DARS2 cDNA ORF clone, Bos taurus(cattle)

The following DARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa43208 NM_001102222.1
Latest version!
Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa27314 XM_005217103.3
Latest version!
Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa27314 XM_010813347.2
Latest version!
Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa27314 XM_005217104.2
Latest version!
Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa27314 XM_015475153.1
Latest version!
Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa223672 XM_015475153.2
Latest version!
Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa223672 XM_010813347.3
Latest version!
Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa223672 XM_005217103.4
Latest version!
Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa223672 XM_005217104.3
Latest version!
Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa27315 XM_024976366.1
Latest version!
Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa43208
    Clone ID Related Accession (Same CDS sequence) NM_001102222.1
    Accession Version NM_001102222.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Bos taurus(cattle)
    Product aspartate--tRNA ligase, mitochondrial precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC149492.1. On Aug 21, 2007 this sequence version replaced XM_582590.3. ##Evidence-Data-START## Transcript exon combination :: BC149492.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145467, SAMN04019638 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTTCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTGCGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001095692.1
    CDS308..2263
    Translation

    Target ORF information:

    RefSeq Version NM_001102222.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102222.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTTCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTGCGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27314
    Clone ID Related Accession (Same CDS sequence) XM_010813347.2 , XM_005217104.2 , XM_005217103.3 , XM_015475153.1
    Accession Version XM_010813347.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product aspartate--tRNA ligase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813347.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTTCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811649.1
    CDS601..2556
    Translation

    Target ORF information:

    RefSeq Version XM_010813347.2
    Organism Bos taurus(cattle)
    Definition Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813347.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTTCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27314
    Clone ID Related Accession (Same CDS sequence) XM_010813347.2 , XM_005217104.2 , XM_005217103.3 , XM_015475153.1
    Accession Version XM_005217104.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product aspartate--tRNA ligase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005217104.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTTCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217161.1
    CDS170..2125
    Translation

    Target ORF information:

    RefSeq Version XM_005217104.2
    Organism Bos taurus(cattle)
    Definition Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217104.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTTCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27314
    Clone ID Related Accession (Same CDS sequence) XM_010813347.2 , XM_005217104.2 , XM_005217103.3 , XM_015475153.1
    Accession Version XM_005217103.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product aspartate--tRNA ligase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217103.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTTCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217160.1
    CDS166..2121
    Translation

    Target ORF information:

    RefSeq Version XM_005217103.3
    Organism Bos taurus(cattle)
    Definition Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217103.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTTCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27314
    Clone ID Related Accession (Same CDS sequence) XM_010813347.2 , XM_005217104.2 , XM_005217103.3 , XM_015475153.1
    Accession Version XM_015475153.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product aspartate--tRNA ligase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTTCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330639.1
    CDS179..2134
    Translation

    Target ORF information:

    RefSeq Version XM_015475153.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475153.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTTCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223672
    Clone ID Related Accession (Same CDS sequence) XM_005217104.3 , XM_010813347.3 , XM_015475153.2 , XM_005217103.4
    Accession Version XM_005217104.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product aspartate--tRNA ligase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217104.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTCCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217161.1
    CDS178..2133
    Translation

    Target ORF information:

    RefSeq Version XM_005217104.3
    Organism Bos taurus(cattle)
    Definition Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217104.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTCCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223672
    Clone ID Related Accession (Same CDS sequence) XM_005217104.3 , XM_010813347.3 , XM_015475153.2 , XM_005217103.4
    Accession Version XM_010813347.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product aspartate--tRNA ligase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813347.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTCCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811649.1
    CDS608..2563
    Translation

    Target ORF information:

    RefSeq Version XM_010813347.3
    Organism Bos taurus(cattle)
    Definition Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813347.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTCCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223672
    Clone ID Related Accession (Same CDS sequence) XM_005217104.3 , XM_010813347.3 , XM_015475153.2 , XM_005217103.4
    Accession Version XM_015475153.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product aspartate--tRNA ligase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475153.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTCCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330639.1
    CDS186..2141
    Translation

    Target ORF information:

    RefSeq Version XM_015475153.2
    Organism Bos taurus(cattle)
    Definition Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475153.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTCCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223672
    Clone ID Related Accession (Same CDS sequence) XM_005217104.3 , XM_010813347.3 , XM_015475153.2 , XM_005217103.4
    Accession Version XM_005217103.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product aspartate--tRNA ligase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217103.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTCCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217160.1
    CDS177..2132
    Translation

