WDFY3 cDNA ORF clone, Bos taurus(cattle)

The following WDFY3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WDFY3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa235606 XM_015471703.2
Latest version!
Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa90115 XM_010797980.2
Latest version!
Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa90115 XM_015471704.1
Latest version!
Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa90115 XM_015463824.1
Latest version!
Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa90115 XM_015471703.1
Latest version!
Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa90116 XM_015463825.1
Latest version!
Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa90116 XM_015471705.1
Latest version!
Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa90117 XM_015471706.1
Latest version!
Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa90117 XM_015463826.1
Latest version!
Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa235606
    Clone ID Related Accession (Same CDS sequence) XM_015471703.2
    Accession Version XM_015471703.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product WD repeat and FYVE domain-containing protein 3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471703.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    ATGAACATGG TGAAGCGGAT CATGGGGCGG CCGAGGCAGG AGGAATGCAG CCCGCAAGAT 
    AACGCCCTAG GACTGATGCA CCTCCGCAGG CTCTTCACAG AGCTGTGCCA CCCTCCTCGG
    CACATGACCC AGAAGGAACA AGAAGAGAAG CTGTACATGA TGCTGCCAGT GTTTAACAGG
    GTTTTTGGAA ACGCTCCGCC AAATACAATG ACAGAAAAAT TTTCTGATCT GCTGCAGTTC
    ACAACACAAG TCTCACGACT GATGGTGACA GAAATTCGAA GGAGAGCATC AAACAAATCC
    ACAGAGGCTG CAAGTCGGGC CATAGTCCAG TTCTTGGAAA TCAATCAGAG TGAAGAAGCC
    AGTAGAGGTT GGATGCTTCT GACAACAATT AATTTGTTAG CATCCTCTGG TCAGAAAACC
    GTGGACTGCA TGACAACAAT GTCAGTGCCT TCCACCCTGG TTAAATGTTT ATATCTGTTT
    TTTGACCTTC CACATGTGCC TGAGGCAGTT GGAGGTGCAC AGAATGAGCT ACCTCTAGCA
    GAACGTCGGG GACTACTCCA GAAAGTTTTT GTACAGATCT TAGTGAAACT CTGCAGTTTT
    GTTTCTCCGG CCGAGGAGCT GGCTCAGAAA GACGATCTCC AGCTTTTATT CAGTGCAATA
    ACCTCTTGGT GCCCTCCCTA TAACCTGCCT TGGAGGAAGA GTGCCGGAGA AGTCCTTATG
    ACCATATCCC GTCATGGTCT CAGTGTCAAC GTGGTGAAGT ATATTCACGA AAAAGAATGT
    TTATCTACGT GTGTTCAGAA TATGCAGCAG TCAGATGACC TGTCTCCCCT AGAAATTGTT
    GAGATGTTTG CTGGGCTTTC TTGTTTCCTC AAAGATTCCA GTGATGTTTC CCAAACTCTT
    CTGGATGATT TTCGGATATG GCAAGGATAT AATTTCCTTT GTGATCTCTT GCTTAGATTG
    GAACAAGCAA AAGAGGCAGA ATCCAAAGAT GCCTTGAAAG ATCTGGTTAA TCTGATAACT
    TCCCTAACAA CATATGGTGT CAATGAACTA AAACCAGCTG GTATTACCAC AGGGGCACCC
    TTTTTATTGC CTGGATTTGC AGTACCTCAG CCTGCGGGCA AAGGTCACAG TGTGAGAAAC
    ATCCAGGCCT TTGCAGTTCT TCAAAATGCA TTTTTAAAAG CAAAAACCAA CTTCCTCGCC
    CACATCATTC TCGATGCCAT CACAAATATT TACATGGCTG ACAACGCCAA CTATTTCATC
    CTCGAATCAC AGCACACACT GTCACAGTTT GCAGAAAAAA TTTCTAAACT CCCAGAAGTA
    CAAAACAAGT ACTTCGAGAT GCTGGAGTTT GTTGTTTTTA GCTTAAATTA TATCCCTTGT
    AAAGAACTTA TTAGTGTTAG CATCCTCTTA AAGTCTAGCT CTTCTTATCA CTGTAGCATT
    ATCGCGATGA AAACACTTCT TAAGTTTACA CGACATGACT ACATATTTAA AGATGTGTTC
    AGGGAGGTGG GCCTTTTGGA GGTCATGGTA AACCTTTTGC ACAAATATGC TGCCCTCTTG
    AAGGATCCAA CTCAGGCACT AAATGAACAA GGGGACTCAA GAAATAATAG TTCATTTGAA
    GACCAAAAAC ACCTGGCTTT GTTAGTCATG GAGACTTTGA CAGTGCTTCT TCAAGGGTCA
    AACACAAACG CAGGAATTTT CCGAGAATTT GGAGGCGCAA GATGTGCGCA TAATATAGTG
    AGGTACCCAC AGTGTCGGCA GCATGCCCTG ATGATGATCC AGCAGTTGGT GCTGTCTCCC
    AATGGGGACG ATGACATGGG CACGCTTCTG GGACTCATGC ACTCGGCCCC ACCCACAGAG
    CTGCAGCTGA AGACCGACAT CCTCAGAGCC CTCCTGTCGG TCCTTCGAGA AAGTCACCGT
    TCCAGAACAG TCTTTAGGAA GGTTGGAGGC TTTGTGTACG TTACGTCCTT GCTCGTGGCT
    ATGGAAAGGT CTTTGAGTGC GCCCCCCAAG AATGGGTGGG AGAAAGTGAG CCAGGGCCAC
    GTGTTTGAAC TTCTCCACAC CGTGTTCTGC ACACTGACTG CAGCGATGCG TTATGAGCCA
    GCCAACTCAC ACTTCTTCAA AACAGAGATT CAGTATGAGA AGCTGGCAGA CGCTGTTCGA
    TTTCTTGGCT GCTTCTCAGA CCTAAGAAAG ATCAGCCCCA TGAATGTCTT CCCTTCAAAT
    ACTCAGCCAT TTCAAAGACT TTTAGAGGAA GACGTCATCT CGATGGACTC GGTGTCACCC
    ACTCTGCGGC ACTGCAGTAA ACTCTTTATT TATCTCTACA GAGTAGCCAC AGATTCTTTT
    GACAGTCGTG CAGAACAGAT CCCTCCTTGC CTGACAAGTG AGTCTTCTCT TCCCTCTCCT
    TGGGGGACTC CAGCTTTGTC CAGGAAAAGG CACGCGTATC ATTCTGTTTC TACTCCCCCC
    GTCTACCCTC CTAAAAACGT TGCTGACCTG AAACCACACG TGGCAACTTC ATCTCTCTCA
    AGTTCTGATG CCGTCATCAT CCATCCTGGA GCCATGCTTG CTATGCTGGA TCTCCTGGCC
    TCTGTTGGGT CAGTGACACA GCCAGAACAT GCCTTGGACC TTCAACTTGC CGTGGCCAAT
    ATTTTACAAT CCCTGGTGCA CACGGAAAGA AACCAGCAGG TCATGTGTGA AGCTGGTCTC
    CATGCACGAC TGCTGCAGCG GTGCAGTGCC GCTCTGGCTG ACGAGGACCA CTCGCTGCAC
    CCACCCCTGC AGCGCATGTT CGAGCGATTA GCCTCTCAAG CGCTGGAGCC CATGGTGCTC
    AGGGAGTTTT TGCGTTTGGC AAGTCCATTA AATTGTGGTG CCTGGGACAA AAAACTGCTA
    AAACAATATA GGGTCCACAA ACCAAGTTCA CTGAGTTTTG AGCCAGAGAT GAGAAGCAGT
    ATGATCACAT CCATGGAAGG TCTGGGTTCT GATAATGTTT TCAGCTTACA TGAAGATAAC
    CATTACCGGA TAAGCAAGAG CCTAGTGAAA TCTGCAGAAG GAAGTACTGT ACCCCTAACC
    AGAGTGAAAT GTCTGGTCTC CATGACAACC CCACATGACA TCAGACTTCA TGGGTCATCA
    GTTACTCCAG CTTTTGTTGA ATTTGACACA TCCCTTGAAG GGTTTGGCTG CCTGTTCCTG
    CCCAGTCTGG CCCCTCATAA TGCCCCTACA AACAATGCTG TCACAACAGG TCTTATCGAT
    GGGGCCGTGG TCAGCGGTAT TGGTTCTGGT GAACGATTCT TCCCTCCTCC ATCTGGCTTA
    AGCTACTCCA GCTGGTTTTG CATTGAACAT TTTAGTTCTC CTCCCAGTAA CCACCCAGTC
    AGACTCCTTA CTGTTGTTCG CCGAGCAAAT TCTTCTGAGC ACCATTATGT ATGCCTTGCC
    ATAGTTCTCT CTGCAAAAGA TCGGTCTCTG ATTGTCTCCA CCAAAGAGGA ACTACTCCAA
    AATTATGTTG ATGATTTTAG TGAAGAATCC TCTTTCTATG AGATTCTGCC ATGCTGTGCT
    CGCTTCCGTT GTGGAGAACT GATCGTTGAG GGACAGTGGC ATCACCTGGT TCTGGTGATG
    AGCAAAGGGA TGCTGAAGAA CAGCACTGCA ACCCTCTACA TCGACGGCCA GCTTGTCAGC
    ACTGTGAAGC TTCATTATGT TCACAGTACT CCCGGGGGCT CAGGCTCTGC AAACCCACCA
    GTGGTTAGCA CAGTCTATGC CTACATTGGT ACTCCGCCTG CCCAGCGCCA AATTGCTTCA
    TTGGTTTGGC GCCTGGGACC TACACACTTT CTAGAAGAAG TATTACCTCC TTCAAATGTT
    ACTACCATTT ATGAACTAGG ACCAAATTAC GTGGGAAGCT TTCAGGCCGT ATGTATGCCA
    TGTAAAGATG CAAAATCTGA AGGTGTGGTC CCATCTCCTG TGTCATTAGT ACCAGAGGAA
    AAGGTGTCAT TCGGCCTCTA TGCACTGTCC GTGTCCGCCT TGACAGTGGC GAGAATCCGG
    AAGGTGTACA ACAAGCTGGA CAGCAAAGCC ATCGCTAAGC AGTTAGGCAT TTCCTCCCAT
    GAGAACGCCA CTCCTGTGAA GCTGATCCAC AACTCCGCAG GACACCTCAA TGGGCCCGCA
    CGGACAATCG GGGCCAGTCT GATTGGATAC TTGGGAGTAA GAACATTTGT TCCTAAGCCT
    GTTGCCACTA CTTTGCAGTA CGTTGGTGGG GCCGCAGCCA TCCTGGGCCT GGTAGCCATG
    GCTTCTGATG TGGAAGGGCT GTATGCAGCG GTCAAGGCCC TAGTTTGTGT GGTCAAGAGT
    AACCCACTAG CCAGCAAAGA AATGGAAAGA ATCCGGGGCT ACCAGTTGCT GGCAATGCTG
    TTTAAGAAGA AACGCTCCCT TCTTAACAGC CACATTCTCC ATCTCACTTT TTCCTTGGTG
    GGAACTGTTG ACAGTGGACA TGAGACTTCC ATTATTCCAA ATTCAACAGC TTTCCAGGAT
    CTACTTTGTG ATTTTGAAGT CTGGCTCCAT GCTCCATATG AACTTCATCT CTCCCTATTT
    GAACACTTTA TTGAATTGCT CACAGAGTCC AGTGAAGCTT CAAAGAATGC CAAGTTGATG
    AGGGAATTCC AGCTAATCCC AAAGCTGCTA CTGACTCTTC GAGATATGTC TCTATCCCAG
    CCTACCATTG CTGCTATTAG TAACGTGCTG AGCTTCTTAC TGCAGGGTTT TCCCAACAGC
    AATGATCTAC TCAGGTTTGG CCAGTTCATT TCTTCTACTT TACCAACCTT TGCAGTTTGT
    GAGAAGTTTG TAGTTATGGA AATAAACCAC GAAGAGAAGC CTGACACTGG AACTGAAGAA
    GAGTTTGGAG GTCTTGTATC TGCTAATCTT ATACTTTTGA GGAACAGACT TCTGGATATC
    TTACTAAAAC TGGTTTACAC ATCTAAAGAA AAGACAAGCA TTAATTTGCA AGCTTGTGAA
    GAGCTGGTCA GGACGCTGGG CTTTGACTGG ATTATGATGT TTATGGAAGA ACACTTGCAT
    TCTACCACAG TCACAGCAGC CATGAGGATT CTTGTTGTCC TACTGAGTAA TCAGTCTATT
    CTCATCAAGT TCAAGGAGGG ACTCAGTGGT GGAGGCTGGC TGGAGCAGAC AGATTCTGTC
    TTAACGAATA AGATCGGAAC TGTATTAGGA TTCAATGTGG GCAGGAGTGC TGGCGGACGA
    TCAACTGTCA GGGAGATTAA CCGGGATGCT TGTCACTTTC CTGGCTTTCC GGTGCTTCAG
    TCATTCCTTC CTAAACACAC TAATGTCCCT GCTCTCTATT TCCTCCTCAT GGCATTGTTT
    CTGCAGCAGC CCGTTAGTGA GCTGCCTGAG AACCTGCAGG TCAGTGTGCC TGTCGTCAGC
    AGCCGATGTA AGCAGGGTTG CCAGTTTGAT TTAGATTCCA TCTGGACCTT TATCTTTGGA
    GTTCCTGCCT CCAGTGGAAC CGTGGTCTCA TCTATCCACA ATGTGTGCAC AGAAGCTGCT
    TTTTTGTTAC TGGGCATGCT CCGAAGCATG CTGAATTCAC CTTGGCAATC AGAAGAAGAG
    GGGTCTTGGC TCCGGGAGTA TCCTGTGACC CTGATGCAGT TCTTCAGATA CTTGTATCAC
    AATGTCCCGG ACCTGGCCTC CATGTGGATG AACCCTGATT TCCTGTGTGC ACTTGCAGCC
    ACGGTCTTCC CCTTCAACAT CCGCCCTTAT TCAGAAATGG TGACTGATCT CGATGATGAA
    GTTGGATCTC CAGCTGAAGA ATTTAAAGCC TTTGCAGCAG ACACAGGGAT GAACAGAAGC
    CAATCAGAGT ACTGCAACGT GGGCGTCAAG ACATACCTGA CTAATCACCC AGCTAAGAAA
    TTTGTTTTTG ACTTCATGCG GGTCTTAATA ATAGACAACC TCTGTCTTAC ACCTGCCAGC
    AAGCAGACTC CACTGATTGA TCTTTTGTTG GAGGCTTCTC CTGAGAGATC GACAAGAACG
    CAGCAAAAAG AATTTCAGAC TTACATTTTG GATAGTGTGA TGGATCATTT GCTTGCAGCT
    GATGTTTTAC TGGGAGAAGA TGCATCTCTT CCTATCACCA GTGGAGGAAG CTACCAGGTA
    TTGGTGAACA ATGTGTTTTA TTTCACACAA CGTGTGGTGG ACAAGCTTTG GCAAGGCATG
    TTCAACAAAG AATCTAAACT TCTTATAGAT TTTATAATTC AACTAATTGC ACAGTCAAAG
    AGAAGATCCC AGGGGTTGTC ACTGGATGCA GTCTATCACT GCCTCAACAG GACTATCCTG
    TACCAGTTCT CCCGGGCGCA CAAAACCGTC CCGCAGCAAG TAGCCCTTCT TGACTCTCTC
    AGGGTCCTGA CTGTCAACAG GAACTTGATC CTGGGCCCCG GAAACCACGA CCAAGAATTC
    ATCAGCTGTC TGGCTCACTG CCTCATTAAC CTGCATGTTG GGAGCAATGT GGATGGATTT
    GGACTGGAAG CAGAAGCCCG CATGACTACA TGGCATATTA TGATCCCCTC GGACATTGAA
    CCAGATGGTG GTTACAGCCA AGATATTAGT GAAGGGCGTC AGCTTCTCAT AAAAGCTGTC
    AACAGAGTTT GGACGGAATT AATTCATAGT AAGAAACAAG TCTTGGAGGA GCTTTTCAAA
    GTCACTCTAC CTGTGAATGA AAGAGGCCAC GTGGACATAG CTATAGCAAG GCCGCTCATC
    GAAGAAGCTA GTTTGAAGTG CTGGCAGAAT CATCTAGCCC ATGAAAAGAA GTGCATAAGT
    CGAGGAGAAG CTTTAATGCC CACCACACAA TCCAAATTGT CCCGTGTCAG CAGTGGCTTT
    GGTCTTTCCA AGTTAACGGG ATCAAGAAGG AATCGAAAGG AAAGCGGGCT TCATAAACAC
    AGCCTTTCCA CCCAGGAGAT TTCTCAGTGG ATGTTTACAC ACATCGCTGT TGTTCGTGAC
    TTGGTAGACA CACAATATAA GGAATACCAG GAGCGTCAGC AAAATGCCCT GAAGTACGTG
    ACGGAAGAGT GGTGCCAGAT CGAGTATGAG CTGCTGCGGG AGCGGGGACT CTGGGGCCCC
    CCCATTGGTT CCCATCTCGA CAAGTGGATG TTGGAGATGA CAGAGGGTCC CTGCAGAATG
    AGGAAAAAAA TGGTGCGAAA TGATATGTTT TATAACCATT ACCCTTACGT GCCAGAAATG
    GAGCAGGAAA CCAACGTGGC GTCTGAGATC CCAAGTAAAC AGCCTGAGAC ACCTGATGAT
    ATCATTCCTC AAAAGAAGCC TGCTCGATAC AGAAGAGCCG TAAGTTATGA CAGTAAAGAA
    TACTACATGC GACTAGCCTC CGGCAACCCA GCCATTGTGC AGGATGCCAT CGTAGAGAGC
    TCTGAAGGAG AAGCTGCTCA GCAAGAACCA GAACATGGTG AAGACACGAT TGCTAAAGTC
    AAAGGACTGG TCAAGCCACC TCTCAAACGG TCTCGGTCAG CACCGGACGG AGGCGATGAG
    GAGACACAGG AGCAGTTACA GGACCAGACA GCTGAGGGCA GCTCGATAGA AGAGGAGGAG
    AAAACAGACA ACGCCACCCT GCTGCGCCTG TTGGAGGAGG GCGAAAAGAT CCAGCACATG
    TACCGCTGTG CTCGAGTCCA GGGCCTTGAT ACCAGTGAGG GGCTACTCCT CTTTGGTAAA
    GAGCATTTCT ATGTGATTGA TGGATTTACA ATGACAGCAA CCAGAGAAAT AAGAGATATT
    GAAACCTTAC CTCCAAATAT GCATGAGCCG ATTATCCCTA GAGGAGCCAG GCAAGGCCCC
    AGTCAGCTCA AGAGAACGTG CAGTATCTTT GCATATGAAG ATATCAAGGA AGTGCATAAA
    CGGAGATATC TCCTGCAGCC TATCGCTGTG GAAGTTTTCT CTGGAGATGG ACGAAACTAC
    CTCCTTGCTT TTCAGAAAGG AATCAGAAAC AAAGTTTATC AAAGGTTTTT GGCTGTAGTG
    CCATCTCTCA CTGACAGTTC AGAGTCTGTG TCTGGGCAGC GGCCGAACAC CAGCGTGGAG
    CAGGGATCTG GGTTGCTTAG CACTCTGGTT GGAGAGAAGT CTGTGACTCA GAGATGGGAG
    AGAGGTGAAA TCAGCAACTT TCAGTATTTG ATGCACTTGA ACACTTTGGC TGGAAGATCG
    TATAATGACC TCATGCAATA TCCTGTCTTC CCCTGGATCC TTGCAGATTA CGACTCTGAG
    GAGGTCGATC TTACTAATCC CAAGACATTT AGAAACCTGG CCAAGCCAAT GGGAGCACAG
    ACAGATGAAC GATTAGCTCA GTATAAGAAG CGATATAAAG ACTGGGAAGA TCCTAATGGA
    GAAACTCCAG CCTACCACTA TGGGACCCAC TATTCATCAG CCATGATTGT GGCTTCATAC
    CTTGTCAGAA TGGAGCCCTT CACACAGATA TTCCTAAGGC TACAGGGAGG CCACTTTGAC
    CTGGCTGACC GGATGTTTCA CAGCGTGCGT GAGGCCTGGT ACTCAGCGTC AAAGCACAAC
    ATGGCAGATG TAAAGGAACT CATCCCAGAA TTTTTTTACT TACCAGAGTT CCTGTTCAAT
    TCCAACAACT TTGACCTTGG CTGTAAACAA AACGGCACCA AACTTGGAGA TGTTATCCTT
    CCACCCTGGG CAAAAGGGGA CCCACGAGAA TTCATCCGGG TGCACCGTGA GGCTCTGGAA
    TGTGACTACG TGAGTGCCCA TCTGCATGAG TGGATCGACT TAATCTTCGG CTATAAGCAG
    CAAGGTCCTG CCGCAGTAGA AGCTGTAAAC GTCTTCCATC ACCTTTTCTA TGAGGGTCAA
    GTGGATATCT ACAACATAAA TGACCCCCTC AAGGAAACAG CCACCATAGG GTTCATTAAT
    AACTTTGGGC AGATCCCTAA ACAGTTATTT AAAAAACCTC ATCCACCCAA GCGAGTGAGG
    AGTCGACTCA ACGGAGACAA TGCGGGGGCC TCCGTCCCGC CAGGGTCTGC CAGTGACAAG
    ATCTTTTTTC ATCATCTGGA CAACCTGAGG CCTTCACTAA CACCTGTAAA AGAACTCAAA
    GAGCCTGTGG GACAAATCGT GTGTACAGAC AAAGGCATTC TTGCGGTAGA ACAGAATAAG
    GTTCTTATCC CACCAACCTG GAACAAGACT TTCGCCTGGG GCTACGCAGA CCTCAGCTGC
    AGGCTGGGGA CCTATGAGTC AGACAAGGCA GTGACTGTTT ATGAATGTTT GTCGGAGTGG
    GGCCAGATTC TCTGTGCGAT CTGCCCCAAC CCCAAGCTGG TCATCACGGG TGGAACAAGT
    ACGGTGGTGT GTGTTTGGGA GATGAGTACC TCGAAAGAAA AGGCTAAGAC TCTCACTCTC
    AAACAGGCCT TACTTGGCCA CACGGATACG GTTACCTGTG CCACCGCGTC GTTAGCCTAT
    CACATCATCG TCAGCGGGTC CCGAGACCGA ACCTGCATCA TCTGGGACCT GAACAAGCTG
    TCATTCCTAA CCCAGCTCCG AGGCCACCGG GCTCCCGTGT CTGCTCTGTG TATCAATGAA
    TTAACCGGGG ACATCGTGTC CTGCGCCGGC ACGTACATCC ATGTGTGGAG CATAAACGGG
    AACCCCGTCG TGAGTGTGAA CACGTTCACA GGCAGGAGCC AGCAGATCGT CTGTTGCTGT
    GTGTCGGAGA TGAACGAGTG GGACACGCAG AACGTCATCG TCACGGGACA CTCGGATGGC
    GTGGTCCGGT TTTGGAGAAT GGAATTTTTG CAAGTTCCTG AAACACCAGC CCCTGAGCCT
    GCTGAAGTCC TCGAAATGCA GGAAGACTGT CCAGAAGCAC AAATCGGGCA AGAAGCCCAG
    GACGAGGACA GTAGCGATTC CGAGGCCGAC GAGCAGAGCG TCAGCCAGGA CCCCAAGGAC
    ACGCCCAGCC AGCCCAGCAG CACCAGCCAC CGGCCCCGGG CAGCCTCCTG CAGAGCCACA
    GCCACCTGGT ATAACGACAG TGGCTCCGAC GACTCCAGGC GCTGGTCCGA TCAGCTCAGC
    CTCGATGAGA AAGACGGCTT CATATTTGTG AACTATTCAG AGGGCCAGAG CAGACCTCAT
    CTTCAGACTC CCCTTAACCA CCCCCACCCC AATCCCATCG AGGCGCGGAA TTACAGCCGA
    TTGAAGCCTG GGTACCGCTG GGAACGGCAG CTCGTGTTCA GGAGTAAGCT GACCATGCAC
    ACGGCCTTTG ACCGGAAGGA CAATGCTCAC CCTGCCGAGA TCACTGCATT GGGCGTCTCC
    AAGGACCACA GCAGGATCCT CGTGGGCGAC AGTCGGGGCC GGGTGTTCAG CTGGTCTGTG
    AGCGACCAGC CCGGCCGCTC TGCCGCTGAC CACTGGGTGA AGGACGAAGG AGGGGACAGC
    TGTTCAGGCT GCTCCGTGCG GTTCTCTCTC ACGGAAAGAC GGCACCATTG CAGGAACTGT
    GGTCAGCTCT TCTGCCAGAA GTGCAGCCGA TTTCAGTCAG AAATCAAGCG CTTGAAAATC
    TCCTCCCCAG TACGTGTCTG TCAGAACTGT TACTACAACT TACAGCATGA GCGGAGTTCA
    GAAGACGGGC CTCGGAATTG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327189.2
    CDS267..10850
    Translation

    Target ORF information:

    RefSeq Version XM_015471703.2
    Organism Bos taurus(cattle)
    Definition Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471703.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    ATGAACATGG TGAAGCGGAT CATGGGGCGG CCGAGGCAGG AGGAATGCAG CCCGCAAGAT 
    AACGCCCTAG GACTGATGCA CCTCCGCAGG CTCTTCACAG AGCTGTGCCA CCCTCCTCGG
    CACATGACCC AGAAGGAACA AGAAGAGAAG CTGTACATGA TGCTGCCAGT GTTTAACAGG
    GTTTTTGGAA ACGCTCCGCC AAATACAATG ACAGAAAAAT TTTCTGATCT GCTGCAGTTC
    ACAACACAAG TCTCACGACT GATGGTGACA GAAATTCGAA GGAGAGCATC AAACAAATCC
    ACAGAGGCTG CAAGTCGGGC CATAGTCCAG TTCTTGGAAA TCAATCAGAG TGAAGAAGCC
    AGTAGAGGTT GGATGCTTCT GACAACAATT AATTTGTTAG CATCCTCTGG TCAGAAAACC
    GTGGACTGCA TGACAACAAT GTCAGTGCCT TCCACCCTGG TTAAATGTTT ATATCTGTTT
    TTTGACCTTC CACATGTGCC TGAGGCAGTT GGAGGTGCAC AGAATGAGCT ACCTCTAGCA
    GAACGTCGGG GACTACTCCA GAAAGTTTTT GTACAGATCT TAGTGAAACT CTGCAGTTTT
    GTTTCTCCGG CCGAGGAGCT GGCTCAGAAA GACGATCTCC AGCTTTTATT CAGTGCAATA
    ACCTCTTGGT GCCCTCCCTA TAACCTGCCT TGGAGGAAGA GTGCCGGAGA AGTCCTTATG
    ACCATATCCC GTCATGGTCT CAGTGTCAAC GTGGTGAAGT ATATTCACGA AAAAGAATGT
    TTATCTACGT GTGTTCAGAA TATGCAGCAG TCAGATGACC TGTCTCCCCT AGAAATTGTT
    GAGATGTTTG CTGGGCTTTC TTGTTTCCTC AAAGATTCCA GTGATGTTTC CCAAACTCTT
    CTGGATGATT TTCGGATATG GCAAGGATAT AATTTCCTTT GTGATCTCTT GCTTAGATTG
    GAACAAGCAA AAGAGGCAGA ATCCAAAGAT GCCTTGAAAG ATCTGGTTAA TCTGATAACT
    TCCCTAACAA CATATGGTGT CAATGAACTA AAACCAGCTG GTATTACCAC AGGGGCACCC
    TTTTTATTGC CTGGATTTGC AGTACCTCAG CCTGCGGGCA AAGGTCACAG TGTGAGAAAC
    ATCCAGGCCT TTGCAGTTCT TCAAAATGCA TTTTTAAAAG CAAAAACCAA CTTCCTCGCC
    CACATCATTC TCGATGCCAT CACAAATATT TACATGGCTG ACAACGCCAA CTATTTCATC
    CTCGAATCAC AGCACACACT GTCACAGTTT GCAGAAAAAA TTTCTAAACT CCCAGAAGTA
    CAAAACAAGT ACTTCGAGAT GCTGGAGTTT GTTGTTTTTA GCTTAAATTA TATCCCTTGT
    AAAGAACTTA TTAGTGTTAG CATCCTCTTA AAGTCTAGCT CTTCTTATCA CTGTAGCATT
    ATCGCGATGA AAACACTTCT TAAGTTTACA CGACATGACT ACATATTTAA AGATGTGTTC
    AGGGAGGTGG GCCTTTTGGA GGTCATGGTA AACCTTTTGC ACAAATATGC TGCCCTCTTG
    AAGGATCCAA CTCAGGCACT AAATGAACAA GGGGACTCAA GAAATAATAG TTCATTTGAA
    GACCAAAAAC ACCTGGCTTT GTTAGTCATG GAGACTTTGA CAGTGCTTCT TCAAGGGTCA
    AACACAAACG CAGGAATTTT CCGAGAATTT GGAGGCGCAA GATGTGCGCA TAATATAGTG
    AGGTACCCAC AGTGTCGGCA GCATGCCCTG ATGATGATCC AGCAGTTGGT GCTGTCTCCC
    AATGGGGACG ATGACATGGG CACGCTTCTG GGACTCATGC ACTCGGCCCC ACCCACAGAG
    CTGCAGCTGA AGACCGACAT CCTCAGAGCC CTCCTGTCGG TCCTTCGAGA AAGTCACCGT
    TCCAGAACAG TCTTTAGGAA GGTTGGAGGC TTTGTGTACG TTACGTCCTT GCTCGTGGCT
    ATGGAAAGGT CTTTGAGTGC GCCCCCCAAG AATGGGTGGG AGAAAGTGAG CCAGGGCCAC
    GTGTTTGAAC TTCTCCACAC CGTGTTCTGC ACACTGACTG CAGCGATGCG TTATGAGCCA
    GCCAACTCAC ACTTCTTCAA AACAGAGATT CAGTATGAGA AGCTGGCAGA CGCTGTTCGA
    TTTCTTGGCT GCTTCTCAGA CCTAAGAAAG ATCAGCCCCA TGAATGTCTT CCCTTCAAAT
    ACTCAGCCAT TTCAAAGACT TTTAGAGGAA GACGTCATCT CGATGGACTC GGTGTCACCC
    ACTCTGCGGC ACTGCAGTAA ACTCTTTATT TATCTCTACA GAGTAGCCAC AGATTCTTTT
    GACAGTCGTG CAGAACAGAT CCCTCCTTGC CTGACAAGTG AGTCTTCTCT TCCCTCTCCT
    TGGGGGACTC CAGCTTTGTC CAGGAAAAGG CACGCGTATC ATTCTGTTTC TACTCCCCCC
    GTCTACCCTC CTAAAAACGT TGCTGACCTG AAACCACACG TGGCAACTTC ATCTCTCTCA
    AGTTCTGATG CCGTCATCAT CCATCCTGGA GCCATGCTTG CTATGCTGGA TCTCCTGGCC
    TCTGTTGGGT CAGTGACACA GCCAGAACAT GCCTTGGACC TTCAACTTGC CGTGGCCAAT
    ATTTTACAAT CCCTGGTGCA CACGGAAAGA AACCAGCAGG TCATGTGTGA AGCTGGTCTC
    CATGCACGAC TGCTGCAGCG GTGCAGTGCC GCTCTGGCTG ACGAGGACCA CTCGCTGCAC
    CCACCCCTGC AGCGCATGTT CGAGCGATTA GCCTCTCAAG CGCTGGAGCC CATGGTGCTC
    AGGGAGTTTT TGCGTTTGGC AAGTCCATTA AATTGTGGTG CCTGGGACAA AAAACTGCTA
    AAACAATATA GGGTCCACAA ACCAAGTTCA CTGAGTTTTG AGCCAGAGAT GAGAAGCAGT
    ATGATCACAT CCATGGAAGG TCTGGGTTCT GATAATGTTT TCAGCTTACA TGAAGATAAC
    CATTACCGGA TAAGCAAGAG CCTAGTGAAA TCTGCAGAAG GAAGTACTGT ACCCCTAACC
    AGAGTGAAAT GTCTGGTCTC CATGACAACC CCACATGACA TCAGACTTCA TGGGTCATCA
    GTTACTCCAG CTTTTGTTGA ATTTGACACA TCCCTTGAAG GGTTTGGCTG CCTGTTCCTG
    CCCAGTCTGG CCCCTCATAA TGCCCCTACA AACAATGCTG TCACAACAGG TCTTATCGAT
    GGGGCCGTGG TCAGCGGTAT TGGTTCTGGT GAACGATTCT TCCCTCCTCC ATCTGGCTTA
    AGCTACTCCA GCTGGTTTTG CATTGAACAT TTTAGTTCTC CTCCCAGTAA CCACCCAGTC
    AGACTCCTTA CTGTTGTTCG CCGAGCAAAT TCTTCTGAGC ACCATTATGT ATGCCTTGCC
    ATAGTTCTCT CTGCAAAAGA TCGGTCTCTG ATTGTCTCCA CCAAAGAGGA ACTACTCCAA
    AATTATGTTG ATGATTTTAG TGAAGAATCC TCTTTCTATG AGATTCTGCC ATGCTGTGCT
    CGCTTCCGTT GTGGAGAACT GATCGTTGAG GGACAGTGGC ATCACCTGGT TCTGGTGATG
    AGCAAAGGGA TGCTGAAGAA CAGCACTGCA ACCCTCTACA TCGACGGCCA GCTTGTCAGC
    ACTGTGAAGC TTCATTATGT TCACAGTACT CCCGGGGGCT CAGGCTCTGC AAACCCACCA
    GTGGTTAGCA CAGTCTATGC CTACATTGGT ACTCCGCCTG CCCAGCGCCA AATTGCTTCA
    TTGGTTTGGC GCCTGGGACC TACACACTTT CTAGAAGAAG TATTACCTCC TTCAAATGTT
    ACTACCATTT ATGAACTAGG ACCAAATTAC GTGGGAAGCT TTCAGGCCGT ATGTATGCCA
    TGTAAAGATG CAAAATCTGA AGGTGTGGTC CCATCTCCTG TGTCATTAGT ACCAGAGGAA
    AAGGTGTCAT TCGGCCTCTA TGCACTGTCC GTGTCCGCCT TGACAGTGGC GAGAATCCGG
    AAGGTGTACA ACAAGCTGGA CAGCAAAGCC ATCGCTAAGC AGTTAGGCAT TTCCTCCCAT
    GAGAACGCCA CTCCTGTGAA GCTGATCCAC AACTCCGCAG GACACCTCAA TGGGCCCGCA
    CGGACAATCG GGGCCAGTCT GATTGGATAC TTGGGAGTAA GAACATTTGT TCCTAAGCCT
    GTTGCCACTA CTTTGCAGTA CGTTGGTGGG GCCGCAGCCA TCCTGGGCCT GGTAGCCATG
    GCTTCTGATG TGGAAGGGCT GTATGCAGCG GTCAAGGCCC TAGTTTGTGT GGTCAAGAGT
    AACCCACTAG CCAGCAAAGA AATGGAAAGA ATCCGGGGCT ACCAGTTGCT GGCAATGCTG
    TTTAAGAAGA AACGCTCCCT TCTTAACAGC CACATTCTCC ATCTCACTTT TTCCTTGGTG
    GGAACTGTTG ACAGTGGACA TGAGACTTCC ATTATTCCAA ATTCAACAGC TTTCCAGGAT
    CTACTTTGTG ATTTTGAAGT CTGGCTCCAT GCTCCATATG AACTTCATCT CTCCCTATTT
    GAACACTTTA TTGAATTGCT CACAGAGTCC AGTGAAGCTT CAAAGAATGC CAAGTTGATG
    AGGGAATTCC AGCTAATCCC AAAGCTGCTA CTGACTCTTC GAGATATGTC TCTATCCCAG
    CCTACCATTG CTGCTATTAG TAACGTGCTG AGCTTCTTAC TGCAGGGTTT TCCCAACAGC
    AATGATCTAC TCAGGTTTGG CCAGTTCATT TCTTCTACTT TACCAACCTT TGCAGTTTGT
    GAGAAGTTTG TAGTTATGGA AATAAACCAC GAAGAGAAGC CTGACACTGG AACTGAAGAA
    GAGTTTGGAG GTCTTGTATC TGCTAATCTT ATACTTTTGA GGAACAGACT TCTGGATATC
    TTACTAAAAC TGGTTTACAC ATCTAAAGAA AAGACAAGCA TTAATTTGCA AGCTTGTGAA
    GAGCTGGTCA GGACGCTGGG CTTTGACTGG ATTATGATGT TTATGGAAGA ACACTTGCAT
    TCTACCACAG TCACAGCAGC CATGAGGATT CTTGTTGTCC TACTGAGTAA TCAGTCTATT
    CTCATCAAGT TCAAGGAGGG ACTCAGTGGT GGAGGCTGGC TGGAGCAGAC AGATTCTGTC
    TTAACGAATA AGATCGGAAC TGTATTAGGA TTCAATGTGG GCAGGAGTGC TGGCGGACGA
    TCAACTGTCA GGGAGATTAA CCGGGATGCT TGTCACTTTC CTGGCTTTCC GGTGCTTCAG
    TCATTCCTTC CTAAACACAC TAATGTCCCT GCTCTCTATT TCCTCCTCAT GGCATTGTTT
    CTGCAGCAGC CCGTTAGTGA GCTGCCTGAG AACCTGCAGG TCAGTGTGCC TGTCGTCAGC
    AGCCGATGTA AGCAGGGTTG CCAGTTTGAT TTAGATTCCA TCTGGACCTT TATCTTTGGA
    GTTCCTGCCT CCAGTGGAAC CGTGGTCTCA TCTATCCACA ATGTGTGCAC AGAAGCTGCT
    TTTTTGTTAC TGGGCATGCT CCGAAGCATG CTGAATTCAC CTTGGCAATC AGAAGAAGAG
    GGGTCTTGGC TCCGGGAGTA TCCTGTGACC CTGATGCAGT TCTTCAGATA CTTGTATCAC
    AATGTCCCGG ACCTGGCCTC CATGTGGATG AACCCTGATT TCCTGTGTGC ACTTGCAGCC
    ACGGTCTTCC CCTTCAACAT CCGCCCTTAT TCAGAAATGG TGACTGATCT CGATGATGAA
    GTTGGATCTC CAGCTGAAGA ATTTAAAGCC TTTGCAGCAG ACACAGGGAT GAACAGAAGC
    CAATCAGAGT ACTGCAACGT GGGCGTCAAG ACATACCTGA CTAATCACCC AGCTAAGAAA
    TTTGTTTTTG ACTTCATGCG GGTCTTAATA ATAGACAACC TCTGTCTTAC ACCTGCCAGC
    AAGCAGACTC CACTGATTGA TCTTTTGTTG GAGGCTTCTC CTGAGAGATC GACAAGAACG
    CAGCAAAAAG AATTTCAGAC TTACATTTTG GATAGTGTGA TGGATCATTT GCTTGCAGCT
    GATGTTTTAC TGGGAGAAGA TGCATCTCTT CCTATCACCA GTGGAGGAAG CTACCAGGTA
    TTGGTGAACA ATGTGTTTTA TTTCACACAA CGTGTGGTGG ACAAGCTTTG GCAAGGCATG
    TTCAACAAAG AATCTAAACT TCTTATAGAT TTTATAATTC AACTAATTGC ACAGTCAAAG
    AGAAGATCCC AGGGGTTGTC ACTGGATGCA GTCTATCACT GCCTCAACAG GACTATCCTG
    TACCAGTTCT CCCGGGCGCA CAAAACCGTC CCGCAGCAAG TAGCCCTTCT TGACTCTCTC
    AGGGTCCTGA CTGTCAACAG GAACTTGATC CTGGGCCCCG GAAACCACGA CCAAGAATTC
    ATCAGCTGTC TGGCTCACTG CCTCATTAAC CTGCATGTTG GGAGCAATGT GGATGGATTT
    GGACTGGAAG CAGAAGCCCG CATGACTACA TGGCATATTA TGATCCCCTC GGACATTGAA
    CCAGATGGTG GTTACAGCCA AGATATTAGT GAAGGGCGTC AGCTTCTCAT AAAAGCTGTC
    AACAGAGTTT GGACGGAATT AATTCATAGT AAGAAACAAG TCTTGGAGGA GCTTTTCAAA
    GTCACTCTAC CTGTGAATGA AAGAGGCCAC GTGGACATAG CTATAGCAAG GCCGCTCATC
    GAAGAAGCTA GTTTGAAGTG CTGGCAGAAT CATCTAGCCC ATGAAAAGAA GTGCATAAGT
    CGAGGAGAAG CTTTAATGCC CACCACACAA TCCAAATTGT CCCGTGTCAG CAGTGGCTTT
    GGTCTTTCCA AGTTAACGGG ATCAAGAAGG AATCGAAAGG AAAGCGGGCT TCATAAACAC
    AGCCTTTCCA CCCAGGAGAT TTCTCAGTGG ATGTTTACAC ACATCGCTGT TGTTCGTGAC
    TTGGTAGACA CACAATATAA GGAATACCAG GAGCGTCAGC AAAATGCCCT GAAGTACGTG
    ACGGAAGAGT GGTGCCAGAT CGAGTATGAG CTGCTGCGGG AGCGGGGACT CTGGGGCCCC
    CCCATTGGTT CCCATCTCGA CAAGTGGATG TTGGAGATGA CAGAGGGTCC CTGCAGAATG
    AGGAAAAAAA TGGTGCGAAA TGATATGTTT TATAACCATT ACCCTTACGT GCCAGAAATG
    GAGCAGGAAA CCAACGTGGC GTCTGAGATC CCAAGTAAAC AGCCTGAGAC ACCTGATGAT
    ATCATTCCTC AAAAGAAGCC TGCTCGATAC AGAAGAGCCG TAAGTTATGA CAGTAAAGAA
    TACTACATGC GACTAGCCTC CGGCAACCCA GCCATTGTGC AGGATGCCAT CGTAGAGAGC
    TCTGAAGGAG AAGCTGCTCA GCAAGAACCA GAACATGGTG AAGACACGAT TGCTAAAGTC
    AAAGGACTGG TCAAGCCACC TCTCAAACGG TCTCGGTCAG CACCGGACGG AGGCGATGAG
    GAGACACAGG AGCAGTTACA GGACCAGACA GCTGAGGGCA GCTCGATAGA AGAGGAGGAG
    AAAACAGACA ACGCCACCCT GCTGCGCCTG TTGGAGGAGG GCGAAAAGAT CCAGCACATG
    TACCGCTGTG CTCGAGTCCA GGGCCTTGAT ACCAGTGAGG GGCTACTCCT CTTTGGTAAA
    GAGCATTTCT ATGTGATTGA TGGATTTACA ATGACAGCAA CCAGAGAAAT AAGAGATATT
    GAAACCTTAC CTCCAAATAT GCATGAGCCG ATTATCCCTA GAGGAGCCAG GCAAGGCCCC
    AGTCAGCTCA AGAGAACGTG CAGTATCTTT GCATATGAAG ATATCAAGGA AGTGCATAAA
    CGGAGATATC TCCTGCAGCC TATCGCTGTG GAAGTTTTCT CTGGAGATGG ACGAAACTAC
    CTCCTTGCTT TTCAGAAAGG AATCAGAAAC AAAGTTTATC AAAGGTTTTT GGCTGTAGTG
    CCATCTCTCA CTGACAGTTC AGAGTCTGTG TCTGGGCAGC GGCCGAACAC CAGCGTGGAG
    CAGGGATCTG GGTTGCTTAG CACTCTGGTT GGAGAGAAGT CTGTGACTCA GAGATGGGAG
    AGAGGTGAAA TCAGCAACTT TCAGTATTTG ATGCACTTGA ACACTTTGGC TGGAAGATCG
    TATAATGACC TCATGCAATA TCCTGTCTTC CCCTGGATCC TTGCAGATTA CGACTCTGAG
    GAGGTCGATC TTACTAATCC CAAGACATTT AGAAACCTGG CCAAGCCAAT GGGAGCACAG
    ACAGATGAAC GATTAGCTCA GTATAAGAAG CGATATAAAG ACTGGGAAGA TCCTAATGGA
    GAAACTCCAG CCTACCACTA TGGGACCCAC TATTCATCAG CCATGATTGT GGCTTCATAC
    CTTGTCAGAA TGGAGCCCTT CACACAGATA TTCCTAAGGC TACAGGGAGG CCACTTTGAC
    CTGGCTGACC GGATGTTTCA CAGCGTGCGT GAGGCCTGGT ACTCAGCGTC AAAGCACAAC
    ATGGCAGATG TAAAGGAACT CATCCCAGAA TTTTTTTACT TACCAGAGTT CCTGTTCAAT
    TCCAACAACT TTGACCTTGG CTGTAAACAA AACGGCACCA AACTTGGAGA TGTTATCCTT
    CCACCCTGGG CAAAAGGGGA CCCACGAGAA TTCATCCGGG TGCACCGTGA GGCTCTGGAA
    TGTGACTACG TGAGTGCCCA TCTGCATGAG TGGATCGACT TAATCTTCGG CTATAAGCAG
    CAAGGTCCTG CCGCAGTAGA AGCTGTAAAC GTCTTCCATC ACCTTTTCTA TGAGGGTCAA
    GTGGATATCT ACAACATAAA TGACCCCCTC AAGGAAACAG CCACCATAGG GTTCATTAAT
    AACTTTGGGC AGATCCCTAA ACAGTTATTT AAAAAACCTC ATCCACCCAA GCGAGTGAGG
    AGTCGACTCA ACGGAGACAA TGCGGGGGCC TCCGTCCCGC CAGGGTCTGC CAGTGACAAG
    ATCTTTTTTC ATCATCTGGA CAACCTGAGG CCTTCACTAA CACCTGTAAA AGAACTCAAA
    GAGCCTGTGG GACAAATCGT GTGTACAGAC AAAGGCATTC TTGCGGTAGA ACAGAATAAG
    GTTCTTATCC CACCAACCTG GAACAAGACT TTCGCCTGGG GCTACGCAGA CCTCAGCTGC
    AGGCTGGGGA CCTATGAGTC AGACAAGGCA GTGACTGTTT ATGAATGTTT GTCGGAGTGG
    GGCCAGATTC TCTGTGCGAT CTGCCCCAAC CCCAAGCTGG TCATCACGGG TGGAACAAGT
    ACGGTGGTGT GTGTTTGGGA GATGAGTACC TCGAAAGAAA AGGCTAAGAC TCTCACTCTC
    AAACAGGCCT TACTTGGCCA CACGGATACG GTTACCTGTG CCACCGCGTC GTTAGCCTAT
    CACATCATCG TCAGCGGGTC CCGAGACCGA ACCTGCATCA TCTGGGACCT GAACAAGCTG
    TCATTCCTAA CCCAGCTCCG AGGCCACCGG GCTCCCGTGT CTGCTCTGTG TATCAATGAA
    TTAACCGGGG ACATCGTGTC CTGCGCCGGC ACGTACATCC ATGTGTGGAG CATAAACGGG
    AACCCCGTCG TGAGTGTGAA CACGTTCACA GGCAGGAGCC AGCAGATCGT CTGTTGCTGT
    GTGTCGGAGA TGAACGAGTG GGACACGCAG AACGTCATCG TCACGGGACA CTCGGATGGC
    GTGGTCCGGT TTTGGAGAAT GGAATTTTTG CAAGTTCCTG AAACACCAGC CCCTGAGCCT
    GCTGAAGTCC TCGAAATGCA GGAAGACTGT CCAGAAGCAC AAATCGGGCA AGAAGCCCAG
    GACGAGGACA GTAGCGATTC CGAGGCCGAC GAGCAGAGCG TCAGCCAGGA CCCCAAGGAC
    ACGCCCAGCC AGCCCAGCAG CACCAGCCAC CGGCCCCGGG CAGCCTCCTG CAGAGCCACA
    GCCACCTGGT ATAACGACAG TGGCTCCGAC GACTCCAGGC GCTGGTCCGA TCAGCTCAGC
    CTCGATGAGA AAGACGGCTT CATATTTGTG AACTATTCAG AGGGCCAGAG CAGACCTCAT
    CTTCAGACTC CCCTTAACCA CCCCCACCCC AATCCCATCG AGGCGCGGAA TTACAGCCGA
    TTGAAGCCTG GGTACCGCTG GGAACGGCAG CTCGTGTTCA GGAGTAAGCT GACCATGCAC
    ACGGCCTTTG ACCGGAAGGA CAATGCTCAC CCTGCCGAGA TCACTGCATT GGGCGTCTCC
    AAGGACCACA GCAGGATCCT CGTGGGCGAC AGTCGGGGCC GGGTGTTCAG CTGGTCTGTG
    AGCGACCAGC CCGGCCGCTC TGCCGCTGAC CACTGGGTGA AGGACGAAGG AGGGGACAGC
    TGTTCAGGCT GCTCCGTGCG GTTCTCTCTC ACGGAAAGAC GGCACCATTG CAGGAACTGT
    GGTCAGCTCT TCTGCCAGAA GTGCAGCCGA TTTCAGTCAG AAATCAAGCG CTTGAAAATC
    TCCTCCCCAG TACGTGTCTG TCAGAACTGT TACTACAACT TACAGCATGA GCGGAGTTCA
    GAAGACGGGC CTCGGAATTG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90115
    Clone ID Related Accession (Same CDS sequence) XM_010797980.2 , XM_015471704.1 , XM_015463824.1 , XM_015471703.1
    Accession Version XM_010797980.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WD repeat and FYVE domain-containing protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644958.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010797980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGTC GTGCAGAACA GATCCCTCCT TGCCTGACAA GTGAGTCTTC TCTTCCCTCT
    CCTTGGGGGA CTCCAGCTTT GTCCAGGAAA AGGCACGCGT ATCATTCTGT TTCTACTCCC
    CCCGTCTACC CTCCTAAAAA CGTTGCTGAC CTGAAACCAC ACGTGGCAAC TTCATCTCTC
    TCAAGTTCTG ATGCCGTCAT CATCCATCCT GGAGCCATGC TTGCTATGCT GGATCTCCTG
    GCCTCTGTTG GGTCAGTGAC ACAGCCAGAA CATGCCTTGG ACCTTCAACT TGCCGTGGCC
    AATATTTTAC AATCCCTGGT GCACACGGAA AGAAACCAGC AGGTCATGTG TGAAGCTGGT
    CTCCATGCAC GACTGCTGCA GCGGTGCAGT GCCGCTCTGG CTGACGAGGA CCACTCGCTG
    CACCCACCCC TGCAGCGCAT GTTCGAGCGA TTAGCCTCTC AAGCGCTGGA GCCCATGGTG
    CTCAGGGAGT TTTTGCGTTT GGCAAGTCCA TTAAATTGTG GTGCCTGGGA CAAAAAACTG
    CTAAAACAAT ATAGGGTCCA CAAACCAAGT TCACTGAGTT TTGAGCCAGA GATGAGAAGC
    AGTATGATCA CATCCATGGA AGGTCTGGGT TCTGATAATG TTTTCAGCTT ACATGAAGAT
    AACCATTACC GGATAAGCAA GAGCCTAGTG AAATCTGCAG AAGGAAGTAC TGTACCCCTA
    ACCAGAGTGA AATGTCTGGT CTCCATGACA ACCCCACATG ACATCAGACT TCATGGGTCA
    TCAGTTACTC CAGCTTTTGT TGAATTTGAC ACATCCCTTG AAGGGTTTGG CTGCCTGTTC
    CTGCCCAGTC TGGCCCCTCA TAATGCCCCT ACAAACAATG CTGTCACAAC AGGTCTTATC
    GATGGGGCCG TGGTCAGCGG TATTGGTTCT GGTGAACGAT TCTTCCCTCC TCCATCTGGC
    TTAAGCTACT CCAGCTGGTT TTGCATTGAA CATTTTAGTT CTCCTCCCAG TAACCACCCA
    GTCAGACTCC TTACTGTTGT TCGCCGAGCA AATTCTTCTG AGCACCATTA TGTATGCCTT
    GCCATAGTTC TCTCTGCAAA AGATCGGTCT CTGATTGTCT CCACCAAAGA GGAACTACTC
    CAAAATTATG TTGATGATTT TAGTGAAGAA TCCTCTTTCT ATGAGATTCT GCCATGCTGT
    GCTCGCTTCC GTTGTGGAGA ACTGATCGTT GAGGGACAGT GGCATCACCT GGTTCTGGTG
    ATGAGCAAAG GGATGCTGAA GAACAGCACT GCAACCCTCT ACATCGACGG CCAGCTTGTC
    AGCACTGTGA AGCTTCATTA TGTTCACAGT ACTCCCGGGG GCTCAGGCTC TGCAAACCCA
    CCAGTGGTTA GCACAGTCTA TGCCTACATT GGTACTCCGC CTGCCCAGCG CCAAATTGCT
    TCATTGGTTT GGCGCCTGGG ACCTACACAC TTTCTAGAAG AAGTATTACC TCCTTCAAAT
    GTTACTACCA TTTATGAACT AGGACCAAAT TACGTGGGAA GCTTTCAGGC CGTATGTATG
    CCATGTAAAG ATGCAAAATC TGAAGGTGTG GTCCCATCTC CTGTGTCATT AGTACCAGAG
    GAAAAGGTGT CATTCGGCCT CTATGCACTG TCCGTGTCCG CCTTGACAGT GGCGAGAATC
    CGGAAGGTGT ACAACAAGCT GGACAGCAAA GCCATCGCTA AGCAGTTAGG CATTTCCTCC
    CATGAGAACG CCACTCCTGT GAAGCTGATC CACAACTCCG CAGGACACCT CAATGGGCCC
    GCACGGACAA TCGGGGCCAG TCTGATTGGA TACTTGGGAG TAAGAACATT TGTTCCTAAG
    CCTGTTGCCA CTACTTTGCA GTACGTTGGT GGGGCCGCAG CCATCCTGGG CCTGGTAGCC
    ATGGCTTCTG ATGTGGAAGG GCTGTATGCA GCGGTCAAGG CCCTAGTTTG TGTGGTCAAG
    AGTAACCCAC TAGCCAGCAA AGAAATGGAA AGAATCCGGG GCTACCAGTT GCTGGCAATG
    CTGTTTAAGA AGAAACGCTC CCTTCTTAAC AGCCACATTC TCCATCTCAC TTTTTCCTTG
    GTGGGAACTG TTGACAGTGG ACATGAGACT TCCATTATTC CAAATTCAAC AGCTTTCCAG
    GATCTACTTT GTGATTTTGA AGTCTGGCTC CATGCTCCAT ATGAACTTCA TCTCTCCCTA
    TTTGAACACT TTATTGAATT GCTCACAGAG TCCAGTGAAG CTTCAAAGAA TGCCAAGTTG
    ATGAGGGAAT TCCAGCTAAT CCCAAAGCTG CTACTGACTC TTCGAGATAT GTCTCTATCC
    CAGCCTACCA TTGCTGCTAT TAGTAACGTG CTGAGCTTCT TACTGCAGGG TTTTCCCAAC
    AGCAATGATC TACTCAGGTT TGGCCAGTTC ATTTCTTCTA CTTTACCAAC CTTTGCAGTT
    TGTGAGAAGT TTGTAGTTAT GGAAATAAAC CACGAAGAGA AGCCTGACAC TGGAACTGAA
    GAAGAGTTTG GAGGTCTTGT ATCTGCTAAT CTTATACTTT TGAGGAACAG ACTTCTGGAT
    ATCTTACTAA AACTGGTTTA CACATCTAAA GAAAAGACAA GCATTAATTT GCAAGCTTGT
    GAAGAGCTGG TCAGGACGCT GGGCTTTGAC TGGATTATGA TGTTTATGGA AGAACACTTG
    CATTCTACCA CAGTCACAGC AGCCATGAGG ATTCTTGTTG TCCTACTGAG TAATCAGTCT
    ATTCTCATCA AGTTCAAGGA GGGACTCAGT GGTGGAGGCT GGCTGGAGCA GACAGATTCT
    GTCTTAACGA ATAAGATCGG AACTGTATTA GGATTCAATG TGGGCAGGAG TGCTGGCGGA
    CGATCAACTG TCAGGGAGAT TAACCGGGAT GCTTGTCACT TTCCTGGCTT TCCGGTGCTT
    CAGTCATTCC TTCCTAAACA CACTAATGTC CCTGCTCTCT ATTTCCTCCT CATGGCATTG
    TTTCTGCAGC AGCCCGTTAG TGAGCTGCCT GAGAACCTGC AGGTCAGTGT GCCTGTCGTC
    AGCAGCCGAT GTAAGCAGGG TTGCCAGTTT GATTTAGATT CCATCTGGAC CTTTATCTTT
    GGAGTTCCTG CCTCCAGTGG AACCGTGGTC TCATCTATCC ACAATGTGTG CACAGAAGCT
    GCTTTTTTGT TACTGGGCAT GCTCCGAAGC ATGCTGAATT CACCTTGGCA ATCAGAAGAA
    GAGGGGTCTT GGCTCCGGGA GTATCCTGTG ACCCTGATGC AGTTCTTCAG ATACTTGTAT
    CACAATGTCC CGGACCTGGC CTCCATGTGG ATGAACCCTG ATTTCCTGTG TGCACTTGCA
    GCCACGGTCT TCCCCTTCAA CATCCGCCCT TATTCAGAAA TGGTGACTGA TCTCGATGAT
    GAAGTTGGAT CTCCAGCTGA AGAATTTAAA GCCTTTGCAG CAGACACAGG GATGAACAGA
    AGCCAATCAG AGTACTGCAA CGTGGGCGTC AAGACATACC TGACTAATCA CCCAGCTAAG
    AAATTTGTTT TTGACTTCAT GCGGGTCTTA ATAATAGACA ACCTCTGTCT TACACCTGCC
    AGCAAGCAGA CTCCACTGAT TGATCTTTTG TTGGAGGCTT CTCCTGAGAG ATCGACAAGA
    ACGCAGCAAA AAGAATTTCA GACTTACATT TTGGATAGTG TGATGGATCA TTTGCTTGCA
    GCTGATGTTT TACTGGGAGA AGATGCATCT CTTCCTATCA CCAGTGGAGG AAGCTACCAG
    GTATTGGTGA ACAATGTGTT TTATTTCACA CAACGTGTGG TGGACAAGCT TTGGCAAGGC
    ATGTTCAACA AAGAATCTAA ACTTCTTATA GATTTTATAA TTCAACTAAT TGCACAGTCA
    AAGAGAAGAT CCCAGGGGTT GTCACTGGAT GCAGTCTATC ACTGCCTCAA CAGGACTATC
    CTGTACCAGT TCTCCCGGGC GCACAAAACC GTCCCGCAGC AAGTAGCCCT TCTTGACTCT
    CTCAGGGTCC TGACTGTCAA CAGGAACTTG ATCCTGGGCC CCGGAAACCA CGACCAAGAA
    TTCATCAGCT GTCTGGCTCA CTGCCTCATT AACCTGCATG TTGGGAGCAA TGTGGATGGA
    TTTGGACTGG AAGCAGAAGC CCGCATGACT ACATGGCATA TTATGATCCC CTCGGACATT
    GAACCAGATG GTGGTTACAG CCAAGATATT AGTGAAGGGC GTCAGCTTCT CATAAAAGCT
    GTCAACAGAG TTTGGACGGA ATTAATTCAT AGTAAGAAAC AAGTCTTGGA GGAGCTTTTC
    AAAGTCACTC TACCTGTGAA TGAAAGAGGC CACGTGGACA TAGCTATAGC AAGGCCGCTC
    ATCGAAGAAG CTAGTTTGAA GTGCTGGCAG AATCATCTAG CCCATGAAAA GAAGTGCATA
    AGTCGAGGAG AAGCTTTAAT GCCCACCACA CAATCCAAAT TGTCCCGTGT CAGCAGTGGC
    TTTGGTCTTT CCAAGTTAAC GGGATCAAGA AGGAATCGAA AGGAAAGCGG GCTTCATAAA
    CACAGCCTTT CCACCCAGGA GATTTCTCAG TGGATGTTTA CACACATCGC TGTTGTTCGT
    GACTTGGTAG ACACACAATA TAAGGAATAC CAGGAGCGTC AGCAAAATGC CCTGAAGTAC
    GTGACGGAAG AGTGGTGCCA GATCGAGTAT GAGCTGCTGC GGGAGCGGGG ACTCTGGGGC
    CCCCCCATTG GTTCCCATCT CGACAAGTGG ATGTTGGAGA TGACAGAGGG TCCCTGCAGA
    ATGAGGAAAA AAATGGTGCG AAATGATATG TTTTATAACC ATTACCCTTA CGTGCCAGAA
    ACGGAGCAGG AAACCAACGT GGCGTCTGAG ATCCCAAGTA AACAGCCTGA GACACCTGAT
    GATATCATTC CTCAAAAGAA GCCTGCTCGA TACAGAAGAG CCGTAAGTTA TGACAGTAAA
    GAATACTACA TGCGACTAGC CTCCGGCAAC CCAGCCATTG TGCAGGATGC CATCGTAGAG
    AGCTCTGAAG GAGAAGCTGC TCAGCAAGAA CCAGAACATG GTGAAGACAC GATTGCTAAA
    GTCAAAGGAC TGGTCAAGCC ACCTCTCAAA CGGTCTCGGT CAGCACCGGA CGGAGGCGAT
    GAGGAGACAC AGGAGCAGTT ACAGGACCAG ACAGCTGAGG GCAGCTCGAT AGAAGAGGAG
    GAGAAAACAG ACAACGCCAC CCTGCTGCGC CTGTTGGAGG AGGGCGAAAA GATCCAGCAC
    ATGTACCGCT GTGCTCGAGT CCAGGGCCTT GATACCAGTG AGGGGCTACT CCTCTTTGGT
    AAAGAGCATT TCTATGTGAT TGATGGATTT ACAATGACAG CAACCAGAGA AATAAGAGAT
    ATTGAAACCT TACCTCCAAA TATGCATGAG CCGATTATCC CTAGAGGAGC CAGGCAAGGC
    CCCAGTCAGC TCAAGAGAAC GTGCAGTATC TTTGCATATG AAGATATCAA GGAAGTGCAT
    AAACGGAGAT ATCTCCTGCA GCCTATCGCT GTGGAAGTTT TCTCTGGAGA TGGACGAAAC
    TACCTCCTTG CTTTTCAGAA AGGAATCAGA AACAAAGTTT ATCAAAGGTT TTTGGCTGTA
    GTGCCATCTC TCACTGACAG TTCAGAGTCT GTGTCTGGGC AGCGGCCGAA CACCAGCGTG
    GAGCAGGGAT CTGGGTTGCT TAGCACTCTG GTTGGAGAGA AGTCTGTGAC TCAGAGATGG
    GAGAGAGGTG AAATCAGCAA CTTTCAGTAT TTGATGCACT TGAACACTTT GGCTGGAAGA
    TCGTATAATG ACCTCATGCA ATATCCTGTC TTCCCCTGGA TCCTTGCAGA TTACGACTCG
    GAGGAGGTCG ATCTTACTAA TCCCAAGACA TTTAGAAACC TGGCCAAGCC AATGGGAGCA
    CAGACAGATG AACGATTAGC TCAGTATAAG AAGCGATATA AAGACTGGGA AGATCCTAAT
    GGAGAAACTC CAGCCTACCA CTATGGGACC CACTATTCAT CAGCCATGAT TGTGGCTTCA
    TACCTTGTCA GAATGGAGCC CTTCACACAG ATATTCCTAA GGCTACAGGG AGGCCACTTT
    GACCTGGCTG ACCGGATGTT TCACAGCGTG CGTGAGGCCT GGTACTCAGC GTCAAAGCAC
    AACATGGCAG ATGTAAAGGA ACTCATCCCA GAATTTTTTT ACTTACCAGA GTTCCTGTTC
    AATTCCAACA ACTTTGACCT TGGCTGTAAA CAAAACGGCA CCAAACTTGG AGATGTTATC
    CTTCCACCCT GGGCAAAAGG GGACCCACGA GAATTCATCC GGGTGCACCG TGAGGCTCTG
    GAATGTGACT ACGTGAGTGC CCATCTGCAT GAGTGGATCG ACTTAATCTT CGGCTATAAG
    CAGCAAGGTC CTGCCGCAGT AGAAGCTGTA AACGTCTTCC ATCACCTTTT CTATGAGGGT
    CAAGTGGATA TCTACAACAT AAATGACCCC CTCAAGGAAA CAGCCACCAT AGGGTTCATT
    AATAACTTTG GGCAGATCCC TAAACAGTTA TTTAAAAAAC CTCATCCACC CAAGCGAGTG
    AGGAGTCGAC TCAACGGAGA CAATGCGGGG GCCTCCGTCC CGCCAGGGTC TGCCAGTGAC
    AAGATCTTTT TTCATCATCT GGACAACCTG AGGCCTTCAC TAACACCTGT AAAAGAACTC
    AAAGAGCCTG TGGGACAAAT CGTGTGTACA GACAAAGGCA TTCTTGCGGT AGAACAGAAT
    AAGGTTCTTA TCCCACCAAC CTGGAACAAG ACTTTCGCCT GGGGCTACGC AGACCTCAGC
    TGCAGGCTGG GGACCTATGA GTCAGACAAG GCAGTGACTG TTTATGAATG TTTGTCGGAG
    TGGGGCCAGA TTCTCTGTGC GATCTGCCCC AACCCCAAGC TGGTCATCAC GGGTGGAACA
    AGTACGGTGG TGTGTGTTTG GGAGATGAGT ACCTCGAAAG AAAAGGCTAA GACTCTCACT
    CTCAAACAGG CCTTACTTGG CCACACGGAT ACGGTTACCT GTGCCACCGC GTCGTTAGCC
    TATCACATCA TCGTCAGCGG GTCCCGAGAC CGAACCTGCA TCATCTGGGA CCTGAACAAG
    CTGTCATTCC TAACCCAGCT CCGAGGCCAC CGGGCTCCCG TGTCTGCTCT GTGTATCAAT
    GAATTAACCG GGGACATCGT GTCCTGCGCC GGCACGTACA TCCATGTGTG GAGCATAAAC
    GGGAACCCCG TCGTGAGTGT GAACACGTTC ACAGGCAGGA GCCAGCAGAT CGTCTGTTGC
    TGTGTGTCGG AGATGAACGA GTGGGACACG CAGAACGTCA TCGTCACGGG ACACTCGGAT
    GGCGTGGTCC GGTTTTGGAG AATGGAATTT TTGCAAGTTC CTGAAACACC AGCCCCTGAG
    CCTGCTGAAG TCCTCGAAAT GCAGGAAGAC TGTCCAGAAG CACAAATCGG GCAAGAAGCC
    CAGGACGAGG ACAGTAGCGA TTCCGAGGCC GACGAGCAGA GCGTCAGCCA GGACCCCAAG
    GACACGCCCA GCCAGCCCAG CAGCACCAGC CACCGGCCCC GGGCAGCCTC CTGCAGAGCC
    ACAGCCACCT GGTATAACGA CAGTGGCTCC GACGACTCCA GGCGCTGGTC CGATCAGCTC
    AGCCTCGATG AGAAAGACGG CTTCATATTT GTGAACTATT CAGAGGGCCA GAGCAGACCT
    CATCTTCAGA CTCCCCTTAA CCACCCCCAC CCCAATCCCA TCGAGGCGCG GAATTACAGC
    CGATTGAAGC CTGGGTACCG CTGGGAACGG CAGCTCGTGT TCAGGAGTAA GCTGACCATG
    CACACGGCCT TTGACCGGAA GGACAATGCT CACCCTGCCG AGATCACTGC ATTGGGCGTC
    TCCAAGGACC ACAGCAGGAT CCTCGTGGGC GACAGTCGGG GCCGGGTGTT CAGCTGGTCT
    GTGAGCGACC AGCCCGGCCG CTCTGCCGCT GACCACTGGG TGAAGGACGA AGGAGGGGAC
    AGCTGTTCAG GCTGCTCCGT GCGGTTCTCT CTCACGGAAA GACGGCACCA TTGCAGGAAC
    TGTGGTCAGC TCTTCTGCCA GAAGTGCAGC CGATTTCAGT CAGAAATCAA GCGCTTGAAA
    ATCTCCTCCC CAGTACGTGT CTGTCAGAAC TGTTACTACA ACTTACAGCA TGAGCGGAGT
    TCAGAAGACG GGCCTCGGAA TTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796282.2
    CDS998..10864
    Translation

    Target ORF information:

    RefSeq Version XM_010797980.2
    Organism Bos taurus(cattle)
    Definition Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010797980.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGTC GTGCAGAACA GATCCCTCCT TGCCTGACAA GTGAGTCTTC TCTTCCCTCT
    CCTTGGGGGA CTCCAGCTTT GTCCAGGAAA AGGCACGCGT ATCATTCTGT TTCTACTCCC
    CCCGTCTACC CTCCTAAAAA CGTTGCTGAC CTGAAACCAC ACGTGGCAAC TTCATCTCTC
    TCAAGTTCTG ATGCCGTCAT CATCCATCCT GGAGCCATGC TTGCTATGCT GGATCTCCTG
    GCCTCTGTTG GGTCAGTGAC ACAGCCAGAA CATGCCTTGG ACCTTCAACT TGCCGTGGCC
    AATATTTTAC AATCCCTGGT GCACACGGAA AGAAACCAGC AGGTCATGTG TGAAGCTGGT
    CTCCATGCAC GACTGCTGCA GCGGTGCAGT GCCGCTCTGG CTGACGAGGA CCACTCGCTG
    CACCCACCCC TGCAGCGCAT GTTCGAGCGA TTAGCCTCTC AAGCGCTGGA GCCCATGGTG
    CTCAGGGAGT TTTTGCGTTT GGCAAGTCCA TTAAATTGTG GTGCCTGGGA CAAAAAACTG
    CTAAAACAAT ATAGGGTCCA CAAACCAAGT TCACTGAGTT TTGAGCCAGA GATGAGAAGC
    AGTATGATCA CATCCATGGA AGGTCTGGGT TCTGATAATG TTTTCAGCTT ACATGAAGAT
    AACCATTACC GGATAAGCAA GAGCCTAGTG AAATCTGCAG AAGGAAGTAC TGTACCCCTA
    ACCAGAGTGA AATGTCTGGT CTCCATGACA ACCCCACATG ACATCAGACT TCATGGGTCA
    TCAGTTACTC CAGCTTTTGT TGAATTTGAC ACATCCCTTG AAGGGTTTGG CTGCCTGTTC
    CTGCCCAGTC TGGCCCCTCA TAATGCCCCT ACAAACAATG CTGTCACAAC AGGTCTTATC
    GATGGGGCCG TGGTCAGCGG TATTGGTTCT GGTGAACGAT TCTTCCCTCC TCCATCTGGC
    TTAAGCTACT CCAGCTGGTT TTGCATTGAA CATTTTAGTT CTCCTCCCAG TAACCACCCA
    GTCAGACTCC TTACTGTTGT TCGCCGAGCA AATTCTTCTG AGCACCATTA TGTATGCCTT
    GCCATAGTTC TCTCTGCAAA AGATCGGTCT CTGATTGTCT CCACCAAAGA GGAACTACTC
    CAAAATTATG TTGATGATTT TAGTGAAGAA TCCTCTTTCT ATGAGATTCT GCCATGCTGT
    GCTCGCTTCC GTTGTGGAGA ACTGATCGTT GAGGGACAGT GGCATCACCT GGTTCTGGTG
    ATGAGCAAAG GGATGCTGAA GAACAGCACT GCAACCCTCT ACATCGACGG CCAGCTTGTC
    AGCACTGTGA AGCTTCATTA TGTTCACAGT ACTCCCGGGG GCTCAGGCTC TGCAAACCCA
    CCAGTGGTTA GCACAGTCTA TGCCTACATT GGTACTCCGC CTGCCCAGCG CCAAATTGCT
    TCATTGGTTT GGCGCCTGGG ACCTACACAC TTTCTAGAAG AAGTATTACC TCCTTCAAAT
    GTTACTACCA TTTATGAACT AGGACCAAAT TACGTGGGAA GCTTTCAGGC CGTATGTATG
    CCATGTAAAG ATGCAAAATC TGAAGGTGTG GTCCCATCTC CTGTGTCATT AGTACCAGAG
    GAAAAGGTGT CATTCGGCCT CTATGCACTG TCCGTGTCCG CCTTGACAGT GGCGAGAATC
    CGGAAGGTGT ACAACAAGCT GGACAGCAAA GCCATCGCTA AGCAGTTAGG CATTTCCTCC
    CATGAGAACG CCACTCCTGT GAAGCTGATC CACAACTCCG CAGGACACCT CAATGGGCCC
    GCACGGACAA TCGGGGCCAG TCTGATTGGA TACTTGGGAG TAAGAACATT TGTTCCTAAG
    CCTGTTGCCA CTACTTTGCA GTACGTTGGT GGGGCCGCAG CCATCCTGGG CCTGGTAGCC
    ATGGCTTCTG ATGTGGAAGG GCTGTATGCA GCGGTCAAGG CCCTAGTTTG TGTGGTCAAG
    AGTAACCCAC TAGCCAGCAA AGAAATGGAA AGAATCCGGG GCTACCAGTT GCTGGCAATG
    CTGTTTAAGA AGAAACGCTC CCTTCTTAAC AGCCACATTC TCCATCTCAC TTTTTCCTTG
    GTGGGAACTG TTGACAGTGG ACATGAGACT TCCATTATTC CAAATTCAAC AGCTTTCCAG
    GATCTACTTT GTGATTTTGA AGTCTGGCTC CATGCTCCAT ATGAACTTCA TCTCTCCCTA
    TTTGAACACT TTATTGAATT GCTCACAGAG TCCAGTGAAG CTTCAAAGAA TGCCAAGTTG
    ATGAGGGAAT TCCAGCTAAT CCCAAAGCTG CTACTGACTC TTCGAGATAT GTCTCTATCC
    CAGCCTACCA TTGCTGCTAT TAGTAACGTG CTGAGCTTCT TACTGCAGGG TTTTCCCAAC
    AGCAATGATC TACTCAGGTT TGGCCAGTTC ATTTCTTCTA CTTTACCAAC CTTTGCAGTT
    TGTGAGAAGT TTGTAGTTAT GGAAATAAAC CACGAAGAGA AGCCTGACAC TGGAACTGAA
    GAAGAGTTTG GAGGTCTTGT ATCTGCTAAT CTTATACTTT TGAGGAACAG ACTTCTGGAT
    ATCTTACTAA AACTGGTTTA CACATCTAAA GAAAAGACAA GCATTAATTT GCAAGCTTGT
    GAAGAGCTGG TCAGGACGCT GGGCTTTGAC TGGATTATGA TGTTTATGGA AGAACACTTG
    CATTCTACCA CAGTCACAGC AGCCATGAGG ATTCTTGTTG TCCTACTGAG TAATCAGTCT
    ATTCTCATCA AGTTCAAGGA GGGACTCAGT GGTGGAGGCT GGCTGGAGCA GACAGATTCT
    GTCTTAACGA ATAAGATCGG AACTGTATTA GGATTCAATG TGGGCAGGAG TGCTGGCGGA
    CGATCAACTG TCAGGGAGAT TAACCGGGAT GCTTGTCACT TTCCTGGCTT TCCGGTGCTT
    CAGTCATTCC TTCCTAAACA CACTAATGTC CCTGCTCTCT ATTTCCTCCT CATGGCATTG
    TTTCTGCAGC AGCCCGTTAG TGAGCTGCCT GAGAACCTGC AGGTCAGTGT GCCTGTCGTC
    AGCAGCCGAT GTAAGCAGGG TTGCCAGTTT GATTTAGATT CCATCTGGAC CTTTATCTTT
    GGAGTTCCTG CCTCCAGTGG AACCGTGGTC TCATCTATCC ACAATGTGTG CACAGAAGCT
    GCTTTTTTGT TACTGGGCAT GCTCCGAAGC ATGCTGAATT CACCTTGGCA ATCAGAAGAA
    GAGGGGTCTT GGCTCCGGGA GTATCCTGTG ACCCTGATGC AGTTCTTCAG ATACTTGTAT
    CACAATGTCC CGGACCTGGC CTCCATGTGG ATGAACCCTG ATTTCCTGTG TGCACTTGCA
    GCCACGGTCT TCCCCTTCAA CATCCGCCCT TATTCAGAAA TGGTGACTGA TCTCGATGAT
    GAAGTTGGAT CTCCAGCTGA AGAATTTAAA GCCTTTGCAG CAGACACAGG GATGAACAGA
    AGCCAATCAG AGTACTGCAA CGTGGGCGTC AAGACATACC TGACTAATCA CCCAGCTAAG
    AAATTTGTTT TTGACTTCAT GCGGGTCTTA ATAATAGACA ACCTCTGTCT TACACCTGCC
    AGCAAGCAGA CTCCACTGAT TGATCTTTTG TTGGAGGCTT CTCCTGAGAG ATCGACAAGA
    ACGCAGCAAA AAGAATTTCA GACTTACATT TTGGATAGTG TGATGGATCA TTTGCTTGCA
    GCTGATGTTT TACTGGGAGA AGATGCATCT CTTCCTATCA CCAGTGGAGG AAGCTACCAG
    GTATTGGTGA ACAATGTGTT TTATTTCACA CAACGTGTGG TGGACAAGCT TTGGCAAGGC
    ATGTTCAACA AAGAATCTAA ACTTCTTATA GATTTTATAA TTCAACTAAT TGCACAGTCA
    AAGAGAAGAT CCCAGGGGTT GTCACTGGAT GCAGTCTATC ACTGCCTCAA CAGGACTATC
    CTGTACCAGT TCTCCCGGGC GCACAAAACC GTCCCGCAGC AAGTAGCCCT TCTTGACTCT
    CTCAGGGTCC TGACTGTCAA CAGGAACTTG ATCCTGGGCC CCGGAAACCA CGACCAAGAA
    TTCATCAGCT GTCTGGCTCA CTGCCTCATT AACCTGCATG TTGGGAGCAA TGTGGATGGA
    TTTGGACTGG AAGCAGAAGC CCGCATGACT ACATGGCATA TTATGATCCC CTCGGACATT
    GAACCAGATG GTGGTTACAG CCAAGATATT AGTGAAGGGC GTCAGCTTCT CATAAAAGCT
    GTCAACAGAG TTTGGACGGA ATTAATTCAT AGTAAGAAAC AAGTCTTGGA GGAGCTTTTC
    AAAGTCACTC TACCTGTGAA TGAAAGAGGC CACGTGGACA TAGCTATAGC AAGGCCGCTC
    ATCGAAGAAG CTAGTTTGAA GTGCTGGCAG AATCATCTAG CCCATGAAAA GAAGTGCATA
    AGTCGAGGAG AAGCTTTAAT GCCCACCACA CAATCCAAAT TGTCCCGTGT CAGCAGTGGC
    TTTGGTCTTT CCAAGTTAAC GGGATCAAGA AGGAATCGAA AGGAAAGCGG GCTTCATAAA
    CACAGCCTTT CCACCCAGGA GATTTCTCAG TGGATGTTTA CACACATCGC TGTTGTTCGT
    GACTTGGTAG ACACACAATA TAAGGAATAC CAGGAGCGTC AGCAAAATGC CCTGAAGTAC
    GTGACGGAAG AGTGGTGCCA GATCGAGTAT GAGCTGCTGC GGGAGCGGGG ACTCTGGGGC
    CCCCCCATTG GTTCCCATCT CGACAAGTGG ATGTTGGAGA TGACAGAGGG TCCCTGCAGA
    ATGAGGAAAA AAATGGTGCG AAATGATATG TTTTATAACC ATTACCCTTA CGTGCCAGAA
    ACGGAGCAGG AAACCAACGT GGCGTCTGAG ATCCCAAGTA AACAGCCTGA GACACCTGAT
    GATATCATTC CTCAAAAGAA GCCTGCTCGA TACAGAAGAG CCGTAAGTTA TGACAGTAAA
    GAATACTACA TGCGACTAGC CTCCGGCAAC CCAGCCATTG TGCAGGATGC CATCGTAGAG
    AGCTCTGAAG GAGAAGCTGC TCAGCAAGAA CCAGAACATG GTGAAGACAC GATTGCTAAA
    GTCAAAGGAC TGGTCAAGCC ACCTCTCAAA CGGTCTCGGT CAGCACCGGA CGGAGGCGAT
    GAGGAGACAC AGGAGCAGTT ACAGGACCAG ACAGCTGAGG GCAGCTCGAT AGAAGAGGAG
    GAGAAAACAG ACAACGCCAC CCTGCTGCGC CTGTTGGAGG AGGGCGAAAA GATCCAGCAC
    ATGTACCGCT GTGCTCGAGT CCAGGGCCTT GATACCAGTG AGGGGCTACT CCTCTTTGGT
    AAAGAGCATT TCTATGTGAT TGATGGATTT ACAATGACAG CAACCAGAGA AATAAGAGAT
    ATTGAAACCT TACCTCCAAA TATGCATGAG CCGATTATCC CTAGAGGAGC CAGGCAAGGC
    CCCAGTCAGC TCAAGAGAAC GTGCAGTATC TTTGCATATG AAGATATCAA GGAAGTGCAT
    AAACGGAGAT ATCTCCTGCA GCCTATCGCT GTGGAAGTTT TCTCTGGAGA TGGACGAAAC
    TACCTCCTTG CTTTTCAGAA AGGAATCAGA AACAAAGTTT ATCAAAGGTT TTTGGCTGTA
    GTGCCATCTC TCACTGACAG TTCAGAGTCT GTGTCTGGGC AGCGGCCGAA CACCAGCGTG
    GAGCAGGGAT CTGGGTTGCT TAGCACTCTG GTTGGAGAGA AGTCTGTGAC TCAGAGATGG
    GAGAGAGGTG AAATCAGCAA CTTTCAGTAT TTGATGCACT TGAACACTTT GGCTGGAAGA
    TCGTATAATG ACCTCATGCA ATATCCTGTC TTCCCCTGGA TCCTTGCAGA TTACGACTCG
    GAGGAGGTCG ATCTTACTAA TCCCAAGACA TTTAGAAACC TGGCCAAGCC AATGGGAGCA
    CAGACAGATG AACGATTAGC TCAGTATAAG AAGCGATATA AAGACTGGGA AGATCCTAAT
    GGAGAAACTC CAGCCTACCA CTATGGGACC CACTATTCAT CAGCCATGAT TGTGGCTTCA
    TACCTTGTCA GAATGGAGCC CTTCACACAG ATATTCCTAA GGCTACAGGG AGGCCACTTT
    GACCTGGCTG ACCGGATGTT TCACAGCGTG CGTGAGGCCT GGTACTCAGC GTCAAAGCAC
    AACATGGCAG ATGTAAAGGA ACTCATCCCA GAATTTTTTT ACTTACCAGA GTTCCTGTTC
    AATTCCAACA ACTTTGACCT TGGCTGTAAA CAAAACGGCA CCAAACTTGG AGATGTTATC
    CTTCCACCCT GGGCAAAAGG GGACCCACGA GAATTCATCC GGGTGCACCG TGAGGCTCTG
    GAATGTGACT ACGTGAGTGC CCATCTGCAT GAGTGGATCG ACTTAATCTT CGGCTATAAG
    CAGCAAGGTC CTGCCGCAGT AGAAGCTGTA AACGTCTTCC ATCACCTTTT CTATGAGGGT
    CAAGTGGATA TCTACAACAT AAATGACCCC CTCAAGGAAA CAGCCACCAT AGGGTTCATT
    AATAACTTTG GGCAGATCCC TAAACAGTTA TTTAAAAAAC CTCATCCACC CAAGCGAGTG
    AGGAGTCGAC TCAACGGAGA CAATGCGGGG GCCTCCGTCC CGCCAGGGTC TGCCAGTGAC
    AAGATCTTTT TTCATCATCT GGACAACCTG AGGCCTTCAC TAACACCTGT AAAAGAACTC
    AAAGAGCCTG TGGGACAAAT CGTGTGTACA GACAAAGGCA TTCTTGCGGT AGAACAGAAT
    AAGGTTCTTA TCCCACCAAC CTGGAACAAG ACTTTCGCCT GGGGCTACGC AGACCTCAGC
    TGCAGGCTGG GGACCTATGA GTCAGACAAG GCAGTGACTG TTTATGAATG TTTGTCGGAG
    TGGGGCCAGA TTCTCTGTGC GATCTGCCCC AACCCCAAGC TGGTCATCAC GGGTGGAACA
    AGTACGGTGG TGTGTGTTTG GGAGATGAGT ACCTCGAAAG AAAAGGCTAA GACTCTCACT
    CTCAAACAGG CCTTACTTGG CCACACGGAT ACGGTTACCT GTGCCACCGC GTCGTTAGCC
    TATCACATCA TCGTCAGCGG GTCCCGAGAC CGAACCTGCA TCATCTGGGA CCTGAACAAG
    CTGTCATTCC TAACCCAGCT CCGAGGCCAC CGGGCTCCCG TGTCTGCTCT GTGTATCAAT
    GAATTAACCG GGGACATCGT GTCCTGCGCC GGCACGTACA TCCATGTGTG GAGCATAAAC
    GGGAACCCCG TCGTGAGTGT GAACACGTTC ACAGGCAGGA GCCAGCAGAT CGTCTGTTGC
    TGTGTGTCGG AGATGAACGA GTGGGACACG CAGAACGTCA TCGTCACGGG ACACTCGGAT
    GGCGTGGTCC GGTTTTGGAG AATGGAATTT TTGCAAGTTC CTGAAACACC AGCCCCTGAG
    CCTGCTGAAG TCCTCGAAAT GCAGGAAGAC TGTCCAGAAG CACAAATCGG GCAAGAAGCC
    CAGGACGAGG ACAGTAGCGA TTCCGAGGCC GACGAGCAGA GCGTCAGCCA GGACCCCAAG
    GACACGCCCA GCCAGCCCAG CAGCACCAGC CACCGGCCCC GGGCAGCCTC CTGCAGAGCC
    ACAGCCACCT GGTATAACGA CAGTGGCTCC GACGACTCCA GGCGCTGGTC CGATCAGCTC
    AGCCTCGATG AGAAAGACGG CTTCATATTT GTGAACTATT CAGAGGGCCA GAGCAGACCT
    CATCTTCAGA CTCCCCTTAA CCACCCCCAC CCCAATCCCA TCGAGGCGCG GAATTACAGC
    CGATTGAAGC CTGGGTACCG CTGGGAACGG CAGCTCGTGT TCAGGAGTAA GCTGACCATG
    CACACGGCCT TTGACCGGAA GGACAATGCT CACCCTGCCG AGATCACTGC ATTGGGCGTC
    TCCAAGGACC ACAGCAGGAT CCTCGTGGGC GACAGTCGGG GCCGGGTGTT CAGCTGGTCT
    GTGAGCGACC AGCCCGGCCG CTCTGCCGCT GACCACTGGG TGAAGGACGA AGGAGGGGAC
    AGCTGTTCAG GCTGCTCCGT GCGGTTCTCT CTCACGGAAA GACGGCACCA TTGCAGGAAC
    TGTGGTCAGC TCTTCTGCCA GAAGTGCAGC CGATTTCAGT CAGAAATCAA GCGCTTGAAA
    ATCTCCTCCC CAGTACGTGT CTGTCAGAAC TGTTACTACA ACTTACAGCA TGAGCGGAGT
    TCAGAAGACG GGCCTCGGAA TTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90115
    Clone ID Related Accession (Same CDS sequence) XM_010797980.2 , XM_015471704.1 , XM_015463824.1 , XM_015471703.1
    Accession Version XM_015471704.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WD repeat and FYVE domain-containing protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103983.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGTC GTGCAGAACA GATCCCTCCT TGCCTGACAA GTGAGTCTTC TCTTCCCTCT
    CCTTGGGGGA CTCCAGCTTT GTCCAGGAAA AGGCACGCGT ATCATTCTGT TTCTACTCCC
    CCCGTCTACC CTCCTAAAAA CGTTGCTGAC CTGAAACCAC ACGTGGCAAC TTCATCTCTC
    TCAAGTTCTG ATGCCGTCAT CATCCATCCT GGAGCCATGC TTGCTATGCT GGATCTCCTG
    GCCTCTGTTG GGTCAGTGAC ACAGCCAGAA CATGCCTTGG ACCTTCAACT TGCCGTGGCC
    AATATTTTAC AATCCCTGGT GCACACGGAA AGAAACCAGC AGGTCATGTG TGAAGCTGGT
    CTCCATGCAC GACTGCTGCA GCGGTGCAGT GCCGCTCTGG CTGACGAGGA CCACTCGCTG
    CACCCACCCC TGCAGCGCAT GTTCGAGCGA TTAGCCTCTC AAGCGCTGGA GCCCATGGTG
    CTCAGGGAGT TTTTGCGTTT GGCAAGTCCA TTAAATTGTG GTGCCTGGGA CAAAAAACTG
    CTAAAACAAT ATAGGGTCCA CAAACCAAGT TCACTGAGTT TTGAGCCAGA GATGAGAAGC
    AGTATGATCA CATCCATGGA AGGTCTGGGT TCTGATAATG TTTTCAGCTT ACATGAAGAT
    AACCATTACC GGATAAGCAA GAGCCTAGTG AAATCTGCAG AAGGAAGTAC TGTACCCCTA
    ACCAGAGTGA AATGTCTGGT CTCCATGACA ACCCCACATG ACATCAGACT TCATGGGTCA
    TCAGTTACTC CAGCTTTTGT TGAATTTGAC ACATCCCTTG AAGGGTTTGG CTGCCTGTTC
    CTGCCCAGTC TGGCCCCTCA TAATGCCCCT ACAAACAATG CTGTCACAAC AGGTCTTATC
    GATGGGGCCG TGGTCAGCGG TATTGGTTCT GGTGAACGAT TCTTCCCTCC TCCATCTGGC
    TTAAGCTACT CCAGCTGGTT TTGCATTGAA CATTTTAGTT CTCCTCCCAG TAACCACCCA
    GTCAGACTCC TTACTGTTGT TCGCCGAGCA AATTCTTCTG AGCACCATTA TGTATGCCTT
    GCCATAGTTC TCTCTGCAAA AGATCGGTCT CTGATTGTCT CCACCAAAGA GGAACTACTC
    CAAAATTATG TTGATGATTT TAGTGAAGAA TCCTCTTTCT ATGAGATTCT GCCATGCTGT
    GCTCGCTTCC GTTGTGGAGA ACTGATCGTT GAGGGACAGT GGCATCACCT GGTTCTGGTG
    ATGAGCAAAG GGATGCTGAA GAACAGCACT GCAACCCTCT ACATCGACGG CCAGCTTGTC
    AGCACTGTGA AGCTTCATTA TGTTCACAGT ACTCCCGGGG GCTCAGGCTC TGCAAACCCA
    CCAGTGGTTA GCACAGTCTA TGCCTACATT GGTACTCCGC CTGCCCAGCG CCAAATTGCT
    TCATTGGTTT GGCGCCTGGG ACCTACACAC TTTCTAGAAG AAGTATTACC TCCTTCAAAT
    GTTACTACCA TTTATGAACT AGGACCAAAT TACGTGGGAA GCTTTCAGGC CGTATGTATG
    CCATGTAAAG ATGCAAAATC TGAAGGTGTG GTCCCATCTC CTGTGTCATT AGTACCAGAG
    GAAAAGGTGT CATTCGGCCT CTATGCACTG TCCGTGTCCG CCTTGACAGT GGCGAGAATC
    CGGAAGGTGT ACAACAAGCT GGACAGCAAA GCCATCGCTA AGCAGTTAGG CATTTCCTCC
    CATGAGAACG CCACTCCTGT GAAGCTGATC CACAACTCCG CAGGACACCT CAATGGGCCC
    GCACGGACAA TCGGGGCCAG TCTGATTGGA TACTTGGGAG TAAGAACATT TGTTCCTAAG
    CCTGTTGCCA CTACTTTGCA GTACGTTGGT GGGGCCGCAG CCATCCTGGG CCTGGTAGCC
    ATGGCTTCTG ATGTGGAAGG GCTGTATGCA GCGGTCAAGG CCCTAGTTTG TGTGGTCAAG
    AGTAACCCAC TAGCCAGCAA AGAAATGGAA AGAATCCGGG GCTACCAGTT GCTGGCAATG
    CTGTTTAAGA AGAAACGCTC CCTTCTTAAC AGCCACATTC TCCATCTCAC TTTTTCCTTG
    GTGGGAACTG TTGACAGTGG ACATGAGACT TCCATTATTC CAAATTCAAC AGCTTTCCAG
    GATCTACTTT GTGATTTTGA AGTCTGGCTC CATGCTCCAT ATGAACTTCA TCTCTCCCTA
    TTTGAACACT TTATTGAATT GCTCACAGAG TCCAGTGAAG CTTCAAAGAA TGCCAAGTTG
    ATGAGGGAAT TCCAGCTAAT CCCAAAGCTG CTACTGACTC TTCGAGATAT GTCTCTATCC
    CAGCCTACCA TTGCTGCTAT TAGTAACGTG CTGAGCTTCT TACTGCAGGG TTTTCCCAAC
    AGCAATGATC TACTCAGGTT TGGCCAGTTC ATTTCTTCTA CTTTACCAAC CTTTGCAGTT
    TGTGAGAAGT TTGTAGTTAT GGAAATAAAC CACGAAGAGA AGCCTGACAC TGGAACTGAA
    GAAGAGTTTG GAGGTCTTGT ATCTGCTAAT CTTATACTTT TGAGGAACAG ACTTCTGGAT
    ATCTTACTAA AACTGGTTTA CACATCTAAA GAAAAGACAA GCATTAATTT GCAAGCTTGT
    GAAGAGCTGG TCAGGACGCT GGGCTTTGAC TGGATTATGA TGTTTATGGA AGAACACTTG
    CATTCTACCA CAGTCACAGC AGCCATGAGG ATTCTTGTTG TCCTACTGAG TAATCAGTCT
    ATTCTCATCA AGTTCAAGGA GGGACTCAGT GGTGGAGGCT GGCTGGAGCA GACAGATTCT
    GTCTTAACGA ATAAGATCGG AACTGTATTA GGATTCAATG TGGGCAGGAG TGCTGGCGGA
    CGATCAACTG TCAGGGAGAT TAACCGGGAT GCTTGTCACT TTCCTGGCTT TCCGGTGCTT
    CAGTCATTCC TTCCTAAACA CACTAATGTC CCTGCTCTCT ATTTCCTCCT CATGGCATTG
    TTTCTGCAGC AGCCCGTTAG TGAGCTGCCT GAGAACCTGC AGGTCAGTGT GCCTGTCGTC
    AGCAGCCGAT GTAAGCAGGG TTGCCAGTTT GATTTAGATT CCATCTGGAC CTTTATCTTT
    GGAGTTCCTG CCTCCAGTGG AACCGTGGTC TCATCTATCC ACAATGTGTG CACAGAAGCT
    GCTTTTTTGT TACTGGGCAT GCTCCGAAGC ATGCTGAATT CACCTTGGCA ATCAGAAGAA
    GAGGGGTCTT GGCTCCGGGA GTATCCTGTG ACCCTGATGC AGTTCTTCAG ATACTTGTAT
    CACAATGTCC CGGACCTGGC CTCCATGTGG ATGAACCCTG ATTTCCTGTG TGCACTTGCA
    GCCACGGTCT TCCCCTTCAA CATCCGCCCT TATTCAGAAA TGGTGACTGA TCTCGATGAT
    GAAGTTGGAT CTCCAGCTGA AGAATTTAAA GCCTTTGCAG CAGACACAGG GATGAACAGA
    AGCCAATCAG AGTACTGCAA CGTGGGCGTC AAGACATACC TGACTAATCA CCCAGCTAAG
    AAATTTGTTT TTGACTTCAT GCGGGTCTTA ATAATAGACA ACCTCTGTCT TACACCTGCC
    AGCAAGCAGA CTCCACTGAT TGATCTTTTG TTGGAGGCTT CTCCTGAGAG ATCGACAAGA
    ACGCAGCAAA AAGAATTTCA GACTTACATT TTGGATAGTG TGATGGATCA TTTGCTTGCA
    GCTGATGTTT TACTGGGAGA AGATGCATCT CTTCCTATCA CCAGTGGAGG AAGCTACCAG
    GTATTGGTGA ACAATGTGTT TTATTTCACA CAACGTGTGG TGGACAAGCT TTGGCAAGGC
    ATGTTCAACA AAGAATCTAA ACTTCTTATA GATTTTATAA TTCAACTAAT TGCACAGTCA
    AAGAGAAGAT CCCAGGGGTT GTCACTGGAT GCAGTCTATC ACTGCCTCAA CAGGACTATC
    CTGTACCAGT TCTCCCGGGC GCACAAAACC GTCCCGCAGC AAGTAGCCCT TCTTGACTCT
    CTCAGGGTCC TGACTGTCAA CAGGAACTTG ATCCTGGGCC CCGGAAACCA CGACCAAGAA
    TTCATCAGCT GTCTGGCTCA CTGCCTCATT AACCTGCATG TTGGGAGCAA TGTGGATGGA
    TTTGGACTGG AAGCAGAAGC CCGCATGACT ACATGGCATA TTATGATCCC CTCGGACATT
    GAACCAGATG GTGGTTACAG CCAAGATATT AGTGAAGGGC GTCAGCTTCT CATAAAAGCT
    GTCAACAGAG TTTGGACGGA ATTAATTCAT AGTAAGAAAC AAGTCTTGGA GGAGCTTTTC
    AAAGTCACTC TACCTGTGAA TGAAAGAGGC CACGTGGACA TAGCTATAGC AAGGCCGCTC
    ATCGAAGAAG CTAGTTTGAA GTGCTGGCAG AATCATCTAG CCCATGAAAA GAAGTGCATA
    AGTCGAGGAG AAGCTTTAAT GCCCACCACA CAATCCAAAT TGTCCCGTGT CAGCAGTGGC
    TTTGGTCTTT CCAAGTTAAC GGGATCAAGA AGGAATCGAA AGGAAAGCGG GCTTCATAAA
    CACAGCCTTT CCACCCAGGA GATTTCTCAG TGGATGTTTA CACACATCGC TGTTGTTCGT
    GACTTGGTAG ACACACAATA TAAGGAATAC CAGGAGCGTC AGCAAAATGC CCTGAAGTAC
    GTGACGGAAG AGTGGTGCCA GATCGAGTAT GAGCTGCTGC GGGAGCGGGG ACTCTGGGGC
    CCCCCCATTG GTTCCCATCT CGACAAGTGG ATGTTGGAGA TGACAGAGGG TCCCTGCAGA
    ATGAGGAAAA AAATGGTGCG AAATGATATG TTTTATAACC ATTACCCTTA CGTGCCAGAA
    ACGGAGCAGG AAACCAACGT GGCGTCTGAG ATCCCAAGTA AACAGCCTGA GACACCTGAT
    GATATCATTC CTCAAAAGAA GCCTGCTCGA TACAGAAGAG CCGTAAGTTA TGACAGTAAA
    GAATACTACA TGCGACTAGC CTCCGGCAAC CCAGCCATTG TGCAGGATGC CATCGTAGAG
    AGCTCTGAAG GAGAAGCTGC TCAGCAAGAA CCAGAACATG GTGAAGACAC GATTGCTAAA
    GTCAAAGGAC TGGTCAAGCC ACCTCTCAAA CGGTCTCGGT CAGCACCGGA CGGAGGCGAT
    GAGGAGACAC AGGAGCAGTT ACAGGACCAG ACAGCTGAGG GCAGCTCGAT AGAAGAGGAG
    GAGAAAACAG ACAACGCCAC CCTGCTGCGC CTGTTGGAGG AGGGCGAAAA GATCCAGCAC
    ATGTACCGCT GTGCTCGAGT CCAGGGCCTT GATACCAGTG AGGGGCTACT CCTCTTTGGT
    AAAGAGCATT TCTATGTGAT TGATGGATTT ACAATGACAG CAACCAGAGA AATAAGAGAT
    ATTGAAACCT TACCTCCAAA TATGCATGAG CCGATTATCC CTAGAGGAGC CAGGCAAGGC
    CCCAGTCAGC TCAAGAGAAC GTGCAGTATC TTTGCATATG AAGATATCAA GGAAGTGCAT
    AAACGGAGAT ATCTCCTGCA GCCTATCGCT GTGGAAGTTT TCTCTGGAGA TGGACGAAAC
    TACCTCCTTG CTTTTCAGAA AGGAATCAGA AACAAAGTTT ATCAAAGGTT TTTGGCTGTA
    GTGCCATCTC TCACTGACAG TTCAGAGTCT GTGTCTGGGC AGCGGCCGAA CACCAGCGTG
    GAGCAGGGAT CTGGGTTGCT TAGCACTCTG GTTGGAGAGA AGTCTGTGAC TCAGAGATGG
    GAGAGAGGTG AAATCAGCAA CTTTCAGTAT TTGATGCACT TGAACACTTT GGCTGGAAGA
    TCGTATAATG ACCTCATGCA ATATCCTGTC TTCCCCTGGA TCCTTGCAGA TTACGACTCG
    GAGGAGGTCG ATCTTACTAA TCCCAAGACA TTTAGAAACC TGGCCAAGCC AATGGGAGCA
    CAGACAGATG AACGATTAGC TCAGTATAAG AAGCGATATA AAGACTGGGA AGATCCTAAT
    GGAGAAACTC CAGCCTACCA CTATGGGACC CACTATTCAT CAGCCATGAT TGTGGCTTCA
    TACCTTGTCA GAATGGAGCC CTTCACACAG ATATTCCTAA GGCTACAGGG AGGCCACTTT
    GACCTGGCTG ACCGGATGTT TCACAGCGTG CGTGAGGCCT GGTACTCAGC GTCAAAGCAC
    AACATGGCAG ATGTAAAGGA ACTCATCCCA GAATTTTTTT ACTTACCAGA GTTCCTGTTC
    AATTCCAACA ACTTTGACCT TGGCTGTAAA CAAAACGGCA CCAAACTTGG AGATGTTATC
    CTTCCACCCT GGGCAAAAGG GGACCCACGA GAATTCATCC GGGTGCACCG TGAGGCTCTG
    GAATGTGACT ACGTGAGTGC CCATCTGCAT GAGTGGATCG ACTTAATCTT CGGCTATAAG
    CAGCAAGGTC CTGCCGCAGT AGAAGCTGTA AACGTCTTCC ATCACCTTTT CTATGAGGGT
    CAAGTGGATA TCTACAACAT AAATGACCCC CTCAAGGAAA CAGCCACCAT AGGGTTCATT
    AATAACTTTG GGCAGATCCC TAAACAGTTA TTTAAAAAAC CTCATCCACC CAAGCGAGTG
    AGGAGTCGAC TCAACGGAGA CAATGCGGGG GCCTCCGTCC CGCCAGGGTC TGCCAGTGAC
    AAGATCTTTT TTCATCATCT GGACAACCTG AGGCCTTCAC TAACACCTGT AAAAGAACTC
    AAAGAGCCTG TGGGACAAAT CGTGTGTACA GACAAAGGCA TTCTTGCGGT AGAACAGAAT
    AAGGTTCTTA TCCCACCAAC CTGGAACAAG ACTTTCGCCT GGGGCTACGC AGACCTCAGC
    TGCAGGCTGG GGACCTATGA GTCAGACAAG GCAGTGACTG TTTATGAATG TTTGTCGGAG
    TGGGGCCAGA TTCTCTGTGC GATCTGCCCC AACCCCAAGC TGGTCATCAC GGGTGGAACA
    AGTACGGTGG TGTGTGTTTG GGAGATGAGT ACCTCGAAAG AAAAGGCTAA GACTCTCACT
    CTCAAACAGG CCTTACTTGG CCACACGGAT ACGGTTACCT GTGCCACCGC GTCGTTAGCC
    TATCACATCA TCGTCAGCGG GTCCCGAGAC CGAACCTGCA TCATCTGGGA CCTGAACAAG
    CTGTCATTCC TAACCCAGCT CCGAGGCCAC CGGGCTCCCG TGTCTGCTCT GTGTATCAAT
    GAATTAACCG GGGACATCGT GTCCTGCGCC GGCACGTACA TCCATGTGTG GAGCATAAAC
    GGGAACCCCG TCGTGAGTGT GAACACGTTC ACAGGCAGGA GCCAGCAGAT CGTCTGTTGC
    TGTGTGTCGG AGATGAACGA GTGGGACACG CAGAACGTCA TCGTCACGGG ACACTCGGAT
    GGCGTGGTCC GGTTTTGGAG AATGGAATTT TTGCAAGTTC CTGAAACACC AGCCCCTGAG
    CCTGCTGAAG TCCTCGAAAT GCAGGAAGAC TGTCCAGAAG CACAAATCGG GCAAGAAGCC
    CAGGACGAGG ACAGTAGCGA TTCCGAGGCC GACGAGCAGA GCGTCAGCCA GGACCCCAAG
    GACACGCCCA GCCAGCCCAG CAGCACCAGC CACCGGCCCC GGGCAGCCTC CTGCAGAGCC
    ACAGCCACCT GGTATAACGA CAGTGGCTCC GACGACTCCA GGCGCTGGTC CGATCAGCTC
    AGCCTCGATG AGAAAGACGG CTTCATATTT GTGAACTATT CAGAGGGCCA GAGCAGACCT
    CATCTTCAGA CTCCCCTTAA CCACCCCCAC CCCAATCCCA TCGAGGCGCG GAATTACAGC
    CGATTGAAGC CTGGGTACCG CTGGGAACGG CAGCTCGTGT TCAGGAGTAA GCTGACCATG
    CACACGGCCT TTGACCGGAA GGACAATGCT CACCCTGCCG AGATCACTGC ATTGGGCGTC
    TCCAAGGACC ACAGCAGGAT CCTCGTGGGC GACAGTCGGG GCCGGGTGTT CAGCTGGTCT
    GTGAGCGACC AGCCCGGCCG CTCTGCCGCT GACCACTGGG TGAAGGACGA AGGAGGGGAC
    AGCTGTTCAG GCTGCTCCGT GCGGTTCTCT CTCACGGAAA GACGGCACCA TTGCAGGAAC
    TGTGGTCAGC TCTTCTGCCA GAAGTGCAGC CGATTTCAGT CAGAAATCAA GCGCTTGAAA
    ATCTCCTCCC CAGTACGTGT CTGTCAGAAC TGTTACTACA ACTTACAGCA TGAGCGGAGT
    TCAGAAGACG GGCCTCGGAA TTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327190.1
    CDS430..10296
    Translation

    Target ORF information:

    RefSeq Version XM_015471704.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471704.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGTC GTGCAGAACA GATCCCTCCT TGCCTGACAA GTGAGTCTTC TCTTCCCTCT
    CCTTGGGGGA CTCCAGCTTT GTCCAGGAAA AGGCACGCGT ATCATTCTGT TTCTACTCCC
    CCCGTCTACC CTCCTAAAAA CGTTGCTGAC CTGAAACCAC ACGTGGCAAC TTCATCTCTC
    TCAAGTTCTG ATGCCGTCAT CATCCATCCT GGAGCCATGC TTGCTATGCT GGATCTCCTG
    GCCTCTGTTG GGTCAGTGAC ACAGCCAGAA CATGCCTTGG ACCTTCAACT TGCCGTGGCC
    AATATTTTAC AATCCCTGGT GCACACGGAA AGAAACCAGC AGGTCATGTG TGAAGCTGGT
    CTCCATGCAC GACTGCTGCA GCGGTGCAGT GCCGCTCTGG CTGACGAGGA CCACTCGCTG
    CACCCACCCC TGCAGCGCAT GTTCGAGCGA TTAGCCTCTC AAGCGCTGGA GCCCATGGTG
    CTCAGGGAGT TTTTGCGTTT GGCAAGTCCA TTAAATTGTG GTGCCTGGGA CAAAAAACTG
    CTAAAACAAT ATAGGGTCCA CAAACCAAGT TCACTGAGTT TTGAGCCAGA GATGAGAAGC
    AGTATGATCA CATCCATGGA AGGTCTGGGT TCTGATAATG TTTTCAGCTT ACATGAAGAT
    AACCATTACC GGATAAGCAA GAGCCTAGTG AAATCTGCAG AAGGAAGTAC TGTACCCCTA
    ACCAGAGTGA AATGTCTGGT CTCCATGACA ACCCCACATG ACATCAGACT TCATGGGTCA
    TCAGTTACTC CAGCTTTTGT TGAATTTGAC ACATCCCTTG AAGGGTTTGG CTGCCTGTTC
    CTGCCCAGTC TGGCCCCTCA TAATGCCCCT ACAAACAATG CTGTCACAAC AGGTCTTATC
    GATGGGGCCG TGGTCAGCGG TATTGGTTCT GGTGAACGAT TCTTCCCTCC TCCATCTGGC
    TTAAGCTACT CCAGCTGGTT TTGCATTGAA CATTTTAGTT CTCCTCCCAG TAACCACCCA
    GTCAGACTCC TTACTGTTGT TCGCCGAGCA AATTCTTCTG AGCACCATTA TGTATGCCTT
    GCCATAGTTC TCTCTGCAAA AGATCGGTCT CTGATTGTCT CCACCAAAGA GGAACTACTC
    CAAAATTATG TTGATGATTT TAGTGAAGAA TCCTCTTTCT ATGAGATTCT GCCATGCTGT
    GCTCGCTTCC GTTGTGGAGA ACTGATCGTT GAGGGACAGT GGCATCACCT GGTTCTGGTG
    ATGAGCAAAG GGATGCTGAA GAACAGCACT GCAACCCTCT ACATCGACGG CCAGCTTGTC
    AGCACTGTGA AGCTTCATTA TGTTCACAGT ACTCCCGGGG GCTCAGGCTC TGCAAACCCA
    CCAGTGGTTA GCACAGTCTA TGCCTACATT GGTACTCCGC CTGCCCAGCG CCAAATTGCT
    TCATTGGTTT GGCGCCTGGG ACCTACACAC TTTCTAGAAG AAGTATTACC TCCTTCAAAT
    GTTACTACCA TTTATGAACT AGGACCAAAT TACGTGGGAA GCTTTCAGGC CGTATGTATG
    CCATGTAAAG ATGCAAAATC TGAAGGTGTG GTCCCATCTC CTGTGTCATT AGTACCAGAG
    GAAAAGGTGT CATTCGGCCT CTATGCACTG TCCGTGTCCG CCTTGACAGT GGCGAGAATC
    CGGAAGGTGT ACAACAAGCT GGACAGCAAA GCCATCGCTA AGCAGTTAGG CATTTCCTCC
    CATGAGAACG CCACTCCTGT GAAGCTGATC CACAACTCCG CAGGACACCT CAATGGGCCC
    GCACGGACAA TCGGGGCCAG TCTGATTGGA TACTTGGGAG TAAGAACATT TGTTCCTAAG
    CCTGTTGCCA CTACTTTGCA GTACGTTGGT GGGGCCGCAG CCATCCTGGG CCTGGTAGCC
    ATGGCTTCTG ATGTGGAAGG GCTGTATGCA GCGGTCAAGG CCCTAGTTTG TGTGGTCAAG
    AGTAACCCAC TAGCCAGCAA AGAAATGGAA AGAATCCGGG GCTACCAGTT GCTGGCAATG
    CTGTTTAAGA AGAAACGCTC CCTTCTTAAC AGCCACATTC TCCATCTCAC TTTTTCCTTG
    GTGGGAACTG TTGACAGTGG ACATGAGACT TCCATTATTC CAAATTCAAC AGCTTTCCAG
    GATCTACTTT GTGATTTTGA AGTCTGGCTC CATGCTCCAT ATGAACTTCA TCTCTCCCTA
    TTTGAACACT TTATTGAATT GCTCACAGAG TCCAGTGAAG CTTCAAAGAA TGCCAAGTTG
    ATGAGGGAAT TCCAGCTAAT CCCAAAGCTG CTACTGACTC TTCGAGATAT GTCTCTATCC
    CAGCCTACCA TTGCTGCTAT TAGTAACGTG CTGAGCTTCT TACTGCAGGG TTTTCCCAAC
    AGCAATGATC TACTCAGGTT TGGCCAGTTC ATTTCTTCTA CTTTACCAAC CTTTGCAGTT
    TGTGAGAAGT TTGTAGTTAT GGAAATAAAC CACGAAGAGA AGCCTGACAC TGGAACTGAA
    GAAGAGTTTG GAGGTCTTGT ATCTGCTAAT CTTATACTTT TGAGGAACAG ACTTCTGGAT
    ATCTTACTAA AACTGGTTTA CACATCTAAA GAAAAGACAA GCATTAATTT GCAAGCTTGT
    GAAGAGCTGG TCAGGACGCT GGGCTTTGAC TGGATTATGA TGTTTATGGA AGAACACTTG
    CATTCTACCA CAGTCACAGC AGCCATGAGG ATTCTTGTTG TCCTACTGAG TAATCAGTCT
    ATTCTCATCA AGTTCAAGGA GGGACTCAGT GGTGGAGGCT GGCTGGAGCA GACAGATTCT
    GTCTTAACGA ATAAGATCGG AACTGTATTA GGATTCAATG TGGGCAGGAG TGCTGGCGGA
    CGATCAACTG TCAGGGAGAT TAACCGGGAT GCTTGTCACT TTCCTGGCTT TCCGGTGCTT
    CAGTCATTCC TTCCTAAACA CACTAATGTC CCTGCTCTCT ATTTCCTCCT CATGGCATTG
    TTTCTGCAGC AGCCCGTTAG TGAGCTGCCT GAGAACCTGC AGGTCAGTGT GCCTGTCGTC
    AGCAGCCGAT GTAAGCAGGG TTGCCAGTTT GATTTAGATT CCATCTGGAC CTTTATCTTT
    GGAGTTCCTG CCTCCAGTGG AACCGTGGTC TCATCTATCC ACAATGTGTG CACAGAAGCT
    GCTTTTTTGT TACTGGGCAT GCTCCGAAGC ATGCTGAATT CACCTTGGCA ATCAGAAGAA
    GAGGGGTCTT GGCTCCGGGA GTATCCTGTG ACCCTGATGC AGTTCTTCAG ATACTTGTAT
    CACAATGTCC CGGACCTGGC CTCCATGTGG ATGAACCCTG ATTTCCTGTG TGCACTTGCA
    GCCACGGTCT TCCCCTTCAA CATCCGCCCT TATTCAGAAA TGGTGACTGA TCTCGATGAT
    GAAGTTGGAT CTCCAGCTGA AGAATTTAAA GCCTTTGCAG CAGACACAGG GATGAACAGA
    AGCCAATCAG AGTACTGCAA CGTGGGCGTC AAGACATACC TGACTAATCA CCCAGCTAAG
    AAATTTGTTT TTGACTTCAT GCGGGTCTTA ATAATAGACA ACCTCTGTCT TACACCTGCC
    AGCAAGCAGA CTCCACTGAT TGATCTTTTG TTGGAGGCTT CTCCTGAGAG ATCGACAAGA
    ACGCAGCAAA AAGAATTTCA GACTTACATT TTGGATAGTG TGATGGATCA TTTGCTTGCA
    GCTGATGTTT TACTGGGAGA AGATGCATCT CTTCCTATCA CCAGTGGAGG AAGCTACCAG
    GTATTGGTGA ACAATGTGTT TTATTTCACA CAACGTGTGG TGGACAAGCT TTGGCAAGGC
    ATGTTCAACA AAGAATCTAA ACTTCTTATA GATTTTATAA TTCAACTAAT TGCACAGTCA
    AAGAGAAGAT CCCAGGGGTT GTCACTGGAT GCAGTCTATC ACTGCCTCAA CAGGACTATC
    CTGTACCAGT TCTCCCGGGC GCACAAAACC GTCCCGCAGC AAGTAGCCCT TCTTGACTCT
    CTCAGGGTCC TGACTGTCAA CAGGAACTTG ATCCTGGGCC CCGGAAACCA CGACCAAGAA
    TTCATCAGCT GTCTGGCTCA CTGCCTCATT AACCTGCATG TTGGGAGCAA TGTGGATGGA
    TTTGGACTGG AAGCAGAAGC CCGCATGACT ACATGGCATA TTATGATCCC CTCGGACATT
    GAACCAGATG GTGGTTACAG CCAAGATATT AGTGAAGGGC GTCAGCTTCT CATAAAAGCT
    GTCAACAGAG TTTGGACGGA ATTAATTCAT AGTAAGAAAC AAGTCTTGGA GGAGCTTTTC
    AAAGTCACTC TACCTGTGAA TGAAAGAGGC CACGTGGACA TAGCTATAGC AAGGCCGCTC
    ATCGAAGAAG CTAGTTTGAA GTGCTGGCAG AATCATCTAG CCCATGAAAA GAAGTGCATA
    AGTCGAGGAG AAGCTTTAAT GCCCACCACA CAATCCAAAT TGTCCCGTGT CAGCAGTGGC
    TTTGGTCTTT CCAAGTTAAC GGGATCAAGA AGGAATCGAA AGGAAAGCGG GCTTCATAAA
    CACAGCCTTT CCACCCAGGA GATTTCTCAG TGGATGTTTA CACACATCGC TGTTGTTCGT
    GACTTGGTAG ACACACAATA TAAGGAATAC CAGGAGCGTC AGCAAAATGC CCTGAAGTAC
    GTGACGGAAG AGTGGTGCCA GATCGAGTAT GAGCTGCTGC GGGAGCGGGG ACTCTGGGGC
    CCCCCCATTG GTTCCCATCT CGACAAGTGG ATGTTGGAGA TGACAGAGGG TCCCTGCAGA
    ATGAGGAAAA AAATGGTGCG AAATGATATG TTTTATAACC ATTACCCTTA CGTGCCAGAA
    ACGGAGCAGG AAACCAACGT GGCGTCTGAG ATCCCAAGTA AACAGCCTGA GACACCTGAT
    GATATCATTC CTCAAAAGAA GCCTGCTCGA TACAGAAGAG CCGTAAGTTA TGACAGTAAA
    GAATACTACA TGCGACTAGC CTCCGGCAAC CCAGCCATTG TGCAGGATGC CATCGTAGAG
    AGCTCTGAAG GAGAAGCTGC TCAGCAAGAA CCAGAACATG GTGAAGACAC GATTGCTAAA
    GTCAAAGGAC TGGTCAAGCC ACCTCTCAAA CGGTCTCGGT CAGCACCGGA CGGAGGCGAT
    GAGGAGACAC AGGAGCAGTT ACAGGACCAG ACAGCTGAGG GCAGCTCGAT AGAAGAGGAG
    GAGAAAACAG ACAACGCCAC CCTGCTGCGC CTGTTGGAGG AGGGCGAAAA GATCCAGCAC
    ATGTACCGCT GTGCTCGAGT CCAGGGCCTT GATACCAGTG AGGGGCTACT CCTCTTTGGT
    AAAGAGCATT TCTATGTGAT TGATGGATTT ACAATGACAG CAACCAGAGA AATAAGAGAT
    ATTGAAACCT TACCTCCAAA TATGCATGAG CCGATTATCC CTAGAGGAGC CAGGCAAGGC
    CCCAGTCAGC TCAAGAGAAC GTGCAGTATC TTTGCATATG AAGATATCAA GGAAGTGCAT
    AAACGGAGAT ATCTCCTGCA GCCTATCGCT GTGGAAGTTT TCTCTGGAGA TGGACGAAAC
    TACCTCCTTG CTTTTCAGAA AGGAATCAGA AACAAAGTTT ATCAAAGGTT TTTGGCTGTA
    GTGCCATCTC TCACTGACAG TTCAGAGTCT GTGTCTGGGC AGCGGCCGAA CACCAGCGTG
    GAGCAGGGAT CTGGGTTGCT TAGCACTCTG GTTGGAGAGA AGTCTGTGAC TCAGAGATGG
    GAGAGAGGTG AAATCAGCAA CTTTCAGTAT TTGATGCACT TGAACACTTT GGCTGGAAGA
    TCGTATAATG ACCTCATGCA ATATCCTGTC TTCCCCTGGA TCCTTGCAGA TTACGACTCG
    GAGGAGGTCG ATCTTACTAA TCCCAAGACA TTTAGAAACC TGGCCAAGCC AATGGGAGCA
    CAGACAGATG AACGATTAGC TCAGTATAAG AAGCGATATA AAGACTGGGA AGATCCTAAT
    GGAGAAACTC CAGCCTACCA CTATGGGACC CACTATTCAT CAGCCATGAT TGTGGCTTCA
    TACCTTGTCA GAATGGAGCC CTTCACACAG ATATTCCTAA GGCTACAGGG AGGCCACTTT
    GACCTGGCTG ACCGGATGTT TCACAGCGTG CGTGAGGCCT GGTACTCAGC GTCAAAGCAC
    AACATGGCAG ATGTAAAGGA ACTCATCCCA GAATTTTTTT ACTTACCAGA GTTCCTGTTC
    AATTCCAACA ACTTTGACCT TGGCTGTAAA CAAAACGGCA CCAAACTTGG AGATGTTATC
    CTTCCACCCT GGGCAAAAGG GGACCCACGA GAATTCATCC GGGTGCACCG TGAGGCTCTG
    GAATGTGACT ACGTGAGTGC CCATCTGCAT GAGTGGATCG ACTTAATCTT CGGCTATAAG
    CAGCAAGGTC CTGCCGCAGT AGAAGCTGTA AACGTCTTCC ATCACCTTTT CTATGAGGGT
    CAAGTGGATA TCTACAACAT AAATGACCCC CTCAAGGAAA CAGCCACCAT AGGGTTCATT
    AATAACTTTG GGCAGATCCC TAAACAGTTA TTTAAAAAAC CTCATCCACC CAAGCGAGTG
    AGGAGTCGAC TCAACGGAGA CAATGCGGGG GCCTCCGTCC CGCCAGGGTC TGCCAGTGAC
    AAGATCTTTT TTCATCATCT GGACAACCTG AGGCCTTCAC TAACACCTGT AAAAGAACTC
    AAAGAGCCTG TGGGACAAAT CGTGTGTACA GACAAAGGCA TTCTTGCGGT AGAACAGAAT
    AAGGTTCTTA TCCCACCAAC CTGGAACAAG ACTTTCGCCT GGGGCTACGC AGACCTCAGC
    TGCAGGCTGG GGACCTATGA GTCAGACAAG GCAGTGACTG TTTATGAATG TTTGTCGGAG
    TGGGGCCAGA TTCTCTGTGC GATCTGCCCC AACCCCAAGC TGGTCATCAC GGGTGGAACA
    AGTACGGTGG TGTGTGTTTG GGAGATGAGT ACCTCGAAAG AAAAGGCTAA GACTCTCACT
    CTCAAACAGG CCTTACTTGG CCACACGGAT ACGGTTACCT GTGCCACCGC GTCGTTAGCC
    TATCACATCA TCGTCAGCGG GTCCCGAGAC CGAACCTGCA TCATCTGGGA CCTGAACAAG
    CTGTCATTCC TAACCCAGCT CCGAGGCCAC CGGGCTCCCG TGTCTGCTCT GTGTATCAAT
    GAATTAACCG GGGACATCGT GTCCTGCGCC GGCACGTACA TCCATGTGTG GAGCATAAAC
    GGGAACCCCG TCGTGAGTGT GAACACGTTC ACAGGCAGGA GCCAGCAGAT CGTCTGTTGC
    TGTGTGTCGG AGATGAACGA GTGGGACACG CAGAACGTCA TCGTCACGGG ACACTCGGAT
    GGCGTGGTCC GGTTTTGGAG AATGGAATTT TTGCAAGTTC CTGAAACACC AGCCCCTGAG
    CCTGCTGAAG TCCTCGAAAT GCAGGAAGAC TGTCCAGAAG CACAAATCGG GCAAGAAGCC
    CAGGACGAGG ACAGTAGCGA TTCCGAGGCC GACGAGCAGA GCGTCAGCCA GGACCCCAAG
    GACACGCCCA GCCAGCCCAG CAGCACCAGC CACCGGCCCC GGGCAGCCTC CTGCAGAGCC
    ACAGCCACCT GGTATAACGA CAGTGGCTCC GACGACTCCA GGCGCTGGTC CGATCAGCTC
    AGCCTCGATG AGAAAGACGG CTTCATATTT GTGAACTATT CAGAGGGCCA GAGCAGACCT
    CATCTTCAGA CTCCCCTTAA CCACCCCCAC CCCAATCCCA TCGAGGCGCG GAATTACAGC
    CGATTGAAGC CTGGGTACCG CTGGGAACGG CAGCTCGTGT TCAGGAGTAA GCTGACCATG
    CACACGGCCT TTGACCGGAA GGACAATGCT CACCCTGCCG AGATCACTGC ATTGGGCGTC
    TCCAAGGACC ACAGCAGGAT CCTCGTGGGC GACAGTCGGG GCCGGGTGTT CAGCTGGTCT
    GTGAGCGACC AGCCCGGCCG CTCTGCCGCT GACCACTGGG TGAAGGACGA AGGAGGGGAC
    AGCTGTTCAG GCTGCTCCGT GCGGTTCTCT CTCACGGAAA GACGGCACCA TTGCAGGAAC
    TGTGGTCAGC TCTTCTGCCA GAAGTGCAGC CGATTTCAGT CAGAAATCAA GCGCTTGAAA
    ATCTCCTCCC CAGTACGTGT CTGTCAGAAC TGTTACTACA ACTTACAGCA TGAGCGGAGT
    TCAGAAGACG GGCCTCGGAA TTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90115
    Clone ID Related Accession (Same CDS sequence) XM_010797980.2 , XM_015471704.1 , XM_015463824.1 , XM_015471703.1
    Accession Version XM_015463824.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WD repeat and FYVE domain-containing protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644958.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGTC GTGCAGAACA GATCCCTCCT TGCCTGACAA GTGAGTCTTC TCTTCCCTCT
    CCTTGGGGGA CTCCAGCTTT GTCCAGGAAA AGGCACGCGT ATCATTCTGT TTCTACTCCC
    CCCGTCTACC CTCCTAAAAA CGTTGCTGAC CTGAAACCAC ACGTGGCAAC TTCATCTCTC
    TCAAGTTCTG ATGCCGTCAT CATCCATCCT GGAGCCATGC TTGCTATGCT GGATCTCCTG
    GCCTCTGTTG GGTCAGTGAC ACAGCCAGAA CATGCCTTGG ACCTTCAACT TGCCGTGGCC
    AATATTTTAC AATCCCTGGT GCACACGGAA AGAAACCAGC AGGTCATGTG TGAAGCTGGT
    CTCCATGCAC GACTGCTGCA GCGGTGCAGT GCCGCTCTGG CTGACGAGGA CCACTCGCTG
    CACCCACCCC TGCAGCGCAT GTTCGAGCGA TTAGCCTCTC AAGCGCTGGA GCCCATGGTG
    CTCAGGGAGT TTTTGCGTTT GGCAAGTCCA TTAAATTGTG GTGCCTGGGA CAAAAAACTG
    CTAAAACAAT ATAGGGTCCA CAAACCAAGT TCACTGAGTT TTGAGCCAGA GATGAGAAGC
    AGTATGATCA CATCCATGGA AGGTCTGGGT TCTGATAATG TTTTCAGCTT ACATGAAGAT
    AACCATTACC GGATAAGCAA GAGCCTAGTG AAATCTGCAG AAGGAAGTAC TGTACCCCTA
    ACCAGAGTGA AATGTCTGGT CTCCATGACA ACCCCACATG ACATCAGACT TCATGGGTCA
    TCAGTTACTC CAGCTTTTGT TGAATTTGAC ACATCCCTTG AAGGGTTTGG CTGCCTGTTC
    CTGCCCAGTC TGGCCCCTCA TAATGCCCCT ACAAACAATG CTGTCACAAC AGGTCTTATC
    GATGGGGCCG TGGTCAGCGG TATTGGTTCT GGTGAACGAT TCTTCCCTCC TCCATCTGGC
    TTAAGCTACT CCAGCTGGTT TTGCATTGAA CATTTTAGTT CTCCTCCCAG TAACCACCCA
    GTCAGACTCC TTACTGTTGT TCGCCGAGCA AATTCTTCTG AGCACCATTA TGTATGCCTT
    GCCATAGTTC TCTCTGCAAA AGATCGGTCT CTGATTGTCT CCACCAAAGA GGAACTACTC
    CAAAATTATG TTGATGATTT TAGTGAAGAA TCCTCTTTCT ATGAGATTCT GCCATGCTGT
    GCTCGCTTCC GTTGTGGAGA ACTGATCGTT GAGGGACAGT GGCATCACCT GGTTCTGGTG
    ATGAGCAAAG GGATGCTGAA GAACAGCACT GCAACCCTCT ACATCGACGG CCAGCTTGTC
    AGCACTGTGA AGCTTCATTA TGTTCACAGT ACTCCCGGGG GCTCAGGCTC TGCAAACCCA
    CCAGTGGTTA GCACAGTCTA TGCCTACATT GGTACTCCGC CTGCCCAGCG CCAAATTGCT
    TCATTGGTTT GGCGCCTGGG ACCTACACAC TTTCTAGAAG AAGTATTACC TCCTTCAAAT
    GTTACTACCA TTTATGAACT AGGACCAAAT TACGTGGGAA GCTTTCAGGC CGTATGTATG
    CCATGTAAAG ATGCAAAATC TGAAGGTGTG GTCCCATCTC CTGTGTCATT AGTACCAGAG
    GAAAAGGTGT CATTCGGCCT CTATGCACTG TCCGTGTCCG CCTTGACAGT GGCGAGAATC
    CGGAAGGTGT ACAACAAGCT GGACAGCAAA GCCATCGCTA AGCAGTTAGG CATTTCCTCC
    CATGAGAACG CCACTCCTGT GAAGCTGATC CACAACTCCG CAGGACACCT CAATGGGCCC
    GCACGGACAA TCGGGGCCAG TCTGATTGGA TACTTGGGAG TAAGAACATT TGTTCCTAAG
    CCTGTTGCCA CTACTTTGCA GTACGTTGGT GGGGCCGCAG CCATCCTGGG CCTGGTAGCC
    ATGGCTTCTG ATGTGGAAGG GCTGTATGCA GCGGTCAAGG CCCTAGTTTG TGTGGTCAAG
    AGTAACCCAC TAGCCAGCAA AGAAATGGAA AGAATCCGGG GCTACCAGTT GCTGGCAATG
    CTGTTTAAGA AGAAACGCTC CCTTCTTAAC AGCCACATTC TCCATCTCAC TTTTTCCTTG
    GTGGGAACTG TTGACAGTGG ACATGAGACT TCCATTATTC CAAATTCAAC AGCTTTCCAG
    GATCTACTTT GTGATTTTGA AGTCTGGCTC CATGCTCCAT ATGAACTTCA TCTCTCCCTA
    TTTGAACACT TTATTGAATT GCTCACAGAG TCCAGTGAAG CTTCAAAGAA TGCCAAGTTG
    ATGAGGGAAT TCCAGCTAAT CCCAAAGCTG CTACTGACTC TTCGAGATAT GTCTCTATCC
    CAGCCTACCA TTGCTGCTAT TAGTAACGTG CTGAGCTTCT TACTGCAGGG TTTTCCCAAC
    AGCAATGATC TACTCAGGTT TGGCCAGTTC ATTTCTTCTA CTTTACCAAC CTTTGCAGTT
    TGTGAGAAGT TTGTAGTTAT GGAAATAAAC CACGAAGAGA AGCCTGACAC TGGAACTGAA
    GAAGAGTTTG GAGGTCTTGT ATCTGCTAAT CTTATACTTT TGAGGAACAG ACTTCTGGAT
    ATCTTACTAA AACTGGTTTA CACATCTAAA GAAAAGACAA GCATTAATTT GCAAGCTTGT
    GAAGAGCTGG TCAGGACGCT GGGCTTTGAC TGGATTATGA TGTTTATGGA AGAACACTTG
    CATTCTACCA CAGTCACAGC AGCCATGAGG ATTCTTGTTG TCCTACTGAG TAATCAGTCT
    ATTCTCATCA AGTTCAAGGA GGGACTCAGT GGTGGAGGCT GGCTGGAGCA GACAGATTCT
    GTCTTAACGA ATAAGATCGG AACTGTATTA GGATTCAATG TGGGCAGGAG TGCTGGCGGA
    CGATCAACTG TCAGGGAGAT TAACCGGGAT GCTTGTCACT TTCCTGGCTT TCCGGTGCTT
    CAGTCATTCC TTCCTAAACA CACTAATGTC CCTGCTCTCT ATTTCCTCCT CATGGCATTG
    TTTCTGCAGC AGCCCGTTAG TGAGCTGCCT GAGAACCTGC AGGTCAGTGT GCCTGTCGTC
    AGCAGCCGAT GTAAGCAGGG TTGCCAGTTT GATTTAGATT CCATCTGGAC CTTTATCTTT
    GGAGTTCCTG CCTCCAGTGG AACCGTGGTC TCATCTATCC ACAATGTGTG CACAGAAGCT
    GCTTTTTTGT TACTGGGCAT GCTCCGAAGC ATGCTGAATT CACCTTGGCA ATCAGAAGAA
    GAGGGGTCTT GGCTCCGGGA GTATCCTGTG ACCCTGATGC AGTTCTTCAG ATACTTGTAT
    CACAATGTCC CGGACCTGGC CTCCATGTGG ATGAACCCTG ATTTCCTGTG TGCACTTGCA
    GCCACGGTCT TCCCCTTCAA CATCCGCCCT TATTCAGAAA TGGTGACTGA TCTCGATGAT
    GAAGTTGGAT CTCCAGCTGA AGAATTTAAA GCCTTTGCAG CAGACACAGG GATGAACAGA
    AGCCAATCAG AGTACTGCAA CGTGGGCGTC AAGACATACC TGACTAATCA CCCAGCTAAG
    AAATTTGTTT TTGACTTCAT GCGGGTCTTA ATAATAGACA ACCTCTGTCT TACACCTGCC
    AGCAAGCAGA CTCCACTGAT TGATCTTTTG TTGGAGGCTT CTCCTGAGAG ATCGACAAGA
    ACGCAGCAAA AAGAATTTCA GACTTACATT TTGGATAGTG TGATGGATCA TTTGCTTGCA
    GCTGATGTTT TACTGGGAGA AGATGCATCT CTTCCTATCA CCAGTGGAGG AAGCTACCAG
    GTATTGGTGA ACAATGTGTT TTATTTCACA CAACGTGTGG TGGACAAGCT TTGGCAAGGC
    ATGTTCAACA AAGAATCTAA ACTTCTTATA GATTTTATAA TTCAACTAAT TGCACAGTCA
    AAGAGAAGAT CCCAGGGGTT GTCACTGGAT GCAGTCTATC ACTGCCTCAA CAGGACTATC
    CTGTACCAGT TCTCCCGGGC GCACAAAACC GTCCCGCAGC AAGTAGCCCT TCTTGACTCT
    CTCAGGGTCC TGACTGTCAA CAGGAACTTG ATCCTGGGCC CCGGAAACCA CGACCAAGAA
    TTCATCAGCT GTCTGGCTCA CTGCCTCATT AACCTGCATG TTGGGAGCAA TGTGGATGGA
    TTTGGACTGG AAGCAGAAGC CCGCATGACT ACATGGCATA TTATGATCCC CTCGGACATT
    GAACCAGATG GTGGTTACAG CCAAGATATT AGTGAAGGGC GTCAGCTTCT CATAAAAGCT
    GTCAACAGAG TTTGGACGGA ATTAATTCAT AGTAAGAAAC AAGTCTTGGA GGAGCTTTTC
    AAAGTCACTC TACCTGTGAA TGAAAGAGGC CACGTGGACA TAGCTATAGC AAGGCCGCTC
    ATCGAAGAAG CTAGTTTGAA GTGCTGGCAG AATCATCTAG CCCATGAAAA GAAGTGCATA
    AGTCGAGGAG AAGCTTTAAT GCCCACCACA CAATCCAAAT TGTCCCGTGT CAGCAGTGGC
    TTTGGTCTTT CCAAGTTAAC GGGATCAAGA AGGAATCGAA AGGAAAGCGG GCTTCATAAA
    CACAGCCTTT CCACCCAGGA GATTTCTCAG TGGATGTTTA CACACATCGC TGTTGTTCGT
    GACTTGGTAG ACACACAATA TAAGGAATAC CAGGAGCGTC AGCAAAATGC CCTGAAGTAC
    GTGACGGAAG AGTGGTGCCA GATCGAGTAT GAGCTGCTGC GGGAGCGGGG ACTCTGGGGC
    CCCCCCATTG GTTCCCATCT CGACAAGTGG ATGTTGGAGA TGACAGAGGG TCCCTGCAGA
    ATGAGGAAAA AAATGGTGCG AAATGATATG TTTTATAACC ATTACCCTTA CGTGCCAGAA
    ACGGAGCAGG AAACCAACGT GGCGTCTGAG ATCCCAAGTA AACAGCCTGA GACACCTGAT
    GATATCATTC CTCAAAAGAA GCCTGCTCGA TACAGAAGAG CCGTAAGTTA TGACAGTAAA
    GAATACTACA TGCGACTAGC CTCCGGCAAC CCAGCCATTG TGCAGGATGC CATCGTAGAG
    AGCTCTGAAG GAGAAGCTGC TCAGCAAGAA CCAGAACATG GTGAAGACAC GATTGCTAAA
    GTCAAAGGAC TGGTCAAGCC ACCTCTCAAA CGGTCTCGGT CAGCACCGGA CGGAGGCGAT
    GAGGAGACAC AGGAGCAGTT ACAGGACCAG ACAGCTGAGG GCAGCTCGAT AGAAGAGGAG
    GAGAAAACAG ACAACGCCAC CCTGCTGCGC CTGTTGGAGG AGGGCGAAAA GATCCAGCAC
    ATGTACCGCT GTGCTCGAGT CCAGGGCCTT GATACCAGTG AGGGGCTACT CCTCTTTGGT
    AAAGAGCATT TCTATGTGAT TGATGGATTT ACAATGACAG CAACCAGAGA AATAAGAGAT
    ATTGAAACCT TACCTCCAAA TATGCATGAG CCGATTATCC CTAGAGGAGC CAGGCAAGGC
    CCCAGTCAGC TCAAGAGAAC GTGCAGTATC TTTGCATATG AAGATATCAA GGAAGTGCAT
    AAACGGAGAT ATCTCCTGCA GCCTATCGCT GTGGAAGTTT TCTCTGGAGA TGGACGAAAC
    TACCTCCTTG CTTTTCAGAA AGGAATCAGA AACAAAGTTT ATCAAAGGTT TTTGGCTGTA
    GTGCCATCTC TCACTGACAG TTCAGAGTCT GTGTCTGGGC AGCGGCCGAA CACCAGCGTG
    GAGCAGGGAT CTGGGTTGCT TAGCACTCTG GTTGGAGAGA AGTCTGTGAC TCAGAGATGG
    GAGAGAGGTG AAATCAGCAA CTTTCAGTAT TTGATGCACT TGAACACTTT GGCTGGAAGA
    TCGTATAATG ACCTCATGCA ATATCCTGTC TTCCCCTGGA TCCTTGCAGA TTACGACTCG
    GAGGAGGTCG ATCTTACTAA TCCCAAGACA TTTAGAAACC TGGCCAAGCC AATGGGAGCA
    CAGACAGATG AACGATTAGC TCAGTATAAG AAGCGATATA AAGACTGGGA AGATCCTAAT
    GGAGAAACTC CAGCCTACCA CTATGGGACC CACTATTCAT CAGCCATGAT TGTGGCTTCA
    TACCTTGTCA GAATGGAGCC CTTCACACAG ATATTCCTAA GGCTACAGGG AGGCCACTTT
    GACCTGGCTG ACCGGATGTT TCACAGCGTG CGTGAGGCCT GGTACTCAGC GTCAAAGCAC
    AACATGGCAG ATGTAAAGGA ACTCATCCCA GAATTTTTTT ACTTACCAGA GTTCCTGTTC
    AATTCCAACA ACTTTGACCT TGGCTGTAAA CAAAACGGCA CCAAACTTGG AGATGTTATC
    CTTCCACCCT GGGCAAAAGG GGACCCACGA GAATTCATCC GGGTGCACCG TGAGGCTCTG
    GAATGTGACT ACGTGAGTGC CCATCTGCAT GAGTGGATCG ACTTAATCTT CGGCTATAAG
    CAGCAAGGTC CTGCCGCAGT AGAAGCTGTA AACGTCTTCC ATCACCTTTT CTATGAGGGT
    CAAGTGGATA TCTACAACAT AAATGACCCC CTCAAGGAAA CAGCCACCAT AGGGTTCATT
    AATAACTTTG GGCAGATCCC TAAACAGTTA TTTAAAAAAC CTCATCCACC CAAGCGAGTG
    AGGAGTCGAC TCAACGGAGA CAATGCGGGG GCCTCCGTCC CGCCAGGGTC TGCCAGTGAC
    AAGATCTTTT TTCATCATCT GGACAACCTG AGGCCTTCAC TAACACCTGT AAAAGAACTC
    AAAGAGCCTG TGGGACAAAT CGTGTGTACA GACAAAGGCA TTCTTGCGGT AGAACAGAAT
    AAGGTTCTTA TCCCACCAAC CTGGAACAAG ACTTTCGCCT GGGGCTACGC AGACCTCAGC
    TGCAGGCTGG GGACCTATGA GTCAGACAAG GCAGTGACTG TTTATGAATG TTTGTCGGAG
    TGGGGCCAGA TTCTCTGTGC GATCTGCCCC AACCCCAAGC TGGTCATCAC GGGTGGAACA
    AGTACGGTGG TGTGTGTTTG GGAGATGAGT ACCTCGAAAG AAAAGGCTAA GACTCTCACT
    CTCAAACAGG CCTTACTTGG CCACACGGAT ACGGTTACCT GTGCCACCGC GTCGTTAGCC
    TATCACATCA TCGTCAGCGG GTCCCGAGAC CGAACCTGCA TCATCTGGGA CCTGAACAAG
    CTGTCATTCC TAACCCAGCT CCGAGGCCAC CGGGCTCCCG TGTCTGCTCT GTGTATCAAT
    GAATTAACCG GGGACATCGT GTCCTGCGCC GGCACGTACA TCCATGTGTG GAGCATAAAC
    GGGAACCCCG TCGTGAGTGT GAACACGTTC ACAGGCAGGA GCCAGCAGAT CGTCTGTTGC
    TGTGTGTCGG AGATGAACGA GTGGGACACG CAGAACGTCA TCGTCACGGG ACACTCGGAT
    GGCGTGGTCC GGTTTTGGAG AATGGAATTT TTGCAAGTTC CTGAAACACC AGCCCCTGAG
    CCTGCTGAAG TCCTCGAAAT GCAGGAAGAC TGTCCAGAAG CACAAATCGG GCAAGAAGCC
    CAGGACGAGG ACAGTAGCGA TTCCGAGGCC GACGAGCAGA GCGTCAGCCA GGACCCCAAG
    GACACGCCCA GCCAGCCCAG CAGCACCAGC CACCGGCCCC GGGCAGCCTC CTGCAGAGCC
    ACAGCCACCT GGTATAACGA CAGTGGCTCC GACGACTCCA GGCGCTGGTC CGATCAGCTC
    AGCCTCGATG AGAAAGACGG CTTCATATTT GTGAACTATT CAGAGGGCCA GAGCAGACCT
    CATCTTCAGA CTCCCCTTAA CCACCCCCAC CCCAATCCCA TCGAGGCGCG GAATTACAGC
    CGATTGAAGC CTGGGTACCG CTGGGAACGG CAGCTCGTGT TCAGGAGTAA GCTGACCATG
    CACACGGCCT TTGACCGGAA GGACAATGCT CACCCTGCCG AGATCACTGC ATTGGGCGTC
    TCCAAGGACC ACAGCAGGAT CCTCGTGGGC GACAGTCGGG GCCGGGTGTT CAGCTGGTCT
    GTGAGCGACC AGCCCGGCCG CTCTGCCGCT GACCACTGGG TGAAGGACGA AGGAGGGGAC
    AGCTGTTCAG GCTGCTCCGT GCGGTTCTCT CTCACGGAAA GACGGCACCA TTGCAGGAAC
    TGTGGTCAGC TCTTCTGCCA GAAGTGCAGC CGATTTCAGT CAGAAATCAA GCGCTTGAAA
    ATCTCCTCCC CAGTACGTGT CTGTCAGAAC TGTTACTACA ACTTACAGCA TGAGCGGAGT
    TCAGAAGACG GGCCTCGGAA TTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319310.1
    CDS430..10296
    Translation

