COL5A2 cDNA ORF clone, Bos taurus(cattle)

The following COL5A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227645 XM_024979774.1
Latest version!
Bos taurus collagen type V alpha 2 chain (COL5A2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa53936 XM_010801836.2
Latest version!
Bos taurus collagen type V alpha 2 (COL5A2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa89500 XM_015462240.1
Latest version!
Bos taurus collagen type V alpha 2 (COL5A2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa227645
    Clone ID Related Accession (Same CDS sequence) XM_024979774.1
    Accession Version XM_024979774.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-2(V) chain
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGATGGCAA ACTGGGTGGA AGCAAGACCT CTGCTCATTC TTACTGTTTT ATTAGGGCAG 
    TTCGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC
    TGCACCCAGA ATGGTCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGCCAG
    ATCTGTGTCT GTGACAATGG CGCCATTCTT TGTGACAAGA TTCAGTGCCA GGATGTGCTT
    GAGTGTGCTG ACCCCGTAAC TCCCCCTGGG GAATGCTGTC CTGTCTGTCC ACATACAACT
    GGAGGTGGCA ATACCAACTT TGGAAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA
    GTACCTGTTG TGACAGGCAT ACGTGGACGT CCAGGACCTG CAGGACCACC AGGATCACAA
    GGACCGAGAG GAGAGAGAGG CCCCAAAGGA AAACCTGGGC CTCGTGGTCC TCAGGGAATC
    GATGGAGAGC CAGGTGTTCC TGGTCAGCCA GGTGCCCCAG GACCTCCTGG GCATCCCTCT
    CACCCAGGAC CGGATGGCAT GAGCAGGCCG TTTTCAGCTC AGATGGCTGG GTTAGATGAG
    AAGTCTGGAC TTGGAAGTCA AGTAGGACTG ATGCCTGGAT CCGTGGGTCC TGTTGGCCCA
    AGGGGTCCTC AAGGTTTACA AGGGCAGCAG GGTGGTGTTG GCCCAGCAGG ACCTCCTGGT
    GAACCTGGTG ACCCTGGCCC AATGGGTCCA GTTGGTGCAC GAGGACCAGA GGGCCCTCCT
    GGAAAACCTG GTGAAGATGG TGAGCCTGGT AGAAATGGAA AACCTGGTGA AGTGGGATTT
    GCAGGATCTC CGGGAGCTCG AGGATTTCCT GGGGCTCCTG GTCTTCCAGG CCTGAAGGGT
    CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCAAC TGGTTCCAAG
    GGTGAAGCTG GCCCTACTGG TCCAATGGGT GCCATGGGTC CGATGGGCCC AAGAGGAATG
    CCAGGAGAGA GAGGAAGGCT TGGACCACAG GGTGCTCCTG GACAACGAGG TGCGCATGGT
    ATGCCTGGAA AACCTGGGCC CATGGGTCCT CTTGGGATAC CTGGCTCTGC TGGTTTTCCA
    GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCCACTGGGG CGCGAGGCCC TGAAGGTCCT
    CAAGGGCAAA GAGGGGAAAC CGGGCCCCCT GGTCCTGTTG GCTCCCAAGG TCTTCCTGGT
    GCAGTAGGAA CTGATGGTAC GCCTGGTGCC AAAGGCCCAA CGGGCTCTCC GGGTACTTCT
    GGTCCTCCTG GCTTAGCAGG ACCCCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGACCT
    CCCGGAATTC GAGGCCAACC GGGTGATCCA GGAGTCCCAG GTTTCAAAGG AGAAGCTGGA
    CCAAAAGGGG AGCCGGGGCC ACATGGTGTC CAGGGTCCAA TCGGCCCACC TGGTGAAGAA
    GGCAAACGAG GCCCCCGAGG TGATCCGGGC ACAGTTGGTC CTCCGGGCCC AGTGGGAGAA
    AGGGGTGCTC CTGGCAATCG TGGCTTTCCA GGCTCTGATG GTTTACCTGG ACCAAAGGGC
    GCTCAAGGAG AGAGGGGTCC TGTTGGCTCT TCAGGACCTA AAGGAGGTCA GGGGGATCCA
    GGACGTCCAG GTGAACCTGG GCTTCCAGGT GCTCGGGGTC TGACAGGAAA TCCTGGTGTC
    CAGGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCTCCAG GAGAAGACGG CCGGCCGGGT
    CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCTGGGAGTA TGGGTCTTCC AGGCCCCAAA
    GGTAGCAGTG GTGATCCTGG AAAACCTGGA GAAGCAGGAA ATGCTGGTGT TCCTGGGCAG
    AGGGGAGCTC CTGGAAAAGA TGGCGAAGTT GGTCCTTCTG GTCCTGTGGG GCCCCCGGGT
    CTAGCCGGGG AGAGAGGAGA ACAGGGTCCT CCAGGCCCCA CTGGCTTTCA GGGGCTTCCT
    GGTCCTCCAG GGCCTCCTGG GGAAGCTGGA AAGCCAGGTG ATCAGGGGGT TCCTGGAGAT
    CCTGGGGCAG TTGGCCCACT AGGACCTAGA GGAGAACGAG GAAATCCAGG GGAAAGAGGA
    GAACCAGGAA TAACTGGACT TCCTGGTGAG AAAGGAATGG CTGGAGGACA TGGTCCTGAT
    GGTCCAAAAG GCAGTCCAGG ACCACCTGGA ACCCCTGGAG ATACAGGCCC ACCAGGGCTT
    CAAGGTATGC CAGGAGAAAG GGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT
    GGCATAGGAG AAAAAGGTGC TGAAGGTACA GCTGGAAATG ATGGAGCAAG AGGTCTTCCG
    GGTCCCTTGG GCCCTCCAGG TCCTTCAGGC CCTACTGGAG AAAAGGGAGA ACCCGGCCCT
    CGAGGTTTAG TCGGCCCGCC TGGCTCCCGT GGCAATCCCG GCTCACGTGG TGAAAATGGC
    CCAACTGGAG CTGTGGGTTT TGCGGGACCC CAGGGGCCGG ATGGGCAGCC TGGAGTGAAA
    GGTGAACCTG GAGAGCCTGG GCAGAAGGGA GACGCTGGCT CTCCTGGACC ACAAGGACTG
    GCAGGATCCC CAGGGCCTCA TGGTCCTAAT GGTGTTCCCG GACTGAAAGG TGGTCGAGGA
    ACTCAGGGTC CACCTGGTGC TACAGGATTT CCTGGTTCTG CTGGCAGAGT TGGACCTCCA
    GGTCCTGCTG GAGCTCCAGG ACCTGCGGGG CCCTTAGGGG AGCCCGGGAA GGAAGGACCT
    CCAGGTCTTC GTGGGGACCC CGGGTCTCAT GGACGAGTAG GAGATCGAGG ACCAGCTGGC
    CCTCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGACAACC TGGTCCAGAT
