ATP1A4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP1A4
Entrez Gene ID	537960
Full Name	ATPase Na+/K+ transporting subunit alpha 4
General protein information	Preferred Names ATPase Na+/K+ transporting subunit alpha 4 Names sodium/potassium-transporting ATPase subunit alpha-4 ATPase, Na+/K+ transporting, alpha 4 polypeptide
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005203533.4	XP_005203590.1	sodium/potassium-transporting ATPase subunit alpha-4 isoform X1
NM_001144103.2	NP_001137575.2	sodium/potassium-transporting ATPase subunit alpha-4
XM_005203533.3	XP_005203590.1	sodium/potassium-transporting ATPase subunit alpha-4 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Atp1a4	NP_001257959.1
Homo sapiens (human)	ATP1A4	NP_653300.2
Pan troglodytes (chimpanzee)	ATP1A4	XP_001171135.1
	ATP1A4	XP_001117376.1
Canis lupus familiaris (dog)	ATP1A4	XP_545754.3
Mus musculus (house mouse)	Atp1a4	NP_038762.1
Bos taurus (cattle)	ATP1A4	NP_001137575.1

Gene Ontology
Bibliography

Related articles in PubMed

Testis-specific isoform of Na/K-ATPase (ATP1A4) regulates sperm function and fertility in dairy bulls through potential mechanisms involving reactive oxygen species, calcium and actin polymerization.
Rajamanickam GD, Kroetsch T, Kastelic JP, Thundathil JC
Andrology5(4)814-823(2017 Jul)

Content of testis-specific isoform of Na/K-ATPase (ATP1A4) is increased during bovine sperm capacitation through translation in mitochondrial ribosomes.
Rajamanickam GD, Kastelic JP, Thundathil JC
Cell and tissue research368(1)187-200(2017 04)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this is the first report demonstrating ATP1A4 synthesis during bovine sperm capacitation.
Title: Content of testis-specific isoform of Na/K-ATPase (ATP1A4) is increased during bovine sperm capacitation through translation in mitochondrial ribosomes.

The following ATP1A4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP1A4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa17174	NM_001144103.2 Latest version!	Bos taurus ATPase Na+/K+ transporting subunit alpha 4 (ATP1A4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa17174	XM_005203533.4 Latest version!	Bos taurus ATPase Na+/K+ transporting subunit alpha 4 (ATP1A4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa17174	XM_005203533.3	Bos taurus ATPase, Na+/K+ transporting, alpha 4 polypeptide (ATP1A4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP1A4 ( NM_001144103.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001137575.2 Bos taurus ATPase Na+/K+ transporting subunit alpha 4 (ATP1A4), mRNA.

