RAPGEF4 cDNA ORF clone, Bos taurus(cattle)

The following RAPGEF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa16343 XM_010801909.2
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa16343 XM_015474945.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa16343 XM_015474946.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa16344 XM_005202345.3
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa53626 XM_010801907.3
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa53625 XM_015474949.2
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa87900 XM_015474948.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa87901 XM_010801910.2
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa87902 XM_015474949.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa40485 NM_001192902.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa53626 XM_010801907.2 Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa16343
    Clone ID Related Accession (Same CDS sequence) XM_015474945.1 , XM_015474946.1 , XM_010801909.2
    Accession Version XM_015474945.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTTCCTTC AGAGAAGATC CTCAGGGCTG GAAAAATTTT ACGAAACGCC
    ATTCTCTCTC GAGCACCTCA CATGATAAGA GATAGAAAAT ACCACCTAAA GACATACAGA
    CAGTGCTGTG TGGGGACCGA GCTGGTGGAC TGGATGCTGC AGCAGACTCC ATGTGTTCAC
    TCCCGAACGC AAGCTGTGGG CATGTGGCAA GTCCTGGTAG AAGACAGCGT TCTCAACCAT
    GTGGATCAGG AGCATCACTT CCAAGACAAA TATTTATTTT ATCGGTTTCT GGATGATGAG
    CATGAGGACG CCCCTTTGCC TACGGAGGAG GAAAAAAAGG AGTGTGATGA GGAGCTGCAG
    GACACCATGC TGCTGCTGTC GCAGATGGGC CCGGACGCCC ACATGAGGAT GGTCCTTCGC
    AAACCACCTG GACAGAGGAC CATGGATGAC CTAGAAATTA TCTATGAAGA ACTCCTTCAC
    ATTAAAGCCT TATCCCATCT TTCTACCACA GTGAAACGGG AGTTGGCAGC GGTTCTCATT
    TTTGAGTCTC ATGCCAAAGG AGGGACTGTG TTATTTAACC AAGGGGAAGA AGGCACCTCC
    TGGTACATTA TTCTAAAAGG ATCAGTGAAT GTAGTCATTT ATGGCAAGGG TGTGGTCTGT
    ACCCTGCATG AGGGCGATGA CTTTGGCAAG TTAGCCCTAG TGAATGATGC CCCAAGAGCT
    GCCTCCATCG TCTTGAGAGA AGATAACTGC CATTTCTTAA GAGTAGACAA GGAGGATTTC
    AACCGGATCC TGAGGGACGT TGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGGGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACGATAC GCCTTGAGCC AGCTTTAAAT GAAGCAACAG ATTCTGTTTT AAATGACTTT
    GTTATGATGC ACTGTGTTTT TATGCCAAAT ACCCAGCTTT GCCCAGCTCT GGTGGCCCAT
    TACCATGCAC AGCCTTCGCA AGGTACAGAA CAAGAGAAAA TGGATTATGC CCTCAACAAC
    AAGAGGCGAG TCATCCGCCT GGTTTTGCAG TGGGCTGCCA TGTATGGAGA CATCCTACAA
    GAGGATGACG TGGCCATGGC TTTCCTGGAG GAGTTTTATG TGTCTGTATC AGATGATGCC
    CGGATGCTTG CCGCCCTCAA GGAGCAACTG CCTGAGCTGG AGAAGATTGT CAAGCAAATT
    TCAGAAGATG CAAAGGCGCC ACAAAAGAAG CACAAAGTTC TTTTGCAACA GTTCAACACA
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGTGGCT CTGATGAAGT TCTGTTTAAA
    GTCTATTGCG TGGACCACAC CTACACAACC ATCCGGGTAC CGGTGGCCGC CTCGGTGAAG
    GAAGTCCTCA GCGCCGTTGC CGACAAACTG GGCTCCGGGG AAGGCCTGAT CATCGTCAAG
    ATGAGTTCCG GGGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACAACG
    CTCACCATTA ATGGACGTCT GTTTGCTTGC CCGCGAGAGC AATTCGATTC ACTGACTCCC
    TTGCCAGAAC AGGAGGGCCC AACTGTTGGA ACAATGGGAA CTTTCGAACT GATGAGCTCC
    AAAGATTTGG CCTACCAGAT GACAATTTAT GATTGGGAGC TCTTCAACTG TGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATCATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGGAGGTT TAATGAAATT CAGTTTTGGG TTGTCACTGA GATCTGCCTT
    TGTTCCCAGC CTAGCAAGCG TGTTCAGCTC TTAAAAAAAT TTATTAAGAT CGCAGCCCAC
    TGTAAGGAGT ACAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAATGTA
    GCTGTGAGCC GCCTGGCACT GACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCAGAGTTTG AAAGTTTAAT GGATCCTTCA AGAAACCACA GGGCCTACAG GCTGACAGTA
    GCCAAATTGG ACCCTCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC GTTCATTGAC AATCTCGTAA ACTTTGAAAA AATGCGAATG
    ATTGCAAATA CTGCCAGAAC AGTGAGATAC TGCAGGAGCC AACCCTTCAA TCCTGATGCA
    GCTCAAGCTA ATAAGAACCA CCAGGATGTC CGGAGTTACG TTCGGCAATT AAATGTGATC
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGACTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330431.1
    CDS106..2682
    Translation

    Target ORF information:

    RefSeq Version XM_015474945.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474945.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTTCCTTC AGAGAAGATC CTCAGGGCTG GAAAAATTTT ACGAAACGCC
    ATTCTCTCTC GAGCACCTCA CATGATAAGA GATAGAAAAT ACCACCTAAA GACATACAGA
    CAGTGCTGTG TGGGGACCGA GCTGGTGGAC TGGATGCTGC AGCAGACTCC ATGTGTTCAC
    TCCCGAACGC AAGCTGTGGG CATGTGGCAA GTCCTGGTAG AAGACAGCGT TCTCAACCAT
    GTGGATCAGG AGCATCACTT CCAAGACAAA TATTTATTTT ATCGGTTTCT GGATGATGAG
    CATGAGGACG CCCCTTTGCC TACGGAGGAG GAAAAAAAGG AGTGTGATGA GGAGCTGCAG
    GACACCATGC TGCTGCTGTC GCAGATGGGC CCGGACGCCC ACATGAGGAT GGTCCTTCGC
    AAACCACCTG GACAGAGGAC CATGGATGAC CTAGAAATTA TCTATGAAGA ACTCCTTCAC
    ATTAAAGCCT TATCCCATCT TTCTACCACA GTGAAACGGG AGTTGGCAGC GGTTCTCATT
    TTTGAGTCTC ATGCCAAAGG AGGGACTGTG TTATTTAACC AAGGGGAAGA AGGCACCTCC
    TGGTACATTA TTCTAAAAGG ATCAGTGAAT GTAGTCATTT ATGGCAAGGG TGTGGTCTGT
    ACCCTGCATG AGGGCGATGA CTTTGGCAAG TTAGCCCTAG TGAATGATGC CCCAAGAGCT
    GCCTCCATCG TCTTGAGAGA AGATAACTGC CATTTCTTAA GAGTAGACAA GGAGGATTTC
    AACCGGATCC TGAGGGACGT TGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGGGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACGATAC GCCTTGAGCC AGCTTTAAAT GAAGCAACAG ATTCTGTTTT AAATGACTTT
    GTTATGATGC ACTGTGTTTT TATGCCAAAT ACCCAGCTTT GCCCAGCTCT GGTGGCCCAT
    TACCATGCAC AGCCTTCGCA AGGTACAGAA CAAGAGAAAA TGGATTATGC CCTCAACAAC
    AAGAGGCGAG TCATCCGCCT GGTTTTGCAG TGGGCTGCCA TGTATGGAGA CATCCTACAA
    GAGGATGACG TGGCCATGGC TTTCCTGGAG GAGTTTTATG TGTCTGTATC AGATGATGCC
    CGGATGCTTG CCGCCCTCAA GGAGCAACTG CCTGAGCTGG AGAAGATTGT CAAGCAAATT
    TCAGAAGATG CAAAGGCGCC ACAAAAGAAG CACAAAGTTC TTTTGCAACA GTTCAACACA
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGTGGCT CTGATGAAGT TCTGTTTAAA
    GTCTATTGCG TGGACCACAC CTACACAACC ATCCGGGTAC CGGTGGCCGC CTCGGTGAAG
    GAAGTCCTCA GCGCCGTTGC CGACAAACTG GGCTCCGGGG AAGGCCTGAT CATCGTCAAG
    ATGAGTTCCG GGGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACAACG
    CTCACCATTA ATGGACGTCT GTTTGCTTGC CCGCGAGAGC AATTCGATTC ACTGACTCCC
    TTGCCAGAAC AGGAGGGCCC AACTGTTGGA ACAATGGGAA CTTTCGAACT GATGAGCTCC
    AAAGATTTGG CCTACCAGAT GACAATTTAT GATTGGGAGC TCTTCAACTG TGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATCATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGGAGGTT TAATGAAATT CAGTTTTGGG TTGTCACTGA GATCTGCCTT
    TGTTCCCAGC CTAGCAAGCG TGTTCAGCTC TTAAAAAAAT TTATTAAGAT CGCAGCCCAC
    TGTAAGGAGT ACAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAATGTA
    GCTGTGAGCC GCCTGGCACT GACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCAGAGTTTG AAAGTTTAAT GGATCCTTCA AGAAACCACA GGGCCTACAG GCTGACAGTA
    GCCAAATTGG ACCCTCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC GTTCATTGAC AATCTCGTAA ACTTTGAAAA AATGCGAATG
    ATTGCAAATA CTGCCAGAAC AGTGAGATAC TGCAGGAGCC AACCCTTCAA TCCTGATGCA
    GCTCAAGCTA ATAAGAACCA CCAGGATGTC CGGAGTTACG TTCGGCAATT AAATGTGATC
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGACTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16343
    Clone ID Related Accession (Same CDS sequence) XM_015474945.1 , XM_015474946.1 , XM_010801909.2
    Accession Version XM_015474946.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTTCCTTC AGAGAAGATC CTCAGGGCTG GAAAAATTTT ACGAAACGCC
    ATTCTCTCTC GAGCACCTCA CATGATAAGA GATAGAAAAT ACCACCTAAA GACATACAGA
    CAGTGCTGTG TGGGGACCGA GCTGGTGGAC TGGATGCTGC AGCAGACTCC ATGTGTTCAC
    TCCCGAACGC AAGCTGTGGG CATGTGGCAA GTCCTGGTAG AAGACAGCGT TCTCAACCAT
    GTGGATCAGG AGCATCACTT CCAAGACAAA TATTTATTTT ATCGGTTTCT GGATGATGAG
    CATGAGGACG CCCCTTTGCC TACGGAGGAG GAAAAAAAGG AGTGTGATGA GGAGCTGCAG
    GACACCATGC TGCTGCTGTC GCAGATGGGC CCGGACGCCC ACATGAGGAT GGTCCTTCGC
    AAACCACCTG GACAGAGGAC CATGGATGAC CTAGAAATTA TCTATGAAGA ACTCCTTCAC
    ATTAAAGCCT TATCCCATCT TTCTACCACA GTGAAACGGG AGTTGGCAGC GGTTCTCATT
    TTTGAGTCTC ATGCCAAAGG AGGGACTGTG TTATTTAACC AAGGGGAAGA AGGCACCTCC
    TGGTACATTA TTCTAAAAGG ATCAGTGAAT GTAGTCATTT ATGGCAAGGG TGTGGTCTGT
    ACCCTGCATG AGGGCGATGA CTTTGGCAAG TTAGCCCTAG TGAATGATGC CCCAAGAGCT
    GCCTCCATCG TCTTGAGAGA AGATAACTGC CATTTCTTAA GAGTAGACAA GGAGGATTTC
    AACCGGATCC TGAGGGACGT TGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGGGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACGATAC GCCTTGAGCC AGCTTTAAAT GAAGCAACAG ATTCTGTTTT AAATGACTTT
    GTTATGATGC ACTGTGTTTT TATGCCAAAT ACCCAGCTTT GCCCAGCTCT GGTGGCCCAT
    TACCATGCAC AGCCTTCGCA AGGTACAGAA CAAGAGAAAA TGGATTATGC CCTCAACAAC
    AAGAGGCGAG TCATCCGCCT GGTTTTGCAG TGGGCTGCCA TGTATGGAGA CATCCTACAA
    GAGGATGACG TGGCCATGGC TTTCCTGGAG GAGTTTTATG TGTCTGTATC AGATGATGCC
    CGGATGCTTG CCGCCCTCAA GGAGCAACTG CCTGAGCTGG AGAAGATTGT CAAGCAAATT
    TCAGAAGATG CAAAGGCGCC ACAAAAGAAG CACAAAGTTC TTTTGCAACA GTTCAACACA
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGTGGCT CTGATGAAGT TCTGTTTAAA
    GTCTATTGCG TGGACCACAC CTACACAACC ATCCGGGTAC CGGTGGCCGC CTCGGTGAAG
    GAAGTCCTCA GCGCCGTTGC CGACAAACTG GGCTCCGGGG AAGGCCTGAT CATCGTCAAG
    ATGAGTTCCG GGGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACAACG
    CTCACCATTA ATGGACGTCT GTTTGCTTGC CCGCGAGAGC AATTCGATTC ACTGACTCCC
    TTGCCAGAAC AGGAGGGCCC AACTGTTGGA ACAATGGGAA CTTTCGAACT GATGAGCTCC
    AAAGATTTGG CCTACCAGAT GACAATTTAT GATTGGGAGC TCTTCAACTG TGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATCATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGGAGGTT TAATGAAATT CAGTTTTGGG TTGTCACTGA GATCTGCCTT
    TGTTCCCAGC CTAGCAAGCG TGTTCAGCTC TTAAAAAAAT TTATTAAGAT CGCAGCCCAC
    TGTAAGGAGT ACAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAATGTA
    GCTGTGAGCC GCCTGGCACT GACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCAGAGTTTG AAAGTTTAAT GGATCCTTCA AGAAACCACA GGGCCTACAG GCTGACAGTA
    GCCAAATTGG ACCCTCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC GTTCATTGAC AATCTCGTAA ACTTTGAAAA AATGCGAATG
    ATTGCAAATA CTGCCAGAAC AGTGAGATAC TGCAGGAGCC AACCCTTCAA TCCTGATGCA
    GCTCAAGCTA ATAAGAACCA CCAGGATGTC CGGAGTTACG TTCGGCAATT AAATGTGATC
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGACTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330432.1
    CDS97..2673
    Translation

    Target ORF information:

    RefSeq Version XM_015474946.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474946.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTTCCTTC AGAGAAGATC CTCAGGGCTG GAAAAATTTT ACGAAACGCC
    ATTCTCTCTC GAGCACCTCA CATGATAAGA GATAGAAAAT ACCACCTAAA GACATACAGA
    CAGTGCTGTG TGGGGACCGA GCTGGTGGAC TGGATGCTGC AGCAGACTCC ATGTGTTCAC
    TCCCGAACGC AAGCTGTGGG CATGTGGCAA GTCCTGGTAG AAGACAGCGT TCTCAACCAT
    GTGGATCAGG AGCATCACTT CCAAGACAAA TATTTATTTT ATCGGTTTCT GGATGATGAG
    CATGAGGACG CCCCTTTGCC TACGGAGGAG GAAAAAAAGG AGTGTGATGA GGAGCTGCAG
    GACACCATGC TGCTGCTGTC GCAGATGGGC CCGGACGCCC ACATGAGGAT GGTCCTTCGC
    AAACCACCTG GACAGAGGAC CATGGATGAC CTAGAAATTA TCTATGAAGA ACTCCTTCAC
    ATTAAAGCCT TATCCCATCT TTCTACCACA GTGAAACGGG AGTTGGCAGC GGTTCTCATT
    TTTGAGTCTC ATGCCAAAGG AGGGACTGTG TTATTTAACC AAGGGGAAGA AGGCACCTCC
    TGGTACATTA TTCTAAAAGG ATCAGTGAAT GTAGTCATTT ATGGCAAGGG TGTGGTCTGT
    ACCCTGCATG AGGGCGATGA CTTTGGCAAG TTAGCCCTAG TGAATGATGC CCCAAGAGCT
    GCCTCCATCG TCTTGAGAGA AGATAACTGC CATTTCTTAA GAGTAGACAA GGAGGATTTC
    AACCGGATCC TGAGGGACGT TGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGGGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACGATAC GCCTTGAGCC AGCTTTAAAT GAAGCAACAG ATTCTGTTTT AAATGACTTT
    GTTATGATGC ACTGTGTTTT TATGCCAAAT ACCCAGCTTT GCCCAGCTCT GGTGGCCCAT
    TACCATGCAC AGCCTTCGCA AGGTACAGAA CAAGAGAAAA TGGATTATGC CCTCAACAAC
    AAGAGGCGAG TCATCCGCCT GGTTTTGCAG TGGGCTGCCA TGTATGGAGA CATCCTACAA
    GAGGATGACG TGGCCATGGC TTTCCTGGAG GAGTTTTATG TGTCTGTATC AGATGATGCC
    CGGATGCTTG CCGCCCTCAA GGAGCAACTG CCTGAGCTGG AGAAGATTGT CAAGCAAATT
    TCAGAAGATG CAAAGGCGCC ACAAAAGAAG CACAAAGTTC TTTTGCAACA GTTCAACACA
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGTGGCT CTGATGAAGT TCTGTTTAAA
    GTCTATTGCG TGGACCACAC CTACACAACC ATCCGGGTAC CGGTGGCCGC CTCGGTGAAG
    GAAGTCCTCA GCGCCGTTGC CGACAAACTG GGCTCCGGGG AAGGCCTGAT CATCGTCAAG
    ATGAGTTCCG GGGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACAACG
    CTCACCATTA ATGGACGTCT GTTTGCTTGC CCGCGAGAGC AATTCGATTC ACTGACTCCC
    TTGCCAGAAC AGGAGGGCCC AACTGTTGGA ACAATGGGAA CTTTCGAACT GATGAGCTCC
    AAAGATTTGG CCTACCAGAT GACAATTTAT GATTGGGAGC TCTTCAACTG TGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATCATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGGAGGTT TAATGAAATT CAGTTTTGGG TTGTCACTGA GATCTGCCTT
    TGTTCCCAGC CTAGCAAGCG TGTTCAGCTC TTAAAAAAAT TTATTAAGAT CGCAGCCCAC
    TGTAAGGAGT ACAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAATGTA
    GCTGTGAGCC GCCTGGCACT GACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCAGAGTTTG AAAGTTTAAT GGATCCTTCA AGAAACCACA GGGCCTACAG GCTGACAGTA
    GCCAAATTGG ACCCTCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC GTTCATTGAC AATCTCGTAA ACTTTGAAAA AATGCGAATG
    ATTGCAAATA CTGCCAGAAC AGTGAGATAC TGCAGGAGCC AACCCTTCAA TCCTGATGCA
    GCTCAAGCTA ATAAGAACCA CCAGGATGTC CGGAGTTACG TTCGGCAATT AAATGTGATC
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGACTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16343
    Clone ID Related Accession (Same CDS sequence) XM_015474945.1 , XM_015474946.1 , XM_010801909.2
    Accession Version XM_010801909.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTTCCTTC AGAGAAGATC CTCAGGGCTG GAAAAATTTT ACGAAACGCC
    ATTCTCTCTC GAGCACCTCA CATGATAAGA GATAGAAAAT ACCACCTAAA GACATACAGA
    CAGTGCTGTG TGGGGACCGA GCTGGTGGAC TGGATGCTGC AGCAGACTCC ATGTGTTCAC
    TCCCGAACGC AAGCTGTGGG CATGTGGCAA GTCCTGGTAG AAGACAGCGT TCTCAACCAT
    GTGGATCAGG AGCATCACTT CCAAGACAAA TATTTATTTT ATCGGTTTCT GGATGATGAG
    CATGAGGACG CCCCTTTGCC TACGGAGGAG GAAAAAAAGG AGTGTGATGA GGAGCTGCAG
    GACACCATGC TGCTGCTGTC GCAGATGGGC CCGGACGCCC ACATGAGGAT GGTCCTTCGC
    AAACCACCTG GACAGAGGAC CATGGATGAC CTAGAAATTA TCTATGAAGA ACTCCTTCAC
    ATTAAAGCCT TATCCCATCT TTCTACCACA GTGAAACGGG AGTTGGCAGC GGTTCTCATT
    TTTGAGTCTC ATGCCAAAGG AGGGACTGTG TTATTTAACC AAGGGGAAGA AGGCACCTCC
    TGGTACATTA TTCTAAAAGG ATCAGTGAAT GTAGTCATTT ATGGCAAGGG TGTGGTCTGT
    ACCCTGCATG AGGGCGATGA CTTTGGCAAG TTAGCCCTAG TGAATGATGC CCCAAGAGCT
    GCCTCCATCG TCTTGAGAGA AGATAACTGC CATTTCTTAA GAGTAGACAA GGAGGATTTC
    AACCGGATCC TGAGGGACGT TGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGGGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACGATAC GCCTTGAGCC AGCTTTAAAT GAAGCAACAG ATTCTGTTTT AAATGACTTT
    GTTATGATGC ACTGTGTTTT TATGCCAAAT ACCCAGCTTT GCCCAGCTCT GGTGGCCCAT
    TACCATGCAC AGCCTTCGCA AGGTACAGAA CAAGAGAAAA TGGATTATGC CCTCAACAAC
    AAGAGGCGAG TCATCCGCCT GGTTTTGCAG TGGGCTGCCA TGTATGGAGA CATCCTACAA
    GAGGATGACG TGGCCATGGC TTTCCTGGAG GAGTTTTATG TGTCTGTATC AGATGATGCC
    CGGATGCTTG CCGCCCTCAA GGAGCAACTG CCTGAGCTGG AGAAGATTGT CAAGCAAATT
    TCAGAAGATG CAAAGGCGCC ACAAAAGAAG CACAAAGTTC TTTTGCAACA GTTCAACACA
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGTGGCT CTGATGAAGT TCTGTTTAAA
    GTCTATTGCG TGGACCACAC CTACACAACC ATCCGGGTAC CGGTGGCCGC CTCGGTGAAG
    GAAGTCCTCA GCGCCGTTGC CGACAAACTG GGCTCCGGGG AAGGCCTGAT CATCGTCAAG
    ATGAGTTCCG GGGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACAACG
    CTCACCATTA ATGGACGTCT GTTTGCTTGC CCGCGAGAGC AATTCGATTC ACTGACTCCC
    TTGCCAGAAC AGGAGGGCCC AACTGTTGGA ACAATGGGAA CTTTCGAACT GATGAGCTCC
    AAAGATTTGG CCTACCAGAT GACAATTTAT GATTGGGAGC TCTTCAACTG TGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATCATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGGAGGTT TAATGAAATT CAGTTTTGGG TTGTCACTGA GATCTGCCTT
    TGTTCCCAGC CTAGCAAGCG TGTTCAGCTC TTAAAAAAAT TTATTAAGAT CGCAGCCCAC
    TGTAAGGAGT ACAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAATGTA
    GCTGTGAGCC GCCTGGCACT GACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCAGAGTTTG AAAGTTTAAT GGATCCTTCA AGAAACCACA GGGCCTACAG GCTGACAGTA
    GCCAAATTGG ACCCTCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC GTTCATTGAC AATCTCGTAA ACTTTGAAAA AATGCGAATG
    ATTGCAAATA CTGCCAGAAC AGTGAGATAC TGCAGGAGCC AACCCTTCAA TCCTGATGCA
    GCTCAAGCTA ATAAGAACCA CCAGGATGTC CGGAGTTACG TTCGGCAATT AAATGTGATC
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGACTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800211.1
    CDS36..2612
    Translation

    Target ORF information:

    RefSeq Version XM_010801909.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801909.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACAC ACCTCTCATT GAACCTCACG TTCCTCTTCG TCCTGCTAAC
    ACCATTACCA AGGTTCCTTC AGAGAAGATC CTCAGGGCTG GAAAAATTTT ACGAAACGCC
    ATTCTCTCTC GAGCACCTCA CATGATAAGA GATAGAAAAT ACCACCTAAA GACATACAGA
    CAGTGCTGTG TGGGGACCGA GCTGGTGGAC TGGATGCTGC AGCAGACTCC ATGTGTTCAC
    TCCCGAACGC AAGCTGTGGG CATGTGGCAA GTCCTGGTAG AAGACAGCGT TCTCAACCAT
    GTGGATCAGG AGCATCACTT CCAAGACAAA TATTTATTTT ATCGGTTTCT GGATGATGAG
    CATGAGGACG CCCCTTTGCC TACGGAGGAG GAAAAAAAGG AGTGTGATGA GGAGCTGCAG
    GACACCATGC TGCTGCTGTC GCAGATGGGC CCGGACGCCC ACATGAGGAT GGTCCTTCGC
    AAACCACCTG GACAGAGGAC CATGGATGAC CTAGAAATTA TCTATGAAGA ACTCCTTCAC
    ATTAAAGCCT TATCCCATCT TTCTACCACA GTGAAACGGG AGTTGGCAGC GGTTCTCATT
    TTTGAGTCTC ATGCCAAAGG AGGGACTGTG TTATTTAACC AAGGGGAAGA AGGCACCTCC
    TGGTACATTA TTCTAAAAGG ATCAGTGAAT GTAGTCATTT ATGGCAAGGG TGTGGTCTGT
    ACCCTGCATG AGGGCGATGA CTTTGGCAAG TTAGCCCTAG TGAATGATGC CCCAAGAGCT
    GCCTCCATCG TCTTGAGAGA AGATAACTGC CATTTCTTAA GAGTAGACAA GGAGGATTTC
    AACCGGATCC TGAGGGACGT TGAGGCGAAT ACAGTCAGAC TTAAAGAACA TGACCAAGAT
    GTCTTGGTGC TGGAGAAGGT CCCAGCAGGG AACAGGGCTT CTAATCAAGG AAACTCACAG
    CCTCAGCAAA AGTATACTGT GATGTCAGGA ACACCTGAAA AAATTTTAGA GCATTTTCTA
    GAAACGATAC GCCTTGAGCC AGCTTTAAAT GAAGCAACAG ATTCTGTTTT AAATGACTTT
    GTTATGATGC ACTGTGTTTT TATGCCAAAT ACCCAGCTTT GCCCAGCTCT GGTGGCCCAT
    TACCATGCAC AGCCTTCGCA AGGTACAGAA CAAGAGAAAA TGGATTATGC CCTCAACAAC
    AAGAGGCGAG TCATCCGCCT GGTTTTGCAG TGGGCTGCCA TGTATGGAGA CATCCTACAA
    GAGGATGACG TGGCCATGGC TTTCCTGGAG GAGTTTTATG TGTCTGTATC AGATGATGCC
    CGGATGCTTG CCGCCCTCAA GGAGCAACTG CCTGAGCTGG AGAAGATTGT CAAGCAAATT
    TCAGAAGATG CAAAGGCGCC ACAAAAGAAG CACAAAGTTC TTTTGCAACA GTTCAACACA
    GGCGATGAGA GAGCCCAGAA GCGCCAGCCT ATCCGTGGCT CTGATGAAGT TCTGTTTAAA
    GTCTATTGCG TGGACCACAC CTACACAACC ATCCGGGTAC CGGTGGCCGC CTCGGTGAAG
    GAAGTCCTCA GCGCCGTTGC CGACAAACTG GGCTCCGGGG AAGGCCTGAT CATCGTCAAG
    ATGAGTTCCG GGGGAGAAAA GGTGGTGCTC AAACCTAATG ATGTTTCAGT ATTTACAACG
    CTCACCATTA ATGGACGTCT GTTTGCTTGC CCGCGAGAGC AATTCGATTC ACTGACTCCC
    TTGCCAGAAC AGGAGGGCCC AACTGTTGGA ACAATGGGAA CTTTCGAACT GATGAGCTCC
    AAAGATTTGG CCTACCAGAT GACAATTTAT GATTGGGAGC TCTTCAACTG TGTGCATGAG
    CTGGAGCTAA TCTATCACAC ATTTGGAAGG CATCATTTTA AAAAGACCAC AGCAAACTTG
    GATTTGTTCC TGAGGAGGTT TAATGAAATT CAGTTTTGGG TTGTCACTGA GATCTGCCTT
    TGTTCCCAGC CTAGCAAGCG TGTTCAGCTC TTAAAAAAAT TTATTAAGAT CGCAGCCCAC
    TGTAAGGAGT ACAAAAATCT GAATTCCTTT TTTGCCATCG TCATGGGACT AAGTAATGTA
    GCTGTGAGCC GCCTGGCACT GACGTGGGAG AAACTGCCAA GCAAGTTCAA GAAGTTCTAT
    GCAGAGTTTG AAAGTTTAAT GGATCCTTCA AGAAACCACA GGGCCTACAG GCTGACAGTA
    GCCAAATTGG ACCCTCCTCT CATCCCCTTC ATGCCTTTGC TCATTAAAGA TATGACATTT
    ACTCATGAGG GGAACAAGAC GTTCATTGAC AATCTCGTAA ACTTTGAAAA AATGCGAATG
    ATTGCAAATA CTGCCAGAAC AGTGAGATAC TGCAGGAGCC AACCCTTCAA TCCTGATGCA
    GCTCAAGCTA ATAAGAACCA CCAGGATGTC CGGAGTTACG TTCGGCAATT AAATGTGATC
    GACAACCAGA GAACTTTATC ACAGATGTCA CACAGACTAG AGCCTCGTCG ACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16344
    Clone ID Related Accession (Same CDS sequence) XM_005202345.3
    Accession Version XM_005202345.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2514bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202345.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCCCTTCC CCCAGACACC TCTCATTGAA CCTCACGTTC CTCTTCGTCC TGCTAACACC 
    ATTACCAAGG TTCCTTCAGA GAAGATCCTC AGGGCTGGAA AAATTTTACG AAACGCCATT
    CTCTCTCGAG CACCTCACAT GATAAGAGAT AGAAAATACC ACCTAAAGAC ATACAGACAG
    TGCTGTGTGG GGACCGAGCT GGTGGACTGG ATGCTGCAGC AGACTCCATG TGTTCACTCC
    CGAACGCAAG CTGTGGGCAT GTGGCAAGTC CTGGTAGAAG ACAGCGTTCT CAACCATGTG
    GATCAGGAGC ATCACTTCCA AGACAAATAT TTATTTTATC GGTTTCTGGA TGATGAGCAT
    GAGGACGCCC CTTTGCCTAC GGAGGAGGAA AAAAAGGAGT GTGATGAGGA GCTGCAGGAC
    ACCATGCTGC TGCTGTCGCA GATGGGCCCG GACGCCCACA TGAGGATGGT CCTTCGCAAA
    CCACCTGGAC AGAGGACCAT GGATGACCTA GAAATTATCT ATGAAGAACT CCTTCACATT
    AAAGCCTTAT CCCATCTTTC TACCACAGTG AAACGGGAGT TGGCAGCGGT TCTCATTTTT
    GAGTCTCATG CCAAAGGAGG GACTGTGTTA TTTAACCAAG GGGAAGAAGG CACCTCCTGG
    TACATTATTC TAAAAGGATC AGTGAATGTA GTCATTTATG GCAAGGGTGT GGTCTGTACC
    CTGCATGAGG GCGATGACTT TGGCAAGTTA GCCCTAGTGA ATGATGCCCC AAGAGCTGCC
    TCCATCGTCT TGAGAGAAGA TAACTGCCAT TTCTTAAGAG TAGACAAGGA GGATTTCAAC
    CGGATCCTGA GGGACGTTGA GGCGAATACA GTCAGACTTA AAGAACATGA CCAAGATGTC
    TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC AGGGCTTCTA ATCAAGGAAA CTCACAGCCT
    CAGCAAAAGT ATACTGTGAT GTCAGGAACA CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA
    ACGATACGCC TTGAGCCAGC TTTAAATGAA GCAACAGATT CTGTTTTAAA TGACTTTGTT
    ATGATGCACT GTGTTTTTAT GCCAAATACC CAGCTTTGCC CAGCTCTGGT GGCCCATTAC
    CATGCACAGC CTTCGCAAGG TACAGAACAA GAGAAAATGG ATTATGCCCT CAACAACAAG
    AGGCGAGTCA TCCGCCTGGT TTTGCAGTGG GCTGCCATGT ATGGAGACAT CCTACAAGAG
    GATGACGTGG CCATGGCTTT CCTGGAGGAG TTTTATGTGT CTGTATCAGA TGATGCCCGG
    ATGCTTGCCG CCCTCAAGGA GCAACTGCCT GAGCTGGAGA AGATTGTCAA GCAAATTTCA
    GAAGATGCAA AGGCGCCACA AAAGAAGCAC AAAGTTCTTT TGCAACAGTT CAACACAGGC
    GATGAGAGAG CCCAGAAGCG CCAGCCTATC CGTGGCTCTG ATGAAGTTCT GTTTAAAGTC
    TATTGCGTGG ACCACACCTA CACAACCATC CGGGTACCGG TGGCCGCCTC GGTGAAGGAA
    GTCCTCAGCG CCGTTGCCGA CAAACTGGGC TCCGGGGAAG GCCTGATCAT CGTCAAGATG
    AGTTCCGGGG GAGAAAAGGT GGTGCTCAAA CCTAATGATG TTTCAGTATT TACAACGCTC
    ACCATTAATG GACGTCTGTT TGCTTGCCCG CGAGAGCAAT TCGATTCACT GACTCCCTTG
    CCAGAACAGG AGGGCCCAAC TGTTGGAACA ATGGGAACTT TCGAACTGAT GAGCTCCAAA
    GATTTGGCCT ACCAGATGAC AATTTATGAT TGGGAGCTCT TCAACTGTGT GCATGAGCTG
    GAGCTAATCT ATCACACATT TGGAAGGCAT CATTTTAAAA AGACCACAGC AAACTTGGAT
    TTGTTCCTGA GGAGGTTTAA TGAAATTCAG TTTTGGGTTG TCACTGAGAT CTGCCTTTGT
    TCCCAGCCTA GCAAGCGTGT TCAGCTCTTA AAAAAATTTA TTAAGATCGC AGCCCACTGT
    AAGGAGTACA AAAATCTGAA TTCCTTTTTT GCCATCGTCA TGGGACTAAG TAATGTAGCT
    GTGAGCCGCC TGGCACTGAC GTGGGAGAAA CTGCCAAGCA AGTTCAAGAA GTTCTATGCA
    GAGTTTGAAA GTTTAATGGA TCCTTCAAGA AACCACAGGG CCTACAGGCT GACAGTAGCC
    AAATTGGACC CTCCTCTCAT CCCCTTCATG CCTTTGCTCA TTAAAGATAT GACATTTACT
    CATGAGGGGA ACAAGACGTT CATTGACAAT CTCGTAAACT TTGAAAAAAT GCGAATGATT
    GCAAATACTG CCAGAACAGT GAGATACTGC AGGAGCCAAC CCTTCAATCC TGATGCAGCT
    CAAGCTAATA AGAACCACCA GGATGTCCGG AGTTACGTTC GGCAATTAAA TGTGATCGAC
    AACCAGAGAA CTTTATCACA GATGTCACAC AGACTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202402.1
    CDS133..2646
    Translation