    GGTCCCCCTG GTCCAGCTGG AACTACTGGG CAAAGAGGAA TCGTCGGCAT GCCGGGGCAG
    CGTGGGGAGA GAGGGATGCC CGGCCTTCCA GGCCCAGCAG GCACACCAGG AAAAGTAGGA
    CCAACTGGTG CCCCAGGAGA TAAAGGTCCA CCTGGACCTG TTGGGCCCCC GGGCTCGAAT
    GGCCCTGTGG GGGAACCAGG ACCGGAGGGT CCAGCTGGTA ATGATGGTAC TCCAGGACGG
    GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC
    TCTCAGGGTG CCCCTGGAAC TCCTGGTCCT GTTGGTGCTC CGGGAGATGC AGGACAAAGA
    GGAGACCCGG GTTCTCGAGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGTTTA
    CTTGGACCGC AAGGACCTCG TGGTGACAAA GGCGATCATG GAGACAGAGG TGATCGAGGT
    CAGAAGGGTC ACAGAGGCTT TACTGGTCTT CAGGGTCTTC CTGGACCTCC TGGTCCAACT
    GGTGAACAAG GCAGTGCTGG AATCCCTGGA CCATTTGGCC CAAGAGGACC CCCGGGTCCA
    GTTGGTCCTT CAGGCAAAGA AGGAAGCCCT GGGCCACTTG GGCCCATTGG GCCTCCTGGT
    GTGCGGGGCA GCGTTGGAGA AGCAGGCCCT GAGGGTCCTC CTGGTGAGCC TGGTCCCCCT
    GGCCCTCCGG GACCCCCTGG CCACCTTACA GCTGCTCTTG GGGACATCAT GGGGCACTAT
    GATGACAGCA TGCCAGACCC ACTTCCGGAG TTTACTGAAG ATCAGGCGGC TCCTGATGAC
    AAAAACAAAA CGGACCCGGG GGTACATGCG ACCCTGAAGT CACTCAGTAG TCAGATTGAA
    ACCATGCGCA GCCCTGATGG CTCTAGAAAG CACCCTGCCC GGACTTGTGA TGATTTAAAG
    CTTTGCCATT CTGCAAAGAA GAGCGGAGAG TACTGGATTG ACCCTAACCA GGGATCTGCT
    GAAGATGCAA TCAAAGTTTA CTGCAACATG GAAACAGGAG AAACATGTAT TTCAGCAAAT
    CCATCCAGTG TACCACGTAA AACCTGGTGG GCCAGCAAAT CTCCTGATAA TAAGCCTGTT
    TGGTATGGTC TTGATATGAA TCGAGGATCT CAGTTTGTTT ATGGAGACCA CCAGTCACCT
    AATGCAGCCA TTACTCAGAT GACCTTCTTG CGCCTTTTAT CGAAAGAAGC CTCCCAGAAC
    ATCACCTACA TCTGTAAAAA CAGTGTAGGA TACATGGATG ATCAAGCTAA GAACTTGAAG
    AAAGCTGTGG TTCTCAAAGG GTCAAATGAC TTAGAAATCA AAGCAGAGGG AAACGTTAGA
    TTCAGATACA TAGTTCTTCA TGATTCTTGC TCTAAACGAA ATGGAAACGT GGGCAAGACC
    ATCTTTGAAT ATAGAACGCA GAATGTGGCA CGCTTGCCCA TCATAGATCT TGCCCCTGTG
    GATGTTGGCA GTACAGACCA AGAATTTGGC GTTGAAATTG GGCCAGTTTG TTTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835542.1
    CDS417..4916
    Translation

    Target ORF information:

    RefSeq Version XM_024979774.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 2 chain (COL5A2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979774.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGATGGCAA ACTGGGTGGA AGCAAGACCT CTGCTCATTC TTACTGTTTT ATTAGGGCAG 
    TTCGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC
    TGCACCCAGA ATGGTCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGCCAG
    ATCTGTGTCT GTGACAATGG CGCCATTCTT TGTGACAAGA TTCAGTGCCA GGATGTGCTT
    GAGTGTGCTG ACCCCGTAAC TCCCCCTGGG GAATGCTGTC CTGTCTGTCC ACATACAACT
    GGAGGTGGCA ATACCAACTT TGGAAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA
    GTACCTGTTG TGACAGGCAT ACGTGGACGT CCAGGACCTG CAGGACCACC AGGATCACAA
    GGACCGAGAG GAGAGAGAGG CCCCAAAGGA AAACCTGGGC CTCGTGGTCC TCAGGGAATC
    GATGGAGAGC CAGGTGTTCC TGGTCAGCCA GGTGCCCCAG GACCTCCTGG GCATCCCTCT
    CACCCAGGAC CGGATGGCAT GAGCAGGCCG TTTTCAGCTC AGATGGCTGG GTTAGATGAG
    AAGTCTGGAC TTGGAAGTCA AGTAGGACTG ATGCCTGGAT CCGTGGGTCC TGTTGGCCCA
    AGGGGTCCTC AAGGTTTACA AGGGCAGCAG GGTGGTGTTG GCCCAGCAGG ACCTCCTGGT
    GAACCTGGTG ACCCTGGCCC AATGGGTCCA GTTGGTGCAC GAGGACCAGA GGGCCCTCCT
    GGAAAACCTG GTGAAGATGG TGAGCCTGGT AGAAATGGAA AACCTGGTGA AGTGGGATTT
    GCAGGATCTC CGGGAGCTCG AGGATTTCCT GGGGCTCCTG GTCTTCCAGG CCTGAAGGGT
    CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCAAC TGGTTCCAAG
    GGTGAAGCTG GCCCTACTGG TCCAATGGGT GCCATGGGTC CGATGGGCCC AAGAGGAATG
    CCAGGAGAGA GAGGAAGGCT TGGACCACAG GGTGCTCCTG GACAACGAGG TGCGCATGGT
    ATGCCTGGAA AACCTGGGCC CATGGGTCCT CTTGGGATAC CTGGCTCTGC TGGTTTTCCA
    GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCCACTGGGG CGCGAGGCCC TGAAGGTCCT
    CAAGGGCAAA GAGGGGAAAC CGGGCCCCCT GGTCCTGTTG GCTCCCAAGG TCTTCCTGGT
    GCAGTAGGAA CTGATGGTAC GCCTGGTGCC AAAGGCCCAA CGGGCTCTCC GGGTACTTCT
    GGTCCTCCTG GCTTAGCAGG ACCCCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGACCT
    CCCGGAATTC GAGGCCAACC GGGTGATCCA GGAGTCCCAG GTTTCAAAGG AGAAGCTGGA
    CCAAAAGGGG AGCCGGGGCC ACATGGTGTC CAGGGTCCAA TCGGCCCACC TGGTGAAGAA
    GGCAAACGAG GCCCCCGAGG TGATCCGGGC ACAGTTGGTC CTCCGGGCCC AGTGGGAGAA
    AGGGGTGCTC CTGGCAATCG TGGCTTTCCA GGCTCTGATG GTTTACCTGG ACCAAAGGGC
    GCTCAAGGAG AGAGGGGTCC TGTTGGCTCT TCAGGACCTA AAGGAGGTCA GGGGGATCCA
    GGACGTCCAG GTGAACCTGG GCTTCCAGGT GCTCGGGGTC TGACAGGAAA TCCTGGTGTC
    CAGGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCTCCAG GAGAAGACGG CCGGCCGGGT
    CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCTGGGAGTA TGGGTCTTCC AGGCCCCAAA
    GGTAGCAGTG GTGATCCTGG AAAACCTGGA GAAGCAGGAA ATGCTGGTGT TCCTGGGCAG
    AGGGGAGCTC CTGGAAAAGA TGGCGAAGTT GGTCCTTCTG GTCCTGTGGG GCCCCCGGGT
    CTAGCCGGGG AGAGAGGAGA ACAGGGTCCT CCAGGCCCCA CTGGCTTTCA GGGGCTTCCT
    GGTCCTCCAG GGCCTCCTGG GGAAGCTGGA AAGCCAGGTG ATCAGGGGGT TCCTGGAGAT
    CCTGGGGCAG TTGGCCCACT AGGACCTAGA GGAGAACGAG GAAATCCAGG GGAAAGAGGA
    GAACCAGGAA TAACTGGACT TCCTGGTGAG AAAGGAATGG CTGGAGGACA TGGTCCTGAT
    GGTCCAAAAG GCAGTCCAGG ACCACCTGGA ACCCCTGGAG ATACAGGCCC ACCAGGGCTT
    CAAGGTATGC CAGGAGAAAG GGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT
    GGCATAGGAG AAAAAGGTGC TGAAGGTACA GCTGGAAATG ATGGAGCAAG AGGTCTTCCG
    GGTCCCTTGG GCCCTCCAGG TCCTTCAGGC CCTACTGGAG AAAAGGGAGA ACCCGGCCCT
    CGAGGTTTAG TCGGCCCGCC TGGCTCCCGT GGCAATCCCG GCTCACGTGG TGAAAATGGC
    CCAACTGGAG CTGTGGGTTT TGCGGGACCC CAGGGGCCGG ATGGGCAGCC TGGAGTGAAA
    GGTGAACCTG GAGAGCCTGG GCAGAAGGGA GACGCTGGCT CTCCTGGACC ACAAGGACTG
    GCAGGATCCC CAGGGCCTCA TGGTCCTAAT GGTGTTCCCG GACTGAAAGG TGGTCGAGGA
    ACTCAGGGTC CACCTGGTGC TACAGGATTT CCTGGTTCTG CTGGCAGAGT TGGACCTCCA
    GGTCCTGCTG GAGCTCCAGG ACCTGCGGGG CCCTTAGGGG AGCCCGGGAA GGAAGGACCT
    CCAGGTCTTC GTGGGGACCC CGGGTCTCAT GGACGAGTAG GAGATCGAGG ACCAGCTGGC
    CCTCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGACAACC TGGTCCAGAT
    GGTCCCCCTG GTCCAGCTGG AACTACTGGG CAAAGAGGAA TCGTCGGCAT GCCGGGGCAG
    CGTGGGGAGA GAGGGATGCC CGGCCTTCCA GGCCCAGCAG GCACACCAGG AAAAGTAGGA
    CCAACTGGTG CCCCAGGAGA TAAAGGTCCA CCTGGACCTG TTGGGCCCCC GGGCTCGAAT
    GGCCCTGTGG GGGAACCAGG ACCGGAGGGT CCAGCTGGTA ATGATGGTAC TCCAGGACGG
    GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC
    TCTCAGGGTG CCCCTGGAAC TCCTGGTCCT GTTGGTGCTC CGGGAGATGC AGGACAAAGA
    GGAGACCCGG GTTCTCGAGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGTTTA
    CTTGGACCGC AAGGACCTCG TGGTGACAAA GGCGATCATG GAGACAGAGG TGATCGAGGT
    CAGAAGGGTC ACAGAGGCTT TACTGGTCTT CAGGGTCTTC CTGGACCTCC TGGTCCAACT
    GGTGAACAAG GCAGTGCTGG AATCCCTGGA CCATTTGGCC CAAGAGGACC CCCGGGTCCA
    GTTGGTCCTT CAGGCAAAGA AGGAAGCCCT GGGCCACTTG GGCCCATTGG GCCTCCTGGT
    GTGCGGGGCA GCGTTGGAGA AGCAGGCCCT GAGGGTCCTC CTGGTGAGCC TGGTCCCCCT
    GGCCCTCCGG GACCCCCTGG CCACCTTACA GCTGCTCTTG GGGACATCAT GGGGCACTAT
    GATGACAGCA TGCCAGACCC ACTTCCGGAG TTTACTGAAG ATCAGGCGGC TCCTGATGAC
    AAAAACAAAA CGGACCCGGG GGTACATGCG ACCCTGAAGT CACTCAGTAG TCAGATTGAA
    ACCATGCGCA GCCCTGATGG CTCTAGAAAG CACCCTGCCC GGACTTGTGA TGATTTAAAG
    CTTTGCCATT CTGCAAAGAA GAGCGGAGAG TACTGGATTG ACCCTAACCA GGGATCTGCT
    GAAGATGCAA TCAAAGTTTA CTGCAACATG GAAACAGGAG AAACATGTAT TTCAGCAAAT
    CCATCCAGTG TACCACGTAA AACCTGGTGG GCCAGCAAAT CTCCTGATAA TAAGCCTGTT
    TGGTATGGTC TTGATATGAA TCGAGGATCT CAGTTTGTTT ATGGAGACCA CCAGTCACCT
    AATGCAGCCA TTACTCAGAT GACCTTCTTG CGCCTTTTAT CGAAAGAAGC CTCCCAGAAC
    ATCACCTACA TCTGTAAAAA CAGTGTAGGA TACATGGATG ATCAAGCTAA GAACTTGAAG
    AAAGCTGTGG TTCTCAAAGG GTCAAATGAC TTAGAAATCA AAGCAGAGGG AAACGTTAGA
    TTCAGATACA TAGTTCTTCA TGATTCTTGC TCTAAACGAA ATGGAAACGT GGGCAAGACC
    ATCTTTGAAT ATAGAACGCA GAATGTGGCA CGCTTGCCCA TCATAGATCT TGCCCCTGTG
    GATGTTGGCA GTACAGACCA AGAATTTGGC GTTGAAATTG GGCCAGTTTG TTTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53936
    Clone ID Related Accession (Same CDS sequence) XM_010801836.2
    Accession Version XM_010801836.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-2(V) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103830.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801836.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGATGGCAA ACTGGGTGGA AGCAAGACCT CTGCTCATTC TTACTGTTTT ATTAGGGCAG 
    TTCGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC
    TGCACCCAGA ATGGTCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGCCAG
    ATCTGTGTCT GTGACAATGG CGCCATTCTT TGTGACAAGA TTCAGTGCCA GGATGTGCTT
    GAGTGTGCTG ACCCCGTAAC TCCCCCTGGG GAATGCTGTC CTGTCTGTCC ACATACAACT
    GGAGGTGGCA ATACCAACTT TGGAAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA
    GTACCTGTTG TGACAGGCAT ACGTGGACGT CCAGGACCTG CAGGACCACC AGGATCACAA
    GGACCGAGAG GAGAGAGAGG CCCCAAAGGA AAACCTGGGC CTCGTGGTCC TCAGGGAATC
    GATGGAGAGC CAGGTGTTCC TGGTCAGCCA GGTGCCCCAG GACCTCCTGG GCATCCCTCT