Price & Availability↑

CloneID	OBa17174
Clone ID Related Accession (Same CDS sequence)	XM_005203533.4 , NM_001144103.2 , XM_005203533.3
Accession Version	NM_001144103.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	sodium/potassium-transporting ATPase subunit alpha-4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000003.1. On Feb 9, 2016 this sequence version replaced NM_001144103.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCTTG GAGGGAAAAA AGGGGCCGTG ACACCTCATG AGCGGAATCC AAACCCAGGA 
CCTACAAACA GTCCTAAGAT GAGTAAAAAA AAAATGAAGA GGAAAAAAAA GAAGAAAGAT 
ATAGAGGAGC TGAAGAAGGA AGTAGTCATG GATGATCACA AATTAACCTT GGATGAGCTG 
AGCGCCAAGT ACTCTGTGGA CCTGACCAGG GGCCATAGCC CTGAAAAGGC GCAGGAAATC 
CTGACTCGAG ATGGACCCAA TGCACTGACC CCTCCCTCTA CCACTCCAGA ATGGGTCAAA 
TTCTGCAAGC AGCTTTTCGG CGGCTTCTCC CTCCTGCTGT GGACTGGTGC CATTCTTTGC 
TTCGTGGCCT TCGGCATCCA GTTGTATTTC AATGAGGACA CCACCAAAGA CAACCTGTAC 
CTGGGCATTG TACTAACTGT CGTGGTCATC ATCACTGGCT GCTTCTCCTA TTATCAGGAA 
GCCAAGAGCT CCAAGATCAT GGAATCTTTT AAGAACATGG TACCTCAGCA AGCTCTGGTC 
ATTCGAGGTG GAGAGAAGTT TCAGATCCCT GTATACGAAG TGGTGGTAGG CGACCTGGTA 
GAAGTGAAGG GTGGGGATCG GATTCCCGCT GACATCCGGC TTATCTCTTC ACAAGGATGT 
AAGGTGGACA ACTCATCCTT GACAGGAGAG TCAGAGCCCC AGTCCCGCTC CACTGAGTTC 
ACCAATGAGA ACCCTCTGGA GACCCAAAAT ATCTGCTTCT TCTCCACCAA CTGTGTGGAA 
GGCTCAGCCC AAGGTATTGT GATTGCCACC GGAGACTCCA CCGTGATGGG CCGTATAGCC 
TCACTGACCT CCGGTCTGGC AGTGGGCAAG ACCCCCATTG CTATTGAGCT TGAACACTTC 
ATCCATCTGA TCACAGGGGT GGCCGTCTTC CTGGGTGTCA CTTTCTTTGG GCTCTCGCTT 
ATCTTGGGCT ACACCTGGCT GGAGGCTGTC ATTTTCCTTA TCGGCATCAT TGTGGCCAAC 
GTGCCTGAGG GACTGCTGGC CACCGTCACA GTATGCCTGA CCCTGACAGC CAAGCGCATG 
GCTCGGAAGA ACTGCCTGGT GAAGAACCTG GAGGCGGTGG AGACTCTGGG CTCCACCTCC 
ACCATCTGCT CTGACAAGAC GGGCACCCTC ACCCAGAACC GCATGACCGT CGCCCACCTG 
TGGTTCGACA AGACCATCTA CGAGGCGGAC ACCACTGAAG AACAGACTGG GAATCCATTT 
GCCAAGGGCT CTGACACCTG GTTTATCCTG GCCCGAATCG CTGGCCTCTG CAACCGGGCT 
GATTTTAAGG CGAATGAGGA GAGCCTTCCC ATAGCTAAGC GGGCAACAAC AGGAGATGCT 
TCGGAGTCAG CGCTCCTCAA GTTCATCGAG CAGTCCTACA GCTCTGTGAA CGAGATGAGA 
GAGAAAAGCC CCAAGGTGGC AGAGATTCCC TTTAATTCTA CCAACAAGTA CCAGGTGTCC 
ATCCACCTGA GGGAAGACAG CTCCCAGGCC CACGTCCTGA TGATGAAGGG GGCGCCCGAG 
AGGATCTTAG AATTTTGCTC TACTTACCTT CTGAAAGGGC AGGAGTACCC AATAGACGAT 
GAAATGAAGG ACGCCTTCCA AAACGCCTAC TTGGATCTGG GAGGTCTGGG GGAGCGTGTG 
CTAGGGTTCT GCTTCTTGAA TCTGCCTAAC ACTTACCCCA AGGGATTCAA GTTTAACACC 
GATGAAATCA ACTTTCCCAT AAATAACCTC TGTTTCGTGG GGCTCATCTC CATGATTGAC 
CCTCCCCGAG CTGCCGTGCC CGATGCTGTG GGCAAGTGCC GGAGTGCTGG GATTAAGGTG 
ATTATGGTAA CAGGGGATCA TCCCATCACA GCCAAGGCCA TTGCCAAGGG TGTGGGCATC 
ATCTCAGAGG GCTCTGAGAC AGCAGAGGAC ATTGCTGCCC GGCTCAAGAT CCCTGTCAGC 
AAGGTCAATC CTAGGGACGC CCAAGCCATT GTGGTGCATG GCTCAGAACT AAAGAATATG 
AACTCTGAGC AGCTTGATGA GATCCTCCAA AACCACACTG AGATCGTGTT CGCTCGGACC 
TCACCCCAGC AGAAGCTCAT CATTGTAGAG GGATGTCAGA GGCTGGGAGC CATTGTGGCA 
GTGACAGGGG ATGGGGTGAA CGACTCCCCG GCACTGAAGA AGGCGGACAT CGGCATCGCC 
ATGGGCATCG CCGGCTCCGA CGTGTCTAAG CAGGCCGCTG ACATGATCCT ACTGGATGAC 
AACTTTGCTT CCATTGTCAC GGGTGTGGAG GAGGGCCGCC TGATCTTTGA CAACCTGAAG 
AAATCCATCG CCTACACCCT GACCAGCAAC ATCCCCGAGA TCACCCCCTT CCTGATGTTC 
ATCATCCTCG GCATCCCCCT GCCTCTGGGC ACCATAACCA TCCTCTGCAT TGACCTGGGC 
ACTGACATGG TCCCTGCTAT CTCCCTGGCT TATGAGTCAG CTGAGAGTGA CATCATGAAG 
AGGGCTCCAC GGAACTCAAA GACTGATAAG CTAGTGAATC ACCGTCTCAT TGGCATGGCC 
TACGGACAGA TAGGGATGAT CCAGGCTCTG GCAGGATTCT TCACCTACTT TGTGATCTTG 
GCTGAGAATG GTTTTAAGCC TCCTGATCTG CTGGGCATCC GCGTCAACTG GGAAAATCGA 
TACATCAATG ACCTGGAGGA CAGCTATGGA CAGCAGTGGA CCTATGAGCA GCGGAAGGTG 
CTGGAGTTCA CGTGCCAAAC GGCCTTCTTC GTCAGCATCG TGATCGTGCA GTGGGCTGAC 
CTCATCATCT GTAAGACCCG TCGCAACTCA ATCTTCCAGC AGGGCATGAA AAACAAGATC 
CTCATATTTG GGATCCTGGA GGAGACAATC CTGGCCGCTT TTCTGTCCTA CACGCCAGGC 
ATGGATGTAG CTCTGCGAAT GTACCCACTC AAGATAACCT GGTGGTTCTG TGCCACCCCC 
TACAGCCTCC TCATCTTTGT GTATGATGAA ATAAGAAGAC TCATCATTCG TCGTTATCCC 
GGTGGCTGGG TGGAGAAGGA GACCTACTAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137575.2
CDS	1..3093
Translation	MGLGGKKGAVTPHERNPNPGPTNSPKMSKKKMKRKKKKKDIEELKKEVVMDDHKLTLDELSAKYSVDLTRGHSPEKAQEILTRDGPNALTPPSTTPEWVKFCKQLFGGFSLLLWTGAILCFVAFGIQLYFNEDTTKDNLYLGIVLTVVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRGGEKFQIPVYEVVVGDLVEVKGGDRIPADIRLISSQGCKVDNSSLTGESEPQSRSTEFTNENPLETQNICFFSTNCVEGSAQGIVIATGDSTVMGRIASLTSGLAVGKTPIAIELEHFIHLITGVAVFLGVTFFGLSLILGYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHLWFDKTIYEADTTEEQTGNPFAKGSDTWFILARIAGLCNRADFKANEESLPIAKRATTGDASESALLKFIEQSYSSVNEMREKSPKVAEIPFNSTNKYQVSIHLREDSSQAHVLMMKGAPERILEFCSTYLLKGQEYPIDDEMKDAFQNAYLDLGGLGERVLGFCFLNLPNTYPKGFKFNTDEINFPINNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGSETAEDIAARLKIPVSKVNPRDAQAIVVHGSELKNMNSEQLDEILQNHTEIVFARTSPQQKLIIVEGCQRLGAIVAVTGDGVNDSPALKKADIGIAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLMFIILGIPLPLGTITILCIDLGTDMVPAISLAYESAESDIMKRAPRNSKTDKLVNHRLIGMAYGQIGMIQALAGFFTYFVILAENGFKPPDLLGIRVNWENRYINDLEDSYGQQWTYEQRKVLEFTCQTAFFVSIVIVQWADLIICKTRRNSIFQQGMKNKILIFGILEETILAAFLSYTPGMDVALRMYPLKITWWFCATPYSLLIFVYDEIRRLIIRRYPGGWVEKETYY

Target ORF information:

RefSeq Version	NM_001144103.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ATPase Na+/K+ transporting subunit alpha 4 (ATP1A4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144103.2

ORF Insert Sequence:

ATGGGGCTTG GAGGGAAAAA AGGGGCCGTG ACACCTCATG AGCGGAATCC AAACCCAGGA 
CCTACAAACA GTCCTAAGAT GAGTAAAAAA AAAATGAAGA GGAAAAAAAA GAAGAAAGAT 
ATAGAGGAGC TGAAGAAGGA AGTAGTCATG GATGATCACA AATTAACCTT GGATGAGCTG 
AGCGCCAAGT ACTCTGTGGA CCTGACCAGG GGCCATAGCC CTGAAAAGGC GCAGGAAATC 
CTGACTCGAG ATGGACCCAA TGCACTGACC CCTCCCTCTA CCACTCCAGA ATGGGTCAAA 
TTCTGCAAGC AGCTTTTCGG CGGCTTCTCC CTCCTGCTGT GGACTGGTGC CATTCTTTGC 
TTCGTGGCCT TCGGCATCCA GTTGTATTTC AATGAGGACA CCACCAAAGA CAACCTGTAC 
CTGGGCATTG TACTAACTGT CGTGGTCATC ATCACTGGCT GCTTCTCCTA TTATCAGGAA 
GCCAAGAGCT CCAAGATCAT GGAATCTTTT AAGAACATGG TACCTCAGCA AGCTCTGGTC 
ATTCGAGGTG GAGAGAAGTT TCAGATCCCT GTATACGAAG TGGTGGTAGG CGACCTGGTA 
GAAGTGAAGG GTGGGGATCG GATTCCCGCT GACATCCGGC TTATCTCTTC ACAAGGATGT 
AAGGTGGACA ACTCATCCTT GACAGGAGAG TCAGAGCCCC AGTCCCGCTC CACTGAGTTC 
ACCAATGAGA ACCCTCTGGA GACCCAAAAT ATCTGCTTCT TCTCCACCAA CTGTGTGGAA 
GGCTCAGCCC AAGGTATTGT GATTGCCACC GGAGACTCCA CCGTGATGGG CCGTATAGCC 
TCACTGACCT CCGGTCTGGC AGTGGGCAAG ACCCCCATTG CTATTGAGCT TGAACACTTC 
ATCCATCTGA TCACAGGGGT GGCCGTCTTC CTGGGTGTCA CTTTCTTTGG GCTCTCGCTT 
ATCTTGGGCT ACACCTGGCT GGAGGCTGTC ATTTTCCTTA TCGGCATCAT TGTGGCCAAC 
GTGCCTGAGG GACTGCTGGC CACCGTCACA GTATGCCTGA CCCTGACAGC CAAGCGCATG 
GCTCGGAAGA ACTGCCTGGT GAAGAACCTG GAGGCGGTGG AGACTCTGGG CTCCACCTCC 
ACCATCTGCT CTGACAAGAC GGGCACCCTC ACCCAGAACC GCATGACCGT CGCCCACCTG 
TGGTTCGACA AGACCATCTA CGAGGCGGAC ACCACTGAAG AACAGACTGG GAATCCATTT 
GCCAAGGGCT CTGACACCTG GTTTATCCTG GCCCGAATCG CTGGCCTCTG CAACCGGGCT 
GATTTTAAGG CGAATGAGGA GAGCCTTCCC ATAGCTAAGC GGGCAACAAC AGGAGATGCT 
TCGGAGTCAG CGCTCCTCAA GTTCATCGAG CAGTCCTACA GCTCTGTGAA CGAGATGAGA 
GAGAAAAGCC CCAAGGTGGC AGAGATTCCC TTTAATTCTA CCAACAAGTA CCAGGTGTCC 
ATCCACCTGA GGGAAGACAG CTCCCAGGCC CACGTCCTGA TGATGAAGGG GGCGCCCGAG 
AGGATCTTAG AATTTTGCTC TACTTACCTT CTGAAAGGGC AGGAGTACCC AATAGACGAT 
GAAATGAAGG ACGCCTTCCA AAACGCCTAC TTGGATCTGG GAGGTCTGGG GGAGCGTGTG 
CTAGGGTTCT GCTTCTTGAA TCTGCCTAAC ACTTACCCCA AGGGATTCAA GTTTAACACC 
GATGAAATCA ACTTTCCCAT AAATAACCTC TGTTTCGTGG GGCTCATCTC CATGATTGAC 
CCTCCCCGAG CTGCCGTGCC CGATGCTGTG GGCAAGTGCC GGAGTGCTGG GATTAAGGTG 
ATTATGGTAA CAGGGGATCA TCCCATCACA GCCAAGGCCA TTGCCAAGGG TGTGGGCATC 
ATCTCAGAGG GCTCTGAGAC AGCAGAGGAC ATTGCTGCCC GGCTCAAGAT CCCTGTCAGC 
AAGGTCAATC CTAGGGACGC CCAAGCCATT GTGGTGCATG GCTCAGAACT AAAGAATATG 
AACTCTGAGC AGCTTGATGA GATCCTCCAA AACCACACTG AGATCGTGTT CGCTCGGACC 
TCACCCCAGC AGAAGCTCAT CATTGTAGAG GGATGTCAGA GGCTGGGAGC CATTGTGGCA 
GTGACAGGGG ATGGGGTGAA CGACTCCCCG GCACTGAAGA AGGCGGACAT CGGCATCGCC 
ATGGGCATCG CCGGCTCCGA CGTGTCTAAG CAGGCCGCTG ACATGATCCT ACTGGATGAC 
AACTTTGCTT CCATTGTCAC GGGTGTGGAG GAGGGCCGCC TGATCTTTGA CAACCTGAAG 
AAATCCATCG CCTACACCCT GACCAGCAAC ATCCCCGAGA TCACCCCCTT CCTGATGTTC 
ATCATCCTCG GCATCCCCCT GCCTCTGGGC ACCATAACCA TCCTCTGCAT TGACCTGGGC 
ACTGACATGG TCCCTGCTAT CTCCCTGGCT TATGAGTCAG CTGAGAGTGA CATCATGAAG 
AGGGCTCCAC GGAACTCAAA GACTGATAAG CTAGTGAATC ACCGTCTCAT TGGCATGGCC 