    Target ORF information:

    RefSeq Version XM_015463824.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463824.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGTC GTGCAGAACA GATCCCTCCT TGCCTGACAA GTGAGTCTTC TCTTCCCTCT
    CCTTGGGGGA CTCCAGCTTT GTCCAGGAAA AGGCACGCGT ATCATTCTGT TTCTACTCCC
    CCCGTCTACC CTCCTAAAAA CGTTGCTGAC CTGAAACCAC ACGTGGCAAC TTCATCTCTC
    TCAAGTTCTG ATGCCGTCAT CATCCATCCT GGAGCCATGC TTGCTATGCT GGATCTCCTG
    GCCTCTGTTG GGTCAGTGAC ACAGCCAGAA CATGCCTTGG ACCTTCAACT TGCCGTGGCC
    AATATTTTAC AATCCCTGGT GCACACGGAA AGAAACCAGC AGGTCATGTG TGAAGCTGGT
    CTCCATGCAC GACTGCTGCA GCGGTGCAGT GCCGCTCTGG CTGACGAGGA CCACTCGCTG
    CACCCACCCC TGCAGCGCAT GTTCGAGCGA TTAGCCTCTC AAGCGCTGGA GCCCATGGTG
    CTCAGGGAGT TTTTGCGTTT GGCAAGTCCA TTAAATTGTG GTGCCTGGGA CAAAAAACTG
    CTAAAACAAT ATAGGGTCCA CAAACCAAGT TCACTGAGTT TTGAGCCAGA GATGAGAAGC
    AGTATGATCA CATCCATGGA AGGTCTGGGT TCTGATAATG TTTTCAGCTT ACATGAAGAT
    AACCATTACC GGATAAGCAA GAGCCTAGTG AAATCTGCAG AAGGAAGTAC TGTACCCCTA
    ACCAGAGTGA AATGTCTGGT CTCCATGACA ACCCCACATG ACATCAGACT TCATGGGTCA
    TCAGTTACTC CAGCTTTTGT TGAATTTGAC ACATCCCTTG AAGGGTTTGG CTGCCTGTTC
    CTGCCCAGTC TGGCCCCTCA TAATGCCCCT ACAAACAATG CTGTCACAAC AGGTCTTATC
    GATGGGGCCG TGGTCAGCGG TATTGGTTCT GGTGAACGAT TCTTCCCTCC TCCATCTGGC
    TTAAGCTACT CCAGCTGGTT TTGCATTGAA CATTTTAGTT CTCCTCCCAG TAACCACCCA
    GTCAGACTCC TTACTGTTGT TCGCCGAGCA AATTCTTCTG AGCACCATTA TGTATGCCTT
    GCCATAGTTC TCTCTGCAAA AGATCGGTCT CTGATTGTCT CCACCAAAGA GGAACTACTC
    CAAAATTATG TTGATGATTT TAGTGAAGAA TCCTCTTTCT ATGAGATTCT GCCATGCTGT
    GCTCGCTTCC GTTGTGGAGA ACTGATCGTT GAGGGACAGT GGCATCACCT GGTTCTGGTG
    ATGAGCAAAG GGATGCTGAA GAACAGCACT GCAACCCTCT ACATCGACGG CCAGCTTGTC
    AGCACTGTGA AGCTTCATTA TGTTCACAGT ACTCCCGGGG GCTCAGGCTC TGCAAACCCA
    CCAGTGGTTA GCACAGTCTA TGCCTACATT GGTACTCCGC CTGCCCAGCG CCAAATTGCT
    TCATTGGTTT GGCGCCTGGG ACCTACACAC TTTCTAGAAG AAGTATTACC TCCTTCAAAT
    GTTACTACCA TTTATGAACT AGGACCAAAT TACGTGGGAA GCTTTCAGGC CGTATGTATG
    CCATGTAAAG ATGCAAAATC TGAAGGTGTG GTCCCATCTC CTGTGTCATT AGTACCAGAG
    GAAAAGGTGT CATTCGGCCT CTATGCACTG TCCGTGTCCG CCTTGACAGT GGCGAGAATC
    CGGAAGGTGT ACAACAAGCT GGACAGCAAA GCCATCGCTA AGCAGTTAGG CATTTCCTCC
    CATGAGAACG CCACTCCTGT GAAGCTGATC CACAACTCCG CAGGACACCT CAATGGGCCC
    GCACGGACAA TCGGGGCCAG TCTGATTGGA TACTTGGGAG TAAGAACATT TGTTCCTAAG
    CCTGTTGCCA CTACTTTGCA GTACGTTGGT GGGGCCGCAG CCATCCTGGG CCTGGTAGCC
    ATGGCTTCTG ATGTGGAAGG GCTGTATGCA GCGGTCAAGG CCCTAGTTTG TGTGGTCAAG
    AGTAACCCAC TAGCCAGCAA AGAAATGGAA AGAATCCGGG GCTACCAGTT GCTGGCAATG
    CTGTTTAAGA AGAAACGCTC CCTTCTTAAC AGCCACATTC TCCATCTCAC TTTTTCCTTG
    GTGGGAACTG TTGACAGTGG ACATGAGACT TCCATTATTC CAAATTCAAC AGCTTTCCAG
    GATCTACTTT GTGATTTTGA AGTCTGGCTC CATGCTCCAT ATGAACTTCA TCTCTCCCTA
    TTTGAACACT TTATTGAATT GCTCACAGAG TCCAGTGAAG CTTCAAAGAA TGCCAAGTTG
    ATGAGGGAAT TCCAGCTAAT CCCAAAGCTG CTACTGACTC TTCGAGATAT GTCTCTATCC
    CAGCCTACCA TTGCTGCTAT TAGTAACGTG CTGAGCTTCT TACTGCAGGG TTTTCCCAAC
    AGCAATGATC TACTCAGGTT TGGCCAGTTC ATTTCTTCTA CTTTACCAAC CTTTGCAGTT
    TGTGAGAAGT TTGTAGTTAT GGAAATAAAC CACGAAGAGA AGCCTGACAC TGGAACTGAA
    GAAGAGTTTG GAGGTCTTGT ATCTGCTAAT CTTATACTTT TGAGGAACAG ACTTCTGGAT
    ATCTTACTAA AACTGGTTTA CACATCTAAA GAAAAGACAA GCATTAATTT GCAAGCTTGT
    GAAGAGCTGG TCAGGACGCT GGGCTTTGAC TGGATTATGA TGTTTATGGA AGAACACTTG
    CATTCTACCA CAGTCACAGC AGCCATGAGG ATTCTTGTTG TCCTACTGAG TAATCAGTCT
    ATTCTCATCA AGTTCAAGGA GGGACTCAGT GGTGGAGGCT GGCTGGAGCA GACAGATTCT
    GTCTTAACGA ATAAGATCGG AACTGTATTA GGATTCAATG TGGGCAGGAG TGCTGGCGGA
    CGATCAACTG TCAGGGAGAT TAACCGGGAT GCTTGTCACT TTCCTGGCTT TCCGGTGCTT
    CAGTCATTCC TTCCTAAACA CACTAATGTC CCTGCTCTCT ATTTCCTCCT CATGGCATTG
    TTTCTGCAGC AGCCCGTTAG TGAGCTGCCT GAGAACCTGC AGGTCAGTGT GCCTGTCGTC
    AGCAGCCGAT GTAAGCAGGG TTGCCAGTTT GATTTAGATT CCATCTGGAC CTTTATCTTT
    GGAGTTCCTG CCTCCAGTGG AACCGTGGTC TCATCTATCC ACAATGTGTG CACAGAAGCT
    GCTTTTTTGT TACTGGGCAT GCTCCGAAGC ATGCTGAATT CACCTTGGCA ATCAGAAGAA
    GAGGGGTCTT GGCTCCGGGA GTATCCTGTG ACCCTGATGC AGTTCTTCAG ATACTTGTAT
    CACAATGTCC CGGACCTGGC CTCCATGTGG ATGAACCCTG ATTTCCTGTG TGCACTTGCA
    GCCACGGTCT TCCCCTTCAA CATCCGCCCT TATTCAGAAA TGGTGACTGA TCTCGATGAT
    GAAGTTGGAT CTCCAGCTGA AGAATTTAAA GCCTTTGCAG CAGACACAGG GATGAACAGA
    AGCCAATCAG AGTACTGCAA CGTGGGCGTC AAGACATACC TGACTAATCA CCCAGCTAAG
    AAATTTGTTT TTGACTTCAT GCGGGTCTTA ATAATAGACA ACCTCTGTCT TACACCTGCC
    AGCAAGCAGA CTCCACTGAT TGATCTTTTG TTGGAGGCTT CTCCTGAGAG ATCGACAAGA
    ACGCAGCAAA AAGAATTTCA GACTTACATT TTGGATAGTG TGATGGATCA TTTGCTTGCA
    GCTGATGTTT TACTGGGAGA AGATGCATCT CTTCCTATCA CCAGTGGAGG AAGCTACCAG
    GTATTGGTGA ACAATGTGTT TTATTTCACA CAACGTGTGG TGGACAAGCT TTGGCAAGGC
    ATGTTCAACA AAGAATCTAA ACTTCTTATA GATTTTATAA TTCAACTAAT TGCACAGTCA
    AAGAGAAGAT CCCAGGGGTT GTCACTGGAT GCAGTCTATC ACTGCCTCAA CAGGACTATC
    CTGTACCAGT TCTCCCGGGC GCACAAAACC GTCCCGCAGC AAGTAGCCCT TCTTGACTCT
    CTCAGGGTCC TGACTGTCAA CAGGAACTTG ATCCTGGGCC CCGGAAACCA CGACCAAGAA
    TTCATCAGCT GTCTGGCTCA CTGCCTCATT AACCTGCATG TTGGGAGCAA TGTGGATGGA
    TTTGGACTGG AAGCAGAAGC CCGCATGACT ACATGGCATA TTATGATCCC CTCGGACATT
    GAACCAGATG GTGGTTACAG CCAAGATATT AGTGAAGGGC GTCAGCTTCT CATAAAAGCT
    GTCAACAGAG TTTGGACGGA ATTAATTCAT AGTAAGAAAC AAGTCTTGGA GGAGCTTTTC
    AAAGTCACTC TACCTGTGAA TGAAAGAGGC CACGTGGACA TAGCTATAGC AAGGCCGCTC
    ATCGAAGAAG CTAGTTTGAA GTGCTGGCAG AATCATCTAG CCCATGAAAA GAAGTGCATA
    AGTCGAGGAG AAGCTTTAAT GCCCACCACA CAATCCAAAT TGTCCCGTGT CAGCAGTGGC
    TTTGGTCTTT CCAAGTTAAC GGGATCAAGA AGGAATCGAA AGGAAAGCGG GCTTCATAAA
    CACAGCCTTT CCACCCAGGA GATTTCTCAG TGGATGTTTA CACACATCGC TGTTGTTCGT
    GACTTGGTAG ACACACAATA TAAGGAATAC CAGGAGCGTC AGCAAAATGC CCTGAAGTAC
    GTGACGGAAG AGTGGTGCCA GATCGAGTAT GAGCTGCTGC GGGAGCGGGG ACTCTGGGGC
    CCCCCCATTG GTTCCCATCT CGACAAGTGG ATGTTGGAGA TGACAGAGGG TCCCTGCAGA
    ATGAGGAAAA AAATGGTGCG AAATGATATG TTTTATAACC ATTACCCTTA CGTGCCAGAA
    ACGGAGCAGG AAACCAACGT GGCGTCTGAG ATCCCAAGTA AACAGCCTGA GACACCTGAT
    GATATCATTC CTCAAAAGAA GCCTGCTCGA TACAGAAGAG CCGTAAGTTA TGACAGTAAA
    GAATACTACA TGCGACTAGC CTCCGGCAAC CCAGCCATTG TGCAGGATGC CATCGTAGAG
    AGCTCTGAAG GAGAAGCTGC TCAGCAAGAA CCAGAACATG GTGAAGACAC GATTGCTAAA
    GTCAAAGGAC TGGTCAAGCC ACCTCTCAAA CGGTCTCGGT CAGCACCGGA CGGAGGCGAT
    GAGGAGACAC AGGAGCAGTT ACAGGACCAG ACAGCTGAGG GCAGCTCGAT AGAAGAGGAG
    GAGAAAACAG ACAACGCCAC CCTGCTGCGC CTGTTGGAGG AGGGCGAAAA GATCCAGCAC
    ATGTACCGCT GTGCTCGAGT CCAGGGCCTT GATACCAGTG AGGGGCTACT CCTCTTTGGT
    AAAGAGCATT TCTATGTGAT TGATGGATTT ACAATGACAG CAACCAGAGA AATAAGAGAT
    ATTGAAACCT TACCTCCAAA TATGCATGAG CCGATTATCC CTAGAGGAGC CAGGCAAGGC
    CCCAGTCAGC TCAAGAGAAC GTGCAGTATC TTTGCATATG AAGATATCAA GGAAGTGCAT
    AAACGGAGAT ATCTCCTGCA GCCTATCGCT GTGGAAGTTT TCTCTGGAGA TGGACGAAAC
    TACCTCCTTG CTTTTCAGAA AGGAATCAGA AACAAAGTTT ATCAAAGGTT TTTGGCTGTA
    GTGCCATCTC TCACTGACAG TTCAGAGTCT GTGTCTGGGC AGCGGCCGAA CACCAGCGTG
    GAGCAGGGAT CTGGGTTGCT TAGCACTCTG GTTGGAGAGA AGTCTGTGAC TCAGAGATGG
    GAGAGAGGTG AAATCAGCAA CTTTCAGTAT TTGATGCACT TGAACACTTT GGCTGGAAGA
    TCGTATAATG ACCTCATGCA ATATCCTGTC TTCCCCTGGA TCCTTGCAGA TTACGACTCG
    GAGGAGGTCG ATCTTACTAA TCCCAAGACA TTTAGAAACC TGGCCAAGCC AATGGGAGCA
    CAGACAGATG AACGATTAGC TCAGTATAAG AAGCGATATA AAGACTGGGA AGATCCTAAT
    GGAGAAACTC CAGCCTACCA CTATGGGACC CACTATTCAT CAGCCATGAT TGTGGCTTCA
    TACCTTGTCA GAATGGAGCC CTTCACACAG ATATTCCTAA GGCTACAGGG AGGCCACTTT
    GACCTGGCTG ACCGGATGTT TCACAGCGTG CGTGAGGCCT GGTACTCAGC GTCAAAGCAC
    AACATGGCAG ATGTAAAGGA ACTCATCCCA GAATTTTTTT ACTTACCAGA GTTCCTGTTC
    AATTCCAACA ACTTTGACCT TGGCTGTAAA CAAAACGGCA CCAAACTTGG AGATGTTATC
    CTTCCACCCT GGGCAAAAGG GGACCCACGA GAATTCATCC GGGTGCACCG TGAGGCTCTG
    GAATGTGACT ACGTGAGTGC CCATCTGCAT GAGTGGATCG ACTTAATCTT CGGCTATAAG
    CAGCAAGGTC CTGCCGCAGT AGAAGCTGTA AACGTCTTCC ATCACCTTTT CTATGAGGGT
    CAAGTGGATA TCTACAACAT AAATGACCCC CTCAAGGAAA CAGCCACCAT AGGGTTCATT
    AATAACTTTG GGCAGATCCC TAAACAGTTA TTTAAAAAAC CTCATCCACC CAAGCGAGTG
    AGGAGTCGAC TCAACGGAGA CAATGCGGGG GCCTCCGTCC CGCCAGGGTC TGCCAGTGAC
    AAGATCTTTT TTCATCATCT GGACAACCTG AGGCCTTCAC TAACACCTGT AAAAGAACTC
    AAAGAGCCTG TGGGACAAAT CGTGTGTACA GACAAAGGCA TTCTTGCGGT AGAACAGAAT
    AAGGTTCTTA TCCCACCAAC CTGGAACAAG ACTTTCGCCT GGGGCTACGC AGACCTCAGC
    TGCAGGCTGG GGACCTATGA GTCAGACAAG GCAGTGACTG TTTATGAATG TTTGTCGGAG
    TGGGGCCAGA TTCTCTGTGC GATCTGCCCC AACCCCAAGC TGGTCATCAC GGGTGGAACA
    AGTACGGTGG TGTGTGTTTG GGAGATGAGT ACCTCGAAAG AAAAGGCTAA GACTCTCACT
    CTCAAACAGG CCTTACTTGG CCACACGGAT ACGGTTACCT GTGCCACCGC GTCGTTAGCC
    TATCACATCA TCGTCAGCGG GTCCCGAGAC CGAACCTGCA TCATCTGGGA CCTGAACAAG
    CTGTCATTCC TAACCCAGCT CCGAGGCCAC CGGGCTCCCG TGTCTGCTCT GTGTATCAAT
    GAATTAACCG GGGACATCGT GTCCTGCGCC GGCACGTACA TCCATGTGTG GAGCATAAAC
    GGGAACCCCG TCGTGAGTGT GAACACGTTC ACAGGCAGGA GCCAGCAGAT CGTCTGTTGC
    TGTGTGTCGG AGATGAACGA GTGGGACACG CAGAACGTCA TCGTCACGGG ACACTCGGAT
    GGCGTGGTCC GGTTTTGGAG AATGGAATTT TTGCAAGTTC CTGAAACACC AGCCCCTGAG
    CCTGCTGAAG TCCTCGAAAT GCAGGAAGAC TGTCCAGAAG CACAAATCGG GCAAGAAGCC
    CAGGACGAGG ACAGTAGCGA TTCCGAGGCC GACGAGCAGA GCGTCAGCCA GGACCCCAAG
    GACACGCCCA GCCAGCCCAG CAGCACCAGC CACCGGCCCC GGGCAGCCTC CTGCAGAGCC
    ACAGCCACCT GGTATAACGA CAGTGGCTCC GACGACTCCA GGCGCTGGTC CGATCAGCTC
    AGCCTCGATG AGAAAGACGG CTTCATATTT GTGAACTATT CAGAGGGCCA GAGCAGACCT
    CATCTTCAGA CTCCCCTTAA CCACCCCCAC CCCAATCCCA TCGAGGCGCG GAATTACAGC
    CGATTGAAGC CTGGGTACCG CTGGGAACGG CAGCTCGTGT TCAGGAGTAA GCTGACCATG
    CACACGGCCT TTGACCGGAA GGACAATGCT CACCCTGCCG AGATCACTGC ATTGGGCGTC
    TCCAAGGACC ACAGCAGGAT CCTCGTGGGC GACAGTCGGG GCCGGGTGTT CAGCTGGTCT
    GTGAGCGACC AGCCCGGCCG CTCTGCCGCT GACCACTGGG TGAAGGACGA AGGAGGGGAC
    AGCTGTTCAG GCTGCTCCGT GCGGTTCTCT CTCACGGAAA GACGGCACCA TTGCAGGAAC
    TGTGGTCAGC TCTTCTGCCA GAAGTGCAGC CGATTTCAGT CAGAAATCAA GCGCTTGAAA
    ATCTCCTCCC CAGTACGTGT CTGTCAGAAC TGTTACTACA ACTTACAGCA TGAGCGGAGT
    TCAGAAGACG GGCCTCGGAA TTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90115
    Clone ID Related Accession (Same CDS sequence) XM_010797980.2 , XM_015471704.1 , XM_015463824.1 , XM_015471703.1
    Accession Version XM_015471703.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WD repeat and FYVE domain-containing protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103983.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGTC GTGCAGAACA GATCCCTCCT TGCCTGACAA GTGAGTCTTC TCTTCCCTCT
    CCTTGGGGGA CTCCAGCTTT GTCCAGGAAA AGGCACGCGT ATCATTCTGT TTCTACTCCC
    CCCGTCTACC CTCCTAAAAA CGTTGCTGAC CTGAAACCAC ACGTGGCAAC TTCATCTCTC
    TCAAGTTCTG ATGCCGTCAT CATCCATCCT GGAGCCATGC TTGCTATGCT GGATCTCCTG
    GCCTCTGTTG GGTCAGTGAC ACAGCCAGAA CATGCCTTGG ACCTTCAACT TGCCGTGGCC
    AATATTTTAC AATCCCTGGT GCACACGGAA AGAAACCAGC AGGTCATGTG TGAAGCTGGT
    CTCCATGCAC GACTGCTGCA GCGGTGCAGT GCCGCTCTGG CTGACGAGGA CCACTCGCTG
    CACCCACCCC TGCAGCGCAT GTTCGAGCGA TTAGCCTCTC AAGCGCTGGA GCCCATGGTG
    CTCAGGGAGT TTTTGCGTTT GGCAAGTCCA TTAAATTGTG GTGCCTGGGA CAAAAAACTG
    CTAAAACAAT ATAGGGTCCA CAAACCAAGT TCACTGAGTT TTGAGCCAGA GATGAGAAGC
    AGTATGATCA CATCCATGGA AGGTCTGGGT TCTGATAATG TTTTCAGCTT ACATGAAGAT
    AACCATTACC GGATAAGCAA GAGCCTAGTG AAATCTGCAG AAGGAAGTAC TGTACCCCTA
    ACCAGAGTGA AATGTCTGGT CTCCATGACA ACCCCACATG ACATCAGACT TCATGGGTCA
    TCAGTTACTC CAGCTTTTGT TGAATTTGAC ACATCCCTTG AAGGGTTTGG CTGCCTGTTC
    CTGCCCAGTC TGGCCCCTCA TAATGCCCCT ACAAACAATG CTGTCACAAC AGGTCTTATC
    GATGGGGCCG TGGTCAGCGG TATTGGTTCT GGTGAACGAT TCTTCCCTCC TCCATCTGGC
    TTAAGCTACT CCAGCTGGTT TTGCATTGAA CATTTTAGTT CTCCTCCCAG TAACCACCCA
    GTCAGACTCC TTACTGTTGT TCGCCGAGCA AATTCTTCTG AGCACCATTA TGTATGCCTT
    GCCATAGTTC TCTCTGCAAA AGATCGGTCT CTGATTGTCT CCACCAAAGA GGAACTACTC
    CAAAATTATG TTGATGATTT TAGTGAAGAA TCCTCTTTCT ATGAGATTCT GCCATGCTGT
    GCTCGCTTCC GTTGTGGAGA ACTGATCGTT GAGGGACAGT GGCATCACCT GGTTCTGGTG
    ATGAGCAAAG GGATGCTGAA GAACAGCACT GCAACCCTCT ACATCGACGG CCAGCTTGTC
    AGCACTGTGA AGCTTCATTA TGTTCACAGT ACTCCCGGGG GCTCAGGCTC TGCAAACCCA
    CCAGTGGTTA GCACAGTCTA TGCCTACATT GGTACTCCGC CTGCCCAGCG CCAAATTGCT
    TCATTGGTTT GGCGCCTGGG ACCTACACAC TTTCTAGAAG AAGTATTACC TCCTTCAAAT
    GTTACTACCA TTTATGAACT AGGACCAAAT TACGTGGGAA GCTTTCAGGC CGTATGTATG
    CCATGTAAAG ATGCAAAATC TGAAGGTGTG GTCCCATCTC CTGTGTCATT AGTACCAGAG
    GAAAAGGTGT CATTCGGCCT CTATGCACTG TCCGTGTCCG CCTTGACAGT GGCGAGAATC
    CGGAAGGTGT ACAACAAGCT GGACAGCAAA GCCATCGCTA AGCAGTTAGG CATTTCCTCC
    CATGAGAACG CCACTCCTGT GAAGCTGATC CACAACTCCG CAGGACACCT CAATGGGCCC
    GCACGGACAA TCGGGGCCAG TCTGATTGGA TACTTGGGAG TAAGAACATT TGTTCCTAAG
    CCTGTTGCCA CTACTTTGCA GTACGTTGGT GGGGCCGCAG CCATCCTGGG CCTGGTAGCC
    ATGGCTTCTG ATGTGGAAGG GCTGTATGCA GCGGTCAAGG CCCTAGTTTG TGTGGTCAAG
    AGTAACCCAC TAGCCAGCAA AGAAATGGAA AGAATCCGGG GCTACCAGTT GCTGGCAATG
    CTGTTTAAGA AGAAACGCTC CCTTCTTAAC AGCCACATTC TCCATCTCAC TTTTTCCTTG
    GTGGGAACTG TTGACAGTGG ACATGAGACT TCCATTATTC CAAATTCAAC AGCTTTCCAG
    GATCTACTTT GTGATTTTGA AGTCTGGCTC CATGCTCCAT ATGAACTTCA TCTCTCCCTA
    TTTGAACACT TTATTGAATT GCTCACAGAG TCCAGTGAAG CTTCAAAGAA TGCCAAGTTG
    ATGAGGGAAT TCCAGCTAAT CCCAAAGCTG CTACTGACTC TTCGAGATAT GTCTCTATCC
    CAGCCTACCA TTGCTGCTAT TAGTAACGTG CTGAGCTTCT TACTGCAGGG TTTTCCCAAC
    AGCAATGATC TACTCAGGTT TGGCCAGTTC ATTTCTTCTA CTTTACCAAC CTTTGCAGTT
    TGTGAGAAGT TTGTAGTTAT GGAAATAAAC CACGAAGAGA AGCCTGACAC TGGAACTGAA
    GAAGAGTTTG GAGGTCTTGT ATCTGCTAAT CTTATACTTT TGAGGAACAG ACTTCTGGAT
    ATCTTACTAA AACTGGTTTA CACATCTAAA GAAAAGACAA GCATTAATTT GCAAGCTTGT
    GAAGAGCTGG TCAGGACGCT GGGCTTTGAC TGGATTATGA TGTTTATGGA AGAACACTTG
    CATTCTACCA CAGTCACAGC AGCCATGAGG ATTCTTGTTG TCCTACTGAG TAATCAGTCT
    ATTCTCATCA AGTTCAAGGA GGGACTCAGT GGTGGAGGCT GGCTGGAGCA GACAGATTCT
    GTCTTAACGA ATAAGATCGG AACTGTATTA GGATTCAATG TGGGCAGGAG TGCTGGCGGA
    CGATCAACTG TCAGGGAGAT TAACCGGGAT GCTTGTCACT TTCCTGGCTT TCCGGTGCTT
    CAGTCATTCC TTCCTAAACA CACTAATGTC CCTGCTCTCT ATTTCCTCCT CATGGCATTG
    TTTCTGCAGC AGCCCGTTAG TGAGCTGCCT GAGAACCTGC AGGTCAGTGT GCCTGTCGTC
    AGCAGCCGAT GTAAGCAGGG TTGCCAGTTT GATTTAGATT CCATCTGGAC CTTTATCTTT
    GGAGTTCCTG CCTCCAGTGG AACCGTGGTC TCATCTATCC ACAATGTGTG CACAGAAGCT
    GCTTTTTTGT TACTGGGCAT GCTCCGAAGC ATGCTGAATT CACCTTGGCA ATCAGAAGAA
    GAGGGGTCTT GGCTCCGGGA GTATCCTGTG ACCCTGATGC AGTTCTTCAG ATACTTGTAT
    CACAATGTCC CGGACCTGGC CTCCATGTGG ATGAACCCTG ATTTCCTGTG TGCACTTGCA
    GCCACGGTCT TCCCCTTCAA CATCCGCCCT TATTCAGAAA TGGTGACTGA TCTCGATGAT
    GAAGTTGGAT CTCCAGCTGA AGAATTTAAA GCCTTTGCAG CAGACACAGG GATGAACAGA
    AGCCAATCAG AGTACTGCAA CGTGGGCGTC AAGACATACC TGACTAATCA CCCAGCTAAG
    AAATTTGTTT TTGACTTCAT GCGGGTCTTA ATAATAGACA ACCTCTGTCT TACACCTGCC
    AGCAAGCAGA CTCCACTGAT TGATCTTTTG TTGGAGGCTT CTCCTGAGAG ATCGACAAGA
    ACGCAGCAAA AAGAATTTCA GACTTACATT TTGGATAGTG TGATGGATCA TTTGCTTGCA
    GCTGATGTTT TACTGGGAGA AGATGCATCT CTTCCTATCA CCAGTGGAGG AAGCTACCAG
    GTATTGGTGA ACAATGTGTT TTATTTCACA CAACGTGTGG TGGACAAGCT TTGGCAAGGC
    ATGTTCAACA AAGAATCTAA ACTTCTTATA GATTTTATAA TTCAACTAAT TGCACAGTCA
    AAGAGAAGAT CCCAGGGGTT GTCACTGGAT GCAGTCTATC ACTGCCTCAA CAGGACTATC
    CTGTACCAGT TCTCCCGGGC GCACAAAACC GTCCCGCAGC AAGTAGCCCT TCTTGACTCT
    CTCAGGGTCC TGACTGTCAA CAGGAACTTG ATCCTGGGCC CCGGAAACCA CGACCAAGAA
    TTCATCAGCT GTCTGGCTCA CTGCCTCATT AACCTGCATG TTGGGAGCAA TGTGGATGGA
    TTTGGACTGG AAGCAGAAGC CCGCATGACT ACATGGCATA TTATGATCCC CTCGGACATT
    GAACCAGATG GTGGTTACAG CCAAGATATT AGTGAAGGGC GTCAGCTTCT CATAAAAGCT
    GTCAACAGAG TTTGGACGGA ATTAATTCAT AGTAAGAAAC AAGTCTTGGA GGAGCTTTTC
    AAAGTCACTC TACCTGTGAA TGAAAGAGGC CACGTGGACA TAGCTATAGC AAGGCCGCTC
    ATCGAAGAAG CTAGTTTGAA GTGCTGGCAG AATCATCTAG CCCATGAAAA GAAGTGCATA
    AGTCGAGGAG AAGCTTTAAT GCCCACCACA CAATCCAAAT TGTCCCGTGT CAGCAGTGGC
    TTTGGTCTTT CCAAGTTAAC GGGATCAAGA AGGAATCGAA AGGAAAGCGG GCTTCATAAA
    CACAGCCTTT CCACCCAGGA GATTTCTCAG TGGATGTTTA CACACATCGC TGTTGTTCGT
    GACTTGGTAG ACACACAATA TAAGGAATAC CAGGAGCGTC AGCAAAATGC CCTGAAGTAC
    GTGACGGAAG AGTGGTGCCA GATCGAGTAT GAGCTGCTGC GGGAGCGGGG ACTCTGGGGC
    CCCCCCATTG GTTCCCATCT CGACAAGTGG ATGTTGGAGA TGACAGAGGG TCCCTGCAGA
    ATGAGGAAAA AAATGGTGCG AAATGATATG TTTTATAACC ATTACCCTTA CGTGCCAGAA
    ACGGAGCAGG AAACCAACGT GGCGTCTGAG ATCCCAAGTA AACAGCCTGA GACACCTGAT
    GATATCATTC CTCAAAAGAA GCCTGCTCGA TACAGAAGAG CCGTAAGTTA TGACAGTAAA
    GAATACTACA TGCGACTAGC CTCCGGCAAC CCAGCCATTG TGCAGGATGC CATCGTAGAG
    AGCTCTGAAG GAGAAGCTGC TCAGCAAGAA CCAGAACATG GTGAAGACAC GATTGCTAAA
    GTCAAAGGAC TGGTCAAGCC ACCTCTCAAA CGGTCTCGGT CAGCACCGGA CGGAGGCGAT
    GAGGAGACAC AGGAGCAGTT ACAGGACCAG ACAGCTGAGG GCAGCTCGAT AGAAGAGGAG
    GAGAAAACAG ACAACGCCAC CCTGCTGCGC CTGTTGGAGG AGGGCGAAAA GATCCAGCAC
    ATGTACCGCT GTGCTCGAGT CCAGGGCCTT GATACCAGTG AGGGGCTACT CCTCTTTGGT
    AAAGAGCATT TCTATGTGAT TGATGGATTT ACAATGACAG CAACCAGAGA AATAAGAGAT
    ATTGAAACCT TACCTCCAAA TATGCATGAG CCGATTATCC CTAGAGGAGC CAGGCAAGGC
    CCCAGTCAGC TCAAGAGAAC GTGCAGTATC TTTGCATATG AAGATATCAA GGAAGTGCAT
    AAACGGAGAT ATCTCCTGCA GCCTATCGCT GTGGAAGTTT TCTCTGGAGA TGGACGAAAC
    TACCTCCTTG CTTTTCAGAA AGGAATCAGA AACAAAGTTT ATCAAAGGTT TTTGGCTGTA
    GTGCCATCTC TCACTGACAG TTCAGAGTCT GTGTCTGGGC AGCGGCCGAA CACCAGCGTG
    GAGCAGGGAT CTGGGTTGCT TAGCACTCTG GTTGGAGAGA AGTCTGTGAC TCAGAGATGG
    GAGAGAGGTG AAATCAGCAA CTTTCAGTAT TTGATGCACT TGAACACTTT GGCTGGAAGA
    TCGTATAATG ACCTCATGCA ATATCCTGTC TTCCCCTGGA TCCTTGCAGA TTACGACTCG
    GAGGAGGTCG ATCTTACTAA TCCCAAGACA TTTAGAAACC TGGCCAAGCC AATGGGAGCA
    CAGACAGATG AACGATTAGC TCAGTATAAG AAGCGATATA AAGACTGGGA AGATCCTAAT
    GGAGAAACTC CAGCCTACCA CTATGGGACC CACTATTCAT CAGCCATGAT TGTGGCTTCA
    TACCTTGTCA GAATGGAGCC CTTCACACAG ATATTCCTAA GGCTACAGGG AGGCCACTTT
    GACCTGGCTG ACCGGATGTT TCACAGCGTG CGTGAGGCCT GGTACTCAGC GTCAAAGCAC
    AACATGGCAG ATGTAAAGGA ACTCATCCCA GAATTTTTTT ACTTACCAGA GTTCCTGTTC
    AATTCCAACA ACTTTGACCT TGGCTGTAAA CAAAACGGCA CCAAACTTGG AGATGTTATC
    CTTCCACCCT GGGCAAAAGG GGACCCACGA GAATTCATCC GGGTGCACCG TGAGGCTCTG
    GAATGTGACT ACGTGAGTGC CCATCTGCAT GAGTGGATCG ACTTAATCTT CGGCTATAAG
    CAGCAAGGTC CTGCCGCAGT AGAAGCTGTA AACGTCTTCC ATCACCTTTT CTATGAGGGT
    CAAGTGGATA TCTACAACAT AAATGACCCC CTCAAGGAAA CAGCCACCAT AGGGTTCATT
    AATAACTTTG GGCAGATCCC TAAACAGTTA TTTAAAAAAC CTCATCCACC CAAGCGAGTG
    AGGAGTCGAC TCAACGGAGA CAATGCGGGG GCCTCCGTCC CGCCAGGGTC TGCCAGTGAC
    AAGATCTTTT TTCATCATCT GGACAACCTG AGGCCTTCAC TAACACCTGT AAAAGAACTC
    AAAGAGCCTG TGGGACAAAT CGTGTGTACA GACAAAGGCA TTCTTGCGGT AGAACAGAAT
    AAGGTTCTTA TCCCACCAAC CTGGAACAAG ACTTTCGCCT GGGGCTACGC AGACCTCAGC
    TGCAGGCTGG GGACCTATGA GTCAGACAAG GCAGTGACTG TTTATGAATG TTTGTCGGAG
    TGGGGCCAGA TTCTCTGTGC GATCTGCCCC AACCCCAAGC TGGTCATCAC GGGTGGAACA
    AGTACGGTGG TGTGTGTTTG GGAGATGAGT ACCTCGAAAG AAAAGGCTAA GACTCTCACT
    CTCAAACAGG CCTTACTTGG CCACACGGAT ACGGTTACCT GTGCCACCGC GTCGTTAGCC
    TATCACATCA TCGTCAGCGG GTCCCGAGAC CGAACCTGCA TCATCTGGGA CCTGAACAAG
    CTGTCATTCC TAACCCAGCT CCGAGGCCAC CGGGCTCCCG TGTCTGCTCT GTGTATCAAT
    GAATTAACCG GGGACATCGT GTCCTGCGCC GGCACGTACA TCCATGTGTG GAGCATAAAC
    GGGAACCCCG TCGTGAGTGT GAACACGTTC ACAGGCAGGA GCCAGCAGAT CGTCTGTTGC
    TGTGTGTCGG AGATGAACGA GTGGGACACG CAGAACGTCA TCGTCACGGG ACACTCGGAT
    GGCGTGGTCC GGTTTTGGAG AATGGAATTT TTGCAAGTTC CTGAAACACC AGCCCCTGAG
    CCTGCTGAAG TCCTCGAAAT GCAGGAAGAC TGTCCAGAAG CACAAATCGG GCAAGAAGCC
    CAGGACGAGG ACAGTAGCGA TTCCGAGGCC GACGAGCAGA GCGTCAGCCA GGACCCCAAG
    GACACGCCCA GCCAGCCCAG CAGCACCAGC CACCGGCCCC GGGCAGCCTC CTGCAGAGCC
    ACAGCCACCT GGTATAACGA CAGTGGCTCC GACGACTCCA GGCGCTGGTC CGATCAGCTC
    AGCCTCGATG AGAAAGACGG CTTCATATTT GTGAACTATT CAGAGGGCCA GAGCAGACCT
    CATCTTCAGA CTCCCCTTAA CCACCCCCAC CCCAATCCCA TCGAGGCGCG GAATTACAGC
    CGATTGAAGC CTGGGTACCG CTGGGAACGG CAGCTCGTGT TCAGGAGTAA GCTGACCATG
    CACACGGCCT TTGACCGGAA GGACAATGCT CACCCTGCCG AGATCACTGC ATTGGGCGTC
    TCCAAGGACC ACAGCAGGAT CCTCGTGGGC GACAGTCGGG GCCGGGTGTT CAGCTGGTCT
    GTGAGCGACC AGCCCGGCCG CTCTGCCGCT GACCACTGGG TGAAGGACGA AGGAGGGGAC
    AGCTGTTCAG GCTGCTCCGT GCGGTTCTCT CTCACGGAAA GACGGCACCA TTGCAGGAAC
    TGTGGTCAGC TCTTCTGCCA GAAGTGCAGC CGATTTCAGT CAGAAATCAA GCGCTTGAAA
    ATCTCCTCCC CAGTACGTGT CTGTCAGAAC TGTTACTACA ACTTACAGCA TGAGCGGAGT
    TCAGAAGACG GGCCTCGGAA TTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327189.1
    CDS998..10864
    Translation

