TRIO cDNA ORF clone, Bos taurus(cattle)

The following TRIO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa228050 XM_024981342.1
Latest version!
Bos taurus trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa228051 XM_024981343.1
Latest version!
Bos taurus trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa37677 NM_001102212.1
Latest version!
Bos taurus trio Rho guanine nucleotide exchange factor (TRIO), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa228048 XM_024981340.1
Latest version!
Bos taurus trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa228049 XM_024981341.1
Latest version!
Bos taurus trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa228050
    Clone ID Related Accession (Same CDS sequence) XM_024981342.1
    Accession Version XM_024981342.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product triple functional domain protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGACTCTTA ATCGTGATGG CAAGTCTGAC GGAAGGTGTA ATTCTCAAAA AGTGCTGGAC 
    TGGATCGAGA ACCATGGAGA AGCATTTCTG AGCAAACACA CAGGTGTAGG GAAATCGCTC
    CATCGGGCCA GGGCTTTGCA GAAACGTCAT GAGGATTTTG AAGAAGTAGC ACAGAACACG
    TACACCAATG CAGATAAGTT ACTTGAGGCA GCCGAGCAGC TGGCCCAGAC GGGAGAATGT
    GACCCGGAAG AGATCTATCA GGCTGCCCAT CAGCTCGAGG ACCGGATACA GGATTTTGTG
    CGGCGCGTGG AGCAGCGGAA GATCCTCCTG GACATGTCGG TGTCTTTTCA CACGCATGTG
    AAAGAGCTGT GGACATGGCT GGAGGAGCTG CAGAAGGAGC TGCTGGACGA CGTGTACGCC
    GAGTCCGTGG AGGCCGTGCA GGACCTCAGC AAGCGCTTCG GCCAGCAGCA GCAGACCACC
    CTGCAGGTGA CTGTGAACGT CATCAAGGAA GGCGAGGACC TGATCCAGCA GCTGAGGGAC
    TCAGCCATCT CCAGCAACAA GACGCCGCAC AACAGCTCCA TCAGCCACAT CGAAACGGTG
    CTGCAGCAGC TGGACGAGGC CCAGGCGCAG ATGGAGGAGC TCTTCCAGGA GCGCAAGATC
    AAGCTGGAGC TCTTCCTGCA GCTGCGCATC TTCGAGCGGG ACGCCATCGA TATCATCTCA
    GACCTGGAGT CTTGGAACGA TGAGCTTTCT CAGCAGATGA ACGACTTCGA CACGGAGGAC
    CTCACGATCG CGGAGCAGCG CCTGCAGCAC CACGCGGACA AGGCCCTGAC CATGAACAAC
    CTGACCTTCG ACGTCATCCA CCAGGGGCAG GACCTGCTGC AGTACGTCAA CGAGGTGCAG
    GCCTCCGGTG TGGAGCTGCT TTGCGACAGA GATGTGGACA TGGCCACGCG GGTCCAGGAC
    CTCCTGGAGT TTCTGCACGA GAAGCAGCAG GAACTGGACC TCGCAGCTGA GCAGCACCGG
    AAGCACCTGG AGCAGTGCGT GCAGCTGCGC CACCTGCAGG CCGAGGTCAA GCAGGTGCTG
    GGCTGGATCC GCAATGGAGA GTCCATGCTG AACGCCGGGC TCATCGCGGC CAGCTCGCTG
    CAGGAGGCAG AGCAGCTGCA GAGGGAGCAC GAGCAGTTCC AGCACGCCAT CGAGAAGACA
    CACCAGAGCG CCCTGCAGGT CCAGCAGAAG GCCGAGGCCA TGCTGCAGGC CAATCACTAC
    GACATGGACA TGATCCGGGA CTGTGCCGAG AAGGTCGCCT CACACTGGCA GCAGCTCATG
    CTCAAGATGG AAGACCGGCT GAAGCTGGTG AACGCGTCCG TGGCCTTCTA CAAGACCTCA
    GAGCAGGTCT GCAGCGTCCT GGAGAGCCTG GAGCAGGAGT ACAAGAGGGA AGAGGACTGG
    TGCGGAGGGG CCGACAAGCT GGGCCCCAAC TCCGAGACGG ACCACGTCAC CCCCATGATC
    AGCAAGCACC TCGAACAGAA GGAAGCATTC CTGAAGGCTT GCACGCTTGC CAGGAGGAAC
    GCGGACGTCT TCCTGAAGTA CCTGCACAGG AACAGCGTGA ACATGCCAGG CATGGTGACG
    CACATCAAGG CCCCCGAGCA GCAAGTGAAA AATATCTTGA ACGAGCTCTT CCAGCGGGAG
    AACAGGGTGC TGCACTACTG GACCATGAGG AAGCGGCGGC TGGACCAGTG CCAGCAGTTC
    GTGGTCTTCG AGCGAAGCGC CAAGCAGGCT CTGGAGTGGA TCCACGACAG CGGGGAGTTC
    TACCTCTCCA CCCACACGTC CACGGGCTCA AGCATCCAGC ACACCCAGGA GCTGCTGAAG
    GAGCATGAGG ACTTCCAGAT CACCGCCAAG CAAACCAAAG AGAGAGTGAA GCTATTGATA
    CAGCTGGCCG ACGGCTTTTG TGAAAAGGGG CATGCCCATG CGGCAGAAAT AAAAAAATGT
    GTCACTGCCG TGGATAAGAG GTACAGAGAC TTCTCTCTGC GGATGGAGAA ATACAGGACC
    TCTTTGGAGA AAGCCCTAGG GATTTCTTCA GATTCCAACA AATCGAGCAA AAGTCTTCAG
    CTGGATATTG TTCCGGCCAG TATCCCCGGC TCAGAGGTGA AACTCCGAGA CGCCGCCCAT
    GAACTTAATG AGGAAAAGCG GAAATCTGCC CGCAGGAAAG AGTTCATAAT GGCTGAGCTA
    ATTCAAACTG AAAAGGCTTA CGTAAGAGAC CTCCGGGAGT GTATGGATAC CTACCTGTGG
    GAAATGACCA GCGGCGTGGA GGAGATCCCC CCGGGCATTG TAAACAAAGA GCTCATCATC
    TTCGGGAACA TGCAGGAAAT CTACGAGTTT CACAATAACA TATTCCTAAA AGAGCTGGAA
    AAATATGAGC AGTTGCCGGA GGACGTGGGA CATTGCTTTG TAACCTGGGC AGACAAGTTT
    CAGATGTATG TCACATATTG CAAAAATAAG CCTGATTCTA CTCAGCTGAT ATTAGAGCAT
    GCAGGATCCT ACTTTGATGA AATACAGCAG CGGCACGGAT TAGCCAATTC CATCTCCTCC
    TACCTTATTA AACCAGTTCA GCGAATAACG AAATATCAGC TCCTTTTAAA AGAGCTGCTC
    ACGTGCTGCG AGGAGGGGAA GGGGGAGATC AAGGATGGGC TGGAGGTGAT GCTCAGCGTG
    CCTAAGCGGG CCAACGATGC CATGCACCTC AGCATGCTGG AAGGGTTCGA TGAGAACATT
    GAGTCTCAGG GAGAGCTGAT CCTGCAGGAA TCCTTCCAAG TGTGGGACCC CAAAACCTTA
    ATCCGAAAGG GCCGTGAGCG GCACCTTTTC CTGTTTGAGA TGTCCTTGGT GTTTAGTAAA
    GAAGTAAAAG ATTCCAGTGG CAGAAGCAAG TACCTTTATA AAAGCAAATT GTTTACCTCA
    GAGCTGGGTG TCACAGAGCA CGTTGAAGGA GACCCTTGCA AATTCGCACT GTGGGTGGGG
    AGGACACCAA CTTCAGATAA TAAGATTGTC CTTAAGGCCT CCAGTATTGA GAACAAGCAG
    GACTGGATCA AGCATATCCG GGAGGTGATC CAGGAAAGGA CCATCCACCT GAAAGGCGCC
    CTGAAGGAGC CCATCCACAT CCCCAAAACA GCTCCAGCCA CGAGACAGAA GGGAAGGAGG
    GATGGAGAGG ATTTGGACAG CCAAGGAGAT GGCAGCAGCC AGCCCGATAC GATTTCCATT
    GCCTCCCGGA CGTCTCAGAA CACGCTGGAC AGTGACAAGC TGTCCGGCGG CTGCGAGCTG
    ACCGTGGTGA TCCACGACTT CACGGCCTGC AACAGCACCG AGCTCACCAT CCGCCGCGGC
    CAGACCGTGG AAGTCTTGGA GCGGCCCCAT GACAAACCCG ACTGGTGCCT GGTGCGGACC
    ACTGACCGGT CGCCTGCGGC CGAGGGCCTG GTCCCCTGTG GCTCCCTGTG CATCGCCCAC
    TCCAGGAGCA GCATGGAGAT GGAGGGCATC TTCAACCACA AAGACTCACT GTCCGTCTCC
    AGCAACGACG CCAGCCCGCC CGCCTCCGTG GCCTCCCTCC AGCCCCACAT GGTAGGGGCG
    CAGAGCTCGC CGGGCCCCAA GCGCCCGGGC AACACGCTGC GTAAGTGGCT GACAAGCCCC
    GTGCGGCGGC TGAGCAGCGG CAAGGCGGAT GGGCACGTGA AGAAGCTGGC GCACAAGCAC
    AAGAAGAGCC GCGAGGTCCG CAAGAGCGCC GACGCCGGCT CGCAGAAGGA CTCGGACGAC
    AGCGCGGCCA CCCCGCAGGA CGAGACCGTG GAAGAGAGAG GCCGGAACGA GGGCCTGAGC
    AGTGGCACGC TTTCCAAGTC CTCATCTTCG GGCATGCAGA GCTGCGGAGA GGAAGAAGGG
    GAGGAGGGGG CCGACGCCGT GCCCCTGCCG CCACCCATGG CCATCCAGCA GCACAGCCTG
    CTCCAGCCAG ACTCCCAGGA CGACAAGGCC TCTTCTCGGT TATTAGTCCG CCCCACCAGC
    TCCGAAACAC CGAGCGCAGC CGAGCTTGTC AGTGCAATCG AGGAACTCGT GAAAAGCAAG
    ATGGCTCTGG AGGATCGTCC CAGCTCGCTC CTCGTCGACC AGGGGGACAG CAGCAGCCCC
    TCCTTCAACC CTTCAGACAA CTCCCTGCTG TCCTCCTCCT CACCCATCGA TGAGATGGAA
    GAGAGGAAGT CCAGCTCTTT AAAGAGACGC CACTACGTTT TACAAGAATT AGTGGAGACA
    GAGCGTGACT ATGTGCGGGA CCTCGGCTGT GTGGTGGAGG GCTACATGGC ACTTATGAAA
    GAAGATGGTG TTCCTGACGA CATGAAAGGC AAAGACAAGA TTGTGTTTGG CAACATCCAT
    CAGATTTACG ACTGGCACAG AGACTTTTTT TTAGGAGAGT TGGAGAAGTG CCTTGAAGAT
    CCGGAAAAAC TAGGATCCCT TTTTGTTAAA CATGAGAGAA GGTTGCACAT GTACATAGTT
    TATTGCCAAA ATAAACCAAA GTCTGAACAC ATTGTCTCAG AATACATTGA CACCTTTTTT
    GAGGACTTAA AGCAGCGCCT GGGCCACAGG CTGCAGCTCA CGGACTTGCT GATCAAACCC
    GTTCAGAGGA TCACGAAGTA CCAGCTGCTG CTGAAGGACT TCCTCAAGTA TTCCAAAAAG
    GCCAGCCTGG ATACATCAGA ACTAGAGAGA GCCGTGGAGG TCATGTGCGT CGTCCCGAAG
    CGCTGCAACG ACATGATGAA CGTCGGGAGG CTACAAGGCT TCGACGGTAA GATCGTGGCC
    CAGGGCAAGC TGCTCCTGCA GGACACGTTC TTGGTCACGG ACCAGGACAC GGGGCTGCTG
    CCTCGCTGCA AAGAGAGGCG GGTGTTCCTC TTCGAGCAGA TCGTCATTTT CAGTGAGCCG
    CTGGATAAAA AAAAGGGCTT CTCCATGCCA GGATTCCTGT TCAAGAACAG TATCAAGGTG
    AGCTGCCTTT GCCTGGAGGA AAACGTGGAA AATGATCCCT GTAAGTTCGC TCTGACATCG
    CGGACGGGTG ACGTGGTGGA GACTTTCATC CTGCACTCAT CCAGTCCAAG TGTCCGGCAG
    ACGTGGATCC ATGAAATCAA CCAGATTTTA GAAAACCAGC GCAATTTTTT AAACGCCTTG
    ACATCACCCA TCGAATATCA GAGGAACCAC AGCGGGGGCG GCGGCAGCGG CGGGGGCGGG
    GGCAGCAGCG GGGCCCCCAG CGGCGGGGGC GGCGGCAGCA ACCACAGCGG CGGTGGGGGC
    GGCCCCGGTG GCGGCGGCAG CGGCAGCTCG AGCAGAAGTA GACCCTCCCG GATTCCCCAG
    CCCGTCAGAC ACCACTCCCC CGTGCTGGTC TCCTCTGCAG CCTCGAGCCA GGCAGAGGCA
    GACAAGATGT CAGGTACGTG CACCCCGGGC CCGCCACCGC CTCCTCCCAG CCGCAGCCCC
    GCCCCGGAGG CCAGCGCCGG CCCGCCTGGC AGGGCGCCCC CCAGCAGCGA GCCCGACGGT
    CCCGAGCGAG ACACCGAGCC GATCCCCAAG ATGAAGGTGC TGGAGAGCCC CCGGAAGTCG
    TCCGGGGGCG TGCTGCCACA GGACGGAGCC GCCAAGGAGG CACCGCGGAG CGGCCCGGGC
    CCCCTTCCGC TGGGCAAGGC CAGGCCCGGG GCCCCCTCGC CCCTGCACTC ACCTCTGGCC
    GCCGCCGCCG CGTCCCCCTC TCCGTTCGGC AAGGAGCCCT TGCCCCCCAG CAGCCCCCTG
    CAGAAGGGGG GCTCCTTCTG GAGCTCCGTG CCTGCCTCCC CTGCCAGCCG GCCCGGCTCC
    TTCACCTTCC CGGGGGACAG CGACTCCCTG CAGCGGCAGG CACGGCGCCA CGCGGCCCCT
    GGCAAGGATG CGGACCGCAT GAGCACGTGC TCCTCGGCCA GCGAGCAGTC CGTGCAGTCC
    ACCCAGAGCA ACGGGAGTGA AAGCAGCAGC GGTAGCAACA TCTCCACCAT GTTGGTGACA
    CACGACTACA CGGCAGTGAA GGAGGATGAG ATAAACGTCT ATCAAGGAGA GGTCGTTCAA
    ATTCTGGCCA GCAATCAACA GAACATGTTT CTGGTGTTCC GAGCCGCCAC TGACCAGTGC
    CCCGCAGCCG AGGGCTGGAT TCCGGGCTTC GTCCTGGGGC ACACCAGCGC AGTCATCATG
    GAGAACCCTG ACGGGACTCT CAAGAAGTCA ACATCTTGGC ACACAGCACT CCGCTTAAGG
    AAAAAATCTG AGAAAAAAGA TAAAGACGGC AAACGGGAAG GCAAGTTAGA GAACGGTTAC
    CGGAAGTCTC GGGAAGGACT GAGCAACAAG GTATCTGTGA AGCTTCTCAA TCCCAACTAC
    ATATATGACG TTCCCCCAGA ATTCGTCATT CCCTTGAGTG AGGTCACGTG TGAGGCAGGC
    GAGACCGTTG TTCTTCGATG TCGGGTTTGT GGCCGCCCCA AGGCCTCCAT CACCTGGAAG
    GGCCCTGAAC ACAACACCTT GAACAACGAC GGTCACTACA GCATCTCCTA CAGCGACTTG
    GGAGAGGCCG CTCTGAAGAT CGTGGGTGTG GCCGCCGAGG ACGACGGCGT CTACACGTGC
    ATCGCGGTGA ACGACATGGG GTCAGCCTCG TCTTCAGCCA GCCTGAGGGT TCTAGGTCCA
    GGGAGTGATG GCATCGTGGT GACCTGGAAG GACAACTTTG ACTGCTTCTA CAGCGAGGTG
    GCCGAGCTCG GCAGGGGCAG ATTCTCTGTC GTGAAGAAAT GTGATCAGAA AGGCACCAAA
    CGAGCCGTGG CCACTAAGTT TGTGAACAAG AAGCTGATGA AGCGTGACCA GGTCACCCAC
    GAACTTGGGA TCCTGCAGAA CCTCCAGCAC CCGCTGCTGG TGGGCCTCCT GGACACCTTC
    GAGACCCCCA CCAGCTACGT GCTGGTCCTG GAGATGGCCG ACCAGGGACG GCTCCTGGAC
    TGCGTGGTGC GGTGGGGGAA CCTCACGGAG GGGAAGATCC GGGCGTACCT GGGGGAGGTT
    CTGGAAGCCG TGCGATACCT TCACAACTGC AGGATAGTGC ATCTGGACCT AAAGCCTGAG
    AACATCCTCG TGGATCAGAG CTCAGCCAAG CCGACCATCA AGCTGGCTGA CTTCGGAGAT
    GCCGTCCAAC TCAACACGAC CTACCACATC CACCCATTGC TGGGGAACCC CGAGTTCGCA
    GCCCCCGAGA TCATCCTGGG GAACCCGGTC TCCCTGACCT CAGACACGTG GAGCGTGGGC
    GTGCTGGCCT ACGTGCTTCT CAGCGGCGTG TCCCCCTTCC TGGACGACAG CGTGGAAGAG
    ACCTGCCTCA ACATTTGCCG CCTGGACTTC AGCTTCCCAG AGGACTACTT CCAAGGAGTG
    AGCCAGAGGG CCAAGGACTT CGTGTGCTTC CTACTGCACG AGGACCCCGC CAAGCGCCCC
    TCGGCTGCGT CGGCCCTCCA GGAGCGGTGG CTGCAGGCGG GCCACGGCCA CGGCAAAGGC
    GCGGGCGTCC TCGACACGTC CAGACTGACC TCCTTCATTG AGCGGCGCAA ACACCAGAAC
    GACGTTCGAC CTATCCGTAG CATGAAAAAC TTCTTACAGA GCAGGCTTCT GCCTCGAGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837110.1
    CDS235..7857
    Translation

    Target ORF information:

    RefSeq Version XM_024981342.1
    Organism Bos taurus(cattle)
    Definition Bos taurus trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981342.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGACTCTTA ATCGTGATGG CAAGTCTGAC GGAAGGTGTA ATTCTCAAAA AGTGCTGGAC 
    TGGATCGAGA ACCATGGAGA AGCATTTCTG AGCAAACACA CAGGTGTAGG GAAATCGCTC
    CATCGGGCCA GGGCTTTGCA GAAACGTCAT GAGGATTTTG AAGAAGTAGC ACAGAACACG
    TACACCAATG CAGATAAGTT ACTTGAGGCA GCCGAGCAGC TGGCCCAGAC GGGAGAATGT
    GACCCGGAAG AGATCTATCA GGCTGCCCAT CAGCTCGAGG ACCGGATACA GGATTTTGTG
    CGGCGCGTGG AGCAGCGGAA GATCCTCCTG GACATGTCGG TGTCTTTTCA CACGCATGTG
    AAAGAGCTGT GGACATGGCT GGAGGAGCTG CAGAAGGAGC TGCTGGACGA CGTGTACGCC
    GAGTCCGTGG AGGCCGTGCA GGACCTCAGC AAGCGCTTCG GCCAGCAGCA GCAGACCACC
    CTGCAGGTGA CTGTGAACGT CATCAAGGAA GGCGAGGACC TGATCCAGCA GCTGAGGGAC
    TCAGCCATCT CCAGCAACAA GACGCCGCAC AACAGCTCCA TCAGCCACAT CGAAACGGTG
    CTGCAGCAGC TGGACGAGGC CCAGGCGCAG ATGGAGGAGC TCTTCCAGGA GCGCAAGATC
    AAGCTGGAGC TCTTCCTGCA GCTGCGCATC TTCGAGCGGG ACGCCATCGA TATCATCTCA
    GACCTGGAGT CTTGGAACGA TGAGCTTTCT CAGCAGATGA ACGACTTCGA CACGGAGGAC
    CTCACGATCG CGGAGCAGCG CCTGCAGCAC CACGCGGACA AGGCCCTGAC CATGAACAAC
    CTGACCTTCG ACGTCATCCA CCAGGGGCAG GACCTGCTGC AGTACGTCAA CGAGGTGCAG
    GCCTCCGGTG TGGAGCTGCT TTGCGACAGA GATGTGGACA TGGCCACGCG GGTCCAGGAC
    CTCCTGGAGT TTCTGCACGA GAAGCAGCAG GAACTGGACC TCGCAGCTGA GCAGCACCGG
    AAGCACCTGG AGCAGTGCGT GCAGCTGCGC CACCTGCAGG CCGAGGTCAA GCAGGTGCTG
    GGCTGGATCC GCAATGGAGA GTCCATGCTG AACGCCGGGC TCATCGCGGC CAGCTCGCTG