TACGGACAGA TAGGGATGAT CCAGGCTCTG GCAGGATTCT TCACCTACTT TGTGATCTTG 
GCTGAGAATG GTTTTAAGCC TCCTGATCTG CTGGGCATCC GCGTCAACTG GGAAAATCGA 
TACATCAATG ACCTGGAGGA CAGCTATGGA CAGCAGTGGA CCTATGAGCA GCGGAAGGTG 
CTGGAGTTCA CGTGCCAAAC GGCCTTCTTC GTCAGCATCG TGATCGTGCA GTGGGCTGAC 
CTCATCATCT GTAAGACCCG TCGCAACTCA ATCTTCCAGC AGGGCATGAA AAACAAGATC 
CTCATATTTG GGATCCTGGA GGAGACAATC CTGGCCGCTT TTCTGTCCTA CACGCCAGGC 
ATGGATGTAG CTCTGCGAAT GTACCCACTC AAGATAACCT GGTGGTTCTG TGCCACCCCC 
TACAGCCTCC TCATCTTTGT GTATGATGAA ATAAGAAGAC TCATCATTCG TCGTTATCCC 
GGTGGCTGGG TGGAGAAGGA GACCTACTAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP1A4 ( XM_005203533.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203590.1 Bos taurus ATPase Na+/K+ transporting subunit alpha 4 (ATP1A4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa17174
Clone ID Related Accession (Same CDS sequence)	XM_005203533.4 , NM_001144103.2 , XM_005203533.3
Accession Version	XM_005203533.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium/potassium-transporting ATPase subunit alpha-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203533.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTTG GAGGGAAAAA AGGGGCCGTG ACACCTCATG AGCGGAATCC AAACCCAGGA 
CCTACAAACA GTCCTAAGAT GAGTAAAAAA AAAATGAAGA GGAAAAAAAA GAAGAAAGAT 
ATAGAGGAGC TGAAGAAGGA AGTAGTCATG GATGATCACA AATTAACCTT GGATGAGCTG 
AGCGCCAAGT ACTCTGTGGA CCTGACCAGG GGCCATAGCC CTGAAAAGGC GCAGGAAATC 
CTGACTCGAG ATGGACCCAA TGCACTGACC CCTCCCTCTA CCACTCCAGA ATGGGTCAAA 
TTCTGCAAGC AGCTTTTCGG CGGCTTCTCC CTCCTGCTGT GGACTGGTGC CATTCTTTGC 
TTCGTGGCCT TCGGCATCCA GTTGTATTTC AATGAGGACA CCACCAAAGA CAACCTGTAC 
CTGGGCATTG TACTAACTGT CGTGGTCATC ATCACTGGCT GCTTCTCCTA TTATCAGGAA 
GCCAAGAGCT CCAAGATCAT GGAATCTTTT AAGAACATGG TACCTCAGCA AGCTCTGGTC 
ATTCGAGGTG GAGAGAAGTT TCAGATCCCT GTATACGAAG TGGTGGTAGG CGACCTGGTA 
GAAGTGAAGG GTGGGGATCG GATTCCCGCT GACATCCGGC TTATCTCTTC ACAAGGATGT 
AAGGTGGACA ACTCATCCTT GACAGGAGAG TCAGAGCCCC AGTCCCGCTC CACTGAGTTC 
ACCAATGAGA ACCCTCTGGA GACCCAAAAT ATCTGCTTCT TCTCCACCAA CTGTGTGGAA 
GGCTCAGCCC AAGGTATTGT GATTGCCACC GGAGACTCCA CCGTGATGGG CCGTATAGCC 
TCACTGACCT CCGGTCTGGC AGTGGGCAAG ACCCCCATTG CTATTGAGCT TGAACACTTC 
ATCCATCTGA TCACAGGGGT GGCCGTCTTC CTGGGTGTCA CTTTCTTTGG GCTCTCGCTT 
ATCTTGGGCT ACACCTGGCT GGAGGCTGTC ATTTTCCTTA TCGGCATCAT TGTGGCCAAC 
GTGCCTGAGG GACTGCTGGC CACCGTCACA GTATGCCTGA CCCTGACAGC CAAGCGCATG 
GCTCGGAAGA ACTGCCTGGT GAAGAACCTG GAGGCGGTGG AGACTCTGGG CTCCACCTCC 
ACCATCTGCT CTGACAAGAC GGGCACCCTC ACCCAGAACC GCATGACCGT CGCCCACCTG 
TGGTTCGACA AGACCATCTA CGAGGCGGAC ACCACTGAAG AACAGACTGG GAATCCATTT 
GCCAAGGGCT CTGACACCTG GTTTATCCTG GCCCGAATCG CTGGCCTCTG CAACCGGGCT 
GATTTTAAGG CGAATGAGGA GAGCCTTCCC ATAGCTAAGC GGGCAACAAC AGGAGATGCT 
TCGGAGTCAG CGCTCCTCAA GTTCATCGAG CAGTCCTACA GCTCTGTGAA CGAGATGAGA 
GAGAAAAGCC CCAAGGTGGC AGAGATTCCC TTTAATTCTA CCAACAAGTA CCAGGTGTCC 