    Target ORF information:

    RefSeq Version XM_005202345.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202345.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCCCTTCC CCCAGACACC TCTCATTGAA CCTCACGTTC CTCTTCGTCC TGCTAACACC 
    ATTACCAAGG TTCCTTCAGA GAAGATCCTC AGGGCTGGAA AAATTTTACG AAACGCCATT
    CTCTCTCGAG CACCTCACAT GATAAGAGAT AGAAAATACC ACCTAAAGAC ATACAGACAG
    TGCTGTGTGG GGACCGAGCT GGTGGACTGG ATGCTGCAGC AGACTCCATG TGTTCACTCC
    CGAACGCAAG CTGTGGGCAT GTGGCAAGTC CTGGTAGAAG ACAGCGTTCT CAACCATGTG
    GATCAGGAGC ATCACTTCCA AGACAAATAT TTATTTTATC GGTTTCTGGA TGATGAGCAT
    GAGGACGCCC CTTTGCCTAC GGAGGAGGAA AAAAAGGAGT GTGATGAGGA GCTGCAGGAC
    ACCATGCTGC TGCTGTCGCA GATGGGCCCG GACGCCCACA TGAGGATGGT CCTTCGCAAA
    CCACCTGGAC AGAGGACCAT GGATGACCTA GAAATTATCT ATGAAGAACT CCTTCACATT
    AAAGCCTTAT CCCATCTTTC TACCACAGTG AAACGGGAGT TGGCAGCGGT TCTCATTTTT
    GAGTCTCATG CCAAAGGAGG GACTGTGTTA TTTAACCAAG GGGAAGAAGG CACCTCCTGG
    TACATTATTC TAAAAGGATC AGTGAATGTA GTCATTTATG GCAAGGGTGT GGTCTGTACC
    CTGCATGAGG GCGATGACTT TGGCAAGTTA GCCCTAGTGA ATGATGCCCC AAGAGCTGCC
    TCCATCGTCT TGAGAGAAGA TAACTGCCAT TTCTTAAGAG TAGACAAGGA GGATTTCAAC
    CGGATCCTGA GGGACGTTGA GGCGAATACA GTCAGACTTA AAGAACATGA CCAAGATGTC
    TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC AGGGCTTCTA ATCAAGGAAA CTCACAGCCT
    CAGCAAAAGT ATACTGTGAT GTCAGGAACA CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA
    ACGATACGCC TTGAGCCAGC TTTAAATGAA GCAACAGATT CTGTTTTAAA TGACTTTGTT
    ATGATGCACT GTGTTTTTAT GCCAAATACC CAGCTTTGCC CAGCTCTGGT GGCCCATTAC
    CATGCACAGC CTTCGCAAGG TACAGAACAA GAGAAAATGG ATTATGCCCT CAACAACAAG
    AGGCGAGTCA TCCGCCTGGT TTTGCAGTGG GCTGCCATGT ATGGAGACAT CCTACAAGAG
    GATGACGTGG CCATGGCTTT CCTGGAGGAG TTTTATGTGT CTGTATCAGA TGATGCCCGG
    ATGCTTGCCG CCCTCAAGGA GCAACTGCCT GAGCTGGAGA AGATTGTCAA GCAAATTTCA
    GAAGATGCAA AGGCGCCACA AAAGAAGCAC AAAGTTCTTT TGCAACAGTT CAACACAGGC
    GATGAGAGAG CCCAGAAGCG CCAGCCTATC CGTGGCTCTG ATGAAGTTCT GTTTAAAGTC
    TATTGCGTGG ACCACACCTA CACAACCATC CGGGTACCGG TGGCCGCCTC GGTGAAGGAA
    GTCCTCAGCG CCGTTGCCGA CAAACTGGGC TCCGGGGAAG GCCTGATCAT CGTCAAGATG
    AGTTCCGGGG GAGAAAAGGT GGTGCTCAAA CCTAATGATG TTTCAGTATT TACAACGCTC
    ACCATTAATG GACGTCTGTT TGCTTGCCCG CGAGAGCAAT TCGATTCACT GACTCCCTTG
    CCAGAACAGG AGGGCCCAAC TGTTGGAACA ATGGGAACTT TCGAACTGAT GAGCTCCAAA
    GATTTGGCCT ACCAGATGAC AATTTATGAT TGGGAGCTCT TCAACTGTGT GCATGAGCTG
    GAGCTAATCT ATCACACATT TGGAAGGCAT CATTTTAAAA AGACCACAGC AAACTTGGAT
    TTGTTCCTGA GGAGGTTTAA TGAAATTCAG TTTTGGGTTG TCACTGAGAT CTGCCTTTGT
    TCCCAGCCTA GCAAGCGTGT TCAGCTCTTA AAAAAATTTA TTAAGATCGC AGCCCACTGT
    AAGGAGTACA AAAATCTGAA TTCCTTTTTT GCCATCGTCA TGGGACTAAG TAATGTAGCT
    GTGAGCCGCC TGGCACTGAC GTGGGAGAAA CTGCCAAGCA AGTTCAAGAA GTTCTATGCA
    GAGTTTGAAA GTTTAATGGA TCCTTCAAGA AACCACAGGG CCTACAGGCT GACAGTAGCC
    AAATTGGACC CTCCTCTCAT CCCCTTCATG CCTTTGCTCA TTAAAGATAT GACATTTACT
    CATGAGGGGA ACAAGACGTT CATTGACAAT CTCGTAAACT TTGAAAAAAT GCGAATGATT
    GCAAATACTG CCAGAACAGT GAGATACTGC AGGAGCCAAC CCTTCAATCC TGATGCAGCT
    CAAGCTAATA AGAACCACCA GGATGTCCGG AGTTACGTTC GGCAATTAAA TGTGATCGAC
    AACCAGAGAA CTTTATCACA GATGTCACAC AGACTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53626
    Clone ID Related Accession (Same CDS sequence) XM_010801907.3 , XM_010801907.2
    Accession Version XM_010801907.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801907.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCCTGCA AGAAATATCG ACAGTACATG GCAGGACTTC TGGCTCCTCC TTATGGTGTT 
    ATGGAAACGG GCTCTAACAA TGACAGGATT CCTGACAAGG AGAACACACC TCTCATTGAA
    CCTCACGTTC CTCTTCGTCC TGCTAACACC ATTACCAAGG TTCCTTCAGA GAAGATCCTC
    AGGGCTGGAA AAATTTTACG AAACGCCATT CTCTCTCGAG CACCTCACAT GATAAGAGAT
    AGAAAATACC ACCTAAAGAC ATACAGACAG TGCTGTGTGG GGACCGAGCT GGTGGACTGG
    ATGCTGCAGC AGACTCCATG TGTTCACTCC CGAACGCAAG CTGTGGGCAT GTGGCAAGTC
    CTGGTAGAAG ACAGCGTTCT CAACCATGTG GATCAGGAGC ATCACTTCCA AGACAAATAT
    TTATTTTATC GGTTTCTGGA TGATGAGCAT GAGGACGCCC CTTTGCCTAC GGAGGAGGAA
    AAAAAGGAGT GTGATGAGGA GCTGCAGGAC ACCATGCTGC TGCTGTCGCA GATGGGCCCG
    GACGCCCACA TGAGGATGGT CCTTCGCAAA CCACCTGGAC AGAGGACCAT GGATGACCTA
    GAAATTATCT ATGAAGAACT CCTTCACATT AAAGCCTTAT CCCATCTTTC TACCACAGTG
    AAACGGGAGT TGGCAGCGGT TCTCATTTTT GAGTCTCATG CCAAAGGAGG GACTGTGTTA
    TTTAACCAAG GGGAAGAAGG CACCTCCTGG TACATTATTC TAAAAGGATC AGTGAATGTA
    GTCATTTATG GCAAGGGTGT GGTCTGTACC CTGCATGAGG GCGATGACTT TGGCAAGTTA
    GCCCTAGTGA ATGATGCCCC AAGAGCTGCC TCCATCGTCT TGAGAGAAGA TAACTGCCAT
    TTCTTAAGAG TAGACAAGGA GGATTTCAAC CGGATCCTGA GGGACGTTGA GGCGAATACA
    GTCAGACTTA AAGAACATGA CCAAGATGTC TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC
    AGGGCTTCTA ATCAAGGAAA CTCACAGCCT CAGCAAAAGT ATACTGTGAT GTCAGGAACA
    CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA ACGATACGCC TTGAGCCAGC TTTAAATGAA
    GCAACAGATT CTGTTTTAAA TGACTTTGTT ATGATGCACT GTGTTTTTAT GCCAAATACC
    CAGCTTTGCC CAGCTCTGGT GGCCCATTAC CATGCACAGC CTTCGCAAGG TACAGAACAA
    GAGAAAATGG ATTATGCCCT CAACAACAAG AGGCGAGTCA TCCGCCTGGT TTTGCAGTGG
    GCTGCCATGT ATGGAGACAT CCTACAAGAG GATGACGTGG CCATGGCTTT CCTGGAGGAG
    TTTTATGTGT CTGTATCAGA TGATGCCCGG ATGCTTGCCG CCCTCAAGGA GCAACTGCCT
    GAGCTGGAGA AGATTGTCAA GCAAATTTCA GAAGATGCAA AGGCGCCACA AAAGAAGCAC
    AAAGTTCTTT TGCAACAGTT CAACACAGGC GATGAGAGAG CCCAGAAGCG CCAGCCTATC
    CGTGGCTCTG ATGAAGTTCT GTTTAAAGTC TATTGCGTGG ACCACACCTA CACAACCATC
    CGGGTACCGG TGGCCGCCTC GGTGAAGGAA GTCCTCAGCG CCGTTGCCGA CAAACTGGGC
    TCCGGGGAAG GCCTGATCAT CGTCAAGATG AGTTCCGGGG GAGAAAAGGT GGTGCTCAAA
    CCTAATGATG TTTCAGTATT TACAACGCTC ACCATTAATG GACGTCTGTT TGCTTGCCCG
    CGAGAGCAAT TCGATTCACT GACTCCCTTG CCAGAACAGG AGGGCCCAAC TGTTGGAACA
    ATGGGAACTT TCGAACTGAT GAGCTCCAAA GATTTGGCCT ACCAGATGAC AATTTATGAT
    TGGGAGCTCT TCAACTGTGT GCATGAGCTG GAGCTAATCT ATCACACATT TGGAAGGCAT
    CATTTTAAAA AGACCACAGC AAACTTGGAT TTGTTCCTGA GGAGGTTTAA TGAAATTCAG
    TTTTGGGTTG TCACTGAGAT CTGCCTTTGT TCCCAGCCTA GCAAGCGTGT TCAGCTCTTA
    AAAAAATTTA TTAAGATCGC AGCCCACTGT AAGGAGTACA AAAATCTGAA TTCCTTTTTT
    GCCATCGTCA TGGGACTAAG TAATGTAGCT GTGAGCCGCC TGGCACTGAC GTGGGAGAAA
    CTGCCAAGCA AGTTCAAGAA GTTCTATGCA GAGTTTGAAA GTTTAATGGA TCCTTCAAGA
    AACCACAGGG CCTACAGGCT GACAGTAGCC AAATTGGACC CTCCTCTCAT CCCCTTCATG
    CCTTTGCTCA TTAAAGATAT GACATTTACT CATGAGGGGA ACAAGACGTT CATTGACAAT
    CTCGTAAACT TTGAAAAAAT GCGAATGATT GCAAATACTG CCAGAACAGT GAGATACTGC
    AGGAGCCAAC CCTTCAATCC TGATGCAGCT CAAGCTAATA AGAACCACCA GGATGTCCGG
    AGTTACGTTC GGCAATTAAA TGTGATCGAC AACCAGAGAA CTTTATCACA GATGTCACAC
    AGACTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800209.1
    CDS362..2965
    Translation

