ANKLE1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKLE1
Entrez Gene ID	537502
Full Name	ankyrin repeat and LEM domain containing 1
Synonyms	ANKRD41
General protein information	Preferred Names ankyrin repeat and LEM domain containing 1 Names ankyrin repeat and LEM domain-containing protein 1 ankyrin repeat domain 41
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002688578.6	XP_002688624.2	ankyrin repeat and LEM domain-containing protein 1 isoform X2
XM_005198818.3	XP_005198875.1	ankyrin repeat and LEM domain-containing protein 1 isoform X1
XM_005208558.3	XP_005208615.1	ankyrin repeat and LEM domain-containing protein 1 isoform X1
XM_617670.9	XP_617670.4	ankyrin repeat and LEM domain-containing protein 1 isoform X2
XM_002688578.5	XP_002688624.2	ankyrin repeat and LEM domain-containing protein 1 isoform X2
XM_005208558.4	XP_005208615.1	ankyrin repeat and LEM domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ANKLE1	NP_689576.5
Bos taurus (cattle)	ANKLE1	XP_005208615.1
	ANKLE1	XP_001114324.2
Rattus norvegicus (Norway rat)	Ankle1	XP_006253022.1
Pan troglodytes (chimpanzee)	ANKLE1	XP_001173375.2
Canis lupus familiaris (dog)	ANKLE1	XP_852664.2
Mus musculus (house mouse)	Ankle1	NP_766344.2

Gene Ontology

The following ANKLE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKLE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa05041	XM_002688578.6 Latest version!	Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa05041	XM_617670.9 Latest version!	Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa05040	XM_005208558.4 Latest version!	Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa05040	XM_005198818.3 Latest version!	Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OBa05041	XM_002688578.5	Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa05040	XM_005208558.3	Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKLE1 ( XM_002688578.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002688624.2 Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa05041
Clone ID Related Accession (Same CDS sequence)	XM_002688578.6 , XM_617670.9 , XM_002688578.5
Accession Version	XM_002688578.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002688578.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCGA CAATGCGGCG CCGGTCAGCC TGGCTGGGGT CGCTGCACTG CACCAAGGCG 
CGGAGAAGGG AGCCGAGGGG CGGAGCGGCG CGTGCGCACA AGGGCGCCGC GGGAAGTTGG 
AAACCGGCAG GCGGCGTGAC CAGGTGGCTG CCCCAAGGGG ACAGCCGGGG CATGGGCCCA 
GCTGTAGGTT TGGCGCGGCG GCTGCGGACG GCGCTGCAGG AGGAGGAGTT GCGATCTGTG 
GAGGAGTTGC TGCGCCGCGG TGCCGACCCC AATCTGGTAC TCGAGGATGG CGCGGCGGCC 
ATGCACCTGG CGGCCAGAGC CCAGCACCTA CGGAGTCTGT GTTGCCTCGA GGCCCTGCTG 
CGCCGAGGCG GGGACCCCAA CGCTCGATCG GCCGAGGCAC TGACACCGCT GCATGTGGCC 
GCTGCCTGGG GCTGTCGCCG GGGCCTGGAA CTGCTGCTGA GCCAGGGAGC GGACCCCGCG 
CTCCGCGACC AGGACGGATT CCTGCCTCTG GACCTGGCGG AGCAGCAGGG GCACCAGAAC 
TGTGCCCGTC TGCTTCGGGA GCTGGAGGCT CGGACCAGGA CCCCAACCCG GACCTGGAAA 
GGCTCCCGGA AGCCAGAACC CGAGCCAGAG CCTGGAGGCC CAGGCCCTTG TGAAAAGAGG 
CTGGATACCA GCCCCTCCGG TCCTCCTCAT GTGATGCTGA ACTCTACAAC ACTGGGCACA 
GGTGACGGCA AGGACGTGAG CCTGGAGGCT GGCCCTGGAC CCCCCGGCCT GCTTGCCCAC 
CCTGAGACTG CTGACACAGA TGGTGGATTG GAGTCCCCCC TGGGGCGCTG GGACTGCAGC 
TCGGTGACCT CCTTTGTCAC CGCTGTTGAG GCCTCAGGAG CTGAGGACCT AACTGGCCAC 
ACCTCCCCCT GGGCTGGTGT CCGACTCTCC CAGAGGGTGC CAAGGTCTTT GGGTACCCTA 
CAGCTGGCGC ATCAACCCCT CATGGAGGAC AGGGAGGCAG AACTAAATGC CCATCTGCAG 
GCCCTGACTC TGACCTTGCC AGATGATTCC CCCTCCCCCA AGTTCTTCCC AGACAGGAGC 
CCAACCCATA GTCCCCCTAG GGAACTGCTG CCTGGACCTT CTGACACCCA CATCACAAGA 
GAGGACCAGC TATCCCCCGA CGGTGATTTG GCTGCCCTCT GGCTGACAGA GGATGAGGCG 
AGCGCCACAG TGGGCAGGGA CCCCAGCCCC TCTGATCAGT GTCCACCGGA CCCTACCATG 
TCTGACCTGG AGCTGCTGCA AGGGCTCCGG GCGCTTGGCA AGAGCCCGGG TCCCATCACA 
TCCTTCACCC GGCCACGCTA CCTCCGACGG CTGAAAGAAG CCCAGGCTGC TCCTAGCCCA 
GACTTTTCAG GGCACAGCCC AGAGCTGGCC GAAGCCCTGC GGACAGGCCG TATCCCAGAT 
GCCCAAGCCG ATGAAGATGC ACTTGCCCAG CAGTTCGAGC GGCCAGACCC CACCAGACGG 
TGGCGGGAGG GAGTCGTGAA GTCCAGCTTC ACATATCTGC TGCTGGACCC CAGGCCATCT 
TCTACGTGGG CAAGGGAACA CGGGCCCGGC CAGATGTTCA CCTCTGGGAG GCCCTTAGCC 
ACCGAAGACG GCCAGGAAAA CAGGCCTGTC CCAAGGTACG CCGGATCTTG GACATCTGGG 
CCACTGGTCG TGGCATTGTT TCCCTGCATT GCTTCCAGCA TGTGGTCCCT GCAGAGGCTT 
ACACTCGAGA AGCGTGTCTT GTGGATGCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002688624.2
CDS	954..2726
Translation	MWATMRRRSAWLGSLHCTKARRREPRGGAARAHKGAAGSWKPAGGVTRWLPQGDSRGMGPAVGLARRLRTALQEEELRSVEELLRRGADPNLVLEDGAAAMHLAARAQHLRSLCCLEALLRRGGDPNARSAEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGFLPLDLAEQQGHQNCARLLRELEARTRTPTRTWKGSRKPEPEPEPGGPGPCEKRLDTSPSGPPHVMLNSTTLGTGDGKDVSLEAGPGPPGLLAHPETADTDGGLESPLGRWDCSSVTSFVTAVEASGAEDLTGHTSPWAGVRLSQRVPRSLGTLQLAHQPLMEDREAELNAHLQALTLTLPDDSPSPKFFPDRSPTHSPPRELLPGPSDTHITREDQLSPDGDLAALWLTEDEASATVGRDPSPSDQCPPDPTMSDLELLQGLRALGKSPGPITSFTRPRYLRRLKEAQAAPSPDFSGHSPELAEALRTGRIPDAQADEDALAQQFERPDPTRRWREGVVKSSFTYLLLDPRPSSTWAREHGPGQMFTSGRPLATEDGQENRPVPRYAGSWTSGPLVVALFPCIASSMWSLQRLTLEKRVLWML

Target ORF information:

RefSeq Version	XM_002688578.6
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002688578.6

ORF Insert Sequence:

