ARHGAP21 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005194286.3	XP_005194343.1	rho GTPase-activating protein 21 isoform X1
XM_005214298.3	XP_005214355.1	rho GTPase-activating protein 21 isoform X1
XM_025000959.1	XP_024856727.1	rho GTPase-activating protein 21 isoform X2
XM_025000962.1	XP_024856730.1	rho GTPase-activating protein 21 isoform X5
XM_005194293.3	XP_005194350.1	rho GTPase-activating protein 21 isoform X5
XM_010822424.2	XP_010820726.1	rho GTPase-activating protein 21 isoform X4
XM_025000958.1	XP_024856726.1	rho GTPase-activating protein 21 isoform X1
XM_005214297.4	XP_005214354.1	rho GTPase-activating protein 21 isoform X5
XM_025000963.1	XP_024856731.1	rho GTPase-activating protein 21 isoform X6
XM_005214297.3	XP_005214354.1	rho GTPase-activating protein 21 isoform X1
XM_005214301.3	XP_005214358.1	rho GTPase-activating protein 21 isoform X2
XM_005194287.3	XP_005194344.1	rho GTPase-activating protein 21 isoform X1
XM_005214298.4	XP_005214355.1	rho GTPase-activating protein 21 isoform X5
XM_010811198.2	XP_010809500.1	rho GTPase-activating protein 21 isoform X8
XM_015465939.1	XP_015321425.1	rho GTPase-activating protein 21 isoform X1
XM_005194291.3	XP_005194348.1	rho GTPase-activating protein 21 isoform X3
XM_010822425.2	XP_010820727.1	rho GTPase-activating protein 21 isoform X6
XM_025000961.1	XP_024856729.1	rho GTPase-activating protein 21 isoform X4
XM_015474021.2	XP_015329507.1	rho GTPase-activating protein 21 isoform X5
XM_015474021.1	XP_015329507.1	rho GTPase-activating protein 21 isoform X1
XM_005214302.3	XP_005214359.1	rho GTPase-activating protein 21 isoform X3
XM_010811197.2	XP_010809499.1	rho GTPase-activating protein 21 isoform X4
XM_005194290.3	XP_005194347.1	rho GTPase-activating protein 21 isoform X2
XM_025000960.1	XP_024856728.1	rho GTPase-activating protein 21 isoform X3
XM_005214304.3	XP_005214361.1	rho GTPase-activating protein 21 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa221856	XM_025000958.1 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221857	XM_025000959.1 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221858	XM_025000960.1 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221859	XM_025000961.1 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa07464	XM_015465939.1 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07464	XM_005194287.3 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07464	XM_025000962.1 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07464	XM_015474021.2 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07464	XM_005194286.3 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07464	XM_005214297.4 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07464	XM_005214298.4 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07465	XM_005214301.3 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07465	XM_005194290.3 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07466	XM_005214302.3 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07466	XM_025000963.1 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07466	XM_005194291.3 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07469	XM_005194293.3 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07469	XM_005214304.3 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa53931	XM_010822424.2 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa53931	XM_010811197.2 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa53932	XM_010822425.2 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa53932	XM_010811198.2 Latest version!	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OBa07464	XM_005214297.3	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07464	XM_005214298.3	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa07464	XM_015474021.1	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

ARHGAP21 ( XM_025000958.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856726.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa221856
Clone ID Related Accession (Same CDS sequence)	XM_025000958.1
Accession Version	XM_025000958.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGGA GCCTCGACAC ATGTAGAGAG AGAGCGTCTG AAGGGTCCTC TCAGTGGAGG 
CATCTGGCTG CTGTGCCCTT CGCTGTGTGC CTGCAGTTTG CCCTTGGTGT AAAATCCGGC 
AGATCAAAGG CTCTGCGGGT ATCAAAAAGT AAAGATGGAA AAGAACAAAG TGAAACTGTA 
TCACCATCCG AAGATGAAAT GTTCTCCTGG CCAGGTCCCA AAACAGTTAT GTTGAAAAGA 
ACATCTCAAG GCTTTGGTTT TACATTGAGG CATTTTATTG TTTATCCCCC AGAGTCTGCA 
ATTCAGTTTT CATTTAAGGA TGAAGAAAAT GGCAACAGAG GAGGAAAACA AAGAAACCGT 
TTGGAACCCA TGGACACCAT CTTTGTTAAA CAGGTGAAAG AGGGCGGACC TGCTTTTGAA 
GCTGGATTAT GCACAGGTGA TCGAATTATA AAAGTCAATG GAGAAAGTGT TATTGGAAAG 
ACCTATTCTC AAGTAATTGC TTTAATCCAG AACAGTGATA CAACATTGGA ACTTAGTGTT 
ATGCCAAAAG ATGAAGACAT TCTCCAAGTG CTACAATTTA CAAAGGATGT CACAGCTCTG 
GCGTATTCTC AAGATGCCTA CCTGAAAGGC AATGAAGCCT ACAGCGGCAA TGCCCGCAAC 
ATCCCTGAGC CCCCACCAGT CTGCTACCCC TGGCCGTCAT CCGCCCCTCC CGCCATGGCG 
CAGCCCCCTG AACCACCTCC TCCAGACTCC ACAGCAAGCA AACCGCAGAC TAGCCCCCCA 
GTATTGACGC AGCCCGGCAG GGCATACAGG ATGGAAATCC AAGTGCCTCC GTCACCACCA 
GAGGTGGCCA AGTCCAACAC AGCAGTGTGT GTCTGCAATG AGAGTGTGAG GACCGTCATT 
GTACCTTCTG AGAAGGTTGT AGATTTATTA CCTAACAGAA GCAACCACAC AGTTCCCGCT 
CACAGAACTG AGGAAGTGAG GTATGGCCGA AACGAACAGC CCTCTTTCAA AACAGCGACG 
AGAACCACCT CACCGCCATC CTCAGTTCCC ACTGCCCATC TCATCCACCA GCCCGGCTCC 
AGGTCGTTGG AACCTTCTGG CATTTTACTT AAATCTGGCC ACTACGGCGG ACACTCAGAA 
GGTCTGGTGA GCAGATCTCC AGCTGTGGAG TCTCCTTCCG TAACTGTCAA CCACTACTCT 
CCCAACTCCC ATCAGCACAT AGACTGGAAA AACTATAAAA CTTACAAAGA GTATATTGAT 
AACCGGCGCT TGCACATGGG CTGTCGGACC ATTCAAGAAA GGTTAGATAG TTTAAGAGCC 
GCCTCTCAGA GCACAGCGGA TTATAACCAG GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA 
CGGCGTCGGA GCACCTCTCA GGACCGAGTG CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC 
GTGTCCCAGG AGAGGCTGGA GGACTCTGTG TTGATGATGT ATTGTCCGAG GAGTGCCTCT 
CAAGGTGCGC TGACATCCCC TTCTATTAGT TTTAGTAACC ACAGAACTCG CTCGTGGGAT 
TACATCGAGG GACAGGGTGA AACCTCGGAA AACGTCCATT CTGAATGTCC ACCGCCGGAT 
GCAAATGGTG AACGAAAGCA GACTTACAAG TGGAGTGGGT TCACCGAACA GGACGATCGT 
CGAGGTATTT ACGAGAGACC CAGGCAGCAA GAAATTCACA AATCTTTCCG AGGCTCCAAT 
TTTACAGTGG CTCCCAGTGT GGTGAATTCT GACAGCAGGA GAGTGAGCAG CAGAATCGGG 
GGATCAGTGT CTCAGTTTAA AAAAATTCCA GCAGATCTAA AACCCCTGCA GCCCAGCAGA 
AATTTTCAAA CTGCTTGTGG AATATCCCAG CCTCGAGGGA TTTCCCAAGA CAGGTCACCT 
CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA GGGCCTTCTA TGCATGTCGC GAAACCGCCC 
TCCAGCCAGA ACTCATTTCT TTCTATCAAA GACCAAAGAC CAGTAAATCA CTTGCATCAA 
AACAGTTTAT TGAATCAGCA GCCATGGTTA CGGACGGAAA GTGCCCCCGA TCACCAAGTG 
GACACTGGGA AGCCCCCTTC TCTTTCCGGA GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT 
GAGAATGCTA GCACTCCAGA TTTTGAGTTA TCCGTCTCTC AGAGGAATCA AGATTTAAAT 
GTACAAGAGG TGGAAATTCA GCAATCGAAT GCTGTAGATA ATAAAGAAAC TGTCATCCTA 
AGAGAAAAAC CTCCGTCTGG TCGCCAGACA CCGCAGCCTT TAAGGCATCA GTCTTACATC 
TTAGCAGTGA GTGACCAGGA GACAGGCTCA GACACCACCT GCTGGCTGCC TAATGATGCT 
CGCCGGGAGG TCCATATAAA AAGGATGGAG GAGAGGAAGG CCTCAAGTAC CACTCCACCT 
GGTGATTCCT TGGCTTCCAT CCCATTTATA GATGAACCTA CCAGCCCTAG CATCGATCAC 
GACATTTCTC ACATCCCCGC CTCTGCGGTC ATCTCTGCCT CCACCTCCCA GGTACCCTCC 
ATAGCAACGG TCCCTCCCAG CCTCACAACT TCAGCTCCAT TAATCCGACG TCAGCTCTCC 
CATGACCAGG AATCTGTTGG ACCTCCCAGC CTGGATGCCC AGCCCAGCTC AAAGACAGAG 
CGATCCAAAT CATATGATGA GGGCCTGGAT GATTACAGAG AAGATGCAAA ACTGTCTTTT 
AAACATGTAT CTAGCCTGAA GGGAATCAAG ATCTTAGACA GTCAAAAGTC ATCAGAAGAT 
TCCGGATCCA GGAAAGACTC TTCCTCAGAG GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG 
CTTCATTTCA GGCCACTCGT CACTGATAAG GGCAAGCGCG TTGGTGGAAG TATTCGGCCA 
TGGAAGCAGA TGTATGTTGT ACTTCGGGGC CATTCGCTGT ATCTGTACAA AGATAAACGA 
GAACAGACGA CTCCGTCTGA GGAGGAGCAG CCCATCAGTG TTAACGCCTG CTTAATAGAC 
ATCTCTTACA GCGAGACCAA GAGGAAGAAT GTCTTTCGAC TCACCACGTC TGACTGCGAG 
TGCCTGTTTC AGGCTGAAGA CAGGGATGAC ATGTTAGCCT GGATCAAAAC CATCCAGGAG 
AGCAGCAATT TAAACGAGGA GGACACTGGA GTCACTAACA GGGACCTAAT TAGTCGAAGA 
ATAAAAGAAT ACAACAGTCT AATGAGCAGC AAAGCAGAGC CATTGCCGAA GACCCCTCGC 
CAGAGTCTCA GCATCCGGCA GACCCTACTT GGTGCTAAAG CAGAGCCTCG GACTCAGAGT 
CCACACTCTC CCAAAGAGGA GTCAGAAAGG AAGCTTCTTA GTAAAGATGA TACCAGTCCT 
CCAAAAGACA AAGGCACGTG GAGAAAAGGC ATTCCAAGTA TTATGAGAAA GACATTTGAG 
AAAAAGCCTA CTGCTACGGG AACATTCGGG GTCAGACTGG ACGACTGCCC ACCAGCTCAT 
ACAAACCGGT ATATTCCATT AATAGTTGAC ATATGTTGTA AATTAGTTGA AGAAAGAGGT 
CTGGAATATA CAGGTATTTA CAGAGTCCCT GGAAACAATG CAGCCATCTC CAGTATGCAG 
GAGGAGCTCA ACAAGGGAAT GGCTGATATT GATATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATCA GCAGTTTATT AAAATCCTTC TTCAGAAAAC TCCCAGAACC TCTCTTCACG 
AATGATAAAT ACGCCGACTT TATTGAAGCC AATCGTAAGG AAGATCCTCT AGATCGTCTG 
AAAACATTAA AAAGACTAAT TCATGATTTG CCCGAACATC ATTATGAAAC ACTCAAGTTT 
CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA AATTCAGAAA AAAATAAGAT GGAACCAAGA 
AACCTAGCCA TAGTGTTTGG TCCAACTCTT GTGAGAACCT CCGAAGATAA CATGACACAC 
ATGGTCACTC ACATGCCAGA TCAGTACAAG ATCGTAGAGA CACTCATCCA GCACCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA CCTCTTACAA CAGTGCAGGA GGAAAACACA 
GTAGACTCCC AGCCAGTGCC AAACATAGAT CATTTACTCA CCAACATTGG AAGGACAGGC 
GTCTCCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CAACAAAATC TAAGGGTTCT 
TGGGGATCCG GAAAAGACCA GTACAGCAAG GACCTGCTTG TATCCTCCAT CTTCGCGGCT 
GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA AAAGCACAAC CCAGCAGCTC GGAAGACGAG 
CTGGACAATG TGTTTTTCAA GAAAGAAAAC TCAGAGCAGG GTCACCACGA CATTAAAGAA 
GAGTCCAAAA AAGAGAGTGA GACACCAGGC CGGAAACAAA GGACCGTTGC TCCCAAAGAA 
AACAACACGA AGAAAGACAG CAGCGGAGGT GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG 
GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC ACCAAGCAGA GCCGGTCACC CACCCTGAGC 
TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC AGGGCCCTCG CAGCCCAGAA GACCTCCCAC 
CTGGAAGACA CGGGGTCCGA CTCCAGCACC CCACTCAGCA CTCACGCCCC GGCTCCCCCG 
GCAAGCTTTC CCACCAAGAA ACCTCCCAGC CCCGAGCCCA AGCACAGCGA GCTCCTGGTC 
AGTGTCGGCA GCATCACCTC CGACCACGCC GGCACACCGT CTGCTCCCTA CCTGGCCGGC 
CTCGACTCCA GCCGGCTGAG CCCCGAGGTG CAGTCCATGG CCGAGAGCCG GGGATTTGAA 
GCTGATGACG AGAGGAGCGA GCTGGTCAGC GAGGGCCGGC CAGTGGAGAC AGACAGCGAG 
AGCGACTTTC CGGTCTTCCC CGCCGCCCCG GCCTCGGACA GGCTCTTCCG AGGAAAACTC 
CAAGAGGCCG CGAGGACCAG CCGGAGAAAC TCAGAAGGCA GTGAGGTCAG CTGCACGGAA 
GGAAGTTTAA CACCAAGTTT AGAAAGCCGG AGACAGCTCT TCAGCTCCCA TAAACTGATC 
GAGTGTGACA CTCTGTCCAG GAAGAAATCC GCCAGGTTCA AGTCAGACAG TGGGAGTCTA 
GGAGATGCCA AGAATGAGAA AGAAGCCCCT TCCATCACCA AAGTGTTCGA CGTTATGAAA 
AAAGGAAAAT CCACCGGAAA TTTACTGACA CCACCAGCCA GAAGCGAATC GGACAAACAG 
GAACCCACTT GGAAAACAAA AATAGCCGAT CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC 
GATGACATGT TTGGAGTAGG AAGTCAAAAA ACCATCGCCG AACCTGCCAA AAGGAAAAAC 
ATCAAACGCA GACACACGCT GGGTGGGCAC AGGGATGCTA CTGAAATGAG TGTTCTGAAT 
TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC AGAGAATCCG AACTTTCAGC TGTGAATCGA 
TTGAAGCCCA AATGCTCCGC CCAGGACCTG TCCATCTCAG ACTGGCTGGC CAGGGAGCGG 
CTACGCACCA GTATGACTGA CCTGAGCAGA GGGGACACGG GGGACCCCCG GCCTGAGAGT 
CTTGGCACCT TAGAAATACC AACCAGGGAC CCGCCCCTGT CTTTCCAGTC TGATGCAGAC 
AGTTCTTCCA GCACCTTGGC TTCAACTAAC AGGGCCCCTC TTTCCACACC ATCACAGTCA 
CCTGACCAAA TAAATGGAGA AAGCTTCCAG AACACGAGCC AAAACTCCAG TTCTGCAGCC 
AGGGTCCAAC CTCATCAACT GTCTGAAACC CCAGATCACA AAGCACAGTT CCATCCCTGT 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856726.1
CDS	74..6019
Translation	MVGSLDTCRERASEGSSQWRHLAAVPFAVCLQFALGVKSGRSKALRVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_025000958.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000958.1

ORF Insert Sequence:

ATGGTGGGGA GCCTCGACAC ATGTAGAGAG AGAGCGTCTG AAGGGTCCTC TCAGTGGAGG 
CATCTGGCTG CTGTGCCCTT CGCTGTGTGC CTGCAGTTTG CCCTTGGTGT AAAATCCGGC 
AGATCAAAGG CTCTGCGGGT ATCAAAAAGT AAAGATGGAA AAGAACAAAG TGAAACTGTA 
TCACCATCCG AAGATGAAAT GTTCTCCTGG CCAGGTCCCA AAACAGTTAT GTTGAAAAGA 
ACATCTCAAG GCTTTGGTTT TACATTGAGG CATTTTATTG TTTATCCCCC AGAGTCTGCA 
ATTCAGTTTT CATTTAAGGA TGAAGAAAAT GGCAACAGAG GAGGAAAACA AAGAAACCGT 
TTGGAACCCA TGGACACCAT CTTTGTTAAA CAGGTGAAAG AGGGCGGACC TGCTTTTGAA 
GCTGGATTAT GCACAGGTGA TCGAATTATA AAAGTCAATG GAGAAAGTGT TATTGGAAAG 
ACCTATTCTC AAGTAATTGC TTTAATCCAG AACAGTGATA CAACATTGGA ACTTAGTGTT 
ATGCCAAAAG ATGAAGACAT TCTCCAAGTG CTACAATTTA CAAAGGATGT CACAGCTCTG 
GCGTATTCTC AAGATGCCTA CCTGAAAGGC AATGAAGCCT ACAGCGGCAA TGCCCGCAAC 
ATCCCTGAGC CCCCACCAGT CTGCTACCCC TGGCCGTCAT CCGCCCCTCC CGCCATGGCG 
CAGCCCCCTG AACCACCTCC TCCAGACTCC ACAGCAAGCA AACCGCAGAC TAGCCCCCCA 
GTATTGACGC AGCCCGGCAG GGCATACAGG ATGGAAATCC AAGTGCCTCC GTCACCACCA 
GAGGTGGCCA AGTCCAACAC AGCAGTGTGT GTCTGCAATG AGAGTGTGAG GACCGTCATT 
GTACCTTCTG AGAAGGTTGT AGATTTATTA CCTAACAGAA GCAACCACAC AGTTCCCGCT 
CACAGAACTG AGGAAGTGAG GTATGGCCGA AACGAACAGC CCTCTTTCAA AACAGCGACG 
AGAACCACCT CACCGCCATC CTCAGTTCCC ACTGCCCATC TCATCCACCA GCCCGGCTCC 
AGGTCGTTGG AACCTTCTGG CATTTTACTT AAATCTGGCC ACTACGGCGG ACACTCAGAA 
GGTCTGGTGA GCAGATCTCC AGCTGTGGAG TCTCCTTCCG TAACTGTCAA CCACTACTCT 
CCCAACTCCC ATCAGCACAT AGACTGGAAA AACTATAAAA CTTACAAAGA GTATATTGAT 
AACCGGCGCT TGCACATGGG CTGTCGGACC ATTCAAGAAA GGTTAGATAG TTTAAGAGCC 
GCCTCTCAGA GCACAGCGGA TTATAACCAG GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA 
CGGCGTCGGA GCACCTCTCA GGACCGAGTG CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC 
GTGTCCCAGG AGAGGCTGGA GGACTCTGTG TTGATGATGT ATTGTCCGAG GAGTGCCTCT 
CAAGGTGCGC TGACATCCCC TTCTATTAGT TTTAGTAACC ACAGAACTCG CTCGTGGGAT 
TACATCGAGG GACAGGGTGA AACCTCGGAA AACGTCCATT CTGAATGTCC ACCGCCGGAT 
GCAAATGGTG AACGAAAGCA GACTTACAAG TGGAGTGGGT TCACCGAACA GGACGATCGT 
CGAGGTATTT ACGAGAGACC CAGGCAGCAA GAAATTCACA AATCTTTCCG AGGCTCCAAT 
TTTACAGTGG CTCCCAGTGT GGTGAATTCT GACAGCAGGA GAGTGAGCAG CAGAATCGGG 
GGATCAGTGT CTCAGTTTAA AAAAATTCCA GCAGATCTAA AACCCCTGCA GCCCAGCAGA 
AATTTTCAAA CTGCTTGTGG AATATCCCAG CCTCGAGGGA TTTCCCAAGA CAGGTCACCT 
CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA GGGCCTTCTA TGCATGTCGC GAAACCGCCC 
TCCAGCCAGA ACTCATTTCT TTCTATCAAA GACCAAAGAC CAGTAAATCA CTTGCATCAA 
AACAGTTTAT TGAATCAGCA GCCATGGTTA CGGACGGAAA GTGCCCCCGA TCACCAAGTG 
GACACTGGGA AGCCCCCTTC TCTTTCCGGA GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT 
GAGAATGCTA GCACTCCAGA TTTTGAGTTA TCCGTCTCTC AGAGGAATCA AGATTTAAAT 
GTACAAGAGG TGGAAATTCA GCAATCGAAT GCTGTAGATA ATAAAGAAAC TGTCATCCTA 
AGAGAAAAAC CTCCGTCTGG TCGCCAGACA CCGCAGCCTT TAAGGCATCA GTCTTACATC 
TTAGCAGTGA GTGACCAGGA GACAGGCTCA GACACCACCT GCTGGCTGCC TAATGATGCT 
CGCCGGGAGG TCCATATAAA AAGGATGGAG GAGAGGAAGG CCTCAAGTAC CACTCCACCT 
GGTGATTCCT TGGCTTCCAT CCCATTTATA GATGAACCTA CCAGCCCTAG CATCGATCAC 
GACATTTCTC ACATCCCCGC CTCTGCGGTC ATCTCTGCCT CCACCTCCCA GGTACCCTCC 
ATAGCAACGG TCCCTCCCAG CCTCACAACT TCAGCTCCAT TAATCCGACG TCAGCTCTCC 
CATGACCAGG AATCTGTTGG ACCTCCCAGC CTGGATGCCC AGCCCAGCTC AAAGACAGAG 
CGATCCAAAT CATATGATGA GGGCCTGGAT GATTACAGAG AAGATGCAAA ACTGTCTTTT 
AAACATGTAT CTAGCCTGAA GGGAATCAAG ATCTTAGACA GTCAAAAGTC ATCAGAAGAT 
TCCGGATCCA GGAAAGACTC TTCCTCAGAG GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG 
CTTCATTTCA GGCCACTCGT CACTGATAAG GGCAAGCGCG TTGGTGGAAG TATTCGGCCA 
TGGAAGCAGA TGTATGTTGT ACTTCGGGGC CATTCGCTGT ATCTGTACAA AGATAAACGA 
GAACAGACGA CTCCGTCTGA GGAGGAGCAG CCCATCAGTG TTAACGCCTG CTTAATAGAC 
ATCTCTTACA GCGAGACCAA GAGGAAGAAT GTCTTTCGAC TCACCACGTC TGACTGCGAG 
TGCCTGTTTC AGGCTGAAGA CAGGGATGAC ATGTTAGCCT GGATCAAAAC CATCCAGGAG 
AGCAGCAATT TAAACGAGGA GGACACTGGA GTCACTAACA GGGACCTAAT TAGTCGAAGA 
ATAAAAGAAT ACAACAGTCT AATGAGCAGC AAAGCAGAGC CATTGCCGAA GACCCCTCGC 
CAGAGTCTCA GCATCCGGCA GACCCTACTT GGTGCTAAAG CAGAGCCTCG GACTCAGAGT 
CCACACTCTC CCAAAGAGGA GTCAGAAAGG AAGCTTCTTA GTAAAGATGA TACCAGTCCT 
CCAAAAGACA AAGGCACGTG GAGAAAAGGC ATTCCAAGTA TTATGAGAAA GACATTTGAG 
AAAAAGCCTA CTGCTACGGG AACATTCGGG GTCAGACTGG ACGACTGCCC ACCAGCTCAT 
ACAAACCGGT ATATTCCATT AATAGTTGAC ATATGTTGTA AATTAGTTGA AGAAAGAGGT 
CTGGAATATA CAGGTATTTA CAGAGTCCCT GGAAACAATG CAGCCATCTC CAGTATGCAG 
GAGGAGCTCA ACAAGGGAAT GGCTGATATT GATATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATCA GCAGTTTATT AAAATCCTTC TTCAGAAAAC TCCCAGAACC TCTCTTCACG 
AATGATAAAT ACGCCGACTT TATTGAAGCC AATCGTAAGG AAGATCCTCT AGATCGTCTG 
AAAACATTAA AAAGACTAAT TCATGATTTG CCCGAACATC ATTATGAAAC ACTCAAGTTT 
CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA AATTCAGAAA AAAATAAGAT GGAACCAAGA 
AACCTAGCCA TAGTGTTTGG TCCAACTCTT GTGAGAACCT CCGAAGATAA CATGACACAC 
ATGGTCACTC ACATGCCAGA TCAGTACAAG ATCGTAGAGA CACTCATCCA GCACCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA CCTCTTACAA CAGTGCAGGA GGAAAACACA 
GTAGACTCCC AGCCAGTGCC AAACATAGAT CATTTACTCA CCAACATTGG AAGGACAGGC 
GTCTCCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CAACAAAATC TAAGGGTTCT 
TGGGGATCCG GAAAAGACCA GTACAGCAAG GACCTGCTTG TATCCTCCAT CTTCGCGGCT 
GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA AAAGCACAAC CCAGCAGCTC GGAAGACGAG 
CTGGACAATG TGTTTTTCAA GAAAGAAAAC TCAGAGCAGG GTCACCACGA CATTAAAGAA 
GAGTCCAAAA AAGAGAGTGA GACACCAGGC CGGAAACAAA GGACCGTTGC TCCCAAAGAA 
AACAACACGA AGAAAGACAG CAGCGGAGGT GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG 
GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC ACCAAGCAGA GCCGGTCACC CACCCTGAGC 
TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC AGGGCCCTCG CAGCCCAGAA GACCTCCCAC 
CTGGAAGACA CGGGGTCCGA CTCCAGCACC CCACTCAGCA CTCACGCCCC GGCTCCCCCG 
GCAAGCTTTC CCACCAAGAA ACCTCCCAGC CCCGAGCCCA AGCACAGCGA GCTCCTGGTC 
AGTGTCGGCA GCATCACCTC CGACCACGCC GGCACACCGT CTGCTCCCTA CCTGGCCGGC 
CTCGACTCCA GCCGGCTGAG CCCCGAGGTG CAGTCCATGG CCGAGAGCCG GGGATTTGAA 
GCTGATGACG AGAGGAGCGA GCTGGTCAGC GAGGGCCGGC CAGTGGAGAC AGACAGCGAG 
AGCGACTTTC CGGTCTTCCC CGCCGCCCCG GCCTCGGACA GGCTCTTCCG AGGAAAACTC 
CAAGAGGCCG CGAGGACCAG CCGGAGAAAC TCAGAAGGCA GTGAGGTCAG CTGCACGGAA 
GGAAGTTTAA CACCAAGTTT AGAAAGCCGG AGACAGCTCT TCAGCTCCCA TAAACTGATC 
GAGTGTGACA CTCTGTCCAG GAAGAAATCC GCCAGGTTCA AGTCAGACAG TGGGAGTCTA 
GGAGATGCCA AGAATGAGAA AGAAGCCCCT TCCATCACCA AAGTGTTCGA CGTTATGAAA 
AAAGGAAAAT CCACCGGAAA TTTACTGACA CCACCAGCCA GAAGCGAATC GGACAAACAG 
GAACCCACTT GGAAAACAAA AATAGCCGAT CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC 
GATGACATGT TTGGAGTAGG AAGTCAAAAA ACCATCGCCG AACCTGCCAA AAGGAAAAAC 
ATCAAACGCA GACACACGCT GGGTGGGCAC AGGGATGCTA CTGAAATGAG TGTTCTGAAT 
TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC AGAGAATCCG AACTTTCAGC TGTGAATCGA 
TTGAAGCCCA AATGCTCCGC CCAGGACCTG TCCATCTCAG ACTGGCTGGC CAGGGAGCGG 
CTACGCACCA GTATGACTGA CCTGAGCAGA GGGGACACGG GGGACCCCCG GCCTGAGAGT 
CTTGGCACCT TAGAAATACC AACCAGGGAC CCGCCCCTGT CTTTCCAGTC TGATGCAGAC 
AGTTCTTCCA GCACCTTGGC TTCAACTAAC AGGGCCCCTC TTTCCACACC ATCACAGTCA 
CCTGACCAAA TAAATGGAGA AAGCTTCCAG AACACGAGCC AAAACTCCAG TTCTGCAGCC 
AGGGTCCAAC CTCATCAACT GTCTGAAACC CCAGATCACA AAGCACAGTT CCATCCCTGT 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_025000959.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856727.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.

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CloneID	OBa221857
Clone ID Related Accession (Same CDS sequence)	XM_025000959.1
Accession Version	XM_025000959.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGGA GCCTCGACAC ATGTAGAGAG AGAGCGTCTG AAGGGTCCTC TCAGTGGAGG 
CATCTGGCTG CTGTGCCCTT CGCTGTGTGC CTGCAGTTTG CCCTTGGTGT AAAATCCGGC 
AGATCAAAGG CTCTGCGGGT ATCAAAAAGT AAAGATGGAA AAGAACAAAG TGAAACTGTA 
TCACCATCCG AAGATGAAAT GTTCTCCTGG CCAGGTCCCA AAACAGTTAT GTTGAAAAGA 
ACATCTCAAG GCTTTGGTTT TACATTGAGG CATTTTATTG TTTATCCCCC AGAGTCTGCA 
ATTCAGTTTT CATTTAAGGA TGAAGAAAAT GGCAACAGAG GAGGAAAACA AAGAAACCGT 
TTGGAACCCA TGGACACCAT CTTTGTTAAA CAGGTGAAAG AGGGCGGACC TGCTTTTGAA 
GCTGGATTAT GCACAGGTGA TCGAATTATA AAAGTCAATG GAGAAAGTGT TATTGGAAAG 
ACCTATTCTC AAGTAATTGC TTTAATCCAG AACAGTGATA CAACATTGGA ACTTAGTGTT 
ATGCCAAAAG ATGAAGACAT TCTCCAAGTG CTACAATTTA CAAAGGATGT CACAGCTCTG 
GCGTATTCTC AAGATGCCTA CCTGAAAGGC AATGAAGCCT ACAGCGGCAA TGCCCGCAAC 
ATCCCTGAGC CCCCACCAGT CTGCTACCCC TGGCCGTCAT CCGCCCCTCC CGCCATGGCG 
CAGCCCCCTG AACCACCTCC TCCAGACTCC ACAGCAAGCA AACCGCAGAC TAGCCCCCCA 
GTATTGACGC AGCCCGGCAG GGCATACAGG ATGGAAATCC AAGTGCCTCC GTCACCACCA 
GAGGTGGCCA AGTCCAACAC AGCAGTGTGT GTCTGCAATG AGAGTGTGAG GACCGTCATT 
GTACCTTCTG AGAAGGTTGT AGATTTATTA CCTAACAGAA GCAACCACAC AGTTCCCGCT 
CACAGAACTG AGGAAGTGAG GTATGGCCGA AACGAACAGC CCTCTTTCAA AACAGCGACG 
AGAACCACCT CACCGCCATC CTCAGTTCCC ACTGCCCATC TCATCCACCA GCCCGGCTCC 
AGGTCGTTGG AACCTTCTGG CATTTTACTT AAATCTGGCC ACTACGGCGG ACACTCAGAA 
GGTCTGGTGA GCAGATCTCC AGCTGTGGAG TCTCCTTCCG TAACTGTCAA CCACTACTCT 
CCCAACTCCC ATCAGCACAT AGACTGGAAA AACTATAAAA CTTACAAAGA GTATATTGAT 
AACCGGCGCT TGCACATGGG CTGTCGGACC ATTCAAGAAA GGTTAGATAG TTTAAGAGCC 
GCCTCTCAGA GCACAGCGGA TTATAACCAG GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA 
CGGCGTCGGA GCACCTCTCA GGACCGAGTG CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC 
GTGTCCCAGG AGAGGCTGGA GGACTCTGTG TTGATGATGT ATTGTCCGAG GAGTGCCTCT 
CAAGGTGCGC TGACATCCCC TTCTATTAGT TTTAGTAACC ACAGAACTCG CTCGTGGGAT 
TACATCGAGG GACAGGGTGA AACCTCGGAA AACGTCCATT CTGAATGTCC ACCGCCGGAT 
GCAAATGGTG AACGAAAGCA GACTTACAAG TGGAGTGGGT TCACCGAACA GGACGATCGT 
CGAGGTATTT ACGAGAGACC CAGGCAGCAA GAAATTCACA AATCTTTCCG AGGCTCCAAT 
TTTACAGTGG CTCCCAGTGT GGTGAATTCT GACAGCAGGA GAGTGAGCAG CAGAATCGGG 
GGATCAGTGT CTCAGTTTAA AAAAATTCCA GCAGATCTAA AACCCCTGCA GCCCAGCAGA 
AATTTTCAAA CTGCTTGTGG AATATCCCAG CCTCGAGGGA TTTCCCAAGA CAGGTCACCT 
CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA GGGCCTTCTA TGCATGTCGC GAAACCGCCC 
TCCAGCCAGA ACTCATTTCT TTCTATCAAA GACCAAAGAC CAGTAAATCA CTTGCATCAA 
AACAGTTTAT TGAATCAGCA GCCATGGTTA CGGACGGAAA GTGCCCCCGA TCACCAAGTG 
GACACTGGGA AGCCCCCTTC TCTTTCCGGA GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT 
GAGAATGCTA GCACTCCAGA TTTTGAGTTA TCCGTCTCTC AGAGGAATCA AGATTTAAAT 
GTACAAGAGG TGGAAATTCA GCAATCGAAT GCTGTAGATA ATAAAGAAAC TGTCATCCTA 
AGAGAAAAAC CTCCGTCTGG TCGCCAGACA CCGCAGCCTT TAAGGCATCA GTCTTACATC 
TTAGCAGTGA GTGACCAGGA GACAGGCTCA GACACCACCT GCTGGCTGCC TAATGATGCT 
CGCCGGGAGG TCCATATAAA AAGGATGGAG GAGAGGAAGG CCTCAAGTAC CACTCCACCT 
GGTGATTCCT TGGCTTCCAT CCCATTTATA GATGAACCTA CCAGCCCTAG CATCGATCAC 
GACATTTCTC ACATCCCCGC CTCTGCGGTC ATCTCTGCCT CCACCTCCCA GGTACCCTCC 
ATAGCAACGG TCCCTCCCAG CCTCACAACT TCAGCTCCAT TAATCCGACG TCAGCTCTCC 
CATGACCAGG AATCTGTTGG ACCTCCCAGC CTGGATGCCC AGCCCAGCTC AAAGACAGAG 
CGATCCAAAT CATATGATGA GGGCCTGGAT GATTACAGAG AAGATGCAAA ACTGTCTTTT 
AAACATGTAT CTAGCCTGAA GGGAATCAAG ATCTTAGACA GTCAAAAGTC ATCAGAAGAT 
TCCGGATCCA GGAAAGACTC TTCCTCAGAG GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG 
CTTCATTTCA GGCCACTCGT CACTGATAAG GGCAAGCGCG TTGGTGGAAG TATTCGGCCA 
TGGAAGCAGA TGTATGTTGT ACTTCGGGGC CATTCGCTGT ATCTGTACAA AGATAAACGA 
GAACAGACGA CTCCGTCTGA GGAGGAGCAG CCCATCAGTG TTAACGCCTG CTTAATAGAC 
ATCTCTTACA GCGAGACCAA GAGGAAGAAT GTCTTTCGAC TCACCACGTC TGACTGCGAG 
TGCCTGTTTC AGGCTGAAGA CAGGGATGAC ATGTTAGCCT GGATCAAAAC CATCCAGGAG 
AGCAGCAATT TAAACGAGGA GGACACTGGA GTCACTAACA GGGACCTAAT TAGTCGAAGA 
ATAAAAGAAT ACAACAGTCT AATGAGCAAA GCAGAGCCAT TGCCGAAGAC CCCTCGCCAG 
AGTCTCAGCA TCCGGCAGAC CCTACTTGGT GCTAAAGCAG AGCCTCGGAC TCAGAGTCCA 
CACTCTCCCA AAGAGGAGTC AGAAAGGAAG CTTCTTAGTA AAGATGATAC CAGTCCTCCA 
AAAGACAAAG GCACGTGGAG AAAAGGCATT CCAAGTATTA TGAGAAAGAC ATTTGAGAAA 
AAGCCTACTG CTACGGGAAC ATTCGGGGTC AGACTGGACG ACTGCCCACC AGCTCATACA 
AACCGGTATA TTCCATTAAT AGTTGACATA TGTTGTAAAT TAGTTGAAGA AAGAGGTCTG 
GAATATACAG GTATTTACAG AGTCCCTGGA AACAATGCAG CCATCTCCAG TATGCAGGAG 
GAGCTCAACA AGGGAATGGC TGATATTGAT ATACAAGATG ATAAATGGCG AGATTTGAAT 
GTGATCAGCA GTTTATTAAA ATCCTTCTTC AGAAAACTCC CAGAACCTCT CTTCACGAAT 
GATAAATACG CCGACTTTAT TGAAGCCAAT CGTAAGGAAG ATCCTCTAGA TCGTCTGAAA 
ACATTAAAAA GACTAATTCA TGATTTGCCC GAACATCATT ATGAAACACT CAAGTTTCTT 
TCGGCTCATC TGAAGACAGT AGCAGAAAAT TCAGAAAAAA ATAAGATGGA ACCAAGAAAC 
CTAGCCATAG TGTTTGGTCC AACTCTTGTG AGAACCTCCG AAGATAACAT GACACACATG 
GTCACTCACA TGCCAGATCA GTACAAGATC GTAGAGACAC TCATCCAGCA CCATGACTGG 
TTTTTCACAG AAGAAGGTGC TGAAGAACCT CTTACAACAG TGCAGGAGGA AAACACAGTA 
GACTCCCAGC CAGTGCCAAA CATAGATCAT TTACTCACCA ACATTGGAAG GACAGGCGTC 
TCCCCTGGAG ATGTATCAGA TTCAGCTACT AGTGACTCAA CAAAATCTAA GGGTTCTTGG 
GGATCCGGAA AAGACCAGTA CAGCAAGGAC CTGCTTGTAT CCTCCATCTT CGCGGCTGCC 
AGTCGCAAGA GGAAAAAGCC AAAAGAAAAA GCACAACCCA GCAGCTCGGA AGACGAGCTG 
GACAATGTGT TTTTCAAGAA AGAAAACTCA GAGCAGGGTC ACCACGACAT TAAAGAAGAG 
TCCAAAAAAG AGAGTGAGAC ACCAGGCCGG AAACAAAGGA CCGTTGCTCC CAAAGAAAAC 
AACACGAAGA AAGACAGCAG CGGAGGTGGC AGAGCTGAGC AGAGGGCCCC GCGTGGGGAG 
AGCTTGGAGC CCCCGGCCCC CCAAAGCACC AAGCAGAGCC GGTCACCCAC CCTGAGCTGT 
CGCCTCGCCG TCCTGAGAGA GAGCCCCAGG GCCCTCGCAG CCCAGAAGAC CTCCCACCTG 
GAAGACACGG GGTCCGACTC CAGCACCCCA CTCAGCACTC ACGCCCCGGC TCCCCCGGCA 
AGCTTTCCCA CCAAGAAACC TCCCAGCCCC GAGCCCAAGC ACAGCGAGCT CCTGGTCAGT 
GTCGGCAGCA TCACCTCCGA CCACGCCGGC ACACCGTCTG CTCCCTACCT GGCCGGCCTC 
GACTCCAGCC GGCTGAGCCC CGAGGTGCAG TCCATGGCCG AGAGCCGGGG ATTTGAAGCT 
GATGACGAGA GGAGCGAGCT GGTCAGCGAG GGCCGGCCAG TGGAGACAGA CAGCGAGAGC 
GACTTTCCGG TCTTCCCCGC CGCCCCGGCC TCGGACAGGC TCTTCCGAGG AAAACTCCAA 
GAGGCCGCGA GGACCAGCCG GAGAAACTCA GAAGGCAGTG AGGTCAGCTG CACGGAAGGA 
AGTTTAACAC CAAGTTTAGA AAGCCGGAGA CAGCTCTTCA GCTCCCATAA ACTGATCGAG 
TGTGACACTC TGTCCAGGAA GAAATCCGCC AGGTTCAAGT CAGACAGTGG GAGTCTAGGA 
GATGCCAAGA ATGAGAAAGA AGCCCCTTCC ATCACCAAAG TGTTCGACGT TATGAAAAAA 
GGAAAATCCA CCGGAAATTT ACTGACACCA CCAGCCAGAA GCGAATCGGA CAAACAGGAA 
CCCACTTGGA AAACAAAAAT AGCCGATCGG TTAAAACTGA GGCCCAAAGC CCCGGCCGAT 
GACATGTTTG GAGTAGGAAG TCAAAAAACC ATCGCCGAAC CTGCCAAAAG GAAAAACATC 
AAACGCAGAC ACACGCTGGG TGGGCACAGG GATGCTACTG AAATGAGTGT TCTGAATTTC 
TGGAAAGCCC AGGAGCACAG CGGGGACAGA GAATCCGAAC TTTCAGCTGT GAATCGATTG 
AAGCCCAAAT GCTCCGCCCA GGACCTGTCC ATCTCAGACT GGCTGGCCAG GGAGCGGCTA 
CGCACCAGTA TGACTGACCT GAGCAGAGGG GACACGGGGG ACCCCCGGCC TGAGAGTCTT 
GGCACCTTAG AAATACCAAC CAGGGACCCG CCCCTGTCTT TCCAGTCTGA TGCAGACAGT 
TCTTCCAGCA CCTTGGCTTC AACTAACAGG GCCCCTCTTT CCACACCATC ACAGTCACCT 
GACCAAATAA ATGGAGAAAG CTTCCAGAAC ACGAGCCAAA ACTCCAGTTC TGCAGCCAGG 
GTCCAACCTC ATCAACTGTC TGAAACCCCA GATCACAAAG CACAGTTCCA TCCCTGTCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856727.1
CDS	74..6016
Translation	MVGSLDTCRERASEGSSQWRHLAAVPFAVCLQFALGVKSGRSKALRVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_025000959.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000959.1

ORF Insert Sequence:

ATGGTGGGGA GCCTCGACAC ATGTAGAGAG AGAGCGTCTG AAGGGTCCTC TCAGTGGAGG 
CATCTGGCTG CTGTGCCCTT CGCTGTGTGC CTGCAGTTTG CCCTTGGTGT AAAATCCGGC 
AGATCAAAGG CTCTGCGGGT ATCAAAAAGT AAAGATGGAA AAGAACAAAG TGAAACTGTA 
TCACCATCCG AAGATGAAAT GTTCTCCTGG CCAGGTCCCA AAACAGTTAT GTTGAAAAGA 
ACATCTCAAG GCTTTGGTTT TACATTGAGG CATTTTATTG TTTATCCCCC AGAGTCTGCA 
ATTCAGTTTT CATTTAAGGA TGAAGAAAAT GGCAACAGAG GAGGAAAACA AAGAAACCGT 
TTGGAACCCA TGGACACCAT CTTTGTTAAA CAGGTGAAAG AGGGCGGACC TGCTTTTGAA 
GCTGGATTAT GCACAGGTGA TCGAATTATA AAAGTCAATG GAGAAAGTGT TATTGGAAAG 
ACCTATTCTC AAGTAATTGC TTTAATCCAG AACAGTGATA CAACATTGGA ACTTAGTGTT 
ATGCCAAAAG ATGAAGACAT TCTCCAAGTG CTACAATTTA CAAAGGATGT CACAGCTCTG 
GCGTATTCTC AAGATGCCTA CCTGAAAGGC AATGAAGCCT ACAGCGGCAA TGCCCGCAAC 
ATCCCTGAGC CCCCACCAGT CTGCTACCCC TGGCCGTCAT CCGCCCCTCC CGCCATGGCG 
CAGCCCCCTG AACCACCTCC TCCAGACTCC ACAGCAAGCA AACCGCAGAC TAGCCCCCCA 
GTATTGACGC AGCCCGGCAG GGCATACAGG ATGGAAATCC AAGTGCCTCC GTCACCACCA 
GAGGTGGCCA AGTCCAACAC AGCAGTGTGT GTCTGCAATG AGAGTGTGAG GACCGTCATT 
GTACCTTCTG AGAAGGTTGT AGATTTATTA CCTAACAGAA GCAACCACAC AGTTCCCGCT 
CACAGAACTG AGGAAGTGAG GTATGGCCGA AACGAACAGC CCTCTTTCAA AACAGCGACG 
AGAACCACCT CACCGCCATC CTCAGTTCCC ACTGCCCATC TCATCCACCA GCCCGGCTCC 
AGGTCGTTGG AACCTTCTGG CATTTTACTT AAATCTGGCC ACTACGGCGG ACACTCAGAA 
GGTCTGGTGA GCAGATCTCC AGCTGTGGAG TCTCCTTCCG TAACTGTCAA CCACTACTCT 
CCCAACTCCC ATCAGCACAT AGACTGGAAA AACTATAAAA CTTACAAAGA GTATATTGAT 
AACCGGCGCT TGCACATGGG CTGTCGGACC ATTCAAGAAA GGTTAGATAG TTTAAGAGCC 
GCCTCTCAGA GCACAGCGGA TTATAACCAG GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA 
CGGCGTCGGA GCACCTCTCA GGACCGAGTG CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC 
GTGTCCCAGG AGAGGCTGGA GGACTCTGTG TTGATGATGT ATTGTCCGAG GAGTGCCTCT 
CAAGGTGCGC TGACATCCCC TTCTATTAGT TTTAGTAACC ACAGAACTCG CTCGTGGGAT 
TACATCGAGG GACAGGGTGA AACCTCGGAA AACGTCCATT CTGAATGTCC ACCGCCGGAT 
GCAAATGGTG AACGAAAGCA GACTTACAAG TGGAGTGGGT TCACCGAACA GGACGATCGT 
CGAGGTATTT ACGAGAGACC CAGGCAGCAA GAAATTCACA AATCTTTCCG AGGCTCCAAT 
TTTACAGTGG CTCCCAGTGT GGTGAATTCT GACAGCAGGA GAGTGAGCAG CAGAATCGGG 
GGATCAGTGT CTCAGTTTAA AAAAATTCCA GCAGATCTAA AACCCCTGCA GCCCAGCAGA 
AATTTTCAAA CTGCTTGTGG AATATCCCAG CCTCGAGGGA TTTCCCAAGA CAGGTCACCT 
CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA GGGCCTTCTA TGCATGTCGC GAAACCGCCC 
TCCAGCCAGA ACTCATTTCT TTCTATCAAA GACCAAAGAC CAGTAAATCA CTTGCATCAA 
AACAGTTTAT TGAATCAGCA GCCATGGTTA CGGACGGAAA GTGCCCCCGA TCACCAAGTG 
GACACTGGGA AGCCCCCTTC TCTTTCCGGA GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT 
GAGAATGCTA GCACTCCAGA TTTTGAGTTA TCCGTCTCTC AGAGGAATCA AGATTTAAAT 
GTACAAGAGG TGGAAATTCA GCAATCGAAT GCTGTAGATA ATAAAGAAAC TGTCATCCTA 
AGAGAAAAAC CTCCGTCTGG TCGCCAGACA CCGCAGCCTT TAAGGCATCA GTCTTACATC 
TTAGCAGTGA GTGACCAGGA GACAGGCTCA GACACCACCT GCTGGCTGCC TAATGATGCT 
CGCCGGGAGG TCCATATAAA AAGGATGGAG GAGAGGAAGG CCTCAAGTAC CACTCCACCT 
GGTGATTCCT TGGCTTCCAT CCCATTTATA GATGAACCTA CCAGCCCTAG CATCGATCAC 
GACATTTCTC ACATCCCCGC CTCTGCGGTC ATCTCTGCCT CCACCTCCCA GGTACCCTCC 
ATAGCAACGG TCCCTCCCAG CCTCACAACT TCAGCTCCAT TAATCCGACG TCAGCTCTCC 
CATGACCAGG AATCTGTTGG ACCTCCCAGC CTGGATGCCC AGCCCAGCTC AAAGACAGAG 
CGATCCAAAT CATATGATGA GGGCCTGGAT GATTACAGAG AAGATGCAAA ACTGTCTTTT 
AAACATGTAT CTAGCCTGAA GGGAATCAAG ATCTTAGACA GTCAAAAGTC ATCAGAAGAT 
TCCGGATCCA GGAAAGACTC TTCCTCAGAG GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG 
CTTCATTTCA GGCCACTCGT CACTGATAAG GGCAAGCGCG TTGGTGGAAG TATTCGGCCA 
TGGAAGCAGA TGTATGTTGT ACTTCGGGGC CATTCGCTGT ATCTGTACAA AGATAAACGA 
GAACAGACGA CTCCGTCTGA GGAGGAGCAG CCCATCAGTG TTAACGCCTG CTTAATAGAC 
ATCTCTTACA GCGAGACCAA GAGGAAGAAT GTCTTTCGAC TCACCACGTC TGACTGCGAG 
TGCCTGTTTC AGGCTGAAGA CAGGGATGAC ATGTTAGCCT GGATCAAAAC CATCCAGGAG 
AGCAGCAATT TAAACGAGGA GGACACTGGA GTCACTAACA GGGACCTAAT TAGTCGAAGA 
ATAAAAGAAT ACAACAGTCT AATGAGCAAA GCAGAGCCAT TGCCGAAGAC CCCTCGCCAG 
AGTCTCAGCA TCCGGCAGAC CCTACTTGGT GCTAAAGCAG AGCCTCGGAC TCAGAGTCCA 
CACTCTCCCA AAGAGGAGTC AGAAAGGAAG CTTCTTAGTA AAGATGATAC CAGTCCTCCA 
AAAGACAAAG GCACGTGGAG AAAAGGCATT CCAAGTATTA TGAGAAAGAC ATTTGAGAAA 
AAGCCTACTG CTACGGGAAC ATTCGGGGTC AGACTGGACG ACTGCCCACC AGCTCATACA 
AACCGGTATA TTCCATTAAT AGTTGACATA TGTTGTAAAT TAGTTGAAGA AAGAGGTCTG 
GAATATACAG GTATTTACAG AGTCCCTGGA AACAATGCAG CCATCTCCAG TATGCAGGAG 
GAGCTCAACA AGGGAATGGC TGATATTGAT ATACAAGATG ATAAATGGCG AGATTTGAAT 
GTGATCAGCA GTTTATTAAA ATCCTTCTTC AGAAAACTCC CAGAACCTCT CTTCACGAAT 
GATAAATACG CCGACTTTAT TGAAGCCAAT CGTAAGGAAG ATCCTCTAGA TCGTCTGAAA 
ACATTAAAAA GACTAATTCA TGATTTGCCC GAACATCATT ATGAAACACT CAAGTTTCTT 
TCGGCTCATC TGAAGACAGT AGCAGAAAAT TCAGAAAAAA ATAAGATGGA ACCAAGAAAC 
CTAGCCATAG TGTTTGGTCC AACTCTTGTG AGAACCTCCG AAGATAACAT GACACACATG 
GTCACTCACA TGCCAGATCA GTACAAGATC GTAGAGACAC TCATCCAGCA CCATGACTGG 
TTTTTCACAG AAGAAGGTGC TGAAGAACCT CTTACAACAG TGCAGGAGGA AAACACAGTA 
GACTCCCAGC CAGTGCCAAA CATAGATCAT TTACTCACCA ACATTGGAAG GACAGGCGTC 
TCCCCTGGAG ATGTATCAGA TTCAGCTACT AGTGACTCAA CAAAATCTAA GGGTTCTTGG 
GGATCCGGAA AAGACCAGTA CAGCAAGGAC CTGCTTGTAT CCTCCATCTT CGCGGCTGCC 
AGTCGCAAGA GGAAAAAGCC AAAAGAAAAA GCACAACCCA GCAGCTCGGA AGACGAGCTG 
GACAATGTGT TTTTCAAGAA AGAAAACTCA GAGCAGGGTC ACCACGACAT TAAAGAAGAG 
TCCAAAAAAG AGAGTGAGAC ACCAGGCCGG AAACAAAGGA CCGTTGCTCC CAAAGAAAAC 
AACACGAAGA AAGACAGCAG CGGAGGTGGC AGAGCTGAGC AGAGGGCCCC GCGTGGGGAG 
AGCTTGGAGC CCCCGGCCCC CCAAAGCACC AAGCAGAGCC GGTCACCCAC CCTGAGCTGT 
CGCCTCGCCG TCCTGAGAGA GAGCCCCAGG GCCCTCGCAG CCCAGAAGAC CTCCCACCTG 
GAAGACACGG GGTCCGACTC CAGCACCCCA CTCAGCACTC ACGCCCCGGC TCCCCCGGCA 
AGCTTTCCCA CCAAGAAACC TCCCAGCCCC GAGCCCAAGC ACAGCGAGCT CCTGGTCAGT 
GTCGGCAGCA TCACCTCCGA CCACGCCGGC ACACCGTCTG CTCCCTACCT GGCCGGCCTC 
GACTCCAGCC GGCTGAGCCC CGAGGTGCAG TCCATGGCCG AGAGCCGGGG ATTTGAAGCT 
GATGACGAGA GGAGCGAGCT GGTCAGCGAG GGCCGGCCAG TGGAGACAGA CAGCGAGAGC 
GACTTTCCGG TCTTCCCCGC CGCCCCGGCC TCGGACAGGC TCTTCCGAGG AAAACTCCAA 
GAGGCCGCGA GGACCAGCCG GAGAAACTCA GAAGGCAGTG AGGTCAGCTG CACGGAAGGA 
AGTTTAACAC CAAGTTTAGA AAGCCGGAGA CAGCTCTTCA GCTCCCATAA ACTGATCGAG 
TGTGACACTC TGTCCAGGAA GAAATCCGCC AGGTTCAAGT CAGACAGTGG GAGTCTAGGA 
GATGCCAAGA ATGAGAAAGA AGCCCCTTCC ATCACCAAAG TGTTCGACGT TATGAAAAAA 
GGAAAATCCA CCGGAAATTT ACTGACACCA CCAGCCAGAA GCGAATCGGA CAAACAGGAA 
CCCACTTGGA AAACAAAAAT AGCCGATCGG TTAAAACTGA GGCCCAAAGC CCCGGCCGAT 
GACATGTTTG GAGTAGGAAG TCAAAAAACC ATCGCCGAAC CTGCCAAAAG GAAAAACATC 
AAACGCAGAC ACACGCTGGG TGGGCACAGG GATGCTACTG AAATGAGTGT TCTGAATTTC 
TGGAAAGCCC AGGAGCACAG CGGGGACAGA GAATCCGAAC TTTCAGCTGT GAATCGATTG 
AAGCCCAAAT GCTCCGCCCA GGACCTGTCC ATCTCAGACT GGCTGGCCAG GGAGCGGCTA 
CGCACCAGTA TGACTGACCT GAGCAGAGGG GACACGGGGG ACCCCCGGCC TGAGAGTCTT 
GGCACCTTAG AAATACCAAC CAGGGACCCG CCCCTGTCTT TCCAGTCTGA TGCAGACAGT 
TCTTCCAGCA CCTTGGCTTC AACTAACAGG GCCCCTCTTT CCACACCATC ACAGTCACCT 
GACCAAATAA ATGGAGAAAG CTTCCAGAAC ACGAGCCAAA ACTCCAGTTC TGCAGCCAGG 
GTCCAACCTC ATCAACTGTC TGAAACCCCA GATCACAAAG CACAGTTCCA TCCCTGTCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_025000960.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856728.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X3, mRNA.

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CloneID	OBa221858
Clone ID Related Accession (Same CDS sequence)	XM_025000960.1
Accession Version	XM_025000960.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGGA GCCTCGACAC ATGTAGAGAG AGAGCGTCTG AAGGGTCCTC TCAGTGGAGG 
CATCTGGCTG CTGTGCCCTT CGCTGTGTGC CTGCAGTTTG CCCTTGGTGT AAAATCCGGC 
AGATCAAAGG CTCTGCGGGT ATCAAAAAGT AAAGATGGAA AAGAACAAAG TGAAACTGTA 
TCACCATCCG AAGATGAAAT GTTCTCCTGG CCAGGTCCCA AAACAGTTAT GTTGAAAAGA 
ACATCTCAAG GCTTTGGTTT TACATTGAGG CATTTTATTG TTTATCCCCC AGAGTCTGCA 
ATTCAGTTTT CATTTAAGGA TGAAGAAAAT GGCAACAGAG GAGGAAAACA AAGAAACCGT 
TTGGAACCCA TGGACACCAT CTTTGTTAAA CAGGTGAAAG AGGGCGGACC TGCTTTTGAA 
GCTGGATTAT GCACAGGTGA TCGAATTATA AAAGTCAATG GAGAAAGTGT TATTGGAAAG 
ACCTATTCTC AAGTAATTGC TTTAATCCAG AACAGTGATA CAACATTGGA ACTTAGTGTT 
ATGCCAAAAG ATGAAGACAT TCTCCAAGTG GCGTATTCTC AAGATGCCTA CCTGAAAGGC 
AATGAAGCCT ACAGCGGCAA TGCCCGCAAC ATCCCTGAGC CCCCACCAGT CTGCTACCCC 
TGGCCGTCAT CCGCCCCTCC CGCCATGGCG CAGCCCCCTG AACCACCTCC TCCAGACTCC 
ACAGCAAGCA AACCGCAGAC TAGCCCCCCA GTATTGACGC AGCCCGGCAG GGCATACAGG 
ATGGAAATCC AAGTGCCTCC GTCACCACCA GAGGTGGCCA AGTCCAACAC AGCAGTGTGT 
GTCTGCAATG AGAGTGTGAG GACCGTCATT GTACCTTCTG AGAAGGTTGT AGATTTATTA 
CCTAACAGAA GCAACCACAC AGTTCCCGCT CACAGAACTG AGGAAGTGAG GTATGGCCGA 
AACGAACAGC CCTCTTTCAA AACAGCGACG AGAACCACCT CACCGCCATC CTCAGTTCCC 
ACTGCCCATC TCATCCACCA GCCCGGCTCC AGGTCGTTGG AACCTTCTGG CATTTTACTT 
AAATCTGGCC ACTACGGCGG ACACTCAGAA GGTCTGGTGA GCAGATCTCC AGCTGTGGAG 
TCTCCTTCCG TAACTGTCAA CCACTACTCT CCCAACTCCC ATCAGCACAT AGACTGGAAA 
AACTATAAAA CTTACAAAGA GTATATTGAT AACCGGCGCT TGCACATGGG CTGTCGGACC 
ATTCAAGAAA GGTTAGATAG TTTAAGAGCC GCCTCTCAGA GCACAGCGGA TTATAACCAG 
GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA CGGCGTCGGA GCACCTCTCA GGACCGAGTG 
CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC GTGTCCCAGG AGAGGCTGGA GGACTCTGTG 
TTGATGATGT ATTGTCCGAG GAGTGCCTCT CAAGGTGCGC TGACATCCCC TTCTATTAGT 
TTTAGTAACC ACAGAACTCG CTCGTGGGAT TACATCGAGG GACAGGGTGA AACCTCGGAA 
AACGTCCATT CTGAATGTCC ACCGCCGGAT GCAAATGGTG AACGAAAGCA GACTTACAAG 
TGGAGTGGGT TCACCGAACA GGACGATCGT CGAGGTATTT ACGAGAGACC CAGGCAGCAA 
GAAATTCACA AATCTTTCCG AGGCTCCAAT TTTACAGTGG CTCCCAGTGT GGTGAATTCT 
GACAGCAGGA GAGTGAGCAG CAGAATCGGG GGATCAGTGT CTCAGTTTAA AAAAATTCCA 
GCAGATCTAA AACCCCTGCA GCCCAGCAGA AATTTTCAAA CTGCTTGTGG AATATCCCAG 
CCTCGAGGGA TTTCCCAAGA CAGGTCACCT CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA 
GGGCCTTCTA TGCATGTCGC GAAACCGCCC TCCAGCCAGA ACTCATTTCT TTCTATCAAA 
GACCAAAGAC CAGTAAATCA CTTGCATCAA AACAGTTTAT TGAATCAGCA GCCATGGTTA 
CGGACGGAAA GTGCCCCCGA TCACCAAGTG GACACTGGGA AGCCCCCTTC TCTTTCCGGA 
GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT GAGAATGCTA GCACTCCAGA TTTTGAGTTA 
TCCGTCTCTC AGAGGAATCA AGATTTAAAT GTACAAGAGG TGGAAATTCA GCAATCGAAT 
GCTGTAGATA ATAAAGAAAC TGTCATCCTA AGAGAAAAAC CTCCGTCTGG TCGCCAGACA 
CCGCAGCCTT TAAGGCATCA GTCTTACATC TTAGCAGTGA GTGACCAGGA GACAGGCTCA 
GACACCACCT GCTGGCTGCC TAATGATGCT CGCCGGGAGG TCCATATAAA AAGGATGGAG 
GAGAGGAAGG CCTCAAGTAC CACTCCACCT GGTGATTCCT TGGCTTCCAT CCCATTTATA 
GATGAACCTA CCAGCCCTAG CATCGATCAC GACATTTCTC ACATCCCCGC CTCTGCGGTC 
ATCTCTGCCT CCACCTCCCA GGTACCCTCC ATAGCAACGG TCCCTCCCAG CCTCACAACT 
TCAGCTCCAT TAATCCGACG TCAGCTCTCC CATGACCAGG AATCTGTTGG ACCTCCCAGC 
CTGGATGCCC AGCCCAGCTC AAAGACAGAG CGATCCAAAT CATATGATGA GGGCCTGGAT 
GATTACAGAG AAGATGCAAA ACTGTCTTTT AAACATGTAT CTAGCCTGAA GGGAATCAAG 
ATCTTAGACA GTCAAAAGTC ATCAGAAGAT TCCGGATCCA GGAAAGACTC TTCCTCAGAG 
GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG CTTCATTTCA GGCCACTCGT CACTGATAAG 
GGCAAGCGCG TTGGTGGAAG TATTCGGCCA TGGAAGCAGA TGTATGTTGT ACTTCGGGGC 
CATTCGCTGT ATCTGTACAA AGATAAACGA GAACAGACGA CTCCGTCTGA GGAGGAGCAG 
CCCATCAGTG TTAACGCCTG CTTAATAGAC ATCTCTTACA GCGAGACCAA GAGGAAGAAT 
GTCTTTCGAC TCACCACGTC TGACTGCGAG TGCCTGTTTC AGGCTGAAGA CAGGGATGAC 
ATGTTAGCCT GGATCAAAAC CATCCAGGAG AGCAGCAATT TAAACGAGGA GGACACTGGA 
GTCACTAACA GGGACCTAAT TAGTCGAAGA ATAAAAGAAT ACAACAGTCT AATGAGCAGC 
AAAGCAGAGC CATTGCCGAA GACCCCTCGC CAGAGTCTCA GCATCCGGCA GACCCTACTT 
GGTGCTAAAG CAGAGCCTCG GACTCAGAGT CCACACTCTC CCAAAGAGGA GTCAGAAAGG 
AAGCTTCTTA GTAAAGATGA TACCAGTCCT CCAAAAGACA AAGGCACGTG GAGAAAAGGC 
ATTCCAAGTA TTATGAGAAA GACATTTGAG AAAAAGCCTA CTGCTACGGG AACATTCGGG 
GTCAGACTGG ACGACTGCCC ACCAGCTCAT ACAAACCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGTA AATTAGTTGA AGAAAGAGGT CTGGAATATA CAGGTATTTA CAGAGTCCCT 
GGAAACAATG CAGCCATCTC CAGTATGCAG GAGGAGCTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATCA GCAGTTTATT AAAATCCTTC 
TTCAGAAAAC TCCCAGAACC TCTCTTCACG AATGATAAAT ACGCCGACTT TATTGAAGCC 
AATCGTAAGG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCATGATTTG 
CCCGAACATC ATTATGAAAC ACTCAAGTTT CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCCA TAGTGTTTGG TCCAACTCTT 
GTGAGAACCT CCGAAGATAA CATGACACAC ATGGTCACTC ACATGCCAGA TCAGTACAAG 
ATCGTAGAGA CACTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA 
CCTCTTACAA CAGTGCAGGA GGAAAACACA GTAGACTCCC AGCCAGTGCC AAACATAGAT 
CATTTACTCA CCAACATTGG AAGGACAGGC GTCTCCCCTG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CAACAAAATC TAAGGGTTCT TGGGGATCCG GAAAAGACCA GTACAGCAAG 
GACCTGCTTG TATCCTCCAT CTTCGCGGCT GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA 
AAAGCACAAC CCAGCAGCTC GGAAGACGAG CTGGACAATG TGTTTTTCAA GAAAGAAAAC 
TCAGAGCAGG GTCACCACGA CATTAAAGAA GAGTCCAAAA AAGAGAGTGA GACACCAGGC 
CGGAAACAAA GGACCGTTGC TCCCAAAGAA AACAACACGA AGAAAGACAG CAGCGGAGGT 
GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC 
ACCAAGCAGA GCCGGTCACC CACCCTGAGC TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC 
AGGGCCCTCG CAGCCCAGAA GACCTCCCAC CTGGAAGACA CGGGGTCCGA CTCCAGCACC 
CCACTCAGCA CTCACGCCCC GGCTCCCCCG GCAAGCTTTC CCACCAAGAA ACCTCCCAGC 
CCCGAGCCCA AGCACAGCGA GCTCCTGGTC AGTGTCGGCA GCATCACCTC CGACCACGCC 
GGCACACCGT CTGCTCCCTA CCTGGCCGGC CTCGACTCCA GCCGGCTGAG CCCCGAGGTG 
CAGTCCATGG CCGAGAGCCG GGGATTTGAA GCTGATGACG AGAGGAGCGA GCTGGTCAGC 
GAGGGCCGGC CAGTGGAGAC AGACAGCGAG AGCGACTTTC CGGTCTTCCC CGCCGCCCCG 
GCCTCGGACA GGCTCTTCCG AGGAAAACTC CAAGAGGCCG CGAGGACCAG CCGGAGAAAC 
TCAGAAGGCA GTGAGGTCAG CTGCACGGAA GGAAGTTTAA CACCAAGTTT AGAAAGCCGG 
AGACAGCTCT TCAGCTCCCA TAAACTGATC GAGTGTGACA CTCTGTCCAG GAAGAAATCC 
GCCAGGTTCA AGTCAGACAG TGGGAGTCTA GGAGATGCCA AGAATGAGAA AGAAGCCCCT 
TCCATCACCA AAGTGTTCGA CGTTATGAAA AAAGGAAAAT CCACCGGAAA TTTACTGACA 
CCACCAGCCA GAAGCGAATC GGACAAACAG GAACCCACTT GGAAAACAAA AATAGCCGAT 
CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC GATGACATGT TTGGAGTAGG AAGTCAAAAA 
ACCATCGCCG AACCTGCCAA AAGGAAAAAC ATCAAACGCA GACACACGCT GGGTGGGCAC 
AGGGATGCTA CTGAAATGAG TGTTCTGAAT TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC 
AGAGAATCCG AACTTTCAGC TGTGAATCGA TTGAAGCCCA AATGCTCCGC CCAGGACCTG 
TCCATCTCAG ACTGGCTGGC CAGGGAGCGG CTACGCACCA GTATGACTGA CCTGAGCAGA 
GGGGACACGG GGGACCCCCG GCCTGAGAGT CTTGGCACCT TAGAAATACC AACCAGGGAC 
CCGCCCCTGT CTTTCCAGTC TGATGCAGAC AGTTCTTCCA GCACCTTGGC TTCAACTAAC 
AGGGCCCCTC TTTCCACACC ATCACAGTCA CCTGACCAAA TAAATGGAGA AAGCTTCCAG 
AACACGAGCC AAAACTCCAG TTCTGCAGCC AGGGTCCAAC CTCATCAACT GTCTGAAACC 
CCAGATCACA AAGCACAGTT CCATCCCTGT CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856728.1
CDS	73..5988
Translation	MVGSLDTCRERASEGSSQWRHLAAVPFAVCLQFALGVKSGRSKALRVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_025000960.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000960.1

ORF Insert Sequence:

ATGGTGGGGA GCCTCGACAC ATGTAGAGAG AGAGCGTCTG AAGGGTCCTC TCAGTGGAGG 
CATCTGGCTG CTGTGCCCTT CGCTGTGTGC CTGCAGTTTG CCCTTGGTGT AAAATCCGGC 
AGATCAAAGG CTCTGCGGGT ATCAAAAAGT AAAGATGGAA AAGAACAAAG TGAAACTGTA 
TCACCATCCG AAGATGAAAT GTTCTCCTGG CCAGGTCCCA AAACAGTTAT GTTGAAAAGA 
ACATCTCAAG GCTTTGGTTT TACATTGAGG CATTTTATTG TTTATCCCCC AGAGTCTGCA 
ATTCAGTTTT CATTTAAGGA TGAAGAAAAT GGCAACAGAG GAGGAAAACA AAGAAACCGT 
TTGGAACCCA TGGACACCAT CTTTGTTAAA CAGGTGAAAG AGGGCGGACC TGCTTTTGAA 
GCTGGATTAT GCACAGGTGA TCGAATTATA AAAGTCAATG GAGAAAGTGT TATTGGAAAG 
ACCTATTCTC AAGTAATTGC TTTAATCCAG AACAGTGATA CAACATTGGA ACTTAGTGTT 
ATGCCAAAAG ATGAAGACAT TCTCCAAGTG GCGTATTCTC AAGATGCCTA CCTGAAAGGC 
AATGAAGCCT ACAGCGGCAA TGCCCGCAAC ATCCCTGAGC CCCCACCAGT CTGCTACCCC 
TGGCCGTCAT CCGCCCCTCC CGCCATGGCG CAGCCCCCTG AACCACCTCC TCCAGACTCC 
ACAGCAAGCA AACCGCAGAC TAGCCCCCCA GTATTGACGC AGCCCGGCAG GGCATACAGG 
ATGGAAATCC AAGTGCCTCC GTCACCACCA GAGGTGGCCA AGTCCAACAC AGCAGTGTGT 
GTCTGCAATG AGAGTGTGAG GACCGTCATT GTACCTTCTG AGAAGGTTGT AGATTTATTA 
CCTAACAGAA GCAACCACAC AGTTCCCGCT CACAGAACTG AGGAAGTGAG GTATGGCCGA 
AACGAACAGC CCTCTTTCAA AACAGCGACG AGAACCACCT CACCGCCATC CTCAGTTCCC 
ACTGCCCATC TCATCCACCA GCCCGGCTCC AGGTCGTTGG AACCTTCTGG CATTTTACTT 
AAATCTGGCC ACTACGGCGG ACACTCAGAA GGTCTGGTGA GCAGATCTCC AGCTGTGGAG 
TCTCCTTCCG TAACTGTCAA CCACTACTCT CCCAACTCCC ATCAGCACAT AGACTGGAAA 
AACTATAAAA CTTACAAAGA GTATATTGAT AACCGGCGCT TGCACATGGG CTGTCGGACC 
ATTCAAGAAA GGTTAGATAG TTTAAGAGCC GCCTCTCAGA GCACAGCGGA TTATAACCAG 
GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA CGGCGTCGGA GCACCTCTCA GGACCGAGTG 
CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC GTGTCCCAGG AGAGGCTGGA GGACTCTGTG 
TTGATGATGT ATTGTCCGAG GAGTGCCTCT CAAGGTGCGC TGACATCCCC TTCTATTAGT 
TTTAGTAACC ACAGAACTCG CTCGTGGGAT TACATCGAGG GACAGGGTGA AACCTCGGAA 
AACGTCCATT CTGAATGTCC ACCGCCGGAT GCAAATGGTG AACGAAAGCA GACTTACAAG 
TGGAGTGGGT TCACCGAACA GGACGATCGT CGAGGTATTT ACGAGAGACC CAGGCAGCAA 
GAAATTCACA AATCTTTCCG AGGCTCCAAT TTTACAGTGG CTCCCAGTGT GGTGAATTCT 
GACAGCAGGA GAGTGAGCAG CAGAATCGGG GGATCAGTGT CTCAGTTTAA AAAAATTCCA 
GCAGATCTAA AACCCCTGCA GCCCAGCAGA AATTTTCAAA CTGCTTGTGG AATATCCCAG 
CCTCGAGGGA TTTCCCAAGA CAGGTCACCT CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA 
GGGCCTTCTA TGCATGTCGC GAAACCGCCC TCCAGCCAGA ACTCATTTCT TTCTATCAAA 
GACCAAAGAC CAGTAAATCA CTTGCATCAA AACAGTTTAT TGAATCAGCA GCCATGGTTA 
CGGACGGAAA GTGCCCCCGA TCACCAAGTG GACACTGGGA AGCCCCCTTC TCTTTCCGGA 
GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT GAGAATGCTA GCACTCCAGA TTTTGAGTTA 
TCCGTCTCTC AGAGGAATCA AGATTTAAAT GTACAAGAGG TGGAAATTCA GCAATCGAAT 
GCTGTAGATA ATAAAGAAAC TGTCATCCTA AGAGAAAAAC CTCCGTCTGG TCGCCAGACA 
CCGCAGCCTT TAAGGCATCA GTCTTACATC TTAGCAGTGA GTGACCAGGA GACAGGCTCA 
GACACCACCT GCTGGCTGCC TAATGATGCT CGCCGGGAGG TCCATATAAA AAGGATGGAG 
GAGAGGAAGG CCTCAAGTAC CACTCCACCT GGTGATTCCT TGGCTTCCAT CCCATTTATA 
GATGAACCTA CCAGCCCTAG CATCGATCAC GACATTTCTC ACATCCCCGC CTCTGCGGTC 
ATCTCTGCCT CCACCTCCCA GGTACCCTCC ATAGCAACGG TCCCTCCCAG CCTCACAACT 
TCAGCTCCAT TAATCCGACG TCAGCTCTCC CATGACCAGG AATCTGTTGG ACCTCCCAGC 
CTGGATGCCC AGCCCAGCTC AAAGACAGAG CGATCCAAAT CATATGATGA GGGCCTGGAT 
GATTACAGAG AAGATGCAAA ACTGTCTTTT AAACATGTAT CTAGCCTGAA GGGAATCAAG 
ATCTTAGACA GTCAAAAGTC ATCAGAAGAT TCCGGATCCA GGAAAGACTC TTCCTCAGAG 
GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG CTTCATTTCA GGCCACTCGT CACTGATAAG 
GGCAAGCGCG TTGGTGGAAG TATTCGGCCA TGGAAGCAGA TGTATGTTGT ACTTCGGGGC 
CATTCGCTGT ATCTGTACAA AGATAAACGA GAACAGACGA CTCCGTCTGA GGAGGAGCAG 
CCCATCAGTG TTAACGCCTG CTTAATAGAC ATCTCTTACA GCGAGACCAA GAGGAAGAAT 
GTCTTTCGAC TCACCACGTC TGACTGCGAG TGCCTGTTTC AGGCTGAAGA CAGGGATGAC 
ATGTTAGCCT GGATCAAAAC CATCCAGGAG AGCAGCAATT TAAACGAGGA GGACACTGGA 
GTCACTAACA GGGACCTAAT TAGTCGAAGA ATAAAAGAAT ACAACAGTCT AATGAGCAGC 
AAAGCAGAGC CATTGCCGAA GACCCCTCGC CAGAGTCTCA GCATCCGGCA GACCCTACTT 
GGTGCTAAAG CAGAGCCTCG GACTCAGAGT CCACACTCTC CCAAAGAGGA GTCAGAAAGG 
AAGCTTCTTA GTAAAGATGA TACCAGTCCT CCAAAAGACA AAGGCACGTG GAGAAAAGGC 
ATTCCAAGTA TTATGAGAAA GACATTTGAG AAAAAGCCTA CTGCTACGGG AACATTCGGG 
GTCAGACTGG ACGACTGCCC ACCAGCTCAT ACAAACCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGTA AATTAGTTGA AGAAAGAGGT CTGGAATATA CAGGTATTTA CAGAGTCCCT 
GGAAACAATG CAGCCATCTC CAGTATGCAG GAGGAGCTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATCA GCAGTTTATT AAAATCCTTC 
TTCAGAAAAC TCCCAGAACC TCTCTTCACG AATGATAAAT ACGCCGACTT TATTGAAGCC 
AATCGTAAGG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCATGATTTG 
CCCGAACATC ATTATGAAAC ACTCAAGTTT CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCCA TAGTGTTTGG TCCAACTCTT 
GTGAGAACCT CCGAAGATAA CATGACACAC ATGGTCACTC ACATGCCAGA TCAGTACAAG 
ATCGTAGAGA CACTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA 
CCTCTTACAA CAGTGCAGGA GGAAAACACA GTAGACTCCC AGCCAGTGCC AAACATAGAT 
CATTTACTCA CCAACATTGG AAGGACAGGC GTCTCCCCTG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CAACAAAATC TAAGGGTTCT TGGGGATCCG GAAAAGACCA GTACAGCAAG 
GACCTGCTTG TATCCTCCAT CTTCGCGGCT GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA 
AAAGCACAAC CCAGCAGCTC GGAAGACGAG CTGGACAATG TGTTTTTCAA GAAAGAAAAC 
TCAGAGCAGG GTCACCACGA CATTAAAGAA GAGTCCAAAA AAGAGAGTGA GACACCAGGC 
CGGAAACAAA GGACCGTTGC TCCCAAAGAA AACAACACGA AGAAAGACAG CAGCGGAGGT 
GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC 
ACCAAGCAGA GCCGGTCACC CACCCTGAGC TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC 
AGGGCCCTCG CAGCCCAGAA GACCTCCCAC CTGGAAGACA CGGGGTCCGA CTCCAGCACC 
CCACTCAGCA CTCACGCCCC GGCTCCCCCG GCAAGCTTTC CCACCAAGAA ACCTCCCAGC 
CCCGAGCCCA AGCACAGCGA GCTCCTGGTC AGTGTCGGCA GCATCACCTC CGACCACGCC 
GGCACACCGT CTGCTCCCTA CCTGGCCGGC CTCGACTCCA GCCGGCTGAG CCCCGAGGTG 
CAGTCCATGG CCGAGAGCCG GGGATTTGAA GCTGATGACG AGAGGAGCGA GCTGGTCAGC 
GAGGGCCGGC CAGTGGAGAC AGACAGCGAG AGCGACTTTC CGGTCTTCCC CGCCGCCCCG 
GCCTCGGACA GGCTCTTCCG AGGAAAACTC CAAGAGGCCG CGAGGACCAG CCGGAGAAAC 
TCAGAAGGCA GTGAGGTCAG CTGCACGGAA GGAAGTTTAA CACCAAGTTT AGAAAGCCGG 
AGACAGCTCT TCAGCTCCCA TAAACTGATC GAGTGTGACA CTCTGTCCAG GAAGAAATCC 
GCCAGGTTCA AGTCAGACAG TGGGAGTCTA GGAGATGCCA AGAATGAGAA AGAAGCCCCT 
TCCATCACCA AAGTGTTCGA CGTTATGAAA AAAGGAAAAT CCACCGGAAA TTTACTGACA 
CCACCAGCCA GAAGCGAATC GGACAAACAG GAACCCACTT GGAAAACAAA AATAGCCGAT 
CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC GATGACATGT TTGGAGTAGG AAGTCAAAAA 
ACCATCGCCG AACCTGCCAA AAGGAAAAAC ATCAAACGCA GACACACGCT GGGTGGGCAC 
AGGGATGCTA CTGAAATGAG TGTTCTGAAT TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC 
AGAGAATCCG AACTTTCAGC TGTGAATCGA TTGAAGCCCA AATGCTCCGC CCAGGACCTG 
TCCATCTCAG ACTGGCTGGC CAGGGAGCGG CTACGCACCA GTATGACTGA CCTGAGCAGA 
GGGGACACGG GGGACCCCCG GCCTGAGAGT CTTGGCACCT TAGAAATACC AACCAGGGAC 
CCGCCCCTGT CTTTCCAGTC TGATGCAGAC AGTTCTTCCA GCACCTTGGC TTCAACTAAC 
AGGGCCCCTC TTTCCACACC ATCACAGTCA CCTGACCAAA TAAATGGAGA AAGCTTCCAG 
AACACGAGCC AAAACTCCAG TTCTGCAGCC AGGGTCCAAC CTCATCAACT GTCTGAAACC 
CCAGATCACA AAGCACAGTT CCATCCCTGT CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_025000961.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856729.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X4, mRNA.

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CloneID	OBa221859
Clone ID Related Accession (Same CDS sequence)	XM_025000961.1
Accession Version	XM_025000961.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGGA GCCTCGACAC ATGTAGAGAG AGAGCGTCTG AAGGGTCCTC TCAGTGGAGG 
CATCTGGCTG CTGTGCCCTT CGCTGTGTGC CTGCAGTTTG CCCTTGGTGT AAAATCCGGC 
AGATCAAAGG CTCTGCGGGT ATCAAAAAGT AAAGATGGAA AAGAACAAAG TGAAACTGTA 
TCACCATCCG AAGATGAAAT GTTCTCCTGG CCAGGTCCCA AAACAGTTAT GTTGAAAAGA 
ACATCTCAAG GCTTTGGTTT TACATTGAGG CATTTTATTG TTTATCCCCC AGAGTCTGCA 
ATTCAGTTTT CATTTAAGGA TGAAGAAAAT GGCAACAGAG GAGGAAAACA AAGAAACCGT 
TTGGAACCCA TGGACACCAT CTTTGTTAAA CAGGTGAAAG AGGGCGGACC TGCTTTTGAA 
GCTGGATTAT GCACAGGTGA TCGAATTATA AAAGTCAATG GAGAAAGTGT TATTGGAAAG 
ACCTATTCTC AAGTAATTGC TTTAATCCAG AACAGTGATA CAACATTGGA ACTTAGTGTT 
ATGCCAAAAG ATGAAGACAT TCTCCAAGTG GCGTATTCTC AAGATGCCTA CCTGAAAGGC 
AATGAAGCCT ACAGCGGCAA TGCCCGCAAC ATCCCTGAGC CCCCACCAGT CTGCTACCCC 
TGGCCGTCAT CCGCCCCTCC CGCCATGGCG CAGCCCCCTG AACCACCTCC TCCAGACTCC 
ACAGCAAGCA AACCGCAGAC TAGCCCCCCA GTATTGACGC AGCCCGGCAG GGCATACAGG 
ATGGAAATCC AAGTGCCTCC GTCACCACCA GAGGTGGCCA AGTCCAACAC AGCAGTGTGT 
GTCTGCAATG AGAGTGTGAG GACCGTCATT GTACCTTCTG AGAAGGTTGT AGATTTATTA 
CCTAACAGAA GCAACCACAC AGTTCCCGCT CACAGAACTG AGGAAGTGAG GTATGGCCGA 
AACGAACAGC CCTCTTTCAA AACAGCGACG AGAACCACCT CACCGCCATC CTCAGTTCCC 
ACTGCCCATC TCATCCACCA GCCCGGCTCC AGGTCGTTGG AACCTTCTGG CATTTTACTT 
AAATCTGGCC ACTACGGCGG ACACTCAGAA GGTCTGGTGA GCAGATCTCC AGCTGTGGAG 
TCTCCTTCCG TAACTGTCAA CCACTACTCT CCCAACTCCC ATCAGCACAT AGACTGGAAA 
AACTATAAAA CTTACAAAGA GTATATTGAT AACCGGCGCT TGCACATGGG CTGTCGGACC 
ATTCAAGAAA GGTTAGATAG TTTAAGAGCC GCCTCTCAGA GCACAGCGGA TTATAACCAG 
GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA CGGCGTCGGA GCACCTCTCA GGACCGAGTG 
CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC GTGTCCCAGG AGAGGCTGGA GGACTCTGTG 
TTGATGATGT ATTGTCCGAG GAGTGCCTCT CAAGGTGCGC TGACATCCCC TTCTATTAGT 
TTTAGTAACC ACAGAACTCG CTCGTGGGAT TACATCGAGG GACAGGGTGA AACCTCGGAA 
AACGTCCATT CTGAATGTCC ACCGCCGGAT GCAAATGGTG AACGAAAGCA GACTTACAAG 
TGGAGTGGGT TCACCGAACA GGACGATCGT CGAGGTATTT ACGAGAGACC CAGGCAGCAA 
GAAATTCACA AATCTTTCCG AGGCTCCAAT TTTACAGTGG CTCCCAGTGT GGTGAATTCT 
GACAGCAGGA GAGTGAGCAG CAGAATCGGG GGATCAGTGT CTCAGTTTAA AAAAATTCCA 
GCAGATCTAA AACCCCTGCA GCCCAGCAGA AATTTTCAAA CTGCTTGTGG AATATCCCAG 
CCTCGAGGGA TTTCCCAAGA CAGGTCACCT CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA 
GGGCCTTCTA TGCATGTCGC GAAACCGCCC TCCAGCCAGA ACTCATTTCT TTCTATCAAA 
GACCAAAGAC CAGTAAATCA CTTGCATCAA AACAGTTTAT TGAATCAGCA GCCATGGTTA 
CGGACGGAAA GTGCCCCCGA TCACCAAGTG GACACTGGGA AGCCCCCTTC TCTTTCCGGA 
GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT GAGAATGCTA GCACTCCAGA TTTTGAGTTA 
TCCGTCTCTC AGAGGAATCA AGATTTAAAT GTACAAGAGG TGGAAATTCA GCAATCGAAT 
GCTGTAGATA ATAAAGAAAC TGTCATCCTA AGAGAAAAAC CTCCGTCTGG TCGCCAGACA 
CCGCAGCCTT TAAGGCATCA GTCTTACATC TTAGCAGTGA GTGACCAGGA GACAGGCTCA 
GACACCACCT GCTGGCTGCC TAATGATGCT CGCCGGGAGG TCCATATAAA AAGGATGGAG 
GAGAGGAAGG CCTCAAGTAC CACTCCACCT GGTGATTCCT TGGCTTCCAT CCCATTTATA 
GATGAACCTA CCAGCCCTAG CATCGATCAC GACATTTCTC ACATCCCCGC CTCTGCGGTC 
ATCTCTGCCT CCACCTCCCA GGTACCCTCC ATAGCAACGG TCCCTCCCAG CCTCACAACT 
TCAGCTCCAT TAATCCGACG TCAGCTCTCC CATGACCAGG AATCTGTTGG ACCTCCCAGC 
CTGGATGCCC AGCCCAGCTC AAAGACAGAG CGATCCAAAT CATATGATGA GGGCCTGGAT 
GATTACAGAG AAGATGCAAA ACTGTCTTTT AAACATGTAT CTAGCCTGAA GGGAATCAAG 
ATCTTAGACA GTCAAAAGTC ATCAGAAGAT TCCGGATCCA GGAAAGACTC TTCCTCAGAG 
GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG CTTCATTTCA GGCCACTCGT CACTGATAAG 
GGCAAGCGCG TTGGTGGAAG TATTCGGCCA TGGAAGCAGA TGTATGTTGT ACTTCGGGGC 
CATTCGCTGT ATCTGTACAA AGATAAACGA GAACAGACGA CTCCGTCTGA GGAGGAGCAG 
CCCATCAGTG TTAACGCCTG CTTAATAGAC ATCTCTTACA GCGAGACCAA GAGGAAGAAT 
GTCTTTCGAC TCACCACGTC TGACTGCGAG TGCCTGTTTC AGGCTGAAGA CAGGGATGAC 
ATGTTAGCCT GGATCAAAAC CATCCAGGAG AGCAGCAATT TAAACGAGGA GGACACTGGA 
GTCACTAACA GGGACCTAAT TAGTCGAAGA ATAAAAGAAT ACAACAGTCT AATGAGCAAA 
GCAGAGCCAT TGCCGAAGAC CCCTCGCCAG AGTCTCAGCA TCCGGCAGAC CCTACTTGGT 
GCTAAAGCAG AGCCTCGGAC TCAGAGTCCA CACTCTCCCA AAGAGGAGTC AGAAAGGAAG 
CTTCTTAGTA AAGATGATAC CAGTCCTCCA AAAGACAAAG GCACGTGGAG AAAAGGCATT 
CCAAGTATTA TGAGAAAGAC ATTTGAGAAA AAGCCTACTG CTACGGGAAC ATTCGGGGTC 
AGACTGGACG ACTGCCCACC AGCTCATACA AACCGGTATA TTCCATTAAT AGTTGACATA 
TGTTGTAAAT TAGTTGAAGA AAGAGGTCTG GAATATACAG GTATTTACAG AGTCCCTGGA 
AACAATGCAG CCATCTCCAG TATGCAGGAG GAGCTCAACA AGGGAATGGC TGATATTGAT 
ATACAAGATG ATAAATGGCG AGATTTGAAT GTGATCAGCA GTTTATTAAA ATCCTTCTTC 
AGAAAACTCC CAGAACCTCT CTTCACGAAT GATAAATACG CCGACTTTAT TGAAGCCAAT 
CGTAAGGAAG ATCCTCTAGA TCGTCTGAAA ACATTAAAAA GACTAATTCA TGATTTGCCC 
GAACATCATT ATGAAACACT CAAGTTTCTT TCGGCTCATC TGAAGACAGT AGCAGAAAAT 
TCAGAAAAAA ATAAGATGGA ACCAAGAAAC CTAGCCATAG TGTTTGGTCC AACTCTTGTG 
AGAACCTCCG AAGATAACAT GACACACATG GTCACTCACA TGCCAGATCA GTACAAGATC 
GTAGAGACAC TCATCCAGCA CCATGACTGG TTTTTCACAG AAGAAGGTGC TGAAGAACCT 
CTTACAACAG TGCAGGAGGA AAACACAGTA GACTCCCAGC CAGTGCCAAA CATAGATCAT 
TTACTCACCA ACATTGGAAG GACAGGCGTC TCCCCTGGAG ATGTATCAGA TTCAGCTACT 
AGTGACTCAA CAAAATCTAA GGGTTCTTGG GGATCCGGAA AAGACCAGTA CAGCAAGGAC 
CTGCTTGTAT CCTCCATCTT CGCGGCTGCC AGTCGCAAGA GGAAAAAGCC AAAAGAAAAA 
GCACAACCCA GCAGCTCGGA AGACGAGCTG GACAATGTGT TTTTCAAGAA AGAAAACTCA 
GAGCAGGGTC ACCACGACAT TAAAGAAGAG TCCAAAAAAG AGAGTGAGAC ACCAGGCCGG 
AAACAAAGGA CCGTTGCTCC CAAAGAAAAC AACACGAAGA AAGACAGCAG CGGAGGTGGC 
AGAGCTGAGC AGAGGGCCCC GCGTGGGGAG AGCTTGGAGC CCCCGGCCCC CCAAAGCACC 
AAGCAGAGCC GGTCACCCAC CCTGAGCTGT CGCCTCGCCG TCCTGAGAGA GAGCCCCAGG 
GCCCTCGCAG CCCAGAAGAC CTCCCACCTG GAAGACACGG GGTCCGACTC CAGCACCCCA 
CTCAGCACTC ACGCCCCGGC TCCCCCGGCA AGCTTTCCCA CCAAGAAACC TCCCAGCCCC 
GAGCCCAAGC ACAGCGAGCT CCTGGTCAGT GTCGGCAGCA TCACCTCCGA CCACGCCGGC 
ACACCGTCTG CTCCCTACCT GGCCGGCCTC GACTCCAGCC GGCTGAGCCC CGAGGTGCAG 
TCCATGGCCG AGAGCCGGGG ATTTGAAGCT GATGACGAGA GGAGCGAGCT GGTCAGCGAG 
GGCCGGCCAG TGGAGACAGA CAGCGAGAGC GACTTTCCGG TCTTCCCCGC CGCCCCGGCC 
TCGGACAGGC TCTTCCGAGG AAAACTCCAA GAGGCCGCGA GGACCAGCCG GAGAAACTCA 
GAAGGCAGTG AGGTCAGCTG CACGGAAGGA AGTTTAACAC CAAGTTTAGA AAGCCGGAGA 
CAGCTCTTCA GCTCCCATAA ACTGATCGAG TGTGACACTC TGTCCAGGAA GAAATCCGCC 
AGGTTCAAGT CAGACAGTGG GAGTCTAGGA GATGCCAAGA ATGAGAAAGA AGCCCCTTCC 
ATCACCAAAG TGTTCGACGT TATGAAAAAA GGAAAATCCA CCGGAAATTT ACTGACACCA 
CCAGCCAGAA GCGAATCGGA CAAACAGGAA CCCACTTGGA AAACAAAAAT AGCCGATCGG 
TTAAAACTGA GGCCCAAAGC CCCGGCCGAT GACATGTTTG GAGTAGGAAG TCAAAAAACC 
ATCGCCGAAC CTGCCAAAAG GAAAAACATC AAACGCAGAC ACACGCTGGG TGGGCACAGG 
GATGCTACTG AAATGAGTGT TCTGAATTTC TGGAAAGCCC AGGAGCACAG CGGGGACAGA 
GAATCCGAAC TTTCAGCTGT GAATCGATTG AAGCCCAAAT GCTCCGCCCA GGACCTGTCC 
ATCTCAGACT GGCTGGCCAG GGAGCGGCTA CGCACCAGTA TGACTGACCT GAGCAGAGGG 
GACACGGGGG ACCCCCGGCC TGAGAGTCTT GGCACCTTAG AAATACCAAC CAGGGACCCG 
CCCCTGTCTT TCCAGTCTGA TGCAGACAGT TCTTCCAGCA CCTTGGCTTC AACTAACAGG 
GCCCCTCTTT CCACACCATC ACAGTCACCT GACCAAATAA ATGGAGAAAG CTTCCAGAAC 
ACGAGCCAAA ACTCCAGTTC TGCAGCCAGG GTCCAACCTC ATCAACTGTC TGAAACCCCA 
GATCACAAAG CACAGTTCCA TCCCTGTCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856729.1
CDS	73..5985
Translation	MVGSLDTCRERASEGSSQWRHLAAVPFAVCLQFALGVKSGRSKALRVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_025000961.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000961.1

