TGGAGGACGT CGTGCAACAC GTCGCACTCC CCTCTCTCCT CGCCGCAGCT 
TGCGGAGATG CAGATGCAGA GCCTGGGGCA AGAATGACAT GCCGTCTCCT GCTTCATCTC 
TTTCGAGCTC CCCAGGCCTG CCTGTTACCT CAAGCAACTG GCAAACCTTT CCCGGGAATA 
AGATCGTCTT GTGACAGACA CCTCTTAGCT GCTGCTCACA ACAGCATTGA AGTGGGAGCC 
GTGTTTGCTG TCTTAAAAGC AATTATGATG CTTGGAGATG CCAAAATTGG CAATAACAGT 
GTCAGCTCTT TAAAAAATGA TGACTTCACC ATGAGGGGTT TACGACGTGA TGGGAATGCT 
GAGGATATCT GGACTGCCTC ACAAAATTCA AAATCCTGTG GGAAAAGCAT TTCCATAGAA 
ACGGCCAATT TAAGAGAATA TGCTAGATAT GTACTGAGGA CCATCTGTCA ACAGGAATGG 
GTAGGAGAAC ATTGTTTAAA AGAACCTGAG AGATTATGCA CAGACAAAGA ACTTATTCTG 
GACCCTGTGC TCTCAAATAT GCAAGCACAG AAATTACTGC AGCTGATCTG TTATCCTCAT 
GGCATTAAGG AATGTACGGA GGGGGACAAC CTCCAAAGAC AGCACATTAA ACGCATTCTT 
CAGAATCTTG AGCAGTGGAC GCTGAGGCAG TCCTGGCTGG AGCTTCAGTT AATGATCAAA 
CAGTGCTTGA AGGACCCTGG CTCTGGTTCG GTGGCTGAAA TGAACAACCT GCTGGACAAT 
ATTGCCAAGG CAACAATAGA GGTGTTCCAG CAGTCCGCTG ACCTAAACAA TAACTCTTCT 
AATGCTGGCA TGGGCCTCTT CAACCCAAAT GGAATTGGAA GTACTGATAC AAGTAGCACG 
AGACAGAATG GAATAAAGAC ATTTCTAAGT TCCTCTGAAC GCAGGGGTGT GTGGCTGGTG 
GCCCCCCTAA TTGCCAGGCT GCCGACTTCT GTGCAGGGAA GAGTGTTGAA AGCAGCTGGG 
GAAGAGCTGG AGAAAGGACA GCACTTGGGT TCTTCTTCCA AAAAGGAAAG AGACAGACAA 
AAGCAGAAAA GTATGTCTCT TTTGAGTCAA CAACCATTCC TCTCACTGGT CCTTACCTGC 
CTTAAGGGAC AAGATGAACA ACGAGAAGGC CTTCTAACAT CTCTGCAGAA TCAAGTTAAC 
CAGATTTTAA GTAACTGGAG AGAAGAACGA TACCAGGATG ACATCAAAGC ACGACAGATG 
ATGCATGAGG CATTGCAACT CCGCCTGAAT TTGGTGGGAG GAATGTTTGA CACAGTGCAG 
AGGAGCACCC AGTGGACAAC AGACTGGGCG CTTCTCCTCC TTCAGATCAT TACTTCTGGA 
ACCGTTGACA TGCACACCAA CAATGAATTA TTCACAACAG TTCTTGACAT GCTGGGTGTT 
TTAATCAATG GAACATTGGC CTCTGACCTG TCGAATGCAT CCCCAGGGGG ATCTGAAGAG 
AACAAACGTG CATACATGAA CTTAGTCAAG AAACTGAAAA AAGAGCTGGG GGACAAGCGG 
TCAGAAAGTA TTGACAAAGT TCGACAGCTG TTACCTTTGC CGAAACAGAC ATGTGACATC 
ATTACCTGTG AACCTATGGG CTCCTTGATT GACACAAAGG GAAACAAAAT TGCTGGCTTC 
GATTCTATAG ATAAAAAACA GGGTCTCCAG GTCTCCACGA AGCAGAAGGT GTCCCCGTGG 
GATTTGTTTG AGGGTCAGAA GAACCCGGCT CCTCTGTCCT GGGCCTGGTT CGGGACTGTC 
CGAGTGGACC GGAAGGTGAT CAAGTATGAG GAGCAACATC ACCTCCTCCT GTATCACACG 
CACCCCACGC CCAAGCCCCG GAGTTACTAC CTCGAGCCAC TGCCCCTGCC TCCCGAAGAG 
GAAGAGGAAG AGCCCACATC TCCCATCTCT CAGGAACCTG AGAGGAAATC AGCTGAGCTG 
TCAGATCAGG GAAAAACCGC AACAGATGAA GAGAAGAAGA CAAAGGGAAG GAAGCGCAAG 
ACAAAGTCCA GCTCAAGGGT TGATGAGTAT CCGCAGAGCA GCATGTACCG AGTGCCTCCC 
AACTACTCAC CCATCTCCTC CCAGGTGATG CACCACCCGC AGTCCGCCCT GTGGGGCTAC 
AACCTCATGG GTCAGCCCCA GCAGCCCGGC TTCTTCCTTC AGAACCAGTC TGTCACTCCA 
GGTGGTTCCC GGTTGGACCC CGCTGGCTCC TTCGTCCCCA CCAACACCAA GCAAGCTTTG 
TCCAACATGC TCCAGCGGCG CTCGGGGGCC ATGATGCAGC CGCCCTCTCT GCATGCCATC 
ACATCGCAGC AGCAGTTGAT ACAGATGAAG CTTCTGCAGC AGCAGCAGCA ACAGCGGCTT 
CTCAGGCAAG CCCAGACGCG GCCTTTCCAA CAGGGCCAGC CAGGGGACCA GGCTGCTCTC 
TTTGCTGCTC AGGCACGGCC CTCCCCTCAG CTCCCCCAGT ATCCAGGACT GCAGCAAGCA 
CAGACCATGC CCCAGGGCTA CACAATGTAT GGAACCCAGA TGCCTTTGCA GCAGACCCCT 
CAGCAGCAGG CTGGCAGCGT GGTCCTGTCT CCTACCTATA ATTCCAGAAC CTATCCGGCC 
GCTCACTCCA ACCCAGCGCT CATGGAAAGA CTCAGACAGA TGCAGCAGCC GCCGAGCGGC 
TATGTTCAGC AGCAGGCGTC ACCATACCTG CATCCCCTGA CCGGCTCCCA GAGGCTGAAT 
CATCAGTCGC TTCAGCAGAG CCCTCTGGTG GGTGGAGGAA TCGATGCGGT GCTGACTTCC 
GCACATCCAA ACCTTCCCTC AGTGCCCTTG TCTCAGGACC CGATGAGACC CAGACAGCCA 
CAGGTTCGAC AGCAGCAGAG GCTTCTCCAG ATGCAGCAGC CCCAGCAGCC CCCCCAGCCC 
CAGCAGCCTC CCCAGCCTCA GGGGTCCTCG CAGACCCAGA GTCAGACCCT TGGTCTCCAA 
ACAATGCAGC CCCAGCAGCC TCTGTTCCCC AGGCAAGGCT TGCAGCAGAC CCAGCAGCAA 
CAGCAGACGG CTGCCCTGGT GCGGCAGCTC CAGAAGCAGC TTTCCAGTAA CCAGCCACAG 
CAAGGAGTGA CTCCCTACGG GCATCCTTCA CACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_010801488.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799790.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X4, mRNA.