ATGTGGGCGA CAATGCGGCG CCGGTCAGCC TGGCTGGGGT CGCTGCACTG CACCAAGGCG 
CGGAGAAGGG AGCCGAGGGG CGGAGCGGCG CGTGCGCACA AGGGCGCCGC GGGAAGTTGG 
AAACCGGCAG GCGGCGTGAC CAGGTGGCTG CCCCAAGGGG ACAGCCGGGG CATGGGCCCA 
GCTGTAGGTT TGGCGCGGCG GCTGCGGACG GCGCTGCAGG AGGAGGAGTT GCGATCTGTG 
GAGGAGTTGC TGCGCCGCGG TGCCGACCCC AATCTGGTAC TCGAGGATGG CGCGGCGGCC 
ATGCACCTGG CGGCCAGAGC CCAGCACCTA CGGAGTCTGT GTTGCCTCGA GGCCCTGCTG 
CGCCGAGGCG GGGACCCCAA CGCTCGATCG GCCGAGGCAC TGACACCGCT GCATGTGGCC 
GCTGCCTGGG GCTGTCGCCG GGGCCTGGAA CTGCTGCTGA GCCAGGGAGC GGACCCCGCG 
CTCCGCGACC AGGACGGATT CCTGCCTCTG GACCTGGCGG AGCAGCAGGG GCACCAGAAC 
TGTGCCCGTC TGCTTCGGGA GCTGGAGGCT CGGACCAGGA CCCCAACCCG GACCTGGAAA 
GGCTCCCGGA AGCCAGAACC CGAGCCAGAG CCTGGAGGCC CAGGCCCTTG TGAAAAGAGG 
CTGGATACCA GCCCCTCCGG TCCTCCTCAT GTGATGCTGA ACTCTACAAC ACTGGGCACA 
GGTGACGGCA AGGACGTGAG CCTGGAGGCT GGCCCTGGAC CCCCCGGCCT GCTTGCCCAC 
CCTGAGACTG CTGACACAGA TGGTGGATTG GAGTCCCCCC TGGGGCGCTG GGACTGCAGC 
TCGGTGACCT CCTTTGTCAC CGCTGTTGAG GCCTCAGGAG CTGAGGACCT AACTGGCCAC 
ACCTCCCCCT GGGCTGGTGT CCGACTCTCC CAGAGGGTGC CAAGGTCTTT GGGTACCCTA 
CAGCTGGCGC ATCAACCCCT CATGGAGGAC AGGGAGGCAG AACTAAATGC CCATCTGCAG 
GCCCTGACTC TGACCTTGCC AGATGATTCC CCCTCCCCCA AGTTCTTCCC AGACAGGAGC 
CCAACCCATA GTCCCCCTAG GGAACTGCTG CCTGGACCTT CTGACACCCA CATCACAAGA 
GAGGACCAGC TATCCCCCGA CGGTGATTTG GCTGCCCTCT GGCTGACAGA GGATGAGGCG 
AGCGCCACAG TGGGCAGGGA CCCCAGCCCC TCTGATCAGT GTCCACCGGA CCCTACCATG 
TCTGACCTGG AGCTGCTGCA AGGGCTCCGG GCGCTTGGCA AGAGCCCGGG TCCCATCACA 
TCCTTCACCC GGCCACGCTA CCTCCGACGG CTGAAAGAAG CCCAGGCTGC TCCTAGCCCA 
GACTTTTCAG GGCACAGCCC AGAGCTGGCC GAAGCCCTGC GGACAGGCCG TATCCCAGAT 
GCCCAAGCCG ATGAAGATGC ACTTGCCCAG CAGTTCGAGC GGCCAGACCC CACCAGACGG 
TGGCGGGAGG GAGTCGTGAA GTCCAGCTTC ACATATCTGC TGCTGGACCC CAGGCCATCT 
TCTACGTGGG CAAGGGAACA CGGGCCCGGC CAGATGTTCA CCTCTGGGAG GCCCTTAGCC 
ACCGAAGACG GCCAGGAAAA CAGGCCTGTC CCAAGGTACG CCGGATCTTG GACATCTGGG 
CCACTGGTCG TGGCATTGTT TCCCTGCATT GCTTCCAGCA TGTGGTCCCT GCAGAGGCTT 
ACACTCGAGA AGCGTGTCTT GTGGATGCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE1 ( XM_617670.9 ) cDNA ORF clone, Bos taurus(cattle) -> XP_617670.4 Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa05041
Clone ID Related Accession (Same CDS sequence)	XM_002688578.6 , XM_617670.9 , XM_002688578.5
Accession Version	XM_617670.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645005.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_617670.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCGA CAATGCGGCG CCGGTCAGCC TGGCTGGGGT CGCTGCACTG CACCAAGGCG 
CGGAGAAGGG AGCCGAGGGG CGGAGCGGCG CGTGCGCACA AGGGCGCCGC GGGAAGTTGG 
AAACCGGCAG GCGGCGTGAC CAGGTGGCTG CCCCAAGGGG ACAGCCGGGG CATGGGCCCA 
GCTGTAGGTT TGGCGCGGCG GCTGCGGACG GCGCTGCAGG AGGAGGAGTT GCGATCTGTG 
GAGGAGTTGC TGCGCCGCGG TGCCGACCCC AATCTGGTAC TCGAGGATGG CGCGGCGGCC 
ATGCACCTGG CGGCCAGAGC CCAGCACCTA CGGAGTCTGT GTTGCCTCGA GGCCCTGCTG 
CGCCGAGGCG GGGACCCCAA CGCTCGATCG GCCGAGGCAC TGACACCGCT GCATGTGGCC 
GCTGCCTGGG GCTGTCGCCG GGGCCTGGAA CTGCTGCTGA GCCAGGGAGC GGACCCCGCG 
CTCCGCGACC AGGACGGATT CCTGCCTCTG GACCTGGCGG AGCAGCAGGG GCACCAGAAC 
TGTGCCCGTC TGCTTCGGGA GCTGGAGGCT CGGACCAGGA CCCCAACCCG GACCTGGAAA 
GGCTCCCGGA AGCCAGAACC CGAGCCAGAG CCTGGAGGCC CAGGCCCTTG TGAAAAGAGG 
CTGGATACCA GCCCCTCCGG TCCTCCTCAT GTGATGCTGA ACTCTACAAC ACTGGGCACA 
GGTGACGGCA AGGACGTGAG CCTGGAGGCT GGCCCTGGAC CCCCCGGCCT GCTTGCCCAC 
CCTGAGACTG CTGACACAGA TGGTGGATTG GAGTCCCCCC TGGGGCGCTG GGACTGCAGC 
TCGGTGACCT CCTTTGTCAC CGCTGTTGAG GCCTCAGGAG CTGAGGACCT AACTGGCCAC 
ACCTCCCCCT GGGCTGGTGT CCGACTCTCC CAGAGGGTGC CAAGGTCTTT GGGTACCCTA 
CAGCTGGCGC ATCAACCCCT CATGGAGGAC AGGGAGGCAG AACTAAATGC CCATCTGCAG 
GCCCTGACTC TGACCTTGCC AGATGATTCC CCCTCCCCCA AGTTCTTCCC AGACAGGAGC 
CCAACCCATA GTCCCCCTAG GGAACTGCTG CCTGGACCTT CTGACACCCA CATCACAAGA 
GAGGACCAGC TATCCCCCGA CGGTGATTTG GCTGCCCTCT GGCTGACAGA GGATGAGGCG 
AGCGCCACAG TGGGCAGGGA CCCCAGCCCC TCTGATCAGT GTCCACCGGA CCCTACCATG 
TCTGACCTGG AGCTGCTGCA AGGGCTCCGG GCGCTTGGCA AGAGCCCGGG TCCCATCACA 
TCCTTCACCC GGCCACGCTA CCTCCGACGG CTGAAAGAAG CCCAGGCTGC TCCTAGCCCA 
GACTTTTCAG GGCACAGCCC AGAGCTGGCC GAAGCCCTGC GGACAGGCCG TATCCCAGAT 
GCCCAAGCCG ATGAAGATGC ACTTGCCCAG CAGTTCGAGC GGCCAGACCC CACCAGACGG 
TGGCGGGAGG GAGTCGTGAA GTCCAGCTTC ACATATCTGC TGCTGGACCC CAGGCCATCT 
TCTACGTGGG CAAGGGAACA CGGGCCCGGC CAGATGTTCA CCTCTGGGAG GCCCTTAGCC 
ACCGAAGACG GCCAGGAAAA CAGGCCTGTC CCAAGGTACG CCGGATCTTG GACATCTGGG 
CCACTGGTCG TGGCATTGTT TCCCTGCATT GCTTCCAGCA TGTGGTCCCT GCAGAGGCTT 
ACACTCGAGA AGCGTGTCTT GTGGATGCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_617670.4
CDS	971..2743
Translation	MWATMRRRSAWLGSLHCTKARRREPRGGAARAHKGAAGSWKPAGGVTRWLPQGDSRGMGPAVGLARRLRTALQEEELRSVEELLRRGADPNLVLEDGAAAMHLAARAQHLRSLCCLEALLRRGGDPNARSAEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGFLPLDLAEQQGHQNCARLLRELEARTRTPTRTWKGSRKPEPEPEPGGPGPCEKRLDTSPSGPPHVMLNSTTLGTGDGKDVSLEAGPGPPGLLAHPETADTDGGLESPLGRWDCSSVTSFVTAVEASGAEDLTGHTSPWAGVRLSQRVPRSLGTLQLAHQPLMEDREAELNAHLQALTLTLPDDSPSPKFFPDRSPTHSPPRELLPGPSDTHITREDQLSPDGDLAALWLTEDEASATVGRDPSPSDQCPPDPTMSDLELLQGLRALGKSPGPITSFTRPRYLRRLKEAQAAPSPDFSGHSPELAEALRTGRIPDAQADEDALAQQFERPDPTRRWREGVVKSSFTYLLLDPRPSSTWAREHGPGQMFTSGRPLATEDGQENRPVPRYAGSWTSGPLVVALFPCIASSMWSLQRLTLEKRVLWML

