CDC42BPA cDNA ORF clone, Bos taurus(cattle)

The following CDC42BPA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDC42BPA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa239064 NM_001192937.2
Latest version!
Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa223662 XM_024976354.1
Latest version!
Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa223663 XM_024976355.1
Latest version!
Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa223664 XM_024976357.1
Latest version!
Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa223665 XM_024976358.1
Latest version!
Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa223666 XM_024976359.1
Latest version!
Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa223667 XM_024976360.1
Latest version!
Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa223668 XM_024976361.1
Latest version!
Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa223669 XM_024976362.1
Latest version!
Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa223670 XM_024976363.1
Latest version!
Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa37050 NM_001192937.1
Latest version!
Bos taurus CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa239064
    Clone ID Related Accession (Same CDS sequence) NM_001192937.2
    Accession Version NM_001192937.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5160bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase MRCK alpha
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000016.1. On May 30, 2018 this sequence version replaced NM_001192937.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA
    ACCTTAAGGA AAAAGGGATG CCCTGGTTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT
    CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAAGTGTC ATCAGTGTAC ATCTTTGATG
    GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT
    TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG
    GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG
    CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC
    TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG
    ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT
    GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT
    AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAGTGGGTG
    GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC
    TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT
    GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT
    GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC
    ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT
    CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC
    TGTCAAACAA TAACTTCTGG AAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT
    ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT
    AAGGAAATTC AAGTCCCATA CAACGTCCAG TGGATGGCAA TCTTCAGTGA GCAACTCTGT
    GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT
    ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC
    TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC
    ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC
    TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAATGCAGT TGACATCTTT
    GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC
    AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT
    AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CAGATAATAG TCGGAAGCAA
    ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA
    ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAGTT AATTTCTAAC
    CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA
    GACCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCCGTCAGT
    ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC
    CTATCAGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC
    AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCTTCGGGA
    GGCATGGACC AGGGAAGTGA CGCCCCAGCA CGGGACTATG ACGGAGAGGA CTCTGATTCT
    CCGCGGCATT CCACGGCTTC TAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCAGTGTCG
    CCCCGGAAGA CCAAGAGCCT ATCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179866.2
    CDS263..5422
    Translation

    Target ORF information:

    RefSeq Version NM_001192937.2
    Organism Bos taurus(cattle)
    Definition Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192937.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA
    ACCTTAAGGA AAAAGGGATG CCCTGGTTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT
    CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAAGTGTC ATCAGTGTAC ATCTTTGATG
    GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT
    TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG
    GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG
    CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC
    TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG
    ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT
    GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT
    AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAGTGGGTG
    GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC
    TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT
    GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT
    GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC
    ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT
    CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC
    TGTCAAACAA TAACTTCTGG AAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT
    ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT
    AAGGAAATTC AAGTCCCATA CAACGTCCAG TGGATGGCAA TCTTCAGTGA GCAACTCTGT
    GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT
    ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC
    TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC
    ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC
    TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAATGCAGT TGACATCTTT
    GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC
    AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT
    AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CAGATAATAG TCGGAAGCAA
    ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA
    ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAGTT AATTTCTAAC
    CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA
    GACCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCCGTCAGT
    ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC
    CTATCAGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC
    AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCTTCGGGA
    GGCATGGACC AGGGAAGTGA CGCCCCAGCA CGGGACTATG ACGGAGAGGA CTCTGATTCT
    CCGCGGCATT CCACGGCTTC TAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCAGTGTCG
    CCCCGGAAGA CCAAGAGCCT ATCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223662
    Clone ID Related Accession (Same CDS sequence) XM_024976354.1
    Accession Version XM_024976354.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase MRCK alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA
    CGTACTGATC CCGTTGAGAA CACATATGTA CGGAATCCGA ACGTCAAGTT TTACATCCAG
    TCAAGGTCCA CCTCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCT
    ACTCCACTTC CAGTTCATAC ACCAACCTTA AGGAAAAAGG GATGCCCTGG TTCAACTGGC
    TTTCCATCGA AGCGCAAGAT TCATCAGTTT TTTGTCAAAT CTTTTACTAC TCCCACCAAG
    TGTCATCAGT GTACATCTTT GATGGTGGGT TTGATAAGAC AAGGCTGTTC TTGTGAAGTG
    TGTGGATTTT CATGTCACAT AACTTGTGTA AACAAAGCTC CAACTGCTTG TCCAGTTCCT
    CCTGAACAGA CAAAAGGTCC TCTGGGTATA GATCCACAGA AAGGAATAGG AACAGCATAT
    GAAGGGCATG TCAGGATCCC TAAGCCAGCT GGAGTGAAGA AAGGATGGCA AAGAGCACTG
    GCTGTAGTCT GTGACTTCAA GCTCTTTCTG TACGATATTG CCGAAGGAAA GGCATCTCAG
    CCCAGTGTTG TAGTCAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA
    GTCTTGGCTT CTGATGTTAT TCATGCAAGT CGAAAAGATA TACCCTGTAT ATTTAGAGTC
    ACAGCTTCCC AGCTCTCAGC ATCTAGTAAC AAATGTTCCA TCCTGATGCT AGCTGATAGT
    GAAAATGAGA AGAGTAAGTG GGTGGGAGTA CTGAGTGAAT TGCACAAGAT TTTGAAGAAA
    AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTGCCC
    CTCATTAAAA CAACCCAGGC AGCTGCAATC ATAGATCATG AAAGAATAGC TTTGGGGAAT
    GAAGAAGGGT TATTTGTTGT GCATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT
    AAGAAGATTC ATCAGATAGA ACTCATTCCA AATGATCAAC TTGTTGCCGT GATCTCAGGA
    CGAAATCGAC ACGTCCGACT TTTTCCTATG TCAGCTCTGG ACGGGCGAGA GACTGATTTT
    TATAAGCTGG CAGAAACTAA AGGCTGTCAA ACAATAACTT CTGGAAAAGT GCGTCATGGA
    GCTCTTACAT GCTTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG
    AGCAAGACGC GTCACAGAAA ATTTAAGGAA ATTCAAGTCC CATACAACGT CCAGTGGATG
    GCAATCTTCA GTGAGCAACT CTGTGTGGGA TTCCAGTCTG GATTTCTAAG ATATCCCCTG
    AATGGAGAAG GAAGTCCATA CAGTATGCTC CATTCAAATG ACAATACACT CTCATTTATT
    GCACATCAAC CAATGGATGC CATCTGTGCG GTTGAAATCT CCAGTAAAGA ATACCTGCTG
    TGTTTTAACA GCATTGGAAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA
    CTGATGTGGC CAGCGAATCC TTCCTCTTGT TGTTACAATG CACCGTATCT CTCAGTGTAC
    AGTGAAAATG CAGTTGACAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTCCCA
    CTCAAAAAGG TTCGCCCCTT AAACAGTGAA GGATCATTAA ATCTTTTAGG CTTGGAGACG
    ATTAGATTGA TATATTTCAA AAATAAAATG GCAGAAGGGG ATGAACTGGT AGTTCCTGAA
    ACATCAGATA ATAGTCGGAA GCAAATGGTT AGAAATATTA ACAATAAGAG GCGTTACTCT
    TTCAGAGTTC CGGAAGAGGA AAGAATGCAA CAGAGGAGGG AGATGTTACG AGATCCAGAA
    ATGAGAAATA AGTTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA TATGGGTCCA
    GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGCCGG GTTTCCCTTA TCCATCCCCT
    CATCATCACT CCGGTCTGAT CTCCTCTCCG ATCAACTTTG AGCACATCTA TCACATGACT
    GTTAATTCTG CTGAAAAATT TCTCTCTCCT GATTCCATAA ACCCTGAATA TTCCCCGTCT
    CTGCGCTCTG TCCCCGGTAC ACCAAGCTTT ATGACTCTGA GGAACCCTCG GCCTCAGGAA
    AGTCGGACAG CATTCAGTGG CTCCGTCAGT ATTCCGTCTA TCACCAAATC CCGCCCTGAA
    CCCGGTCGCT CCATGAGCGC CAGCAGTGGC CTATCAGCAA GGTCATCTGC ACAGAATGGC
    AGTGCGTTAA AGAGAGAATT CTCTGGAGGC AGCTACAGTG CAAAGCGGCA GCCCATGCCC
    TCCCCATCGG AGGGCTCGCT GTCTTCGGGA GGCATGGACC AGGGAAGTGA CGCCCCAGCA
    CGGGACTATG ACGGAGAGGA CTCTGATTCT CCGCGGCATT CCACGGCTTC TAACAGTTCC
    AACCTGAGCA GTCCTCCCAG CCCAGTGTCG CCCCGGAAGA CCAAGAGCCT ATCTCTGGAG
    AGCACAGACC GCGGGAGCTG GGACCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832122.1
    CDS1282..6771
    Translation

    Target ORF information:

    RefSeq Version XM_024976354.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976354.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA
    CGTACTGATC CCGTTGAGAA CACATATGTA CGGAATCCGA ACGTCAAGTT TTACATCCAG
    TCAAGGTCCA CCTCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCT
    ACTCCACTTC CAGTTCATAC ACCAACCTTA AGGAAAAAGG GATGCCCTGG TTCAACTGGC
    TTTCCATCGA AGCGCAAGAT TCATCAGTTT TTTGTCAAAT CTTTTACTAC TCCCACCAAG
    TGTCATCAGT GTACATCTTT GATGGTGGGT TTGATAAGAC AAGGCTGTTC TTGTGAAGTG
    TGTGGATTTT CATGTCACAT AACTTGTGTA AACAAAGCTC CAACTGCTTG TCCAGTTCCT
    CCTGAACAGA CAAAAGGTCC TCTGGGTATA GATCCACAGA AAGGAATAGG AACAGCATAT
    GAAGGGCATG TCAGGATCCC TAAGCCAGCT GGAGTGAAGA AAGGATGGCA AAGAGCACTG
    GCTGTAGTCT GTGACTTCAA GCTCTTTCTG TACGATATTG CCGAAGGAAA GGCATCTCAG
    CCCAGTGTTG TAGTCAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA
    GTCTTGGCTT CTGATGTTAT TCATGCAAGT CGAAAAGATA TACCCTGTAT ATTTAGAGTC
    ACAGCTTCCC AGCTCTCAGC ATCTAGTAAC AAATGTTCCA TCCTGATGCT AGCTGATAGT
    GAAAATGAGA AGAGTAAGTG GGTGGGAGTA CTGAGTGAAT TGCACAAGAT TTTGAAGAAA
    AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTGCCC
    CTCATTAAAA CAACCCAGGC AGCTGCAATC ATAGATCATG AAAGAATAGC TTTGGGGAAT
    GAAGAAGGGT TATTTGTTGT GCATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT
    AAGAAGATTC ATCAGATAGA ACTCATTCCA AATGATCAAC TTGTTGCCGT GATCTCAGGA
    CGAAATCGAC ACGTCCGACT TTTTCCTATG TCAGCTCTGG ACGGGCGAGA GACTGATTTT
    TATAAGCTGG CAGAAACTAA AGGCTGTCAA ACAATAACTT CTGGAAAAGT GCGTCATGGA
    GCTCTTACAT GCTTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG
    AGCAAGACGC GTCACAGAAA ATTTAAGGAA ATTCAAGTCC CATACAACGT CCAGTGGATG
    GCAATCTTCA GTGAGCAACT CTGTGTGGGA TTCCAGTCTG GATTTCTAAG ATATCCCCTG
    AATGGAGAAG GAAGTCCATA CAGTATGCTC CATTCAAATG ACAATACACT CTCATTTATT
    GCACATCAAC CAATGGATGC CATCTGTGCG GTTGAAATCT CCAGTAAAGA ATACCTGCTG
    TGTTTTAACA GCATTGGAAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA
    CTGATGTGGC CAGCGAATCC TTCCTCTTGT TGTTACAATG CACCGTATCT CTCAGTGTAC
    AGTGAAAATG CAGTTGACAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTCCCA
    CTCAAAAAGG TTCGCCCCTT AAACAGTGAA GGATCATTAA ATCTTTTAGG CTTGGAGACG
    ATTAGATTGA TATATTTCAA AAATAAAATG GCAGAAGGGG ATGAACTGGT AGTTCCTGAA
    ACATCAGATA ATAGTCGGAA GCAAATGGTT AGAAATATTA ACAATAAGAG GCGTTACTCT
    TTCAGAGTTC CGGAAGAGGA AAGAATGCAA CAGAGGAGGG AGATGTTACG AGATCCAGAA
    ATGAGAAATA AGTTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA TATGGGTCCA
    GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGCCGG GTTTCCCTTA TCCATCCCCT
    CATCATCACT CCGGTCTGAT CTCCTCTCCG ATCAACTTTG AGCACATCTA TCACATGACT
    GTTAATTCTG CTGAAAAATT TCTCTCTCCT GATTCCATAA ACCCTGAATA TTCCCCGTCT
    CTGCGCTCTG TCCCCGGTAC ACCAAGCTTT ATGACTCTGA GGAACCCTCG GCCTCAGGAA
    AGTCGGACAG CATTCAGTGG CTCCGTCAGT ATTCCGTCTA TCACCAAATC CCGCCCTGAA
    CCCGGTCGCT CCATGAGCGC CAGCAGTGGC CTATCAGCAA GGTCATCTGC ACAGAATGGC
    AGTGCGTTAA AGAGAGAATT CTCTGGAGGC AGCTACAGTG CAAAGCGGCA GCCCATGCCC
    TCCCCATCGG AGGGCTCGCT GTCTTCGGGA GGCATGGACC AGGGAAGTGA CGCCCCAGCA
    CGGGACTATG ACGGAGAGGA CTCTGATTCT CCGCGGCATT CCACGGCTTC TAACAGTTCC
    AACCTGAGCA GTCCTCCCAG CCCAGTGTCG CCCCGGAAGA CCAAGAGCCT ATCTCTGGAG
    AGCACAGACC GCGGGAGCTG GGACCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223663
    Clone ID Related Accession (Same CDS sequence) XM_024976355.1
    Accession Version XM_024976355.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase MRCK alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ACGGAATCCG
    AACGTCAAGT TTTACATCCA GTCAAGGTCC ACCTCTCCTT CCACATCTAG TGAAGCTGAG
    CCAGTTAAGA CTGTAGACTC TACTCCACTT CCAGTTCATA CACCAACCTT AAGGAAAAAG
    GGATGCCCTG GTTCAACTGG CTTTCCATCG AAGCGCAAGA TTCATCAGTT TTTTGTCAAA
    TCTTTTACTA CTCCCACCAA GTGTCATCAG TGTACATCTT TGATGGTGGG TTTGATAAGA
    CAAGGCTGTT CTTGTGAAGT GTGTGGATTT TCATGTCACA TAACTTGTGT AAACAAAGCT
    CCAACTGCTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CTCTGGGTAT AGATCCACAG
    AAAGGAATAG GAACAGCATA TGAAGGGCAT GTCAGGATCC CTAAGCCAGC TGGAGTGAAG
    AAAGGATGGC AAAGAGCACT GGCTGTAGTC TGTGACTTCA AGCTCTTTCT GTACGATATT
    GCCGAAGGAA AGGCATCTCA GCCCAGTGTT GTAGTCAGTC AAGTGATTGA CATGAGGGAT
    GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT
    ATACCCTGTA TATTTAGAGT CACAGCTTCC CAGCTCTCAG CATCTAGTAA CAAATGTTCC
    ATCCTGATGC TAGCTGATAG TGAAAATGAG AAGAGTAAGT GGGTGGGAGT ACTGAGTGAA
    TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG
    GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAT
    GAAAGAATAG CTTTGGGGAA TGAAGAAGGG TTATTTGTTG TGCATGTCAC CAAAGATGAA
    ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATAG AACTCATTCC AAATGATCAA
    CTTGTTGCCG TGATCTCAGG ACGAAATCGA CACGTCCGAC TTTTTCCTAT GTCAGCTCTG
    GACGGGCGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGCTGTCA AACAATAACT
    TCTGGAAAAG TGCGTCATGG AGCTCTTACA TGCTTGTGTG TGGCTATGAA AAGGCAGGTC
    CTCTGTTATG AACTATTTCA GAGCAAGACG CGTCACAGAA AATTTAAGGA AATTCAAGTC
    CCATACAACG TCCAGTGGAT GGCAATCTTC AGTGAGCAAC TCTGTGTGGG ATTCCAGTCT
    GGATTTCTAA GATATCCCCT GAATGGAGAA GGAAGTCCAT ACAGTATGCT CCATTCAAAT
    GACAATACAC TCTCATTTAT TGCACATCAA CCAATGGATG CCATCTGTGC GGTTGAAATC
    TCCAGTAAAG AATACCTGCT GTGTTTTAAC AGCATTGGAA TATACACTGA CTGCCAGGGC
    CGAAGATCTA GACAACAGGA ACTGATGTGG CCAGCGAATC CTTCCTCTTG TTGTTACAAT
    GCACCGTATC TCTCAGTGTA CAGTGAAAAT GCAGTTGACA TCTTTGATGT GAACTCCATG
    GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGCCCCT TAAACAGTGA AGGATCATTA
    AATCTTTTAG GCTTGGAGAC GATTAGATTG ATATATTTCA AAAATAAAAT GGCAGAAGGG
    GATGAACTGG TAGTTCCTGA AACATCAGAT AATAGTCGGA AGCAAATGGT TAGAAATATT
    AACAATAAGA GGCGTTACTC TTTCAGAGTT CCGGAAGAGG AAAGAATGCA ACAGAGGAGG
    GAGATGTTAC GAGATCCAGA AATGAGAAAT AAGTTAATTT CTAACCCAAC TAATTTTAAC
    CACATAGCAC ATATGGGTCC AGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCG
    GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT
    GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA
    AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCCGGTA CACCAAGCTT TATGACTCTG
    AGGAACCCTC GGCCTCAGGA AAGTCGGACA GCATTCAGTG GCTCCGTCAG TATTCCGTCT
    ATCACCAAAT CCCGCCCTGA ACCCGGTCGC TCCATGAGCG CCAGCAGTGG CCTATCAGCA
    AGGTCATCTG CACAGAATGG CAGTGCGTTA AAGAGAGAAT TCTCTGGAGG CAGCTACAGT
    GCAAAGCGGC AGCCCATGCC CTCCCCATCG GAGGGCTCGC TGTCTTCGGG AGGCATGGAC
    CAGGGAAGTG ACGCCCCAGC ACGGGACTAT GACGGAGAGG ACTCTGATTC TCCGCGGCAT
    TCCACGGCTT CTAACAGTTC CAACCTGAGC AGTCCTCCCA GCCCAGTGTC GCCCCGGAAG
    ACCAAGAGCC TATCTCTGGA GAGCACAGAC CGCGGGAGCT GGGACCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832123.1
    CDS1282..6732
    Translation

    Target ORF information:

    RefSeq Version XM_024976355.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976355.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ACGGAATCCG
    AACGTCAAGT TTTACATCCA GTCAAGGTCC ACCTCTCCTT CCACATCTAG TGAAGCTGAG
    CCAGTTAAGA CTGTAGACTC TACTCCACTT CCAGTTCATA CACCAACCTT AAGGAAAAAG
    GGATGCCCTG GTTCAACTGG CTTTCCATCG AAGCGCAAGA TTCATCAGTT TTTTGTCAAA
    TCTTTTACTA CTCCCACCAA GTGTCATCAG TGTACATCTT TGATGGTGGG TTTGATAAGA
    CAAGGCTGTT CTTGTGAAGT GTGTGGATTT TCATGTCACA TAACTTGTGT AAACAAAGCT
    CCAACTGCTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CTCTGGGTAT AGATCCACAG
    AAAGGAATAG GAACAGCATA TGAAGGGCAT GTCAGGATCC CTAAGCCAGC TGGAGTGAAG
    AAAGGATGGC AAAGAGCACT GGCTGTAGTC TGTGACTTCA AGCTCTTTCT GTACGATATT
    GCCGAAGGAA AGGCATCTCA GCCCAGTGTT GTAGTCAGTC AAGTGATTGA CATGAGGGAT
    GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT
    ATACCCTGTA TATTTAGAGT CACAGCTTCC CAGCTCTCAG CATCTAGTAA CAAATGTTCC
    ATCCTGATGC TAGCTGATAG TGAAAATGAG AAGAGTAAGT GGGTGGGAGT ACTGAGTGAA
    TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG
    GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAT
    GAAAGAATAG CTTTGGGGAA TGAAGAAGGG TTATTTGTTG TGCATGTCAC CAAAGATGAA
    ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATAG AACTCATTCC AAATGATCAA
    CTTGTTGCCG TGATCTCAGG ACGAAATCGA CACGTCCGAC TTTTTCCTAT GTCAGCTCTG
    GACGGGCGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGCTGTCA AACAATAACT
    TCTGGAAAAG TGCGTCATGG AGCTCTTACA TGCTTGTGTG TGGCTATGAA AAGGCAGGTC
    CTCTGTTATG AACTATTTCA GAGCAAGACG CGTCACAGAA AATTTAAGGA AATTCAAGTC
    CCATACAACG TCCAGTGGAT GGCAATCTTC AGTGAGCAAC TCTGTGTGGG ATTCCAGTCT
    GGATTTCTAA GATATCCCCT GAATGGAGAA GGAAGTCCAT ACAGTATGCT CCATTCAAAT
    GACAATACAC TCTCATTTAT TGCACATCAA CCAATGGATG CCATCTGTGC GGTTGAAATC
    TCCAGTAAAG AATACCTGCT GTGTTTTAAC AGCATTGGAA TATACACTGA CTGCCAGGGC
    CGAAGATCTA GACAACAGGA ACTGATGTGG CCAGCGAATC CTTCCTCTTG TTGTTACAAT
    GCACCGTATC TCTCAGTGTA CAGTGAAAAT GCAGTTGACA TCTTTGATGT GAACTCCATG
    GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGCCCCT TAAACAGTGA AGGATCATTA
    AATCTTTTAG GCTTGGAGAC GATTAGATTG ATATATTTCA AAAATAAAAT GGCAGAAGGG
    GATGAACTGG TAGTTCCTGA AACATCAGAT AATAGTCGGA AGCAAATGGT TAGAAATATT
    AACAATAAGA GGCGTTACTC TTTCAGAGTT CCGGAAGAGG AAAGAATGCA ACAGAGGAGG
    GAGATGTTAC GAGATCCAGA AATGAGAAAT AAGTTAATTT CTAACCCAAC TAATTTTAAC
    CACATAGCAC ATATGGGTCC AGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCG
    GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT
    GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA
    AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCCGGTA CACCAAGCTT TATGACTCTG
    AGGAACCCTC GGCCTCAGGA AAGTCGGACA GCATTCAGTG GCTCCGTCAG TATTCCGTCT
    ATCACCAAAT CCCGCCCTGA ACCCGGTCGC TCCATGAGCG CCAGCAGTGG CCTATCAGCA
    AGGTCATCTG CACAGAATGG CAGTGCGTTA AAGAGAGAAT TCTCTGGAGG CAGCTACAGT
    GCAAAGCGGC AGCCCATGCC CTCCCCATCG GAGGGCTCGC TGTCTTCGGG AGGCATGGAC
    CAGGGAAGTG ACGCCCCAGC ACGGGACTAT GACGGAGAGG ACTCTGATTC TCCGCGGCAT
    TCCACGGCTT CTAACAGTTC CAACCTGAGC AGTCCTCCCA GCCCAGTGTC GCCCCGGAAG
    ACCAAGAGCC TATCTCTGGA GAGCACAGAC CGCGGGAGCT GGGACCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223664
    Clone ID Related Accession (Same CDS sequence) XM_024976357.1
    Accession Version XM_024976357.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5394bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase MRCK alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT
    TTTTTGGATG ACACTGTAGA CTCTACTCCA CTTCCAGTTC ATACACCAAC CTTAAGGAAA
    AAGGGATGCC CTGGTTCAAC TGGCTTTCCA TCGAAGCGCA AGATTCATCA GTTTTTTGTC
    AAATCTTTTA CTACTCCCAC CAAGTGTCAT CAGTGTACAT CTTTGATGGT GGGTTTGATA
    AGACAAGGCT GTTCTTGTGA AGTGTGTGGA TTTTCATGTC ACATAACTTG TGTAAACAAA
    GCTCCAACTG CTTGTCCAGT TCCTCCTGAA CAGACAAAAG GTCCTCTGGG TATAGATCCA
    CAGAAAGGAA TAGGAACAGC ATATGAAGGG CATGTCAGGA TCCCTAAGCC AGCTGGAGTG
    AAGAAAGGAT GGCAAAGAGC ACTGGCTGTA GTCTGTGACT TCAAGCTCTT TCTGTACGAT
    ATTGCCGAAG GAAAGGCATC TCAGCCCAGT GTTGTAGTCA GTCAAGTGAT TGACATGAGG
    GATGAAGAAT TTTCTGTGAG TTCAGTCTTG GCTTCTGATG TTATTCATGC AAGTCGAAAA
    GATATACCCT GTATATTTAG AGTCACAGCT TCCCAGCTCT CAGCATCTAG TAACAAATGT
    TCCATCCTGA TGCTAGCTGA TAGTGAAAAT GAGAAGAGTA AGTGGGTGGG AGTACTGAGT
    GAATTGCACA AGATTTTGAA GAAAAACAAA TTCAGAGACC GCTCAGTCTA TGTTCCCAAA
    GAGGCTTATG ACAGCACTCT GCCCCTCATT AAAACAACCC AGGCAGCTGC AATCATAGAT
    CATGAAAGAA TAGCTTTGGG GAATGAAGAA GGGTTATTTG TTGTGCATGT CACCAAAGAT
    GAAATTATTA GAGTTGGTGA CAATAAGAAG ATTCATCAGA TAGAACTCAT TCCAAATGAT
    CAACTTGTTG CCGTGATCTC AGGACGAAAT CGACACGTCC GACTTTTTCC TATGTCAGCT
    CTGGACGGGC GAGAGACTGA TTTTTATAAG CTGGCAGAAA CTAAAGGCTG TCAAACAATA
    ACTTCTGGAA AAGTGCGTCA TGGAGCTCTT ACATGCTTGT GTGTGGCTAT GAAAAGGCAG
    GTCCTCTGTT ATGAACTATT TCAGAGCAAG ACGCGTCACA GAAAATTTAA GGAAATTCAA
    GTCCCATACA ACGTCCAGTG GATGGCAATC TTCAGTGAGC AACTCTGTGT GGGATTCCAG
    TCTGGATTTC TAAGATATCC CCTGAATGGA GAAGGAAGTC CATACAGTAT GCTCCATTCA
    AATGACAATA CACTCTCATT TATTGCACAT CAACCAATGG ATGCCATCTG TGCGGTTGAA
    ATCTCCAGTA AAGAATACCT GCTGTGTTTT AACAGCATTG GAATATACAC TGACTGCCAG
    GGCCGAAGAT CTAGACAACA GGAACTGATG TGGCCAGCGA ATCCTTCCTC TTGTTGTTAC
    AATGCACCGT ATCTCTCAGT GTACAGTGAA AATGCAGTTG ACATCTTTGA TGTGAACTCC
    ATGGAATGGA TTCAGACTCT CCCACTCAAA AAGGTTCGCC CCTTAAACAG TGAAGGATCA
    TTAAATCTTT TAGGCTTGGA GACGATTAGA TTGATATATT TCAAAAATAA AATGGCAGAA
    GGGGATGAAC TGGTAGTTCC TGAAACATCA GATAATAGTC GGAAGCAAAT GGTTAGAAAT
    ATTAACAATA AGAGGCGTTA CTCTTTCAGA GTTCCGGAAG AGGAAAGAAT GCAACAGAGG
    AGGGAGATGT TACGAGATCC AGAAATGAGA AATAAGTTAA TTTCTAACCC AACTAATTTT
    AACCACATAG CACATATGGG TCCAGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG
    CCGGGTTTCC CTTATCCATC CCCTCATCAT CACTCCGGTC TGATCTCCTC TCCGATCAAC
    TTTGAGCACA TCTATCACAT GACTGTTAAT TCTGCTGAAA AATTTCTCTC TCCTGATTCC
    ATAAACCCTG AATATTCCCC GTCTCTGCGC TCTGTCCCCG GTACACCAAG CTTTATGACT
    CTGAGGAACC CTCGGCCTCA GGAAAGTCGG ACAGCATTCA GTGGCTCCGT CAGTATTCCG
    TCTATCACCA AATCCCGCCC TGAACCCGGT CGCTCCATGA GCGCCAGCAG TGGCCTATCA
    GCAAGGTCAT CTGCACAGAA TGGCAGTGCG TTAAAGAGAG AATTCTCTGG AGGCAGCTAC
    AGTGCAAAGC GGCAGCCCAT GCCCTCCCCA TCGGAGGGCT CGCTGTCTTC GGGAGGCATG
    GACCAGGGAA GTGACGCCCC AGCACGGGAC TATGACGGAG AGGACTCTGA TTCTCCGCGG
    CATTCCACGG CTTCTAACAG TTCCAACCTG AGCAGTCCTC CCAGCCCAGT GTCGCCCCGG
    AAGACCAAGA GCCTATCTCT GGAGAGCACA GACCGCGGGA GCTGGGACCC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832125.1
    CDS1282..6675
    Translation

    Target ORF information:

    RefSeq Version XM_024976357.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976357.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT
    TTTTTGGATG ACACTGTAGA CTCTACTCCA CTTCCAGTTC ATACACCAAC CTTAAGGAAA
    AAGGGATGCC CTGGTTCAAC TGGCTTTCCA TCGAAGCGCA AGATTCATCA GTTTTTTGTC
    AAATCTTTTA CTACTCCCAC CAAGTGTCAT CAGTGTACAT CTTTGATGGT GGGTTTGATA
    AGACAAGGCT GTTCTTGTGA AGTGTGTGGA TTTTCATGTC ACATAACTTG TGTAAACAAA
    GCTCCAACTG CTTGTCCAGT TCCTCCTGAA CAGACAAAAG GTCCTCTGGG TATAGATCCA
    CAGAAAGGAA TAGGAACAGC ATATGAAGGG CATGTCAGGA TCCCTAAGCC AGCTGGAGTG
    AAGAAAGGAT GGCAAAGAGC ACTGGCTGTA GTCTGTGACT TCAAGCTCTT TCTGTACGAT
    ATTGCCGAAG GAAAGGCATC TCAGCCCAGT GTTGTAGTCA GTCAAGTGAT TGACATGAGG
    GATGAAGAAT TTTCTGTGAG TTCAGTCTTG GCTTCTGATG TTATTCATGC AAGTCGAAAA
    GATATACCCT GTATATTTAG AGTCACAGCT TCCCAGCTCT CAGCATCTAG TAACAAATGT
    TCCATCCTGA TGCTAGCTGA TAGTGAAAAT GAGAAGAGTA AGTGGGTGGG AGTACTGAGT
    GAATTGCACA AGATTTTGAA GAAAAACAAA TTCAGAGACC GCTCAGTCTA TGTTCCCAAA
    GAGGCTTATG ACAGCACTCT GCCCCTCATT AAAACAACCC AGGCAGCTGC AATCATAGAT
    CATGAAAGAA TAGCTTTGGG GAATGAAGAA GGGTTATTTG TTGTGCATGT CACCAAAGAT
    GAAATTATTA GAGTTGGTGA CAATAAGAAG ATTCATCAGA TAGAACTCAT TCCAAATGAT
    CAACTTGTTG CCGTGATCTC AGGACGAAAT CGACACGTCC GACTTTTTCC TATGTCAGCT
    CTGGACGGGC GAGAGACTGA TTTTTATAAG CTGGCAGAAA CTAAAGGCTG TCAAACAATA
    ACTTCTGGAA AAGTGCGTCA TGGAGCTCTT ACATGCTTGT GTGTGGCTAT GAAAAGGCAG
    GTCCTCTGTT ATGAACTATT TCAGAGCAAG ACGCGTCACA GAAAATTTAA GGAAATTCAA
    GTCCCATACA ACGTCCAGTG GATGGCAATC TTCAGTGAGC AACTCTGTGT GGGATTCCAG
    TCTGGATTTC TAAGATATCC CCTGAATGGA GAAGGAAGTC CATACAGTAT GCTCCATTCA
    AATGACAATA CACTCTCATT TATTGCACAT CAACCAATGG ATGCCATCTG TGCGGTTGAA
    ATCTCCAGTA AAGAATACCT GCTGTGTTTT AACAGCATTG GAATATACAC TGACTGCCAG
    GGCCGAAGAT CTAGACAACA GGAACTGATG TGGCCAGCGA ATCCTTCCTC TTGTTGTTAC
    AATGCACCGT ATCTCTCAGT GTACAGTGAA AATGCAGTTG ACATCTTTGA TGTGAACTCC
    ATGGAATGGA TTCAGACTCT CCCACTCAAA AAGGTTCGCC CCTTAAACAG TGAAGGATCA
    TTAAATCTTT TAGGCTTGGA GACGATTAGA TTGATATATT TCAAAAATAA AATGGCAGAA
    GGGGATGAAC TGGTAGTTCC TGAAACATCA GATAATAGTC GGAAGCAAAT GGTTAGAAAT
    ATTAACAATA AGAGGCGTTA CTCTTTCAGA GTTCCGGAAG AGGAAAGAAT GCAACAGAGG
    AGGGAGATGT TACGAGATCC AGAAATGAGA AATAAGTTAA TTTCTAACCC AACTAATTTT
    AACCACATAG CACATATGGG TCCAGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG
    CCGGGTTTCC CTTATCCATC CCCTCATCAT CACTCCGGTC TGATCTCCTC TCCGATCAAC
    TTTGAGCACA TCTATCACAT GACTGTTAAT TCTGCTGAAA AATTTCTCTC TCCTGATTCC
    ATAAACCCTG AATATTCCCC GTCTCTGCGC TCTGTCCCCG GTACACCAAG CTTTATGACT
    CTGAGGAACC CTCGGCCTCA GGAAAGTCGG ACAGCATTCA GTGGCTCCGT CAGTATTCCG
    TCTATCACCA AATCCCGCCC TGAACCCGGT CGCTCCATGA GCGCCAGCAG TGGCCTATCA
    GCAAGGTCAT CTGCACAGAA TGGCAGTGCG TTAAAGAGAG AATTCTCTGG AGGCAGCTAC
    AGTGCAAAGC GGCAGCCCAT GCCCTCCCCA TCGGAGGGCT CGCTGTCTTC GGGAGGCATG
    GACCAGGGAA GTGACGCCCC AGCACGGGAC TATGACGGAG AGGACTCTGA TTCTCCGCGG
    CATTCCACGG CTTCTAACAG TTCCAACCTG AGCAGTCCTC CCAGCCCAGT GTCGCCCCGG
    AAGACCAAGA GCCTATCTCT GGAGAGCACA GACCGCGGGA GCTGGGACCC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223665
    Clone ID Related Accession (Same CDS sequence) XM_024976358.1
    Accession Version XM_024976358.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase MRCK alpha isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA
    CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC
    CCTGGTTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT
    ACTACTCCCA CCAAGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC
    TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT
    GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA
    ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA
    TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA
    GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA
    TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC
    TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG
    ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAGTGGGTGG GAGTACTGAG TGAATTGCAC
    AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT
    GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA
    ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT
    AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT
    GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG
    CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGA
    AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT
    TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCATAC
    AACGTCCAGT GGATGGCAAT CTTCAGTGAG CAACTCTGTG TGGGATTCCA GTCTGGATTT
    CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT
    ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT
    AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA
    TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG
    TATCTCTCAG TGTACAGTGA AAATGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG
    ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT
    TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA
    CTGGTAGTTC CTGAAACATC AGATAATAGT CGGAAGCAAA TGGTTAGAAA TATTAACAAT
    AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG
    TTACGAGATC CAGAAATGAG AAATAAGTTA ATTTCTAACC CAACTAATTT TAACCACATA
    GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GCCGGGTTTC
    CCTTATCCAT CCCCTCATCA TCACTCCGGT CTGATCTCCT CTCCGATCAA CTTTGAGCAC
    ATCTATCACA TGACTGTTAA TTCTGCTGAA AAATTTCTCT CTCCTGATTC CATAAACCCT
    GAATATTCCC CGTCTCTGCG CTCTGTCCCC GGTACACCAA GCTTTATGAC TCTGAGGAAC
    CCTCGGCCTC AGGAAAGTCG GACAGCATTC AGTGGCTCCG TCAGTATTCC GTCTATCACC
    AAATCCCGCC CTGAACCCGG TCGCTCCATG AGCGCCAGCA GTGGCCTATC AGCAAGGTCA
    TCTGCACAGA ATGGCAGTGC GTTAAAGAGA GAATTCTCTG GAGGCAGCTA CAGTGCAAAG
    CGGCAGCCCA TGCCCTCCCC ATCGGAGGGC TCGCTGTCTT CGGGAGGCAT GGACCAGGGA
    AGTGACGCCC CAGCACGGGA CTATGACGGA GAGGACTCTG ATTCTCCGCG GCATTCCACG
    GCTTCTAACA GTTCCAACCT GAGCAGTCCT CCCAGCCCAG TGTCGCCCCG GAAGACCAAG
    AGCCTATCTC TGGAGAGCAC AGACCGCGGG AGCTGGGACC CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832126.1
    CDS1282..6666
    Translation

    Target ORF information:

    RefSeq Version XM_024976358.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976358.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA
    CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC
    CCTGGTTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT
    ACTACTCCCA CCAAGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC
    TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT
    GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA
    ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA
    TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA
    GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA
    TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC
    TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG
    ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAGTGGGTGG GAGTACTGAG TGAATTGCAC
    AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT
    GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA
    ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT
    AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT
    GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG
    CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGA
    AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT
    TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCATAC
    AACGTCCAGT GGATGGCAAT CTTCAGTGAG CAACTCTGTG TGGGATTCCA GTCTGGATTT
    CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT
    ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT
    AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA
    TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG
    TATCTCTCAG TGTACAGTGA AAATGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG
    ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT
    TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA
    CTGGTAGTTC CTGAAACATC AGATAATAGT CGGAAGCAAA TGGTTAGAAA TATTAACAAT
    AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG
    TTACGAGATC CAGAAATGAG AAATAAGTTA ATTTCTAACC CAACTAATTT TAACCACATA
    GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GCCGGGTTTC
    CCTTATCCAT CCCCTCATCA TCACTCCGGT CTGATCTCCT CTCCGATCAA CTTTGAGCAC
    ATCTATCACA TGACTGTTAA TTCTGCTGAA AAATTTCTCT CTCCTGATTC CATAAACCCT
    GAATATTCCC CGTCTCTGCG CTCTGTCCCC GGTACACCAA GCTTTATGAC TCTGAGGAAC
    CCTCGGCCTC AGGAAAGTCG GACAGCATTC AGTGGCTCCG TCAGTATTCC GTCTATCACC
    AAATCCCGCC CTGAACCCGG TCGCTCCATG AGCGCCAGCA GTGGCCTATC AGCAAGGTCA
    TCTGCACAGA ATGGCAGTGC GTTAAAGAGA GAATTCTCTG GAGGCAGCTA CAGTGCAAAG
    CGGCAGCCCA TGCCCTCCCC ATCGGAGGGC TCGCTGTCTT CGGGAGGCAT GGACCAGGGA
    AGTGACGCCC CAGCACGGGA CTATGACGGA GAGGACTCTG ATTCTCCGCG GCATTCCACG
    GCTTCTAACA GTTCCAACCT GAGCAGTCCT CCCAGCCCAG TGTCGCCCCG GAAGACCAAG
    AGCCTATCTC TGGAGAGCAC AGACCGCGGG AGCTGGGACC CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223666
    Clone ID Related Accession (Same CDS sequence) XM_024976359.1
    Accession Version XM_024976359.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase MRCK alpha isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA
    ACCTTAAGGA AAAAGGGATG CCCTGGTTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT
    CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAAGTGTC ATCAGTGTAC ATCTTTGATG
    GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT
    TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG
    GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG
    CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC
    TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG
    ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT
    GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT
    AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAGTGGGTG
    GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC
    TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT
    GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT
    GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC
    ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT
    CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC
    TGTCAAACAA TAACTTCTGG AAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT
    ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT
    AAGGAAATTC AAGTCCCATA CAACGTCCAG TGGATGGCAA TCTTCAGTGA GCAACTCTGT
    GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT
    ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC
    TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC
    ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC
    TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAATGCAGT TGACATCTTT
    GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC
    AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT
    AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CAGATAATAG TCGGAAGCAA
    ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA
    ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAGTT AATTTCTAAC
    CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA
    GACCTGCCCA TGCCGGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC
    TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC
    TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC CGGTACACCA
    AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGCATT CAGTGGCTCC
    GTCAGTATTC CGTCTATCAC CAAATCCCGC CCTGAACCCG GTCGCTCCAT GAGCGCCAGC
    AGTGGCCTAT CAGCAAGGTC ATCTGCACAG AATGGCAGTG CGTTAAAGAG AGAATTCTCT
    GGAGGCAGCT ACAGTGCAAA GCGGCAGCCC ATGCCCTCCC CATCGGAGGG CTCGCTGTCT
    TCGGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCACGGG ACTATGACGG AGAGGACTCT
    GATTCTCCGC GGCATTCCAC GGCTTCTAAC AGTTCCAACC TGAGCAGTCC TCCCAGCCCA
    GTGTCGCCCC GGAAGACCAA GAGCCTATCT CTGGAGAGCA CAGACCGCGG GAGCTGGGAC
    CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832127.1
    CDS1282..6627
    Translation

    Target ORF information:

    RefSeq Version XM_024976359.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976359.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA
    ACCTTAAGGA AAAAGGGATG CCCTGGTTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT
    CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAAGTGTC ATCAGTGTAC ATCTTTGATG
    GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT
    TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG
    GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG
    CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC
    TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG
    ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT
    GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT
    AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAGTGGGTG
    GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC
    TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT
    GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT
    GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC
    ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT
    CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC
    TGTCAAACAA TAACTTCTGG AAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT
    ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT
    AAGGAAATTC AAGTCCCATA CAACGTCCAG TGGATGGCAA TCTTCAGTGA GCAACTCTGT
    GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT
    ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC
    TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC
    ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC
    TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAATGCAGT TGACATCTTT
    GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC
    AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT
    AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CAGATAATAG TCGGAAGCAA
    ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA
    ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAGTT AATTTCTAAC
    CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA
    GACCTGCCCA TGCCGGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC
    TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC
    TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC CGGTACACCA
    AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGCATT CAGTGGCTCC
    GTCAGTATTC CGTCTATCAC CAAATCCCGC CCTGAACCCG GTCGCTCCAT GAGCGCCAGC
    AGTGGCCTAT CAGCAAGGTC ATCTGCACAG AATGGCAGTG CGTTAAAGAG AGAATTCTCT
    GGAGGCAGCT ACAGTGCAAA GCGGCAGCCC ATGCCCTCCC CATCGGAGGG CTCGCTGTCT
    TCGGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCACGGG ACTATGACGG AGAGGACTCT
    GATTCTCCGC GGCATTCCAC GGCTTCTAAC AGTTCCAACC TGAGCAGTCC TCCCAGCCCA
    GTGTCGCCCC GGAAGACCAA GAGCCTATCT CTGGAGAGCA CAGACCGCGG GAGCTGGGAC
    CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223667
    Clone ID Related Accession (Same CDS sequence) XM_024976360.1
    Accession Version XM_024976360.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase MRCK alpha isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA
    CGTACTGATC CCGTTGAGAA CACATATGTA CGGAATCCGA ACGTCAAGTT TTACATCCAG
    TCAAGGTCCA CCTCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCT
    ACTCCACTTC CAGTTCATAC ACCAACCTTA AGGAAAAAGG GATGCCCTGG TTCAACTGGC
    TTTCCATCGA AGCGCAAGAT TCATCAGTTT TTTGTCAAAT CTTTTACTAC TCCCACCAAG
    TGTCATCAGT GTACATCTTT GATGGTGGGT TTGATAAGAC AAGGCTGTTC TTGTGAAGTG
    TGTGGATTTT CATGTCACAT AACTTGTGTA AACAAAGCTC CAACTGCTTG TCCAGTTCCT
    CCTGAACAGA CAAAAGGTCC TCTGGGTATA GATCCACAGA AAGGAATAGG AACAGCATAT
    GAAGGGCATG TCAGGATCCC TAAGCCAGCT GGAGTGAAGA AAGGATGGCA AAGAGCACTG
    GCTGTAGTCT GTGACTTCAA GCTCTTTCTG TACGATATTG CCGAAGGAAA GGCATCTCAG
    CCCAGTGTTG TAGTCAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA
    GTCTTGGCTT CTGATGTTAT TCATGCAAGT CGAAAAGATA TACCCTGTAT ATTTAGAGTC
    ACAGCTTCCC AGCTCTCAGC ATCTAGTAAC AAATGTTCCA TCCTGATGCT AGCTGATAGT
    GAAAATGAGA AGAGTAAGTG GGTGGGAGTA CTGAGTGAAT TGCACAAGAT TTTGAAGAAA
    AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTGCCC
    CTCATTAAAA CAACCCAGGC AGCTGCAATC ATAGATCATG AAAGAATAGC TTTGGGGAAT
    GAAGAAGGGT TATTTGTTGT GCATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT
    AAGAAGATTC ATCAGATAGA ACTCATTCCA AATGATCAAC TTGTTGCCGT GATCTCAGGA
    CGAAATCGAC ACGTCCGACT TTTTCCTATG TCAGCTCTGG ACGGGCGAGA GACTGATTTT
    TATAAGCTGG CAGAAACTAA AGGCTGTCAA ACAATAACTT CTGGAAAAGT GCGTCATGGA
    GCTCTTACAT GCTTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG
    AGCAAGACGC GTCACAGAAA ATTTAAGGAA ATTCAAGTCC CATACAACGT CCAGTGGATG
    GCAATCTTCA GTGAGCAACT CTGTGTGGGA TTCCAGTCTG GATTTCTAAG ATATCCCCTG
    AATGGAGAAG GAAGTCCATA CAGTATGCTC CATTCAAATG ACAATACACT CTCATTTATT
    GCACATCAAC CAATGGATGC CATCTGTGCG GTTGAAATCT CCAGTAAAGA ATACCTGCTG
    TGTTTTAACA GCATTGGAAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA
    CTGATGTGGC CAGCGAATCC TTCCTCTTGT TGTTACAATG CACCGTATCT CTCAGTGTAC
    AGTGAAAATG CAGTTGACAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTCCCA
    CTCAAAAAGG TTCGCCCCTT AAACAGTGAA GGATCATTAA ATCTTTTAGG CTTGGAGACG
    ATTAGATTGA TATATTTCAA AAATAAAATG GCAGAAGGGG ATGAACTGGT AGTTCCTGAA
    ACATCAGATA ATAGTCGGAA GCAAATGGTT AGAAATATTA ACAATAAGAG GCGTTACTCT
    TTCAGAGTTC CGGAAGAGGA AAGAATGCAA CAGAGGAGGG AGATGTTACG AGATCCAGAA
    ATGAGAAATA AGTTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA TATGGGTCCA
    GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGAACC CTCGGCCTCA GGAAAGTCGG
    ACAGCATTCA GTGGCTCCGT CAGTATTCCG TCTATCACCA AATCCCGCCC TGAACCCGGT
    CGCTCCATGA GCGCCAGCAG TGGCCTATCA GCAAGGTCAT CTGCACAGAA TGGCAGTGCG
    TTAAAGAGAG AATTCTCTGG AGGCAGCTAC AGTGCAAAGC GGCAGCCCAT GCCCTCCCCA
    TCGGAGGGCT CGCTGTCTTC GGGAGGCATG GACCAGGGAA GTGACGCCCC AGCACGGGAC
    TATGACGGAG AGGACTCTGA TTCTCCGCGG CATTCCACGG CTTCTAACAG TTCCAACCTG
    AGCAGTCCTC CCAGCCCAGT GTCGCCCCGG AAGACCAAGA GCCTATCTCT GGAGAGCACA
    GACCGCGGGA GCTGGGACCC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832128.1
    CDS1282..6585
    Translation

    Target ORF information:

    RefSeq Version XM_024976360.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976360.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA
    CGTACTGATC CCGTTGAGAA CACATATGTA CGGAATCCGA ACGTCAAGTT TTACATCCAG
    TCAAGGTCCA CCTCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCT
    ACTCCACTTC CAGTTCATAC ACCAACCTTA AGGAAAAAGG GATGCCCTGG TTCAACTGGC
    TTTCCATCGA AGCGCAAGAT TCATCAGTTT TTTGTCAAAT CTTTTACTAC TCCCACCAAG
    TGTCATCAGT GTACATCTTT GATGGTGGGT TTGATAAGAC AAGGCTGTTC TTGTGAAGTG
    TGTGGATTTT CATGTCACAT AACTTGTGTA AACAAAGCTC CAACTGCTTG TCCAGTTCCT
    CCTGAACAGA CAAAAGGTCC TCTGGGTATA GATCCACAGA AAGGAATAGG AACAGCATAT
    GAAGGGCATG TCAGGATCCC TAAGCCAGCT GGAGTGAAGA AAGGATGGCA AAGAGCACTG
    GCTGTAGTCT GTGACTTCAA GCTCTTTCTG TACGATATTG CCGAAGGAAA GGCATCTCAG
    CCCAGTGTTG TAGTCAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA
    GTCTTGGCTT CTGATGTTAT TCATGCAAGT CGAAAAGATA TACCCTGTAT ATTTAGAGTC
    ACAGCTTCCC AGCTCTCAGC ATCTAGTAAC AAATGTTCCA TCCTGATGCT AGCTGATAGT
    GAAAATGAGA AGAGTAAGTG GGTGGGAGTA CTGAGTGAAT TGCACAAGAT TTTGAAGAAA
    AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTGCCC
    CTCATTAAAA CAACCCAGGC AGCTGCAATC ATAGATCATG AAAGAATAGC TTTGGGGAAT
    GAAGAAGGGT TATTTGTTGT GCATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT
    AAGAAGATTC ATCAGATAGA ACTCATTCCA AATGATCAAC TTGTTGCCGT GATCTCAGGA
    CGAAATCGAC ACGTCCGACT TTTTCCTATG TCAGCTCTGG ACGGGCGAGA GACTGATTTT
    TATAAGCTGG CAGAAACTAA AGGCTGTCAA ACAATAACTT CTGGAAAAGT GCGTCATGGA
    GCTCTTACAT GCTTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG
    AGCAAGACGC GTCACAGAAA ATTTAAGGAA ATTCAAGTCC CATACAACGT CCAGTGGATG
    GCAATCTTCA GTGAGCAACT CTGTGTGGGA TTCCAGTCTG GATTTCTAAG ATATCCCCTG
    AATGGAGAAG GAAGTCCATA CAGTATGCTC CATTCAAATG ACAATACACT CTCATTTATT
    GCACATCAAC CAATGGATGC CATCTGTGCG GTTGAAATCT CCAGTAAAGA ATACCTGCTG
    TGTTTTAACA GCATTGGAAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA
    CTGATGTGGC CAGCGAATCC TTCCTCTTGT TGTTACAATG CACCGTATCT CTCAGTGTAC
    AGTGAAAATG CAGTTGACAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTCCCA
    CTCAAAAAGG TTCGCCCCTT AAACAGTGAA GGATCATTAA ATCTTTTAGG CTTGGAGACG
    ATTAGATTGA TATATTTCAA AAATAAAATG GCAGAAGGGG ATGAACTGGT AGTTCCTGAA
    ACATCAGATA ATAGTCGGAA GCAAATGGTT AGAAATATTA ACAATAAGAG GCGTTACTCT
    TTCAGAGTTC CGGAAGAGGA AAGAATGCAA CAGAGGAGGG AGATGTTACG AGATCCAGAA
    ATGAGAAATA AGTTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA TATGGGTCCA
    GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGAACC CTCGGCCTCA GGAAAGTCGG
    ACAGCATTCA GTGGCTCCGT CAGTATTCCG TCTATCACCA AATCCCGCCC TGAACCCGGT
    CGCTCCATGA GCGCCAGCAG TGGCCTATCA GCAAGGTCAT CTGCACAGAA TGGCAGTGCG
    TTAAAGAGAG AATTCTCTGG AGGCAGCTAC AGTGCAAAGC GGCAGCCCAT GCCCTCCCCA
    TCGGAGGGCT CGCTGTCTTC GGGAGGCATG GACCAGGGAA GTGACGCCCC AGCACGGGAC
    TATGACGGAG AGGACTCTGA TTCTCCGCGG CATTCCACGG CTTCTAACAG TTCCAACCTG
    AGCAGTCCTC CCAGCCCAGT GTCGCCCCGG AAGACCAAGA GCCTATCTCT GGAGAGCACA
    GACCGCGGGA GCTGGGACCC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223668
    Clone ID Related Accession (Same CDS sequence) XM_024976361.1
    Accession Version XM_024976361.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase MRCK alpha isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA
    GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT
    GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA
    CATCAACAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA
    GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA
    CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC
    AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC
    AAGCAACAGT ATGAACGAGA AAAAGTGTTA TTAACTGAAG AAAATAAAAA ACTGACAAGT
    GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA
    GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC
    ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA
    GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA
    ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG
    TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT
    AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC
    TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA
    AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC
    GATGCTCTGG ATCAATTTGA ACGCTCTCCA TCCTGTACTC CAGCTAGCAA AGGCAGACGT
    ACTGATCCCG TTGAGAACAC ATATGTACGG AATCCGAACG TCAAGTTTTA CATCCAGTCA
    AGGTCCACCT CTCCTTCCAC ATCTAGTGAA GCTGAGCCAG TTAAGACTGT AGACTCTACT
    CCACTTCCAG TTCATACACC AACCTTAAGG AAAAAGGGAT GCCCTGGTTC AACTGGCTTT
    CCATCGAAGC GCAAGATTCA TCAGTTTTTT GTCAAATCTT TTACTACTCC CACCAAGTGT
    CATCAGTGTA CATCTTTGAT GGTGGGTTTG ATAAGACAAG GCTGTTCTTG TGAAGTGTGT
    GGATTTTCAT GTCACATAAC TTGTGTAAAC AAAGCTCCAA CTGCTTGTCC AGTTCCTCCT
    GAACAGACAA AAGGTCCTCT GGGTATAGAT CCACAGAAAG GAATAGGAAC AGCATATGAA
    GGGCATGTCA GGATCCCTAA GCCAGCTGGA GTGAAGAAAG GATGGCAAAG AGCACTGGCT
    GTAGTCTGTG ACTTCAAGCT CTTTCTGTAC GATATTGCCG AAGGAAAGGC ATCTCAGCCC
    AGTGTTGTAG TCAGTCAAGT GATTGACATG AGGGATGAAG AATTTTCTGT GAGTTCAGTC
    TTGGCTTCTG ATGTTATTCA TGCAAGTCGA AAAGATATAC CCTGTATATT TAGAGTCACA
    GCTTCCCAGC TCTCAGCATC TAGTAACAAA TGTTCCATCC TGATGCTAGC TGATAGTGAA
    AATGAGAAGA GTAAGTGGGT GGGAGTACTG AGTGAATTGC ACAAGATTTT GAAGAAAAAC
    AAATTCAGAG ACCGCTCAGT CTATGTTCCC AAAGAGGCTT ATGACAGCAC TCTGCCCCTC
    ATTAAAACAA CCCAGGCAGC TGCAATCATA GATCATGAAA GAATAGCTTT GGGGAATGAA
    GAAGGGTTAT TTGTTGTGCA TGTCACCAAA GATGAAATTA TTAGAGTTGG TGACAATAAG
    AAGATTCATC AGATAGAACT CATTCCAAAT GATCAACTTG TTGCCGTGAT CTCAGGACGA
    AATCGACACG TCCGACTTTT TCCTATGTCA GCTCTGGACG GGCGAGAGAC TGATTTTTAT
    AAGCTGGCAG AAACTAAAGG CTGTCAAACA ATAACTTCTG GAAAAGTGCG TCATGGAGCT
    CTTACATGCT TGTGTGTGGC TATGAAAAGG CAGGTCCTCT GTTATGAACT ATTTCAGAGC
    AAGACGCGTC ACAGAAAATT TAAGGAAATT CAAGTCCCAT ACAACGTCCA GTGGATGGCA
    ATCTTCAGTG AGCAACTCTG TGTGGGATTC CAGTCTGGAT TTCTAAGATA TCCCCTGAAT
    GGAGAAGGAA GTCCATACAG TATGCTCCAT TCAAATGACA ATACACTCTC ATTTATTGCA
    CATCAACCAA TGGATGCCAT CTGTGCGGTT GAAATCTCCA GTAAAGAATA CCTGCTGTGT
    TTTAACAGCA TTGGAATATA CACTGACTGC CAGGGCCGAA GATCTAGACA ACAGGAACTG
    ATGTGGCCAG CGAATCCTTC CTCTTGTTGT TACAATGCAC CGTATCTCTC AGTGTACAGT
    GAAAATGCAG TTGACATCTT TGATGTGAAC TCCATGGAAT GGATTCAGAC TCTCCCACTC
    AAAAAGGTTC GCCCCTTAAA CAGTGAAGGA TCATTAAATC TTTTAGGCTT GGAGACGATT
    AGATTGATAT ATTTCAAAAA TAAAATGGCA GAAGGGGATG AACTGGTAGT TCCTGAAACA
    TCAGATAATA GTCGGAAGCA AATGGTTAGA AATATTAACA ATAAGAGGCG TTACTCTTTC
    AGAGTTCCGG AAGAGGAAAG AATGCAACAG AGGAGGGAGA TGTTACGAGA TCCAGAAATG
    AGAAATAAGT TAATTTCTAA CCCAACTAAT TTTAACCACA TAGCACATAT GGGTCCAGGA
    GATGGAATAC AGATCCTAAA AGACCTGCCC ATGCCGGGTT TCCCTTATCC ATCCCCTCAT
    CATCACTCCG GTCTGATCTC CTCTCCGATC AACTTTGAGC ACATCTATCA CATGACTGTT
    AATTCTGCTG AAAAATTTCT CTCTCCTGAT TCCATAAACC CTGAATATTC CCCGTCTCTG
    CGCTCTGTCC CCGGTACACC AAGCTTTATG ACTCTGAGGA ACCCTCGGCC TCAGGAAAGT
    CGGACAGCAT TCAGTGGCTC CGTCAGTATT CCGTCTATCA CCAAATCCCG CCCTGAACCC
    GGTCGCTCCA TGAGCGCCAG CAGTGGCCTA TCAGCAAGGT CATCTGCACA GAATGGCAGT
    GCGTTAAAGA GAGAATTCTC TGGAGGCAGC TACAGTGCAA AGCGGCAGCC CATGCCCTCC
    CCATCGGAGG GCTCGCTGTC TTCGGGAGGC ATGGACCAGG GAAGTGACGC CCCAGCACGG
    GACTATGACG GAGAGGACTC TGATTCTCCG CGGCATTCCA CGGCTTCTAA CAGTTCCAAC
    CTGAGCAGTC CTCCCAGCCC AGTGTCGCCC CGGAAGACCA AGAGCCTATC TCTGGAGAGC
    ACAGACCGCG GGAGCTGGGA CCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832129.1
    CDS1282..6528
    Translation

    Target ORF information:

    RefSeq Version XM_024976361.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976361.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA
    GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT
    GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA
    CATCAACAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA
    GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA
    CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC
    AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC
    AAGCAACAGT ATGAACGAGA AAAAGTGTTA TTAACTGAAG AAAATAAAAA ACTGACAAGT
    GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA
    GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC
    ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA
    GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA
    ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG
    TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT
    AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC
    TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA
    AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC
    GATGCTCTGG ATCAATTTGA ACGCTCTCCA TCCTGTACTC CAGCTAGCAA AGGCAGACGT
    ACTGATCCCG TTGAGAACAC ATATGTACGG AATCCGAACG TCAAGTTTTA CATCCAGTCA
    AGGTCCACCT CTCCTTCCAC ATCTAGTGAA GCTGAGCCAG TTAAGACTGT AGACTCTACT
    CCACTTCCAG TTCATACACC AACCTTAAGG AAAAAGGGAT GCCCTGGTTC AACTGGCTTT
    CCATCGAAGC GCAAGATTCA TCAGTTTTTT GTCAAATCTT TTACTACTCC CACCAAGTGT
    CATCAGTGTA CATCTTTGAT GGTGGGTTTG ATAAGACAAG GCTGTTCTTG TGAAGTGTGT
    GGATTTTCAT GTCACATAAC TTGTGTAAAC AAAGCTCCAA CTGCTTGTCC AGTTCCTCCT
    GAACAGACAA AAGGTCCTCT GGGTATAGAT CCACAGAAAG GAATAGGAAC AGCATATGAA
    GGGCATGTCA GGATCCCTAA GCCAGCTGGA GTGAAGAAAG GATGGCAAAG AGCACTGGCT
    GTAGTCTGTG ACTTCAAGCT CTTTCTGTAC GATATTGCCG AAGGAAAGGC ATCTCAGCCC
    AGTGTTGTAG TCAGTCAAGT GATTGACATG AGGGATGAAG AATTTTCTGT GAGTTCAGTC
    TTGGCTTCTG ATGTTATTCA TGCAAGTCGA AAAGATATAC CCTGTATATT TAGAGTCACA
    GCTTCCCAGC TCTCAGCATC TAGTAACAAA TGTTCCATCC TGATGCTAGC TGATAGTGAA
    AATGAGAAGA GTAAGTGGGT GGGAGTACTG AGTGAATTGC ACAAGATTTT GAAGAAAAAC
    AAATTCAGAG ACCGCTCAGT CTATGTTCCC AAAGAGGCTT ATGACAGCAC TCTGCCCCTC
    ATTAAAACAA CCCAGGCAGC TGCAATCATA GATCATGAAA GAATAGCTTT GGGGAATGAA
    GAAGGGTTAT TTGTTGTGCA TGTCACCAAA GATGAAATTA TTAGAGTTGG TGACAATAAG
    AAGATTCATC AGATAGAACT CATTCCAAAT GATCAACTTG TTGCCGTGAT CTCAGGACGA
    AATCGACACG TCCGACTTTT TCCTATGTCA GCTCTGGACG GGCGAGAGAC TGATTTTTAT
    AAGCTGGCAG AAACTAAAGG CTGTCAAACA ATAACTTCTG GAAAAGTGCG TCATGGAGCT
    CTTACATGCT TGTGTGTGGC TATGAAAAGG CAGGTCCTCT GTTATGAACT ATTTCAGAGC
    AAGACGCGTC ACAGAAAATT TAAGGAAATT CAAGTCCCAT ACAACGTCCA GTGGATGGCA
    ATCTTCAGTG AGCAACTCTG TGTGGGATTC CAGTCTGGAT TTCTAAGATA TCCCCTGAAT
    GGAGAAGGAA GTCCATACAG TATGCTCCAT TCAAATGACA ATACACTCTC ATTTATTGCA
    CATCAACCAA TGGATGCCAT CTGTGCGGTT GAAATCTCCA GTAAAGAATA CCTGCTGTGT
    TTTAACAGCA TTGGAATATA CACTGACTGC CAGGGCCGAA GATCTAGACA ACAGGAACTG
    ATGTGGCCAG CGAATCCTTC CTCTTGTTGT TACAATGCAC CGTATCTCTC AGTGTACAGT
    GAAAATGCAG TTGACATCTT TGATGTGAAC TCCATGGAAT GGATTCAGAC TCTCCCACTC
    AAAAAGGTTC GCCCCTTAAA CAGTGAAGGA TCATTAAATC TTTTAGGCTT GGAGACGATT
    AGATTGATAT ATTTCAAAAA TAAAATGGCA GAAGGGGATG AACTGGTAGT TCCTGAAACA
    TCAGATAATA GTCGGAAGCA AATGGTTAGA AATATTAACA ATAAGAGGCG TTACTCTTTC
    AGAGTTCCGG AAGAGGAAAG AATGCAACAG AGGAGGGAGA TGTTACGAGA TCCAGAAATG
    AGAAATAAGT TAATTTCTAA CCCAACTAAT TTTAACCACA TAGCACATAT GGGTCCAGGA
    GATGGAATAC AGATCCTAAA AGACCTGCCC ATGCCGGGTT TCCCTTATCC ATCCCCTCAT
    CATCACTCCG GTCTGATCTC CTCTCCGATC AACTTTGAGC ACATCTATCA CATGACTGTT
    AATTCTGCTG AAAAATTTCT CTCTCCTGAT TCCATAAACC CTGAATATTC CCCGTCTCTG
    CGCTCTGTCC CCGGTACACC AAGCTTTATG ACTCTGAGGA ACCCTCGGCC TCAGGAAAGT
    CGGACAGCAT TCAGTGGCTC CGTCAGTATT CCGTCTATCA CCAAATCCCG CCCTGAACCC
    GGTCGCTCCA TGAGCGCCAG CAGTGGCCTA TCAGCAAGGT CATCTGCACA GAATGGCAGT
    GCGTTAAAGA GAGAATTCTC TGGAGGCAGC TACAGTGCAA AGCGGCAGCC CATGCCCTCC
    CCATCGGAGG GCTCGCTGTC TTCGGGAGGC ATGGACCAGG GAAGTGACGC CCCAGCACGG
    GACTATGACG GAGAGGACTC TGATTCTCCG CGGCATTCCA CGGCTTCTAA CAGTTCCAAC
    CTGAGCAGTC CTCCCAGCCC AGTGTCGCCC CGGAAGACCA AGAGCCTATC TCTGGAGAGC
    ACAGACCGCG GGAGCTGGGA CCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223669
    Clone ID Related Accession (Same CDS sequence) XM_024976362.1
    Accession Version XM_024976362.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase MRCK alpha isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA
    CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC
    CCTGGTTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT
    ACTACTCCCA CCAAGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC
    TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT
    GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA
    ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA
    TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA
    GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA
    TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC
    TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG
    ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAGTGGGTGG GAGTACTGAG TGAATTGCAC
    AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT
    GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA
    ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT
    AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT
    GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG
    CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGA
    AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT
    TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCATAC
    AACGTCCAGT GGATGGCAAT CTTCAGTGAG CAACTCTGTG TGGGATTCCA GTCTGGATTT
    CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT
    ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT
    AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA
    TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG
    TATCTCTCAG TGTACAGTGA AAATGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG
    ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT
    TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA
    CTGGTAGTTC CTGAAACATC AGATAATAGT CGGAAGCAAA TGGTTAGAAA TATTAACAAT
    AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG
    TTACGAGATC CAGAAATGAG AAATAAGTTA ATTTCTAACC CAACTAATTT TAACCACATA
    GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GAACCCTCGG
    CCTCAGGAAA GTCGGACAGC ATTCAGTGGC TCCGTCAGTA TTCCGTCTAT CACCAAATCC
    CGCCCTGAAC CCGGTCGCTC CATGAGCGCC AGCAGTGGCC TATCAGCAAG GTCATCTGCA
    CAGAATGGCA GTGCGTTAAA GAGAGAATTC TCTGGAGGCA GCTACAGTGC AAAGCGGCAG
    CCCATGCCCT CCCCATCGGA GGGCTCGCTG TCTTCGGGAG GCATGGACCA GGGAAGTGAC
    GCCCCAGCAC GGGACTATGA CGGAGAGGAC TCTGATTCTC CGCGGCATTC CACGGCTTCT
    AACAGTTCCA ACCTGAGCAG TCCTCCCAGC CCAGTGTCGC CCCGGAAGAC CAAGAGCCTA
    TCTCTGGAGA GCACAGACCG CGGGAGCTGG GACCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832130.1
    CDS1282..6480
    Translation

    Target ORF information:

    RefSeq Version XM_024976362.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976362.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA
    CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC
    CCTGGTTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT
    ACTACTCCCA CCAAGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC
    TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT
    GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA
    ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA
    TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA
    GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA
    TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC
    TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG
    ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAGTGGGTGG GAGTACTGAG TGAATTGCAC
    AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT
    GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA
    ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT
    AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT
    GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG
    CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGA
    AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT
    TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCATAC
    AACGTCCAGT GGATGGCAAT CTTCAGTGAG CAACTCTGTG TGGGATTCCA GTCTGGATTT
    CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT
    ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT
    AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA
    TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG
    TATCTCTCAG TGTACAGTGA AAATGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG
    ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT
    TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA
    CTGGTAGTTC CTGAAACATC AGATAATAGT CGGAAGCAAA TGGTTAGAAA TATTAACAAT
    AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG
    TTACGAGATC CAGAAATGAG AAATAAGTTA ATTTCTAACC CAACTAATTT TAACCACATA
    GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GAACCCTCGG
    CCTCAGGAAA GTCGGACAGC ATTCAGTGGC TCCGTCAGTA TTCCGTCTAT CACCAAATCC
    CGCCCTGAAC CCGGTCGCTC CATGAGCGCC AGCAGTGGCC TATCAGCAAG GTCATCTGCA
    CAGAATGGCA GTGCGTTAAA GAGAGAATTC TCTGGAGGCA GCTACAGTGC AAAGCGGCAG
    CCCATGCCCT CCCCATCGGA GGGCTCGCTG TCTTCGGGAG GCATGGACCA GGGAAGTGAC
    GCCCCAGCAC GGGACTATGA CGGAGAGGAC TCTGATTCTC CGCGGCATTC CACGGCTTCT
    AACAGTTCCA ACCTGAGCAG TCCTCCCAGC CCAGTGTCGC CCCGGAAGAC CAAGAGCCTA
    TCTCTGGAGA GCACAGACCG CGGGAGCTGG GACCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223670
    Clone ID Related Accession (Same CDS sequence) XM_024976363.1
    Accession Version XM_024976363.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase MRCK alpha isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA
    GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT
    GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA
    CATCAACAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA
    GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA
    CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC
    AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC
    AAGCAACAGT ATGAACGAGA AAAAGTGTTA TTAACTGAAG AAAATAAAAA ACTGACAAGT
    GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA
    GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC
    ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA
    GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA
    ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG
    TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT
    AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC
    TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA
    AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC
    GATGCTCTGG ATCAATTTGA AACTGTAGAC TCTACTCCAC TTCCAGTTCA TACACCAACC
    TTAAGGAAAA AGGGATGCCC TGGTTCAACT GGCTTTCCAT CGAAGCGCAA GATTCATCAG
    TTTTTTGTCA AATCTTTTAC TACTCCCACC AAGTGTCATC AGTGTACATC TTTGATGGTG
    GGTTTGATAA GACAAGGCTG TTCTTGTGAA GTGTGTGGAT TTTCATGTCA CATAACTTGT
    GTAAACAAAG CTCCAACTGC TTGTCCAGTT CCTCCTGAAC AGACAAAAGG TCCTCTGGGT
    ATAGATCCAC AGAAAGGAAT AGGAACAGCA TATGAAGGGC ATGTCAGGAT CCCTAAGCCA
    GCTGGAGTGA AGAAAGGATG GCAAAGAGCA CTGGCTGTAG TCTGTGACTT CAAGCTCTTT
    CTGTACGATA TTGCCGAAGG AAAGGCATCT CAGCCCAGTG TTGTAGTCAG TCAAGTGATT
    GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTCTTGG CTTCTGATGT TATTCATGCA
    AGTCGAAAAG ATATACCCTG TATATTTAGA GTCACAGCTT CCCAGCTCTC AGCATCTAGT
    AACAAATGTT CCATCCTGAT GCTAGCTGAT AGTGAAAATG AGAAGAGTAA GTGGGTGGGA
    GTACTGAGTG AATTGCACAA GATTTTGAAG AAAAACAAAT TCAGAGACCG CTCAGTCTAT
    GTTCCCAAAG AGGCTTATGA CAGCACTCTG CCCCTCATTA AAACAACCCA GGCAGCTGCA
    ATCATAGATC ATGAAAGAAT AGCTTTGGGG AATGAAGAAG GGTTATTTGT TGTGCATGTC
    ACCAAAGATG AAATTATTAG AGTTGGTGAC AATAAGAAGA TTCATCAGAT AGAACTCATT
    CCAAATGATC AACTTGTTGC CGTGATCTCA GGACGAAATC GACACGTCCG ACTTTTTCCT
    ATGTCAGCTC TGGACGGGCG AGAGACTGAT TTTTATAAGC TGGCAGAAAC TAAAGGCTGT
    CAAACAATAA CTTCTGGAAA AGTGCGTCAT GGAGCTCTTA CATGCTTGTG TGTGGCTATG
    AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CGCGTCACAG AAAATTTAAG
    GAAATTCAAG TCCCATACAA CGTCCAGTGG ATGGCAATCT TCAGTGAGCA ACTCTGTGTG
    GGATTCCAGT CTGGATTTCT AAGATATCCC CTGAATGGAG AAGGAAGTCC ATACAGTATG
    CTCCATTCAA ATGACAATAC ACTCTCATTT ATTGCACATC AACCAATGGA TGCCATCTGT
    GCGGTTGAAA TCTCCAGTAA AGAATACCTG CTGTGTTTTA ACAGCATTGG AATATACACT
    GACTGCCAGG GCCGAAGATC TAGACAACAG GAACTGATGT GGCCAGCGAA TCCTTCCTCT
    TGTTGTTACA ATGCACCGTA TCTCTCAGTG TACAGTGAAA ATGCAGTTGA CATCTTTGAT
    GTGAACTCCA TGGAATGGAT TCAGACTCTC CCACTCAAAA AGGTTCGCCC CTTAAACAGT
    GAAGGATCAT TAAATCTTTT AGGCTTGGAG ACGATTAGAT TGATATATTT CAAAAATAAA
    ATGGCAGAAG GGGATGAACT GGTAGTTCCT GAAACATCAG ATAATAGTCG GAAGCAAATG
    GTTAGAAATA TTAACAATAA GAGGCGTTAC TCTTTCAGAG TTCCGGAAGA GGAAAGAATG
    CAACAGAGGA GGGAGATGTT ACGAGATCCA GAAATGAGAA ATAAGTTAAT TTCTAACCCA
    ACTAATTTTA ACCACATAGC ACATATGGGT CCAGGAGATG GAATACAGAT CCTAAAAGAC
    CTGCCCATGA ACCCTCGGCC TCAGGAAAGT CGGACAGCAT TCAGTGGCTC CGTCAGTATT
    CCGTCTATCA CCAAATCCCG CCCTGAACCC GGTCGCTCCA TGAGCGCCAG CAGTGGCCTA
    TCAGCAAGGT CATCTGCACA GAATGGCAGT GCGTTAAAGA GAGAATTCTC TGGAGGCAGC
    TACAGTGCAA AGCGGCAGCC CATGCCCTCC CCATCGGAGG GCTCGCTGTC TTCGGGAGGC
    ATGGACCAGG GAAGTGACGC CCCAGCACGG GACTATGACG GAGAGGACTC TGATTCTCCG
    CGGCATTCCA CGGCTTCTAA CAGTTCCAAC CTGAGCAGTC CTCCCAGCCC AGTGTCGCCC
    CGGAAGACCA AGAGCCTATC TCTGGAGAGC ACAGACCGCG GGAGCTGGGA CCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832131.1
    CDS1282..6198
    Translation