    CACCCAGGAC CGGATGGCAT GAGCAGGCCG TTTTCAGCTC AGATGGCTGG GTTAGATGAG
    AAGTCTGGAC TTGGAAGTCA AGTAGGACTG ATGCCTGGAT CCGTGGGTCC TGTTGGCCCA
    AGGGGTCCTC AAGGTTTACA AGGGCAGCAG GGTGGTGTTG GCCCAGCAGG ACCTCCTGGT
    GAACCTGGTG ACCCTGGCCC AATGGGTCCA GTTGGTGCAC GAGGACCAGA GGGCCCTCCT
    GGAAAACCTG GTGAAGATGG TGAGCCTGGT AGAAATGGAA AACCTGGTGA AGTGGGATTT
    GCAGGATCTC CGGGAGCTCG AGGATTTCCT GGGGCTCCTG GTCTTCCAGG CCTGAAGGGT
    CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCAAC TGGTTCCAAG
    GGTGAAGCTG GCCCTACTGG TCCAATGGGT GCCATGGGTC CGATGGGCCC AAGAGGAATG
    CCAGGAGAGA GAGGAAGGCT TGGACCACAG GGTGCTCCTG GACAACGAGG TGCGCATGGT
    ATGCCTGGAA AACCTGGGCC CATGGGTCCT CTTGGGATAC CTGGCTCTGC TGGTTTTCCA
    GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCCACTGGGG CGCGAGGCCC TGAAGGTCCT
    CAAGGGCAAA GAGGGGAAAC CGGGCCCCCT GGTCCTGTTG GCTCCCAAGG TCTTCCTGGT
    GCAGTAGGAA CTGATGGTAC GCCTGGTGCC AAAGGCCCAA CGGGCTCTCC GGGTACTTCT
    GGTCCTCCTG GCTTAGCAGG ACCCCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGACCT
    CCCGGAATTC GAGGCCAACC GGGTGATCCA GGAGTCCCAG GTTTCAAAGG AGAAGCTGGA
    CCAAAAGGGG AGCCGGGGCC ACATGGTGTC CAGGGTCCAA TCGGCCCACC TGGTGAAGAA
    GGCAAACGAG GCCCCCGAGG TGATCCGGGC ACAGTTGGTC CTCCGGGCCC AGTGGGAGAA
    AGGGGTATTC CTTTTTTTCC TGACGTGTTC TACTTTGCTT TGAAACAGGG CGCTCAAGGA
    GAGAGGGGTC CTGTTGGCTC TTCAGGACCT AAAGGAGGTC AGGGGGATCC AGGACGTCCA
    GGTGAACCTG GGCTTCCAGG TGCTCGGGGT CTGACAGGAA ATCCTGGTGT CCAGGGTCCT
    GAAGGAAAAC TTGGACCTTT GGGTGCTCCA GGAGAAGACG GCCGGCCGGG TCCTCCAGGC
    TCCATAGGAA TCAGAGGGCA GCCTGGGAGT ATGGGTCTTC CAGGCCCCAA AGGTAGCAGT
    GGTGATCCTG GAAAACCTGG AGAAGCAGGA AATGCTGGTG TTCCTGGGCA GAGGGGAGCT
    CCTGGAAAAG ATGGCGAAGT TGGTCCTTCT GGTCCTGTGG GGCCCCCGGG TCTAGCCGGG
    GAGAGAGGAG AACAGGGTCC TCCAGGCCCC ACTGGCTTTC AGGGGCTTCC TGGTCCTCCA
    GGGCCTCCTG GGGAAGCTGG AAAGCCAGGT GATCAGGGGG TTCCTGGAGA TCCTGGGGCA
    GTTGGCCCAC TAGGACCTAG AGGAGAACGA GGAAATCCAG GGGAAAGAGG AGAACCAGGA
    ATAACTGGAC TTCCTGGTGA GAAAGGAATG GCTGGAGGAC ATGGTCCTGA TGGTCCAAAA
    GGCAGTCCAG GACCACCTGG AACCCCTGGA GATACAGGCC CACCAGGGCT TCAAGGTATG
    CCAGGAGAAA GGGGAATTGC AGGAACTCCT GGCCCCAAGG GTGACAGAGG TGGCATAGGA
    GAAAAAGGTG CTGAAGGTAC AGCTGGAAAT GATGGAGCAA GAGGTCTTCC GGGTCCCTTG
    GGCCCTCCAG GTCCTTCAGG CCCTACTGGA GAAAAGGGAG AACCCGGCCC TCGAGGTTTA
    GTCGGCCCGC CTGGCTCCCG TGGCAATCCC GGCTCACGTG GTGAAAATGG CCCAACTGGA
    GCTGTGGGTT TTGCGGGACC CCAGGGGCCG GATGGGCAGC CTGGAGTGAA AGGTGAACCT
    GGAGAGCCTG GGCAGAAGGG AGACGCTGGC TCTCCTGGAC CACAAGGACT GGCAGGATCC
    CCAGGGCCTC ATGGTCCTAA TGGTGTTCCC GGACTGAAAG GTGGTCGAGG AACTCAGGGT
    CCACCTGGTG CTACAGGATT TCCTGGTTCT GCTGGCAGAG TTGGACCTCC AGGTCCTGCT
    GGAGCTCCAG GACCTGCGGG GCCCTTAGGG GAGCCCGGGA AGGAAGGACC TCCAGGTCTT
    CGTGGGGACC CCGGGTCTCA TGGACGAGTA GGAGATCGAG GACCAGCTGG CCCTCCTGGT
    GGCCCAGGAG ACAAAGGGGA CCCAGGAGAA GATGGACAAC CTGGTCCAGA TGGTCCCCCT
    GGTCCAGCTG GAACTACTGG GCAAAGAGGA ATCGTCGGCA TGCCGGGGCA GCGTGGGGAG
    AGAGGGATGC CCGGCCTTCC AGGCCCAGCA GGCACACCAG GAAAAGTAGG ACCAACTGGT
    GCCCCAGGAG ATAAAGGTCC ACCTGGACCT GTTGGGCCCC CGGGCTCGAA TGGCCCTGTG
    GGGGAACCAG GACCGGAGGG TCCAGCTGGT AATGATGGTA CTCCAGGACG GGATGGTGCT
    GTTGGAGAAC GTGGTGATCG TGGAGACCCT GGGCCTGCAG GTCTGCCAGG CTCTCAGGGT
    GCCCCTGGAA CTCCTGGTCC TGTTGGTGCT CCGGGAGATG CAGGACAAAG AGGAGACCCG
    GGTTCTCGAG GTCCTATAGG ACCACCTGGT CGAGCTGGGA AACGTGGTTT ACTTGTAACA
    AGGGGTCCAA CTGGTGAACA AGGCAGTGCT GGAATCCCTG GACCATTTGG CCCAAGAGGA
    CCCCCGGGTC CAGTTGGTCC TTCAGGCAAA GAAGGAAGCC CTGGGCCACT TGGGCCCATT
    GGGCCTCCTG GTGTGCGGGG CAGCGTTGGA GAAGCAGGCC CTGAGGGTCC TCCTGGTGAG
    CCTGGTCCCC CTGGCCCTCC GGGACCCCCT GGCCACCTTA CAGCTGCTCT TGGGGACATC
    ATGGGGCACT ATGATGACAG CATGCCAGAC CCACTTCCGG AGTTTACTGA AGATCAGGCG
    GCTCCTGATG ACAAAAACAA AACGGACCCG GGGGTACATG CGACCCTGAA GTCACTCAGT
    AGTCAGATTG AAACCATGCG CAGCCCTGAT GGCTCTAGAA AGCACCCTGC CCGGACTTGT
    GATGATTTAA AGCTTTGCCA TTCTGCAAAG AAGAGCGGAG AGTACTGGAT TGACCCTAAC
    CAGGGATCTG CTGAAGATGC AATCAAAGTT TACTGCAACA TGGAAACAGG AGAAACATGT
    ATTTCAGCAA ATCCATCCAG TGTACCACGT AAAACCTGGT GGGCCAGCAA ATCTCCTGAT
    AATAAGCCTG TTTGGTATGG TCTTGATATG AATCGAGGAT CTCAGTTTGT TTATGGAGAC
    CACCAGTCAC CTAATGCAGC CATTACTCAG ATGACCTTCT TGCGCCTTTT ATCGAAAGAA
    GCCTCCCAGA ACATCACCTA CATCTGTAAA AACAGTGTAG GATACATGGA TGATCAAGCT