Target ORF information:

RefSeq Version	XM_617670.9
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_617670.9

ORF Insert Sequence:

ATGTGGGCGA CAATGCGGCG CCGGTCAGCC TGGCTGGGGT CGCTGCACTG CACCAAGGCG 
CGGAGAAGGG AGCCGAGGGG CGGAGCGGCG CGTGCGCACA AGGGCGCCGC GGGAAGTTGG 
AAACCGGCAG GCGGCGTGAC CAGGTGGCTG CCCCAAGGGG ACAGCCGGGG CATGGGCCCA 
GCTGTAGGTT TGGCGCGGCG GCTGCGGACG GCGCTGCAGG AGGAGGAGTT GCGATCTGTG 
GAGGAGTTGC TGCGCCGCGG TGCCGACCCC AATCTGGTAC TCGAGGATGG CGCGGCGGCC 
ATGCACCTGG CGGCCAGAGC CCAGCACCTA CGGAGTCTGT GTTGCCTCGA GGCCCTGCTG 
CGCCGAGGCG GGGACCCCAA CGCTCGATCG GCCGAGGCAC TGACACCGCT GCATGTGGCC 
GCTGCCTGGG GCTGTCGCCG GGGCCTGGAA CTGCTGCTGA GCCAGGGAGC GGACCCCGCG 
CTCCGCGACC AGGACGGATT CCTGCCTCTG GACCTGGCGG AGCAGCAGGG GCACCAGAAC 
TGTGCCCGTC TGCTTCGGGA GCTGGAGGCT CGGACCAGGA CCCCAACCCG GACCTGGAAA 
GGCTCCCGGA AGCCAGAACC CGAGCCAGAG CCTGGAGGCC CAGGCCCTTG TGAAAAGAGG 
CTGGATACCA GCCCCTCCGG TCCTCCTCAT GTGATGCTGA ACTCTACAAC ACTGGGCACA 
GGTGACGGCA AGGACGTGAG CCTGGAGGCT GGCCCTGGAC CCCCCGGCCT GCTTGCCCAC 
CCTGAGACTG CTGACACAGA TGGTGGATTG GAGTCCCCCC TGGGGCGCTG GGACTGCAGC 
TCGGTGACCT CCTTTGTCAC CGCTGTTGAG GCCTCAGGAG CTGAGGACCT AACTGGCCAC 
ACCTCCCCCT GGGCTGGTGT CCGACTCTCC CAGAGGGTGC CAAGGTCTTT GGGTACCCTA 
CAGCTGGCGC ATCAACCCCT CATGGAGGAC AGGGAGGCAG AACTAAATGC CCATCTGCAG 
GCCCTGACTC TGACCTTGCC AGATGATTCC CCCTCCCCCA AGTTCTTCCC AGACAGGAGC 
CCAACCCATA GTCCCCCTAG GGAACTGCTG CCTGGACCTT CTGACACCCA CATCACAAGA 
GAGGACCAGC TATCCCCCGA CGGTGATTTG GCTGCCCTCT GGCTGACAGA GGATGAGGCG 
AGCGCCACAG TGGGCAGGGA CCCCAGCCCC TCTGATCAGT GTCCACCGGA CCCTACCATG 
TCTGACCTGG AGCTGCTGCA AGGGCTCCGG GCGCTTGGCA AGAGCCCGGG TCCCATCACA 
TCCTTCACCC GGCCACGCTA CCTCCGACGG CTGAAAGAAG CCCAGGCTGC TCCTAGCCCA 
GACTTTTCAG GGCACAGCCC AGAGCTGGCC GAAGCCCTGC GGACAGGCCG TATCCCAGAT 
GCCCAAGCCG ATGAAGATGC ACTTGCCCAG CAGTTCGAGC GGCCAGACCC CACCAGACGG 
TGGCGGGAGG GAGTCGTGAA GTCCAGCTTC ACATATCTGC TGCTGGACCC CAGGCCATCT 
TCTACGTGGG CAAGGGAACA CGGGCCCGGC CAGATGTTCA CCTCTGGGAG GCCCTTAGCC 
ACCGAAGACG GCCAGGAAAA CAGGCCTGTC CCAAGGTACG CCGGATCTTG GACATCTGGG 
CCACTGGTCG TGGCATTGTT TCCCTGCATT GCTTCCAGCA TGTGGTCCCT GCAGAGGCTT 
ACACTCGAGA AGCGTGTCTT GTGGATGCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE1 ( XM_002688578.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002688624.2 Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa05041
Clone ID Related Accession (Same CDS sequence)	XM_002688578.6 , XM_617670.9 , XM_002688578.5
Accession Version	XM_002688578.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002688578.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCGA CAATGCGGCG CCGGTCAGCC TGGCTGGGGT CGCTGCACTG CACCAAGGCG 
CGGAGAAGGG AGCCGAGGGG CGGAGCGGCG CGTGCGCACA AGGGCGCCGC GGGAAGTTGG 
AAACCGGCAG GCGGCGTGAC CAGGTGGCTG CCCCAAGGGG ACAGCCGGGG CATGGGCCCA 
GCTGTAGGTT TGGCGCGGCG GCTGCGGACG GCGCTGCAGG AGGAGGAGTT GCGATCTGTG 
GAGGAGTTGC TGCGCCGCGG TGCCGACCCC AATCTGGTAC TCGAGGATGG CGCGGCGGCC 
ATGCACCTGG CGGCCAGAGC CCAGCACCTA CGGAGTCTGT GTTGCCTCGA GGCCCTGCTG 
CGCCGAGGCG GGGACCCCAA CGCTCGATCG GCCGAGGCAC TGACACCGCT GCATGTGGCC 
GCTGCCTGGG GCTGTCGCCG GGGCCTGGAA CTGCTGCTGA GCCAGGGAGC GGACCCCGCG 
CTCCGCGACC AGGACGGATT CCTGCCTCTG GACCTGGCGG AGCAGCAGGG GCACCAGAAC 
TGTGCCCGTC TGCTTCGGGA GCTGGAGGCT CGGACCAGGA CCCCAACCCG GACCTGGAAA 
GGCTCCCGGA AGCCAGAACC CGAGCCAGAG CCTGGAGGCC CAGGCCCTTG TGAAAAGAGG 
CTGGATACCA GCCCCTCCGG TCCTCCTCAT GTGATGCTGA ACTCTACAAC ACTGGGCACA 
GGTGACGGCA AGGACGTGAG CCTGGAGGCT GGCCCTGGAC CCCCCGGCCT GCTTGCCCAC 
CCTGAGACTG CTGACACAGA TGGTGGATTG GAGTCCCCCC TGGGGCGCTG GGACTGCAGC 
TCGGTGACCT CCTTTGTCAC CGCTGTTGAG GCCTCAGGAG CTGAGGACCT AACTGGCCAC 
ACCTCCCCCT GGGCTGGTGT CCGACTCTCC CAGAGGGTGC CAAGGTCTTT GGGTACCCTA 
CAGCTGGCGC ATCAACCCCT CATGGAGGAC AGGGAGGCAG AACTAAATGC CCATCTGCAG 
GCCCTGACTC TGACCTTGCC AGATGATTCC CCCTCCCCCA AGTTCTTCCC AGACAGGAGC 
CCAACCCATA GTCCCCCTAG GGAACTGCTG CCTGGACCTT CTGACACCCA CATCACAAGA 
GAGGACCAGC TATCCCCCGA CGGTGATTTG GCTGCCCTCT GGCTGACAGA GGATGAGGCG 
AGCGCCACAG TGGGCAGGGA CCCCAGCCCC TCTGATCAGT GTCCACCGGA CCCTACCATG 
TCTGACCTGG AGCTGCTGCA AGGGCTCCGG GCGCTTGGCA AGAGCCCGGG TCCCATCACA 
TCCTTCACCC GGCCACGCTA CCTCCGACGG CTGAAAGAAG CCCAGGCTGC TCCTAGCCCA 
GACTTTTCAG GGCACAGCCC AGAGCTGGCC GAAGCCCTGC GGACAGGCCG TATCCCAGAT 
GCCCAAGCCG ATGAAGATGC ACTTGCCCAG CAGTTCGAGC GGCCAGACCC CACCAGACGG 
TGGCGGGAGG GAGTCGTGAA GTCCAGCTTC ACATATCTGC TGCTGGACCC CAGGCCATCT 
TCTACGTGGG CAAGGGAACA CGGGCCCGGC CAGATGTTCA CCTCTGGGAG GCCCTTAGCC 
ACCGAAGACG GCCAGGAAAA CAGGCCTGTC CCAAGGTACG CCGGATCTTG GACATCTGGG 
CCACTGGTCG TGGCATTGTT TCCCTGCATT GCTTCCAGCA TGTGGTCCCT GCAGAGGCTT 
ACACTCGAGA AGCGTGTCTT GTGGATGCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002688624.2
CDS	971..2743
Translation	MWATMRRRSAWLGSLHCTKARRREPRGGAARAHKGAAGSWKPAGGVTRWLPQGDSRGMGPAVGLARRLRTALQEEELRSVEELLRRGADPNLVLEDGAAAMHLAARAQHLRSLCCLEALLRRGGDPNARSAEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGFLPLDLAEQQGHQNCARLLRELEARTRTPTRTWKGSRKPEPEPEPGGPGPCEKRLDTSPSGPPHVMLNSTTLGTGDGKDVSLEAGPGPPGLLAHPETADTDGGLESPLGRWDCSSVTSFVTAVEASGAEDLTGHTSPWAGVRLSQRVPRSLGTLQLAHQPLMEDREAELNAHLQALTLTLPDDSPSPKFFPDRSPTHSPPRELLPGPSDTHITREDQLSPDGDLAALWLTEDEASATVGRDPSPSDQCPPDPTMSDLELLQGLRALGKSPGPITSFTRPRYLRRLKEAQAAPSPDFSGHSPELAEALRTGRIPDAQADEDALAQQFERPDPTRRWREGVVKSSFTYLLLDPRPSSTWAREHGPGQMFTSGRPLATEDGQENRPVPRYAGSWTSGPLVVALFPCIASSMWSLQRLTLEKRVLWML