    Target ORF information:

    RefSeq Version XM_005217103.4
    Organism Bos taurus(cattle)
    Definition Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217103.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTCTGTT GGTTAAGTCG ACTGTGCGGG GAATTATCCA CACCCACCAG AAGGACCACC 
    CAGTTGATCT GGAGTTCTGC CGCCCGAAGT ATGGTACTGA GTTCACAGAG AATTCCAGAA
    CTCAGTAGCT TTGTTGCCCG GACCAACACA TGTGGAGAGT TGCGTTCTTC TCACTTAGGC
    CAAGAAGTCA CCTTATGTGG ATGGATTCAG TTCCGAAGGC AAAATATCTT CCTGGTCCTA
    AGAGATTTCC ATGGACTTGT TCAAGTTGTC ATTCCCCAGG ATGAGTCTGC TGCTTCGGTG
    AAGAAGATTT TATGTGAAGC TCCCATGGAA TCTGTGGTGC AAGTGTCTGG GACAGTAATT
    TCCCGACCTC CAGGACAAAA GAATCCAAAA ATGCCAACAG GTGAGATTGA AATCAAAGTT
    AAGACAGCTA AACTTCTGAA TTCCTGCAAG AAGCTGCCCT TTGAAATTAA GGACTTTATG
    AAGAAAACAG AGACTCTTCG TTTGCAGTAC CGCTACTTAG ATTTGCGTAG TGTCCAAATG
    CAGTATAACC TGCGACTGAG ATCCCAGATG GTCATGAAAA TGCGGGAATA TCTCTGTAAT
    CTACATGGTT TTGTGGATGT AGAAACACCA ACATTATTCA AGAGGACTCC AGGGGGTGCA
    AAAGAGTTTG TAATACCATC CAGGGAACCT GGGAAGTTTT ATTCTCTCCC TCAGAGTCCT
    CAACAGTTTA AGCAGCTTCT GATGGTTGGT GGTCTAGACA GATATTTTCA GGTTGCCCGA
    TGTTACCGAG ACGAAGGTTC AAGACCAGAC AGACAGCCTG AATTTACTCA GATCGACATA
    GAGATGTCCT TTGTAGACCA GACGGGCGTC CAGAGTCTAA TTGAGGGCTT GCTCCAGTAT
    TCGTGGCCCA GTGACAAAGA TCCTCTGGTG GTCCCTTTTC CTTCCATGCC TTTTGCTGAG
    GCACTGGCCA GCTATGGAAC CGATAAACCT GACACTCGCT TTGGGATGAA GATTGTAGAT
    ATCAGTGACA TGTTCAGAAA CACAGAAGTT GGATTTCTCC AAGATGCCCT TAGTAAACCC
    CAAGGAACTG TCAAAGCCAT ATGTATCCGT AAAGGAGCAA AATATTTGAA AAGGAAGGAC
    ATTGAATCCA TTAGAAAATT TGCAGCTGAC CATTTTAATG AGGAAGTCTT ACCTATATTC
    CTGAAAACCA ATGAAAACTG GAATTCTCCA GTTGCTAAGT TCATAATGGA GGAGCAAGGC
    TTGGGACTCG TCAAACTGTT GGAGACCCAA GAGGAGGACG TGGTCCTGCT CACTGCCGGA
    GAGCACAAGA AAGCATGCTC TTTGATGGGA AAATTACGAC TGGAGTGTGC TGACCTTCTA
    GAAGCAAGAG GAGTGGTGCT TCGTGACCCC GCTCTGTTCT CTTTCCTTTG GGTGGTGGAT
    TTCCCCCTCT TCCTTCCCAA GGAGGAAAAT CCCCAAGAGC TGGAGTCAGC CCACCACCCA
    TTTACTGCTC CCCATCCGAG TGACATTCAC CTTCTTTACA CTGAGCCCCA CAAGGTCCGT
    AGCCAACACT ATGACTTGGT TTTAAACGGC AATGAGATAG GAGGTGGTTC TATCCGAATT
    CATAATTCAG AGCTTCAGCA TTACGTCCTG GACACAGTAC TAAAGGAAGA TGTGAAATTG
    CTCTCCCATC TGCTCCAGGC TTTAGATTAT GGGGCGCCCC CTCATGGAGG AATTGCCTTA
    GGGTTAGACA GACTGATGTG CCTTGTCACT GGAGCACCAA GCATCAGAGA TGTCATAGCC
    TTCCCCAAAT CCTTCCGGGG GCACGATCTC ATGAGCAATG CCCCAGATTC TATCCCTCCT