ORF Insert Sequence:

ATGGTGGGGA GCCTCGACAC ATGTAGAGAG AGAGCGTCTG AAGGGTCCTC TCAGTGGAGG 
CATCTGGCTG CTGTGCCCTT CGCTGTGTGC CTGCAGTTTG CCCTTGGTGT AAAATCCGGC 
AGATCAAAGG CTCTGCGGGT ATCAAAAAGT AAAGATGGAA AAGAACAAAG TGAAACTGTA 
TCACCATCCG AAGATGAAAT GTTCTCCTGG CCAGGTCCCA AAACAGTTAT GTTGAAAAGA 
ACATCTCAAG GCTTTGGTTT TACATTGAGG CATTTTATTG TTTATCCCCC AGAGTCTGCA 
ATTCAGTTTT CATTTAAGGA TGAAGAAAAT GGCAACAGAG GAGGAAAACA AAGAAACCGT 
TTGGAACCCA TGGACACCAT CTTTGTTAAA CAGGTGAAAG AGGGCGGACC TGCTTTTGAA 
GCTGGATTAT GCACAGGTGA TCGAATTATA AAAGTCAATG GAGAAAGTGT TATTGGAAAG 
ACCTATTCTC AAGTAATTGC TTTAATCCAG AACAGTGATA CAACATTGGA ACTTAGTGTT 
ATGCCAAAAG ATGAAGACAT TCTCCAAGTG GCGTATTCTC AAGATGCCTA CCTGAAAGGC 
AATGAAGCCT ACAGCGGCAA TGCCCGCAAC ATCCCTGAGC CCCCACCAGT CTGCTACCCC 
TGGCCGTCAT CCGCCCCTCC CGCCATGGCG CAGCCCCCTG AACCACCTCC TCCAGACTCC 
ACAGCAAGCA AACCGCAGAC TAGCCCCCCA GTATTGACGC AGCCCGGCAG GGCATACAGG 
ATGGAAATCC AAGTGCCTCC GTCACCACCA GAGGTGGCCA AGTCCAACAC AGCAGTGTGT 
GTCTGCAATG AGAGTGTGAG GACCGTCATT GTACCTTCTG AGAAGGTTGT AGATTTATTA 
CCTAACAGAA GCAACCACAC AGTTCCCGCT CACAGAACTG AGGAAGTGAG GTATGGCCGA 
AACGAACAGC CCTCTTTCAA AACAGCGACG AGAACCACCT CACCGCCATC CTCAGTTCCC 
ACTGCCCATC TCATCCACCA GCCCGGCTCC AGGTCGTTGG AACCTTCTGG CATTTTACTT 
AAATCTGGCC ACTACGGCGG ACACTCAGAA GGTCTGGTGA GCAGATCTCC AGCTGTGGAG 
TCTCCTTCCG TAACTGTCAA CCACTACTCT CCCAACTCCC ATCAGCACAT AGACTGGAAA 
AACTATAAAA CTTACAAAGA GTATATTGAT AACCGGCGCT TGCACATGGG CTGTCGGACC 
ATTCAAGAAA GGTTAGATAG TTTAAGAGCC GCCTCTCAGA GCACAGCGGA TTATAACCAG 
GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA CGGCGTCGGA GCACCTCTCA GGACCGAGTG 
CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC GTGTCCCAGG AGAGGCTGGA GGACTCTGTG 
TTGATGATGT ATTGTCCGAG GAGTGCCTCT CAAGGTGCGC TGACATCCCC TTCTATTAGT 
TTTAGTAACC ACAGAACTCG CTCGTGGGAT TACATCGAGG GACAGGGTGA AACCTCGGAA 
AACGTCCATT CTGAATGTCC ACCGCCGGAT GCAAATGGTG AACGAAAGCA GACTTACAAG 
TGGAGTGGGT TCACCGAACA GGACGATCGT CGAGGTATTT ACGAGAGACC CAGGCAGCAA 
GAAATTCACA AATCTTTCCG AGGCTCCAAT TTTACAGTGG CTCCCAGTGT GGTGAATTCT 
GACAGCAGGA GAGTGAGCAG CAGAATCGGG GGATCAGTGT CTCAGTTTAA AAAAATTCCA 
GCAGATCTAA AACCCCTGCA GCCCAGCAGA AATTTTCAAA CTGCTTGTGG AATATCCCAG 
CCTCGAGGGA TTTCCCAAGA CAGGTCACCT CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA 
GGGCCTTCTA TGCATGTCGC GAAACCGCCC TCCAGCCAGA ACTCATTTCT TTCTATCAAA 
GACCAAAGAC CAGTAAATCA CTTGCATCAA AACAGTTTAT TGAATCAGCA GCCATGGTTA 
CGGACGGAAA GTGCCCCCGA TCACCAAGTG GACACTGGGA AGCCCCCTTC TCTTTCCGGA 
GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT GAGAATGCTA GCACTCCAGA TTTTGAGTTA 
TCCGTCTCTC AGAGGAATCA AGATTTAAAT GTACAAGAGG TGGAAATTCA GCAATCGAAT 
GCTGTAGATA ATAAAGAAAC TGTCATCCTA AGAGAAAAAC CTCCGTCTGG TCGCCAGACA 
CCGCAGCCTT TAAGGCATCA GTCTTACATC TTAGCAGTGA GTGACCAGGA GACAGGCTCA 
GACACCACCT GCTGGCTGCC TAATGATGCT CGCCGGGAGG TCCATATAAA AAGGATGGAG 
GAGAGGAAGG CCTCAAGTAC CACTCCACCT GGTGATTCCT TGGCTTCCAT CCCATTTATA 
GATGAACCTA CCAGCCCTAG CATCGATCAC GACATTTCTC ACATCCCCGC CTCTGCGGTC 
ATCTCTGCCT CCACCTCCCA GGTACCCTCC ATAGCAACGG TCCCTCCCAG CCTCACAACT 
TCAGCTCCAT TAATCCGACG TCAGCTCTCC CATGACCAGG AATCTGTTGG ACCTCCCAGC 
CTGGATGCCC AGCCCAGCTC AAAGACAGAG CGATCCAAAT CATATGATGA GGGCCTGGAT 
GATTACAGAG AAGATGCAAA ACTGTCTTTT AAACATGTAT CTAGCCTGAA GGGAATCAAG 
ATCTTAGACA GTCAAAAGTC ATCAGAAGAT TCCGGATCCA GGAAAGACTC TTCCTCAGAG 
GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG CTTCATTTCA GGCCACTCGT CACTGATAAG 
GGCAAGCGCG TTGGTGGAAG TATTCGGCCA TGGAAGCAGA TGTATGTTGT ACTTCGGGGC 
CATTCGCTGT ATCTGTACAA AGATAAACGA GAACAGACGA CTCCGTCTGA GGAGGAGCAG 
CCCATCAGTG TTAACGCCTG CTTAATAGAC ATCTCTTACA GCGAGACCAA GAGGAAGAAT 
GTCTTTCGAC TCACCACGTC TGACTGCGAG TGCCTGTTTC AGGCTGAAGA CAGGGATGAC 
ATGTTAGCCT GGATCAAAAC CATCCAGGAG AGCAGCAATT TAAACGAGGA GGACACTGGA 
GTCACTAACA GGGACCTAAT TAGTCGAAGA ATAAAAGAAT ACAACAGTCT AATGAGCAAA 
GCAGAGCCAT TGCCGAAGAC CCCTCGCCAG AGTCTCAGCA TCCGGCAGAC CCTACTTGGT 
GCTAAAGCAG AGCCTCGGAC TCAGAGTCCA CACTCTCCCA AAGAGGAGTC AGAAAGGAAG 
CTTCTTAGTA AAGATGATAC CAGTCCTCCA AAAGACAAAG GCACGTGGAG AAAAGGCATT 
CCAAGTATTA TGAGAAAGAC ATTTGAGAAA AAGCCTACTG CTACGGGAAC ATTCGGGGTC 
AGACTGGACG ACTGCCCACC AGCTCATACA AACCGGTATA TTCCATTAAT AGTTGACATA 
TGTTGTAAAT TAGTTGAAGA AAGAGGTCTG GAATATACAG GTATTTACAG AGTCCCTGGA 
AACAATGCAG CCATCTCCAG TATGCAGGAG GAGCTCAACA AGGGAATGGC TGATATTGAT 
ATACAAGATG ATAAATGGCG AGATTTGAAT GTGATCAGCA GTTTATTAAA ATCCTTCTTC 
AGAAAACTCC CAGAACCTCT CTTCACGAAT GATAAATACG CCGACTTTAT TGAAGCCAAT 
CGTAAGGAAG ATCCTCTAGA TCGTCTGAAA ACATTAAAAA GACTAATTCA TGATTTGCCC 
GAACATCATT ATGAAACACT CAAGTTTCTT TCGGCTCATC TGAAGACAGT AGCAGAAAAT 
TCAGAAAAAA ATAAGATGGA ACCAAGAAAC CTAGCCATAG TGTTTGGTCC AACTCTTGTG 
AGAACCTCCG AAGATAACAT GACACACATG GTCACTCACA TGCCAGATCA GTACAAGATC 
GTAGAGACAC TCATCCAGCA CCATGACTGG TTTTTCACAG AAGAAGGTGC TGAAGAACCT 
CTTACAACAG TGCAGGAGGA AAACACAGTA GACTCCCAGC CAGTGCCAAA CATAGATCAT 
TTACTCACCA ACATTGGAAG GACAGGCGTC TCCCCTGGAG ATGTATCAGA TTCAGCTACT 
AGTGACTCAA CAAAATCTAA GGGTTCTTGG GGATCCGGAA AAGACCAGTA CAGCAAGGAC 
CTGCTTGTAT CCTCCATCTT CGCGGCTGCC AGTCGCAAGA GGAAAAAGCC AAAAGAAAAA 
GCACAACCCA GCAGCTCGGA AGACGAGCTG GACAATGTGT TTTTCAAGAA AGAAAACTCA 
GAGCAGGGTC ACCACGACAT TAAAGAAGAG TCCAAAAAAG AGAGTGAGAC ACCAGGCCGG 
AAACAAAGGA CCGTTGCTCC CAAAGAAAAC AACACGAAGA AAGACAGCAG CGGAGGTGGC 
AGAGCTGAGC AGAGGGCCCC GCGTGGGGAG AGCTTGGAGC CCCCGGCCCC CCAAAGCACC 
AAGCAGAGCC GGTCACCCAC CCTGAGCTGT CGCCTCGCCG TCCTGAGAGA GAGCCCCAGG 
GCCCTCGCAG CCCAGAAGAC CTCCCACCTG GAAGACACGG GGTCCGACTC CAGCACCCCA 
CTCAGCACTC ACGCCCCGGC TCCCCCGGCA AGCTTTCCCA CCAAGAAACC TCCCAGCCCC 
GAGCCCAAGC ACAGCGAGCT CCTGGTCAGT GTCGGCAGCA TCACCTCCGA CCACGCCGGC 
ACACCGTCTG CTCCCTACCT GGCCGGCCTC GACTCCAGCC GGCTGAGCCC CGAGGTGCAG 
TCCATGGCCG AGAGCCGGGG ATTTGAAGCT GATGACGAGA GGAGCGAGCT GGTCAGCGAG 
GGCCGGCCAG TGGAGACAGA CAGCGAGAGC GACTTTCCGG TCTTCCCCGC CGCCCCGGCC 
TCGGACAGGC TCTTCCGAGG AAAACTCCAA GAGGCCGCGA GGACCAGCCG GAGAAACTCA 
GAAGGCAGTG AGGTCAGCTG CACGGAAGGA AGTTTAACAC CAAGTTTAGA AAGCCGGAGA 
CAGCTCTTCA GCTCCCATAA ACTGATCGAG TGTGACACTC TGTCCAGGAA GAAATCCGCC 
AGGTTCAAGT CAGACAGTGG GAGTCTAGGA GATGCCAAGA ATGAGAAAGA AGCCCCTTCC 
ATCACCAAAG TGTTCGACGT TATGAAAAAA GGAAAATCCA CCGGAAATTT ACTGACACCA 
CCAGCCAGAA GCGAATCGGA CAAACAGGAA CCCACTTGGA AAACAAAAAT AGCCGATCGG 
TTAAAACTGA GGCCCAAAGC CCCGGCCGAT GACATGTTTG GAGTAGGAAG TCAAAAAACC 
ATCGCCGAAC CTGCCAAAAG GAAAAACATC AAACGCAGAC ACACGCTGGG TGGGCACAGG 
GATGCTACTG AAATGAGTGT TCTGAATTTC TGGAAAGCCC AGGAGCACAG CGGGGACAGA 
GAATCCGAAC TTTCAGCTGT GAATCGATTG AAGCCCAAAT GCTCCGCCCA GGACCTGTCC 
ATCTCAGACT GGCTGGCCAG GGAGCGGCTA CGCACCAGTA TGACTGACCT GAGCAGAGGG 
GACACGGGGG ACCCCCGGCC TGAGAGTCTT GGCACCTTAG AAATACCAAC CAGGGACCCG 
CCCCTGTCTT TCCAGTCTGA TGCAGACAGT TCTTCCAGCA CCTTGGCTTC AACTAACAGG 
GCCCCTCTTT CCACACCATC ACAGTCACCT GACCAAATAA ATGGAGAAAG CTTCCAGAAC 
ACGAGCCAAA ACTCCAGTTC TGCAGCCAGG GTCCAACCTC ATCAACTGTC TGAAACCCCA 
GATCACAAAG CACAGTTCCA TCCCTGTCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_005194286.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005194343.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X1, mRNA.

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CloneID	OBa07464
Clone ID Related Accession (Same CDS sequence)	XM_005194286.3 , XM_005214298.3 , XM_025000962.1 , XM_005214297.4 , XM_005214297.3 , XM_005194287.3 , XM_005214298.4 , XM_015465939.1 , XM_015474021.2 , XM_015474021.1
Accession Version	XM_005194286.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646129.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194286.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005194343.1
CDS	539..6427
Translation	MMATRRTGLPEGDGDTKLKACGSPTCEVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_005194286.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005194286.3

ORF Insert Sequence:

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_005214298.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214355.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.

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CloneID	OBa07464
Clone ID Related Accession (Same CDS sequence)	XM_005194286.3 , XM_005214298.3 , XM_025000962.1 , XM_005214297.4 , XM_005214297.3 , XM_005194287.3 , XM_005214298.4 , XM_015465939.1 , XM_015474021.2 , XM_015474021.1
Accession Version	XM_005214298.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005214298.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214355.1
CDS	633..6521
Translation	MMATRRTGLPEGDGDTKLKACGSPTCEVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_005214298.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214298.3

ORF Insert Sequence:

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_025000962.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856730.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X8, mRNA.

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CloneID	OBa07464
Clone ID Related Accession (Same CDS sequence)	XM_005194286.3 , XM_005214298.3 , XM_025000962.1 , XM_005214297.4 , XM_005214297.3 , XM_005194287.3 , XM_005214298.4 , XM_015465939.1 , XM_015474021.2 , XM_015474021.1
Accession Version	XM_025000962.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856730.1
CDS	463..6351
Translation	MMATRRTGLPEGDGDTKLKACGSPTCEVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_025000962.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000962.1

ORF Insert Sequence:

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_005214297.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214354.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.

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CloneID	OBa07464
Clone ID Related Accession (Same CDS sequence)	XM_005194286.3 , XM_005214298.3 , XM_025000962.1 , XM_005214297.4 , XM_005214297.3 , XM_005194287.3 , XM_005214298.4 , XM_015465939.1 , XM_015474021.2 , XM_015474021.1
Accession Version	XM_005214297.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005214297.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214354.1
CDS	531..6419
Translation	MMATRRTGLPEGDGDTKLKACGSPTCEVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_005214297.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214297.4

ORF Insert Sequence:

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_005214297.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214354.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X1, mRNA.

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CloneID	OBa07464
Clone ID Related Accession (Same CDS sequence)	XM_005194286.3 , XM_005214298.3 , XM_025000962.1 , XM_005214297.4 , XM_005214297.3 , XM_005194287.3 , XM_005214298.4 , XM_015465939.1 , XM_015474021.2 , XM_015474021.1
Accession Version	XM_005214297.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005214297.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214354.1
CDS	539..6427
Translation	MMATRRTGLPEGDGDTKLKACGSPTCEVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_005214297.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214297.3

ORF Insert Sequence:

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_005194287.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005194344.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.

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CloneID	OBa07464
Clone ID Related Accession (Same CDS sequence)	XM_005194286.3 , XM_005214298.3 , XM_025000962.1 , XM_005214297.4 , XM_005214297.3 , XM_005194287.3 , XM_005214298.4 , XM_015465939.1 , XM_015474021.2 , XM_015474021.1
Accession Version	XM_005194287.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646129.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194287.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005194344.1
CDS	633..6521
Translation	MMATRRTGLPEGDGDTKLKACGSPTCEVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_005194287.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005194287.3

ORF Insert Sequence:

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_005214298.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214355.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X6, mRNA.

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CloneID	OBa07464
Clone ID Related Accession (Same CDS sequence)	XM_005194286.3 , XM_005214298.3 , XM_025000962.1 , XM_005214297.4 , XM_005214297.3 , XM_005194287.3 , XM_005214298.4 , XM_015465939.1 , XM_015474021.2 , XM_015474021.1
Accession Version	XM_005214298.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005214298.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214355.1
CDS	712..6600
Translation	MMATRRTGLPEGDGDTKLKACGSPTCEVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_005214298.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214298.4

ORF Insert Sequence:

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_015465939.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321425.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X3, mRNA.

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CloneID	OBa07464
Clone ID Related Accession (Same CDS sequence)	XM_005194286.3 , XM_005214298.3 , XM_025000962.1 , XM_005214297.4 , XM_005214297.3 , XM_005194287.3 , XM_005214298.4 , XM_015465939.1 , XM_015474021.2 , XM_015474021.1
Accession Version	XM_015465939.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646129.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321425.1
CDS	374..6262
Translation	MMATRRTGLPEGDGDTKLKACGSPTCEVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_015465939.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465939.1

ORF Insert Sequence:

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_015474021.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329507.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa07464
Clone ID Related Accession (Same CDS sequence)	XM_005194286.3 , XM_005214298.3 , XM_025000962.1 , XM_005214297.4 , XM_005214297.3 , XM_005194287.3 , XM_005214298.4 , XM_015465939.1 , XM_015474021.2 , XM_015474021.1
Accession Version	XM_015474021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474021.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329507.1
CDS	414..6302
Translation	MMATRRTGLPEGDGDTKLKACGSPTCEVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_015474021.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474021.2

ORF Insert Sequence:

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_015474021.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329507.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa07464
Clone ID Related Accession (Same CDS sequence)	XM_005194286.3 , XM_005214298.3 , XM_025000962.1 , XM_005214297.4 , XM_005214297.3 , XM_005194287.3 , XM_005214298.4 , XM_015465939.1 , XM_015474021.2 , XM_015474021.1
Accession Version	XM_015474021.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329507.1
CDS	374..6262
Translation	MMATRRTGLPEGDGDTKLKACGSPTCEVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_015474021.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474021.1

ORF Insert Sequence:

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AGCAAAGCAG AGCCATTGCC GAAGACCCCT 
CGCCAGAGTC TCAGCATCCG GCAGACCCTA CTTGGTGCTA AAGCAGAGCC TCGGACTCAG 
AGTCCACACT CTCCCAAAGA GGAGTCAGAA AGGAAGCTTC TTAGTAAAGA TGATACCAGT 
CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA GGCATTCCAA GTATTATGAG AAAGACATTT 
GAGAAAAAGC CTACTGCTAC GGGAACATTC GGGGTCAGAC TGGACGACTG CCCACCAGCT 
CATACAAACC GGTATATTCC ATTAATAGTT GACATATGTT GTAAATTAGT TGAAGAAAGA 
GGTCTGGAAT ATACAGGTAT TTACAGAGTC CCTGGAAACA ATGCAGCCAT CTCCAGTATG 
CAGGAGGAGC TCAACAAGGG AATGGCTGAT ATTGATATAC AAGATGATAA ATGGCGAGAT 
TTGAATGTGA TCAGCAGTTT ATTAAAATCC TTCTTCAGAA AACTCCCAGA ACCTCTCTTC 
ACGAATGATA AATACGCCGA CTTTATTGAA GCCAATCGTA AGGAAGATCC TCTAGATCGT 
CTGAAAACAT TAAAAAGACT AATTCATGAT TTGCCCGAAC ATCATTATGA AACACTCAAG 
TTTCTTTCGG CTCATCTGAA GACAGTAGCA GAAAATTCAG AAAAAAATAA GATGGAACCA 
AGAAACCTAG CCATAGTGTT TGGTCCAACT CTTGTGAGAA CCTCCGAAGA TAACATGACA 
CACATGGTCA CTCACATGCC AGATCAGTAC AAGATCGTAG AGACACTCAT CCAGCACCAT 
GACTGGTTTT TCACAGAAGA AGGTGCTGAA GAACCTCTTA CAACAGTGCA GGAGGAAAAC 
ACAGTAGACT CCCAGCCAGT GCCAAACATA GATCATTTAC TCACCAACAT TGGAAGGACA 
GGCGTCTCCC CTGGAGATGT ATCAGATTCA GCTACTAGTG ACTCAACAAA ATCTAAGGGT 
TCTTGGGGAT CCGGAAAAGA CCAGTACAGC AAGGACCTGC TTGTATCCTC CATCTTCGCG 
GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA GAAAAAGCAC AACCCAGCAG CTCGGAAGAC 
GAGCTGGACA ATGTGTTTTT CAAGAAAGAA AACTCAGAGC AGGGTCACCA CGACATTAAA 
GAAGAGTCCA AAAAAGAGAG TGAGACACCA GGCCGGAAAC AAAGGACCGT TGCTCCCAAA 
GAAAACAACA CGAAGAAAGA CAGCAGCGGA GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT 
GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA AGCACCAAGC AGAGCCGGTC ACCCACCCTG 
AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC CCCAGGGCCC TCGCAGCCCA GAAGACCTCC 
CACCTGGAAG ACACGGGGTC CGACTCCAGC ACCCCACTCA GCACTCACGC CCCGGCTCCC 
CCGGCAAGCT TTCCCACCAA GAAACCTCCC AGCCCCGAGC CCAAGCACAG CGAGCTCCTG 
GTCAGTGTCG GCAGCATCAC CTCCGACCAC GCCGGCACAC CGTCTGCTCC CTACCTGGCC 
GGCCTCGACT CCAGCCGGCT GAGCCCCGAG GTGCAGTCCA TGGCCGAGAG CCGGGGATTT 
GAAGCTGATG ACGAGAGGAG CGAGCTGGTC AGCGAGGGCC GGCCAGTGGA GACAGACAGC 
GAGAGCGACT TTCCGGTCTT CCCCGCCGCC CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA 
CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA AACTCAGAAG GCAGTGAGGT CAGCTGCACG 
GAAGGAAGTT TAACACCAAG TTTAGAAAGC CGGAGACAGC TCTTCAGCTC CCATAAACTG 
ATCGAGTGTG ACACTCTGTC CAGGAAGAAA TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCC CCTTCCATCA CCAAAGTGTT CGACGTTATG 
AAAAAAGGAA AATCCACCGG AAATTTACTG ACACCACCAG CCAGAAGCGA ATCGGACAAA 
CAGGAACCCA CTTGGAAAAC AAAAATAGCC GATCGGTTAA AACTGAGGCC CAAAGCCCCG 
GCCGATGACA TGTTTGGAGT AGGAAGTCAA AAAACCATCG CCGAACCTGC CAAAAGGAAA 
AACATCAAAC GCAGACACAC GCTGGGTGGG CACAGGGATG CTACTGAAAT GAGTGTTCTG 
AATTTCTGGA AAGCCCAGGA GCACAGCGGG GACAGAGAAT CCGAACTTTC AGCTGTGAAT 
CGATTGAAGC CCAAATGCTC CGCCCAGGAC CTGTCCATCT CAGACTGGCT GGCCAGGGAG 
CGGCTACGCA CCAGTATGAC TGACCTGAGC AGAGGGGACA CGGGGGACCC CCGGCCTGAG 
AGTCTTGGCA CCTTAGAAAT ACCAACCAGG GACCCGCCCC TGTCTTTCCA GTCTGATGCA 
GACAGTTCTT CCAGCACCTT GGCTTCAACT AACAGGGCCC CTCTTTCCAC ACCATCACAG 
TCACCTGACC AAATAAATGG AGAAAGCTTC CAGAACACGA GCCAAAACTC CAGTTCTGCA 
GCCAGGGTCC AACCTCATCA ACTGTCTGAA ACCCCAGATC ACAAAGCACA GTTCCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_005214301.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214358.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X4, mRNA.

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CloneID	OBa07465
Clone ID Related Accession (Same CDS sequence)	XM_005214301.3 , XM_005194290.3
Accession Version	XM_005214301.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005214301.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AAAGCAGAGC CATTGCCGAA GACCCCTCGC 
CAGAGTCTCA GCATCCGGCA GACCCTACTT GGTGCTAAAG CAGAGCCTCG GACTCAGAGT 
CCACACTCTC CCAAAGAGGA GTCAGAAAGG AAGCTTCTTA GTAAAGATGA TACCAGTCCT 
CCAAAAGACA AAGGCACGTG GAGAAAAGGC ATTCCAAGTA TTATGAGAAA GACATTTGAG 
AAAAAGCCTA CTGCTACGGG AACATTCGGG GTCAGACTGG ACGACTGCCC ACCAGCTCAT 
ACAAACCGGT ATATTCCATT AATAGTTGAC ATATGTTGTA AATTAGTTGA AGAAAGAGGT 
CTGGAATATA CAGGTATTTA CAGAGTCCCT GGAAACAATG CAGCCATCTC CAGTATGCAG 
GAGGAGCTCA ACAAGGGAAT GGCTGATATT GATATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATCA GCAGTTTATT AAAATCCTTC TTCAGAAAAC TCCCAGAACC TCTCTTCACG 
AATGATAAAT ACGCCGACTT TATTGAAGCC AATCGTAAGG AAGATCCTCT AGATCGTCTG 
AAAACATTAA AAAGACTAAT TCATGATTTG CCCGAACATC ATTATGAAAC ACTCAAGTTT 
CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA AATTCAGAAA AAAATAAGAT GGAACCAAGA 
AACCTAGCCA TAGTGTTTGG TCCAACTCTT GTGAGAACCT CCGAAGATAA CATGACACAC 
ATGGTCACTC ACATGCCAGA TCAGTACAAG ATCGTAGAGA CACTCATCCA GCACCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA CCTCTTACAA CAGTGCAGGA GGAAAACACA 
GTAGACTCCC AGCCAGTGCC AAACATAGAT CATTTACTCA CCAACATTGG AAGGACAGGC 
GTCTCCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CAACAAAATC TAAGGGTTCT 
TGGGGATCCG GAAAAGACCA GTACAGCAAG GACCTGCTTG TATCCTCCAT CTTCGCGGCT 
GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA AAAGCACAAC CCAGCAGCTC GGAAGACGAG 
CTGGACAATG TGTTTTTCAA GAAAGAAAAC TCAGAGCAGG GTCACCACGA CATTAAAGAA 
GAGTCCAAAA AAGAGAGTGA GACACCAGGC CGGAAACAAA GGACCGTTGC TCCCAAAGAA 
AACAACACGA AGAAAGACAG CAGCGGAGGT GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG 
GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC ACCAAGCAGA GCCGGTCACC CACCCTGAGC 
TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC AGGGCCCTCG CAGCCCAGAA GACCTCCCAC 
CTGGAAGACA CGGGGTCCGA CTCCAGCACC CCACTCAGCA CTCACGCCCC GGCTCCCCCG 
GCAAGCTTTC CCACCAAGAA ACCTCCCAGC CCCGAGCCCA AGCACAGCGA GCTCCTGGTC 
AGTGTCGGCA GCATCACCTC CGACCACGCC GGCACACCGT CTGCTCCCTA CCTGGCCGGC 
CTCGACTCCA GCCGGCTGAG CCCCGAGGTG CAGTCCATGG CCGAGAGCCG GGGATTTGAA 
GCTGATGACG AGAGGAGCGA GCTGGTCAGC GAGGGCCGGC CAGTGGAGAC AGACAGCGAG 
AGCGACTTTC CGGTCTTCCC CGCCGCCCCG GCCTCGGACA GGCTCTTCCG AGGAAAACTC 
CAAGAGGCCG CGAGGACCAG CCGGAGAAAC TCAGAAGGCA GTGAGGTCAG CTGCACGGAA 
GGAAGTTTAA CACCAAGTTT AGAAAGCCGG AGACAGCTCT TCAGCTCCCA TAAACTGATC 
GAGTGTGACA CTCTGTCCAG GAAGAAATCC GCCAGGTTCA AGTCAGACAG TGGGAGTCTA 
GGAGATGCCA AGAATGAGAA AGAAGCCCCT TCCATCACCA AAGTGTTCGA CGTTATGAAA 
AAAGGAAAAT CCACCGGAAA TTTACTGACA CCACCAGCCA GAAGCGAATC GGACAAACAG 
GAACCCACTT GGAAAACAAA AATAGCCGAT CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC 
GATGACATGT TTGGAGTAGG AAGTCAAAAA ACCATCGCCG AACCTGCCAA AAGGAAAAAC 
ATCAAACGCA GACACACGCT GGGTGGGCAC AGGGATGCTA CTGAAATGAG TGTTCTGAAT 
TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC AGAGAATCCG AACTTTCAGC TGTGAATCGA 
TTGAAGCCCA AATGCTCCGC CCAGGACCTG TCCATCTCAG ACTGGCTGGC CAGGGAGCGG 
CTACGCACCA GTATGACTGA CCTGAGCAGA GGGGACACGG GGGACCCCCG GCCTGAGAGT 
CTTGGCACCT TAGAAATACC AACCAGGGAC CCGCCCCTGT CTTTCCAGTC TGATGCAGAC 
AGTTCTTCCA GCACCTTGGC TTCAACTAAC AGGGCCCCTC TTTCCACACC ATCACAGTCA 
CCTGACCAAA TAAATGGAGA AAGCTTCCAG AACACGAGCC AAAACTCCAG TTCTGCAGCC 
AGGGTCCAAC CTCATCAACT GTCTGAAACC CCAGATCACA AAGCACAGTT CCATCCCTGT 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214358.1
CDS	539..6424
Translation	MMATRRTGLPEGDGDTKLKACGSPTCEVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_005214301.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214301.3

ORF Insert Sequence:

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AAAGCAGAGC CATTGCCGAA GACCCCTCGC 
CAGAGTCTCA GCATCCGGCA GACCCTACTT GGTGCTAAAG CAGAGCCTCG GACTCAGAGT 
CCACACTCTC CCAAAGAGGA GTCAGAAAGG AAGCTTCTTA GTAAAGATGA TACCAGTCCT 
CCAAAAGACA AAGGCACGTG GAGAAAAGGC ATTCCAAGTA TTATGAGAAA GACATTTGAG 
AAAAAGCCTA CTGCTACGGG AACATTCGGG GTCAGACTGG ACGACTGCCC ACCAGCTCAT 
ACAAACCGGT ATATTCCATT AATAGTTGAC ATATGTTGTA AATTAGTTGA AGAAAGAGGT 
CTGGAATATA CAGGTATTTA CAGAGTCCCT GGAAACAATG CAGCCATCTC CAGTATGCAG 
GAGGAGCTCA ACAAGGGAAT GGCTGATATT GATATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATCA GCAGTTTATT AAAATCCTTC TTCAGAAAAC TCCCAGAACC TCTCTTCACG 
AATGATAAAT ACGCCGACTT TATTGAAGCC AATCGTAAGG AAGATCCTCT AGATCGTCTG 
AAAACATTAA AAAGACTAAT TCATGATTTG CCCGAACATC ATTATGAAAC ACTCAAGTTT 
CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA AATTCAGAAA AAAATAAGAT GGAACCAAGA 
AACCTAGCCA TAGTGTTTGG TCCAACTCTT GTGAGAACCT CCGAAGATAA CATGACACAC 
ATGGTCACTC ACATGCCAGA TCAGTACAAG ATCGTAGAGA CACTCATCCA GCACCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA CCTCTTACAA CAGTGCAGGA GGAAAACACA 
GTAGACTCCC AGCCAGTGCC AAACATAGAT CATTTACTCA CCAACATTGG AAGGACAGGC 
GTCTCCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CAACAAAATC TAAGGGTTCT 
TGGGGATCCG GAAAAGACCA GTACAGCAAG GACCTGCTTG TATCCTCCAT CTTCGCGGCT 
GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA AAAGCACAAC CCAGCAGCTC GGAAGACGAG 
CTGGACAATG TGTTTTTCAA GAAAGAAAAC TCAGAGCAGG GTCACCACGA CATTAAAGAA 
GAGTCCAAAA AAGAGAGTGA GACACCAGGC CGGAAACAAA GGACCGTTGC TCCCAAAGAA 
AACAACACGA AGAAAGACAG CAGCGGAGGT GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG 
GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC ACCAAGCAGA GCCGGTCACC CACCCTGAGC 
TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC AGGGCCCTCG CAGCCCAGAA GACCTCCCAC 
CTGGAAGACA CGGGGTCCGA CTCCAGCACC CCACTCAGCA CTCACGCCCC GGCTCCCCCG 
GCAAGCTTTC CCACCAAGAA ACCTCCCAGC CCCGAGCCCA AGCACAGCGA GCTCCTGGTC 
AGTGTCGGCA GCATCACCTC CGACCACGCC GGCACACCGT CTGCTCCCTA CCTGGCCGGC 
CTCGACTCCA GCCGGCTGAG CCCCGAGGTG CAGTCCATGG CCGAGAGCCG GGGATTTGAA 
GCTGATGACG AGAGGAGCGA GCTGGTCAGC GAGGGCCGGC CAGTGGAGAC AGACAGCGAG 
AGCGACTTTC CGGTCTTCCC CGCCGCCCCG GCCTCGGACA GGCTCTTCCG AGGAAAACTC 
CAAGAGGCCG CGAGGACCAG CCGGAGAAAC TCAGAAGGCA GTGAGGTCAG CTGCACGGAA 
GGAAGTTTAA CACCAAGTTT AGAAAGCCGG AGACAGCTCT TCAGCTCCCA TAAACTGATC 
GAGTGTGACA CTCTGTCCAG GAAGAAATCC GCCAGGTTCA AGTCAGACAG TGGGAGTCTA 
GGAGATGCCA AGAATGAGAA AGAAGCCCCT TCCATCACCA AAGTGTTCGA CGTTATGAAA 
AAAGGAAAAT CCACCGGAAA TTTACTGACA CCACCAGCCA GAAGCGAATC GGACAAACAG 
GAACCCACTT GGAAAACAAA AATAGCCGAT CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC 
GATGACATGT TTGGAGTAGG AAGTCAAAAA ACCATCGCCG AACCTGCCAA AAGGAAAAAC 
ATCAAACGCA GACACACGCT GGGTGGGCAC AGGGATGCTA CTGAAATGAG TGTTCTGAAT 
TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC AGAGAATCCG AACTTTCAGC TGTGAATCGA 
TTGAAGCCCA AATGCTCCGC CCAGGACCTG TCCATCTCAG ACTGGCTGGC CAGGGAGCGG 
CTACGCACCA GTATGACTGA CCTGAGCAGA GGGGACACGG GGGACCCCCG GCCTGAGAGT 
CTTGGCACCT TAGAAATACC AACCAGGGAC CCGCCCCTGT CTTTCCAGTC TGATGCAGAC 
AGTTCTTCCA GCACCTTGGC TTCAACTAAC AGGGCCCCTC TTTCCACACC ATCACAGTCA 
CCTGACCAAA TAAATGGAGA AAGCTTCCAG AACACGAGCC AAAACTCCAG TTCTGCAGCC 
AGGGTCCAAC CTCATCAACT GTCTGAAACC CCAGATCACA AAGCACAGTT CCATCCCTGT 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_005194290.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005194347.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X4, mRNA.

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CloneID	OBa07465
Clone ID Related Accession (Same CDS sequence)	XM_005214301.3 , XM_005194290.3
Accession Version	XM_005194290.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646129.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194290.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AAAGCAGAGC CATTGCCGAA GACCCCTCGC 
CAGAGTCTCA GCATCCGGCA GACCCTACTT GGTGCTAAAG CAGAGCCTCG GACTCAGAGT 
CCACACTCTC CCAAAGAGGA GTCAGAAAGG AAGCTTCTTA GTAAAGATGA TACCAGTCCT 
CCAAAAGACA AAGGCACGTG GAGAAAAGGC ATTCCAAGTA TTATGAGAAA GACATTTGAG 
AAAAAGCCTA CTGCTACGGG AACATTCGGG GTCAGACTGG ACGACTGCCC ACCAGCTCAT 
ACAAACCGGT ATATTCCATT AATAGTTGAC ATATGTTGTA AATTAGTTGA AGAAAGAGGT 
CTGGAATATA CAGGTATTTA CAGAGTCCCT GGAAACAATG CAGCCATCTC CAGTATGCAG 
GAGGAGCTCA ACAAGGGAAT GGCTGATATT GATATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATCA GCAGTTTATT AAAATCCTTC TTCAGAAAAC TCCCAGAACC TCTCTTCACG 
AATGATAAAT ACGCCGACTT TATTGAAGCC AATCGTAAGG AAGATCCTCT AGATCGTCTG 
AAAACATTAA AAAGACTAAT TCATGATTTG CCCGAACATC ATTATGAAAC ACTCAAGTTT 
CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA AATTCAGAAA AAAATAAGAT GGAACCAAGA 
AACCTAGCCA TAGTGTTTGG TCCAACTCTT GTGAGAACCT CCGAAGATAA CATGACACAC 
ATGGTCACTC ACATGCCAGA TCAGTACAAG ATCGTAGAGA CACTCATCCA GCACCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA CCTCTTACAA CAGTGCAGGA GGAAAACACA 
GTAGACTCCC AGCCAGTGCC AAACATAGAT CATTTACTCA CCAACATTGG AAGGACAGGC 
GTCTCCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CAACAAAATC TAAGGGTTCT 
TGGGGATCCG GAAAAGACCA GTACAGCAAG GACCTGCTTG TATCCTCCAT CTTCGCGGCT 
GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA AAAGCACAAC CCAGCAGCTC GGAAGACGAG 
CTGGACAATG TGTTTTTCAA GAAAGAAAAC TCAGAGCAGG GTCACCACGA CATTAAAGAA 
GAGTCCAAAA AAGAGAGTGA GACACCAGGC CGGAAACAAA GGACCGTTGC TCCCAAAGAA 
AACAACACGA AGAAAGACAG CAGCGGAGGT GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG 
GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC ACCAAGCAGA GCCGGTCACC CACCCTGAGC 
TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC AGGGCCCTCG CAGCCCAGAA GACCTCCCAC 
CTGGAAGACA CGGGGTCCGA CTCCAGCACC CCACTCAGCA CTCACGCCCC GGCTCCCCCG 
GCAAGCTTTC CCACCAAGAA ACCTCCCAGC CCCGAGCCCA AGCACAGCGA GCTCCTGGTC 
AGTGTCGGCA GCATCACCTC CGACCACGCC GGCACACCGT CTGCTCCCTA CCTGGCCGGC 
CTCGACTCCA GCCGGCTGAG CCCCGAGGTG CAGTCCATGG CCGAGAGCCG GGGATTTGAA 
GCTGATGACG AGAGGAGCGA GCTGGTCAGC GAGGGCCGGC CAGTGGAGAC AGACAGCGAG 
AGCGACTTTC CGGTCTTCCC CGCCGCCCCG GCCTCGGACA GGCTCTTCCG AGGAAAACTC 
CAAGAGGCCG CGAGGACCAG CCGGAGAAAC TCAGAAGGCA GTGAGGTCAG CTGCACGGAA 
GGAAGTTTAA CACCAAGTTT AGAAAGCCGG AGACAGCTCT TCAGCTCCCA TAAACTGATC 
GAGTGTGACA CTCTGTCCAG GAAGAAATCC GCCAGGTTCA AGTCAGACAG TGGGAGTCTA 
GGAGATGCCA AGAATGAGAA AGAAGCCCCT TCCATCACCA AAGTGTTCGA CGTTATGAAA 
AAAGGAAAAT CCACCGGAAA TTTACTGACA CCACCAGCCA GAAGCGAATC GGACAAACAG 
GAACCCACTT GGAAAACAAA AATAGCCGAT CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC 
GATGACATGT TTGGAGTAGG AAGTCAAAAA ACCATCGCCG AACCTGCCAA AAGGAAAAAC 
ATCAAACGCA GACACACGCT GGGTGGGCAC AGGGATGCTA CTGAAATGAG TGTTCTGAAT 
TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC AGAGAATCCG AACTTTCAGC TGTGAATCGA 
TTGAAGCCCA AATGCTCCGC CCAGGACCTG TCCATCTCAG ACTGGCTGGC CAGGGAGCGG 
CTACGCACCA GTATGACTGA CCTGAGCAGA GGGGACACGG GGGACCCCCG GCCTGAGAGT 
CTTGGCACCT TAGAAATACC AACCAGGGAC CCGCCCCTGT CTTTCCAGTC TGATGCAGAC 
AGTTCTTCCA GCACCTTGGC TTCAACTAAC AGGGCCCCTC TTTCCACACC ATCACAGTCA 
CCTGACCAAA TAAATGGAGA AAGCTTCCAG AACACGAGCC AAAACTCCAG TTCTGCAGCC 
AGGGTCCAAC CTCATCAACT GTCTGAAACC CCAGATCACA AAGCACAGTT CCATCCCTGT 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005194347.1
CDS	539..6424
Translation	MMATRRTGLPEGDGDTKLKACGSPTCEVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_005194290.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005194290.3

ORF Insert Sequence:

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGCTACAAT TTACAAAGGA TGTCACAGCT 
CTGGCGTATT CTCAAGATGC CTACCTGAAA GGCAATGAAG CCTACAGCGG CAATGCCCGC 
AACATCCCTG AGCCCCCACC AGTCTGCTAC CCCTGGCCGT CATCCGCCCC TCCCGCCATG 
GCGCAGCCCC CTGAACCACC TCCTCCAGAC TCCACAGCAA GCAAACCGCA GACTAGCCCC 
CCAGTATTGA CGCAGCCCGG CAGGGCATAC AGGATGGAAA TCCAAGTGCC TCCGTCACCA 
CCAGAGGTGG CCAAGTCCAA CACAGCAGTG TGTGTCTGCA ATGAGAGTGT GAGGACCGTC 
ATTGTACCTT CTGAGAAGGT TGTAGATTTA TTACCTAACA GAAGCAACCA CACAGTTCCC 
GCTCACAGAA CTGAGGAAGT GAGGTATGGC CGAAACGAAC AGCCCTCTTT CAAAACAGCG 
ACGAGAACCA CCTCACCGCC ATCCTCAGTT CCCACTGCCC ATCTCATCCA CCAGCCCGGC 
TCCAGGTCGT TGGAACCTTC TGGCATTTTA CTTAAATCTG GCCACTACGG CGGACACTCA 
GAAGGTCTGG TGAGCAGATC TCCAGCTGTG GAGTCTCCTT CCGTAACTGT CAACCACTAC 
TCTCCCAACT CCCATCAGCA CATAGACTGG AAAAACTATA AAACTTACAA AGAGTATATT 
GATAACCGGC GCTTGCACAT GGGCTGTCGG ACCATTCAAG AAAGGTTAGA TAGTTTAAGA 
GCCGCCTCTC AGAGCACAGC GGATTATAAC CAGGTGGTGC TGAACCGCGC TGCCCTGCAG 
GTACGGCGTC GGAGCACCTC TCAGGACCGA GTGCCCCAGT CCATCCAGGT CCGGCAGCGC 
AGCGTGTCCC AGGAGAGGCT GGAGGACTCT GTGTTGATGA TGTATTGTCC GAGGAGTGCC 
TCTCAAGGTG CGCTGACATC CCCTTCTATT AGTTTTAGTA ACCACAGAAC TCGCTCGTGG 
GATTACATCG AGGGACAGGG TGAAACCTCG GAAAACGTCC ATTCTGAATG TCCACCGCCG 
GATGCAAATG GTGAACGAAA GCAGACTTAC AAGTGGAGTG GGTTCACCGA ACAGGACGAT 
CGTCGAGGTA TTTACGAGAG ACCCAGGCAG CAAGAAATTC ACAAATCTTT CCGAGGCTCC 
AATTTTACAG TGGCTCCCAG TGTGGTGAAT TCTGACAGCA GGAGAGTGAG CAGCAGAATC 
GGGGGATCAG TGTCTCAGTT TAAAAAAATT CCAGCAGATC TAAAACCCCT GCAGCCCAGC 
AGAAATTTTC AAACTGCTTG TGGAATATCC CAGCCTCGAG GGATTTCCCA AGACAGGTCA 
CCTCTTGGGA AAGTCCGAAG TAACTCTCTG AAAGGGCCTT CTATGCATGT CGCGAAACCG 
CCCTCCAGCC AGAACTCATT TCTTTCTATC AAAGACCAAA GACCAGTAAA TCACTTGCAT 
CAAAACAGTT TATTGAATCA GCAGCCATGG TTACGGACGG AAAGTGCCCC CGATCACCAA 
GTGGACACTG GGAAGCCCCC TTCTCTTTCC GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA 
AGTGAGAATG CTAGCACTCC AGATTTTGAG TTATCCGTCT CTCAGAGGAA TCAAGATTTA 
AATGTACAAG AGGTGGAAAT TCAGCAATCG AATGCTGTAG ATAATAAAGA AACTGTCATC 
CTAAGAGAAA AACCTCCGTC TGGTCGCCAG ACACCGCAGC CTTTAAGGCA TCAGTCTTAC 
ATCTTAGCAG TGAGTGACCA GGAGACAGGC TCAGACACCA CCTGCTGGCT GCCTAATGAT 
GCTCGCCGGG AGGTCCATAT AAAAAGGATG GAGGAGAGGA AGGCCTCAAG TACCACTCCA 
CCTGGTGATT CCTTGGCTTC CATCCCATTT ATAGATGAAC CTACCAGCCC TAGCATCGAT 
CACGACATTT CTCACATCCC CGCCTCTGCG GTCATCTCTG CCTCCACCTC CCAGGTACCC 
TCCATAGCAA CGGTCCCTCC CAGCCTCACA ACTTCAGCTC CATTAATCCG ACGTCAGCTC 
TCCCATGACC AGGAATCTGT TGGACCTCCC AGCCTGGATG CCCAGCCCAG CTCAAAGACA 
GAGCGATCCA AATCATATGA TGAGGGCCTG GATGATTACA GAGAAGATGC AAAACTGTCT 
TTTAAACATG TATCTAGCCT GAAGGGAATC AAGATCTTAG ACAGTCAAAA GTCATCAGAA 
GATTCCGGAT CCAGGAAAGA CTCTTCCTCA GAGGTTTTCA GCGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCAGGCCACT CGTCACTGAT AAGGGCAAGC GCGTTGGTGG AAGTATTCGG 
CCATGGAAGC AGATGTATGT TGTACTTCGG GGCCATTCGC TGTATCTGTA CAAAGATAAA 
CGAGAACAGA CGACTCCGTC TGAGGAGGAG CAGCCCATCA GTGTTAACGC CTGCTTAATA 
GACATCTCTT ACAGCGAGAC CAAGAGGAAG AATGTCTTTC GACTCACCAC GTCTGACTGC 
GAGTGCCTGT TTCAGGCTGA AGACAGGGAT GACATGTTAG CCTGGATCAA AACCATCCAG 
GAGAGCAGCA ATTTAAACGA GGAGGACACT GGAGTCACTA ACAGGGACCT AATTAGTCGA 
AGAATAAAAG AATACAACAG TCTAATGAGC AAAGCAGAGC CATTGCCGAA GACCCCTCGC 
CAGAGTCTCA GCATCCGGCA GACCCTACTT GGTGCTAAAG CAGAGCCTCG GACTCAGAGT 
CCACACTCTC CCAAAGAGGA GTCAGAAAGG AAGCTTCTTA GTAAAGATGA TACCAGTCCT 
CCAAAAGACA AAGGCACGTG GAGAAAAGGC ATTCCAAGTA TTATGAGAAA GACATTTGAG 
AAAAAGCCTA CTGCTACGGG AACATTCGGG GTCAGACTGG ACGACTGCCC ACCAGCTCAT 
ACAAACCGGT ATATTCCATT AATAGTTGAC ATATGTTGTA AATTAGTTGA AGAAAGAGGT 
CTGGAATATA CAGGTATTTA CAGAGTCCCT GGAAACAATG CAGCCATCTC CAGTATGCAG 
GAGGAGCTCA ACAAGGGAAT GGCTGATATT GATATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATCA GCAGTTTATT AAAATCCTTC TTCAGAAAAC TCCCAGAACC TCTCTTCACG 
AATGATAAAT ACGCCGACTT TATTGAAGCC AATCGTAAGG AAGATCCTCT AGATCGTCTG 
AAAACATTAA AAAGACTAAT TCATGATTTG CCCGAACATC ATTATGAAAC ACTCAAGTTT 
CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA AATTCAGAAA AAAATAAGAT GGAACCAAGA 
AACCTAGCCA TAGTGTTTGG TCCAACTCTT GTGAGAACCT CCGAAGATAA CATGACACAC 
ATGGTCACTC ACATGCCAGA TCAGTACAAG ATCGTAGAGA CACTCATCCA GCACCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA CCTCTTACAA CAGTGCAGGA GGAAAACACA 
GTAGACTCCC AGCCAGTGCC AAACATAGAT CATTTACTCA CCAACATTGG AAGGACAGGC 
GTCTCCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CAACAAAATC TAAGGGTTCT 
TGGGGATCCG GAAAAGACCA GTACAGCAAG GACCTGCTTG TATCCTCCAT CTTCGCGGCT 
GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA AAAGCACAAC CCAGCAGCTC GGAAGACGAG 
CTGGACAATG TGTTTTTCAA GAAAGAAAAC TCAGAGCAGG GTCACCACGA CATTAAAGAA 
GAGTCCAAAA AAGAGAGTGA GACACCAGGC CGGAAACAAA GGACCGTTGC TCCCAAAGAA 
AACAACACGA AGAAAGACAG CAGCGGAGGT GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG 
GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC ACCAAGCAGA GCCGGTCACC CACCCTGAGC 
TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC AGGGCCCTCG CAGCCCAGAA GACCTCCCAC 
CTGGAAGACA CGGGGTCCGA CTCCAGCACC CCACTCAGCA CTCACGCCCC GGCTCCCCCG 
GCAAGCTTTC CCACCAAGAA ACCTCCCAGC CCCGAGCCCA AGCACAGCGA GCTCCTGGTC 
AGTGTCGGCA GCATCACCTC CGACCACGCC GGCACACCGT CTGCTCCCTA CCTGGCCGGC 
CTCGACTCCA GCCGGCTGAG CCCCGAGGTG CAGTCCATGG CCGAGAGCCG GGGATTTGAA 
GCTGATGACG AGAGGAGCGA GCTGGTCAGC GAGGGCCGGC CAGTGGAGAC AGACAGCGAG 
AGCGACTTTC CGGTCTTCCC CGCCGCCCCG GCCTCGGACA GGCTCTTCCG AGGAAAACTC 
CAAGAGGCCG CGAGGACCAG CCGGAGAAAC TCAGAAGGCA GTGAGGTCAG CTGCACGGAA 
GGAAGTTTAA CACCAAGTTT AGAAAGCCGG AGACAGCTCT TCAGCTCCCA TAAACTGATC 
GAGTGTGACA CTCTGTCCAG GAAGAAATCC GCCAGGTTCA AGTCAGACAG TGGGAGTCTA 
GGAGATGCCA AGAATGAGAA AGAAGCCCCT TCCATCACCA AAGTGTTCGA CGTTATGAAA 
AAAGGAAAAT CCACCGGAAA TTTACTGACA CCACCAGCCA GAAGCGAATC GGACAAACAG 
GAACCCACTT GGAAAACAAA AATAGCCGAT CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC 
GATGACATGT TTGGAGTAGG AAGTCAAAAA ACCATCGCCG AACCTGCCAA AAGGAAAAAC 
ATCAAACGCA GACACACGCT GGGTGGGCAC AGGGATGCTA CTGAAATGAG TGTTCTGAAT 
TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC AGAGAATCCG AACTTTCAGC TGTGAATCGA 
TTGAAGCCCA AATGCTCCGC CCAGGACCTG TCCATCTCAG ACTGGCTGGC CAGGGAGCGG 
CTACGCACCA GTATGACTGA CCTGAGCAGA GGGGACACGG GGGACCCCCG GCCTGAGAGT 
CTTGGCACCT TAGAAATACC AACCAGGGAC CCGCCCCTGT CTTTCCAGTC TGATGCAGAC 
AGTTCTTCCA GCACCTTGGC TTCAACTAAC AGGGCCCCTC TTTCCACACC ATCACAGTCA 
CCTGACCAAA TAAATGGAGA AAGCTTCCAG AACACGAGCC AAAACTCCAG TTCTGCAGCC 
AGGGTCCAAC CTCATCAACT GTCTGAAACC CCAGATCACA AAGCACAGTT CCATCCCTGT 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_025000963.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856731.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X9, mRNA.

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CloneID	OBa07466
Clone ID Related Accession (Same CDS sequence)	XM_025000963.1 , XM_005194291.3 , XM_005214302.3
Accession Version	XM_025000963.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGTG CTGAATGGAG ATGCACAGAT GCTGTTATCA AATGCAGCTA TTTTCTGTTA 
CAGAAAGACC ATATGGTATC AAAAAGTAAA GATGGAAAAG AACAAAGTGA AACTGTATCA 
CCATCCGAAG ATGAAATGTT CTCCTGGCCA GGTCCCAAAA CAGTTATGTT GAAAAGAACA 
TCTCAAGGCT TTGGTTTTAC ATTGAGGCAT TTTATTGTTT ATCCCCCAGA GTCTGCAATT 
CAGTTTTCAT TTAAGGATGA AGAAAATGGC AACAGAGGAG GAAAACAAAG AAACCGTTTG 
GAACCCATGG ACACCATCTT TGTTAAACAG GTGAAAGAGG GCGGACCTGC TTTTGAAGCT 
GGATTATGCA CAGGTGATCG AATTATAAAA GTCAATGGAG AAAGTGTTAT TGGAAAGACC 
TATTCTCAAG TAATTGCTTT AATCCAGAAC AGTGATACAA CATTGGAACT TAGTGTTATG 
CCAAAAGATG AAGACATTCT CCAAGTGCTA CAATTTACAA AGGATGTCAC AGCTCTGGCG 
TATTCTCAAG ATGCCTACCT GAAAGGCAAT GAAGCCTACA GCGGCAATGC CCGCAACATC 
CCTGAGCCCC CACCAGTCTG CTACCCCTGG CCGTCATCCG CCCCTCCCGC CATGGCGCAG 
CCCCCTGAAC CACCTCCTCC AGACTCCACA GCAAGCAAAC CGCAGACTAG CCCCCCAGTA 
TTGACGCAGC CCGGCAGGGC ATACAGGATG GAAATCCAAG TGCCTCCGTC ACCACCAGAG 
GTGGCCAAGT CCAACACAGC AGTGTGTGTC TGCAATGAGA GTGTGAGGAC CGTCATTGTA 
CCTTCTGAGA AGGTTGTAGA TTTATTACCT AACAGAAGCA ACCACACAGT TCCCGCTCAC 
AGAACTGAGG AAGTGAGGTA TGGCCGAAAC GAACAGCCCT CTTTCAAAAC AGCGACGAGA 
ACCACCTCAC CGCCATCCTC AGTTCCCACT GCCCATCTCA TCCACCAGCC CGGCTCCAGG 
TCGTTGGAAC CTTCTGGCAT TTTACTTAAA TCTGGCCACT ACGGCGGACA CTCAGAAGGT 
CTGGTGAGCA GATCTCCAGC TGTGGAGTCT CCTTCCGTAA CTGTCAACCA CTACTCTCCC 
AACTCCCATC AGCACATAGA CTGGAAAAAC TATAAAACTT ACAAAGAGTA TATTGATAAC 
CGGCGCTTGC ACATGGGCTG TCGGACCATT CAAGAAAGGT TAGATAGTTT AAGAGCCGCC 
TCTCAGAGCA CAGCGGATTA TAACCAGGTG GTGCTGAACC GCGCTGCCCT GCAGGTACGG 
CGTCGGAGCA CCTCTCAGGA CCGAGTGCCC CAGTCCATCC AGGTCCGGCA GCGCAGCGTG 
TCCCAGGAGA GGCTGGAGGA CTCTGTGTTG ATGATGTATT GTCCGAGGAG TGCCTCTCAA 
GGTGCGCTGA CATCCCCTTC TATTAGTTTT AGTAACCACA GAACTCGCTC GTGGGATTAC 
ATCGAGGGAC AGGGTGAAAC CTCGGAAAAC GTCCATTCTG AATGTCCACC GCCGGATGCA 
AATGGTGAAC GAAAGCAGAC TTACAAGTGG AGTGGGTTCA CCGAACAGGA CGATCGTCGA 
GGTATTTACG AGAGACCCAG GCAGCAAGAA ATTCACAAAT CTTTCCGAGG CTCCAATTTT 
ACAGTGGCTC CCAGTGTGGT GAATTCTGAC AGCAGGAGAG TGAGCAGCAG AATCGGGGGA 
TCAGTGTCTC AGTTTAAAAA AATTCCAGCA GATCTAAAAC CCCTGCAGCC CAGCAGAAAT 
TTTCAAACTG CTTGTGGAAT ATCCCAGCCT CGAGGGATTT CCCAAGACAG GTCACCTCTT 
GGGAAAGTCC GAAGTAACTC TCTGAAAGGG CCTTCTATGC ATGTCGCGAA ACCGCCCTCC 
AGCCAGAACT CATTTCTTTC TATCAAAGAC CAAAGACCAG TAAATCACTT GCATCAAAAC 
AGTTTATTGA ATCAGCAGCC ATGGTTACGG ACGGAAAGTG CCCCCGATCA CCAAGTGGAC 
ACTGGGAAGC CCCCTTCTCT TTCCGGAGCT TCTGTTAAGC CCCCGGCTCT GGCAAGTGAG 
AATGCTAGCA CTCCAGATTT TGAGTTATCC GTCTCTCAGA GGAATCAAGA TTTAAATGTA 
CAAGAGGTGG AAATTCAGCA ATCGAATGCT GTAGATAATA AAGAAACTGT CATCCTAAGA 
GAAAAACCTC CGTCTGGTCG CCAGACACCG CAGCCTTTAA GGCATCAGTC TTACATCTTA 
GCAGTGAGTG ACCAGGAGAC AGGCTCAGAC ACCACCTGCT GGCTGCCTAA TGATGCTCGC 
CGGGAGGTCC ATATAAAAAG GATGGAGGAG AGGAAGGCCT CAAGTACCAC TCCACCTGGT 
GATTCCTTGG CTTCCATCCC ATTTATAGAT GAACCTACCA GCCCTAGCAT CGATCACGAC 
ATTTCTCACA TCCCCGCCTC TGCGGTCATC TCTGCCTCCA CCTCCCAGGT ACCCTCCATA 
GCAACGGTCC CTCCCAGCCT CACAACTTCA GCTCCATTAA TCCGACGTCA GCTCTCCCAT 
GACCAGGAAT CTGTTGGACC TCCCAGCCTG GATGCCCAGC CCAGCTCAAA GACAGAGCGA 
TCCAAATCAT ATGATGAGGG CCTGGATGAT TACAGAGAAG ATGCAAAACT GTCTTTTAAA 
CATGTATCTA GCCTGAAGGG AATCAAGATC TTAGACAGTC AAAAGTCATC AGAAGATTCC 
GGATCCAGGA AAGACTCTTC CTCAGAGGTT TTCAGCGATG CTGCCAAGGA AGGGTGGCTT 
CATTTCAGGC CACTCGTCAC TGATAAGGGC AAGCGCGTTG GTGGAAGTAT TCGGCCATGG 
AAGCAGATGT ATGTTGTACT TCGGGGCCAT TCGCTGTATC TGTACAAAGA TAAACGAGAA 
CAGACGACTC CGTCTGAGGA GGAGCAGCCC ATCAGTGTTA ACGCCTGCTT AATAGACATC 
TCTTACAGCG AGACCAAGAG GAAGAATGTC TTTCGACTCA CCACGTCTGA CTGCGAGTGC 
CTGTTTCAGG CTGAAGACAG GGATGACATG TTAGCCTGGA TCAAAACCAT CCAGGAGAGC 
AGCAATTTAA ACGAGGAGGA CACTGGAGTC ACTAACAGGG ACCTAATTAG TCGAAGAATA 
AAAGAATACA ACAGTCTAAT GAGCAGCAAA GCAGAGCCAT TGCCGAAGAC CCCTCGCCAG 
AGTCTCAGCA TCCGGCAGAC CCTACTTGGT GCTAAAGCAG AGCCTCGGAC TCAGAGTCCA 
CACTCTCCCA AAGAGGAGTC AGAAAGGAAG CTTCTTAGTA AAGATGATAC CAGTCCTCCA 
AAAGACAAAG GCACGTGGAG AAAAGGCATT CCAAGTATTA TGAGAAAGAC ATTTGAGAAA 
AAGCCTACTG CTACGGGAAC ATTCGGGGTC AGACTGGACG ACTGCCCACC AGCTCATACA 
AACCGGTATA TTCCATTAAT AGTTGACATA TGTTGTAAAT TAGTTGAAGA AAGAGGTCTG 
GAATATACAG GTATTTACAG AGTCCCTGGA AACAATGCAG CCATCTCCAG TATGCAGGAG 
GAGCTCAACA AGGGAATGGC TGATATTGAT ATACAAGATG ATAAATGGCG AGATTTGAAT 
GTGATCAGCA GTTTATTAAA ATCCTTCTTC AGAAAACTCC CAGAACCTCT CTTCACGAAT 
GATAAATACG CCGACTTTAT TGAAGCCAAT CGTAAGGAAG ATCCTCTAGA TCGTCTGAAA 
ACATTAAAAA GACTAATTCA TGATTTGCCC GAACATCATT ATGAAACACT CAAGTTTCTT 
TCGGCTCATC TGAAGACAGT AGCAGAAAAT TCAGAAAAAA ATAAGATGGA ACCAAGAAAC 
CTAGCCATAG TGTTTGGTCC AACTCTTGTG AGAACCTCCG AAGATAACAT GACACACATG 
GTCACTCACA TGCCAGATCA GTACAAGATC GTAGAGACAC TCATCCAGCA CCATGACTGG 
TTTTTCACAG AAGAAGGTGC TGAAGAACCT CTTACAACAG TGCAGGAGGA AAACACAGTA 
GACTCCCAGC CAGTGCCAAA CATAGATCAT TTACTCACCA ACATTGGAAG GACAGGCGTC 
TCCCCTGGAG ATGTATCAGA TTCAGCTACT AGTGACTCAA CAAAATCTAA GGGTTCTTGG 
GGATCCGGAA AAGACCAGTA CAGCAAGGAC CTGCTTGTAT CCTCCATCTT CGCGGCTGCC 
AGTCGCAAGA GGAAAAAGCC AAAAGAAAAA GCACAACCCA GCAGCTCGGA AGACGAGCTG 
GACAATGTGT TTTTCAAGAA AGAAAACTCA GAGCAGGGTC ACCACGACAT TAAAGAAGAG 
TCCAAAAAAG AGAGTGAGAC ACCAGGCCGG AAACAAAGGA CCGTTGCTCC CAAAGAAAAC 
AACACGAAGA AAGACAGCAG CGGAGGTGGC AGAGCTGAGC AGAGGGCCCC GCGTGGGGAG 
AGCTTGGAGC CCCCGGCCCC CCAAAGCACC AAGCAGAGCC GGTCACCCAC CCTGAGCTGT 
CGCCTCGCCG TCCTGAGAGA GAGCCCCAGG GCCCTCGCAG CCCAGAAGAC CTCCCACCTG 
GAAGACACGG GGTCCGACTC CAGCACCCCA CTCAGCACTC ACGCCCCGGC TCCCCCGGCA 
AGCTTTCCCA CCAAGAAACC TCCCAGCCCC GAGCCCAAGC ACAGCGAGCT CCTGGTCAGT 
GTCGGCAGCA TCACCTCCGA CCACGCCGGC ACACCGTCTG CTCCCTACCT GGCCGGCCTC 
GACTCCAGCC GGCTGAGCCC CGAGGTGCAG TCCATGGCCG AGAGCCGGGG ATTTGAAGCT 
GATGACGAGA GGAGCGAGCT GGTCAGCGAG GGCCGGCCAG TGGAGACAGA CAGCGAGAGC 
GACTTTCCGG TCTTCCCCGC CGCCCCGGCC TCGGACAGGC TCTTCCGAGG AAAACTCCAA 
GAGGCCGCGA GGACCAGCCG GAGAAACTCA GAAGGCAGTG AGGTCAGCTG CACGGAAGGA 
AGTTTAACAC CAAGTTTAGA AAGCCGGAGA CAGCTCTTCA GCTCCCATAA ACTGATCGAG 
TGTGACACTC TGTCCAGGAA GAAATCCGCC AGGTTCAAGT CAGACAGTGG GAGTCTAGGA 
GATGCCAAGA ATGAGAAAGA AGCCCCTTCC ATCACCAAAG TGTTCGACGT TATGAAAAAA 
GGAAAATCCA CCGGAAATTT ACTGACACCA CCAGCCAGAA GCGAATCGGA CAAACAGGAA 
CCCACTTGGA AAACAAAAAT AGCCGATCGG TTAAAACTGA GGCCCAAAGC CCCGGCCGAT 
GACATGTTTG GAGTAGGAAG TCAAAAAACC ATCGCCGAAC CTGCCAAAAG GAAAAACATC 
AAACGCAGAC ACACGCTGGG TGGGCACAGG GATGCTACTG AAATGAGTGT TCTGAATTTC 
TGGAAAGCCC AGGAGCACAG CGGGGACAGA GAATCCGAAC TTTCAGCTGT GAATCGATTG 
AAGCCCAAAT GCTCCGCCCA GGACCTGTCC ATCTCAGACT GGCTGGCCAG GGAGCGGCTA 
CGCACCAGTA TGACTGACCT GAGCAGAGGG GACACGGGGG ACCCCCGGCC TGAGAGTCTT 
GGCACCTTAG AAATACCAAC CAGGGACCCG CCCCTGTCTT TCCAGTCTGA TGCAGACAGT 
TCTTCCAGCA CCTTGGCTTC AACTAACAGG GCCCCTCTTT CCACACCATC ACAGTCACCT 
GACCAAATAA ATGGAGAAAG CTTCCAGAAC ACGAGCCAAA ACTCCAGTTC TGCAGCCAGG 
GTCCAACCTC ATCAACTGTC TGAAACCCCA GATCACAAAG CACAGTTCCA TCCCTGTCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856731.1
CDS	646..6528
Translation	MGRAEWRCTDAVIKCSYFLLQKDHMVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_025000963.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000963.1

ORF Insert Sequence:

ATGGGCCGTG CTGAATGGAG ATGCACAGAT GCTGTTATCA AATGCAGCTA TTTTCTGTTA 
CAGAAAGACC ATATGGTATC AAAAAGTAAA GATGGAAAAG AACAAAGTGA AACTGTATCA 
CCATCCGAAG ATGAAATGTT CTCCTGGCCA GGTCCCAAAA CAGTTATGTT GAAAAGAACA 
TCTCAAGGCT TTGGTTTTAC ATTGAGGCAT TTTATTGTTT ATCCCCCAGA GTCTGCAATT 
CAGTTTTCAT TTAAGGATGA AGAAAATGGC AACAGAGGAG GAAAACAAAG AAACCGTTTG 
GAACCCATGG ACACCATCTT TGTTAAACAG GTGAAAGAGG GCGGACCTGC TTTTGAAGCT 
GGATTATGCA CAGGTGATCG AATTATAAAA GTCAATGGAG AAAGTGTTAT TGGAAAGACC 
TATTCTCAAG TAATTGCTTT AATCCAGAAC AGTGATACAA CATTGGAACT TAGTGTTATG 
CCAAAAGATG AAGACATTCT CCAAGTGCTA CAATTTACAA AGGATGTCAC AGCTCTGGCG 
TATTCTCAAG ATGCCTACCT GAAAGGCAAT GAAGCCTACA GCGGCAATGC CCGCAACATC 
CCTGAGCCCC CACCAGTCTG CTACCCCTGG CCGTCATCCG CCCCTCCCGC CATGGCGCAG 
CCCCCTGAAC CACCTCCTCC AGACTCCACA GCAAGCAAAC CGCAGACTAG CCCCCCAGTA 
TTGACGCAGC CCGGCAGGGC ATACAGGATG GAAATCCAAG TGCCTCCGTC ACCACCAGAG 
GTGGCCAAGT CCAACACAGC AGTGTGTGTC TGCAATGAGA GTGTGAGGAC CGTCATTGTA 
CCTTCTGAGA AGGTTGTAGA TTTATTACCT AACAGAAGCA ACCACACAGT TCCCGCTCAC 
AGAACTGAGG AAGTGAGGTA TGGCCGAAAC GAACAGCCCT CTTTCAAAAC AGCGACGAGA 
ACCACCTCAC CGCCATCCTC AGTTCCCACT GCCCATCTCA TCCACCAGCC CGGCTCCAGG 
TCGTTGGAAC CTTCTGGCAT TTTACTTAAA TCTGGCCACT ACGGCGGACA CTCAGAAGGT 
CTGGTGAGCA GATCTCCAGC TGTGGAGTCT CCTTCCGTAA CTGTCAACCA CTACTCTCCC 
AACTCCCATC AGCACATAGA CTGGAAAAAC TATAAAACTT ACAAAGAGTA TATTGATAAC 
CGGCGCTTGC ACATGGGCTG TCGGACCATT CAAGAAAGGT TAGATAGTTT AAGAGCCGCC 
TCTCAGAGCA CAGCGGATTA TAACCAGGTG GTGCTGAACC GCGCTGCCCT GCAGGTACGG 
CGTCGGAGCA CCTCTCAGGA CCGAGTGCCC CAGTCCATCC AGGTCCGGCA GCGCAGCGTG 
TCCCAGGAGA GGCTGGAGGA CTCTGTGTTG ATGATGTATT GTCCGAGGAG TGCCTCTCAA 
GGTGCGCTGA CATCCCCTTC TATTAGTTTT AGTAACCACA GAACTCGCTC GTGGGATTAC 
ATCGAGGGAC AGGGTGAAAC CTCGGAAAAC GTCCATTCTG AATGTCCACC GCCGGATGCA 
AATGGTGAAC GAAAGCAGAC TTACAAGTGG AGTGGGTTCA CCGAACAGGA CGATCGTCGA 
GGTATTTACG AGAGACCCAG GCAGCAAGAA ATTCACAAAT CTTTCCGAGG CTCCAATTTT 
ACAGTGGCTC CCAGTGTGGT GAATTCTGAC AGCAGGAGAG TGAGCAGCAG AATCGGGGGA 
TCAGTGTCTC AGTTTAAAAA AATTCCAGCA GATCTAAAAC CCCTGCAGCC CAGCAGAAAT 
TTTCAAACTG CTTGTGGAAT ATCCCAGCCT CGAGGGATTT CCCAAGACAG GTCACCTCTT 
GGGAAAGTCC GAAGTAACTC TCTGAAAGGG CCTTCTATGC ATGTCGCGAA ACCGCCCTCC 
AGCCAGAACT CATTTCTTTC TATCAAAGAC CAAAGACCAG TAAATCACTT GCATCAAAAC 
AGTTTATTGA ATCAGCAGCC ATGGTTACGG ACGGAAAGTG CCCCCGATCA CCAAGTGGAC 
ACTGGGAAGC CCCCTTCTCT TTCCGGAGCT TCTGTTAAGC CCCCGGCTCT GGCAAGTGAG 
AATGCTAGCA CTCCAGATTT TGAGTTATCC GTCTCTCAGA GGAATCAAGA TTTAAATGTA 
CAAGAGGTGG AAATTCAGCA ATCGAATGCT GTAGATAATA AAGAAACTGT CATCCTAAGA 
GAAAAACCTC CGTCTGGTCG CCAGACACCG CAGCCTTTAA GGCATCAGTC TTACATCTTA 
GCAGTGAGTG ACCAGGAGAC AGGCTCAGAC ACCACCTGCT GGCTGCCTAA TGATGCTCGC 
CGGGAGGTCC ATATAAAAAG GATGGAGGAG AGGAAGGCCT CAAGTACCAC TCCACCTGGT 
GATTCCTTGG CTTCCATCCC ATTTATAGAT GAACCTACCA GCCCTAGCAT CGATCACGAC 
ATTTCTCACA TCCCCGCCTC TGCGGTCATC TCTGCCTCCA CCTCCCAGGT ACCCTCCATA 
GCAACGGTCC CTCCCAGCCT CACAACTTCA GCTCCATTAA TCCGACGTCA GCTCTCCCAT 
GACCAGGAAT CTGTTGGACC TCCCAGCCTG GATGCCCAGC CCAGCTCAAA GACAGAGCGA 
TCCAAATCAT ATGATGAGGG CCTGGATGAT TACAGAGAAG ATGCAAAACT GTCTTTTAAA 
CATGTATCTA GCCTGAAGGG AATCAAGATC TTAGACAGTC AAAAGTCATC AGAAGATTCC 
GGATCCAGGA AAGACTCTTC CTCAGAGGTT TTCAGCGATG CTGCCAAGGA AGGGTGGCTT 
CATTTCAGGC CACTCGTCAC TGATAAGGGC AAGCGCGTTG GTGGAAGTAT TCGGCCATGG 
AAGCAGATGT ATGTTGTACT TCGGGGCCAT TCGCTGTATC TGTACAAAGA TAAACGAGAA 
CAGACGACTC CGTCTGAGGA GGAGCAGCCC ATCAGTGTTA ACGCCTGCTT AATAGACATC 
TCTTACAGCG AGACCAAGAG GAAGAATGTC TTTCGACTCA CCACGTCTGA CTGCGAGTGC 
CTGTTTCAGG CTGAAGACAG GGATGACATG TTAGCCTGGA TCAAAACCAT CCAGGAGAGC 
AGCAATTTAA ACGAGGAGGA CACTGGAGTC ACTAACAGGG ACCTAATTAG TCGAAGAATA 
AAAGAATACA ACAGTCTAAT GAGCAGCAAA GCAGAGCCAT TGCCGAAGAC CCCTCGCCAG 
AGTCTCAGCA TCCGGCAGAC CCTACTTGGT GCTAAAGCAG AGCCTCGGAC TCAGAGTCCA 
CACTCTCCCA AAGAGGAGTC AGAAAGGAAG CTTCTTAGTA AAGATGATAC CAGTCCTCCA 
AAAGACAAAG GCACGTGGAG AAAAGGCATT CCAAGTATTA TGAGAAAGAC ATTTGAGAAA 
AAGCCTACTG CTACGGGAAC ATTCGGGGTC AGACTGGACG ACTGCCCACC AGCTCATACA 
AACCGGTATA TTCCATTAAT AGTTGACATA TGTTGTAAAT TAGTTGAAGA AAGAGGTCTG 
GAATATACAG GTATTTACAG AGTCCCTGGA AACAATGCAG CCATCTCCAG TATGCAGGAG 
GAGCTCAACA AGGGAATGGC TGATATTGAT ATACAAGATG ATAAATGGCG AGATTTGAAT 
GTGATCAGCA GTTTATTAAA ATCCTTCTTC AGAAAACTCC CAGAACCTCT CTTCACGAAT 
GATAAATACG CCGACTTTAT TGAAGCCAAT CGTAAGGAAG ATCCTCTAGA TCGTCTGAAA 
ACATTAAAAA GACTAATTCA TGATTTGCCC GAACATCATT ATGAAACACT CAAGTTTCTT 
TCGGCTCATC TGAAGACAGT AGCAGAAAAT TCAGAAAAAA ATAAGATGGA ACCAAGAAAC 
CTAGCCATAG TGTTTGGTCC AACTCTTGTG AGAACCTCCG AAGATAACAT GACACACATG 
GTCACTCACA TGCCAGATCA GTACAAGATC GTAGAGACAC TCATCCAGCA CCATGACTGG 
TTTTTCACAG AAGAAGGTGC TGAAGAACCT CTTACAACAG TGCAGGAGGA AAACACAGTA 
GACTCCCAGC CAGTGCCAAA CATAGATCAT TTACTCACCA ACATTGGAAG GACAGGCGTC 
TCCCCTGGAG ATGTATCAGA TTCAGCTACT AGTGACTCAA CAAAATCTAA GGGTTCTTGG 
GGATCCGGAA AAGACCAGTA CAGCAAGGAC CTGCTTGTAT CCTCCATCTT CGCGGCTGCC 
AGTCGCAAGA GGAAAAAGCC AAAAGAAAAA GCACAACCCA GCAGCTCGGA AGACGAGCTG 
GACAATGTGT TTTTCAAGAA AGAAAACTCA GAGCAGGGTC ACCACGACAT TAAAGAAGAG 
TCCAAAAAAG AGAGTGAGAC ACCAGGCCGG AAACAAAGGA CCGTTGCTCC CAAAGAAAAC 
AACACGAAGA AAGACAGCAG CGGAGGTGGC AGAGCTGAGC AGAGGGCCCC GCGTGGGGAG 
AGCTTGGAGC CCCCGGCCCC CCAAAGCACC AAGCAGAGCC GGTCACCCAC CCTGAGCTGT 
CGCCTCGCCG TCCTGAGAGA GAGCCCCAGG GCCCTCGCAG CCCAGAAGAC CTCCCACCTG 
GAAGACACGG GGTCCGACTC CAGCACCCCA CTCAGCACTC ACGCCCCGGC TCCCCCGGCA 
AGCTTTCCCA CCAAGAAACC TCCCAGCCCC GAGCCCAAGC ACAGCGAGCT CCTGGTCAGT 
GTCGGCAGCA TCACCTCCGA CCACGCCGGC ACACCGTCTG CTCCCTACCT GGCCGGCCTC 
GACTCCAGCC GGCTGAGCCC CGAGGTGCAG TCCATGGCCG AGAGCCGGGG ATTTGAAGCT 
GATGACGAGA GGAGCGAGCT GGTCAGCGAG GGCCGGCCAG TGGAGACAGA CAGCGAGAGC 
GACTTTCCGG TCTTCCCCGC CGCCCCGGCC TCGGACAGGC TCTTCCGAGG AAAACTCCAA 
GAGGCCGCGA GGACCAGCCG GAGAAACTCA GAAGGCAGTG AGGTCAGCTG CACGGAAGGA 
AGTTTAACAC CAAGTTTAGA AAGCCGGAGA CAGCTCTTCA GCTCCCATAA ACTGATCGAG 
TGTGACACTC TGTCCAGGAA GAAATCCGCC AGGTTCAAGT CAGACAGTGG GAGTCTAGGA 
GATGCCAAGA ATGAGAAAGA AGCCCCTTCC ATCACCAAAG TGTTCGACGT TATGAAAAAA 
GGAAAATCCA CCGGAAATTT ACTGACACCA CCAGCCAGAA GCGAATCGGA CAAACAGGAA 
CCCACTTGGA AAACAAAAAT AGCCGATCGG TTAAAACTGA GGCCCAAAGC CCCGGCCGAT 
GACATGTTTG GAGTAGGAAG TCAAAAAACC ATCGCCGAAC CTGCCAAAAG GAAAAACATC 
AAACGCAGAC ACACGCTGGG TGGGCACAGG GATGCTACTG AAATGAGTGT TCTGAATTTC 
TGGAAAGCCC AGGAGCACAG CGGGGACAGA GAATCCGAAC TTTCAGCTGT GAATCGATTG 
AAGCCCAAAT GCTCCGCCCA GGACCTGTCC ATCTCAGACT GGCTGGCCAG GGAGCGGCTA 
CGCACCAGTA TGACTGACCT GAGCAGAGGG GACACGGGGG ACCCCCGGCC TGAGAGTCTT 
GGCACCTTAG AAATACCAAC CAGGGACCCG CCCCTGTCTT TCCAGTCTGA TGCAGACAGT 
TCTTCCAGCA CCTTGGCTTC AACTAACAGG GCCCCTCTTT CCACACCATC ACAGTCACCT 
GACCAAATAA ATGGAGAAAG CTTCCAGAAC ACGAGCCAAA ACTCCAGTTC TGCAGCCAGG 
GTCCAACCTC ATCAACTGTC TGAAACCCCA GATCACAAAG CACAGTTCCA TCCCTGTCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_005194291.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005194348.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.

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CloneID	OBa07466
Clone ID Related Accession (Same CDS sequence)	XM_025000963.1 , XM_005194291.3 , XM_005214302.3
Accession Version	XM_005194291.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646129.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194291.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGTG CTGAATGGAG ATGCACAGAT GCTGTTATCA AATGCAGCTA TTTTCTGTTA 
CAGAAAGACC ATATGGTATC AAAAAGTAAA GATGGAAAAG AACAAAGTGA AACTGTATCA 
CCATCCGAAG ATGAAATGTT CTCCTGGCCA GGTCCCAAAA CAGTTATGTT GAAAAGAACA 
TCTCAAGGCT TTGGTTTTAC ATTGAGGCAT TTTATTGTTT ATCCCCCAGA GTCTGCAATT 
CAGTTTTCAT TTAAGGATGA AGAAAATGGC AACAGAGGAG GAAAACAAAG AAACCGTTTG 
GAACCCATGG ACACCATCTT TGTTAAACAG GTGAAAGAGG GCGGACCTGC TTTTGAAGCT 
GGATTATGCA CAGGTGATCG AATTATAAAA GTCAATGGAG AAAGTGTTAT TGGAAAGACC 
TATTCTCAAG TAATTGCTTT AATCCAGAAC AGTGATACAA CATTGGAACT TAGTGTTATG 
CCAAAAGATG AAGACATTCT CCAAGTGCTA CAATTTACAA AGGATGTCAC AGCTCTGGCG 
TATTCTCAAG ATGCCTACCT GAAAGGCAAT GAAGCCTACA GCGGCAATGC CCGCAACATC 
CCTGAGCCCC CACCAGTCTG CTACCCCTGG CCGTCATCCG CCCCTCCCGC CATGGCGCAG 
CCCCCTGAAC CACCTCCTCC AGACTCCACA GCAAGCAAAC CGCAGACTAG CCCCCCAGTA 
TTGACGCAGC CCGGCAGGGC ATACAGGATG GAAATCCAAG TGCCTCCGTC ACCACCAGAG 
GTGGCCAAGT CCAACACAGC AGTGTGTGTC TGCAATGAGA GTGTGAGGAC CGTCATTGTA 
CCTTCTGAGA AGGTTGTAGA TTTATTACCT AACAGAAGCA ACCACACAGT TCCCGCTCAC 
AGAACTGAGG AAGTGAGGTA TGGCCGAAAC GAACAGCCCT CTTTCAAAAC AGCGACGAGA 
ACCACCTCAC CGCCATCCTC AGTTCCCACT GCCCATCTCA TCCACCAGCC CGGCTCCAGG 
TCGTTGGAAC CTTCTGGCAT TTTACTTAAA TCTGGCCACT ACGGCGGACA CTCAGAAGGT 
CTGGTGAGCA GATCTCCAGC TGTGGAGTCT CCTTCCGTAA CTGTCAACCA CTACTCTCCC 
AACTCCCATC AGCACATAGA CTGGAAAAAC TATAAAACTT ACAAAGAGTA TATTGATAAC 
CGGCGCTTGC ACATGGGCTG TCGGACCATT CAAGAAAGGT TAGATAGTTT AAGAGCCGCC 
TCTCAGAGCA CAGCGGATTA TAACCAGGTG GTGCTGAACC GCGCTGCCCT GCAGGTACGG 
CGTCGGAGCA CCTCTCAGGA CCGAGTGCCC CAGTCCATCC AGGTCCGGCA GCGCAGCGTG 
TCCCAGGAGA GGCTGGAGGA CTCTGTGTTG ATGATGTATT GTCCGAGGAG TGCCTCTCAA 
GGTGCGCTGA CATCCCCTTC TATTAGTTTT AGTAACCACA GAACTCGCTC GTGGGATTAC 
ATCGAGGGAC AGGGTGAAAC CTCGGAAAAC GTCCATTCTG AATGTCCACC GCCGGATGCA 
AATGGTGAAC GAAAGCAGAC TTACAAGTGG AGTGGGTTCA CCGAACAGGA CGATCGTCGA 
GGTATTTACG AGAGACCCAG GCAGCAAGAA ATTCACAAAT CTTTCCGAGG CTCCAATTTT 
ACAGTGGCTC CCAGTGTGGT GAATTCTGAC AGCAGGAGAG TGAGCAGCAG AATCGGGGGA 
TCAGTGTCTC AGTTTAAAAA AATTCCAGCA GATCTAAAAC CCCTGCAGCC CAGCAGAAAT 
TTTCAAACTG CTTGTGGAAT ATCCCAGCCT CGAGGGATTT CCCAAGACAG GTCACCTCTT 
GGGAAAGTCC GAAGTAACTC TCTGAAAGGG CCTTCTATGC ATGTCGCGAA ACCGCCCTCC 
AGCCAGAACT CATTTCTTTC TATCAAAGAC CAAAGACCAG TAAATCACTT GCATCAAAAC 
AGTTTATTGA ATCAGCAGCC ATGGTTACGG ACGGAAAGTG CCCCCGATCA CCAAGTGGAC 
ACTGGGAAGC CCCCTTCTCT TTCCGGAGCT TCTGTTAAGC CCCCGGCTCT GGCAAGTGAG 
AATGCTAGCA CTCCAGATTT TGAGTTATCC GTCTCTCAGA GGAATCAAGA TTTAAATGTA 
CAAGAGGTGG AAATTCAGCA ATCGAATGCT GTAGATAATA AAGAAACTGT CATCCTAAGA 
GAAAAACCTC CGTCTGGTCG CCAGACACCG CAGCCTTTAA GGCATCAGTC TTACATCTTA 
GCAGTGAGTG ACCAGGAGAC AGGCTCAGAC ACCACCTGCT GGCTGCCTAA TGATGCTCGC 
CGGGAGGTCC ATATAAAAAG GATGGAGGAG AGGAAGGCCT CAAGTACCAC TCCACCTGGT 
GATTCCTTGG CTTCCATCCC ATTTATAGAT GAACCTACCA GCCCTAGCAT CGATCACGAC 
ATTTCTCACA TCCCCGCCTC TGCGGTCATC TCTGCCTCCA CCTCCCAGGT ACCCTCCATA 
GCAACGGTCC CTCCCAGCCT CACAACTTCA GCTCCATTAA TCCGACGTCA GCTCTCCCAT 
GACCAGGAAT CTGTTGGACC TCCCAGCCTG GATGCCCAGC CCAGCTCAAA GACAGAGCGA 
TCCAAATCAT ATGATGAGGG CCTGGATGAT TACAGAGAAG ATGCAAAACT GTCTTTTAAA 
CATGTATCTA GCCTGAAGGG AATCAAGATC TTAGACAGTC AAAAGTCATC AGAAGATTCC 
GGATCCAGGA AAGACTCTTC CTCAGAGGTT TTCAGCGATG CTGCCAAGGA AGGGTGGCTT 
CATTTCAGGC CACTCGTCAC TGATAAGGGC AAGCGCGTTG GTGGAAGTAT TCGGCCATGG 
AAGCAGATGT ATGTTGTACT TCGGGGCCAT TCGCTGTATC TGTACAAAGA TAAACGAGAA 
CAGACGACTC CGTCTGAGGA GGAGCAGCCC ATCAGTGTTA ACGCCTGCTT AATAGACATC 
TCTTACAGCG AGACCAAGAG GAAGAATGTC TTTCGACTCA CCACGTCTGA CTGCGAGTGC 
CTGTTTCAGG CTGAAGACAG GGATGACATG TTAGCCTGGA TCAAAACCAT CCAGGAGAGC 
AGCAATTTAA ACGAGGAGGA CACTGGAGTC ACTAACAGGG ACCTAATTAG TCGAAGAATA 
AAAGAATACA ACAGTCTAAT GAGCAGCAAA GCAGAGCCAT TGCCGAAGAC CCCTCGCCAG 
AGTCTCAGCA TCCGGCAGAC CCTACTTGGT GCTAAAGCAG AGCCTCGGAC TCAGAGTCCA 
CACTCTCCCA AAGAGGAGTC AGAAAGGAAG CTTCTTAGTA AAGATGATAC CAGTCCTCCA 
AAAGACAAAG GCACGTGGAG AAAAGGCATT CCAAGTATTA TGAGAAAGAC ATTTGAGAAA 
AAGCCTACTG CTACGGGAAC ATTCGGGGTC AGACTGGACG ACTGCCCACC AGCTCATACA 
AACCGGTATA TTCCATTAAT AGTTGACATA TGTTGTAAAT TAGTTGAAGA AAGAGGTCTG 
GAATATACAG GTATTTACAG AGTCCCTGGA AACAATGCAG CCATCTCCAG TATGCAGGAG 
GAGCTCAACA AGGGAATGGC TGATATTGAT ATACAAGATG ATAAATGGCG AGATTTGAAT 
GTGATCAGCA GTTTATTAAA ATCCTTCTTC AGAAAACTCC CAGAACCTCT CTTCACGAAT 
GATAAATACG CCGACTTTAT TGAAGCCAAT CGTAAGGAAG ATCCTCTAGA TCGTCTGAAA 
ACATTAAAAA GACTAATTCA TGATTTGCCC GAACATCATT ATGAAACACT CAAGTTTCTT 
TCGGCTCATC TGAAGACAGT AGCAGAAAAT TCAGAAAAAA ATAAGATGGA ACCAAGAAAC 
CTAGCCATAG TGTTTGGTCC AACTCTTGTG AGAACCTCCG AAGATAACAT GACACACATG 
GTCACTCACA TGCCAGATCA GTACAAGATC GTAGAGACAC TCATCCAGCA CCATGACTGG 
TTTTTCACAG AAGAAGGTGC TGAAGAACCT CTTACAACAG TGCAGGAGGA AAACACAGTA 
GACTCCCAGC CAGTGCCAAA CATAGATCAT TTACTCACCA ACATTGGAAG GACAGGCGTC 
TCCCCTGGAG ATGTATCAGA TTCAGCTACT AGTGACTCAA CAAAATCTAA GGGTTCTTGG 
GGATCCGGAA AAGACCAGTA CAGCAAGGAC CTGCTTGTAT CCTCCATCTT CGCGGCTGCC 
AGTCGCAAGA GGAAAAAGCC AAAAGAAAAA GCACAACCCA GCAGCTCGGA AGACGAGCTG 
GACAATGTGT TTTTCAAGAA AGAAAACTCA GAGCAGGGTC ACCACGACAT TAAAGAAGAG 
TCCAAAAAAG AGAGTGAGAC ACCAGGCCGG AAACAAAGGA CCGTTGCTCC CAAAGAAAAC 
AACACGAAGA AAGACAGCAG CGGAGGTGGC AGAGCTGAGC AGAGGGCCCC GCGTGGGGAG 
AGCTTGGAGC CCCCGGCCCC CCAAAGCACC AAGCAGAGCC GGTCACCCAC CCTGAGCTGT 
CGCCTCGCCG TCCTGAGAGA GAGCCCCAGG GCCCTCGCAG CCCAGAAGAC CTCCCACCTG 
GAAGACACGG GGTCCGACTC CAGCACCCCA CTCAGCACTC ACGCCCCGGC TCCCCCGGCA 
AGCTTTCCCA CCAAGAAACC TCCCAGCCCC GAGCCCAAGC ACAGCGAGCT CCTGGTCAGT 
GTCGGCAGCA TCACCTCCGA CCACGCCGGC ACACCGTCTG CTCCCTACCT GGCCGGCCTC 
GACTCCAGCC GGCTGAGCCC CGAGGTGCAG TCCATGGCCG AGAGCCGGGG ATTTGAAGCT 
GATGACGAGA GGAGCGAGCT GGTCAGCGAG GGCCGGCCAG TGGAGACAGA CAGCGAGAGC 
GACTTTCCGG TCTTCCCCGC CGCCCCGGCC TCGGACAGGC TCTTCCGAGG AAAACTCCAA 
GAGGCCGCGA GGACCAGCCG GAGAAACTCA GAAGGCAGTG AGGTCAGCTG CACGGAAGGA 
AGTTTAACAC CAAGTTTAGA AAGCCGGAGA CAGCTCTTCA GCTCCCATAA ACTGATCGAG 
TGTGACACTC TGTCCAGGAA GAAATCCGCC AGGTTCAAGT CAGACAGTGG GAGTCTAGGA 
GATGCCAAGA ATGAGAAAGA AGCCCCTTCC ATCACCAAAG TGTTCGACGT TATGAAAAAA 
GGAAAATCCA CCGGAAATTT ACTGACACCA CCAGCCAGAA GCGAATCGGA CAAACAGGAA 
CCCACTTGGA AAACAAAAAT AGCCGATCGG TTAAAACTGA GGCCCAAAGC CCCGGCCGAT 
GACATGTTTG GAGTAGGAAG TCAAAAAACC ATCGCCGAAC CTGCCAAAAG GAAAAACATC 
AAACGCAGAC ACACGCTGGG TGGGCACAGG GATGCTACTG AAATGAGTGT TCTGAATTTC 
TGGAAAGCCC AGGAGCACAG CGGGGACAGA GAATCCGAAC TTTCAGCTGT GAATCGATTG 
AAGCCCAAAT GCTCCGCCCA GGACCTGTCC ATCTCAGACT GGCTGGCCAG GGAGCGGCTA 
CGCACCAGTA TGACTGACCT GAGCAGAGGG GACACGGGGG ACCCCCGGCC TGAGAGTCTT 
GGCACCTTAG AAATACCAAC CAGGGACCCG CCCCTGTCTT TCCAGTCTGA TGCAGACAGT 
TCTTCCAGCA CCTTGGCTTC AACTAACAGG GCCCCTCTTT CCACACCATC ACAGTCACCT 
GACCAAATAA ATGGAGAAAG CTTCCAGAAC ACGAGCCAAA ACTCCAGTTC TGCAGCCAGG 
GTCCAACCTC ATCAACTGTC TGAAACCCCA GATCACAAAG CACAGTTCCA TCCCTGTCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005194348.1
CDS	121..6003
Translation	MGRAEWRCTDAVIKCSYFLLQKDHMVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_005194291.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005194291.3