Target ORF information:

RefSeq Version	XM_002688578.5
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002688578.5

ORF Insert Sequence:

ATGTGGGCGA CAATGCGGCG CCGGTCAGCC TGGCTGGGGT CGCTGCACTG CACCAAGGCG 
CGGAGAAGGG AGCCGAGGGG CGGAGCGGCG CGTGCGCACA AGGGCGCCGC GGGAAGTTGG 
AAACCGGCAG GCGGCGTGAC CAGGTGGCTG CCCCAAGGGG ACAGCCGGGG CATGGGCCCA 
GCTGTAGGTT TGGCGCGGCG GCTGCGGACG GCGCTGCAGG AGGAGGAGTT GCGATCTGTG 
GAGGAGTTGC TGCGCCGCGG TGCCGACCCC AATCTGGTAC TCGAGGATGG CGCGGCGGCC 
ATGCACCTGG CGGCCAGAGC CCAGCACCTA CGGAGTCTGT GTTGCCTCGA GGCCCTGCTG 
CGCCGAGGCG GGGACCCCAA CGCTCGATCG GCCGAGGCAC TGACACCGCT GCATGTGGCC 
GCTGCCTGGG GCTGTCGCCG GGGCCTGGAA CTGCTGCTGA GCCAGGGAGC GGACCCCGCG 
CTCCGCGACC AGGACGGATT CCTGCCTCTG GACCTGGCGG AGCAGCAGGG GCACCAGAAC 
TGTGCCCGTC TGCTTCGGGA GCTGGAGGCT CGGACCAGGA CCCCAACCCG GACCTGGAAA 
GGCTCCCGGA AGCCAGAACC CGAGCCAGAG CCTGGAGGCC CAGGCCCTTG TGAAAAGAGG 
CTGGATACCA GCCCCTCCGG TCCTCCTCAT GTGATGCTGA ACTCTACAAC ACTGGGCACA 
GGTGACGGCA AGGACGTGAG CCTGGAGGCT GGCCCTGGAC CCCCCGGCCT GCTTGCCCAC 
CCTGAGACTG CTGACACAGA TGGTGGATTG GAGTCCCCCC TGGGGCGCTG GGACTGCAGC 
TCGGTGACCT CCTTTGTCAC CGCTGTTGAG GCCTCAGGAG CTGAGGACCT AACTGGCCAC 
ACCTCCCCCT GGGCTGGTGT CCGACTCTCC CAGAGGGTGC CAAGGTCTTT GGGTACCCTA 
CAGCTGGCGC ATCAACCCCT CATGGAGGAC AGGGAGGCAG AACTAAATGC CCATCTGCAG 
GCCCTGACTC TGACCTTGCC AGATGATTCC CCCTCCCCCA AGTTCTTCCC AGACAGGAGC 
CCAACCCATA GTCCCCCTAG GGAACTGCTG CCTGGACCTT CTGACACCCA CATCACAAGA 
GAGGACCAGC TATCCCCCGA CGGTGATTTG GCTGCCCTCT GGCTGACAGA GGATGAGGCG 
AGCGCCACAG TGGGCAGGGA CCCCAGCCCC TCTGATCAGT GTCCACCGGA CCCTACCATG 
TCTGACCTGG AGCTGCTGCA AGGGCTCCGG GCGCTTGGCA AGAGCCCGGG TCCCATCACA 
TCCTTCACCC GGCCACGCTA CCTCCGACGG CTGAAAGAAG CCCAGGCTGC TCCTAGCCCA 
GACTTTTCAG GGCACAGCCC AGAGCTGGCC GAAGCCCTGC GGACAGGCCG TATCCCAGAT 
GCCCAAGCCG ATGAAGATGC ACTTGCCCAG CAGTTCGAGC GGCCAGACCC CACCAGACGG 
TGGCGGGAGG GAGTCGTGAA GTCCAGCTTC ACATATCTGC TGCTGGACCC CAGGCCATCT 
TCTACGTGGG CAAGGGAACA CGGGCCCGGC CAGATGTTCA CCTCTGGGAG GCCCTTAGCC 
ACCGAAGACG GCCAGGAAAA CAGGCCTGTC CCAAGGTACG CCGGATCTTG GACATCTGGG 
CCACTGGTCG TGGCATTGTT TCCCTGCATT GCTTCCAGCA TGTGGTCCCT GCAGAGGCTT 
ACACTCGAGA AGCGTGTCTT GTGGATGCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE1 ( XM_005198818.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005198875.1 Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa05040
Clone ID Related Accession (Same CDS sequence)	XM_005198818.3 , XM_005208558.3 , XM_005208558.4
Accession Version	XM_005198818.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645005.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005198818.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCGA CAATGCGGCG CCGGTCAGCC TGGCTGGGGT CGCTGCACTG CACCAAGGCG 
CGGAGAAGGG AGCCGAGGGG CGGAGCGGCG CGTGCGCACA AGGGCGCCGC GGGAAGTTGG 
AAACCGGCAG GCGGCGTGAC CAGGTGGCTG CCCCAAGGGG ACAGCCGGGG CATGGGCCCA 
GCTGTAGGTT TGGCGCGGCG GCTGCGGACG GCGCTGCAGG AGGAGGAGTT GCGATCTGTG 
GAGGAGTTGC TGCGCCGCGG TGCCGACCCC AATCTGGTAC TCGAGGATGG CGCGGCGGCC 
ATGCACCTGG CGGCCAGAGC CCAGCACCTA CGGAGTCTGT GTTGCCTCGA GGCCCTGCTG 
CGCCGAGGCG GGGACCCCAA CGCTCGATCG GCCGAGGCAC TGACACCGCT GCATGTGGCC 
GCTGCCTGGG GCTGTCGCCG GGGCCTGGAA CTGCTGCTGA GCCAGGGAGC GGACCCCGCG 
CTCCGCGACC AGGACGGATT CCTGCCTCTG GACCTGGCGG AGCAGCAGGG GCACCAGAAC 
TGTGCCCGTC TGCTTCGGGA GCTGGAGGCT CGGACCAGGA CCCCAACCCG GACCTGGAAA 
GGCTCCCGGA AGCCAGAACC CGAGCCAGAG CCTGGAGGCC CAGGCCCTTG TGAAAAGAGG 
CTGGATACCA GCCCCTCCGG TCCTCCTCAT GTGATGCTGA ACTCTACAAC ACTGGGCACA 
GGTGACGGCA AGGACGTGAG CCTGGAGGCT GGCCCTGGAC CCCCCGGCCT GCTTGCCCAC 
CCTGAGACTG CTGACACAGA TGGTGGATTG GAGTCCCCCC TGGGGCGCTG GGACTGCAGC 
TCGGTGACCT CCTTTGTCAC CGCTGTTGAG GCCTCAGGAG CTGAGGACCT AACTGGCCAC 
ACCTCCCCCT GGGCTGGTGT CCGACTCTCC CAGAGGGTGC CAAGGTCTTT GGGTACCCTA 
CAGCTGGCGC ATCAACCCCT CATGGAGGAC AGGGAGGCAG AACTAAATGC CCATCTGCAG 
GCCCTGACTC TGACCTTGCC AGATGATTCC CCCTCCCCCA AGTTCTTCCC AGACAGGAGC 
CCAACCCATA GTCCCCCTAG GGAACTGCTG CCTGGACCTT CTGACACCCA CATCACAAGA 
GAGGACCAGC TATCCCCCGA CGGTGATTTG GCTGCCCTCT GGCTGACAGA GGATGAGGCG 
AGCGCCACAG TGGGCAGGGA CCCCAGCCCC TCTGATCAGT GTCCACCGGA CCCTACCATG 
TCTGACCTGG AGCTGCTGCA AGGGCTCCGG GCGCTTGGCA AGAGCCCGGG TCCCATCACA 
TCCTTCACCC GGCCACGCTA CCTCCGACGG CTGAAAGAAG CCCAGGCTGC TCCTAGCCCA 
GACTTTTCAG GGCACAGCCC AGAGCTGGCC GAAGCCCTGC GGACAGGCCG TATCCCAGAT 
GCCCAAGCCG ATGAAGATGC ACTTGCCCAG CAGTTCGAGC GGCCAGACCC CACCAGACGG 
TGGCGGGAGG GAGTCGTGAA GTCCAGCTTC ACATATCTGC TGCTGGACCC CAGGAAGACT 
CAGGACCTGC CAGCCCGAGC CTTCTCAATG ACTCTGGCTG AATGCCTTCG GATTTTTGTC 
CAGGCCATCT TCTACGTGGG CAAGGGAACA CGGGCCCGGC CAGATGTTCA CCTCTGGGAG 
GCCCTTAGCC ACCGAAGACG GCCAGGAAAA CAGGCCTGTC CCAAGGTACG CCGGATCTTG 
GACATCTGGG CCACTGGTCG TGGCATTGTT TCCCTGCATT GCTTCCAGCA TGTGGTCCCT 
GCAGAGGCTT ACACTCGAGA AGCGTGTCTT GTGGATGCTC TAGGAATCCA GATGCTGACT 
AACCAGAAGC AAGGACACTG CTATGGAGTG GTGGCTGACT GGTCCCCGAC CCGGCGCCGC 
CGCCTGGGGG TACATCTGCT GCATCGTGCC CTCCTTGTCT TTCTGGCTGA GGGTGAGCGA 
GAGCTACGGC CCCAGGACAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005198875.1
CDS	971..2974
Translation	MWATMRRRSAWLGSLHCTKARRREPRGGAARAHKGAAGSWKPAGGVTRWLPQGDSRGMGPAVGLARRLRTALQEEELRSVEELLRRGADPNLVLEDGAAAMHLAARAQHLRSLCCLEALLRRGGDPNARSAEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGFLPLDLAEQQGHQNCARLLRELEARTRTPTRTWKGSRKPEPEPEPGGPGPCEKRLDTSPSGPPHVMLNSTTLGTGDGKDVSLEAGPGPPGLLAHPETADTDGGLESPLGRWDCSSVTSFVTAVEASGAEDLTGHTSPWAGVRLSQRVPRSLGTLQLAHQPLMEDREAELNAHLQALTLTLPDDSPSPKFFPDRSPTHSPPRELLPGPSDTHITREDQLSPDGDLAALWLTEDEASATVGRDPSPSDQCPPDPTMSDLELLQGLRALGKSPGPITSFTRPRYLRRLKEAQAAPSPDFSGHSPELAEALRTGRIPDAQADEDALAQQFERPDPTRRWREGVVKSSFTYLLLDPRKTQDLPARAFSMTLAECLRIFVQAIFYVGKGTRARPDVHLWEALSHRRRPGKQACPKVRRILDIWATGRGIVSLHCFQHVVPAEAYTREACLVDALGIQMLTNQKQGHCYGVVADWSPTRRRRLGVHLLHRALLVFLAEGERELRPQDI

Target ORF information:

RefSeq Version	XM_005198818.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005198818.3

ORF Insert Sequence:

ATGTGGGCGA CAATGCGGCG CCGGTCAGCC TGGCTGGGGT CGCTGCACTG CACCAAGGCG 
CGGAGAAGGG AGCCGAGGGG CGGAGCGGCG CGTGCGCACA AGGGCGCCGC GGGAAGTTGG 
AAACCGGCAG GCGGCGTGAC CAGGTGGCTG CCCCAAGGGG ACAGCCGGGG CATGGGCCCA 
GCTGTAGGTT TGGCGCGGCG GCTGCGGACG GCGCTGCAGG AGGAGGAGTT GCGATCTGTG 
GAGGAGTTGC TGCGCCGCGG TGCCGACCCC AATCTGGTAC TCGAGGATGG CGCGGCGGCC 
ATGCACCTGG CGGCCAGAGC CCAGCACCTA CGGAGTCTGT GTTGCCTCGA GGCCCTGCTG 
CGCCGAGGCG GGGACCCCAA CGCTCGATCG GCCGAGGCAC TGACACCGCT GCATGTGGCC 
GCTGCCTGGG GCTGTCGCCG GGGCCTGGAA CTGCTGCTGA GCCAGGGAGC GGACCCCGCG 
CTCCGCGACC AGGACGGATT CCTGCCTCTG GACCTGGCGG AGCAGCAGGG GCACCAGAAC 
TGTGCCCGTC TGCTTCGGGA GCTGGAGGCT CGGACCAGGA CCCCAACCCG GACCTGGAAA 
GGCTCCCGGA AGCCAGAACC CGAGCCAGAG CCTGGAGGCC CAGGCCCTTG TGAAAAGAGG 
CTGGATACCA GCCCCTCCGG TCCTCCTCAT GTGATGCTGA ACTCTACAAC ACTGGGCACA 
GGTGACGGCA AGGACGTGAG CCTGGAGGCT GGCCCTGGAC CCCCCGGCCT GCTTGCCCAC 
CCTGAGACTG CTGACACAGA TGGTGGATTG GAGTCCCCCC TGGGGCGCTG GGACTGCAGC 
TCGGTGACCT CCTTTGTCAC CGCTGTTGAG GCCTCAGGAG CTGAGGACCT AACTGGCCAC 
ACCTCCCCCT GGGCTGGTGT CCGACTCTCC CAGAGGGTGC CAAGGTCTTT GGGTACCCTA 
CAGCTGGCGC ATCAACCCCT CATGGAGGAC AGGGAGGCAG AACTAAATGC CCATCTGCAG 
GCCCTGACTC TGACCTTGCC AGATGATTCC CCCTCCCCCA AGTTCTTCCC AGACAGGAGC 
CCAACCCATA GTCCCCCTAG GGAACTGCTG CCTGGACCTT CTGACACCCA CATCACAAGA 
GAGGACCAGC TATCCCCCGA CGGTGATTTG GCTGCCCTCT GGCTGACAGA GGATGAGGCG 
AGCGCCACAG TGGGCAGGGA CCCCAGCCCC TCTGATCAGT GTCCACCGGA CCCTACCATG 
TCTGACCTGG AGCTGCTGCA AGGGCTCCGG GCGCTTGGCA AGAGCCCGGG TCCCATCACA 
TCCTTCACCC GGCCACGCTA CCTCCGACGG CTGAAAGAAG CCCAGGCTGC TCCTAGCCCA 
GACTTTTCAG GGCACAGCCC AGAGCTGGCC GAAGCCCTGC GGACAGGCCG TATCCCAGAT 
GCCCAAGCCG ATGAAGATGC ACTTGCCCAG CAGTTCGAGC GGCCAGACCC CACCAGACGG 
TGGCGGGAGG GAGTCGTGAA GTCCAGCTTC ACATATCTGC TGCTGGACCC CAGGAAGACT 
CAGGACCTGC CAGCCCGAGC CTTCTCAATG ACTCTGGCTG AATGCCTTCG GATTTTTGTC 
CAGGCCATCT TCTACGTGGG CAAGGGAACA CGGGCCCGGC CAGATGTTCA CCTCTGGGAG 
GCCCTTAGCC ACCGAAGACG GCCAGGAAAA CAGGCCTGTC CCAAGGTACG CCGGATCTTG 
GACATCTGGG CCACTGGTCG TGGCATTGTT TCCCTGCATT GCTTCCAGCA TGTGGTCCCT 
GCAGAGGCTT ACACTCGAGA AGCGTGTCTT GTGGATGCTC TAGGAATCCA GATGCTGACT 
AACCAGAAGC AAGGACACTG CTATGGAGTG GTGGCTGACT GGTCCCCGAC CCGGCGCCGC 
CGCCTGGGGG TACATCTGCT GCATCGTGCC CTCCTTGTCT TTCTGGCTGA GGGTGAGCGA 
GAGCTACGGC CCCAGGACAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE1 ( XM_005208558.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208615.1 Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa05040
Clone ID Related Accession (Same CDS sequence)	XM_005198818.3 , XM_005208558.3 , XM_005208558.4
Accession Version	XM_005208558.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208558.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCGA CAATGCGGCG CCGGTCAGCC TGGCTGGGGT CGCTGCACTG CACCAAGGCG 
CGGAGAAGGG AGCCGAGGGG CGGAGCGGCG CGTGCGCACA AGGGCGCCGC GGGAAGTTGG 
AAACCGGCAG GCGGCGTGAC CAGGTGGCTG CCCCAAGGGG ACAGCCGGGG CATGGGCCCA 
GCTGTAGGTT TGGCGCGGCG GCTGCGGACG GCGCTGCAGG AGGAGGAGTT GCGATCTGTG 
GAGGAGTTGC TGCGCCGCGG TGCCGACCCC AATCTGGTAC TCGAGGATGG CGCGGCGGCC 
ATGCACCTGG CGGCCAGAGC CCAGCACCTA CGGAGTCTGT GTTGCCTCGA GGCCCTGCTG 
CGCCGAGGCG GGGACCCCAA CGCTCGATCG GCCGAGGCAC TGACACCGCT GCATGTGGCC 
GCTGCCTGGG GCTGTCGCCG GGGCCTGGAA CTGCTGCTGA GCCAGGGAGC GGACCCCGCG 
CTCCGCGACC AGGACGGATT CCTGCCTCTG GACCTGGCGG AGCAGCAGGG GCACCAGAAC 
TGTGCCCGTC TGCTTCGGGA GCTGGAGGCT CGGACCAGGA CCCCAACCCG GACCTGGAAA 
GGCTCCCGGA AGCCAGAACC CGAGCCAGAG CCTGGAGGCC CAGGCCCTTG TGAAAAGAGG 
CTGGATACCA GCCCCTCCGG TCCTCCTCAT GTGATGCTGA ACTCTACAAC ACTGGGCACA 
GGTGACGGCA AGGACGTGAG CCTGGAGGCT GGCCCTGGAC CCCCCGGCCT GCTTGCCCAC 
CCTGAGACTG CTGACACAGA TGGTGGATTG GAGTCCCCCC TGGGGCGCTG GGACTGCAGC 
TCGGTGACCT CCTTTGTCAC CGCTGTTGAG GCCTCAGGAG CTGAGGACCT AACTGGCCAC 
ACCTCCCCCT GGGCTGGTGT CCGACTCTCC CAGAGGGTGC CAAGGTCTTT GGGTACCCTA 
CAGCTGGCGC ATCAACCCCT CATGGAGGAC AGGGAGGCAG AACTAAATGC CCATCTGCAG 
GCCCTGACTC TGACCTTGCC AGATGATTCC CCCTCCCCCA AGTTCTTCCC AGACAGGAGC 
CCAACCCATA GTCCCCCTAG GGAACTGCTG CCTGGACCTT CTGACACCCA CATCACAAGA 
GAGGACCAGC TATCCCCCGA CGGTGATTTG GCTGCCCTCT GGCTGACAGA GGATGAGGCG 
AGCGCCACAG TGGGCAGGGA CCCCAGCCCC TCTGATCAGT GTCCACCGGA CCCTACCATG 
TCTGACCTGG AGCTGCTGCA AGGGCTCCGG GCGCTTGGCA AGAGCCCGGG TCCCATCACA 
TCCTTCACCC GGCCACGCTA CCTCCGACGG CTGAAAGAAG CCCAGGCTGC TCCTAGCCCA 
GACTTTTCAG GGCACAGCCC AGAGCTGGCC GAAGCCCTGC GGACAGGCCG TATCCCAGAT 
GCCCAAGCCG ATGAAGATGC ACTTGCCCAG CAGTTCGAGC GGCCAGACCC CACCAGACGG 
TGGCGGGAGG GAGTCGTGAA GTCCAGCTTC ACATATCTGC TGCTGGACCC CAGGAAGACT 
CAGGACCTGC CAGCCCGAGC CTTCTCAATG ACTCTGGCTG AATGCCTTCG GATTTTTGTC 
CAGGCCATCT TCTACGTGGG CAAGGGAACA CGGGCCCGGC CAGATGTTCA CCTCTGGGAG 
GCCCTTAGCC ACCGAAGACG GCCAGGAAAA CAGGCCTGTC CCAAGGTACG CCGGATCTTG 
GACATCTGGG CCACTGGTCG TGGCATTGTT TCCCTGCATT GCTTCCAGCA TGTGGTCCCT 
GCAGAGGCTT ACACTCGAGA AGCGTGTCTT GTGGATGCTC TAGGAATCCA GATGCTGACT 
AACCAGAAGC AAGGACACTG CTATGGAGTG GTGGCTGACT GGTCCCCGAC CCGGCGCCGC 
CGCCTGGGGG TACATCTGCT GCATCGTGCC CTCCTTGTCT TTCTGGCTGA GGGTGAGCGA 
GAGCTACGGC CCCAGGACAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208615.1
CDS	971..2974
Translation	MWATMRRRSAWLGSLHCTKARRREPRGGAARAHKGAAGSWKPAGGVTRWLPQGDSRGMGPAVGLARRLRTALQEEELRSVEELLRRGADPNLVLEDGAAAMHLAARAQHLRSLCCLEALLRRGGDPNARSAEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGFLPLDLAEQQGHQNCARLLRELEARTRTPTRTWKGSRKPEPEPEPGGPGPCEKRLDTSPSGPPHVMLNSTTLGTGDGKDVSLEAGPGPPGLLAHPETADTDGGLESPLGRWDCSSVTSFVTAVEASGAEDLTGHTSPWAGVRLSQRVPRSLGTLQLAHQPLMEDREAELNAHLQALTLTLPDDSPSPKFFPDRSPTHSPPRELLPGPSDTHITREDQLSPDGDLAALWLTEDEASATVGRDPSPSDQCPPDPTMSDLELLQGLRALGKSPGPITSFTRPRYLRRLKEAQAAPSPDFSGHSPELAEALRTGRIPDAQADEDALAQQFERPDPTRRWREGVVKSSFTYLLLDPRKTQDLPARAFSMTLAECLRIFVQAIFYVGKGTRARPDVHLWEALSHRRRPGKQACPKVRRILDIWATGRGIVSLHCFQHVVPAEAYTREACLVDALGIQMLTNQKQGHCYGVVADWSPTRRRRLGVHLLHRALLVFLAEGERELRPQDI

