NLRP3 cDNA ORF clone, Bos taurus(cattle)

The following NLRP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa54009 XM_015472167.1
Latest version!
Bos taurus NLR family, pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa54010 XM_015472168.1
Latest version!
Bos taurus NLR family, pyrin domain containing 3 (NLRP3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa236153 XM_015472167.2
Latest version!
Bos taurus NLR family pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa236154 XM_015472168.2
Latest version!
Bos taurus NLR family pyrin domain containing 3 (NLRP3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa236155 XM_024994442.1
Latest version!
Bos taurus NLR family pyrin domain containing 3 (NLRP3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa236152 XM_010807177.3
Latest version!
Bos taurus NLR family pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa21365 NM_001102219.1
Latest version!
Bos taurus NLR family pyrin domain containing 3 (NLRP3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa21365 XM_010807177.2
Latest version!
Bos taurus NLR family, pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa54009
    Clone ID Related Accession (Same CDS sequence) XM_015472167.1
    Accession Version XM_015472167.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2925bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104026.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGGG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGTTGGTG AATTCTGGCC TTACATCAGG TTGTTGTCCA
    GCTCTATCCT CAGTGCTCAG TACAAATCAG AATCTCACCC ACCTTTACCT ACAGGGCAAT
    GCTCTTGGAG ACATGGGGGT CAAGCTACTC TGTGAGGGAC TCTTGCACCG CAACTGCAAG
    CTTCAGGTGT TGGAGTTAGA CAACTGCAGC CTCACATCAC ACTGCTGCTG GGATCTTTCC
    ACACTTCTAA CTTCTAACCA GAGCCTGCGA AAGCTGTGCC TAGGCAACAA CGACTTGGGT
    GATCTGGGGG TCATGCTTCT CTGTGAAGTG CTCAAACAGC AGGGCTGCCT CCTGAAAAGC
    CTGAGGTTGT GTGAAATGTA TTTCAACTAT GACACAAAAC GTGCCCTAGA AACACTTCAA
    GAAGAAAAGC CTGAATTAAC CATTGTCTTT GAGCCTTCTC GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327653.1
    CDS273..3197
    Translation

    Target ORF information:

    RefSeq Version XM_015472167.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472167.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGGG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGTTGGTG AATTCTGGCC TTACATCAGG TTGTTGTCCA
    GCTCTATCCT CAGTGCTCAG TACAAATCAG AATCTCACCC ACCTTTACCT ACAGGGCAAT
    GCTCTTGGAG ACATGGGGGT CAAGCTACTC TGTGAGGGAC TCTTGCACCG CAACTGCAAG
    CTTCAGGTGT TGGAGTTAGA CAACTGCAGC CTCACATCAC ACTGCTGCTG GGATCTTTCC
    ACACTTCTAA CTTCTAACCA GAGCCTGCGA AAGCTGTGCC TAGGCAACAA CGACTTGGGT
    GATCTGGGGG TCATGCTTCT CTGTGAAGTG CTCAAACAGC AGGGCTGCCT CCTGAAAAGC
    CTGAGGTTGT GTGAAATGTA TTTCAACTAT GACACAAAAC GTGCCCTAGA AACACTTCAA
    GAAGAAAAGC CTGAATTAAC CATTGTCTTT GAGCCTTCTC GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54010
    Clone ID Related Accession (Same CDS sequence) XM_015472168.1
    Accession Version XM_015472168.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104026.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGGG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGTTGGTC AGTTGCTGTC TCACATCGGC AAGCTGTGAG
    GACCTTGCAT CTGTCCTGAG CACCAACCAT TCCCTGACCA GACTCTACTT GGGGGAAAAT
    GCTCTGGGAG ACTCAGGAGT TGGGATTTTG TGTGAAAAAG TAAAGAATCC ACACTGTAAC
    TTGCAAAAAC TGGGGGCAAT GCTCTTGGAG ACATGGGGGT CAAGCTACTC TGTGAGGGAC
    TCTTGCACCG CAACTGCAAG CTTCAGGTGT TGGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327654.1
    CDS277..3015
    Translation

    Target ORF information:

    RefSeq Version XM_015472168.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 3 (NLRP3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472168.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGGG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGTTGGTC AGTTGCTGTC TCACATCGGC AAGCTGTGAG
    GACCTTGCAT CTGTCCTGAG CACCAACCAT TCCCTGACCA GACTCTACTT GGGGGAAAAT
    GCTCTGGGAG ACTCAGGAGT TGGGATTTTG TGTGAAAAAG TAAAGAATCC ACACTGTAAC
    TTGCAAAAAC TGGGGGCAAT GCTCTTGGAG ACATGGGGGT CAAGCTACTC TGTGAGGGAC
    TCTTGCACCG CAACTGCAAG CTTCAGGTGT TGGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236153
    Clone ID Related Accession (Same CDS sequence) XM_015472167.2
    Accession Version XM_015472167.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2925bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472167.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGCG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGTTGGTG AATTCTGGCC TTACATCAGG TTGTTGTCCA
    GCTCTATCCT CAGTGCTCAG TACAAATCAG AATCTCACCC ACCTTTACCT ACAGGGCAAT
    GCTCTTGGAG ACATGGGGGT CAAGCTACTC TGTGAGGGAC TCTTGCACCG CAACTGCAAG
    CTTCAGGTGT TGGAGTTAGA CAACTGCAGC CTCACATCAC ACTGCTGCTG GGATCTTTCC
    ACACTTCTAA CTTCTAACCA GAGCCTGCGA AAGCTGTGCC TAGGCAACAA CGACTTGGGT
    GATCTGGGGG TCATGCTTCT CTGTGAAGTG CTCAAACAGC AGGGCTGCCT CCTGAAAAGC
    CTGAGGTTGT GTGAAATGTA TTTCAACTAT GACACAAAAC GTGCCCTAGA AACACTTCAA
    GAAGAAAAGC CTGAATTAAC CATTGTCTTT GAGCCTTCTC GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327653.1
    CDS281..3205
    Translation

    Target ORF information:

    RefSeq Version XM_015472167.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472167.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGCG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGTTGGTG AATTCTGGCC TTACATCAGG TTGTTGTCCA
    GCTCTATCCT CAGTGCTCAG TACAAATCAG AATCTCACCC ACCTTTACCT ACAGGGCAAT
    GCTCTTGGAG ACATGGGGGT CAAGCTACTC TGTGAGGGAC TCTTGCACCG CAACTGCAAG
    CTTCAGGTGT TGGAGTTAGA CAACTGCAGC CTCACATCAC ACTGCTGCTG GGATCTTTCC
    ACACTTCTAA CTTCTAACCA GAGCCTGCGA AAGCTGTGCC TAGGCAACAA CGACTTGGGT
    GATCTGGGGG TCATGCTTCT CTGTGAAGTG CTCAAACAGC AGGGCTGCCT CCTGAAAAGC
    CTGAGGTTGT GTGAAATGTA TTTCAACTAT GACACAAAAC GTGCCCTAGA AACACTTCAA
    GAAGAAAAGC CTGAATTAAC CATTGTCTTT GAGCCTTCTC GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236154
    Clone ID Related Accession (Same CDS sequence) XM_015472168.2
    Accession Version XM_015472168.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472168.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGCG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGTTGGTC AGTTGCTGTC TCACATCGGC AAGCTGTGAG
    GACCTTGCAT CTGTCCTGAG CACCAACCAT TCCCTGACCA GACTCTACTT GGGGGAAAAT
    GCTCTGGGAG ACTCAGGAGT TGGGATTTTG TGTGAAAAAG TAAAGAATCC ACACTGTAAC
    TTGCAAAAAC TGGGGGCAAT GCTCTTGGAG ACATGGGGGT CAAGCTACTC TGTGAGGGAC
    TCTTGCACCG CAACTGCAAG CTTCAGGTGT TGGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327654.1
    CDS281..3019
    Translation

    Target ORF information:

    RefSeq Version XM_015472168.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 3 (NLRP3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472168.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGCG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGTTGGTC AGTTGCTGTC TCACATCGGC AAGCTGTGAG
    GACCTTGCAT CTGTCCTGAG CACCAACCAT TCCCTGACCA GACTCTACTT GGGGGAAAAT
    GCTCTGGGAG ACTCAGGAGT TGGGATTTTG TGTGAAAAAG TAAAGAATCC ACACTGTAAC
    TTGCAAAAAC TGGGGGCAAT GCTCTTGGAG ACATGGGGGT CAAGCTACTC TGTGAGGGAC
    TCTTGCACCG CAACTGCAAG CTTCAGGTGT TGGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236155
    Clone ID Related Accession (Same CDS sequence) XM_024994442.1
    Accession Version XM_024994442.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGCG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGGCAATG CTCTTGGAGA CATGGGGGTC AAGCTACTCT
    GTGAGGGACT CTTGCACCGC AACTGCAAGC TTCAGGTGTT GGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850210.1
    CDS281..2848
    Translation

    Target ORF information:

    RefSeq Version XM_024994442.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 3 (NLRP3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994442.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGCG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGGCAATG CTCTTGGAGA CATGGGGGTC AAGCTACTCT
    GTGAGGGACT CTTGCACCGC AACTGCAAGC TTCAGGTGTT GGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236152
    Clone ID Related Accession (Same CDS sequence) XM_010807177.3
    Accession Version XM_010807177.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807177.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGCG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGTTGGTC AGTTGCTGTC TCACATCGGC AAGCTGTGAG
    GACCTTGCAT CTGTCCTGAG CACCAACCAT TCCCTGACCA GACTCTACTT GGGGGAAAAT
    GCTCTGGGAG ACTCAGGAGT TGGGATTTTG TGTGAAAAAG TAAAGAATCC ACACTGTAAC
    TTGCAAAAAC TGGGGTTGGT GAATTCTGGC CTTACATCAG GTTGTTGTCC AGCTCTATCC
    TCAGTGCTCA GTACAAATCA GAATCTCACC CACCTTTACC TACAGGGCAA TGCTCTTGGA
    GACATGGGGG TCAAGCTACT CTGTGAGGGA CTCTTGCACC GCAACTGCAA GCTTCAGGTG
    TTGGAGTTAG ACAACTGCAG CCTCACATCA CACTGCTGCT GGGATCTTTC CACACTTCTA
    ACTTCTAACC AGAGCCTGCG AAAGCTGTGC CTAGGCAACA ACGACTTGGG TGATCTGGGG
    GTCATGCTTC TCTGTGAAGT GCTCAAACAG CAGGGCTGCC TCCTGAAAAG CCTGAGGTTG
    TGTGAAATGT ATTTCAACTA TGACACAAAA CGTGCCCTAG AAACACTTCA AGAAGAAAAG
    CCTGAATTAA CCATTGTCTT TGAGCCTTCT CGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805479.1
    CDS464..3559
    Translation

    Target ORF information:

    RefSeq Version XM_010807177.3
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807177.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGCG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGTTGGTC AGTTGCTGTC TCACATCGGC AAGCTGTGAG
    GACCTTGCAT CTGTCCTGAG CACCAACCAT TCCCTGACCA GACTCTACTT GGGGGAAAAT
    GCTCTGGGAG ACTCAGGAGT TGGGATTTTG TGTGAAAAAG TAAAGAATCC ACACTGTAAC
    TTGCAAAAAC TGGGGTTGGT GAATTCTGGC CTTACATCAG GTTGTTGTCC AGCTCTATCC
    TCAGTGCTCA GTACAAATCA GAATCTCACC CACCTTTACC TACAGGGCAA TGCTCTTGGA
    GACATGGGGG TCAAGCTACT CTGTGAGGGA CTCTTGCACC GCAACTGCAA GCTTCAGGTG
    TTGGAGTTAG ACAACTGCAG CCTCACATCA CACTGCTGCT GGGATCTTTC CACACTTCTA
    ACTTCTAACC AGAGCCTGCG AAAGCTGTGC CTAGGCAACA ACGACTTGGG TGATCTGGGG
    GTCATGCTTC TCTGTGAAGT GCTCAAACAG CAGGGCTGCC TCCTGAAAAG CCTGAGGTTG
    TGTGAAATGT ATTTCAACTA TGACACAAAA CGTGCCCTAG AAACACTTCA AGAAGAAAAG
    CCTGAATTAA CCATTGTCTT TGAGCCTTCT CGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21365
    Clone ID Related Accession (Same CDS sequence) XM_010807177.2 , NM_001102219.1
    Accession Version NM_001102219.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC147936.1. On Aug 21, 2007 this sequence version replaced XM_581687.3. ##Evidence-Data-START## Transcript exon combination :: BC147936.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145442 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGGG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGTTGGTC AGTTGCTGTC TCACATCGGC AAGCTGTGAG
    GACCTTGCAT CTGTCCTGAG CACCAACCAT TCCCTGACCA GACTCTACTT GGGGGAAAAT
    GCTCTGGGAG ACTCAGGAGT TGGGATTTTG TGTGAAAAAG TAAAGAATCC ACACTGTAAC
    TTGCAAAAAC TGGGGTTGGT GAATTCTGGC CTTACATCAG GTTGTTGTCC AGCTCTATCC
    TCAGTGCTCA GTACAAATCA GAATCTCACC CACCTTTACC TACAGGGCAA TGCTCTTGGA
    GACATGGGGG TCAAGCTACT CTGTGAGGGA CTCTTGCACC GCAACTGCAA GCTTCAGGTG
    TTGGAGTTAG ACAACTGCAG CCTCACATCA CACTGCTGCT GGGATCTTTC CACACTTCTA
    ACTTCTAACC AGAGCCTGCG AAAGCTGTGC CTAGGCAACA ACGACTTGGG TGATCTGGGG
    GTCATGCTTC TCTGTGAAGT GCTCAAACAG CAGGGCTGCC TCCTGAAAAG CCTGAGGTTG
    TGTGAAATGT ATTTCAACTA TGACACAAAA CGTGCCCTAG AAACACTTCA AGAAGAAAAG
    CCTGAATTAA CCATTGTCTT TGAGCCTTCT CGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001095689.1
    CDS96..3191
    Translation

    Target ORF information:

    RefSeq Version NM_001102219.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family pyrin domain containing 3 (NLRP3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102219.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGGG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGTTGGTC AGTTGCTGTC TCACATCGGC AAGCTGTGAG
    GACCTTGCAT CTGTCCTGAG CACCAACCAT TCCCTGACCA GACTCTACTT GGGGGAAAAT
    GCTCTGGGAG ACTCAGGAGT TGGGATTTTG TGTGAAAAAG TAAAGAATCC ACACTGTAAC
    TTGCAAAAAC TGGGGTTGGT GAATTCTGGC CTTACATCAG GTTGTTGTCC AGCTCTATCC
    TCAGTGCTCA GTACAAATCA GAATCTCACC CACCTTTACC TACAGGGCAA TGCTCTTGGA
    GACATGGGGG TCAAGCTACT CTGTGAGGGA CTCTTGCACC GCAACTGCAA GCTTCAGGTG
    TTGGAGTTAG ACAACTGCAG CCTCACATCA CACTGCTGCT GGGATCTTTC CACACTTCTA
    ACTTCTAACC AGAGCCTGCG AAAGCTGTGC CTAGGCAACA ACGACTTGGG TGATCTGGGG
    GTCATGCTTC TCTGTGAAGT GCTCAAACAG CAGGGCTGCC TCCTGAAAAG CCTGAGGTTG
    TGTGAAATGT ATTTCAACTA TGACACAAAA CGTGCCCTAG AAACACTTCA AGAAGAAAAG
    CCTGAATTAA CCATTGTCTT TGAGCCTTCT CGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21365
    Clone ID Related Accession (Same CDS sequence) XM_010807177.2 , NM_001102219.1
    Accession Version XM_010807177.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104026.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGGG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGTTGGTC AGTTGCTGTC TCACATCGGC AAGCTGTGAG
    GACCTTGCAT CTGTCCTGAG CACCAACCAT TCCCTGACCA GACTCTACTT GGGGGAAAAT
    GCTCTGGGAG ACTCAGGAGT TGGGATTTTG TGTGAAAAAG TAAAGAATCC ACACTGTAAC
    TTGCAAAAAC TGGGGTTGGT GAATTCTGGC CTTACATCAG GTTGTTGTCC AGCTCTATCC
    TCAGTGCTCA GTACAAATCA GAATCTCACC CACCTTTACC TACAGGGCAA TGCTCTTGGA
    GACATGGGGG TCAAGCTACT CTGTGAGGGA CTCTTGCACC GCAACTGCAA GCTTCAGGTG
    TTGGAGTTAG ACAACTGCAG CCTCACATCA CACTGCTGCT GGGATCTTTC CACACTTCTA
    ACTTCTAACC AGAGCCTGCG AAAGCTGTGC CTAGGCAACA ACGACTTGGG TGATCTGGGG
    GTCATGCTTC TCTGTGAAGT GCTCAAACAG CAGGGCTGCC TCCTGAAAAG CCTGAGGTTG
    TGTGAAATGT ATTTCAACTA TGACACAAAA CGTGCCCTAG AAACACTTCA AGAAGAAAAG
    CCTGAATTAA CCATTGTCTT TGAGCCTTCT CGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805479.1
    CDS455..3550
    Translation

