ZNF521 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZNF521
Entrez Gene ID	538792
Full Name	zinc finger protein 521
General protein information	Preferred Names zinc finger protein 521 Names zinc finger protein 521
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015460072.1	XP_015315558.1	zinc finger protein 521 isoform X3
XM_010818763.2	XP_010817065.1	zinc finger protein 521 isoform X2
XM_015460072.2	XP_015315558.1	zinc finger protein 521 isoform X3
XM_005224070.4	XP_005224127.1	zinc finger protein 521 isoform X1
XM_005224070.3	XP_005224127.1	zinc finger protein 521 isoform X1
XM_010818763.3	XP_010817065.1	zinc finger protein 521 isoform X2
XM_010818761.2	XP_010817063.1	zinc finger protein 521 isoform X1
NM_001105419.1	NP_001098889.1	zinc finger protein 521

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ZNF521	NP_001098889.1
Mus musculus (house mouse)	Zfp521	NP_663467.1
Pan troglodytes (chimpanzee)	ZNF521	XP_512068.2
Rattus norvegicus (Norway rat)	Zfp521	NP_001100873.1
Canis lupus familiaris (dog)	ZNF521	XP_547633.2
Gallus gallus (chicken)	ZNF521	XP_004939860.1
Xenopus tropicalis (tropical clawed frog)	znf521	XP_004915266.1
Homo sapiens (human)	ZNF521	NP_056276.1
	LOC709169	XP_002800935.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ZNF521 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZNF521 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa88303	XM_015460072.2 Latest version!	Bos taurus zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa39759	NM_001105419.1 Latest version!	Bos taurus zinc finger protein 521 (ZNF521), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa32463	XM_005224070.4 Latest version!	Bos taurus zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa32463	XM_010818761.2 Latest version!	Bos taurus zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa54067	XM_010818763.3 Latest version!	Bos taurus zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OBa88303	XM_015460072.1	Bos taurus zinc finger protein 521 (ZNF521), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa32463	XM_005224070.3	Bos taurus zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa54067	XM_010818763.2	Bos taurus zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZNF521 ( XM_015460072.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315558.1 Bos taurus zinc finger protein 521 (ZNF521), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa88303
Clone ID Related Accession (Same CDS sequence)	XM_015460072.1 , XM_015460072.2
Accession Version	XM_015460072.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	zinc finger protein 521 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCA GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCACG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TTGACTCCCC TGCCAAGCTG 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315558.1
CDS	807..4082
Translation	MQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCVYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDGHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKASAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_015460072.1
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger protein 521 (ZNF521), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460072.1

ORF Insert Sequence:

ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCA GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCACG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TTGACTCCCC TGCCAAGCTG 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_015460072.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315558.1 Bos taurus zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa88303
Clone ID Related Accession (Same CDS sequence)	XM_015460072.1 , XM_015460072.2
Accession Version	XM_015460072.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	zinc finger protein 521 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460072.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCA GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCACG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TTGACTCCCC TGCCAAGCTG 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315558.1
CDS	828..4103
Translation	MQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCVYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDGHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKASAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_015460072.2
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460072.2

ORF Insert Sequence:

ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCA GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCACG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TTGACTCCCC TGCCAAGCTG 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( NM_001105419.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001098889.1 Bos taurus zinc finger protein 521 (ZNF521), mRNA.

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CloneID	OBa39759
Clone ID Related Accession (Same CDS sequence)	NM_001105419.1
Accession Version	NM_001105419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	zinc finger protein 521
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC153228.1. On Sep 25, 2007 this sequence version replaced XM_582772.3. ##Evidence-Data-START## Transcript exon combination :: BC153228.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGTCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCTT CTAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCG GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCACG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TTGACTCCCC TGCCAAGCTG 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001098889.1
CDS	8..3943
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCDSCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCVYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDGHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKAGAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	NM_001105419.1
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger protein 521 (ZNF521), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105419.1

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGTCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCTT CTAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCG GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCACG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TTGACTCCCC TGCCAAGCTG 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_005224070.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224127.1 Bos taurus zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32463
Clone ID Related Accession (Same CDS sequence)	XM_005224070.4 , XM_005224070.3 , XM_010818761.2
Accession Version	XM_005224070.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	zinc finger protein 521 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224070.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGTCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCTT CTAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCA GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCACG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TTGACTCCCC TGCCAAGCTG 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224127.1
CDS	168..4103
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCDSCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCVYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDGHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKASAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_005224070.4
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224070.4

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGTCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCTT CTAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCA GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCACG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TTGACTCCCC TGCCAAGCTG 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_005224070.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224127.1 Bos taurus zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32463
Clone ID Related Accession (Same CDS sequence)	XM_005224070.4 , XM_005224070.3 , XM_010818761.2
Accession Version	XM_005224070.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	zinc finger protein 521 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104567.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224070.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGTCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCTT CTAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCA GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCACG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TTGACTCCCC TGCCAAGCTG 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224127.1
CDS	104..4039
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCDSCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCVYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDGHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKASAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_005224070.3
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224070.3

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGTCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCTT CTAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCA GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCACG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TTGACTCCCC TGCCAAGCTG 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_010818761.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817063.1 Bos taurus zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.

