DGKH cDNA ORF clone, Bos taurus(cattle)

The following DGKH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa221234 XM_025000025.1
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa92097 XM_015465680.1
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa92097 XM_015473791.1
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa221230 XM_025000021.1
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221231 XM_025000022.1
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221232 XM_025000023.1
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221233 XM_025000024.1
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa07196 XM_015465679.1
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa07196 XM_002691824.4
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa07196 XM_015473789.2
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa07196 XM_617702.7
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa07196 XM_015465676.1
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa07196 XM_010810743.2
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa07197 XM_005213691.3
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa07197 XM_015465681.1
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa53690 XM_015465678.1
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa53690 XM_010810744.2
Latest version!
Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa07196 XM_015473789.1 Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa221234
    Clone ID Related Accession (Same CDS sequence) XM_025000025.1
    Accession Version XM_025000025.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2985bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGCAATTGG AATCTCCACA TGAAGAGCGA GTATCCAATG CCTTACATTC CGTGGAAGTG
    GAGCTACAGA AGTTAACAGA GATTCCATGG CTTTATTATA TCTTACACCC GAATGAGGAT
    GAGGAGCCCC CCATGGATTG CACCAAGAGA AATAGCAGAA GCACGGTATT TCGTATTGTG
    CCCAAATTCA AAAAGGAGAA GGTTCAGAAG CAGAAGACAA GTTCACAGCC TGGACCTGGG
    GATACCGAAA GTGGGTCATG TGAAGCGAAT TCTCCAGGGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855793.1
    CDS326..3310
    Translation

    Target ORF information:

    RefSeq Version XM_025000025.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000025.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGCAATTGG AATCTCCACA TGAAGAGCGA GTATCCAATG CCTTACATTC CGTGGAAGTG
    GAGCTACAGA AGTTAACAGA GATTCCATGG CTTTATTATA TCTTACACCC GAATGAGGAT
    GAGGAGCCCC CCATGGATTG CACCAAGAGA AATAGCAGAA GCACGGTATT TCGTATTGTG
    CCCAAATTCA AAAAGGAGAA GGTTCAGAAG CAGAAGACAA GTTCACAGCC TGGACCTGGG
    GATACCGAAA GTGGGTCATG TGAAGCGAAT TCTCCAGGGA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92097
    Clone ID Related Accession (Same CDS sequence) XM_015465680.1 , XM_015473791.1
    Accession Version XM_015465680.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2715bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645949.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGGTGAGGA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321166.1
    CDS100..2814
    Translation

    Target ORF information:

    RefSeq Version XM_015465680.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465680.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGGTGAGGA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92097
    Clone ID Related Accession (Same CDS sequence) XM_015465680.1 , XM_015473791.1
    Accession Version XM_015473791.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2715bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGGTGAGGA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329277.1
    CDS100..2814
    Translation

    Target ORF information:

    RefSeq Version XM_015473791.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473791.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGGTGAGGA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221230
    Clone ID Related Accession (Same CDS sequence) XM_025000021.1
    Accession Version XM_025000021.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCCGC GGCACGTGCG CCCCTCGCCG GGCGCCCGCA GCCCGACCTG GGAGGAACCG 
    GAGTCGGACA GGATGGCCGG GGCCGGCAGC CAACACCACC ACCCGGGCGC GGCGGGAGGA
    GCGGCAGGCG CTGCGGTCAC CCCCGCCACT GTCCCGGCGG GGCCCGGAGA CGACTCGTCA
    GACAGCGAAG CGGAGCAGGA GGGACCCCAG AAGCTGATCC GCAAAGTGTC CACCTCGGGG
    CAGATCCGCA CCAAGACCAG TATTAAAGAG GGACAGCTGC TGAAGCAAAC CAGTTCCTTC
    CAAAGGTGGA AAAAGCGATA CTTCAAACTT CGAGGTCGTA CACTTTATTA TGCAAAGGAC
    TCAAAGTCTC TGATATTTGA TGAAGTTGAC CTCTCAGATG CCAGTGTAGC TGAAGCAAGC
    ACGAAAAACG CTAACAACAG CTTCACGATA ATCACTCCAT TTAGAAGACT AATGCTGTGC
    GCTGAGAACA GGAAAGAGAT GGAGGATTGG ATCAGCTCGC TGAAGTCTGT ACAGACCAGA
    GAACCTTACG AGGTGGCCCA GTTTAACGTG GAACATTTCT CAGGGATGCA TAACTGGTAC
    GCGTGCTCCC ACGCCCGCCC CACTTTCTGT AACGTGTGCC GAGAGAGTCT TTCTGGAGTC
    ACCTCCCACG GCCTGTCCTG CGAAGTGTGT AAATTCAAGG CTCACAAACG CTGTGCAGTG
    AGGGCAATGA ACAACTGCAA GTGGACGACC CTGGCCTCCA TCGGGAAGGA CATCATCGAG
    AACGAGGACG GGGTGGCTAT GCCTCACCAG TGGCTCGAGG GTAACTTGCC CGTGAGCGCC
    AAGTGTGCCG TCTGCGACAA AACGTGCGGC AGTGTTCTCC GTCTTCAAGA TTGGAGATGC
    CTTTGGTGTA GAACGATGGT ACACACTGCC TGTAAAGATT TATATCATCC TGTATGTCCA
    CTTGGCCAAT GTAAAGTATC TGTCATACCT CCAATTGCAC TCAACAGCAC TGATTCTGAT
    GGTTTCTGTA GAGCAACATT TTCATTCTGT GTTAGTCCTT TATTGGTTTT TGTCAATTCT
    AAGAGTGGAG ATAATCAGGG AGTAAAGTTT CTTCGTCGAT TTAAACAGTT ACTAAATCCA
    GCTCAGGTGT TTGATTTGAT GAATGGCGGT CCTCATTTGG GTTTAAGATT ATTCCAGAAG
    TTTGACAATT TCCGGATTCT TGTTTGTGGA GGAGATGGAA GTGTAGGTTG GGTTTTGTCA
    GAAATTGATA AGCTCAACTT GAACAAACAG TGTCAGCTGG GAGTACTGCC TCTGGGGACA
    GGAAACGACC TTGCCCGGGT TCTCGGCTGG GGAGGCTCGT ACGATGACGA CACCCAACTT
    CCTCAGATAC TTGAGAAGCT GGAACGAGCC AGTACCAAAA TGTTGGACAG GTGGAGTATT
    ATGACCTATG AACTCAAATT GCCGCCAAAA GCTTCTCTAC TTCCCGAACC TCCAGAGGCG
    TCTGAAGAAT TTTATATGAC AATTTATGAA GACTCAGTTG CAGCTCATCT TACAAAAATC
    CTCAATTCTG ATGAACATGC AGTGGTCATA TCTTCTACCA AGATACTCTG TGAGACTGTA
    AAGGACTTCG TGGCCAAAGT AGAAAAAGCA CACGAGAAAA CGCTGGAAAA AGCTGTTGCA
    GCTGATGCTG TGGCCAATAA ATGTTCCATC CTAAATGAGA AGCTTGAACA ACTGCTCCAG
    GCTTTGCACA TAGAGTCCCA GGCAGGTCCA GTTCTCCCAG GCGTCAGCCC TCTCAGGGTA
    GAAGATGCTG TGGAATCTTC CAGCGAAGAA TCCTTGTGTG AGAGCAAGGA ACATCTCTTG
    GATGACACTA CGAAACCCTC TTCCCAGAAA GCTGTCAAAC CAAGGGAAAT AATGCTGCGG
    GCAAACAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AGGCCAGAAA AGTGATGGAT
    GACCAGCCAG TTCACTCCTC TGAACTAGTT AATCAGTCAT TCGATTATGA TAGCACAGAA
    ACAGATGATT CCAAAGATGA GTTAAAAGAA GATGATGCGA AAGAGTCATT AACTGTTAAA
    GGTGCACCTC GGTCTCCGGA TGGCCGGGCT GGTCGTTCCC AAACTGACTC TGGCCCTGGT
    GCCCCTGTGG CAGCCAGCAA GGAAAACCTC CCTGTGCTCA ATACCAGAAT AATTTGCCCA
    GGTTTAAGAG CAGGACTAGC TGCCTCAATT GCTGGAAGTT CTATTATCAA CAAAATGTTG
    CTAGCAAATA TTGACCCTTT TGGTGCCACG CCATTTATTG ACCCAGATCC AGATTCAGTA
    GATGGCTATT CAGAAAAATG TGTCATGAAC AATTACTTTG GGATTGGATT AGATGCAAAA
    ATTTCATTAG AATTTAATAA TAAGAGAGAG GAGCACCCTG AAAAATGCAG GAGCCGAACT
    AAAAACTTGA TGTGGTATGG AGTCCTTGGA ACTCGGGAAT TATTACAAAG ATCATACAAG
    AATTTGGAAC AGAGGGTTCA ACTCGAATGT GACGGGCAGT ATATTCCTCT CCCCAGTCTG
    CAAGGAATAG CAGTGCTGAA CATCCCCAGC TATGCCGGAG GCACTAACTT TTGGGGTGGA
    ACTAAAGAAG ATGATATATT TGCTGCACCA TCATTTGATG ACAAGATCCT AGAAGTCGTT
    GCAATATTTG ACAGCGTGCA AATGGCCGTT TCAAGGGTCA TTAAACTGCA GCACCATCGA
    ATCGCCCAGT GCCGTACAGT AAAGATCACT ATCTTTGGTG ACGAGGGAGT CCCAGTGCAG
    GTGGACGGAG AAGCATGGGT TCAGCCTCCA GGGATTATCA AGATCGTGCA CAAGAACAGA
    GCTCAGATGC TCACCAGGGA CAGGGCCTTT GAAAGCACCC TGAAGTCTTG GGAAGATAAG
    CAGAAATGTG ATTCGGGTAA GCCAGTTCTC CGAACGCACT TGTACATCCA GCACGCGGCG
    GACCTGGCCA CGGAGGAAGT GTCTCAGATG CAGCTGTGCT CGCAGGCGGC CGAGGAGCTC
    ATCACCAGGA TCTGCGATGC GGCCACCATC CACTGTCTAT TGGAGCAGGA GCTGGCCCAC
    GCGGTGAACG CCTGCTCCCA CGCCCTGAAC AAAGCCAACC CACGCTTCCC AGAGAGTCTT
    ACAAGAGACA CTGCCACTGA AATAGCCATC AATGTGAAGG CCCTATATAA CGAAACAGAA
    TCTTTACTAG TGGGCAGGGT TCCTTTGCAA TTGGAATCTC CACATGAAGA GCGAGTATCC
    AATGCCTTAC ATTCCGTGGA AGTGGAGCTA CAGAAGTTAA CAGAGATTCC ATGGCTTTAT
    TATATCTTAC ACCCGAATGA GGATGAGGAG CCCCCCATGG ATTGCACCAA GAGAAATAGC
    AGAAGCACGG TATTTCGTAT TGTGCCCAAA TTCAAAAAGG AGAAGGTTCA GAAGCAGAAG
    ACAAGTTCAC AGCCTGTTCA GAAATGGGGC ACAGAGGAAG TTGCTGCTTG GCTGGATCTG
    CTCAATTTGG GAGAGTACAA AGAAATCTTC ATCCGCCATG ACATCAGAGG GGCTGAACTT
    TTGCATCTGG AAAGGCGAGA TCTTAAGGAC CTGGGGATAC CGAAAGTGGG TCATGTGAAG
    CGAATTCTCC AGGGAATTAA AGAGCTTGAA AGGAGCACCC TCCAGCCTGA GGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855789.1
    CDS36..3752
    Translation

    Target ORF information:

    RefSeq Version XM_025000021.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000021.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCCGC GGCACGTGCG CCCCTCGCCG GGCGCCCGCA GCCCGACCTG GGAGGAACCG 
    GAGTCGGACA GGATGGCCGG GGCCGGCAGC CAACACCACC ACCCGGGCGC GGCGGGAGGA
    GCGGCAGGCG CTGCGGTCAC CCCCGCCACT GTCCCGGCGG GGCCCGGAGA CGACTCGTCA
    GACAGCGAAG CGGAGCAGGA GGGACCCCAG AAGCTGATCC GCAAAGTGTC CACCTCGGGG
    CAGATCCGCA CCAAGACCAG TATTAAAGAG GGACAGCTGC TGAAGCAAAC CAGTTCCTTC
    CAAAGGTGGA AAAAGCGATA CTTCAAACTT CGAGGTCGTA CACTTTATTA TGCAAAGGAC
    TCAAAGTCTC TGATATTTGA TGAAGTTGAC CTCTCAGATG CCAGTGTAGC TGAAGCAAGC
    ACGAAAAACG CTAACAACAG CTTCACGATA ATCACTCCAT TTAGAAGACT AATGCTGTGC
    GCTGAGAACA GGAAAGAGAT GGAGGATTGG ATCAGCTCGC TGAAGTCTGT ACAGACCAGA
    GAACCTTACG AGGTGGCCCA GTTTAACGTG GAACATTTCT CAGGGATGCA TAACTGGTAC
    GCGTGCTCCC ACGCCCGCCC CACTTTCTGT AACGTGTGCC GAGAGAGTCT TTCTGGAGTC
    ACCTCCCACG GCCTGTCCTG CGAAGTGTGT AAATTCAAGG CTCACAAACG CTGTGCAGTG
    AGGGCAATGA ACAACTGCAA GTGGACGACC CTGGCCTCCA TCGGGAAGGA CATCATCGAG
    AACGAGGACG GGGTGGCTAT GCCTCACCAG TGGCTCGAGG GTAACTTGCC CGTGAGCGCC
    AAGTGTGCCG TCTGCGACAA AACGTGCGGC AGTGTTCTCC GTCTTCAAGA TTGGAGATGC
    CTTTGGTGTA GAACGATGGT ACACACTGCC TGTAAAGATT TATATCATCC TGTATGTCCA
    CTTGGCCAAT GTAAAGTATC TGTCATACCT CCAATTGCAC TCAACAGCAC TGATTCTGAT
    GGTTTCTGTA GAGCAACATT TTCATTCTGT GTTAGTCCTT TATTGGTTTT TGTCAATTCT
    AAGAGTGGAG ATAATCAGGG AGTAAAGTTT CTTCGTCGAT TTAAACAGTT ACTAAATCCA
    GCTCAGGTGT TTGATTTGAT GAATGGCGGT CCTCATTTGG GTTTAAGATT ATTCCAGAAG
    TTTGACAATT TCCGGATTCT TGTTTGTGGA GGAGATGGAA GTGTAGGTTG GGTTTTGTCA
    GAAATTGATA AGCTCAACTT GAACAAACAG TGTCAGCTGG GAGTACTGCC TCTGGGGACA
    GGAAACGACC TTGCCCGGGT TCTCGGCTGG GGAGGCTCGT ACGATGACGA CACCCAACTT
    CCTCAGATAC TTGAGAAGCT GGAACGAGCC AGTACCAAAA TGTTGGACAG GTGGAGTATT
    ATGACCTATG AACTCAAATT GCCGCCAAAA GCTTCTCTAC TTCCCGAACC TCCAGAGGCG
    TCTGAAGAAT TTTATATGAC AATTTATGAA GACTCAGTTG CAGCTCATCT TACAAAAATC
    CTCAATTCTG ATGAACATGC AGTGGTCATA TCTTCTACCA AGATACTCTG TGAGACTGTA
    AAGGACTTCG TGGCCAAAGT AGAAAAAGCA CACGAGAAAA CGCTGGAAAA AGCTGTTGCA
    GCTGATGCTG TGGCCAATAA ATGTTCCATC CTAAATGAGA AGCTTGAACA ACTGCTCCAG
    GCTTTGCACA TAGAGTCCCA GGCAGGTCCA GTTCTCCCAG GCGTCAGCCC TCTCAGGGTA
    GAAGATGCTG TGGAATCTTC CAGCGAAGAA TCCTTGTGTG AGAGCAAGGA ACATCTCTTG
    GATGACACTA CGAAACCCTC TTCCCAGAAA GCTGTCAAAC CAAGGGAAAT AATGCTGCGG
    GCAAACAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AGGCCAGAAA AGTGATGGAT
    GACCAGCCAG TTCACTCCTC TGAACTAGTT AATCAGTCAT TCGATTATGA TAGCACAGAA
    ACAGATGATT CCAAAGATGA GTTAAAAGAA GATGATGCGA AAGAGTCATT AACTGTTAAA
    GGTGCACCTC GGTCTCCGGA TGGCCGGGCT GGTCGTTCCC AAACTGACTC TGGCCCTGGT
    GCCCCTGTGG CAGCCAGCAA GGAAAACCTC CCTGTGCTCA ATACCAGAAT AATTTGCCCA
    GGTTTAAGAG CAGGACTAGC TGCCTCAATT GCTGGAAGTT CTATTATCAA CAAAATGTTG
    CTAGCAAATA TTGACCCTTT TGGTGCCACG CCATTTATTG ACCCAGATCC AGATTCAGTA
    GATGGCTATT CAGAAAAATG TGTCATGAAC AATTACTTTG GGATTGGATT AGATGCAAAA
    ATTTCATTAG AATTTAATAA TAAGAGAGAG GAGCACCCTG AAAAATGCAG GAGCCGAACT
    AAAAACTTGA TGTGGTATGG AGTCCTTGGA ACTCGGGAAT TATTACAAAG ATCATACAAG
    AATTTGGAAC AGAGGGTTCA ACTCGAATGT GACGGGCAGT ATATTCCTCT CCCCAGTCTG
    CAAGGAATAG CAGTGCTGAA CATCCCCAGC TATGCCGGAG GCACTAACTT TTGGGGTGGA
    ACTAAAGAAG ATGATATATT TGCTGCACCA TCATTTGATG ACAAGATCCT AGAAGTCGTT
    GCAATATTTG ACAGCGTGCA AATGGCCGTT TCAAGGGTCA TTAAACTGCA GCACCATCGA
    ATCGCCCAGT GCCGTACAGT AAAGATCACT ATCTTTGGTG ACGAGGGAGT CCCAGTGCAG
    GTGGACGGAG AAGCATGGGT TCAGCCTCCA GGGATTATCA AGATCGTGCA CAAGAACAGA
    GCTCAGATGC TCACCAGGGA CAGGGCCTTT GAAAGCACCC TGAAGTCTTG GGAAGATAAG
    CAGAAATGTG ATTCGGGTAA GCCAGTTCTC CGAACGCACT TGTACATCCA GCACGCGGCG
    GACCTGGCCA CGGAGGAAGT GTCTCAGATG CAGCTGTGCT CGCAGGCGGC CGAGGAGCTC
    ATCACCAGGA TCTGCGATGC GGCCACCATC CACTGTCTAT TGGAGCAGGA GCTGGCCCAC
    GCGGTGAACG CCTGCTCCCA CGCCCTGAAC AAAGCCAACC CACGCTTCCC AGAGAGTCTT
    ACAAGAGACA CTGCCACTGA AATAGCCATC AATGTGAAGG CCCTATATAA CGAAACAGAA
    TCTTTACTAG TGGGCAGGGT TCCTTTGCAA TTGGAATCTC CACATGAAGA GCGAGTATCC
    AATGCCTTAC ATTCCGTGGA AGTGGAGCTA CAGAAGTTAA CAGAGATTCC ATGGCTTTAT
    TATATCTTAC ACCCGAATGA GGATGAGGAG CCCCCCATGG ATTGCACCAA GAGAAATAGC
    AGAAGCACGG TATTTCGTAT TGTGCCCAAA TTCAAAAAGG AGAAGGTTCA GAAGCAGAAG
    ACAAGTTCAC AGCCTGTTCA GAAATGGGGC ACAGAGGAAG TTGCTGCTTG GCTGGATCTG
    CTCAATTTGG GAGAGTACAA AGAAATCTTC ATCCGCCATG ACATCAGAGG GGCTGAACTT
    TTGCATCTGG AAAGGCGAGA TCTTAAGGAC CTGGGGATAC CGAAAGTGGG TCATGTGAAG
    CGAATTCTCC AGGGAATTAA AGAGCTTGAA AGGAGCACCC TCCAGCCTGA GGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221231