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CloneID	OBa54152
Clone ID Related Accession (Same CDS sequence)	XM_010801488.2
Accession Version	XM_010801488.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103819.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801488.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CCATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC GAAGTATTCA GTGCCAATGG TTCGAGCAAC ATGGCTGATC 
AAGATGACGT GTGCCTATTA CTCTGCTATT TCTGAAGCGA AAATTAAGAA ACGTCCGGCC 
ACTGATCCCA ATTTGGAGTG GACGCAAATA TCTACCAGGT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCCG ACTTCTACCA CCTGGCTTCC AGCGCGGGCG ATGGCCCTGT CCCTGTGCCA 
CCCGACGTGG AGCAGGCCAT GAGGCAGTGG GAGTACAACG AAAAGTTGGC ATTCCACATG 
TTCCAGGAAG GAATGCTGGA AAAGCATGAA TATTTGACAT GGATATTGGA TGTGTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTAT TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTCCAGTC GGCCTACCTG TCCCGTCGTC TCGCCTATTT CTGTGCCCGG 
CGTCTGGCGT TGCTGCTGAG CGACAGCCCC AGCCTCCTTG CCGCCCACTC GCCCCATGTG 
ATAATAGGCC CAAACAGCTC GAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGGC 
ATGAGCCCTG TGCAGCTGGC CTTCTCCGAC TTTCTTTCCT GTGCACAGCA CGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTTACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTACT CCACAAACGA CAATAAGAGC GCGAACCCGG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGCCT CCCCATGCCG GGAGGAAACA CGGCTTTCAA CCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCG ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AGGTATTGGA TCGACACTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGACAACCA AGAGGTTGCC 
CCCAACGATG AAGCTGTGGT GACGCTGCTG TGTGAATGGG CTGTGAGCTG TAAGCGGTCA 
GGCAAGCACA GGGCGATGGC TGTGGCGAAA CTCCTGGAGA AGAGACAAGC GGAAATCGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCCATTTC CTCAGCCTCC 
CTCGCTGGAT CTAGTTTACC TGTTTTCCAG AATGTTCTTC TAAGGTTTTT AGATACACAG 
GCCCCCTCGT TGTCGGATCC AAATAGCGAA TGTGAGAAGG TGGAGTTTGT CAACCTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGGCATGAC GTCTTCTCCC ACGATGCCTA CATGTGCACC 
CTCATCTCTC GGGGAGACCT GTCTGTCACC GCCTCCACGC GACCGCGGTC GCCAGCAGGA 
GAGAATACGG ATGAGCATTA TCCAAAGGAC CACGACATGA AAATGGAGGA ACAGACTATT 
ATGGCACATA TGGGCATTGA CTCAGGAACT ACTAATATTT TTGATGAAGT AGACAAGAAT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCCATGCC TGGAGAGTCC 
TGTGAGAATG CCAACCCTTC TCTGGGCAGA AGAATGTCAG TTAATGGTGA GAAGCTGTTG 
AAGAGAGAAA AACCAAGGGA GTTGATTTTT CCATCTAATT ATGACCTCCT ACGACACCTG 
CAGTATGCAA CCCATTTTCC CATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTTTATGG AGTGGGAAAA GAGCGTGACG AAGCCAGACA TCAGCTGAAG 
AAGATTACCA AAGATATTTT GAAAATCCTA AATAAGAAGA GCAACACAGA GACAGGGGTT 
GGAGATGAAG GACAGAAAGC CAGGAAGAGC AAACAGGAAG CATTTCCGAC CCTGGAGACT 
GTGTTCACAA AACTTCAACT CCTTTCATAT TTTGATCAGC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCATCAG GAACATCATA CCATCTCCCA 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTTAA TATTAATGGA 
CTCATTGACT TCGCAATACA GCTCCTGAAC GAACTGAGTG TTGTGGAGGC AGAACTGCTC 
CTGAAGTCCT CCAGCCTGGC AGGAAGCTAC ACGACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GTTACCACAG TTGTCTGATC CTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGATTGT GTGGTGTGGT AAAGCACGTT GTGAACCCCT CAGAGTGCTC TTCCCCCGAA 
AGATGCATCT TAGCCTACCT CTACGACCTC TACGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTAAAGC AAACCATATA TAACAATGTG 
ATTCCTGCAA ATTCTAACTT GCGATGGGAT CCAGACTTCA TGATGGATTT TATTGAGAAT 
CCCTCGGCCC GTAGCGTCAA CTACTCAGTG CTGGGCAAGA TCCTTAGTGA CAATGCAGCC 
AATCGCTACA GCTTCGTCTG CAACACTCTC ATGAATGTAT GTATGGGTCA TCAGGATGCT 
GGAAGGATTA ATGACATAGC CAATTTCTCC TCCGAGCTGA CAGCCTGCTG CACTGTTCTC 
AGTTCAGAAT GGCTGGGGGT CCTGAAGGCT CTTTGTTGTT CTTCAAATCA TGTGTGGGGC 
TTTAATGATG TTCTTTGCAC TGTCGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTCTCCC TGGAGGACGT CGTGCAACAC 
GTCGCACTCC CCTCTCTCCT CGCCGCAGCT TGCGGAGATG CAGATGCAGA GCCTGGGGCA 
AGAATGACAT GCCGTCTCCT GCTTCATCTC TTTCGAGCTC CCCAGGCCTG CCTGTTACCT 
CAAGCAACTG GCAAACCTTT CCCGGGAATA AGATCGTCTT GTGACAGACA CCTCTTAGCT 
GCTGCTCACA ACAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGAGATG CCAAAATTGG CAATAACAGT GTCAGCTCTT TAAAAAATGA TGACTTCACC 
ATGAGGGGTT TACGACGTGA TGGGAATGCT GAGGATATCT GGACTGCCTC ACAAAATTCA 
AAATCCTGTG GGAAAAGCAT TTCCATAGAA ACGGCCAATT TAAGAGAATA TGCTAGATAT 