    CAGGAGGCAG AGCAGCTGCA GAGGGAGCAC GAGCAGTTCC AGCACGCCAT CGAGAAGACA
    CACCAGAGCG CCCTGCAGGT CCAGCAGAAG GCCGAGGCCA TGCTGCAGGC CAATCACTAC
    GACATGGACA TGATCCGGGA CTGTGCCGAG AAGGTCGCCT CACACTGGCA GCAGCTCATG
    CTCAAGATGG AAGACCGGCT GAAGCTGGTG AACGCGTCCG TGGCCTTCTA CAAGACCTCA
    GAGCAGGTCT GCAGCGTCCT GGAGAGCCTG GAGCAGGAGT ACAAGAGGGA AGAGGACTGG
    TGCGGAGGGG CCGACAAGCT GGGCCCCAAC TCCGAGACGG ACCACGTCAC CCCCATGATC
    AGCAAGCACC TCGAACAGAA GGAAGCATTC CTGAAGGCTT GCACGCTTGC CAGGAGGAAC
    GCGGACGTCT TCCTGAAGTA CCTGCACAGG AACAGCGTGA ACATGCCAGG CATGGTGACG
    CACATCAAGG CCCCCGAGCA GCAAGTGAAA AATATCTTGA ACGAGCTCTT CCAGCGGGAG
    AACAGGGTGC TGCACTACTG GACCATGAGG AAGCGGCGGC TGGACCAGTG CCAGCAGTTC
    GTGGTCTTCG AGCGAAGCGC CAAGCAGGCT CTGGAGTGGA TCCACGACAG CGGGGAGTTC
    TACCTCTCCA CCCACACGTC CACGGGCTCA AGCATCCAGC ACACCCAGGA GCTGCTGAAG
    GAGCATGAGG ACTTCCAGAT CACCGCCAAG CAAACCAAAG AGAGAGTGAA GCTATTGATA
    CAGCTGGCCG ACGGCTTTTG TGAAAAGGGG CATGCCCATG CGGCAGAAAT AAAAAAATGT
    GTCACTGCCG TGGATAAGAG GTACAGAGAC TTCTCTCTGC GGATGGAGAA ATACAGGACC
    TCTTTGGAGA AAGCCCTAGG GATTTCTTCA GATTCCAACA AATCGAGCAA AAGTCTTCAG
    CTGGATATTG TTCCGGCCAG TATCCCCGGC TCAGAGGTGA AACTCCGAGA CGCCGCCCAT
    GAACTTAATG AGGAAAAGCG GAAATCTGCC CGCAGGAAAG AGTTCATAAT GGCTGAGCTA
    ATTCAAACTG AAAAGGCTTA CGTAAGAGAC CTCCGGGAGT GTATGGATAC CTACCTGTGG
    GAAATGACCA GCGGCGTGGA GGAGATCCCC CCGGGCATTG TAAACAAAGA GCTCATCATC
    TTCGGGAACA TGCAGGAAAT CTACGAGTTT CACAATAACA TATTCCTAAA AGAGCTGGAA
    AAATATGAGC AGTTGCCGGA GGACGTGGGA CATTGCTTTG TAACCTGGGC AGACAAGTTT
    CAGATGTATG TCACATATTG CAAAAATAAG CCTGATTCTA CTCAGCTGAT ATTAGAGCAT
    GCAGGATCCT ACTTTGATGA AATACAGCAG CGGCACGGAT TAGCCAATTC CATCTCCTCC
    TACCTTATTA AACCAGTTCA GCGAATAACG AAATATCAGC TCCTTTTAAA AGAGCTGCTC
    ACGTGCTGCG AGGAGGGGAA GGGGGAGATC AAGGATGGGC TGGAGGTGAT GCTCAGCGTG
    CCTAAGCGGG CCAACGATGC CATGCACCTC AGCATGCTGG AAGGGTTCGA TGAGAACATT
    GAGTCTCAGG GAGAGCTGAT CCTGCAGGAA TCCTTCCAAG TGTGGGACCC CAAAACCTTA
    ATCCGAAAGG GCCGTGAGCG GCACCTTTTC CTGTTTGAGA TGTCCTTGGT GTTTAGTAAA
    GAAGTAAAAG ATTCCAGTGG CAGAAGCAAG TACCTTTATA AAAGCAAATT GTTTACCTCA
    GAGCTGGGTG TCACAGAGCA CGTTGAAGGA GACCCTTGCA AATTCGCACT GTGGGTGGGG
    AGGACACCAA CTTCAGATAA TAAGATTGTC CTTAAGGCCT CCAGTATTGA GAACAAGCAG
    GACTGGATCA AGCATATCCG GGAGGTGATC CAGGAAAGGA CCATCCACCT GAAAGGCGCC
    CTGAAGGAGC CCATCCACAT CCCCAAAACA GCTCCAGCCA CGAGACAGAA GGGAAGGAGG
    GATGGAGAGG ATTTGGACAG CCAAGGAGAT GGCAGCAGCC AGCCCGATAC GATTTCCATT
    GCCTCCCGGA CGTCTCAGAA CACGCTGGAC AGTGACAAGC TGTCCGGCGG CTGCGAGCTG
    ACCGTGGTGA TCCACGACTT CACGGCCTGC AACAGCACCG AGCTCACCAT CCGCCGCGGC
    CAGACCGTGG AAGTCTTGGA GCGGCCCCAT GACAAACCCG ACTGGTGCCT GGTGCGGACC
    ACTGACCGGT CGCCTGCGGC CGAGGGCCTG GTCCCCTGTG GCTCCCTGTG CATCGCCCAC
    TCCAGGAGCA GCATGGAGAT GGAGGGCATC TTCAACCACA AAGACTCACT GTCCGTCTCC
    AGCAACGACG CCAGCCCGCC CGCCTCCGTG GCCTCCCTCC AGCCCCACAT GGTAGGGGCG
    CAGAGCTCGC CGGGCCCCAA GCGCCCGGGC AACACGCTGC GTAAGTGGCT GACAAGCCCC
    GTGCGGCGGC TGAGCAGCGG CAAGGCGGAT GGGCACGTGA AGAAGCTGGC GCACAAGCAC
    AAGAAGAGCC GCGAGGTCCG CAAGAGCGCC GACGCCGGCT CGCAGAAGGA CTCGGACGAC
    AGCGCGGCCA CCCCGCAGGA CGAGACCGTG GAAGAGAGAG GCCGGAACGA GGGCCTGAGC
    AGTGGCACGC TTTCCAAGTC CTCATCTTCG GGCATGCAGA GCTGCGGAGA GGAAGAAGGG
    GAGGAGGGGG CCGACGCCGT GCCCCTGCCG CCACCCATGG CCATCCAGCA GCACAGCCTG
    CTCCAGCCAG ACTCCCAGGA CGACAAGGCC TCTTCTCGGT TATTAGTCCG CCCCACCAGC
    TCCGAAACAC CGAGCGCAGC CGAGCTTGTC AGTGCAATCG AGGAACTCGT GAAAAGCAAG
    ATGGCTCTGG AGGATCGTCC CAGCTCGCTC CTCGTCGACC AGGGGGACAG CAGCAGCCCC
    TCCTTCAACC CTTCAGACAA CTCCCTGCTG TCCTCCTCCT CACCCATCGA TGAGATGGAA
    GAGAGGAAGT CCAGCTCTTT AAAGAGACGC CACTACGTTT TACAAGAATT AGTGGAGACA
    GAGCGTGACT ATGTGCGGGA CCTCGGCTGT GTGGTGGAGG GCTACATGGC ACTTATGAAA
    GAAGATGGTG TTCCTGACGA CATGAAAGGC AAAGACAAGA TTGTGTTTGG CAACATCCAT
    CAGATTTACG ACTGGCACAG AGACTTTTTT TTAGGAGAGT TGGAGAAGTG CCTTGAAGAT
    CCGGAAAAAC TAGGATCCCT TTTTGTTAAA CATGAGAGAA GGTTGCACAT GTACATAGTT
    TATTGCCAAA ATAAACCAAA GTCTGAACAC ATTGTCTCAG AATACATTGA CACCTTTTTT
    GAGGACTTAA AGCAGCGCCT GGGCCACAGG CTGCAGCTCA CGGACTTGCT GATCAAACCC
    GTTCAGAGGA TCACGAAGTA CCAGCTGCTG CTGAAGGACT TCCTCAAGTA TTCCAAAAAG
    GCCAGCCTGG ATACATCAGA ACTAGAGAGA GCCGTGGAGG TCATGTGCGT CGTCCCGAAG
    CGCTGCAACG ACATGATGAA CGTCGGGAGG CTACAAGGCT TCGACGGTAA GATCGTGGCC
    CAGGGCAAGC TGCTCCTGCA GGACACGTTC TTGGTCACGG ACCAGGACAC GGGGCTGCTG
    CCTCGCTGCA AAGAGAGGCG GGTGTTCCTC TTCGAGCAGA TCGTCATTTT CAGTGAGCCG
    CTGGATAAAA AAAAGGGCTT CTCCATGCCA GGATTCCTGT TCAAGAACAG TATCAAGGTG
    AGCTGCCTTT GCCTGGAGGA AAACGTGGAA AATGATCCCT GTAAGTTCGC TCTGACATCG
    CGGACGGGTG ACGTGGTGGA GACTTTCATC CTGCACTCAT CCAGTCCAAG TGTCCGGCAG
    ACGTGGATCC ATGAAATCAA CCAGATTTTA GAAAACCAGC GCAATTTTTT AAACGCCTTG
    ACATCACCCA TCGAATATCA GAGGAACCAC AGCGGGGGCG GCGGCAGCGG CGGGGGCGGG
    GGCAGCAGCG GGGCCCCCAG CGGCGGGGGC GGCGGCAGCA ACCACAGCGG CGGTGGGGGC
    GGCCCCGGTG GCGGCGGCAG CGGCAGCTCG AGCAGAAGTA GACCCTCCCG GATTCCCCAG
    CCCGTCAGAC ACCACTCCCC CGTGCTGGTC TCCTCTGCAG CCTCGAGCCA GGCAGAGGCA
    GACAAGATGT CAGGTACGTG CACCCCGGGC CCGCCACCGC CTCCTCCCAG CCGCAGCCCC
    GCCCCGGAGG CCAGCGCCGG CCCGCCTGGC AGGGCGCCCC CCAGCAGCGA GCCCGACGGT
    CCCGAGCGAG ACACCGAGCC GATCCCCAAG ATGAAGGTGC TGGAGAGCCC CCGGAAGTCG
    TCCGGGGGCG TGCTGCCACA GGACGGAGCC GCCAAGGAGG CACCGCGGAG CGGCCCGGGC
    CCCCTTCCGC TGGGCAAGGC CAGGCCCGGG GCCCCCTCGC CCCTGCACTC ACCTCTGGCC
    GCCGCCGCCG CGTCCCCCTC TCCGTTCGGC AAGGAGCCCT TGCCCCCCAG CAGCCCCCTG
    CAGAAGGGGG GCTCCTTCTG GAGCTCCGTG CCTGCCTCCC CTGCCAGCCG GCCCGGCTCC
    TTCACCTTCC CGGGGGACAG CGACTCCCTG CAGCGGCAGG CACGGCGCCA CGCGGCCCCT
    GGCAAGGATG CGGACCGCAT GAGCACGTGC TCCTCGGCCA GCGAGCAGTC CGTGCAGTCC
    ACCCAGAGCA ACGGGAGTGA AAGCAGCAGC GGTAGCAACA TCTCCACCAT GTTGGTGACA
    CACGACTACA CGGCAGTGAA GGAGGATGAG ATAAACGTCT ATCAAGGAGA GGTCGTTCAA
    ATTCTGGCCA GCAATCAACA GAACATGTTT CTGGTGTTCC GAGCCGCCAC TGACCAGTGC
    CCCGCAGCCG AGGGCTGGAT TCCGGGCTTC GTCCTGGGGC ACACCAGCGC AGTCATCATG
    GAGAACCCTG ACGGGACTCT CAAGAAGTCA ACATCTTGGC ACACAGCACT CCGCTTAAGG
    AAAAAATCTG AGAAAAAAGA TAAAGACGGC AAACGGGAAG GCAAGTTAGA GAACGGTTAC
    CGGAAGTCTC GGGAAGGACT GAGCAACAAG GTATCTGTGA AGCTTCTCAA TCCCAACTAC
    ATATATGACG TTCCCCCAGA ATTCGTCATT CCCTTGAGTG AGGTCACGTG TGAGGCAGGC
    GAGACCGTTG TTCTTCGATG TCGGGTTTGT GGCCGCCCCA AGGCCTCCAT CACCTGGAAG
    GGCCCTGAAC ACAACACCTT GAACAACGAC GGTCACTACA GCATCTCCTA CAGCGACTTG
    GGAGAGGCCG CTCTGAAGAT CGTGGGTGTG GCCGCCGAGG ACGACGGCGT CTACACGTGC
    ATCGCGGTGA ACGACATGGG GTCAGCCTCG TCTTCAGCCA GCCTGAGGGT TCTAGGTCCA
    GGGAGTGATG GCATCGTGGT GACCTGGAAG GACAACTTTG ACTGCTTCTA CAGCGAGGTG
    GCCGAGCTCG GCAGGGGCAG ATTCTCTGTC GTGAAGAAAT GTGATCAGAA AGGCACCAAA
    CGAGCCGTGG CCACTAAGTT TGTGAACAAG AAGCTGATGA AGCGTGACCA GGTCACCCAC
    GAACTTGGGA TCCTGCAGAA CCTCCAGCAC CCGCTGCTGG TGGGCCTCCT GGACACCTTC
    GAGACCCCCA CCAGCTACGT GCTGGTCCTG GAGATGGCCG ACCAGGGACG GCTCCTGGAC
    TGCGTGGTGC GGTGGGGGAA CCTCACGGAG GGGAAGATCC GGGCGTACCT GGGGGAGGTT
    CTGGAAGCCG TGCGATACCT TCACAACTGC AGGATAGTGC ATCTGGACCT AAAGCCTGAG
    AACATCCTCG TGGATCAGAG CTCAGCCAAG CCGACCATCA AGCTGGCTGA CTTCGGAGAT
    GCCGTCCAAC TCAACACGAC CTACCACATC CACCCATTGC TGGGGAACCC CGAGTTCGCA
    GCCCCCGAGA TCATCCTGGG GAACCCGGTC TCCCTGACCT CAGACACGTG GAGCGTGGGC
    GTGCTGGCCT ACGTGCTTCT CAGCGGCGTG TCCCCCTTCC TGGACGACAG CGTGGAAGAG
    ACCTGCCTCA ACATTTGCCG CCTGGACTTC AGCTTCCCAG AGGACTACTT CCAAGGAGTG
    AGCCAGAGGG CCAAGGACTT CGTGTGCTTC CTACTGCACG AGGACCCCGC CAAGCGCCCC
    TCGGCTGCGT CGGCCCTCCA GGAGCGGTGG CTGCAGGCGG GCCACGGCCA CGGCAAAGGC
    GCGGGCGTCC TCGACACGTC CAGACTGACC TCCTTCATTG AGCGGCGCAA ACACCAGAAC
    GACGTTCGAC CTATCCGTAG CATGAAAAAC TTCTTACAGA GCAGGCTTCT GCCTCGAGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228051
    Clone ID Related Accession (Same CDS sequence) XM_024981343.1
    Accession Version XM_024981343.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product triple functional domain protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGAACAAGT GCCTTCAGGT GCTGGACTGG ATCGAGAACC ATGGAGAAGC ATTTCTGAGC 
    AAACACACAG GTGTAGGGAA ATCGCTCCAT CGGGCCAGGG CTTTGCAGAA ACGTCATGAG
    GATTTTGAAG AAGTAGCACA GAACACGTAC ACCAATGCAG ATAAGTTACT TGAGGCAGCC
    GAGCAGCTGG CCCAGACGGG AGAATGTGAC CCGGAAGAGA TCTATCAGGC TGCCCATCAG
    CTCGAGGACC GGATACAGGA TTTTGTGCGG CGCGTGGAGC AGCGGAAGAT CCTCCTGGAC
    ATGTCGGTGT CTTTTCACAC GCATGTGAAA GAGCTGTGGA CATGGCTGGA GGAGCTGCAG
    AAGGAGCTGC TGGACGACGT GTACGCCGAG TCCGTGGAGG CCGTGCAGGA CCTCAGCAAG
    CGCTTCGGCC AGCAGCAGCA GACCACCCTG CAGGTGACTG TGAACGTCAT CAAGGAAGGC
    GAGGACCTGA TCCAGCAGCT GAGGGACTCA GCCATCTCCA GCAACAAGAC GCCGCACAAC
    AGCTCCATCA GCCACATCGA AACGGTGCTG CAGCAGCTGG ACGAGGCCCA GGCGCAGATG
    GAGGAGCTCT TCCAGGAGCG CAAGATCAAG CTGGAGCTCT TCCTGCAGCT GCGCATCTTC
    GAGCGGGACG CCATCGATAT CATCTCAGAC CTGGAGTCTT GGAACGATGA GCTTTCTCAG
    CAGATGAACG ACTTCGACAC GGAGGACCTC ACGATCGCGG AGCAGCGCCT GCAGCACCAC
    GCGGACAAGG CCCTGACCAT GAACAACCTG ACCTTCGACG TCATCCACCA GGGGCAGGAC
    CTGCTGCAGT ACGTCAACGA GGTGCAGGCC TCCGGTGTGG AGCTGCTTTG CGACAGAGAT
    GTGGACATGG CCACGCGGGT CCAGGACCTC CTGGAGTTTC TGCACGAGAA GCAGCAGGAA
    CTGGACCTCG CAGCTGAGCA GCACCGGAAG CACCTGGAGC AGTGCGTGCA GCTGCGCCAC
    CTGCAGGCCG AGGTCAAGCA GGTGCTGGGC TGGATCCGCA ATGGAGAGTC CATGCTGAAC
    GCCGGGCTCA TCGCGGCCAG CTCGCTGCAG GAGGCAGAGC AGCTGCAGAG GGAGCACGAG
    CAGTTCCAGC ACGCCATCGA GAAGACACAC CAGAGCGCCC TGCAGGTCCA GCAGAAGGCC
    GAGGCCATGC TGCAGGCCAA TCACTACGAC ATGGACATGA TCCGGGACTG TGCCGAGAAG
    GTCGCCTCAC ACTGGCAGCA GCTCATGCTC AAGATGGAAG ACCGGCTGAA GCTGGTGAAC
    GCGTCCGTGG CCTTCTACAA GACCTCAGAG CAGGTCTGCA GCGTCCTGGA GAGCCTGGAG
    CAGGAGTACA AGAGGGAAGA GGACTGGTGC GGAGGGGCCG ACAAGCTGGG CCCCAACTCC
    GAGACGGACC ACGTCACCCC CATGATCAGC AAGCACCTCG AACAGAAGGA AGCATTCCTG
    AAGGCTTGCA CGCTTGCCAG GAGGAACGCG GACGTCTTCC TGAAGTACCT GCACAGGAAC
    AGCGTGAACA TGCCAGGCAT GGTGACGCAC ATCAAGGCCC CCGAGCAGCA AGTGAAAAAT
    ATCTTGAACG AGCTCTTCCA GCGGGAGAAC AGGGTGCTGC ACTACTGGAC CATGAGGAAG
    CGGCGGCTGG ACCAGTGCCA GCAGTTCGTG GTCTTCGAGC GAAGCGCCAA GCAGGCTCTG
    GAGTGGATCC ACGACAGCGG GGAGTTCTAC CTCTCCACCC ACACGTCCAC GGGCTCAAGC
    ATCCAGCACA CCCAGGAGCT GCTGAAGGAG CATGAGGACT TCCAGATCAC CGCCAAGCAA
    ACCAAAGAGA GAGTGAAGCT ATTGATACAG CTGGCCGACG GCTTTTGTGA AAAGGGGCAT
    GCCCATGCGG CAGAAATAAA AAAATGTGTC ACTGCCGTGG ATAAGAGGTA CAGAGACTTC
    TCTCTGCGGA TGGAGAAATA CAGGACCTCT TTGGAGAAAG CCCTAGGGAT TTCTTCAGAT
    TCCAACAAAT CGAGCAAAAG TCTTCAGCTG GATATTGTTC CGGCCAGTAT CCCCGGCTCA
    GAGGTGAAAC TCCGAGACGC CGCCCATGAA CTTAATGAGG AAAAGCGGAA ATCTGCCCGC
    AGGAAAGAGT TCATAATGGC TGAGCTAATT CAAACTGAAA AGGCTTACGT AAGAGACCTC
    CGGGAGTGTA TGGATACCTA CCTGTGGGAA ATGACCAGCG GCGTGGAGGA GATCCCCCCG
    GGCATTGTAA ACAAAGAGCT CATCATCTTC GGGAACATGC AGGAAATCTA CGAGTTTCAC
    AATAACATAT TCCTAAAAGA GCTGGAAAAA TATGAGCAGT TGCCGGAGGA CGTGGGACAT
    TGCTTTGTAA CCTGGGCAGA CAAGTTTCAG ATGTATGTCA CATATTGCAA AAATAAGCCT
    GATTCTACTC AGCTGATATT AGAGCATGCA GGATCCTACT TTGATGAAAT ACAGCAGCGG
    CACGGATTAG CCAATTCCAT CTCCTCCTAC CTTATTAAAC CAGTTCAGCG AATAACGAAA
    TATCAGCTCC TTTTAAAAGA GCTGCTCACG TGCTGCGAGG AGGGGAAGGG GGAGATCAAG
    GATGGGCTGG AGGTGATGCT CAGCGTGCCT AAGCGGGCCA ACGATGCCAT GCACCTCAGC
    ATGCTGGAAG GGTTCGATGA GAACATTGAG TCTCAGGGAG AGCTGATCCT GCAGGAATCC
    TTCCAAGTGT GGGACCCCAA AACCTTAATC CGAAAGGGCC GTGAGCGGCA CCTTTTCCTG
    TTTGAGATGT CCTTGGTGTT TAGTAAAGAA GTAAAAGATT CCAGTGGCAG AAGCAAGTAC
    CTTTATAAAA GCAAATTGTT TACCTCAGAG CTGGGTGTCA CAGAGCACGT TGAAGGAGAC
    CCTTGCAAAT TCGCACTGTG GGTGGGGAGG ACACCAACTT CAGATAATAA GATTGTCCTT
    AAGGCCTCCA GTATTGAGAA CAAGCAGGAC TGGATCAAGC ATATCCGGGA GGTGATCCAG
    GAAAGGACCA TCCACCTGAA AGGCGCCCTG AAGGAGCCCA TCCACATCCC CAAAACAGCT
    CCAGCCACGA GACAGAAGGG AAGGAGGGAT GGAGAGGATT TGGACAGCCA AGGAGATGGC