ATCCACCTGA GGGAAGACAG CTCCCAGGCC CACGTCCTGA TGATGAAGGG GGCGCCCGAG 
AGGATCTTAG AATTTTGCTC TACTTACCTT CTGAAAGGGC AGGAGTACCC AATAGACGAT 
GAAATGAAGG ACGCCTTCCA AAACGCCTAC TTGGATCTGG GAGGTCTGGG GGAGCGTGTG 
CTAGGGTTCT GCTTCTTGAA TCTGCCTAAC ACTTACCCCA AGGGATTCAA GTTTAACACC 
GATGAAATCA ACTTTCCCAT AAATAACCTC TGTTTCGTGG GGCTCATCTC CATGATTGAC 
CCTCCCCGAG CTGCCGTGCC CGATGCTGTG GGCAAGTGCC GGAGTGCTGG GATTAAGGTG 
ATTATGGTAA CAGGGGATCA TCCCATCACA GCCAAGGCCA TTGCCAAGGG TGTGGGCATC 
ATCTCAGAGG GCTCTGAGAC AGCAGAGGAC ATTGCTGCCC GGCTCAAGAT CCCTGTCAGC 
AAGGTCAATC CTAGGGACGC CCAAGCCATT GTGGTGCATG GCTCAGAACT AAAGAATATG 
AACTCTGAGC AGCTTGATGA GATCCTCCAA AACCACACTG AGATCGTGTT CGCTCGGACC 
TCACCCCAGC AGAAGCTCAT CATTGTAGAG GGATGTCAGA GGCTGGGAGC CATTGTGGCA 
GTGACAGGGG ATGGGGTGAA CGACTCCCCG GCACTGAAGA AGGCGGACAT CGGCATCGCC 
ATGGGCATCG CCGGCTCCGA CGTGTCTAAG CAGGCCGCTG ACATGATCCT ACTGGATGAC 
AACTTTGCTT CCATTGTCAC GGGTGTGGAG GAGGGCCGCC TGATCTTTGA CAACCTGAAG 
AAATCCATCG CCTACACCCT GACCAGCAAC ATCCCCGAGA TCACCCCCTT CCTGATGTTC 
ATCATCCTCG GCATCCCCCT GCCTCTGGGC ACCATAACCA TCCTCTGCAT TGACCTGGGC 
ACTGACATGG TCCCTGCTAT CTCCCTGGCT TATGAGTCAG CTGAGAGTGA CATCATGAAG 
AGGGCTCCAC GGAACTCAAA GACTGATAAG CTAGTGAATC ACCGTCTCAT TGGCATGGCC 
TACGGACAGA TAGGGATGAT CCAGGCTCTG GCAGGATTCT TCACCTACTT TGTGATCTTG 
GCTGAGAATG GTTTTAAGCC TCCTGATCTG CTGGGCATCC GCGTCAACTG GGAAAATCGA 
TACATCAATG ACCTGGAGGA CAGCTATGGA CAGCAGTGGA CCTATGAGCA GCGGAAGGTG 
CTGGAGTTCA CGTGCCAAAC GGCCTTCTTC GTCAGCATCG TGATCGTGCA GTGGGCTGAC 
CTCATCATCT GTAAGACCCG TCGCAACTCA ATCTTCCAGC AGGGCATGAA AAACAAGATC 
CTCATATTTG GGATCCTGGA GGAGACAATC CTGGCCGCTT TTCTGTCCTA CACGCCAGGC 
ATGGATGTAG CTCTGCGAAT GTACCCACTC AAGATAACCT GGTGGTTCTG TGCCACCCCC 
TACAGCCTCC TCATCTTTGT GTATGATGAA ATAAGAAGAC TCATCATTCG TCGTTATCCC 
GGTGGCTGGG TGGAGAAGGA GACCTACTAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203590.1
CDS	145..3237
Translation	MGLGGKKGAVTPHERNPNPGPTNSPKMSKKKMKRKKKKKDIEELKKEVVMDDHKLTLDELSAKYSVDLTRGHSPEKAQEILTRDGPNALTPPSTTPEWVKFCKQLFGGFSLLLWTGAILCFVAFGIQLYFNEDTTKDNLYLGIVLTVVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRGGEKFQIPVYEVVVGDLVEVKGGDRIPADIRLISSQGCKVDNSSLTGESEPQSRSTEFTNENPLETQNICFFSTNCVEGSAQGIVIATGDSTVMGRIASLTSGLAVGKTPIAIELEHFIHLITGVAVFLGVTFFGLSLILGYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHLWFDKTIYEADTTEEQTGNPFAKGSDTWFILARIAGLCNRADFKANEESLPIAKRATTGDASESALLKFIEQSYSSVNEMREKSPKVAEIPFNSTNKYQVSIHLREDSSQAHVLMMKGAPERILEFCSTYLLKGQEYPIDDEMKDAFQNAYLDLGGLGERVLGFCFLNLPNTYPKGFKFNTDEINFPINNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGSETAEDIAARLKIPVSKVNPRDAQAIVVHGSELKNMNSEQLDEILQNHTEIVFARTSPQQKLIIVEGCQRLGAIVAVTGDGVNDSPALKKADIGIAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLMFIILGIPLPLGTITILCIDLGTDMVPAISLAYESAESDIMKRAPRNSKTDKLVNHRLIGMAYGQIGMIQALAGFFTYFVILAENGFKPPDLLGIRVNWENRYINDLEDSYGQQWTYEQRKVLEFTCQTAFFVSIVIVQWADLIICKTRRNSIFQQGMKNKILIFGILEETILAAFLSYTPGMDVALRMYPLKITWWFCATPYSLLIFVYDEIRRLIIRRYPGGWVEKETYY