GTACTGAGGA CCATCTGTCA ACAGGAATGG GTAGGAGAAC ATTGTTTAAA AGAACCTGAG 
AGATTATGCA CAGACAAAGA ACTTATTCTG GACCCTGTGC TCTCAAATAT GCAAGCACAG 
AAATTACTGC AGCTGATCTG TTATCCTCAT GGCATTAAGG AATGTACGGA GGGGGACAAC 
CTCCAAAGAC AGCACATTAA ACGCATTCTT CAGAATCTTG AGCAGTGGAC GCTGAGGCAG 
TCCTGGCTGG AGCTTCAGTT AATGATCAAA CAGTGCTTGA AGGACCCTGG CTCTGGTTCG 
GTGGCTGAAA TGAACAACCT GCTGGACAAT ATTGCCAAGG CAACAATAGA GGTGTTCCAG 
CAGTCCGCTG ACCTAAACAA TAACTCTTCT AATGCTGGCA TGGGCCTCTT CAACCCAAAT 
GGAATTGGAA GTACTGATAC AAGTAGCACG AGACAGAATG GAATAAAGAC ATTTCTAAGT 
TCCTCTGAAC GCAGGGGTGT GTGGCTGGTG GCCCCCCTAA TTGCCAGGCT GCCGACTTCT 
GTGCAGGGAA GAGTGTTGAA AGCAGCTGGG GAAGAGCTGG AGAAAGGACA GCACTTGGGT 
TCTTCTTCCA AAAAGGAAAG AGACAGACAA AAGCAGAAAA GTATGTCTCT TTTGAGTCAA 
CAACCATTCC TCTCACTGGT CCTTACCTGC CTTAAGGGAC AAGATGAACA ACGAGAAGGC 
CTTCTAACAT CTCTGCAGAA TCAAGTTAAC CAGATTTTAA GTAACTGGAG AGAAGAACGA 
TACCAGGATG ACATCAAAGC ACGACAGATG ATGCATGAGG CATTGCAACT CCGCCTGAAT 
TTGGTGGGAG GAATGTTTGA CACAGTGCAG AGGAGCACCC AGTGGACAAC AGACTGGGCG 
CTTCTCCTCC TTCAGATCAT TACTTCTGGA ACCGTTGACA TGCACACCAA CAATGAATTA 
TTCACAACAG TTCTTGACAT GCTGGGTGTT TTAATCAATG GAACATTGGC CTCTGACCTG 
TCGAATGCAT CCCCAGGGGG ATCTGAAGAG AACAAACGTG CATACATGAA CTTAGTCAAG 
AAACTGAAAA AAGAGCTGGG GGACAAGCGG TCAGAAAGTA TTGACAAAGT TCGACAGCTG 
TTACCTTTGC CGAAACAGAC ATGTGACATC ATTACCTGTG AACCTATGGG CTCCTTGATT 
GACACAAAGG GAAACAAAAT TGCTGGCTTC GATTCTATAG ATAAAAAACA GGGTCTCCAG 
GTCTCCACGA AGCAGAAGGT GTCCCCGTGG GATTTGTTTG AGGGTCAGAA GAACCCGGCT 
CCTCTGTCCT GGGCCTGGTT CGGGACTGTC CGAGTGGACC GGAAGGTGAT CAAGTATGAG 
GAGCAACATC ACCTCCTCCT GTATCACACG CACCCCACGC CCAAGCCCCG GAGTTACTAC 
CTCGAGCCAC TGCCCCTGCC TCCCGAAGAG GAAGAGGAAG AGCCCACATC TCCCATCTCT 
CAGGAACCTG AGAGGAAATC AGCTGAGCTG TCAGATCAGG GAAAAACCGC AACAGATGAA 
GAGAAGAAGA CAAAGGGAAG GAAGCGCAAG ACAAAGTCCA GCTCAAGGGT TGATGAGTAT 
CCGCAGAGCA GCATGTACCG AGTGCCTCCC AACTACTCAC CCATCTCCTC CCAGGTGATG 
CACCACCCGC AGTCCGCCCT GTGGGGCTAC AACCTCATGG GTCAGCCCCA GCAGCCCGGC 
TTCTTCCTTC AGAACCAGTC TGTCACTCCA GGTGGTTCCC GGTTGGACCC CGCTGGCTCC 
TTCGTCCCCA CCAACACCAA GCAAGCTTTG TCCAACATGC TCCAGCGGCG CTCGGGGGCC 
ATGATGCAGC CGCCCTCTCT GCATGCCATC ACATCGCAGC AGCAGTTGAT ACAGATGAAG 
CTTCTGCAGC AGCAGCAGCA ACAGCGGCTT CTCAGGCAAG CCCAGACGCG GCCTTTCCAA 
CAGGGCCAGC CAGGGGACCA GGCTGCTCTC TTTGCTGCTC AGGCACGGCC CTCCCCTCAG 
CTCCCCCAGT ATCCAGGACT GCAGCAAGCA CAGACCATGC CCCAGGGCTA CACAATGTAT 
GGAACCCAGA TGCCTTTGCA GCAGACCCCT CAGCAGCAGG CTGGCAGCGT GGTCCTGTCT 
CCTACCTATA ATTCCAGAAC CTATCCGGCC GCTCACTCCA ACCCAGCGCT CATGGAAAGA 
CTCAGACAGA TGCAGCAGCC GCCGAGCGGC TATGTTCAGC AGCAGGCGTC ACCATACCTG 
CATCCCCTGA CCGGCTCCCA GAGGCTGAAT CATCAGTCGC TTCAGCAGAG CCCTCTGGTG 
GGTGGAGGAA TCGATGCGGT GCTGACTTCC GCACATCCAA ACCTTCCCTC AGTGCCCTTG 
TCTCAGGACC CGATGAGACC CAGACAGCCA CAGGTTCGAC AGCAGCAGAG GCTTCTCCAG 
ATGCAGCAGC CCCAGCAGCC CCCCCAGCCC CAGCAGCCTC CCCAGCCTCA GGGGTCCTCG 
CAGACCCAGA GTCAGACCCT TGGTCTCCAA ACAATGCAGC CCCAGCAGCC TCTGTTCCCC 
AGGCAAGGCT TGCAGCAGAC CCAGCAGCAA CAGCAGACGG CTGCCCTGGT GCGGCAGCTC 
CAGAAGCAGC TTTCCAGTAA CCAGCCACAG CAAGGAGTGA CTCCCTACGG GCATCCTTCA 
CACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799790.1
CDS	321..6869
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEEQTIMAHMGIDSGTTNIFDEVDKNDFKTDFGSEFPIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_010801488.2
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801488.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CCATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC GAAGTATTCA GTGCCAATGG TTCGAGCAAC ATGGCTGATC 
AAGATGACGT GTGCCTATTA CTCTGCTATT TCTGAAGCGA AAATTAAGAA ACGTCCGGCC 
ACTGATCCCA ATTTGGAGTG GACGCAAATA TCTACCAGGT ATCTTCGAGA GCAGTTGGCC 
AAGATTTCCG ACTTCTACCA CCTGGCTTCC AGCGCGGGCG ATGGCCCTGT CCCTGTGCCA 
CCCGACGTGG AGCAGGCCAT GAGGCAGTGG GAGTACAACG AAAAGTTGGC ATTCCACATG 
TTCCAGGAAG GAATGCTGGA AAAGCATGAA TATTTGACAT GGATATTGGA TGTGTTAGAA 
AAGATCAGAC CAATGGATGA TGATCTTCTT AAACTCTTAT TACCACTAAT GCTGCAGTAT 
TCAGATGAGT TTGTCCAGTC GGCCTACCTG TCCCGTCGTC TCGCCTATTT CTGTGCCCGG 
CGTCTGGCGT TGCTGCTGAG CGACAGCCCC AGCCTCCTTG CCGCCCACTC GCCCCATGTG 
ATAATAGGCC CAAACAGCTC GAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGGC 