    AGCAGCCAGC CCGATACGAT TTCCATTGCC TCCCGGACGT CTCAGAACAC GCTGGACAGT
    GACAAGCTGT CCGGCGGCTG CGAGCTGACC GTGGTGATCC ACGACTTCAC GGCCTGCAAC
    AGCACCGAGC TCACCATCCG CCGCGGCCAG ACCGTGGAAG TCTTGGAGCG GCCCCATGAC
    AAACCCGACT GGTGCCTGGT GCGGACCACT GACCGGTCGC CTGCGGCCGA GGGCCTGGTC
    CCCTGTGGCT CCCTGTGCAT CGCCCACTCC AGGAGCAGCA TGGAGATGGA GGGCATCTTC
    AACCACAAAG ACTCACTGTC CGTCTCCAGC AACGACGCCA GCCCGCCCGC CTCCGTGGCC
    TCCCTCCAGC CCCACATGGT AGGGGCGCAG AGCTCGCCGG GCCCCAAGCG CCCGGGCAAC
    ACGCTGCGTA AGTGGCTGAC AAGCCCCGTG CGGCGGCTGA GCAGCGGCAA GGCGGATGGG
    CACGTGAAGA AGCTGGCGCA CAAGCACAAG AAGAGCCGCG AGGTCCGCAA GAGCGCCGAC
    GCCGGCTCGC AGAAGGACTC GGACGACAGC GCGGCCACCC CGCAGGACGA GACCGTGGAA
    GAGAGAGGCC GGAACGAGGG CCTGAGCAGT GGCACGCTTT CCAAGTCCTC ATCTTCGGGC
    ATGCAGAGCT GCGGAGAGGA AGAAGGGGAG GAGGGGGCCG ACGCCGTGCC CCTGCCGCCA
    CCCATGGCCA TCCAGCAGCA CAGCCTGCTC CAGCCAGACT CCCAGGACGA CAAGGCCTCT
    TCTCGGTTAT TAGTCCGCCC CACCAGCTCC GAAACACCGA GCGCAGCCGA GCTTGTCAGT
    GCAATCGAGG AACTCGTGAA AAGCAAGATG GCTCTGGAGG ATCGTCCCAG CTCGCTCCTC
    GTCGACCAGG GGGACAGCAG CAGCCCCTCC TTCAACCCTT CAGACAACTC CCTGCTGTCC
    TCCTCCTCAC CCATCGATGA GATGGAAGAG AGGAAGTCCA GCTCTTTAAA GAGACGCCAC
    TACGTTTTAC AAGAATTAGT GGAGACAGAG CGTGACTATG TGCGGGACCT CGGCTGTGTG
    GTGGAGGGCT ACATGGCACT TATGAAAGAA GATGGTGTTC CTGACGACAT GAAAGGCAAA
    GACAAGATTG TGTTTGGCAA CATCCATCAG ATTTACGACT GGCACAGAGA CTTTTTTTTA
    GGAGAGTTGG AGAAGTGCCT TGAAGATCCG GAAAAACTAG GATCCCTTTT TGTTAAACAT
    GAGAGAAGGT TGCACATGTA CATAGTTTAT TGCCAAAATA AACCAAAGTC TGAACACATT
    GTCTCAGAAT ACATTGACAC CTTTTTTGAG GACTTAAAGC AGCGCCTGGG CCACAGGCTG
    CAGCTCACGG ACTTGCTGAT CAAACCCGTT CAGAGGATCA CGAAGTACCA GCTGCTGCTG
    AAGGACTTCC TCAAGTATTC CAAAAAGGCC AGCCTGGATA CATCAGAACT AGAGAGAGCC
    GTGGAGGTCA TGTGCGTCGT CCCGAAGCGC TGCAACGACA TGATGAACGT CGGGAGGCTA
    CAAGGCTTCG ACGGTAAGAT CGTGGCCCAG GGCAAGCTGC TCCTGCAGGA CACGTTCTTG
    GTCACGGACC AGGACACGGG GCTGCTGCCT CGCTGCAAAG AGAGGCGGGT GTTCCTCTTC
    GAGCAGATCG TCATTTTCAG TGAGCCGCTG GATAAAAAAA AGGGCTTCTC CATGCCAGGA
    TTCCTGTTCA AGAACAGTAT CAAGGTGAGC TGCCTTTGCC TGGAGGAAAA CGTGGAAAAT
    GATCCCTGTA AGTTCGCTCT GACATCGCGG ACGGGTGACG TGGTGGAGAC TTTCATCCTG
    CACTCATCCA GTCCAAGTGT CCGGCAGACG TGGATCCATG AAATCAACCA GATTTTAGAA
    AACCAGCGCA ATTTTTTAAA CGCCTTGACA TCACCCATCG AATATCAGAG GAACCACAGC
    GGGGGCGGCG GCAGCGGCGG GGGCGGGGGC AGCAGCGGGG CCCCCAGCGG CGGGGGCGGC
    GGCAGCAACC ACAGCGGCGG TGGGGGCGGC CCCGGTGGCG GCGGCAGCGG CAGCTCGAGC
    AGAAGTAGAC CCTCCCGGAT TCCCCAGCCC GTCAGACACC ACTCCCCCGT GCTGGTCTCC
    TCTGCAGCCT CGAGCCAGGC AGAGGCAGAC AAGATGTCAG GTACGTGCAC CCCGGGCCCG
    CCACCGCCTC CTCCCAGCCG CAGCCCCGCC CCGGAGGCCA GCGCCGGCCC GCCTGGCAGG
    GCGCCCCCCA GCAGCGAGCC CGACGGTCCC GAGCGAGACA CCGAGCCGAT CCCCAAGATG
    AAGGTGCTGG AGAGCCCCCG GAAGTCGTCC GGGGGCGTGC TGCCACAGGA CGGAGCCGCC
    AAGGAGGCAC CGCGGAGCGG CCCGGGCCCC CTTCCGCTGG GCAAGGCCAG GCCCGGGGCC
    CCCTCGCCCC TGCACTCACC TCTGGCCGCC GCCGCCGCGT CCCCCTCTCC GTTCGGCAAG
    GAGCCCTTGC CCCCCAGCAG CCCCCTGCAG AAGGGGGGCT CCTTCTGGAG CTCCGTGCCT
    GCCTCCCCTG CCAGCCGGCC CGGCTCCTTC ACCTTCCCGG GGGACAGCGA CTCCCTGCAG
    CGGCAGGCAC GGCGCCACGC GGCCCCTGGC AAGGATGCGG ACCGCATGAG CACGTGCTCC
    TCGGCCAGCG AGCAGTCCGT GCAGTCCACC CAGAGCAACG GGAGTGAAAG CAGCAGCGGT
    AGCAACATCT CCACCATGTT GGTGACACAC GACTACACGG CAGTGAAGGA GGATGAGATA
    AACGTCTATC AAGGAGAGGT CGTTCAAATT CTGGCCAGCA ATCAACAGAA CATGTTTCTG
    GTGTTCCGAG CCGCCACTGA CCAGTGCCCC GCAGCCGAGG GCTGGATTCC GGGCTTCGTC
    CTGGGGCACA CCAGCGCAGT CATCATGGAG AACCCTGACG GGACTCTCAA GAAGTCAACA
    TCTTGGCACA CAGCACTCCG CTTAAGGAAA AAATCTGAGA AAAAAGATAA AGACGGCAAA
    CGGGAAGGCA AGTTAGAGAA CGGTTACCGG AAGTCTCGGG AAGGACTGAG CAACAAGGTA
    TCTGTGAAGC TTCTCAATCC CAACTACATA TATGACGTTC CCCCAGAATT CGTCATTCCC
    TTGAGTGAGG TCACGTGTGA GGCAGGCGAG ACCGTTGTTC TTCGATGTCG GGTTTGTGGC
    CGCCCCAAGG CCTCCATCAC CTGGAAGGGC CCTGAACACA ACACCTTGAA CAACGACGGT
    CACTACAGCA TCTCCTACAG CGACTTGGGA GAGGCCGCTC TGAAGATCGT GGGTGTGGCC
    GCCGAGGACG ACGGCGTCTA CACGTGCATC GCGGTGAACG ACATGGGGTC AGCCTCGTCT
    TCAGCCAGCC TGAGGGTTCT AGGTCCAGGG AGTGATGGCA TCGTGGTGAC CTGGAAGGAC
    AACTTTGACT GCTTCTACAG CGAGGTGGCC GAGCTCGGCA GGGGCAGATT CTCTGTCGTG
    AAGAAATGTG ATCAGAAAGG CACCAAACGA GCCGTGGCCA CTAAGTTTGT GAACAAGAAG
    CTGATGAAGC GTGACCAGGT CACCCACGAA CTTGGGATCC TGCAGAACCT CCAGCACCCG
    CTGCTGGTGG GCCTCCTGGA CACCTTCGAG ACCCCCACCA GCTACGTGCT GGTCCTGGAG
    ATGGCCGACC AGGGACGGCT CCTGGACTGC GTGGTGCGGT GGGGGAACCT CACGGAGGGG
    AAGATCCGGG CGTACCTGGG GGAGGTTCTG GAAGCCGTGC GATACCTTCA CAACTGCAGG
    ATAGTGCATC TGGACCTAAA GCCTGAGAAC ATCCTCGTGG ATCAGAGCTC AGCCAAGCCG
    ACCATCAAGC TGGCTGACTT CGGAGATGCC GTCCAACTCA ACACGACCTA CCACATCCAC
    CCATTGCTGG GGAACCCCGA GTTCGCAGCC CCCGAGATCA TCCTGGGGAA CCCGGTCTCC
    CTGACCTCAG ACACGTGGAG CGTGGGCGTG CTGGCCTACG TGCTTCTCAG CGGCGTGTCC
    CCCTTCCTGG ACGACAGCGT GGAAGAGACC TGCCTCAACA TTTGCCGCCT GGACTTCAGC
    TTCCCAGAGG ACTACTTCCA AGGAGTGAGC CAGAGGGCCA AGGACTTCGT GTGCTTCCTA
    CTGCACGAGG ACCCCGCCAA GCGCCCCTCG GCTGCGTCGG CCCTCCAGGA GCGGTGGCTG
    CAGGCGGGCC ACGGCCACGG CAAAGGCGCG GGCGTCCTCG ACACGTCCAG ACTGACCTCC
    TTCATTGAGC GGCGCAAACA CCAGAACGAC GTTCGACCTA TCCGTAGCAT GAAAAACTTC
    TTACAGAGCA GGCTTCTGCC TCGAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837111.1
    CDS16..7605
    Translation

    Target ORF information:

    RefSeq Version XM_024981343.1
    Organism Bos taurus(cattle)
    Definition Bos taurus trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981343.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGAACAAGT GCCTTCAGGT GCTGGACTGG ATCGAGAACC ATGGAGAAGC ATTTCTGAGC 
    AAACACACAG GTGTAGGGAA ATCGCTCCAT CGGGCCAGGG CTTTGCAGAA ACGTCATGAG
    GATTTTGAAG AAGTAGCACA GAACACGTAC ACCAATGCAG ATAAGTTACT TGAGGCAGCC
    GAGCAGCTGG CCCAGACGGG AGAATGTGAC CCGGAAGAGA TCTATCAGGC TGCCCATCAG
    CTCGAGGACC GGATACAGGA TTTTGTGCGG CGCGTGGAGC AGCGGAAGAT CCTCCTGGAC
    ATGTCGGTGT CTTTTCACAC GCATGTGAAA GAGCTGTGGA CATGGCTGGA GGAGCTGCAG
    AAGGAGCTGC TGGACGACGT GTACGCCGAG TCCGTGGAGG CCGTGCAGGA CCTCAGCAAG
    CGCTTCGGCC AGCAGCAGCA GACCACCCTG CAGGTGACTG TGAACGTCAT CAAGGAAGGC
    GAGGACCTGA TCCAGCAGCT GAGGGACTCA GCCATCTCCA GCAACAAGAC GCCGCACAAC
    AGCTCCATCA GCCACATCGA AACGGTGCTG CAGCAGCTGG ACGAGGCCCA GGCGCAGATG
    GAGGAGCTCT TCCAGGAGCG CAAGATCAAG CTGGAGCTCT TCCTGCAGCT GCGCATCTTC
    GAGCGGGACG CCATCGATAT CATCTCAGAC CTGGAGTCTT GGAACGATGA GCTTTCTCAG
    CAGATGAACG ACTTCGACAC GGAGGACCTC ACGATCGCGG AGCAGCGCCT GCAGCACCAC
    GCGGACAAGG CCCTGACCAT GAACAACCTG ACCTTCGACG TCATCCACCA GGGGCAGGAC
    CTGCTGCAGT ACGTCAACGA GGTGCAGGCC TCCGGTGTGG AGCTGCTTTG CGACAGAGAT
    GTGGACATGG CCACGCGGGT CCAGGACCTC CTGGAGTTTC TGCACGAGAA GCAGCAGGAA
    CTGGACCTCG CAGCTGAGCA GCACCGGAAG CACCTGGAGC AGTGCGTGCA GCTGCGCCAC
    CTGCAGGCCG AGGTCAAGCA GGTGCTGGGC TGGATCCGCA ATGGAGAGTC CATGCTGAAC
    GCCGGGCTCA TCGCGGCCAG CTCGCTGCAG GAGGCAGAGC AGCTGCAGAG GGAGCACGAG
    CAGTTCCAGC ACGCCATCGA GAAGACACAC CAGAGCGCCC TGCAGGTCCA GCAGAAGGCC
    GAGGCCATGC TGCAGGCCAA TCACTACGAC ATGGACATGA TCCGGGACTG TGCCGAGAAG
    GTCGCCTCAC ACTGGCAGCA GCTCATGCTC AAGATGGAAG ACCGGCTGAA GCTGGTGAAC
    GCGTCCGTGG CCTTCTACAA GACCTCAGAG CAGGTCTGCA GCGTCCTGGA GAGCCTGGAG
    CAGGAGTACA AGAGGGAAGA GGACTGGTGC GGAGGGGCCG ACAAGCTGGG CCCCAACTCC
    GAGACGGACC ACGTCACCCC CATGATCAGC AAGCACCTCG AACAGAAGGA AGCATTCCTG
    AAGGCTTGCA CGCTTGCCAG GAGGAACGCG GACGTCTTCC TGAAGTACCT GCACAGGAAC
    AGCGTGAACA TGCCAGGCAT GGTGACGCAC ATCAAGGCCC CCGAGCAGCA AGTGAAAAAT
    ATCTTGAACG AGCTCTTCCA GCGGGAGAAC AGGGTGCTGC ACTACTGGAC CATGAGGAAG
    CGGCGGCTGG ACCAGTGCCA GCAGTTCGTG GTCTTCGAGC GAAGCGCCAA GCAGGCTCTG
    GAGTGGATCC ACGACAGCGG GGAGTTCTAC CTCTCCACCC ACACGTCCAC GGGCTCAAGC
    ATCCAGCACA CCCAGGAGCT GCTGAAGGAG CATGAGGACT TCCAGATCAC CGCCAAGCAA
    ACCAAAGAGA GAGTGAAGCT ATTGATACAG CTGGCCGACG GCTTTTGTGA AAAGGGGCAT
    GCCCATGCGG CAGAAATAAA AAAATGTGTC ACTGCCGTGG ATAAGAGGTA CAGAGACTTC
    TCTCTGCGGA TGGAGAAATA CAGGACCTCT TTGGAGAAAG CCCTAGGGAT TTCTTCAGAT
    TCCAACAAAT CGAGCAAAAG TCTTCAGCTG GATATTGTTC CGGCCAGTAT CCCCGGCTCA
    GAGGTGAAAC TCCGAGACGC CGCCCATGAA CTTAATGAGG AAAAGCGGAA ATCTGCCCGC
    AGGAAAGAGT TCATAATGGC TGAGCTAATT CAAACTGAAA AGGCTTACGT AAGAGACCTC
    CGGGAGTGTA TGGATACCTA CCTGTGGGAA ATGACCAGCG GCGTGGAGGA GATCCCCCCG
    GGCATTGTAA ACAAAGAGCT CATCATCTTC GGGAACATGC AGGAAATCTA CGAGTTTCAC
    AATAACATAT TCCTAAAAGA GCTGGAAAAA TATGAGCAGT TGCCGGAGGA CGTGGGACAT
    TGCTTTGTAA CCTGGGCAGA CAAGTTTCAG ATGTATGTCA CATATTGCAA AAATAAGCCT
    GATTCTACTC AGCTGATATT AGAGCATGCA GGATCCTACT TTGATGAAAT ACAGCAGCGG
    CACGGATTAG CCAATTCCAT CTCCTCCTAC CTTATTAAAC CAGTTCAGCG AATAACGAAA
    TATCAGCTCC TTTTAAAAGA GCTGCTCACG TGCTGCGAGG AGGGGAAGGG GGAGATCAAG
    GATGGGCTGG AGGTGATGCT CAGCGTGCCT AAGCGGGCCA ACGATGCCAT GCACCTCAGC
    ATGCTGGAAG GGTTCGATGA GAACATTGAG TCTCAGGGAG AGCTGATCCT GCAGGAATCC
    TTCCAAGTGT GGGACCCCAA AACCTTAATC CGAAAGGGCC GTGAGCGGCA CCTTTTCCTG
    TTTGAGATGT CCTTGGTGTT TAGTAAAGAA GTAAAAGATT CCAGTGGCAG AAGCAAGTAC
    CTTTATAAAA GCAAATTGTT TACCTCAGAG CTGGGTGTCA CAGAGCACGT TGAAGGAGAC
    CCTTGCAAAT TCGCACTGTG GGTGGGGAGG ACACCAACTT CAGATAATAA GATTGTCCTT
    AAGGCCTCCA GTATTGAGAA CAAGCAGGAC TGGATCAAGC ATATCCGGGA GGTGATCCAG
    GAAAGGACCA TCCACCTGAA AGGCGCCCTG AAGGAGCCCA TCCACATCCC CAAAACAGCT
    CCAGCCACGA GACAGAAGGG AAGGAGGGAT GGAGAGGATT TGGACAGCCA AGGAGATGGC
    AGCAGCCAGC CCGATACGAT TTCCATTGCC TCCCGGACGT CTCAGAACAC GCTGGACAGT
    GACAAGCTGT CCGGCGGCTG CGAGCTGACC GTGGTGATCC ACGACTTCAC GGCCTGCAAC
    AGCACCGAGC TCACCATCCG CCGCGGCCAG ACCGTGGAAG TCTTGGAGCG GCCCCATGAC
    AAACCCGACT GGTGCCTGGT GCGGACCACT GACCGGTCGC CTGCGGCCGA GGGCCTGGTC
    CCCTGTGGCT CCCTGTGCAT CGCCCACTCC AGGAGCAGCA TGGAGATGGA GGGCATCTTC
    AACCACAAAG ACTCACTGTC CGTCTCCAGC AACGACGCCA GCCCGCCCGC CTCCGTGGCC
    TCCCTCCAGC CCCACATGGT AGGGGCGCAG AGCTCGCCGG GCCCCAAGCG CCCGGGCAAC
    ACGCTGCGTA AGTGGCTGAC AAGCCCCGTG CGGCGGCTGA GCAGCGGCAA GGCGGATGGG
    CACGTGAAGA AGCTGGCGCA CAAGCACAAG AAGAGCCGCG AGGTCCGCAA GAGCGCCGAC
    GCCGGCTCGC AGAAGGACTC GGACGACAGC GCGGCCACCC CGCAGGACGA GACCGTGGAA
    GAGAGAGGCC GGAACGAGGG CCTGAGCAGT GGCACGCTTT CCAAGTCCTC ATCTTCGGGC
    ATGCAGAGCT GCGGAGAGGA AGAAGGGGAG GAGGGGGCCG ACGCCGTGCC CCTGCCGCCA
    CCCATGGCCA TCCAGCAGCA CAGCCTGCTC CAGCCAGACT CCCAGGACGA CAAGGCCTCT
    TCTCGGTTAT TAGTCCGCCC CACCAGCTCC GAAACACCGA GCGCAGCCGA GCTTGTCAGT
    GCAATCGAGG AACTCGTGAA AAGCAAGATG GCTCTGGAGG ATCGTCCCAG CTCGCTCCTC
    GTCGACCAGG GGGACAGCAG CAGCCCCTCC TTCAACCCTT CAGACAACTC CCTGCTGTCC
    TCCTCCTCAC CCATCGATGA GATGGAAGAG AGGAAGTCCA GCTCTTTAAA GAGACGCCAC
    TACGTTTTAC AAGAATTAGT GGAGACAGAG CGTGACTATG TGCGGGACCT CGGCTGTGTG
    GTGGAGGGCT ACATGGCACT TATGAAAGAA GATGGTGTTC CTGACGACAT GAAAGGCAAA
    GACAAGATTG TGTTTGGCAA CATCCATCAG ATTTACGACT GGCACAGAGA CTTTTTTTTA
    GGAGAGTTGG AGAAGTGCCT TGAAGATCCG GAAAAACTAG GATCCCTTTT TGTTAAACAT
    GAGAGAAGGT TGCACATGTA CATAGTTTAT TGCCAAAATA AACCAAAGTC TGAACACATT
    GTCTCAGAAT ACATTGACAC CTTTTTTGAG GACTTAAAGC AGCGCCTGGG CCACAGGCTG
    CAGCTCACGG ACTTGCTGAT CAAACCCGTT CAGAGGATCA CGAAGTACCA GCTGCTGCTG
    AAGGACTTCC TCAAGTATTC CAAAAAGGCC AGCCTGGATA CATCAGAACT AGAGAGAGCC
    GTGGAGGTCA TGTGCGTCGT CCCGAAGCGC TGCAACGACA TGATGAACGT CGGGAGGCTA
    CAAGGCTTCG ACGGTAAGAT CGTGGCCCAG GGCAAGCTGC TCCTGCAGGA CACGTTCTTG
    GTCACGGACC AGGACACGGG GCTGCTGCCT CGCTGCAAAG AGAGGCGGGT GTTCCTCTTC
    GAGCAGATCG TCATTTTCAG TGAGCCGCTG GATAAAAAAA AGGGCTTCTC CATGCCAGGA
    TTCCTGTTCA AGAACAGTAT CAAGGTGAGC TGCCTTTGCC TGGAGGAAAA CGTGGAAAAT
    GATCCCTGTA AGTTCGCTCT GACATCGCGG ACGGGTGACG TGGTGGAGAC TTTCATCCTG
    CACTCATCCA GTCCAAGTGT CCGGCAGACG TGGATCCATG AAATCAACCA GATTTTAGAA
    AACCAGCGCA ATTTTTTAAA CGCCTTGACA TCACCCATCG AATATCAGAG GAACCACAGC