ORF Insert Sequence:

ATGGGCCGTG CTGAATGGAG ATGCACAGAT GCTGTTATCA AATGCAGCTA TTTTCTGTTA 
CAGAAAGACC ATATGGTATC AAAAAGTAAA GATGGAAAAG AACAAAGTGA AACTGTATCA 
CCATCCGAAG ATGAAATGTT CTCCTGGCCA GGTCCCAAAA CAGTTATGTT GAAAAGAACA 
TCTCAAGGCT TTGGTTTTAC ATTGAGGCAT TTTATTGTTT ATCCCCCAGA GTCTGCAATT 
CAGTTTTCAT TTAAGGATGA AGAAAATGGC AACAGAGGAG GAAAACAAAG AAACCGTTTG 
GAACCCATGG ACACCATCTT TGTTAAACAG GTGAAAGAGG GCGGACCTGC TTTTGAAGCT 
GGATTATGCA CAGGTGATCG AATTATAAAA GTCAATGGAG AAAGTGTTAT TGGAAAGACC 
TATTCTCAAG TAATTGCTTT AATCCAGAAC AGTGATACAA CATTGGAACT TAGTGTTATG 
CCAAAAGATG AAGACATTCT CCAAGTGCTA CAATTTACAA AGGATGTCAC AGCTCTGGCG 
TATTCTCAAG ATGCCTACCT GAAAGGCAAT GAAGCCTACA GCGGCAATGC CCGCAACATC 
CCTGAGCCCC CACCAGTCTG CTACCCCTGG CCGTCATCCG CCCCTCCCGC CATGGCGCAG 
CCCCCTGAAC CACCTCCTCC AGACTCCACA GCAAGCAAAC CGCAGACTAG CCCCCCAGTA 
TTGACGCAGC CCGGCAGGGC ATACAGGATG GAAATCCAAG TGCCTCCGTC ACCACCAGAG 
GTGGCCAAGT CCAACACAGC AGTGTGTGTC TGCAATGAGA GTGTGAGGAC CGTCATTGTA 
CCTTCTGAGA AGGTTGTAGA TTTATTACCT AACAGAAGCA ACCACACAGT TCCCGCTCAC 
AGAACTGAGG AAGTGAGGTA TGGCCGAAAC GAACAGCCCT CTTTCAAAAC AGCGACGAGA 
ACCACCTCAC CGCCATCCTC AGTTCCCACT GCCCATCTCA TCCACCAGCC CGGCTCCAGG 
TCGTTGGAAC CTTCTGGCAT TTTACTTAAA TCTGGCCACT ACGGCGGACA CTCAGAAGGT 
CTGGTGAGCA GATCTCCAGC TGTGGAGTCT CCTTCCGTAA CTGTCAACCA CTACTCTCCC 
AACTCCCATC AGCACATAGA CTGGAAAAAC TATAAAACTT ACAAAGAGTA TATTGATAAC 
CGGCGCTTGC ACATGGGCTG TCGGACCATT CAAGAAAGGT TAGATAGTTT AAGAGCCGCC 
TCTCAGAGCA CAGCGGATTA TAACCAGGTG GTGCTGAACC GCGCTGCCCT GCAGGTACGG 
CGTCGGAGCA CCTCTCAGGA CCGAGTGCCC CAGTCCATCC AGGTCCGGCA GCGCAGCGTG 
TCCCAGGAGA GGCTGGAGGA CTCTGTGTTG ATGATGTATT GTCCGAGGAG TGCCTCTCAA 
GGTGCGCTGA CATCCCCTTC TATTAGTTTT AGTAACCACA GAACTCGCTC GTGGGATTAC 
ATCGAGGGAC AGGGTGAAAC CTCGGAAAAC GTCCATTCTG AATGTCCACC GCCGGATGCA 
AATGGTGAAC GAAAGCAGAC TTACAAGTGG AGTGGGTTCA CCGAACAGGA CGATCGTCGA 
GGTATTTACG AGAGACCCAG GCAGCAAGAA ATTCACAAAT CTTTCCGAGG CTCCAATTTT 
ACAGTGGCTC CCAGTGTGGT GAATTCTGAC AGCAGGAGAG TGAGCAGCAG AATCGGGGGA 
TCAGTGTCTC AGTTTAAAAA AATTCCAGCA GATCTAAAAC CCCTGCAGCC CAGCAGAAAT 
TTTCAAACTG CTTGTGGAAT ATCCCAGCCT CGAGGGATTT CCCAAGACAG GTCACCTCTT 
GGGAAAGTCC GAAGTAACTC TCTGAAAGGG CCTTCTATGC ATGTCGCGAA ACCGCCCTCC 
AGCCAGAACT CATTTCTTTC TATCAAAGAC CAAAGACCAG TAAATCACTT GCATCAAAAC 
AGTTTATTGA ATCAGCAGCC ATGGTTACGG ACGGAAAGTG CCCCCGATCA CCAAGTGGAC 
ACTGGGAAGC CCCCTTCTCT TTCCGGAGCT TCTGTTAAGC CCCCGGCTCT GGCAAGTGAG 
AATGCTAGCA CTCCAGATTT TGAGTTATCC GTCTCTCAGA GGAATCAAGA TTTAAATGTA 
CAAGAGGTGG AAATTCAGCA ATCGAATGCT GTAGATAATA AAGAAACTGT CATCCTAAGA 
GAAAAACCTC CGTCTGGTCG CCAGACACCG CAGCCTTTAA GGCATCAGTC TTACATCTTA 
GCAGTGAGTG ACCAGGAGAC AGGCTCAGAC ACCACCTGCT GGCTGCCTAA TGATGCTCGC 
CGGGAGGTCC ATATAAAAAG GATGGAGGAG AGGAAGGCCT CAAGTACCAC TCCACCTGGT 
GATTCCTTGG CTTCCATCCC ATTTATAGAT GAACCTACCA GCCCTAGCAT CGATCACGAC 
ATTTCTCACA TCCCCGCCTC TGCGGTCATC TCTGCCTCCA CCTCCCAGGT ACCCTCCATA 
GCAACGGTCC CTCCCAGCCT CACAACTTCA GCTCCATTAA TCCGACGTCA GCTCTCCCAT 
GACCAGGAAT CTGTTGGACC TCCCAGCCTG GATGCCCAGC CCAGCTCAAA GACAGAGCGA 
TCCAAATCAT ATGATGAGGG CCTGGATGAT TACAGAGAAG ATGCAAAACT GTCTTTTAAA 
CATGTATCTA GCCTGAAGGG AATCAAGATC TTAGACAGTC AAAAGTCATC AGAAGATTCC 
GGATCCAGGA AAGACTCTTC CTCAGAGGTT TTCAGCGATG CTGCCAAGGA AGGGTGGCTT 
CATTTCAGGC CACTCGTCAC TGATAAGGGC AAGCGCGTTG GTGGAAGTAT TCGGCCATGG 
AAGCAGATGT ATGTTGTACT TCGGGGCCAT TCGCTGTATC TGTACAAAGA TAAACGAGAA 
CAGACGACTC CGTCTGAGGA GGAGCAGCCC ATCAGTGTTA ACGCCTGCTT AATAGACATC 
TCTTACAGCG AGACCAAGAG GAAGAATGTC TTTCGACTCA CCACGTCTGA CTGCGAGTGC 
CTGTTTCAGG CTGAAGACAG GGATGACATG TTAGCCTGGA TCAAAACCAT CCAGGAGAGC 
AGCAATTTAA ACGAGGAGGA CACTGGAGTC ACTAACAGGG ACCTAATTAG TCGAAGAATA 
AAAGAATACA ACAGTCTAAT GAGCAGCAAA GCAGAGCCAT TGCCGAAGAC CCCTCGCCAG 
AGTCTCAGCA TCCGGCAGAC CCTACTTGGT GCTAAAGCAG AGCCTCGGAC TCAGAGTCCA 
CACTCTCCCA AAGAGGAGTC AGAAAGGAAG CTTCTTAGTA AAGATGATAC CAGTCCTCCA 
AAAGACAAAG GCACGTGGAG AAAAGGCATT CCAAGTATTA TGAGAAAGAC ATTTGAGAAA 
AAGCCTACTG CTACGGGAAC ATTCGGGGTC AGACTGGACG ACTGCCCACC AGCTCATACA 
AACCGGTATA TTCCATTAAT AGTTGACATA TGTTGTAAAT TAGTTGAAGA AAGAGGTCTG 
GAATATACAG GTATTTACAG AGTCCCTGGA AACAATGCAG CCATCTCCAG TATGCAGGAG 
GAGCTCAACA AGGGAATGGC TGATATTGAT ATACAAGATG ATAAATGGCG AGATTTGAAT 
GTGATCAGCA GTTTATTAAA ATCCTTCTTC AGAAAACTCC CAGAACCTCT CTTCACGAAT 
GATAAATACG CCGACTTTAT TGAAGCCAAT CGTAAGGAAG ATCCTCTAGA TCGTCTGAAA 
ACATTAAAAA GACTAATTCA TGATTTGCCC GAACATCATT ATGAAACACT CAAGTTTCTT 
TCGGCTCATC TGAAGACAGT AGCAGAAAAT TCAGAAAAAA ATAAGATGGA ACCAAGAAAC 
CTAGCCATAG TGTTTGGTCC AACTCTTGTG AGAACCTCCG AAGATAACAT GACACACATG 
GTCACTCACA TGCCAGATCA GTACAAGATC GTAGAGACAC TCATCCAGCA CCATGACTGG 
TTTTTCACAG AAGAAGGTGC TGAAGAACCT CTTACAACAG TGCAGGAGGA AAACACAGTA 
GACTCCCAGC CAGTGCCAAA CATAGATCAT TTACTCACCA ACATTGGAAG GACAGGCGTC 
TCCCCTGGAG ATGTATCAGA TTCAGCTACT AGTGACTCAA CAAAATCTAA GGGTTCTTGG 
GGATCCGGAA AAGACCAGTA CAGCAAGGAC CTGCTTGTAT CCTCCATCTT CGCGGCTGCC 
AGTCGCAAGA GGAAAAAGCC AAAAGAAAAA GCACAACCCA GCAGCTCGGA AGACGAGCTG 
GACAATGTGT TTTTCAAGAA AGAAAACTCA GAGCAGGGTC ACCACGACAT TAAAGAAGAG 
TCCAAAAAAG AGAGTGAGAC ACCAGGCCGG AAACAAAGGA CCGTTGCTCC CAAAGAAAAC 
AACACGAAGA AAGACAGCAG CGGAGGTGGC AGAGCTGAGC AGAGGGCCCC GCGTGGGGAG 
AGCTTGGAGC CCCCGGCCCC CCAAAGCACC AAGCAGAGCC GGTCACCCAC CCTGAGCTGT 
CGCCTCGCCG TCCTGAGAGA GAGCCCCAGG GCCCTCGCAG CCCAGAAGAC CTCCCACCTG 
GAAGACACGG GGTCCGACTC CAGCACCCCA CTCAGCACTC ACGCCCCGGC TCCCCCGGCA 
AGCTTTCCCA CCAAGAAACC TCCCAGCCCC GAGCCCAAGC ACAGCGAGCT CCTGGTCAGT 
GTCGGCAGCA TCACCTCCGA CCACGCCGGC ACACCGTCTG CTCCCTACCT GGCCGGCCTC 
GACTCCAGCC GGCTGAGCCC CGAGGTGCAG TCCATGGCCG AGAGCCGGGG ATTTGAAGCT 
GATGACGAGA GGAGCGAGCT GGTCAGCGAG GGCCGGCCAG TGGAGACAGA CAGCGAGAGC 
GACTTTCCGG TCTTCCCCGC CGCCCCGGCC TCGGACAGGC TCTTCCGAGG AAAACTCCAA 
GAGGCCGCGA GGACCAGCCG GAGAAACTCA GAAGGCAGTG AGGTCAGCTG CACGGAAGGA 
AGTTTAACAC CAAGTTTAGA AAGCCGGAGA CAGCTCTTCA GCTCCCATAA ACTGATCGAG 
TGTGACACTC TGTCCAGGAA GAAATCCGCC AGGTTCAAGT CAGACAGTGG GAGTCTAGGA 
GATGCCAAGA ATGAGAAAGA AGCCCCTTCC ATCACCAAAG TGTTCGACGT TATGAAAAAA 
GGAAAATCCA CCGGAAATTT ACTGACACCA CCAGCCAGAA GCGAATCGGA CAAACAGGAA 
CCCACTTGGA AAACAAAAAT AGCCGATCGG TTAAAACTGA GGCCCAAAGC CCCGGCCGAT 
GACATGTTTG GAGTAGGAAG TCAAAAAACC ATCGCCGAAC CTGCCAAAAG GAAAAACATC 
AAACGCAGAC ACACGCTGGG TGGGCACAGG GATGCTACTG AAATGAGTGT TCTGAATTTC 
TGGAAAGCCC AGGAGCACAG CGGGGACAGA GAATCCGAAC TTTCAGCTGT GAATCGATTG 
AAGCCCAAAT GCTCCGCCCA GGACCTGTCC ATCTCAGACT GGCTGGCCAG GGAGCGGCTA 
CGCACCAGTA TGACTGACCT GAGCAGAGGG GACACGGGGG ACCCCCGGCC TGAGAGTCTT 
GGCACCTTAG AAATACCAAC CAGGGACCCG CCCCTGTCTT TCCAGTCTGA TGCAGACAGT 
TCTTCCAGCA CCTTGGCTTC AACTAACAGG GCCCCTCTTT CCACACCATC ACAGTCACCT 
GACCAAATAA ATGGAGAAAG CTTCCAGAAC ACGAGCCAAA ACTCCAGTTC TGCAGCCAGG 
GTCCAACCTC ATCAACTGTC TGAAACCCCA GATCACAAAG CACAGTTCCA TCCCTGTCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_005214302.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214359.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.

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CloneID	OBa07466
Clone ID Related Accession (Same CDS sequence)	XM_025000963.1 , XM_005194291.3 , XM_005214302.3
Accession Version	XM_005214302.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005214302.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGTG CTGAATGGAG ATGCACAGAT GCTGTTATCA AATGCAGCTA TTTTCTGTTA 
CAGAAAGACC ATATGGTATC AAAAAGTAAA GATGGAAAAG AACAAAGTGA AACTGTATCA 
CCATCCGAAG ATGAAATGTT CTCCTGGCCA GGTCCCAAAA CAGTTATGTT GAAAAGAACA 
TCTCAAGGCT TTGGTTTTAC ATTGAGGCAT TTTATTGTTT ATCCCCCAGA GTCTGCAATT 
CAGTTTTCAT TTAAGGATGA AGAAAATGGC AACAGAGGAG GAAAACAAAG AAACCGTTTG 
GAACCCATGG ACACCATCTT TGTTAAACAG GTGAAAGAGG GCGGACCTGC TTTTGAAGCT 
GGATTATGCA CAGGTGATCG AATTATAAAA GTCAATGGAG AAAGTGTTAT TGGAAAGACC 
TATTCTCAAG TAATTGCTTT AATCCAGAAC AGTGATACAA CATTGGAACT TAGTGTTATG 
CCAAAAGATG AAGACATTCT CCAAGTGCTA CAATTTACAA AGGATGTCAC AGCTCTGGCG 
TATTCTCAAG ATGCCTACCT GAAAGGCAAT GAAGCCTACA GCGGCAATGC CCGCAACATC 
CCTGAGCCCC CACCAGTCTG CTACCCCTGG CCGTCATCCG CCCCTCCCGC CATGGCGCAG 
CCCCCTGAAC CACCTCCTCC AGACTCCACA GCAAGCAAAC CGCAGACTAG CCCCCCAGTA 
TTGACGCAGC CCGGCAGGGC ATACAGGATG GAAATCCAAG TGCCTCCGTC ACCACCAGAG 
GTGGCCAAGT CCAACACAGC AGTGTGTGTC TGCAATGAGA GTGTGAGGAC CGTCATTGTA 
CCTTCTGAGA AGGTTGTAGA TTTATTACCT AACAGAAGCA ACCACACAGT TCCCGCTCAC 
AGAACTGAGG AAGTGAGGTA TGGCCGAAAC GAACAGCCCT CTTTCAAAAC AGCGACGAGA 
ACCACCTCAC CGCCATCCTC AGTTCCCACT GCCCATCTCA TCCACCAGCC CGGCTCCAGG 
TCGTTGGAAC CTTCTGGCAT TTTACTTAAA TCTGGCCACT ACGGCGGACA CTCAGAAGGT 
CTGGTGAGCA GATCTCCAGC TGTGGAGTCT CCTTCCGTAA CTGTCAACCA CTACTCTCCC 
AACTCCCATC AGCACATAGA CTGGAAAAAC TATAAAACTT ACAAAGAGTA TATTGATAAC 
CGGCGCTTGC ACATGGGCTG TCGGACCATT CAAGAAAGGT TAGATAGTTT AAGAGCCGCC 
TCTCAGAGCA CAGCGGATTA TAACCAGGTG GTGCTGAACC GCGCTGCCCT GCAGGTACGG 
CGTCGGAGCA CCTCTCAGGA CCGAGTGCCC CAGTCCATCC AGGTCCGGCA GCGCAGCGTG 
TCCCAGGAGA GGCTGGAGGA CTCTGTGTTG ATGATGTATT GTCCGAGGAG TGCCTCTCAA 
GGTGCGCTGA CATCCCCTTC TATTAGTTTT AGTAACCACA GAACTCGCTC GTGGGATTAC 
ATCGAGGGAC AGGGTGAAAC CTCGGAAAAC GTCCATTCTG AATGTCCACC GCCGGATGCA 
AATGGTGAAC GAAAGCAGAC TTACAAGTGG AGTGGGTTCA CCGAACAGGA CGATCGTCGA 
GGTATTTACG AGAGACCCAG GCAGCAAGAA ATTCACAAAT CTTTCCGAGG CTCCAATTTT 
ACAGTGGCTC CCAGTGTGGT GAATTCTGAC AGCAGGAGAG TGAGCAGCAG AATCGGGGGA 
TCAGTGTCTC AGTTTAAAAA AATTCCAGCA GATCTAAAAC CCCTGCAGCC CAGCAGAAAT 
TTTCAAACTG CTTGTGGAAT ATCCCAGCCT CGAGGGATTT CCCAAGACAG GTCACCTCTT 
GGGAAAGTCC GAAGTAACTC TCTGAAAGGG CCTTCTATGC ATGTCGCGAA ACCGCCCTCC 
AGCCAGAACT CATTTCTTTC TATCAAAGAC CAAAGACCAG TAAATCACTT GCATCAAAAC 
AGTTTATTGA ATCAGCAGCC ATGGTTACGG ACGGAAAGTG CCCCCGATCA CCAAGTGGAC 
ACTGGGAAGC CCCCTTCTCT TTCCGGAGCT TCTGTTAAGC CCCCGGCTCT GGCAAGTGAG 
AATGCTAGCA CTCCAGATTT TGAGTTATCC GTCTCTCAGA GGAATCAAGA TTTAAATGTA 
CAAGAGGTGG AAATTCAGCA ATCGAATGCT GTAGATAATA AAGAAACTGT CATCCTAAGA 
GAAAAACCTC CGTCTGGTCG CCAGACACCG CAGCCTTTAA GGCATCAGTC TTACATCTTA 
GCAGTGAGTG ACCAGGAGAC AGGCTCAGAC ACCACCTGCT GGCTGCCTAA TGATGCTCGC 
CGGGAGGTCC ATATAAAAAG GATGGAGGAG AGGAAGGCCT CAAGTACCAC TCCACCTGGT 
GATTCCTTGG CTTCCATCCC ATTTATAGAT GAACCTACCA GCCCTAGCAT CGATCACGAC 
ATTTCTCACA TCCCCGCCTC TGCGGTCATC TCTGCCTCCA CCTCCCAGGT ACCCTCCATA 
GCAACGGTCC CTCCCAGCCT CACAACTTCA GCTCCATTAA TCCGACGTCA GCTCTCCCAT 
GACCAGGAAT CTGTTGGACC TCCCAGCCTG GATGCCCAGC CCAGCTCAAA GACAGAGCGA 
TCCAAATCAT ATGATGAGGG CCTGGATGAT TACAGAGAAG ATGCAAAACT GTCTTTTAAA 
CATGTATCTA GCCTGAAGGG AATCAAGATC TTAGACAGTC AAAAGTCATC AGAAGATTCC 
GGATCCAGGA AAGACTCTTC CTCAGAGGTT TTCAGCGATG CTGCCAAGGA AGGGTGGCTT 
CATTTCAGGC CACTCGTCAC TGATAAGGGC AAGCGCGTTG GTGGAAGTAT TCGGCCATGG 
AAGCAGATGT ATGTTGTACT TCGGGGCCAT TCGCTGTATC TGTACAAAGA TAAACGAGAA 
CAGACGACTC CGTCTGAGGA GGAGCAGCCC ATCAGTGTTA ACGCCTGCTT AATAGACATC 
TCTTACAGCG AGACCAAGAG GAAGAATGTC TTTCGACTCA CCACGTCTGA CTGCGAGTGC 
CTGTTTCAGG CTGAAGACAG GGATGACATG TTAGCCTGGA TCAAAACCAT CCAGGAGAGC 
AGCAATTTAA ACGAGGAGGA CACTGGAGTC ACTAACAGGG ACCTAATTAG TCGAAGAATA 
AAAGAATACA ACAGTCTAAT GAGCAGCAAA GCAGAGCCAT TGCCGAAGAC CCCTCGCCAG 
AGTCTCAGCA TCCGGCAGAC CCTACTTGGT GCTAAAGCAG AGCCTCGGAC TCAGAGTCCA 
CACTCTCCCA AAGAGGAGTC AGAAAGGAAG CTTCTTAGTA AAGATGATAC CAGTCCTCCA 
AAAGACAAAG GCACGTGGAG AAAAGGCATT CCAAGTATTA TGAGAAAGAC ATTTGAGAAA 
AAGCCTACTG CTACGGGAAC ATTCGGGGTC AGACTGGACG ACTGCCCACC AGCTCATACA 
AACCGGTATA TTCCATTAAT AGTTGACATA TGTTGTAAAT TAGTTGAAGA AAGAGGTCTG 
GAATATACAG GTATTTACAG AGTCCCTGGA AACAATGCAG CCATCTCCAG TATGCAGGAG 
GAGCTCAACA AGGGAATGGC TGATATTGAT ATACAAGATG ATAAATGGCG AGATTTGAAT 
GTGATCAGCA GTTTATTAAA ATCCTTCTTC AGAAAACTCC CAGAACCTCT CTTCACGAAT 
GATAAATACG CCGACTTTAT TGAAGCCAAT CGTAAGGAAG ATCCTCTAGA TCGTCTGAAA 
ACATTAAAAA GACTAATTCA TGATTTGCCC GAACATCATT ATGAAACACT CAAGTTTCTT 
TCGGCTCATC TGAAGACAGT AGCAGAAAAT TCAGAAAAAA ATAAGATGGA ACCAAGAAAC 
CTAGCCATAG TGTTTGGTCC AACTCTTGTG AGAACCTCCG AAGATAACAT GACACACATG 
GTCACTCACA TGCCAGATCA GTACAAGATC GTAGAGACAC TCATCCAGCA CCATGACTGG 
TTTTTCACAG AAGAAGGTGC TGAAGAACCT CTTACAACAG TGCAGGAGGA AAACACAGTA 
GACTCCCAGC CAGTGCCAAA CATAGATCAT TTACTCACCA ACATTGGAAG GACAGGCGTC 
TCCCCTGGAG ATGTATCAGA TTCAGCTACT AGTGACTCAA CAAAATCTAA GGGTTCTTGG 
GGATCCGGAA AAGACCAGTA CAGCAAGGAC CTGCTTGTAT CCTCCATCTT CGCGGCTGCC 
AGTCGCAAGA GGAAAAAGCC AAAAGAAAAA GCACAACCCA GCAGCTCGGA AGACGAGCTG 
GACAATGTGT TTTTCAAGAA AGAAAACTCA GAGCAGGGTC ACCACGACAT TAAAGAAGAG 
TCCAAAAAAG AGAGTGAGAC ACCAGGCCGG AAACAAAGGA CCGTTGCTCC CAAAGAAAAC 
AACACGAAGA AAGACAGCAG CGGAGGTGGC AGAGCTGAGC AGAGGGCCCC GCGTGGGGAG 
AGCTTGGAGC CCCCGGCCCC CCAAAGCACC AAGCAGAGCC GGTCACCCAC CCTGAGCTGT 
CGCCTCGCCG TCCTGAGAGA GAGCCCCAGG GCCCTCGCAG CCCAGAAGAC CTCCCACCTG 
GAAGACACGG GGTCCGACTC CAGCACCCCA CTCAGCACTC ACGCCCCGGC TCCCCCGGCA 
AGCTTTCCCA CCAAGAAACC TCCCAGCCCC GAGCCCAAGC ACAGCGAGCT CCTGGTCAGT 
GTCGGCAGCA TCACCTCCGA CCACGCCGGC ACACCGTCTG CTCCCTACCT GGCCGGCCTC 
GACTCCAGCC GGCTGAGCCC CGAGGTGCAG TCCATGGCCG AGAGCCGGGG ATTTGAAGCT 
GATGACGAGA GGAGCGAGCT GGTCAGCGAG GGCCGGCCAG TGGAGACAGA CAGCGAGAGC 
GACTTTCCGG TCTTCCCCGC CGCCCCGGCC TCGGACAGGC TCTTCCGAGG AAAACTCCAA 
GAGGCCGCGA GGACCAGCCG GAGAAACTCA GAAGGCAGTG AGGTCAGCTG CACGGAAGGA 
AGTTTAACAC CAAGTTTAGA AAGCCGGAGA CAGCTCTTCA GCTCCCATAA ACTGATCGAG 
TGTGACACTC TGTCCAGGAA GAAATCCGCC AGGTTCAAGT CAGACAGTGG GAGTCTAGGA 
GATGCCAAGA ATGAGAAAGA AGCCCCTTCC ATCACCAAAG TGTTCGACGT TATGAAAAAA 
GGAAAATCCA CCGGAAATTT ACTGACACCA CCAGCCAGAA GCGAATCGGA CAAACAGGAA 
CCCACTTGGA AAACAAAAAT AGCCGATCGG TTAAAACTGA GGCCCAAAGC CCCGGCCGAT 
GACATGTTTG GAGTAGGAAG TCAAAAAACC ATCGCCGAAC CTGCCAAAAG GAAAAACATC 
AAACGCAGAC ACACGCTGGG TGGGCACAGG GATGCTACTG AAATGAGTGT TCTGAATTTC 
TGGAAAGCCC AGGAGCACAG CGGGGACAGA GAATCCGAAC TTTCAGCTGT GAATCGATTG 
AAGCCCAAAT GCTCCGCCCA GGACCTGTCC ATCTCAGACT GGCTGGCCAG GGAGCGGCTA 
CGCACCAGTA TGACTGACCT GAGCAGAGGG GACACGGGGG ACCCCCGGCC TGAGAGTCTT 
GGCACCTTAG AAATACCAAC CAGGGACCCG CCCCTGTCTT TCCAGTCTGA TGCAGACAGT 
TCTTCCAGCA CCTTGGCTTC AACTAACAGG GCCCCTCTTT CCACACCATC ACAGTCACCT 
GACCAAATAA ATGGAGAAAG CTTCCAGAAC ACGAGCCAAA ACTCCAGTTC TGCAGCCAGG 
GTCCAACCTC ATCAACTGTC TGAAACCCCA GATCACAAAG CACAGTTCCA TCCCTGTCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214359.1
CDS	121..6003
Translation	MGRAEWRCTDAVIKCSYFLLQKDHMVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_005214302.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214302.3

ORF Insert Sequence:

ATGGGCCGTG CTGAATGGAG ATGCACAGAT GCTGTTATCA AATGCAGCTA TTTTCTGTTA 
CAGAAAGACC ATATGGTATC AAAAAGTAAA GATGGAAAAG AACAAAGTGA AACTGTATCA 
CCATCCGAAG ATGAAATGTT CTCCTGGCCA GGTCCCAAAA CAGTTATGTT GAAAAGAACA 
TCTCAAGGCT TTGGTTTTAC ATTGAGGCAT TTTATTGTTT ATCCCCCAGA GTCTGCAATT 
CAGTTTTCAT TTAAGGATGA AGAAAATGGC AACAGAGGAG GAAAACAAAG AAACCGTTTG 
GAACCCATGG ACACCATCTT TGTTAAACAG GTGAAAGAGG GCGGACCTGC TTTTGAAGCT 
GGATTATGCA CAGGTGATCG AATTATAAAA GTCAATGGAG AAAGTGTTAT TGGAAAGACC 
TATTCTCAAG TAATTGCTTT AATCCAGAAC AGTGATACAA CATTGGAACT TAGTGTTATG 
CCAAAAGATG AAGACATTCT CCAAGTGCTA CAATTTACAA AGGATGTCAC AGCTCTGGCG 
TATTCTCAAG ATGCCTACCT GAAAGGCAAT GAAGCCTACA GCGGCAATGC CCGCAACATC 
CCTGAGCCCC CACCAGTCTG CTACCCCTGG CCGTCATCCG CCCCTCCCGC CATGGCGCAG 
CCCCCTGAAC CACCTCCTCC AGACTCCACA GCAAGCAAAC CGCAGACTAG CCCCCCAGTA 
TTGACGCAGC CCGGCAGGGC ATACAGGATG GAAATCCAAG TGCCTCCGTC ACCACCAGAG 
GTGGCCAAGT CCAACACAGC AGTGTGTGTC TGCAATGAGA GTGTGAGGAC CGTCATTGTA 
CCTTCTGAGA AGGTTGTAGA TTTATTACCT AACAGAAGCA ACCACACAGT TCCCGCTCAC 
AGAACTGAGG AAGTGAGGTA TGGCCGAAAC GAACAGCCCT CTTTCAAAAC AGCGACGAGA 
ACCACCTCAC CGCCATCCTC AGTTCCCACT GCCCATCTCA TCCACCAGCC CGGCTCCAGG 
TCGTTGGAAC CTTCTGGCAT TTTACTTAAA TCTGGCCACT ACGGCGGACA CTCAGAAGGT 
CTGGTGAGCA GATCTCCAGC TGTGGAGTCT CCTTCCGTAA CTGTCAACCA CTACTCTCCC 
AACTCCCATC AGCACATAGA CTGGAAAAAC TATAAAACTT ACAAAGAGTA TATTGATAAC 
CGGCGCTTGC ACATGGGCTG TCGGACCATT CAAGAAAGGT TAGATAGTTT AAGAGCCGCC 
TCTCAGAGCA CAGCGGATTA TAACCAGGTG GTGCTGAACC GCGCTGCCCT GCAGGTACGG 
CGTCGGAGCA CCTCTCAGGA CCGAGTGCCC CAGTCCATCC AGGTCCGGCA GCGCAGCGTG 
TCCCAGGAGA GGCTGGAGGA CTCTGTGTTG ATGATGTATT GTCCGAGGAG TGCCTCTCAA 
GGTGCGCTGA CATCCCCTTC TATTAGTTTT AGTAACCACA GAACTCGCTC GTGGGATTAC 
ATCGAGGGAC AGGGTGAAAC CTCGGAAAAC GTCCATTCTG AATGTCCACC GCCGGATGCA 
AATGGTGAAC GAAAGCAGAC TTACAAGTGG AGTGGGTTCA CCGAACAGGA CGATCGTCGA 
GGTATTTACG AGAGACCCAG GCAGCAAGAA ATTCACAAAT CTTTCCGAGG CTCCAATTTT 
ACAGTGGCTC CCAGTGTGGT GAATTCTGAC AGCAGGAGAG TGAGCAGCAG AATCGGGGGA 
TCAGTGTCTC AGTTTAAAAA AATTCCAGCA GATCTAAAAC CCCTGCAGCC CAGCAGAAAT 
TTTCAAACTG CTTGTGGAAT ATCCCAGCCT CGAGGGATTT CCCAAGACAG GTCACCTCTT 
GGGAAAGTCC GAAGTAACTC TCTGAAAGGG CCTTCTATGC ATGTCGCGAA ACCGCCCTCC 
AGCCAGAACT CATTTCTTTC TATCAAAGAC CAAAGACCAG TAAATCACTT GCATCAAAAC 
AGTTTATTGA ATCAGCAGCC ATGGTTACGG ACGGAAAGTG CCCCCGATCA CCAAGTGGAC 
ACTGGGAAGC CCCCTTCTCT TTCCGGAGCT TCTGTTAAGC CCCCGGCTCT GGCAAGTGAG 
AATGCTAGCA CTCCAGATTT TGAGTTATCC GTCTCTCAGA GGAATCAAGA TTTAAATGTA 
CAAGAGGTGG AAATTCAGCA ATCGAATGCT GTAGATAATA AAGAAACTGT CATCCTAAGA 
GAAAAACCTC CGTCTGGTCG CCAGACACCG CAGCCTTTAA GGCATCAGTC TTACATCTTA 
GCAGTGAGTG ACCAGGAGAC AGGCTCAGAC ACCACCTGCT GGCTGCCTAA TGATGCTCGC 
CGGGAGGTCC ATATAAAAAG GATGGAGGAG AGGAAGGCCT CAAGTACCAC TCCACCTGGT 
GATTCCTTGG CTTCCATCCC ATTTATAGAT GAACCTACCA GCCCTAGCAT CGATCACGAC 
ATTTCTCACA TCCCCGCCTC TGCGGTCATC TCTGCCTCCA CCTCCCAGGT ACCCTCCATA 
GCAACGGTCC CTCCCAGCCT CACAACTTCA GCTCCATTAA TCCGACGTCA GCTCTCCCAT 
GACCAGGAAT CTGTTGGACC TCCCAGCCTG GATGCCCAGC CCAGCTCAAA GACAGAGCGA 
TCCAAATCAT ATGATGAGGG CCTGGATGAT TACAGAGAAG ATGCAAAACT GTCTTTTAAA 
CATGTATCTA GCCTGAAGGG AATCAAGATC TTAGACAGTC AAAAGTCATC AGAAGATTCC 
GGATCCAGGA AAGACTCTTC CTCAGAGGTT TTCAGCGATG CTGCCAAGGA AGGGTGGCTT 
CATTTCAGGC CACTCGTCAC TGATAAGGGC AAGCGCGTTG GTGGAAGTAT TCGGCCATGG 
AAGCAGATGT ATGTTGTACT TCGGGGCCAT TCGCTGTATC TGTACAAAGA TAAACGAGAA 
CAGACGACTC CGTCTGAGGA GGAGCAGCCC ATCAGTGTTA ACGCCTGCTT AATAGACATC 
TCTTACAGCG AGACCAAGAG GAAGAATGTC TTTCGACTCA CCACGTCTGA CTGCGAGTGC 
CTGTTTCAGG CTGAAGACAG GGATGACATG TTAGCCTGGA TCAAAACCAT CCAGGAGAGC 
AGCAATTTAA ACGAGGAGGA CACTGGAGTC ACTAACAGGG ACCTAATTAG TCGAAGAATA 
AAAGAATACA ACAGTCTAAT GAGCAGCAAA GCAGAGCCAT TGCCGAAGAC CCCTCGCCAG 
AGTCTCAGCA TCCGGCAGAC CCTACTTGGT GCTAAAGCAG AGCCTCGGAC TCAGAGTCCA 
CACTCTCCCA AAGAGGAGTC AGAAAGGAAG CTTCTTAGTA AAGATGATAC CAGTCCTCCA 
AAAGACAAAG GCACGTGGAG AAAAGGCATT CCAAGTATTA TGAGAAAGAC ATTTGAGAAA 
AAGCCTACTG CTACGGGAAC ATTCGGGGTC AGACTGGACG ACTGCCCACC AGCTCATACA 
AACCGGTATA TTCCATTAAT AGTTGACATA TGTTGTAAAT TAGTTGAAGA AAGAGGTCTG 
GAATATACAG GTATTTACAG AGTCCCTGGA AACAATGCAG CCATCTCCAG TATGCAGGAG 
GAGCTCAACA AGGGAATGGC TGATATTGAT ATACAAGATG ATAAATGGCG AGATTTGAAT 
GTGATCAGCA GTTTATTAAA ATCCTTCTTC AGAAAACTCC CAGAACCTCT CTTCACGAAT 
GATAAATACG CCGACTTTAT TGAAGCCAAT CGTAAGGAAG ATCCTCTAGA TCGTCTGAAA 
ACATTAAAAA GACTAATTCA TGATTTGCCC GAACATCATT ATGAAACACT CAAGTTTCTT 
TCGGCTCATC TGAAGACAGT AGCAGAAAAT TCAGAAAAAA ATAAGATGGA ACCAAGAAAC 
CTAGCCATAG TGTTTGGTCC AACTCTTGTG AGAACCTCCG AAGATAACAT GACACACATG 
GTCACTCACA TGCCAGATCA GTACAAGATC GTAGAGACAC TCATCCAGCA CCATGACTGG 
TTTTTCACAG AAGAAGGTGC TGAAGAACCT CTTACAACAG TGCAGGAGGA AAACACAGTA 
GACTCCCAGC CAGTGCCAAA CATAGATCAT TTACTCACCA ACATTGGAAG GACAGGCGTC 
TCCCCTGGAG ATGTATCAGA TTCAGCTACT AGTGACTCAA CAAAATCTAA GGGTTCTTGG 
GGATCCGGAA AAGACCAGTA CAGCAAGGAC CTGCTTGTAT CCTCCATCTT CGCGGCTGCC 
AGTCGCAAGA GGAAAAAGCC AAAAGAAAAA GCACAACCCA GCAGCTCGGA AGACGAGCTG 
GACAATGTGT TTTTCAAGAA AGAAAACTCA GAGCAGGGTC ACCACGACAT TAAAGAAGAG 
TCCAAAAAAG AGAGTGAGAC ACCAGGCCGG AAACAAAGGA CCGTTGCTCC CAAAGAAAAC 
AACACGAAGA AAGACAGCAG CGGAGGTGGC AGAGCTGAGC AGAGGGCCCC GCGTGGGGAG 
AGCTTGGAGC CCCCGGCCCC CCAAAGCACC AAGCAGAGCC GGTCACCCAC CCTGAGCTGT 
CGCCTCGCCG TCCTGAGAGA GAGCCCCAGG GCCCTCGCAG CCCAGAAGAC CTCCCACCTG 
GAAGACACGG GGTCCGACTC CAGCACCCCA CTCAGCACTC ACGCCCCGGC TCCCCCGGCA 
AGCTTTCCCA CCAAGAAACC TCCCAGCCCC GAGCCCAAGC ACAGCGAGCT CCTGGTCAGT 
GTCGGCAGCA TCACCTCCGA CCACGCCGGC ACACCGTCTG CTCCCTACCT GGCCGGCCTC 
GACTCCAGCC GGCTGAGCCC CGAGGTGCAG TCCATGGCCG AGAGCCGGGG ATTTGAAGCT 
GATGACGAGA GGAGCGAGCT GGTCAGCGAG GGCCGGCCAG TGGAGACAGA CAGCGAGAGC 
GACTTTCCGG TCTTCCCCGC CGCCCCGGCC TCGGACAGGC TCTTCCGAGG AAAACTCCAA 
GAGGCCGCGA GGACCAGCCG GAGAAACTCA GAAGGCAGTG AGGTCAGCTG CACGGAAGGA 
AGTTTAACAC CAAGTTTAGA AAGCCGGAGA CAGCTCTTCA GCTCCCATAA ACTGATCGAG 
TGTGACACTC TGTCCAGGAA GAAATCCGCC AGGTTCAAGT CAGACAGTGG GAGTCTAGGA 
GATGCCAAGA ATGAGAAAGA AGCCCCTTCC ATCACCAAAG TGTTCGACGT TATGAAAAAA 
GGAAAATCCA CCGGAAATTT ACTGACACCA CCAGCCAGAA GCGAATCGGA CAAACAGGAA 
CCCACTTGGA AAACAAAAAT AGCCGATCGG TTAAAACTGA GGCCCAAAGC CCCGGCCGAT 
GACATGTTTG GAGTAGGAAG TCAAAAAACC ATCGCCGAAC CTGCCAAAAG GAAAAACATC 
AAACGCAGAC ACACGCTGGG TGGGCACAGG GATGCTACTG AAATGAGTGT TCTGAATTTC 
TGGAAAGCCC AGGAGCACAG CGGGGACAGA GAATCCGAAC TTTCAGCTGT GAATCGATTG 
AAGCCCAAAT GCTCCGCCCA GGACCTGTCC ATCTCAGACT GGCTGGCCAG GGAGCGGCTA 
CGCACCAGTA TGACTGACCT GAGCAGAGGG GACACGGGGG ACCCCCGGCC TGAGAGTCTT 
GGCACCTTAG AAATACCAAC CAGGGACCCG CCCCTGTCTT TCCAGTCTGA TGCAGACAGT 
TCTTCCAGCA CCTTGGCTTC AACTAACAGG GCCCCTCTTT CCACACCATC ACAGTCACCT 
GACCAAATAA ATGGAGAAAG CTTCCAGAAC ACGAGCCAAA ACTCCAGTTC TGCAGCCAGG 
GTCCAACCTC ATCAACTGTC TGAAACCCCA GATCACAAAG CACAGTTCCA TCCCTGTCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_005194293.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005194350.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X7, mRNA.