ATGAGCCCTG TGCAGCTGGC CTTCTCCGAC TTTCTTTCCT GTGCACAGCA CGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTTACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTACT CCACAAACGA CAATAAGAGC GCGAACCCGG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGCCT CCCCATGCCG GGAGGAAACA CGGCTTTCAA CCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCG ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AGGTATTGGA TCGACACTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGACAACCA AGAGGTTGCC 
CCCAACGATG AAGCTGTGGT GACGCTGCTG TGTGAATGGG CTGTGAGCTG TAAGCGGTCA 
GGCAAGCACA GGGCGATGGC TGTGGCGAAA CTCCTGGAGA AGAGACAAGC GGAAATCGAG 
GCAGAGAGAT GTGGTGAATC AGAAGTCTTA GATGAGAAGG AGTCCATTTC CTCAGCCTCC 
CTCGCTGGAT CTAGTTTACC TGTTTTCCAG AATGTTCTTC TAAGGTTTTT AGATACACAG 
GCCCCCTCGT TGTCGGATCC AAATAGCGAA TGTGAGAAGG TGGAGTTTGT CAACCTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGGCATGAC GTCTTCTCCC ACGATGCCTA CATGTGCACC 
CTCATCTCTC GGGGAGACCT GTCTGTCACC GCCTCCACGC GACCGCGGTC GCCAGCAGGA 
GAGAATACGG ATGAGCATTA TCCAAAGGAC CACGACATGA AAATGGAGGA ACAGACTATT 
ATGGCACATA TGGGCATTGA CTCAGGAACT ACTAATATTT TTGATGAAGT AGACAAGAAT 
GACTTTAAAA CTGACTTTGG TTCGGAATTT CCAATTTTTT CTCCCATGCC TGGAGAGTCC 
TGTGAGAATG CCAACCCTTC TCTGGGCAGA AGAATGTCAG TTAATGGTGA GAAGCTGTTG 
AAGAGAGAAA AACCAAGGGA GTTGATTTTT CCATCTAATT ATGACCTCCT ACGACACCTG 
CAGTATGCAA CCCATTTTCC CATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TTCTTTATGG AGTGGGAAAA GAGCGTGACG AAGCCAGACA TCAGCTGAAG 
AAGATTACCA AAGATATTTT GAAAATCCTA AATAAGAAGA GCAACACAGA GACAGGGGTT 
GGAGATGAAG GACAGAAAGC CAGGAAGAGC AAACAGGAAG CATTTCCGAC CCTGGAGACT 
GTGTTCACAA AACTTCAACT CCTTTCATAT TTTGATCAGC ATCAAGTGAC ATCTCAGATT 
TCTAACAATG TGCTAGAACA AATCACAAGC TTTGCATCAG GAACATCATA CCATCTCCCA 
TTGGCTCACC ACATTCAGCT CATCTTTGAT CTCATGGAGC CAGCACTTAA TATTAATGGA 
CTCATTGACT TCGCAATACA GCTCCTGAAC GAACTGAGTG TTGTGGAGGC AGAACTGCTC 
CTGAAGTCCT CCAGCCTGGC AGGAAGCTAC ACGACAGGAC TGTGTGTCTG CATCGTGGCT 
GTTCTCAGGC GTTACCACAG TTGTCTGATC CTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGATTGT GTGGTGTGGT AAAGCACGTT GTGAACCCCT CAGAGTGCTC TTCCCCCGAA 
AGATGCATCT TAGCCTACCT CTACGACCTC TACGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTAAAGC AAACCATATA TAACAATGTG 
ATTCCTGCAA ATTCTAACTT GCGATGGGAT CCAGACTTCA TGATGGATTT TATTGAGAAT 
CCCTCGGCCC GTAGCGTCAA CTACTCAGTG CTGGGCAAGA TCCTTAGTGA CAATGCAGCC 
AATCGCTACA GCTTCGTCTG CAACACTCTC ATGAATGTAT GTATGGGTCA TCAGGATGCT 
GGAAGGATTA ATGACATAGC CAATTTCTCC TCCGAGCTGA CAGCCTGCTG CACTGTTCTC 
AGTTCAGAAT GGCTGGGGGT CCTGAAGGCT CTTTGTTGTT CTTCAAATCA TGTGTGGGGC 
TTTAATGATG TTCTTTGCAC TGTCGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTCTCCC TGGAGGACGT CGTGCAACAC 
GTCGCACTCC CCTCTCTCCT CGCCGCAGCT TGCGGAGATG CAGATGCAGA GCCTGGGGCA 
AGAATGACAT GCCGTCTCCT GCTTCATCTC TTTCGAGCTC CCCAGGCCTG CCTGTTACCT 
CAAGCAACTG GCAAACCTTT CCCGGGAATA AGATCGTCTT GTGACAGACA CCTCTTAGCT 
GCTGCTCACA ACAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGAGATG CCAAAATTGG CAATAACAGT GTCAGCTCTT TAAAAAATGA TGACTTCACC 
ATGAGGGGTT TACGACGTGA TGGGAATGCT GAGGATATCT GGACTGCCTC ACAAAATTCA 
AAATCCTGTG GGAAAAGCAT TTCCATAGAA ACGGCCAATT TAAGAGAATA TGCTAGATAT 
GTACTGAGGA CCATCTGTCA ACAGGAATGG GTAGGAGAAC ATTGTTTAAA AGAACCTGAG 
AGATTATGCA CAGACAAAGA ACTTATTCTG GACCCTGTGC TCTCAAATAT GCAAGCACAG 
AAATTACTGC AGCTGATCTG TTATCCTCAT GGCATTAAGG AATGTACGGA GGGGGACAAC 
CTCCAAAGAC AGCACATTAA ACGCATTCTT CAGAATCTTG AGCAGTGGAC GCTGAGGCAG 
TCCTGGCTGG AGCTTCAGTT AATGATCAAA CAGTGCTTGA AGGACCCTGG CTCTGGTTCG 
GTGGCTGAAA TGAACAACCT GCTGGACAAT ATTGCCAAGG CAACAATAGA GGTGTTCCAG 
CAGTCCGCTG ACCTAAACAA TAACTCTTCT AATGCTGGCA TGGGCCTCTT CAACCCAAAT 
GGAATTGGAA GTACTGATAC AAGTAGCACG AGACAGAATG GAATAAAGAC ATTTCTAAGT 
TCCTCTGAAC GCAGGGGTGT GTGGCTGGTG GCCCCCCTAA TTGCCAGGCT GCCGACTTCT 
GTGCAGGGAA GAGTGTTGAA AGCAGCTGGG GAAGAGCTGG AGAAAGGACA GCACTTGGGT 
TCTTCTTCCA AAAAGGAAAG AGACAGACAA AAGCAGAAAA GTATGTCTCT TTTGAGTCAA 
CAACCATTCC TCTCACTGGT CCTTACCTGC CTTAAGGGAC AAGATGAACA ACGAGAAGGC 
CTTCTAACAT CTCTGCAGAA TCAAGTTAAC CAGATTTTAA GTAACTGGAG AGAAGAACGA 
TACCAGGATG ACATCAAAGC ACGACAGATG ATGCATGAGG CATTGCAACT CCGCCTGAAT 
TTGGTGGGAG GAATGTTTGA CACAGTGCAG AGGAGCACCC AGTGGACAAC AGACTGGGCG 
CTTCTCCTCC TTCAGATCAT TACTTCTGGA ACCGTTGACA TGCACACCAA CAATGAATTA 
TTCACAACAG TTCTTGACAT GCTGGGTGTT TTAATCAATG GAACATTGGC CTCTGACCTG 
TCGAATGCAT CCCCAGGGGG ATCTGAAGAG AACAAACGTG CATACATGAA CTTAGTCAAG 
AAACTGAAAA AAGAGCTGGG GGACAAGCGG TCAGAAAGTA TTGACAAAGT TCGACAGCTG 