    AAGAACTTGA AGAAAGCTGT GGTTCTCAAA GGGTCAAATG ACTTAGAAAT CAAAGCAGAG
    GGAAACGTTA GATTCAGATA CATAGTTCTT CATGATTCTT GCTCTAAACG AAATGGAAAC
    GTGGGCAAGA CCATCTTTGA ATATAGAACG CAGAATGTGG CACGCTTGCC CATCATAGAT
    CTTGCCCCTG TGGATGTTGG CAGTACAGAC CAAGAATTTG GCGTTGAAAT TGGGCCAGTT
    TGTTTTGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800138.1
    CDS409..4800
    Translation

    Target ORF information:

    RefSeq Version XM_010801836.2
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 2 (COL5A2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801836.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGATGGCAA ACTGGGTGGA AGCAAGACCT CTGCTCATTC TTACTGTTTT ATTAGGGCAG 
    TTCGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC
    TGCACCCAGA ATGGTCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGCCAG
    ATCTGTGTCT GTGACAATGG CGCCATTCTT TGTGACAAGA TTCAGTGCCA GGATGTGCTT
    GAGTGTGCTG ACCCCGTAAC TCCCCCTGGG GAATGCTGTC CTGTCTGTCC ACATACAACT
    GGAGGTGGCA ATACCAACTT TGGAAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA
    GTACCTGTTG TGACAGGCAT ACGTGGACGT CCAGGACCTG CAGGACCACC AGGATCACAA
    GGACCGAGAG GAGAGAGAGG CCCCAAAGGA AAACCTGGGC CTCGTGGTCC TCAGGGAATC
    GATGGAGAGC CAGGTGTTCC TGGTCAGCCA GGTGCCCCAG GACCTCCTGG GCATCCCTCT
    CACCCAGGAC CGGATGGCAT GAGCAGGCCG TTTTCAGCTC AGATGGCTGG GTTAGATGAG
    AAGTCTGGAC TTGGAAGTCA AGTAGGACTG ATGCCTGGAT CCGTGGGTCC TGTTGGCCCA
    AGGGGTCCTC AAGGTTTACA AGGGCAGCAG GGTGGTGTTG GCCCAGCAGG ACCTCCTGGT
    GAACCTGGTG ACCCTGGCCC AATGGGTCCA GTTGGTGCAC GAGGACCAGA GGGCCCTCCT
    GGAAAACCTG GTGAAGATGG TGAGCCTGGT AGAAATGGAA AACCTGGTGA AGTGGGATTT
    GCAGGATCTC CGGGAGCTCG AGGATTTCCT GGGGCTCCTG GTCTTCCAGG CCTGAAGGGT
    CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCAAC TGGTTCCAAG
    GGTGAAGCTG GCCCTACTGG TCCAATGGGT GCCATGGGTC CGATGGGCCC AAGAGGAATG
    CCAGGAGAGA GAGGAAGGCT TGGACCACAG GGTGCTCCTG GACAACGAGG TGCGCATGGT
    ATGCCTGGAA AACCTGGGCC CATGGGTCCT CTTGGGATAC CTGGCTCTGC TGGTTTTCCA
    GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCCACTGGGG CGCGAGGCCC TGAAGGTCCT
    CAAGGGCAAA GAGGGGAAAC CGGGCCCCCT GGTCCTGTTG GCTCCCAAGG TCTTCCTGGT
    GCAGTAGGAA CTGATGGTAC GCCTGGTGCC AAAGGCCCAA CGGGCTCTCC GGGTACTTCT
    GGTCCTCCTG GCTTAGCAGG ACCCCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGACCT
    CCCGGAATTC GAGGCCAACC GGGTGATCCA GGAGTCCCAG GTTTCAAAGG AGAAGCTGGA
    CCAAAAGGGG AGCCGGGGCC ACATGGTGTC CAGGGTCCAA TCGGCCCACC TGGTGAAGAA
    GGCAAACGAG GCCCCCGAGG TGATCCGGGC ACAGTTGGTC CTCCGGGCCC AGTGGGAGAA
    AGGGGTATTC CTTTTTTTCC TGACGTGTTC TACTTTGCTT TGAAACAGGG CGCTCAAGGA
    GAGAGGGGTC CTGTTGGCTC TTCAGGACCT AAAGGAGGTC AGGGGGATCC AGGACGTCCA
    GGTGAACCTG GGCTTCCAGG TGCTCGGGGT CTGACAGGAA ATCCTGGTGT CCAGGGTCCT
    GAAGGAAAAC TTGGACCTTT GGGTGCTCCA GGAGAAGACG GCCGGCCGGG TCCTCCAGGC
    TCCATAGGAA TCAGAGGGCA GCCTGGGAGT ATGGGTCTTC CAGGCCCCAA AGGTAGCAGT
    GGTGATCCTG GAAAACCTGG AGAAGCAGGA AATGCTGGTG TTCCTGGGCA GAGGGGAGCT
    CCTGGAAAAG ATGGCGAAGT TGGTCCTTCT GGTCCTGTGG GGCCCCCGGG TCTAGCCGGG
    GAGAGAGGAG AACAGGGTCC TCCAGGCCCC ACTGGCTTTC AGGGGCTTCC TGGTCCTCCA
    GGGCCTCCTG GGGAAGCTGG AAAGCCAGGT GATCAGGGGG TTCCTGGAGA TCCTGGGGCA
    GTTGGCCCAC TAGGACCTAG AGGAGAACGA GGAAATCCAG GGGAAAGAGG AGAACCAGGA
    ATAACTGGAC TTCCTGGTGA GAAAGGAATG GCTGGAGGAC ATGGTCCTGA TGGTCCAAAA
    GGCAGTCCAG GACCACCTGG AACCCCTGGA GATACAGGCC CACCAGGGCT TCAAGGTATG
    CCAGGAGAAA GGGGAATTGC AGGAACTCCT GGCCCCAAGG GTGACAGAGG TGGCATAGGA
    GAAAAAGGTG CTGAAGGTAC AGCTGGAAAT GATGGAGCAA GAGGTCTTCC GGGTCCCTTG
    GGCCCTCCAG GTCCTTCAGG CCCTACTGGA GAAAAGGGAG AACCCGGCCC TCGAGGTTTA
    GTCGGCCCGC CTGGCTCCCG TGGCAATCCC GGCTCACGTG GTGAAAATGG CCCAACTGGA
    GCTGTGGGTT TTGCGGGACC CCAGGGGCCG GATGGGCAGC CTGGAGTGAA AGGTGAACCT
    GGAGAGCCTG GGCAGAAGGG AGACGCTGGC TCTCCTGGAC CACAAGGACT GGCAGGATCC
    CCAGGGCCTC ATGGTCCTAA TGGTGTTCCC GGACTGAAAG GTGGTCGAGG AACTCAGGGT
    CCACCTGGTG CTACAGGATT TCCTGGTTCT GCTGGCAGAG TTGGACCTCC AGGTCCTGCT
    GGAGCTCCAG GACCTGCGGG GCCCTTAGGG GAGCCCGGGA AGGAAGGACC TCCAGGTCTT
    CGTGGGGACC CCGGGTCTCA TGGACGAGTA GGAGATCGAG GACCAGCTGG CCCTCCTGGT
    GGCCCAGGAG ACAAAGGGGA CCCAGGAGAA GATGGACAAC CTGGTCCAGA TGGTCCCCCT
    GGTCCAGCTG GAACTACTGG GCAAAGAGGA ATCGTCGGCA TGCCGGGGCA GCGTGGGGAG
    AGAGGGATGC CCGGCCTTCC AGGCCCAGCA GGCACACCAG GAAAAGTAGG ACCAACTGGT
    GCCCCAGGAG ATAAAGGTCC ACCTGGACCT GTTGGGCCCC CGGGCTCGAA TGGCCCTGTG