    Clone ID Related Accession (Same CDS sequence) XM_025000022.1
    Accession Version XM_025000022.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3645bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCGGGG CCGGCAGCCA ACACCACCAC CCGGGCGCGG CGGGAGGAGC GGCAGGCGCT 
    GCGGTCACCC CCGCCACTGT CCCGGCGGGG CCCGGAGACG ACTCGTCAGA CAGCGAAGCG
    GAGCAGGAGG GACCCCAGAA GCTGATCCGC AAAGTGTCCA CCTCGGGGCA GATCCGCACC
    AAGACCAGTA TTAAAGAGGG ACAGCTGCTG AAGCAAACCA GTTCCTTCCA AAGGTGGAAA
    AAGCGATACT TCAAACTTCG AGGTCGTACA CTTTATTATG CAAAGGACTC AAAGTCTCTG
    ATATTTGATG AAGTTGACCT CTCAGATGCC AGTGTAGCTG AAGCAAGCAC GAAAAACGCT
    AACAACAGCT TCACGATAAT CACTCCATTT AGAAGACTAA TGCTGTGCGC TGAGAACAGG
    AAAGAGATGG AGGATTGGAT CAGCTCGCTG AAGTCTGTAC AGACCAGAGA ACCTTACGAG
    GTGGCCCAGT TTAACGTGGA ACATTTCTCA GGGATGCATA ACTGGTACGC GTGCTCCCAC
    GCCCGCCCCA CTTTCTGTAA CGTGTGCCGA GAGAGTCTTT CTGGAGTCAC CTCCCACGGC
    CTGTCCTGCG AAGTGTGTAA ATTCAAGGCT CACAAACGCT GTGCAGTGAG GGCAATGAAC
    AACTGCAAGT GGACGACCCT GGCCTCCATC GGGAAGGACA TCATCGAGAA CGAGGACGGG
    GTGGCTATGC CTCACCAGTG GCTCGAGGGT AACTTGCCCG TGAGCGCCAA GTGTGCCGTC
    TGCGACAAAA CGTGCGGCAG TGTTCTCCGT CTTCAAGATT GGAGATGCCT TTGGTGTAGA
    ACGATGGTAC ACACTGCCTG TAAAGATTTA TATCATCCTG TATGTCCACT TGGCCAATGT
    AAAGTATCTG TCATACCTCC AATTGCACTC AACAGCACTG ATTCTGATGG TTTCTGTAGA
    GCAACATTTT CATTCTGTGT TAGTCCTTTA TTGGTTTTTG TCAATTCTAA GAGTGGAGAT
    AATCAGGGAG TAAAGTTTCT TCGTCGATTT AAACAGTTAC TAAATCCAGC TCAGGTGTTT
    GATTTGATGA ATGGCGGTCC TCATTTGGGT TTAAGATTAT TCCAGAAGTT TGACAATTTC
    CGGATTCTTG TTTGTGGAGG AGATGGAAGT GTAGGTTGGG TTTTGTCAGA AATTGATAAG
    CTCAACTTGA ACAAACAGTG TCAGCTGGGA GTACTGCCTC TGGGGACAGG AAACGACCTT
    GCCCGGGTTC TCGGCTGGGG AGGCTCGTAC GATGACGACA CCCAACTTCC TCAGATACTT
    GAGAAGCTGG AACGAGCCAG TACCAAAATG TTGGACAGGT GGAGTATTAT GACCTATGAA
    CTCAAATTGC CGCCAAAAGC TTCTCTACTT CCCGAACCTC CAGAGGCGTC TGAAGAATTT
    TATATGACAA TTTATGAAGA CTCAGTTGCA GCTCATCTTA CAAAAATCCT CAATTCTGAT
    GAACATGCAG TGGTCATATC TTCTACCAAG ATACTCTGTG AGACTGTAAA GGACTTCGTG
    GCCAAAGTAG AAAAAGCACA CGAGAAAACG CTGGAAAAAG CTGTTGCAGC TGATGCTGTG
    GCCAATAAAT GTTCCATCCT AAATGAGAAG CTTGAACAAC TGCTCCAGGC TTTGCACATA
    GAGTCCCAGG CAGGTCCAGT TCTCCCAGGC GTCAGCCCTC TCAGGGTAGA AGATGCTGTG
    GAATCTTCCA GCGAAGAATC CTTGTGTGAG AGCAAGGAAC ATCTCTTGGA TGACACTACG
    AAACCCTCTT CCCAGAAAGC TGTCAAACCA AGGGAAATAA TGCTGCGGGC AAACAGTTTA
    AAGAAAGCAG TGAGGCAAGT CATTGAGGAG GCCAGAAAAG TGATGGATGA CCAGCCAGTT
    CACTCCTCTG AACTAGTTAA TCAGTCATTC GATTATGATA GCACAGAAAC AGATGATTCC
    AAAGATGAGT TAAAAGAAGA TGATGCGAAA GAGTCATTAA CTGTTAAAGG TGCACCTCGG
    TCTCCGGATG GCCGGGCTGG TCGTTCCCAA ACTGACTCTG GCCCTGGTGC CCCTGTGGCA
    GCCAGCAAGG AAAACCTCCC TGTGCTCAAT ACCAGAATAA TTTGCCCAGG TTTAAGAGCA
    GGACTAGCTG CCTCAATTGC TGGAAGTTCT ATTATCAACA AAATGTTGCT AGCAAATATT
    GACCCTTTTG GTGCCACGCC ATTTATTGAC CCAGATCCAG ATTCAGTAGA TGGCTATTCA
    GAAAAATGTG TCATGAACAA TTACTTTGGG ATTGGATTAG ATGCAAAAAT TTCATTAGAA
    TTTAATAATA AGAGAGAGGA GCACCCTGAA AAATGCAGGA GCCGAACTAA AAACTTGATG
    TGGTATGGAG TCCTTGGAAC TCGGGAATTA TTACAAAGAT CATACAAGAA TTTGGAACAG
    AGGGTTCAAC TCGAATGTGA CGGGCAGTAT ATTCCTCTCC CCAGTCTGCA AGGAATAGCA
    GTGCTGAACA TCCCCAGCTA TGCCGGAGGC ACTAACTTTT GGGGTGGAAC TAAAGAAGAT
    GATATATTTG CTGCACCATC ATTTGATGAC AAGATCCTAG AAGTCGTTGC AATATTTGAC
    AGCGTGCAAA TGGCCGTTTC AAGGGTCATT AAACTGCAGC ACCATCGAAT CGCCCAGTGC
    CGTACAGTAA AGATCACTAT CTTTGGTGAC GAGGGAGTCC CAGTGCAGGT GGACGGAGAA
    GCATGGGTTC AGCCTCCAGG GATTATCAAG ATCGTGCACA AGAACAGAGC TCAGATGCTC
    ACCAGGGACA GGGCCTTTGA AAGCACCCTG AAGTCTTGGG AAGATAAGCA GAAATGTGAT
    TCGGGTAAGC CAGTTCTCCG AACGCACTTG TACATCCAGC ACGCGGCGGA CCTGGCCACG
    GAGGAAGTGT CTCAGATGCA GCTGTGCTCG CAGGCGGCCG AGGAGCTCAT CACCAGGATC
    TGCGATGCGG CCACCATCCA CTGTCTATTG GAGCAGGAGC TGGCCCACGC GGTGAACGCC
    TGCTCCCACG CCCTGAACAA AGCCAACCCA CGCTTCCCAG AGAGTCTTAC AAGAGACACT
    GCCACTGAAA TAGCCATCAA TGTGAAGGCC CTATATAACG AAACAGAATC TTTACTAGTG
    GGCAGGGTTC CTTTGCAATT GGAATCTCCA CATGAAGAGC GAGTATCCAA TGCCTTACAT
    TCCGTGGAAG TGGAGCTACA GAAGTTAACA GAGATTCCAT GGCTTTATTA TATCTTACAC
    CCGAATGAGG ATGAGGAGCC CCCCATGGAT TGCACCAAGA GAAATAGCAG AAGCACGGTA
    TTTCGTATTG TGCCCAAATT CAAAAAGGAG AAGGTTCAGA AGCAGAAGAC AAGTTCACAG
    CCTGTTCAGA AATGGGGCAC AGAGGAAGTT GCTGCTTGGC TGGATCTGCT CAATTTGGGA
    GAGTACAAAG AAATCTTCAT CCGCCATGAC ATCAGAGGGG CTGAACTTTT GCATCTGGAA
    AGGCGAGATC TTAAGGACCT GGGGATACCG AAAGTGGGTC ATGTGAAGCG AATTCTCCAG
    GGAATTAAAG AGCTTGAAAG GAGCACCCTC CAGCCTGAGG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855790.1
    CDS2877..6521
    Translation

