MAP3K5 cDNA ORF clone, Bos taurus(cattle)

The following MAP3K5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa216338 NM_001144081.2
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 5 (MAP3K5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa237865 XM_024996767.1
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa37311 NM_001144081.1
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 5 (MAP3K5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa216338
    Clone ID Related Accession (Same CDS sequence) NM_001144081.2
    Accession Version NM_001144081.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 5
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000009.1. On Jun 30, 2017 this sequence version replaced NM_001144081.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5721697.397.1, SRR6764281.35.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGCGCGG AGGCGGGCGA GGGCATCACT TTCTCCGTGC CACCCTTCGC CGAGGGCGGC 
    AGCTACCGGA GGGGAGGAGC GGCGACGGCG GCCGAGGGCG AGGAGAACCA GCTGCCACCG
    CCGCCACCGG GCAGCTTCTG GAACGTGGAA AGTGCCGCCG CTCCCGGCGC CGGGTGTCCC
    AGTGCCACCT CCGGGAGCAG CGCCACCCGG TGCCGGGGCA ACTCTGGGGG CGGAGGCGGC
    CGACGGACCA CGGTGGCCTA TGTCATCAAC GAGGCGAGCC AGGGGCAGCT GGTGGTGGCC
    GAGAGCGAGG CCCTGCAGTG CCTGCGGGAG GCGTGCGAGA CGGTGGGCGC CGCCCTGGAG
    ACCCTGCATT TTGGGAAACT GGACTTTGGG GAAACTGCGG TGCTGGACCG ATTTTACAAT
    GCAGATATCG CCGTGGTGGA GATGAGTGAT GCCTTCCGAC AACCATCCTT ATTTTACCAC
    CTTGGAGTGA GAGAAAGTTT CAGCATGGCC AACAACATCA TCCTCTACTG TGATACTAAT
    TCAGACTCTC TGCAGTCGCT GAAGGAGATC ATTTGCCAGA AGAATACTAT GTGCACTGGA
    AACTACACAT TTATCCCTTA CATGATAACG CCGCACAGCA AGGTCTACTG CTGTGACAGC
    AGCTTCATGA AGGGCTTGAC TGAGCTCATG CAGCCGAACT TTGAGCTGCT TCTGGGACCC
    ATCTGCTTAC CTCTCGTGGA TCGTTTCATT CAGCTTTTGA AGGTGGCACA AGCAAGTTCC
    AGCCAGTATT TCCGGGAGTC TATACTCAAC GATATCAGGA AAGCTCGTAA CTTATATACT
    GGCAAAGAAT TGGCAGCTGA ATTGGCAAGA ATTCGACAGC GAGTGGATAA TATTGAAGTC
    TTGACAGCAG ATATTGTCAT AAATCTGTTA CTTTCATATA GAGATATCCA GGACTATGAT
    TCTATTGTGA AGCTGGTAGA GACTTTAGAA AAGCTTCCAA CGTTTGATTT GGCCTCCCAT
    CACCATGTGA AGTTTCATTA TGCCTTTGCA CTGAATAGGA GAAACCTCCC TGGAGATAGA
    GCAAAAGCTC TTGACATTAT GATTCCCCTG GTGCAAAATG AAGAGCAGGT TGCTTCAGAT
    ATGTATTGCC TCGTTGGTCG AATCTACAAA GATATGTTTT TGGACTCTAA TTTCATGGAC
    ACTGAAAGTA GAGACCATGG AGCTTCTTGG TTCAAAAAGG CATTTGAATC AGAGCCAACA
    CTACAGTCAG GAATTAATTA TGCGGTCCTC CTCCTGGCAT CTGGGCACCA GTTTGAATCT
    TCCTTTGAGC TCCGAAAAGT TGGGGTGAAG CTAAGTAGTC TTCTTGGTAA AAAGGGAAAC
    TTGGAAAAAC TCCAGAGCTA CTGGGAAGTT GGATTTTTCC TGGGGGCCAG TGTCCTGGCC
    AATGACCACT TGAGAGTCAT TCAAGCATCT GAAAAGCTTT TTAAACTGAA GACACCTGCA
    TGGTACCTCA AGTCTATCGT AGAGACAATT TTGATATATA AGCATTTTGT GAAACTGACT
    TCGGAACAGC CTGTGGCCAA ACAGGAACTT GTGGACTTCT GGATGGATTT CCTGGTCGAG
    GCCACAAAGA CAGATGTCAC TGTAGTTAGG TTTCCAGTAT TAATATTAGA ACCAACCAAA
    ATCTATCAAC CTTCGTATTT GTCTATCAAC AATGAAGTTG AGGAGAAGAC AATATCTATT
    TGGCACGTGC TTCCTGATGA CAAGAAAGGT ATACATGAGT GGAATTTTAG TGCTTCTTCT
    GTCAGGGGTG TGAGTATTTC TAAATTTGAA GAACGATGCT GTTTTCTGTA CGTGCTTCAC
    AATTCTGACG ATTTCCAAAT CTACTTCTGT ACAGAACTTC ACTGTAAAAA GTTTTTTGAA
    ATGGTGAACA CCATTACTGA AGAGAAGGGG AAAAACACAG AGGAAGGAGA TTGTGAAGGT
    GACTCCCTGG AGTATGACTA TGAATACGAT GAAAATGGTG ACAGAGTCGT TTTAGGAAAA
    GGCACTTATG GGATAGTTTA TGCAGGTCGA GATCTGAGCA ACCAAGTCAG AATCGCCATT
    AAGGAAATCC CAGAGAGAGA CAGCAGATAC TCCCAGCCCC TGCATGAAGA AATAGCCTTG
    CATAAGCATC TGAAGCACAA AAATATTGTC CAGTATCTGG GCTCTTTCAG TGAAAACGGT
    TTCATTAAAA TCTTCATGGA GCAGGTCCCA GGAGGAAGTC TTTCTGCTCT CCTTCGTTCC
    AAATGGGGCC CACTGAAAGA CAATGAGCAA ACAATTGGCT TTTATACAAA GCAAATACTG
    GAAGGATTAA AATACCTCCA TGATAATCAG ATAGTTCACC GGGATATAAA GGGTGACAAT
    GTGTTGATTA ATACCTACAG CGGTGTTCTC AAGATTTCTG ATTTCGGAAC ATCAAAGAGG