    Target ORF information:

    RefSeq Version XM_015471703.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471703.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGTC GTGCAGAACA GATCCCTCCT TGCCTGACAA GTGAGTCTTC TCTTCCCTCT
    CCTTGGGGGA CTCCAGCTTT GTCCAGGAAA AGGCACGCGT ATCATTCTGT TTCTACTCCC
    CCCGTCTACC CTCCTAAAAA CGTTGCTGAC CTGAAACCAC ACGTGGCAAC TTCATCTCTC
    TCAAGTTCTG ATGCCGTCAT CATCCATCCT GGAGCCATGC TTGCTATGCT GGATCTCCTG
    GCCTCTGTTG GGTCAGTGAC ACAGCCAGAA CATGCCTTGG ACCTTCAACT TGCCGTGGCC
    AATATTTTAC AATCCCTGGT GCACACGGAA AGAAACCAGC AGGTCATGTG TGAAGCTGGT
    CTCCATGCAC GACTGCTGCA GCGGTGCAGT GCCGCTCTGG CTGACGAGGA CCACTCGCTG
    CACCCACCCC TGCAGCGCAT GTTCGAGCGA TTAGCCTCTC AAGCGCTGGA GCCCATGGTG
    CTCAGGGAGT TTTTGCGTTT GGCAAGTCCA TTAAATTGTG GTGCCTGGGA CAAAAAACTG
    CTAAAACAAT ATAGGGTCCA CAAACCAAGT TCACTGAGTT TTGAGCCAGA GATGAGAAGC
    AGTATGATCA CATCCATGGA AGGTCTGGGT TCTGATAATG TTTTCAGCTT ACATGAAGAT
    AACCATTACC GGATAAGCAA GAGCCTAGTG AAATCTGCAG AAGGAAGTAC TGTACCCCTA
    ACCAGAGTGA AATGTCTGGT CTCCATGACA ACCCCACATG ACATCAGACT TCATGGGTCA
    TCAGTTACTC CAGCTTTTGT TGAATTTGAC ACATCCCTTG AAGGGTTTGG CTGCCTGTTC
    CTGCCCAGTC TGGCCCCTCA TAATGCCCCT ACAAACAATG CTGTCACAAC AGGTCTTATC
    GATGGGGCCG TGGTCAGCGG TATTGGTTCT GGTGAACGAT TCTTCCCTCC TCCATCTGGC
    TTAAGCTACT CCAGCTGGTT TTGCATTGAA CATTTTAGTT CTCCTCCCAG TAACCACCCA
    GTCAGACTCC TTACTGTTGT TCGCCGAGCA AATTCTTCTG AGCACCATTA TGTATGCCTT
    GCCATAGTTC TCTCTGCAAA AGATCGGTCT CTGATTGTCT CCACCAAAGA GGAACTACTC
    CAAAATTATG TTGATGATTT TAGTGAAGAA TCCTCTTTCT ATGAGATTCT GCCATGCTGT
    GCTCGCTTCC GTTGTGGAGA ACTGATCGTT GAGGGACAGT GGCATCACCT GGTTCTGGTG
    ATGAGCAAAG GGATGCTGAA GAACAGCACT GCAACCCTCT ACATCGACGG CCAGCTTGTC
    AGCACTGTGA AGCTTCATTA TGTTCACAGT ACTCCCGGGG GCTCAGGCTC TGCAAACCCA
    CCAGTGGTTA GCACAGTCTA TGCCTACATT GGTACTCCGC CTGCCCAGCG CCAAATTGCT
    TCATTGGTTT GGCGCCTGGG ACCTACACAC TTTCTAGAAG AAGTATTACC TCCTTCAAAT
    GTTACTACCA TTTATGAACT AGGACCAAAT TACGTGGGAA GCTTTCAGGC CGTATGTATG
    CCATGTAAAG ATGCAAAATC TGAAGGTGTG GTCCCATCTC CTGTGTCATT AGTACCAGAG
    GAAAAGGTGT CATTCGGCCT CTATGCACTG TCCGTGTCCG CCTTGACAGT GGCGAGAATC
    CGGAAGGTGT ACAACAAGCT GGACAGCAAA GCCATCGCTA AGCAGTTAGG CATTTCCTCC
    CATGAGAACG CCACTCCTGT GAAGCTGATC CACAACTCCG CAGGACACCT CAATGGGCCC
    GCACGGACAA TCGGGGCCAG TCTGATTGGA TACTTGGGAG TAAGAACATT TGTTCCTAAG
    CCTGTTGCCA CTACTTTGCA GTACGTTGGT GGGGCCGCAG CCATCCTGGG CCTGGTAGCC
    ATGGCTTCTG ATGTGGAAGG GCTGTATGCA GCGGTCAAGG CCCTAGTTTG TGTGGTCAAG
    AGTAACCCAC TAGCCAGCAA AGAAATGGAA AGAATCCGGG GCTACCAGTT GCTGGCAATG
    CTGTTTAAGA AGAAACGCTC CCTTCTTAAC AGCCACATTC TCCATCTCAC TTTTTCCTTG
    GTGGGAACTG TTGACAGTGG ACATGAGACT TCCATTATTC CAAATTCAAC AGCTTTCCAG
    GATCTACTTT GTGATTTTGA AGTCTGGCTC CATGCTCCAT ATGAACTTCA TCTCTCCCTA
    TTTGAACACT TTATTGAATT GCTCACAGAG TCCAGTGAAG CTTCAAAGAA TGCCAAGTTG
    ATGAGGGAAT TCCAGCTAAT CCCAAAGCTG CTACTGACTC TTCGAGATAT GTCTCTATCC
    CAGCCTACCA TTGCTGCTAT TAGTAACGTG CTGAGCTTCT TACTGCAGGG TTTTCCCAAC
    AGCAATGATC TACTCAGGTT TGGCCAGTTC ATTTCTTCTA CTTTACCAAC CTTTGCAGTT
    TGTGAGAAGT TTGTAGTTAT GGAAATAAAC CACGAAGAGA AGCCTGACAC TGGAACTGAA
    GAAGAGTTTG GAGGTCTTGT ATCTGCTAAT CTTATACTTT TGAGGAACAG ACTTCTGGAT
    ATCTTACTAA AACTGGTTTA CACATCTAAA GAAAAGACAA GCATTAATTT GCAAGCTTGT
    GAAGAGCTGG TCAGGACGCT GGGCTTTGAC TGGATTATGA TGTTTATGGA AGAACACTTG
    CATTCTACCA CAGTCACAGC AGCCATGAGG ATTCTTGTTG TCCTACTGAG TAATCAGTCT
    ATTCTCATCA AGTTCAAGGA GGGACTCAGT GGTGGAGGCT GGCTGGAGCA GACAGATTCT
    GTCTTAACGA ATAAGATCGG AACTGTATTA GGATTCAATG TGGGCAGGAG TGCTGGCGGA
    CGATCAACTG TCAGGGAGAT TAACCGGGAT GCTTGTCACT TTCCTGGCTT TCCGGTGCTT
    CAGTCATTCC TTCCTAAACA CACTAATGTC CCTGCTCTCT ATTTCCTCCT CATGGCATTG
    TTTCTGCAGC AGCCCGTTAG TGAGCTGCCT GAGAACCTGC AGGTCAGTGT GCCTGTCGTC
    AGCAGCCGAT GTAAGCAGGG TTGCCAGTTT GATTTAGATT CCATCTGGAC CTTTATCTTT
    GGAGTTCCTG CCTCCAGTGG AACCGTGGTC TCATCTATCC ACAATGTGTG CACAGAAGCT
    GCTTTTTTGT TACTGGGCAT GCTCCGAAGC ATGCTGAATT CACCTTGGCA ATCAGAAGAA
    GAGGGGTCTT GGCTCCGGGA GTATCCTGTG ACCCTGATGC AGTTCTTCAG ATACTTGTAT
    CACAATGTCC CGGACCTGGC CTCCATGTGG ATGAACCCTG ATTTCCTGTG TGCACTTGCA
    GCCACGGTCT TCCCCTTCAA CATCCGCCCT TATTCAGAAA TGGTGACTGA TCTCGATGAT
    GAAGTTGGAT CTCCAGCTGA AGAATTTAAA GCCTTTGCAG CAGACACAGG GATGAACAGA
    AGCCAATCAG AGTACTGCAA CGTGGGCGTC AAGACATACC TGACTAATCA CCCAGCTAAG
    AAATTTGTTT TTGACTTCAT GCGGGTCTTA ATAATAGACA ACCTCTGTCT TACACCTGCC
    AGCAAGCAGA CTCCACTGAT TGATCTTTTG TTGGAGGCTT CTCCTGAGAG ATCGACAAGA
    ACGCAGCAAA AAGAATTTCA GACTTACATT TTGGATAGTG TGATGGATCA TTTGCTTGCA
    GCTGATGTTT TACTGGGAGA AGATGCATCT CTTCCTATCA CCAGTGGAGG AAGCTACCAG
    GTATTGGTGA ACAATGTGTT TTATTTCACA CAACGTGTGG TGGACAAGCT TTGGCAAGGC
    ATGTTCAACA AAGAATCTAA ACTTCTTATA GATTTTATAA TTCAACTAAT TGCACAGTCA
    AAGAGAAGAT CCCAGGGGTT GTCACTGGAT GCAGTCTATC ACTGCCTCAA CAGGACTATC
    CTGTACCAGT TCTCCCGGGC GCACAAAACC GTCCCGCAGC AAGTAGCCCT TCTTGACTCT
    CTCAGGGTCC TGACTGTCAA CAGGAACTTG ATCCTGGGCC CCGGAAACCA CGACCAAGAA
    TTCATCAGCT GTCTGGCTCA CTGCCTCATT AACCTGCATG TTGGGAGCAA TGTGGATGGA
    TTTGGACTGG AAGCAGAAGC CCGCATGACT ACATGGCATA TTATGATCCC CTCGGACATT
    GAACCAGATG GTGGTTACAG CCAAGATATT AGTGAAGGGC GTCAGCTTCT CATAAAAGCT
    GTCAACAGAG TTTGGACGGA ATTAATTCAT AGTAAGAAAC AAGTCTTGGA GGAGCTTTTC
    AAAGTCACTC TACCTGTGAA TGAAAGAGGC CACGTGGACA TAGCTATAGC AAGGCCGCTC
    ATCGAAGAAG CTAGTTTGAA GTGCTGGCAG AATCATCTAG CCCATGAAAA GAAGTGCATA
    AGTCGAGGAG AAGCTTTAAT GCCCACCACA CAATCCAAAT TGTCCCGTGT CAGCAGTGGC
    TTTGGTCTTT CCAAGTTAAC GGGATCAAGA AGGAATCGAA AGGAAAGCGG GCTTCATAAA
    CACAGCCTTT CCACCCAGGA GATTTCTCAG TGGATGTTTA CACACATCGC TGTTGTTCGT
    GACTTGGTAG ACACACAATA TAAGGAATAC CAGGAGCGTC AGCAAAATGC CCTGAAGTAC
    GTGACGGAAG AGTGGTGCCA GATCGAGTAT GAGCTGCTGC GGGAGCGGGG ACTCTGGGGC
    CCCCCCATTG GTTCCCATCT CGACAAGTGG ATGTTGGAGA TGACAGAGGG TCCCTGCAGA
    ATGAGGAAAA AAATGGTGCG AAATGATATG TTTTATAACC ATTACCCTTA CGTGCCAGAA
    ACGGAGCAGG AAACCAACGT GGCGTCTGAG ATCCCAAGTA AACAGCCTGA GACACCTGAT
    GATATCATTC CTCAAAAGAA GCCTGCTCGA TACAGAAGAG CCGTAAGTTA TGACAGTAAA
    GAATACTACA TGCGACTAGC CTCCGGCAAC CCAGCCATTG TGCAGGATGC CATCGTAGAG
    AGCTCTGAAG GAGAAGCTGC TCAGCAAGAA CCAGAACATG GTGAAGACAC GATTGCTAAA
    GTCAAAGGAC TGGTCAAGCC ACCTCTCAAA CGGTCTCGGT CAGCACCGGA CGGAGGCGAT
    GAGGAGACAC AGGAGCAGTT ACAGGACCAG ACAGCTGAGG GCAGCTCGAT AGAAGAGGAG
    GAGAAAACAG ACAACGCCAC CCTGCTGCGC CTGTTGGAGG AGGGCGAAAA GATCCAGCAC
    ATGTACCGCT GTGCTCGAGT CCAGGGCCTT GATACCAGTG AGGGGCTACT CCTCTTTGGT
    AAAGAGCATT TCTATGTGAT TGATGGATTT ACAATGACAG CAACCAGAGA AATAAGAGAT
    ATTGAAACCT TACCTCCAAA TATGCATGAG CCGATTATCC CTAGAGGAGC CAGGCAAGGC
    CCCAGTCAGC TCAAGAGAAC GTGCAGTATC TTTGCATATG AAGATATCAA GGAAGTGCAT
    AAACGGAGAT ATCTCCTGCA GCCTATCGCT GTGGAAGTTT TCTCTGGAGA TGGACGAAAC
    TACCTCCTTG CTTTTCAGAA AGGAATCAGA AACAAAGTTT ATCAAAGGTT TTTGGCTGTA
    GTGCCATCTC TCACTGACAG TTCAGAGTCT GTGTCTGGGC AGCGGCCGAA CACCAGCGTG
    GAGCAGGGAT CTGGGTTGCT TAGCACTCTG GTTGGAGAGA AGTCTGTGAC TCAGAGATGG
    GAGAGAGGTG AAATCAGCAA CTTTCAGTAT TTGATGCACT TGAACACTTT GGCTGGAAGA
    TCGTATAATG ACCTCATGCA ATATCCTGTC TTCCCCTGGA TCCTTGCAGA TTACGACTCG
    GAGGAGGTCG ATCTTACTAA TCCCAAGACA TTTAGAAACC TGGCCAAGCC AATGGGAGCA
    CAGACAGATG AACGATTAGC TCAGTATAAG AAGCGATATA AAGACTGGGA AGATCCTAAT
    GGAGAAACTC CAGCCTACCA CTATGGGACC CACTATTCAT CAGCCATGAT TGTGGCTTCA
    TACCTTGTCA GAATGGAGCC CTTCACACAG ATATTCCTAA GGCTACAGGG AGGCCACTTT
    GACCTGGCTG ACCGGATGTT TCACAGCGTG CGTGAGGCCT GGTACTCAGC GTCAAAGCAC
    AACATGGCAG ATGTAAAGGA ACTCATCCCA GAATTTTTTT ACTTACCAGA GTTCCTGTTC
    AATTCCAACA ACTTTGACCT TGGCTGTAAA CAAAACGGCA CCAAACTTGG AGATGTTATC
    CTTCCACCCT GGGCAAAAGG GGACCCACGA GAATTCATCC GGGTGCACCG TGAGGCTCTG
    GAATGTGACT ACGTGAGTGC CCATCTGCAT GAGTGGATCG ACTTAATCTT CGGCTATAAG
    CAGCAAGGTC CTGCCGCAGT AGAAGCTGTA AACGTCTTCC ATCACCTTTT CTATGAGGGT
    CAAGTGGATA TCTACAACAT AAATGACCCC CTCAAGGAAA CAGCCACCAT AGGGTTCATT
    AATAACTTTG GGCAGATCCC TAAACAGTTA TTTAAAAAAC CTCATCCACC CAAGCGAGTG
    AGGAGTCGAC TCAACGGAGA CAATGCGGGG GCCTCCGTCC CGCCAGGGTC TGCCAGTGAC
    AAGATCTTTT TTCATCATCT GGACAACCTG AGGCCTTCAC TAACACCTGT AAAAGAACTC
    AAAGAGCCTG TGGGACAAAT CGTGTGTACA GACAAAGGCA TTCTTGCGGT AGAACAGAAT
    AAGGTTCTTA TCCCACCAAC CTGGAACAAG ACTTTCGCCT GGGGCTACGC AGACCTCAGC
    TGCAGGCTGG GGACCTATGA GTCAGACAAG GCAGTGACTG TTTATGAATG TTTGTCGGAG
    TGGGGCCAGA TTCTCTGTGC GATCTGCCCC AACCCCAAGC TGGTCATCAC GGGTGGAACA
    AGTACGGTGG TGTGTGTTTG GGAGATGAGT ACCTCGAAAG AAAAGGCTAA GACTCTCACT
    CTCAAACAGG CCTTACTTGG CCACACGGAT ACGGTTACCT GTGCCACCGC GTCGTTAGCC
    TATCACATCA TCGTCAGCGG GTCCCGAGAC CGAACCTGCA TCATCTGGGA CCTGAACAAG
    CTGTCATTCC TAACCCAGCT CCGAGGCCAC CGGGCTCCCG TGTCTGCTCT GTGTATCAAT
    GAATTAACCG GGGACATCGT GTCCTGCGCC GGCACGTACA TCCATGTGTG GAGCATAAAC
    GGGAACCCCG TCGTGAGTGT GAACACGTTC ACAGGCAGGA GCCAGCAGAT CGTCTGTTGC
    TGTGTGTCGG AGATGAACGA GTGGGACACG CAGAACGTCA TCGTCACGGG ACACTCGGAT
    GGCGTGGTCC GGTTTTGGAG AATGGAATTT TTGCAAGTTC CTGAAACACC AGCCCCTGAG
    CCTGCTGAAG TCCTCGAAAT GCAGGAAGAC TGTCCAGAAG CACAAATCGG GCAAGAAGCC
    CAGGACGAGG ACAGTAGCGA TTCCGAGGCC GACGAGCAGA GCGTCAGCCA GGACCCCAAG
    GACACGCCCA GCCAGCCCAG CAGCACCAGC CACCGGCCCC GGGCAGCCTC CTGCAGAGCC
    ACAGCCACCT GGTATAACGA CAGTGGCTCC GACGACTCCA GGCGCTGGTC CGATCAGCTC
    AGCCTCGATG AGAAAGACGG CTTCATATTT GTGAACTATT CAGAGGGCCA GAGCAGACCT
    CATCTTCAGA CTCCCCTTAA CCACCCCCAC CCCAATCCCA TCGAGGCGCG GAATTACAGC
    CGATTGAAGC CTGGGTACCG CTGGGAACGG CAGCTCGTGT TCAGGAGTAA GCTGACCATG
    CACACGGCCT TTGACCGGAA GGACAATGCT CACCCTGCCG AGATCACTGC ATTGGGCGTC
    TCCAAGGACC ACAGCAGGAT CCTCGTGGGC GACAGTCGGG GCCGGGTGTT CAGCTGGTCT
    GTGAGCGACC AGCCCGGCCG CTCTGCCGCT GACCACTGGG TGAAGGACGA AGGAGGGGAC
    AGCTGTTCAG GCTGCTCCGT GCGGTTCTCT CTCACGGAAA GACGGCACCA TTGCAGGAAC
    TGTGGTCAGC TCTTCTGCCA GAAGTGCAGC CGATTTCAGT CAGAAATCAA GCGCTTGAAA
    ATCTCCTCCC CAGTACGTGT CTGTCAGAAC TGTTACTACA ACTTACAGCA TGAGCGGAGT
    TCAGAAGACG GGCCTCGGAA TTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90116
    Clone ID Related Accession (Same CDS sequence) XM_015463825.1 , XM_015471705.1
    Accession Version XM_015463825.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WD repeat and FYVE domain-containing protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644958.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGTC GTGCAGAACA GATCCCTCCT TGCCTGACAA GTGAGTCTTC TCTTCCCTCT
    CCTTGGGGGA CTCCAGCTTT GTCCAGGAAA AGGCACGCGT ATCATTCTGT TTCTACTCCC
    CCCGTCTACC CTCCTAAAAA CGTTGCTGAC CTGAAACCAC ACGTGGCAAC TTCATCTCTC
    TCAAGTTCTG ATGCCGTCAT CATCCATCCT GGAGCCATGC TTGCTATGCT GGATCTCCTG
    GCCTCTGTTG GGTCAGTGAC ACAGCCAGAA CATGCCTTGG ACCTTCAACT TGCCGTGGCC
    AATATTTTAC AATCCCTGGT GCACACGGAA AGAAACCAGC AGGTCATGTG TGAAGCTGGT
    CTCCATGCAC GACTGCTGCA GCGGTGCAGT GCCGCTCTGG CTGACGAGGA CCACTCGCTG
    CACCCACCCC TGCAGCGCAT GTTCGAGCGA TTAGCCTCTC AAGCGCTGGA GCCCATGGTG
    CTCAGGGAGT TTTTGCGTTT GGCAAGTCCA TTAAATTGTG GTGCCTGGGA CAAAAAACTG
    CTAAAACAAT ATAGGGTCCA CAAACCAAGT TCACTGAGTT TTGAGCCAGA GATGAGAAGC
    AGTATGATCA CATCCATGGA AGGTCTGGGT TCTGATAATG TTTTCAGCTT ACATGAAGAT
    AACCATTACC GGATAAGCAA GAGCCTAGTG AAATCTGCAG AAGGAAGTAC TGTACCCCTA
    ACCAGAGTGA AATGTCTGGT CTCCATGACA ACCCCACATG ACATCAGACT TCATGGGTCA
    TCAGTTACTC CAGCTTTTGT TGAATTTGAC ACATCCCTTG AAGGGTTTGG CTGCCTGTTC
    CTGCCCAGTC TGGCCCCTCA TAATGCCCCT ACAAACAATG CTGTCACAAC AGGTCTTATC
    GATGGGGCCG TGGTCAGCGG TATTGGTTCT GGTGAACGAT TCTTCCCTCC TCCATCTGGC
    TTAAGCTACT CCAGCTGGTT TTGCATTGAA CATTTTAGTT CTCCTCCCAG TAACCACCCA
    GTCAGACTCC TTACTGTTGT TCGCCGAGCA AATTCTTCTG AGCACCATTA TGTATGCCTT
    GCCATAGTTC TCTCTGCAAA AGATCGGTCT CTGATTGTCT CCACCAAAGA GGAACTACTC
    CAAAATTATG TTGATGATTT TAGTGAAGAA TCCTCTTTCT ATGAGATTCT GCCATGCTGT
    GCTCGCTTCC GTTGTGGAGA ACTGATCGTT GAGGGACAGT GGCATCACCT GGTTCTGGTG
    ATGAGCAAAG GGATGCTGAA GAACAGCACT GCAACCCTCT ACATCGACGG CCAGCTTGTC
    AGCACTGTGA AGCTTCATTA TGTTCACAGT ACTCCCGGGG GCTCAGGCTC TGCAAACCCA
    CCAGTGGTTA GCACAGTCTA TGCCTACATT GGTACTCCGC CTGCCCAGCG CCAAATTGCT
    TCATTGGTTT GGCGCCTGGG ACCTACACAC TTTCTAGAAG AAGTATTACC TCCTTCAAAT
    GTTACTACCA TTTATGAACT AGGACCAAAT TACGTGGGAA GCTTTCAGGC CGTATGTATG
    CCATGTAAAG ATGCAAAATC TGAAGGTGTG GTCCCATCTC CTGTGTCATT AGTACCAGAG
    GAAAAGGTGT CATTCGGCCT CTATGCACTG TCCGTGTCCG CCTTGACAGT GGCGAGAATC
    CGGAAGGTGT ACAACAAGCT GGACAGCAAA GCCATCGCTA AGCAGTTAGG CATTTCCTCC
    CATGAGAACG CCACTCCTGT GAAGCTGATC CACAACTCCG CAGGACACCT CAATGGGCCC
    GCACGGACAA TCGGGGCCAG TCTGATTGGA TACTTGGGAG TAAGAACATT TGTTCCTAAG
    CCTGTTGCCA CTACTTTGCA GTACGTTGGT GGGGCCGCAG CCATCCTGGG CCTGGTAGCC
    ATGGCTTCTG ATGTGGAAGG GCTGTATGCA GCGGTCAAGG CCCTAGTTTG TGTGGTCAAG
    AGTAACCCAC TAGCCAGCAA AGAAATGGAA AGAATCCGGG GCTACCAGTT GCTGGCAATG
    CTGTTTAAGA AGAAACGCTC CCTTCTTAAC AGCCACATTC TCCATCTCAC TTTTTCCTTG
    GTGGGAACTG TTGACAGTGG ACATGAGACT TCCATTATTC CAAATTCAAC AGCTTTCCAG
    GATCTACTTT GTGATTTTGA AGTCTGGCTC CATGCTCCAT ATGAACTTCA TCTCTCCCTA
    TTTGAACACT TTATTGAATT GCTCACAGAG TCCAGTGAAG CTTCAAAGAA TGCCAAGTTG
    ATGAGGGAAT TCCAGCTAAT CCCAAAGCTG CTACTGACTC TTCGAGATAT GTCTCTATCC
    CAGCCTACCA TTGCTGCTAT TAGTAACGTG CTGAGCTTCT TACTGCAGGG TTTTCCCAAC
    AGCAATGATC TACTCAGGTT TGGCCAGTTC ATTTCTTCTA CTTTACCAAC CTTTGCAGTT
    TGTGAGAAGT TTGTAGTTAT GGAAATAAAC CACGAAGAGA AGCCTGACAC TGGAACTGAA
    GAAGAGTTTG GAGGTCTTGT ATCTGCTAAT CTTATACTTT TGAGGAACAG ACTTCTGGAT
    ATCTTACTAA AACTGGTTTA CACATCTAAA GAAAAGACAA GCATTAATTT GCAAGCTTGT
    GAAGAGCTGG TCAGGACGCT GGGCTTTGAC TGGATTATGA TGTTTATGGA AGAACACTTG
    CATTCTACCA CAGTCACAGC AGCCATGAGG ATTCTTGTTG TCCTACTGAG TAATCAGTCT
    ATTCTCATCA AGTTCAAGGA GGGACTCAGT GGTGGAGGCT GGCTGGAGCA GACAGATTCT
    GTCTTAACGA ATAAGATCGG AACTGTATTA GGATTCAATG TGGGCAGGAG TGCTGGCGGA
    CGATCAACTG TCAGGGAGAT TAACCGGGAT GCTTGTCACT TTCCTGGCTT TCCGGTGCTT
    CAGTCATTCC TTCCTAAACA CACTAATGTC CCTGCTCTCT ATTTCCTCCT CATGGCATTG
    TTTCTGCAGC AGCCCGTTAG TGAGCTGCCT GAGAACCTGC AGTTTGATTT AGATTCCATC
    TGGACCTTTA TCTTTGGAGT TCCTGCCTCC AGTGGAACCG TGGTCTCATC TATCCACAAT
    GTGTGCACAG AAGCTGCTTT TTTGTTACTG GGCATGCTCC GAAGCATGCT GAATTCACCT
    TGGCAATCAG AAGAAGAGGG GTCTTGGCTC CGGGAGTATC CTGTGACCCT GATGCAGTTC
    TTCAGATACT TGTATCACAA TGTCCCGGAC CTGGCCTCCA TGTGGATGAA CCCTGATTTC
    CTGTGTGCAC TTGCAGCCAC GGTCTTCCCC TTCAACATCC GCCCTTATTC AGAAATGGTG
    ACTGATCTCG ATGATGAAGT TGGATCTCCA GCTGAAGAAT TTAAAGCCTT TGCAGCAGAC
    ACAGGGATGA ACAGAAGCCA ATCAGAGTAC TGCAACGTGG GCGTCAAGAC ATACCTGACT
    AATCACCCAG CTAAGAAATT TGTTTTTGAC TTCATGCGGG TCTTAATAAT AGACAACCTC
    TGTCTTACAC CTGCCAGCAA GCAGACTCCA CTGATTGATC TTTTGTTGGA GGCTTCTCCT
    GAGAGATCGA CAAGAACGCA GCAAAAAGAA TTTCAGACTT ACATTTTGGA TAGTGTGATG
    GATCATTTGC TTGCAGCTGA TGTTTTACTG GGAGAAGATG CATCTCTTCC TATCACCAGT
    GGAGGAAGCT ACCAGGTATT GGTGAACAAT GTGTTTTATT TCACACAACG TGTGGTGGAC
    AAGCTTTGGC AAGGCATGTT CAACAAAGAA TCTAAACTTC TTATAGATTT TATAATTCAA
    CTAATTGCAC AGTCAAAGAG AAGATCCCAG GGGTTGTCAC TGGATGCAGT CTATCACTGC
    CTCAACAGGA CTATCCTGTA CCAGTTCTCC CGGGCGCACA AAACCGTCCC GCAGCAAGTA
    GCCCTTCTTG ACTCTCTCAG GGTCCTGACT GTCAACAGGA ACTTGATCCT GGGCCCCGGA
    AACCACGACC AAGAATTCAT CAGCTGTCTG GCTCACTGCC TCATTAACCT GCATGTTGGG
    AGCAATGTGG ATGGATTTGG ACTGGAAGCA GAAGCCCGCA TGACTACATG GCATATTATG
    ATCCCCTCGG ACATTGAACC AGATGGTGGT TACAGCCAAG ATATTAGTGA AGGGCGTCAG
    CTTCTCATAA AAGCTGTCAA CAGAGTTTGG ACGGAATTAA TTCATAGTAA GAAACAAGTC
    TTGGAGGAGC TTTTCAAAGT CACTCTACCT GTGAATGAAA GAGGCCACGT GGACATAGCT
    ATAGCAAGGC CGCTCATCGA AGAAGCTAGT TTGAAGTGCT GGCAGAATCA TCTAGCCCAT
    GAAAAGAAGT GCATAAGTCG AGGAGAAGCT TTAATGCCCA CCACACAATC CAAATTGTCC
    CGTGTCAGCA GTGGCTTTGG TCTTTCCAAG TTAACGGGAT CAAGAAGGAA TCGAAAGGAA
    AGCGGGCTTC ATAAACACAG CCTTTCCACC CAGGAGATTT CTCAGTGGAT GTTTACACAC
    ATCGCTGTTG TTCGTGACTT GGTAGACACA CAATATAAGG AATACCAGGA GCGTCAGCAA
    AATGCCCTGA AGTACGTGAC GGAAGAGTGG TGCCAGATCG AGTATGAGCT GCTGCGGGAG
    CGGGGACTCT GGGGCCCCCC CATTGGTTCC CATCTCGACA AGTGGATGTT GGAGATGACA
    GAGGGTCCCT GCAGAATGAG GAAAAAAATG GTGCGAAATG ATATGTTTTA TAACCATTAC
    CCTTACGTGC CAGAAACGGA GCAGGAAACC AACGTGGCGT CTGAGATCCC AAGTAAACAG
    CCTGAGACAC CTGATGATAT CATTCCTCAA AAGAAGCCTG CTCGATACAG AAGAGCCGTA
    AGTTATGACA GTAAAGAATA CTACATGCGA CTAGCCTCCG GCAACCCAGC CATTGTGCAG
    GATGCCATCG TAGAGAGCTC TGAAGGAGAA GCTGCTCAGC AAGAACCAGA ACATGGTGAA
    GACACGATTG CTAAAGTCAA AGGACTGGTC AAGCCACCTC TCAAACGGTC TCGGTCAGCA
    CCGGACGGAG GCGATGAGGA GACACAGGAG CAGTTACAGG ACCAGACAGC TGAGGGCAGC
    TCGATAGAAG AGGAGGAGAA AACAGACAAC GCCACCCTGC TGCGCCTGTT GGAGGAGGGC
    GAAAAGATCC AGCACATGTA CCGCTGTGCT CGAGTCCAGG GCCTTGATAC CAGTGAGGGG
    CTACTCCTCT TTGGTAAAGA GCATTTCTAT GTGATTGATG GATTTACAAT GACAGCAACC
    AGAGAAATAA GAGATATTGA AACCTTACCT CCAAATATGC ATGAGCCGAT TATCCCTAGA
    GGAGCCAGGC AAGGCCCCAG TCAGCTCAAG AGAACGTGCA GTATCTTTGC ATATGAAGAT
    ATCAAGGAAG TGCATAAACG GAGATATCTC CTGCAGCCTA TCGCTGTGGA AGTTTTCTCT
    GGAGATGGAC GAAACTACCT CCTTGCTTTT CAGAAAGGAA TCAGAAACAA AGTTTATCAA
    AGGTTTTTGG CTGTAGTGCC ATCTCTCACT GACAGTTCAG AGTCTGTGTC TGGGCAGCGG
    CCGAACACCA GCGTGGAGCA GGGATCTGGG TTGCTTAGCA CTCTGGTTGG AGAGAAGTCT
    GTGACTCAGA GATGGGAGAG AGGTGAAATC AGCAACTTTC AGTATTTGAT GCACTTGAAC
    ACTTTGGCTG GAAGATCGTA TAATGACCTC ATGCAATATC CTGTCTTCCC CTGGATCCTT
    GCAGATTACG ACTCGGAGGA GGTCGATCTT ACTAATCCCA AGACATTTAG AAACCTGGCC
    AAGCCAATGG GAGCACAGAC AGATGAACGA TTAGCTCAGT ATAAGAAGCG ATATAAAGAC
    TGGGAAGATC CTAATGGAGA AACTCCAGCC TACCACTATG GGACCCACTA TTCATCAGCC
    ATGATTGTGG CTTCATACCT TGTCAGAATG GAGCCCTTCA CACAGATATT CCTAAGGCTA
    CAGGGAGGCC ACTTTGACCT GGCTGACCGG ATGTTTCACA GCGTGCGTGA GGCCTGGTAC
    TCAGCGTCAA AGCACAACAT GGCAGATGTA AAGGAACTCA TCCCAGAATT TTTTTACTTA
    CCAGAGTTCC TGTTCAATTC CAACAACTTT GACCTTGGCT GTAAACAAAA CGGCACCAAA
    CTTGGAGATG TTATCCTTCC ACCCTGGGCA AAAGGGGACC CACGAGAATT CATCCGGGTG
    CACCGTGAGG CTCTGGAATG TGACTACGTG AGTGCCCATC TGCATGAGTG GATCGACTTA
    ATCTTCGGCT ATAAGCAGCA AGGTCCTGCC GCAGTAGAAG CTGTAAACGT CTTCCATCAC
    CTTTTCTATG AGGGTCAAGT GGATATCTAC AACATAAATG ACCCCCTCAA GGAAACAGCC
    ACCATAGGGT TCATTAATAA CTTTGGGCAG ATCCCTAAAC AGTTATTTAA AAAACCTCAT
    CCACCCAAGC GAGTGAGGAG TCGACTCAAC GGAGACAATG CGGGGGCCTC CGTCCCGCCA
    GGGTCTGCCA GTGACAAGAT CTTTTTTCAT CATCTGGACA ACCTGAGGCC TTCACTAACA
    CCTGTAAAAG AACTCAAAGA GCCTGTGGGA CAAATCGTGT GTACAGACAA AGGCATTCTT
    GCGGTAGAAC AGAATAAGGT TCTTATCCCA CCAACCTGGA ACAAGACTTT CGCCTGGGGC
    TACGCAGACC TCAGCTGCAG GCTGGGGACC TATGAGTCAG ACAAGGCAGT GACTGTTTAT
    GAATGTTTGT CGGAGTGGGG CCAGATTCTC TGTGCGATCT GCCCCAACCC CAAGCTGGTC
    ATCACGGGTG GAACAAGTAC GGTGGTGTGT GTTTGGGAGA TGAGTACCTC GAAAGAAAAG
    GCTAAGACTC TCACTCTCAA ACAGGCCTTA CTTGGCCACA CGGATACGGT TACCTGTGCC
    ACCGCGTCGT TAGCCTATCA CATCATCGTC AGCGGGTCCC GAGACCGAAC CTGCATCATC
    TGGGACCTGA ACAAGCTGTC ATTCCTAACC CAGCTCCGAG GCCACCGGGC TCCCGTGTCT
    GCTCTGTGTA TCAATGAATT AACCGGGGAC ATCGTGTCCT GCGCCGGCAC GTACATCCAT
    GTGTGGAGCA TAAACGGGAA CCCCGTCGTG AGTGTGAACA CGTTCACAGG CAGGAGCCAG
    CAGATCGTCT GTTGCTGTGT GTCGGAGATG AACGAGTGGG ACACGCAGAA CGTCATCGTC
    ACGGGACACT CGGATGGCGT GGTCCGGTTT TGGAGAATGG AATTTTTGCA AGTTCCTGAA
    ACACCAGCCC CTGAGCCTGC TGAAGTCCTC GAAATGCAGG AAGACTGTCC AGAAGCACAA
    ATCGGGCAAG AAGCCCAGGA CGAGGACAGT AGCGATTCCG AGGCCGACGA GCAGAGCGTC
    AGCCAGGACC CCAAGGACAC GCCCAGCCAG CCCAGCAGCA CCAGCCACCG GCCCCGGGCA
    GCCTCCTGCA GAGCCACAGC CACCTGGTAT AACGACAGTG GCTCCGACGA CTCCAGGCGC
    TGGTCCGATC AGCTCAGCCT CGATGAGAAA GACGGCTTCA TATTTGTGAA CTATTCAGAG
    GGCCAGAGCA GACCTCATCT TCAGACTCCC CTTAACCACC CCCACCCCAA TCCCATCGAG
    GCGCGGAATT ACAGCCGATT GAAGCCTGGG TACCGCTGGG AACGGCAGCT CGTGTTCAGG
    AGTAAGCTGA CCATGCACAC GGCCTTTGAC CGGAAGGACA ATGCTCACCC TGCCGAGATC
    ACTGCATTGG GCGTCTCCAA GGACCACAGC AGGATCCTCG TGGGCGACAG TCGGGGCCGG
    GTGTTCAGCT GGTCTGTGAG CGACCAGCCC GGCCGCTCTG CCGCTGACCA CTGGGTGAAG
    GACGAAGGAG GGGACAGCTG TTCAGGCTGC TCCGTGCGGT TCTCTCTCAC GGAAAGACGG
    CACCATTGCA GGAACTGTGG TCAGCTCTTC TGCCAGAAGT GCAGCCGATT TCAGTCAGAA
    ATCAAGCGCT TGAAAATCTC CTCCCCAGTA CGTGTCTGTC AGAACTGTTA CTACAACTTA
    CAGCATGAGC GGAGTTCAGA AGACGGGCCT CGGAATTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319311.1
    CDS998..10819
    Translation