    GGGGGCGGCG GCAGCGGCGG GGGCGGGGGC AGCAGCGGGG CCCCCAGCGG CGGGGGCGGC
    GGCAGCAACC ACAGCGGCGG TGGGGGCGGC CCCGGTGGCG GCGGCAGCGG CAGCTCGAGC
    AGAAGTAGAC CCTCCCGGAT TCCCCAGCCC GTCAGACACC ACTCCCCCGT GCTGGTCTCC
    TCTGCAGCCT CGAGCCAGGC AGAGGCAGAC AAGATGTCAG GTACGTGCAC CCCGGGCCCG
    CCACCGCCTC CTCCCAGCCG CAGCCCCGCC CCGGAGGCCA GCGCCGGCCC GCCTGGCAGG
    GCGCCCCCCA GCAGCGAGCC CGACGGTCCC GAGCGAGACA CCGAGCCGAT CCCCAAGATG
    AAGGTGCTGG AGAGCCCCCG GAAGTCGTCC GGGGGCGTGC TGCCACAGGA CGGAGCCGCC
    AAGGAGGCAC CGCGGAGCGG CCCGGGCCCC CTTCCGCTGG GCAAGGCCAG GCCCGGGGCC
    CCCTCGCCCC TGCACTCACC TCTGGCCGCC GCCGCCGCGT CCCCCTCTCC GTTCGGCAAG
    GAGCCCTTGC CCCCCAGCAG CCCCCTGCAG AAGGGGGGCT CCTTCTGGAG CTCCGTGCCT
    GCCTCCCCTG CCAGCCGGCC CGGCTCCTTC ACCTTCCCGG GGGACAGCGA CTCCCTGCAG
    CGGCAGGCAC GGCGCCACGC GGCCCCTGGC AAGGATGCGG ACCGCATGAG CACGTGCTCC
    TCGGCCAGCG AGCAGTCCGT GCAGTCCACC CAGAGCAACG GGAGTGAAAG CAGCAGCGGT
    AGCAACATCT CCACCATGTT GGTGACACAC GACTACACGG CAGTGAAGGA GGATGAGATA
    AACGTCTATC AAGGAGAGGT CGTTCAAATT CTGGCCAGCA ATCAACAGAA CATGTTTCTG
    GTGTTCCGAG CCGCCACTGA CCAGTGCCCC GCAGCCGAGG GCTGGATTCC GGGCTTCGTC
    CTGGGGCACA CCAGCGCAGT CATCATGGAG AACCCTGACG GGACTCTCAA GAAGTCAACA
    TCTTGGCACA CAGCACTCCG CTTAAGGAAA AAATCTGAGA AAAAAGATAA AGACGGCAAA
    CGGGAAGGCA AGTTAGAGAA CGGTTACCGG AAGTCTCGGG AAGGACTGAG CAACAAGGTA
    TCTGTGAAGC TTCTCAATCC CAACTACATA TATGACGTTC CCCCAGAATT CGTCATTCCC
    TTGAGTGAGG TCACGTGTGA GGCAGGCGAG ACCGTTGTTC TTCGATGTCG GGTTTGTGGC
    CGCCCCAAGG CCTCCATCAC CTGGAAGGGC CCTGAACACA ACACCTTGAA CAACGACGGT
    CACTACAGCA TCTCCTACAG CGACTTGGGA GAGGCCGCTC TGAAGATCGT GGGTGTGGCC
    GCCGAGGACG ACGGCGTCTA CACGTGCATC GCGGTGAACG ACATGGGGTC AGCCTCGTCT
    TCAGCCAGCC TGAGGGTTCT AGGTCCAGGG AGTGATGGCA TCGTGGTGAC CTGGAAGGAC
    AACTTTGACT GCTTCTACAG CGAGGTGGCC GAGCTCGGCA GGGGCAGATT CTCTGTCGTG
    AAGAAATGTG ATCAGAAAGG CACCAAACGA GCCGTGGCCA CTAAGTTTGT GAACAAGAAG
    CTGATGAAGC GTGACCAGGT CACCCACGAA CTTGGGATCC TGCAGAACCT CCAGCACCCG
    CTGCTGGTGG GCCTCCTGGA CACCTTCGAG ACCCCCACCA GCTACGTGCT GGTCCTGGAG
    ATGGCCGACC AGGGACGGCT CCTGGACTGC GTGGTGCGGT GGGGGAACCT CACGGAGGGG
    AAGATCCGGG CGTACCTGGG GGAGGTTCTG GAAGCCGTGC GATACCTTCA CAACTGCAGG
    ATAGTGCATC TGGACCTAAA GCCTGAGAAC ATCCTCGTGG ATCAGAGCTC AGCCAAGCCG
    ACCATCAAGC TGGCTGACTT CGGAGATGCC GTCCAACTCA ACACGACCTA CCACATCCAC
    CCATTGCTGG GGAACCCCGA GTTCGCAGCC CCCGAGATCA TCCTGGGGAA CCCGGTCTCC
    CTGACCTCAG ACACGTGGAG CGTGGGCGTG CTGGCCTACG TGCTTCTCAG CGGCGTGTCC
    CCCTTCCTGG ACGACAGCGT GGAAGAGACC TGCCTCAACA TTTGCCGCCT GGACTTCAGC
    TTCCCAGAGG ACTACTTCCA AGGAGTGAGC CAGAGGGCCA AGGACTTCGT GTGCTTCCTA
    CTGCACGAGG ACCCCGCCAA GCGCCCCTCG GCTGCGTCGG CCCTCCAGGA GCGGTGGCTG
    CAGGCGGGCC ACGGCCACGG CAAAGGCGCG GGCGTCCTCG ACACGTCCAG ACTGACCTCC
    TTCATTGAGC GGCGCAAACA CCAGAACGAC GTTCGACCTA TCCGTAGCAT GAAAAACTTC
    TTACAGAGCA GGCTTCTGCC TCGAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37677
    Clone ID Related Accession (Same CDS sequence) NM_001102212.1
    Accession Version NM_001102212.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product triple functional domain protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC150056.1. On Aug 21, 2007 this sequence version replaced XM_618492.3. ##Evidence-Data-START## Transcript exon combination :: BC150056.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCGAACG GAATTAAGCC AGTAGATCCG CTGGACTCAC TTTCAGCCGT CAGCACAGCC 
    TACCGCGGCG GCGGGCAGCC TGGCACCTGC AAGTACTGGA TCCTGGACCT AGACTCACTG
    TCCGTCTCCA GCAACGACGC CAGCCCGCCC GCCTCCGTGG CCTCCCTCCA GCCCCACATG
    GTAGGGGCGC AGAGCTCGCC GGGCCCCAAG CGCCCGGGCA ACACGCTGCG TAAGTGGCTG
    ACAAGCCCCG TGCGGCGGCT GAGCAGCGGC AAGGCGGATG GGCACGTGAA GAAGCTGGCG
    CACAAGCACA AGAAGAGCCG CGAGGTCCGC AAGAGCGCCG ACGCCGGCTC GCAGAAGGAC
    TCGGACGACA GCGCGGCCAC CCCGCAGGAC GAGACCGTGG AAGAGAGAGG CCGGAACGAG
    GGCCTGAGCA GTGGCACGCT TTCCAAGTCC TCATCTTCGG GCATGCAGAG CTGCGGAGAG
    GAAGAAGGGG AGGAGGGGGC CGACGCCGTG CCCCTGCCGC CACCCATGGC CATCCAGCAG
    CACAGCCTGC TCCAGCCAGA CTCCCAGGAC GACAAGGCCT CTTCTCGGTT ATTAGTCCGC
    CCCACCAGCT CCGAAACACC GAGCGCAGCC GAGCTTGTCA GTGCAATCGA GGAACTCGTG
    AAAAGCAAGA TGGCTCTGGA GGATCGTCCC AGCTCGCTCC TCGTCGACCA GGGGGACAGC
    AGCAGCCCCT CCTTCAACCC TTCAGACAAC TCCCTGCTGT CCTCCTCCTC ACCCATCGAT
    GAGATGGAAG AGAGGAAGTC CAGCTCTTTA AAGAGACGCC ACTACGTTTT ACAAGAATTA
    GTGGAGACAG AGCGTGACTA TGTGCGGGAC CTCGGCTGTG TGGTGGAGGG CTACATGGCA
    CTTATGAAAG AAGATGGTGT TCCTGACGAC ATGAAAGGCA AAGACAAGAT TGTGTTTGGC
    AACATCCATC AGATTTACGA CTGGCACAGA GACTTTTTTT TAGGAGAGTT GGAGAAGTGC
    CTTGAAGATC CGGAAAAACT AGGATCCCTT TTTGTTAAAC ATGAGAGAAG GTTGCACATG
    TACATAGTTT ATTGCCAAAA TAAACCAAAG TCTGAACACA TTGTCTCAGA ATACATTGAC
    ACCTTTTTTG AGGACTTAAA GCAGCGCCTG GGCCACAGGC TGCAGCTCAC GGACTTGCTG
    ATCAAACCCG TTCAGAGGAT CACGAAGTAC CAGCTGCTGC TGAAGGACTT CCTCAAGTAT
    TCCAAAAAGG CCAGCCTGGA TACATCAGAA CTAGAGAGAG CCGTGGAGGT CATGTGCGTC
    GTCCCGAAGC GCTGCAACGA CATGATGAAC GTCGGGAGGC TACAAGGCTT CGACGGTAAG
    ATCGTGGCCC AGGGCAAGCT GCTCCTGCAG GACACGTTCT TGGTCACGGA CCAGGACACG
    GGGCTGCTGC CTCGCTGCAA AGAGAGGCGG GTGTTCCTCT TCGAGCAGAT CGTCATTTTC
    AGTGAGCCGC TGGATAAAAA AAAGGGCTTC TCCATGCCAG GATTCCTGTT CAAGAACAGT
    ATCAAGGTGA GCTGCCTTTG CCTGGAGGAA AACGTGGAAA ATGATCCCTG TAAGTTCGCT
    CTGACATCGC GGACGGGTGA CGTGGTGGAG ACTTTCATCC TGCACTCATC CAGTCCAAGT
    GTCCGGCAGA CGTGGATCCA TGAAATCAAC CAGATTTTAG AAAACCAGCG CAATTTTTTA
    AACGCCTTGA CATCACCCAT CGAATATCAG AGGAACCACA GCGGGGGCGG CGGCAGCGGC
    GGGGGCGGGG GCAGCAGCGG GGCCCCCAGC GGCGGGGGCG GCGGCAGCAA CCACAGCGGC
    GGTGGGGGCG GCCCCGGTGG CGGCGGCAGC GGCAGCTCGA GCAGAAGTAG ACCCTCCCGG
    ATTCCCCAGC CCGTCAGACA CCACTCCCCC GTGCTGGTCT CCTCTGCAGC CTCGAGCCAG
    GCAGAGGCAG ACAAGATGTC AGGTACGTGC ACCCCGGGCC CGCCACCGCC TCCTCCCAGC
    CGCAGCCCCG CCCCGGAGGC CAGCGCCGGC CCGCCTGGCA GGGCGCCCCC CAGCAGCGAG
    CCCGACGGTC CCGAGCGAGA CACCGAGCCG ATCCCCAAGA TGAAGGTGCT GGAGAGCCCC
    CGGAAGTCGT CCGGGGGCGT GCTGCCACAG GACGGAGCCG CCAAGGAGGC ACCGCGGAGC
    GGCCCGGGCC CCCTTCCGCT GGGCAAGGCC AGGCCCGGGG CCCCCTCGCC CCTGCACTCA
    CCTCTGGCCG CCGCCGCCGC GTCCCCCTCT CCGTTCGGCA AGGAGCCCTT GCCCCCCAGC
    AGCCCCCTGC AGAAGGGGGG CTCCTTCTGG AGCTCCGTGC CTGCCTCCCC TGCCAGCCGG
    CCCGGCTCCT TCACCTTCCC GGGGGACAGC GACTCCCTGC AGCGGCAGGC ACGGCGCCAC
    GCGGCCCCTG GCAAGGATGC GGACCGCATG AGCACGTGCT CCTCGGCCAG CGAGCAGTCC
    GTGCAGTCCA CCCAGAGCAA CGGGAGTGAA AGCAGCAGCG GTAGCAACAT CTCCACCATG
    TTGGTGACAC ACGACTACAC GGCAGTGAAG GAGGATGAGA TAAACGTCTA TCAAGGAGAG
    GTCGTTCAAA TTCTGGCCAG CAATCAACAG AACATGTTTC TGGTGTTCCG AGCCGCCACT
    GACCAGTGCC CCGCAGCCGA GGGCTGGATT CCGGGCTTCG TCCTGGGGCA CACCAGCGCA
    GTCATCATGG AGAACCCTGA CGGGACTCTC AAGAAGTCAA CATCTTGGCA CACAGCACTC
    CGCTTAAGGA AAAAATCTGA GAAAAAAGAT AAAGACGGCA AACGGGAAGG CAAGTTAGAG
    AACGGTTACC GGAAGTCTCG GGAAGGACTG AGCAACAAGG TATCTGTGAA GCTTCTCAAT
    CCCAACTACA TATATGACGT TCCCCCAGAA TTCGTCATTC CCTTGAGTGA GGTCACGTGT
    GAGGCAGGCG AGACCGTTGT TCTTCGATGT CGGGTTTGTG GCCGCCCCAA GGCCTCCATC
    ACCTGGAAGG GCCCTGAACA CAACACCTTG AACAACGACG GTCACTACAG CATCTCCTAC
    AGCGACTTGG GAGAGGCCGC TCTGAAGATC GTGGGTGTGG CCGCCGAGGA CGACGGCGTC
    TACACGTGCA TCGCGGTGAA CGACATGGGG TCAGCCTCGT CTTCAGCCAG CCTGAGGGTT
    CTAGGTCCAG GGAGTGATGG CATCGTGGTG ACCTGGAAGG ACAACTTTGA CTGCTTCTAC
    AGCGAGGTGG CCGAGCTCGG CAGGGGCAGA TTCTCTGTCG TGAAGAAATG TGATCAGAAA
    GGCACCAAAC GAGCCGTGGC CACTAAGTTT GTGAACAAGA AGCTGATGAA GCGTGACCAG
    GTCACCCACG AACTTGGGAT CCTGCAGAAC CTCCAGCACC CGCTGCTGGT GGGCCTCCTG
    GACACCTTCG AGACCCCCAC CAGCTACGTG CTGGTCCTGG AGATGGCCGA CCAGGGACGG
    CTCCTGGACT GCGTGGTGCG GTGGGGGAAC CTCACGGAGG GGAAGATCCG GGCGTACCTG
    GGGGAGGTTC TGGAAGCCGT GCGATACCTT CACAACTGCA GGATAGTGCA TCTGGACCTA
    AAGCCTGAGA ACATCCTCGT GGATCAGAGC TCAGCCAAGC CGACCATCAA GCTGGCTGAC
    TTCGGAGATG CCGTCCAACT CAACACGACC TACCACATCC ACCCATTGCT GGGGAACCCC
    GAGTTCGCAG CCCCCGAGAT CATCCTGGGG AACCCGGTCT CCCTGACCTC AGACACGTGG
    AGCGTGGGCG TGCTGGCCTA CGTGCTTCTC AGCGGCGTGT CCCCCTTCCT GGACGACAGC
    GTGGAAGAGA CCTGCCTCAA CATTTGCCGC CTGGACTTCA GCTTCCCAGA GGACTACTTC
    CAAGGAGTGA GCCAGAGGGC CAAGGACTTC GTGTGCTTCC TACTGCACGA GGACCCCGCC
    AAGCGCCCCT CGGCTGCGTC GGCCCTCCAG GAGCGGTGGC TGCAGGCGGG CCACGGCCAC
    GGCAAAGGCG CGGGCGTCCT CGACACGTCC AGACTGACCT CCTTCATTGA GCGGCGCAAA
    CACCAGAACG ACGTTCGACC TATCCGTAGC ATGAAAAACT TCTTACAGAG CAGGCTTCTG
    CCTCGAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001095682.1
    CDS140..4351
    Translation

    Target ORF information:

    RefSeq Version NM_001102212.1
    Organism Bos taurus(cattle)
    Definition Bos taurus trio Rho guanine nucleotide exchange factor (TRIO), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102212.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCCGAACG GAATTAAGCC AGTAGATCCG CTGGACTCAC TTTCAGCCGT CAGCACAGCC 
    TACCGCGGCG GCGGGCAGCC TGGCACCTGC AAGTACTGGA TCCTGGACCT AGACTCACTG
    TCCGTCTCCA GCAACGACGC CAGCCCGCCC GCCTCCGTGG CCTCCCTCCA GCCCCACATG
    GTAGGGGCGC AGAGCTCGCC GGGCCCCAAG CGCCCGGGCA ACACGCTGCG TAAGTGGCTG
    ACAAGCCCCG TGCGGCGGCT GAGCAGCGGC AAGGCGGATG GGCACGTGAA GAAGCTGGCG
    CACAAGCACA AGAAGAGCCG CGAGGTCCGC AAGAGCGCCG ACGCCGGCTC GCAGAAGGAC
    TCGGACGACA GCGCGGCCAC CCCGCAGGAC GAGACCGTGG AAGAGAGAGG CCGGAACGAG
    GGCCTGAGCA GTGGCACGCT TTCCAAGTCC TCATCTTCGG GCATGCAGAG CTGCGGAGAG
    GAAGAAGGGG AGGAGGGGGC CGACGCCGTG CCCCTGCCGC CACCCATGGC CATCCAGCAG
    CACAGCCTGC TCCAGCCAGA CTCCCAGGAC GACAAGGCCT CTTCTCGGTT ATTAGTCCGC
    CCCACCAGCT CCGAAACACC GAGCGCAGCC GAGCTTGTCA GTGCAATCGA GGAACTCGTG
    AAAAGCAAGA TGGCTCTGGA GGATCGTCCC AGCTCGCTCC TCGTCGACCA GGGGGACAGC
    AGCAGCCCCT CCTTCAACCC TTCAGACAAC TCCCTGCTGT CCTCCTCCTC ACCCATCGAT
    GAGATGGAAG AGAGGAAGTC CAGCTCTTTA AAGAGACGCC ACTACGTTTT ACAAGAATTA
    GTGGAGACAG AGCGTGACTA TGTGCGGGAC CTCGGCTGTG TGGTGGAGGG CTACATGGCA
    CTTATGAAAG AAGATGGTGT TCCTGACGAC ATGAAAGGCA AAGACAAGAT TGTGTTTGGC
    AACATCCATC AGATTTACGA CTGGCACAGA GACTTTTTTT TAGGAGAGTT GGAGAAGTGC
    CTTGAAGATC CGGAAAAACT AGGATCCCTT TTTGTTAAAC ATGAGAGAAG GTTGCACATG
    TACATAGTTT ATTGCCAAAA TAAACCAAAG TCTGAACACA TTGTCTCAGA ATACATTGAC
    ACCTTTTTTG AGGACTTAAA GCAGCGCCTG GGCCACAGGC TGCAGCTCAC GGACTTGCTG
    ATCAAACCCG TTCAGAGGAT CACGAAGTAC CAGCTGCTGC TGAAGGACTT CCTCAAGTAT
    TCCAAAAAGG CCAGCCTGGA TACATCAGAA CTAGAGAGAG CCGTGGAGGT CATGTGCGTC
    GTCCCGAAGC GCTGCAACGA CATGATGAAC GTCGGGAGGC TACAAGGCTT CGACGGTAAG
    ATCGTGGCCC AGGGCAAGCT GCTCCTGCAG GACACGTTCT TGGTCACGGA CCAGGACACG
    GGGCTGCTGC CTCGCTGCAA AGAGAGGCGG GTGTTCCTCT TCGAGCAGAT CGTCATTTTC
    AGTGAGCCGC TGGATAAAAA AAAGGGCTTC TCCATGCCAG GATTCCTGTT CAAGAACAGT
    ATCAAGGTGA GCTGCCTTTG CCTGGAGGAA AACGTGGAAA ATGATCCCTG TAAGTTCGCT
    CTGACATCGC GGACGGGTGA CGTGGTGGAG ACTTTCATCC TGCACTCATC CAGTCCAAGT
    GTCCGGCAGA CGTGGATCCA TGAAATCAAC CAGATTTTAG AAAACCAGCG CAATTTTTTA
    AACGCCTTGA CATCACCCAT CGAATATCAG AGGAACCACA GCGGGGGCGG CGGCAGCGGC
    GGGGGCGGGG GCAGCAGCGG GGCCCCCAGC GGCGGGGGCG GCGGCAGCAA CCACAGCGGC
    GGTGGGGGCG GCCCCGGTGG CGGCGGCAGC GGCAGCTCGA GCAGAAGTAG ACCCTCCCGG
    ATTCCCCAGC CCGTCAGACA CCACTCCCCC GTGCTGGTCT CCTCTGCAGC CTCGAGCCAG
    GCAGAGGCAG ACAAGATGTC AGGTACGTGC ACCCCGGGCC CGCCACCGCC TCCTCCCAGC
    CGCAGCCCCG CCCCGGAGGC CAGCGCCGGC CCGCCTGGCA GGGCGCCCCC CAGCAGCGAG
    CCCGACGGTC CCGAGCGAGA CACCGAGCCG ATCCCCAAGA TGAAGGTGCT GGAGAGCCCC
    CGGAAGTCGT CCGGGGGCGT GCTGCCACAG GACGGAGCCG CCAAGGAGGC ACCGCGGAGC
    GGCCCGGGCC CCCTTCCGCT GGGCAAGGCC AGGCCCGGGG CCCCCTCGCC CCTGCACTCA
    CCTCTGGCCG CCGCCGCCGC GTCCCCCTCT CCGTTCGGCA AGGAGCCCTT GCCCCCCAGC
    AGCCCCCTGC AGAAGGGGGG CTCCTTCTGG AGCTCCGTGC CTGCCTCCCC TGCCAGCCGG
    CCCGGCTCCT TCACCTTCCC GGGGGACAGC GACTCCCTGC AGCGGCAGGC ACGGCGCCAC