    CTTGCTGGCA TAAATCCATG TACTGAAACT TTTACTGGTA CCCTCCAGTA TATGGCACCA
    GAAATAATAG ATAAAGGACC AAGAGGCTAT GGAAAGGCAG CAGACATTTG GTCTCTGGGC
    TGCACGATCA TTGAAATGGC CACTGGAAAA CCACCCTTTT ATGAACTGGG GGAACCACAA
    GCAGCTATGT TCAAGGTGGG GATGTTTAAA GTCCACCCAG AGATCCCAGA GTCCATGTCA
    GCAGAGGCCA AGGCGTTCAT ATTGAAGTGT TTCGAACCAG ACCCTGACAA GAGAGACTGT
    GCTAACGACT TGCTTATGGA TGAGTTCTTA AAAGTTTCAA GCAAAAAGAA AAAGACACAA
    CCTAAGCTTT CAGCTCTTTC AGCTGGATCG AATGAGTACC TCAGGAGTAT CTCCTTGCCA
    GTGCCAGTCC TGGTGGAGGA CACGAGCAGC AGCAGTGAGT ACGGCTCAGT TTCCCCTGAC
    ACAGAGTTGA AGGTGGACCC CTTCTCTTTC AAAACAAGAG CCAAGTCCTG TGGAGAAAAG
    GATGTCAAAG GCATCCGGAC GCTGTTTCTG GGCATCCCTG ATGAAAATTT TGAAGACCAC
    AGTGCTCCAC CTTCTCCAGA AGAAAAAGAT TCCGGATTCT TTATGCTGAG GAAGGACAGT
    GAGAGACGGG CCACGCTTCA CAGGATTCTG ACGGAAGACC AAGACAAAGT TGTGAGAAAC
    TTAATGGAAT CCTTGGCTCA GGGGGCTGAA GAACCTAAAC TGAAATGGGA ACACATCACA
    ACTCTGGTCG CAAGCCTCAG AGAATTTGTG AGGTCCACGG ACCGGAAGAT CATAGCCACC
    ACCCTGTCCA AGCTGAAGCT GGAGCTGGAC TTCGACAGCC ACGGGATCAG TCAGGTCCAG
    GTGGTGCTCT TTGGTTTCCA AGATGCTGTC AATAAAGTTC TTCGGAATCA TAACATCAAG
    CCACACTGGA TGTTTGCCTT AGACAGTATC ATCCGAAAGG CGGTACAGAC AGCTATTACC
    ATTCTGGTTC CAGAACTGAG GCCGCATTTC AGCCTTGCAT CTGAGAGTGA TACTGCCGAT
    CAAGAAGACT TGGATGCAGA AGGTGACCAT GACGAACGGC CTTCAGATCA AACTGTCCGA
    AGACCTCATG CCGTCCTTGA AGACGCGGTG GCCACCTCAG GGGTGAGCAC GCTCAGTTCT
    ACTGTATCCC ATGACTCCCA GAGTGCTCAC CGATCACTGA ATGTACAGCT TGGAAGGATG
    AAAATAGAAA CAAATAGATT ACTGGAAGAA TTGGTTCAGA AAGAGAAAGA ATTACAAGCA
    CTCCTTCATC AAGCTATTGA AGAGAAAGAC CAAGAAATTA AACACCTGAA GCTTAAGTCC
    CAACCAATAG ATGTTCCTGG GTTGCCTGGG TGTCACTCCA GTTCTCCTGG CACAAACACT
    GAAGATTCGG AACTTACTGA CTGGCTGAGA GAGAACGGAG CTGATGAAGA CACTATAAGT
    CGGTTTTTGG CTGAGGATTA TTCATTAATG GATGTTCTCT ACTATGTTAC ACGTGATGAT
    TTAAAATGCC TGAGACTAAG GGGCGGGATG CTGTGCACAC TGTGGAAAGC CATCACTGAC
    TTCCGAGACA AACAGGCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137553.2
    CDS449..4549
    Translation

    Target ORF information:

    RefSeq Version NM_001144081.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 5 (MAP3K5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144081.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGCGCGG AGGCGGGCGA GGGCATCACT TTCTCCGTGC CACCCTTCGC CGAGGGCGGC 
    AGCTACCGGA GGGGAGGAGC GGCGACGGCG GCCGAGGGCG AGGAGAACCA GCTGCCACCG
    CCGCCACCGG GCAGCTTCTG GAACGTGGAA AGTGCCGCCG CTCCCGGCGC CGGGTGTCCC
    AGTGCCACCT CCGGGAGCAG CGCCACCCGG TGCCGGGGCA ACTCTGGGGG CGGAGGCGGC
    CGACGGACCA CGGTGGCCTA TGTCATCAAC GAGGCGAGCC AGGGGCAGCT GGTGGTGGCC
    GAGAGCGAGG CCCTGCAGTG CCTGCGGGAG GCGTGCGAGA CGGTGGGCGC CGCCCTGGAG
    ACCCTGCATT TTGGGAAACT GGACTTTGGG GAAACTGCGG TGCTGGACCG ATTTTACAAT
    GCAGATATCG CCGTGGTGGA GATGAGTGAT GCCTTCCGAC AACCATCCTT ATTTTACCAC
    CTTGGAGTGA GAGAAAGTTT CAGCATGGCC AACAACATCA TCCTCTACTG TGATACTAAT
    TCAGACTCTC TGCAGTCGCT GAAGGAGATC ATTTGCCAGA AGAATACTAT GTGCACTGGA
    AACTACACAT TTATCCCTTA CATGATAACG CCGCACAGCA AGGTCTACTG CTGTGACAGC
    AGCTTCATGA AGGGCTTGAC TGAGCTCATG CAGCCGAACT TTGAGCTGCT TCTGGGACCC
    ATCTGCTTAC CTCTCGTGGA TCGTTTCATT CAGCTTTTGA AGGTGGCACA AGCAAGTTCC
    AGCCAGTATT TCCGGGAGTC TATACTCAAC GATATCAGGA AAGCTCGTAA CTTATATACT
    GGCAAAGAAT TGGCAGCTGA ATTGGCAAGA ATTCGACAGC GAGTGGATAA TATTGAAGTC
    TTGACAGCAG ATATTGTCAT AAATCTGTTA CTTTCATATA GAGATATCCA GGACTATGAT
    TCTATTGTGA AGCTGGTAGA GACTTTAGAA AAGCTTCCAA CGTTTGATTT GGCCTCCCAT
    CACCATGTGA AGTTTCATTA TGCCTTTGCA CTGAATAGGA GAAACCTCCC TGGAGATAGA
    GCAAAAGCTC TTGACATTAT GATTCCCCTG GTGCAAAATG AAGAGCAGGT TGCTTCAGAT
    ATGTATTGCC TCGTTGGTCG AATCTACAAA GATATGTTTT TGGACTCTAA TTTCATGGAC
    ACTGAAAGTA GAGACCATGG AGCTTCTTGG TTCAAAAAGG CATTTGAATC AGAGCCAACA
    CTACAGTCAG GAATTAATTA TGCGGTCCTC CTCCTGGCAT CTGGGCACCA GTTTGAATCT