    Target ORF information:

    RefSeq Version XM_025000022.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000022.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCGGGG CCGGCAGCCA ACACCACCAC CCGGGCGCGG CGGGAGGAGC GGCAGGCGCT 
    GCGGTCACCC CCGCCACTGT CCCGGCGGGG CCCGGAGACG ACTCGTCAGA CAGCGAAGCG
    GAGCAGGAGG GACCCCAGAA GCTGATCCGC AAAGTGTCCA CCTCGGGGCA GATCCGCACC
    AAGACCAGTA TTAAAGAGGG ACAGCTGCTG AAGCAAACCA GTTCCTTCCA AAGGTGGAAA
    AAGCGATACT TCAAACTTCG AGGTCGTACA CTTTATTATG CAAAGGACTC AAAGTCTCTG
    ATATTTGATG AAGTTGACCT CTCAGATGCC AGTGTAGCTG AAGCAAGCAC GAAAAACGCT
    AACAACAGCT TCACGATAAT CACTCCATTT AGAAGACTAA TGCTGTGCGC TGAGAACAGG
    AAAGAGATGG AGGATTGGAT CAGCTCGCTG AAGTCTGTAC AGACCAGAGA ACCTTACGAG
    GTGGCCCAGT TTAACGTGGA ACATTTCTCA GGGATGCATA ACTGGTACGC GTGCTCCCAC
    GCCCGCCCCA CTTTCTGTAA CGTGTGCCGA GAGAGTCTTT CTGGAGTCAC CTCCCACGGC
    CTGTCCTGCG AAGTGTGTAA ATTCAAGGCT CACAAACGCT GTGCAGTGAG GGCAATGAAC
    AACTGCAAGT GGACGACCCT GGCCTCCATC GGGAAGGACA TCATCGAGAA CGAGGACGGG
    GTGGCTATGC CTCACCAGTG GCTCGAGGGT AACTTGCCCG TGAGCGCCAA GTGTGCCGTC
    TGCGACAAAA CGTGCGGCAG TGTTCTCCGT CTTCAAGATT GGAGATGCCT TTGGTGTAGA
    ACGATGGTAC ACACTGCCTG TAAAGATTTA TATCATCCTG TATGTCCACT TGGCCAATGT
    AAAGTATCTG TCATACCTCC AATTGCACTC AACAGCACTG ATTCTGATGG TTTCTGTAGA
    GCAACATTTT CATTCTGTGT TAGTCCTTTA TTGGTTTTTG TCAATTCTAA GAGTGGAGAT
    AATCAGGGAG TAAAGTTTCT TCGTCGATTT AAACAGTTAC TAAATCCAGC TCAGGTGTTT
    GATTTGATGA ATGGCGGTCC TCATTTGGGT TTAAGATTAT TCCAGAAGTT TGACAATTTC
    CGGATTCTTG TTTGTGGAGG AGATGGAAGT GTAGGTTGGG TTTTGTCAGA AATTGATAAG
    CTCAACTTGA ACAAACAGTG TCAGCTGGGA GTACTGCCTC TGGGGACAGG AAACGACCTT
    GCCCGGGTTC TCGGCTGGGG AGGCTCGTAC GATGACGACA CCCAACTTCC TCAGATACTT
    GAGAAGCTGG AACGAGCCAG TACCAAAATG TTGGACAGGT GGAGTATTAT GACCTATGAA
    CTCAAATTGC CGCCAAAAGC TTCTCTACTT CCCGAACCTC CAGAGGCGTC TGAAGAATTT
    TATATGACAA TTTATGAAGA CTCAGTTGCA GCTCATCTTA CAAAAATCCT CAATTCTGAT
    GAACATGCAG TGGTCATATC TTCTACCAAG ATACTCTGTG AGACTGTAAA GGACTTCGTG
    GCCAAAGTAG AAAAAGCACA CGAGAAAACG CTGGAAAAAG CTGTTGCAGC TGATGCTGTG
    GCCAATAAAT GTTCCATCCT AAATGAGAAG CTTGAACAAC TGCTCCAGGC TTTGCACATA
    GAGTCCCAGG CAGGTCCAGT TCTCCCAGGC GTCAGCCCTC TCAGGGTAGA AGATGCTGTG
    GAATCTTCCA GCGAAGAATC CTTGTGTGAG AGCAAGGAAC ATCTCTTGGA TGACACTACG
    AAACCCTCTT CCCAGAAAGC TGTCAAACCA AGGGAAATAA TGCTGCGGGC AAACAGTTTA
    AAGAAAGCAG TGAGGCAAGT CATTGAGGAG GCCAGAAAAG TGATGGATGA CCAGCCAGTT
    CACTCCTCTG AACTAGTTAA TCAGTCATTC GATTATGATA GCACAGAAAC AGATGATTCC
    AAAGATGAGT TAAAAGAAGA TGATGCGAAA GAGTCATTAA CTGTTAAAGG TGCACCTCGG
    TCTCCGGATG GCCGGGCTGG TCGTTCCCAA ACTGACTCTG GCCCTGGTGC CCCTGTGGCA
    GCCAGCAAGG AAAACCTCCC TGTGCTCAAT ACCAGAATAA TTTGCCCAGG TTTAAGAGCA
    GGACTAGCTG CCTCAATTGC TGGAAGTTCT ATTATCAACA AAATGTTGCT AGCAAATATT
    GACCCTTTTG GTGCCACGCC ATTTATTGAC CCAGATCCAG ATTCAGTAGA TGGCTATTCA
    GAAAAATGTG TCATGAACAA TTACTTTGGG ATTGGATTAG ATGCAAAAAT TTCATTAGAA
    TTTAATAATA AGAGAGAGGA GCACCCTGAA AAATGCAGGA GCCGAACTAA AAACTTGATG
    TGGTATGGAG TCCTTGGAAC TCGGGAATTA TTACAAAGAT CATACAAGAA TTTGGAACAG
    AGGGTTCAAC TCGAATGTGA CGGGCAGTAT ATTCCTCTCC CCAGTCTGCA AGGAATAGCA
    GTGCTGAACA TCCCCAGCTA TGCCGGAGGC ACTAACTTTT GGGGTGGAAC TAAAGAAGAT
    GATATATTTG CTGCACCATC ATTTGATGAC AAGATCCTAG AAGTCGTTGC AATATTTGAC
    AGCGTGCAAA TGGCCGTTTC AAGGGTCATT AAACTGCAGC ACCATCGAAT CGCCCAGTGC
    CGTACAGTAA AGATCACTAT CTTTGGTGAC GAGGGAGTCC CAGTGCAGGT GGACGGAGAA
    GCATGGGTTC AGCCTCCAGG GATTATCAAG ATCGTGCACA AGAACAGAGC TCAGATGCTC
    ACCAGGGACA GGGCCTTTGA AAGCACCCTG AAGTCTTGGG AAGATAAGCA GAAATGTGAT
    TCGGGTAAGC CAGTTCTCCG AACGCACTTG TACATCCAGC ACGCGGCGGA CCTGGCCACG
    GAGGAAGTGT CTCAGATGCA GCTGTGCTCG CAGGCGGCCG AGGAGCTCAT CACCAGGATC
    TGCGATGCGG CCACCATCCA CTGTCTATTG GAGCAGGAGC TGGCCCACGC GGTGAACGCC
    TGCTCCCACG CCCTGAACAA AGCCAACCCA CGCTTCCCAG AGAGTCTTAC AAGAGACACT
    GCCACTGAAA TAGCCATCAA TGTGAAGGCC CTATATAACG AAACAGAATC TTTACTAGTG
    GGCAGGGTTC CTTTGCAATT GGAATCTCCA CATGAAGAGC GAGTATCCAA TGCCTTACAT
    TCCGTGGAAG TGGAGCTACA GAAGTTAACA GAGATTCCAT GGCTTTATTA TATCTTACAC
    CCGAATGAGG ATGAGGAGCC CCCCATGGAT TGCACCAAGA GAAATAGCAG AAGCACGGTA
    TTTCGTATTG TGCCCAAATT CAAAAAGGAG AAGGTTCAGA AGCAGAAGAC AAGTTCACAG
    CCTGTTCAGA AATGGGGCAC AGAGGAAGTT GCTGCTTGGC TGGATCTGCT CAATTTGGGA
    GAGTACAAAG AAATCTTCAT CCGCCATGAC ATCAGAGGGG CTGAACTTTT GCATCTGGAA
    AGGCGAGATC TTAAGGACCT GGGGATACCG AAAGTGGGTC ATGTGAAGCG AATTCTCCAG
    GGAATTAAAG AGCTTGAAAG GAGCACCCTC CAGCCTGAGG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221232
    Clone ID Related Accession (Same CDS sequence) XM_025000023.1
    Accession Version XM_025000023.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGCCGC GGCACGTGCG CCCCTCGCCG GGCGCCCGCA GCCCGACCTG GGAGGAACCG 
    GAGTCGGACA GGATGGCCGG GGCCGGCAGC CAACACCACC ACCCGGGCGC GGCGGGAGGA
    GCGGCAGGCG CTGCGGTCAC CCCCGCCACT GTCCCGGCGG GGCCCGGAGA CGACTCGTCA
    GACAGCGAAG CGGAGCAGGA GGGACCCCAG AAGCTGATCC GCAAAGTGTC CACCTCGGGG
    CAGATCCGCA CCAAGACCAG TATTAAAGAG GGACAGCTGC TGAAGCAAAC CAGTTCCTTC
    CAAAGGTGGA AAAAGCGATA CTTCAAACTT CGAGGTCGTA CACTTTATTA TGCAAAGGAC
    TCAAAGTCTC TGATATTTGA TGAAGTTGAC CTCTCAGATG CCAGTGTAGC TGAAGCAAGC
    ACGAAAAACG CTAACAACAG CTTCACGATA ATCACTCCAT TTAGAAGACT AATGCTGTGC
    GCTGAGAACA GGAAAGAGAT GGAGGATTGG ATCAGCTCGC TGAAGTCTGT ACAGACCAGA
    GAACCTTACG AGGTGGCCCA GTTTAACGTG GAACATTTCT CAGGGATGCA TAACTGGTAC
    GCGTGCTCCC ACGCCCGCCC CACTTTCTGT AACGTGTGCC GAGAGAGTCT TTCTGGAGTC
    ACCTCCCACG GCCTGTCCTG CGAAGTGTGT AAATTCAAGG CTCACAAACG CTGTGCAGTG
    AGGGCAATGA ACAACTGCAA GTGGACGACC CTGGCCTCCA TCGGGAAGGA CATCATCGAG
    AACGAGGACG GGGTGGCTAT GCCTCACCAG TGGCTCGAGG GTAACTTGCC CGTGAGCGCC
    AAGTGTGCCG TCTGCGACAA AACGTGCGGC AGTGTTCTCC GTCTTCAAGA TTGGAGATGC
    CTTTGGTGTA GAACGATGGT ACACACTGCC TGTAAAGATT TATATCATCC TGTATGTCCA
    CTTGGCCAAT GTAAAGTATC TGTCATACCT CCAATTGCAC TCAACAGCAC TGATTCTGAT
    GGTTTCTGTA GAGCAACATT TTCATTCTGT GTTAGTCCTT TATTGGTTTT TGTCAATTCT
    AAGAGTGGAG ATAATCAGGG AGTAAAGTTT CTTCGTCGAT TTAAACAGTT ACTAAATCCA
    GCTCAGGTGT TTGATTTGAT GAATGGCGGT CCTCATTTGG GTTTAAGATT ATTCCAGAAG
    TTTGACAATT TCCGGATTCT TGTTTGTGGA GGAGATGGAA GTGTAGGTTG GGTTTTGTCA
    GAAATTGATA AGCTCAACTT GAACAAACAG TGTCAGCTGG GAGTACTGCC TCTGGGGACA
    GGAAACGACC TTGCCCGGGT TCTCGGCTGG GGAGGCTCGT ACGATGACGA CACCCAACTT
    CCTCAGATAC TTGAGAAGCT GGAACGAGCC AGTACCAAAA TGTTGGACAG GTGGAGTATT
    ATGACCTATG AACTCAAATT GCCGCCAAAA GCTTCTCTAC TTCCCGAACC TCCAGAGGCG
    TCTGAAGAAT TTTATATGAC AATTTATGAA GACTCAGTTG CAGCTCATCT TACAAAAATC
    CTCAATTCTG ATGAACATGC AGTGGTCATA TCTTCTACCA AGATACTCTG TGAGACTGTA
    AAGGACTTCG TGGCCAAAGT AGAAAAAGCA CACGAGAAAA CGCTGGAAAA AGCTGTTGCA
    GCTGATGCTG TGGCCAATAA ATGTTCCATC CTAAATGAGA AGCTTGAACA ACTGCTCCAG
    GCTTTGCACA TAGAGTCCCA GGCAGGTCCA GTTCTCCCAG GCGTCAGCCC TCTCAGGGTA
    GAAGATGCTG TGGAATCTTC CAGCGAAGAA TCCTTGTGTG AGAGCAAGGA ACATCTCTTG
    GATGACACTA CGAAACCCTC TTCCCAGAAA GCTGTCAAAC CAAGGGAAAT AATGCTGCGG
    GCAAACAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AGGCCAGAAA AGTGATGGAT
    GACCAGCCAG TTCACTCCTC TGAACTAGTT AATCAGTCAT TCGATTATGA TAGCACAGAA
    ACAGATGATT CCAAAGATGA GTTAAAAGAA GATGATGCGA AAGAGTCATT AACTGGTTTA
    AGAGCAGGAC TAGCTGCCTC AATTGCTGGA AGTTCTATTA TCAACAAAAT GTTGCTAGCA
    AATATTGACC CTTTTGGTGC CACGCCATTT ATTGACCCAG ATCCAGATTC AGTAGATGGC
    TATTCAGAAA AATGTGTCAT GAACAATTAC TTTGGGATTG GATTAGATGC AAAAATTTCA
    TTAGAATTTA ATAATAAGAG AGAGGAGCAC CCTGAAAAAT GCAGGAGCCG AACTAAAAAC
    TTGATGTGGT ATGGAGTCCT TGGAACTCGG GAATTATTAC AAAGATCATA CAAGAATTTG
    GAACAGAGGG TTCAACTCGA ATGTGACGGG CAGTATATTC CTCTCCCCAG TCTGCAAGGA
    ATAGCAGTGC TGAACATCCC CAGCTATGCC GGAGGCACTA ACTTTTGGGG TGGAACTAAA
    GAAGATGATA TATTTGCTGC ACCATCATTT GATGACAAGA TCCTAGAAGT CGTTGCAATA
    TTTGACAGCG TGCAAATGGC CGTTTCAAGG GTCATTAAAC TGCAGCACCA TCGAATCGCC
    CAGTGCCGTA CAGTAAAGAT CACTATCTTT GGTGACGAGG GAGTCCCAGT GCAGGTGGAC
    GGAGAAGCAT GGGTTCAGCC TCCAGGGATT ATCAAGATCG TGCACAAGAA CAGAGCTCAG
    ATGCTCACCA GGGACAGGGC CTTTGAAAGC ACCCTGAAGT CTTGGGAAGA TAAGCAGAAA
    TGTGATTCGG GTAAGCCAGT TCTCCGAACG CACTTGTACA TCCAGCACGC GGCGGACCTG
    GCCACGGAGG AAGTGTCTCA GATGCAGCTG TGCTCGCAGG CGGCCGAGGA GCTCATCACC
    AGGATCTGCG ATGCGGCCAC CATCCACTGT CTATTGGAGC AGGAGCTGGC CCACGCGGTG
    AACGCCTGCT CCCACGCCCT GAACAAAGCC AACCCACGCT TCCCAGAGAG TCTTACAAGA
    GACACTGCCA CTGAAATAGC CATCAATGTG AAGGCCCTAT ATAACGAAAC AGAATCTTTA
    CTAGTGGGCA GGGTTCCTTT GCAATTGGAA TCTCCACATG AAGAGCGAGT ATCCAATGCC
    TTACATTCCG TGGAAGTGGA GCTACAGAAG TTAACAGAGA TTCCATGGCT TTATTATATC
    TTACACCCGA ATGAGGATGA GGAGCCCCCC ATGGATTGCA CCAAGAGAAA TAGCAGAAGC
    ACGGTATTTC GTATTGTGCC CAAATTCAAA AAGGAGAAGG TTCAGAAGCA GAAGACAAGT
    TCACAGCCTG TTCAGAAATG GGGCACAGAG GAAGTTGCTG CTTGGCTGGA TCTGCTCAAT
    TTGGGAGAGT ACAAAGAAAT CTTCATCCGC CATGACATCA GAGGGGCTGA ACTTTTGCAT
    CTGGAAAGGC GAGATCTTAA GGACCTGGGG ATACCGAAAG TGGGTCATGT GAAGCGAATT
    CTCCAGGGAA TTAAAGAGCT TGAAAGGAGC ACCCTCCAGC CTGAGGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855791.1
    CDS36..3626
    Translation