TTACCTTTGC CGAAACAGAC ATGTGACATC ATTACCTGTG AACCTATGGG CTCCTTGATT 
GACACAAAGG GAAACAAAAT TGCTGGCTTC GATTCTATAG ATAAAAAACA GGGTCTCCAG 
GTCTCCACGA AGCAGAAGGT GTCCCCGTGG GATTTGTTTG AGGGTCAGAA GAACCCGGCT 
CCTCTGTCCT GGGCCTGGTT CGGGACTGTC CGAGTGGACC GGAAGGTGAT CAAGTATGAG 
GAGCAACATC ACCTCCTCCT GTATCACACG CACCCCACGC CCAAGCCCCG GAGTTACTAC 
CTCGAGCCAC TGCCCCTGCC TCCCGAAGAG GAAGAGGAAG AGCCCACATC TCCCATCTCT 
CAGGAACCTG AGAGGAAATC AGCTGAGCTG TCAGATCAGG GAAAAACCGC AACAGATGAA 
GAGAAGAAGA CAAAGGGAAG GAAGCGCAAG ACAAAGTCCA GCTCAAGGGT TGATGAGTAT 
CCGCAGAGCA GCATGTACCG AGTGCCTCCC AACTACTCAC CCATCTCCTC CCAGGTGATG 
CACCACCCGC AGTCCGCCCT GTGGGGCTAC AACCTCATGG GTCAGCCCCA GCAGCCCGGC 
TTCTTCCTTC AGAACCAGTC TGTCACTCCA GGTGGTTCCC GGTTGGACCC CGCTGGCTCC 
TTCGTCCCCA CCAACACCAA GCAAGCTTTG TCCAACATGC TCCAGCGGCG CTCGGGGGCC 
ATGATGCAGC CGCCCTCTCT GCATGCCATC ACATCGCAGC AGCAGTTGAT ACAGATGAAG 
CTTCTGCAGC AGCAGCAGCA ACAGCGGCTT CTCAGGCAAG CCCAGACGCG GCCTTTCCAA 
CAGGGCCAGC CAGGGGACCA GGCTGCTCTC TTTGCTGCTC AGGCACGGCC CTCCCCTCAG 
CTCCCCCAGT ATCCAGGACT GCAGCAAGCA CAGACCATGC CCCAGGGCTA CACAATGTAT 
GGAACCCAGA TGCCTTTGCA GCAGACCCCT CAGCAGCAGG CTGGCAGCGT GGTCCTGTCT 
CCTACCTATA ATTCCAGAAC CTATCCGGCC GCTCACTCCA ACCCAGCGCT CATGGAAAGA 
CTCAGACAGA TGCAGCAGCC GCCGAGCGGC TATGTTCAGC AGCAGGCGTC ACCATACCTG 
CATCCCCTGA CCGGCTCCCA GAGGCTGAAT CATCAGTCGC TTCAGCAGAG CCCTCTGGTG 
GGTGGAGGAA TCGATGCGGT GCTGACTTCC GCACATCCAA ACCTTCCCTC AGTGCCCTTG 
TCTCAGGACC CGATGAGACC CAGACAGCCA CAGGTTCGAC AGCAGCAGAG GCTTCTCCAG 
ATGCAGCAGC CCCAGCAGCC CCCCCAGCCC CAGCAGCCTC CCCAGCCTCA GGGGTCCTCG 
CAGACCCAGA GTCAGACCCT TGGTCTCCAA ACAATGCAGC CCCAGCAGCC TCTGTTCCCC 
AGGCAAGGCT TGCAGCAGAC CCAGCAGCAA CAGCAGACGG CTGCCCTGGT GCGGCAGCTC 
CAGAAGCAGC TTTCCAGTAA CCAGCCACAG CAAGGAGTGA CTCCCTACGG GCATCCTTCA 
CACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_010801491.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799793.1 Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X6, mRNA.

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CloneID	OBa54154
Clone ID Related Accession (Same CDS sequence)	XM_010801491.2
Accession Version	XM_010801491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103819.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801491.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGAGA TGTGGTGAAT CAGAAGTCTT AGATGAGAAG 
GAGTCCATTT CCTCAGCCTC CCTCGCTGGA TCTAGTTTAC CTGTTTTCCA GAATGTTCTT 
CTAAGGTTTT TAGATACACA GGCCCCCTCG TTGTCGGATC CAAATAGCGA ATGTGAGAAG 
GTGGAGTTTG TCAACCTGGT GCTGCTCTTC TGTGAGTTCA TCCGGCATGA CGTCTTCTCC 
CACGATGCCT ACATGTGCAC CCTCATCTCT CGGGGAGACC TGTCTGTCAC CGCCTCCACG 
CGACCGCGGT CGCCAGCAGG AGAGAATACG GATGAGCATT ATCCAAAGGA CCACGACATG 
AAAATGGAGG AACAGACTAT TATGGCACAT ATGGGCATTG ACTCAGGAAC TACTAATATT 
TTTGATGAAG TAGACAAGAA TGACTTTAAA ACTGACTTTG GTTCGGAATT TCCAATTTTT 
TCTCCCATGC CTGGAGAGTC CTGTGAGAAT GCCAACCCTT CTCTGGGCAG AAGAATGTCA 
GTTAATGGTG AGAAGCTGTT GAAGAGAGAA AAACCAAGGG AGTTGATTTT TCCATCTAAT 
TATGACCTCC TACGACACCT GCAGTATGCA ACCCATTTTC CCATACCTCT GGATGAATCT 
TCAAGTCATG AATGTAACCA GCGCACAATC CTTCTTTATG GAGTGGGAAA AGAGCGTGAC 
GAAGCCAGAC ATCAGCTGAA GAAGATTACC AAAGATATTT TGAAAATCCT AAATAAGAAG 
AGCAACACAG AGACAGGGGT TGGAGATGAA GGACAGAAAG CCAGGAAGAG CAAACAGGAA 
GCATTTCCGA CCCTGGAGAC TGTGTTCACA AAACTTCAAC TCCTTTCATA TTTTGATCAG 
CATCAAGTGA CATCTCAGAT TTCTAACAAT GTGCTAGAAC AAATCACAAG CTTTGCATCA 
GGAACATCAT ACCATCTCCC ATTGGCTCAC CACATTCAGC TCATCTTTGA TCTCATGGAG 
CCAGCACTTA ATATTAATGG ACTCATTGAC TTCGCAATAC AGCTCCTGAA CGAACTGAGT 
GTTGTGGAGG CAGAACTGCT CCTGAAGTCC TCCAGCCTGG CAGGAAGCTA CACGACAGGA 
CTGTGTGTCT GCATCGTGGC TGTTCTCAGG CGTTACCACA GTTGTCTGAT CCTGAATCCT 
GATCAGACAG CCCAGGTGTT TGAAGGATTG TGTGGTGTGG TAAAGCACGT TGTGAACCCC 
TCAGAGTGCT CTTCCCCCGA AAGATGCATC TTAGCCTACC TCTACGACCT CTACGTGTCA 
TGTAGCCACC TCAGAAGTAA ATTTGGAGAC CTCTTCAGTA GTGCCTGTTC AAAAGTAAAG 
CAAACCATAT ATAACAATGT GATTCCTGCA AATTCTAACT TGCGATGGGA TCCAGACTTC 
ATGATGGATT TTATTGAGAA TCCCTCGGCC CGTAGCGTCA ACTACTCAGT GCTGGGCAAG 
ATCCTTAGTG ACAATGCAGC CAATCGCTAC AGCTTCGTCT GCAACACTCT CATGAATGTA 
TGTATGGGTC ATCAGGATGC TGGAAGGATT AATGACATAG CCAATTTCTC CTCCGAGCTG 
ACAGCCTGCT GCACTGTTCT CAGTTCAGAA TGGCTGGGGG TCCTGAAGGC TCTTTGTTGT 
TCTTCAAATC ATGTGTGGGG CTTTAATGAT GTTCTTTGCA CTGTCGATGT GAGTGACCTT 
TCATTCCATG ATTCATTAGC TACTTTCATT GCTATTCTGA TAGCACGACA GTGTTTCTCC 