    Target ORF information:

    RefSeq Version XM_010807177.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807177.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGAATGG TAAGTGTCCG CTGCAAGCTG GCTCGTTACC TGGAGGACCT GGAAGACATA 
    GACTTTAAGA AATTCAAGAT GCATTTAGAA GACTATCCCA GTCAGAAGGG CTGCACCTCA
    ATTCCTCGGG GTCAGACAGA AAAAGCAGAT CACGTGGATC TAGCCACTCT GATGATTGAT
    TTCAACGGGG AAGAGAAGGC ATGGGCCATG GCCAAGTGGA TTTTTGCTGC AATCAACAGG
    AGAGACCTTT ATGAGAAAGC TAAGAGGGAA GAGCCAGAAT GGGAGAATGC AAATATTTCT
    GTGCTAAGTC AGGAGGAAAG CCTTGAAGAA GAATGGATGG GTTTACTGGG ATACCTTTCC
    AGAATCTCTA TTTGTAGGAA AAAAAAAGAT TACTGTAAGA AGTACAGAAA ATATGTGAGA
    AGCAAATTCC AGTGTATTAA AGACAGGAAT GCCCGTCTGG GTGAGAGCGT GAACCTCAAC
    AAACGCTTCA CCAGGCTGCG TCTCATCAAG GAACACAGGA GCCAACAGGA GAGGGAGCAT
    GAGCTCCTGG CCATTGGTAG GACCTGGGCC AAGATACAGG ATAGCCCTGT GAGTTCTGTG
    AACTTGGAAT TGCTGTTTGA TCCTGAGGAC CAGCACTCTG AGCCTGTGCA CACAGTGGTA
    TTCCAGGGAG CAGCGGGCAT TGGGAAAACA ATACTGGCTA GGAAGATCAT GTTGGACTGG
    GCATCAGAGA AACTTTACCA GGATAGATTT GACTATTTGT TTTACATTCA CTGCCGGGAG
    GTGAGCCTTG GGACGCAGAG GAGTCTGGGG GACCTGATCG CCAGCTGCTG CCCTGGCCCA
    AACCCACCCA TAGGCAAGAT TGTAAGCAAG CCTTCCAGGA TCCTCTTCCT CATGGACGGC
    TTTGATGAGC TGCAAGGTGC CTTTGATGAG CACACAGAAG CACTCTGCAC AAACTGGCGG
    AAGGTGGAGC GGGGAGACAT TCTCCTGAGC AGCCTCATCA GAAAAAGACT GCTTCCTGAG
    GCCTCCCTCC TCATCACCAC AAGACCCGTG GCCCTGGAGA AACTTCAGCA CTTGCTGGGC
    CAGGCTCGTC ATGTGGAGAT CCTGGGTTTC TCAGAGGCCA GGAGGAAGGA ATATTTCTTA
    AAGTATTTCT CAGATGAGCA GCAAGCAAGG GAAGCCTTCA GACTGATTCA GGAGAATGAG
    ATCCTCTTCA CCATGTGCTT TATTCCTCTG GTCTGCTGGA TTGTGTGCAC TGGGCTGAAA
    CAGCAGATGG ATAGTGGTAA GAGTCTTGCT CGGACATCCA AGACCACCAC TGCAGTGTAT
    ATCTTCTTCC TCTCCAGTTT GTTGCAATCT CAGGGAGGGA GCCAGGAGAA CCACAACTCT
    GCTACCCTCT GGGGTCTCTG CTCACTGGCT GCAGATGGAA TCTGGAACCA GAAAATCCTA
    TTTCAGGAGT GTGATCTCAG AAATCATGGC CTGCAGAAGG CAGATGTGTC TGCTTTCTTG