    GAGGAACTGA AGCCCTATCA CATCCAGGTC TCCTGGCCAA TGGATGCAGA AACAGAAAAG
    AGCTCATCGA ATCATCCATG CCGTTCAGAA AGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27315
    Clone ID Related Accession (Same CDS sequence) XM_024976366.1
    Accession Version XM_024976366.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product aspartate--tRNA ligase, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGAATCTG TGGTGCAAGT GTCTGGGACA GTAATTTCCC GACCTCCAGG ACAAAAGAAT 
    CCAAAAATGC CAACAGGTGA GATTGAAATC AAAGTTAAGA CAGCTAAACT TCTGAATTCC
    TGCAAGAAGC TGCCCTTTGA AATTAAGGAC TTTATGAAGA AAACAGAGAC TCTTCGTTTG
    CAGTACCGCT ACTTAGATTT GCGTAGTGTC CAAATGCAGT ATAACCTGCG ACTGAGATCC
    CAGATGGTCA TGAAAATGCG GGAATATCTC TGTAATCTAC ATGGTTTTGT GGATGTAGAA
    ACACCAACAT TATTCAAGAG GACTCCAGGG GGTGCAAAAG AGTTTGTAAT ACCATCCAGG
    GAACCTGGGA AGTTTTATTC TCTCCCTCAG AGTCCTCAAC AGTTTAAGCA GCTTCTGATG
    GTTGGTGGTC TAGACAGATA TTTTCAGGTT GCCCGATGTT ACCGAGACGA AGGTTCAAGA
    CCAGACAGAC AGCCTGAATT TACTCAGATC GACATAGAGA TGTCCTTTGT AGACCAGACG
    GGCGTCCAGA GTCTAATTGA GGGCTTGCTC CAGTATTCGT GGCCCAGTGA CAAAGATCCT
    CTGGTGGTCC CTTTTCCTTC CATGCCTTTT GCTGAGGCAC TGGCCAGCTA TGGAACCGAT
    AAACCTGACA CTCGCTTTGG GATGAAGATT GTAGATATCA GTGACATGTT CAGAAACACA
    GAAGTTGGAT TTCTCCAAGA TGCCCTTAGT AAACCCCAAG GAACTGTCAA AGCCATATGT
    ATCCGTAAAG GAGCAAAATA TTTGAAAAGG AAGGACATTG AATCCATTAG AAAATTTGCA
    GCTGACCATT TTAATGAGGA AGTCTTACCT ATATTCCTGA AAACCAATGA AAACTGGAAT
    TCTCCAGTTG CTAAGTTCAT AATGGAGGAG CAAGGCTTGG GACTCGTCAA ACTGTTGGAG
    ACCCAAGAGG AGGACGTGGT CCTGCTCACT GCCGGAGAGC ACAAGAAAGC ATGCTCTTTG
    ATGGGAAAAT TACGACTGGA GTGTGCTGAC CTTCTAGAAG CAAGAGGAGT GGTGCTTCGT
    GACCCCGCTC TGTTCTCTTT CCTTTGGGTG GTGGATTTCC CCCTCTTCCT TCCCAAGGAG
    GAAAATCCCC AAGAGCTGGA GTCAGCCCAC CACCCATTTA CTGCTCCCCA TCCGAGTGAC
    ATTCACCTTC TTTACACTGA GCCCCACAAG GTCCGTAGCC AACACTATGA CTTGGTTTTA
    AACGGCAATG AGATAGGAGG TGGTTCTATC CGAATTCATA ATTCAGAGCT TCAGCATTAC
    GTCCTGGACA CAGTACTAAA GGAAGATGTG AAATTGCTCT CCCATCTGCT CCAGGCTTTA
    GATTATGGGG CGCCCCCTCA TGGAGGAATT GCCTTAGGGT TAGACAGACT GATGTGCCTT
    GTCACTGGAG CACCAAGCAT CAGAGATGTC ATAGCCTTCC CCAAATCCTT CCGGGGGCAC
    GATCTCATGA GCAATGCCCC AGATTCTATC CCTCCTGAGG AACTGAAGCC CTATCACATC
    CAGGTCTCCT GGCCAATGGA TGCAGAAACA GAAAAGAGCT CATCGAATCA TCCATGCCGT
    TCAGAAAGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832134.1
    CDS129..1760
    Translation