    GGGGAACCAG GACCGGAGGG TCCAGCTGGT AATGATGGTA CTCCAGGACG GGATGGTGCT
    GTTGGAGAAC GTGGTGATCG TGGAGACCCT GGGCCTGCAG GTCTGCCAGG CTCTCAGGGT
    GCCCCTGGAA CTCCTGGTCC TGTTGGTGCT CCGGGAGATG CAGGACAAAG AGGAGACCCG
    GGTTCTCGAG GTCCTATAGG ACCACCTGGT CGAGCTGGGA AACGTGGTTT ACTTGTAACA
    AGGGGTCCAA CTGGTGAACA AGGCAGTGCT GGAATCCCTG GACCATTTGG CCCAAGAGGA
    CCCCCGGGTC CAGTTGGTCC TTCAGGCAAA GAAGGAAGCC CTGGGCCACT TGGGCCCATT
    GGGCCTCCTG GTGTGCGGGG CAGCGTTGGA GAAGCAGGCC CTGAGGGTCC TCCTGGTGAG
    CCTGGTCCCC CTGGCCCTCC GGGACCCCCT GGCCACCTTA CAGCTGCTCT TGGGGACATC
    ATGGGGCACT ATGATGACAG CATGCCAGAC CCACTTCCGG AGTTTACTGA AGATCAGGCG
    GCTCCTGATG ACAAAAACAA AACGGACCCG GGGGTACATG CGACCCTGAA GTCACTCAGT
    AGTCAGATTG AAACCATGCG CAGCCCTGAT GGCTCTAGAA AGCACCCTGC CCGGACTTGT
    GATGATTTAA AGCTTTGCCA TTCTGCAAAG AAGAGCGGAG AGTACTGGAT TGACCCTAAC
    CAGGGATCTG CTGAAGATGC AATCAAAGTT TACTGCAACA TGGAAACAGG AGAAACATGT
    ATTTCAGCAA ATCCATCCAG TGTACCACGT AAAACCTGGT GGGCCAGCAA ATCTCCTGAT
    AATAAGCCTG TTTGGTATGG TCTTGATATG AATCGAGGAT CTCAGTTTGT TTATGGAGAC
    CACCAGTCAC CTAATGCAGC CATTACTCAG ATGACCTTCT TGCGCCTTTT ATCGAAAGAA
    GCCTCCCAGA ACATCACCTA CATCTGTAAA AACAGTGTAG GATACATGGA TGATCAAGCT
    AAGAACTTGA AGAAAGCTGT GGTTCTCAAA GGGTCAAATG ACTTAGAAAT CAAAGCAGAG
    GGAAACGTTA GATTCAGATA CATAGTTCTT CATGATTCTT GCTCTAAACG AAATGGAAAC
    GTGGGCAAGA CCATCTTTGA ATATAGAACG CAGAATGTGG CACGCTTGCC CATCATAGAT
    CTTGCCCCTG TGGATGTTGG CAGTACAGAC CAAGAATTTG GCGTTGAAAT TGGGCCAGTT
    TGTTTTGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89500
    Clone ID Related Accession (Same CDS sequence) XM_015462240.1
    Accession Version XM_015462240.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: collagen alpha-2(V) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644570.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATGGCAA ACTGGGTGGA AGCAAGACCT CTGCTCATTC TTACTGTTTT ATTAGGGCAG 
    TTCGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC
    TGCACCCAGA ATGGTCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGCCAG
    ATCTGTGTCT GTGACAATGG CGCCATTCTT TGTGACAAGA TTCAGTGCCA GGATGTGCTT
    GAGTGTGCTG ACCCCGTAAC TCCCCCTGGG GAATGCTGTC CTGTCTGTCC ACATACAACT
    GGAGGTGGCA ATACCAACTT TGGAAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA
    GTACCTGTTG TGACAGGCAT ACGTGGACGT CCAGGACCTG CAGGACCACC AGGATCACAA
    GGACCGAGAG GAGAGAGAGG CCCCAAAGGA AAACCTGGGC CTCGTGGTCC TCAGGGAATC
    GATGGAGAGC CAGGTGTTCC TGGTCAGCCA GGTGCCCCAG GACCTCCTGG GCATCCCTCT
    CACCCAGGAC CGGATGGCAT GAGCAGGCCG TTTTCAGCTC AGATGGCTGG GTTAGATGAG
    AAGTCTGGAC TTGGAAGTCA AGTAGGACTG ATGCCTGGAT CCGTGGGTCC TGTTGGCCCA
    AGGGGTCCTC AAGGTTTACA AGGGCAGCAG GGTGGTGTTG GCCCAGCAGG ACCTCCTGGT
    GAACCTGGTG ACCCTGGCCC AATGGGTCCA GTTGGTGCAC GAGGACCAGA GGGCCCTCCT
    GGAAAACCTG GTGAAGATGG TGAGCCTGGT AGAAATGGAA AACCTGGTGA AGTGGGATTT
    GCAGGATCTC CGGGAGCTCG AGGATTTCCT GGGGCTCCTG GTCTTCCAGG CCTGAAGGGT
    CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCAAC TGGTTCCAAG
    GGTGAAGCTG GCCCTACTGG TCCAATGGGT GCCATGGGTC CGATGGGCCC AAGAGGAATG
    CCAGGAGAGA GAGGAAGGCT TGGACCACAG GGTGCTCCTG GACAACGAGG TGCGCATGGT
    ATGCCTGGAA AACCTGGGCC CATGGGTCCT CTTGGGATAC CTGGCTCTGC TGGTTTTCCA
    GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCCACTGGGG CGCGAGGCCC TGAAGGTCCT
    CAAGGGCAAA GAGGGGAAAC CGGGCCCCCT GGTCCTGTTG GCTCCCAAGG TCTTCCTGGT
    GCAGTAGGAA CTGATGGTAC GCCTGGTGCC AAAGGCCCAA CGGGCTCTCC GGGTACTTCT
    GGTCCTCCTG GCTTAGCAGG ACCCCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGACCT
    CCCGGAATTC GAGGCCAACC GGGTGATCCA GGAGTCCCAG GTTTCAAAGG AGAAGCTGGA
    CCAAAAGGGG AGCCGGGGCC ACATGGTGTC CAGGGTCCAA TCGGCCCACC TGGTGAAGAA
    GGCAAACGAG GCCCCCGAGG TGATCCGGGC ACAGTTGGTC CTCCGGGCCC AGTGGGAGAA
    AGGGGGTGCT CCTGCAATCG TGGCTTTCCA GGCTCTGATG GTTTACCTGG ACACAAGGGC
    GCTCAAGGAG AGAGGGGTCC TGTTGGCTCT TCAGGACCTA AAGGAGGTCA GGGGGATCCA
    GGACGTCCAG GTGAACCTGG GCTTCCAGGT GCTCGGGGTC TGACAGGAAA TCCTGGTGTC
    CAGGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCTCCAG GAGAAGACGG CCGGCCGGGT
    CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCTGGGAGTA TGGGTCTTCC AGGCCCCAAA
    GGTAGCAGTG GTGATCCTGG AAAACCTGGA GAAGCAGGAA ATGCTGGTGT TCCTGGGCAG
    AGGGGAGCTC CTGGAAAAGA TGGCGAAGTT GGTCCTTCTG GTCCTGTGGG GCCCCCGGGT