    Target ORF information:

    RefSeq Version XM_010801907.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801907.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCCTGCA AGAAATATCG ACAGTACATG GCAGGACTTC TGGCTCCTCC TTATGGTGTT 
    ATGGAAACGG GCTCTAACAA TGACAGGATT CCTGACAAGG AGAACACACC TCTCATTGAA
    CCTCACGTTC CTCTTCGTCC TGCTAACACC ATTACCAAGG TTCCTTCAGA GAAGATCCTC
    AGGGCTGGAA AAATTTTACG AAACGCCATT CTCTCTCGAG CACCTCACAT GATAAGAGAT
    AGAAAATACC ACCTAAAGAC ATACAGACAG TGCTGTGTGG GGACCGAGCT GGTGGACTGG
    ATGCTGCAGC AGACTCCATG TGTTCACTCC CGAACGCAAG CTGTGGGCAT GTGGCAAGTC
    CTGGTAGAAG ACAGCGTTCT CAACCATGTG GATCAGGAGC ATCACTTCCA AGACAAATAT
    TTATTTTATC GGTTTCTGGA TGATGAGCAT GAGGACGCCC CTTTGCCTAC GGAGGAGGAA
    AAAAAGGAGT GTGATGAGGA GCTGCAGGAC ACCATGCTGC TGCTGTCGCA GATGGGCCCG
    GACGCCCACA TGAGGATGGT CCTTCGCAAA CCACCTGGAC AGAGGACCAT GGATGACCTA
    GAAATTATCT ATGAAGAACT CCTTCACATT AAAGCCTTAT CCCATCTTTC TACCACAGTG
    AAACGGGAGT TGGCAGCGGT TCTCATTTTT GAGTCTCATG CCAAAGGAGG GACTGTGTTA
    TTTAACCAAG GGGAAGAAGG CACCTCCTGG TACATTATTC TAAAAGGATC AGTGAATGTA
    GTCATTTATG GCAAGGGTGT GGTCTGTACC CTGCATGAGG GCGATGACTT TGGCAAGTTA
    GCCCTAGTGA ATGATGCCCC AAGAGCTGCC TCCATCGTCT TGAGAGAAGA TAACTGCCAT
    TTCTTAAGAG TAGACAAGGA GGATTTCAAC CGGATCCTGA GGGACGTTGA GGCGAATACA
    GTCAGACTTA AAGAACATGA CCAAGATGTC TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC
    AGGGCTTCTA ATCAAGGAAA CTCACAGCCT CAGCAAAAGT ATACTGTGAT GTCAGGAACA
    CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA ACGATACGCC TTGAGCCAGC TTTAAATGAA
    GCAACAGATT CTGTTTTAAA TGACTTTGTT ATGATGCACT GTGTTTTTAT GCCAAATACC
    CAGCTTTGCC CAGCTCTGGT GGCCCATTAC CATGCACAGC CTTCGCAAGG TACAGAACAA
    GAGAAAATGG ATTATGCCCT CAACAACAAG AGGCGAGTCA TCCGCCTGGT TTTGCAGTGG
    GCTGCCATGT ATGGAGACAT CCTACAAGAG GATGACGTGG CCATGGCTTT CCTGGAGGAG
    TTTTATGTGT CTGTATCAGA TGATGCCCGG ATGCTTGCCG CCCTCAAGGA GCAACTGCCT
    GAGCTGGAGA AGATTGTCAA GCAAATTTCA GAAGATGCAA AGGCGCCACA AAAGAAGCAC
    AAAGTTCTTT TGCAACAGTT CAACACAGGC GATGAGAGAG CCCAGAAGCG CCAGCCTATC
    CGTGGCTCTG ATGAAGTTCT GTTTAAAGTC TATTGCGTGG ACCACACCTA CACAACCATC
    CGGGTACCGG TGGCCGCCTC GGTGAAGGAA GTCCTCAGCG CCGTTGCCGA CAAACTGGGC
    TCCGGGGAAG GCCTGATCAT CGTCAAGATG AGTTCCGGGG GAGAAAAGGT GGTGCTCAAA
    CCTAATGATG TTTCAGTATT TACAACGCTC ACCATTAATG GACGTCTGTT TGCTTGCCCG
    CGAGAGCAAT TCGATTCACT GACTCCCTTG CCAGAACAGG AGGGCCCAAC TGTTGGAACA
    ATGGGAACTT TCGAACTGAT GAGCTCCAAA GATTTGGCCT ACCAGATGAC AATTTATGAT
    TGGGAGCTCT TCAACTGTGT GCATGAGCTG GAGCTAATCT ATCACACATT TGGAAGGCAT
    CATTTTAAAA AGACCACAGC AAACTTGGAT TTGTTCCTGA GGAGGTTTAA TGAAATTCAG
    TTTTGGGTTG TCACTGAGAT CTGCCTTTGT TCCCAGCCTA GCAAGCGTGT TCAGCTCTTA
    AAAAAATTTA TTAAGATCGC AGCCCACTGT AAGGAGTACA AAAATCTGAA TTCCTTTTTT
    GCCATCGTCA TGGGACTAAG TAATGTAGCT GTGAGCCGCC TGGCACTGAC GTGGGAGAAA
    CTGCCAAGCA AGTTCAAGAA GTTCTATGCA GAGTTTGAAA GTTTAATGGA TCCTTCAAGA
    AACCACAGGG CCTACAGGCT GACAGTAGCC AAATTGGACC CTCCTCTCAT CCCCTTCATG
    CCTTTGCTCA TTAAAGATAT GACATTTACT CATGAGGGGA ACAAGACGTT CATTGACAAT
    CTCGTAAACT TTGAAAAAAT GCGAATGATT GCAAATACTG CCAGAACAGT GAGATACTGC
    AGGAGCCAAC CCTTCAATCC TGATGCAGCT CAAGCTAATA AGAACCACCA GGATGTCCGG
    AGTTACGTTC GGCAATTAAA TGTGATCGAC AACCAGAGAA CTTTATCACA GATGTCACAC
    AGACTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53626
    Clone ID Related Accession (Same CDS sequence) XM_010801907.3 , XM_010801907.2
    Accession Version XM_010801907.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801907.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCCTGCA AGAAATATCG ACAGTACATG GCAGGACTTC TGGCTCCTCC TTATGGTGTT 
    ATGGAAACGG GCTCTAACAA TGACAGGATT CCTGACAAGG AGAACACACC TCTCATTGAA
    CCTCACGTTC CTCTTCGTCC TGCTAACACC ATTACCAAGG TTCCTTCAGA GAAGATCCTC
    AGGGCTGGAA AAATTTTACG AAACGCCATT CTCTCTCGAG CACCTCACAT GATAAGAGAT
    AGAAAATACC ACCTAAAGAC ATACAGACAG TGCTGTGTGG GGACCGAGCT GGTGGACTGG
    ATGCTGCAGC AGACTCCATG TGTTCACTCC CGAACGCAAG CTGTGGGCAT GTGGCAAGTC
    CTGGTAGAAG ACAGCGTTCT CAACCATGTG GATCAGGAGC ATCACTTCCA AGACAAATAT
    TTATTTTATC GGTTTCTGGA TGATGAGCAT GAGGACGCCC CTTTGCCTAC GGAGGAGGAA
    AAAAAGGAGT GTGATGAGGA GCTGCAGGAC ACCATGCTGC TGCTGTCGCA GATGGGCCCG
    GACGCCCACA TGAGGATGGT CCTTCGCAAA CCACCTGGAC AGAGGACCAT GGATGACCTA
    GAAATTATCT ATGAAGAACT CCTTCACATT AAAGCCTTAT CCCATCTTTC TACCACAGTG
    AAACGGGAGT TGGCAGCGGT TCTCATTTTT GAGTCTCATG CCAAAGGAGG GACTGTGTTA
    TTTAACCAAG GGGAAGAAGG CACCTCCTGG TACATTATTC TAAAAGGATC AGTGAATGTA
    GTCATTTATG GCAAGGGTGT GGTCTGTACC CTGCATGAGG GCGATGACTT TGGCAAGTTA
    GCCCTAGTGA ATGATGCCCC AAGAGCTGCC TCCATCGTCT TGAGAGAAGA TAACTGCCAT
    TTCTTAAGAG TAGACAAGGA GGATTTCAAC CGGATCCTGA GGGACGTTGA GGCGAATACA
    GTCAGACTTA AAGAACATGA CCAAGATGTC TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC
    AGGGCTTCTA ATCAAGGAAA CTCACAGCCT CAGCAAAAGT ATACTGTGAT GTCAGGAACA
    CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA ACGATACGCC TTGAGCCAGC TTTAAATGAA
    GCAACAGATT CTGTTTTAAA TGACTTTGTT ATGATGCACT GTGTTTTTAT GCCAAATACC
    CAGCTTTGCC CAGCTCTGGT GGCCCATTAC CATGCACAGC CTTCGCAAGG TACAGAACAA
    GAGAAAATGG ATTATGCCCT CAACAACAAG AGGCGAGTCA TCCGCCTGGT TTTGCAGTGG
    GCTGCCATGT ATGGAGACAT CCTACAAGAG GATGACGTGG CCATGGCTTT CCTGGAGGAG
    TTTTATGTGT CTGTATCAGA TGATGCCCGG ATGCTTGCCG CCCTCAAGGA GCAACTGCCT
    GAGCTGGAGA AGATTGTCAA GCAAATTTCA GAAGATGCAA AGGCGCCACA AAAGAAGCAC
    AAAGTTCTTT TGCAACAGTT CAACACAGGC GATGAGAGAG CCCAGAAGCG CCAGCCTATC
    CGTGGCTCTG ATGAAGTTCT GTTTAAAGTC TATTGCGTGG ACCACACCTA CACAACCATC
    CGGGTACCGG TGGCCGCCTC GGTGAAGGAA GTCCTCAGCG CCGTTGCCGA CAAACTGGGC
    TCCGGGGAAG GCCTGATCAT CGTCAAGATG AGTTCCGGGG GAGAAAAGGT GGTGCTCAAA
    CCTAATGATG TTTCAGTATT TACAACGCTC ACCATTAATG GACGTCTGTT TGCTTGCCCG
    CGAGAGCAAT TCGATTCACT GACTCCCTTG CCAGAACAGG AGGGCCCAAC TGTTGGAACA
    ATGGGAACTT TCGAACTGAT GAGCTCCAAA GATTTGGCCT ACCAGATGAC AATTTATGAT
    TGGGAGCTCT TCAACTGTGT GCATGAGCTG GAGCTAATCT ATCACACATT TGGAAGGCAT
    CATTTTAAAA AGACCACAGC AAACTTGGAT TTGTTCCTGA GGAGGTTTAA TGAAATTCAG
    TTTTGGGTTG TCACTGAGAT CTGCCTTTGT TCCCAGCCTA GCAAGCGTGT TCAGCTCTTA
    AAAAAATTTA TTAAGATCGC AGCCCACTGT AAGGAGTACA AAAATCTGAA TTCCTTTTTT
    GCCATCGTCA TGGGACTAAG TAATGTAGCT GTGAGCCGCC TGGCACTGAC GTGGGAGAAA
    CTGCCAAGCA AGTTCAAGAA GTTCTATGCA GAGTTTGAAA GTTTAATGGA TCCTTCAAGA
    AACCACAGGG CCTACAGGCT GACAGTAGCC AAATTGGACC CTCCTCTCAT CCCCTTCATG
    CCTTTGCTCA TTAAAGATAT GACATTTACT CATGAGGGGA ACAAGACGTT CATTGACAAT
    CTCGTAAACT TTGAAAAAAT GCGAATGATT GCAAATACTG CCAGAACAGT GAGATACTGC
    AGGAGCCAAC CCTTCAATCC TGATGCAGCT CAAGCTAATA AGAACCACCA GGATGTCCGG
    AGTTACGTTC GGCAATTAAA TGTGATCGAC AACCAGAGAA CTTTATCACA GATGTCACAC
    AGACTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800209.1
    CDS358..2961
    Translation

    Target ORF information:

    RefSeq Version XM_010801907.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801907.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCCTGCA AGAAATATCG ACAGTACATG GCAGGACTTC TGGCTCCTCC TTATGGTGTT 
    ATGGAAACGG GCTCTAACAA TGACAGGATT CCTGACAAGG AGAACACACC TCTCATTGAA
    CCTCACGTTC CTCTTCGTCC TGCTAACACC ATTACCAAGG TTCCTTCAGA GAAGATCCTC
    AGGGCTGGAA AAATTTTACG AAACGCCATT CTCTCTCGAG CACCTCACAT GATAAGAGAT
    AGAAAATACC ACCTAAAGAC ATACAGACAG TGCTGTGTGG GGACCGAGCT GGTGGACTGG
    ATGCTGCAGC AGACTCCATG TGTTCACTCC CGAACGCAAG CTGTGGGCAT GTGGCAAGTC
    CTGGTAGAAG ACAGCGTTCT CAACCATGTG GATCAGGAGC ATCACTTCCA AGACAAATAT
    TTATTTTATC GGTTTCTGGA TGATGAGCAT GAGGACGCCC CTTTGCCTAC GGAGGAGGAA
    AAAAAGGAGT GTGATGAGGA GCTGCAGGAC ACCATGCTGC TGCTGTCGCA GATGGGCCCG
    GACGCCCACA TGAGGATGGT CCTTCGCAAA CCACCTGGAC AGAGGACCAT GGATGACCTA
    GAAATTATCT ATGAAGAACT CCTTCACATT AAAGCCTTAT CCCATCTTTC TACCACAGTG
    AAACGGGAGT TGGCAGCGGT TCTCATTTTT GAGTCTCATG CCAAAGGAGG GACTGTGTTA
    TTTAACCAAG GGGAAGAAGG CACCTCCTGG TACATTATTC TAAAAGGATC AGTGAATGTA
    GTCATTTATG GCAAGGGTGT GGTCTGTACC CTGCATGAGG GCGATGACTT TGGCAAGTTA
    GCCCTAGTGA ATGATGCCCC AAGAGCTGCC TCCATCGTCT TGAGAGAAGA TAACTGCCAT
    TTCTTAAGAG TAGACAAGGA GGATTTCAAC CGGATCCTGA GGGACGTTGA GGCGAATACA
    GTCAGACTTA AAGAACATGA CCAAGATGTC TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC
    AGGGCTTCTA ATCAAGGAAA CTCACAGCCT CAGCAAAAGT ATACTGTGAT GTCAGGAACA
    CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA ACGATACGCC TTGAGCCAGC TTTAAATGAA
    GCAACAGATT CTGTTTTAAA TGACTTTGTT ATGATGCACT GTGTTTTTAT GCCAAATACC
    CAGCTTTGCC CAGCTCTGGT GGCCCATTAC CATGCACAGC CTTCGCAAGG TACAGAACAA
    GAGAAAATGG ATTATGCCCT CAACAACAAG AGGCGAGTCA TCCGCCTGGT TTTGCAGTGG
    GCTGCCATGT ATGGAGACAT CCTACAAGAG GATGACGTGG CCATGGCTTT CCTGGAGGAG
    TTTTATGTGT CTGTATCAGA TGATGCCCGG ATGCTTGCCG CCCTCAAGGA GCAACTGCCT
    GAGCTGGAGA AGATTGTCAA GCAAATTTCA GAAGATGCAA AGGCGCCACA AAAGAAGCAC
    AAAGTTCTTT TGCAACAGTT CAACACAGGC GATGAGAGAG CCCAGAAGCG CCAGCCTATC
    CGTGGCTCTG ATGAAGTTCT GTTTAAAGTC TATTGCGTGG ACCACACCTA CACAACCATC
    CGGGTACCGG TGGCCGCCTC GGTGAAGGAA GTCCTCAGCG CCGTTGCCGA CAAACTGGGC
    TCCGGGGAAG GCCTGATCAT CGTCAAGATG AGTTCCGGGG GAGAAAAGGT GGTGCTCAAA
    CCTAATGATG TTTCAGTATT TACAACGCTC ACCATTAATG GACGTCTGTT TGCTTGCCCG
    CGAGAGCAAT TCGATTCACT GACTCCCTTG CCAGAACAGG AGGGCCCAAC TGTTGGAACA
    ATGGGAACTT TCGAACTGAT GAGCTCCAAA GATTTGGCCT ACCAGATGAC AATTTATGAT
    TGGGAGCTCT TCAACTGTGT GCATGAGCTG GAGCTAATCT ATCACACATT TGGAAGGCAT
    CATTTTAAAA AGACCACAGC AAACTTGGAT TTGTTCCTGA GGAGGTTTAA TGAAATTCAG
    TTTTGGGTTG TCACTGAGAT CTGCCTTTGT TCCCAGCCTA GCAAGCGTGT TCAGCTCTTA
    AAAAAATTTA TTAAGATCGC AGCCCACTGT AAGGAGTACA AAAATCTGAA TTCCTTTTTT
    GCCATCGTCA TGGGACTAAG TAATGTAGCT GTGAGCCGCC TGGCACTGAC GTGGGAGAAA
    CTGCCAAGCA AGTTCAAGAA GTTCTATGCA GAGTTTGAAA GTTTAATGGA TCCTTCAAGA
    AACCACAGGG CCTACAGGCT GACAGTAGCC AAATTGGACC CTCCTCTCAT CCCCTTCATG
    CCTTTGCTCA TTAAAGATAT GACATTTACT CATGAGGGGA ACAAGACGTT CATTGACAAT
    CTCGTAAACT TTGAAAAAAT GCGAATGATT GCAAATACTG CCAGAACAGT GAGATACTGC
    AGGAGCCAAC CCTTCAATCC TGATGCAGCT CAAGCTAATA AGAACCACCA GGATGTCCGG
    AGTTACGTTC GGCAATTAAA TGTGATCGAC AACCAGAGAA CTTTATCACA GATGTCACAC
    AGACTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53625
    Clone ID Related Accession (Same CDS sequence) XM_015474949.2
    Accession Version XM_015474949.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474949.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCGGCCG AGTGGATCGC CTGTCTGGAT 
    AAAAGACCAC TGGAACGATC CAGTGAAGAT GTGGATATAA TCTTCACACG GCTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGTTT ATGTGGTTAT
    TATGAGAATC TGGAAAAAGG AATAACATTA TTTCGCCAAG GTGATATTGG AACCAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTTTCTG AGACCAGCAG TCACCAGGAC
    GCAGTGACCA TCTGCACTCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACCCCACGCC ATGCGACCAT CGTTACCAGG GAGAGCAGTG AGCTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTACA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTT
    CCTTCAGAGA AGATCCTCAG GGCTGGAAAA ATTTTACGAA ACGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAGTG CTGTGTGGGG
    ACCGAGCTGG TGGACTGGAT GCTGCAGCAG ACTCCATGTG TTCACTCCCG AACGCAAGCT
    GTGGGCATGT GGCAAGTCCT GGTAGAAGAC AGCGTTCTCA ACCATGTGGA TCAGGAGCAT
    CACTTCCAAG ACAAATATTT ATTTTATCGG TTTCTGGATG ATGAGCATGA GGACGCCCCT
    TTGCCTACGG AGGAGGAAAA AAAGGAGTGT GATGAGGAGC TGCAGGACAC CATGCTGCTG
    CTGTCGCAGA TGGGCCCGGA CGCCCACATG AGGATGGTCC TTCGCAAACC ACCTGGACAG
    AGGACCATGG ATGACCTAGA AATTATCTAT GAAGAACTCC TTCACATTAA AGCCTTATCC
    CATCTTTCTA CCACAGTGAA ACGGGAGTTG GCAGCGGTTC TCATTTTTGA GTCTCATGCC
    AAAGGAGGGA CTGTGTTATT TAACCAAGGG GAAGAAGGCA CCTCCTGGTA CATTATTCTA
    AAAGGATCAG TGAATGTAGT CATTTATGGC AAGGGTGTGG TCTGTACCCT GCATGAGGGC
    GATGACTTTG GCAAGTTAGC CCTAGTGAAT GATGCCCCAA GAGCTGCCTC CATCGTCTTG
    AGAGAAGATA ACTGCCATTT CTTAAGAGTA GACAAGGAGG ATTTCAACCG GATCCTGAGG
    GACGTTGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG GGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC GATACGCCTT
    GAGCCAGCTT TAAATGAAGC AACAGATTCT GTTTTAAATG ACTTTGTTAT GATGCACTGT
    GTTTTTATGC CAAATACCCA GCTTTGCCCA GCTCTGGTGG CCCATTACCA TGCACAGCCT
    TCGCAAGGTA CAGAACAAGA GAAAATGGAT TATGCCCTCA ACAACAAGAG GCGAGTCATC
    CGCCTGGTTT TGCAGTGGGC TGCCATGTAT GGAGACATCC TACAAGAGGA TGACGTGGCC
    ATGGCTTTCC TGGAGGAGTT TTATGTGTCT GTATCAGATG ATGCCCGGAT GCTTGCCGCC
    CTCAAGGAGC AACTGCCTGA GCTGGAGAAG ATTGTCAAGC AAATTTCAGA AGATGCAAAG
    GCGCCACAAA AGAAGCACAA AGTTCTTTTG CAACAGTTCA ACACAGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG TGGCTCTGAT GAAGTTCTGT TTAAAGTCTA TTGCGTGGAC
    CACACCTACA CAACCATCCG GGTACCGGTG GCCGCCTCGG TGAAGGAAGT CCTCAGCGCC
    GTTGCCGACA AACTGGGCTC CGGGGAAGGC CTGATCATCG TCAAGATGAG TTCCGGGGGA
    GAAAAGGTGG TGCTCAAACC TAATGATGTT TCAGTATTTA CAACGCTCAC CATTAATGGA
    CGTCTGTTTG CTTGCCCGCG AGAGCAATTC GATTCACTGA CTCCCTTGCC AGAACAGGAG
    GGCCCAACTG TTGGAACAAT GGGAACTTTC GAACTGATGA GCTCCAAAGA TTTGGCCTAC
    CAGATGACAA TTTATGATTG GGAGCTCTTC AACTGTGTGC ATGAGCTGGA GCTAATCTAT
    CACACATTTG GAAGGCATCA TTTTAAAAAG ACCACAGCAA ACTTGGATTT GTTCCTGAGG
    AGGTTTAATG AAATTCAGTT TTGGGTTGTC ACTGAGATCT GCCTTTGTTC CCAGCCTAGC
    AAGCGTGTTC AGCTCTTAAA AAAATTTATT AAGATCGCAG CCCACTGTAA GGAGTACAAA
    AATCTGAATT CCTTTTTTGC CATCGTCATG GGACTAAGTA ATGTAGCTGT GAGCCGCCTG
    GCACTGACGT GGGAGAAACT GCCAAGCAAG TTCAAGAAGT TCTATGCAGA GTTTGAAAGT
    TTAATGGATC CTTCAAGAAA CCACAGGGCC TACAGGCTGA CAGTAGCCAA ATTGGACCCT
    CCTCTCATCC CCTTCATGCC TTTGCTCATT AAAGATATGA CATTTACTCA TGAGGGGAAC
    AAGACGTTCA TTGACAATCT CGTAAACTTT GAAAAAATGC GAATGATTGC AAATACTGCC
    AGAACAGTGA GATACTGCAG GAGCCAACCC TTCAATCCTG ATGCAGCTCA AGCTAATAAG
    AACCACCAGG ATGTCCGGAG TTACGTTCGG CAATTAAATG TGATCGACAA CCAGAGAACT
    TTATCACAGA TGTCACACAG ACTAGAGCCT CGTCGACCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330435.2
    CDS99..3080
    Translation

    Target ORF information:

    RefSeq Version XM_015474949.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474949.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCGGCCG AGTGGATCGC CTGTCTGGAT 
    AAAAGACCAC TGGAACGATC CAGTGAAGAT GTGGATATAA TCTTCACACG GCTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGTTT ATGTGGTTAT
    TATGAGAATC TGGAAAAAGG AATAACATTA TTTCGCCAAG GTGATATTGG AACCAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTTTCTG AGACCAGCAG TCACCAGGAC
    GCAGTGACCA TCTGCACTCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACCCCACGCC ATGCGACCAT CGTTACCAGG GAGAGCAGTG AGCTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTACA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACGTT
    CCTTCAGAGA AGATCCTCAG GGCTGGAAAA ATTTTACGAA ACGCCATTCT CTCTCGAGCA
    CCTCACATGA TAAGAGATAG AAAATACCAC CTAAAGACAT ACAGACAGTG CTGTGTGGGG
    ACCGAGCTGG TGGACTGGAT GCTGCAGCAG ACTCCATGTG TTCACTCCCG AACGCAAGCT
    GTGGGCATGT GGCAAGTCCT GGTAGAAGAC AGCGTTCTCA ACCATGTGGA TCAGGAGCAT
    CACTTCCAAG ACAAATATTT ATTTTATCGG TTTCTGGATG ATGAGCATGA GGACGCCCCT
    TTGCCTACGG AGGAGGAAAA AAAGGAGTGT GATGAGGAGC TGCAGGACAC CATGCTGCTG
    CTGTCGCAGA TGGGCCCGGA CGCCCACATG AGGATGGTCC TTCGCAAACC ACCTGGACAG
    AGGACCATGG ATGACCTAGA AATTATCTAT GAAGAACTCC TTCACATTAA AGCCTTATCC
    CATCTTTCTA CCACAGTGAA ACGGGAGTTG GCAGCGGTTC TCATTTTTGA GTCTCATGCC
    AAAGGAGGGA CTGTGTTATT TAACCAAGGG GAAGAAGGCA CCTCCTGGTA CATTATTCTA
    AAAGGATCAG TGAATGTAGT CATTTATGGC AAGGGTGTGG TCTGTACCCT GCATGAGGGC
    GATGACTTTG GCAAGTTAGC CCTAGTGAAT GATGCCCCAA GAGCTGCCTC CATCGTCTTG
    AGAGAAGATA ACTGCCATTT CTTAAGAGTA GACAAGGAGG ATTTCAACCG GATCCTGAGG
    GACGTTGAGG CGAATACAGT CAGACTTAAA GAACATGACC AAGATGTCTT GGTGCTGGAG
    AAGGTCCCAG CAGGGAACAG GGCTTCTAAT CAAGGAAACT CACAGCCTCA GCAAAAGTAT
    ACTGTGATGT CAGGAACACC TGAAAAAATT TTAGAGCATT TTCTAGAAAC GATACGCCTT
    GAGCCAGCTT TAAATGAAGC AACAGATTCT GTTTTAAATG ACTTTGTTAT GATGCACTGT
    GTTTTTATGC CAAATACCCA GCTTTGCCCA GCTCTGGTGG CCCATTACCA TGCACAGCCT
    TCGCAAGGTA CAGAACAAGA GAAAATGGAT TATGCCCTCA ACAACAAGAG GCGAGTCATC
    CGCCTGGTTT TGCAGTGGGC TGCCATGTAT GGAGACATCC TACAAGAGGA TGACGTGGCC
    ATGGCTTTCC TGGAGGAGTT TTATGTGTCT GTATCAGATG ATGCCCGGAT GCTTGCCGCC
    CTCAAGGAGC AACTGCCTGA GCTGGAGAAG ATTGTCAAGC AAATTTCAGA AGATGCAAAG
    GCGCCACAAA AGAAGCACAA AGTTCTTTTG CAACAGTTCA ACACAGGCGA TGAGAGAGCC
    CAGAAGCGCC AGCCTATCCG TGGCTCTGAT GAAGTTCTGT TTAAAGTCTA TTGCGTGGAC
    CACACCTACA CAACCATCCG GGTACCGGTG GCCGCCTCGG TGAAGGAAGT CCTCAGCGCC
    GTTGCCGACA AACTGGGCTC CGGGGAAGGC CTGATCATCG TCAAGATGAG TTCCGGGGGA
    GAAAAGGTGG TGCTCAAACC TAATGATGTT TCAGTATTTA CAACGCTCAC CATTAATGGA
    CGTCTGTTTG CTTGCCCGCG AGAGCAATTC GATTCACTGA CTCCCTTGCC AGAACAGGAG
    GGCCCAACTG TTGGAACAAT GGGAACTTTC GAACTGATGA GCTCCAAAGA TTTGGCCTAC
    CAGATGACAA TTTATGATTG GGAGCTCTTC AACTGTGTGC ATGAGCTGGA GCTAATCTAT
    CACACATTTG GAAGGCATCA TTTTAAAAAG ACCACAGCAA ACTTGGATTT GTTCCTGAGG
    AGGTTTAATG AAATTCAGTT TTGGGTTGTC ACTGAGATCT GCCTTTGTTC CCAGCCTAGC
    AAGCGTGTTC AGCTCTTAAA AAAATTTATT AAGATCGCAG CCCACTGTAA GGAGTACAAA
    AATCTGAATT CCTTTTTTGC CATCGTCATG GGACTAAGTA ATGTAGCTGT GAGCCGCCTG
    GCACTGACGT GGGAGAAACT GCCAAGCAAG TTCAAGAAGT TCTATGCAGA GTTTGAAAGT
    TTAATGGATC CTTCAAGAAA CCACAGGGCC TACAGGCTGA CAGTAGCCAA ATTGGACCCT
    CCTCTCATCC CCTTCATGCC TTTGCTCATT AAAGATATGA CATTTACTCA TGAGGGGAAC
    AAGACGTTCA TTGACAATCT CGTAAACTTT GAAAAAATGC GAATGATTGC AAATACTGCC
    AGAACAGTGA GATACTGCAG GAGCCAACCC TTCAATCCTG ATGCAGCTCA AGCTAATAAG
    AACCACCAGG ATGTCCGGAG TTACGTTCGG CAATTAAATG TGATCGACAA CCAGAGAACT
    TTATCACAGA TGTCACACAG ACTAGAGCCT CGTCGACCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87900
    Clone ID Related Accession (Same CDS sequence) XM_015474948.1
    Accession Version XM_015474948.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCCCTGCA AGAAATATCG ACAGTACATG GCAGGACTTC TGGCTCCTCC TTATGGTGTT 
    ATGGAAACGG GCTCTAACAA TGACAGGATT CCTGACAAGG AGAACGTTCC TTCAGAGAAG
    ATCCTCAGGG CTGGAAAAAT TTTACGAAAC GCCATTCTCT CTCGAGCACC TCACATGATA
    AGAGATAGAA AATACCACCT AAAGACATAC AGACAGTGCT GTGTGGGGAC CGAGCTGGTG
    GACTGGATGC TGCAGCAGAC TCCATGTGTT CACTCCCGAA CGCAAGCTGT GGGCATGTGG
    CAAGTCCTGG TAGAAGACAG CGTTCTCAAC CATGTGGATC AGGAGCATCA CTTCCAAGAC
    AAATATTTAT TTTATCGGTT TCTGGATGAT GAGCATGAGG ACGCCCCTTT GCCTACGGAG
    GAGGAAAAAA AGGAGTGTGA TGAGGAGCTG CAGGACACCA TGCTGCTGCT GTCGCAGATG
    GGCCCGGACG CCCACATGAG GATGGTCCTT CGCAAACCAC CTGGACAGAG GACCATGGAT
    GACCTAGAAA TTATCTATGA AGAACTCCTT CACATTAAAG CCTTATCCCA TCTTTCTACC
    ACAGTGAAAC GGGAGTTGGC AGCGGTTCTC ATTTTTGAGT CTCATGCCAA AGGAGGGACT
    GTGTTATTTA ACCAAGGGGA AGAAGGCACC TCCTGGTACA TTATTCTAAA AGGATCAGTG
    AATGTAGTCA TTTATGGCAA GGGTGTGGTC TGTACCCTGC ATGAGGGCGA TGACTTTGGC
    AAGTTAGCCC TAGTGAATGA TGCCCCAAGA GCTGCCTCCA TCGTCTTGAG AGAAGATAAC
    TGCCATTTCT TAAGAGTAGA CAAGGAGGAT TTCAACCGGA TCCTGAGGGA CGTTGAGGCG
    AATACAGTCA GACTTAAAGA ACATGACCAA GATGTCTTGG TGCTGGAGAA GGTCCCAGCA
    GGGAACAGGG CTTCTAATCA AGGAAACTCA CAGCCTCAGC AAAAGTATAC TGTGATGTCA
    GGAACACCTG AAAAAATTTT AGAGCATTTT CTAGAAACGA TACGCCTTGA GCCAGCTTTA
    AATGAAGCAA CAGATTCTGT TTTAAATGAC TTTGTTATGA TGCACTGTGT TTTTATGCCA
    AATACCCAGC TTTGCCCAGC TCTGGTGGCC CATTACCATG CACAGCCTTC GCAAGGTACA
    GAACAAGAGA AAATGGATTA TGCCCTCAAC AACAAGAGGC GAGTCATCCG CCTGGTTTTG
    CAGTGGGCTG CCATGTATGG AGACATCCTA CAAGAGGATG ACGTGGCCAT GGCTTTCCTG
    GAGGAGTTTT ATGTGTCTGT ATCAGATGAT GCCCGGATGC TTGCCGCCCT CAAGGAGCAA
    CTGCCTGAGC TGGAGAAGAT TGTCAAGCAA ATTTCAGAAG ATGCAAAGGC GCCACAAAAG
    AAGCACAAAG TTCTTTTGCA ACAGTTCAAC ACAGGCGATG AGAGAGCCCA GAAGCGCCAG
    CCTATCCGTG GCTCTGATGA AGTTCTGTTT AAAGTCTATT GCGTGGACCA CACCTACACA
    ACCATCCGGG TACCGGTGGC CGCCTCGGTG AAGGAAGTCC TCAGCGCCGT TGCCGACAAA
    CTGGGCTCCG GGGAAGGCCT GATCATCGTC AAGATGAGTT CCGGGGGAGA AAAGGTGGTG
    CTCAAACCTA ATGATGTTTC AGTATTTACA ACGCTCACCA TTAATGGACG TCTGTTTGCT
    TGCCCGCGAG AGCAATTCGA TTCACTGACT CCCTTGCCAG AACAGGAGGG CCCAACTGTT
    GGAACAATGG GAACTTTCGA ACTGATGAGC TCCAAAGATT TGGCCTACCA GATGACAATT
    TATGATTGGG AGCTCTTCAA CTGTGTGCAT GAGCTGGAGC TAATCTATCA CACATTTGGA
    AGGCATCATT TTAAAAAGAC CACAGCAAAC TTGGATTTGT TCCTGAGGAG GTTTAATGAA
    ATTCAGTTTT GGGTTGTCAC TGAGATCTGC CTTTGTTCCC AGCCTAGCAA GCGTGTTCAG
    CTCTTAAAAA AATTTATTAA GATCGCAGCC CACTGTAAGG AGTACAAAAA TCTGAATTCC
    TTTTTTGCCA TCGTCATGGG ACTAAGTAAT GTAGCTGTGA GCCGCCTGGC ACTGACGTGG
    GAGAAACTGC CAAGCAAGTT CAAGAAGTTC TATGCAGAGT TTGAAAGTTT AATGGATCCT
    TCAAGAAACC ACAGGGCCTA CAGGCTGACA GTAGCCAAAT TGGACCCTCC TCTCATCCCC
    TTCATGCCTT TGCTCATTAA AGATATGACA TTTACTCATG AGGGGAACAA GACGTTCATT
    GACAATCTCG TAAACTTTGA AAAAATGCGA ATGATTGCAA ATACTGCCAG AACAGTGAGA
    TACTGCAGGA GCCAACCCTT CAATCCTGAT GCAGCTCAAG CTAATAAGAA CCACCAGGAT
    GTCCGGAGTT ACGTTCGGCA ATTAAATGTG ATCGACAACC AGAGAACTTT ATCACAGATG
    TCACACAGAC TAGAGCCTCG TCGACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330434.1
    CDS358..2907
    Translation