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CloneID	OBa07469
Clone ID Related Accession (Same CDS sequence)	XM_005194293.3 , XM_005214304.3
Accession Version	XM_005194293.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646129.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194293.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAAAG ATGAAGACAT TCTCCAAGTG CTACAATTTA CAAAGGATGT CACAGCTCTG 
GCGTATTCTC AAGATGCCTA CCTGAAAGGC AATGAAGCCT ACAGCGGCAA TGCCCGCAAC 
ATCCCTGAGC CCCCACCAGT CTGCTACCCC TGGCCGTCAT CCGCCCCTCC CGCCATGGCG 
CAGCCCCCTG AACCACCTCC TCCAGACTCC ACAGCAAGCA AACCGCAGAC TAGCCCCCCA 
GTATTGACGC AGCCCGGCAG GGCATACAGG ATGGAAATCC AAGTGCCTCC GTCACCACCA 
GAGGTGGCCA AGTCCAACAC AGCAGTGTGT GTCTGCAATG AGAGTGTGAG GACCGTCATT 
GTACCTTCTG AGAAGGTTGT AGATTTATTA CCTAACAGAA GCAACCACAC AGTTCCCGCT 
CACAGAACTG AGGAAGTGAG GTATGGCCGA AACGAACAGC CCTCTTTCAA AACAGCGACG 
AGAACCACCT CACCGCCATC CTCAGTTCCC ACTGCCCATC TCATCCACCA GCCCGGCTCC 
AGGTCGTTGG AACCTTCTGG CATTTTACTT AAATCTGGCC ACTACGGCGG ACACTCAGAA 
GGTCTGGTGA GCAGATCTCC AGCTGTGGAG TCTCCTTCCG TAACTGTCAA CCACTACTCT 
CCCAACTCCC ATCAGCACAT AGACTGGAAA AACTATAAAA CTTACAAAGA GTATATTGAT 
AACCGGCGCT TGCACATGGG CTGTCGGACC ATTCAAGAAA GGTTAGATAG TTTAAGAGCC 
GCCTCTCAGA GCACAGCGGA TTATAACCAG GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA 
CGGCGTCGGA GCACCTCTCA GGACCGAGTG CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC 
GTGTCCCAGG AGAGGCTGGA GGACTCTGTG TTGATGATGT ATTGTCCGAG GAGTGCCTCT 
CAAGGTGCGC TGACATCCCC TTCTATTAGT TTTAGTAACC ACAGAACTCG CTCGTGGGAT 
TACATCGAGG GACAGGGTGA AACCTCGGAA AACGTCCATT CTGAATGTCC ACCGCCGGAT 
GCAAATGGTG AACGAAAGCA GACTTACAAG TGGAGTGGGT TCACCGAACA GGACGATCGT 
CGAGGTATTT ACGAGAGACC CAGGCAGCAA GAAATTCACA AATCTTTCCG AGGCTCCAAT 
TTTACAGTGG CTCCCAGTGT GGTGAATTCT GACAGCAGGA GAGTGAGCAG CAGAATCGGG 
GGATCAGTGT CTCAGTTTAA AAAAATTCCA GCAGATCTAA AACCCCTGCA GCCCAGCAGA 
AATTTTCAAA CTGCTTGTGG AATATCCCAG CCTCGAGGGA TTTCCCAAGA CAGGTCACCT 
CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA GGGCCTTCTA TGCATGTCGC GAAACCGCCC 
TCCAGCCAGA ACTCATTTCT TTCTATCAAA GACCAAAGAC CAGTAAATCA CTTGCATCAA 
AACAGTTTAT TGAATCAGCA GCCATGGTTA CGGACGGAAA GTGCCCCCGA TCACCAAGTG 
GACACTGGGA AGCCCCCTTC TCTTTCCGGA GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT 
GAGAATGCTA GCACTCCAGA TTTTGAGTTA TCCGTCTCTC AGAGGAATCA AGATTTAAAT 
GTACAAGAGG TGGAAATTCA GCAATCGAAT GCTGTAGATA ATAAAGAAAC TGTCATCCTA 
AGAGAAAAAC CTCCGTCTGG TCGCCAGACA CCGCAGCCTT TAAGGCATCA GTCTTACATC 
TTAGCAGTGA GTGACCAGGA GACAGGCTCA GACACCACCT GCTGGCTGCC TAATGATGCT 
CGCCGGGAGG TCCATATAAA AAGGATGGAG GAGAGGAAGG CCTCAAGTAC CACTCCACCT 
GGTGATTCCT TGGCTTCCAT CCCATTTATA GATGAACCTA CCAGCCCTAG CATCGATCAC 
GACATTTCTC ACATCCCCGC CTCTGCGGTC ATCTCTGCCT CCACCTCCCA GGTACCCTCC 
ATAGCAACGG TCCCTCCCAG CCTCACAACT TCAGCTCCAT TAATCCGACG TCAGCTCTCC 
CATGACCAGG AATCTGTTGG ACCTCCCAGC CTGGATGCCC AGCCCAGCTC AAAGACAGAG 
CGATCCAAAT CATATGATGA GGGCCTGGAT GATTACAGAG AAGATGCAAA ACTGTCTTTT 
AAACATGTAT CTAGCCTGAA GGGAATCAAG ATCTTAGACA GTCAAAAGTC ATCAGAAGAT 
TCCGGATCCA GGAAAGACTC TTCCTCAGAG GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG 
CTTCATTTCA GGCCACTCGT CACTGATAAG GGCAAGCGCG TTGGTGGAAG TATTCGGCCA 
TGGAAGCAGA TGTATGTTGT ACTTCGGGGC CATTCGCTGT ATCTGTACAA AGATAAACGA 
GAACAGACGA CTCCGTCTGA GGAGGAGCAG CCCATCAGTG TTAACGCCTG CTTAATAGAC 
ATCTCTTACA GCGAGACCAA GAGGAAGAAT GTCTTTCGAC TCACCACGTC TGACTGCGAG 
TGCCTGTTTC AGGCTGAAGA CAGGGATGAC ATGTTAGCCT GGATCAAAAC CATCCAGGAG 
AGCAGCAATT TAAACGAGGA GGACACTGGA GTCACTAACA GGGACCTAAT TAGTCGAAGA 
ATAAAAGAAT ACAACAGTCT AATGAGCAGC AAAGCAGAGC CATTGCCGAA GACCCCTCGC 
CAGAGTCTCA GCATCCGGCA GACCCTACTT GGTGCTAAAG CAGAGCCTCG GACTCAGAGT 
CCACACTCTC CCAAAGAGGA GTCAGAAAGG AAGCTTCTTA GTAAAGATGA TACCAGTCCT 
CCAAAAGACA AAGGCACGTG GAGAAAAGGC ATTCCAAGTA TTATGAGAAA GACATTTGAG 
AAAAAGCCTA CTGCTACGGG AACATTCGGG GTCAGACTGG ACGACTGCCC ACCAGCTCAT 
ACAAACCGGT ATATTCCATT AATAGTTGAC ATATGTTGTA AATTAGTTGA AGAAAGAGGT 
CTGGAATATA CAGGTATTTA CAGAGTCCCT GGAAACAATG CAGCCATCTC CAGTATGCAG 
GAGGAGCTCA ACAAGGGAAT GGCTGATATT GATATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATCA GCAGTTTATT AAAATCCTTC TTCAGAAAAC TCCCAGAACC TCTCTTCACG 
AATGATAAAT ACGCCGACTT TATTGAAGCC AATCGTAAGG AAGATCCTCT AGATCGTCTG 
AAAACATTAA AAAGACTAAT TCATGATTTG CCCGAACATC ATTATGAAAC ACTCAAGTTT 
CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA AATTCAGAAA AAAATAAGAT GGAACCAAGA 
AACCTAGCCA TAGTGTTTGG TCCAACTCTT GTGAGAACCT CCGAAGATAA CATGACACAC 
ATGGTCACTC ACATGCCAGA TCAGTACAAG ATCGTAGAGA CACTCATCCA GCACCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA CCTCTTACAA CAGTGCAGGA GGAAAACACA 
GTAGACTCCC AGCCAGTGCC AAACATAGAT CATTTACTCA CCAACATTGG AAGGACAGGC 
GTCTCCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CAACAAAATC TAAGGGTTCT 
TGGGGATCCG GAAAAGACCA GTACAGCAAG GACCTGCTTG TATCCTCCAT CTTCGCGGCT 
GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA AAAGCACAAC CCAGCAGCTC GGAAGACGAG 
CTGGACAATG TGTTTTTCAA GAAAGAAAAC TCAGAGCAGG GTCACCACGA CATTAAAGAA 
GAGTCCAAAA AAGAGAGTGA GACACCAGGC CGGAAACAAA GGACCGTTGC TCCCAAAGAA 
AACAACACGA AGAAAGACAG CAGCGGAGGT GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG 
GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC ACCAAGCAGA GCCGGTCACC CACCCTGAGC 
TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC AGGGCCCTCG CAGCCCAGAA GACCTCCCAC 
CTGGAAGACA CGGGGTCCGA CTCCAGCACC CCACTCAGCA CTCACGCCCC GGCTCCCCCG 
GCAAGCTTTC CCACCAAGAA ACCTCCCAGC CCCGAGCCCA AGCACAGCGA GCTCCTGGTC 
AGTGTCGGCA GCATCACCTC CGACCACGCC GGCACACCGT CTGCTCCCTA CCTGGCCGGC 
CTCGACTCCA GCCGGCTGAG CCCCGAGGTG CAGTCCATGG CCGAGAGCCG GGGATTTGAA 
GCTGATGACG AGAGGAGCGA GCTGGTCAGC GAGGGCCGGC CAGTGGAGAC AGACAGCGAG 
AGCGACTTTC CGGTCTTCCC CGCCGCCCCG GCCTCGGACA GGCTCTTCCG AGGAAAACTC 
CAAGAGGCCG CGAGGACCAG CCGGAGAAAC TCAGAAGGCA GTGAGGTCAG CTGCACGGAA 
GGAAGTTTAA CACCAAGTTT AGAAAGCCGG AGACAGCTCT TCAGCTCCCA TAAACTGATC 
GAGTGTGACA CTCTGTCCAG GAAGAAATCC GCCAGGTTCA AGTCAGACAG TGGGAGTCTA 
GGAGATGCCA AGAATGAGAA AGAAGCCCCT TCCATCACCA AAGTGTTCGA CGTTATGAAA 
AAAGGAAAAT CCACCGGAAA TTTACTGACA CCACCAGCCA GAAGCGAATC GGACAAACAG 
GAACCCACTT GGAAAACAAA AATAGCCGAT CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC 
GATGACATGT TTGGAGTAGG AAGTCAAAAA ACCATCGCCG AACCTGCCAA AAGGAAAAAC 
ATCAAACGCA GACACACGCT GGGTGGGCAC AGGGATGCTA CTGAAATGAG TGTTCTGAAT 
TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC AGAGAATCCG AACTTTCAGC TGTGAATCGA 
TTGAAGCCCA AATGCTCCGC CCAGGACCTG TCCATCTCAG ACTGGCTGGC CAGGGAGCGG 
CTACGCACCA GTATGACTGA CCTGAGCAGA GGGGACACGG GGGACCCCCG GCCTGAGAGT 
CTTGGCACCT TAGAAATACC AACCAGGGAC CCGCCCCTGT CTTTCCAGTC TGATGCAGAC 
AGTTCTTCCA GCACCTTGGC TTCAACTAAC AGGGCCCCTC TTTCCACACC ATCACAGTCA 
CCTGACCAAA TAAATGGAGA AAGCTTCCAG AACACGAGCC AAAACTCCAG TTCTGCAGCC 
AGGGTCCAAC CTCATCAACT GTCTGAAACC CCAGATCACA AAGCACAGTT CCATCCCTGT 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005194350.1
CDS	202..5607
Translation	MPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_005194293.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005194293.3

ORF Insert Sequence:

ATGCCAAAAG ATGAAGACAT TCTCCAAGTG CTACAATTTA CAAAGGATGT CACAGCTCTG 
GCGTATTCTC AAGATGCCTA CCTGAAAGGC AATGAAGCCT ACAGCGGCAA TGCCCGCAAC 
ATCCCTGAGC CCCCACCAGT CTGCTACCCC TGGCCGTCAT CCGCCCCTCC CGCCATGGCG 
CAGCCCCCTG AACCACCTCC TCCAGACTCC ACAGCAAGCA AACCGCAGAC TAGCCCCCCA 
GTATTGACGC AGCCCGGCAG GGCATACAGG ATGGAAATCC AAGTGCCTCC GTCACCACCA 
GAGGTGGCCA AGTCCAACAC AGCAGTGTGT GTCTGCAATG AGAGTGTGAG GACCGTCATT 
GTACCTTCTG AGAAGGTTGT AGATTTATTA CCTAACAGAA GCAACCACAC AGTTCCCGCT 
CACAGAACTG AGGAAGTGAG GTATGGCCGA AACGAACAGC CCTCTTTCAA AACAGCGACG 
AGAACCACCT CACCGCCATC CTCAGTTCCC ACTGCCCATC TCATCCACCA GCCCGGCTCC 
AGGTCGTTGG AACCTTCTGG CATTTTACTT AAATCTGGCC ACTACGGCGG ACACTCAGAA 
GGTCTGGTGA GCAGATCTCC AGCTGTGGAG TCTCCTTCCG TAACTGTCAA CCACTACTCT 
CCCAACTCCC ATCAGCACAT AGACTGGAAA AACTATAAAA CTTACAAAGA GTATATTGAT 
AACCGGCGCT TGCACATGGG CTGTCGGACC ATTCAAGAAA GGTTAGATAG TTTAAGAGCC 
GCCTCTCAGA GCACAGCGGA TTATAACCAG GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA 
CGGCGTCGGA GCACCTCTCA GGACCGAGTG CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC 
GTGTCCCAGG AGAGGCTGGA GGACTCTGTG TTGATGATGT ATTGTCCGAG GAGTGCCTCT 
CAAGGTGCGC TGACATCCCC TTCTATTAGT TTTAGTAACC ACAGAACTCG CTCGTGGGAT 
TACATCGAGG GACAGGGTGA AACCTCGGAA AACGTCCATT CTGAATGTCC ACCGCCGGAT 
GCAAATGGTG AACGAAAGCA GACTTACAAG TGGAGTGGGT TCACCGAACA GGACGATCGT 
CGAGGTATTT ACGAGAGACC CAGGCAGCAA GAAATTCACA AATCTTTCCG AGGCTCCAAT 
TTTACAGTGG CTCCCAGTGT GGTGAATTCT GACAGCAGGA GAGTGAGCAG CAGAATCGGG 
GGATCAGTGT CTCAGTTTAA AAAAATTCCA GCAGATCTAA AACCCCTGCA GCCCAGCAGA 
AATTTTCAAA CTGCTTGTGG AATATCCCAG CCTCGAGGGA TTTCCCAAGA CAGGTCACCT 
CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA GGGCCTTCTA TGCATGTCGC GAAACCGCCC 
TCCAGCCAGA ACTCATTTCT TTCTATCAAA GACCAAAGAC CAGTAAATCA CTTGCATCAA 
AACAGTTTAT TGAATCAGCA GCCATGGTTA CGGACGGAAA GTGCCCCCGA TCACCAAGTG 
GACACTGGGA AGCCCCCTTC TCTTTCCGGA GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT 
GAGAATGCTA GCACTCCAGA TTTTGAGTTA TCCGTCTCTC AGAGGAATCA AGATTTAAAT 
GTACAAGAGG TGGAAATTCA GCAATCGAAT GCTGTAGATA ATAAAGAAAC TGTCATCCTA 
AGAGAAAAAC CTCCGTCTGG TCGCCAGACA CCGCAGCCTT TAAGGCATCA GTCTTACATC 
TTAGCAGTGA GTGACCAGGA GACAGGCTCA GACACCACCT GCTGGCTGCC TAATGATGCT 
CGCCGGGAGG TCCATATAAA AAGGATGGAG GAGAGGAAGG CCTCAAGTAC CACTCCACCT 
GGTGATTCCT TGGCTTCCAT CCCATTTATA GATGAACCTA CCAGCCCTAG CATCGATCAC 
GACATTTCTC ACATCCCCGC CTCTGCGGTC ATCTCTGCCT CCACCTCCCA GGTACCCTCC 
ATAGCAACGG TCCCTCCCAG CCTCACAACT TCAGCTCCAT TAATCCGACG TCAGCTCTCC 
CATGACCAGG AATCTGTTGG ACCTCCCAGC CTGGATGCCC AGCCCAGCTC AAAGACAGAG 
CGATCCAAAT CATATGATGA GGGCCTGGAT GATTACAGAG AAGATGCAAA ACTGTCTTTT 
AAACATGTAT CTAGCCTGAA GGGAATCAAG ATCTTAGACA GTCAAAAGTC ATCAGAAGAT 
TCCGGATCCA GGAAAGACTC TTCCTCAGAG GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG 
CTTCATTTCA GGCCACTCGT CACTGATAAG GGCAAGCGCG TTGGTGGAAG TATTCGGCCA 
TGGAAGCAGA TGTATGTTGT ACTTCGGGGC CATTCGCTGT ATCTGTACAA AGATAAACGA 
GAACAGACGA CTCCGTCTGA GGAGGAGCAG CCCATCAGTG TTAACGCCTG CTTAATAGAC 
ATCTCTTACA GCGAGACCAA GAGGAAGAAT GTCTTTCGAC TCACCACGTC TGACTGCGAG 
TGCCTGTTTC AGGCTGAAGA CAGGGATGAC ATGTTAGCCT GGATCAAAAC CATCCAGGAG 
AGCAGCAATT TAAACGAGGA GGACACTGGA GTCACTAACA GGGACCTAAT TAGTCGAAGA 
ATAAAAGAAT ACAACAGTCT AATGAGCAGC AAAGCAGAGC CATTGCCGAA GACCCCTCGC 
CAGAGTCTCA GCATCCGGCA GACCCTACTT GGTGCTAAAG CAGAGCCTCG GACTCAGAGT 
CCACACTCTC CCAAAGAGGA GTCAGAAAGG AAGCTTCTTA GTAAAGATGA TACCAGTCCT 
CCAAAAGACA AAGGCACGTG GAGAAAAGGC ATTCCAAGTA TTATGAGAAA GACATTTGAG 
AAAAAGCCTA CTGCTACGGG AACATTCGGG GTCAGACTGG ACGACTGCCC ACCAGCTCAT 
ACAAACCGGT ATATTCCATT AATAGTTGAC ATATGTTGTA AATTAGTTGA AGAAAGAGGT 
CTGGAATATA CAGGTATTTA CAGAGTCCCT GGAAACAATG CAGCCATCTC CAGTATGCAG 
GAGGAGCTCA ACAAGGGAAT GGCTGATATT GATATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATCA GCAGTTTATT AAAATCCTTC TTCAGAAAAC TCCCAGAACC TCTCTTCACG 
AATGATAAAT ACGCCGACTT TATTGAAGCC AATCGTAAGG AAGATCCTCT AGATCGTCTG 
AAAACATTAA AAAGACTAAT TCATGATTTG CCCGAACATC ATTATGAAAC ACTCAAGTTT 
CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA AATTCAGAAA AAAATAAGAT GGAACCAAGA 
AACCTAGCCA TAGTGTTTGG TCCAACTCTT GTGAGAACCT CCGAAGATAA CATGACACAC 
ATGGTCACTC ACATGCCAGA TCAGTACAAG ATCGTAGAGA CACTCATCCA GCACCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA CCTCTTACAA CAGTGCAGGA GGAAAACACA 
GTAGACTCCC AGCCAGTGCC AAACATAGAT CATTTACTCA CCAACATTGG AAGGACAGGC 
GTCTCCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CAACAAAATC TAAGGGTTCT 
TGGGGATCCG GAAAAGACCA GTACAGCAAG GACCTGCTTG TATCCTCCAT CTTCGCGGCT 
GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA AAAGCACAAC CCAGCAGCTC GGAAGACGAG 
CTGGACAATG TGTTTTTCAA GAAAGAAAAC TCAGAGCAGG GTCACCACGA CATTAAAGAA 
GAGTCCAAAA AAGAGAGTGA GACACCAGGC CGGAAACAAA GGACCGTTGC TCCCAAAGAA 
AACAACACGA AGAAAGACAG CAGCGGAGGT GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG 
GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC ACCAAGCAGA GCCGGTCACC CACCCTGAGC 
TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC AGGGCCCTCG CAGCCCAGAA GACCTCCCAC 
CTGGAAGACA CGGGGTCCGA CTCCAGCACC CCACTCAGCA CTCACGCCCC GGCTCCCCCG 
GCAAGCTTTC CCACCAAGAA ACCTCCCAGC CCCGAGCCCA AGCACAGCGA GCTCCTGGTC 
AGTGTCGGCA GCATCACCTC CGACCACGCC GGCACACCGT CTGCTCCCTA CCTGGCCGGC 
CTCGACTCCA GCCGGCTGAG CCCCGAGGTG CAGTCCATGG CCGAGAGCCG GGGATTTGAA 
GCTGATGACG AGAGGAGCGA GCTGGTCAGC GAGGGCCGGC CAGTGGAGAC AGACAGCGAG 
AGCGACTTTC CGGTCTTCCC CGCCGCCCCG GCCTCGGACA GGCTCTTCCG AGGAAAACTC 
CAAGAGGCCG CGAGGACCAG CCGGAGAAAC TCAGAAGGCA GTGAGGTCAG CTGCACGGAA 
GGAAGTTTAA CACCAAGTTT AGAAAGCCGG AGACAGCTCT TCAGCTCCCA TAAACTGATC 
GAGTGTGACA CTCTGTCCAG GAAGAAATCC GCCAGGTTCA AGTCAGACAG TGGGAGTCTA 
GGAGATGCCA AGAATGAGAA AGAAGCCCCT TCCATCACCA AAGTGTTCGA CGTTATGAAA 
AAAGGAAAAT CCACCGGAAA TTTACTGACA CCACCAGCCA GAAGCGAATC GGACAAACAG 
GAACCCACTT GGAAAACAAA AATAGCCGAT CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC 
GATGACATGT TTGGAGTAGG AAGTCAAAAA ACCATCGCCG AACCTGCCAA AAGGAAAAAC 
ATCAAACGCA GACACACGCT GGGTGGGCAC AGGGATGCTA CTGAAATGAG TGTTCTGAAT 
TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC AGAGAATCCG AACTTTCAGC TGTGAATCGA 
TTGAAGCCCA AATGCTCCGC CCAGGACCTG TCCATCTCAG ACTGGCTGGC CAGGGAGCGG 
CTACGCACCA GTATGACTGA CCTGAGCAGA GGGGACACGG GGGACCCCCG GCCTGAGAGT 
CTTGGCACCT TAGAAATACC AACCAGGGAC CCGCCCCTGT CTTTCCAGTC TGATGCAGAC 
AGTTCTTCCA GCACCTTGGC TTCAACTAAC AGGGCCCCTC TTTCCACACC ATCACAGTCA 
CCTGACCAAA TAAATGGAGA AAGCTTCCAG AACACGAGCC AAAACTCCAG TTCTGCAGCC 
AGGGTCCAAC CTCATCAACT GTCTGAAACC CCAGATCACA AAGCACAGTT CCATCCCTGT 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_005214304.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214361.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X10, mRNA.

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CloneID	OBa07469
Clone ID Related Accession (Same CDS sequence)	XM_005194293.3 , XM_005214304.3
Accession Version	XM_005214304.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005214304.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAAAG ATGAAGACAT TCTCCAAGTG CTACAATTTA CAAAGGATGT CACAGCTCTG 
GCGTATTCTC AAGATGCCTA CCTGAAAGGC AATGAAGCCT ACAGCGGCAA TGCCCGCAAC 
ATCCCTGAGC CCCCACCAGT CTGCTACCCC TGGCCGTCAT CCGCCCCTCC CGCCATGGCG 
CAGCCCCCTG AACCACCTCC TCCAGACTCC ACAGCAAGCA AACCGCAGAC TAGCCCCCCA 
GTATTGACGC AGCCCGGCAG GGCATACAGG ATGGAAATCC AAGTGCCTCC GTCACCACCA 
GAGGTGGCCA AGTCCAACAC AGCAGTGTGT GTCTGCAATG AGAGTGTGAG GACCGTCATT 
GTACCTTCTG AGAAGGTTGT AGATTTATTA CCTAACAGAA GCAACCACAC AGTTCCCGCT 
CACAGAACTG AGGAAGTGAG GTATGGCCGA AACGAACAGC CCTCTTTCAA AACAGCGACG 
AGAACCACCT CACCGCCATC CTCAGTTCCC ACTGCCCATC TCATCCACCA GCCCGGCTCC 
AGGTCGTTGG AACCTTCTGG CATTTTACTT AAATCTGGCC ACTACGGCGG ACACTCAGAA 
GGTCTGGTGA GCAGATCTCC AGCTGTGGAG TCTCCTTCCG TAACTGTCAA CCACTACTCT 
CCCAACTCCC ATCAGCACAT AGACTGGAAA AACTATAAAA CTTACAAAGA GTATATTGAT 
AACCGGCGCT TGCACATGGG CTGTCGGACC ATTCAAGAAA GGTTAGATAG TTTAAGAGCC 
GCCTCTCAGA GCACAGCGGA TTATAACCAG GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA 
CGGCGTCGGA GCACCTCTCA GGACCGAGTG CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC 
GTGTCCCAGG AGAGGCTGGA GGACTCTGTG TTGATGATGT ATTGTCCGAG GAGTGCCTCT 
CAAGGTGCGC TGACATCCCC TTCTATTAGT TTTAGTAACC ACAGAACTCG CTCGTGGGAT 
TACATCGAGG GACAGGGTGA AACCTCGGAA AACGTCCATT CTGAATGTCC ACCGCCGGAT 
GCAAATGGTG AACGAAAGCA GACTTACAAG TGGAGTGGGT TCACCGAACA GGACGATCGT 
CGAGGTATTT ACGAGAGACC CAGGCAGCAA GAAATTCACA AATCTTTCCG AGGCTCCAAT 
TTTACAGTGG CTCCCAGTGT GGTGAATTCT GACAGCAGGA GAGTGAGCAG CAGAATCGGG 
GGATCAGTGT CTCAGTTTAA AAAAATTCCA GCAGATCTAA AACCCCTGCA GCCCAGCAGA 
AATTTTCAAA CTGCTTGTGG AATATCCCAG CCTCGAGGGA TTTCCCAAGA CAGGTCACCT 
CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA GGGCCTTCTA TGCATGTCGC GAAACCGCCC 
TCCAGCCAGA ACTCATTTCT TTCTATCAAA GACCAAAGAC CAGTAAATCA CTTGCATCAA 
AACAGTTTAT TGAATCAGCA GCCATGGTTA CGGACGGAAA GTGCCCCCGA TCACCAAGTG 
GACACTGGGA AGCCCCCTTC TCTTTCCGGA GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT 
GAGAATGCTA GCACTCCAGA TTTTGAGTTA TCCGTCTCTC AGAGGAATCA AGATTTAAAT 
GTACAAGAGG TGGAAATTCA GCAATCGAAT GCTGTAGATA ATAAAGAAAC TGTCATCCTA 
AGAGAAAAAC CTCCGTCTGG TCGCCAGACA CCGCAGCCTT TAAGGCATCA GTCTTACATC 
TTAGCAGTGA GTGACCAGGA GACAGGCTCA GACACCACCT GCTGGCTGCC TAATGATGCT 
CGCCGGGAGG TCCATATAAA AAGGATGGAG GAGAGGAAGG CCTCAAGTAC CACTCCACCT 
GGTGATTCCT TGGCTTCCAT CCCATTTATA GATGAACCTA CCAGCCCTAG CATCGATCAC 
GACATTTCTC ACATCCCCGC CTCTGCGGTC ATCTCTGCCT CCACCTCCCA GGTACCCTCC 
ATAGCAACGG TCCCTCCCAG CCTCACAACT TCAGCTCCAT TAATCCGACG TCAGCTCTCC 
CATGACCAGG AATCTGTTGG ACCTCCCAGC CTGGATGCCC AGCCCAGCTC AAAGACAGAG 
CGATCCAAAT CATATGATGA GGGCCTGGAT GATTACAGAG AAGATGCAAA ACTGTCTTTT 
AAACATGTAT CTAGCCTGAA GGGAATCAAG ATCTTAGACA GTCAAAAGTC ATCAGAAGAT 
TCCGGATCCA GGAAAGACTC TTCCTCAGAG GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG 
CTTCATTTCA GGCCACTCGT CACTGATAAG GGCAAGCGCG TTGGTGGAAG TATTCGGCCA 
TGGAAGCAGA TGTATGTTGT ACTTCGGGGC CATTCGCTGT ATCTGTACAA AGATAAACGA 
GAACAGACGA CTCCGTCTGA GGAGGAGCAG CCCATCAGTG TTAACGCCTG CTTAATAGAC 
ATCTCTTACA GCGAGACCAA GAGGAAGAAT GTCTTTCGAC TCACCACGTC TGACTGCGAG 
TGCCTGTTTC AGGCTGAAGA CAGGGATGAC ATGTTAGCCT GGATCAAAAC CATCCAGGAG 
AGCAGCAATT TAAACGAGGA GGACACTGGA GTCACTAACA GGGACCTAAT TAGTCGAAGA 
ATAAAAGAAT ACAACAGTCT AATGAGCAGC AAAGCAGAGC CATTGCCGAA GACCCCTCGC 
CAGAGTCTCA GCATCCGGCA GACCCTACTT GGTGCTAAAG CAGAGCCTCG GACTCAGAGT 
CCACACTCTC CCAAAGAGGA GTCAGAAAGG AAGCTTCTTA GTAAAGATGA TACCAGTCCT 
CCAAAAGACA AAGGCACGTG GAGAAAAGGC ATTCCAAGTA TTATGAGAAA GACATTTGAG 
AAAAAGCCTA CTGCTACGGG AACATTCGGG GTCAGACTGG ACGACTGCCC ACCAGCTCAT 
ACAAACCGGT ATATTCCATT AATAGTTGAC ATATGTTGTA AATTAGTTGA AGAAAGAGGT 
CTGGAATATA CAGGTATTTA CAGAGTCCCT GGAAACAATG CAGCCATCTC CAGTATGCAG 
GAGGAGCTCA ACAAGGGAAT GGCTGATATT GATATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATCA GCAGTTTATT AAAATCCTTC TTCAGAAAAC TCCCAGAACC TCTCTTCACG 
AATGATAAAT ACGCCGACTT TATTGAAGCC AATCGTAAGG AAGATCCTCT AGATCGTCTG 
AAAACATTAA AAAGACTAAT TCATGATTTG CCCGAACATC ATTATGAAAC ACTCAAGTTT 
CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA AATTCAGAAA AAAATAAGAT GGAACCAAGA 
AACCTAGCCA TAGTGTTTGG TCCAACTCTT GTGAGAACCT CCGAAGATAA CATGACACAC 
ATGGTCACTC ACATGCCAGA TCAGTACAAG ATCGTAGAGA CACTCATCCA GCACCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA CCTCTTACAA CAGTGCAGGA GGAAAACACA 
GTAGACTCCC AGCCAGTGCC AAACATAGAT CATTTACTCA CCAACATTGG AAGGACAGGC 
GTCTCCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CAACAAAATC TAAGGGTTCT 
TGGGGATCCG GAAAAGACCA GTACAGCAAG GACCTGCTTG TATCCTCCAT CTTCGCGGCT 
GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA AAAGCACAAC CCAGCAGCTC GGAAGACGAG 
CTGGACAATG TGTTTTTCAA GAAAGAAAAC TCAGAGCAGG GTCACCACGA CATTAAAGAA 
GAGTCCAAAA AAGAGAGTGA GACACCAGGC CGGAAACAAA GGACCGTTGC TCCCAAAGAA 
AACAACACGA AGAAAGACAG CAGCGGAGGT GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG 
GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC ACCAAGCAGA GCCGGTCACC CACCCTGAGC 
TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC AGGGCCCTCG CAGCCCAGAA GACCTCCCAC 
CTGGAAGACA CGGGGTCCGA CTCCAGCACC CCACTCAGCA CTCACGCCCC GGCTCCCCCG 
GCAAGCTTTC CCACCAAGAA ACCTCCCAGC CCCGAGCCCA AGCACAGCGA GCTCCTGGTC 
AGTGTCGGCA GCATCACCTC CGACCACGCC GGCACACCGT CTGCTCCCTA CCTGGCCGGC 
CTCGACTCCA GCCGGCTGAG CCCCGAGGTG CAGTCCATGG CCGAGAGCCG GGGATTTGAA 
GCTGATGACG AGAGGAGCGA GCTGGTCAGC GAGGGCCGGC CAGTGGAGAC AGACAGCGAG 
AGCGACTTTC CGGTCTTCCC CGCCGCCCCG GCCTCGGACA GGCTCTTCCG AGGAAAACTC 
CAAGAGGCCG CGAGGACCAG CCGGAGAAAC TCAGAAGGCA GTGAGGTCAG CTGCACGGAA 
GGAAGTTTAA CACCAAGTTT AGAAAGCCGG AGACAGCTCT TCAGCTCCCA TAAACTGATC 
GAGTGTGACA CTCTGTCCAG GAAGAAATCC GCCAGGTTCA AGTCAGACAG TGGGAGTCTA 
GGAGATGCCA AGAATGAGAA AGAAGCCCCT TCCATCACCA AAGTGTTCGA CGTTATGAAA 
AAAGGAAAAT CCACCGGAAA TTTACTGACA CCACCAGCCA GAAGCGAATC GGACAAACAG 
GAACCCACTT GGAAAACAAA AATAGCCGAT CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC 
GATGACATGT TTGGAGTAGG AAGTCAAAAA ACCATCGCCG AACCTGCCAA AAGGAAAAAC 
ATCAAACGCA GACACACGCT GGGTGGGCAC AGGGATGCTA CTGAAATGAG TGTTCTGAAT 
TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC AGAGAATCCG AACTTTCAGC TGTGAATCGA 
TTGAAGCCCA AATGCTCCGC CCAGGACCTG TCCATCTCAG ACTGGCTGGC CAGGGAGCGG 
CTACGCACCA GTATGACTGA CCTGAGCAGA GGGGACACGG GGGACCCCCG GCCTGAGAGT 
CTTGGCACCT TAGAAATACC AACCAGGGAC CCGCCCCTGT CTTTCCAGTC TGATGCAGAC 
AGTTCTTCCA GCACCTTGGC TTCAACTAAC AGGGCCCCTC TTTCCACACC ATCACAGTCA 
CCTGACCAAA TAAATGGAGA AAGCTTCCAG AACACGAGCC AAAACTCCAG TTCTGCAGCC 
AGGGTCCAAC CTCATCAACT GTCTGAAACC CCAGATCACA AAGCACAGTT CCATCCCTGT 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214361.1
CDS	202..5607
Translation	MPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_005214304.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214304.3

ORF Insert Sequence:

ATGCCAAAAG ATGAAGACAT TCTCCAAGTG CTACAATTTA CAAAGGATGT CACAGCTCTG 
GCGTATTCTC AAGATGCCTA CCTGAAAGGC AATGAAGCCT ACAGCGGCAA TGCCCGCAAC 
ATCCCTGAGC CCCCACCAGT CTGCTACCCC TGGCCGTCAT CCGCCCCTCC CGCCATGGCG 
CAGCCCCCTG AACCACCTCC TCCAGACTCC ACAGCAAGCA AACCGCAGAC TAGCCCCCCA 
GTATTGACGC AGCCCGGCAG GGCATACAGG ATGGAAATCC AAGTGCCTCC GTCACCACCA 
GAGGTGGCCA AGTCCAACAC AGCAGTGTGT GTCTGCAATG AGAGTGTGAG GACCGTCATT 
GTACCTTCTG AGAAGGTTGT AGATTTATTA CCTAACAGAA GCAACCACAC AGTTCCCGCT 
CACAGAACTG AGGAAGTGAG GTATGGCCGA AACGAACAGC CCTCTTTCAA AACAGCGACG 
AGAACCACCT CACCGCCATC CTCAGTTCCC ACTGCCCATC TCATCCACCA GCCCGGCTCC 
AGGTCGTTGG AACCTTCTGG CATTTTACTT AAATCTGGCC ACTACGGCGG ACACTCAGAA 
GGTCTGGTGA GCAGATCTCC AGCTGTGGAG TCTCCTTCCG TAACTGTCAA CCACTACTCT 
CCCAACTCCC ATCAGCACAT AGACTGGAAA AACTATAAAA CTTACAAAGA GTATATTGAT 
AACCGGCGCT TGCACATGGG CTGTCGGACC ATTCAAGAAA GGTTAGATAG TTTAAGAGCC 
GCCTCTCAGA GCACAGCGGA TTATAACCAG GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA 
CGGCGTCGGA GCACCTCTCA GGACCGAGTG CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC 
GTGTCCCAGG AGAGGCTGGA GGACTCTGTG TTGATGATGT ATTGTCCGAG GAGTGCCTCT 
CAAGGTGCGC TGACATCCCC TTCTATTAGT TTTAGTAACC ACAGAACTCG CTCGTGGGAT 
TACATCGAGG GACAGGGTGA AACCTCGGAA AACGTCCATT CTGAATGTCC ACCGCCGGAT 
GCAAATGGTG AACGAAAGCA GACTTACAAG TGGAGTGGGT TCACCGAACA GGACGATCGT 
CGAGGTATTT ACGAGAGACC CAGGCAGCAA GAAATTCACA AATCTTTCCG AGGCTCCAAT 
TTTACAGTGG CTCCCAGTGT GGTGAATTCT GACAGCAGGA GAGTGAGCAG CAGAATCGGG 
GGATCAGTGT CTCAGTTTAA AAAAATTCCA GCAGATCTAA AACCCCTGCA GCCCAGCAGA 
AATTTTCAAA CTGCTTGTGG AATATCCCAG CCTCGAGGGA TTTCCCAAGA CAGGTCACCT 
CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA GGGCCTTCTA TGCATGTCGC GAAACCGCCC 
TCCAGCCAGA ACTCATTTCT TTCTATCAAA GACCAAAGAC CAGTAAATCA CTTGCATCAA 
AACAGTTTAT TGAATCAGCA GCCATGGTTA CGGACGGAAA GTGCCCCCGA TCACCAAGTG 
GACACTGGGA AGCCCCCTTC TCTTTCCGGA GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT 
GAGAATGCTA GCACTCCAGA TTTTGAGTTA TCCGTCTCTC AGAGGAATCA AGATTTAAAT 
GTACAAGAGG TGGAAATTCA GCAATCGAAT GCTGTAGATA ATAAAGAAAC TGTCATCCTA 
AGAGAAAAAC CTCCGTCTGG TCGCCAGACA CCGCAGCCTT TAAGGCATCA GTCTTACATC 
TTAGCAGTGA GTGACCAGGA GACAGGCTCA GACACCACCT GCTGGCTGCC TAATGATGCT 
CGCCGGGAGG TCCATATAAA AAGGATGGAG GAGAGGAAGG CCTCAAGTAC CACTCCACCT 
GGTGATTCCT TGGCTTCCAT CCCATTTATA GATGAACCTA CCAGCCCTAG CATCGATCAC 
GACATTTCTC ACATCCCCGC CTCTGCGGTC ATCTCTGCCT CCACCTCCCA GGTACCCTCC 
ATAGCAACGG TCCCTCCCAG CCTCACAACT TCAGCTCCAT TAATCCGACG TCAGCTCTCC 
CATGACCAGG AATCTGTTGG ACCTCCCAGC CTGGATGCCC AGCCCAGCTC AAAGACAGAG 
CGATCCAAAT CATATGATGA GGGCCTGGAT GATTACAGAG AAGATGCAAA ACTGTCTTTT 
AAACATGTAT CTAGCCTGAA GGGAATCAAG ATCTTAGACA GTCAAAAGTC ATCAGAAGAT 
TCCGGATCCA GGAAAGACTC TTCCTCAGAG GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG 
CTTCATTTCA GGCCACTCGT CACTGATAAG GGCAAGCGCG TTGGTGGAAG TATTCGGCCA 
TGGAAGCAGA TGTATGTTGT ACTTCGGGGC CATTCGCTGT ATCTGTACAA AGATAAACGA 
GAACAGACGA CTCCGTCTGA GGAGGAGCAG CCCATCAGTG TTAACGCCTG CTTAATAGAC 
ATCTCTTACA GCGAGACCAA GAGGAAGAAT GTCTTTCGAC TCACCACGTC TGACTGCGAG 
TGCCTGTTTC AGGCTGAAGA CAGGGATGAC ATGTTAGCCT GGATCAAAAC CATCCAGGAG 
AGCAGCAATT TAAACGAGGA GGACACTGGA GTCACTAACA GGGACCTAAT TAGTCGAAGA 
ATAAAAGAAT ACAACAGTCT AATGAGCAGC AAAGCAGAGC CATTGCCGAA GACCCCTCGC 
CAGAGTCTCA GCATCCGGCA GACCCTACTT GGTGCTAAAG CAGAGCCTCG GACTCAGAGT 
CCACACTCTC CCAAAGAGGA GTCAGAAAGG AAGCTTCTTA GTAAAGATGA TACCAGTCCT 
CCAAAAGACA AAGGCACGTG GAGAAAAGGC ATTCCAAGTA TTATGAGAAA GACATTTGAG 
AAAAAGCCTA CTGCTACGGG AACATTCGGG GTCAGACTGG ACGACTGCCC ACCAGCTCAT 
ACAAACCGGT ATATTCCATT AATAGTTGAC ATATGTTGTA AATTAGTTGA AGAAAGAGGT 
CTGGAATATA CAGGTATTTA CAGAGTCCCT GGAAACAATG CAGCCATCTC CAGTATGCAG 
GAGGAGCTCA ACAAGGGAAT GGCTGATATT GATATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATCA GCAGTTTATT AAAATCCTTC TTCAGAAAAC TCCCAGAACC TCTCTTCACG 
AATGATAAAT ACGCCGACTT TATTGAAGCC AATCGTAAGG AAGATCCTCT AGATCGTCTG 
AAAACATTAA AAAGACTAAT TCATGATTTG CCCGAACATC ATTATGAAAC ACTCAAGTTT 
CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA AATTCAGAAA AAAATAAGAT GGAACCAAGA 
AACCTAGCCA TAGTGTTTGG TCCAACTCTT GTGAGAACCT CCGAAGATAA CATGACACAC 
ATGGTCACTC ACATGCCAGA TCAGTACAAG ATCGTAGAGA CACTCATCCA GCACCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA CCTCTTACAA CAGTGCAGGA GGAAAACACA 
GTAGACTCCC AGCCAGTGCC AAACATAGAT CATTTACTCA CCAACATTGG AAGGACAGGC 
GTCTCCCCTG GAGATGTATC AGATTCAGCT ACTAGTGACT CAACAAAATC TAAGGGTTCT 
TGGGGATCCG GAAAAGACCA GTACAGCAAG GACCTGCTTG TATCCTCCAT CTTCGCGGCT 
GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA AAAGCACAAC CCAGCAGCTC GGAAGACGAG 
CTGGACAATG TGTTTTTCAA GAAAGAAAAC TCAGAGCAGG GTCACCACGA CATTAAAGAA 
GAGTCCAAAA AAGAGAGTGA GACACCAGGC CGGAAACAAA GGACCGTTGC TCCCAAAGAA 
AACAACACGA AGAAAGACAG CAGCGGAGGT GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG 
GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC ACCAAGCAGA GCCGGTCACC CACCCTGAGC 
TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC AGGGCCCTCG CAGCCCAGAA GACCTCCCAC 
CTGGAAGACA CGGGGTCCGA CTCCAGCACC CCACTCAGCA CTCACGCCCC GGCTCCCCCG 
GCAAGCTTTC CCACCAAGAA ACCTCCCAGC CCCGAGCCCA AGCACAGCGA GCTCCTGGTC 
AGTGTCGGCA GCATCACCTC CGACCACGCC GGCACACCGT CTGCTCCCTA CCTGGCCGGC 
CTCGACTCCA GCCGGCTGAG CCCCGAGGTG CAGTCCATGG CCGAGAGCCG GGGATTTGAA 
GCTGATGACG AGAGGAGCGA GCTGGTCAGC GAGGGCCGGC CAGTGGAGAC AGACAGCGAG 
AGCGACTTTC CGGTCTTCCC CGCCGCCCCG GCCTCGGACA GGCTCTTCCG AGGAAAACTC 
CAAGAGGCCG CGAGGACCAG CCGGAGAAAC TCAGAAGGCA GTGAGGTCAG CTGCACGGAA 
GGAAGTTTAA CACCAAGTTT AGAAAGCCGG AGACAGCTCT TCAGCTCCCA TAAACTGATC 
GAGTGTGACA CTCTGTCCAG GAAGAAATCC GCCAGGTTCA AGTCAGACAG TGGGAGTCTA 
GGAGATGCCA AGAATGAGAA AGAAGCCCCT TCCATCACCA AAGTGTTCGA CGTTATGAAA 
AAAGGAAAAT CCACCGGAAA TTTACTGACA CCACCAGCCA GAAGCGAATC GGACAAACAG 
GAACCCACTT GGAAAACAAA AATAGCCGAT CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC 
GATGACATGT TTGGAGTAGG AAGTCAAAAA ACCATCGCCG AACCTGCCAA AAGGAAAAAC 
ATCAAACGCA GACACACGCT GGGTGGGCAC AGGGATGCTA CTGAAATGAG TGTTCTGAAT 
TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC AGAGAATCCG AACTTTCAGC TGTGAATCGA 
TTGAAGCCCA AATGCTCCGC CCAGGACCTG TCCATCTCAG ACTGGCTGGC CAGGGAGCGG 
CTACGCACCA GTATGACTGA CCTGAGCAGA GGGGACACGG GGGACCCCCG GCCTGAGAGT 
CTTGGCACCT TAGAAATACC AACCAGGGAC CCGCCCCTGT CTTTCCAGTC TGATGCAGAC 
AGTTCTTCCA GCACCTTGGC TTCAACTAAC AGGGCCCCTC TTTCCACACC ATCACAGTCA 
CCTGACCAAA TAAATGGAGA AAGCTTCCAG AACACGAGCC AAAACTCCAG TTCTGCAGCC 
AGGGTCCAAC CTCATCAACT GTCTGAAACC CCAGATCACA AAGCACAGTT CCATCCCTGT 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_010822424.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820726.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X6, mRNA.