Target ORF information:

RefSeq Version	XM_005203533.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ATPase Na+/K+ transporting subunit alpha 4 (ATP1A4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203533.4

ORF Insert Sequence:

ATGGGGCTTG GAGGGAAAAA AGGGGCCGTG ACACCTCATG AGCGGAATCC AAACCCAGGA 
CCTACAAACA GTCCTAAGAT GAGTAAAAAA AAAATGAAGA GGAAAAAAAA GAAGAAAGAT 
ATAGAGGAGC TGAAGAAGGA AGTAGTCATG GATGATCACA AATTAACCTT GGATGAGCTG 
AGCGCCAAGT ACTCTGTGGA CCTGACCAGG GGCCATAGCC CTGAAAAGGC GCAGGAAATC 
CTGACTCGAG ATGGACCCAA TGCACTGACC CCTCCCTCTA CCACTCCAGA ATGGGTCAAA 
TTCTGCAAGC AGCTTTTCGG CGGCTTCTCC CTCCTGCTGT GGACTGGTGC CATTCTTTGC 
TTCGTGGCCT TCGGCATCCA GTTGTATTTC AATGAGGACA CCACCAAAGA CAACCTGTAC 
CTGGGCATTG TACTAACTGT CGTGGTCATC ATCACTGGCT GCTTCTCCTA TTATCAGGAA 
GCCAAGAGCT CCAAGATCAT GGAATCTTTT AAGAACATGG TACCTCAGCA AGCTCTGGTC 
ATTCGAGGTG GAGAGAAGTT TCAGATCCCT GTATACGAAG TGGTGGTAGG CGACCTGGTA 
GAAGTGAAGG GTGGGGATCG GATTCCCGCT GACATCCGGC TTATCTCTTC ACAAGGATGT 
AAGGTGGACA ACTCATCCTT GACAGGAGAG TCAGAGCCCC AGTCCCGCTC CACTGAGTTC 
ACCAATGAGA ACCCTCTGGA GACCCAAAAT ATCTGCTTCT TCTCCACCAA CTGTGTGGAA 
GGCTCAGCCC AAGGTATTGT GATTGCCACC GGAGACTCCA CCGTGATGGG CCGTATAGCC 
TCACTGACCT CCGGTCTGGC AGTGGGCAAG ACCCCCATTG CTATTGAGCT TGAACACTTC 
ATCCATCTGA TCACAGGGGT GGCCGTCTTC CTGGGTGTCA CTTTCTTTGG GCTCTCGCTT 
ATCTTGGGCT ACACCTGGCT GGAGGCTGTC ATTTTCCTTA TCGGCATCAT TGTGGCCAAC 
GTGCCTGAGG GACTGCTGGC CACCGTCACA GTATGCCTGA CCCTGACAGC CAAGCGCATG 
GCTCGGAAGA ACTGCCTGGT GAAGAACCTG GAGGCGGTGG AGACTCTGGG CTCCACCTCC 
ACCATCTGCT CTGACAAGAC GGGCACCCTC ACCCAGAACC GCATGACCGT CGCCCACCTG 
TGGTTCGACA AGACCATCTA CGAGGCGGAC ACCACTGAAG AACAGACTGG GAATCCATTT 
GCCAAGGGCT CTGACACCTG GTTTATCCTG GCCCGAATCG CTGGCCTCTG CAACCGGGCT 
GATTTTAAGG CGAATGAGGA GAGCCTTCCC ATAGCTAAGC GGGCAACAAC AGGAGATGCT 
TCGGAGTCAG CGCTCCTCAA GTTCATCGAG CAGTCCTACA GCTCTGTGAA CGAGATGAGA 
GAGAAAAGCC CCAAGGTGGC AGAGATTCCC TTTAATTCTA CCAACAAGTA CCAGGTGTCC 
ATCCACCTGA GGGAAGACAG CTCCCAGGCC CACGTCCTGA TGATGAAGGG GGCGCCCGAG 
AGGATCTTAG AATTTTGCTC TACTTACCTT CTGAAAGGGC AGGAGTACCC AATAGACGAT 
GAAATGAAGG ACGCCTTCCA AAACGCCTAC TTGGATCTGG GAGGTCTGGG GGAGCGTGTG 
CTAGGGTTCT GCTTCTTGAA TCTGCCTAAC ACTTACCCCA AGGGATTCAA GTTTAACACC 
GATGAAATCA ACTTTCCCAT AAATAACCTC TGTTTCGTGG GGCTCATCTC CATGATTGAC 
CCTCCCCGAG CTGCCGTGCC CGATGCTGTG GGCAAGTGCC GGAGTGCTGG GATTAAGGTG 
ATTATGGTAA CAGGGGATCA TCCCATCACA GCCAAGGCCA TTGCCAAGGG TGTGGGCATC 
ATCTCAGAGG GCTCTGAGAC AGCAGAGGAC ATTGCTGCCC GGCTCAAGAT CCCTGTCAGC 
AAGGTCAATC CTAGGGACGC CCAAGCCATT GTGGTGCATG GCTCAGAACT AAAGAATATG 
AACTCTGAGC AGCTTGATGA GATCCTCCAA AACCACACTG AGATCGTGTT CGCTCGGACC 
TCACCCCAGC AGAAGCTCAT CATTGTAGAG GGATGTCAGA GGCTGGGAGC CATTGTGGCA 
GTGACAGGGG ATGGGGTGAA CGACTCCCCG GCACTGAAGA AGGCGGACAT CGGCATCGCC 
ATGGGCATCG CCGGCTCCGA CGTGTCTAAG CAGGCCGCTG ACATGATCCT ACTGGATGAC 
AACTTTGCTT CCATTGTCAC GGGTGTGGAG GAGGGCCGCC TGATCTTTGA CAACCTGAAG 
AAATCCATCG CCTACACCCT GACCAGCAAC ATCCCCGAGA TCACCCCCTT CCTGATGTTC 
ATCATCCTCG GCATCCCCCT GCCTCTGGGC ACCATAACCA TCCTCTGCAT TGACCTGGGC 
ACTGACATGG TCCCTGCTAT CTCCCTGGCT TATGAGTCAG CTGAGAGTGA CATCATGAAG 
AGGGCTCCAC GGAACTCAAA GACTGATAAG CTAGTGAATC ACCGTCTCAT TGGCATGGCC 
TACGGACAGA TAGGGATGAT CCAGGCTCTG GCAGGATTCT TCACCTACTT TGTGATCTTG 
GCTGAGAATG GTTTTAAGCC TCCTGATCTG CTGGGCATCC GCGTCAACTG GGAAAATCGA 
TACATCAATG ACCTGGAGGA CAGCTATGGA CAGCAGTGGA CCTATGAGCA GCGGAAGGTG 
CTGGAGTTCA CGTGCCAAAC GGCCTTCTTC GTCAGCATCG TGATCGTGCA GTGGGCTGAC 
CTCATCATCT GTAAGACCCG TCGCAACTCA ATCTTCCAGC AGGGCATGAA AAACAAGATC 
CTCATATTTG GGATCCTGGA GGAGACAATC CTGGCCGCTT TTCTGTCCTA CACGCCAGGC 
ATGGATGTAG CTCTGCGAAT GTACCCACTC AAGATAACCT GGTGGTTCTG TGCCACCCCC 
TACAGCCTCC TCATCTTTGT GTATGATGAA ATAAGAAGAC TCATCATTCG TCGTTATCCC 
GGTGGCTGGG TGGAGAAGGA GACCTACTAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP1A4 ( XM_005203533.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203590.1 Bos taurus ATPase, Na+/K+ transporting, alpha 4 polypeptide (ATP1A4), transcript variant X1, mRNA.