Target ORF information:

RefSeq Version	XM_005208558.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208558.3

ORF Insert Sequence:

ATGTGGGCGA CAATGCGGCG CCGGTCAGCC TGGCTGGGGT CGCTGCACTG CACCAAGGCG 
CGGAGAAGGG AGCCGAGGGG CGGAGCGGCG CGTGCGCACA AGGGCGCCGC GGGAAGTTGG 
AAACCGGCAG GCGGCGTGAC CAGGTGGCTG CCCCAAGGGG ACAGCCGGGG CATGGGCCCA 
GCTGTAGGTT TGGCGCGGCG GCTGCGGACG GCGCTGCAGG AGGAGGAGTT GCGATCTGTG 
GAGGAGTTGC TGCGCCGCGG TGCCGACCCC AATCTGGTAC TCGAGGATGG CGCGGCGGCC 
ATGCACCTGG CGGCCAGAGC CCAGCACCTA CGGAGTCTGT GTTGCCTCGA GGCCCTGCTG 
CGCCGAGGCG GGGACCCCAA CGCTCGATCG GCCGAGGCAC TGACACCGCT GCATGTGGCC 
GCTGCCTGGG GCTGTCGCCG GGGCCTGGAA CTGCTGCTGA GCCAGGGAGC GGACCCCGCG 
CTCCGCGACC AGGACGGATT CCTGCCTCTG GACCTGGCGG AGCAGCAGGG GCACCAGAAC 
TGTGCCCGTC TGCTTCGGGA GCTGGAGGCT CGGACCAGGA CCCCAACCCG GACCTGGAAA 
GGCTCCCGGA AGCCAGAACC CGAGCCAGAG CCTGGAGGCC CAGGCCCTTG TGAAAAGAGG 
CTGGATACCA GCCCCTCCGG TCCTCCTCAT GTGATGCTGA ACTCTACAAC ACTGGGCACA 
GGTGACGGCA AGGACGTGAG CCTGGAGGCT GGCCCTGGAC CCCCCGGCCT GCTTGCCCAC 
CCTGAGACTG CTGACACAGA TGGTGGATTG GAGTCCCCCC TGGGGCGCTG GGACTGCAGC 
TCGGTGACCT CCTTTGTCAC CGCTGTTGAG GCCTCAGGAG CTGAGGACCT AACTGGCCAC 
ACCTCCCCCT GGGCTGGTGT CCGACTCTCC CAGAGGGTGC CAAGGTCTTT GGGTACCCTA 
CAGCTGGCGC ATCAACCCCT CATGGAGGAC AGGGAGGCAG AACTAAATGC CCATCTGCAG 
GCCCTGACTC TGACCTTGCC AGATGATTCC CCCTCCCCCA AGTTCTTCCC AGACAGGAGC 
CCAACCCATA GTCCCCCTAG GGAACTGCTG CCTGGACCTT CTGACACCCA CATCACAAGA 
GAGGACCAGC TATCCCCCGA CGGTGATTTG GCTGCCCTCT GGCTGACAGA GGATGAGGCG 
AGCGCCACAG TGGGCAGGGA CCCCAGCCCC TCTGATCAGT GTCCACCGGA CCCTACCATG 
TCTGACCTGG AGCTGCTGCA AGGGCTCCGG GCGCTTGGCA AGAGCCCGGG TCCCATCACA 
TCCTTCACCC GGCCACGCTA CCTCCGACGG CTGAAAGAAG CCCAGGCTGC TCCTAGCCCA 
GACTTTTCAG GGCACAGCCC AGAGCTGGCC GAAGCCCTGC GGACAGGCCG TATCCCAGAT 
GCCCAAGCCG ATGAAGATGC ACTTGCCCAG CAGTTCGAGC GGCCAGACCC CACCAGACGG 
TGGCGGGAGG GAGTCGTGAA GTCCAGCTTC ACATATCTGC TGCTGGACCC CAGGAAGACT 
CAGGACCTGC CAGCCCGAGC CTTCTCAATG ACTCTGGCTG AATGCCTTCG GATTTTTGTC 
CAGGCCATCT TCTACGTGGG CAAGGGAACA CGGGCCCGGC CAGATGTTCA CCTCTGGGAG 
GCCCTTAGCC ACCGAAGACG GCCAGGAAAA CAGGCCTGTC CCAAGGTACG CCGGATCTTG 
GACATCTGGG CCACTGGTCG TGGCATTGTT TCCCTGCATT GCTTCCAGCA TGTGGTCCCT 
GCAGAGGCTT ACACTCGAGA AGCGTGTCTT GTGGATGCTC TAGGAATCCA GATGCTGACT 
AACCAGAAGC AAGGACACTG CTATGGAGTG GTGGCTGACT GGTCCCCGAC CCGGCGCCGC 
CGCCTGGGGG TACATCTGCT GCATCGTGCC CTCCTTGTCT TTCTGGCTGA GGGTGAGCGA 
GAGCTACGGC CCCAGGACAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE1 ( XM_005208558.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208615.1 Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa05040
Clone ID Related Accession (Same CDS sequence)	XM_005198818.3 , XM_005208558.3 , XM_005208558.4
Accession Version	XM_005208558.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208558.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCGA CAATGCGGCG CCGGTCAGCC TGGCTGGGGT CGCTGCACTG CACCAAGGCG 
CGGAGAAGGG AGCCGAGGGG CGGAGCGGCG CGTGCGCACA AGGGCGCCGC GGGAAGTTGG 