    TCCTTTGAGC TCCGAAAAGT TGGGGTGAAG CTAAGTAGTC TTCTTGGTAA AAAGGGAAAC
    TTGGAAAAAC TCCAGAGCTA CTGGGAAGTT GGATTTTTCC TGGGGGCCAG TGTCCTGGCC
    AATGACCACT TGAGAGTCAT TCAAGCATCT GAAAAGCTTT TTAAACTGAA GACACCTGCA
    TGGTACCTCA AGTCTATCGT AGAGACAATT TTGATATATA AGCATTTTGT GAAACTGACT
    TCGGAACAGC CTGTGGCCAA ACAGGAACTT GTGGACTTCT GGATGGATTT CCTGGTCGAG
    GCCACAAAGA CAGATGTCAC TGTAGTTAGG TTTCCAGTAT TAATATTAGA ACCAACCAAA
    ATCTATCAAC CTTCGTATTT GTCTATCAAC AATGAAGTTG AGGAGAAGAC AATATCTATT
    TGGCACGTGC TTCCTGATGA CAAGAAAGGT ATACATGAGT GGAATTTTAG TGCTTCTTCT
    GTCAGGGGTG TGAGTATTTC TAAATTTGAA GAACGATGCT GTTTTCTGTA CGTGCTTCAC
    AATTCTGACG ATTTCCAAAT CTACTTCTGT ACAGAACTTC ACTGTAAAAA GTTTTTTGAA
    ATGGTGAACA CCATTACTGA AGAGAAGGGG AAAAACACAG AGGAAGGAGA TTGTGAAGGT
    GACTCCCTGG AGTATGACTA TGAATACGAT GAAAATGGTG ACAGAGTCGT TTTAGGAAAA
    GGCACTTATG GGATAGTTTA TGCAGGTCGA GATCTGAGCA ACCAAGTCAG AATCGCCATT
    AAGGAAATCC CAGAGAGAGA CAGCAGATAC TCCCAGCCCC TGCATGAAGA AATAGCCTTG
    CATAAGCATC TGAAGCACAA AAATATTGTC CAGTATCTGG GCTCTTTCAG TGAAAACGGT
    TTCATTAAAA TCTTCATGGA GCAGGTCCCA GGAGGAAGTC TTTCTGCTCT CCTTCGTTCC
    AAATGGGGCC CACTGAAAGA CAATGAGCAA ACAATTGGCT TTTATACAAA GCAAATACTG
    GAAGGATTAA AATACCTCCA TGATAATCAG ATAGTTCACC GGGATATAAA GGGTGACAAT
    GTGTTGATTA ATACCTACAG CGGTGTTCTC AAGATTTCTG ATTTCGGAAC ATCAAAGAGG
    CTTGCTGGCA TAAATCCATG TACTGAAACT TTTACTGGTA CCCTCCAGTA TATGGCACCA
    GAAATAATAG ATAAAGGACC AAGAGGCTAT GGAAAGGCAG CAGACATTTG GTCTCTGGGC
    TGCACGATCA TTGAAATGGC CACTGGAAAA CCACCCTTTT ATGAACTGGG GGAACCACAA
    GCAGCTATGT TCAAGGTGGG GATGTTTAAA GTCCACCCAG AGATCCCAGA GTCCATGTCA
    GCAGAGGCCA AGGCGTTCAT ATTGAAGTGT TTCGAACCAG ACCCTGACAA GAGAGACTGT
    GCTAACGACT TGCTTATGGA TGAGTTCTTA AAAGTTTCAA GCAAAAAGAA AAAGACACAA
    CCTAAGCTTT CAGCTCTTTC AGCTGGATCG AATGAGTACC TCAGGAGTAT CTCCTTGCCA
    GTGCCAGTCC TGGTGGAGGA CACGAGCAGC AGCAGTGAGT ACGGCTCAGT TTCCCCTGAC
    ACAGAGTTGA AGGTGGACCC CTTCTCTTTC AAAACAAGAG CCAAGTCCTG TGGAGAAAAG
    GATGTCAAAG GCATCCGGAC GCTGTTTCTG GGCATCCCTG ATGAAAATTT TGAAGACCAC
    AGTGCTCCAC CTTCTCCAGA AGAAAAAGAT TCCGGATTCT TTATGCTGAG GAAGGACAGT
    GAGAGACGGG CCACGCTTCA CAGGATTCTG ACGGAAGACC AAGACAAAGT TGTGAGAAAC
    TTAATGGAAT CCTTGGCTCA GGGGGCTGAA GAACCTAAAC TGAAATGGGA ACACATCACA
    ACTCTGGTCG CAAGCCTCAG AGAATTTGTG AGGTCCACGG ACCGGAAGAT CATAGCCACC
    ACCCTGTCCA AGCTGAAGCT GGAGCTGGAC TTCGACAGCC ACGGGATCAG TCAGGTCCAG
    GTGGTGCTCT TTGGTTTCCA AGATGCTGTC AATAAAGTTC TTCGGAATCA TAACATCAAG
    CCACACTGGA TGTTTGCCTT AGACAGTATC ATCCGAAAGG CGGTACAGAC AGCTATTACC
    ATTCTGGTTC CAGAACTGAG GCCGCATTTC AGCCTTGCAT CTGAGAGTGA TACTGCCGAT
    CAAGAAGACT TGGATGCAGA AGGTGACCAT GACGAACGGC CTTCAGATCA AACTGTCCGA
    AGACCTCATG CCGTCCTTGA AGACGCGGTG GCCACCTCAG GGGTGAGCAC GCTCAGTTCT
    ACTGTATCCC ATGACTCCCA GAGTGCTCAC CGATCACTGA ATGTACAGCT TGGAAGGATG
    AAAATAGAAA CAAATAGATT ACTGGAAGAA TTGGTTCAGA AAGAGAAAGA ATTACAAGCA
    CTCCTTCATC AAGCTATTGA AGAGAAAGAC CAAGAAATTA AACACCTGAA GCTTAAGTCC
    CAACCAATAG ATGTTCCTGG GTTGCCTGGG TGTCACTCCA GTTCTCCTGG CACAAACACT
    GAAGATTCGG AACTTACTGA CTGGCTGAGA GAGAACGGAG CTGATGAAGA CACTATAAGT
    CGGTTTTTGG CTGAGGATTA TTCATTAATG GATGTTCTCT ACTATGTTAC ACGTGATGAT
    TTAAAATGCC TGAGACTAAG GGGCGGGATG CTGTGCACAC TGTGGAAAGC CATCACTGAC
    TTCCGAGACA AACAGGCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237865
    Clone ID Related Accession (Same CDS sequence) XM_024996767.1
    Accession Version XM_024996767.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGATG CCTTCCGACA ACCATCCTTA TTTTACCACC TTGGAGTGAG AGAAAGTTTC 
    AGCATGGCCA ACAACATCAT CCTCTACTGT GATACTAATT CAGACTCTCT GCAGTCGCTG
    AAGGAGATCA TTTGCCAGAA GAATACTATG TGCACTGGAA ACTACACATT TATCCCTTAC
    ATGATAACGC CGCACAGCAA GGTCTACTGC TGTGACAGCA GCTTCATGAA GGGCTTGACT
    GAGCTCATGC AGCCGAACTT TGAGCTGCTT CTGGGACCCA TCTGCTTACC TCTCGTGGAT