    GCGGCCCCTG GCAAGGATGC GGACCGCATG AGCACGTGCT CCTCGGCCAG CGAGCAGTCC
    GTGCAGTCCA CCCAGAGCAA CGGGAGTGAA AGCAGCAGCG GTAGCAACAT CTCCACCATG
    TTGGTGACAC ACGACTACAC GGCAGTGAAG GAGGATGAGA TAAACGTCTA TCAAGGAGAG
    GTCGTTCAAA TTCTGGCCAG CAATCAACAG AACATGTTTC TGGTGTTCCG AGCCGCCACT
    GACCAGTGCC CCGCAGCCGA GGGCTGGATT CCGGGCTTCG TCCTGGGGCA CACCAGCGCA
    GTCATCATGG AGAACCCTGA CGGGACTCTC AAGAAGTCAA CATCTTGGCA CACAGCACTC
    CGCTTAAGGA AAAAATCTGA GAAAAAAGAT AAAGACGGCA AACGGGAAGG CAAGTTAGAG
    AACGGTTACC GGAAGTCTCG GGAAGGACTG AGCAACAAGG TATCTGTGAA GCTTCTCAAT
    CCCAACTACA TATATGACGT TCCCCCAGAA TTCGTCATTC CCTTGAGTGA GGTCACGTGT
    GAGGCAGGCG AGACCGTTGT TCTTCGATGT CGGGTTTGTG GCCGCCCCAA GGCCTCCATC
    ACCTGGAAGG GCCCTGAACA CAACACCTTG AACAACGACG GTCACTACAG CATCTCCTAC
    AGCGACTTGG GAGAGGCCGC TCTGAAGATC GTGGGTGTGG CCGCCGAGGA CGACGGCGTC
    TACACGTGCA TCGCGGTGAA CGACATGGGG TCAGCCTCGT CTTCAGCCAG CCTGAGGGTT
    CTAGGTCCAG GGAGTGATGG CATCGTGGTG ACCTGGAAGG ACAACTTTGA CTGCTTCTAC
    AGCGAGGTGG CCGAGCTCGG CAGGGGCAGA TTCTCTGTCG TGAAGAAATG TGATCAGAAA
    GGCACCAAAC GAGCCGTGGC CACTAAGTTT GTGAACAAGA AGCTGATGAA GCGTGACCAG
    GTCACCCACG AACTTGGGAT CCTGCAGAAC CTCCAGCACC CGCTGCTGGT GGGCCTCCTG
    GACACCTTCG AGACCCCCAC CAGCTACGTG CTGGTCCTGG AGATGGCCGA CCAGGGACGG
    CTCCTGGACT GCGTGGTGCG GTGGGGGAAC CTCACGGAGG GGAAGATCCG GGCGTACCTG
    GGGGAGGTTC TGGAAGCCGT GCGATACCTT CACAACTGCA GGATAGTGCA TCTGGACCTA
    AAGCCTGAGA ACATCCTCGT GGATCAGAGC TCAGCCAAGC CGACCATCAA GCTGGCTGAC
    TTCGGAGATG CCGTCCAACT CAACACGACC TACCACATCC ACCCATTGCT GGGGAACCCC
    GAGTTCGCAG CCCCCGAGAT CATCCTGGGG AACCCGGTCT CCCTGACCTC AGACACGTGG
    AGCGTGGGCG TGCTGGCCTA CGTGCTTCTC AGCGGCGTGT CCCCCTTCCT GGACGACAGC
    GTGGAAGAGA CCTGCCTCAA CATTTGCCGC CTGGACTTCA GCTTCCCAGA GGACTACTTC
    CAAGGAGTGA GCCAGAGGGC CAAGGACTTC GTGTGCTTCC TACTGCACGA GGACCCCGCC
    AAGCGCCCCT CGGCTGCGTC GGCCCTCCAG GAGCGGTGGC TGCAGGCGGG CCACGGCCAC
    GGCAAAGGCG CGGGCGTCCT CGACACGTCC AGACTGACCT CCTTCATTGA GCGGCGCAAA
    CACCAGAACG ACGTTCGACC TATCCGTAGC ATGAAAAACT TCTTACAGAG CAGGCTTCTG
    CCTCGAGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228048
    Clone ID Related Accession (Same CDS sequence) XM_024981340.1
    Accession Version XM_024981340.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product triple functional domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGTCTCCTA CATTGGCAGG GTTTCGAAAA AACGATGAAA TGAAAGCTAT GGATGTTTTG 
    CCCATTTTAA AGGAAAAAGT TGCATACCTT TCAGGTGGCA GAGACAAGCG CGGGGGTCCC
    ATCCTCACCT TCCCGGCCCG CAGCAACCAT GACAGAATAC GGCAGGAGGA CCTCCGGAGG
    CTCATCTCCT ACCTGGCCTG TATCCCCAGT GAAGAAGTCT GCAAGCGAGG GTTCACGGTG
    ATTGTGGACA TGCGTGGGTC CAAGTGGGAC TCCATCAAGC CGCTGCTGAA GATCTTACAA
    GAGTCCTTCC CCTGCTGCAT CCATGTCGCG CTGATAATCA AGCCAGACAA CTTTTGGCAG
    AAACAGAGGA CTAATTTTGG CAGTTCTAAA TTTGAATTTG AGACAAATAT GGTCTCTTTA
    GAAGGCCTTA CCAAAGTAGT TGATCCTTCT CAGCTAACGC CCGAGTTTGA CGGCTGCTTG
    GAGTACAACC ACGAAGAATG GATTGAGATC CGACTTGCTT TTGAAGACTA CATTAGCAAC
    GCGACCCACA TGCTCTCTCG GCTGGAGGAG CTCCAGGACA TCCTCGCCAA GAAGGAGTTG
    CCCCAGGATT TGGAGGGGGC GCGGAACATG ATCGAGGAGC ATTCCCAGCT GAAGAAGAAG
    GTGATTAAGG CCCCCATAGA GGACCTGGAC CTAGAGGGAC AGAAGCTGCT GCAGAGGATT
    CAGAGCAGCG AGAGCTTTCC CAAAAAGAAC TCGGGCTCAG GCAACGCAGA CCTGCAGAGC
    CTCTTACCCA AGGTGTCTGC CATGCTGGAC CGGCTGCACT CGACGCGGCA GCATCTGCAT
    CAGATGTGGC ACGTGCGCAA GCTGAAGCTG GACCAGTGCT TTCAGCTGAG GCTGTTCGAG
    CAGGATGCTG AGAAGATGTT TGACTGGATC ACACACAACA AGGGCCTGTT TCTGAACAGC
    TACACTGAGA TTGGGACCAG CCACCCTCAT GCCATGGAGC TCCAGACCCA GCACAACCAC
    TTTGCCATGA ACTGCATGAA CGTGTATGTG AACATCAACC GCATCATGTC GGTGGCCAAC
    CGCCTGGTGG AGTCGGGCCA CTATGCCTCC CAGCAGATCA AGCAGATCGC CAACCAGCTG
    GAGCAGGAGT GGAAGGCCTT CGCGGCTGCC CTGGACGAGC GTAGCACCTT GCTGGACATG
    TCATCTGTCT TCCACCAGAA GGCTGAGAAG TATATGAGCA ATGTGGACTC GTGGTGCAAG
    GCCTGCGGCG AGGTGGACCT GCCCTCGGAG CTTCAGGACC TGGAGGACGC TATTCACCAC
    CACCAGGGCG TGTACGAGCA CGTCACTCTT GCCTACTCCG AGGTGAGCCA GGACGGGAAG
    TCCCTGCTCG ACAAGCTCCA GCGGCCCCTG ACGCCCGGCA GCTCTGACTC GCTCACGGCC
    TCGGCCAACT ACTCGAAGGC CGTGCACCAC GTCCTGGACG TCATCCATGA GGTGCTGCAC
    CACCAGCGGC AGCTGGAGAA CATCTGGCAG CACCGCAAGG TGCGGCTCCA CCAGCGGCTG
    CAGCTGTGCG TCTTCCAGCA GGACGTCCAG CAGGTGCTGG ACTGGATCGA GAACCATGGA
    GAAGCATTTC TGAGCAAACA CACAGGTGTA GGGAAATCGC TCCATCGGGC CAGGGCTTTG
    CAGAAACGTC ATGAGGATTT TGAAGAAGTA GCACAGAACA CGTACACCAA TGCAGATAAG
    TTACTTGAGG CAGCCGAGCA GCTGGCCCAG ACGGGAGAAT GTGACCCGGA AGAGATCTAT
    CAGGCTGCCC ATCAGCTCGA GGACCGGATA CAGGATTTTG TGCGGCGCGT GGAGCAGCGG
    AAGATCCTCC TGGACATGTC GGTGTCTTTT CACACGCATG TGAAAGAGCT GTGGACATGG
    CTGGAGGAGC TGCAGAAGGA GCTGCTGGAC GACGTGTACG CCGAGTCCGT GGAGGCCGTG
    CAGGACCTCA GCAAGCGCTT CGGCCAGCAG CAGCAGACCA CCCTGCAGGT GACTGTGAAC
    GTCATCAAGG AAGGCGAGGA CCTGATCCAG CAGCTGAGGG ACTCAGCCAT CTCCAGCAAC
    AAGACGCCGC ACAACAGCTC CATCAGCCAC ATCGAAACGG TGCTGCAGCA GCTGGACGAG
    GCCCAGGCGC AGATGGAGGA GCTCTTCCAG GAGCGCAAGA TCAAGCTGGA GCTCTTCCTG
    CAGCTGCGCA TCTTCGAGCG GGACGCCATC GATATCATCT CAGACCTGGA GTCTTGGAAC
    GATGAGCTTT CTCAGCAGAT GAACGACTTC GACACGGAGG ACCTCACGAT CGCGGAGCAG
    CGCCTGCAGC ACCACGCGGA CAAGGCCCTG ACCATGAACA ACCTGACCTT CGACGTCATC
    CACCAGGGGC AGGACCTGCT GCAGTACGTC AACGAGGTGC AGGCCTCCGG TGTGGAGCTG
    CTTTGCGACA GAGATGTGGA CATGGCCACG CGGGTCCAGG ACCTCCTGGA GTTTCTGCAC
    GAGAAGCAGC AGGAACTGGA CCTCGCAGCT GAGCAGCACC GGAAGCACCT GGAGCAGTGC
    GTGCAGCTGC GCCACCTGCA GGCCGAGGTC AAGCAGGTGC TGGGCTGGAT CCGCAATGGA
    GAGTCCATGC TGAACGCCGG GCTCATCGCG GCCAGCTCGC TGCAGGAGGC AGAGCAGCTG
    CAGAGGGAGC ACGAGCAGTT CCAGCACGCC ATCGAGAAGA CACACCAGAG CGCCCTGCAG
    GTCCAGCAGA AGGCCGAGGC CATGCTGCAG GCCAATCACT ACGACATGGA CATGATCCGG
    GACTGTGCCG AGAAGGTCGC CTCACACTGG CAGCAGCTCA TGCTCAAGAT GGAAGACCGG
    CTGAAGCTGG TGAACGCGTC CGTGGCCTTC TACAAGACCT CAGAGCAGGT CTGCAGCGTC
    CTGGAGAGCC TGGAGCAGGA GTACAAGAGG GAAGAGGACT GGTGCGGAGG GGCCGACAAG
    CTGGGCCCCA ACTCCGAGAC GGACCACGTC ACCCCCATGA TCAGCAAGCA CCTCGAACAG
    AAGGAAGCAT TCCTGAAGGC TTGCACGCTT GCCAGGAGGA ACGCGGACGT CTTCCTGAAG
    TACCTGCACA GGAACAGCGT GAACATGCCA GGCATGGTGA CGCACATCAA GGCCCCCGAG
    CAGCAAGTGA AAAATATCTT GAACGAGCTC TTCCAGCGGG AGAACAGGGT GCTGCACTAC
    TGGACCATGA GGAAGCGGCG GCTGGACCAG TGCCAGCAGT TCGTGGTCTT CGAGCGAAGC
    GCCAAGCAGG CTCTGGAGTG GATCCACGAC AGCGGGGAGT TCTACCTCTC CACCCACACG
    TCCACGGGCT CAAGCATCCA GCACACCCAG GAGCTGCTGA AGGAGCATGA GGACTTCCAG
    ATCACCGCCA AGCAAACCAA AGAGAGAGTG AAGCTATTGA TACAGCTGGC CGACGGCTTT
    TGTGAAAAGG GGCATGCCCA TGCGGCAGAA ATAAAAAAAT GTGTCACTGC CGTGGATAAG
    AGGTACAGAG ACTTCTCTCT GCGGATGGAG AAATACAGGA CCTCTTTGGA GAAAGCCCTA
    GGGATTTCTT CAGATTCCAA CAAATCGAGC AAAAGTCTTC AGCTGGATAT TGTTCCGGCC
    AGTATCCCCG GCTCAGAGGT GAAACTCCGA GACGCCGCCC ATGAACTTAA TGAGGAAAAG
    CGGAAATCTG CCCGCAGGAA AGAGTTCATA ATGGCTGAGC TAATTCAAAC TGAAAAGGCT
    TACGTAAGAG ACCTCCGGGA GTGTATGGAT ACCTACCTGT GGGAAATGAC CAGCGGCGTG
    GAGGAGATCC CCCCGGGCAT TGTAAACAAA GAGCTCATCA TCTTCGGGAA CATGCAGGAA
    ATCTACGAGT TTCACAATAA CATATTCCTA AAAGAGCTGG AAAAATATGA GCAGTTGCCG
    GAGGACGTGG GACATTGCTT TGTAACCTGG GCAGACAAGT TTCAGATGTA TGTCACATAT
    TGCAAAAATA AGCCTGATTC TACTCAGCTG ATATTAGAGC ATGCAGGATC CTACTTTGAT
    GAAATACAGC AGCGGCACGG ATTAGCCAAT TCCATCTCCT CCTACCTTAT TAAACCAGTT
    CAGCGAATAA CGAAATATCA GCTCCTTTTA AAAGAGCTGC TCACGTGCTG CGAGGAGGGG
    AAGGGGGAGA TCAAGGATGG GCTGGAGGTG ATGCTCAGCG TGCCTAAGCG GGCCAACGAT
    GCCATGCACC TCAGCATGCT GGAAGGGTTC GATGAGAACA TTGAGTCTCA GGGAGAGCTG
    ATCCTGCAGG AATCCTTCCA AGTGTGGGAC CCCAAAACCT TAATCCGAAA GGGCCGTGAG
    CGGCACCTTT TCCTGTTTGA GATGTCCTTG GTGTTTAGTA AAGAAGTAAA AGATTCCAGT
    GGCAGAAGCA AGTACCTTTA TAAAAGCAAA TTGTTTACCT CAGAGCTGGG TGTCACAGAG
    CACGTTGAAG GAGACCCTTG CAAATTCGCA CTGTGGGTGG GGAGGACACC AACTTCAGAT
    AATAAGATTG TCCTTAAGGC CTCCAGTATT GAGAACAAGC AGGACTGGAT CAAGCATATC
    CGGGAGGTGA TCCAGGAAAG GACCATCCAC CTGAAAGGCG CCCTGAAGGA GCCCATCCAC
    ATCCCCAAAA CAGCTCCAGC CACGAGACAG AAGGGAAGGA GGGATGGAGA GGATTTGGAC
    AGCCAAGGAG ATGGCAGCAG CCAGCCCGAT ACGATTTCCA TTGCCTCCCG GACGTCTCAG
    AACACGCTGG ACAGTGACAA GCTGTCCGGC GGCTGCGAGC TGACCGTGGT GATCCACGAC
    TTCACGGCCT GCAACAGCAC CGAGCTCACC ATCCGCCGCG GCCAGACCGT GGAAGTCTTG
    GAGCGGCCCC ATGACAAACC CGACTGGTGC CTGGTGCGGA CCACTGACCG GTCGCCTGCG
    GCCGAGGGCC TGGTCCCCTG TGGCTCCCTG TGCATCGCCC ACTCCAGGAG CAGCATGGAG
    ATGGAGGGCA TCTTCAACCA CAAAGACTCA CTGTCCGTCT CCAGCAACGA CGCCAGCCCG
    CCCGCCTCCG TGGCCTCCCT CCAGCCCCAC ATGGTAGGGG CGCAGAGCTC GCCGGGCCCC
    AAGCGCCCGG GCAACACGCT GCGTAAGTGG CTGACAAGCC CCGTGCGGCG GCTGAGCAGC
    GGCAAGGCGG ATGGGCACGT GAAGAAGCTG GCGCACAAGC ACAAGAAGAG CCGCGAGGTC
    CGCAAGAGCG CCGACGCCGG CTCGCAGAAG GACTCGGACG ACAGCGCGGC CACCCCGCAG
    GACGAGACCG TGGAAGAGAG AGGCCGGAAC GAGGGCCTGA GCAGTGGCAC GCTTTCCAAG
    TCCTCATCTT CGGGCATGCA GAGCTGCGGA GAGGAAGAAG GGGAGGAGGG GGCCGACGCC
    GTGCCCCTGC CGCCACCCAT GGCCATCCAG CAGCACAGCC TGCTCCAGCC AGACTCCCAG
    GACGACAAGG CCTCTTCTCG GTTATTAGTC CGCCCCACCA GCTCCGAAAC ACCGAGCGCA
    GCCGAGCTTG TCAGTGCAAT CGAGGAACTC GTGAAAAGCA AGATGGCTCT GGAGGATCGT
    CCCAGCTCGC TCCTCGTCGA CCAGGGGGAC AGCAGCAGCC CCTCCTTCAA CCCTTCAGAC
    AACTCCCTGC TGTCCTCCTC CTCACCCATC GATGAGATGG AAGAGAGGAA GTCCAGCTCT
    TTAAAGAGAC GCCACTACGT TTTACAAGAA TTAGTGGAGA CAGAGCGTGA CTATGTGCGG
    GACCTCGGCT GTGTGGTGGA GGGCTACATG GCACTTATGA AAGAAGATGG TGTTCCTGAC
    GACATGAAAG GCAAAGACAA GATTGTGTTT GGCAACATCC ATCAGATTTA CGACTGGCAC
    AGAGACTTTT TTTTAGGAGA GTTGGAGAAG TGCCTTGAAG ATCCGGAAAA ACTAGGATCC
    CTTTTTGTTA AACATGAGAG AAGGTTGCAC ATGTACATAG TTTATTGCCA AAATAAACCA
    AAGTCTGAAC ACATTGTCTC AGAATACATT GACACCTTTT TTGAGGACTT AAAGCAGCGC
    CTGGGCCACA GGCTGCAGCT CACGGACTTG CTGATCAAAC CCGTTCAGAG GATCACGAAG
    TACCAGCTGC TGCTGAAGGA CTTCCTCAAG TATTCCAAAA AGGCCAGCCT GGATACATCA
    GAACTAGAGA GAGCCGTGGA GGTCATGTGC GTCGTCCCGA AGCGCTGCAA CGACATGATG
    AACGTCGGGA GGCTACAAGG CTTCGACGGT AAGATCGTGG CCCAGGGCAA GCTGCTCCTG
    CAGGACACGT TCTTGGTCAC GGACCAGGAC ACGGGGCTGC TGCCTCGCTG CAAAGAGAGG
    CGGGTGTTCC TCTTCGAGCA GATCGTCATT TTCAGTGAGC CGCTGGATAA AAAAAAGGGC
    TTCTCCATGC CAGGATTCCT GTTCAAGAAC AGTATCAAGG TGAGCTGCCT TTGCCTGGAG
    GAAAACGTGG AAAATGATCC CTGTAAGTTC GCTCTGACAT CGCGGACGGG TGACGTGGTG
    GAGACTTTCA TCCTGCACTC ATCCAGTCCA AGTGTCCGGC AGACGTGGAT CCATGAAATC
    AACCAGATTT TAGAAAACCA GCGCAATTTT TTAAACGCCT TGACATCACC CATCGAATAT
    CAGAGGAACC ACAGCGGGGG CGGCGGCAGC GGCGGGGGCG GGGGCAGCAG CGGGGCCCCC
    AGCGGCGGGG GCGGCGGCAG CAACCACAGC GGCGGTGGGG GCGGCCCCGG TGGCGGCGGC
    AGCGGCAGCT CGAGCAGAAG TAGACCCTCC CGGATTCCCC AGCCCGTCAG ACACCACTCC
    CCCGTGCTGG TCTCCTCTGC AGCCTCGAGC CAGGCAGAGG CAGACAAGAT GTCAGGTACG
    TGCACCCCGG GCCCGCCACC GCCTCCTCCC AGCCGCAGCC CCGCCCCGGA GGCCAGCGCC
    GGCCCGCCTG GCAGGGCGCC CCCCAGCAGC GAGCCCGACG GTCCCGAGCG AGACACCGAG
    CCGATCCCCA AGATGAAGGT GCTGGAGAGC CCCCGGAAGT CGTCCGGGGG CGTGCTGCCA
    CAGGACGGAG CCGCCAAGGA GGCACCGCGG AGCGGCCCGG GCCCCCTTCC GCTGGGCAAG
    GCCAGGCCCG GGGCCCCCTC GCCCCTGCAC TCACCTCTGG CCGCCGCCGC CGCGTCCCCC
    TCTCCGTTCG GCAAGGAGCC CTTGCCCCCC AGCAGCCCCC TGCAGAAGGG GGGCTCCTTC
    TGGAGCTCCG TGCCTGCCTC CCCTGCCAGC CGGCCCGGCT CCTTCACCTT CCCGGGGGAC
    AGCGACTCCC TGCAGCGGCA GGCACGGCGC CACGCGGCCC CTGGCAAGGA TGCGGACCGC
    ATGAGCACGT GCTCCTCGGC CAGCGAGCAG TCCGTGCAGT CCACCCAGAG CAACGGGAGT
    GAAAGCAGCA GCGGTAGCAA CATCTCCACC ATGTTGGTGA CACACGACTA CACGGCAGTG
    AAGGAGGATG AGATAAACGT CTATCAAGGA GAGGTCGTTC AAATTCTGGC CAGCAATCAA
    CAGAACATGT TTCTGGTGTT CCGAGCCGCC ACTGACCAGT GCCCCGCAGC CGAGGGCTGG
    ATTCCGGGCT TCGTCCTGGG GCACACCAGC GCAGTCATCA TGGAGAACCC TGACGGGACT
    CTCAAGAAGT CAACATCTTG GCACACAGCA CTCCGCTTAA GGAAAAAATC TGAGAAAAAA
    GATAAAGACG GCAAACGGGA AGGCAAGTTA GAGAACGGTT ACCGGAAGTC TCGGGAAGGA