CTGGAGGACG TCGTGCAACA CGTCGCACTC CCCTCTCTCC TCGCCGCAGC TTGCGGAGAT 
GCAGATGCAG AGCCTGGGGC AAGAATGACA TGCCGTCTCC TGCTTCATCT CTTTCGAGCT 
CCCCAGGCCT GCCTGTTACC TCAAGCAACT GGCAAACCTT TCCCGGGAAT AAGATCGTCT 
TGTGACAGAC ACCTCTTAGC TGCTGCTCAC AACAGCATTG AAGTGGGAGC CGTGTTTGCT 
GTCTTAAAAG CAATTATGAT GCTTGGAGAT GCCAAAATTG GCAATAACAG TGTCAGCTCT 
TTAAAAAATG ATGACTTCAC CATGAGGGGT TTACGACGTG ATGGGAATGC TGAGGATATC 
TGGACTGCCT CACAAAATTC AAAATCCTGT GGGAAAAGCA TTTCCATAGA AACGGCCAAT 
TTAAGAGAAT ATGCTAGATA TGTACTGAGG ACCATCTGTC AACAGGAATG GGTAGGAGAA 
CATTGTTTAA AAGAACCTGA GAGATTATGC ACAGACAAAG AACTTATTCT GGACCCTGTG 
CTCTCAAATA TGCAAGCACA GAAATTACTG CAGCTGATCT GTTATCCTCA TGGCATTAAG 
GAATGTACGG AGGGGGACAA CCTCCAAAGA CAGCACATTA AACGCATTCT TCAGAATCTT 
GAGCAGTGGA CGCTGAGGCA GTCCTGGCTG GAGCTTCAGT TAATGATCAA ACAGTGCTTG 
AAGGACCCTG GCTCTGGTTC GGTGGCTGAA ATGAACAACC TGCTGGACAA TATTGCCAAG 
GCAACAATAG AGGTGTTCCA GCAGTCCGCT GACCTAAACA ATAACTCTTC TAATGCTGGC 
ATGGGCCTCT TCAACCCAAA TGGAATTGGA AGTACTGATA CAAGTAGCAC GAGACAGAAT 
GGAATAAAGA CATTTCTAAG TTCCTCTGAA CGCAGGGGTG TGTGGCTGGT GGCCCCCCTA 
ATTGCCAGGC TGCCGACTTC TGTGCAGGGA AGAGTGTTGA AAGCAGCTGG GGAAGAGCTG 
GAGAAAGGAC AGCACTTGGG TTCTTCTTCC AAAAAGGAAA GAGACAGACA AAAGCAGAAA 
AGTATGTCTC TTTTGAGTCA ACAACCATTC CTCTCACTGG TCCTTACCTG CCTTAAGGGA 
CAAGATGAAC AACGAGAAGG CCTTCTAACA TCTCTGCAGA ATCAAGTTAA CCAGATTTTA 
AGTAACTGGA GAGAAGAACG ATACCAGGAT GACATCAAAG CACGACAGAT GATGCATGAG 
GCATTGCAAC TCCGCCTGAA TTTGGTGGGA GGAATGTTTG ACACAGTGCA GAGGAGCACC 
CAGTGGACAA CAGACTGGGC GCTTCTCCTC CTTCAGATCA TTACTTCTGG AACCGTTGAC 
ATGCACACCA ACAATGAATT ATTCACAACA GTTCTTGACA TGCTGGGTGT TTTAATCAAT 
GGAACATTGG CCTCTGACCT GTCGAATGCA TCCCCAGGGG GATCTGAAGA GAACAAACGT 
GCATACATGA ACTTAGTCAA GAAACTGAAA AAAGAGCTGG GGGACAAGCG GTCAGAAAGT 
ATTGACAAAG TTCGACAGCT GTTACCTTTG CCGAAACAGA CATGTGACAT CATTACCTGT 
GAACCTATGG GCTCCTTGAT TGACACAAAG GGAAACAAAA TTGCTGGCTT CGATTCTATA 
GATAAAAAAC AGGGTCTCCA GGTCTCCACG AAGCAGAAGG TGTCCCCGTG GGATTTGTTT 
GAGGGTCAGA AGAACCCGGC TCCTCTGTCC TGGGCCTGGT TCGGGACTGT CCGAGTGGAC 
CGGAAGGTGA TCAAGTATGA GGAGCAACAT CACCTCCTCC TGTATCACAC GCACCCCACG 
CCCAAGCCCC GGAGTTACTA CCTCGAGCCA CTGCCCCTGC CTCCCGAAGA GGAAGAGGAA 
GAGCCCACAT CTCCCATCTC TCAGGAACCT GAGAGGAAAT CAGCTGAGCT GTCAGATCAG 
GGAAAAACCG CAACAGATGA AGAGAAGAAG ACAAAGGGAA GGAAGCGCAA GACAAAGTCC 
AGCTCAAGGG TTGATGAGTA TCCGCAGAGC AGCATGTACC GAGTGCCTCC CAACTACTCA 
CCCATCTCCT CCCAGGTGAT GCACCACCCG CAGTCCGCCC TGTGGGGCTA CAACCTCATG 
GGTCAGCCCC AGCAGCCCGG CTTCTTCCTT CAGAACCAGT CTGTCACTCC AGGTGGTTCC 
CGGTTGGACC CCGCTGGCTC CTTCGTCCCC ACCAACACCA AGCAAGCTTT GTCCAACATG 
CTCCAGCGGC GCTCGGGGGC CATGATGCAG CCGCCCTCTC TGCATGCCAT CACATCGCAG 
CAGCAGTTGA TACAGATGAA GCTTCTGCAG CAGCAGCAGC AACAGCGGCT TCTCAGGCAA 
GCCCAGACGC GGCCTTTCCA ACAGGGCCAG CCAGGGGACC AGGCTGCTCT CTTTGCTGCT 
CAGGCACGGC CCTCCCCTCA GCTCCCCCAG TATCCAGGAC TGCAGCAAGC ACAGACCATG 
CCCCAGGGCT ACACAATGTA TGGAACCCAG ATGCCTTTGC AGCAGACCCC TCAGCAGCAG 
GCTGGCAGCG TGGTCCTGTC TCCTACCTAT AATTCCAGAA CCTATCCGGC CGCTCACTCC 
AACCCAGCGC TCATGGAAAG ACTCAGACAG ATGCAGCAGC CGCCGAGCGG CTATGTTCAG 
CAGCAGGCGT CACCATACCT GCATCCCCTG ACCGGCTCCC AGAGGCTGAA TCATCAGTCG 
CTTCAGCAGA GCCCTCTGGT GGGTGGAGGA ATCGATGCGG TGCTGACTTC CGCACATCCA 
AACCTTCCCT CAGTGCCCTT GTCTCAGGAC CCGATGAGAC CCAGACAGCC ACAGGTTCGA 
CAGCAGCAGA GGCTTCTCCA GATGCAGCAG CCCCAGCAGC CCCCCCAGCC CCAGCAGCCT 
CCCCAGCCTC AGGGGTCCTC GCAGACCCAG AGTCAGACCC TTGGTCTCCA AACAATGCAG 
CCCCAGCAGC CTCTGTTCCC CAGGCAAGGC TTGCAGCAGA CCCAGCAGCA ACAGCAGACG 
GCTGCCCTGG TGCGGCAGCT CCAGAAGCAG CTTTCCAGTA ACCAGCCACA GCAAGGAGTG 
ACTCCCTACG GGCATCCTTC ACACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799793.1
CDS	321..6770
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRPATDPNLEWTQISTRYLREQLAKISDFYHLASSAGDGPVPVPPDVEQAMRQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHVIIGPNSSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPAGENTDEHYPKDHDMKMEEQTIMAHMGIDSGTTNIFDEVDKNDFKTDFGSEFPIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSNTETGVGDEGQKARKSKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVIPANSNLRWDPDFMMDFIENPSARSVNYSVLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLKNDDFTMRGLRRDGNAEDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNAGMGLFNPNGIGSTDTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDIITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPTPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSSMYRVPPNYSPISSQVMHHPQSALWGYNLMGQPQQPGFFLQNQSVTPGGSRLDPAGSFVPTNTKQALSNMLQRRSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGSVVLSPTYNSRTYPAAHSNPALMERLRQMQQPPSGYVQQQASPYLHPLTGSQRLNHQSLQQSPLVGGGIDAVLTSAHPNLPSVPLSQDPMRPRQPQVRQQQRLLQMQQPQQPPQPQQPPQPQGSSQTQSQTLGLQTMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_010801491.2