    CGTTTCATTC AGCTTTTGAA GGTGGCACAA GCAAGTTCCA GCCAGTATTT CCGGGAGTCT
    ATACTCAACG ATATCAGGAA AGCTCGTAAC TTATATACTG GCAAAGAATT GGCAGCTGAA
    TTGGCAAGAA TTCGACAGCG AGTGGATAAT ATTGAAGTCT TGACAGCAGA TATTGTCATA
    AATCTGTTAC TTTCATATAG AGATATCCAG GACTATGATT CTATTGTGAA GCTGGTAGAG
    ACTTTAGAAA AGCTTCCAAC GTTTGATTTG GCCTCCCATC ACCATGTGAA GTTTCATTAT
    GCCTTTGCAC TGAATAGGAG AAACCTCCCT GGAGATAGAG CAAAAGCTCT TGACATTATG
    ATTCCCCTGG TGCAAAATGA AGAGCAGGTT GCTTCAGATA TGTATTGCCT CGTTGGTCGA
    ATCTACAAAG ATATGTTTTT GGACTCTAAT TTCATGGACA CTGAAAGTAG AGACCATGGA
    GCTTCTTGGT TCAAAAAGGC ATTTGAATCA GAGCCAACAC TACAGTCAGG AATTAATTAT
    GCGGTCCTCC TCCTGGCATC TGGGCACCAG TTTGAATCTT CCTTTGAGCT CCGAAAAGTT
    GGGGTGAAGC TAAGTAGTCT TCTTGGTAAA AAGGGAAACT TGGAAAAACT CCAGAGCTAC
    TGGGAAGTTG GATTTTTCCT GGGGGCCAGT GTCCTGGCCA ATGACCACTT GAGAGTCATT
    CAAGCATCTG AAAAGCTTTT TAAACTGAAG ACACCTGCAT GGTACCTCAA GTCTATCGTA
    GAGACAATTT TGATATATAA GCATTTTGTG AAACTGACTT CGGAACAGCC TGTGGCCAAA
    CAGGAACTTG TGGACTTCTG GATGGATTTC CTGGTCGAGG CCACAAAGAC AGATGTCACT
    GTAGTTAGGT TTCCAGTATT AATATTAGAA CCAACCAAAA TCTATCAACC TTCGTATTTG
    TCTATCAACA ATGAAGTTGA GGAGAAGACA ATATCTATTT GGCACGTGCT TCCTGATGAC
    AAGAAAGGTA TACATGAGTG GAATTTTAGT GCTTCTTCTG TCAGGGGTGT GAGTATTTCT
    AAATTTGAAG AACGATGCTG TTTTCTGTAC GTGCTTCACA ATTCTGACGA TTTCCAAATC
    TACTTCTGTA CAGAACTTCA CTGTAAAAAG TTTTTTGAAA TGGTGAACAC CATTACTGAA
    GAGAAGGGGA AAAACACAGA GGAAGGAGAT TGTGAAGGTG ACTCCCTGGA GTATGACTAT
    GAATACGATG AAAATGGTGA CAGAGTCGTT TTAGGAAAAG GCACTTATGG GATAGTTTAT
    GCAGGTCGAG ATCTGAGCAA CCAAGTCAGA ATCGCCATTA AGGAAATCCC AGAGAGAGAC
    AGCAGATACT CCCAGCCCCT GCATGAAGAA ATAGCCTTGC ATAAGCATCT GAAGCACAAA
    AATATTGTCC AGTATCTGGG CTCTTTCAGT GAAAACGGTT TCATTAAAAT CTTCATGGAG
    CAGGTCCCAG GAGGAAGTCT TTCTGCTCTC CTTCGTTCCA AATGGGGCCC ACTGAAAGAC
    AATGAGCAAA CAATTGGCTT TTATACAAAG CAAATACTGG AAGGATTAAA ATACCTCCAT
    GATAATCAGA TAGTTCACCG GGATATAAAG GGTGACAATG TGTTGATTAA TACCTACAGC
    GGTGTTCTCA AGATTTCTGA TTTCGGAACA TCAAAGAGGC TTGCTGGCAT AAATCCATGT
    ACTGAAACTT TTACTGGTAC CCTCCAGTAT ATGGCACCAG AAATAATAGA TAAAGGACCA
    AGAGGCTATG GAAAGGCAGC AGACATTTGG TCTCTGGGCT GCACGATCAT TGAAATGGCC
    ACTGGAAAAC CACCCTTTTA TGAACTGGGG GAACCACAAG CAGCTATGTT CAAGGTGGGG
    ATGTTTAAAG TCCACCCAGA GATCCCAGAG TCCATGTCAG CAGAGGCCAA GGCGTTCATA
    TTGAAGTGTT TCGAACCAGA CCCTGACAAG AGAGACTGTG CTAACGACTT GCTTATGGAT
    GAGTTCTTAA AAGTTTCAAG CAAAAAGAAA AAGACACAAC CTAAGCTTTC AGCTCTTTCA
    GCTGGATCGA ATGAGTACCT CAGGAGTATC TCCTTGCCAG TGCCAGTCCT GGTGGAGGAC
    ACGAGCAGCA GCAGTGAGTA CGGCTCAGTT TCCCCTGACA CAGAGTTGAA GGTGGACCCC
    TTCTCTTTCA AAACAAGAGC CAAGTCCTGT GGAGAAAAGG ATGTCAAAGG CATCCGGACG
    CTGTTTCTGG GCATCCCTGA TGAAAATTTT GAAGACCACA GTGCTCCACC TTCTCCAGAA
    GAAAAAGATT CCGGATTCTT TATGCTGAGG AAGGACAGTG AGAGACGGGC CACGCTTCAC
    AGGATTCTGA CGGAAGACCA AGACAAAGTT GTGAGAAACT TAATGGAATC CTTGGCTCAG
    GGGGCTGAAG AACCTAAACT GAAATGGGAA CACATCACAA CTCTGGTCGC AAGCCTCAGA
    GAATTTGTGA GGTCCACGGA CCGGAAGATC ATAGCCACCA CCCTGTCCAA GCTGAAGCTG
    GAGCTGGACT TCGACAGCCA CGGGATCAGT CAGGTCCAGG TGGTGCTCTT TGGTTTCCAA
    GATGCTGTCA ATAAAGTTCT TCGGAATCAT AACATCAAGC CACACTGGAT GTTTGCCTTA
    GACAGTATCA TCCGAAAGGC GGTACAGACA GCTATTACCA TTCTGGTTCC AGAACTGAGG
    CCGCATTTCA GCCTTGCATC TGAGAGTGAT ACTGCCGATC AAGAAGACTT GGATGCAGAA
    GGTGACCATG ACGAACGGCC TTCAGATCAA ACTGTCCGAA GACCTCATGC CGTCCTTGAA
    GACGCGGTGG CCACCTCAGG GGTGAGCACG CTCAGTTCTA CTGTATCCCA TGACTCCCAG
    AGTGCTCACC GATCACTGAA TGTACAGCTT GGAAGGATGA AAATAGAAAC AAATAGATTA
    CTGGAAGAAT TGGTTCAGAA AGAGAAAGAA TTACAAGCAC TCCTTCATCA AGCTATTGAA
    GAGAAAGACC AAGAAATTAA ACACCTGAAG CTTAAGTCCC AACCAATAGA TGTTCCTGGG
    TTGCCTGGGT GTCACTCCAG TTCTCCTGGC ACAAACACTG AAGATTCGGA ACTTACTGAC
    TGGCTGAGAG AGAACGGAGC TGATGAAGAC ACTATAAGTC GGTTTTTGGC TGAGGATTAT