    AGGATGAACC TGTTCCAAAA GGAAGTGGAC TGCGAGAAAT TCTACAGCTT CATCCACATG
    ACTTTCCAGG AGTTCTTTGC TGCCATGTAC TACCTGCTGG AAGAAGATAA TCATGGGGAG
    ATGAGGAACA CGCCTCAGGC TTGTTCAAAG CTTCCCAACC GAGATGTGAA GGTCCTTCTC
    GAAAACTACG GCAAATTCGA AAAGGGATAT CTGATTTTTG TTGTCCGTTT CCTCTTTGGC
    CTTATAAACC AGGAGAGAAC CTCCTACTTA GAGAAAAAAC TGAGTTGTAA GATCTCTCAG
    AAAATCAGGC TGGAGCTGCT GAAATGGATT GAAGCAAAAG CCAATGCCAA GACACTACAG
    ATTGAGCCCA GCCAGCTGGA ATTGTTCTAT TGTTTGTATG AGATGCAGGA GGAGGACTTT
    GTGCAAAGGG CCATGAGCCA TTTCCCCAAA ATTGAGATCA AGCTGTCCAC CAGAATGGAC
    CATGTGGTTT CTTCTTTTTG TATTGAGAAC TGTCGCCATG TGGAATCCCT TTCCTTGAGG
    TTGCTCCATA ACTCACCCAA GGAGGAGGAA GAAGAGGAGG AAGTTAGACA CTCTCATATG
    GACCGTTCTG TTCTCTCTGA TTTTGAGGTG GCATATTCTC AGGGATTGGT GAACTATCTG
    ACCTCCAGCA TTTGCAGGGG CATCTTCTCA GTCCTGAGCA ATAACTGGAA TCTCACTGAA
    TTGAACCTCA GTGGCAATAC CCTGGGAGAC CCAGGCATGA ACGTGTTATG TGAAACACTC
    CAACAACCTG GCTGTAACAT TCGTAGATTG TGGTTGGGAC AGTGCTGCCT TTCCCATCAG
    TGCTGCTTCA ACATCTCCTC AGTCTTGAGC AACAACCAGA AGCTGGTGGA GTTGGACCTG
    AGCCACAATG CCCTGGGAGA CTTCGGGATC AGACTTCTGT GTGTGGGACT GAGGCATCTA
    TTCTGCAATC TGAAGAAGCT CTGGTTGGTC AGTTGCTGTC TCACATCGGC AAGCTGTGAG
    GACCTTGCAT CTGTCCTGAG CACCAACCAT TCCCTGACCA GACTCTACTT GGGGGAAAAT
    GCTCTGGGAG ACTCAGGAGT TGGGATTTTG TGTGAAAAAG TAAAGAATCC ACACTGTAAC
    TTGCAAAAAC TGGGGTTGGT GAATTCTGGC CTTACATCAG GTTGTTGTCC AGCTCTATCC
    TCAGTGCTCA GTACAAATCA GAATCTCACC CACCTTTACC TACAGGGCAA TGCTCTTGGA
    GACATGGGGG TCAAGCTACT CTGTGAGGGA CTCTTGCACC GCAACTGCAA GCTTCAGGTG
    TTGGAGTTAG ACAACTGCAG CCTCACATCA CACTGCTGCT GGGATCTTTC CACACTTCTA
    ACTTCTAACC AGAGCCTGCG AAAGCTGTGC CTAGGCAACA ACGACTTGGG TGATCTGGGG
    GTCATGCTTC TCTGTGAAGT GCTCAAACAG CAGGGCTGCC TCCTGAAAAG CCTGAGGTTG
    TGTGAAATGT ATTTCAACTA TGACACAAAA CGTGCCCTAG AAACACTTCA AGAAGAAAAG
    CCTGAATTAA CCATTGTCTT TGAGCCTTCT CGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.