    CTGAGCAACA AGGTATCTGT GAAGCTTCTC AATCCCAACT ACATATATGA CGTTCCCCCA
    GAATTCGTCA TTCCCTTGAG TGAGGTCACG TGTGAGGCAG GCGAGACCGT TGTTCTTCGA
    TGTCGGGTTT GTGGCCGCCC CAAGGCCTCC ATCACCTGGA AGGGCCCTGA ACACAACACC
    TTGAACAACG ACGGTCACTA CAGCATCTCC TACAGCGACT TGGGAGAGGC CGCTCTGAAG
    ATCGTGGGTG TGGCCGCCGA GGACGACGGC GTCTACACGT GCATCGCGGT GAACGACATG
    GGGTCAGCCT CGTCTTCAGC CAGCCTGAGG GTTCTAGGTC CAGGGAGTGA TGGCATCGTG
    GTGACCTGGA AGGACAACTT TGACTGCTTC TACAGCGAGG TGGCCGAGCT CGGCAGGGGC
    AGATTCTCTG TCGTGAAGAA ATGTGATCAG AAAGGCACCA AACGAGCCGT GGCCACTAAG
    TTTGTGAACA AGAAGCTGAT GAAGCGTGAC CAGGTCACCC ACGAACTTGG GATCCTGCAG
    AACCTCCAGC ACCCGCTGCT GGTGGGCCTC CTGGACACCT TCGAGACCCC CACCAGCTAC
    GTGCTGGTCC TGGAGATGGC CGACCAGGGA CGGCTCCTGG ACTGCGTGGT GCGGTGGGGG
    AACCTCACGG AGGGGAAGAT CCGGGCGTAC CTGGGGGAGG TTCTGGAAGC CGTGCGATAC
    CTTCACAACT GCAGGATAGT GCATCTGGAC CTAAAGCCTG AGAACATCCT CGTGGATCAG
    AGCTCAGCCA AGCCGACCAT CAAGCTGGCT GACTTCGGAG ATGCCGTCCA ACTCAACACG
    ACCTACCACA TCCACCCATT GCTGGGGAAC CCCGAGTTCG CAGCCCCCGA GATCATCCTG
    GGGAACCCGG TCTCCCTGAC CTCAGACACG TGGAGCGTGG GCGTGCTGGC CTACGTGCTT
    CTCAGCGGCG TGTCCCCCTT CCTGGACGAC AGCGTGGAAG AGACCTGCCT CAACATTTGC
    CGCCTGGACT TCAGCTTCCC AGAGGACTAC TTCCAAGGAG TGAGCCAGAG GGCCAAGGAC
    TTCGTGTGCT TCCTACTGCA CGAGGACCCC GCCAAGCGCC CCTCGGCTGC GTCGGCCCTC
    CAGGAGCGGT GGCTGCAGGC GGGCCACGGC CACGGCAAAG GCGCGGGCGT CCTCGACACG
    TCCAGACTGA CCTCCTTCAT TGAGCGGCGC AAACACCAGA ACGACGTTCG ACCTATCCGT
    AGCATGAAAA ACTTCTTACA GAGCAGGCTT CTGCCTCGAG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837108.1
    CDS34..9198
    Translation

    Target ORF information:

    RefSeq Version XM_024981340.1
    Organism Bos taurus(cattle)
    Definition Bos taurus trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981340.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGTCTCCTA CATTGGCAGG GTTTCGAAAA AACGATGAAA TGAAAGCTAT GGATGTTTTG 
    CCCATTTTAA AGGAAAAAGT TGCATACCTT TCAGGTGGCA GAGACAAGCG CGGGGGTCCC
    ATCCTCACCT TCCCGGCCCG CAGCAACCAT GACAGAATAC GGCAGGAGGA CCTCCGGAGG
    CTCATCTCCT ACCTGGCCTG TATCCCCAGT GAAGAAGTCT GCAAGCGAGG GTTCACGGTG
    ATTGTGGACA TGCGTGGGTC CAAGTGGGAC TCCATCAAGC CGCTGCTGAA GATCTTACAA
    GAGTCCTTCC CCTGCTGCAT CCATGTCGCG CTGATAATCA AGCCAGACAA CTTTTGGCAG
    AAACAGAGGA CTAATTTTGG CAGTTCTAAA TTTGAATTTG AGACAAATAT GGTCTCTTTA
    GAAGGCCTTA CCAAAGTAGT TGATCCTTCT CAGCTAACGC CCGAGTTTGA CGGCTGCTTG
    GAGTACAACC ACGAAGAATG GATTGAGATC CGACTTGCTT TTGAAGACTA CATTAGCAAC
    GCGACCCACA TGCTCTCTCG GCTGGAGGAG CTCCAGGACA TCCTCGCCAA GAAGGAGTTG
    CCCCAGGATT TGGAGGGGGC GCGGAACATG ATCGAGGAGC ATTCCCAGCT GAAGAAGAAG
    GTGATTAAGG CCCCCATAGA GGACCTGGAC CTAGAGGGAC AGAAGCTGCT GCAGAGGATT
    CAGAGCAGCG AGAGCTTTCC CAAAAAGAAC TCGGGCTCAG GCAACGCAGA CCTGCAGAGC
    CTCTTACCCA AGGTGTCTGC CATGCTGGAC CGGCTGCACT CGACGCGGCA GCATCTGCAT
    CAGATGTGGC ACGTGCGCAA GCTGAAGCTG GACCAGTGCT TTCAGCTGAG GCTGTTCGAG
    CAGGATGCTG AGAAGATGTT TGACTGGATC ACACACAACA AGGGCCTGTT TCTGAACAGC
    TACACTGAGA TTGGGACCAG CCACCCTCAT GCCATGGAGC TCCAGACCCA GCACAACCAC
    TTTGCCATGA ACTGCATGAA CGTGTATGTG AACATCAACC GCATCATGTC GGTGGCCAAC
    CGCCTGGTGG AGTCGGGCCA CTATGCCTCC CAGCAGATCA AGCAGATCGC CAACCAGCTG
    GAGCAGGAGT GGAAGGCCTT CGCGGCTGCC CTGGACGAGC GTAGCACCTT GCTGGACATG
    TCATCTGTCT TCCACCAGAA GGCTGAGAAG TATATGAGCA ATGTGGACTC GTGGTGCAAG
    GCCTGCGGCG AGGTGGACCT GCCCTCGGAG CTTCAGGACC TGGAGGACGC TATTCACCAC
    CACCAGGGCG TGTACGAGCA CGTCACTCTT GCCTACTCCG AGGTGAGCCA GGACGGGAAG
    TCCCTGCTCG ACAAGCTCCA GCGGCCCCTG ACGCCCGGCA GCTCTGACTC GCTCACGGCC
    TCGGCCAACT ACTCGAAGGC CGTGCACCAC GTCCTGGACG TCATCCATGA GGTGCTGCAC
    CACCAGCGGC AGCTGGAGAA CATCTGGCAG CACCGCAAGG TGCGGCTCCA CCAGCGGCTG
    CAGCTGTGCG TCTTCCAGCA GGACGTCCAG CAGGTGCTGG ACTGGATCGA GAACCATGGA
    GAAGCATTTC TGAGCAAACA CACAGGTGTA GGGAAATCGC TCCATCGGGC CAGGGCTTTG
    CAGAAACGTC ATGAGGATTT TGAAGAAGTA GCACAGAACA CGTACACCAA TGCAGATAAG
    TTACTTGAGG CAGCCGAGCA GCTGGCCCAG ACGGGAGAAT GTGACCCGGA AGAGATCTAT
    CAGGCTGCCC ATCAGCTCGA GGACCGGATA CAGGATTTTG TGCGGCGCGT GGAGCAGCGG
    AAGATCCTCC TGGACATGTC GGTGTCTTTT CACACGCATG TGAAAGAGCT GTGGACATGG
    CTGGAGGAGC TGCAGAAGGA GCTGCTGGAC GACGTGTACG CCGAGTCCGT GGAGGCCGTG
    CAGGACCTCA GCAAGCGCTT CGGCCAGCAG CAGCAGACCA CCCTGCAGGT GACTGTGAAC
    GTCATCAAGG AAGGCGAGGA CCTGATCCAG CAGCTGAGGG ACTCAGCCAT CTCCAGCAAC
    AAGACGCCGC ACAACAGCTC CATCAGCCAC ATCGAAACGG TGCTGCAGCA GCTGGACGAG
    GCCCAGGCGC AGATGGAGGA GCTCTTCCAG GAGCGCAAGA TCAAGCTGGA GCTCTTCCTG
    CAGCTGCGCA TCTTCGAGCG GGACGCCATC GATATCATCT CAGACCTGGA GTCTTGGAAC
    GATGAGCTTT CTCAGCAGAT GAACGACTTC GACACGGAGG ACCTCACGAT CGCGGAGCAG
    CGCCTGCAGC ACCACGCGGA CAAGGCCCTG ACCATGAACA ACCTGACCTT CGACGTCATC
    CACCAGGGGC AGGACCTGCT GCAGTACGTC AACGAGGTGC AGGCCTCCGG TGTGGAGCTG
    CTTTGCGACA GAGATGTGGA CATGGCCACG CGGGTCCAGG ACCTCCTGGA GTTTCTGCAC
    GAGAAGCAGC AGGAACTGGA CCTCGCAGCT GAGCAGCACC GGAAGCACCT GGAGCAGTGC
    GTGCAGCTGC GCCACCTGCA GGCCGAGGTC AAGCAGGTGC TGGGCTGGAT CCGCAATGGA
    GAGTCCATGC TGAACGCCGG GCTCATCGCG GCCAGCTCGC TGCAGGAGGC AGAGCAGCTG
    CAGAGGGAGC ACGAGCAGTT CCAGCACGCC ATCGAGAAGA CACACCAGAG CGCCCTGCAG
    GTCCAGCAGA AGGCCGAGGC CATGCTGCAG GCCAATCACT ACGACATGGA CATGATCCGG
    GACTGTGCCG AGAAGGTCGC CTCACACTGG CAGCAGCTCA TGCTCAAGAT GGAAGACCGG
    CTGAAGCTGG TGAACGCGTC CGTGGCCTTC TACAAGACCT CAGAGCAGGT CTGCAGCGTC
    CTGGAGAGCC TGGAGCAGGA GTACAAGAGG GAAGAGGACT GGTGCGGAGG GGCCGACAAG
    CTGGGCCCCA ACTCCGAGAC GGACCACGTC ACCCCCATGA TCAGCAAGCA CCTCGAACAG
    AAGGAAGCAT TCCTGAAGGC TTGCACGCTT GCCAGGAGGA ACGCGGACGT CTTCCTGAAG
    TACCTGCACA GGAACAGCGT GAACATGCCA GGCATGGTGA CGCACATCAA GGCCCCCGAG
    CAGCAAGTGA AAAATATCTT GAACGAGCTC TTCCAGCGGG AGAACAGGGT GCTGCACTAC
    TGGACCATGA GGAAGCGGCG GCTGGACCAG TGCCAGCAGT TCGTGGTCTT CGAGCGAAGC
    GCCAAGCAGG CTCTGGAGTG GATCCACGAC AGCGGGGAGT TCTACCTCTC CACCCACACG
    TCCACGGGCT CAAGCATCCA GCACACCCAG GAGCTGCTGA AGGAGCATGA GGACTTCCAG
    ATCACCGCCA AGCAAACCAA AGAGAGAGTG AAGCTATTGA TACAGCTGGC CGACGGCTTT
    TGTGAAAAGG GGCATGCCCA TGCGGCAGAA ATAAAAAAAT GTGTCACTGC CGTGGATAAG
    AGGTACAGAG ACTTCTCTCT GCGGATGGAG AAATACAGGA CCTCTTTGGA GAAAGCCCTA
    GGGATTTCTT CAGATTCCAA CAAATCGAGC AAAAGTCTTC AGCTGGATAT TGTTCCGGCC
    AGTATCCCCG GCTCAGAGGT GAAACTCCGA GACGCCGCCC ATGAACTTAA TGAGGAAAAG
    CGGAAATCTG CCCGCAGGAA AGAGTTCATA ATGGCTGAGC TAATTCAAAC TGAAAAGGCT
    TACGTAAGAG ACCTCCGGGA GTGTATGGAT ACCTACCTGT GGGAAATGAC CAGCGGCGTG
    GAGGAGATCC CCCCGGGCAT TGTAAACAAA GAGCTCATCA TCTTCGGGAA CATGCAGGAA
    ATCTACGAGT TTCACAATAA CATATTCCTA AAAGAGCTGG AAAAATATGA GCAGTTGCCG
    GAGGACGTGG GACATTGCTT TGTAACCTGG GCAGACAAGT TTCAGATGTA TGTCACATAT
    TGCAAAAATA AGCCTGATTC TACTCAGCTG ATATTAGAGC ATGCAGGATC CTACTTTGAT
    GAAATACAGC AGCGGCACGG ATTAGCCAAT TCCATCTCCT CCTACCTTAT TAAACCAGTT
    CAGCGAATAA CGAAATATCA GCTCCTTTTA AAAGAGCTGC TCACGTGCTG CGAGGAGGGG
    AAGGGGGAGA TCAAGGATGG GCTGGAGGTG ATGCTCAGCG TGCCTAAGCG GGCCAACGAT
    GCCATGCACC TCAGCATGCT GGAAGGGTTC GATGAGAACA TTGAGTCTCA GGGAGAGCTG
    ATCCTGCAGG AATCCTTCCA AGTGTGGGAC CCCAAAACCT TAATCCGAAA GGGCCGTGAG
    CGGCACCTTT TCCTGTTTGA GATGTCCTTG GTGTTTAGTA AAGAAGTAAA AGATTCCAGT
    GGCAGAAGCA AGTACCTTTA TAAAAGCAAA TTGTTTACCT CAGAGCTGGG TGTCACAGAG
    CACGTTGAAG GAGACCCTTG CAAATTCGCA CTGTGGGTGG GGAGGACACC AACTTCAGAT
    AATAAGATTG TCCTTAAGGC CTCCAGTATT GAGAACAAGC AGGACTGGAT CAAGCATATC
    CGGGAGGTGA TCCAGGAAAG GACCATCCAC CTGAAAGGCG CCCTGAAGGA GCCCATCCAC
    ATCCCCAAAA CAGCTCCAGC CACGAGACAG AAGGGAAGGA GGGATGGAGA GGATTTGGAC
    AGCCAAGGAG ATGGCAGCAG CCAGCCCGAT ACGATTTCCA TTGCCTCCCG GACGTCTCAG
    AACACGCTGG ACAGTGACAA GCTGTCCGGC GGCTGCGAGC TGACCGTGGT GATCCACGAC
    TTCACGGCCT GCAACAGCAC CGAGCTCACC ATCCGCCGCG GCCAGACCGT GGAAGTCTTG
    GAGCGGCCCC ATGACAAACC CGACTGGTGC CTGGTGCGGA CCACTGACCG GTCGCCTGCG
    GCCGAGGGCC TGGTCCCCTG TGGCTCCCTG TGCATCGCCC ACTCCAGGAG CAGCATGGAG
    ATGGAGGGCA TCTTCAACCA CAAAGACTCA CTGTCCGTCT CCAGCAACGA CGCCAGCCCG
    CCCGCCTCCG TGGCCTCCCT CCAGCCCCAC ATGGTAGGGG CGCAGAGCTC GCCGGGCCCC
    AAGCGCCCGG GCAACACGCT GCGTAAGTGG CTGACAAGCC CCGTGCGGCG GCTGAGCAGC
    GGCAAGGCGG ATGGGCACGT GAAGAAGCTG GCGCACAAGC ACAAGAAGAG CCGCGAGGTC
    CGCAAGAGCG CCGACGCCGG CTCGCAGAAG GACTCGGACG ACAGCGCGGC CACCCCGCAG
    GACGAGACCG TGGAAGAGAG AGGCCGGAAC GAGGGCCTGA GCAGTGGCAC GCTTTCCAAG
    TCCTCATCTT CGGGCATGCA GAGCTGCGGA GAGGAAGAAG GGGAGGAGGG GGCCGACGCC
    GTGCCCCTGC CGCCACCCAT GGCCATCCAG CAGCACAGCC TGCTCCAGCC AGACTCCCAG
    GACGACAAGG CCTCTTCTCG GTTATTAGTC CGCCCCACCA GCTCCGAAAC ACCGAGCGCA
    GCCGAGCTTG TCAGTGCAAT CGAGGAACTC GTGAAAAGCA AGATGGCTCT GGAGGATCGT
    CCCAGCTCGC TCCTCGTCGA CCAGGGGGAC AGCAGCAGCC CCTCCTTCAA CCCTTCAGAC
    AACTCCCTGC TGTCCTCCTC CTCACCCATC GATGAGATGG AAGAGAGGAA GTCCAGCTCT
    TTAAAGAGAC GCCACTACGT TTTACAAGAA TTAGTGGAGA CAGAGCGTGA CTATGTGCGG
    GACCTCGGCT GTGTGGTGGA GGGCTACATG GCACTTATGA AAGAAGATGG TGTTCCTGAC
    GACATGAAAG GCAAAGACAA GATTGTGTTT GGCAACATCC ATCAGATTTA CGACTGGCAC
    AGAGACTTTT TTTTAGGAGA GTTGGAGAAG TGCCTTGAAG ATCCGGAAAA ACTAGGATCC
    CTTTTTGTTA AACATGAGAG AAGGTTGCAC ATGTACATAG TTTATTGCCA AAATAAACCA
    AAGTCTGAAC ACATTGTCTC AGAATACATT GACACCTTTT TTGAGGACTT AAAGCAGCGC
    CTGGGCCACA GGCTGCAGCT CACGGACTTG CTGATCAAAC CCGTTCAGAG GATCACGAAG
    TACCAGCTGC TGCTGAAGGA CTTCCTCAAG TATTCCAAAA AGGCCAGCCT GGATACATCA
    GAACTAGAGA GAGCCGTGGA GGTCATGTGC GTCGTCCCGA AGCGCTGCAA CGACATGATG
    AACGTCGGGA GGCTACAAGG CTTCGACGGT AAGATCGTGG CCCAGGGCAA GCTGCTCCTG
    CAGGACACGT TCTTGGTCAC GGACCAGGAC ACGGGGCTGC TGCCTCGCTG CAAAGAGAGG
    CGGGTGTTCC TCTTCGAGCA GATCGTCATT TTCAGTGAGC CGCTGGATAA AAAAAAGGGC
    TTCTCCATGC CAGGATTCCT GTTCAAGAAC AGTATCAAGG TGAGCTGCCT TTGCCTGGAG
    GAAAACGTGG AAAATGATCC CTGTAAGTTC GCTCTGACAT CGCGGACGGG TGACGTGGTG
    GAGACTTTCA TCCTGCACTC ATCCAGTCCA AGTGTCCGGC AGACGTGGAT CCATGAAATC
    AACCAGATTT TAGAAAACCA GCGCAATTTT TTAAACGCCT TGACATCACC CATCGAATAT
    CAGAGGAACC ACAGCGGGGG CGGCGGCAGC GGCGGGGGCG GGGGCAGCAG CGGGGCCCCC
    AGCGGCGGGG GCGGCGGCAG CAACCACAGC GGCGGTGGGG GCGGCCCCGG TGGCGGCGGC
    AGCGGCAGCT CGAGCAGAAG TAGACCCTCC CGGATTCCCC AGCCCGTCAG ACACCACTCC
    CCCGTGCTGG TCTCCTCTGC AGCCTCGAGC CAGGCAGAGG CAGACAAGAT GTCAGGTACG
    TGCACCCCGG GCCCGCCACC GCCTCCTCCC AGCCGCAGCC CCGCCCCGGA GGCCAGCGCC
    GGCCCGCCTG GCAGGGCGCC CCCCAGCAGC GAGCCCGACG GTCCCGAGCG AGACACCGAG
    CCGATCCCCA AGATGAAGGT GCTGGAGAGC CCCCGGAAGT CGTCCGGGGG CGTGCTGCCA
    CAGGACGGAG CCGCCAAGGA GGCACCGCGG AGCGGCCCGG GCCCCCTTCC GCTGGGCAAG
    GCCAGGCCCG GGGCCCCCTC GCCCCTGCAC TCACCTCTGG CCGCCGCCGC CGCGTCCCCC
    TCTCCGTTCG GCAAGGAGCC CTTGCCCCCC AGCAGCCCCC TGCAGAAGGG GGGCTCCTTC
    TGGAGCTCCG TGCCTGCCTC CCCTGCCAGC CGGCCCGGCT CCTTCACCTT CCCGGGGGAC
    AGCGACTCCC TGCAGCGGCA GGCACGGCGC CACGCGGCCC CTGGCAAGGA TGCGGACCGC
    ATGAGCACGT GCTCCTCGGC CAGCGAGCAG TCCGTGCAGT CCACCCAGAG CAACGGGAGT
    GAAAGCAGCA GCGGTAGCAA CATCTCCACC ATGTTGGTGA CACACGACTA CACGGCAGTG
    AAGGAGGATG AGATAAACGT CTATCAAGGA GAGGTCGTTC AAATTCTGGC CAGCAATCAA
    CAGAACATGT TTCTGGTGTT CCGAGCCGCC ACTGACCAGT GCCCCGCAGC CGAGGGCTGG
    ATTCCGGGCT TCGTCCTGGG GCACACCAGC GCAGTCATCA TGGAGAACCC TGACGGGACT
    CTCAAGAAGT CAACATCTTG GCACACAGCA CTCCGCTTAA GGAAAAAATC TGAGAAAAAA
    GATAAAGACG GCAAACGGGA AGGCAAGTTA GAGAACGGTT ACCGGAAGTC TCGGGAAGGA
    CTGAGCAACA AGGTATCTGT GAAGCTTCTC AATCCCAACT ACATATATGA CGTTCCCCCA
    GAATTCGTCA TTCCCTTGAG TGAGGTCACG TGTGAGGCAG GCGAGACCGT TGTTCTTCGA
    TGTCGGGTTT GTGGCCGCCC CAAGGCCTCC ATCACCTGGA AGGGCCCTGA ACACAACACC
    TTGAACAACG ACGGTCACTA CAGCATCTCC TACAGCGACT TGGGAGAGGC CGCTCTGAAG
    ATCGTGGGTG TGGCCGCCGA GGACGACGGC GTCTACACGT GCATCGCGGT GAACGACATG
    GGGTCAGCCT CGTCTTCAGC CAGCCTGAGG GTTCTAGGTC CAGGGAGTGA TGGCATCGTG
    GTGACCTGGA AGGACAACTT TGACTGCTTC TACAGCGAGG TGGCCGAGCT CGGCAGGGGC