    Target ORF information:

    RefSeq Version XM_015474948.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474948.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCCCTGCA AGAAATATCG ACAGTACATG GCAGGACTTC TGGCTCCTCC TTATGGTGTT 
    ATGGAAACGG GCTCTAACAA TGACAGGATT CCTGACAAGG AGAACGTTCC TTCAGAGAAG
    ATCCTCAGGG CTGGAAAAAT TTTACGAAAC GCCATTCTCT CTCGAGCACC TCACATGATA
    AGAGATAGAA AATACCACCT AAAGACATAC AGACAGTGCT GTGTGGGGAC CGAGCTGGTG
    GACTGGATGC TGCAGCAGAC TCCATGTGTT CACTCCCGAA CGCAAGCTGT GGGCATGTGG
    CAAGTCCTGG TAGAAGACAG CGTTCTCAAC CATGTGGATC AGGAGCATCA CTTCCAAGAC
    AAATATTTAT TTTATCGGTT TCTGGATGAT GAGCATGAGG ACGCCCCTTT GCCTACGGAG
    GAGGAAAAAA AGGAGTGTGA TGAGGAGCTG CAGGACACCA TGCTGCTGCT GTCGCAGATG
    GGCCCGGACG CCCACATGAG GATGGTCCTT CGCAAACCAC CTGGACAGAG GACCATGGAT
    GACCTAGAAA TTATCTATGA AGAACTCCTT CACATTAAAG CCTTATCCCA TCTTTCTACC
    ACAGTGAAAC GGGAGTTGGC AGCGGTTCTC ATTTTTGAGT CTCATGCCAA AGGAGGGACT
    GTGTTATTTA ACCAAGGGGA AGAAGGCACC TCCTGGTACA TTATTCTAAA AGGATCAGTG
    AATGTAGTCA TTTATGGCAA GGGTGTGGTC TGTACCCTGC ATGAGGGCGA TGACTTTGGC
    AAGTTAGCCC TAGTGAATGA TGCCCCAAGA GCTGCCTCCA TCGTCTTGAG AGAAGATAAC
    TGCCATTTCT TAAGAGTAGA CAAGGAGGAT TTCAACCGGA TCCTGAGGGA CGTTGAGGCG
    AATACAGTCA GACTTAAAGA ACATGACCAA GATGTCTTGG TGCTGGAGAA GGTCCCAGCA
    GGGAACAGGG CTTCTAATCA AGGAAACTCA CAGCCTCAGC AAAAGTATAC TGTGATGTCA
    GGAACACCTG AAAAAATTTT AGAGCATTTT CTAGAAACGA TACGCCTTGA GCCAGCTTTA
    AATGAAGCAA CAGATTCTGT TTTAAATGAC TTTGTTATGA TGCACTGTGT TTTTATGCCA
    AATACCCAGC TTTGCCCAGC TCTGGTGGCC CATTACCATG CACAGCCTTC GCAAGGTACA
    GAACAAGAGA AAATGGATTA TGCCCTCAAC AACAAGAGGC GAGTCATCCG CCTGGTTTTG
    CAGTGGGCTG CCATGTATGG AGACATCCTA CAAGAGGATG ACGTGGCCAT GGCTTTCCTG
    GAGGAGTTTT ATGTGTCTGT ATCAGATGAT GCCCGGATGC TTGCCGCCCT CAAGGAGCAA
    CTGCCTGAGC TGGAGAAGAT TGTCAAGCAA ATTTCAGAAG ATGCAAAGGC GCCACAAAAG
    AAGCACAAAG TTCTTTTGCA ACAGTTCAAC ACAGGCGATG AGAGAGCCCA GAAGCGCCAG
    CCTATCCGTG GCTCTGATGA AGTTCTGTTT AAAGTCTATT GCGTGGACCA CACCTACACA
    ACCATCCGGG TACCGGTGGC CGCCTCGGTG AAGGAAGTCC TCAGCGCCGT TGCCGACAAA
    CTGGGCTCCG GGGAAGGCCT GATCATCGTC AAGATGAGTT CCGGGGGAGA AAAGGTGGTG
    CTCAAACCTA ATGATGTTTC AGTATTTACA ACGCTCACCA TTAATGGACG TCTGTTTGCT
    TGCCCGCGAG AGCAATTCGA TTCACTGACT CCCTTGCCAG AACAGGAGGG CCCAACTGTT
    GGAACAATGG GAACTTTCGA ACTGATGAGC TCCAAAGATT TGGCCTACCA GATGACAATT
    TATGATTGGG AGCTCTTCAA CTGTGTGCAT GAGCTGGAGC TAATCTATCA CACATTTGGA
    AGGCATCATT TTAAAAAGAC CACAGCAAAC TTGGATTTGT TCCTGAGGAG GTTTAATGAA
    ATTCAGTTTT GGGTTGTCAC TGAGATCTGC CTTTGTTCCC AGCCTAGCAA GCGTGTTCAG
    CTCTTAAAAA AATTTATTAA GATCGCAGCC CACTGTAAGG AGTACAAAAA TCTGAATTCC
    TTTTTTGCCA TCGTCATGGG ACTAAGTAAT GTAGCTGTGA GCCGCCTGGC ACTGACGTGG
    GAGAAACTGC CAAGCAAGTT CAAGAAGTTC TATGCAGAGT TTGAAAGTTT AATGGATCCT
    TCAAGAAACC ACAGGGCCTA CAGGCTGACA GTAGCCAAAT TGGACCCTCC TCTCATCCCC
    TTCATGCCTT TGCTCATTAA AGATATGACA TTTACTCATG AGGGGAACAA GACGTTCATT
    GACAATCTCG TAAACTTTGA AAAAATGCGA ATGATTGCAA ATACTGCCAG AACAGTGAGA
    TACTGCAGGA GCCAACCCTT CAATCCTGAT GCAGCTCAAG CTAATAAGAA CCACCAGGAT
    GTCCGGAGTT ACGTTCGGCA ATTAAATGTG ATCGACAACC AGAGAACTTT ATCACAGATG
    TCACACAGAC TAGAGCCTCG TCGACCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87901
    Clone ID Related Accession (Same CDS sequence) XM_010801910.2
    Accession Version XM_010801910.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2544bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801910.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGATGTCA TCATGGCAGT CTACCCAGCA CCTTCCAGTT TTAGGACACC TCTCATTGAA 
    CCTCACGTTC CTCTTCGTCC TGCTAACACC ATTACCAAGG TTCCTTCAGA GAAGATCCTC
    AGGGCTGGAA AAATTTTACG AAACGCCATT CTCTCTCGAG CACCTCACAT GATAAGAGAT
    AGAAAATACC ACCTAAAGAC ATACAGACAG TGCTGTGTGG GGACCGAGCT GGTGGACTGG
    ATGCTGCAGC AGACTCCATG TGTTCACTCC CGAACGCAAG CTGTGGGCAT GTGGCAAGTC
    CTGGTAGAAG ACAGCGTTCT CAACCATGTG GATCAGGAGC ATCACTTCCA AGACAAATAT
    TTATTTTATC GGTTTCTGGA TGATGAGCAT GAGGACGCCC CTTTGCCTAC GGAGGAGGAA
    AAAAAGGAGT GTGATGAGGA GCTGCAGGAC ACCATGCTGC TGCTGTCGCA GATGGGCCCG
    GACGCCCACA TGAGGATGGT CCTTCGCAAA CCACCTGGAC AGAGGACCAT GGATGACCTA
    GAAATTATCT ATGAAGAACT CCTTCACATT AAAGCCTTAT CCCATCTTTC TACCACAGTG
    AAACGGGAGT TGGCAGCGGT TCTCATTTTT GAGTCTCATG CCAAAGGAGG GACTGTGTTA
    TTTAACCAAG GGGAAGAAGG CACCTCCTGG TACATTATTC TAAAAGGATC AGTGAATGTA
    GTCATTTATG GCAAGGGTGT GGTCTGTACC CTGCATGAGG GCGATGACTT TGGCAAGTTA
    GCCCTAGTGA ATGATGCCCC AAGAGCTGCC TCCATCGTCT TGAGAGAAGA TAACTGCCAT
    TTCTTAAGAG TAGACAAGGA GGATTTCAAC CGGATCCTGA GGGACGTTGA GGCGAATACA
    GTCAGACTTA AAGAACATGA CCAAGATGTC TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC
    AGGGCTTCTA ATCAAGGAAA CTCACAGCCT CAGCAAAAGT ATACTGTGAT GTCAGGAACA
    CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA ACGATACGCC TTGAGCCAGC TTTAAATGAA
    GCAACAGATT CTGTTTTAAA TGACTTTGTT ATGATGCACT GTGTTTTTAT GCCAAATACC
    CAGCTTTGCC CAGCTCTGGT GGCCCATTAC CATGCACAGC CTTCGCAAGG TACAGAACAA
    GAGAAAATGG ATTATGCCCT CAACAACAAG AGGCGAGTCA TCCGCCTGGT TTTGCAGTGG
    GCTGCCATGT ATGGAGACAT CCTACAAGAG GATGACGTGG CCATGGCTTT CCTGGAGGAG
    TTTTATGTGT CTGTATCAGA TGATGCCCGG ATGCTTGCCG CCCTCAAGGA GCAACTGCCT
    GAGCTGGAGA AGATTGTCAA GCAAATTTCA GAAGATGCAA AGGCGCCACA AAAGAAGCAC
    AAAGTTCTTT TGCAACAGTT CAACACAGGC GATGAGAGAG CCCAGAAGCG CCAGCCTATC
    CGTGGCTCTG ATGAAGTTCT GTTTAAAGTC TATTGCGTGG ACCACACCTA CACAACCATC
    CGGGTACCGG TGGCCGCCTC GGTGAAGGAA GTCCTCAGCG CCGTTGCCGA CAAACTGGGC
    TCCGGGGAAG GCCTGATCAT CGTCAAGATG AGTTCCGGGG GAGAAAAGGT GGTGCTCAAA
    CCTAATGATG TTTCAGTATT TACAACGCTC ACCATTAATG GACGTCTGTT TGCTTGCCCG
    CGAGAGCAAT TCGATTCACT GACTCCCTTG CCAGAACAGG AGGGCCCAAC TGTTGGAACA
    ATGGGAACTT TCGAACTGAT GAGCTCCAAA GATTTGGCCT ACCAGATGAC AATTTATGAT
    TGGGAGCTCT TCAACTGTGT GCATGAGCTG GAGCTAATCT ATCACACATT TGGAAGGCAT
    CATTTTAAAA AGACCACAGC AAACTTGGAT TTGTTCCTGA GGAGGTTTAA TGAAATTCAG
    TTTTGGGTTG TCACTGAGAT CTGCCTTTGT TCCCAGCCTA GCAAGCGTGT TCAGCTCTTA
    AAAAAATTTA TTAAGATCGC AGCCCACTGT AAGGAGTACA AAAATCTGAA TTCCTTTTTT
    GCCATCGTCA TGGGACTAAG TAATGTAGCT GTGAGCCGCC TGGCACTGAC GTGGGAGAAA
    CTGCCAAGCA AGTTCAAGAA GTTCTATGCA GAGTTTGAAA GTTTAATGGA TCCTTCAAGA
    AACCACAGGG CCTACAGGCT GACAGTAGCC AAATTGGACC CTCCTCTCAT CCCCTTCATG
    CCTTTGCTCA TTAAAGATAT GACATTTACT CATGAGGGGA ACAAGACGTT CATTGACAAT
    CTCGTAAACT TTGAAAAAAT GCGAATGATT GCAAATACTG CCAGAACAGT GAGATACTGC
    AGGAGCCAAC CCTTCAATCC TGATGCAGCT CAAGCTAATA AGAACCACCA GGATGTCCGG
    AGTTACGTTC GGCAATTAAA TGTGATCGAC AACCAGAGAA CTTTATCACA GATGTCACAC
    AGACTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800212.2
    CDS47..2590
    Translation