    CTAGCCGGGG AGAGAGGAGA ACAGGGTCCT CCAGGCCCCA CTGGCTTTCA GGGGCTTCCT
    GGTCCTCCAG GGCCTCCTGG GGAAGCTGGA AAGCCAGGTG ATCAGGGGGT TCCTGGAGAT
    CCTGGGGCAG TTGGCCCACT AGGACCTAGA GGAGAACGAG GAAATCCAGG GGAAAGAGGA
    GAACCAGGAA TAACTGGACT TCCTGGTGAG AAAGGAATGG CTGGAGGACA TGGTCCTGAT
    GGTCCAAAAG GCAGTCCAGG ACCACCTGGA ACCCCTGGAG ATACAGGCCC ACCAGGGCTT
    CAAGGTATGC CAGGAGAAAG GGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT
    GGCATAGGAG AAAAAGGTGC TGAAGGTACA GCTGGAAATG ATGGAGCAAG AGGTCTTCCG
    GGTCCCTTGG GCCCTCCAGG TCCTTCAGGC CCTACTGGAG AAAAGGGAGA ACCCGGCCCT
    CGAGGTTTAG TCGGCCCGCC TGGCTCCCGT GGCAATCCCG GCTCACGTGG TGAAAATGGC
    CCAACTGGAG CTGTGGGTTT TGCGGGACCC CAGGGGCCGG ATGGGCAGCC TGGAGTGAAA
    GGTGAACCTG GAGAGCCTGG GCAGAAGGGA GACGCTGGCT CTCCTGGACC ACAAGGACTG
    GCAGGATCCC CAGGGCCTCA TGGTCCTAAT GGTGTTCCCG GACTGAAAGG TGGTCGAGGA
    ACTCAGGGTC CACCTGGTGC TACAGGATTT CCTGGTTCTG CTGGCAGAGT TGGACCTCCA
    GGTCCTGCTG GAGCTCCAGG ACCTGCGGGG CCCTTAGGGG AGCCCGGGAA GGAAGGACCT
    CCAGGTCTTC GTGGGGACCC CGGGTCTCAT GGACGAGTAG GAGATCGAGG ACCAGCTGGC
    CCTCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGACAACC TGGTCCAGAT
    GGTCCCCCTG GTCCAGCTGG AACTACTGGG CAAAGAGGAA TCGTCGGCAT GCCGGGGCAG
    CGTGGGGAGA GAGGGATGCC CGGCCTTCCA GGCCCAGCAG GCACACCAGG AAAAGTAGGA
    CCAACTGGTG CCCCAGGAGA TAAAGGTCCA CCTGGACCTG TTGGGCCCCC GGGCTCGAAT
    GGCCCTGTGG GGGAACCAGG ACCGGAGGGT CCAGCTGGTA ATGATGGTAC TCCAGGACGG
    GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC
    TCTCAGGGTG CCCCTGGAAC TCCTGGTCCT GTTGGTGCTC CGGGAGATGC AGGACAAAGA
    GGAGACCCGG GTTCTCGAGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGTTTA
    CTTGGACCGC AAGGACCTCG TGGTGACAAA GGCGATCATG GAGACAGAGG TGATCGAGGT
    CAGAAGGGTC ACAGAGGCTT TACTGGTNCT CAGGGTCTTC CTGGACCTCC GTAAGGTCCA
    ACTGGTGAAC AAGGCAGTGC TGGAATCCCT GGACCATTTG GCCCAAGAGG ACCCCCGGGT
    CCAGTTGGTC CTTCAGGCAA AGAAGGAAGC CCTGGGCCAC TTGGGCCCAT TGGGCCTCCT
    GGTGTGCGGG GCAGCGTTGG AGAAGCAGGC CCTGAGGGTC CTCCTGGTGA GCCTGGTCCC
    CCTGGCCCTC CGGGACCCCC TGGCCACCTT ACAGCTGCTC TTGGGGACAT CATGGGGCAC
    TATGATGACA GCATGCCAGA CCCACTTCCG GAGTTTACTG AAGATCAGGC GGCTCCTGAT
    GACAAAAACA AAACGGACCC GGGGGTACAT GCGACCCTGA AGTCACTCAG TAGTCAGATT
    GAAACCATGC GCAGCCCTGA TGGCTCTAGA AAGCACCCTG CCCGGACTTG TGATGATTTA
    AAGCTTTGCC ATTCTGCAAA GAAGAGCGGA GAGTACTGGA TTGACCCTAA CCAGGGATCT
    GCTGAAGATG CAATCAAAGT TTACTGCAAC ATGGAAACAG GAGAAACATG TATTTCAGCA
    AATCCATCCA GTGTACCACG TAAAACCTGG TGGGCCAGCA AATCTCCTGA TAATAAGCCT
    GTTTGGTATG GTCTTGATAT GAATCGAGGA TCTCAGTTTG TTTATGGAGA CCACCAGTCA
    CCTAATGCAG CCATTACTCA GATGACCTTC TTGCGCCTTT TATCGAAAGA AGCCTCCCAG
    AACATCACCT ACATCTGTAA AAACAGTGTA GGATACATGG ATGATCAAGC TAAGAACTTG
    AAGAAAGCTG TGGTTCTCAA AGGGTCAAAT GACTTAGAAA TCAAAGCAGA GGGAAACGTT
    AGATTCAGAT ACATAGTTCT TCATGATTCT TGCTCTAAAC GAAATGGAAA CGTGGGCAAG
    ACCATCTTTG AATATAGAAC GCAGAATGTG GCACGCTTGC CCATCATAGA TCTTGCCCCT
    GTGGATGTTG GCAGTACAGA CCAAGAATTT GGCGTTGAAA TTGGGCCAGT TTGTTTTGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317726.1
    CDS4..4506
    Translation

    Target ORF information:

    RefSeq Version XM_015462240.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 2 (COL5A2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462240.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATGGCAA ACTGGGTGGA AGCAAGACCT CTGCTCATTC TTACTGTTTT ATTAGGGCAG 
    TTCGTCTCAA TAAAAGCCCA GGAAGAAGAC GAGGATGAAG GATATGGTGA AGAAATAGCC
    TGCACCCAGA ATGGTCAGAT GTACTTAAAC AGGGACATTT GGAAACCTGC CCCTTGCCAG
    ATCTGTGTCT GTGACAATGG CGCCATTCTT TGTGACAAGA TTCAGTGCCA GGATGTGCTT
    GAGTGTGCTG ACCCCGTAAC TCCCCCTGGG GAATGCTGTC CTGTCTGTCC ACATACAACT
    GGAGGTGGCA ATACCAACTT TGGAAGAGGA AGAAAGGGAC AAAAGGGAGA ACCAGGATTA
    GTACCTGTTG TGACAGGCAT ACGTGGACGT CCAGGACCTG CAGGACCACC AGGATCACAA
    GGACCGAGAG GAGAGAGAGG CCCCAAAGGA AAACCTGGGC CTCGTGGTCC TCAGGGAATC
    GATGGAGAGC CAGGTGTTCC TGGTCAGCCA GGTGCCCCAG GACCTCCTGG GCATCCCTCT
    CACCCAGGAC CGGATGGCAT GAGCAGGCCG TTTTCAGCTC AGATGGCTGG GTTAGATGAG
    AAGTCTGGAC TTGGAAGTCA AGTAGGACTG ATGCCTGGAT CCGTGGGTCC TGTTGGCCCA
    AGGGGTCCTC AAGGTTTACA AGGGCAGCAG GGTGGTGTTG GCCCAGCAGG ACCTCCTGGT
    GAACCTGGTG ACCCTGGCCC AATGGGTCCA GTTGGTGCAC GAGGACCAGA GGGCCCTCCT
    GGAAAACCTG GTGAAGATGG TGAGCCTGGT AGAAATGGAA AACCTGGTGA AGTGGGATTT
    GCAGGATCTC CGGGAGCTCG AGGATTTCCT GGGGCTCCTG GTCTTCCAGG CCTGAAGGGT