    TCATTAATGG ATGTTCTCTA CTATGTTACA CGTGATGATT TAAAATGCCT GAGACTAAGG
    GGCGGGATGC TGTGCACACT GTGGAAAGCC ATCACTGACT TCCGAGACAA ACAGGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852535.1
    CDS157..3816
    Translation

    Target ORF information:

    RefSeq Version XM_024996767.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996767.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGATG CCTTCCGACA ACCATCCTTA TTTTACCACC TTGGAGTGAG AGAAAGTTTC 
    AGCATGGCCA ACAACATCAT CCTCTACTGT GATACTAATT CAGACTCTCT GCAGTCGCTG
    AAGGAGATCA TTTGCCAGAA GAATACTATG TGCACTGGAA ACTACACATT TATCCCTTAC
    ATGATAACGC CGCACAGCAA GGTCTACTGC TGTGACAGCA GCTTCATGAA GGGCTTGACT
    GAGCTCATGC AGCCGAACTT TGAGCTGCTT CTGGGACCCA TCTGCTTACC TCTCGTGGAT
    CGTTTCATTC AGCTTTTGAA GGTGGCACAA GCAAGTTCCA GCCAGTATTT CCGGGAGTCT
    ATACTCAACG ATATCAGGAA AGCTCGTAAC TTATATACTG GCAAAGAATT GGCAGCTGAA
    TTGGCAAGAA TTCGACAGCG AGTGGATAAT ATTGAAGTCT TGACAGCAGA TATTGTCATA
    AATCTGTTAC TTTCATATAG AGATATCCAG GACTATGATT CTATTGTGAA GCTGGTAGAG
    ACTTTAGAAA AGCTTCCAAC GTTTGATTTG GCCTCCCATC ACCATGTGAA GTTTCATTAT
    GCCTTTGCAC TGAATAGGAG AAACCTCCCT GGAGATAGAG CAAAAGCTCT TGACATTATG
    ATTCCCCTGG TGCAAAATGA AGAGCAGGTT GCTTCAGATA TGTATTGCCT CGTTGGTCGA
    ATCTACAAAG ATATGTTTTT GGACTCTAAT TTCATGGACA CTGAAAGTAG AGACCATGGA
    GCTTCTTGGT TCAAAAAGGC ATTTGAATCA GAGCCAACAC TACAGTCAGG AATTAATTAT
    GCGGTCCTCC TCCTGGCATC TGGGCACCAG TTTGAATCTT CCTTTGAGCT CCGAAAAGTT
    GGGGTGAAGC TAAGTAGTCT TCTTGGTAAA AAGGGAAACT TGGAAAAACT CCAGAGCTAC
    TGGGAAGTTG GATTTTTCCT GGGGGCCAGT GTCCTGGCCA ATGACCACTT GAGAGTCATT
    CAAGCATCTG AAAAGCTTTT TAAACTGAAG ACACCTGCAT GGTACCTCAA GTCTATCGTA
    GAGACAATTT TGATATATAA GCATTTTGTG AAACTGACTT CGGAACAGCC TGTGGCCAAA
    CAGGAACTTG TGGACTTCTG GATGGATTTC CTGGTCGAGG CCACAAAGAC AGATGTCACT
    GTAGTTAGGT TTCCAGTATT AATATTAGAA CCAACCAAAA TCTATCAACC TTCGTATTTG
    TCTATCAACA ATGAAGTTGA GGAGAAGACA ATATCTATTT GGCACGTGCT TCCTGATGAC
    AAGAAAGGTA TACATGAGTG GAATTTTAGT GCTTCTTCTG TCAGGGGTGT GAGTATTTCT
    AAATTTGAAG AACGATGCTG TTTTCTGTAC GTGCTTCACA ATTCTGACGA TTTCCAAATC
    TACTTCTGTA CAGAACTTCA CTGTAAAAAG TTTTTTGAAA TGGTGAACAC CATTACTGAA
    GAGAAGGGGA AAAACACAGA GGAAGGAGAT TGTGAAGGTG ACTCCCTGGA GTATGACTAT
    GAATACGATG AAAATGGTGA CAGAGTCGTT TTAGGAAAAG GCACTTATGG GATAGTTTAT
    GCAGGTCGAG ATCTGAGCAA CCAAGTCAGA ATCGCCATTA AGGAAATCCC AGAGAGAGAC
    AGCAGATACT CCCAGCCCCT GCATGAAGAA ATAGCCTTGC ATAAGCATCT GAAGCACAAA
    AATATTGTCC AGTATCTGGG CTCTTTCAGT GAAAACGGTT TCATTAAAAT CTTCATGGAG
    CAGGTCCCAG GAGGAAGTCT TTCTGCTCTC CTTCGTTCCA AATGGGGCCC ACTGAAAGAC
    AATGAGCAAA CAATTGGCTT TTATACAAAG CAAATACTGG AAGGATTAAA ATACCTCCAT
    GATAATCAGA TAGTTCACCG GGATATAAAG GGTGACAATG TGTTGATTAA TACCTACAGC
    GGTGTTCTCA AGATTTCTGA TTTCGGAACA TCAAAGAGGC TTGCTGGCAT AAATCCATGT
    ACTGAAACTT TTACTGGTAC CCTCCAGTAT ATGGCACCAG AAATAATAGA TAAAGGACCA
    AGAGGCTATG GAAAGGCAGC AGACATTTGG TCTCTGGGCT GCACGATCAT TGAAATGGCC
    ACTGGAAAAC CACCCTTTTA TGAACTGGGG GAACCACAAG CAGCTATGTT CAAGGTGGGG
    ATGTTTAAAG TCCACCCAGA GATCCCAGAG TCCATGTCAG CAGAGGCCAA GGCGTTCATA
    TTGAAGTGTT TCGAACCAGA CCCTGACAAG AGAGACTGTG CTAACGACTT GCTTATGGAT
    GAGTTCTTAA AAGTTTCAAG CAAAAAGAAA AAGACACAAC CTAAGCTTTC AGCTCTTTCA
    GCTGGATCGA ATGAGTACCT CAGGAGTATC TCCTTGCCAG TGCCAGTCCT GGTGGAGGAC
    ACGAGCAGCA GCAGTGAGTA CGGCTCAGTT TCCCCTGACA CAGAGTTGAA GGTGGACCCC
    TTCTCTTTCA AAACAAGAGC CAAGTCCTGT GGAGAAAAGG ATGTCAAAGG CATCCGGACG
    CTGTTTCTGG GCATCCCTGA TGAAAATTTT GAAGACCACA GTGCTCCACC TTCTCCAGAA
    GAAAAAGATT CCGGATTCTT TATGCTGAGG AAGGACAGTG AGAGACGGGC CACGCTTCAC
    AGGATTCTGA CGGAAGACCA AGACAAAGTT GTGAGAAACT TAATGGAATC CTTGGCTCAG
    GGGGCTGAAG AACCTAAACT GAAATGGGAA CACATCACAA CTCTGGTCGC AAGCCTCAGA
    GAATTTGTGA GGTCCACGGA CCGGAAGATC ATAGCCACCA CCCTGTCCAA GCTGAAGCTG