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator complex subunit 12 like (MED12L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801491.2

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCAGATG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC CGCAGTGAAT 
GTAAAGCAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCTAGA 
AATATTGTGA TTAACCCATC AAAGATCGGA GCTTATTTTA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTATTGGC TGGTTACTGC TCGATCCCAG AGTGCGATTC ATAGCTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTCCCTCAGG TTCCCATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCGAAGTAT 
TCAGTGCCAA TGGTTCGAGC AACATGGCTG ATCAAGATGA CGTGTGCCTA TTACTCTGCT 
ATTTCTGAAG CGAAAATTAA GAAACGTCCG GCCACTGATC CCAATTTGGA GTGGACGCAA 
ATATCTACCA GGTATCTTCG AGAGCAGTTG GCCAAGATTT CCGACTTCTA CCACCTGGCT 
TCCAGCGCGG GCGATGGCCC TGTCCCTGTG CCACCCGACG TGGAGCAGGC CATGAGGCAG 
TGGGAGTACA ACGAAAAGTT GGCATTCCAC ATGTTCCAGG AAGGAATGCT GGAAAAGCAT 
GAATATTTGA CATGGATATT GGATGTGTTA GAAAAGATCA GACCAATGGA TGATGATCTT 
CTTAAACTCT TATTACCACT AATGCTGCAG TATTCAGATG AGTTTGTCCA GTCGGCCTAC 
CTGTCCCGTC GTCTCGCCTA TTTCTGTGCC CGGCGTCTGG CGTTGCTGCT GAGCGACAGC 
CCCAGCCTCC TTGCCGCCCA CTCGCCCCAT GTGATAATAG GCCCAAACAG CTCGAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GGCATGAGCC CTGTGCAGCT GGCCTTCTCC 
GACTTTCTTT CCTGTGCACA GCACGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTTACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ACTCCACAAA CGACAATAAG 
AGCGCGAACC CGGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG CCTCCCCATG 
CCGGGAGGAA ACACGGCTTT CAACCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCGACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAGGTATT GGATCGACAC 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGACAA CCAAGAGAGA TGTGGTGAAT CAGAAGTCTT AGATGAGAAG 
GAGTCCATTT CCTCAGCCTC CCTCGCTGGA TCTAGTTTAC CTGTTTTCCA GAATGTTCTT 
CTAAGGTTTT TAGATACACA GGCCCCCTCG TTGTCGGATC CAAATAGCGA ATGTGAGAAG 
GTGGAGTTTG TCAACCTGGT GCTGCTCTTC TGTGAGTTCA TCCGGCATGA CGTCTTCTCC 
CACGATGCCT ACATGTGCAC CCTCATCTCT CGGGGAGACC TGTCTGTCAC CGCCTCCACG 
CGACCGCGGT CGCCAGCAGG AGAGAATACG GATGAGCATT ATCCAAAGGA CCACGACATG 
AAAATGGAGG AACAGACTAT TATGGCACAT ATGGGCATTG ACTCAGGAAC TACTAATATT 
TTTGATGAAG TAGACAAGAA TGACTTTAAA ACTGACTTTG GTTCGGAATT TCCAATTTTT 
TCTCCCATGC CTGGAGAGTC CTGTGAGAAT GCCAACCCTT CTCTGGGCAG AAGAATGTCA 
GTTAATGGTG AGAAGCTGTT GAAGAGAGAA AAACCAAGGG AGTTGATTTT TCCATCTAAT 
TATGACCTCC TACGACACCT GCAGTATGCA ACCCATTTTC CCATACCTCT GGATGAATCT 
TCAAGTCATG AATGTAACCA GCGCACAATC CTTCTTTATG GAGTGGGAAA AGAGCGTGAC 
GAAGCCAGAC ATCAGCTGAA GAAGATTACC AAAGATATTT TGAAAATCCT AAATAAGAAG 
AGCAACACAG AGACAGGGGT TGGAGATGAA GGACAGAAAG CCAGGAAGAG CAAACAGGAA 
GCATTTCCGA CCCTGGAGAC TGTGTTCACA AAACTTCAAC TCCTTTCATA TTTTGATCAG 
CATCAAGTGA CATCTCAGAT TTCTAACAAT GTGCTAGAAC AAATCACAAG CTTTGCATCA 
GGAACATCAT ACCATCTCCC ATTGGCTCAC CACATTCAGC TCATCTTTGA TCTCATGGAG 
CCAGCACTTA ATATTAATGG ACTCATTGAC TTCGCAATAC AGCTCCTGAA CGAACTGAGT 
GTTGTGGAGG CAGAACTGCT CCTGAAGTCC TCCAGCCTGG CAGGAAGCTA CACGACAGGA 
CTGTGTGTCT GCATCGTGGC TGTTCTCAGG CGTTACCACA GTTGTCTGAT CCTGAATCCT 
GATCAGACAG CCCAGGTGTT TGAAGGATTG TGTGGTGTGG TAAAGCACGT TGTGAACCCC 
TCAGAGTGCT CTTCCCCCGA AAGATGCATC TTAGCCTACC TCTACGACCT CTACGTGTCA 
TGTAGCCACC TCAGAAGTAA ATTTGGAGAC CTCTTCAGTA GTGCCTGTTC AAAAGTAAAG 
CAAACCATAT ATAACAATGT GATTCCTGCA AATTCTAACT TGCGATGGGA TCCAGACTTC 
ATGATGGATT TTATTGAGAA TCCCTCGGCC CGTAGCGTCA ACTACTCAGT GCTGGGCAAG 
ATCCTTAGTG ACAATGCAGC CAATCGCTAC AGCTTCGTCT GCAACACTCT CATGAATGTA 
TGTATGGGTC ATCAGGATGC TGGAAGGATT AATGACATAG CCAATTTCTC CTCCGAGCTG 
ACAGCCTGCT GCACTGTTCT CAGTTCAGAA TGGCTGGGGG TCCTGAAGGC TCTTTGTTGT 
TCTTCAAATC ATGTGTGGGG CTTTAATGAT GTTCTTTGCA CTGTCGATGT GAGTGACCTT 
TCATTCCATG ATTCATTAGC TACTTTCATT GCTATTCTGA TAGCACGACA GTGTTTCTCC 