    Target ORF information:

    RefSeq Version XM_015463825.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463825.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGTC GTGCAGAACA GATCCCTCCT TGCCTGACAA GTGAGTCTTC TCTTCCCTCT
    CCTTGGGGGA CTCCAGCTTT GTCCAGGAAA AGGCACGCGT ATCATTCTGT TTCTACTCCC
    CCCGTCTACC CTCCTAAAAA CGTTGCTGAC CTGAAACCAC ACGTGGCAAC TTCATCTCTC
    TCAAGTTCTG ATGCCGTCAT CATCCATCCT GGAGCCATGC TTGCTATGCT GGATCTCCTG
    GCCTCTGTTG GGTCAGTGAC ACAGCCAGAA CATGCCTTGG ACCTTCAACT TGCCGTGGCC
    AATATTTTAC AATCCCTGGT GCACACGGAA AGAAACCAGC AGGTCATGTG TGAAGCTGGT
    CTCCATGCAC GACTGCTGCA GCGGTGCAGT GCCGCTCTGG CTGACGAGGA CCACTCGCTG
    CACCCACCCC TGCAGCGCAT GTTCGAGCGA TTAGCCTCTC AAGCGCTGGA GCCCATGGTG
    CTCAGGGAGT TTTTGCGTTT GGCAAGTCCA TTAAATTGTG GTGCCTGGGA CAAAAAACTG
    CTAAAACAAT ATAGGGTCCA CAAACCAAGT TCACTGAGTT TTGAGCCAGA GATGAGAAGC
    AGTATGATCA CATCCATGGA AGGTCTGGGT TCTGATAATG TTTTCAGCTT ACATGAAGAT
    AACCATTACC GGATAAGCAA GAGCCTAGTG AAATCTGCAG AAGGAAGTAC TGTACCCCTA
    ACCAGAGTGA AATGTCTGGT CTCCATGACA ACCCCACATG ACATCAGACT TCATGGGTCA
    TCAGTTACTC CAGCTTTTGT TGAATTTGAC ACATCCCTTG AAGGGTTTGG CTGCCTGTTC
    CTGCCCAGTC TGGCCCCTCA TAATGCCCCT ACAAACAATG CTGTCACAAC AGGTCTTATC
    GATGGGGCCG TGGTCAGCGG TATTGGTTCT GGTGAACGAT TCTTCCCTCC TCCATCTGGC
    TTAAGCTACT CCAGCTGGTT TTGCATTGAA CATTTTAGTT CTCCTCCCAG TAACCACCCA
    GTCAGACTCC TTACTGTTGT TCGCCGAGCA AATTCTTCTG AGCACCATTA TGTATGCCTT
    GCCATAGTTC TCTCTGCAAA AGATCGGTCT CTGATTGTCT CCACCAAAGA GGAACTACTC
    CAAAATTATG TTGATGATTT TAGTGAAGAA TCCTCTTTCT ATGAGATTCT GCCATGCTGT
    GCTCGCTTCC GTTGTGGAGA ACTGATCGTT GAGGGACAGT GGCATCACCT GGTTCTGGTG
    ATGAGCAAAG GGATGCTGAA GAACAGCACT GCAACCCTCT ACATCGACGG CCAGCTTGTC
    AGCACTGTGA AGCTTCATTA TGTTCACAGT ACTCCCGGGG GCTCAGGCTC TGCAAACCCA
    CCAGTGGTTA GCACAGTCTA TGCCTACATT GGTACTCCGC CTGCCCAGCG CCAAATTGCT
    TCATTGGTTT GGCGCCTGGG ACCTACACAC TTTCTAGAAG AAGTATTACC TCCTTCAAAT
    GTTACTACCA TTTATGAACT AGGACCAAAT TACGTGGGAA GCTTTCAGGC CGTATGTATG
    CCATGTAAAG ATGCAAAATC TGAAGGTGTG GTCCCATCTC CTGTGTCATT AGTACCAGAG
    GAAAAGGTGT CATTCGGCCT CTATGCACTG TCCGTGTCCG CCTTGACAGT GGCGAGAATC
    CGGAAGGTGT ACAACAAGCT GGACAGCAAA GCCATCGCTA AGCAGTTAGG CATTTCCTCC
    CATGAGAACG CCACTCCTGT GAAGCTGATC CACAACTCCG CAGGACACCT CAATGGGCCC
    GCACGGACAA TCGGGGCCAG TCTGATTGGA TACTTGGGAG TAAGAACATT TGTTCCTAAG
    CCTGTTGCCA CTACTTTGCA GTACGTTGGT GGGGCCGCAG CCATCCTGGG CCTGGTAGCC
    ATGGCTTCTG ATGTGGAAGG GCTGTATGCA GCGGTCAAGG CCCTAGTTTG TGTGGTCAAG
    AGTAACCCAC TAGCCAGCAA AGAAATGGAA AGAATCCGGG GCTACCAGTT GCTGGCAATG
    CTGTTTAAGA AGAAACGCTC CCTTCTTAAC AGCCACATTC TCCATCTCAC TTTTTCCTTG
    GTGGGAACTG TTGACAGTGG ACATGAGACT TCCATTATTC CAAATTCAAC AGCTTTCCAG
    GATCTACTTT GTGATTTTGA AGTCTGGCTC CATGCTCCAT ATGAACTTCA TCTCTCCCTA
    TTTGAACACT TTATTGAATT GCTCACAGAG TCCAGTGAAG CTTCAAAGAA TGCCAAGTTG
    ATGAGGGAAT TCCAGCTAAT CCCAAAGCTG CTACTGACTC TTCGAGATAT GTCTCTATCC
    CAGCCTACCA TTGCTGCTAT TAGTAACGTG CTGAGCTTCT TACTGCAGGG TTTTCCCAAC
    AGCAATGATC TACTCAGGTT TGGCCAGTTC ATTTCTTCTA CTTTACCAAC CTTTGCAGTT
    TGTGAGAAGT TTGTAGTTAT GGAAATAAAC CACGAAGAGA AGCCTGACAC TGGAACTGAA
    GAAGAGTTTG GAGGTCTTGT ATCTGCTAAT CTTATACTTT TGAGGAACAG ACTTCTGGAT
    ATCTTACTAA AACTGGTTTA CACATCTAAA GAAAAGACAA GCATTAATTT GCAAGCTTGT
    GAAGAGCTGG TCAGGACGCT GGGCTTTGAC TGGATTATGA TGTTTATGGA AGAACACTTG
    CATTCTACCA CAGTCACAGC AGCCATGAGG ATTCTTGTTG TCCTACTGAG TAATCAGTCT
    ATTCTCATCA AGTTCAAGGA GGGACTCAGT GGTGGAGGCT GGCTGGAGCA GACAGATTCT
    GTCTTAACGA ATAAGATCGG AACTGTATTA GGATTCAATG TGGGCAGGAG TGCTGGCGGA
    CGATCAACTG TCAGGGAGAT TAACCGGGAT GCTTGTCACT TTCCTGGCTT TCCGGTGCTT
    CAGTCATTCC TTCCTAAACA CACTAATGTC CCTGCTCTCT ATTTCCTCCT CATGGCATTG
    TTTCTGCAGC AGCCCGTTAG TGAGCTGCCT GAGAACCTGC AGTTTGATTT AGATTCCATC
    TGGACCTTTA TCTTTGGAGT TCCTGCCTCC AGTGGAACCG TGGTCTCATC TATCCACAAT
    GTGTGCACAG AAGCTGCTTT TTTGTTACTG GGCATGCTCC GAAGCATGCT GAATTCACCT
    TGGCAATCAG AAGAAGAGGG GTCTTGGCTC CGGGAGTATC CTGTGACCCT GATGCAGTTC
    TTCAGATACT TGTATCACAA TGTCCCGGAC CTGGCCTCCA TGTGGATGAA CCCTGATTTC
    CTGTGTGCAC TTGCAGCCAC GGTCTTCCCC TTCAACATCC GCCCTTATTC AGAAATGGTG
    ACTGATCTCG ATGATGAAGT TGGATCTCCA GCTGAAGAAT TTAAAGCCTT TGCAGCAGAC
    ACAGGGATGA ACAGAAGCCA ATCAGAGTAC TGCAACGTGG GCGTCAAGAC ATACCTGACT
    AATCACCCAG CTAAGAAATT TGTTTTTGAC TTCATGCGGG TCTTAATAAT AGACAACCTC
    TGTCTTACAC CTGCCAGCAA GCAGACTCCA CTGATTGATC TTTTGTTGGA GGCTTCTCCT
    GAGAGATCGA CAAGAACGCA GCAAAAAGAA TTTCAGACTT ACATTTTGGA TAGTGTGATG
    GATCATTTGC TTGCAGCTGA TGTTTTACTG GGAGAAGATG CATCTCTTCC TATCACCAGT
    GGAGGAAGCT ACCAGGTATT GGTGAACAAT GTGTTTTATT TCACACAACG TGTGGTGGAC
    AAGCTTTGGC AAGGCATGTT CAACAAAGAA TCTAAACTTC TTATAGATTT TATAATTCAA
    CTAATTGCAC AGTCAAAGAG AAGATCCCAG GGGTTGTCAC TGGATGCAGT CTATCACTGC
    CTCAACAGGA CTATCCTGTA CCAGTTCTCC CGGGCGCACA AAACCGTCCC GCAGCAAGTA
    GCCCTTCTTG ACTCTCTCAG GGTCCTGACT GTCAACAGGA ACTTGATCCT GGGCCCCGGA
    AACCACGACC AAGAATTCAT CAGCTGTCTG GCTCACTGCC TCATTAACCT GCATGTTGGG
    AGCAATGTGG ATGGATTTGG ACTGGAAGCA GAAGCCCGCA TGACTACATG GCATATTATG
    ATCCCCTCGG ACATTGAACC AGATGGTGGT TACAGCCAAG ATATTAGTGA AGGGCGTCAG
    CTTCTCATAA AAGCTGTCAA CAGAGTTTGG ACGGAATTAA TTCATAGTAA GAAACAAGTC
    TTGGAGGAGC TTTTCAAAGT CACTCTACCT GTGAATGAAA GAGGCCACGT GGACATAGCT
    ATAGCAAGGC CGCTCATCGA AGAAGCTAGT TTGAAGTGCT GGCAGAATCA TCTAGCCCAT
    GAAAAGAAGT GCATAAGTCG AGGAGAAGCT TTAATGCCCA CCACACAATC CAAATTGTCC
    CGTGTCAGCA GTGGCTTTGG TCTTTCCAAG TTAACGGGAT CAAGAAGGAA TCGAAAGGAA
    AGCGGGCTTC ATAAACACAG CCTTTCCACC CAGGAGATTT CTCAGTGGAT GTTTACACAC
    ATCGCTGTTG TTCGTGACTT GGTAGACACA CAATATAAGG AATACCAGGA GCGTCAGCAA
    AATGCCCTGA AGTACGTGAC GGAAGAGTGG TGCCAGATCG AGTATGAGCT GCTGCGGGAG
    CGGGGACTCT GGGGCCCCCC CATTGGTTCC CATCTCGACA AGTGGATGTT GGAGATGACA
    GAGGGTCCCT GCAGAATGAG GAAAAAAATG GTGCGAAATG ATATGTTTTA TAACCATTAC
    CCTTACGTGC CAGAAACGGA GCAGGAAACC AACGTGGCGT CTGAGATCCC AAGTAAACAG
    CCTGAGACAC CTGATGATAT CATTCCTCAA AAGAAGCCTG CTCGATACAG AAGAGCCGTA
    AGTTATGACA GTAAAGAATA CTACATGCGA CTAGCCTCCG GCAACCCAGC CATTGTGCAG
    GATGCCATCG TAGAGAGCTC TGAAGGAGAA GCTGCTCAGC AAGAACCAGA ACATGGTGAA
    GACACGATTG CTAAAGTCAA AGGACTGGTC AAGCCACCTC TCAAACGGTC TCGGTCAGCA
    CCGGACGGAG GCGATGAGGA GACACAGGAG CAGTTACAGG ACCAGACAGC TGAGGGCAGC
    TCGATAGAAG AGGAGGAGAA AACAGACAAC GCCACCCTGC TGCGCCTGTT GGAGGAGGGC
    GAAAAGATCC AGCACATGTA CCGCTGTGCT CGAGTCCAGG GCCTTGATAC CAGTGAGGGG
    CTACTCCTCT TTGGTAAAGA GCATTTCTAT GTGATTGATG GATTTACAAT GACAGCAACC
    AGAGAAATAA GAGATATTGA AACCTTACCT CCAAATATGC ATGAGCCGAT TATCCCTAGA
    GGAGCCAGGC AAGGCCCCAG TCAGCTCAAG AGAACGTGCA GTATCTTTGC ATATGAAGAT
    ATCAAGGAAG TGCATAAACG GAGATATCTC CTGCAGCCTA TCGCTGTGGA AGTTTTCTCT
    GGAGATGGAC GAAACTACCT CCTTGCTTTT CAGAAAGGAA TCAGAAACAA AGTTTATCAA
    AGGTTTTTGG CTGTAGTGCC ATCTCTCACT GACAGTTCAG AGTCTGTGTC TGGGCAGCGG
    CCGAACACCA GCGTGGAGCA GGGATCTGGG TTGCTTAGCA CTCTGGTTGG AGAGAAGTCT
    GTGACTCAGA GATGGGAGAG AGGTGAAATC AGCAACTTTC AGTATTTGAT GCACTTGAAC
    ACTTTGGCTG GAAGATCGTA TAATGACCTC ATGCAATATC CTGTCTTCCC CTGGATCCTT
    GCAGATTACG ACTCGGAGGA GGTCGATCTT ACTAATCCCA AGACATTTAG AAACCTGGCC
    AAGCCAATGG GAGCACAGAC AGATGAACGA TTAGCTCAGT ATAAGAAGCG ATATAAAGAC
    TGGGAAGATC CTAATGGAGA AACTCCAGCC TACCACTATG GGACCCACTA TTCATCAGCC
    ATGATTGTGG CTTCATACCT TGTCAGAATG GAGCCCTTCA CACAGATATT CCTAAGGCTA
    CAGGGAGGCC ACTTTGACCT GGCTGACCGG ATGTTTCACA GCGTGCGTGA GGCCTGGTAC
    TCAGCGTCAA AGCACAACAT GGCAGATGTA AAGGAACTCA TCCCAGAATT TTTTTACTTA
    CCAGAGTTCC TGTTCAATTC CAACAACTTT GACCTTGGCT GTAAACAAAA CGGCACCAAA
    CTTGGAGATG TTATCCTTCC ACCCTGGGCA AAAGGGGACC CACGAGAATT CATCCGGGTG
    CACCGTGAGG CTCTGGAATG TGACTACGTG AGTGCCCATC TGCATGAGTG GATCGACTTA
    ATCTTCGGCT ATAAGCAGCA AGGTCCTGCC GCAGTAGAAG CTGTAAACGT CTTCCATCAC
    CTTTTCTATG AGGGTCAAGT GGATATCTAC AACATAAATG ACCCCCTCAA GGAAACAGCC
    ACCATAGGGT TCATTAATAA CTTTGGGCAG ATCCCTAAAC AGTTATTTAA AAAACCTCAT
    CCACCCAAGC GAGTGAGGAG TCGACTCAAC GGAGACAATG CGGGGGCCTC CGTCCCGCCA
    GGGTCTGCCA GTGACAAGAT CTTTTTTCAT CATCTGGACA ACCTGAGGCC TTCACTAACA
    CCTGTAAAAG AACTCAAAGA GCCTGTGGGA CAAATCGTGT GTACAGACAA AGGCATTCTT
    GCGGTAGAAC AGAATAAGGT TCTTATCCCA CCAACCTGGA ACAAGACTTT CGCCTGGGGC
    TACGCAGACC TCAGCTGCAG GCTGGGGACC TATGAGTCAG ACAAGGCAGT GACTGTTTAT
    GAATGTTTGT CGGAGTGGGG CCAGATTCTC TGTGCGATCT GCCCCAACCC CAAGCTGGTC
    ATCACGGGTG GAACAAGTAC GGTGGTGTGT GTTTGGGAGA TGAGTACCTC GAAAGAAAAG
    GCTAAGACTC TCACTCTCAA ACAGGCCTTA CTTGGCCACA CGGATACGGT TACCTGTGCC
    ACCGCGTCGT TAGCCTATCA CATCATCGTC AGCGGGTCCC GAGACCGAAC CTGCATCATC
    TGGGACCTGA ACAAGCTGTC ATTCCTAACC CAGCTCCGAG GCCACCGGGC TCCCGTGTCT
    GCTCTGTGTA TCAATGAATT AACCGGGGAC ATCGTGTCCT GCGCCGGCAC GTACATCCAT
    GTGTGGAGCA TAAACGGGAA CCCCGTCGTG AGTGTGAACA CGTTCACAGG CAGGAGCCAG
    CAGATCGTCT GTTGCTGTGT GTCGGAGATG AACGAGTGGG ACACGCAGAA CGTCATCGTC
    ACGGGACACT CGGATGGCGT GGTCCGGTTT TGGAGAATGG AATTTTTGCA AGTTCCTGAA
    ACACCAGCCC CTGAGCCTGC TGAAGTCCTC GAAATGCAGG AAGACTGTCC AGAAGCACAA
    ATCGGGCAAG AAGCCCAGGA CGAGGACAGT AGCGATTCCG AGGCCGACGA GCAGAGCGTC
    AGCCAGGACC CCAAGGACAC GCCCAGCCAG CCCAGCAGCA CCAGCCACCG GCCCCGGGCA
    GCCTCCTGCA GAGCCACAGC CACCTGGTAT AACGACAGTG GCTCCGACGA CTCCAGGCGC
    TGGTCCGATC AGCTCAGCCT CGATGAGAAA GACGGCTTCA TATTTGTGAA CTATTCAGAG
    GGCCAGAGCA GACCTCATCT TCAGACTCCC CTTAACCACC CCCACCCCAA TCCCATCGAG
    GCGCGGAATT ACAGCCGATT GAAGCCTGGG TACCGCTGGG AACGGCAGCT CGTGTTCAGG
    AGTAAGCTGA CCATGCACAC GGCCTTTGAC CGGAAGGACA ATGCTCACCC TGCCGAGATC
    ACTGCATTGG GCGTCTCCAA GGACCACAGC AGGATCCTCG TGGGCGACAG TCGGGGCCGG
    GTGTTCAGCT GGTCTGTGAG CGACCAGCCC GGCCGCTCTG CCGCTGACCA CTGGGTGAAG
    GACGAAGGAG GGGACAGCTG TTCAGGCTGC TCCGTGCGGT TCTCTCTCAC GGAAAGACGG
    CACCATTGCA GGAACTGTGG TCAGCTCTTC TGCCAGAAGT GCAGCCGATT TCAGTCAGAA
    ATCAAGCGCT TGAAAATCTC CTCCCCAGTA CGTGTCTGTC AGAACTGTTA CTACAACTTA
    CAGCATGAGC GGAGTTCAGA AGACGGGCCT CGGAATTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90116
    Clone ID Related Accession (Same CDS sequence) XM_015463825.1 , XM_015471705.1
    Accession Version XM_015471705.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WD repeat and FYVE domain-containing protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103983.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGTC GTGCAGAACA GATCCCTCCT TGCCTGACAA GTGAGTCTTC TCTTCCCTCT
    CCTTGGGGGA CTCCAGCTTT GTCCAGGAAA AGGCACGCGT ATCATTCTGT TTCTACTCCC
    CCCGTCTACC CTCCTAAAAA CGTTGCTGAC CTGAAACCAC ACGTGGCAAC TTCATCTCTC
    TCAAGTTCTG ATGCCGTCAT CATCCATCCT GGAGCCATGC TTGCTATGCT GGATCTCCTG
    GCCTCTGTTG GGTCAGTGAC ACAGCCAGAA CATGCCTTGG ACCTTCAACT TGCCGTGGCC
    AATATTTTAC AATCCCTGGT GCACACGGAA AGAAACCAGC AGGTCATGTG TGAAGCTGGT
    CTCCATGCAC GACTGCTGCA GCGGTGCAGT GCCGCTCTGG CTGACGAGGA CCACTCGCTG
    CACCCACCCC TGCAGCGCAT GTTCGAGCGA TTAGCCTCTC AAGCGCTGGA GCCCATGGTG
    CTCAGGGAGT TTTTGCGTTT GGCAAGTCCA TTAAATTGTG GTGCCTGGGA CAAAAAACTG
    CTAAAACAAT ATAGGGTCCA CAAACCAAGT TCACTGAGTT TTGAGCCAGA GATGAGAAGC
    AGTATGATCA CATCCATGGA AGGTCTGGGT TCTGATAATG TTTTCAGCTT ACATGAAGAT
    AACCATTACC GGATAAGCAA GAGCCTAGTG AAATCTGCAG AAGGAAGTAC TGTACCCCTA
    ACCAGAGTGA AATGTCTGGT CTCCATGACA ACCCCACATG ACATCAGACT TCATGGGTCA
    TCAGTTACTC CAGCTTTTGT TGAATTTGAC ACATCCCTTG AAGGGTTTGG CTGCCTGTTC
    CTGCCCAGTC TGGCCCCTCA TAATGCCCCT ACAAACAATG CTGTCACAAC AGGTCTTATC
    GATGGGGCCG TGGTCAGCGG TATTGGTTCT GGTGAACGAT TCTTCCCTCC TCCATCTGGC
    TTAAGCTACT CCAGCTGGTT TTGCATTGAA CATTTTAGTT CTCCTCCCAG TAACCACCCA
    GTCAGACTCC TTACTGTTGT TCGCCGAGCA AATTCTTCTG AGCACCATTA TGTATGCCTT
    GCCATAGTTC TCTCTGCAAA AGATCGGTCT CTGATTGTCT CCACCAAAGA GGAACTACTC
    CAAAATTATG TTGATGATTT TAGTGAAGAA TCCTCTTTCT ATGAGATTCT GCCATGCTGT
    GCTCGCTTCC GTTGTGGAGA ACTGATCGTT GAGGGACAGT GGCATCACCT GGTTCTGGTG
    ATGAGCAAAG GGATGCTGAA GAACAGCACT GCAACCCTCT ACATCGACGG CCAGCTTGTC
    AGCACTGTGA AGCTTCATTA TGTTCACAGT ACTCCCGGGG GCTCAGGCTC TGCAAACCCA
    CCAGTGGTTA GCACAGTCTA TGCCTACATT GGTACTCCGC CTGCCCAGCG CCAAATTGCT
    TCATTGGTTT GGCGCCTGGG ACCTACACAC TTTCTAGAAG AAGTATTACC TCCTTCAAAT
    GTTACTACCA TTTATGAACT AGGACCAAAT TACGTGGGAA GCTTTCAGGC CGTATGTATG
    CCATGTAAAG ATGCAAAATC TGAAGGTGTG GTCCCATCTC CTGTGTCATT AGTACCAGAG
    GAAAAGGTGT CATTCGGCCT CTATGCACTG TCCGTGTCCG CCTTGACAGT GGCGAGAATC
    CGGAAGGTGT ACAACAAGCT GGACAGCAAA GCCATCGCTA AGCAGTTAGG CATTTCCTCC
    CATGAGAACG CCACTCCTGT GAAGCTGATC CACAACTCCG CAGGACACCT CAATGGGCCC
    GCACGGACAA TCGGGGCCAG TCTGATTGGA TACTTGGGAG TAAGAACATT TGTTCCTAAG
    CCTGTTGCCA CTACTTTGCA GTACGTTGGT GGGGCCGCAG CCATCCTGGG CCTGGTAGCC
    ATGGCTTCTG ATGTGGAAGG GCTGTATGCA GCGGTCAAGG CCCTAGTTTG TGTGGTCAAG
    AGTAACCCAC TAGCCAGCAA AGAAATGGAA AGAATCCGGG GCTACCAGTT GCTGGCAATG
    CTGTTTAAGA AGAAACGCTC CCTTCTTAAC AGCCACATTC TCCATCTCAC TTTTTCCTTG
    GTGGGAACTG TTGACAGTGG ACATGAGACT TCCATTATTC CAAATTCAAC AGCTTTCCAG
    GATCTACTTT GTGATTTTGA AGTCTGGCTC CATGCTCCAT ATGAACTTCA TCTCTCCCTA
    TTTGAACACT TTATTGAATT GCTCACAGAG TCCAGTGAAG CTTCAAAGAA TGCCAAGTTG
    ATGAGGGAAT TCCAGCTAAT CCCAAAGCTG CTACTGACTC TTCGAGATAT GTCTCTATCC
    CAGCCTACCA TTGCTGCTAT TAGTAACGTG CTGAGCTTCT TACTGCAGGG TTTTCCCAAC
    AGCAATGATC TACTCAGGTT TGGCCAGTTC ATTTCTTCTA CTTTACCAAC CTTTGCAGTT
    TGTGAGAAGT TTGTAGTTAT GGAAATAAAC CACGAAGAGA AGCCTGACAC TGGAACTGAA
    GAAGAGTTTG GAGGTCTTGT ATCTGCTAAT CTTATACTTT TGAGGAACAG ACTTCTGGAT
    ATCTTACTAA AACTGGTTTA CACATCTAAA GAAAAGACAA GCATTAATTT GCAAGCTTGT
    GAAGAGCTGG TCAGGACGCT GGGCTTTGAC TGGATTATGA TGTTTATGGA AGAACACTTG
    CATTCTACCA CAGTCACAGC AGCCATGAGG ATTCTTGTTG TCCTACTGAG TAATCAGTCT
    ATTCTCATCA AGTTCAAGGA GGGACTCAGT GGTGGAGGCT GGCTGGAGCA GACAGATTCT
    GTCTTAACGA ATAAGATCGG AACTGTATTA GGATTCAATG TGGGCAGGAG TGCTGGCGGA
    CGATCAACTG TCAGGGAGAT TAACCGGGAT GCTTGTCACT TTCCTGGCTT TCCGGTGCTT
    CAGTCATTCC TTCCTAAACA CACTAATGTC CCTGCTCTCT ATTTCCTCCT CATGGCATTG
    TTTCTGCAGC AGCCCGTTAG TGAGCTGCCT GAGAACCTGC AGTTTGATTT AGATTCCATC
    TGGACCTTTA TCTTTGGAGT TCCTGCCTCC AGTGGAACCG TGGTCTCATC TATCCACAAT
    GTGTGCACAG AAGCTGCTTT TTTGTTACTG GGCATGCTCC GAAGCATGCT GAATTCACCT
    TGGCAATCAG AAGAAGAGGG GTCTTGGCTC CGGGAGTATC CTGTGACCCT GATGCAGTTC
    TTCAGATACT TGTATCACAA TGTCCCGGAC CTGGCCTCCA TGTGGATGAA CCCTGATTTC
    CTGTGTGCAC TTGCAGCCAC GGTCTTCCCC TTCAACATCC GCCCTTATTC AGAAATGGTG
    ACTGATCTCG ATGATGAAGT TGGATCTCCA GCTGAAGAAT TTAAAGCCTT TGCAGCAGAC
    ACAGGGATGA ACAGAAGCCA ATCAGAGTAC TGCAACGTGG GCGTCAAGAC ATACCTGACT
    AATCACCCAG CTAAGAAATT TGTTTTTGAC TTCATGCGGG TCTTAATAAT AGACAACCTC
    TGTCTTACAC CTGCCAGCAA GCAGACTCCA CTGATTGATC TTTTGTTGGA GGCTTCTCCT
    GAGAGATCGA CAAGAACGCA GCAAAAAGAA TTTCAGACTT ACATTTTGGA TAGTGTGATG
    GATCATTTGC TTGCAGCTGA TGTTTTACTG GGAGAAGATG CATCTCTTCC TATCACCAGT
    GGAGGAAGCT ACCAGGTATT GGTGAACAAT GTGTTTTATT TCACACAACG TGTGGTGGAC
    AAGCTTTGGC AAGGCATGTT CAACAAAGAA TCTAAACTTC TTATAGATTT TATAATTCAA
    CTAATTGCAC AGTCAAAGAG AAGATCCCAG GGGTTGTCAC TGGATGCAGT CTATCACTGC
    CTCAACAGGA CTATCCTGTA CCAGTTCTCC CGGGCGCACA AAACCGTCCC GCAGCAAGTA
    GCCCTTCTTG ACTCTCTCAG GGTCCTGACT GTCAACAGGA ACTTGATCCT GGGCCCCGGA
    AACCACGACC AAGAATTCAT CAGCTGTCTG GCTCACTGCC TCATTAACCT GCATGTTGGG
    AGCAATGTGG ATGGATTTGG ACTGGAAGCA GAAGCCCGCA TGACTACATG GCATATTATG
    ATCCCCTCGG ACATTGAACC AGATGGTGGT TACAGCCAAG ATATTAGTGA AGGGCGTCAG
    CTTCTCATAA AAGCTGTCAA CAGAGTTTGG ACGGAATTAA TTCATAGTAA GAAACAAGTC
    TTGGAGGAGC TTTTCAAAGT CACTCTACCT GTGAATGAAA GAGGCCACGT GGACATAGCT
    ATAGCAAGGC CGCTCATCGA AGAAGCTAGT TTGAAGTGCT GGCAGAATCA TCTAGCCCAT
    GAAAAGAAGT GCATAAGTCG AGGAGAAGCT TTAATGCCCA CCACACAATC CAAATTGTCC
    CGTGTCAGCA GTGGCTTTGG TCTTTCCAAG TTAACGGGAT CAAGAAGGAA TCGAAAGGAA
    AGCGGGCTTC ATAAACACAG CCTTTCCACC CAGGAGATTT CTCAGTGGAT GTTTACACAC
    ATCGCTGTTG TTCGTGACTT GGTAGACACA CAATATAAGG AATACCAGGA GCGTCAGCAA
    AATGCCCTGA AGTACGTGAC GGAAGAGTGG TGCCAGATCG AGTATGAGCT GCTGCGGGAG
    CGGGGACTCT GGGGCCCCCC CATTGGTTCC CATCTCGACA AGTGGATGTT GGAGATGACA
    GAGGGTCCCT GCAGAATGAG GAAAAAAATG GTGCGAAATG ATATGTTTTA TAACCATTAC
    CCTTACGTGC CAGAAACGGA GCAGGAAACC AACGTGGCGT CTGAGATCCC AAGTAAACAG
    CCTGAGACAC CTGATGATAT CATTCCTCAA AAGAAGCCTG CTCGATACAG AAGAGCCGTA
    AGTTATGACA GTAAAGAATA CTACATGCGA CTAGCCTCCG GCAACCCAGC CATTGTGCAG
    GATGCCATCG TAGAGAGCTC TGAAGGAGAA GCTGCTCAGC AAGAACCAGA ACATGGTGAA
    GACACGATTG CTAAAGTCAA AGGACTGGTC AAGCCACCTC TCAAACGGTC TCGGTCAGCA
    CCGGACGGAG GCGATGAGGA GACACAGGAG CAGTTACAGG ACCAGACAGC TGAGGGCAGC
    TCGATAGAAG AGGAGGAGAA AACAGACAAC GCCACCCTGC TGCGCCTGTT GGAGGAGGGC
    GAAAAGATCC AGCACATGTA CCGCTGTGCT CGAGTCCAGG GCCTTGATAC CAGTGAGGGG
    CTACTCCTCT TTGGTAAAGA GCATTTCTAT GTGATTGATG GATTTACAAT GACAGCAACC
    AGAGAAATAA GAGATATTGA AACCTTACCT CCAAATATGC ATGAGCCGAT TATCCCTAGA
    GGAGCCAGGC AAGGCCCCAG TCAGCTCAAG AGAACGTGCA GTATCTTTGC ATATGAAGAT
    ATCAAGGAAG TGCATAAACG GAGATATCTC CTGCAGCCTA TCGCTGTGGA AGTTTTCTCT
    GGAGATGGAC GAAACTACCT CCTTGCTTTT CAGAAAGGAA TCAGAAACAA AGTTTATCAA
    AGGTTTTTGG CTGTAGTGCC ATCTCTCACT GACAGTTCAG AGTCTGTGTC TGGGCAGCGG
    CCGAACACCA GCGTGGAGCA GGGATCTGGG TTGCTTAGCA CTCTGGTTGG AGAGAAGTCT
    GTGACTCAGA GATGGGAGAG AGGTGAAATC AGCAACTTTC AGTATTTGAT GCACTTGAAC
    ACTTTGGCTG GAAGATCGTA TAATGACCTC ATGCAATATC CTGTCTTCCC CTGGATCCTT
    GCAGATTACG ACTCGGAGGA GGTCGATCTT ACTAATCCCA AGACATTTAG AAACCTGGCC
    AAGCCAATGG GAGCACAGAC AGATGAACGA TTAGCTCAGT ATAAGAAGCG ATATAAAGAC
    TGGGAAGATC CTAATGGAGA AACTCCAGCC TACCACTATG GGACCCACTA TTCATCAGCC
    ATGATTGTGG CTTCATACCT TGTCAGAATG GAGCCCTTCA CACAGATATT CCTAAGGCTA
    CAGGGAGGCC ACTTTGACCT GGCTGACCGG ATGTTTCACA GCGTGCGTGA GGCCTGGTAC
    TCAGCGTCAA AGCACAACAT GGCAGATGTA AAGGAACTCA TCCCAGAATT TTTTTACTTA
    CCAGAGTTCC TGTTCAATTC CAACAACTTT GACCTTGGCT GTAAACAAAA CGGCACCAAA
    CTTGGAGATG TTATCCTTCC ACCCTGGGCA AAAGGGGACC CACGAGAATT CATCCGGGTG
    CACCGTGAGG CTCTGGAATG TGACTACGTG AGTGCCCATC TGCATGAGTG GATCGACTTA
    ATCTTCGGCT ATAAGCAGCA AGGTCCTGCC GCAGTAGAAG CTGTAAACGT CTTCCATCAC
    CTTTTCTATG AGGGTCAAGT GGATATCTAC AACATAAATG ACCCCCTCAA GGAAACAGCC
    ACCATAGGGT TCATTAATAA CTTTGGGCAG ATCCCTAAAC AGTTATTTAA AAAACCTCAT
    CCACCCAAGC GAGTGAGGAG TCGACTCAAC GGAGACAATG CGGGGGCCTC CGTCCCGCCA
    GGGTCTGCCA GTGACAAGAT CTTTTTTCAT CATCTGGACA ACCTGAGGCC TTCACTAACA
    CCTGTAAAAG AACTCAAAGA GCCTGTGGGA CAAATCGTGT GTACAGACAA AGGCATTCTT
    GCGGTAGAAC AGAATAAGGT TCTTATCCCA CCAACCTGGA ACAAGACTTT CGCCTGGGGC
    TACGCAGACC TCAGCTGCAG GCTGGGGACC TATGAGTCAG ACAAGGCAGT GACTGTTTAT
    GAATGTTTGT CGGAGTGGGG CCAGATTCTC TGTGCGATCT GCCCCAACCC CAAGCTGGTC
    ATCACGGGTG GAACAAGTAC GGTGGTGTGT GTTTGGGAGA TGAGTACCTC GAAAGAAAAG
    GCTAAGACTC TCACTCTCAA ACAGGCCTTA CTTGGCCACA CGGATACGGT TACCTGTGCC
    ACCGCGTCGT TAGCCTATCA CATCATCGTC AGCGGGTCCC GAGACCGAAC CTGCATCATC
    TGGGACCTGA ACAAGCTGTC ATTCCTAACC CAGCTCCGAG GCCACCGGGC TCCCGTGTCT
    GCTCTGTGTA TCAATGAATT AACCGGGGAC ATCGTGTCCT GCGCCGGCAC GTACATCCAT
    GTGTGGAGCA TAAACGGGAA CCCCGTCGTG AGTGTGAACA CGTTCACAGG CAGGAGCCAG
    CAGATCGTCT GTTGCTGTGT GTCGGAGATG AACGAGTGGG ACACGCAGAA CGTCATCGTC
    ACGGGACACT CGGATGGCGT GGTCCGGTTT TGGAGAATGG AATTTTTGCA AGTTCCTGAA
    ACACCAGCCC CTGAGCCTGC TGAAGTCCTC GAAATGCAGG AAGACTGTCC AGAAGCACAA
    ATCGGGCAAG AAGCCCAGGA CGAGGACAGT AGCGATTCCG AGGCCGACGA GCAGAGCGTC
    AGCCAGGACC CCAAGGACAC GCCCAGCCAG CCCAGCAGCA CCAGCCACCG GCCCCGGGCA
    GCCTCCTGCA GAGCCACAGC CACCTGGTAT AACGACAGTG GCTCCGACGA CTCCAGGCGC
    TGGTCCGATC AGCTCAGCCT CGATGAGAAA GACGGCTTCA TATTTGTGAA CTATTCAGAG
    GGCCAGAGCA GACCTCATCT TCAGACTCCC CTTAACCACC CCCACCCCAA TCCCATCGAG
    GCGCGGAATT ACAGCCGATT GAAGCCTGGG TACCGCTGGG AACGGCAGCT CGTGTTCAGG
    AGTAAGCTGA CCATGCACAC GGCCTTTGAC CGGAAGGACA ATGCTCACCC TGCCGAGATC
    ACTGCATTGG GCGTCTCCAA GGACCACAGC AGGATCCTCG TGGGCGACAG TCGGGGCCGG
    GTGTTCAGCT GGTCTGTGAG CGACCAGCCC GGCCGCTCTG CCGCTGACCA CTGGGTGAAG
    GACGAAGGAG GGGACAGCTG TTCAGGCTGC TCCGTGCGGT TCTCTCTCAC GGAAAGACGG
    CACCATTGCA GGAACTGTGG TCAGCTCTTC TGCCAGAAGT GCAGCCGATT TCAGTCAGAA
    ATCAAGCGCT TGAAAATCTC CTCCCCAGTA CGTGTCTGTC AGAACTGTTA CTACAACTTA
    CAGCATGAGC GGAGTTCAGA AGACGGGCCT CGGAATTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327191.1
    CDS998..10819
    Translation