    Target ORF information:

    RefSeq Version XM_010801910.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801910.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGATGTCA TCATGGCAGT CTACCCAGCA CCTTCCAGTT TTAGGACACC TCTCATTGAA 
    CCTCACGTTC CTCTTCGTCC TGCTAACACC ATTACCAAGG TTCCTTCAGA GAAGATCCTC
    AGGGCTGGAA AAATTTTACG AAACGCCATT CTCTCTCGAG CACCTCACAT GATAAGAGAT
    AGAAAATACC ACCTAAAGAC ATACAGACAG TGCTGTGTGG GGACCGAGCT GGTGGACTGG
    ATGCTGCAGC AGACTCCATG TGTTCACTCC CGAACGCAAG CTGTGGGCAT GTGGCAAGTC
    CTGGTAGAAG ACAGCGTTCT CAACCATGTG GATCAGGAGC ATCACTTCCA AGACAAATAT
    TTATTTTATC GGTTTCTGGA TGATGAGCAT GAGGACGCCC CTTTGCCTAC GGAGGAGGAA
    AAAAAGGAGT GTGATGAGGA GCTGCAGGAC ACCATGCTGC TGCTGTCGCA GATGGGCCCG
    GACGCCCACA TGAGGATGGT CCTTCGCAAA CCACCTGGAC AGAGGACCAT GGATGACCTA
    GAAATTATCT ATGAAGAACT CCTTCACATT AAAGCCTTAT CCCATCTTTC TACCACAGTG
    AAACGGGAGT TGGCAGCGGT TCTCATTTTT GAGTCTCATG CCAAAGGAGG GACTGTGTTA
    TTTAACCAAG GGGAAGAAGG CACCTCCTGG TACATTATTC TAAAAGGATC AGTGAATGTA
    GTCATTTATG GCAAGGGTGT GGTCTGTACC CTGCATGAGG GCGATGACTT TGGCAAGTTA
    GCCCTAGTGA ATGATGCCCC AAGAGCTGCC TCCATCGTCT TGAGAGAAGA TAACTGCCAT
    TTCTTAAGAG TAGACAAGGA GGATTTCAAC CGGATCCTGA GGGACGTTGA GGCGAATACA
    GTCAGACTTA AAGAACATGA CCAAGATGTC TTGGTGCTGG AGAAGGTCCC AGCAGGGAAC
    AGGGCTTCTA ATCAAGGAAA CTCACAGCCT CAGCAAAAGT ATACTGTGAT GTCAGGAACA
    CCTGAAAAAA TTTTAGAGCA TTTTCTAGAA ACGATACGCC TTGAGCCAGC TTTAAATGAA
    GCAACAGATT CTGTTTTAAA TGACTTTGTT ATGATGCACT GTGTTTTTAT GCCAAATACC
    CAGCTTTGCC CAGCTCTGGT GGCCCATTAC CATGCACAGC CTTCGCAAGG TACAGAACAA
    GAGAAAATGG ATTATGCCCT CAACAACAAG AGGCGAGTCA TCCGCCTGGT TTTGCAGTGG
    GCTGCCATGT ATGGAGACAT CCTACAAGAG GATGACGTGG CCATGGCTTT CCTGGAGGAG
    TTTTATGTGT CTGTATCAGA TGATGCCCGG ATGCTTGCCG CCCTCAAGGA GCAACTGCCT
    GAGCTGGAGA AGATTGTCAA GCAAATTTCA GAAGATGCAA AGGCGCCACA AAAGAAGCAC
    AAAGTTCTTT TGCAACAGTT CAACACAGGC GATGAGAGAG CCCAGAAGCG CCAGCCTATC
    CGTGGCTCTG ATGAAGTTCT GTTTAAAGTC TATTGCGTGG ACCACACCTA CACAACCATC
    CGGGTACCGG TGGCCGCCTC GGTGAAGGAA GTCCTCAGCG CCGTTGCCGA CAAACTGGGC
    TCCGGGGAAG GCCTGATCAT CGTCAAGATG AGTTCCGGGG GAGAAAAGGT GGTGCTCAAA
    CCTAATGATG TTTCAGTATT TACAACGCTC ACCATTAATG GACGTCTGTT TGCTTGCCCG
    CGAGAGCAAT TCGATTCACT GACTCCCTTG CCAGAACAGG AGGGCCCAAC TGTTGGAACA
    ATGGGAACTT TCGAACTGAT GAGCTCCAAA GATTTGGCCT ACCAGATGAC AATTTATGAT
    TGGGAGCTCT TCAACTGTGT GCATGAGCTG GAGCTAATCT ATCACACATT TGGAAGGCAT
    CATTTTAAAA AGACCACAGC AAACTTGGAT TTGTTCCTGA GGAGGTTTAA TGAAATTCAG
    TTTTGGGTTG TCACTGAGAT CTGCCTTTGT TCCCAGCCTA GCAAGCGTGT TCAGCTCTTA
    AAAAAATTTA TTAAGATCGC AGCCCACTGT AAGGAGTACA AAAATCTGAA TTCCTTTTTT
    GCCATCGTCA TGGGACTAAG TAATGTAGCT GTGAGCCGCC TGGCACTGAC GTGGGAGAAA
    CTGCCAAGCA AGTTCAAGAA GTTCTATGCA GAGTTTGAAA GTTTAATGGA TCCTTCAAGA
    AACCACAGGG CCTACAGGCT GACAGTAGCC AAATTGGACC CTCCTCTCAT CCCCTTCATG
    CCTTTGCTCA TTAAAGATAT GACATTTACT CATGAGGGGA ACAAGACGTT CATTGACAAT
    CTCGTAAACT TTGAAAAAAT GCGAATGATT GCAAATACTG CCAGAACAGT GAGATACTGC
    AGGAGCCAAC CCTTCAATCC TGATGCAGCT CAAGCTAATA AGAACCACCA GGATGTCCGG
    AGTTACGTTC GGCAATTAAA TGTGATCGAC AACCAGAGAA CTTTATCACA GATGTCACAC
    AGACTAGAGC CTCGTCGACC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87902
    Clone ID Related Accession (Same CDS sequence) XM_015474949.1
    Accession Version XM_015474949.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACGT TCCTTCAGAG AAGATCCTCA GGGCTGGAAA AATTTTACGA
    AACGCCATTC TCTCTCGAGC ACCTCACATG ATAAGAGATA GAAAATACCA CCTAAAGACA
    TACAGACAGT GCTGTGTGGG GACCGAGCTG GTGGACTGGA TGCTGCAGCA GACTCCATGT
    GTTCACTCCC GAACGCAAGC TGTGGGCATG TGGCAAGTCC TGGTAGAAGA CAGCGTTCTC
    AACCATGTGG ATCAGGAGCA TCACTTCCAA GACAAATATT TATTTTATCG GTTTCTGGAT
    GATGAGCATG AGGACGCCCC TTTGCCTACG GAGGAGGAAA AAAAGGAGTG TGATGAGGAG
    CTGCAGGACA CCATGCTGCT GCTGTCGCAG ATGGGCCCGG ACGCCCACAT GAGGATGGTC
    CTTCGCAAAC CACCTGGACA GAGGACCATG GATGACCTAG AAATTATCTA TGAAGAACTC
    CTTCACATTA AAGCCTTATC CCATCTTTCT ACCACAGTGA AACGGGAGTT GGCAGCGGTT
    CTCATTTTTG AGTCTCATGC CAAAGGAGGG ACTGTGTTAT TTAACCAAGG GGAAGAAGGC
    ACCTCCTGGT ACATTATTCT AAAAGGATCA GTGAATGTAG TCATTTATGG CAAGGGTGTG
    GTCTGTACCC TGCATGAGGG CGATGACTTT GGCAAGTTAG CCCTAGTGAA TGATGCCCCA
    AGAGCTGCCT CCATCGTCTT GAGAGAAGAT AACTGCCATT TCTTAAGAGT AGACAAGGAG
    GATTTCAACC GGATCCTGAG GGACGTTGAG GCGAATACAG TCAGACTTAA AGAACATGAC
    CAAGATGTCT TGGTGCTGGA GAAGGTCCCA GCAGGGAACA GGGCTTCTAA TCAAGGAAAC
    TCACAGCCTC AGCAAAAGTA TACTGTGATG TCAGGAACAC CTGAAAAAAT TTTAGAGCAT
    TTTCTAGAAA CGATACGCCT TGAGCCAGCT TTAAATGAAG CAACAGATTC TGTTTTAAAT
    GACTTTGTTA TGATGCACTG TGTTTTTATG CCAAATACCC AGCTTTGCCC AGCTCTGGTG
    GCCCATTACC ATGCACAGCC TTCGCAAGGT ACAGAACAAG AGAAAATGGA TTATGCCCTC
    AACAACAAGA GGCGAGTCAT CCGCCTGGTT TTGCAGTGGG CTGCCATGTA TGGAGACATC
    CTACAAGAGG ATGACGTGGC CATGGCTTTC CTGGAGGAGT TTTATGTGTC TGTATCAGAT
    GATGCCCGGA TGCTTGCCGC CCTCAAGGAG CAACTGCCTG AGCTGGAGAA GATTGTCAAG
    CAAATTTCAG AAGATGCAAA GGCGCCACAA AAGAAGCACA AAGTTCTTTT GCAACAGTTC
    AACACAGGCG ATGAGAGAGC CCAGAAGCGC CAGCCTATCC GTGGCTCTGA TGAAGTTCTG
    TTTAAAGTCT ATTGCGTGGA CCACACCTAC ACAACCATCC GGGTACCGGT GGCCGCCTCG
    GTGAAGGAAG TCCTCAGCGC CGTTGCCGAC AAACTGGGCT CCGGGGAAGG CCTGATCATC
    GTCAAGATGA GTTCCGGGGG AGAAAAGGTG GTGCTCAAAC CTAATGATGT TTCAGTATTT
    ACAACGCTCA CCATTAATGG ACGTCTGTTT GCTTGCCCGC GAGAGCAATT CGATTCACTG
    ACTCCCTTGC CAGAACAGGA GGGCCCAACT GTTGGAACAA TGGGAACTTT CGAACTGATG
    AGCTCCAAAG ATTTGGCCTA CCAGATGACA ATTTATGATT GGGAGCTCTT CAACTGTGTG
    CATGAGCTGG AGCTAATCTA TCACACATTT GGAAGGCATC ATTTTAAAAA GACCACAGCA
    AACTTGGATT TGTTCCTGAG GAGGTTTAAT GAAATTCAGT TTTGGGTTGT CACTGAGATC
    TGCCTTTGTT CCCAGCCTAG CAAGCGTGTT CAGCTCTTAA AAAAATTTAT TAAGATCGCA
    GCCCACTGTA AGGAGTACAA AAATCTGAAT TCCTTTTTTG CCATCGTCAT GGGACTAAGT
    AATGTAGCTG TGAGCCGCCT GGCACTGACG TGGGAGAAAC TGCCAAGCAA GTTCAAGAAG
    TTCTATGCAG AGTTTGAAAG TTTAATGGAT CCTTCAAGAA ACCACAGGGC CTACAGGCTG
    ACAGTAGCCA AATTGGACCC TCCTCTCATC CCCTTCATGC CTTTGCTCAT TAAAGATATG
    ACATTTACTC ATGAGGGGAA CAAGACGTTC ATTGACAATC TCGTAAACTT TGAAAAAATG
    CGAATGATTG CAAATACTGC CAGAACAGTG AGATACTGCA GGAGCCAACC CTTCAATCCT
    GATGCAGCTC AAGCTAATAA GAACCACCAG GATGTCCGGA GTTACGTTCG GCAATTAAAT
    GTGATCGACA ACCAGAGAAC TTTATCACAG ATGTCACACA GACTAGAGCC TCGTCGACCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330435.1
    CDS391..2913
    Translation

    Target ORF information:

    RefSeq Version XM_015474949.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474949.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGGAC TTCTGGCTCC TCCTTATGGT GTTATGGAAA CGGGCTCTAA CAATGACAGG 
    ATTCCTGACA AGGAGAACGT TCCTTCAGAG AAGATCCTCA GGGCTGGAAA AATTTTACGA
    AACGCCATTC TCTCTCGAGC ACCTCACATG ATAAGAGATA GAAAATACCA CCTAAAGACA
    TACAGACAGT GCTGTGTGGG GACCGAGCTG GTGGACTGGA TGCTGCAGCA GACTCCATGT
    GTTCACTCCC GAACGCAAGC TGTGGGCATG TGGCAAGTCC TGGTAGAAGA CAGCGTTCTC
    AACCATGTGG ATCAGGAGCA TCACTTCCAA GACAAATATT TATTTTATCG GTTTCTGGAT
    GATGAGCATG AGGACGCCCC TTTGCCTACG GAGGAGGAAA AAAAGGAGTG TGATGAGGAG
    CTGCAGGACA CCATGCTGCT GCTGTCGCAG ATGGGCCCGG ACGCCCACAT GAGGATGGTC
    CTTCGCAAAC CACCTGGACA GAGGACCATG GATGACCTAG AAATTATCTA TGAAGAACTC
    CTTCACATTA AAGCCTTATC CCATCTTTCT ACCACAGTGA AACGGGAGTT GGCAGCGGTT
    CTCATTTTTG AGTCTCATGC CAAAGGAGGG ACTGTGTTAT TTAACCAAGG GGAAGAAGGC
    ACCTCCTGGT ACATTATTCT AAAAGGATCA GTGAATGTAG TCATTTATGG CAAGGGTGTG
    GTCTGTACCC TGCATGAGGG CGATGACTTT GGCAAGTTAG CCCTAGTGAA TGATGCCCCA
    AGAGCTGCCT CCATCGTCTT GAGAGAAGAT AACTGCCATT TCTTAAGAGT AGACAAGGAG
    GATTTCAACC GGATCCTGAG GGACGTTGAG GCGAATACAG TCAGACTTAA AGAACATGAC
    CAAGATGTCT TGGTGCTGGA GAAGGTCCCA GCAGGGAACA GGGCTTCTAA TCAAGGAAAC
    TCACAGCCTC AGCAAAAGTA TACTGTGATG TCAGGAACAC CTGAAAAAAT TTTAGAGCAT
    TTTCTAGAAA CGATACGCCT TGAGCCAGCT TTAAATGAAG CAACAGATTC TGTTTTAAAT
    GACTTTGTTA TGATGCACTG TGTTTTTATG CCAAATACCC AGCTTTGCCC AGCTCTGGTG
    GCCCATTACC ATGCACAGCC TTCGCAAGGT ACAGAACAAG AGAAAATGGA TTATGCCCTC
    AACAACAAGA GGCGAGTCAT CCGCCTGGTT TTGCAGTGGG CTGCCATGTA TGGAGACATC
    CTACAAGAGG ATGACGTGGC CATGGCTTTC CTGGAGGAGT TTTATGTGTC TGTATCAGAT
    GATGCCCGGA TGCTTGCCGC CCTCAAGGAG CAACTGCCTG AGCTGGAGAA GATTGTCAAG
    CAAATTTCAG AAGATGCAAA GGCGCCACAA AAGAAGCACA AAGTTCTTTT GCAACAGTTC
    AACACAGGCG ATGAGAGAGC CCAGAAGCGC CAGCCTATCC GTGGCTCTGA TGAAGTTCTG
    TTTAAAGTCT ATTGCGTGGA CCACACCTAC ACAACCATCC GGGTACCGGT GGCCGCCTCG
    GTGAAGGAAG TCCTCAGCGC CGTTGCCGAC AAACTGGGCT CCGGGGAAGG CCTGATCATC
    GTCAAGATGA GTTCCGGGGG AGAAAAGGTG GTGCTCAAAC CTAATGATGT TTCAGTATTT
    ACAACGCTCA CCATTAATGG ACGTCTGTTT GCTTGCCCGC GAGAGCAATT CGATTCACTG
    ACTCCCTTGC CAGAACAGGA GGGCCCAACT GTTGGAACAA TGGGAACTTT CGAACTGATG
    AGCTCCAAAG ATTTGGCCTA CCAGATGACA ATTTATGATT GGGAGCTCTT CAACTGTGTG
    CATGAGCTGG AGCTAATCTA TCACACATTT GGAAGGCATC ATTTTAAAAA GACCACAGCA
    AACTTGGATT TGTTCCTGAG GAGGTTTAAT GAAATTCAGT TTTGGGTTGT CACTGAGATC
    TGCCTTTGTT CCCAGCCTAG CAAGCGTGTT CAGCTCTTAA AAAAATTTAT TAAGATCGCA
    GCCCACTGTA AGGAGTACAA AAATCTGAAT TCCTTTTTTG CCATCGTCAT GGGACTAAGT
    AATGTAGCTG TGAGCCGCCT GGCACTGACG TGGGAGAAAC TGCCAAGCAA GTTCAAGAAG
    TTCTATGCAG AGTTTGAAAG TTTAATGGAT CCTTCAAGAA ACCACAGGGC CTACAGGCTG
    ACAGTAGCCA AATTGGACCC TCCTCTCATC CCCTTCATGC CTTTGCTCAT TAAAGATATG
    ACATTTACTC ATGAGGGGAA CAAGACGTTC ATTGACAATC TCGTAAACTT TGAAAAAATG
    CGAATGATTG CAAATACTGC CAGAACAGTG AGATACTGCA GGAGCCAACC CTTCAATCCT
    GATGCAGCTC AAGCTAATAA GAACCACCAG GATGTCCGGA GTTACGTTCG GCAATTAAAT
    GTGATCGACA ACCAGAGAAC TTTATCACAG ATGTCACACA GACTAGAGCC TCGTCGACCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40485
    Clone ID Related Accession (Same CDS sequence) NM_001192902.1
    Accession Version NM_001192902.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000002.1. On Jul 17, 2010 this sequence version replaced XM_617471.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571254.15827.1, SRR5571254.17134.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCGGCCG AGTGGATCGC CTGTCTGGAT 
    AAAAGACCAC TGGAACGATC CAGTGAAGAT GTGGATATAA TCTTCACACG GCTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGTTT ATGTGGTTAT
    TATGAGAATC TGGAAAAAGG AATAACATTA TTTCGCCAAG GTGATATTGG AACCAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTTTCTG AGACCAGCAG TCACCAGGAC
    GCAGTGACCA TCTGCACTCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACCCCACGCC ATGCGACCAT CGTTACCAGG GAGAGCAGTG AGCTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTACA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTTCCTTCA
    GAGAAGATCC TCAGGGCTGG AAAAATTTTA CGAAACGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AGTGCTGTGT GGGGACCGAG
    CTGGTGGACT GGATGCTGCA GCAGACTCCA TGTGTTCACT CCCGAACGCA AGCTGTGGGC
    ATGTGGCAAG TCCTGGTAGA AGACAGCGTT CTCAACCATG TGGATCAGGA GCATCACTTC
    CAAGACAAAT ATTTATTTTA TCGGTTTCTG GATGATGAGC ATGAGGACGC CCCTTTGCCT
    ACGGAGGAGG AAAAAAAGGA GTGTGATGAG GAGCTGCAGG ACACCATGCT GCTGCTGTCG
    CAGATGGGCC CGGACGCCCA CATGAGGATG GTCCTTCGCA AACCACCTGG ACAGAGGACC
    ATGGATGACC TAGAAATTAT CTATGAAGAA CTCCTTCACA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGGGA GTTGGCAGCG GTTCTCATTT TTGAGTCTCA TGCCAAAGGA
    GGGACTGTGT TATTTAACCA AGGGGAAGAA GGCACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA TGGCAAGGGT GTGGTCTGTA CCCTGCATGA GGGCGATGAC
    TTTGGCAAGT TAGCCCTAGT GAATGATGCC CCAAGAGCTG CCTCCATCGT CTTGAGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT GAGGGACGTT
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGGGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACGATACG CCTTGAGCCA
    GCTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTG TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCAGCTCTG GTGGCCCATT ACCATGCACA GCCTTCGCAA
    GGTACAGAAC AAGAGAAAAT GGATTATGCC CTCAACAACA AGAGGCGAGT CATCCGCCTG
    GTTTTGCAGT GGGCTGCCAT GTATGGAGAC ATCCTACAAG AGGATGACGT GGCCATGGCT
    TTCCTGGAGG AGTTTTATGT GTCTGTATCA GATGATGCCC GGATGCTTGC CGCCCTCAAG
    GAGCAACTGC CTGAGCTGGA GAAGATTGTC AAGCAAATTT CAGAAGATGC AAAGGCGCCA
    CAAAAGAAGC ACAAAGTTCT TTTGCAACAG TTCAACACAG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGTGGCTC TGATGAAGTT CTGTTTAAAG TCTATTGCGT GGACCACACC
    TACACAACCA TCCGGGTACC GGTGGCCGCC TCGGTGAAGG AAGTCCTCAG CGCCGTTGCC
    GACAAACTGG GCTCCGGGGA AGGCCTGATC ATCGTCAAGA TGAGTTCCGG GGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACAACGC TCACCATTAA TGGACGTCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TGCCAGAACA GGAGGGCCCA
    ACTGTTGGAA CAATGGGAAC TTTCGAACTG ATGAGCTCCA AAGATTTGGC CTACCAGATG
    ACAATTTATG ATTGGGAGCT CTTCAACTGT GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATCATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGGTTT
    AATGAAATTC AGTTTTGGGT TGTCACTGAG ATCTGCCTTT GTTCCCAGCC TAGCAAGCGT
    GTTCAGCTCT TAAAAAAATT TATTAAGATC GCAGCCCACT GTAAGGAGTA CAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAATGTAG CTGTGAGCCG CCTGGCACTG
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CAGAGTTTGA AAGTTTAATG
    GATCCTTCAA GAAACCACAG GGCCTACAGG CTGACAGTAG CCAAATTGGA CCCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACG
    TTCATTGACA ATCTCGTAAA CTTTGAAAAA ATGCGAATGA TTGCAAATAC TGCCAGAACA
    GTGAGATACT GCAGGAGCCA ACCCTTCAAT CCTGATGCAG CTCAAGCTAA TAAGAACCAC
    CAGGATGTCC GGAGTTACGT TCGGCAATTA AATGTGATCG ACAACCAGAG AACTTTATCA
    CAGATGTCAC ACAGACTAGA GCCTCGTCGA CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179831.1
    CDS1..3036
    Translation

    Target ORF information:

    RefSeq Version NM_001192902.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 4 (RAPGEF4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192902.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGTCGCTG CGCACGCTGC CCATTCTTCC TCCTCGGCCG AGTGGATCGC CTGTCTGGAT 
    AAAAGACCAC TGGAACGATC CAGTGAAGAT GTGGATATAA TCTTCACACG GCTGAAAGAA
    GTTAAAGCTT TTGAGAAATT TCACCCAAAT CTCCTTCATC AGATTTGTTT ATGTGGTTAT
    TATGAGAATC TGGAAAAAGG AATAACATTA TTTCGCCAAG GTGATATTGG AACCAACTGG
    TATGCTGTCC TGGCAGGGTC TTTGGATGTT AAAGTTTCTG AGACCAGCAG TCACCAGGAC
    GCAGTGACCA TCTGCACTCT GGGAATTGGG ACGGCCTTTG GAGAGTCCAT TCTGGACAAC
    ACCCCACGCC ATGCGACCAT CGTTACCAGG GAGAGCAGTG AGCTGCTCCG CATCGAGCAG
    AAGGACTTCA AGGCACTATG GGAGAAATAT CGACAGTACA TGGCAGGACT TCTGGCTCCT
    CCTTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCTGACAA GGAGAACACA
    CCTCTCATTG AACCTCACGT TCCTCTTCGT CCTGCTAACA CCATTACCAA GGTTCCTTCA
    GAGAAGATCC TCAGGGCTGG AAAAATTTTA CGAAACGCCA TTCTCTCTCG AGCACCTCAC
    ATGATAAGAG ATAGAAAATA CCACCTAAAG ACATACAGAC AGTGCTGTGT GGGGACCGAG
    CTGGTGGACT GGATGCTGCA GCAGACTCCA TGTGTTCACT CCCGAACGCA AGCTGTGGGC
    ATGTGGCAAG TCCTGGTAGA AGACAGCGTT CTCAACCATG TGGATCAGGA GCATCACTTC
    CAAGACAAAT ATTTATTTTA TCGGTTTCTG GATGATGAGC ATGAGGACGC CCCTTTGCCT
    ACGGAGGAGG AAAAAAAGGA GTGTGATGAG GAGCTGCAGG ACACCATGCT GCTGCTGTCG
    CAGATGGGCC CGGACGCCCA CATGAGGATG GTCCTTCGCA AACCACCTGG ACAGAGGACC
    ATGGATGACC TAGAAATTAT CTATGAAGAA CTCCTTCACA TTAAAGCCTT ATCCCATCTT
    TCTACCACAG TGAAACGGGA GTTGGCAGCG GTTCTCATTT TTGAGTCTCA TGCCAAAGGA
    GGGACTGTGT TATTTAACCA AGGGGAAGAA GGCACCTCCT GGTACATTAT TCTAAAAGGA
    TCAGTGAATG TAGTCATTTA TGGCAAGGGT GTGGTCTGTA CCCTGCATGA GGGCGATGAC
    TTTGGCAAGT TAGCCCTAGT GAATGATGCC CCAAGAGCTG CCTCCATCGT CTTGAGAGAA
    GATAACTGCC ATTTCTTAAG AGTAGACAAG GAGGATTTCA ACCGGATCCT GAGGGACGTT
    GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTGCT GGAGAAGGTC
    CCAGCAGGGA ACAGGGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG
    ATGTCAGGAA CACCTGAAAA AATTTTAGAG CATTTTCTAG AAACGATACG CCTTGAGCCA
    GCTTTAAATG AAGCAACAGA TTCTGTTTTA AATGACTTTG TTATGATGCA CTGTGTTTTT
    ATGCCAAATA CCCAGCTTTG CCCAGCTCTG GTGGCCCATT ACCATGCACA GCCTTCGCAA
    GGTACAGAAC AAGAGAAAAT GGATTATGCC CTCAACAACA AGAGGCGAGT CATCCGCCTG
    GTTTTGCAGT GGGCTGCCAT GTATGGAGAC ATCCTACAAG AGGATGACGT GGCCATGGCT
    TTCCTGGAGG AGTTTTATGT GTCTGTATCA GATGATGCCC GGATGCTTGC CGCCCTCAAG
    GAGCAACTGC CTGAGCTGGA GAAGATTGTC AAGCAAATTT CAGAAGATGC AAAGGCGCCA
    CAAAAGAAGC ACAAAGTTCT TTTGCAACAG TTCAACACAG GCGATGAGAG AGCCCAGAAG
    CGCCAGCCTA TCCGTGGCTC TGATGAAGTT CTGTTTAAAG TCTATTGCGT GGACCACACC
    TACACAACCA TCCGGGTACC GGTGGCCGCC TCGGTGAAGG AAGTCCTCAG CGCCGTTGCC
    GACAAACTGG GCTCCGGGGA AGGCCTGATC ATCGTCAAGA TGAGTTCCGG GGGAGAAAAG
    GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACAACGC TCACCATTAA TGGACGTCTG
    TTTGCTTGCC CGCGAGAGCA ATTCGATTCA CTGACTCCCT TGCCAGAACA GGAGGGCCCA
    ACTGTTGGAA CAATGGGAAC TTTCGAACTG ATGAGCTCCA AAGATTTGGC CTACCAGATG
    ACAATTTATG ATTGGGAGCT CTTCAACTGT GTGCATGAGC TGGAGCTAAT CTATCACACA
    TTTGGAAGGC ATCATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGGTTT
    AATGAAATTC AGTTTTGGGT TGTCACTGAG ATCTGCCTTT GTTCCCAGCC TAGCAAGCGT
    GTTCAGCTCT TAAAAAAATT TATTAAGATC GCAGCCCACT GTAAGGAGTA CAAAAATCTG
    AATTCCTTTT TTGCCATCGT CATGGGACTA AGTAATGTAG CTGTGAGCCG CCTGGCACTG
    ACGTGGGAGA AACTGCCAAG CAAGTTCAAG AAGTTCTATG CAGAGTTTGA AAGTTTAATG
    GATCCTTCAA GAAACCACAG GGCCTACAGG CTGACAGTAG CCAAATTGGA CCCTCCTCTC
    ATCCCCTTCA TGCCTTTGCT CATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACG
    TTCATTGACA ATCTCGTAAA CTTTGAAAAA ATGCGAATGA TTGCAAATAC TGCCAGAACA
    GTGAGATACT GCAGGAGCCA ACCCTTCAAT CCTGATGCAG CTCAAGCTAA TAAGAACCAC
    CAGGATGTCC GGAGTTACGT TCGGCAATTA AATGTGATCG ACAACCAGAG AACTTTATCA
    CAGATGTCAC ACAGACTAGA GCCTCGTCGA CCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.