AAACCGGCAG GCGGCGTGAC CAGGTGGCTG CCCCAAGGGG ACAGCCGGGG CATGGGCCCA 
GCTGTAGGTT TGGCGCGGCG GCTGCGGACG GCGCTGCAGG AGGAGGAGTT GCGATCTGTG 
GAGGAGTTGC TGCGCCGCGG TGCCGACCCC AATCTGGTAC TCGAGGATGG CGCGGCGGCC 
ATGCACCTGG CGGCCAGAGC CCAGCACCTA CGGAGTCTGT GTTGCCTCGA GGCCCTGCTG 
CGCCGAGGCG GGGACCCCAA CGCTCGATCG GCCGAGGCAC TGACACCGCT GCATGTGGCC 
GCTGCCTGGG GCTGTCGCCG GGGCCTGGAA CTGCTGCTGA GCCAGGGAGC GGACCCCGCG 
CTCCGCGACC AGGACGGATT CCTGCCTCTG GACCTGGCGG AGCAGCAGGG GCACCAGAAC 
TGTGCCCGTC TGCTTCGGGA GCTGGAGGCT CGGACCAGGA CCCCAACCCG GACCTGGAAA 
GGCTCCCGGA AGCCAGAACC CGAGCCAGAG CCTGGAGGCC CAGGCCCTTG TGAAAAGAGG 
CTGGATACCA GCCCCTCCGG TCCTCCTCAT GTGATGCTGA ACTCTACAAC ACTGGGCACA 
GGTGACGGCA AGGACGTGAG CCTGGAGGCT GGCCCTGGAC CCCCCGGCCT GCTTGCCCAC 
CCTGAGACTG CTGACACAGA TGGTGGATTG GAGTCCCCCC TGGGGCGCTG GGACTGCAGC 
TCGGTGACCT CCTTTGTCAC CGCTGTTGAG GCCTCAGGAG CTGAGGACCT AACTGGCCAC 
ACCTCCCCCT GGGCTGGTGT CCGACTCTCC CAGAGGGTGC CAAGGTCTTT GGGTACCCTA 
CAGCTGGCGC ATCAACCCCT CATGGAGGAC AGGGAGGCAG AACTAAATGC CCATCTGCAG 
GCCCTGACTC TGACCTTGCC AGATGATTCC CCCTCCCCCA AGTTCTTCCC AGACAGGAGC 
CCAACCCATA GTCCCCCTAG GGAACTGCTG CCTGGACCTT CTGACACCCA CATCACAAGA 
GAGGACCAGC TATCCCCCGA CGGTGATTTG GCTGCCCTCT GGCTGACAGA GGATGAGGCG 
AGCGCCACAG TGGGCAGGGA CCCCAGCCCC TCTGATCAGT GTCCACCGGA CCCTACCATG 
TCTGACCTGG AGCTGCTGCA AGGGCTCCGG GCGCTTGGCA AGAGCCCGGG TCCCATCACA 
TCCTTCACCC GGCCACGCTA CCTCCGACGG CTGAAAGAAG CCCAGGCTGC TCCTAGCCCA 
GACTTTTCAG GGCACAGCCC AGAGCTGGCC GAAGCCCTGC GGACAGGCCG TATCCCAGAT 
GCCCAAGCCG ATGAAGATGC ACTTGCCCAG CAGTTCGAGC GGCCAGACCC CACCAGACGG 
TGGCGGGAGG GAGTCGTGAA GTCCAGCTTC ACATATCTGC TGCTGGACCC CAGGAAGACT 
CAGGACCTGC CAGCCCGAGC CTTCTCAATG ACTCTGGCTG AATGCCTTCG GATTTTTGTC 
CAGGCCATCT TCTACGTGGG CAAGGGAACA CGGGCCCGGC CAGATGTTCA CCTCTGGGAG 
GCCCTTAGCC ACCGAAGACG GCCAGGAAAA CAGGCCTGTC CCAAGGTACG CCGGATCTTG 
GACATCTGGG CCACTGGTCG TGGCATTGTT TCCCTGCATT GCTTCCAGCA TGTGGTCCCT 
GCAGAGGCTT ACACTCGAGA AGCGTGTCTT GTGGATGCTC TAGGAATCCA GATGCTGACT 
AACCAGAAGC AAGGACACTG CTATGGAGTG GTGGCTGACT GGTCCCCGAC CCGGCGCCGC 
CGCCTGGGGG TACATCTGCT GCATCGTGCC CTCCTTGTCT TTCTGGCTGA GGGTGAGCGA 
GAGCTACGGC CCCAGGACAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208615.1
CDS	954..2957
Translation	MWATMRRRSAWLGSLHCTKARRREPRGGAARAHKGAAGSWKPAGGVTRWLPQGDSRGMGPAVGLARRLRTALQEEELRSVEELLRRGADPNLVLEDGAAAMHLAARAQHLRSLCCLEALLRRGGDPNARSAEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGFLPLDLAEQQGHQNCARLLRELEARTRTPTRTWKGSRKPEPEPEPGGPGPCEKRLDTSPSGPPHVMLNSTTLGTGDGKDVSLEAGPGPPGLLAHPETADTDGGLESPLGRWDCSSVTSFVTAVEASGAEDLTGHTSPWAGVRLSQRVPRSLGTLQLAHQPLMEDREAELNAHLQALTLTLPDDSPSPKFFPDRSPTHSPPRELLPGPSDTHITREDQLSPDGDLAALWLTEDEASATVGRDPSPSDQCPPDPTMSDLELLQGLRALGKSPGPITSFTRPRYLRRLKEAQAAPSPDFSGHSPELAEALRTGRIPDAQADEDALAQQFERPDPTRRWREGVVKSSFTYLLLDPRKTQDLPARAFSMTLAECLRIFVQAIFYVGKGTRARPDVHLWEALSHRRRPGKQACPKVRRILDIWATGRGIVSLHCFQHVVPAEAYTREACLVDALGIQMLTNQKQGHCYGVVADWSPTRRRRLGVHLLHRALLVFLAEGERELRPQDI