    GAGCTGGACT TCGACAGCCA CGGGATCAGT CAGGTCCAGG TGGTGCTCTT TGGTTTCCAA
    GATGCTGTCA ATAAAGTTCT TCGGAATCAT AACATCAAGC CACACTGGAT GTTTGCCTTA
    GACAGTATCA TCCGAAAGGC GGTACAGACA GCTATTACCA TTCTGGTTCC AGAACTGAGG
    CCGCATTTCA GCCTTGCATC TGAGAGTGAT ACTGCCGATC AAGAAGACTT GGATGCAGAA
    GGTGACCATG ACGAACGGCC TTCAGATCAA ACTGTCCGAA GACCTCATGC CGTCCTTGAA
    GACGCGGTGG CCACCTCAGG GGTGAGCACG CTCAGTTCTA CTGTATCCCA TGACTCCCAG
    AGTGCTCACC GATCACTGAA TGTACAGCTT GGAAGGATGA AAATAGAAAC AAATAGATTA
    CTGGAAGAAT TGGTTCAGAA AGAGAAAGAA TTACAAGCAC TCCTTCATCA AGCTATTGAA
    GAGAAAGACC AAGAAATTAA ACACCTGAAG CTTAAGTCCC AACCAATAGA TGTTCCTGGG
    TTGCCTGGGT GTCACTCCAG TTCTCCTGGC ACAAACACTG AAGATTCGGA ACTTACTGAC
    TGGCTGAGAG AGAACGGAGC TGATGAAGAC ACTATAAGTC GGTTTTTGGC TGAGGATTAT
    TCATTAATGG ATGTTCTCTA CTATGTTACA CGTGATGATT TAAAATGCCT GAGACTAAGG
    GGCGGGATGC TGTGCACACT GTGGAAAGCC ATCACTGACT TCCGAGACAA ACAGGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37311
    Clone ID Related Accession (Same CDS sequence) NM_001144081.1
    Accession Version NM_001144081.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product mitogen-activated protein kinase kinase kinase 5
    Comment INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AAFC03123160.1, AAFC03092977.1, AAFC03125465.1 and AAFC03026264.1. On Jan 22, 2009 this sequence version replaced XM_001788672.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGCGCGG AGGCGGGCGA GGGCATCACT TTCTCCGTGC CACCCTTCGC CGAGGGCGGC 
    AGCTACCGGA GGGGAGGAGC GGCGACGGCG GCCGAGGGCG AGGAGAACCA GCTGCCACCG
    CCGCCACCGG GCAGCTTCTG GAACGTGGAA AGTGCCGCCG CTCCCGGCGC CGGGTGTCCC
    AGTGCCACCT CCGGGAGCAG CGCCACCCGG TGCCGGGGCA ACTCTGGGGG CGGAGGCGGC
    CGACGGACCA CGGTGGCCTA TGTCATCAAC GAGGCGAGCC AGGGGCAGCT GGTGGTGGCC
    GAGAGCGAGG CCCTGCAGTG CCTGCGGGAG GCGTGCGAGA CGGTGGGCGC CGCCCTGGAG
    ACCCTGCATT TTGGGAAACT GGACTTTGGG GAAACTGCGG TGCTGGACCG ATTTTACAAT
    GCAGATATCG CCGTGGTGGA GATGAGTGAT GCCTTCCGAC AACCATCCTT ATTTTACCAC
    CTTGGAGTGA GAGAAAGTTT CAGCATGGCC AACAACATCA TCCTCTACTG TGATACTAAT
    TCAGACTCTC TGCAGTCGCT GAAGGAGATC ATTTGCCAGA AGAATACTAT GTGCACTGGA
    AACTACACAT TTATCCCTTA CATGATAACG CCGCACAGCA AGGTCTACTG CTGTGACAGC
    AGCTTCATGA AGGGCTTGAC TGAGCTCATG CAGCCGAACT TTGAGCTGCT TCTGGGACCC
    ATCTGCTTAC CTCTCGTGGA TCGTTTCATT CAGCTTTTGA AGGTGGCACA AGCAAGTTCC
    AGCCAGTATT TCCGGGAGTC TATACTCAAC GATATCAGGA AAGCTCGTAA CTTATATACT
    GGCAAAGAAT TGGCAGCTGA ATTGGCAAGA ATTCGACAGC GAGTGGATAA TATTGAAGTC
    TTGACAGCAG ATATTGTCAT AAATCTGTTA CTTTCATATA GAGATATCCA GGACTATGAT
    TCTATTGTGA AGCTGGTAGA GACTTTAGAA AAGCTTCCAA CGTTTGATTT GGCCTCCCAT
    CACCATGTGA AGTTTCATTA TGCCTTTGCA CTGAATAGGA GAAACCTCCC TGGAGATAGA
    GCAAAAGCTC TTGACATTAT GATTCCCCTG GTGCAAAATG AAGAGCAGGT TGCTTCAGAT
    ATGTATTGCC TCGTTGGTCG AATCTACAAA GATATGTTTT TGGACTCTAA TTTCATGGAC
    ACTGAAAGTA GAGACCATGG AGCTTCTTGG TTCAAAAAGG CATTTGAATC AGAGCCAACA
    CTACAGTCAG GAATTAATTA TGCGGTCCTC CTCCTGGCAT CTGGGCACCA GTTTGAATCT
    TCCTTTGAGC TCCGAAAAGT TGGGGTGAAG CTAAGTAGTC TTCTTGGTAA AAAGGGAAAC
    TTGGAAAAAC TCCAGAGCTA CTGGGAAGTT GGATTTTTCC TGGGGGCCAG TGTCCTGGCC
    AATGACCACT TGAGAGTCAT TCAAGCATCT GAAAAGCTTT TTAAACTGAA GACACCTGCA
    TGGTACCTCA AGTCTATCGT AGAGACAATT TTGATATATA AGCATTTTGT GAAACTGACT
    TCGGAACAGC CTGTGGCCAA ACAGGAACTT GTGGACTTCT GGATGGATTT CCTGGTCGAG
    GCCACAAAGA CAGATGTCAC TGTAGTTAGG TTTCCAGTAT TAATATTAGA ACCAACCAAA
    ATCTATCAAC CTTCGTATTT GTCTATCAAC AATGAAGTTG AGGAGAAGAC AATATGTATT
    TGGCACGTGC TTCCTGATGA CAAGAAAGGT ATACATGAGT GGAATTTTAG TGCTTCTTCT
    GTCAGGGGTG TGAGTATTTC TAAATTTGAA GAACGATGCT GTTTTCTGTA CGTGCTTCAC
    AATTCTGACG ATTTCCAAAT CTACTTCTGT ACAGAACTTC ACTGTAAAAA GTTTTTTGAA
    ATGGTGAACA CCATTACTGA AGAGAAGGGG AAAAACACAG AGGAAGGAGA TTGTGAAGGT
    GACTCCCTGG AGTATGACTA TGAATACGAT GAAAATGGTG ACAGAGTCGT TTTAGGAAAA