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CloneID	OBa17174
Clone ID Related Accession (Same CDS sequence)	XM_005203533.4 , NM_001144103.2 , XM_005203533.3
Accession Version	XM_005203533.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sodium/potassium-transporting ATPase subunit alpha-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103856.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203533.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTTG GAGGGAAAAA AGGGGCCGTG ACACCTCATG AGCGGAATCC AAACCCAGGA 
CCTACAAACA GTCCTAAGAT GAGTAAAAAA AAAATGAAGA GGAAAAAAAA GAAGAAAGAT 
ATAGAGGAGC TGAAGAAGGA AGTAGTCATG GATGATCACA AATTAACCTT GGATGAGCTG 
AGCGCCAAGT ACTCTGTGGA CCTGACCAGG GGCCATAGCC CTGAAAAGGC GCAGGAAATC 
CTGACTCGAG ATGGACCCAA TGCACTGACC CCTCCCTCTA CCACTCCAGA ATGGGTCAAA 
TTCTGCAAGC AGCTTTTCGG CGGCTTCTCC CTCCTGCTGT GGACTGGTGC CATTCTTTGC 
TTCGTGGCCT TCGGCATCCA GTTGTATTTC AATGAGGACA CCACCAAAGA CAACCTGTAC 
CTGGGCATTG TACTAACTGT CGTGGTCATC ATCACTGGCT GCTTCTCCTA TTATCAGGAA 
GCCAAGAGCT CCAAGATCAT GGAATCTTTT AAGAACATGG TACCTCAGCA AGCTCTGGTC 
ATTCGAGGTG GAGAGAAGTT TCAGATCCCT GTATACGAAG TGGTGGTAGG CGACCTGGTA 
GAAGTGAAGG GTGGGGATCG GATTCCCGCT GACATCCGGC TTATCTCTTC ACAAGGATGT 
AAGGTGGACA ACTCATCCTT GACAGGAGAG TCAGAGCCCC AGTCCCGCTC CACTGAGTTC 
ACCAATGAGA ACCCTCTGGA GACCCAAAAT ATCTGCTTCT TCTCCACCAA CTGTGTGGAA 
GGCTCAGCCC AAGGTATTGT GATTGCCACC GGAGACTCCA CCGTGATGGG CCGTATAGCC 
TCACTGACCT CCGGTCTGGC AGTGGGCAAG ACCCCCATTG CTATTGAGCT TGAACACTTC 
ATCCATCTGA TCACAGGGGT GGCCGTCTTC CTGGGTGTCA CTTTCTTTGG GCTCTCGCTT 
ATCTTGGGCT ACACCTGGCT GGAGGCTGTC ATTTTCCTTA TCGGCATCAT TGTGGCCAAC 
GTGCCTGAGG GACTGCTGGC CACCGTCACA GTATGCCTGA CCCTGACAGC CAAGCGCATG 
GCTCGGAAGA ACTGCCTGGT GAAGAACCTG GAGGCGGTGG AGACTCTGGG CTCCACCTCC 
ACCATCTGCT CTGACAAGAC GGGCACCCTC ACCCAGAACC GCATGACCGT CGCCCACCTG 
TGGTTCGACA AGACCATCTA CGAGGCGGAC ACCACTGAAG AACAGACTGG GAATCCATTT 
GCCAAGGGCT CTGACACCTG GTTTATCCTG GCCCGAATCG CTGGCCTCTG CAACCGGGCT 
GATTTTAAGG CGAATGAGGA GAGCCTTCCC ATAGCTAAGC GGGCAACAAC AGGAGATGCT 
TCGGAGTCAG CGCTCCTCAA GTTCATCGAG CAGTCCTACA GCTCTGTGAA CGAGATGAGA 
GAGAAAAGCC CCAAGGTGGC AGAGATTCCC TTTAATTCTA CCAACAAGTA CCAGGTGTCC 
ATCCACCTGA GGGAAGACAG CTCCCAGGCC CACGTCCTGA TGATGAAGGG GGCGCCCGAG 
AGGATCTTAG AATTTTGCTC TACTTACCTT CTGAAAGGGC AGGAGTACCC AATAGACGAT 
GAAATGAAGG ACGCCTTCCA AAACGCCTAC TTGGATCTGG GAGGTCTGGG GGAGCGTGTG 
CTAGGGTTCT GCTTCTTGAA TCTGCCTAAC ACTTACCCCA AGGGATTCAA GTTTAACACC 
GATGAAATCA ACTTTCCCAT AAATAACCTC TGTTTCGTGG GGCTCATCTC CATGATTGAC 
CCTCCCCGAG CTGCCGTGCC CGATGCTGTG GGCAAGTGCC GGAGTGCTGG GATTAAGGTG 
ATTATGGTAA CAGGGGATCA TCCCATCACA GCCAAGGCCA TTGCCAAGGG TGTGGGCATC 
ATCTCAGAGG GCTCTGAGAC AGCAGAGGAC ATTGCTGCCC GGCTCAAGAT CCCTGTCAGC 
AAGGTCAATC CTAGGGACGC CCAAGCCATT GTGGTGCATG GCTCAGAACT AAAGAATATG 
AACTCTGAGC AGCTTGATGA GATCCTCCAA AACCACACTG AGATCGTGTT CGCTCGGACC 
TCACCCCAGC AGAAGCTCAT CATTGTAGAG GGATGTCAGA GGCTGGGAGC CATTGTGGCA 
GTGACAGGGG ATGGGGTGAA CGACTCCCCG GCACTGAAGA AGGCGGACAT CGGCATCGCC 
ATGGGCATCG CCGGCTCCGA CGTGTCTAAG CAGGCCGCTG ACATGATCCT ACTGGATGAC 
AACTTTGCTT CCATTGTCAC GGGTGTGGAG GAGGGCCGCC TGATCTTTGA CAACCTGAAG 
AAATCCATCG CCTACACCCT GACCAGCAAC ATCCCCGAGA TCACCCCCTT CCTGATGTTC 
ATCATCCTCG GCATCCCCCT GCCTCTGGGC ACCATAACCA TCCTCTGCAT TGACCTGGGC 
ACTGACATGG TCCCTGCTAT CTCCCTGGCT TATGAGTCAG CTGAGAGTGA CATCATGAAG 
AGGGCTCCAC GGAACTCAAA GACTGATAAG CTAGTGAATC ACCGTCTCAT TGGCATGGCC 
TACGGACAGA TAGGGATGAT CCAGGCTCTG GCAGGATTCT TCACCTACTT TGTGATCTTG 
GCTGAGAATG GTTTTAAGCC TCCTGATCTG CTGGGCATCC GCGTCAACTG GGAAAATCGA 
TACATCAATG ACCTGGAGGA CAGCTATGGA CAGCAGTGGA CCTATGAGCA GCGGAAGGTG 
CTGGAGTTCA CGTGCCAAAC GGCCTTCTTC GTCAGCATCG TGATCGTGCA GTGGGCTGAC 
CTCATCATCT GTAAGACCCG TCGCAACTCA ATCTTCCAGC AGGGCATGAA AAACAAGATC 
CTCATATTTG GGATCCTGGA GGAGACAATC CTGGCCGCTT TTCTGTCCTA CACGCCAGGC 
ATGGATGTAG CTCTGCGAAT GTACCCACTC AAGATAACCT GGTGGTTCTG TGCCACCCCC 
TACAGCCTCC TCATCTTTGT GTATGATGAA ATAAGAAGAC TCATCATTCG TCGTTATCCC 
GGTGGCTGGG TGGAGAAGGA GACCTACTAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203590.1
CDS	108..3200
Translation	MGLGGKKGAVTPHERNPNPGPTNSPKMSKKKMKRKKKKKDIEELKKEVVMDDHKLTLDELSAKYSVDLTRGHSPEKAQEILTRDGPNALTPPSTTPEWVKFCKQLFGGFSLLLWTGAILCFVAFGIQLYFNEDTTKDNLYLGIVLTVVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRGGEKFQIPVYEVVVGDLVEVKGGDRIPADIRLISSQGCKVDNSSLTGESEPQSRSTEFTNENPLETQNICFFSTNCVEGSAQGIVIATGDSTVMGRIASLTSGLAVGKTPIAIELEHFIHLITGVAVFLGVTFFGLSLILGYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHLWFDKTIYEADTTEEQTGNPFAKGSDTWFILARIAGLCNRADFKANEESLPIAKRATTGDASESALLKFIEQSYSSVNEMREKSPKVAEIPFNSTNKYQVSIHLREDSSQAHVLMMKGAPERILEFCSTYLLKGQEYPIDDEMKDAFQNAYLDLGGLGERVLGFCFLNLPNTYPKGFKFNTDEINFPINNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGSETAEDIAARLKIPVSKVNPRDAQAIVVHGSELKNMNSEQLDEILQNHTEIVFARTSPQQKLIIVEGCQRLGAIVAVTGDGVNDSPALKKADIGIAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLMFIILGIPLPLGTITILCIDLGTDMVPAISLAYESAESDIMKRAPRNSKTDKLVNHRLIGMAYGQIGMIQALAGFFTYFVILAENGFKPPDLLGIRVNWENRYINDLEDSYGQQWTYEQRKVLEFTCQTAFFVSIVIVQWADLIICKTRRNSIFQQGMKNKILIFGILEETILAAFLSYTPGMDVALRMYPLKITWWFCATPYSLLIFVYDEIRRLIIRRYPGGWVEKETYY