    Target ORF information:

    RefSeq Version XM_024976366.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976366.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGAATCTG TGGTGCAAGT GTCTGGGACA GTAATTTCCC GACCTCCAGG ACAAAAGAAT 
    CCAAAAATGC CAACAGGTGA GATTGAAATC AAAGTTAAGA CAGCTAAACT TCTGAATTCC
    TGCAAGAAGC TGCCCTTTGA AATTAAGGAC TTTATGAAGA AAACAGAGAC TCTTCGTTTG
    CAGTACCGCT ACTTAGATTT GCGTAGTGTC CAAATGCAGT ATAACCTGCG ACTGAGATCC
    CAGATGGTCA TGAAAATGCG GGAATATCTC TGTAATCTAC ATGGTTTTGT GGATGTAGAA
    ACACCAACAT TATTCAAGAG GACTCCAGGG GGTGCAAAAG AGTTTGTAAT ACCATCCAGG
    GAACCTGGGA AGTTTTATTC TCTCCCTCAG AGTCCTCAAC AGTTTAAGCA GCTTCTGATG
    GTTGGTGGTC TAGACAGATA TTTTCAGGTT GCCCGATGTT ACCGAGACGA AGGTTCAAGA
    CCAGACAGAC AGCCTGAATT TACTCAGATC GACATAGAGA TGTCCTTTGT AGACCAGACG
    GGCGTCCAGA GTCTAATTGA GGGCTTGCTC CAGTATTCGT GGCCCAGTGA CAAAGATCCT
    CTGGTGGTCC CTTTTCCTTC CATGCCTTTT GCTGAGGCAC TGGCCAGCTA TGGAACCGAT
    AAACCTGACA CTCGCTTTGG GATGAAGATT GTAGATATCA GTGACATGTT CAGAAACACA
    GAAGTTGGAT TTCTCCAAGA TGCCCTTAGT AAACCCCAAG GAACTGTCAA AGCCATATGT
    ATCCGTAAAG GAGCAAAATA TTTGAAAAGG AAGGACATTG AATCCATTAG AAAATTTGCA
    GCTGACCATT TTAATGAGGA AGTCTTACCT ATATTCCTGA AAACCAATGA AAACTGGAAT
    TCTCCAGTTG CTAAGTTCAT AATGGAGGAG CAAGGCTTGG GACTCGTCAA ACTGTTGGAG
    ACCCAAGAGG AGGACGTGGT CCTGCTCACT GCCGGAGAGC ACAAGAAAGC ATGCTCTTTG
    ATGGGAAAAT TACGACTGGA GTGTGCTGAC CTTCTAGAAG CAAGAGGAGT GGTGCTTCGT
    GACCCCGCTC TGTTCTCTTT CCTTTGGGTG GTGGATTTCC CCCTCTTCCT TCCCAAGGAG
    GAAAATCCCC AAGAGCTGGA GTCAGCCCAC CACCCATTTA CTGCTCCCCA TCCGAGTGAC
    ATTCACCTTC TTTACACTGA GCCCCACAAG GTCCGTAGCC AACACTATGA CTTGGTTTTA
    AACGGCAATG AGATAGGAGG TGGTTCTATC CGAATTCATA ATTCAGAGCT TCAGCATTAC
    GTCCTGGACA CAGTACTAAA GGAAGATGTG AAATTGCTCT CCCATCTGCT CCAGGCTTTA
    GATTATGGGG CGCCCCCTCA TGGAGGAATT GCCTTAGGGT TAGACAGACT GATGTGCCTT
    GTCACTGGAG CACCAAGCAT CAGAGATGTC ATAGCCTTCC CCAAATCCTT CCGGGGGCAC
    GATCTCATGA GCAATGCCCC AGATTCTATC CCTCCTGAGG AACTGAAGCC CTATCACATC
    CAGGTCTCCT GGCCAATGGA TGCAGAAACA GAAAAGAGCT CATCGAATCA TCCATGCCGT
    TCAGAAAGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.