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CloneID	OBa53931
Clone ID Related Accession (Same CDS sequence)	XM_010822424.2 , XM_010811197.2
Accession Version	XM_010822424.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646129.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822424.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGGCGTATT CTCAAGATGC CTACCTGAAA 
GGCAATGAAG CCTACAGCGG CAATGCCCGC AACATCCCTG AGCCCCCACC AGTCTGCTAC 
CCCTGGCCGT CATCCGCCCC TCCCGCCATG GCGCAGCCCC CTGAACCACC TCCTCCAGAC 
TCCACAGCAA GCAAACCGCA GACTAGCCCC CCAGTATTGA CGCAGCCCGG CAGGGCATAC 
AGGATGGAAA TCCAAGTGCC TCCGTCACCA CCAGAGGTGG CCAAGTCCAA CACAGCAGTG 
TGTGTCTGCA ATGAGAGTGT GAGGACCGTC ATTGTACCTT CTGAGAAGGT TGTAGATTTA 
TTACCTAACA GAAGCAACCA CACAGTTCCC GCTCACAGAA CTGAGGAAGT GAGGTATGGC 
CGAAACGAAC AGCCCTCTTT CAAAACAGCG ACGAGAACCA CCTCACCGCC ATCCTCAGTT 
CCCACTGCCC ATCTCATCCA CCAGCCCGGC TCCAGGTCGT TGGAACCTTC TGGCATTTTA 
CTTAAATCTG GCCACTACGG CGGACACTCA GAAGGTCTGG TGAGCAGATC TCCAGCTGTG 
GAGTCTCCTT CCGTAACTGT CAACCACTAC TCTCCCAACT CCCATCAGCA CATAGACTGG 
AAAAACTATA AAACTTACAA AGAGTATATT GATAACCGGC GCTTGCACAT GGGCTGTCGG 
ACCATTCAAG AAAGGTTAGA TAGTTTAAGA GCCGCCTCTC AGAGCACAGC GGATTATAAC 
CAGGTGGTGC TGAACCGCGC TGCCCTGCAG GTACGGCGTC GGAGCACCTC TCAGGACCGA 
GTGCCCCAGT CCATCCAGGT CCGGCAGCGC AGCGTGTCCC AGGAGAGGCT GGAGGACTCT 
GTGTTGATGA TGTATTGTCC GAGGAGTGCC TCTCAAGGTG CGCTGACATC CCCTTCTATT 
AGTTTTAGTA ACCACAGAAC TCGCTCGTGG GATTACATCG AGGGACAGGG TGAAACCTCG 
GAAAACGTCC ATTCTGAATG TCCACCGCCG GATGCAAATG GTGAACGAAA GCAGACTTAC 
AAGTGGAGTG GGTTCACCGA ACAGGACGAT CGTCGAGGTA TTTACGAGAG ACCCAGGCAG 
CAAGAAATTC ACAAATCTTT CCGAGGCTCC AATTTTACAG TGGCTCCCAG TGTGGTGAAT 
TCTGACAGCA GGAGAGTGAG CAGCAGAATC GGGGGATCAG TGTCTCAGTT TAAAAAAATT 
CCAGCAGATC TAAAACCCCT GCAGCCCAGC AGAAATTTTC AAACTGCTTG TGGAATATCC 
CAGCCTCGAG GGATTTCCCA AGACAGGTCA CCTCTTGGGA AAGTCCGAAG TAACTCTCTG 
AAAGGGCCTT CTATGCATGT CGCGAAACCG CCCTCCAGCC AGAACTCATT TCTTTCTATC 
AAAGACCAAA GACCAGTAAA TCACTTGCAT CAAAACAGTT TATTGAATCA GCAGCCATGG 
TTACGGACGG AAAGTGCCCC CGATCACCAA GTGGACACTG GGAAGCCCCC TTCTCTTTCC 
GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA AGTGAGAATG CTAGCACTCC AGATTTTGAG 
TTATCCGTCT CTCAGAGGAA TCAAGATTTA AATGTACAAG AGGTGGAAAT TCAGCAATCG 
AATGCTGTAG ATAATAAAGA AACTGTCATC CTAAGAGAAA AACCTCCGTC TGGTCGCCAG 
ACACCGCAGC CTTTAAGGCA TCAGTCTTAC ATCTTAGCAG TGAGTGACCA GGAGACAGGC 
TCAGACACCA CCTGCTGGCT GCCTAATGAT GCTCGCCGGG AGGTCCATAT AAAAAGGATG 
GAGGAGAGGA AGGCCTCAAG TACCACTCCA CCTGGTGATT CCTTGGCTTC CATCCCATTT 
ATAGATGAAC CTACCAGCCC TAGCATCGAT CACGACATTT CTCACATCCC CGCCTCTGCG 
GTCATCTCTG CCTCCACCTC CCAGGTACCC TCCATAGCAA CGGTCCCTCC CAGCCTCACA 
ACTTCAGCTC CATTAATCCG ACGTCAGCTC TCCCATGACC AGGAATCTGT TGGACCTCCC 
AGCCTGGATG CCCAGCCCAG CTCAAAGACA GAGCGATCCA AATCATATGA TGAGGGCCTG 
GATGATTACA GAGAAGATGC AAAACTGTCT TTTAAACATG TATCTAGCCT GAAGGGAATC 
AAGATCTTAG ACAGTCAAAA GTCATCAGAA GATTCCGGAT CCAGGAAAGA CTCTTCCTCA 
GAGGTTTTCA GCGATGCTGC CAAGGAAGGG TGGCTTCATT TCAGGCCACT CGTCACTGAT 
AAGGGCAAGC GCGTTGGTGG AAGTATTCGG CCATGGAAGC AGATGTATGT TGTACTTCGG 
GGCCATTCGC TGTATCTGTA CAAAGATAAA CGAGAACAGA CGACTCCGTC TGAGGAGGAG 
CAGCCCATCA GTGTTAACGC CTGCTTAATA GACATCTCTT ACAGCGAGAC CAAGAGGAAG 
AATGTCTTTC GACTCACCAC GTCTGACTGC GAGTGCCTGT TTCAGGCTGA AGACAGGGAT 
GACATGTTAG CCTGGATCAA AACCATCCAG GAGAGCAGCA ATTTAAACGA GGAGGACACT 
GGAGTCACTA ACAGGGACCT AATTAGTCGA AGAATAAAAG AATACAACAG TCTAATGAGC 
AGCAAAGCAG AGCCATTGCC GAAGACCCCT CGCCAGAGTC TCAGCATCCG GCAGACCCTA 
CTTGGTGCTA AAGCAGAGCC TCGGACTCAG AGTCCACACT CTCCCAAAGA GGAGTCAGAA 
AGGAAGCTTC TTAGTAAAGA TGATACCAGT CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA 
GGCATTCCAA GTATTATGAG AAAGACATTT GAGAAAAAGC CTACTGCTAC GGGAACATTC 
GGGGTCAGAC TGGACGACTG CCCACCAGCT CATACAAACC GGTATATTCC ATTAATAGTT 
GACATATGTT GTAAATTAGT TGAAGAAAGA GGTCTGGAAT ATACAGGTAT TTACAGAGTC 
CCTGGAAACA ATGCAGCCAT CTCCAGTATG CAGGAGGAGC TCAACAAGGG AATGGCTGAT 
ATTGATATAC AAGATGATAA ATGGCGAGAT TTGAATGTGA TCAGCAGTTT ATTAAAATCC 
TTCTTCAGAA AACTCCCAGA ACCTCTCTTC ACGAATGATA AATACGCCGA CTTTATTGAA 
GCCAATCGTA AGGAAGATCC TCTAGATCGT CTGAAAACAT TAAAAAGACT AATTCATGAT 
TTGCCCGAAC ATCATTATGA AACACTCAAG TTTCTTTCGG CTCATCTGAA GACAGTAGCA 
GAAAATTCAG AAAAAAATAA GATGGAACCA AGAAACCTAG CCATAGTGTT TGGTCCAACT 
CTTGTGAGAA CCTCCGAAGA TAACATGACA CACATGGTCA CTCACATGCC AGATCAGTAC 
AAGATCGTAG AGACACTCAT CCAGCACCAT GACTGGTTTT TCACAGAAGA AGGTGCTGAA 
GAACCTCTTA CAACAGTGCA GGAGGAAAAC ACAGTAGACT CCCAGCCAGT GCCAAACATA 
GATCATTTAC TCACCAACAT TGGAAGGACA GGCGTCTCCC CTGGAGATGT ATCAGATTCA 
GCTACTAGTG ACTCAACAAA ATCTAAGGGT TCTTGGGGAT CCGGAAAAGA CCAGTACAGC 
AAGGACCTGC TTGTATCCTC CATCTTCGCG GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA 
GAAAAAGCAC AACCCAGCAG CTCGGAAGAC GAGCTGGACA ATGTGTTTTT CAAGAAAGAA 
AACTCAGAGC AGGGTCACCA CGACATTAAA GAAGAGTCCA AAAAAGAGAG TGAGACACCA 
GGCCGGAAAC AAAGGACCGT TGCTCCCAAA GAAAACAACA CGAAGAAAGA CAGCAGCGGA 
GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA 
AGCACCAAGC AGAGCCGGTC ACCCACCCTG AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC 
CCCAGGGCCC TCGCAGCCCA GAAGACCTCC CACCTGGAAG ACACGGGGTC CGACTCCAGC 
ACCCCACTCA GCACTCACGC CCCGGCTCCC CCGGCAAGCT TTCCCACCAA GAAACCTCCC 
AGCCCCGAGC CCAAGCACAG CGAGCTCCTG GTCAGTGTCG GCAGCATCAC CTCCGACCAC 
GCCGGCACAC CGTCTGCTCC CTACCTGGCC GGCCTCGACT CCAGCCGGCT GAGCCCCGAG 
GTGCAGTCCA TGGCCGAGAG CCGGGGATTT GAAGCTGATG ACGAGAGGAG CGAGCTGGTC 
AGCGAGGGCC GGCCAGTGGA GACAGACAGC GAGAGCGACT TTCCGGTCTT CCCCGCCGCC 
CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA 
AACTCAGAAG GCAGTGAGGT CAGCTGCACG GAAGGAAGTT TAACACCAAG TTTAGAAAGC 
CGGAGACAGC TCTTCAGCTC CCATAAACTG ATCGAGTGTG ACACTCTGTC CAGGAAGAAA 
TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT CTAGGAGATG CCAAGAATGA GAAAGAAGCC 
CCTTCCATCA CCAAAGTGTT CGACGTTATG AAAAAAGGAA AATCCACCGG AAATTTACTG 
ACACCACCAG CCAGAAGCGA ATCGGACAAA CAGGAACCCA CTTGGAAAAC AAAAATAGCC 
GATCGGTTAA AACTGAGGCC CAAAGCCCCG GCCGATGACA TGTTTGGAGT AGGAAGTCAA 
AAAACCATCG CCGAACCTGC CAAAAGGAAA AACATCAAAC GCAGACACAC GCTGGGTGGG 
CACAGGGATG CTACTGAAAT GAGTGTTCTG AATTTCTGGA AAGCCCAGGA GCACAGCGGG 
GACAGAGAAT CCGAACTTTC AGCTGTGAAT CGATTGAAGC CCAAATGCTC CGCCCAGGAC 
CTGTCCATCT CAGACTGGCT GGCCAGGGAG CGGCTACGCA CCAGTATGAC TGACCTGAGC 
AGAGGGGACA CGGGGGACCC CCGGCCTGAG AGTCTTGGCA CCTTAGAAAT ACCAACCAGG 
GACCCGCCCC TGTCTTTCCA GTCTGATGCA GACAGTTCTT CCAGCACCTT GGCTTCAACT 
AACAGGGCCC CTCTTTCCAC ACCATCACAG TCACCTGACC AAATAAATGG AGAAAGCTTC 
CAGAACACGA GCCAAAACTC CAGTTCTGCA GCCAGGGTCC AACCTCATCA ACTGTCTGAA 
ACCCCAGATC ACAAAGCACA GTTCCATCCC TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820726.1
CDS	539..6397
Translation	MMATRRTGLPEGDGDTKLKACGSPTCEVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_010822424.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822424.2

ORF Insert Sequence:

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGGCGTATT CTCAAGATGC CTACCTGAAA 
GGCAATGAAG CCTACAGCGG CAATGCCCGC AACATCCCTG AGCCCCCACC AGTCTGCTAC 
CCCTGGCCGT CATCCGCCCC TCCCGCCATG GCGCAGCCCC CTGAACCACC TCCTCCAGAC 
TCCACAGCAA GCAAACCGCA GACTAGCCCC CCAGTATTGA CGCAGCCCGG CAGGGCATAC 
AGGATGGAAA TCCAAGTGCC TCCGTCACCA CCAGAGGTGG CCAAGTCCAA CACAGCAGTG 
TGTGTCTGCA ATGAGAGTGT GAGGACCGTC ATTGTACCTT CTGAGAAGGT TGTAGATTTA 
TTACCTAACA GAAGCAACCA CACAGTTCCC GCTCACAGAA CTGAGGAAGT GAGGTATGGC 
CGAAACGAAC AGCCCTCTTT CAAAACAGCG ACGAGAACCA CCTCACCGCC ATCCTCAGTT 
CCCACTGCCC ATCTCATCCA CCAGCCCGGC TCCAGGTCGT TGGAACCTTC TGGCATTTTA 
CTTAAATCTG GCCACTACGG CGGACACTCA GAAGGTCTGG TGAGCAGATC TCCAGCTGTG 
GAGTCTCCTT CCGTAACTGT CAACCACTAC TCTCCCAACT CCCATCAGCA CATAGACTGG 
AAAAACTATA AAACTTACAA AGAGTATATT GATAACCGGC GCTTGCACAT GGGCTGTCGG 
ACCATTCAAG AAAGGTTAGA TAGTTTAAGA GCCGCCTCTC AGAGCACAGC GGATTATAAC 
CAGGTGGTGC TGAACCGCGC TGCCCTGCAG GTACGGCGTC GGAGCACCTC TCAGGACCGA 
GTGCCCCAGT CCATCCAGGT CCGGCAGCGC AGCGTGTCCC AGGAGAGGCT GGAGGACTCT 
GTGTTGATGA TGTATTGTCC GAGGAGTGCC TCTCAAGGTG CGCTGACATC CCCTTCTATT 
AGTTTTAGTA ACCACAGAAC TCGCTCGTGG GATTACATCG AGGGACAGGG TGAAACCTCG 
GAAAACGTCC ATTCTGAATG TCCACCGCCG GATGCAAATG GTGAACGAAA GCAGACTTAC 
AAGTGGAGTG GGTTCACCGA ACAGGACGAT CGTCGAGGTA TTTACGAGAG ACCCAGGCAG 
CAAGAAATTC ACAAATCTTT CCGAGGCTCC AATTTTACAG TGGCTCCCAG TGTGGTGAAT 
TCTGACAGCA GGAGAGTGAG CAGCAGAATC GGGGGATCAG TGTCTCAGTT TAAAAAAATT 
CCAGCAGATC TAAAACCCCT GCAGCCCAGC AGAAATTTTC AAACTGCTTG TGGAATATCC 
CAGCCTCGAG GGATTTCCCA AGACAGGTCA CCTCTTGGGA AAGTCCGAAG TAACTCTCTG 
AAAGGGCCTT CTATGCATGT CGCGAAACCG CCCTCCAGCC AGAACTCATT TCTTTCTATC 
AAAGACCAAA GACCAGTAAA TCACTTGCAT CAAAACAGTT TATTGAATCA GCAGCCATGG 
TTACGGACGG AAAGTGCCCC CGATCACCAA GTGGACACTG GGAAGCCCCC TTCTCTTTCC 
GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA AGTGAGAATG CTAGCACTCC AGATTTTGAG 
TTATCCGTCT CTCAGAGGAA TCAAGATTTA AATGTACAAG AGGTGGAAAT TCAGCAATCG 
AATGCTGTAG ATAATAAAGA AACTGTCATC CTAAGAGAAA AACCTCCGTC TGGTCGCCAG 
ACACCGCAGC CTTTAAGGCA TCAGTCTTAC ATCTTAGCAG TGAGTGACCA GGAGACAGGC 
TCAGACACCA CCTGCTGGCT GCCTAATGAT GCTCGCCGGG AGGTCCATAT AAAAAGGATG 
GAGGAGAGGA AGGCCTCAAG TACCACTCCA CCTGGTGATT CCTTGGCTTC CATCCCATTT 
ATAGATGAAC CTACCAGCCC TAGCATCGAT CACGACATTT CTCACATCCC CGCCTCTGCG 
GTCATCTCTG CCTCCACCTC CCAGGTACCC TCCATAGCAA CGGTCCCTCC CAGCCTCACA 
ACTTCAGCTC CATTAATCCG ACGTCAGCTC TCCCATGACC AGGAATCTGT TGGACCTCCC 
AGCCTGGATG CCCAGCCCAG CTCAAAGACA GAGCGATCCA AATCATATGA TGAGGGCCTG 
GATGATTACA GAGAAGATGC AAAACTGTCT TTTAAACATG TATCTAGCCT GAAGGGAATC 
AAGATCTTAG ACAGTCAAAA GTCATCAGAA GATTCCGGAT CCAGGAAAGA CTCTTCCTCA 
GAGGTTTTCA GCGATGCTGC CAAGGAAGGG TGGCTTCATT TCAGGCCACT CGTCACTGAT 
AAGGGCAAGC GCGTTGGTGG AAGTATTCGG CCATGGAAGC AGATGTATGT TGTACTTCGG 
GGCCATTCGC TGTATCTGTA CAAAGATAAA CGAGAACAGA CGACTCCGTC TGAGGAGGAG 
CAGCCCATCA GTGTTAACGC CTGCTTAATA GACATCTCTT ACAGCGAGAC CAAGAGGAAG 
AATGTCTTTC GACTCACCAC GTCTGACTGC GAGTGCCTGT TTCAGGCTGA AGACAGGGAT 
GACATGTTAG CCTGGATCAA AACCATCCAG GAGAGCAGCA ATTTAAACGA GGAGGACACT 
GGAGTCACTA ACAGGGACCT AATTAGTCGA AGAATAAAAG AATACAACAG TCTAATGAGC 
AGCAAAGCAG AGCCATTGCC GAAGACCCCT CGCCAGAGTC TCAGCATCCG GCAGACCCTA 
CTTGGTGCTA AAGCAGAGCC TCGGACTCAG AGTCCACACT CTCCCAAAGA GGAGTCAGAA 
AGGAAGCTTC TTAGTAAAGA TGATACCAGT CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA 
GGCATTCCAA GTATTATGAG AAAGACATTT GAGAAAAAGC CTACTGCTAC GGGAACATTC 
GGGGTCAGAC TGGACGACTG CCCACCAGCT CATACAAACC GGTATATTCC ATTAATAGTT 
GACATATGTT GTAAATTAGT TGAAGAAAGA GGTCTGGAAT ATACAGGTAT TTACAGAGTC 
CCTGGAAACA ATGCAGCCAT CTCCAGTATG CAGGAGGAGC TCAACAAGGG AATGGCTGAT 
ATTGATATAC AAGATGATAA ATGGCGAGAT TTGAATGTGA TCAGCAGTTT ATTAAAATCC 
TTCTTCAGAA AACTCCCAGA ACCTCTCTTC ACGAATGATA AATACGCCGA CTTTATTGAA 
GCCAATCGTA AGGAAGATCC TCTAGATCGT CTGAAAACAT TAAAAAGACT AATTCATGAT 
TTGCCCGAAC ATCATTATGA AACACTCAAG TTTCTTTCGG CTCATCTGAA GACAGTAGCA 
GAAAATTCAG AAAAAAATAA GATGGAACCA AGAAACCTAG CCATAGTGTT TGGTCCAACT 
CTTGTGAGAA CCTCCGAAGA TAACATGACA CACATGGTCA CTCACATGCC AGATCAGTAC 
AAGATCGTAG AGACACTCAT CCAGCACCAT GACTGGTTTT TCACAGAAGA AGGTGCTGAA 
GAACCTCTTA CAACAGTGCA GGAGGAAAAC ACAGTAGACT CCCAGCCAGT GCCAAACATA 
GATCATTTAC TCACCAACAT TGGAAGGACA GGCGTCTCCC CTGGAGATGT ATCAGATTCA 
GCTACTAGTG ACTCAACAAA ATCTAAGGGT TCTTGGGGAT CCGGAAAAGA CCAGTACAGC 
AAGGACCTGC TTGTATCCTC CATCTTCGCG GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA 
GAAAAAGCAC AACCCAGCAG CTCGGAAGAC GAGCTGGACA ATGTGTTTTT CAAGAAAGAA 
AACTCAGAGC AGGGTCACCA CGACATTAAA GAAGAGTCCA AAAAAGAGAG TGAGACACCA 
GGCCGGAAAC AAAGGACCGT TGCTCCCAAA GAAAACAACA CGAAGAAAGA CAGCAGCGGA 
GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA 
AGCACCAAGC AGAGCCGGTC ACCCACCCTG AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC 
CCCAGGGCCC TCGCAGCCCA GAAGACCTCC CACCTGGAAG ACACGGGGTC CGACTCCAGC 
ACCCCACTCA GCACTCACGC CCCGGCTCCC CCGGCAAGCT TTCCCACCAA GAAACCTCCC 
AGCCCCGAGC CCAAGCACAG CGAGCTCCTG GTCAGTGTCG GCAGCATCAC CTCCGACCAC 
GCCGGCACAC CGTCTGCTCC CTACCTGGCC GGCCTCGACT CCAGCCGGCT GAGCCCCGAG 
GTGCAGTCCA TGGCCGAGAG CCGGGGATTT GAAGCTGATG ACGAGAGGAG CGAGCTGGTC 
AGCGAGGGCC GGCCAGTGGA GACAGACAGC GAGAGCGACT TTCCGGTCTT CCCCGCCGCC 
CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA 
AACTCAGAAG GCAGTGAGGT CAGCTGCACG GAAGGAAGTT TAACACCAAG TTTAGAAAGC 
CGGAGACAGC TCTTCAGCTC CCATAAACTG ATCGAGTGTG ACACTCTGTC CAGGAAGAAA 
TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT CTAGGAGATG CCAAGAATGA GAAAGAAGCC 
CCTTCCATCA CCAAAGTGTT CGACGTTATG AAAAAAGGAA AATCCACCGG AAATTTACTG 
ACACCACCAG CCAGAAGCGA ATCGGACAAA CAGGAACCCA CTTGGAAAAC AAAAATAGCC 
GATCGGTTAA AACTGAGGCC CAAAGCCCCG GCCGATGACA TGTTTGGAGT AGGAAGTCAA 
AAAACCATCG CCGAACCTGC CAAAAGGAAA AACATCAAAC GCAGACACAC GCTGGGTGGG 
CACAGGGATG CTACTGAAAT GAGTGTTCTG AATTTCTGGA AAGCCCAGGA GCACAGCGGG 
GACAGAGAAT CCGAACTTTC AGCTGTGAAT CGATTGAAGC CCAAATGCTC CGCCCAGGAC 
CTGTCCATCT CAGACTGGCT GGCCAGGGAG CGGCTACGCA CCAGTATGAC TGACCTGAGC 
AGAGGGGACA CGGGGGACCC CCGGCCTGAG AGTCTTGGCA CCTTAGAAAT ACCAACCAGG 
GACCCGCCCC TGTCTTTCCA GTCTGATGCA GACAGTTCTT CCAGCACCTT GGCTTCAACT 
AACAGGGCCC CTCTTTCCAC ACCATCACAG TCACCTGACC AAATAAATGG AGAAAGCTTC 
CAGAACACGA GCCAAAACTC CAGTTCTGCA GCCAGGGTCC AACCTCATCA ACTGTCTGAA 
ACCCCAGATC ACAAAGCACA GTTCCATCCC TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_010811197.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809499.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X6, mRNA.

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CloneID	OBa53931
Clone ID Related Accession (Same CDS sequence)	XM_010822424.2 , XM_010811197.2
Accession Version	XM_010811197.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104365.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGGCGTATT CTCAAGATGC CTACCTGAAA 
GGCAATGAAG CCTACAGCGG CAATGCCCGC AACATCCCTG AGCCCCCACC AGTCTGCTAC 
CCCTGGCCGT CATCCGCCCC TCCCGCCATG GCGCAGCCCC CTGAACCACC TCCTCCAGAC 
TCCACAGCAA GCAAACCGCA GACTAGCCCC CCAGTATTGA CGCAGCCCGG CAGGGCATAC 
AGGATGGAAA TCCAAGTGCC TCCGTCACCA CCAGAGGTGG CCAAGTCCAA CACAGCAGTG 
TGTGTCTGCA ATGAGAGTGT GAGGACCGTC ATTGTACCTT CTGAGAAGGT TGTAGATTTA 
TTACCTAACA GAAGCAACCA CACAGTTCCC GCTCACAGAA CTGAGGAAGT GAGGTATGGC 
CGAAACGAAC AGCCCTCTTT CAAAACAGCG ACGAGAACCA CCTCACCGCC ATCCTCAGTT 
CCCACTGCCC ATCTCATCCA CCAGCCCGGC TCCAGGTCGT TGGAACCTTC TGGCATTTTA 
CTTAAATCTG GCCACTACGG CGGACACTCA GAAGGTCTGG TGAGCAGATC TCCAGCTGTG 
GAGTCTCCTT CCGTAACTGT CAACCACTAC TCTCCCAACT CCCATCAGCA CATAGACTGG 
AAAAACTATA AAACTTACAA AGAGTATATT GATAACCGGC GCTTGCACAT GGGCTGTCGG 
ACCATTCAAG AAAGGTTAGA TAGTTTAAGA GCCGCCTCTC AGAGCACAGC GGATTATAAC 
CAGGTGGTGC TGAACCGCGC TGCCCTGCAG GTACGGCGTC GGAGCACCTC TCAGGACCGA 
GTGCCCCAGT CCATCCAGGT CCGGCAGCGC AGCGTGTCCC AGGAGAGGCT GGAGGACTCT 
GTGTTGATGA TGTATTGTCC GAGGAGTGCC TCTCAAGGTG CGCTGACATC CCCTTCTATT 
AGTTTTAGTA ACCACAGAAC TCGCTCGTGG GATTACATCG AGGGACAGGG TGAAACCTCG 
GAAAACGTCC ATTCTGAATG TCCACCGCCG GATGCAAATG GTGAACGAAA GCAGACTTAC 
AAGTGGAGTG GGTTCACCGA ACAGGACGAT CGTCGAGGTA TTTACGAGAG ACCCAGGCAG 
CAAGAAATTC ACAAATCTTT CCGAGGCTCC AATTTTACAG TGGCTCCCAG TGTGGTGAAT 
TCTGACAGCA GGAGAGTGAG CAGCAGAATC GGGGGATCAG TGTCTCAGTT TAAAAAAATT 
CCAGCAGATC TAAAACCCCT GCAGCCCAGC AGAAATTTTC AAACTGCTTG TGGAATATCC 
CAGCCTCGAG GGATTTCCCA AGACAGGTCA CCTCTTGGGA AAGTCCGAAG TAACTCTCTG 
AAAGGGCCTT CTATGCATGT CGCGAAACCG CCCTCCAGCC AGAACTCATT TCTTTCTATC 
AAAGACCAAA GACCAGTAAA TCACTTGCAT CAAAACAGTT TATTGAATCA GCAGCCATGG 
TTACGGACGG AAAGTGCCCC CGATCACCAA GTGGACACTG GGAAGCCCCC TTCTCTTTCC 
GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA AGTGAGAATG CTAGCACTCC AGATTTTGAG 
TTATCCGTCT CTCAGAGGAA TCAAGATTTA AATGTACAAG AGGTGGAAAT TCAGCAATCG 
AATGCTGTAG ATAATAAAGA AACTGTCATC CTAAGAGAAA AACCTCCGTC TGGTCGCCAG 
ACACCGCAGC CTTTAAGGCA TCAGTCTTAC ATCTTAGCAG TGAGTGACCA GGAGACAGGC 
TCAGACACCA CCTGCTGGCT GCCTAATGAT GCTCGCCGGG AGGTCCATAT AAAAAGGATG 
GAGGAGAGGA AGGCCTCAAG TACCACTCCA CCTGGTGATT CCTTGGCTTC CATCCCATTT 
ATAGATGAAC CTACCAGCCC TAGCATCGAT CACGACATTT CTCACATCCC CGCCTCTGCG 
GTCATCTCTG CCTCCACCTC CCAGGTACCC TCCATAGCAA CGGTCCCTCC CAGCCTCACA 
ACTTCAGCTC CATTAATCCG ACGTCAGCTC TCCCATGACC AGGAATCTGT TGGACCTCCC 
AGCCTGGATG CCCAGCCCAG CTCAAAGACA GAGCGATCCA AATCATATGA TGAGGGCCTG 
GATGATTACA GAGAAGATGC AAAACTGTCT TTTAAACATG TATCTAGCCT GAAGGGAATC 
AAGATCTTAG ACAGTCAAAA GTCATCAGAA GATTCCGGAT CCAGGAAAGA CTCTTCCTCA 
GAGGTTTTCA GCGATGCTGC CAAGGAAGGG TGGCTTCATT TCAGGCCACT CGTCACTGAT 
AAGGGCAAGC GCGTTGGTGG AAGTATTCGG CCATGGAAGC AGATGTATGT TGTACTTCGG 
GGCCATTCGC TGTATCTGTA CAAAGATAAA CGAGAACAGA CGACTCCGTC TGAGGAGGAG 
CAGCCCATCA GTGTTAACGC CTGCTTAATA GACATCTCTT ACAGCGAGAC CAAGAGGAAG 
AATGTCTTTC GACTCACCAC GTCTGACTGC GAGTGCCTGT TTCAGGCTGA AGACAGGGAT 
GACATGTTAG CCTGGATCAA AACCATCCAG GAGAGCAGCA ATTTAAACGA GGAGGACACT 
GGAGTCACTA ACAGGGACCT AATTAGTCGA AGAATAAAAG AATACAACAG TCTAATGAGC 
AGCAAAGCAG AGCCATTGCC GAAGACCCCT CGCCAGAGTC TCAGCATCCG GCAGACCCTA 
CTTGGTGCTA AAGCAGAGCC TCGGACTCAG AGTCCACACT CTCCCAAAGA GGAGTCAGAA 
AGGAAGCTTC TTAGTAAAGA TGATACCAGT CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA 
GGCATTCCAA GTATTATGAG AAAGACATTT GAGAAAAAGC CTACTGCTAC GGGAACATTC 
GGGGTCAGAC TGGACGACTG CCCACCAGCT CATACAAACC GGTATATTCC ATTAATAGTT 
GACATATGTT GTAAATTAGT TGAAGAAAGA GGTCTGGAAT ATACAGGTAT TTACAGAGTC 
CCTGGAAACA ATGCAGCCAT CTCCAGTATG CAGGAGGAGC TCAACAAGGG AATGGCTGAT 
ATTGATATAC AAGATGATAA ATGGCGAGAT TTGAATGTGA TCAGCAGTTT ATTAAAATCC 
TTCTTCAGAA AACTCCCAGA ACCTCTCTTC ACGAATGATA AATACGCCGA CTTTATTGAA 
GCCAATCGTA AGGAAGATCC TCTAGATCGT CTGAAAACAT TAAAAAGACT AATTCATGAT 
TTGCCCGAAC ATCATTATGA AACACTCAAG TTTCTTTCGG CTCATCTGAA GACAGTAGCA 
GAAAATTCAG AAAAAAATAA GATGGAACCA AGAAACCTAG CCATAGTGTT TGGTCCAACT 
CTTGTGAGAA CCTCCGAAGA TAACATGACA CACATGGTCA CTCACATGCC AGATCAGTAC 
AAGATCGTAG AGACACTCAT CCAGCACCAT GACTGGTTTT TCACAGAAGA AGGTGCTGAA 
GAACCTCTTA CAACAGTGCA GGAGGAAAAC ACAGTAGACT CCCAGCCAGT GCCAAACATA 
GATCATTTAC TCACCAACAT TGGAAGGACA GGCGTCTCCC CTGGAGATGT ATCAGATTCA 
GCTACTAGTG ACTCAACAAA ATCTAAGGGT TCTTGGGGAT CCGGAAAAGA CCAGTACAGC 
AAGGACCTGC TTGTATCCTC CATCTTCGCG GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA 
GAAAAAGCAC AACCCAGCAG CTCGGAAGAC GAGCTGGACA ATGTGTTTTT CAAGAAAGAA 
AACTCAGAGC AGGGTCACCA CGACATTAAA GAAGAGTCCA AAAAAGAGAG TGAGACACCA 
GGCCGGAAAC AAAGGACCGT TGCTCCCAAA GAAAACAACA CGAAGAAAGA CAGCAGCGGA 
GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA 
AGCACCAAGC AGAGCCGGTC ACCCACCCTG AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC 
CCCAGGGCCC TCGCAGCCCA GAAGACCTCC CACCTGGAAG ACACGGGGTC CGACTCCAGC 
ACCCCACTCA GCACTCACGC CCCGGCTCCC CCGGCAAGCT TTCCCACCAA GAAACCTCCC 
AGCCCCGAGC CCAAGCACAG CGAGCTCCTG GTCAGTGTCG GCAGCATCAC CTCCGACCAC 
GCCGGCACAC CGTCTGCTCC CTACCTGGCC GGCCTCGACT CCAGCCGGCT GAGCCCCGAG 
GTGCAGTCCA TGGCCGAGAG CCGGGGATTT GAAGCTGATG ACGAGAGGAG CGAGCTGGTC 
AGCGAGGGCC GGCCAGTGGA GACAGACAGC GAGAGCGACT TTCCGGTCTT CCCCGCCGCC 
CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA 
AACTCAGAAG GCAGTGAGGT CAGCTGCACG GAAGGAAGTT TAACACCAAG TTTAGAAAGC 
CGGAGACAGC TCTTCAGCTC CCATAAACTG ATCGAGTGTG ACACTCTGTC CAGGAAGAAA 
TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT CTAGGAGATG CCAAGAATGA GAAAGAAGCC 
CCTTCCATCA CCAAAGTGTT CGACGTTATG AAAAAAGGAA AATCCACCGG AAATTTACTG 
ACACCACCAG CCAGAAGCGA ATCGGACAAA CAGGAACCCA CTTGGAAAAC AAAAATAGCC 
GATCGGTTAA AACTGAGGCC CAAAGCCCCG GCCGATGACA TGTTTGGAGT AGGAAGTCAA 
AAAACCATCG CCGAACCTGC CAAAAGGAAA AACATCAAAC GCAGACACAC GCTGGGTGGG 
CACAGGGATG CTACTGAAAT GAGTGTTCTG AATTTCTGGA AAGCCCAGGA GCACAGCGGG 
GACAGAGAAT CCGAACTTTC AGCTGTGAAT CGATTGAAGC CCAAATGCTC CGCCCAGGAC 
CTGTCCATCT CAGACTGGCT GGCCAGGGAG CGGCTACGCA CCAGTATGAC TGACCTGAGC 
AGAGGGGACA CGGGGGACCC CCGGCCTGAG AGTCTTGGCA CCTTAGAAAT ACCAACCAGG 
GACCCGCCCC TGTCTTTCCA GTCTGATGCA GACAGTTCTT CCAGCACCTT GGCTTCAACT 
AACAGGGCCC CTCTTTCCAC ACCATCACAG TCACCTGACC AAATAAATGG AGAAAGCTTC 
CAGAACACGA GCCAAAACTC CAGTTCTGCA GCCAGGGTCC AACCTCATCA ACTGTCTGAA 
ACCCCAGATC ACAAAGCACA GTTCCATCCC TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809499.1
CDS	539..6397
Translation	MMATRRTGLPEGDGDTKLKACGSPTCEVSKSKDGKEQSETVSPSEDEMFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSFKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_010811197.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811197.2

ORF Insert Sequence:

ATGATGGCCA CACGTCGGAC TGGTCTGCCT GAGGGAGATG GTGACACCAA GCTCAAGGCC 
TGCGGGTCCC CCACCTGTGA GGTATCAAAA AGTAAAGATG GAAAAGAACA AAGTGAAACT 
GTATCACCAT CCGAAGATGA AATGTTCTCC TGGCCAGGTC CCAAAACAGT TATGTTGAAA 
AGAACATCTC AAGGCTTTGG TTTTACATTG AGGCATTTTA TTGTTTATCC CCCAGAGTCT 
GCAATTCAGT TTTCATTTAA GGATGAAGAA AATGGCAACA GAGGAGGAAA ACAAAGAAAC 
CGTTTGGAAC CCATGGACAC CATCTTTGTT AAACAGGTGA AAGAGGGCGG ACCTGCTTTT 
GAAGCTGGAT TATGCACAGG TGATCGAATT ATAAAAGTCA ATGGAGAAAG TGTTATTGGA 
AAGACCTATT CTCAAGTAAT TGCTTTAATC CAGAACAGTG ATACAACATT GGAACTTAGT 
GTTATGCCAA AAGATGAAGA CATTCTCCAA GTGGCGTATT CTCAAGATGC CTACCTGAAA 
GGCAATGAAG CCTACAGCGG CAATGCCCGC AACATCCCTG AGCCCCCACC AGTCTGCTAC 
CCCTGGCCGT CATCCGCCCC TCCCGCCATG GCGCAGCCCC CTGAACCACC TCCTCCAGAC 
TCCACAGCAA GCAAACCGCA GACTAGCCCC CCAGTATTGA CGCAGCCCGG CAGGGCATAC 
AGGATGGAAA TCCAAGTGCC TCCGTCACCA CCAGAGGTGG CCAAGTCCAA CACAGCAGTG 
TGTGTCTGCA ATGAGAGTGT GAGGACCGTC ATTGTACCTT CTGAGAAGGT TGTAGATTTA 
TTACCTAACA GAAGCAACCA CACAGTTCCC GCTCACAGAA CTGAGGAAGT GAGGTATGGC 
CGAAACGAAC AGCCCTCTTT CAAAACAGCG ACGAGAACCA CCTCACCGCC ATCCTCAGTT 
CCCACTGCCC ATCTCATCCA CCAGCCCGGC TCCAGGTCGT TGGAACCTTC TGGCATTTTA 
CTTAAATCTG GCCACTACGG CGGACACTCA GAAGGTCTGG TGAGCAGATC TCCAGCTGTG 
GAGTCTCCTT CCGTAACTGT CAACCACTAC TCTCCCAACT CCCATCAGCA CATAGACTGG 
AAAAACTATA AAACTTACAA AGAGTATATT GATAACCGGC GCTTGCACAT GGGCTGTCGG 
ACCATTCAAG AAAGGTTAGA TAGTTTAAGA GCCGCCTCTC AGAGCACAGC GGATTATAAC 
CAGGTGGTGC TGAACCGCGC TGCCCTGCAG GTACGGCGTC GGAGCACCTC TCAGGACCGA 
GTGCCCCAGT CCATCCAGGT CCGGCAGCGC AGCGTGTCCC AGGAGAGGCT GGAGGACTCT 
GTGTTGATGA TGTATTGTCC GAGGAGTGCC TCTCAAGGTG CGCTGACATC CCCTTCTATT 
AGTTTTAGTA ACCACAGAAC TCGCTCGTGG GATTACATCG AGGGACAGGG TGAAACCTCG 
GAAAACGTCC ATTCTGAATG TCCACCGCCG GATGCAAATG GTGAACGAAA GCAGACTTAC 
AAGTGGAGTG GGTTCACCGA ACAGGACGAT CGTCGAGGTA TTTACGAGAG ACCCAGGCAG 
CAAGAAATTC ACAAATCTTT CCGAGGCTCC AATTTTACAG TGGCTCCCAG TGTGGTGAAT 
TCTGACAGCA GGAGAGTGAG CAGCAGAATC GGGGGATCAG TGTCTCAGTT TAAAAAAATT 
CCAGCAGATC TAAAACCCCT GCAGCCCAGC AGAAATTTTC AAACTGCTTG TGGAATATCC 
CAGCCTCGAG GGATTTCCCA AGACAGGTCA CCTCTTGGGA AAGTCCGAAG TAACTCTCTG 
AAAGGGCCTT CTATGCATGT CGCGAAACCG CCCTCCAGCC AGAACTCATT TCTTTCTATC 
AAAGACCAAA GACCAGTAAA TCACTTGCAT CAAAACAGTT TATTGAATCA GCAGCCATGG 
TTACGGACGG AAAGTGCCCC CGATCACCAA GTGGACACTG GGAAGCCCCC TTCTCTTTCC 
GGAGCTTCTG TTAAGCCCCC GGCTCTGGCA AGTGAGAATG CTAGCACTCC AGATTTTGAG 
TTATCCGTCT CTCAGAGGAA TCAAGATTTA AATGTACAAG AGGTGGAAAT TCAGCAATCG 
AATGCTGTAG ATAATAAAGA AACTGTCATC CTAAGAGAAA AACCTCCGTC TGGTCGCCAG 
ACACCGCAGC CTTTAAGGCA TCAGTCTTAC ATCTTAGCAG TGAGTGACCA GGAGACAGGC 
TCAGACACCA CCTGCTGGCT GCCTAATGAT GCTCGCCGGG AGGTCCATAT AAAAAGGATG 
GAGGAGAGGA AGGCCTCAAG TACCACTCCA CCTGGTGATT CCTTGGCTTC CATCCCATTT 
ATAGATGAAC CTACCAGCCC TAGCATCGAT CACGACATTT CTCACATCCC CGCCTCTGCG 
GTCATCTCTG CCTCCACCTC CCAGGTACCC TCCATAGCAA CGGTCCCTCC CAGCCTCACA 
ACTTCAGCTC CATTAATCCG ACGTCAGCTC TCCCATGACC AGGAATCTGT TGGACCTCCC 
AGCCTGGATG CCCAGCCCAG CTCAAAGACA GAGCGATCCA AATCATATGA TGAGGGCCTG 
GATGATTACA GAGAAGATGC AAAACTGTCT TTTAAACATG TATCTAGCCT GAAGGGAATC 
AAGATCTTAG ACAGTCAAAA GTCATCAGAA GATTCCGGAT CCAGGAAAGA CTCTTCCTCA 
GAGGTTTTCA GCGATGCTGC CAAGGAAGGG TGGCTTCATT TCAGGCCACT CGTCACTGAT 
AAGGGCAAGC GCGTTGGTGG AAGTATTCGG CCATGGAAGC AGATGTATGT TGTACTTCGG 
GGCCATTCGC TGTATCTGTA CAAAGATAAA CGAGAACAGA CGACTCCGTC TGAGGAGGAG 
CAGCCCATCA GTGTTAACGC CTGCTTAATA GACATCTCTT ACAGCGAGAC CAAGAGGAAG 
AATGTCTTTC GACTCACCAC GTCTGACTGC GAGTGCCTGT TTCAGGCTGA AGACAGGGAT 
GACATGTTAG CCTGGATCAA AACCATCCAG GAGAGCAGCA ATTTAAACGA GGAGGACACT 
GGAGTCACTA ACAGGGACCT AATTAGTCGA AGAATAAAAG AATACAACAG TCTAATGAGC 
AGCAAAGCAG AGCCATTGCC GAAGACCCCT CGCCAGAGTC TCAGCATCCG GCAGACCCTA 
CTTGGTGCTA AAGCAGAGCC TCGGACTCAG AGTCCACACT CTCCCAAAGA GGAGTCAGAA 
AGGAAGCTTC TTAGTAAAGA TGATACCAGT CCTCCAAAAG ACAAAGGCAC GTGGAGAAAA 
GGCATTCCAA GTATTATGAG AAAGACATTT GAGAAAAAGC CTACTGCTAC GGGAACATTC 
GGGGTCAGAC TGGACGACTG CCCACCAGCT CATACAAACC GGTATATTCC ATTAATAGTT 
GACATATGTT GTAAATTAGT TGAAGAAAGA GGTCTGGAAT ATACAGGTAT TTACAGAGTC 
CCTGGAAACA ATGCAGCCAT CTCCAGTATG CAGGAGGAGC TCAACAAGGG AATGGCTGAT 
ATTGATATAC AAGATGATAA ATGGCGAGAT TTGAATGTGA TCAGCAGTTT ATTAAAATCC 
TTCTTCAGAA AACTCCCAGA ACCTCTCTTC ACGAATGATA AATACGCCGA CTTTATTGAA 
GCCAATCGTA AGGAAGATCC TCTAGATCGT CTGAAAACAT TAAAAAGACT AATTCATGAT 
TTGCCCGAAC ATCATTATGA AACACTCAAG TTTCTTTCGG CTCATCTGAA GACAGTAGCA 
GAAAATTCAG AAAAAAATAA GATGGAACCA AGAAACCTAG CCATAGTGTT TGGTCCAACT 
CTTGTGAGAA CCTCCGAAGA TAACATGACA CACATGGTCA CTCACATGCC AGATCAGTAC 
AAGATCGTAG AGACACTCAT CCAGCACCAT GACTGGTTTT TCACAGAAGA AGGTGCTGAA 
GAACCTCTTA CAACAGTGCA GGAGGAAAAC ACAGTAGACT CCCAGCCAGT GCCAAACATA 
GATCATTTAC TCACCAACAT TGGAAGGACA GGCGTCTCCC CTGGAGATGT ATCAGATTCA 
GCTACTAGTG ACTCAACAAA ATCTAAGGGT TCTTGGGGAT CCGGAAAAGA CCAGTACAGC 
AAGGACCTGC TTGTATCCTC CATCTTCGCG GCTGCCAGTC GCAAGAGGAA AAAGCCAAAA 
GAAAAAGCAC AACCCAGCAG CTCGGAAGAC GAGCTGGACA ATGTGTTTTT CAAGAAAGAA 
AACTCAGAGC AGGGTCACCA CGACATTAAA GAAGAGTCCA AAAAAGAGAG TGAGACACCA 
GGCCGGAAAC AAAGGACCGT TGCTCCCAAA GAAAACAACA CGAAGAAAGA CAGCAGCGGA 
GGTGGCAGAG CTGAGCAGAG GGCCCCGCGT GGGGAGAGCT TGGAGCCCCC GGCCCCCCAA 
AGCACCAAGC AGAGCCGGTC ACCCACCCTG AGCTGTCGCC TCGCCGTCCT GAGAGAGAGC 
CCCAGGGCCC TCGCAGCCCA GAAGACCTCC CACCTGGAAG ACACGGGGTC CGACTCCAGC 
ACCCCACTCA GCACTCACGC CCCGGCTCCC CCGGCAAGCT TTCCCACCAA GAAACCTCCC 
AGCCCCGAGC CCAAGCACAG CGAGCTCCTG GTCAGTGTCG GCAGCATCAC CTCCGACCAC 
GCCGGCACAC CGTCTGCTCC CTACCTGGCC GGCCTCGACT CCAGCCGGCT GAGCCCCGAG 
GTGCAGTCCA TGGCCGAGAG CCGGGGATTT GAAGCTGATG ACGAGAGGAG CGAGCTGGTC 
AGCGAGGGCC GGCCAGTGGA GACAGACAGC GAGAGCGACT TTCCGGTCTT CCCCGCCGCC 
CCGGCCTCGG ACAGGCTCTT CCGAGGAAAA CTCCAAGAGG CCGCGAGGAC CAGCCGGAGA 
AACTCAGAAG GCAGTGAGGT CAGCTGCACG GAAGGAAGTT TAACACCAAG TTTAGAAAGC 
CGGAGACAGC TCTTCAGCTC CCATAAACTG ATCGAGTGTG ACACTCTGTC CAGGAAGAAA 
TCCGCCAGGT TCAAGTCAGA CAGTGGGAGT CTAGGAGATG CCAAGAATGA GAAAGAAGCC 
CCTTCCATCA CCAAAGTGTT CGACGTTATG AAAAAAGGAA AATCCACCGG AAATTTACTG 
ACACCACCAG CCAGAAGCGA ATCGGACAAA CAGGAACCCA CTTGGAAAAC AAAAATAGCC 
GATCGGTTAA AACTGAGGCC CAAAGCCCCG GCCGATGACA TGTTTGGAGT AGGAAGTCAA 
AAAACCATCG CCGAACCTGC CAAAAGGAAA AACATCAAAC GCAGACACAC GCTGGGTGGG 
CACAGGGATG CTACTGAAAT GAGTGTTCTG AATTTCTGGA AAGCCCAGGA GCACAGCGGG 
GACAGAGAAT CCGAACTTTC AGCTGTGAAT CGATTGAAGC CCAAATGCTC CGCCCAGGAC 
CTGTCCATCT CAGACTGGCT GGCCAGGGAG CGGCTACGCA CCAGTATGAC TGACCTGAGC 
AGAGGGGACA CGGGGGACCC CCGGCCTGAG AGTCTTGGCA CCTTAGAAAT ACCAACCAGG 
GACCCGCCCC TGTCTTTCCA GTCTGATGCA GACAGTTCTT CCAGCACCTT GGCTTCAACT 
AACAGGGCCC CTCTTTCCAC ACCATCACAG TCACCTGACC AAATAAATGG AGAAAGCTTC 
CAGAACACGA GCCAAAACTC CAGTTCTGCA GCCAGGGTCC AACCTCATCA ACTGTCTGAA 
ACCCCAGATC ACAAAGCACA GTTCCATCCC TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_010811198.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809500.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X11, mRNA.