    CACCGAGGAC ACAAAGGTCT TGAAGGCCCT AAAGGTGAAG TTGGAGCAAC TGGTTCCAAG
    GGTGAAGCTG GCCCTACTGG TCCAATGGGT GCCATGGGTC CGATGGGCCC AAGAGGAATG
    CCAGGAGAGA GAGGAAGGCT TGGACCACAG GGTGCTCCTG GACAACGAGG TGCGCATGGT
    ATGCCTGGAA AACCTGGGCC CATGGGTCCT CTTGGGATAC CTGGCTCTGC TGGTTTTCCA
    GGAAATCCTG GAATGAAGGG AGAAGCAGGT CCCACTGGGG CGCGAGGCCC TGAAGGTCCT
    CAAGGGCAAA GAGGGGAAAC CGGGCCCCCT GGTCCTGTTG GCTCCCAAGG TCTTCCTGGT
    GCAGTAGGAA CTGATGGTAC GCCTGGTGCC AAAGGCCCAA CGGGCTCTCC GGGTACTTCT
    GGTCCTCCTG GCTTAGCAGG ACCCCCTGGA TCTCCAGGAC CTCAGGGTAG CACTGGACCT
    CCCGGAATTC GAGGCCAACC GGGTGATCCA GGAGTCCCAG GTTTCAAAGG AGAAGCTGGA
    CCAAAAGGGG AGCCGGGGCC ACATGGTGTC CAGGGTCCAA TCGGCCCACC TGGTGAAGAA
    GGCAAACGAG GCCCCCGAGG TGATCCGGGC ACAGTTGGTC CTCCGGGCCC AGTGGGAGAA
    AGGGGGTGCT CCTGCAATCG TGGCTTTCCA GGCTCTGATG GTTTACCTGG ACACAAGGGC
    GCTCAAGGAG AGAGGGGTCC TGTTGGCTCT TCAGGACCTA AAGGAGGTCA GGGGGATCCA
    GGACGTCCAG GTGAACCTGG GCTTCCAGGT GCTCGGGGTC TGACAGGAAA TCCTGGTGTC
    CAGGGTCCTG AAGGAAAACT TGGACCTTTG GGTGCTCCAG GAGAAGACGG CCGGCCGGGT
    CCTCCAGGCT CCATAGGAAT CAGAGGGCAG CCTGGGAGTA TGGGTCTTCC AGGCCCCAAA
    GGTAGCAGTG GTGATCCTGG AAAACCTGGA GAAGCAGGAA ATGCTGGTGT TCCTGGGCAG
    AGGGGAGCTC CTGGAAAAGA TGGCGAAGTT GGTCCTTCTG GTCCTGTGGG GCCCCCGGGT
    CTAGCCGGGG AGAGAGGAGA ACAGGGTCCT CCAGGCCCCA CTGGCTTTCA GGGGCTTCCT
    GGTCCTCCAG GGCCTCCTGG GGAAGCTGGA AAGCCAGGTG ATCAGGGGGT TCCTGGAGAT
    CCTGGGGCAG TTGGCCCACT AGGACCTAGA GGAGAACGAG GAAATCCAGG GGAAAGAGGA
    GAACCAGGAA TAACTGGACT TCCTGGTGAG AAAGGAATGG CTGGAGGACA TGGTCCTGAT
    GGTCCAAAAG GCAGTCCAGG ACCACCTGGA ACCCCTGGAG ATACAGGCCC ACCAGGGCTT
    CAAGGTATGC CAGGAGAAAG GGGAATTGCA GGAACTCCTG GCCCCAAGGG TGACAGAGGT
    GGCATAGGAG AAAAAGGTGC TGAAGGTACA GCTGGAAATG ATGGAGCAAG AGGTCTTCCG
    GGTCCCTTGG GCCCTCCAGG TCCTTCAGGC CCTACTGGAG AAAAGGGAGA ACCCGGCCCT
    CGAGGTTTAG TCGGCCCGCC TGGCTCCCGT GGCAATCCCG GCTCACGTGG TGAAAATGGC
    CCAACTGGAG CTGTGGGTTT TGCGGGACCC CAGGGGCCGG ATGGGCAGCC TGGAGTGAAA
    GGTGAACCTG GAGAGCCTGG GCAGAAGGGA GACGCTGGCT CTCCTGGACC ACAAGGACTG
    GCAGGATCCC CAGGGCCTCA TGGTCCTAAT GGTGTTCCCG GACTGAAAGG TGGTCGAGGA
    ACTCAGGGTC CACCTGGTGC TACAGGATTT CCTGGTTCTG CTGGCAGAGT TGGACCTCCA
    GGTCCTGCTG GAGCTCCAGG ACCTGCGGGG CCCTTAGGGG AGCCCGGGAA GGAAGGACCT
    CCAGGTCTTC GTGGGGACCC CGGGTCTCAT GGACGAGTAG GAGATCGAGG ACCAGCTGGC
    CCTCCTGGTG GCCCAGGAGA CAAAGGGGAC CCAGGAGAAG ATGGACAACC TGGTCCAGAT
    GGTCCCCCTG GTCCAGCTGG AACTACTGGG CAAAGAGGAA TCGTCGGCAT GCCGGGGCAG
    CGTGGGGAGA GAGGGATGCC CGGCCTTCCA GGCCCAGCAG GCACACCAGG AAAAGTAGGA
    CCAACTGGTG CCCCAGGAGA TAAAGGTCCA CCTGGACCTG TTGGGCCCCC GGGCTCGAAT
    GGCCCTGTGG GGGAACCAGG ACCGGAGGGT CCAGCTGGTA ATGATGGTAC TCCAGGACGG
    GATGGTGCTG TTGGAGAACG TGGTGATCGT GGAGACCCTG GGCCTGCAGG TCTGCCAGGC
    TCTCAGGGTG CCCCTGGAAC TCCTGGTCCT GTTGGTGCTC CGGGAGATGC AGGACAAAGA
    GGAGACCCGG GTTCTCGAGG TCCTATAGGA CCACCTGGTC GAGCTGGGAA ACGTGGTTTA
    CTTGGACCGC AAGGACCTCG TGGTGACAAA GGCGATCATG GAGACAGAGG TGATCGAGGT
    CAGAAGGGTC ACAGAGGCTT TACTGGTNCT CAGGGTCTTC CTGGACCTCC GTAAGGTCCA
    ACTGGTGAAC AAGGCAGTGC TGGAATCCCT GGACCATTTG GCCCAAGAGG ACCCCCGGGT
    CCAGTTGGTC CTTCAGGCAA AGAAGGAAGC CCTGGGCCAC TTGGGCCCAT TGGGCCTCCT
    GGTGTGCGGG GCAGCGTTGG AGAAGCAGGC CCTGAGGGTC CTCCTGGTGA GCCTGGTCCC
    CCTGGCCCTC CGGGACCCCC TGGCCACCTT ACAGCTGCTC TTGGGGACAT CATGGGGCAC
    TATGATGACA GCATGCCAGA CCCACTTCCG GAGTTTACTG AAGATCAGGC GGCTCCTGAT
    GACAAAAACA AAACGGACCC GGGGGTACAT GCGACCCTGA AGTCACTCAG TAGTCAGATT
    GAAACCATGC GCAGCCCTGA TGGCTCTAGA AAGCACCCTG CCCGGACTTG TGATGATTTA
    AAGCTTTGCC ATTCTGCAAA GAAGAGCGGA GAGTACTGGA TTGACCCTAA CCAGGGATCT
    GCTGAAGATG CAATCAAAGT TTACTGCAAC ATGGAAACAG GAGAAACATG TATTTCAGCA
    AATCCATCCA GTGTACCACG TAAAACCTGG TGGGCCAGCA AATCTCCTGA TAATAAGCCT
    GTTTGGTATG GTCTTGATAT GAATCGAGGA TCTCAGTTTG TTTATGGAGA CCACCAGTCA
    CCTAATGCAG CCATTACTCA GATGACCTTC TTGCGCCTTT TATCGAAAGA AGCCTCCCAG
    AACATCACCT ACATCTGTAA AAACAGTGTA GGATACATGG ATGATCAAGC TAAGAACTTG
    AAGAAAGCTG TGGTTCTCAA AGGGTCAAAT GACTTAGAAA TCAAAGCAGA GGGAAACGTT
    AGATTCAGAT ACATAGTTCT TCATGATTCT TGCTCTAAAC GAAATGGAAA CGTGGGCAAG
    ACCATCTTTG AATATAGAAC GCAGAATGTG GCACGCTTGC CCATCATAGA TCTTGCCCCT
    GTGGATGTTG GCAGTACAGA CCAAGAATTT GGCGTTGAAA TTGGGCCAGT TTGTTTTGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.