    Target ORF information:

    RefSeq Version XM_024976363.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976363.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA
    GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT
    GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA
    CATCAACAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA
    GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA
    CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC
    AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC
    AAGCAACAGT ATGAACGAGA AAAAGTGTTA TTAACTGAAG AAAATAAAAA ACTGACAAGT
    GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA
    GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC
    ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA
    GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA
    ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG
    TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT
    AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC
    TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA
    AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC
    GATGCTCTGG ATCAATTTGA AACTGTAGAC TCTACTCCAC TTCCAGTTCA TACACCAACC
    TTAAGGAAAA AGGGATGCCC TGGTTCAACT GGCTTTCCAT CGAAGCGCAA GATTCATCAG
    TTTTTTGTCA AATCTTTTAC TACTCCCACC AAGTGTCATC AGTGTACATC TTTGATGGTG
    GGTTTGATAA GACAAGGCTG TTCTTGTGAA GTGTGTGGAT TTTCATGTCA CATAACTTGT
    GTAAACAAAG CTCCAACTGC TTGTCCAGTT CCTCCTGAAC AGACAAAAGG TCCTCTGGGT
    ATAGATCCAC AGAAAGGAAT AGGAACAGCA TATGAAGGGC ATGTCAGGAT CCCTAAGCCA
    GCTGGAGTGA AGAAAGGATG GCAAAGAGCA CTGGCTGTAG TCTGTGACTT CAAGCTCTTT
    CTGTACGATA TTGCCGAAGG AAAGGCATCT CAGCCCAGTG TTGTAGTCAG TCAAGTGATT
    GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTCTTGG CTTCTGATGT TATTCATGCA
    AGTCGAAAAG ATATACCCTG TATATTTAGA GTCACAGCTT CCCAGCTCTC AGCATCTAGT
    AACAAATGTT CCATCCTGAT GCTAGCTGAT AGTGAAAATG AGAAGAGTAA GTGGGTGGGA
    GTACTGAGTG AATTGCACAA GATTTTGAAG AAAAACAAAT TCAGAGACCG CTCAGTCTAT
    GTTCCCAAAG AGGCTTATGA CAGCACTCTG CCCCTCATTA AAACAACCCA GGCAGCTGCA
    ATCATAGATC ATGAAAGAAT AGCTTTGGGG AATGAAGAAG GGTTATTTGT TGTGCATGTC
    ACCAAAGATG AAATTATTAG AGTTGGTGAC AATAAGAAGA TTCATCAGAT AGAACTCATT
    CCAAATGATC AACTTGTTGC CGTGATCTCA GGACGAAATC GACACGTCCG ACTTTTTCCT
    ATGTCAGCTC TGGACGGGCG AGAGACTGAT TTTTATAAGC TGGCAGAAAC TAAAGGCTGT
    CAAACAATAA CTTCTGGAAA AGTGCGTCAT GGAGCTCTTA CATGCTTGTG TGTGGCTATG
    AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CGCGTCACAG AAAATTTAAG
    GAAATTCAAG TCCCATACAA CGTCCAGTGG ATGGCAATCT TCAGTGAGCA ACTCTGTGTG
    GGATTCCAGT CTGGATTTCT AAGATATCCC CTGAATGGAG AAGGAAGTCC ATACAGTATG
    CTCCATTCAA ATGACAATAC ACTCTCATTT ATTGCACATC AACCAATGGA TGCCATCTGT
    GCGGTTGAAA TCTCCAGTAA AGAATACCTG CTGTGTTTTA ACAGCATTGG AATATACACT
    GACTGCCAGG GCCGAAGATC TAGACAACAG GAACTGATGT GGCCAGCGAA TCCTTCCTCT
    TGTTGTTACA ATGCACCGTA TCTCTCAGTG TACAGTGAAA ATGCAGTTGA CATCTTTGAT
    GTGAACTCCA TGGAATGGAT TCAGACTCTC CCACTCAAAA AGGTTCGCCC CTTAAACAGT
    GAAGGATCAT TAAATCTTTT AGGCTTGGAG ACGATTAGAT TGATATATTT CAAAAATAAA
    ATGGCAGAAG GGGATGAACT GGTAGTTCCT GAAACATCAG ATAATAGTCG GAAGCAAATG
    GTTAGAAATA TTAACAATAA GAGGCGTTAC TCTTTCAGAG TTCCGGAAGA GGAAAGAATG
    CAACAGAGGA GGGAGATGTT ACGAGATCCA GAAATGAGAA ATAAGTTAAT TTCTAACCCA
    ACTAATTTTA ACCACATAGC ACATATGGGT CCAGGAGATG GAATACAGAT CCTAAAAGAC
    CTGCCCATGA ACCCTCGGCC TCAGGAAAGT CGGACAGCAT TCAGTGGCTC CGTCAGTATT
    CCGTCTATCA CCAAATCCCG CCCTGAACCC GGTCGCTCCA TGAGCGCCAG CAGTGGCCTA
    TCAGCAAGGT CATCTGCACA GAATGGCAGT GCGTTAAAGA GAGAATTCTC TGGAGGCAGC
    TACAGTGCAA AGCGGCAGCC CATGCCCTCC CCATCGGAGG GCTCGCTGTC TTCGGGAGGC
    ATGGACCAGG GAAGTGACGC CCCAGCACGG GACTATGACG GAGAGGACTC TGATTCTCCG
    CGGCATTCCA CGGCTTCTAA CAGTTCCAAC CTGAGCAGTC CTCCCAGCCC AGTGTCGCCC
    CGGAAGACCA AGAGCCTATC TCTGGAGAGC ACAGACCGCG GGAGCTGGGA CCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37050
    Clone ID Related Accession (Same CDS sequence) NM_001192937.1
    Accession Version NM_001192937.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5160bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase MRCK alpha
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03087251.1, AAFC03087250.1, AAFC03044739.1, AAFC03017398.1, AAFC03044738.1, AAFC03017401.1, AAFC02012075.1, AAFC03093465.1, AAFC03093464.1 and AAFC03093466.1. On Jul 17, 2010 this sequence version replaced XM_618596.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG AGTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA
    ACCTTAAGGA AAAAGGGATG CCCTGGTTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT
    CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAAGTGTC ATCAGTGTAC ATCTTTGATG
    GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT
    TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG
    GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG
    CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC
    TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG
    ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT
    GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT
    AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAGTGGGTG
    GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC
    TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT
    GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT
    GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC
    ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT
    CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC
    TGTCAAACAA TAACTTCTGG AAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT
    ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT
    AAGGAAATTC AAGTCCCATA CAACGTCCAG TGGATGGCAA TCTTCAGTGA GCAACTCTGT
    GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT
    ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC
    TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC
    ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC
    TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAATGCAGT TGACATCTTT
    GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC
    AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT
    AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CAGATAATAG TCGGAAGCAA
    ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA
    ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAGTT AATTTCTAAC
    CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA
    GACCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCCGTCAGT
    ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC
    CTATCAGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC
    AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCTTCGGGA
    GGCATGGACC AGGGAAGTGA CGCCCCAGCA CGGGACTATG ACGGAGAGGA CTCTGATTCT
    CCGCGGCATT CCACGGCTTC TAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCAGTGTCG
    CCCCGGAAGA CCAAGAGCCT ATCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179866.1
    CDS263..5422
    Translation

    Target ORF information:

    RefSeq Version NM_001192937.1
    Organism Bos taurus(cattle)
    Definition Bos taurus CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192937.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
    ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT
    GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT
    AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA
    AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT
    AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA
    TGTTTTCGTG AAGAAAGGGA TGTGTTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG
    CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG
    GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTGC CTGAAGACAT GGCTCGATTT
    TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA
    GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT
    GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT
    CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA
    CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA
    CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG
    TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA
    CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC
    CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA
    GAAGTTAGTA GCCCAACAGA CACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT
    TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT
    GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTACG AGTTACAGCT
    GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA
    ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA
    AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA
    CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG
    AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG
    CAAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA
    CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC
    CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG
    GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC
    CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA
    CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT
    GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG
    AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA
    GAACATCAAC AAGAAATAAC CAAACTAAAG AGTGATTTGG AAAAGAAAAG TATCTTTTAT
    GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA
    GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA
    GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG
    TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTATTAACTG AAGAAAATAA AAAACTGACA
    AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA
    GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG
    ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC
    TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA
    GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG
    GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG
    AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG
    AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT
    GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT
    ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA
    ACCTTAAGGA AAAAGGGATG CCCTGGTTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT
    CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAAGTGTC ATCAGTGTAC ATCTTTGATG
    GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT
    TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG
    GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG
    CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC
    TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG
    ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT
    GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT
    AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAGTGGGTG
    GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC
    TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT
    GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT
    GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC
    ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT
    CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC
    TGTCAAACAA TAACTTCTGG AAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT
    ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT
    AAGGAAATTC AAGTCCCATA CAACGTCCAG TGGATGGCAA TCTTCAGTGA GCAACTCTGT
    GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT
    ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC
    TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC
    ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC
    TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAATGCAGT TGACATCTTT
    GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC
    AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT
    AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CAGATAATAG TCGGAAGCAA
    ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA
    ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAGTT AATTTCTAAC
    CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA
    GACCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCCGTCAGT
    ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC
    CTATCAGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC
    AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCTTCGGGA
    GGCATGGACC AGGGAAGTGA CGCCCCAGCA CGGGACTATG ACGGAGAGGA CTCTGATTCT
    CCGCGGCATT CCACGGCTTC TAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCAGTGTCG
    CCCCGGAAGA CCAAGAGCCT ATCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.