CTGGAGGACG TCGTGCAACA CGTCGCACTC CCCTCTCTCC TCGCCGCAGC TTGCGGAGAT 
GCAGATGCAG AGCCTGGGGC AAGAATGACA TGCCGTCTCC TGCTTCATCT CTTTCGAGCT 
CCCCAGGCCT GCCTGTTACC TCAAGCAACT GGCAAACCTT TCCCGGGAAT AAGATCGTCT 
TGTGACAGAC ACCTCTTAGC TGCTGCTCAC AACAGCATTG AAGTGGGAGC CGTGTTTGCT 
GTCTTAAAAG CAATTATGAT GCTTGGAGAT GCCAAAATTG GCAATAACAG TGTCAGCTCT 
TTAAAAAATG ATGACTTCAC CATGAGGGGT TTACGACGTG ATGGGAATGC TGAGGATATC 
TGGACTGCCT CACAAAATTC AAAATCCTGT GGGAAAAGCA TTTCCATAGA AACGGCCAAT 
TTAAGAGAAT ATGCTAGATA TGTACTGAGG ACCATCTGTC AACAGGAATG GGTAGGAGAA 
CATTGTTTAA AAGAACCTGA GAGATTATGC ACAGACAAAG AACTTATTCT GGACCCTGTG 
CTCTCAAATA TGCAAGCACA GAAATTACTG CAGCTGATCT GTTATCCTCA TGGCATTAAG 
GAATGTACGG AGGGGGACAA CCTCCAAAGA CAGCACATTA AACGCATTCT TCAGAATCTT 
GAGCAGTGGA CGCTGAGGCA GTCCTGGCTG GAGCTTCAGT TAATGATCAA ACAGTGCTTG 
AAGGACCCTG GCTCTGGTTC GGTGGCTGAA ATGAACAACC TGCTGGACAA TATTGCCAAG 
GCAACAATAG AGGTGTTCCA GCAGTCCGCT GACCTAAACA ATAACTCTTC TAATGCTGGC 
ATGGGCCTCT TCAACCCAAA TGGAATTGGA AGTACTGATA CAAGTAGCAC GAGACAGAAT 
GGAATAAAGA CATTTCTAAG TTCCTCTGAA CGCAGGGGTG TGTGGCTGGT GGCCCCCCTA 
ATTGCCAGGC TGCCGACTTC TGTGCAGGGA AGAGTGTTGA AAGCAGCTGG GGAAGAGCTG 
GAGAAAGGAC AGCACTTGGG TTCTTCTTCC AAAAAGGAAA GAGACAGACA AAAGCAGAAA 
AGTATGTCTC TTTTGAGTCA ACAACCATTC CTCTCACTGG TCCTTACCTG CCTTAAGGGA 
CAAGATGAAC AACGAGAAGG CCTTCTAACA TCTCTGCAGA ATCAAGTTAA CCAGATTTTA 
AGTAACTGGA GAGAAGAACG ATACCAGGAT GACATCAAAG CACGACAGAT GATGCATGAG 
GCATTGCAAC TCCGCCTGAA TTTGGTGGGA GGAATGTTTG ACACAGTGCA GAGGAGCACC 
CAGTGGACAA CAGACTGGGC GCTTCTCCTC CTTCAGATCA TTACTTCTGG AACCGTTGAC 
ATGCACACCA ACAATGAATT ATTCACAACA GTTCTTGACA TGCTGGGTGT TTTAATCAAT 
GGAACATTGG CCTCTGACCT GTCGAATGCA TCCCCAGGGG GATCTGAAGA GAACAAACGT 
GCATACATGA ACTTAGTCAA GAAACTGAAA AAAGAGCTGG GGGACAAGCG GTCAGAAAGT 
ATTGACAAAG TTCGACAGCT GTTACCTTTG CCGAAACAGA CATGTGACAT CATTACCTGT 
GAACCTATGG GCTCCTTGAT TGACACAAAG GGAAACAAAA TTGCTGGCTT CGATTCTATA 
GATAAAAAAC AGGGTCTCCA GGTCTCCACG AAGCAGAAGG TGTCCCCGTG GGATTTGTTT 
GAGGGTCAGA AGAACCCGGC TCCTCTGTCC TGGGCCTGGT TCGGGACTGT CCGAGTGGAC 
CGGAAGGTGA TCAAGTATGA GGAGCAACAT CACCTCCTCC TGTATCACAC GCACCCCACG 
CCCAAGCCCC GGAGTTACTA CCTCGAGCCA CTGCCCCTGC CTCCCGAAGA GGAAGAGGAA 
GAGCCCACAT CTCCCATCTC TCAGGAACCT GAGAGGAAAT CAGCTGAGCT GTCAGATCAG 
GGAAAAACCG CAACAGATGA AGAGAAGAAG ACAAAGGGAA GGAAGCGCAA GACAAAGTCC 
AGCTCAAGGG TTGATGAGTA TCCGCAGAGC AGCATGTACC GAGTGCCTCC CAACTACTCA 
CCCATCTCCT CCCAGGTGAT GCACCACCCG CAGTCCGCCC TGTGGGGCTA CAACCTCATG 
GGTCAGCCCC AGCAGCCCGG CTTCTTCCTT CAGAACCAGT CTGTCACTCC AGGTGGTTCC 
CGGTTGGACC CCGCTGGCTC CTTCGTCCCC ACCAACACCA AGCAAGCTTT GTCCAACATG 
CTCCAGCGGC GCTCGGGGGC CATGATGCAG CCGCCCTCTC TGCATGCCAT CACATCGCAG 
CAGCAGTTGA TACAGATGAA GCTTCTGCAG CAGCAGCAGC AACAGCGGCT TCTCAGGCAA 
GCCCAGACGC GGCCTTTCCA ACAGGGCCAG CCAGGGGACC AGGCTGCTCT CTTTGCTGCT 
CAGGCACGGC CCTCCCCTCA GCTCCCCCAG TATCCAGGAC TGCAGCAAGC ACAGACCATG 
CCCCAGGGCT ACACAATGTA TGGAACCCAG ATGCCTTTGC AGCAGACCCC TCAGCAGCAG 
GCTGGCAGCG TGGTCCTGTC TCCTACCTAT AATTCCAGAA CCTATCCGGC CGCTCACTCC 
AACCCAGCGC TCATGGAAAG ACTCAGACAG ATGCAGCAGC CGCCGAGCGG CTATGTTCAG 
CAGCAGGCGT CACCATACCT GCATCCCCTG ACCGGCTCCC AGAGGCTGAA TCATCAGTCG 
CTTCAGCAGA GCCCTCTGGT GGGTGGAGGA ATCGATGCGG TGCTGACTTC CGCACATCCA 
AACCTTCCCT CAGTGCCCTT GTCTCAGGAC CCGATGAGAC CCAGACAGCC ACAGGTTCGA 
CAGCAGCAGA GGCTTCTCCA GATGCAGCAG CCCCAGCAGC CCCCCCAGCC CCAGCAGCCT 
CCCCAGCCTC AGGGGTCCTC GCAGACCCAG AGTCAGACCC TTGGTCTCCA AACAATGCAG 
CCCCAGCAGC CTCTGTTCCC CAGGCAAGGC TTGCAGCAGA CCCAGCAGCA ACAGCAGACG 
GCTGCCCTGG TGCGGCAGCT CCAGAAGCAG CTTTCCAGTA ACCAGCCACA GCAAGGAGTG 
ACTCCCTACG GGCATCCTTC ACACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MED12L
Entrez Gene ID	538979
Full Name	mediator complex subunit 12 like
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	MED12L	NP_443728.3
Pan troglodytes (chimpanzee)	MED12L	XP_516821.2
Mus musculus (house mouse)	Med12l	NP_808523.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002523	XP_312414.5
Drosophila melanogaster (fruit fly)	kto	NP_524786.1
	MED12L	XP_001107022.2
Gallus gallus (chicken)	MED12L	XP_422838.4
Canis lupus familiaris (dog)	MED12L	XP_005634712.1
Bos taurus (cattle)	MED12L	NP_001193434.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MED12L cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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