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CloneID	OBa53932
Clone ID Related Accession (Same CDS sequence)	XM_010811198.2 , XM_010822425.2
Accession Version	XM_010811198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAAAG ATGAAGACAT TCTCCAAGTG GCGTATTCTC AAGATGCCTA CCTGAAAGGC 
AATGAAGCCT ACAGCGGCAA TGCCCGCAAC ATCCCTGAGC CCCCACCAGT CTGCTACCCC 
TGGCCGTCAT CCGCCCCTCC CGCCATGGCG CAGCCCCCTG AACCACCTCC TCCAGACTCC 
ACAGCAAGCA AACCGCAGAC TAGCCCCCCA GTATTGACGC AGCCCGGCAG GGCATACAGG 
ATGGAAATCC AAGTGCCTCC GTCACCACCA GAGGTGGCCA AGTCCAACAC AGCAGTGTGT 
GTCTGCAATG AGAGTGTGAG GACCGTCATT GTACCTTCTG AGAAGGTTGT AGATTTATTA 
CCTAACAGAA GCAACCACAC AGTTCCCGCT CACAGAACTG AGGAAGTGAG GTATGGCCGA 
AACGAACAGC CCTCTTTCAA AACAGCGACG AGAACCACCT CACCGCCATC CTCAGTTCCC 
ACTGCCCATC TCATCCACCA GCCCGGCTCC AGGTCGTTGG AACCTTCTGG CATTTTACTT 
AAATCTGGCC ACTACGGCGG ACACTCAGAA GGTCTGGTGA GCAGATCTCC AGCTGTGGAG 
TCTCCTTCCG TAACTGTCAA CCACTACTCT CCCAACTCCC ATCAGCACAT AGACTGGAAA 
AACTATAAAA CTTACAAAGA GTATATTGAT AACCGGCGCT TGCACATGGG CTGTCGGACC 
ATTCAAGAAA GGTTAGATAG TTTAAGAGCC GCCTCTCAGA GCACAGCGGA TTATAACCAG 
GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA CGGCGTCGGA GCACCTCTCA GGACCGAGTG 
CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC GTGTCCCAGG AGAGGCTGGA GGACTCTGTG 
TTGATGATGT ATTGTCCGAG GAGTGCCTCT CAAGGTGCGC TGACATCCCC TTCTATTAGT 
TTTAGTAACC ACAGAACTCG CTCGTGGGAT TACATCGAGG GACAGGGTGA AACCTCGGAA 
AACGTCCATT CTGAATGTCC ACCGCCGGAT GCAAATGGTG AACGAAAGCA GACTTACAAG 
TGGAGTGGGT TCACCGAACA GGACGATCGT CGAGGTATTT ACGAGAGACC CAGGCAGCAA 
GAAATTCACA AATCTTTCCG AGGCTCCAAT TTTACAGTGG CTCCCAGTGT GGTGAATTCT 
GACAGCAGGA GAGTGAGCAG CAGAATCGGG GGATCAGTGT CTCAGTTTAA AAAAATTCCA 
GCAGATCTAA AACCCCTGCA GCCCAGCAGA AATTTTCAAA CTGCTTGTGG AATATCCCAG 
CCTCGAGGGA TTTCCCAAGA CAGGTCACCT CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA 
GGGCCTTCTA TGCATGTCGC GAAACCGCCC TCCAGCCAGA ACTCATTTCT TTCTATCAAA 
GACCAAAGAC CAGTAAATCA CTTGCATCAA AACAGTTTAT TGAATCAGCA GCCATGGTTA 
CGGACGGAAA GTGCCCCCGA TCACCAAGTG GACACTGGGA AGCCCCCTTC TCTTTCCGGA 
GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT GAGAATGCTA GCACTCCAGA TTTTGAGTTA 
TCCGTCTCTC AGAGGAATCA AGATTTAAAT GTACAAGAGG TGGAAATTCA GCAATCGAAT 
GCTGTAGATA ATAAAGAAAC TGTCATCCTA AGAGAAAAAC CTCCGTCTGG TCGCCAGACA 
CCGCAGCCTT TAAGGCATCA GTCTTACATC TTAGCAGTGA GTGACCAGGA GACAGGCTCA 
GACACCACCT GCTGGCTGCC TAATGATGCT CGCCGGGAGG TCCATATAAA AAGGATGGAG 
GAGAGGAAGG CCTCAAGTAC CACTCCACCT GGTGATTCCT TGGCTTCCAT CCCATTTATA 
GATGAACCTA CCAGCCCTAG CATCGATCAC GACATTTCTC ACATCCCCGC CTCTGCGGTC 
ATCTCTGCCT CCACCTCCCA GGTACCCTCC ATAGCAACGG TCCCTCCCAG CCTCACAACT 
TCAGCTCCAT TAATCCGACG TCAGCTCTCC CATGACCAGG AATCTGTTGG ACCTCCCAGC 
CTGGATGCCC AGCCCAGCTC AAAGACAGAG CGATCCAAAT CATATGATGA GGGCCTGGAT 
GATTACAGAG AAGATGCAAA ACTGTCTTTT AAACATGTAT CTAGCCTGAA GGGAATCAAG 
ATCTTAGACA GTCAAAAGTC ATCAGAAGAT TCCGGATCCA GGAAAGACTC TTCCTCAGAG 
GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG CTTCATTTCA GGCCACTCGT CACTGATAAG 
GGCAAGCGCG TTGGTGGAAG TATTCGGCCA TGGAAGCAGA TGTATGTTGT ACTTCGGGGC 
CATTCGCTGT ATCTGTACAA AGATAAACGA GAACAGACGA CTCCGTCTGA GGAGGAGCAG 
CCCATCAGTG TTAACGCCTG CTTAATAGAC ATCTCTTACA GCGAGACCAA GAGGAAGAAT 
GTCTTTCGAC TCACCACGTC TGACTGCGAG TGCCTGTTTC AGGCTGAAGA CAGGGATGAC 
ATGTTAGCCT GGATCAAAAC CATCCAGGAG AGCAGCAATT TAAACGAGGA GGACACTGGA 
GTCACTAACA GGGACCTAAT TAGTCGAAGA ATAAAAGAAT ACAACAGTCT AATGAGCAGC 
AAAGCAGAGC CATTGCCGAA GACCCCTCGC CAGAGTCTCA GCATCCGGCA GACCCTACTT 
GGTGCTAAAG CAGAGCCTCG GACTCAGAGT CCACACTCTC CCAAAGAGGA GTCAGAAAGG 
AAGCTTCTTA GTAAAGATGA TACCAGTCCT CCAAAAGACA AAGGCACGTG GAGAAAAGGC 
ATTCCAAGTA TTATGAGAAA GACATTTGAG AAAAAGCCTA CTGCTACGGG AACATTCGGG 
GTCAGACTGG ACGACTGCCC ACCAGCTCAT ACAAACCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGTA AATTAGTTGA AGAAAGAGGT CTGGAATATA CAGGTATTTA CAGAGTCCCT 
GGAAACAATG CAGCCATCTC CAGTATGCAG GAGGAGCTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATCA GCAGTTTATT AAAATCCTTC 
TTCAGAAAAC TCCCAGAACC TCTCTTCACG AATGATAAAT ACGCCGACTT TATTGAAGCC 
AATCGTAAGG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCATGATTTG 
CCCGAACATC ATTATGAAAC ACTCAAGTTT CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCCA TAGTGTTTGG TCCAACTCTT 
GTGAGAACCT CCGAAGATAA CATGACACAC ATGGTCACTC ACATGCCAGA TCAGTACAAG 
ATCGTAGAGA CACTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA 
CCTCTTACAA CAGTGCAGGA GGAAAACACA GTAGACTCCC AGCCAGTGCC AAACATAGAT 
CATTTACTCA CCAACATTGG AAGGACAGGC GTCTCCCCTG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CAACAAAATC TAAGGGTTCT TGGGGATCCG GAAAAGACCA GTACAGCAAG 
GACCTGCTTG TATCCTCCAT CTTCGCGGCT GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA 
AAAGCACAAC CCAGCAGCTC GGAAGACGAG CTGGACAATG TGTTTTTCAA GAAAGAAAAC 
TCAGAGCAGG GTCACCACGA CATTAAAGAA GAGTCCAAAA AAGAGAGTGA GACACCAGGC 
CGGAAACAAA GGACCGTTGC TCCCAAAGAA AACAACACGA AGAAAGACAG CAGCGGAGGT 
GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC 
ACCAAGCAGA GCCGGTCACC CACCCTGAGC TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC 
AGGGCCCTCG CAGCCCAGAA GACCTCCCAC CTGGAAGACA CGGGGTCCGA CTCCAGCACC 
CCACTCAGCA CTCACGCCCC GGCTCCCCCG GCAAGCTTTC CCACCAAGAA ACCTCCCAGC 
CCCGAGCCCA AGCACAGCGA GCTCCTGGTC AGTGTCGGCA GCATCACCTC CGACCACGCC 
GGCACACCGT CTGCTCCCTA CCTGGCCGGC CTCGACTCCA GCCGGCTGAG CCCCGAGGTG 
CAGTCCATGG CCGAGAGCCG GGGATTTGAA GCTGATGACG AGAGGAGCGA GCTGGTCAGC 
GAGGGCCGGC CAGTGGAGAC AGACAGCGAG AGCGACTTTC CGGTCTTCCC CGCCGCCCCG 
GCCTCGGACA GGCTCTTCCG AGGAAAACTC CAAGAGGCCG CGAGGACCAG CCGGAGAAAC 
TCAGAAGGCA GTGAGGTCAG CTGCACGGAA GGAAGTTTAA CACCAAGTTT AGAAAGCCGG 
AGACAGCTCT TCAGCTCCCA TAAACTGATC GAGTGTGACA CTCTGTCCAG GAAGAAATCC 
GCCAGGTTCA AGTCAGACAG TGGGAGTCTA GGAGATGCCA AGAATGAGAA AGAAGCCCCT 
TCCATCACCA AAGTGTTCGA CGTTATGAAA AAAGGAAAAT CCACCGGAAA TTTACTGACA 
CCACCAGCCA GAAGCGAATC GGACAAACAG GAACCCACTT GGAAAACAAA AATAGCCGAT 
CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC GATGACATGT TTGGAGTAGG AAGTCAAAAA 
ACCATCGCCG AACCTGCCAA AAGGAAAAAC ATCAAACGCA GACACACGCT GGGTGGGCAC 
AGGGATGCTA CTGAAATGAG TGTTCTGAAT TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC 
AGAGAATCCG AACTTTCAGC TGTGAATCGA TTGAAGCCCA AATGCTCCGC CCAGGACCTG 
TCCATCTCAG ACTGGCTGGC CAGGGAGCGG CTACGCACCA GTATGACTGA CCTGAGCAGA 
GGGGACACGG GGGACCCCCG GCCTGAGAGT CTTGGCACCT TAGAAATACC AACCAGGGAC 
CCGCCCCTGT CTTTCCAGTC TGATGCAGAC AGTTCTTCCA GCACCTTGGC TTCAACTAAC 
AGGGCCCCTC TTTCCACACC ATCACAGTCA CCTGACCAAA TAAATGGAGA AAGCTTCCAG 
AACACGAGCC AAAACTCCAG TTCTGCAGCC AGGGTCCAAC CTCATCAACT GTCTGAAACC 
CCAGATCACA AAGCACAGTT CCATCCCTGT CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809500.1
CDS	202..5577
Translation	MPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_010811198.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811198.2

ORF Insert Sequence:

ATGCCAAAAG ATGAAGACAT TCTCCAAGTG GCGTATTCTC AAGATGCCTA CCTGAAAGGC 
AATGAAGCCT ACAGCGGCAA TGCCCGCAAC ATCCCTGAGC CCCCACCAGT CTGCTACCCC 
TGGCCGTCAT CCGCCCCTCC CGCCATGGCG CAGCCCCCTG AACCACCTCC TCCAGACTCC 
ACAGCAAGCA AACCGCAGAC TAGCCCCCCA GTATTGACGC AGCCCGGCAG GGCATACAGG 
ATGGAAATCC AAGTGCCTCC GTCACCACCA GAGGTGGCCA AGTCCAACAC AGCAGTGTGT 
GTCTGCAATG AGAGTGTGAG GACCGTCATT GTACCTTCTG AGAAGGTTGT AGATTTATTA 
CCTAACAGAA GCAACCACAC AGTTCCCGCT CACAGAACTG AGGAAGTGAG GTATGGCCGA 
AACGAACAGC CCTCTTTCAA AACAGCGACG AGAACCACCT CACCGCCATC CTCAGTTCCC 
ACTGCCCATC TCATCCACCA GCCCGGCTCC AGGTCGTTGG AACCTTCTGG CATTTTACTT 
AAATCTGGCC ACTACGGCGG ACACTCAGAA GGTCTGGTGA GCAGATCTCC AGCTGTGGAG 
TCTCCTTCCG TAACTGTCAA CCACTACTCT CCCAACTCCC ATCAGCACAT AGACTGGAAA 
AACTATAAAA CTTACAAAGA GTATATTGAT AACCGGCGCT TGCACATGGG CTGTCGGACC 
ATTCAAGAAA GGTTAGATAG TTTAAGAGCC GCCTCTCAGA GCACAGCGGA TTATAACCAG 
GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA CGGCGTCGGA GCACCTCTCA GGACCGAGTG 
CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC GTGTCCCAGG AGAGGCTGGA GGACTCTGTG 
TTGATGATGT ATTGTCCGAG GAGTGCCTCT CAAGGTGCGC TGACATCCCC TTCTATTAGT 
TTTAGTAACC ACAGAACTCG CTCGTGGGAT TACATCGAGG GACAGGGTGA AACCTCGGAA 
AACGTCCATT CTGAATGTCC ACCGCCGGAT GCAAATGGTG AACGAAAGCA GACTTACAAG 
TGGAGTGGGT TCACCGAACA GGACGATCGT CGAGGTATTT ACGAGAGACC CAGGCAGCAA 
GAAATTCACA AATCTTTCCG AGGCTCCAAT TTTACAGTGG CTCCCAGTGT GGTGAATTCT 
GACAGCAGGA GAGTGAGCAG CAGAATCGGG GGATCAGTGT CTCAGTTTAA AAAAATTCCA 
GCAGATCTAA AACCCCTGCA GCCCAGCAGA AATTTTCAAA CTGCTTGTGG AATATCCCAG 
CCTCGAGGGA TTTCCCAAGA CAGGTCACCT CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA 
GGGCCTTCTA TGCATGTCGC GAAACCGCCC TCCAGCCAGA ACTCATTTCT TTCTATCAAA 
GACCAAAGAC CAGTAAATCA CTTGCATCAA AACAGTTTAT TGAATCAGCA GCCATGGTTA 
CGGACGGAAA GTGCCCCCGA TCACCAAGTG GACACTGGGA AGCCCCCTTC TCTTTCCGGA 
GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT GAGAATGCTA GCACTCCAGA TTTTGAGTTA 
TCCGTCTCTC AGAGGAATCA AGATTTAAAT GTACAAGAGG TGGAAATTCA GCAATCGAAT 
GCTGTAGATA ATAAAGAAAC TGTCATCCTA AGAGAAAAAC CTCCGTCTGG TCGCCAGACA 
CCGCAGCCTT TAAGGCATCA GTCTTACATC TTAGCAGTGA GTGACCAGGA GACAGGCTCA 
GACACCACCT GCTGGCTGCC TAATGATGCT CGCCGGGAGG TCCATATAAA AAGGATGGAG 
GAGAGGAAGG CCTCAAGTAC CACTCCACCT GGTGATTCCT TGGCTTCCAT CCCATTTATA 
GATGAACCTA CCAGCCCTAG CATCGATCAC GACATTTCTC ACATCCCCGC CTCTGCGGTC 
ATCTCTGCCT CCACCTCCCA GGTACCCTCC ATAGCAACGG TCCCTCCCAG CCTCACAACT 
TCAGCTCCAT TAATCCGACG TCAGCTCTCC CATGACCAGG AATCTGTTGG ACCTCCCAGC 
CTGGATGCCC AGCCCAGCTC AAAGACAGAG CGATCCAAAT CATATGATGA GGGCCTGGAT 
GATTACAGAG AAGATGCAAA ACTGTCTTTT AAACATGTAT CTAGCCTGAA GGGAATCAAG 
ATCTTAGACA GTCAAAAGTC ATCAGAAGAT TCCGGATCCA GGAAAGACTC TTCCTCAGAG 
GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG CTTCATTTCA GGCCACTCGT CACTGATAAG 
GGCAAGCGCG TTGGTGGAAG TATTCGGCCA TGGAAGCAGA TGTATGTTGT ACTTCGGGGC 
CATTCGCTGT ATCTGTACAA AGATAAACGA GAACAGACGA CTCCGTCTGA GGAGGAGCAG 
CCCATCAGTG TTAACGCCTG CTTAATAGAC ATCTCTTACA GCGAGACCAA GAGGAAGAAT 
GTCTTTCGAC TCACCACGTC TGACTGCGAG TGCCTGTTTC AGGCTGAAGA CAGGGATGAC 
ATGTTAGCCT GGATCAAAAC CATCCAGGAG AGCAGCAATT TAAACGAGGA GGACACTGGA 
GTCACTAACA GGGACCTAAT TAGTCGAAGA ATAAAAGAAT ACAACAGTCT AATGAGCAGC 
AAAGCAGAGC CATTGCCGAA GACCCCTCGC CAGAGTCTCA GCATCCGGCA GACCCTACTT 
GGTGCTAAAG CAGAGCCTCG GACTCAGAGT CCACACTCTC CCAAAGAGGA GTCAGAAAGG 
AAGCTTCTTA GTAAAGATGA TACCAGTCCT CCAAAAGACA AAGGCACGTG GAGAAAAGGC 
ATTCCAAGTA TTATGAGAAA GACATTTGAG AAAAAGCCTA CTGCTACGGG AACATTCGGG 
GTCAGACTGG ACGACTGCCC ACCAGCTCAT ACAAACCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGTA AATTAGTTGA AGAAAGAGGT CTGGAATATA CAGGTATTTA CAGAGTCCCT 
GGAAACAATG CAGCCATCTC CAGTATGCAG GAGGAGCTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATCA GCAGTTTATT AAAATCCTTC 
TTCAGAAAAC TCCCAGAACC TCTCTTCACG AATGATAAAT ACGCCGACTT TATTGAAGCC 
AATCGTAAGG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCATGATTTG 
CCCGAACATC ATTATGAAAC ACTCAAGTTT CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCCA TAGTGTTTGG TCCAACTCTT 
GTGAGAACCT CCGAAGATAA CATGACACAC ATGGTCACTC ACATGCCAGA TCAGTACAAG 
ATCGTAGAGA CACTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA 
CCTCTTACAA CAGTGCAGGA GGAAAACACA GTAGACTCCC AGCCAGTGCC AAACATAGAT 
CATTTACTCA CCAACATTGG AAGGACAGGC GTCTCCCCTG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CAACAAAATC TAAGGGTTCT TGGGGATCCG GAAAAGACCA GTACAGCAAG 
GACCTGCTTG TATCCTCCAT CTTCGCGGCT GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA 
AAAGCACAAC CCAGCAGCTC GGAAGACGAG CTGGACAATG TGTTTTTCAA GAAAGAAAAC 
TCAGAGCAGG GTCACCACGA CATTAAAGAA GAGTCCAAAA AAGAGAGTGA GACACCAGGC 
CGGAAACAAA GGACCGTTGC TCCCAAAGAA AACAACACGA AGAAAGACAG CAGCGGAGGT 
GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC 
ACCAAGCAGA GCCGGTCACC CACCCTGAGC TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC 
AGGGCCCTCG CAGCCCAGAA GACCTCCCAC CTGGAAGACA CGGGGTCCGA CTCCAGCACC 
CCACTCAGCA CTCACGCCCC GGCTCCCCCG GCAAGCTTTC CCACCAAGAA ACCTCCCAGC 
CCCGAGCCCA AGCACAGCGA GCTCCTGGTC AGTGTCGGCA GCATCACCTC CGACCACGCC 
GGCACACCGT CTGCTCCCTA CCTGGCCGGC CTCGACTCCA GCCGGCTGAG CCCCGAGGTG 
CAGTCCATGG CCGAGAGCCG GGGATTTGAA GCTGATGACG AGAGGAGCGA GCTGGTCAGC 
GAGGGCCGGC CAGTGGAGAC AGACAGCGAG AGCGACTTTC CGGTCTTCCC CGCCGCCCCG 
GCCTCGGACA GGCTCTTCCG AGGAAAACTC CAAGAGGCCG CGAGGACCAG CCGGAGAAAC 
TCAGAAGGCA GTGAGGTCAG CTGCACGGAA GGAAGTTTAA CACCAAGTTT AGAAAGCCGG 
AGACAGCTCT TCAGCTCCCA TAAACTGATC GAGTGTGACA CTCTGTCCAG GAAGAAATCC 
GCCAGGTTCA AGTCAGACAG TGGGAGTCTA GGAGATGCCA AGAATGAGAA AGAAGCCCCT 
TCCATCACCA AAGTGTTCGA CGTTATGAAA AAAGGAAAAT CCACCGGAAA TTTACTGACA 
CCACCAGCCA GAAGCGAATC GGACAAACAG GAACCCACTT GGAAAACAAA AATAGCCGAT 
CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC GATGACATGT TTGGAGTAGG AAGTCAAAAA 
ACCATCGCCG AACCTGCCAA AAGGAAAAAC ATCAAACGCA GACACACGCT GGGTGGGCAC 
AGGGATGCTA CTGAAATGAG TGTTCTGAAT TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC 
AGAGAATCCG AACTTTCAGC TGTGAATCGA TTGAAGCCCA AATGCTCCGC CCAGGACCTG 
TCCATCTCAG ACTGGCTGGC CAGGGAGCGG CTACGCACCA GTATGACTGA CCTGAGCAGA 
GGGGACACGG GGGACCCCCG GCCTGAGAGT CTTGGCACCT TAGAAATACC AACCAGGGAC 
CCGCCCCTGT CTTTCCAGTC TGATGCAGAC AGTTCTTCCA GCACCTTGGC TTCAACTAAC 
AGGGCCCCTC TTTCCACACC ATCACAGTCA CCTGACCAAA TAAATGGAGA AAGCTTCCAG 
AACACGAGCC AAAACTCCAG TTCTGCAGCC AGGGTCCAAC CTCATCAACT GTCTGAAACC 
CCAGATCACA AAGCACAGTT CCATCCCTGT CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_010822425.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820727.1 Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X8, mRNA.

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CloneID	OBa53932
Clone ID Related Accession (Same CDS sequence)	XM_010811198.2 , XM_010822425.2
Accession Version	XM_010822425.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho GTPase-activating protein 21 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646129.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAAAG ATGAAGACAT TCTCCAAGTG GCGTATTCTC AAGATGCCTA CCTGAAAGGC 
AATGAAGCCT ACAGCGGCAA TGCCCGCAAC ATCCCTGAGC CCCCACCAGT CTGCTACCCC 
TGGCCGTCAT CCGCCCCTCC CGCCATGGCG CAGCCCCCTG AACCACCTCC TCCAGACTCC 
ACAGCAAGCA AACCGCAGAC TAGCCCCCCA GTATTGACGC AGCCCGGCAG GGCATACAGG 
ATGGAAATCC AAGTGCCTCC GTCACCACCA GAGGTGGCCA AGTCCAACAC AGCAGTGTGT 
GTCTGCAATG AGAGTGTGAG GACCGTCATT GTACCTTCTG AGAAGGTTGT AGATTTATTA 
CCTAACAGAA GCAACCACAC AGTTCCCGCT CACAGAACTG AGGAAGTGAG GTATGGCCGA 
AACGAACAGC CCTCTTTCAA AACAGCGACG AGAACCACCT CACCGCCATC CTCAGTTCCC 
ACTGCCCATC TCATCCACCA GCCCGGCTCC AGGTCGTTGG AACCTTCTGG CATTTTACTT 
AAATCTGGCC ACTACGGCGG ACACTCAGAA GGTCTGGTGA GCAGATCTCC AGCTGTGGAG 
TCTCCTTCCG TAACTGTCAA CCACTACTCT CCCAACTCCC ATCAGCACAT AGACTGGAAA 
AACTATAAAA CTTACAAAGA GTATATTGAT AACCGGCGCT TGCACATGGG CTGTCGGACC 
ATTCAAGAAA GGTTAGATAG TTTAAGAGCC GCCTCTCAGA GCACAGCGGA TTATAACCAG 
GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA CGGCGTCGGA GCACCTCTCA GGACCGAGTG 
CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC GTGTCCCAGG AGAGGCTGGA GGACTCTGTG 
TTGATGATGT ATTGTCCGAG GAGTGCCTCT CAAGGTGCGC TGACATCCCC TTCTATTAGT 
TTTAGTAACC ACAGAACTCG CTCGTGGGAT TACATCGAGG GACAGGGTGA AACCTCGGAA 
AACGTCCATT CTGAATGTCC ACCGCCGGAT GCAAATGGTG AACGAAAGCA GACTTACAAG 
TGGAGTGGGT TCACCGAACA GGACGATCGT CGAGGTATTT ACGAGAGACC CAGGCAGCAA 
GAAATTCACA AATCTTTCCG AGGCTCCAAT TTTACAGTGG CTCCCAGTGT GGTGAATTCT 
GACAGCAGGA GAGTGAGCAG CAGAATCGGG GGATCAGTGT CTCAGTTTAA AAAAATTCCA 
GCAGATCTAA AACCCCTGCA GCCCAGCAGA AATTTTCAAA CTGCTTGTGG AATATCCCAG 
CCTCGAGGGA TTTCCCAAGA CAGGTCACCT CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA 
GGGCCTTCTA TGCATGTCGC GAAACCGCCC TCCAGCCAGA ACTCATTTCT TTCTATCAAA 
GACCAAAGAC CAGTAAATCA CTTGCATCAA AACAGTTTAT TGAATCAGCA GCCATGGTTA 
CGGACGGAAA GTGCCCCCGA TCACCAAGTG GACACTGGGA AGCCCCCTTC TCTTTCCGGA 
GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT GAGAATGCTA GCACTCCAGA TTTTGAGTTA 
TCCGTCTCTC AGAGGAATCA AGATTTAAAT GTACAAGAGG TGGAAATTCA GCAATCGAAT 
GCTGTAGATA ATAAAGAAAC TGTCATCCTA AGAGAAAAAC CTCCGTCTGG TCGCCAGACA 
CCGCAGCCTT TAAGGCATCA GTCTTACATC TTAGCAGTGA GTGACCAGGA GACAGGCTCA 
GACACCACCT GCTGGCTGCC TAATGATGCT CGCCGGGAGG TCCATATAAA AAGGATGGAG 
GAGAGGAAGG CCTCAAGTAC CACTCCACCT GGTGATTCCT TGGCTTCCAT CCCATTTATA 
GATGAACCTA CCAGCCCTAG CATCGATCAC GACATTTCTC ACATCCCCGC CTCTGCGGTC 
ATCTCTGCCT CCACCTCCCA GGTACCCTCC ATAGCAACGG TCCCTCCCAG CCTCACAACT 
TCAGCTCCAT TAATCCGACG TCAGCTCTCC CATGACCAGG AATCTGTTGG ACCTCCCAGC 
CTGGATGCCC AGCCCAGCTC AAAGACAGAG CGATCCAAAT CATATGATGA GGGCCTGGAT 
GATTACAGAG AAGATGCAAA ACTGTCTTTT AAACATGTAT CTAGCCTGAA GGGAATCAAG 
ATCTTAGACA GTCAAAAGTC ATCAGAAGAT TCCGGATCCA GGAAAGACTC TTCCTCAGAG 
GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG CTTCATTTCA GGCCACTCGT CACTGATAAG 
GGCAAGCGCG TTGGTGGAAG TATTCGGCCA TGGAAGCAGA TGTATGTTGT ACTTCGGGGC 
CATTCGCTGT ATCTGTACAA AGATAAACGA GAACAGACGA CTCCGTCTGA GGAGGAGCAG 
CCCATCAGTG TTAACGCCTG CTTAATAGAC ATCTCTTACA GCGAGACCAA GAGGAAGAAT 
GTCTTTCGAC TCACCACGTC TGACTGCGAG TGCCTGTTTC AGGCTGAAGA CAGGGATGAC 
ATGTTAGCCT GGATCAAAAC CATCCAGGAG AGCAGCAATT TAAACGAGGA GGACACTGGA 
GTCACTAACA GGGACCTAAT TAGTCGAAGA ATAAAAGAAT ACAACAGTCT AATGAGCAGC 
AAAGCAGAGC CATTGCCGAA GACCCCTCGC CAGAGTCTCA GCATCCGGCA GACCCTACTT 
GGTGCTAAAG CAGAGCCTCG GACTCAGAGT CCACACTCTC CCAAAGAGGA GTCAGAAAGG 
AAGCTTCTTA GTAAAGATGA TACCAGTCCT CCAAAAGACA AAGGCACGTG GAGAAAAGGC 
ATTCCAAGTA TTATGAGAAA GACATTTGAG AAAAAGCCTA CTGCTACGGG AACATTCGGG 
GTCAGACTGG ACGACTGCCC ACCAGCTCAT ACAAACCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGTA AATTAGTTGA AGAAAGAGGT CTGGAATATA CAGGTATTTA CAGAGTCCCT 
GGAAACAATG CAGCCATCTC CAGTATGCAG GAGGAGCTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATCA GCAGTTTATT AAAATCCTTC 
TTCAGAAAAC TCCCAGAACC TCTCTTCACG AATGATAAAT ACGCCGACTT TATTGAAGCC 
AATCGTAAGG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCATGATTTG 
CCCGAACATC ATTATGAAAC ACTCAAGTTT CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCCA TAGTGTTTGG TCCAACTCTT 
GTGAGAACCT CCGAAGATAA CATGACACAC ATGGTCACTC ACATGCCAGA TCAGTACAAG 
ATCGTAGAGA CACTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA 
CCTCTTACAA CAGTGCAGGA GGAAAACACA GTAGACTCCC AGCCAGTGCC AAACATAGAT 
CATTTACTCA CCAACATTGG AAGGACAGGC GTCTCCCCTG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CAACAAAATC TAAGGGTTCT TGGGGATCCG GAAAAGACCA GTACAGCAAG 
GACCTGCTTG TATCCTCCAT CTTCGCGGCT GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA 
AAAGCACAAC CCAGCAGCTC GGAAGACGAG CTGGACAATG TGTTTTTCAA GAAAGAAAAC 
TCAGAGCAGG GTCACCACGA CATTAAAGAA GAGTCCAAAA AAGAGAGTGA GACACCAGGC 
CGGAAACAAA GGACCGTTGC TCCCAAAGAA AACAACACGA AGAAAGACAG CAGCGGAGGT 
GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC 
ACCAAGCAGA GCCGGTCACC CACCCTGAGC TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC 
AGGGCCCTCG CAGCCCAGAA GACCTCCCAC CTGGAAGACA CGGGGTCCGA CTCCAGCACC 
CCACTCAGCA CTCACGCCCC GGCTCCCCCG GCAAGCTTTC CCACCAAGAA ACCTCCCAGC 
CCCGAGCCCA AGCACAGCGA GCTCCTGGTC AGTGTCGGCA GCATCACCTC CGACCACGCC 
GGCACACCGT CTGCTCCCTA CCTGGCCGGC CTCGACTCCA GCCGGCTGAG CCCCGAGGTG 
CAGTCCATGG CCGAGAGCCG GGGATTTGAA GCTGATGACG AGAGGAGCGA GCTGGTCAGC 
GAGGGCCGGC CAGTGGAGAC AGACAGCGAG AGCGACTTTC CGGTCTTCCC CGCCGCCCCG 
GCCTCGGACA GGCTCTTCCG AGGAAAACTC CAAGAGGCCG CGAGGACCAG CCGGAGAAAC 
TCAGAAGGCA GTGAGGTCAG CTGCACGGAA GGAAGTTTAA CACCAAGTTT AGAAAGCCGG 
AGACAGCTCT TCAGCTCCCA TAAACTGATC GAGTGTGACA CTCTGTCCAG GAAGAAATCC 
GCCAGGTTCA AGTCAGACAG TGGGAGTCTA GGAGATGCCA AGAATGAGAA AGAAGCCCCT 
TCCATCACCA AAGTGTTCGA CGTTATGAAA AAAGGAAAAT CCACCGGAAA TTTACTGACA 
CCACCAGCCA GAAGCGAATC GGACAAACAG GAACCCACTT GGAAAACAAA AATAGCCGAT 
CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC GATGACATGT TTGGAGTAGG AAGTCAAAAA 
ACCATCGCCG AACCTGCCAA AAGGAAAAAC ATCAAACGCA GACACACGCT GGGTGGGCAC 
AGGGATGCTA CTGAAATGAG TGTTCTGAAT TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC 
AGAGAATCCG AACTTTCAGC TGTGAATCGA TTGAAGCCCA AATGCTCCGC CCAGGACCTG 
TCCATCTCAG ACTGGCTGGC CAGGGAGCGG CTACGCACCA GTATGACTGA CCTGAGCAGA 
GGGGACACGG GGGACCCCCG GCCTGAGAGT CTTGGCACCT TAGAAATACC AACCAGGGAC 
CCGCCCCTGT CTTTCCAGTC TGATGCAGAC AGTTCTTCCA GCACCTTGGC TTCAACTAAC 
AGGGCCCCTC TTTCCACACC ATCACAGTCA CCTGACCAAA TAAATGGAGA AAGCTTCCAG 
AACACGAGCC AAAACTCCAG TTCTGCAGCC AGGGTCCAAC CTCATCAACT GTCTGAAACC 
CCAGATCACA AAGCACAGTT CCATCCCTGT CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820727.1
CDS	202..5577
Translation	MPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWPSSAPPAMAQPPEPPPPDSTASKPQTSPPVLTQPGRAYRMEIQVPPSPPEVAKSNTAVCVCNESVRTVIVPSEKVVDLLPNRSNHTVPAHRTEEVRYGRNEQPSFKTATRTTSPPSSVPTAHLIHQPGSRSLEPSGILLKSGHYGGHSEGLVSRSPAVESPSVTVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHMGCRTIQERLDSLRAASQSTADYNQVVLNRAALQVRRRSTSQDRVPQSIQVRQRSVSQERLEDSVLMMYCPRSASQGALTSPSISFSNHRTRSWDYIEGQGETSENVHSECPPPDANGERKQTYKWSGFTEQDDRRGIYERPRQQEIHKSFRGSNFTVAPSVVNSDSRRVSSRIGGSVSQFKKIPADLKPLQPSRNFQTACGISQPRGISQDRSPLGKVRSNSLKGPSMHVAKPPSSQNSFLSIKDQRPVNHLHQNSLLNQQPWLRTESAPDHQVDTGKPPSLSGASVKPPALASENASTPDFELSVSQRNQDLNVQEVEIQQSNAVDNKETVILREKPPSGRQTPQPLRHQSYILAVSDQETGSDTTCWLPNDARREVHIKRMEERKASSTTPPGDSLASIPFIDEPTSPSIDHDISHIPASAVISASTSQVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDAQPSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKILDSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLMSSKAEPLPKTPRQSLSIRQTLLGAKAEPRTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEENTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSKDLLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENSEQGHHDIKEESKKESETPGRKQRTVAPKENNTKKDSSGGGRAEQRAPRGESLEPPAPQSTKQSRSPTLSCRLAVLRESPRALAAQKTSHLEDTGSDSSTPLSTHAPAPPASFPTKKPPSPEPKHSELLVSVGSITSDHAGTPSAPYLAGLDSSRLSPEVQSMAESRGFEADDERSELVSEGRPVETDSESDFPVFPAAPASDRLFRGKLQEAARTSRRNSEGSEVSCTEGSLTPSLESRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSITKVFDVMKKGKSTGNLLTPPARSESDKQEPTWKTKIADRLKLRPKAPADDMFGVGSQKTIAEPAKRKNIKRRHTLGGHRDATEMSVLNFWKAQEHSGDRESELSAVNRLKPKCSAQDLSISDWLARERLRTSMTDLSRGDTGDPRPESLGTLEIPTRDPPLSFQSDADSSSSTLASTNRAPLSTPSQSPDQINGESFQNTSQNSSSAARVQPHQLSETPDHKAQFHPCL

Target ORF information:

RefSeq Version	XM_010822425.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho GTPase activating protein 21 (ARHGAP21), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822425.2

ORF Insert Sequence:

ATGCCAAAAG ATGAAGACAT TCTCCAAGTG GCGTATTCTC AAGATGCCTA CCTGAAAGGC 
AATGAAGCCT ACAGCGGCAA TGCCCGCAAC ATCCCTGAGC CCCCACCAGT CTGCTACCCC 
TGGCCGTCAT CCGCCCCTCC CGCCATGGCG CAGCCCCCTG AACCACCTCC TCCAGACTCC 
ACAGCAAGCA AACCGCAGAC TAGCCCCCCA GTATTGACGC AGCCCGGCAG GGCATACAGG 
ATGGAAATCC AAGTGCCTCC GTCACCACCA GAGGTGGCCA AGTCCAACAC AGCAGTGTGT 
GTCTGCAATG AGAGTGTGAG GACCGTCATT GTACCTTCTG AGAAGGTTGT AGATTTATTA 
CCTAACAGAA GCAACCACAC AGTTCCCGCT CACAGAACTG AGGAAGTGAG GTATGGCCGA 
AACGAACAGC CCTCTTTCAA AACAGCGACG AGAACCACCT CACCGCCATC CTCAGTTCCC 
ACTGCCCATC TCATCCACCA GCCCGGCTCC AGGTCGTTGG AACCTTCTGG CATTTTACTT 
AAATCTGGCC ACTACGGCGG ACACTCAGAA GGTCTGGTGA GCAGATCTCC AGCTGTGGAG 
TCTCCTTCCG TAACTGTCAA CCACTACTCT CCCAACTCCC ATCAGCACAT AGACTGGAAA 
AACTATAAAA CTTACAAAGA GTATATTGAT AACCGGCGCT TGCACATGGG CTGTCGGACC 
ATTCAAGAAA GGTTAGATAG TTTAAGAGCC GCCTCTCAGA GCACAGCGGA TTATAACCAG 
GTGGTGCTGA ACCGCGCTGC CCTGCAGGTA CGGCGTCGGA GCACCTCTCA GGACCGAGTG 
CCCCAGTCCA TCCAGGTCCG GCAGCGCAGC GTGTCCCAGG AGAGGCTGGA GGACTCTGTG 
TTGATGATGT ATTGTCCGAG GAGTGCCTCT CAAGGTGCGC TGACATCCCC TTCTATTAGT 
TTTAGTAACC ACAGAACTCG CTCGTGGGAT TACATCGAGG GACAGGGTGA AACCTCGGAA 
AACGTCCATT CTGAATGTCC ACCGCCGGAT GCAAATGGTG AACGAAAGCA GACTTACAAG 
TGGAGTGGGT TCACCGAACA GGACGATCGT CGAGGTATTT ACGAGAGACC CAGGCAGCAA 
GAAATTCACA AATCTTTCCG AGGCTCCAAT TTTACAGTGG CTCCCAGTGT GGTGAATTCT 
GACAGCAGGA GAGTGAGCAG CAGAATCGGG GGATCAGTGT CTCAGTTTAA AAAAATTCCA 
GCAGATCTAA AACCCCTGCA GCCCAGCAGA AATTTTCAAA CTGCTTGTGG AATATCCCAG 
CCTCGAGGGA TTTCCCAAGA CAGGTCACCT CTTGGGAAAG TCCGAAGTAA CTCTCTGAAA 
GGGCCTTCTA TGCATGTCGC GAAACCGCCC TCCAGCCAGA ACTCATTTCT TTCTATCAAA 
GACCAAAGAC CAGTAAATCA CTTGCATCAA AACAGTTTAT TGAATCAGCA GCCATGGTTA 
CGGACGGAAA GTGCCCCCGA TCACCAAGTG GACACTGGGA AGCCCCCTTC TCTTTCCGGA 
GCTTCTGTTA AGCCCCCGGC TCTGGCAAGT GAGAATGCTA GCACTCCAGA TTTTGAGTTA 
TCCGTCTCTC AGAGGAATCA AGATTTAAAT GTACAAGAGG TGGAAATTCA GCAATCGAAT 
GCTGTAGATA ATAAAGAAAC TGTCATCCTA AGAGAAAAAC CTCCGTCTGG TCGCCAGACA 
CCGCAGCCTT TAAGGCATCA GTCTTACATC TTAGCAGTGA GTGACCAGGA GACAGGCTCA 
GACACCACCT GCTGGCTGCC TAATGATGCT CGCCGGGAGG TCCATATAAA AAGGATGGAG 
GAGAGGAAGG CCTCAAGTAC CACTCCACCT GGTGATTCCT TGGCTTCCAT CCCATTTATA 
GATGAACCTA CCAGCCCTAG CATCGATCAC GACATTTCTC ACATCCCCGC CTCTGCGGTC 
ATCTCTGCCT CCACCTCCCA GGTACCCTCC ATAGCAACGG TCCCTCCCAG CCTCACAACT 
TCAGCTCCAT TAATCCGACG TCAGCTCTCC CATGACCAGG AATCTGTTGG ACCTCCCAGC 
CTGGATGCCC AGCCCAGCTC AAAGACAGAG CGATCCAAAT CATATGATGA GGGCCTGGAT 
GATTACAGAG AAGATGCAAA ACTGTCTTTT AAACATGTAT CTAGCCTGAA GGGAATCAAG 
ATCTTAGACA GTCAAAAGTC ATCAGAAGAT TCCGGATCCA GGAAAGACTC TTCCTCAGAG 
GTTTTCAGCG ATGCTGCCAA GGAAGGGTGG CTTCATTTCA GGCCACTCGT CACTGATAAG 
GGCAAGCGCG TTGGTGGAAG TATTCGGCCA TGGAAGCAGA TGTATGTTGT ACTTCGGGGC 
CATTCGCTGT ATCTGTACAA AGATAAACGA GAACAGACGA CTCCGTCTGA GGAGGAGCAG 
CCCATCAGTG TTAACGCCTG CTTAATAGAC ATCTCTTACA GCGAGACCAA GAGGAAGAAT 
GTCTTTCGAC TCACCACGTC TGACTGCGAG TGCCTGTTTC AGGCTGAAGA CAGGGATGAC 
ATGTTAGCCT GGATCAAAAC CATCCAGGAG AGCAGCAATT TAAACGAGGA GGACACTGGA 
GTCACTAACA GGGACCTAAT TAGTCGAAGA ATAAAAGAAT ACAACAGTCT AATGAGCAGC 
AAAGCAGAGC CATTGCCGAA GACCCCTCGC CAGAGTCTCA GCATCCGGCA GACCCTACTT 
GGTGCTAAAG CAGAGCCTCG GACTCAGAGT CCACACTCTC CCAAAGAGGA GTCAGAAAGG 
AAGCTTCTTA GTAAAGATGA TACCAGTCCT CCAAAAGACA AAGGCACGTG GAGAAAAGGC 
ATTCCAAGTA TTATGAGAAA GACATTTGAG AAAAAGCCTA CTGCTACGGG AACATTCGGG 
GTCAGACTGG ACGACTGCCC ACCAGCTCAT ACAAACCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGTA AATTAGTTGA AGAAAGAGGT CTGGAATATA CAGGTATTTA CAGAGTCCCT 
GGAAACAATG CAGCCATCTC CAGTATGCAG GAGGAGCTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATCA GCAGTTTATT AAAATCCTTC 
TTCAGAAAAC TCCCAGAACC TCTCTTCACG AATGATAAAT ACGCCGACTT TATTGAAGCC 
AATCGTAAGG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCATGATTTG 
CCCGAACATC ATTATGAAAC ACTCAAGTTT CTTTCGGCTC ATCTGAAGAC AGTAGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCCA TAGTGTTTGG TCCAACTCTT 
GTGAGAACCT CCGAAGATAA CATGACACAC ATGGTCACTC ACATGCCAGA TCAGTACAAG 
ATCGTAGAGA CACTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAA 
CCTCTTACAA CAGTGCAGGA GGAAAACACA GTAGACTCCC AGCCAGTGCC AAACATAGAT 
CATTTACTCA CCAACATTGG AAGGACAGGC GTCTCCCCTG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CAACAAAATC TAAGGGTTCT TGGGGATCCG GAAAAGACCA GTACAGCAAG 
GACCTGCTTG TATCCTCCAT CTTCGCGGCT GCCAGTCGCA AGAGGAAAAA GCCAAAAGAA 
AAAGCACAAC CCAGCAGCTC GGAAGACGAG CTGGACAATG TGTTTTTCAA GAAAGAAAAC 
TCAGAGCAGG GTCACCACGA CATTAAAGAA GAGTCCAAAA AAGAGAGTGA GACACCAGGC 
CGGAAACAAA GGACCGTTGC TCCCAAAGAA AACAACACGA AGAAAGACAG CAGCGGAGGT 
GGCAGAGCTG AGCAGAGGGC CCCGCGTGGG GAGAGCTTGG AGCCCCCGGC CCCCCAAAGC 
ACCAAGCAGA GCCGGTCACC CACCCTGAGC TGTCGCCTCG CCGTCCTGAG AGAGAGCCCC 
AGGGCCCTCG CAGCCCAGAA GACCTCCCAC CTGGAAGACA CGGGGTCCGA CTCCAGCACC 
CCACTCAGCA CTCACGCCCC GGCTCCCCCG GCAAGCTTTC CCACCAAGAA ACCTCCCAGC 
CCCGAGCCCA AGCACAGCGA GCTCCTGGTC AGTGTCGGCA GCATCACCTC CGACCACGCC 
GGCACACCGT CTGCTCCCTA CCTGGCCGGC CTCGACTCCA GCCGGCTGAG CCCCGAGGTG 
CAGTCCATGG CCGAGAGCCG GGGATTTGAA GCTGATGACG AGAGGAGCGA GCTGGTCAGC 
GAGGGCCGGC CAGTGGAGAC AGACAGCGAG AGCGACTTTC CGGTCTTCCC CGCCGCCCCG 
GCCTCGGACA GGCTCTTCCG AGGAAAACTC CAAGAGGCCG CGAGGACCAG CCGGAGAAAC 
TCAGAAGGCA GTGAGGTCAG CTGCACGGAA GGAAGTTTAA CACCAAGTTT AGAAAGCCGG 
AGACAGCTCT TCAGCTCCCA TAAACTGATC GAGTGTGACA CTCTGTCCAG GAAGAAATCC 
GCCAGGTTCA AGTCAGACAG TGGGAGTCTA GGAGATGCCA AGAATGAGAA AGAAGCCCCT 
TCCATCACCA AAGTGTTCGA CGTTATGAAA AAAGGAAAAT CCACCGGAAA TTTACTGACA 
CCACCAGCCA GAAGCGAATC GGACAAACAG GAACCCACTT GGAAAACAAA AATAGCCGAT 
CGGTTAAAAC TGAGGCCCAA AGCCCCGGCC GATGACATGT TTGGAGTAGG AAGTCAAAAA 
ACCATCGCCG AACCTGCCAA AAGGAAAAAC ATCAAACGCA GACACACGCT GGGTGGGCAC 
AGGGATGCTA CTGAAATGAG TGTTCTGAAT TTCTGGAAAG CCCAGGAGCA CAGCGGGGAC 
AGAGAATCCG AACTTTCAGC TGTGAATCGA TTGAAGCCCA AATGCTCCGC CCAGGACCTG 
TCCATCTCAG ACTGGCTGGC CAGGGAGCGG CTACGCACCA GTATGACTGA CCTGAGCAGA 
GGGGACACGG GGGACCCCCG GCCTGAGAGT CTTGGCACCT TAGAAATACC AACCAGGGAC 
CCGCCCCTGT CTTTCCAGTC TGATGCAGAC AGTTCTTCCA GCACCTTGGC TTCAACTAAC 
AGGGCCCCTC TTTCCACACC ATCACAGTCA CCTGACCAAA TAAATGGAGA AAGCTTCCAG 
AACACGAGCC AAAACTCCAG TTCTGCAGCC AGGGTCCAAC CTCATCAACT GTCTGAAACC 
CCAGATCACA AAGCACAGTT CCATCCCTGT CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ARHGAP21
Entrez Gene ID	538584
Full Name	Rho GTPase activating protein 21
General protein information	Preferred Names Rho GTPase activating protein 21 Names rho GTPase-activating protein 21
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	ARHGAP21	XP_001100413.2
Bos taurus (cattle)	ARHGAP21	XP_005214355.1
Gallus gallus (chicken)	ARHGAP21	NP_001170828.1
Canis lupus familiaris (dog)	ARHGAP21	XP_005617050.1
Mus musculus (house mouse)	Arhgap21	NP_001121556.2
Danio rerio (zebrafish)	arhgap21b	XP_005163349.1
Pan troglodytes (chimpanzee)	ARHGAP21	XP_507699.3
Xenopus tropicalis (tropical clawed frog)	arhgap21	NP_001090761.1
Homo sapiens (human)	ARHGAP21	NP_065875.3
Rattus norvegicus (Norway rat)	Arhgap21	NP_001178622.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ARHGAP21 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

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