    AGATTCTCTG TCGTGAAGAA ATGTGATCAG AAAGGCACCA AACGAGCCGT GGCCACTAAG
    TTTGTGAACA AGAAGCTGAT GAAGCGTGAC CAGGTCACCC ACGAACTTGG GATCCTGCAG
    AACCTCCAGC ACCCGCTGCT GGTGGGCCTC CTGGACACCT TCGAGACCCC CACCAGCTAC
    GTGCTGGTCC TGGAGATGGC CGACCAGGGA CGGCTCCTGG ACTGCGTGGT GCGGTGGGGG
    AACCTCACGG AGGGGAAGAT CCGGGCGTAC CTGGGGGAGG TTCTGGAAGC CGTGCGATAC
    CTTCACAACT GCAGGATAGT GCATCTGGAC CTAAAGCCTG AGAACATCCT CGTGGATCAG
    AGCTCAGCCA AGCCGACCAT CAAGCTGGCT GACTTCGGAG ATGCCGTCCA ACTCAACACG
    ACCTACCACA TCCACCCATT GCTGGGGAAC CCCGAGTTCG CAGCCCCCGA GATCATCCTG
    GGGAACCCGG TCTCCCTGAC CTCAGACACG TGGAGCGTGG GCGTGCTGGC CTACGTGCTT
    CTCAGCGGCG TGTCCCCCTT CCTGGACGAC AGCGTGGAAG AGACCTGCCT CAACATTTGC
    CGCCTGGACT TCAGCTTCCC AGAGGACTAC TTCCAAGGAG TGAGCCAGAG GGCCAAGGAC
    TTCGTGTGCT TCCTACTGCA CGAGGACCCC GCCAAGCGCC CCTCGGCTGC GTCGGCCCTC
    CAGGAGCGGT GGCTGCAGGC GGGCCACGGC CACGGCAAAG GCGCGGGCGT CCTCGACACG
    TCCAGACTGA CCTCCTTCAT TGAGCGGCGC AAACACCAGA ACGACGTTCG ACCTATCCGT
    AGCATGAAAA ACTTCTTACA GAGCAGGCTT CTGCCTCGAG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228049
    Clone ID Related Accession (Same CDS sequence) XM_024981341.1
    Accession Version XM_024981341.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product triple functional domain protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGGGTTTC GAAAAAACGA TGAAATGAAA GCTATGGATG TTTTGCCCAT TTTAAAGGAA 
    AAAGTTGCAT ACCTTTCAGG TGGCAGAGAC AAGCGCGGGG GTCCCATCCT CACCTTCCCG
    GCCCGCAGCA ACCATGACAG AATACGGCAG GAGGACCTCC GGAGGCTCAT CTCCTACCTG
    GCCTGTATCC CCAGTGAAGA AGTCTGCAAG CGAGGGTTCA CGGTGATTGT GGACATGCGT
    GGGTCCAAGT GGGACTCCAT CAAGCCGCTG CTGAAGATCT TACAAGAGTC CTTCCCCTGC
    TGCATCCATG TCGCGCTGAT AATCAAGCCA GACAACTTTT GGCAGAAACA GAGGACTAAT
    TTTGGCAGTT CTAAATTTGA ATTTGAGACA AATATGGTCT CTTTAGAAGG CCTTACCAAA
    GTAGTTGATC CTTCTCAGCT AACGCCCGAG TTTGACGGCT GCTTGGAGTA CAACCACGAA
    GAATGGATTG AGATCCGACT TGCTTTTGAA GACTACATTA GCAACGCGAC CCACATGCTC
    TCTCGGCTGG AGGAGCTCCA GGACATCCTC GCCAAGAAGG AGTTGCCCCA GGATTTGGAG
    GGGGCGCGGA ACATGATCGA GGAGCATTCC CAGCTGAAGA AGAAGGTGAT TAAGGCCCCC
    ATAGAGGACC TGGACCTAGA GGGACAGAAG CTGCTGCAGA GGATTCAGAG CAGCGAGAGC
    TTTCCCAAAA AGAACTCGGG CTCAGGCAAC GCAGACCTGC AGAGCCTCTT ACCCAAGGTG
    TCTGCCATGC TGGACCGGCT GCACTCGACG CGGCAGCATC TGCATCAGAT GTGGCACGTG
    CGCAAGCTGA AGCTGGACCA GTGCTTTCAG CTGAGGCTGT TCGAGCAGGA TGCTGAGAAG
    ATGTTTGACT GGATCACACA CAACAAGGGC CTGTTTCTGA ACAGCTACAC TGAGATTGGG
    ACCAGCCACC CTCATGCCAT GGAGCTCCAG ACCCAGCACA ACCACTTTGC CATGAACTGC
    ATGAACGTGT ATGTGAACAT CAACCGCATC ATGTCGGTGG CCAACCGCCT GGTGGAGTCG
    GGCCACTATG CCTCCCAGCA GATCAAGCAG ATCGCCAACC AGCTGGAGCA GGAGTGGAAG
    GCCTTCGCGG CTGCCCTGGA CGAGCGTAGC ACCTTGCTGG ACATGTCATC TGTCTTCCAC
    CAGAAGGCTG AGAAGTATAT GAGCAATGTG GACTCGTGGT GCAAGGCCTG CGGCGAGGTG
    GACCTGCCCT CGGAGCTTCA GGACCTGGAG GACGCTATTC ACCACCACCA GGGCGTGTAC
    GAGCACGTCA CTCTTGCCTA CTCCGAGGTG AGCCAGGACG GGAAGTCCCT GCTCGACAAG
    CTCCAGCGGC CCCTGACGCC CGGCAGCTCT GACTCGCTCA CGGCCTCGGC CAACTACTCG
    AAGGCCGTGC ACCACGTCCT GGACGTCATC CATGAGGTGC TGCACCACCA GCGGCAGCTG
    GAGAACATCT GGCAGCACCG CAAGGTGCGG CTCCACCAGC GGCTGCAGCT GTGCGTCTTC
    CAGCAGGACG TCCAGCAGGT GCTGGACTGG ATCGAGAACC ATGGAGAAGC ATTTCTGAGC
    AAACACACAG GTGTAGGGAA ATCGCTCCAT CGGGCCAGGG CTTTGCAGAA ACGTCATGAG
    GATTTTGAAG AAGTAGCACA GAACACGTAC ACCAATGCAG ATAAGTTACT TGAGGCAGCC
    GAGCAGCTGG CCCAGACGGG AGAATGTGAC CCGGAAGAGA TCTATCAGGC TGCCCATCAG
    CTCGAGGACC GGATACAGGA TTTTGTGCGG CGCGTGGAGC AGCGGAAGAT CCTCCTGGAC
    ATGTCGGTGT CTTTTCACAC GCATGTGAAA GAGCTGTGGA CATGGCTGGA GGAGCTGCAG
    AAGGAGCTGC TGGACGACGT GTACGCCGAG TCCGTGGAGG CCGTGCAGGA CCTCAGCAAG
    CGCTTCGGCC AGCAGCAGCA GACCACCCTG CAGGTGACTG TGAACGTCAT CAAGGAAGGC
    GAGGACCTGA TCCAGCAGCT GAGGGACTCA GCCATCTCCA GCAACAAGAC GCCGCACAAC
    AGCTCCATCA GCCACATCGA AACGGTGCTG CAGCAGCTGG ACGAGGCCCA GGCGCAGATG
    GAGGAGCTCT TCCAGGAGCG CAAGATCAAG CTGGAGCTCT TCCTGCAGCT GCGCATCTTC
    GAGCGGGACG CCATCGATAT CATCTCAGAC CTGGAGTCTT GGAACGATGA GCTTTCTCAG
    CAGATGAACG ACTTCGACAC GGAGGACCTC ACGATCGCGG AGCAGCGCCT GCAGCACCAC
    GCGGACAAGG CCCTGACCAT GAACAACCTG ACCTTCGACG TCATCCACCA GGGGCAGGAC
    CTGCTGCAGT ACGTCAACGA GGTGCAGGCC TCCGGTGTGG AGCTGCTTTG CGACAGAGAT
    GTGGACATGG CCACGCGGGT CCAGGACCTC CTGGAGTTTC TGCACGAGAA GCAGCAGGAA
    CTGGACCTCG CAGCTGAGCA GCACCGGAAG CACCTGGAGC AGTGCGTGCA GCTGCGCCAC
    CTGCAGGCCG AGGTCAAGCA GGTGCTGGGC TGGATCCGCA ATGGAGAGTC CATGCTGAAC
    GCCGGGCTCA TCGCGGCCAG CTCGCTGCAG GAGGCAGAGC AGCTGCAGAG GGAGCACGAG
    CAGTTCCAGC ACGCCATCGA GAAGACACAC CAGAGCGCCC TGCAGGTCCA GCAGAAGGCC
    GAGGCCATGC TGCAGGCCAA TCACTACGAC ATGGACATGA TCCGGGACTG TGCCGAGAAG
    GTCGCCTCAC ACTGGCAGCA GCTCATGCTC AAGATGGAAG ACCGGCTGAA GCTGGTGAAC
    GCGTCCGTGG CCTTCTACAA GACCTCAGAG CAGGTCTGCA GCGTCCTGGA GAGCCTGGAG
    CAGGAGTACA AGAGGGAAGA GGACTGGTGC GGAGGGGCCG ACAAGCTGGG CCCCAACTCC
    GAGACGGACC ACGTCACCCC CATGATCAGC AAGCACCTCG AACAGAAGGA AGCATTCCTG
    AAGGCTTGCA CGCTTGCCAG GAGGAACGCG GACGTCTTCC TGAAGTACCT GCACAGGAAC
    AGCGTGAACA TGCCAGGCAT GGTGACGCAC ATCAAGGCCC CCGAGCAGCA AGTGAAAAAT
    ATCTTGAACG AGCTCTTCCA GCGGGAGAAC AGGGTGCTGC ACTACTGGAC CATGAGGAAG
    CGGCGGCTGG ACCAGTGCCA GCAGTTCGTG GTCTTCGAGC GAAGCGCCAA GCAGGCTCTG
    GAGTGGATCC ACGACAGCGG GGAGTTCTAC CTCTCCACCC ACACGTCCAC GGGCTCAAGC
    ATCCAGCACA CCCAGGAGCT GCTGAAGGAG CATGAGGACT TCCAGATCAC CGCCAAGCAA
    ACCAAAGAGA GAGTGAAGCT ATTGATACAG CTGGCCGACG GCTTTTGTGA AAAGGGGCAT
    GCCCATGCGG CAGAAATAAA AAAATGTGTC ACTGCCGTGG ATAAGAGGTA CAGAGACTTC
    TCTCTGCGGA TGGAGAAATA CAGGACCTCT TTGGAGAAAG CCCTAGGGAT TTCTTCAGAT
    TCCAACAAAT CGAGCAAAAG TCTTCAGCTG GATATTGTTC CGGCCAGTAT CCCCGGCTCA
    GAGGTGAAAC TCCGAGACGC CGCCCATGAA CTTAATGAGG AAAAGCGGAA ATCTGCCCGC
    AGGAAAGAGT TCATAATGGC TGAGCTAATT CAAACTGAAA AGGCTTACGT AAGAGACCTC
    CGGGAGTGTA TGGATACCTA CCTGTGGGAA ATGACCAGCG GCGTGGAGGA GATCCCCCCG
    GGCATTGTAA ACAAAGAGCT CATCATCTTC GGGAACATGC AGGAAATCTA CGAGTTTCAC
    AATAACATAT TCCTAAAAGA GCTGGAAAAA TATGAGCAGT TGCCGGAGGA CGTGGGACAT
    TGCTTTGTAA CCTGGGCAGA CAAGTTTCAG ATGTATGTCA CATATTGCAA AAATAAGCCT
    GATTCTACTC AGCTGATATT AGAGCATGCA GGATCCTACT TTGATGAAAT ACAGCAGCGG
    CACGGATTAG CCAATTCCAT CTCCTCCTAC CTTATTAAAC CAGTTCAGCG AATAACGAAA
    TATCAGCTCC TTTTAAAAGA GCTGCTCACG TGCTGCGAGG AGGGGAAGGG GGAGATCAAG
    GATGGGCTGG AGGTGATGCT CAGCGTGCCT AAGCGGGCCA ACGATGCCAT GCACCTCAGC
    ATGCTGGAAG GGTTCGATGA GAACATTGAG TCTCAGGGAG AGCTGATCCT GCAGGAATCC
    TTCCAAGTGT GGGACCCCAA AACCTTAATC CGAAAGGGCC GTGAGCGGCA CCTTTTCCTG
    TTTGAGATGT CCTTGGTGTT TAGTAAAGAA GTAAAAGATT CCAGTGGCAG AAGCAAGTAC
    CTTTATAAAA GCAAATTGTT TACCTCAGAG CTGGGTGTCA CAGAGCACGT TGAAGGAGAC
    CCTTGCAAAT TCGCACTGTG GGTGGGGAGG ACACCAACTT CAGATAATAA GATTGTCCTT
    AAGGCCTCCA GTATTGAGAA CAAGCAGGAC TGGATCAAGC ATATCCGGGA GGTGATCCAG
    GAAAGGACCA TCCACCTGAA AGGCGCCCTG AAGGAGCCCA TCCACATCCC CAAAACAGCT
    CCAGCCACGA GACAGAAGGG AAGGAGGGAT GGAGAGGATT TGGACAGCCA AGGAGATGGC
    AGCAGCCAGC CCGATACGAT TTCCATTGCC TCCCGGACGT CTCAGAACAC GCTGGACAGT
    GACAAGCTGT CCGGCGGCTG CGAGCTGACC GTGGTGATCC ACGACTTCAC GGCCTGCAAC
    AGCACCGAGC TCACCATCCG CCGCGGCCAG ACCGTGGAAG TCTTGGAGCG GCCCCATGAC
    AAACCCGACT GGTGCCTGGT GCGGACCACT GACCGGTCGC CTGCGGCCGA GGGCCTGGTC
    CCCTGTGGCT CCCTGTGCAT CGCCCACTCC AGGAGCAGCA TGGAGATGGA GGGCATCTTC
    AACCACAAAG ACTCACTGTC CGTCTCCAGC AACGACGCCA GCCCGCCCGC CTCCGTGGCC
    TCCCTCCAGC CCCACATGGT AGGGGCGCAG AGCTCGCCGG GCCCCAAGCG CCCGGGCAAC
    ACGCTGCGTA AGTGGCTGAC AAGCCCCGTG CGGCGGCTGA GCAGCGGCAA GGCGGATGGG
    CACGTGAAGA AGCTGGCGCA CAAGCACAAG AAGAGCCGCG AGGTCCGCAA GAGCGCCGAC
    GCCGGCTCGC AGAAGGACTC GGACGACAGC GCGGCCACCC CGCAGGACGA GACCGTGGAA
    GAGAGAGGCC GGAACGAGGG CCTGAGCAGT GGCACGCTTT CCAAGTCCTC ATCTTCGGGC
    ATGCAGAGCT GCGGAGAGGA AGAAGGGGAG GAGGGGGCCG ACGCCGTGCC CCTGCCGCCA
    CCCATGGCCA TCCAGCAGCA CAGCCTGCTC CAGCCAGACT CCCAGGACGA CAAGGCCTCT
    TCTCGGTTAT TAGTCCGCCC CACCAGCTCC GAAACACCGA GCGCAGCCGA GCTTGTCAGT
    GCAATCGAGG AACTCGTGAA AAGCAAGATG GCTCTGGAGG ATCGTCCCAG CTCGCTCCTC
    GTCGACCAGG GGGACAGCAG CAGCCCCTCC TTCAACCCTT CAGACAACTC CCTGCTGTCC
    TCCTCCTCAC CCATCGATGA GATGGAAGAG AGGAAGTCCA GCTCTTTAAA GAGACGCCAC
    TACGTTTTAC AAGAATTAGT GGAGACAGAG CGTGACTATG TGCGGGACCT CGGCTGTGTG
    GTGGAGGGCT ACATGGCACT TATGAAAGAA GATGGTGTTC CTGACGACAT GAAAGGCAAA
    GACAAGATTG TGTTTGGCAA CATCCATCAG ATTTACGACT GGCACAGAGA CTTTTTTTTA
    GGAGAGTTGG AGAAGTGCCT TGAAGATCCG GAAAAACTAG GATCCCTTTT TGTTAAACAT
    GAGAGAAGGT TGCACATGTA CATAGTTTAT TGCCAAAATA AACCAAAGTC TGAACACATT
    GTCTCAGAAT ACATTGACAC CTTTTTTGAG GACTTAAAGC AGCGCCTGGG CCACAGGCTG
    CAGCTCACGG ACTTGCTGAT CAAACCCGTT CAGAGGATCA CGAAGTACCA GCTGCTGCTG
    AAGGACTTCC TCAAGTATTC CAAAAAGGCC AGCCTGGATA CATCAGAACT AGAGAGAGCC
    GTGGAGGTCA TGTGCGTCGT CCCGAAGCGC TGCAACGACA TGATGAACGT CGGGAGGCTA
    CAAGGCTTCG ACGGTAAGAT CGTGGCCCAG GGCAAGCTGC TCCTGCAGGA CACGTTCTTG
    GTCACGGACC AGGACACGGG GCTGCTGCCT CGCTGCAAAG AGAGGCGGGT GTTCCTCTTC
    GAGCAGATCG TCATTTTCAG TGAGCCGCTG GATAAAAAAA AGGGCTTCTC CATGCCAGGA
    TTCCTGTTCA AGAACAGTAT CAAGGTGAGC TGCCTTTGCC TGGAGGAAAA CGTGGAAAAT
    GATCCCTGTA AGTTCGCTCT GACATCGCGG ACGGGTGACG TGGTGGAGAC TTTCATCCTG
    CACTCATCCA GTCCAAGTGT CCGGCAGACG TGGATCCATG AAATCAACCA GATTTTAGAA
    AACCAGCGCA ATTTTTTAAA CGCCTTGACA TCACCCATCG AATATCAGAG GAACCACAGC
    GGGGGCGGCG GCAGCGGCGG GGGCGGGGGC AGCAGCGGGG CCCCCAGCGG CGGGGGCGGC
    GGCAGCAACC ACAGCGGCGG TGGGGGCGGC CCCGGTGGCG GCGGCAGCGG CAGCTCGAGC
    AGAAGTAGAC CCTCCCGGAT TCCCCAGCCC GTCAGACACC ACTCCCCCGT GCTGGTCTCC
    TCTGCAGCCT CGAGCCAGGC AGAGGCAGAC AAGATGTCAG GTACGTGCAC CCCGGGCCCG
    CCACCGCCTC CTCCCAGCCG CAGCCCCGCC CCGGAGGCCA GCGCCGGCCC GCCTGGCAGG
    GCGCCCCCCA GCAGCGAGCC CGACGGTCCC GAGCGAGACA CCGAGCCGAT CCCCAAGATG
    AAGGTGCTGG AGAGCCCCCG GAAGTCGTCC GGGGGCGTGC TGCCACAGGA CGGAGCCGCC
    AAGGAGGCAC CGCGGAGCGG CCCGGGCCCC CTTCCGCTGG GCAAGGCCAG GCCCGGGGCC
    CCCTCGCCCC TGCACTCACC TCTGGCCGCC GCCGCCGCGT CCCCCTCTCC GTTCGGCAAG
    GAGCCCTTGC CCCCCAGCAG CCCCCTGCAG AAGGGGGGCT CCTTCTGGAG CTCCGTGCCT
    GCCTCCCCTG CCAGCCGGCC CGGCTCCTTC ACCTTCCCGG GGGACAGCGA CTCCCTGCAG
    CGGCAGGCAC GGCGCCACGC GGCCCCTGGC AAGGATGCGG ACCGCATGAG CACGTGCTCC
    TCGGCCAGCG AGCAGTCCGT GCAGTCCACC CAGAGCAACG GGAGTGAAAG CAGCAGCGGT
    AGCAACATCT CCACCATGTT GGTGACACAC GACTACACGG CAGTGAAGGA GGATGAGATA
    AACGTCTATC AAGGAGAGGT CGTTCAAATT CTGGCCAGCA ATCAACAGAA CATGTTTCTG
    GTGTTCCGAG CCGCCACTGA CCAGTGCCCC GCAGCCGAGG GCTGGATTCC GGGCTTCGTC
    CTGGGGCACA CCAGCGCAGT CATCATGGAG AACCCTGACG GGACTCTCAA GAAGTCAACA
    TCTTGGCACA CAGCACTCCG CTTAAGGAAA AAATCTGAGA AAAAAGATAA AGACGGCAAA
    CGGGAAGGCA AGTTAGAGAA CGGTTACCGG AAGTCTCGGG AAGGACTGAG CAACAAGGTA
    TCTGTGAAGC TTCTCAATCC CAACTACATA TATGACGTTC CCCCAGAATT CGTCATTCCC
    TTGAGTGAGG TCACGTGTGA GGCAGGCGAG ACCGTTGTTC TTCGATGTCG GGTTTGTGGC
    CGCCCCAAGG CCTCCATCAC CTGGAAGGGC CCTGAACACA ACACCTTGAA CAACGACGGT
    CACTACAGCA TCTCCTACAG CGACTTGGGA GAGGCCGCTC TGAAGATCGT GGGTGTGGCC
    GCCGAGGACG ACGGCGTCTA CACGTGCATC GCGGTGAACG ACATGGGGTC AGCCTCGTCT
    TCAGCCAGCC TGAGGGTTCT AGGTCCAGGG AGTGATGGCA TCGTGGTGAC CTGGAAGGAC
    AACTTTGACT GCTTCTACAG CGAGGTGGCC GAGCTCGGCA GGGGCAGATT CTCTGTCGTG
    AAGAAATGTG ATCAGAAAGG CACCAAACGA GCCGTGGCCA CTAAGTTTGT GAACAAGAAG
    CTGATGAAGC GTGACCAGGT CACCCACGAA CTTGGGATCC TGCAGAACCT CCAGCACCCG
    CTGCTGGTGG GCCTCCTGGA CACCTTCGAG ACCCCCACCA GCTACGTGCT GGTCCTGGAG
    ATGGCCGACC AGGGACGGCT CCTGGACTGC GTGGTGCGGT GGGGGAACCT CACGGAGGGG
    AAGATCCGGG CGTACCTGGG GGAGGTTCTG GAAGCCGTGC GATACCTTCA CAACTGCAGG
    ATAGTGCATC TGGACCTAAA GCCTGAGAAC ATCCTCGTGG ATCAGAGCTC AGCCAAGCCG
    ACCATCAAGC TGGCTGACTT CGGAGATGCC GTCCAACTCA ACACGACCTA CCACATCCAC
    CCATTGCTGG GGAACCCCGA GTTCGCAGCC CCCGAGATCA TCCTGGGGAA CCCGGTCTCC