Target ORF information:

RefSeq Version	XM_005208558.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208558.4

ORF Insert Sequence:

ATGTGGGCGA CAATGCGGCG CCGGTCAGCC TGGCTGGGGT CGCTGCACTG CACCAAGGCG 
CGGAGAAGGG AGCCGAGGGG CGGAGCGGCG CGTGCGCACA AGGGCGCCGC GGGAAGTTGG 
AAACCGGCAG GCGGCGTGAC CAGGTGGCTG CCCCAAGGGG ACAGCCGGGG CATGGGCCCA 
GCTGTAGGTT TGGCGCGGCG GCTGCGGACG GCGCTGCAGG AGGAGGAGTT GCGATCTGTG 
GAGGAGTTGC TGCGCCGCGG TGCCGACCCC AATCTGGTAC TCGAGGATGG CGCGGCGGCC 
ATGCACCTGG CGGCCAGAGC CCAGCACCTA CGGAGTCTGT GTTGCCTCGA GGCCCTGCTG 
CGCCGAGGCG GGGACCCCAA CGCTCGATCG GCCGAGGCAC TGACACCGCT GCATGTGGCC 
GCTGCCTGGG GCTGTCGCCG GGGCCTGGAA CTGCTGCTGA GCCAGGGAGC GGACCCCGCG 
CTCCGCGACC AGGACGGATT CCTGCCTCTG GACCTGGCGG AGCAGCAGGG GCACCAGAAC 
TGTGCCCGTC TGCTTCGGGA GCTGGAGGCT CGGACCAGGA CCCCAACCCG GACCTGGAAA 
GGCTCCCGGA AGCCAGAACC CGAGCCAGAG CCTGGAGGCC CAGGCCCTTG TGAAAAGAGG 
CTGGATACCA GCCCCTCCGG TCCTCCTCAT GTGATGCTGA ACTCTACAAC ACTGGGCACA 
GGTGACGGCA AGGACGTGAG CCTGGAGGCT GGCCCTGGAC CCCCCGGCCT GCTTGCCCAC 
CCTGAGACTG CTGACACAGA TGGTGGATTG GAGTCCCCCC TGGGGCGCTG GGACTGCAGC 
TCGGTGACCT CCTTTGTCAC CGCTGTTGAG GCCTCAGGAG CTGAGGACCT AACTGGCCAC 
ACCTCCCCCT GGGCTGGTGT CCGACTCTCC CAGAGGGTGC CAAGGTCTTT GGGTACCCTA 
CAGCTGGCGC ATCAACCCCT CATGGAGGAC AGGGAGGCAG AACTAAATGC CCATCTGCAG 
GCCCTGACTC TGACCTTGCC AGATGATTCC CCCTCCCCCA AGTTCTTCCC AGACAGGAGC 
CCAACCCATA GTCCCCCTAG GGAACTGCTG CCTGGACCTT CTGACACCCA CATCACAAGA 
GAGGACCAGC TATCCCCCGA CGGTGATTTG GCTGCCCTCT GGCTGACAGA GGATGAGGCG 
AGCGCCACAG TGGGCAGGGA CCCCAGCCCC TCTGATCAGT GTCCACCGGA CCCTACCATG 
TCTGACCTGG AGCTGCTGCA AGGGCTCCGG GCGCTTGGCA AGAGCCCGGG TCCCATCACA 
TCCTTCACCC GGCCACGCTA CCTCCGACGG CTGAAAGAAG CCCAGGCTGC TCCTAGCCCA 
GACTTTTCAG GGCACAGCCC AGAGCTGGCC GAAGCCCTGC GGACAGGCCG TATCCCAGAT 
GCCCAAGCCG ATGAAGATGC ACTTGCCCAG CAGTTCGAGC GGCCAGACCC CACCAGACGG 
TGGCGGGAGG GAGTCGTGAA GTCCAGCTTC ACATATCTGC TGCTGGACCC CAGGAAGACT 
CAGGACCTGC CAGCCCGAGC CTTCTCAATG ACTCTGGCTG AATGCCTTCG GATTTTTGTC 
CAGGCCATCT TCTACGTGGG CAAGGGAACA CGGGCCCGGC CAGATGTTCA CCTCTGGGAG 
GCCCTTAGCC ACCGAAGACG GCCAGGAAAA CAGGCCTGTC CCAAGGTACG CCGGATCTTG 
GACATCTGGG CCACTGGTCG TGGCATTGTT TCCCTGCATT GCTTCCAGCA TGTGGTCCCT 
GCAGAGGCTT ACACTCGAGA AGCGTGTCTT GTGGATGCTC TAGGAATCCA GATGCTGACT 
AACCAGAAGC AAGGACACTG CTATGGAGTG GTGGCTGACT GGTCCCCGAC CCGGCGCCGC 
CGCCTGGGGG TACATCTGCT GCATCGTGCC CTCCTTGTCT TTCTGGCTGA GGGTGAGCGA 
GAGCTACGGC CCCAGGACAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ANKLE1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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