    Target ORF information:

    RefSeq Version XM_025000023.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000023.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGCCGC GGCACGTGCG CCCCTCGCCG GGCGCCCGCA GCCCGACCTG GGAGGAACCG 
    GAGTCGGACA GGATGGCCGG GGCCGGCAGC CAACACCACC ACCCGGGCGC GGCGGGAGGA
    GCGGCAGGCG CTGCGGTCAC CCCCGCCACT GTCCCGGCGG GGCCCGGAGA CGACTCGTCA
    GACAGCGAAG CGGAGCAGGA GGGACCCCAG AAGCTGATCC GCAAAGTGTC CACCTCGGGG
    CAGATCCGCA CCAAGACCAG TATTAAAGAG GGACAGCTGC TGAAGCAAAC CAGTTCCTTC
    CAAAGGTGGA AAAAGCGATA CTTCAAACTT CGAGGTCGTA CACTTTATTA TGCAAAGGAC
    TCAAAGTCTC TGATATTTGA TGAAGTTGAC CTCTCAGATG CCAGTGTAGC TGAAGCAAGC
    ACGAAAAACG CTAACAACAG CTTCACGATA ATCACTCCAT TTAGAAGACT AATGCTGTGC
    GCTGAGAACA GGAAAGAGAT GGAGGATTGG ATCAGCTCGC TGAAGTCTGT ACAGACCAGA
    GAACCTTACG AGGTGGCCCA GTTTAACGTG GAACATTTCT CAGGGATGCA TAACTGGTAC
    GCGTGCTCCC ACGCCCGCCC CACTTTCTGT AACGTGTGCC GAGAGAGTCT TTCTGGAGTC
    ACCTCCCACG GCCTGTCCTG CGAAGTGTGT AAATTCAAGG CTCACAAACG CTGTGCAGTG
    AGGGCAATGA ACAACTGCAA GTGGACGACC CTGGCCTCCA TCGGGAAGGA CATCATCGAG
    AACGAGGACG GGGTGGCTAT GCCTCACCAG TGGCTCGAGG GTAACTTGCC CGTGAGCGCC
    AAGTGTGCCG TCTGCGACAA AACGTGCGGC AGTGTTCTCC GTCTTCAAGA TTGGAGATGC
    CTTTGGTGTA GAACGATGGT ACACACTGCC TGTAAAGATT TATATCATCC TGTATGTCCA
    CTTGGCCAAT GTAAAGTATC TGTCATACCT CCAATTGCAC TCAACAGCAC TGATTCTGAT
    GGTTTCTGTA GAGCAACATT TTCATTCTGT GTTAGTCCTT TATTGGTTTT TGTCAATTCT
    AAGAGTGGAG ATAATCAGGG AGTAAAGTTT CTTCGTCGAT TTAAACAGTT ACTAAATCCA
    GCTCAGGTGT TTGATTTGAT GAATGGCGGT CCTCATTTGG GTTTAAGATT ATTCCAGAAG
    TTTGACAATT TCCGGATTCT TGTTTGTGGA GGAGATGGAA GTGTAGGTTG GGTTTTGTCA
    GAAATTGATA AGCTCAACTT GAACAAACAG TGTCAGCTGG GAGTACTGCC TCTGGGGACA
    GGAAACGACC TTGCCCGGGT TCTCGGCTGG GGAGGCTCGT ACGATGACGA CACCCAACTT
    CCTCAGATAC TTGAGAAGCT GGAACGAGCC AGTACCAAAA TGTTGGACAG GTGGAGTATT
    ATGACCTATG AACTCAAATT GCCGCCAAAA GCTTCTCTAC TTCCCGAACC TCCAGAGGCG
    TCTGAAGAAT TTTATATGAC AATTTATGAA GACTCAGTTG CAGCTCATCT TACAAAAATC
    CTCAATTCTG ATGAACATGC AGTGGTCATA TCTTCTACCA AGATACTCTG TGAGACTGTA
    AAGGACTTCG TGGCCAAAGT AGAAAAAGCA CACGAGAAAA CGCTGGAAAA AGCTGTTGCA
    GCTGATGCTG TGGCCAATAA ATGTTCCATC CTAAATGAGA AGCTTGAACA ACTGCTCCAG
    GCTTTGCACA TAGAGTCCCA GGCAGGTCCA GTTCTCCCAG GCGTCAGCCC TCTCAGGGTA
    GAAGATGCTG TGGAATCTTC CAGCGAAGAA TCCTTGTGTG AGAGCAAGGA ACATCTCTTG
    GATGACACTA CGAAACCCTC TTCCCAGAAA GCTGTCAAAC CAAGGGAAAT AATGCTGCGG
    GCAAACAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AGGCCAGAAA AGTGATGGAT
    GACCAGCCAG TTCACTCCTC TGAACTAGTT AATCAGTCAT TCGATTATGA TAGCACAGAA
    ACAGATGATT CCAAAGATGA GTTAAAAGAA GATGATGCGA AAGAGTCATT AACTGGTTTA
    AGAGCAGGAC TAGCTGCCTC AATTGCTGGA AGTTCTATTA TCAACAAAAT GTTGCTAGCA
    AATATTGACC CTTTTGGTGC CACGCCATTT ATTGACCCAG ATCCAGATTC AGTAGATGGC
    TATTCAGAAA AATGTGTCAT GAACAATTAC TTTGGGATTG GATTAGATGC AAAAATTTCA
    TTAGAATTTA ATAATAAGAG AGAGGAGCAC CCTGAAAAAT GCAGGAGCCG AACTAAAAAC
    TTGATGTGGT ATGGAGTCCT TGGAACTCGG GAATTATTAC AAAGATCATA CAAGAATTTG
    GAACAGAGGG TTCAACTCGA ATGTGACGGG CAGTATATTC CTCTCCCCAG TCTGCAAGGA
    ATAGCAGTGC TGAACATCCC CAGCTATGCC GGAGGCACTA ACTTTTGGGG TGGAACTAAA
    GAAGATGATA TATTTGCTGC ACCATCATTT GATGACAAGA TCCTAGAAGT CGTTGCAATA
    TTTGACAGCG TGCAAATGGC CGTTTCAAGG GTCATTAAAC TGCAGCACCA TCGAATCGCC
    CAGTGCCGTA CAGTAAAGAT CACTATCTTT GGTGACGAGG GAGTCCCAGT GCAGGTGGAC
    GGAGAAGCAT GGGTTCAGCC TCCAGGGATT ATCAAGATCG TGCACAAGAA CAGAGCTCAG
    ATGCTCACCA GGGACAGGGC CTTTGAAAGC ACCCTGAAGT CTTGGGAAGA TAAGCAGAAA
    TGTGATTCGG GTAAGCCAGT TCTCCGAACG CACTTGTACA TCCAGCACGC GGCGGACCTG
    GCCACGGAGG AAGTGTCTCA GATGCAGCTG TGCTCGCAGG CGGCCGAGGA GCTCATCACC
    AGGATCTGCG ATGCGGCCAC CATCCACTGT CTATTGGAGC AGGAGCTGGC CCACGCGGTG
    AACGCCTGCT CCCACGCCCT GAACAAAGCC AACCCACGCT TCCCAGAGAG TCTTACAAGA
    GACACTGCCA CTGAAATAGC CATCAATGTG AAGGCCCTAT ATAACGAAAC AGAATCTTTA
    CTAGTGGGCA GGGTTCCTTT GCAATTGGAA TCTCCACATG AAGAGCGAGT ATCCAATGCC
    TTACATTCCG TGGAAGTGGA GCTACAGAAG TTAACAGAGA TTCCATGGCT TTATTATATC
    TTACACCCGA ATGAGGATGA GGAGCCCCCC ATGGATTGCA CCAAGAGAAA TAGCAGAAGC
    ACGGTATTTC GTATTGTGCC CAAATTCAAA AAGGAGAAGG TTCAGAAGCA GAAGACAAGT
    TCACAGCCTG TTCAGAAATG GGGCACAGAG GAAGTTGCTG CTTGGCTGGA TCTGCTCAAT
    TTGGGAGAGT ACAAAGAAAT CTTCATCCGC CATGACATCA GAGGGGCTGA ACTTTTGCAT
    CTGGAAAGGC GAGATCTTAA GGACCTGGGG ATACCGAAAG TGGGTCATGT GAAGCGAATT
    CTCCAGGGAA TTAAAGAGCT TGAAAGGAGC ACCCTCCAGC CTGAGGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221233
    Clone ID Related Accession (Same CDS sequence) XM_025000024.1
    Accession Version XM_025000024.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGCCGC GGCACGTGCG CCCCTCGCCG GGCGCCCGCA GCCCGACCTG GGAGGAACCG 
    GAGTCGGACA GGATGGCCGG GGCCGGCAGC CAACACCACC ACCCGGGCGC GGCGGGAGGA
    GCGGCAGGCG CTGCGGTCAC CCCCGCCACT GTCCCGGCGG GGCCCGGAGA CGACTCGTCA
    GACAGCGAAG CGGAGCAGGA GGGACCCCAG AAGCTGATCC GCAAAGTGTC CACCTCGGGG
    CAGATCCGCA CCAAGACCAG TATTAAAGAG GGACAGCTGC TGAAGCAAAC CAGTTCCTTC
    CAAAGGTGGA AAAAGCGATA CTTCAAACTT CGAGGTCGTA CACTTTATTA TGCAAAGGAC
    TCAAAGTCTC TGATATTTGA TGAAGTTGAC CTCTCAGATG CCAGTGTAGC TGAAGCAAGC
    ACGAAAAACG CTAACAACAG CTTCACGATA ATCACTCCAT TTAGAAGACT AATGCTGTGC
    GCTGAGAACA GGAAAGAGAT GGAGGATTGG ATCAGCTCGC TGAAGTCTGT ACAGACCAGA
    GAACCTTACG AGGTGGCCCA GTTTAACGTG GAACATTTCT CAGGGATGCA TAACTGGTAC
    GCGTGCTCCC ACGCCCGCCC CACTTTCTGT AACGTGTGCC GAGAGAGTCT TTCTGGAGTC
    ACCTCCCACG GCCTGTCCTG CGAAGTGTGT AAATTCAAGG CTCACAAACG CTGTGCAGTG
    AGGGCAATGA ACAACTGCAA GTGGACGACC CTGGCCTCCA TCGGGAAGGA CATCATCGAG
    AACGAGGACG GGGTGGCTAT GCCTCACCAG TGGCTCGAGG GTAACTTGCC CGTGAGCGCC
    AAGTGTGCCG TCTGCGACAA AACGTGCGGC AGTGTTCTCC GTCTTCAAGA TTGGAGATGC
    CTTTGGTGTA GAACGATGGT ACACACTGCC TGTAAAGATT TATATCATCC TGTATGTCCA
    CTTGGCCAAT GTAAAGTATC TGTCATACCT CCAATTGCAC TCAACAGCAC TGATTCTGAT
    GGTTTCTGTA GAGCAACATT TTCATTCTGT GTTAGTCCTT TATTGGTTTT TGTCAATTCT
    AAGAGTGGAG ATAATCAGGG AGTAAAGTTT CTTCGTCGAT TTAAACAGTT ACTAAATCCA
    GCTCAGGTGT TTGATTTGAT GAATGGCGGT CCTCATTTGG GTTTAAGATT ATTCCAGAAG
    TTTGACAATT TCCGGATTCT TGTTTGTGGA GGAGATGGAA GTGTAGGTTG GGTTTTGTCA
    GAAATTGATA AGCTCAACTT GAACAAACAG TGTCAGCTGG GAGTACTGCC TCTGGGGACA
    GGAAACGACC TTGCCCGGGT TCTCGGCTGG GGAGGCTCGT ACGATGACGA CACCCAACTT
    CCTCAGATAC TTGAGAAGCT GGAACGAGCC AGTACCAAAA TGTTGGACAG GTGGAGTATT
    ATGACCTATG AACTCAAATT GCCGCCAAAA GCTTCTCTAC TTCCCGAACC TCCAGAGGCG
    TCTGAAGAAT TTTATATGAC AATTTATGAA GACTCAGTTG CAGCTCATCT TACAAAAATC
    CTCAATTCTG ATGAACATGC AGTGGTCATA TCTTCTACCA AGATACTCTG TGAGACTGTA
    AAGGACTTCG TGGCCAAAGT AGAAAAAGCA CACGAGAAAA CGCTGGAAAA AGCTGTTGCA
    GCTGATGCTG TGGCCAATAA ATGTTCCATC CTAAATGAGA AGCTTGAACA ACTGCTCCAG
    GCTTTGCACA TAGAGTCCCA GGCAGGTCCA GTTCTCCCAG GCGTCAGCCC TCTCAGGGTA
    GAAGATGCTG TGGAATCTTC CAGCGAAGAA TCCTTGTGTG AGAGCAAGGA ACATCTCTTG
    GATGACACTA CGAAACCCTC TTCCCAGAAA GCTGTCAAAC CAAGGGAAAT AATGCTGCGG
    GCAAACAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AGGCCAGAAA AGTGATGGAT
    GACCAGCCAG TTCACTCCTC TGAACTAGTT AATCAGTCAT TCGATTATGA TAGCACAGAA
    ACAGATGATT CCAAAGATGA GTTAAAAGAA GATGATGCGA AAGAGTCATT AACTGTTAAA
    GGTGCACCTC GGTCTCCGGA TGGCCGGGCT GGTCGTTCCC AAACTGACTC TGGCCCTGGT
    GCCCCTGTGG CAGCCAGCAA GGAAAACCTC CCTGTGCTCA ATACCAGAAT AATTTGCCCA
    GGTTTAAGAG CAGGACTAGC TGCCTCAATT GCTGGAAGTT CTATTATCAA CAAAATGTTG
    CTAGCAAATA TTGACCCTTT TGGTGCCACG CCATTTATTG ACCCAGATCC AGATTCAGTA
    GATGGCTATT CAGAAAAATG TGTCATGAAC AATTACTTTG GGATTGGATT AGATGCAAAA
    ATTTCATTAG AATTTAATAA TAAGAGAGAG GAGCACCCTG AAAAATGCAG GAGCCGAACT
    AAAAACTTGA TGTGGTATGG AGTCCTTGGA ACTCGGGAAT TATTACAAAG ATCATACAAG
    AATTTGGAAC AGAGGGTTCA ACTCGAATGT GACGGGCAGT ATATTCCTCT CCCCAGTCTG
    CAAGGAATAG CAGTGCTGAA CATCCCCAGC TATGCCGGAG GCACTAACTT TTGGGGTGGA
    ACTAAAGAAG ATGATATATT TGCTGCACCA TCATTTGATG ACAAGATCCT AGAAGTCGTT
    GCAATATTTG ACAGCGTGCA AATGGCCGTT TCAAGGGTCA TTAAACTGCA GCACCATCGA
    ATCGCCCAGT GCCGTACAGT AAAGATCACT ATCTTTGGTG ACGAGGGAGT CCCAGTGCAG
    GTGGACGGAG AAGCATGGGT TCAGCCTCCA GGGATTATCA AGATCGTGCA CAAGAACAGA
    GCTCAGATGC TCACCAGGGA CAGGGCCTTT GAAAGCACCC TGAAGTCTTG GGAAGATAAG
    CAGAAATGTG ATTCGGGTAA GCCAGTTCTC CGAACGCACT TGTACATCCA GCACGCGGCG
    GACCTGGCCA CGGAGGAAGT GTCTCAGATG CAGCTGTGCT CGCAGGCGGC CGAGGAGCTC
    ATCACCAGGA TCTGCGATGC GGCCACCATC CACTGTCTAT TGGAGCAGGA GCTGGCCCAC
    GCGGTGAACG CCTGCTCCCA CGCCCTGAAC AAAGCCAACC CACGCTTCCC AGAGAGTCTT
    ACAAGAGACA CTGCCACTGA AATAGCCATC AATGTGAAGG CCCTATATAA CGAAACAGAA
    TCTTTACTAG TGGGCAGGGT TCCTTTGCAA TTGGAATCTC CACATGAAGA GCGAGTATCC
    AATGCCTTAC ATTCCGTGGA AGTGGAGCTA CAGAAGTTAA CAGAGATTCC ATGGCTTTAT
    TATATCTTAC ACCCGAATGA GGATGAGGAG CCCCCCATGG ATTGCACCAA GAGAAATAGC
    AGAAGCACGG TATTTCGTAT TGTGCCCAAA TTCAAAAAGG AGAAGGTTCA GAAGCAGAAG
    ACAAGTTCAC AGCCTGGACC TGGGGATACC GAAAGTGGGT CATGTGAAGC GAATTCTCCA
    GGGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855792.1
    CDS36..3584
    Translation

    Target ORF information:

    RefSeq Version XM_025000024.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000024.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGCCGC GGCACGTGCG CCCCTCGCCG GGCGCCCGCA GCCCGACCTG GGAGGAACCG 
    GAGTCGGACA GGATGGCCGG GGCCGGCAGC CAACACCACC ACCCGGGCGC GGCGGGAGGA
    GCGGCAGGCG CTGCGGTCAC CCCCGCCACT GTCCCGGCGG GGCCCGGAGA CGACTCGTCA
    GACAGCGAAG CGGAGCAGGA GGGACCCCAG AAGCTGATCC GCAAAGTGTC CACCTCGGGG
    CAGATCCGCA CCAAGACCAG TATTAAAGAG GGACAGCTGC TGAAGCAAAC CAGTTCCTTC
    CAAAGGTGGA AAAAGCGATA CTTCAAACTT CGAGGTCGTA CACTTTATTA TGCAAAGGAC
    TCAAAGTCTC TGATATTTGA TGAAGTTGAC CTCTCAGATG CCAGTGTAGC TGAAGCAAGC
    ACGAAAAACG CTAACAACAG CTTCACGATA ATCACTCCAT TTAGAAGACT AATGCTGTGC
    GCTGAGAACA GGAAAGAGAT GGAGGATTGG ATCAGCTCGC TGAAGTCTGT ACAGACCAGA
    GAACCTTACG AGGTGGCCCA GTTTAACGTG GAACATTTCT CAGGGATGCA TAACTGGTAC
    GCGTGCTCCC ACGCCCGCCC CACTTTCTGT AACGTGTGCC GAGAGAGTCT TTCTGGAGTC
    ACCTCCCACG GCCTGTCCTG CGAAGTGTGT AAATTCAAGG CTCACAAACG CTGTGCAGTG
    AGGGCAATGA ACAACTGCAA GTGGACGACC CTGGCCTCCA TCGGGAAGGA CATCATCGAG
    AACGAGGACG GGGTGGCTAT GCCTCACCAG TGGCTCGAGG GTAACTTGCC CGTGAGCGCC
    AAGTGTGCCG TCTGCGACAA AACGTGCGGC AGTGTTCTCC GTCTTCAAGA TTGGAGATGC
    CTTTGGTGTA GAACGATGGT ACACACTGCC TGTAAAGATT TATATCATCC TGTATGTCCA
    CTTGGCCAAT GTAAAGTATC TGTCATACCT CCAATTGCAC TCAACAGCAC TGATTCTGAT
    GGTTTCTGTA GAGCAACATT TTCATTCTGT GTTAGTCCTT TATTGGTTTT TGTCAATTCT
    AAGAGTGGAG ATAATCAGGG AGTAAAGTTT CTTCGTCGAT TTAAACAGTT ACTAAATCCA
    GCTCAGGTGT TTGATTTGAT GAATGGCGGT CCTCATTTGG GTTTAAGATT ATTCCAGAAG
    TTTGACAATT TCCGGATTCT TGTTTGTGGA GGAGATGGAA GTGTAGGTTG GGTTTTGTCA
    GAAATTGATA AGCTCAACTT GAACAAACAG TGTCAGCTGG GAGTACTGCC TCTGGGGACA
    GGAAACGACC TTGCCCGGGT TCTCGGCTGG GGAGGCTCGT ACGATGACGA CACCCAACTT
    CCTCAGATAC TTGAGAAGCT GGAACGAGCC AGTACCAAAA TGTTGGACAG GTGGAGTATT
    ATGACCTATG AACTCAAATT GCCGCCAAAA GCTTCTCTAC TTCCCGAACC TCCAGAGGCG
    TCTGAAGAAT TTTATATGAC AATTTATGAA GACTCAGTTG CAGCTCATCT TACAAAAATC
    CTCAATTCTG ATGAACATGC AGTGGTCATA TCTTCTACCA AGATACTCTG TGAGACTGTA
    AAGGACTTCG TGGCCAAAGT AGAAAAAGCA CACGAGAAAA CGCTGGAAAA AGCTGTTGCA
    GCTGATGCTG TGGCCAATAA ATGTTCCATC CTAAATGAGA AGCTTGAACA ACTGCTCCAG
    GCTTTGCACA TAGAGTCCCA GGCAGGTCCA GTTCTCCCAG GCGTCAGCCC TCTCAGGGTA
    GAAGATGCTG TGGAATCTTC CAGCGAAGAA TCCTTGTGTG AGAGCAAGGA ACATCTCTTG
    GATGACACTA CGAAACCCTC TTCCCAGAAA GCTGTCAAAC CAAGGGAAAT AATGCTGCGG
    GCAAACAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG AGGCCAGAAA AGTGATGGAT
    GACCAGCCAG TTCACTCCTC TGAACTAGTT AATCAGTCAT TCGATTATGA TAGCACAGAA
    ACAGATGATT CCAAAGATGA GTTAAAAGAA GATGATGCGA AAGAGTCATT AACTGTTAAA
    GGTGCACCTC GGTCTCCGGA TGGCCGGGCT GGTCGTTCCC AAACTGACTC TGGCCCTGGT
    GCCCCTGTGG CAGCCAGCAA GGAAAACCTC CCTGTGCTCA ATACCAGAAT AATTTGCCCA
    GGTTTAAGAG CAGGACTAGC TGCCTCAATT GCTGGAAGTT CTATTATCAA CAAAATGTTG
    CTAGCAAATA TTGACCCTTT TGGTGCCACG CCATTTATTG ACCCAGATCC AGATTCAGTA
    GATGGCTATT CAGAAAAATG TGTCATGAAC AATTACTTTG GGATTGGATT AGATGCAAAA
    ATTTCATTAG AATTTAATAA TAAGAGAGAG GAGCACCCTG AAAAATGCAG GAGCCGAACT
    AAAAACTTGA TGTGGTATGG AGTCCTTGGA ACTCGGGAAT TATTACAAAG ATCATACAAG
    AATTTGGAAC AGAGGGTTCA ACTCGAATGT GACGGGCAGT ATATTCCTCT CCCCAGTCTG
    CAAGGAATAG CAGTGCTGAA CATCCCCAGC TATGCCGGAG GCACTAACTT TTGGGGTGGA
    ACTAAAGAAG ATGATATATT TGCTGCACCA TCATTTGATG ACAAGATCCT AGAAGTCGTT
    GCAATATTTG ACAGCGTGCA AATGGCCGTT TCAAGGGTCA TTAAACTGCA GCACCATCGA
    ATCGCCCAGT GCCGTACAGT AAAGATCACT ATCTTTGGTG ACGAGGGAGT CCCAGTGCAG
    GTGGACGGAG AAGCATGGGT TCAGCCTCCA GGGATTATCA AGATCGTGCA CAAGAACAGA
    GCTCAGATGC TCACCAGGGA CAGGGCCTTT GAAAGCACCC TGAAGTCTTG GGAAGATAAG
    CAGAAATGTG ATTCGGGTAA GCCAGTTCTC CGAACGCACT TGTACATCCA GCACGCGGCG
    GACCTGGCCA CGGAGGAAGT GTCTCAGATG CAGCTGTGCT CGCAGGCGGC CGAGGAGCTC
    ATCACCAGGA TCTGCGATGC GGCCACCATC CACTGTCTAT TGGAGCAGGA GCTGGCCCAC
    GCGGTGAACG CCTGCTCCCA CGCCCTGAAC AAAGCCAACC CACGCTTCCC AGAGAGTCTT
    ACAAGAGACA CTGCCACTGA AATAGCCATC AATGTGAAGG CCCTATATAA CGAAACAGAA
    TCTTTACTAG TGGGCAGGGT TCCTTTGCAA TTGGAATCTC CACATGAAGA GCGAGTATCC
    AATGCCTTAC ATTCCGTGGA AGTGGAGCTA CAGAAGTTAA CAGAGATTCC ATGGCTTTAT
    TATATCTTAC ACCCGAATGA GGATGAGGAG CCCCCCATGG ATTGCACCAA GAGAAATAGC
    AGAAGCACGG TATTTCGTAT TGTGCCCAAA TTCAAAAAGG AGAAGGTTCA GAAGCAGAAG
    ACAAGTTCAC AGCCTGGACC TGGGGATACC GAAAGTGGGT CATGTGAAGC GAATTCTCCA
    GGGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07196
    Clone ID Related Accession (Same CDS sequence) XM_015473789.1 , XM_010810743.2 , XM_015465679.1 , XM_015473789.2 , XM_002691824.4 , XM_617702.7 , XM_015465676.1
    Accession Version XM_015473789.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGTTCAG AAATGGGGCA CAGAGGAAGT TGCTGCTTGG
    CTGGATCTGC TCAATTTGGG AGAGTACAAA GAAATCTTCA TCCGCCATGA CATCAGAGGG
    GCTGAACTTT TGCATCTGGA AAGGCGAGAT CTTAAGGACC TGGGGATACC GAAAGTGGGT
    CATGTGAAGC GAATTCTCCA GGGAATTAAA GAGCTTGAAA GGAGCACCCT CCAGCCTGAG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329275.1
    CDS208..3453
    Translation

    Target ORF information:

    RefSeq Version XM_015473789.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473789.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGTTCAG AAATGGGGCA CAGAGGAAGT TGCTGCTTGG
    CTGGATCTGC TCAATTTGGG AGAGTACAAA GAAATCTTCA TCCGCCATGA CATCAGAGGG
    GCTGAACTTT TGCATCTGGA AAGGCGAGAT CTTAAGGACC TGGGGATACC GAAAGTGGGT
    CATGTGAAGC GAATTCTCCA GGGAATTAAA GAGCTTGAAA GGAGCACCCT CCAGCCTGAG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07196
    Clone ID Related Accession (Same CDS sequence) XM_015473789.1 , XM_010810743.2 , XM_015465679.1 , XM_015473789.2 , XM_002691824.4 , XM_617702.7 , XM_015465676.1
    Accession Version XM_010810743.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810743.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGTTCAG AAATGGGGCA CAGAGGAAGT TGCTGCTTGG
    CTGGATCTGC TCAATTTGGG AGAGTACAAA GAAATCTTCA TCCGCCATGA CATCAGAGGG
    GCTGAACTTT TGCATCTGGA AAGGCGAGAT CTTAAGGACC TGGGGATACC GAAAGTGGGT
    CATGTGAAGC GAATTCTCCA GGGAATTAAA GAGCTTGAAA GGAGCACCCT CCAGCCTGAG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010809045.1
    CDS1606..4851
    Translation

    Target ORF information:

    RefSeq Version XM_010810743.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810743.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGTTCAG AAATGGGGCA CAGAGGAAGT TGCTGCTTGG
    CTGGATCTGC TCAATTTGGG AGAGTACAAA GAAATCTTCA TCCGCCATGA CATCAGAGGG
    GCTGAACTTT TGCATCTGGA AAGGCGAGAT CTTAAGGACC TGGGGATACC GAAAGTGGGT
    CATGTGAAGC GAATTCTCCA GGGAATTAAA GAGCTTGAAA GGAGCACCCT CCAGCCTGAG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07196
    Clone ID Related Accession (Same CDS sequence) XM_015473789.1 , XM_010810743.2 , XM_015465679.1 , XM_015473789.2 , XM_002691824.4 , XM_617702.7 , XM_015465676.1
    Accession Version XM_015465679.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645949.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGTTCAG AAATGGGGCA CAGAGGAAGT TGCTGCTTGG
    CTGGATCTGC TCAATTTGGG AGAGTACAAA GAAATCTTCA TCCGCCATGA CATCAGAGGG
    GCTGAACTTT TGCATCTGGA AAGGCGAGAT CTTAAGGACC TGGGGATACC GAAAGTGGGT
    CATGTGAAGC GAATTCTCCA GGGAATTAAA GAGCTTGAAA GGAGCACCCT CCAGCCTGAG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321165.1
    CDS216..3461
    Translation

    Target ORF information:

    RefSeq Version XM_015465679.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465679.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGTTCAG AAATGGGGCA CAGAGGAAGT TGCTGCTTGG
    CTGGATCTGC TCAATTTGGG AGAGTACAAA GAAATCTTCA TCCGCCATGA CATCAGAGGG
    GCTGAACTTT TGCATCTGGA AAGGCGAGAT CTTAAGGACC TGGGGATACC GAAAGTGGGT
    CATGTGAAGC GAATTCTCCA GGGAATTAAA GAGCTTGAAA GGAGCACCCT CCAGCCTGAG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07196
    Clone ID Related Accession (Same CDS sequence) XM_015473789.1 , XM_010810743.2 , XM_015465679.1 , XM_015473789.2 , XM_002691824.4 , XM_617702.7 , XM_015465676.1
    Accession Version XM_015473789.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473789.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGTTCAG AAATGGGGCA CAGAGGAAGT TGCTGCTTGG
    CTGGATCTGC TCAATTTGGG AGAGTACAAA GAAATCTTCA TCCGCCATGA CATCAGAGGG
    GCTGAACTTT TGCATCTGGA AAGGCGAGAT CTTAAGGACC TGGGGATACC GAAAGTGGGT
    CATGTGAAGC GAATTCTCCA GGGAATTAAA GAGCTTGAAA GGAGCACCCT CCAGCCTGAG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329275.1
    CDS189..3434
    Translation

    Target ORF information:

    RefSeq Version XM_015473789.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473789.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGTTCAG AAATGGGGCA CAGAGGAAGT TGCTGCTTGG
    CTGGATCTGC TCAATTTGGG AGAGTACAAA GAAATCTTCA TCCGCCATGA CATCAGAGGG
    GCTGAACTTT TGCATCTGGA AAGGCGAGAT CTTAAGGACC TGGGGATACC GAAAGTGGGT
    CATGTGAAGC GAATTCTCCA GGGAATTAAA GAGCTTGAAA GGAGCACCCT CCAGCCTGAG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07196
    Clone ID Related Accession (Same CDS sequence) XM_015473789.1 , XM_010810743.2 , XM_015465679.1 , XM_015473789.2 , XM_002691824.4 , XM_617702.7 , XM_015465676.1
    Accession Version XM_002691824.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691824.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGTTCAG AAATGGGGCA CAGAGGAAGT TGCTGCTTGG
    CTGGATCTGC TCAATTTGGG AGAGTACAAA GAAATCTTCA TCCGCCATGA CATCAGAGGG
    GCTGAACTTT TGCATCTGGA AAGGCGAGAT CTTAAGGACC TGGGGATACC GAAAGTGGGT
    CATGTGAAGC GAATTCTCCA GGGAATTAAA GAGCTTGAAA GGAGCACCCT CCAGCCTGAG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002691870.2
    CDS216..3461
    Translation

    Target ORF information:

    RefSeq Version XM_002691824.4
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691824.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGTTCAG AAATGGGGCA CAGAGGAAGT TGCTGCTTGG
    CTGGATCTGC TCAATTTGGG AGAGTACAAA GAAATCTTCA TCCGCCATGA CATCAGAGGG
    GCTGAACTTT TGCATCTGGA AAGGCGAGAT CTTAAGGACC TGGGGATACC GAAAGTGGGT
    CATGTGAAGC GAATTCTCCA GGGAATTAAA GAGCTTGAAA GGAGCACCCT CCAGCCTGAG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07196
    Clone ID Related Accession (Same CDS sequence) XM_015473789.1 , XM_010810743.2 , XM_015465679.1 , XM_015473789.2 , XM_002691824.4 , XM_617702.7 , XM_015465676.1
    Accession Version XM_617702.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645949.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_617702.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGTTCAG AAATGGGGCA CAGAGGAAGT TGCTGCTTGG
    CTGGATCTGC TCAATTTGGG AGAGTACAAA GAAATCTTCA TCCGCCATGA CATCAGAGGG
    GCTGAACTTT TGCATCTGGA AAGGCGAGAT CTTAAGGACC TGGGGATACC GAAAGTGGGT
    CATGTGAAGC GAATTCTCCA GGGAATTAAA GAGCTTGAAA GGAGCACCCT CCAGCCTGAG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_617702.4
    CDS1606..4851
    Translation

    Target ORF information:

    RefSeq Version XM_617702.7
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_617702.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGTTCAG AAATGGGGCA CAGAGGAAGT TGCTGCTTGG
    CTGGATCTGC TCAATTTGGG AGAGTACAAA GAAATCTTCA TCCGCCATGA CATCAGAGGG
    GCTGAACTTT TGCATCTGGA AAGGCGAGAT CTTAAGGACC TGGGGATACC GAAAGTGGGT
    CATGTGAAGC GAATTCTCCA GGGAATTAAA GAGCTTGAAA GGAGCACCCT CCAGCCTGAG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07196
    Clone ID Related Accession (Same CDS sequence) XM_015473789.1 , XM_010810743.2 , XM_015465679.1 , XM_015473789.2 , XM_002691824.4 , XM_617702.7 , XM_015465676.1
    Accession Version XM_015465676.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645949.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGTTCAG AAATGGGGCA CAGAGGAAGT TGCTGCTTGG
    CTGGATCTGC TCAATTTGGG AGAGTACAAA GAAATCTTCA TCCGCCATGA CATCAGAGGG
    GCTGAACTTT TGCATCTGGA AAGGCGAGAT CTTAAGGACC TGGGGATACC GAAAGTGGGT
    CATGTGAAGC GAATTCTCCA GGGAATTAAA GAGCTTGAAA GGAGCACCCT CCAGCCTGAG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321162.1
    CDS208..3453
    Translation

    Target ORF information:

    RefSeq Version XM_015465676.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465676.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGTTCAG AAATGGGGCA CAGAGGAAGT TGCTGCTTGG
    CTGGATCTGC TCAATTTGGG AGAGTACAAA GAAATCTTCA TCCGCCATGA CATCAGAGGG
    GCTGAACTTT TGCATCTGGA AAGGCGAGAT CTTAAGGACC TGGGGATACC GAAAGTGGGT
    CATGTGAAGC GAATTCTCCA GGGAATTAAA GAGCTTGAAA GGAGCACCCT CCAGCCTGAG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07197
    Clone ID Related Accession (Same CDS sequence) XM_005213691.3 , XM_015465681.1
    Accession Version XM_005213691.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213691.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGGACCT GGGGATACCG AAAGTGGGTC ATGTGAAGCG
    AATTCTCCAG GGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213748.1
    CDS216..3293
    Translation

    Target ORF information:

    RefSeq Version XM_005213691.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213691.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGGACCT GGGGATACCG AAAGTGGGTC ATGTGAAGCG
    AATTCTCCAG GGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07197
    Clone ID Related Accession (Same CDS sequence) XM_005213691.3 , XM_015465681.1
    Accession Version XM_015465681.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645949.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGGACCT GGGGATACCG AAAGTGGGTC ATGTGAAGCG
    AATTCTCCAG GGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321167.1
    CDS216..3293
    Translation

    Target ORF information:

    RefSeq Version XM_015465681.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465681.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGTTAAAG GTGCACCTCG GTCTCCGGAT GGCCGGGCTG GTCGTTCCCA AACTGACTCT
    GGCCCTGGTG CCCCTGTGGC AGCCAGCAAG GAAAACCTCC CTGTGCTCAA TACCAGAATA
    ATTTGCCCAG GTTTAAGAGC AGGACTAGCT GCCTCAATTG CTGGAAGTTC TATTATCAAC
    AAAATGTTGC TAGCAAATAT TGACCCTTTT GGTGCCACGC CATTTATTGA CCCAGATCCA
    GATTCAGTAG ATGGCTATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA
    GATGCAAAAA TTTCATTAGA ATTTAATAAT AAGAGAGAGG AGCACCCTGA AAAATGCAGG
    AGCCGAACTA AAAACTTGAT GTGGTATGGA GTCCTTGGAA CTCGGGAATT ATTACAAAGA
    TCATACAAGA ATTTGGAACA GAGGGTTCAA CTCGAATGTG ACGGGCAGTA TATTCCTCTC
    CCCAGTCTGC AAGGAATAGC AGTGCTGAAC ATCCCCAGCT ATGCCGGAGG CACTAACTTT
    TGGGGTGGAA CTAAAGAAGA TGATATATTT GCTGCACCAT CATTTGATGA CAAGATCCTA
    GAAGTCGTTG CAATATTTGA CAGCGTGCAA ATGGCCGTTT CAAGGGTCAT TAAACTGCAG
    CACCATCGAA TCGCCCAGTG CCGTACAGTA AAGATCACTA TCTTTGGTGA CGAGGGAGTC
    CCAGTGCAGG TGGACGGAGA AGCATGGGTT CAGCCTCCAG GGATTATCAA GATCGTGCAC
    AAGAACAGAG CTCAGATGCT CACCAGGGAC AGGGCCTTTG AAAGCACCCT GAAGTCTTGG
    GAAGATAAGC AGAAATGTGA TTCGGGTAAG CCAGTTCTCC GAACGCACTT GTACATCCAG
    CACGCGGCGG ACCTGGCCAC GGAGGAAGTG TCTCAGATGC AGCTGTGCTC GCAGGCGGCC
    GAGGAGCTCA TCACCAGGAT CTGCGATGCG GCCACCATCC ACTGTCTATT GGAGCAGGAG
    CTGGCCCACG CGGTGAACGC CTGCTCCCAC GCCCTGAACA AAGCCAACCC ACGCTTCCCA
    GAGAGTCTTA CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC CCTATATAAC
    GAAACAGAAT CTTTACTAGT GGGCAGGGTT CCTTTGCAAT TGGAATCTCC ACATGAAGAG
    CGAGTATCCA ATGCCTTACA TTCCGTGGAA GTGGAGCTAC AGAAGTTAAC AGAGATTCCA
    TGGCTTTATT ATATCTTACA CCCGAATGAG GATGAGGAGC CCCCCATGGA TTGCACCAAG
    AGAAATAGCA GAAGCACGGT ATTTCGTATT GTGCCCAAAT TCAAAAAGGA GAAGGTTCAG
    AAGCAGAAGA CAAGTTCACA GCCTGGACCT GGGGATACCG AAAGTGGGTC ATGTGAAGCG
    AATTCTCCAG GGAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53690
    Clone ID Related Accession (Same CDS sequence) XM_015465678.1 , XM_010810744.2
    Accession Version XM_015465678.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3120bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645949.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGGTTTAA GAGCAGGACT AGCTGCCTCA ATTGCTGGAA GTTCTATTAT CAACAAAATG
    TTGCTAGCAA ATATTGACCC TTTTGGTGCC ACGCCATTTA TTGACCCAGA TCCAGATTCA
    GTAGATGGCT ATTCAGAAAA ATGTGTCATG AACAATTACT TTGGGATTGG ATTAGATGCA
    AAAATTTCAT TAGAATTTAA TAATAAGAGA GAGGAGCACC CTGAAAAATG CAGGAGCCGA
    ACTAAAAACT TGATGTGGTA TGGAGTCCTT GGAACTCGGG AATTATTACA AAGATCATAC
    AAGAATTTGG AACAGAGGGT TCAACTCGAA TGTGACGGGC AGTATATTCC TCTCCCCAGT
    CTGCAAGGAA TAGCAGTGCT GAACATCCCC AGCTATGCCG GAGGCACTAA CTTTTGGGGT
    GGAACTAAAG AAGATGATAT ATTTGCTGCA CCATCATTTG ATGACAAGAT CCTAGAAGTC
    GTTGCAATAT TTGACAGCGT GCAAATGGCC GTTTCAAGGG TCATTAAACT GCAGCACCAT
    CGAATCGCCC AGTGCCGTAC AGTAAAGATC ACTATCTTTG GTGACGAGGG AGTCCCAGTG
    CAGGTGGACG GAGAAGCATG GGTTCAGCCT CCAGGGATTA TCAAGATCGT GCACAAGAAC
    AGAGCTCAGA TGCTCACCAG GGACAGGGCC TTTGAAAGCA CCCTGAAGTC TTGGGAAGAT
    AAGCAGAAAT GTGATTCGGG TAAGCCAGTT CTCCGAACGC ACTTGTACAT CCAGCACGCG
    GCGGACCTGG CCACGGAGGA AGTGTCTCAG ATGCAGCTGT GCTCGCAGGC GGCCGAGGAG
    CTCATCACCA GGATCTGCGA TGCGGCCACC ATCCACTGTC TATTGGAGCA GGAGCTGGCC
    CACGCGGTGA ACGCCTGCTC CCACGCCCTG AACAAAGCCA ACCCACGCTT CCCAGAGAGT
    CTTACAAGAG ACACTGCCAC TGAAATAGCC ATCAATGTGA AGGCCCTATA TAACGAAACA
    GAATCTTTAC TAGTGGGCAG GGTTCCTTTG CAATTGGAAT CTCCACATGA AGAGCGAGTA
    TCCAATGCCT TACATTCCGT GGAAGTGGAG CTACAGAAGT TAACAGAGAT TCCATGGCTT
    TATTATATCT TACACCCGAA TGAGGATGAG GAGCCCCCCA TGGATTGCAC CAAGAGAAAT
    AGCAGAAGCA CGGTATTTCG TATTGTGCCC AAATTCAAAA AGGAGAAGGT TCAGAAGCAG
    AAGACAAGTT CACAGCCTGT TCAGAAATGG GGCACAGAGG AAGTTGCTGC TTGGCTGGAT
    CTGCTCAATT TGGGAGAGTA CAAAGAAATC TTCATCCGCC ATGACATCAG AGGGGCTGAA
    CTTTTGCATC TGGAAAGGCG AGATCTTAAG GACCTGGGGA TACCGAAAGT GGGTCATGTG
    AAGCGAATTC TCCAGGGAAT TAAAGAGCTT GAAAGGAGCA CCCTCCAGCC TGAGGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321164.1
    CDS100..3219
    Translation

    Target ORF information:

    RefSeq Version XM_015465678.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465678.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGGTTTAA GAGCAGGACT AGCTGCCTCA ATTGCTGGAA GTTCTATTAT CAACAAAATG
    TTGCTAGCAA ATATTGACCC TTTTGGTGCC ACGCCATTTA TTGACCCAGA TCCAGATTCA
    GTAGATGGCT ATTCAGAAAA ATGTGTCATG AACAATTACT TTGGGATTGG ATTAGATGCA
    AAAATTTCAT TAGAATTTAA TAATAAGAGA GAGGAGCACC CTGAAAAATG CAGGAGCCGA
    ACTAAAAACT TGATGTGGTA TGGAGTCCTT GGAACTCGGG AATTATTACA AAGATCATAC
    AAGAATTTGG AACAGAGGGT TCAACTCGAA TGTGACGGGC AGTATATTCC TCTCCCCAGT
    CTGCAAGGAA TAGCAGTGCT GAACATCCCC AGCTATGCCG GAGGCACTAA CTTTTGGGGT
    GGAACTAAAG AAGATGATAT ATTTGCTGCA CCATCATTTG ATGACAAGAT CCTAGAAGTC
    GTTGCAATAT TTGACAGCGT GCAAATGGCC GTTTCAAGGG TCATTAAACT GCAGCACCAT
    CGAATCGCCC AGTGCCGTAC AGTAAAGATC ACTATCTTTG GTGACGAGGG AGTCCCAGTG
    CAGGTGGACG GAGAAGCATG GGTTCAGCCT CCAGGGATTA TCAAGATCGT GCACAAGAAC
    AGAGCTCAGA TGCTCACCAG GGACAGGGCC TTTGAAAGCA CCCTGAAGTC TTGGGAAGAT
    AAGCAGAAAT GTGATTCGGG TAAGCCAGTT CTCCGAACGC ACTTGTACAT CCAGCACGCG
    GCGGACCTGG CCACGGAGGA AGTGTCTCAG ATGCAGCTGT GCTCGCAGGC GGCCGAGGAG
    CTCATCACCA GGATCTGCGA TGCGGCCACC ATCCACTGTC TATTGGAGCA GGAGCTGGCC
    CACGCGGTGA ACGCCTGCTC CCACGCCCTG AACAAAGCCA ACCCACGCTT CCCAGAGAGT
    CTTACAAGAG ACACTGCCAC TGAAATAGCC ATCAATGTGA AGGCCCTATA TAACGAAACA
    GAATCTTTAC TAGTGGGCAG GGTTCCTTTG CAATTGGAAT CTCCACATGA AGAGCGAGTA
    TCCAATGCCT TACATTCCGT GGAAGTGGAG CTACAGAAGT TAACAGAGAT TCCATGGCTT
    TATTATATCT TACACCCGAA TGAGGATGAG GAGCCCCCCA TGGATTGCAC CAAGAGAAAT
    AGCAGAAGCA CGGTATTTCG TATTGTGCCC AAATTCAAAA AGGAGAAGGT TCAGAAGCAG
    AAGACAAGTT CACAGCCTGT TCAGAAATGG GGCACAGAGG AAGTTGCTGC TTGGCTGGAT
    CTGCTCAATT TGGGAGAGTA CAAAGAAATC TTCATCCGCC ATGACATCAG AGGGGCTGAA
    CTTTTGCATC TGGAAAGGCG AGATCTTAAG GACCTGGGGA TACCGAAAGT GGGTCATGTG
    AAGCGAATTC TCCAGGGAAT TAAAGAGCTT GAAAGGAGCA CCCTCCAGCC TGAGGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53690
    Clone ID Related Accession (Same CDS sequence) XM_015465678.1 , XM_010810744.2
    Accession Version XM_010810744.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3120bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase eta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810744.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGGTTTAA GAGCAGGACT AGCTGCCTCA ATTGCTGGAA GTTCTATTAT CAACAAAATG
    TTGCTAGCAA ATATTGACCC TTTTGGTGCC ACGCCATTTA TTGACCCAGA TCCAGATTCA
    GTAGATGGCT ATTCAGAAAA ATGTGTCATG AACAATTACT TTGGGATTGG ATTAGATGCA
    AAAATTTCAT TAGAATTTAA TAATAAGAGA GAGGAGCACC CTGAAAAATG CAGGAGCCGA
    ACTAAAAACT TGATGTGGTA TGGAGTCCTT GGAACTCGGG AATTATTACA AAGATCATAC
    AAGAATTTGG AACAGAGGGT TCAACTCGAA TGTGACGGGC AGTATATTCC TCTCCCCAGT
    CTGCAAGGAA TAGCAGTGCT GAACATCCCC AGCTATGCCG GAGGCACTAA CTTTTGGGGT
    GGAACTAAAG AAGATGATAT ATTTGCTGCA CCATCATTTG ATGACAAGAT CCTAGAAGTC
    GTTGCAATAT TTGACAGCGT GCAAATGGCC GTTTCAAGGG TCATTAAACT GCAGCACCAT
    CGAATCGCCC AGTGCCGTAC AGTAAAGATC ACTATCTTTG GTGACGAGGG AGTCCCAGTG
    CAGGTGGACG GAGAAGCATG GGTTCAGCCT CCAGGGATTA TCAAGATCGT GCACAAGAAC
    AGAGCTCAGA TGCTCACCAG GGACAGGGCC TTTGAAAGCA CCCTGAAGTC TTGGGAAGAT
    AAGCAGAAAT GTGATTCGGG TAAGCCAGTT CTCCGAACGC ACTTGTACAT CCAGCACGCG
    GCGGACCTGG CCACGGAGGA AGTGTCTCAG ATGCAGCTGT GCTCGCAGGC GGCCGAGGAG
    CTCATCACCA GGATCTGCGA TGCGGCCACC ATCCACTGTC TATTGGAGCA GGAGCTGGCC
    CACGCGGTGA ACGCCTGCTC CCACGCCCTG AACAAAGCCA ACCCACGCTT CCCAGAGAGT
    CTTACAAGAG ACACTGCCAC TGAAATAGCC ATCAATGTGA AGGCCCTATA TAACGAAACA
    GAATCTTTAC TAGTGGGCAG GGTTCCTTTG CAATTGGAAT CTCCACATGA AGAGCGAGTA
    TCCAATGCCT TACATTCCGT GGAAGTGGAG CTACAGAAGT TAACAGAGAT TCCATGGCTT
    TATTATATCT TACACCCGAA TGAGGATGAG GAGCCCCCCA TGGATTGCAC CAAGAGAAAT
    AGCAGAAGCA CGGTATTTCG TATTGTGCCC AAATTCAAAA AGGAGAAGGT TCAGAAGCAG
    AAGACAAGTT CACAGCCTGT TCAGAAATGG GGCACAGAGG AAGTTGCTGC TTGGCTGGAT
    CTGCTCAATT TGGGAGAGTA CAAAGAAATC TTCATCCGCC ATGACATCAG AGGGGCTGAA
    CTTTTGCATC TGGAAAGGCG AGATCTTAAG GACCTGGGGA TACCGAAAGT GGGTCATGTG
    AAGCGAATTC TCCAGGGAAT TAAAGAGCTT GAAAGGAGCA CCCTCCAGCC TGAGGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010809046.1
    CDS100..3219
    Translation

    Target ORF information:

    RefSeq Version XM_010810744.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase eta (DGKH), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810744.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGTGCG CTGAGAACAG GAAAGAGATG GAGGATTGGA TCAGCTCGCT GAAGTCTGTA 
    CAGACCAGAG AACCTTACGA GGTGGCCCAG TTTAACGTGG AACATTTCTC AGGGATGCAT
    AACTGGTACG CGTGCTCCCA CGCCCGCCCC ACTTTCTGTA ACGTGTGCCG AGAGAGTCTT
    TCTGGAGTCA CCTCCCACGG CCTGTCCTGC GAAGTGTGTA AATTCAAGGC TCACAAACGC
    TGTGCAGTGA GGGCAATGAA CAACTGCAAG TGGACGACCC TGGCCTCCAT CGGGAAGGAC
    ATCATCGAGA ACGAGGACGG GGTGGCTATG CCTCACCAGT GGCTCGAGGG TAACTTGCCC
    GTGAGCGCCA AGTGTGCCGT CTGCGACAAA ACGTGCGGCA GTGTTCTCCG TCTTCAAGAT
    TGGAGATGCC TTTGGTGTAG AACGATGGTA CACACTGCCT GTAAAGATTT ATATCATCCT
    GTATGTCCAC TTGGCCAATG TAAAGTATCT GTCATACCTC CAATTGCACT CAACAGCACT
    GATTCTGATG GTTTCTGTAG AGCAACATTT TCATTCTGTG TTAGTCCTTT ATTGGTTTTT
    GTCAATTCTA AGAGTGGAGA TAATCAGGGA GTAAAGTTTC TTCGTCGATT TAAACAGTTA
    CTAAATCCAG CTCAGGTGTT TGATTTGATG AATGGCGGTC CTCATTTGGG TTTAAGATTA
    TTCCAGAAGT TTGACAATTT CCGGATTCTT GTTTGTGGAG GAGATGGAAG TGTAGGTTGG
    GTTTTGTCAG AAATTGATAA GCTCAACTTG AACAAACAGT GTCAGCTGGG AGTACTGCCT
    CTGGGGACAG GAAACGACCT TGCCCGGGTT CTCGGCTGGG GAGGCTCGTA CGATGACGAC
    ACCCAACTTC CTCAGATACT TGAGAAGCTG GAACGAGCCA GTACCAAAAT GTTGGACAGG
    TGGAGTATTA TGACCTATGA ACTCAAATTG CCGCCAAAAG CTTCTCTACT TCCCGAACCT
    CCAGAGGCGT CTGAAGAATT TTATATGACA ATTTATGAAG ACTCAGTTGC AGCTCATCTT
    ACAAAAATCC TCAATTCTGA TGAACATGCA GTGGTCATAT CTTCTACCAA GATACTCTGT
    GAGACTGTAA AGGACTTCGT GGCCAAAGTA GAAAAAGCAC ACGAGAAAAC GCTGGAAAAA
    GCTGTTGCAG CTGATGCTGT GGCCAATAAA TGTTCCATCC TAAATGAGAA GCTTGAACAA
    CTGCTCCAGG CTTTGCACAT AGAGTCCCAG GCAGGTCCAG TTCTCCCAGG CGTCAGCCCT
    CTCAGGGTAG AAGATGCTGT GGAATCTTCC AGCGAAGAAT CCTTGTGTGA GAGCAAGGAA
    CATCTCTTGG ATGACACTAC GAAACCCTCT TCCCAGAAAG CTGTCAAACC AAGGGAAATA
    ATGCTGCGGG CAAACAGTTT AAAGAAAGCA GTGAGGCAAG TCATTGAGGA GGCCAGAAAA
    GTGATGGATG ACCAGCCAGT TCACTCCTCT GAACTAGTTA ATCAGTCATT CGATTATGAT
    AGCACAGAAA CAGATGATTC CAAAGATGAG TTAAAAGAAG ATGATGCGAA AGAGTCATTA
    ACTGGTTTAA GAGCAGGACT AGCTGCCTCA ATTGCTGGAA GTTCTATTAT CAACAAAATG
    TTGCTAGCAA ATATTGACCC TTTTGGTGCC ACGCCATTTA TTGACCCAGA TCCAGATTCA
    GTAGATGGCT ATTCAGAAAA ATGTGTCATG AACAATTACT TTGGGATTGG ATTAGATGCA
    AAAATTTCAT TAGAATTTAA TAATAAGAGA GAGGAGCACC CTGAAAAATG CAGGAGCCGA
    ACTAAAAACT TGATGTGGTA TGGAGTCCTT GGAACTCGGG AATTATTACA AAGATCATAC
    AAGAATTTGG AACAGAGGGT TCAACTCGAA TGTGACGGGC AGTATATTCC TCTCCCCAGT
    CTGCAAGGAA TAGCAGTGCT GAACATCCCC AGCTATGCCG GAGGCACTAA CTTTTGGGGT
    GGAACTAAAG AAGATGATAT ATTTGCTGCA CCATCATTTG ATGACAAGAT CCTAGAAGTC
    GTTGCAATAT TTGACAGCGT GCAAATGGCC GTTTCAAGGG TCATTAAACT GCAGCACCAT
    CGAATCGCCC AGTGCCGTAC AGTAAAGATC ACTATCTTTG GTGACGAGGG AGTCCCAGTG
    CAGGTGGACG GAGAAGCATG GGTTCAGCCT CCAGGGATTA TCAAGATCGT GCACAAGAAC
    AGAGCTCAGA TGCTCACCAG GGACAGGGCC TTTGAAAGCA CCCTGAAGTC TTGGGAAGAT
    AAGCAGAAAT GTGATTCGGG TAAGCCAGTT CTCCGAACGC ACTTGTACAT CCAGCACGCG
    GCGGACCTGG CCACGGAGGA AGTGTCTCAG ATGCAGCTGT GCTCGCAGGC GGCCGAGGAG
    CTCATCACCA GGATCTGCGA TGCGGCCACC ATCCACTGTC TATTGGAGCA GGAGCTGGCC
    CACGCGGTGA ACGCCTGCTC CCACGCCCTG AACAAAGCCA ACCCACGCTT CCCAGAGAGT
    CTTACAAGAG ACACTGCCAC TGAAATAGCC ATCAATGTGA AGGCCCTATA TAACGAAACA
    GAATCTTTAC TAGTGGGCAG GGTTCCTTTG CAATTGGAAT CTCCACATGA AGAGCGAGTA
    TCCAATGCCT TACATTCCGT GGAAGTGGAG CTACAGAAGT TAACAGAGAT TCCATGGCTT
    TATTATATCT TACACCCGAA TGAGGATGAG GAGCCCCCCA TGGATTGCAC CAAGAGAAAT
    AGCAGAAGCA CGGTATTTCG TATTGTGCCC AAATTCAAAA AGGAGAAGGT TCAGAAGCAG
    AAGACAAGTT CACAGCCTGT TCAGAAATGG GGCACAGAGG AAGTTGCTGC TTGGCTGGAT
    CTGCTCAATT TGGGAGAGTA CAAAGAAATC TTCATCCGCC ATGACATCAG AGGGGCTGAA
    CTTTTGCATC TGGAAAGGCG AGATCTTAAG GACCTGGGGA TACCGAAAGT GGGTCATGTG
    AAGCGAATTC TCCAGGGAAT TAAAGAGCTT GAAAGGAGCA CCCTCCAGCC TGAGGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.