    GGCACTTATG GGATAGTTTA TGCAGGTCGA GATCTGAGCA ACCAAGTCAG AATCGCCATT
    AAGGAAATCC CAGAGAGAGA CAGCAGATAC TCCCAGCCCC TGCATGAAGA AATAGCCTTG
    CATAAGCATC TGAAGCACAA AAATATTGTC CAGTATCTGG GCTCTTTCAG TGAAAACGGT
    TTCATTAAAA TCTTCATGGA GCAGGTCCCA GGAGGAAGTC TTTCTGCTCT CCTTCGTTCC
    AAATGGGGCC CACTGAAAGA CAATGAGCAA ACAATTGGCT TTTATACAAA GCAAATACTG
    GAAGGATTAA AATACCTCCA TGATAATCAG ATAGTTCACC GGGATATAAA GGGTGACAAT
    GTGTTGATTA ATACCTACAG CGGTGTTCTC AAGATTTCTG ATTTCGGAAC ATCAAAGAGG
    CTTGCTGGCA TAAATCCATG TACTGAAACT TTTACTGGTA CCCTCCAGTA TATGGCACCA
    GAAATAATAG ATAAAGGACC AAGAGGCTAT GGAAAGGCAG CAGACATTTG GTCTCTGGGC
    TGCACGATCA TTGAAATGGC CACTGGAAAA CCACCCTTTT ATGAACTGGG GGAACCACAA
    GCAGCTATGT TCAAGGTGGG GATGTTTAAA GTCCACCCAG AGATCCCAGA GTCCATGTCA
    GCAGAGGCCA AGGCGTTCAT ATTGAAGTGT TTCGAACCAG ACCCTGACAA GAGAGACTGT
    GCTAACGACT TGCTTATGGA TGAGTTCTTA AAAGTTTCAA GCAAAAAGAA AAAGACACAA
    CCTAAGCTTT CAGCTCTTTC AGCTGGATCG AATGAGTACC TCAGGAGTAT CTCCTTGCCA
    GTGCCAGTCC TGGTGGAGGA CACGAGCAGC AGCAGTGAGT ACGGCTCAGT TTCCCCTGAC
    ACAGAGTTGA AGGTGGACCC CTTCTCTTTC AAAACAAGAG CCAAGTCCTG TGGAGAAAAG
    GATGTCAAAG GCATCCGGAC GCTGTTTCTG GGCATCCCTG ATGAAAATTT TGAAGACCAC
    AGTGCTCCAC CTTCTCCAGA AGAAAAAGAT TCCGGATTCT TTATGCTGAG GAAGGACAGT
    GAGAGACGGG CCACGCTTCA CAGGATTCTG ACGGAAGACC AAGACAAAGT TGTGAGAAAC
    TTAATGGAAT CCTTGGCTCA GGGGGCTGAA GAACCTAAAC TGAAATGGGA ACACATCACA
    ACTCTGGTCG CAAGCCTCAG AGAATTTGTG AGGTCCACGG ACCGGAAGAT CATAGCCACC
    ACCCTGTCCA AGCTGAAGCT GGAGCTGGAC TTCGACAGCC ACGGGATCAG TCAGGTCCAG
    GTGGTGCTCT TTGGTTTCCA AGATGCTGTC AATAAAGTTC TTCGGAATCA TAACATCAAG
    CCACACTGGA TGTTTGCCTT AGACAGTATC ATCCGAAAGG CGGTACAGAC AGCTATTACC
    ATTCTGGTTC CAGAACTGAG GCCGCATTTC AGCCTTGCAT CTGAGAGTGA TACTGCCGAT
    CAAGAAGACT TGGATGCAGA AGGTGACCAT GACGAACGGC CTTCAGATCA AACTGTCCGA
    AGACCTCATG CCGTCCTTGA AGACGCGGTG GCCACCTCAG GGGTGAGCAC GCTCAGTTCT
    ACTGTATCCC ATGACTCCCA GAGTGCTCAC CGATCACTGA ATGTACAGCT TGGAAGGATG
    AAAATAGAAA CAAATAGATT ACTGGAAGAA TTGGTTCAGA AAGAGAAAGA ATTACAAGCA
    CTCCTTCATC AAGCTATTGA AGAGAAAGAC CAAGAAATTA AACACCTGAA GCTTAAGTCC
    CAACCAATAG ATGTTCCTGG GTTGCCTGGG TGTCACTCCA GTTCTCCTGG CACAAACACT
    GAAGATTCGG AACTTACTGA CTGGCTGAGA GAGAACGGAG CTGATGAAGA CACTATAAGT
    CGGTTTTTGG CTGAGGATTA TTCATTAATG GATGTTCTCT ACTATGTTAC ACGTGATGAT
    TTAAAATGCC TGAGACTAAG GGGCGGGATG CTGTGCACAC TGTGGAAAGC CATCACTGAC
    TTCCGAGACA AACAGGCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137553.1
    CDS449..4549
    Translation

    Target ORF information:

    RefSeq Version NM_001144081.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 5 (MAP3K5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144081.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGCGCGG AGGCGGGCGA GGGCATCACT TTCTCCGTGC CACCCTTCGC CGAGGGCGGC 
    AGCTACCGGA GGGGAGGAGC GGCGACGGCG GCCGAGGGCG AGGAGAACCA GCTGCCACCG
    CCGCCACCGG GCAGCTTCTG GAACGTGGAA AGTGCCGCCG CTCCCGGCGC CGGGTGTCCC
    AGTGCCACCT CCGGGAGCAG CGCCACCCGG TGCCGGGGCA ACTCTGGGGG CGGAGGCGGC
    CGACGGACCA CGGTGGCCTA TGTCATCAAC GAGGCGAGCC AGGGGCAGCT GGTGGTGGCC
    GAGAGCGAGG CCCTGCAGTG CCTGCGGGAG GCGTGCGAGA CGGTGGGCGC CGCCCTGGAG
    ACCCTGCATT TTGGGAAACT GGACTTTGGG GAAACTGCGG TGCTGGACCG ATTTTACAAT
    GCAGATATCG CCGTGGTGGA GATGAGTGAT GCCTTCCGAC AACCATCCTT ATTTTACCAC
    CTTGGAGTGA GAGAAAGTTT CAGCATGGCC AACAACATCA TCCTCTACTG TGATACTAAT
    TCAGACTCTC TGCAGTCGCT GAAGGAGATC ATTTGCCAGA AGAATACTAT GTGCACTGGA
    AACTACACAT TTATCCCTTA CATGATAACG CCGCACAGCA AGGTCTACTG CTGTGACAGC
    AGCTTCATGA AGGGCTTGAC TGAGCTCATG CAGCCGAACT TTGAGCTGCT TCTGGGACCC
    ATCTGCTTAC CTCTCGTGGA TCGTTTCATT CAGCTTTTGA AGGTGGCACA AGCAAGTTCC
    AGCCAGTATT TCCGGGAGTC TATACTCAAC GATATCAGGA AAGCTCGTAA CTTATATACT
    GGCAAAGAAT TGGCAGCTGA ATTGGCAAGA ATTCGACAGC GAGTGGATAA TATTGAAGTC