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CloneID	OBa32463
Clone ID Related Accession (Same CDS sequence)	XM_005224070.4 , XM_005224070.3 , XM_010818761.2
Accession Version	XM_010818761.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	zinc finger protein 521 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104567.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818761.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGTCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCTT CTAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCA GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCACG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TTGACTCCCC TGCCAAGCTG 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817063.1
CDS	191..4126
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCDSCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCVYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDGHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKASAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_010818761.2
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818761.2

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGTCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCTT CTAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCA GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCACG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TTGACTCCCC TGCCAAGCTG 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_010818763.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817065.1 Bos taurus zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa54067
Clone ID Related Accession (Same CDS sequence)	XM_010818763.2 , XM_010818763.3
Accession Version	XM_010818763.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	zinc finger protein 521 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818763.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGTCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCTT CTAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCA GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGACCT ATCAGTGCAT CAAGTGTCAG 
ATGGTTTTCT ACAATGAATG GGATATTCAG GTGCACGTTG CAAATCACAT GATTGATGAA 
GGGCTGAACC ACGAGTGCAA ACTTTGCAGC CAAACCTTTG ACTCCCCTGC CAAGCTGCAG 
TGCCACCTGA TCGAGCACAG CTTCGAAGGG ATGGGCGGTA CCTTCAAGTG TCCCGTCTGC 
TTTACAGTGT TTGTTCAAGC AAACAAGTTG CAGCAGCATA TTTTCTCTGC CCATGGACAA 
GAAGACAAGA TCTACGACTG CACACAATGT CCACAGAAGT TTTTCTTCCA AACAGAGCTG 
CAGAACCATA CGATGACCCA ACACAGCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817065.1
CDS	104..4036
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCDSCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCVYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDGHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKASAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_010818763.2
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818763.2

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGTCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCTT CTAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCA GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGACCT ATCAGTGCAT CAAGTGTCAG 
ATGGTTTTCT ACAATGAATG GGATATTCAG GTGCACGTTG CAAATCACAT GATTGATGAA 
GGGCTGAACC ACGAGTGCAA ACTTTGCAGC CAAACCTTTG ACTCCCCTGC CAAGCTGCAG 
TGCCACCTGA TCGAGCACAG CTTCGAAGGG ATGGGCGGTA CCTTCAAGTG TCCCGTCTGC 
TTTACAGTGT TTGTTCAAGC AAACAAGTTG CAGCAGCATA TTTTCTCTGC CCATGGACAA 
GAAGACAAGA TCTACGACTG CACACAATGT CCACAGAAGT TTTTCTTCCA AACAGAGCTG 
CAGAACCATA CGATGACCCA ACACAGCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_010818763.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817065.1 Bos taurus zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa54067
Clone ID Related Accession (Same CDS sequence)	XM_010818763.2 , XM_010818763.3
Accession Version	XM_010818763.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	zinc finger protein 521 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818763.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGTCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCTT CTAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCA GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGACCT ATCAGTGCAT CAAGTGTCAG 
ATGGTTTTCT ACAATGAATG GGATATTCAG GTGCACGTTG CAAATCACAT GATTGATGAA 
GGGCTGAACC ACGAGTGCAA ACTTTGCAGC CAAACCTTTG ACTCCCCTGC CAAGCTGCAG 
TGCCACCTGA TCGAGCACAG CTTCGAAGGG ATGGGCGGTA CCTTCAAGTG TCCCGTCTGC 
TTTACAGTGT TTGTTCAAGC AAACAAGTTG CAGCAGCATA TTTTCTCTGC CCATGGACAA 
GAAGACAAGA TCTACGACTG CACACAATGT CCACAGAAGT TTTTCTTCCA AACAGAGCTG 
CAGAACCATA CGATGACCCA ACACAGCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817065.1
CDS	132..4064
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCDSCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCVYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDGHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKASAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_010818763.3
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818763.3

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGTCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCTT CTAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAA CAAGGACGGC TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGACTT CCCAGAAGAC 
CTCCAAAAGC ACATCGCTGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGTG TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CGTCCCTGAT GAACCACATG 
GAGCAGATGC ACGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC GGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCTTGGT CACCGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA TGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTCC CCAACCAAGA GTCCCTGCTG 
AAGCACGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CGTCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGATTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACAGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CCGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGCG TGTTCGACAC CAAGACGCCC 
AACTGTGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCAGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATCGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCCGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACTT TGACTGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCGCAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGTG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCA GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCGCA AGTCTCGCCC 
ATGCCCAGAA TCAGCCCCTC GCAATCGGAT GAGAAGACCT ATCAGTGCAT CAAGTGTCAG 
ATGGTTTTCT ACAATGAATG GGATATTCAG GTGCACGTTG CAAATCACAT GATTGATGAA 
GGGCTGAACC ACGAGTGCAA ACTTTGCAGC CAAACCTTTG ACTCCCCTGC CAAGCTGCAG 
TGCCACCTGA TCGAGCACAG CTTCGAAGGG ATGGGCGGTA CCTTCAAGTG TCCCGTCTGC 
TTTACAGTGT TTGTTCAAGC AAACAAGTTG CAGCAGCATA TTTTCTCTGC CCATGGACAA 
GAAGACAAGA TCTACGACTG CACACAATGT CCACAGAAGT TTTTCTTCCA AACAGAGCTG 
CAGAACCATA CGATGACCCA ACACAGCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ZNF521 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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