Target ORF information:

RefSeq Version	XM_005203533.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ATPase, Na+/K+ transporting, alpha 4 polypeptide (ATP1A4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203533.3

ORF Insert Sequence:

ATGGGGCTTG GAGGGAAAAA AGGGGCCGTG ACACCTCATG AGCGGAATCC AAACCCAGGA 
CCTACAAACA GTCCTAAGAT GAGTAAAAAA AAAATGAAGA GGAAAAAAAA GAAGAAAGAT 
ATAGAGGAGC TGAAGAAGGA AGTAGTCATG GATGATCACA AATTAACCTT GGATGAGCTG 
AGCGCCAAGT ACTCTGTGGA CCTGACCAGG GGCCATAGCC CTGAAAAGGC GCAGGAAATC 
CTGACTCGAG ATGGACCCAA TGCACTGACC CCTCCCTCTA CCACTCCAGA ATGGGTCAAA 
TTCTGCAAGC AGCTTTTCGG CGGCTTCTCC CTCCTGCTGT GGACTGGTGC CATTCTTTGC 
TTCGTGGCCT TCGGCATCCA GTTGTATTTC AATGAGGACA CCACCAAAGA CAACCTGTAC 
CTGGGCATTG TACTAACTGT CGTGGTCATC ATCACTGGCT GCTTCTCCTA TTATCAGGAA 
GCCAAGAGCT CCAAGATCAT GGAATCTTTT AAGAACATGG TACCTCAGCA AGCTCTGGTC 
ATTCGAGGTG GAGAGAAGTT TCAGATCCCT GTATACGAAG TGGTGGTAGG CGACCTGGTA 
GAAGTGAAGG GTGGGGATCG GATTCCCGCT GACATCCGGC TTATCTCTTC ACAAGGATGT 
AAGGTGGACA ACTCATCCTT GACAGGAGAG TCAGAGCCCC AGTCCCGCTC CACTGAGTTC 
ACCAATGAGA ACCCTCTGGA GACCCAAAAT ATCTGCTTCT TCTCCACCAA CTGTGTGGAA 
GGCTCAGCCC AAGGTATTGT GATTGCCACC GGAGACTCCA CCGTGATGGG CCGTATAGCC 
TCACTGACCT CCGGTCTGGC AGTGGGCAAG ACCCCCATTG CTATTGAGCT TGAACACTTC 
ATCCATCTGA TCACAGGGGT GGCCGTCTTC CTGGGTGTCA CTTTCTTTGG GCTCTCGCTT 
ATCTTGGGCT ACACCTGGCT GGAGGCTGTC ATTTTCCTTA TCGGCATCAT TGTGGCCAAC 
GTGCCTGAGG GACTGCTGGC CACCGTCACA GTATGCCTGA CCCTGACAGC CAAGCGCATG 
GCTCGGAAGA ACTGCCTGGT GAAGAACCTG GAGGCGGTGG AGACTCTGGG CTCCACCTCC 
ACCATCTGCT CTGACAAGAC GGGCACCCTC ACCCAGAACC GCATGACCGT CGCCCACCTG 
TGGTTCGACA AGACCATCTA CGAGGCGGAC ACCACTGAAG AACAGACTGG GAATCCATTT 
GCCAAGGGCT CTGACACCTG GTTTATCCTG GCCCGAATCG CTGGCCTCTG CAACCGGGCT 
GATTTTAAGG CGAATGAGGA GAGCCTTCCC ATAGCTAAGC GGGCAACAAC AGGAGATGCT 
TCGGAGTCAG CGCTCCTCAA GTTCATCGAG CAGTCCTACA GCTCTGTGAA CGAGATGAGA 
GAGAAAAGCC CCAAGGTGGC AGAGATTCCC TTTAATTCTA CCAACAAGTA CCAGGTGTCC 
ATCCACCTGA GGGAAGACAG CTCCCAGGCC CACGTCCTGA TGATGAAGGG GGCGCCCGAG 
AGGATCTTAG AATTTTGCTC TACTTACCTT CTGAAAGGGC AGGAGTACCC AATAGACGAT 
GAAATGAAGG ACGCCTTCCA AAACGCCTAC TTGGATCTGG GAGGTCTGGG GGAGCGTGTG 
CTAGGGTTCT GCTTCTTGAA TCTGCCTAAC ACTTACCCCA AGGGATTCAA GTTTAACACC 
GATGAAATCA ACTTTCCCAT AAATAACCTC TGTTTCGTGG GGCTCATCTC CATGATTGAC 
CCTCCCCGAG CTGCCGTGCC CGATGCTGTG GGCAAGTGCC GGAGTGCTGG GATTAAGGTG 
ATTATGGTAA CAGGGGATCA TCCCATCACA GCCAAGGCCA TTGCCAAGGG TGTGGGCATC 
ATCTCAGAGG GCTCTGAGAC AGCAGAGGAC ATTGCTGCCC GGCTCAAGAT CCCTGTCAGC 
AAGGTCAATC CTAGGGACGC CCAAGCCATT GTGGTGCATG GCTCAGAACT AAAGAATATG 
AACTCTGAGC AGCTTGATGA GATCCTCCAA AACCACACTG AGATCGTGTT CGCTCGGACC 
TCACCCCAGC AGAAGCTCAT CATTGTAGAG GGATGTCAGA GGCTGGGAGC CATTGTGGCA 
GTGACAGGGG ATGGGGTGAA CGACTCCCCG GCACTGAAGA AGGCGGACAT CGGCATCGCC 
ATGGGCATCG CCGGCTCCGA CGTGTCTAAG CAGGCCGCTG ACATGATCCT ACTGGATGAC 
AACTTTGCTT CCATTGTCAC GGGTGTGGAG GAGGGCCGCC TGATCTTTGA CAACCTGAAG 
AAATCCATCG CCTACACCCT GACCAGCAAC ATCCCCGAGA TCACCCCCTT CCTGATGTTC 
ATCATCCTCG GCATCCCCCT GCCTCTGGGC ACCATAACCA TCCTCTGCAT TGACCTGGGC 
ACTGACATGG TCCCTGCTAT CTCCCTGGCT TATGAGTCAG CTGAGAGTGA CATCATGAAG 
AGGGCTCCAC GGAACTCAAA GACTGATAAG CTAGTGAATC ACCGTCTCAT TGGCATGGCC 
TACGGACAGA TAGGGATGAT CCAGGCTCTG GCAGGATTCT TCACCTACTT TGTGATCTTG 
GCTGAGAATG GTTTTAAGCC TCCTGATCTG CTGGGCATCC GCGTCAACTG GGAAAATCGA 
TACATCAATG ACCTGGAGGA CAGCTATGGA CAGCAGTGGA CCTATGAGCA GCGGAAGGTG 
CTGGAGTTCA CGTGCCAAAC GGCCTTCTTC GTCAGCATCG TGATCGTGCA GTGGGCTGAC 
CTCATCATCT GTAAGACCCG TCGCAACTCA ATCTTCCAGC AGGGCATGAA AAACAAGATC 
CTCATATTTG GGATCCTGGA GGAGACAATC CTGGCCGCTT TTCTGTCCTA CACGCCAGGC 
ATGGATGTAG CTCTGCGAAT GTACCCACTC AAGATAACCT GGTGGTTCTG TGCCACCCCC 
TACAGCCTCC TCATCTTTGT GTATGATGAA ATAAGAAGAC TCATCATTCG TCGTTATCCC 
GGTGGCTGGG TGGAGAAGGA GACCTACTAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Testis-specific isoform of Na/K-ATPase (ATP1A4) regulates sperm function and fertility in dairy bulls through potential mechanisms involving reactive oxygen species, calcium and actin polymerization.
Andrology5(4)814-823(2017 Jul)
Rajamanickam GD,Kroetsch T,Kastelic JP,Thundathil JC

Content of testis-specific isoform of Na/K-ATPase (ATP1A4) is increased during bovine sperm capacitation through translation in mitochondrial ribosomes.
Cell and tissue research368(1)187-200(2017 04)
Rajamanickam GD,Kastelic JP,Thundathil JC

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ATP1A4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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