    TTGACAGCAG ATATTGTCAT AAATCTGTTA CTTTCATATA GAGATATCCA GGACTATGAT
    TCTATTGTGA AGCTGGTAGA GACTTTAGAA AAGCTTCCAA CGTTTGATTT GGCCTCCCAT
    CACCATGTGA AGTTTCATTA TGCCTTTGCA CTGAATAGGA GAAACCTCCC TGGAGATAGA
    GCAAAAGCTC TTGACATTAT GATTCCCCTG GTGCAAAATG AAGAGCAGGT TGCTTCAGAT
    ATGTATTGCC TCGTTGGTCG AATCTACAAA GATATGTTTT TGGACTCTAA TTTCATGGAC
    ACTGAAAGTA GAGACCATGG AGCTTCTTGG TTCAAAAAGG CATTTGAATC AGAGCCAACA
    CTACAGTCAG GAATTAATTA TGCGGTCCTC CTCCTGGCAT CTGGGCACCA GTTTGAATCT
    TCCTTTGAGC TCCGAAAAGT TGGGGTGAAG CTAAGTAGTC TTCTTGGTAA AAAGGGAAAC
    TTGGAAAAAC TCCAGAGCTA CTGGGAAGTT GGATTTTTCC TGGGGGCCAG TGTCCTGGCC
    AATGACCACT TGAGAGTCAT TCAAGCATCT GAAAAGCTTT TTAAACTGAA GACACCTGCA
    TGGTACCTCA AGTCTATCGT AGAGACAATT TTGATATATA AGCATTTTGT GAAACTGACT
    TCGGAACAGC CTGTGGCCAA ACAGGAACTT GTGGACTTCT GGATGGATTT CCTGGTCGAG
    GCCACAAAGA CAGATGTCAC TGTAGTTAGG TTTCCAGTAT TAATATTAGA ACCAACCAAA
    ATCTATCAAC CTTCGTATTT GTCTATCAAC AATGAAGTTG AGGAGAAGAC AATATGTATT
    TGGCACGTGC TTCCTGATGA CAAGAAAGGT ATACATGAGT GGAATTTTAG TGCTTCTTCT
    GTCAGGGGTG TGAGTATTTC TAAATTTGAA GAACGATGCT GTTTTCTGTA CGTGCTTCAC
    AATTCTGACG ATTTCCAAAT CTACTTCTGT ACAGAACTTC ACTGTAAAAA GTTTTTTGAA
    ATGGTGAACA CCATTACTGA AGAGAAGGGG AAAAACACAG AGGAAGGAGA TTGTGAAGGT
    GACTCCCTGG AGTATGACTA TGAATACGAT GAAAATGGTG ACAGAGTCGT TTTAGGAAAA
    GGCACTTATG GGATAGTTTA TGCAGGTCGA GATCTGAGCA ACCAAGTCAG AATCGCCATT
    AAGGAAATCC CAGAGAGAGA CAGCAGATAC TCCCAGCCCC TGCATGAAGA AATAGCCTTG
    CATAAGCATC TGAAGCACAA AAATATTGTC CAGTATCTGG GCTCTTTCAG TGAAAACGGT
    TTCATTAAAA TCTTCATGGA GCAGGTCCCA GGAGGAAGTC TTTCTGCTCT CCTTCGTTCC
    AAATGGGGCC CACTGAAAGA CAATGAGCAA ACAATTGGCT TTTATACAAA GCAAATACTG
    GAAGGATTAA AATACCTCCA TGATAATCAG ATAGTTCACC GGGATATAAA GGGTGACAAT
    GTGTTGATTA ATACCTACAG CGGTGTTCTC AAGATTTCTG ATTTCGGAAC ATCAAAGAGG
    CTTGCTGGCA TAAATCCATG TACTGAAACT TTTACTGGTA CCCTCCAGTA TATGGCACCA
    GAAATAATAG ATAAAGGACC AAGAGGCTAT GGAAAGGCAG CAGACATTTG GTCTCTGGGC
    TGCACGATCA TTGAAATGGC CACTGGAAAA CCACCCTTTT ATGAACTGGG GGAACCACAA
    GCAGCTATGT TCAAGGTGGG GATGTTTAAA GTCCACCCAG AGATCCCAGA GTCCATGTCA
    GCAGAGGCCA AGGCGTTCAT ATTGAAGTGT TTCGAACCAG ACCCTGACAA GAGAGACTGT
    GCTAACGACT TGCTTATGGA TGAGTTCTTA AAAGTTTCAA GCAAAAAGAA AAAGACACAA
    CCTAAGCTTT CAGCTCTTTC AGCTGGATCG AATGAGTACC TCAGGAGTAT CTCCTTGCCA
    GTGCCAGTCC TGGTGGAGGA CACGAGCAGC AGCAGTGAGT ACGGCTCAGT TTCCCCTGAC
    ACAGAGTTGA AGGTGGACCC CTTCTCTTTC AAAACAAGAG CCAAGTCCTG TGGAGAAAAG
    GATGTCAAAG GCATCCGGAC GCTGTTTCTG GGCATCCCTG ATGAAAATTT TGAAGACCAC
    AGTGCTCCAC CTTCTCCAGA AGAAAAAGAT TCCGGATTCT TTATGCTGAG GAAGGACAGT
    GAGAGACGGG CCACGCTTCA CAGGATTCTG ACGGAAGACC AAGACAAAGT TGTGAGAAAC
    TTAATGGAAT CCTTGGCTCA GGGGGCTGAA GAACCTAAAC TGAAATGGGA ACACATCACA
    ACTCTGGTCG CAAGCCTCAG AGAATTTGTG AGGTCCACGG ACCGGAAGAT CATAGCCACC
    ACCCTGTCCA AGCTGAAGCT GGAGCTGGAC TTCGACAGCC ACGGGATCAG TCAGGTCCAG
    GTGGTGCTCT TTGGTTTCCA AGATGCTGTC AATAAAGTTC TTCGGAATCA TAACATCAAG
    CCACACTGGA TGTTTGCCTT AGACAGTATC ATCCGAAAGG CGGTACAGAC AGCTATTACC
    ATTCTGGTTC CAGAACTGAG GCCGCATTTC AGCCTTGCAT CTGAGAGTGA TACTGCCGAT
    CAAGAAGACT TGGATGCAGA AGGTGACCAT GACGAACGGC CTTCAGATCA AACTGTCCGA
    AGACCTCATG CCGTCCTTGA AGACGCGGTG GCCACCTCAG GGGTGAGCAC GCTCAGTTCT
    ACTGTATCCC ATGACTCCCA GAGTGCTCAC CGATCACTGA ATGTACAGCT TGGAAGGATG
    AAAATAGAAA CAAATAGATT ACTGGAAGAA TTGGTTCAGA AAGAGAAAGA ATTACAAGCA
    CTCCTTCATC AAGCTATTGA AGAGAAAGAC CAAGAAATTA AACACCTGAA GCTTAAGTCC
    CAACCAATAG ATGTTCCTGG GTTGCCTGGG TGTCACTCCA GTTCTCCTGG CACAAACACT
    GAAGATTCGG AACTTACTGA CTGGCTGAGA GAGAACGGAG CTGATGAAGA CACTATAAGT
    CGGTTTTTGG CTGAGGATTA TTCATTAATG GATGTTCTCT ACTATGTTAC ACGTGATGAT
    TTAAAATGCC TGAGACTAAG GGGCGGGATG CTGTGCACAC TGTGGAAAGC CATCACTGAC
    TTCCGAGACA AACAGGCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.