    CTGACCTCAG ACACGTGGAG CGTGGGCGTG CTGGCCTACG TGCTTCTCAG CGGCGTGTCC
    CCCTTCCTGG ACGACAGCGT GGAAGAGACC TGCCTCAACA TTTGCCGCCT GGACTTCAGC
    TTCCCAGAGG ACTACTTCCA AGGAGTGAGC CAGAGGGCCA AGGACTTCGT GTGCTTCCTA
    CTGCACGAGG ACCCCGCCAA GCGCCCCTCG GCTGCGTCGG CCCTCCAGGA GCGGTGGCTG
    CAGGCGGGCC ACGGCCACGG CAAAGGCGCG GGCGTCCTCG ACACGTCCAG ACTGACCTCC
    TTCATTGAGC GGCGCAAACA CCAGAACGAC GTTCGACCTA TCCGTAGCAT GAAAAACTTC
    TTACAGAGCA GGCTTCTGCC TCGAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837109.1
    CDS22..9171
    Translation

    Target ORF information:

    RefSeq Version XM_024981341.1
    Organism Bos taurus(cattle)
    Definition Bos taurus trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981341.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGGGTTTC GAAAAAACGA TGAAATGAAA GCTATGGATG TTTTGCCCAT TTTAAAGGAA 
    AAAGTTGCAT ACCTTTCAGG TGGCAGAGAC AAGCGCGGGG GTCCCATCCT CACCTTCCCG
    GCCCGCAGCA ACCATGACAG AATACGGCAG GAGGACCTCC GGAGGCTCAT CTCCTACCTG
    GCCTGTATCC CCAGTGAAGA AGTCTGCAAG CGAGGGTTCA CGGTGATTGT GGACATGCGT
    GGGTCCAAGT GGGACTCCAT CAAGCCGCTG CTGAAGATCT TACAAGAGTC CTTCCCCTGC
    TGCATCCATG TCGCGCTGAT AATCAAGCCA GACAACTTTT GGCAGAAACA GAGGACTAAT
    TTTGGCAGTT CTAAATTTGA ATTTGAGACA AATATGGTCT CTTTAGAAGG CCTTACCAAA
    GTAGTTGATC CTTCTCAGCT AACGCCCGAG TTTGACGGCT GCTTGGAGTA CAACCACGAA
    GAATGGATTG AGATCCGACT TGCTTTTGAA GACTACATTA GCAACGCGAC CCACATGCTC
    TCTCGGCTGG AGGAGCTCCA GGACATCCTC GCCAAGAAGG AGTTGCCCCA GGATTTGGAG
    GGGGCGCGGA ACATGATCGA GGAGCATTCC CAGCTGAAGA AGAAGGTGAT TAAGGCCCCC
    ATAGAGGACC TGGACCTAGA GGGACAGAAG CTGCTGCAGA GGATTCAGAG CAGCGAGAGC
    TTTCCCAAAA AGAACTCGGG CTCAGGCAAC GCAGACCTGC AGAGCCTCTT ACCCAAGGTG
    TCTGCCATGC TGGACCGGCT GCACTCGACG CGGCAGCATC TGCATCAGAT GTGGCACGTG
    CGCAAGCTGA AGCTGGACCA GTGCTTTCAG CTGAGGCTGT TCGAGCAGGA TGCTGAGAAG
    ATGTTTGACT GGATCACACA CAACAAGGGC CTGTTTCTGA ACAGCTACAC TGAGATTGGG
    ACCAGCCACC CTCATGCCAT GGAGCTCCAG ACCCAGCACA ACCACTTTGC CATGAACTGC
    ATGAACGTGT ATGTGAACAT CAACCGCATC ATGTCGGTGG CCAACCGCCT GGTGGAGTCG
    GGCCACTATG CCTCCCAGCA GATCAAGCAG ATCGCCAACC AGCTGGAGCA GGAGTGGAAG
    GCCTTCGCGG CTGCCCTGGA CGAGCGTAGC ACCTTGCTGG ACATGTCATC TGTCTTCCAC
    CAGAAGGCTG AGAAGTATAT GAGCAATGTG GACTCGTGGT GCAAGGCCTG CGGCGAGGTG
    GACCTGCCCT CGGAGCTTCA GGACCTGGAG GACGCTATTC ACCACCACCA GGGCGTGTAC
    GAGCACGTCA CTCTTGCCTA CTCCGAGGTG AGCCAGGACG GGAAGTCCCT GCTCGACAAG
    CTCCAGCGGC CCCTGACGCC CGGCAGCTCT GACTCGCTCA CGGCCTCGGC CAACTACTCG
    AAGGCCGTGC ACCACGTCCT GGACGTCATC CATGAGGTGC TGCACCACCA GCGGCAGCTG
    GAGAACATCT GGCAGCACCG CAAGGTGCGG CTCCACCAGC GGCTGCAGCT GTGCGTCTTC
    CAGCAGGACG TCCAGCAGGT GCTGGACTGG ATCGAGAACC ATGGAGAAGC ATTTCTGAGC
    AAACACACAG GTGTAGGGAA ATCGCTCCAT CGGGCCAGGG CTTTGCAGAA ACGTCATGAG
    GATTTTGAAG AAGTAGCACA GAACACGTAC ACCAATGCAG ATAAGTTACT TGAGGCAGCC
    GAGCAGCTGG CCCAGACGGG AGAATGTGAC CCGGAAGAGA TCTATCAGGC TGCCCATCAG
    CTCGAGGACC GGATACAGGA TTTTGTGCGG CGCGTGGAGC AGCGGAAGAT CCTCCTGGAC
    ATGTCGGTGT CTTTTCACAC GCATGTGAAA GAGCTGTGGA CATGGCTGGA GGAGCTGCAG
    AAGGAGCTGC TGGACGACGT GTACGCCGAG TCCGTGGAGG CCGTGCAGGA CCTCAGCAAG
    CGCTTCGGCC AGCAGCAGCA GACCACCCTG CAGGTGACTG TGAACGTCAT CAAGGAAGGC
    GAGGACCTGA TCCAGCAGCT GAGGGACTCA GCCATCTCCA GCAACAAGAC GCCGCACAAC
    AGCTCCATCA GCCACATCGA AACGGTGCTG CAGCAGCTGG ACGAGGCCCA GGCGCAGATG
    GAGGAGCTCT TCCAGGAGCG CAAGATCAAG CTGGAGCTCT TCCTGCAGCT GCGCATCTTC
    GAGCGGGACG CCATCGATAT CATCTCAGAC CTGGAGTCTT GGAACGATGA GCTTTCTCAG
    CAGATGAACG ACTTCGACAC GGAGGACCTC ACGATCGCGG AGCAGCGCCT GCAGCACCAC
    GCGGACAAGG CCCTGACCAT GAACAACCTG ACCTTCGACG TCATCCACCA GGGGCAGGAC
    CTGCTGCAGT ACGTCAACGA GGTGCAGGCC TCCGGTGTGG AGCTGCTTTG CGACAGAGAT
    GTGGACATGG CCACGCGGGT CCAGGACCTC CTGGAGTTTC TGCACGAGAA GCAGCAGGAA
    CTGGACCTCG CAGCTGAGCA GCACCGGAAG CACCTGGAGC AGTGCGTGCA GCTGCGCCAC
    CTGCAGGCCG AGGTCAAGCA GGTGCTGGGC TGGATCCGCA ATGGAGAGTC CATGCTGAAC
    GCCGGGCTCA TCGCGGCCAG CTCGCTGCAG GAGGCAGAGC AGCTGCAGAG GGAGCACGAG
    CAGTTCCAGC ACGCCATCGA GAAGACACAC CAGAGCGCCC TGCAGGTCCA GCAGAAGGCC
    GAGGCCATGC TGCAGGCCAA TCACTACGAC ATGGACATGA TCCGGGACTG TGCCGAGAAG
    GTCGCCTCAC ACTGGCAGCA GCTCATGCTC AAGATGGAAG ACCGGCTGAA GCTGGTGAAC
    GCGTCCGTGG CCTTCTACAA GACCTCAGAG CAGGTCTGCA GCGTCCTGGA GAGCCTGGAG
    CAGGAGTACA AGAGGGAAGA GGACTGGTGC GGAGGGGCCG ACAAGCTGGG CCCCAACTCC
    GAGACGGACC ACGTCACCCC CATGATCAGC AAGCACCTCG AACAGAAGGA AGCATTCCTG
    AAGGCTTGCA CGCTTGCCAG GAGGAACGCG GACGTCTTCC TGAAGTACCT GCACAGGAAC
    AGCGTGAACA TGCCAGGCAT GGTGACGCAC ATCAAGGCCC CCGAGCAGCA AGTGAAAAAT
    ATCTTGAACG AGCTCTTCCA GCGGGAGAAC AGGGTGCTGC ACTACTGGAC CATGAGGAAG
    CGGCGGCTGG ACCAGTGCCA GCAGTTCGTG GTCTTCGAGC GAAGCGCCAA GCAGGCTCTG
    GAGTGGATCC ACGACAGCGG GGAGTTCTAC CTCTCCACCC ACACGTCCAC GGGCTCAAGC
    ATCCAGCACA CCCAGGAGCT GCTGAAGGAG CATGAGGACT TCCAGATCAC CGCCAAGCAA
    ACCAAAGAGA GAGTGAAGCT ATTGATACAG CTGGCCGACG GCTTTTGTGA AAAGGGGCAT
    GCCCATGCGG CAGAAATAAA AAAATGTGTC ACTGCCGTGG ATAAGAGGTA CAGAGACTTC
    TCTCTGCGGA TGGAGAAATA CAGGACCTCT TTGGAGAAAG CCCTAGGGAT TTCTTCAGAT
    TCCAACAAAT CGAGCAAAAG TCTTCAGCTG GATATTGTTC CGGCCAGTAT CCCCGGCTCA
    GAGGTGAAAC TCCGAGACGC CGCCCATGAA CTTAATGAGG AAAAGCGGAA ATCTGCCCGC
    AGGAAAGAGT TCATAATGGC TGAGCTAATT CAAACTGAAA AGGCTTACGT AAGAGACCTC
    CGGGAGTGTA TGGATACCTA CCTGTGGGAA ATGACCAGCG GCGTGGAGGA GATCCCCCCG
    GGCATTGTAA ACAAAGAGCT CATCATCTTC GGGAACATGC AGGAAATCTA CGAGTTTCAC
    AATAACATAT TCCTAAAAGA GCTGGAAAAA TATGAGCAGT TGCCGGAGGA CGTGGGACAT
    TGCTTTGTAA CCTGGGCAGA CAAGTTTCAG ATGTATGTCA CATATTGCAA AAATAAGCCT
    GATTCTACTC AGCTGATATT AGAGCATGCA GGATCCTACT TTGATGAAAT ACAGCAGCGG
    CACGGATTAG CCAATTCCAT CTCCTCCTAC CTTATTAAAC CAGTTCAGCG AATAACGAAA
    TATCAGCTCC TTTTAAAAGA GCTGCTCACG TGCTGCGAGG AGGGGAAGGG GGAGATCAAG
    GATGGGCTGG AGGTGATGCT CAGCGTGCCT AAGCGGGCCA ACGATGCCAT GCACCTCAGC
    ATGCTGGAAG GGTTCGATGA GAACATTGAG TCTCAGGGAG AGCTGATCCT GCAGGAATCC
    TTCCAAGTGT GGGACCCCAA AACCTTAATC CGAAAGGGCC GTGAGCGGCA CCTTTTCCTG
    TTTGAGATGT CCTTGGTGTT TAGTAAAGAA GTAAAAGATT CCAGTGGCAG AAGCAAGTAC
    CTTTATAAAA GCAAATTGTT TACCTCAGAG CTGGGTGTCA CAGAGCACGT TGAAGGAGAC
    CCTTGCAAAT TCGCACTGTG GGTGGGGAGG ACACCAACTT CAGATAATAA GATTGTCCTT
    AAGGCCTCCA GTATTGAGAA CAAGCAGGAC TGGATCAAGC ATATCCGGGA GGTGATCCAG
    GAAAGGACCA TCCACCTGAA AGGCGCCCTG AAGGAGCCCA TCCACATCCC CAAAACAGCT
    CCAGCCACGA GACAGAAGGG AAGGAGGGAT GGAGAGGATT TGGACAGCCA AGGAGATGGC
    AGCAGCCAGC CCGATACGAT TTCCATTGCC TCCCGGACGT CTCAGAACAC GCTGGACAGT
    GACAAGCTGT CCGGCGGCTG CGAGCTGACC GTGGTGATCC ACGACTTCAC GGCCTGCAAC
    AGCACCGAGC TCACCATCCG CCGCGGCCAG ACCGTGGAAG TCTTGGAGCG GCCCCATGAC
    AAACCCGACT GGTGCCTGGT GCGGACCACT GACCGGTCGC CTGCGGCCGA GGGCCTGGTC
    CCCTGTGGCT CCCTGTGCAT CGCCCACTCC AGGAGCAGCA TGGAGATGGA GGGCATCTTC
    AACCACAAAG ACTCACTGTC CGTCTCCAGC AACGACGCCA GCCCGCCCGC CTCCGTGGCC
    TCCCTCCAGC CCCACATGGT AGGGGCGCAG AGCTCGCCGG GCCCCAAGCG CCCGGGCAAC
    ACGCTGCGTA AGTGGCTGAC AAGCCCCGTG CGGCGGCTGA GCAGCGGCAA GGCGGATGGG
    CACGTGAAGA AGCTGGCGCA CAAGCACAAG AAGAGCCGCG AGGTCCGCAA GAGCGCCGAC
    GCCGGCTCGC AGAAGGACTC GGACGACAGC GCGGCCACCC CGCAGGACGA GACCGTGGAA
    GAGAGAGGCC GGAACGAGGG CCTGAGCAGT GGCACGCTTT CCAAGTCCTC ATCTTCGGGC
    ATGCAGAGCT GCGGAGAGGA AGAAGGGGAG GAGGGGGCCG ACGCCGTGCC CCTGCCGCCA
    CCCATGGCCA TCCAGCAGCA CAGCCTGCTC CAGCCAGACT CCCAGGACGA CAAGGCCTCT
    TCTCGGTTAT TAGTCCGCCC CACCAGCTCC GAAACACCGA GCGCAGCCGA GCTTGTCAGT
    GCAATCGAGG AACTCGTGAA AAGCAAGATG GCTCTGGAGG ATCGTCCCAG CTCGCTCCTC
    GTCGACCAGG GGGACAGCAG CAGCCCCTCC TTCAACCCTT CAGACAACTC CCTGCTGTCC
    TCCTCCTCAC CCATCGATGA GATGGAAGAG AGGAAGTCCA GCTCTTTAAA GAGACGCCAC
    TACGTTTTAC AAGAATTAGT GGAGACAGAG CGTGACTATG TGCGGGACCT CGGCTGTGTG
    GTGGAGGGCT ACATGGCACT TATGAAAGAA GATGGTGTTC CTGACGACAT GAAAGGCAAA
    GACAAGATTG TGTTTGGCAA CATCCATCAG ATTTACGACT GGCACAGAGA CTTTTTTTTA
    GGAGAGTTGG AGAAGTGCCT TGAAGATCCG GAAAAACTAG GATCCCTTTT TGTTAAACAT
    GAGAGAAGGT TGCACATGTA CATAGTTTAT TGCCAAAATA AACCAAAGTC TGAACACATT
    GTCTCAGAAT ACATTGACAC CTTTTTTGAG GACTTAAAGC AGCGCCTGGG CCACAGGCTG
    CAGCTCACGG ACTTGCTGAT CAAACCCGTT CAGAGGATCA CGAAGTACCA GCTGCTGCTG
    AAGGACTTCC TCAAGTATTC CAAAAAGGCC AGCCTGGATA CATCAGAACT AGAGAGAGCC
    GTGGAGGTCA TGTGCGTCGT CCCGAAGCGC TGCAACGACA TGATGAACGT CGGGAGGCTA
    CAAGGCTTCG ACGGTAAGAT CGTGGCCCAG GGCAAGCTGC TCCTGCAGGA CACGTTCTTG
    GTCACGGACC AGGACACGGG GCTGCTGCCT CGCTGCAAAG AGAGGCGGGT GTTCCTCTTC
    GAGCAGATCG TCATTTTCAG TGAGCCGCTG GATAAAAAAA AGGGCTTCTC CATGCCAGGA
    TTCCTGTTCA AGAACAGTAT CAAGGTGAGC TGCCTTTGCC TGGAGGAAAA CGTGGAAAAT
    GATCCCTGTA AGTTCGCTCT GACATCGCGG ACGGGTGACG TGGTGGAGAC TTTCATCCTG
    CACTCATCCA GTCCAAGTGT CCGGCAGACG TGGATCCATG AAATCAACCA GATTTTAGAA
    AACCAGCGCA ATTTTTTAAA CGCCTTGACA TCACCCATCG AATATCAGAG GAACCACAGC
    GGGGGCGGCG GCAGCGGCGG GGGCGGGGGC AGCAGCGGGG CCCCCAGCGG CGGGGGCGGC
    GGCAGCAACC ACAGCGGCGG TGGGGGCGGC CCCGGTGGCG GCGGCAGCGG CAGCTCGAGC
    AGAAGTAGAC CCTCCCGGAT TCCCCAGCCC GTCAGACACC ACTCCCCCGT GCTGGTCTCC
    TCTGCAGCCT CGAGCCAGGC AGAGGCAGAC AAGATGTCAG GTACGTGCAC CCCGGGCCCG
    CCACCGCCTC CTCCCAGCCG CAGCCCCGCC CCGGAGGCCA GCGCCGGCCC GCCTGGCAGG
    GCGCCCCCCA GCAGCGAGCC CGACGGTCCC GAGCGAGACA CCGAGCCGAT CCCCAAGATG
    AAGGTGCTGG AGAGCCCCCG GAAGTCGTCC GGGGGCGTGC TGCCACAGGA CGGAGCCGCC
    AAGGAGGCAC CGCGGAGCGG CCCGGGCCCC CTTCCGCTGG GCAAGGCCAG GCCCGGGGCC
    CCCTCGCCCC TGCACTCACC TCTGGCCGCC GCCGCCGCGT CCCCCTCTCC GTTCGGCAAG
    GAGCCCTTGC CCCCCAGCAG CCCCCTGCAG AAGGGGGGCT CCTTCTGGAG CTCCGTGCCT
    GCCTCCCCTG CCAGCCGGCC CGGCTCCTTC ACCTTCCCGG GGGACAGCGA CTCCCTGCAG
    CGGCAGGCAC GGCGCCACGC GGCCCCTGGC AAGGATGCGG ACCGCATGAG CACGTGCTCC
    TCGGCCAGCG AGCAGTCCGT GCAGTCCACC CAGAGCAACG GGAGTGAAAG CAGCAGCGGT
    AGCAACATCT CCACCATGTT GGTGACACAC GACTACACGG CAGTGAAGGA GGATGAGATA
    AACGTCTATC AAGGAGAGGT CGTTCAAATT CTGGCCAGCA ATCAACAGAA CATGTTTCTG
    GTGTTCCGAG CCGCCACTGA CCAGTGCCCC GCAGCCGAGG GCTGGATTCC GGGCTTCGTC
    CTGGGGCACA CCAGCGCAGT CATCATGGAG AACCCTGACG GGACTCTCAA GAAGTCAACA
    TCTTGGCACA CAGCACTCCG CTTAAGGAAA AAATCTGAGA AAAAAGATAA AGACGGCAAA
    CGGGAAGGCA AGTTAGAGAA CGGTTACCGG AAGTCTCGGG AAGGACTGAG CAACAAGGTA
    TCTGTGAAGC TTCTCAATCC CAACTACATA TATGACGTTC CCCCAGAATT CGTCATTCCC
    TTGAGTGAGG TCACGTGTGA GGCAGGCGAG ACCGTTGTTC TTCGATGTCG GGTTTGTGGC
    CGCCCCAAGG CCTCCATCAC CTGGAAGGGC CCTGAACACA ACACCTTGAA CAACGACGGT
    CACTACAGCA TCTCCTACAG CGACTTGGGA GAGGCCGCTC TGAAGATCGT GGGTGTGGCC
    GCCGAGGACG ACGGCGTCTA CACGTGCATC GCGGTGAACG ACATGGGGTC AGCCTCGTCT
    TCAGCCAGCC TGAGGGTTCT AGGTCCAGGG AGTGATGGCA TCGTGGTGAC CTGGAAGGAC
    AACTTTGACT GCTTCTACAG CGAGGTGGCC GAGCTCGGCA GGGGCAGATT CTCTGTCGTG
    AAGAAATGTG ATCAGAAAGG CACCAAACGA GCCGTGGCCA CTAAGTTTGT GAACAAGAAG
    CTGATGAAGC GTGACCAGGT CACCCACGAA CTTGGGATCC TGCAGAACCT CCAGCACCCG
    CTGCTGGTGG GCCTCCTGGA CACCTTCGAG ACCCCCACCA GCTACGTGCT GGTCCTGGAG
    ATGGCCGACC AGGGACGGCT CCTGGACTGC GTGGTGCGGT GGGGGAACCT CACGGAGGGG
    AAGATCCGGG CGTACCTGGG GGAGGTTCTG GAAGCCGTGC GATACCTTCA CAACTGCAGG
    ATAGTGCATC TGGACCTAAA GCCTGAGAAC ATCCTCGTGG ATCAGAGCTC AGCCAAGCCG
    ACCATCAAGC TGGCTGACTT CGGAGATGCC GTCCAACTCA ACACGACCTA CCACATCCAC
    CCATTGCTGG GGAACCCCGA GTTCGCAGCC CCCGAGATCA TCCTGGGGAA CCCGGTCTCC
    CTGACCTCAG ACACGTGGAG CGTGGGCGTG CTGGCCTACG TGCTTCTCAG CGGCGTGTCC
    CCCTTCCTGG ACGACAGCGT GGAAGAGACC TGCCTCAACA TTTGCCGCCT GGACTTCAGC
    TTCCCAGAGG ACTACTTCCA AGGAGTGAGC CAGAGGGCCA AGGACTTCGT GTGCTTCCTA
    CTGCACGAGG ACCCCGCCAA GCGCCCCTCG GCTGCGTCGG CCCTCCAGGA GCGGTGGCTG
    CAGGCGGGCC ACGGCCACGG CAAAGGCGCG GGCGTCCTCG ACACGTCCAG ACTGACCTCC
    TTCATTGAGC GGCGCAAACA CCAGAACGAC GTTCGACCTA TCCGTAGCAT GAAAAACTTC
    TTACAGAGCA GGCTTCTGCC TCGAGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.