    Target ORF information:

    RefSeq Version XM_015471705.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471705.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGTC GTGCAGAACA GATCCCTCCT TGCCTGACAA GTGAGTCTTC TCTTCCCTCT
    CCTTGGGGGA CTCCAGCTTT GTCCAGGAAA AGGCACGCGT ATCATTCTGT TTCTACTCCC
    CCCGTCTACC CTCCTAAAAA CGTTGCTGAC CTGAAACCAC ACGTGGCAAC TTCATCTCTC
    TCAAGTTCTG ATGCCGTCAT CATCCATCCT GGAGCCATGC TTGCTATGCT GGATCTCCTG
    GCCTCTGTTG GGTCAGTGAC ACAGCCAGAA CATGCCTTGG ACCTTCAACT TGCCGTGGCC
    AATATTTTAC AATCCCTGGT GCACACGGAA AGAAACCAGC AGGTCATGTG TGAAGCTGGT
    CTCCATGCAC GACTGCTGCA GCGGTGCAGT GCCGCTCTGG CTGACGAGGA CCACTCGCTG
    CACCCACCCC TGCAGCGCAT GTTCGAGCGA TTAGCCTCTC AAGCGCTGGA GCCCATGGTG
    CTCAGGGAGT TTTTGCGTTT GGCAAGTCCA TTAAATTGTG GTGCCTGGGA CAAAAAACTG
    CTAAAACAAT ATAGGGTCCA CAAACCAAGT TCACTGAGTT TTGAGCCAGA GATGAGAAGC
    AGTATGATCA CATCCATGGA AGGTCTGGGT TCTGATAATG TTTTCAGCTT ACATGAAGAT
    AACCATTACC GGATAAGCAA GAGCCTAGTG AAATCTGCAG AAGGAAGTAC TGTACCCCTA
    ACCAGAGTGA AATGTCTGGT CTCCATGACA ACCCCACATG ACATCAGACT TCATGGGTCA
    TCAGTTACTC CAGCTTTTGT TGAATTTGAC ACATCCCTTG AAGGGTTTGG CTGCCTGTTC
    CTGCCCAGTC TGGCCCCTCA TAATGCCCCT ACAAACAATG CTGTCACAAC AGGTCTTATC
    GATGGGGCCG TGGTCAGCGG TATTGGTTCT GGTGAACGAT TCTTCCCTCC TCCATCTGGC
    TTAAGCTACT CCAGCTGGTT TTGCATTGAA CATTTTAGTT CTCCTCCCAG TAACCACCCA
    GTCAGACTCC TTACTGTTGT TCGCCGAGCA AATTCTTCTG AGCACCATTA TGTATGCCTT
    GCCATAGTTC TCTCTGCAAA AGATCGGTCT CTGATTGTCT CCACCAAAGA GGAACTACTC
    CAAAATTATG TTGATGATTT TAGTGAAGAA TCCTCTTTCT ATGAGATTCT GCCATGCTGT
    GCTCGCTTCC GTTGTGGAGA ACTGATCGTT GAGGGACAGT GGCATCACCT GGTTCTGGTG
    ATGAGCAAAG GGATGCTGAA GAACAGCACT GCAACCCTCT ACATCGACGG CCAGCTTGTC
    AGCACTGTGA AGCTTCATTA TGTTCACAGT ACTCCCGGGG GCTCAGGCTC TGCAAACCCA
    CCAGTGGTTA GCACAGTCTA TGCCTACATT GGTACTCCGC CTGCCCAGCG CCAAATTGCT
    TCATTGGTTT GGCGCCTGGG ACCTACACAC TTTCTAGAAG AAGTATTACC TCCTTCAAAT
    GTTACTACCA TTTATGAACT AGGACCAAAT TACGTGGGAA GCTTTCAGGC CGTATGTATG
    CCATGTAAAG ATGCAAAATC TGAAGGTGTG GTCCCATCTC CTGTGTCATT AGTACCAGAG
    GAAAAGGTGT CATTCGGCCT CTATGCACTG TCCGTGTCCG CCTTGACAGT GGCGAGAATC
    CGGAAGGTGT ACAACAAGCT GGACAGCAAA GCCATCGCTA AGCAGTTAGG CATTTCCTCC
    CATGAGAACG CCACTCCTGT GAAGCTGATC CACAACTCCG CAGGACACCT CAATGGGCCC
    GCACGGACAA TCGGGGCCAG TCTGATTGGA TACTTGGGAG TAAGAACATT TGTTCCTAAG
    CCTGTTGCCA CTACTTTGCA GTACGTTGGT GGGGCCGCAG CCATCCTGGG CCTGGTAGCC
    ATGGCTTCTG ATGTGGAAGG GCTGTATGCA GCGGTCAAGG CCCTAGTTTG TGTGGTCAAG
    AGTAACCCAC TAGCCAGCAA AGAAATGGAA AGAATCCGGG GCTACCAGTT GCTGGCAATG
    CTGTTTAAGA AGAAACGCTC CCTTCTTAAC AGCCACATTC TCCATCTCAC TTTTTCCTTG
    GTGGGAACTG TTGACAGTGG ACATGAGACT TCCATTATTC CAAATTCAAC AGCTTTCCAG
    GATCTACTTT GTGATTTTGA AGTCTGGCTC CATGCTCCAT ATGAACTTCA TCTCTCCCTA
    TTTGAACACT TTATTGAATT GCTCACAGAG TCCAGTGAAG CTTCAAAGAA TGCCAAGTTG
    ATGAGGGAAT TCCAGCTAAT CCCAAAGCTG CTACTGACTC TTCGAGATAT GTCTCTATCC
    CAGCCTACCA TTGCTGCTAT TAGTAACGTG CTGAGCTTCT TACTGCAGGG TTTTCCCAAC
    AGCAATGATC TACTCAGGTT TGGCCAGTTC ATTTCTTCTA CTTTACCAAC CTTTGCAGTT
    TGTGAGAAGT TTGTAGTTAT GGAAATAAAC CACGAAGAGA AGCCTGACAC TGGAACTGAA
    GAAGAGTTTG GAGGTCTTGT ATCTGCTAAT CTTATACTTT TGAGGAACAG ACTTCTGGAT
    ATCTTACTAA AACTGGTTTA CACATCTAAA GAAAAGACAA GCATTAATTT GCAAGCTTGT
    GAAGAGCTGG TCAGGACGCT GGGCTTTGAC TGGATTATGA TGTTTATGGA AGAACACTTG
    CATTCTACCA CAGTCACAGC AGCCATGAGG ATTCTTGTTG TCCTACTGAG TAATCAGTCT
    ATTCTCATCA AGTTCAAGGA GGGACTCAGT GGTGGAGGCT GGCTGGAGCA GACAGATTCT
    GTCTTAACGA ATAAGATCGG AACTGTATTA GGATTCAATG TGGGCAGGAG TGCTGGCGGA
    CGATCAACTG TCAGGGAGAT TAACCGGGAT GCTTGTCACT TTCCTGGCTT TCCGGTGCTT
    CAGTCATTCC TTCCTAAACA CACTAATGTC CCTGCTCTCT ATTTCCTCCT CATGGCATTG
    TTTCTGCAGC AGCCCGTTAG TGAGCTGCCT GAGAACCTGC AGTTTGATTT AGATTCCATC
    TGGACCTTTA TCTTTGGAGT TCCTGCCTCC AGTGGAACCG TGGTCTCATC TATCCACAAT
    GTGTGCACAG AAGCTGCTTT TTTGTTACTG GGCATGCTCC GAAGCATGCT GAATTCACCT
    TGGCAATCAG AAGAAGAGGG GTCTTGGCTC CGGGAGTATC CTGTGACCCT GATGCAGTTC
    TTCAGATACT TGTATCACAA TGTCCCGGAC CTGGCCTCCA TGTGGATGAA CCCTGATTTC
    CTGTGTGCAC TTGCAGCCAC GGTCTTCCCC TTCAACATCC GCCCTTATTC AGAAATGGTG
    ACTGATCTCG ATGATGAAGT TGGATCTCCA GCTGAAGAAT TTAAAGCCTT TGCAGCAGAC
    ACAGGGATGA ACAGAAGCCA ATCAGAGTAC TGCAACGTGG GCGTCAAGAC ATACCTGACT
    AATCACCCAG CTAAGAAATT TGTTTTTGAC TTCATGCGGG TCTTAATAAT AGACAACCTC
    TGTCTTACAC CTGCCAGCAA GCAGACTCCA CTGATTGATC TTTTGTTGGA GGCTTCTCCT
    GAGAGATCGA CAAGAACGCA GCAAAAAGAA TTTCAGACTT ACATTTTGGA TAGTGTGATG
    GATCATTTGC TTGCAGCTGA TGTTTTACTG GGAGAAGATG CATCTCTTCC TATCACCAGT
    GGAGGAAGCT ACCAGGTATT GGTGAACAAT GTGTTTTATT TCACACAACG TGTGGTGGAC
    AAGCTTTGGC AAGGCATGTT CAACAAAGAA TCTAAACTTC TTATAGATTT TATAATTCAA
    CTAATTGCAC AGTCAAAGAG AAGATCCCAG GGGTTGTCAC TGGATGCAGT CTATCACTGC
    CTCAACAGGA CTATCCTGTA CCAGTTCTCC CGGGCGCACA AAACCGTCCC GCAGCAAGTA
    GCCCTTCTTG ACTCTCTCAG GGTCCTGACT GTCAACAGGA ACTTGATCCT GGGCCCCGGA
    AACCACGACC AAGAATTCAT CAGCTGTCTG GCTCACTGCC TCATTAACCT GCATGTTGGG
    AGCAATGTGG ATGGATTTGG ACTGGAAGCA GAAGCCCGCA TGACTACATG GCATATTATG
    ATCCCCTCGG ACATTGAACC AGATGGTGGT TACAGCCAAG ATATTAGTGA AGGGCGTCAG
    CTTCTCATAA AAGCTGTCAA CAGAGTTTGG ACGGAATTAA TTCATAGTAA GAAACAAGTC
    TTGGAGGAGC TTTTCAAAGT CACTCTACCT GTGAATGAAA GAGGCCACGT GGACATAGCT
    ATAGCAAGGC CGCTCATCGA AGAAGCTAGT TTGAAGTGCT GGCAGAATCA TCTAGCCCAT
    GAAAAGAAGT GCATAAGTCG AGGAGAAGCT TTAATGCCCA CCACACAATC CAAATTGTCC
    CGTGTCAGCA GTGGCTTTGG TCTTTCCAAG TTAACGGGAT CAAGAAGGAA TCGAAAGGAA
    AGCGGGCTTC ATAAACACAG CCTTTCCACC CAGGAGATTT CTCAGTGGAT GTTTACACAC
    ATCGCTGTTG TTCGTGACTT GGTAGACACA CAATATAAGG AATACCAGGA GCGTCAGCAA
    AATGCCCTGA AGTACGTGAC GGAAGAGTGG TGCCAGATCG AGTATGAGCT GCTGCGGGAG
    CGGGGACTCT GGGGCCCCCC CATTGGTTCC CATCTCGACA AGTGGATGTT GGAGATGACA
    GAGGGTCCCT GCAGAATGAG GAAAAAAATG GTGCGAAATG ATATGTTTTA TAACCATTAC
    CCTTACGTGC CAGAAACGGA GCAGGAAACC AACGTGGCGT CTGAGATCCC AAGTAAACAG
    CCTGAGACAC CTGATGATAT CATTCCTCAA AAGAAGCCTG CTCGATACAG AAGAGCCGTA
    AGTTATGACA GTAAAGAATA CTACATGCGA CTAGCCTCCG GCAACCCAGC CATTGTGCAG
    GATGCCATCG TAGAGAGCTC TGAAGGAGAA GCTGCTCAGC AAGAACCAGA ACATGGTGAA
    GACACGATTG CTAAAGTCAA AGGACTGGTC AAGCCACCTC TCAAACGGTC TCGGTCAGCA
    CCGGACGGAG GCGATGAGGA GACACAGGAG CAGTTACAGG ACCAGACAGC TGAGGGCAGC
    TCGATAGAAG AGGAGGAGAA AACAGACAAC GCCACCCTGC TGCGCCTGTT GGAGGAGGGC
    GAAAAGATCC AGCACATGTA CCGCTGTGCT CGAGTCCAGG GCCTTGATAC CAGTGAGGGG
    CTACTCCTCT TTGGTAAAGA GCATTTCTAT GTGATTGATG GATTTACAAT GACAGCAACC
    AGAGAAATAA GAGATATTGA AACCTTACCT CCAAATATGC ATGAGCCGAT TATCCCTAGA
    GGAGCCAGGC AAGGCCCCAG TCAGCTCAAG AGAACGTGCA GTATCTTTGC ATATGAAGAT
    ATCAAGGAAG TGCATAAACG GAGATATCTC CTGCAGCCTA TCGCTGTGGA AGTTTTCTCT
    GGAGATGGAC GAAACTACCT CCTTGCTTTT CAGAAAGGAA TCAGAAACAA AGTTTATCAA
    AGGTTTTTGG CTGTAGTGCC ATCTCTCACT GACAGTTCAG AGTCTGTGTC TGGGCAGCGG
    CCGAACACCA GCGTGGAGCA GGGATCTGGG TTGCTTAGCA CTCTGGTTGG AGAGAAGTCT
    GTGACTCAGA GATGGGAGAG AGGTGAAATC AGCAACTTTC AGTATTTGAT GCACTTGAAC
    ACTTTGGCTG GAAGATCGTA TAATGACCTC ATGCAATATC CTGTCTTCCC CTGGATCCTT
    GCAGATTACG ACTCGGAGGA GGTCGATCTT ACTAATCCCA AGACATTTAG AAACCTGGCC
    AAGCCAATGG GAGCACAGAC AGATGAACGA TTAGCTCAGT ATAAGAAGCG ATATAAAGAC
    TGGGAAGATC CTAATGGAGA AACTCCAGCC TACCACTATG GGACCCACTA TTCATCAGCC
    ATGATTGTGG CTTCATACCT TGTCAGAATG GAGCCCTTCA CACAGATATT CCTAAGGCTA
    CAGGGAGGCC ACTTTGACCT GGCTGACCGG ATGTTTCACA GCGTGCGTGA GGCCTGGTAC
    TCAGCGTCAA AGCACAACAT GGCAGATGTA AAGGAACTCA TCCCAGAATT TTTTTACTTA
    CCAGAGTTCC TGTTCAATTC CAACAACTTT GACCTTGGCT GTAAACAAAA CGGCACCAAA
    CTTGGAGATG TTATCCTTCC ACCCTGGGCA AAAGGGGACC CACGAGAATT CATCCGGGTG
    CACCGTGAGG CTCTGGAATG TGACTACGTG AGTGCCCATC TGCATGAGTG GATCGACTTA
    ATCTTCGGCT ATAAGCAGCA AGGTCCTGCC GCAGTAGAAG CTGTAAACGT CTTCCATCAC
    CTTTTCTATG AGGGTCAAGT GGATATCTAC AACATAAATG ACCCCCTCAA GGAAACAGCC
    ACCATAGGGT TCATTAATAA CTTTGGGCAG ATCCCTAAAC AGTTATTTAA AAAACCTCAT
    CCACCCAAGC GAGTGAGGAG TCGACTCAAC GGAGACAATG CGGGGGCCTC CGTCCCGCCA
    GGGTCTGCCA GTGACAAGAT CTTTTTTCAT CATCTGGACA ACCTGAGGCC TTCACTAACA
    CCTGTAAAAG AACTCAAAGA GCCTGTGGGA CAAATCGTGT GTACAGACAA AGGCATTCTT
    GCGGTAGAAC AGAATAAGGT TCTTATCCCA CCAACCTGGA ACAAGACTTT CGCCTGGGGC
    TACGCAGACC TCAGCTGCAG GCTGGGGACC TATGAGTCAG ACAAGGCAGT GACTGTTTAT
    GAATGTTTGT CGGAGTGGGG CCAGATTCTC TGTGCGATCT GCCCCAACCC CAAGCTGGTC
    ATCACGGGTG GAACAAGTAC GGTGGTGTGT GTTTGGGAGA TGAGTACCTC GAAAGAAAAG
    GCTAAGACTC TCACTCTCAA ACAGGCCTTA CTTGGCCACA CGGATACGGT TACCTGTGCC
    ACCGCGTCGT TAGCCTATCA CATCATCGTC AGCGGGTCCC GAGACCGAAC CTGCATCATC
    TGGGACCTGA ACAAGCTGTC ATTCCTAACC CAGCTCCGAG GCCACCGGGC TCCCGTGTCT
    GCTCTGTGTA TCAATGAATT AACCGGGGAC ATCGTGTCCT GCGCCGGCAC GTACATCCAT
    GTGTGGAGCA TAAACGGGAA CCCCGTCGTG AGTGTGAACA CGTTCACAGG CAGGAGCCAG
    CAGATCGTCT GTTGCTGTGT GTCGGAGATG AACGAGTGGG ACACGCAGAA CGTCATCGTC
    ACGGGACACT CGGATGGCGT GGTCCGGTTT TGGAGAATGG AATTTTTGCA AGTTCCTGAA
    ACACCAGCCC CTGAGCCTGC TGAAGTCCTC GAAATGCAGG AAGACTGTCC AGAAGCACAA
    ATCGGGCAAG AAGCCCAGGA CGAGGACAGT AGCGATTCCG AGGCCGACGA GCAGAGCGTC
    AGCCAGGACC CCAAGGACAC GCCCAGCCAG CCCAGCAGCA CCAGCCACCG GCCCCGGGCA
    GCCTCCTGCA GAGCCACAGC CACCTGGTAT AACGACAGTG GCTCCGACGA CTCCAGGCGC
    TGGTCCGATC AGCTCAGCCT CGATGAGAAA GACGGCTTCA TATTTGTGAA CTATTCAGAG
    GGCCAGAGCA GACCTCATCT TCAGACTCCC CTTAACCACC CCCACCCCAA TCCCATCGAG
    GCGCGGAATT ACAGCCGATT GAAGCCTGGG TACCGCTGGG AACGGCAGCT CGTGTTCAGG
    AGTAAGCTGA CCATGCACAC GGCCTTTGAC CGGAAGGACA ATGCTCACCC TGCCGAGATC
    ACTGCATTGG GCGTCTCCAA GGACCACAGC AGGATCCTCG TGGGCGACAG TCGGGGCCGG
    GTGTTCAGCT GGTCTGTGAG CGACCAGCCC GGCCGCTCTG CCGCTGACCA CTGGGTGAAG
    GACGAAGGAG GGGACAGCTG TTCAGGCTGC TCCGTGCGGT TCTCTCTCAC GGAAAGACGG
    CACCATTGCA GGAACTGTGG TCAGCTCTTC TGCCAGAAGT GCAGCCGATT TCAGTCAGAA
    ATCAAGCGCT TGAAAATCTC CTCCCCAGTA CGTGTCTGTC AGAACTGTTA CTACAACTTA
    CAGCATGAGC GGAGTTCAGA AGACGGGCCT CGGAATTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90117
    Clone ID Related Accession (Same CDS sequence) XM_015471706.1 , XM_015463826.1
    Accession Version XM_015471706.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WD repeat and FYVE domain-containing protein 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103983.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGGC ACGCGTATCA TTCTGTTTCT ACTCCCCCCG TCTACCCTCC TAAAAACGTT
    GCTGACCTGA AACCACACGT GGCAACTTCA TCTCTCTCAA GTTCTGATGC CGTCATCATC
    CATCCTGGAG CCATGCTTGC TATGCTGGAT CTCCTGGCCT CTGTTGGGTC AGTGACACAG
    CCAGAACATG CCTTGGACCT TCAACTTGCC GTGGCCAATA TTTTACAATC CCTGGTGCAC
    ACGGAAAGAA ACCAGCAGGT CATGTGTGAA GCTGGTCTCC ATGCACGACT GCTGCAGCGG
    TGCAGTGCCG CTCTGGCTGA CGAGGACCAC TCGCTGCACC CACCCCTGCA GCGCATGTTC
    GAGCGATTAG CCTCTCAAGC GCTGGAGCCC ATGGTGCTCA GGGAGTTTTT GCGTTTGGCA
    AGTCCATTAA ATTGTGGTGC CTGGGACAAA AAACTGCTAA AACAATATAG GGTCCACAAA
    CCAAGTTCAC TGAGTTTTGA GCCAGAGATG AGAAGCAGTA TGATCACATC CATGGAAGGT
    CTGGGTTCTG ATAATGTTTT CAGCTTACAT GAAGATAACC ATTACCGGAT AAGCAAGAGC
    CTAGTGAAAT CTGCAGAAGG AAGTACTGTA CCCCTAACCA GAGTGAAATG TCTGGTCTCC
    ATGACAACCC CACATGACAT CAGACTTCAT GGGTCATCAG TTACTCCAGC TTTTGTTGAA
    TTTGACACAT CCCTTGAAGG GTTTGGCTGC CTGTTCCTGC CCAGTCTGGC CCCTCATAAT
    GCCCCTACAA ACAATGCTGT CACAACAGGT CTTATCGATG GGGCCGTGGT CAGCGGTATT
    GGTTCTGGTG AACGATTCTT CCCTCCTCCA TCTGGCTTAA GCTACTCCAG CTGGTTTTGC
    ATTGAACATT TTAGTTCTCC TCCCAGTAAC CACCCAGTCA GACTCCTTAC TGTTGTTCGC
    CGAGCAAATT CTTCTGAGCA CCATTATGTA TGCCTTGCCA TAGTTCTCTC TGCAAAAGAT
    CGGTCTCTGA TTGTCTCCAC CAAAGAGGAA CTACTCCAAA ATTATGTTGA TGATTTTAGT
    GAAGAATCCT CTTTCTATGA GATTCTGCCA TGCTGTGCTC GCTTCCGTTG TGGAGAACTG
    ATCGTTGAGG GACAGTGGCA TCACCTGGTT CTGGTGATGA GCAAAGGGAT GCTGAAGAAC
    AGCACTGCAA CCCTCTACAT CGACGGCCAG CTTGTCAGCA CTGTGAAGCT TCATTATGTT
    CACAGTACTC CCGGGGGCTC AGGCTCTGCA AACCCACCAG TGGTTAGCAC AGTCTATGCC
    TACATTGGTA CTCCGCCTGC CCAGCGCCAA ATTGCTTCAT TGGTTTGGCG CCTGGGACCT
    ACACACTTTC TAGAAGAAGT ATTACCTCCT TCAAATGTTA CTACCATTTA TGAACTAGGA
    CCAAATTACG TGGGAAGCTT TCAGGCCGTA TGTATGCCAT GTAAAGATGC AAAATCTGAA
    GGTGTGGTCC CATCTCCTGT GTCATTAGTA CCAGAGGAAA AGGTGTCATT CGGCCTCTAT
    GCACTGTCCG TGTCCGCCTT GACAGTGGCG AGAATCCGGA AGGTGTACAA CAAGCTGGAC
    AGCAAAGCCA TCGCTAAGCA GTTAGGCATT TCCTCCCATG AGAACGCCAC TCCTGTGAAG
    CTGATCCACA ACTCCGCAGG ACACCTCAAT GGGCCCGCAC GGACAATCGG GGCCAGTCTG
    ATTGGATACT TGGGAGTAAG AACATTTGTT CCTAAGCCTG TTGCCACTAC TTTGCAGTAC
    GTTGGTGGGG CCGCAGCCAT CCTGGGCCTG GTAGCCATGG CTTCTGATGT GGAAGGGCTG
    TATGCAGCGG TCAAGGCCCT AGTTTGTGTG GTCAAGAGTA ACCCACTAGC CAGCAAAGAA
    ATGGAAAGAA TCCGGGGCTA CCAGTTGCTG GCAATGCTGT TTAAGAAGAA ACGCTCCCTT
    CTTAACAGCC ACATTCTCCA TCTCACTTTT TCCTTGGTGG GAACTGTTGA CAGTGGACAT
    GAGACTTCCA TTATTCCAAA TTCAACAGCT TTCCAGGATC TACTTTGTGA TTTTGAAGTC
    TGGCTCCATG CTCCATATGA ACTTCATCTC TCCCTATTTG AACACTTTAT TGAATTGCTC
    ACAGAGTCCA GTGAAGCTTC AAAGAATGCC AAGTTGATGA GGGAATTCCA GCTAATCCCA
    AAGCTGCTAC TGACTCTTCG AGATATGTCT CTATCCCAGC CTACCATTGC TGCTATTAGT
    AACGTGCTGA GCTTCTTACT GCAGGGTTTT CCCAACAGCA ATGATCTACT CAGGTTTGGC
    CAGTTCATTT CTTCTACTTT ACCAACCTTT GCAGTTTGTG AGAAGTTTGT AGTTATGGAA
    ATAAACCACG AAGAGAAGCC TGACACTGGA ACTGAAGAAG AGTTTGGAGG TCTTGTATCT
    GCTAATCTTA TACTTTTGAG GAACAGACTT CTGGATATCT TACTAAAACT GGTTTACACA
    TCTAAAGAAA AGACAAGCAT TAATTTGCAA GCTTGTGAAG AGCTGGTCAG GACGCTGGGC
    TTTGACTGGA TTATGATGTT TATGGAAGAA CACTTGCATT CTACCACAGT CACAGCAGCC
    ATGAGGATTC TTGTTGTCCT ACTGAGTAAT CAGTCTATTC TCATCAAGTT CAAGGAGGGA
    CTCAGTGGTG GAGGCTGGCT GGAGCAGACA GATTCTGTCT TAACGAATAA GATCGGAACT
    GTATTAGGAT TCAATGTGGG CAGGAGTGCT GGCGGACGAT CAACTGTCAG GGAGATTAAC
    CGGGATGCTT GTCACTTTCC TGGCTTTCCG GTGCTTCAGT CATTCCTTCC TAAACACACT
    AATGTCCCTG CTCTCTATTT CCTCCTCATG GCATTGTTTC TGCAGCAGCC CGTTAGTGAG
    CTGCCTGAGA ACCTGCAGGT CAGTGTGCCT GTCGTCAGCA GCCGATGTAA GCAGGGTTGC
    CAGTTTGATT TAGATTCCAT CTGGACCTTT ATCTTTGGAG TTCCTGCCTC CAGTGGAACC
    GTGGTCTCAT CTATCCACAA TGTGTGCACA GAAGCTGCTT TTTTGTTACT GGGCATGCTC
    CGAAGCATGC TGAATTCACC TTGGCAATCA GAAGAAGAGG GGTCTTGGCT CCGGGAGTAT
    CCTGTGACCC TGATGCAGTT CTTCAGATAC TTGTATCACA ATGTCCCGGA CCTGGCCTCC
    ATGTGGATGA ACCCTGATTT CCTGTGTGCA CTTGCAGCCA CGGTCTTCCC CTTCAACATC
    CGCCCTTATT CAGAAATGGT GACTGATCTC GATGATGAAG TTGGATCTCC AGCTGAAGAA
    TTTAAAGCCT TTGCAGCAGA CACAGGGATG AACAGAAGCC AATCAGAGTA CTGCAACGTG
    GGCGTCAAGA CATACCTGAC TAATCACCCA GCTAAGAAAT TTGTTTTTGA CTTCATGCGG
    GTCTTAATAA TAGACAACCT CTGTCTTACA CCTGCCAGCA AGCAGACTCC ACTGATTGAT
    CTTTTGTTGG AGGCTTCTCC TGAGAGATCG ACAAGAACGC AGCAAAAAGA ATTTCAGACT
    TACATTTTGG ATAGTGTGAT GGATCATTTG CTTGCAGCTG ATGTTTTACT GGGAGAAGAT
    GCATCTCTTC CTATCACCAG TGGAGGAAGC TACCAGGTAT TGGTGAACAA TGTGTTTTAT
    TTCACACAAC GTGTGGTGGA CAAGCTTTGG CAAGGCATGT TCAACAAAGA ATCTAAACTT
    CTTATAGATT TTATAATTCA ACTAATTGCA CAGTCAAAGA GAAGATCCCA GGGGTTGTCA
    CTGGATGCAG TCTATCACTG CCTCAACAGG ACTATCCTGT ACCAGTTCTC CCGGGCGCAC
    AAAACCGTCC CGCAGCAAGT AGCCCTTCTT GACTCTCTCA GGGTCCTGAC TGTCAACAGG
    AACTTGATCC TGGGCCCCGG AAACCACGAC CAAGAATTCA TCAGCTGTCT GGCTCACTGC
    CTCATTAACC TGCATGTTGG GAGCAATGTG GATGGATTTG GACTGGAAGC AGAAGCCCGC
    ATGACTACAT GGCATATTAT GATCCCCTCG GACATTGAAC CAGATGGTGG TTACAGCCAA
    GATATTAGTG AAGGGCGTCA GCTTCTCATA AAAGCTGTCA ACAGAGTTTG GACGGAATTA
    ATTCATAGTA AGAAACAAGT CTTGGAGGAG CTTTTCAAAG TCACTCTACC TGTGAATGAA
    AGAGGCCACG TGGACATAGC TATAGCAAGG CCGCTCATCG AAGAAGCTAG TTTGAAGTGC
    TGGCAGAATC ATCTAGCCCA TGAAAAGAAG TGCATAAGTC GAGGAGAAGC TTTAATGCCC
    ACCACACAAT CCAAATTGTC CCGTGTCAGC AGTGGCTTTG GTCTTTCCAA GTTAACGGGA
    TCAAGAAGGA ATCGAAAGGA AAGCGGGCTT CATAAACACA GCCTTTCCAC CCAGGAGATT
    TCTCAGTGGA TGTTTACACA CATCGCTGTT GTTCGTGACT TGGTAGACAC ACAATATAAG
    GAATACCAGG AGCGTCAGCA AAATGCCCTG AAGTACGTGA CGGAAGAGTG GTGCCAGATC
    GAGTATGAGC TGCTGCGGGA GCGGGGACTC TGGGGCCCCC CCATTGGTTC CCATCTCGAC
    AAGTGGATGT TGGAGATGAC AGAGGGTCCC TGCAGAATGA GGAAAAAAAT GGTGCGAAAT
    GATATGTTTT ATAACCATTA CCCTTACGTG CCAGAAACGG AGCAGGAAAC CAACGTGGCG
    TCTGAGATCC CAAGTAAACA GCCTGAGACA CCTGATGATA TCATTCCTCA AAAGAAGCCT
    GCTCGATACA GAAGAGCCGT AAGTTATGAC AGTAAAGAAT ACTACATGCG ACTAGCCTCC
    GGCAACCCAG CCATTGTGCA GGATGCCATC GTAGAGAGCT CTGAAGGAGA AGCTGCTCAG
    CAAGAACCAG AACATGGTGA AGACACGATT GCTAAAGTCA AAGGACTGGT CAAGCCACCT
    CTCAAACGGT CTCGGTCAGC ACCGGACGGA GGCGATGAGG AGACACAGGA GCAGTTACAG
    GACCAGACAG CTGAGGGCAG CTCGATAGAA GAGGAGGAGA AAACAGACAA CGCCACCCTG
    CTGCGCCTGT TGGAGGAGGG CGAAAAGATC CAGCACATGT ACCGCTGTGC TCGAGTCCAG
    GGCCTTGATA CCAGTGAGGG GCTACTCCTC TTTGGTAAAG AGCATTTCTA TGTGATTGAT
    GGATTTACAA TGACAGCAAC CAGAGAAATA AGAGATATTG AAACCTTACC TCCAAATATG
    CATGAGCCGA TTATCCCTAG AGGAGCCAGG CAAGGCCCCA GTCAGCTCAA GAGAACGTGC
    AGTATCTTTG CATATGAAGA TATCAAGGAA GTGCATAAAC GGAGATATCT CCTGCAGCCT
    ATCGCTGTGG AAGTTTTCTC TGGAGATGGA CGAAACTACC TCCTTGCTTT TCAGAAAGGA
    ATCAGAAACA AAGTTTATCA AAGGTTTTTG GCTGTAGTGC CATCTCTCAC TGACAGTTCA
    GAGTCTGTGT CTGGGCAGCG GCCGAACACC AGCGTGGAGC AGGGATCTGG GTTGCTTAGC
    ACTCTGGTTG GAGAGAAGTC TGTGACTCAG AGATGGGAGA GAGGTGAAAT CAGCAACTTT
    CAGTATTTGA TGCACTTGAA CACTTTGGCT GGAAGATCGT ATAATGACCT CATGCAATAT
    CCTGTCTTCC CCTGGATCCT TGCAGATTAC GACTCGGAGG AGGTCGATCT TACTAATCCC
    AAGACATTTA GAAACCTGGC CAAGCCAATG GGAGCACAGA CAGATGAACG ATTAGCTCAG
    TATAAGAAGC GATATAAAGA CTGGGAAGAT CCTAATGGAG AAACTCCAGC CTACCACTAT
    GGGACCCACT ATTCATCAGC CATGATTGTG GCTTCATACC TTGTCAGAAT GGAGCCCTTC
    ACACAGATAT TCCTAAGGCT ACAGGGAGGC CACTTTGACC TGGCTGACCG GATGTTTCAC
    AGCGTGCGTG AGGCCTGGTA CTCAGCGTCA AAGCACAACA TGGCAGATGT AAAGGAACTC
    ATCCCAGAAT TTTTTTACTT ACCAGAGTTC CTGTTCAATT CCAACAACTT TGACCTTGGC
    TGTAAACAAA ACGGCACCAA ACTTGGAGAT GTTATCCTTC CACCCTGGGC AAAAGGGGAC
    CCACGAGAAT TCATCCGGGT GCACCGTGAG GCTCTGGAAT GTGACTACGT GAGTGCCCAT
    CTGCATGAGT GGATCGACTT AATCTTCGGC TATAAGCAGC AAGGTCCTGC CGCAGTAGAA
    GCTGTAAACG TCTTCCATCA CCTTTTCTAT GAGGGTCAAG TGGATATCTA CAACATAAAT
    GACCCCCTCA AGGAAACAGC CACCATAGGG TTCATTAATA ACTTTGGGCA GATCCCTAAA
    CAGTTATTTA AAAAACCTCA TCCACCCAAG CGAGTGAGGA GTCGACTCAA CGGAGACAAT
    GCGGGGGCCT CCGTCCCGCC AGGGTCTGCC AGTGACAAGA TCTTTTTTCA TCATCTGGAC
    AACCTGAGGC CTTCACTAAC ACCTGTAAAA GAACTCAAAG AGCCTGTGGG ACAAATCGTG
    TGTACAGACA AAGGCATTCT TGCGGTAGAA CAGAATAAGG TTCTTATCCC ACCAACCTGG
    AACAAGACTT TCGCCTGGGG CTACGCAGAC CTCAGCTGCA GGCTGGGGAC CTATGAGTCA
    GACAAGGCAG TGACTGTTTA TGAATGTTTG TCGGAGTGGG GCCAGATTCT CTGTGCGATC
    TGCCCCAACC CCAAGCTGGT CATCACGGGT GGAACAAGTA CGGTGGTGTG TGTTTGGGAG
    ATGAGTACCT CGAAAGAAAA GGCTAAGACT CTCACTCTCA AACAGGCCTT ACTTGGCCAC
    ACGGATACGG TTACCTGTGC CACCGCGTCG TTAGCCTATC ACATCATCGT CAGCGGGTCC
    CGAGACCGAA CCTGCATCAT CTGGGACCTG AACAAGCTGT CATTCCTAAC CCAGCTCCGA
    GGCCACCGGG CTCCCGTGTC TGCTCTGTGT ATCAATGAAT TAACCGGGGA CATCGTGTCC
    TGCGCCGGCA CGTACATCCA TGTGTGGAGC ATAAACGGGA ACCCCGTCGT GAGTGTGAAC
    ACGTTCACAG GCAGGAGCCA GCAGATCGTC TGTTGCTGTG TGTCGGAGAT GAACGAGTGG
    GACACGCAGA ACGTCATCGT CACGGGACAC TCGGATGGCG TGGTCCGGTT TTGGAGAATG
    GAATTTTTGC AAGTTCCTGA AACACCAGCC CCTGAGCCTG CTGAAGTCCT CGAAATGCAG
    GAAGACTGTC CAGAAGCACA AATCGGGCAA GAAGCCCAGG ACGAGGACAG TAGCGATTCC
    GAGGCCGACG AGCAGAGCGT CAGCCAGGAC CCCAAGGACA CGCCCAGCCA GCCCAGCAGC
    ACCAGCCACC GGCCCCGGGC AGCCTCCTGC AGAGCCACAG CCACCTGGTA TAACGACAGT
    GGCTCCGACG ACTCCAGGCG CTGGTCCGAT CAGCTCAGCC TCGATGAGAA AGACGGCTTC
    ATATTTGTGA ACTATTCAGA GGGCCAGAGC AGACCTCATC TTCAGACTCC CCTTAACCAC
    CCCCACCCCA ATCCCATCGA GGCGCGGAAT TACAGCCGAT TGAAGCCTGG GTACCGCTGG
    GAACGGCAGC TCGTGTTCAG GAGTAAGCTG ACCATGCACA CGGCCTTTGA CCGGAAGGAC
    AATGCTCACC CTGCCGAGAT CACTGCATTG GGCGTCTCCA AGGACCACAG CAGGATCCTC
    GTGGGCGACA GTCGGGGCCG GGTGTTCAGC TGGTCTGTGA GCGACCAGCC CGGCCGCTCT
    GCCGCTGACC ACTGGGTGAA GGACGAAGGA GGGGACAGCT GTTCAGGCTG CTCCGTGCGG
    TTCTCTCTCA CGGAAAGACG GCACCATTGC AGGAACTGTG GTCAGCTCTT CTGCCAGAAG
    TGCAGCCGAT TTCAGTCAGA AATCAAGCGC TTGAAAATCT CCTCCCCAGT ACGTGTCTGT
    CAGAACTGTT ACTACAACTT ACAGCATGAG CGGAGTTCAG AAGACGGGCC TCGGAATTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327192.1
    CDS998..10780
    Translation

    Target ORF information:

    RefSeq Version XM_015471706.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471706.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGGC ACGCGTATCA TTCTGTTTCT ACTCCCCCCG TCTACCCTCC TAAAAACGTT
    GCTGACCTGA AACCACACGT GGCAACTTCA TCTCTCTCAA GTTCTGATGC CGTCATCATC
    CATCCTGGAG CCATGCTTGC TATGCTGGAT CTCCTGGCCT CTGTTGGGTC AGTGACACAG
    CCAGAACATG CCTTGGACCT TCAACTTGCC GTGGCCAATA TTTTACAATC CCTGGTGCAC
    ACGGAAAGAA ACCAGCAGGT CATGTGTGAA GCTGGTCTCC ATGCACGACT GCTGCAGCGG
    TGCAGTGCCG CTCTGGCTGA CGAGGACCAC TCGCTGCACC CACCCCTGCA GCGCATGTTC
    GAGCGATTAG CCTCTCAAGC GCTGGAGCCC ATGGTGCTCA GGGAGTTTTT GCGTTTGGCA
    AGTCCATTAA ATTGTGGTGC CTGGGACAAA AAACTGCTAA AACAATATAG GGTCCACAAA
    CCAAGTTCAC TGAGTTTTGA GCCAGAGATG AGAAGCAGTA TGATCACATC CATGGAAGGT
    CTGGGTTCTG ATAATGTTTT CAGCTTACAT GAAGATAACC ATTACCGGAT AAGCAAGAGC
    CTAGTGAAAT CTGCAGAAGG AAGTACTGTA CCCCTAACCA GAGTGAAATG TCTGGTCTCC
    ATGACAACCC CACATGACAT CAGACTTCAT GGGTCATCAG TTACTCCAGC TTTTGTTGAA
    TTTGACACAT CCCTTGAAGG GTTTGGCTGC CTGTTCCTGC CCAGTCTGGC CCCTCATAAT
    GCCCCTACAA ACAATGCTGT CACAACAGGT CTTATCGATG GGGCCGTGGT CAGCGGTATT
    GGTTCTGGTG AACGATTCTT CCCTCCTCCA TCTGGCTTAA GCTACTCCAG CTGGTTTTGC
    ATTGAACATT TTAGTTCTCC TCCCAGTAAC CACCCAGTCA GACTCCTTAC TGTTGTTCGC
    CGAGCAAATT CTTCTGAGCA CCATTATGTA TGCCTTGCCA TAGTTCTCTC TGCAAAAGAT
    CGGTCTCTGA TTGTCTCCAC CAAAGAGGAA CTACTCCAAA ATTATGTTGA TGATTTTAGT
    GAAGAATCCT CTTTCTATGA GATTCTGCCA TGCTGTGCTC GCTTCCGTTG TGGAGAACTG
    ATCGTTGAGG GACAGTGGCA TCACCTGGTT CTGGTGATGA GCAAAGGGAT GCTGAAGAAC
    AGCACTGCAA CCCTCTACAT CGACGGCCAG CTTGTCAGCA CTGTGAAGCT TCATTATGTT
    CACAGTACTC CCGGGGGCTC AGGCTCTGCA AACCCACCAG TGGTTAGCAC AGTCTATGCC
    TACATTGGTA CTCCGCCTGC CCAGCGCCAA ATTGCTTCAT TGGTTTGGCG CCTGGGACCT
    ACACACTTTC TAGAAGAAGT ATTACCTCCT TCAAATGTTA CTACCATTTA TGAACTAGGA
    CCAAATTACG TGGGAAGCTT TCAGGCCGTA TGTATGCCAT GTAAAGATGC AAAATCTGAA
    GGTGTGGTCC CATCTCCTGT GTCATTAGTA CCAGAGGAAA AGGTGTCATT CGGCCTCTAT
    GCACTGTCCG TGTCCGCCTT GACAGTGGCG AGAATCCGGA AGGTGTACAA CAAGCTGGAC
    AGCAAAGCCA TCGCTAAGCA GTTAGGCATT TCCTCCCATG AGAACGCCAC TCCTGTGAAG
    CTGATCCACA ACTCCGCAGG ACACCTCAAT GGGCCCGCAC GGACAATCGG GGCCAGTCTG
    ATTGGATACT TGGGAGTAAG AACATTTGTT CCTAAGCCTG TTGCCACTAC TTTGCAGTAC
    GTTGGTGGGG CCGCAGCCAT CCTGGGCCTG GTAGCCATGG CTTCTGATGT GGAAGGGCTG
    TATGCAGCGG TCAAGGCCCT AGTTTGTGTG GTCAAGAGTA ACCCACTAGC CAGCAAAGAA
    ATGGAAAGAA TCCGGGGCTA CCAGTTGCTG GCAATGCTGT TTAAGAAGAA ACGCTCCCTT
    CTTAACAGCC ACATTCTCCA TCTCACTTTT TCCTTGGTGG GAACTGTTGA CAGTGGACAT
    GAGACTTCCA TTATTCCAAA TTCAACAGCT TTCCAGGATC TACTTTGTGA TTTTGAAGTC
    TGGCTCCATG CTCCATATGA ACTTCATCTC TCCCTATTTG AACACTTTAT TGAATTGCTC
    ACAGAGTCCA GTGAAGCTTC AAAGAATGCC AAGTTGATGA GGGAATTCCA GCTAATCCCA
    AAGCTGCTAC TGACTCTTCG AGATATGTCT CTATCCCAGC CTACCATTGC TGCTATTAGT
    AACGTGCTGA GCTTCTTACT GCAGGGTTTT CCCAACAGCA ATGATCTACT CAGGTTTGGC
    CAGTTCATTT CTTCTACTTT ACCAACCTTT GCAGTTTGTG AGAAGTTTGT AGTTATGGAA
    ATAAACCACG AAGAGAAGCC TGACACTGGA ACTGAAGAAG AGTTTGGAGG TCTTGTATCT
    GCTAATCTTA TACTTTTGAG GAACAGACTT CTGGATATCT TACTAAAACT GGTTTACACA
    TCTAAAGAAA AGACAAGCAT TAATTTGCAA GCTTGTGAAG AGCTGGTCAG GACGCTGGGC
    TTTGACTGGA TTATGATGTT TATGGAAGAA CACTTGCATT CTACCACAGT CACAGCAGCC
    ATGAGGATTC TTGTTGTCCT ACTGAGTAAT CAGTCTATTC TCATCAAGTT CAAGGAGGGA
    CTCAGTGGTG GAGGCTGGCT GGAGCAGACA GATTCTGTCT TAACGAATAA GATCGGAACT
    GTATTAGGAT TCAATGTGGG CAGGAGTGCT GGCGGACGAT CAACTGTCAG GGAGATTAAC
    CGGGATGCTT GTCACTTTCC TGGCTTTCCG GTGCTTCAGT CATTCCTTCC TAAACACACT
    AATGTCCCTG CTCTCTATTT CCTCCTCATG GCATTGTTTC TGCAGCAGCC CGTTAGTGAG
    CTGCCTGAGA ACCTGCAGGT CAGTGTGCCT GTCGTCAGCA GCCGATGTAA GCAGGGTTGC
    CAGTTTGATT TAGATTCCAT CTGGACCTTT ATCTTTGGAG TTCCTGCCTC CAGTGGAACC
    GTGGTCTCAT CTATCCACAA TGTGTGCACA GAAGCTGCTT TTTTGTTACT GGGCATGCTC
    CGAAGCATGC TGAATTCACC TTGGCAATCA GAAGAAGAGG GGTCTTGGCT CCGGGAGTAT
    CCTGTGACCC TGATGCAGTT CTTCAGATAC TTGTATCACA ATGTCCCGGA CCTGGCCTCC
    ATGTGGATGA ACCCTGATTT CCTGTGTGCA CTTGCAGCCA CGGTCTTCCC CTTCAACATC
    CGCCCTTATT CAGAAATGGT GACTGATCTC GATGATGAAG TTGGATCTCC AGCTGAAGAA
    TTTAAAGCCT TTGCAGCAGA CACAGGGATG AACAGAAGCC AATCAGAGTA CTGCAACGTG
    GGCGTCAAGA CATACCTGAC TAATCACCCA GCTAAGAAAT TTGTTTTTGA CTTCATGCGG
    GTCTTAATAA TAGACAACCT CTGTCTTACA CCTGCCAGCA AGCAGACTCC ACTGATTGAT
    CTTTTGTTGG AGGCTTCTCC TGAGAGATCG ACAAGAACGC AGCAAAAAGA ATTTCAGACT
    TACATTTTGG ATAGTGTGAT GGATCATTTG CTTGCAGCTG ATGTTTTACT GGGAGAAGAT
    GCATCTCTTC CTATCACCAG TGGAGGAAGC TACCAGGTAT TGGTGAACAA TGTGTTTTAT
    TTCACACAAC GTGTGGTGGA CAAGCTTTGG CAAGGCATGT TCAACAAAGA ATCTAAACTT
    CTTATAGATT TTATAATTCA ACTAATTGCA CAGTCAAAGA GAAGATCCCA GGGGTTGTCA
    CTGGATGCAG TCTATCACTG CCTCAACAGG ACTATCCTGT ACCAGTTCTC CCGGGCGCAC
    AAAACCGTCC CGCAGCAAGT AGCCCTTCTT GACTCTCTCA GGGTCCTGAC TGTCAACAGG
    AACTTGATCC TGGGCCCCGG AAACCACGAC CAAGAATTCA TCAGCTGTCT GGCTCACTGC
    CTCATTAACC TGCATGTTGG GAGCAATGTG GATGGATTTG GACTGGAAGC AGAAGCCCGC
    ATGACTACAT GGCATATTAT GATCCCCTCG GACATTGAAC CAGATGGTGG TTACAGCCAA
    GATATTAGTG AAGGGCGTCA GCTTCTCATA AAAGCTGTCA ACAGAGTTTG GACGGAATTA
    ATTCATAGTA AGAAACAAGT CTTGGAGGAG CTTTTCAAAG TCACTCTACC TGTGAATGAA
    AGAGGCCACG TGGACATAGC TATAGCAAGG CCGCTCATCG AAGAAGCTAG TTTGAAGTGC
    TGGCAGAATC ATCTAGCCCA TGAAAAGAAG TGCATAAGTC GAGGAGAAGC TTTAATGCCC
    ACCACACAAT CCAAATTGTC CCGTGTCAGC AGTGGCTTTG GTCTTTCCAA GTTAACGGGA
    TCAAGAAGGA ATCGAAAGGA AAGCGGGCTT CATAAACACA GCCTTTCCAC CCAGGAGATT
    TCTCAGTGGA TGTTTACACA CATCGCTGTT GTTCGTGACT TGGTAGACAC ACAATATAAG
    GAATACCAGG AGCGTCAGCA AAATGCCCTG AAGTACGTGA CGGAAGAGTG GTGCCAGATC
    GAGTATGAGC TGCTGCGGGA GCGGGGACTC TGGGGCCCCC CCATTGGTTC CCATCTCGAC
    AAGTGGATGT TGGAGATGAC AGAGGGTCCC TGCAGAATGA GGAAAAAAAT GGTGCGAAAT
    GATATGTTTT ATAACCATTA CCCTTACGTG CCAGAAACGG AGCAGGAAAC CAACGTGGCG
    TCTGAGATCC CAAGTAAACA GCCTGAGACA CCTGATGATA TCATTCCTCA AAAGAAGCCT
    GCTCGATACA GAAGAGCCGT AAGTTATGAC AGTAAAGAAT ACTACATGCG ACTAGCCTCC
    GGCAACCCAG CCATTGTGCA GGATGCCATC GTAGAGAGCT CTGAAGGAGA AGCTGCTCAG
    CAAGAACCAG AACATGGTGA AGACACGATT GCTAAAGTCA AAGGACTGGT CAAGCCACCT
    CTCAAACGGT CTCGGTCAGC ACCGGACGGA GGCGATGAGG AGACACAGGA GCAGTTACAG
    GACCAGACAG CTGAGGGCAG CTCGATAGAA GAGGAGGAGA AAACAGACAA CGCCACCCTG
    CTGCGCCTGT TGGAGGAGGG CGAAAAGATC CAGCACATGT ACCGCTGTGC TCGAGTCCAG
    GGCCTTGATA CCAGTGAGGG GCTACTCCTC TTTGGTAAAG AGCATTTCTA TGTGATTGAT
    GGATTTACAA TGACAGCAAC CAGAGAAATA AGAGATATTG AAACCTTACC TCCAAATATG
    CATGAGCCGA TTATCCCTAG AGGAGCCAGG CAAGGCCCCA GTCAGCTCAA GAGAACGTGC
    AGTATCTTTG CATATGAAGA TATCAAGGAA GTGCATAAAC GGAGATATCT CCTGCAGCCT
    ATCGCTGTGG AAGTTTTCTC TGGAGATGGA CGAAACTACC TCCTTGCTTT TCAGAAAGGA
    ATCAGAAACA AAGTTTATCA AAGGTTTTTG GCTGTAGTGC CATCTCTCAC TGACAGTTCA
    GAGTCTGTGT CTGGGCAGCG GCCGAACACC AGCGTGGAGC AGGGATCTGG GTTGCTTAGC
    ACTCTGGTTG GAGAGAAGTC TGTGACTCAG AGATGGGAGA GAGGTGAAAT CAGCAACTTT
    CAGTATTTGA TGCACTTGAA CACTTTGGCT GGAAGATCGT ATAATGACCT CATGCAATAT
    CCTGTCTTCC CCTGGATCCT TGCAGATTAC GACTCGGAGG AGGTCGATCT TACTAATCCC
    AAGACATTTA GAAACCTGGC CAAGCCAATG GGAGCACAGA CAGATGAACG ATTAGCTCAG
    TATAAGAAGC GATATAAAGA CTGGGAAGAT CCTAATGGAG AAACTCCAGC CTACCACTAT
    GGGACCCACT ATTCATCAGC CATGATTGTG GCTTCATACC TTGTCAGAAT GGAGCCCTTC
    ACACAGATAT TCCTAAGGCT ACAGGGAGGC CACTTTGACC TGGCTGACCG GATGTTTCAC
    AGCGTGCGTG AGGCCTGGTA CTCAGCGTCA AAGCACAACA TGGCAGATGT AAAGGAACTC
    ATCCCAGAAT TTTTTTACTT ACCAGAGTTC CTGTTCAATT CCAACAACTT TGACCTTGGC
    TGTAAACAAA ACGGCACCAA ACTTGGAGAT GTTATCCTTC CACCCTGGGC AAAAGGGGAC
    CCACGAGAAT TCATCCGGGT GCACCGTGAG GCTCTGGAAT GTGACTACGT GAGTGCCCAT
    CTGCATGAGT GGATCGACTT AATCTTCGGC TATAAGCAGC AAGGTCCTGC CGCAGTAGAA
    GCTGTAAACG TCTTCCATCA CCTTTTCTAT GAGGGTCAAG TGGATATCTA CAACATAAAT
    GACCCCCTCA AGGAAACAGC CACCATAGGG TTCATTAATA ACTTTGGGCA GATCCCTAAA
    CAGTTATTTA AAAAACCTCA TCCACCCAAG CGAGTGAGGA GTCGACTCAA CGGAGACAAT
    GCGGGGGCCT CCGTCCCGCC AGGGTCTGCC AGTGACAAGA TCTTTTTTCA TCATCTGGAC
    AACCTGAGGC CTTCACTAAC ACCTGTAAAA GAACTCAAAG AGCCTGTGGG ACAAATCGTG
    TGTACAGACA AAGGCATTCT TGCGGTAGAA CAGAATAAGG TTCTTATCCC ACCAACCTGG
    AACAAGACTT TCGCCTGGGG CTACGCAGAC CTCAGCTGCA GGCTGGGGAC CTATGAGTCA
    GACAAGGCAG TGACTGTTTA TGAATGTTTG TCGGAGTGGG GCCAGATTCT CTGTGCGATC
    TGCCCCAACC CCAAGCTGGT CATCACGGGT GGAACAAGTA CGGTGGTGTG TGTTTGGGAG
    ATGAGTACCT CGAAAGAAAA GGCTAAGACT CTCACTCTCA AACAGGCCTT ACTTGGCCAC
    ACGGATACGG TTACCTGTGC CACCGCGTCG TTAGCCTATC ACATCATCGT CAGCGGGTCC
    CGAGACCGAA CCTGCATCAT CTGGGACCTG AACAAGCTGT CATTCCTAAC CCAGCTCCGA
    GGCCACCGGG CTCCCGTGTC TGCTCTGTGT ATCAATGAAT TAACCGGGGA CATCGTGTCC
    TGCGCCGGCA CGTACATCCA TGTGTGGAGC ATAAACGGGA ACCCCGTCGT GAGTGTGAAC
    ACGTTCACAG GCAGGAGCCA GCAGATCGTC TGTTGCTGTG TGTCGGAGAT GAACGAGTGG
    GACACGCAGA ACGTCATCGT CACGGGACAC TCGGATGGCG TGGTCCGGTT TTGGAGAATG
    GAATTTTTGC AAGTTCCTGA AACACCAGCC CCTGAGCCTG CTGAAGTCCT CGAAATGCAG
    GAAGACTGTC CAGAAGCACA AATCGGGCAA GAAGCCCAGG ACGAGGACAG TAGCGATTCC
    GAGGCCGACG AGCAGAGCGT CAGCCAGGAC CCCAAGGACA CGCCCAGCCA GCCCAGCAGC
    ACCAGCCACC GGCCCCGGGC AGCCTCCTGC AGAGCCACAG CCACCTGGTA TAACGACAGT
    GGCTCCGACG ACTCCAGGCG CTGGTCCGAT CAGCTCAGCC TCGATGAGAA AGACGGCTTC
    ATATTTGTGA ACTATTCAGA GGGCCAGAGC AGACCTCATC TTCAGACTCC CCTTAACCAC
    CCCCACCCCA ATCCCATCGA GGCGCGGAAT TACAGCCGAT TGAAGCCTGG GTACCGCTGG
    GAACGGCAGC TCGTGTTCAG GAGTAAGCTG ACCATGCACA CGGCCTTTGA CCGGAAGGAC
    AATGCTCACC CTGCCGAGAT CACTGCATTG GGCGTCTCCA AGGACCACAG CAGGATCCTC
    GTGGGCGACA GTCGGGGCCG GGTGTTCAGC TGGTCTGTGA GCGACCAGCC CGGCCGCTCT
    GCCGCTGACC ACTGGGTGAA GGACGAAGGA GGGGACAGCT GTTCAGGCTG CTCCGTGCGG
    TTCTCTCTCA CGGAAAGACG GCACCATTGC AGGAACTGTG GTCAGCTCTT CTGCCAGAAG
    TGCAGCCGAT TTCAGTCAGA AATCAAGCGC TTGAAAATCT CCTCCCCAGT ACGTGTCTGT
    CAGAACTGTT ACTACAACTT ACAGCATGAG CGGAGTTCAG AAGACGGGCC TCGGAATTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90117
    Clone ID Related Accession (Same CDS sequence) XM_015471706.1 , XM_015463826.1
    Accession Version XM_015463826.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product WD repeat and FYVE domain-containing protein 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644958.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGGC ACGCGTATCA TTCTGTTTCT ACTCCCCCCG TCTACCCTCC TAAAAACGTT
    GCTGACCTGA AACCACACGT GGCAACTTCA TCTCTCTCAA GTTCTGATGC CGTCATCATC
    CATCCTGGAG CCATGCTTGC TATGCTGGAT CTCCTGGCCT CTGTTGGGTC AGTGACACAG
    CCAGAACATG CCTTGGACCT TCAACTTGCC GTGGCCAATA TTTTACAATC CCTGGTGCAC
    ACGGAAAGAA ACCAGCAGGT CATGTGTGAA GCTGGTCTCC ATGCACGACT GCTGCAGCGG
    TGCAGTGCCG CTCTGGCTGA CGAGGACCAC TCGCTGCACC CACCCCTGCA GCGCATGTTC
    GAGCGATTAG CCTCTCAAGC GCTGGAGCCC ATGGTGCTCA GGGAGTTTTT GCGTTTGGCA
    AGTCCATTAA ATTGTGGTGC CTGGGACAAA AAACTGCTAA AACAATATAG GGTCCACAAA
    CCAAGTTCAC TGAGTTTTGA GCCAGAGATG AGAAGCAGTA TGATCACATC CATGGAAGGT
    CTGGGTTCTG ATAATGTTTT CAGCTTACAT GAAGATAACC ATTACCGGAT AAGCAAGAGC
    CTAGTGAAAT CTGCAGAAGG AAGTACTGTA CCCCTAACCA GAGTGAAATG TCTGGTCTCC
    ATGACAACCC CACATGACAT CAGACTTCAT GGGTCATCAG TTACTCCAGC TTTTGTTGAA
    TTTGACACAT CCCTTGAAGG GTTTGGCTGC CTGTTCCTGC CCAGTCTGGC CCCTCATAAT
    GCCCCTACAA ACAATGCTGT CACAACAGGT CTTATCGATG GGGCCGTGGT CAGCGGTATT
    GGTTCTGGTG AACGATTCTT CCCTCCTCCA TCTGGCTTAA GCTACTCCAG CTGGTTTTGC
    ATTGAACATT TTAGTTCTCC TCCCAGTAAC CACCCAGTCA GACTCCTTAC TGTTGTTCGC
    CGAGCAAATT CTTCTGAGCA CCATTATGTA TGCCTTGCCA TAGTTCTCTC TGCAAAAGAT
    CGGTCTCTGA TTGTCTCCAC CAAAGAGGAA CTACTCCAAA ATTATGTTGA TGATTTTAGT
    GAAGAATCCT CTTTCTATGA GATTCTGCCA TGCTGTGCTC GCTTCCGTTG TGGAGAACTG
    ATCGTTGAGG GACAGTGGCA TCACCTGGTT CTGGTGATGA GCAAAGGGAT GCTGAAGAAC
    AGCACTGCAA CCCTCTACAT CGACGGCCAG CTTGTCAGCA CTGTGAAGCT TCATTATGTT
    CACAGTACTC CCGGGGGCTC AGGCTCTGCA AACCCACCAG TGGTTAGCAC AGTCTATGCC
    TACATTGGTA CTCCGCCTGC CCAGCGCCAA ATTGCTTCAT TGGTTTGGCG CCTGGGACCT
    ACACACTTTC TAGAAGAAGT ATTACCTCCT TCAAATGTTA CTACCATTTA TGAACTAGGA
    CCAAATTACG TGGGAAGCTT TCAGGCCGTA TGTATGCCAT GTAAAGATGC AAAATCTGAA
    GGTGTGGTCC CATCTCCTGT GTCATTAGTA CCAGAGGAAA AGGTGTCATT CGGCCTCTAT
    GCACTGTCCG TGTCCGCCTT GACAGTGGCG AGAATCCGGA AGGTGTACAA CAAGCTGGAC
    AGCAAAGCCA TCGCTAAGCA GTTAGGCATT TCCTCCCATG AGAACGCCAC TCCTGTGAAG
    CTGATCCACA ACTCCGCAGG ACACCTCAAT GGGCCCGCAC GGACAATCGG GGCCAGTCTG
    ATTGGATACT TGGGAGTAAG AACATTTGTT CCTAAGCCTG TTGCCACTAC TTTGCAGTAC
    GTTGGTGGGG CCGCAGCCAT CCTGGGCCTG GTAGCCATGG CTTCTGATGT GGAAGGGCTG
    TATGCAGCGG TCAAGGCCCT AGTTTGTGTG GTCAAGAGTA ACCCACTAGC CAGCAAAGAA
    ATGGAAAGAA TCCGGGGCTA CCAGTTGCTG GCAATGCTGT TTAAGAAGAA ACGCTCCCTT
    CTTAACAGCC ACATTCTCCA TCTCACTTTT TCCTTGGTGG GAACTGTTGA CAGTGGACAT
    GAGACTTCCA TTATTCCAAA TTCAACAGCT TTCCAGGATC TACTTTGTGA TTTTGAAGTC
    TGGCTCCATG CTCCATATGA ACTTCATCTC TCCCTATTTG AACACTTTAT TGAATTGCTC
    ACAGAGTCCA GTGAAGCTTC AAAGAATGCC AAGTTGATGA GGGAATTCCA GCTAATCCCA
    AAGCTGCTAC TGACTCTTCG AGATATGTCT CTATCCCAGC CTACCATTGC TGCTATTAGT
    AACGTGCTGA GCTTCTTACT GCAGGGTTTT CCCAACAGCA ATGATCTACT CAGGTTTGGC
    CAGTTCATTT CTTCTACTTT ACCAACCTTT GCAGTTTGTG AGAAGTTTGT AGTTATGGAA
    ATAAACCACG AAGAGAAGCC TGACACTGGA ACTGAAGAAG AGTTTGGAGG TCTTGTATCT
    GCTAATCTTA TACTTTTGAG GAACAGACTT CTGGATATCT TACTAAAACT GGTTTACACA
    TCTAAAGAAA AGACAAGCAT TAATTTGCAA GCTTGTGAAG AGCTGGTCAG GACGCTGGGC
    TTTGACTGGA TTATGATGTT TATGGAAGAA CACTTGCATT CTACCACAGT CACAGCAGCC
    ATGAGGATTC TTGTTGTCCT ACTGAGTAAT CAGTCTATTC TCATCAAGTT CAAGGAGGGA
    CTCAGTGGTG GAGGCTGGCT GGAGCAGACA GATTCTGTCT TAACGAATAA GATCGGAACT
    GTATTAGGAT TCAATGTGGG CAGGAGTGCT GGCGGACGAT CAACTGTCAG GGAGATTAAC
    CGGGATGCTT GTCACTTTCC TGGCTTTCCG GTGCTTCAGT CATTCCTTCC TAAACACACT
    AATGTCCCTG CTCTCTATTT CCTCCTCATG GCATTGTTTC TGCAGCAGCC CGTTAGTGAG
    CTGCCTGAGA ACCTGCAGGT CAGTGTGCCT GTCGTCAGCA GCCGATGTAA GCAGGGTTGC
    CAGTTTGATT TAGATTCCAT CTGGACCTTT ATCTTTGGAG TTCCTGCCTC CAGTGGAACC
    GTGGTCTCAT CTATCCACAA TGTGTGCACA GAAGCTGCTT TTTTGTTACT GGGCATGCTC
    CGAAGCATGC TGAATTCACC TTGGCAATCA GAAGAAGAGG GGTCTTGGCT CCGGGAGTAT
    CCTGTGACCC TGATGCAGTT CTTCAGATAC TTGTATCACA ATGTCCCGGA CCTGGCCTCC
    ATGTGGATGA ACCCTGATTT CCTGTGTGCA CTTGCAGCCA CGGTCTTCCC CTTCAACATC
    CGCCCTTATT CAGAAATGGT GACTGATCTC GATGATGAAG TTGGATCTCC AGCTGAAGAA
    TTTAAAGCCT TTGCAGCAGA CACAGGGATG AACAGAAGCC AATCAGAGTA CTGCAACGTG
    GGCGTCAAGA CATACCTGAC TAATCACCCA GCTAAGAAAT TTGTTTTTGA CTTCATGCGG
    GTCTTAATAA TAGACAACCT CTGTCTTACA CCTGCCAGCA AGCAGACTCC ACTGATTGAT
    CTTTTGTTGG AGGCTTCTCC TGAGAGATCG ACAAGAACGC AGCAAAAAGA ATTTCAGACT
    TACATTTTGG ATAGTGTGAT GGATCATTTG CTTGCAGCTG ATGTTTTACT GGGAGAAGAT
    GCATCTCTTC CTATCACCAG TGGAGGAAGC TACCAGGTAT TGGTGAACAA TGTGTTTTAT
    TTCACACAAC GTGTGGTGGA CAAGCTTTGG CAAGGCATGT TCAACAAAGA ATCTAAACTT
    CTTATAGATT TTATAATTCA ACTAATTGCA CAGTCAAAGA GAAGATCCCA GGGGTTGTCA
    CTGGATGCAG TCTATCACTG CCTCAACAGG ACTATCCTGT ACCAGTTCTC CCGGGCGCAC
    AAAACCGTCC CGCAGCAAGT AGCCCTTCTT GACTCTCTCA GGGTCCTGAC TGTCAACAGG
    AACTTGATCC TGGGCCCCGG AAACCACGAC CAAGAATTCA TCAGCTGTCT GGCTCACTGC
    CTCATTAACC TGCATGTTGG GAGCAATGTG GATGGATTTG GACTGGAAGC AGAAGCCCGC
    ATGACTACAT GGCATATTAT GATCCCCTCG GACATTGAAC CAGATGGTGG TTACAGCCAA
    GATATTAGTG AAGGGCGTCA GCTTCTCATA AAAGCTGTCA ACAGAGTTTG GACGGAATTA
    ATTCATAGTA AGAAACAAGT CTTGGAGGAG CTTTTCAAAG TCACTCTACC TGTGAATGAA
    AGAGGCCACG TGGACATAGC TATAGCAAGG CCGCTCATCG AAGAAGCTAG TTTGAAGTGC
    TGGCAGAATC ATCTAGCCCA TGAAAAGAAG TGCATAAGTC GAGGAGAAGC TTTAATGCCC
    ACCACACAAT CCAAATTGTC CCGTGTCAGC AGTGGCTTTG GTCTTTCCAA GTTAACGGGA
    TCAAGAAGGA ATCGAAAGGA AAGCGGGCTT CATAAACACA GCCTTTCCAC CCAGGAGATT
    TCTCAGTGGA TGTTTACACA CATCGCTGTT GTTCGTGACT TGGTAGACAC ACAATATAAG
    GAATACCAGG AGCGTCAGCA AAATGCCCTG AAGTACGTGA CGGAAGAGTG GTGCCAGATC
    GAGTATGAGC TGCTGCGGGA GCGGGGACTC TGGGGCCCCC CCATTGGTTC CCATCTCGAC
    AAGTGGATGT TGGAGATGAC AGAGGGTCCC TGCAGAATGA GGAAAAAAAT GGTGCGAAAT
    GATATGTTTT ATAACCATTA CCCTTACGTG CCAGAAACGG AGCAGGAAAC CAACGTGGCG
    TCTGAGATCC CAAGTAAACA GCCTGAGACA CCTGATGATA TCATTCCTCA AAAGAAGCCT
    GCTCGATACA GAAGAGCCGT AAGTTATGAC AGTAAAGAAT ACTACATGCG ACTAGCCTCC
    GGCAACCCAG CCATTGTGCA GGATGCCATC GTAGAGAGCT CTGAAGGAGA AGCTGCTCAG
    CAAGAACCAG AACATGGTGA AGACACGATT GCTAAAGTCA AAGGACTGGT CAAGCCACCT
    CTCAAACGGT CTCGGTCAGC ACCGGACGGA GGCGATGAGG AGACACAGGA GCAGTTACAG
    GACCAGACAG CTGAGGGCAG CTCGATAGAA GAGGAGGAGA AAACAGACAA CGCCACCCTG
    CTGCGCCTGT TGGAGGAGGG CGAAAAGATC CAGCACATGT ACCGCTGTGC TCGAGTCCAG
    GGCCTTGATA CCAGTGAGGG GCTACTCCTC TTTGGTAAAG AGCATTTCTA TGTGATTGAT
    GGATTTACAA TGACAGCAAC CAGAGAAATA AGAGATATTG AAACCTTACC TCCAAATATG
    CATGAGCCGA TTATCCCTAG AGGAGCCAGG CAAGGCCCCA GTCAGCTCAA GAGAACGTGC
    AGTATCTTTG CATATGAAGA TATCAAGGAA GTGCATAAAC GGAGATATCT CCTGCAGCCT
    ATCGCTGTGG AAGTTTTCTC TGGAGATGGA CGAAACTACC TCCTTGCTTT TCAGAAAGGA
    ATCAGAAACA AAGTTTATCA AAGGTTTTTG GCTGTAGTGC CATCTCTCAC TGACAGTTCA
    GAGTCTGTGT CTGGGCAGCG GCCGAACACC AGCGTGGAGC AGGGATCTGG GTTGCTTAGC
    ACTCTGGTTG GAGAGAAGTC TGTGACTCAG AGATGGGAGA GAGGTGAAAT CAGCAACTTT
    CAGTATTTGA TGCACTTGAA CACTTTGGCT GGAAGATCGT ATAATGACCT CATGCAATAT
    CCTGTCTTCC CCTGGATCCT TGCAGATTAC GACTCGGAGG AGGTCGATCT TACTAATCCC
    AAGACATTTA GAAACCTGGC CAAGCCAATG GGAGCACAGA CAGATGAACG ATTAGCTCAG
    TATAAGAAGC GATATAAAGA CTGGGAAGAT CCTAATGGAG AAACTCCAGC CTACCACTAT
    GGGACCCACT ATTCATCAGC CATGATTGTG GCTTCATACC TTGTCAGAAT GGAGCCCTTC
    ACACAGATAT TCCTAAGGCT ACAGGGAGGC CACTTTGACC TGGCTGACCG GATGTTTCAC
    AGCGTGCGTG AGGCCTGGTA CTCAGCGTCA AAGCACAACA TGGCAGATGT AAAGGAACTC
    ATCCCAGAAT TTTTTTACTT ACCAGAGTTC CTGTTCAATT CCAACAACTT TGACCTTGGC
    TGTAAACAAA ACGGCACCAA ACTTGGAGAT GTTATCCTTC CACCCTGGGC AAAAGGGGAC
    CCACGAGAAT TCATCCGGGT GCACCGTGAG GCTCTGGAAT GTGACTACGT GAGTGCCCAT
    CTGCATGAGT GGATCGACTT AATCTTCGGC TATAAGCAGC AAGGTCCTGC CGCAGTAGAA
    GCTGTAAACG TCTTCCATCA CCTTTTCTAT GAGGGTCAAG TGGATATCTA CAACATAAAT
    GACCCCCTCA AGGAAACAGC CACCATAGGG TTCATTAATA ACTTTGGGCA GATCCCTAAA
    CAGTTATTTA AAAAACCTCA TCCACCCAAG CGAGTGAGGA GTCGACTCAA CGGAGACAAT
    GCGGGGGCCT CCGTCCCGCC AGGGTCTGCC AGTGACAAGA TCTTTTTTCA TCATCTGGAC
    AACCTGAGGC CTTCACTAAC ACCTGTAAAA GAACTCAAAG AGCCTGTGGG ACAAATCGTG
    TGTACAGACA AAGGCATTCT TGCGGTAGAA CAGAATAAGG TTCTTATCCC ACCAACCTGG
    AACAAGACTT TCGCCTGGGG CTACGCAGAC CTCAGCTGCA GGCTGGGGAC CTATGAGTCA
    GACAAGGCAG TGACTGTTTA TGAATGTTTG TCGGAGTGGG GCCAGATTCT CTGTGCGATC
    TGCCCCAACC CCAAGCTGGT CATCACGGGT GGAACAAGTA CGGTGGTGTG TGTTTGGGAG
    ATGAGTACCT CGAAAGAAAA GGCTAAGACT CTCACTCTCA AACAGGCCTT ACTTGGCCAC
    ACGGATACGG TTACCTGTGC CACCGCGTCG TTAGCCTATC ACATCATCGT CAGCGGGTCC
    CGAGACCGAA CCTGCATCAT CTGGGACCTG AACAAGCTGT CATTCCTAAC CCAGCTCCGA
    GGCCACCGGG CTCCCGTGTC TGCTCTGTGT ATCAATGAAT TAACCGGGGA CATCGTGTCC
    TGCGCCGGCA CGTACATCCA TGTGTGGAGC ATAAACGGGA ACCCCGTCGT GAGTGTGAAC
    ACGTTCACAG GCAGGAGCCA GCAGATCGTC TGTTGCTGTG TGTCGGAGAT GAACGAGTGG
    GACACGCAGA ACGTCATCGT CACGGGACAC TCGGATGGCG TGGTCCGGTT TTGGAGAATG
    GAATTTTTGC AAGTTCCTGA AACACCAGCC CCTGAGCCTG CTGAAGTCCT CGAAATGCAG
    GAAGACTGTC CAGAAGCACA AATCGGGCAA GAAGCCCAGG ACGAGGACAG TAGCGATTCC
    GAGGCCGACG AGCAGAGCGT CAGCCAGGAC CCCAAGGACA CGCCCAGCCA GCCCAGCAGC
    ACCAGCCACC GGCCCCGGGC AGCCTCCTGC AGAGCCACAG CCACCTGGTA TAACGACAGT
    GGCTCCGACG ACTCCAGGCG CTGGTCCGAT CAGCTCAGCC TCGATGAGAA AGACGGCTTC
    ATATTTGTGA ACTATTCAGA GGGCCAGAGC AGACCTCATC TTCAGACTCC CCTTAACCAC
    CCCCACCCCA ATCCCATCGA GGCGCGGAAT TACAGCCGAT TGAAGCCTGG GTACCGCTGG
    GAACGGCAGC TCGTGTTCAG GAGTAAGCTG ACCATGCACA CGGCCTTTGA CCGGAAGGAC
    AATGCTCACC CTGCCGAGAT CACTGCATTG GGCGTCTCCA AGGACCACAG CAGGATCCTC
    GTGGGCGACA GTCGGGGCCG GGTGTTCAGC TGGTCTGTGA GCGACCAGCC CGGCCGCTCT
    GCCGCTGACC ACTGGGTGAA GGACGAAGGA GGGGACAGCT GTTCAGGCTG CTCCGTGCGG
    TTCTCTCTCA CGGAAAGACG GCACCATTGC AGGAACTGTG GTCAGCTCTT CTGCCAGAAG
    TGCAGCCGAT TTCAGTCAGA AATCAAGCGC TTGAAAATCT CCTCCCCAGT ACGTGTCTGT
    CAGAACTGTT ACTACAACTT ACAGCATGAG CGGAGTTCAG AAGACGGGCC TCGGAATTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319312.1
    CDS998..10780
    Translation

    Target ORF information:

    RefSeq Version XM_015463826.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WD repeat and FYVE domain containing 3 (WDFY3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463826.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    ATGACCATAT CCCGTCATGG TCTCAGTGTC AACGTGGTGA AGTATATTCA CGAAAAAGAA 
    TGTTTATCTA CGTGTGTTCA GAATATGCAG CAGTCAGATG ACCTGTCTCC CCTAGAAATT
    GTTGAGATGT TTGCTGGGCT TTCTTGTTTC CTCAAAGATT CCAGTGATGT TTCCCAAACT
    CTTCTGGATG ATTTTCGGAT ATGGCAAGGA TATAATTTCC TTTGTGATCT CTTGCTTAGA
    TTGGAACAAG CAAAAGAGGC AGAATCCAAA GATGCCTTGA AAGATCTGGT TAATCTGATA
    ACTTCCCTAA CAACATATGG TGTCAATGAA CTAAAACCAG CTGGTATTAC CACAGGGGCA
    CCCTTTTTAT TGCCTGGATT TGCAGTACCT CAGCCTGCGG GCAAAGGTCA CAGTGTGAGA
    AACATCCAGG CCTTTGCAGT TCTTCAAAAT GCATTTTTAA AAGCAAAAAC CAACTTCCTC
    GCCCACATCA TTCTCGATGC CATCACAAAT ATTTACATGG CTGACAACGC CAACTATTTC
    ATCCTCGAAT CACAGCACAC ACTGTCACAG TTTGCAGAAA AAATTTCTAA ACTCCCAGAA
    GTACAAAACA AGTACTTCGA GATGCTGGAG TTTGTTGTTT TTAGCTTAAA TTATATCCCT
    TGTAAAGAAC TTATTAGTGT TAGCATCCTC TTAAAGTCTA GCTCTTCTTA TCACTGTAGC
    ATTATCGCGA TGAAAACACT TCTTAAGTTT ACACGACATG ACTACATATT TAAAGATGTG
    TTCAGGGAGG TGGGCCTTTT GGAGGTCATG GTAAACCTTT TGCACAAATA TGCTGCCCTC
    TTGAAGGATC CAACTCAGGC ACTAAATGAA CAAGGGGACT CAAGAAATAA TAGTTCATTT
    GAAGACCAAA AACACCTGGC TTTGTTAGTC ATGGAGACTT TGACAGTGCT TCTTCAAGGG
    TCAAACACAA ACGCAGGAAT TTTCCGAGAA TTTGGAGGCG CAAGATGTGC GCATAATATA
    GTGAGGTACC CACAGTGTCG GCAGCATGCC CTGATGATGA TCCAGCAGTT GGTGCTGTCT
    CCCAATGGGG ACGATGACAT GGGCACGCTT CTGGGACTCA TGCACTCGGC CCCACCCACA
    GAGCTGCAGC TGAAGACCGA CATCCTCAGA GCCCTCCTGT CGGTCCTTCG AGAAAGTCAC
    CGTTCCAGAA CAGTCTTTAG GAAGGTTGGA GGCTTTGTGT ACGTTACGTC CTTGCTCGTG
    GCTATGGAAA GGTCTTTGAG TGCGCCCCCC AAGAATGGGT GGGAGAAAGT GAGCCAGGGC
    CACGTGTTTG AACTTCTCCA CACCGTGTTC TGCACACTGA CTGCAGCGAT GCGTTATGAG
    CCAGCCAACT CACACTTCTT CAAAACAGAG ATTCAGTATG AGAAGCTGGC AGACGCTGTT
    CGATTTCTTG GCTGCTTCTC AGACCTAAGA AAGATCAGCC CCATGAATGT CTTCCCTTCA
    AATACTCAGC CATTTCAAAG ACTTTTAGAG GAAGACGTCA TCTCGATGGA CTCGGTGTCA
    CCCACTCTGC GGCACTGCAG TAAACTCTTT ATTTATCTCT ACAGAGTAGC CACAGATTCT
    TTTGACAGGC ACGCGTATCA TTCTGTTTCT ACTCCCCCCG TCTACCCTCC TAAAAACGTT
    GCTGACCTGA AACCACACGT GGCAACTTCA TCTCTCTCAA GTTCTGATGC CGTCATCATC
    CATCCTGGAG CCATGCTTGC TATGCTGGAT CTCCTGGCCT CTGTTGGGTC AGTGACACAG
    CCAGAACATG CCTTGGACCT TCAACTTGCC GTGGCCAATA TTTTACAATC CCTGGTGCAC
    ACGGAAAGAA ACCAGCAGGT CATGTGTGAA GCTGGTCTCC ATGCACGACT GCTGCAGCGG
    TGCAGTGCCG CTCTGGCTGA CGAGGACCAC TCGCTGCACC CACCCCTGCA GCGCATGTTC
    GAGCGATTAG CCTCTCAAGC GCTGGAGCCC ATGGTGCTCA GGGAGTTTTT GCGTTTGGCA
    AGTCCATTAA ATTGTGGTGC CTGGGACAAA AAACTGCTAA AACAATATAG GGTCCACAAA
    CCAAGTTCAC TGAGTTTTGA GCCAGAGATG AGAAGCAGTA TGATCACATC CATGGAAGGT
    CTGGGTTCTG ATAATGTTTT CAGCTTACAT GAAGATAACC ATTACCGGAT AAGCAAGAGC
    CTAGTGAAAT CTGCAGAAGG AAGTACTGTA CCCCTAACCA GAGTGAAATG TCTGGTCTCC
    ATGACAACCC CACATGACAT CAGACTTCAT GGGTCATCAG TTACTCCAGC TTTTGTTGAA
    TTTGACACAT CCCTTGAAGG GTTTGGCTGC CTGTTCCTGC CCAGTCTGGC CCCTCATAAT
    GCCCCTACAA ACAATGCTGT CACAACAGGT CTTATCGATG GGGCCGTGGT CAGCGGTATT
    GGTTCTGGTG AACGATTCTT CCCTCCTCCA TCTGGCTTAA GCTACTCCAG CTGGTTTTGC
    ATTGAACATT TTAGTTCTCC TCCCAGTAAC CACCCAGTCA GACTCCTTAC TGTTGTTCGC
    CGAGCAAATT CTTCTGAGCA CCATTATGTA TGCCTTGCCA TAGTTCTCTC TGCAAAAGAT
    CGGTCTCTGA TTGTCTCCAC CAAAGAGGAA CTACTCCAAA ATTATGTTGA TGATTTTAGT
    GAAGAATCCT CTTTCTATGA GATTCTGCCA TGCTGTGCTC GCTTCCGTTG TGGAGAACTG
    ATCGTTGAGG GACAGTGGCA TCACCTGGTT CTGGTGATGA GCAAAGGGAT GCTGAAGAAC
    AGCACTGCAA CCCTCTACAT CGACGGCCAG CTTGTCAGCA CTGTGAAGCT TCATTATGTT
    CACAGTACTC CCGGGGGCTC AGGCTCTGCA AACCCACCAG TGGTTAGCAC AGTCTATGCC
    TACATTGGTA CTCCGCCTGC CCAGCGCCAA ATTGCTTCAT TGGTTTGGCG CCTGGGACCT
    ACACACTTTC TAGAAGAAGT ATTACCTCCT TCAAATGTTA CTACCATTTA TGAACTAGGA
    CCAAATTACG TGGGAAGCTT TCAGGCCGTA TGTATGCCAT GTAAAGATGC AAAATCTGAA
    GGTGTGGTCC CATCTCCTGT GTCATTAGTA CCAGAGGAAA AGGTGTCATT CGGCCTCTAT
    GCACTGTCCG TGTCCGCCTT GACAGTGGCG AGAATCCGGA AGGTGTACAA CAAGCTGGAC
    AGCAAAGCCA TCGCTAAGCA GTTAGGCATT TCCTCCCATG AGAACGCCAC TCCTGTGAAG
    CTGATCCACA ACTCCGCAGG ACACCTCAAT GGGCCCGCAC GGACAATCGG GGCCAGTCTG
    ATTGGATACT TGGGAGTAAG AACATTTGTT CCTAAGCCTG TTGCCACTAC TTTGCAGTAC
    GTTGGTGGGG CCGCAGCCAT CCTGGGCCTG GTAGCCATGG CTTCTGATGT GGAAGGGCTG
    TATGCAGCGG TCAAGGCCCT AGTTTGTGTG GTCAAGAGTA ACCCACTAGC CAGCAAAGAA
    ATGGAAAGAA TCCGGGGCTA CCAGTTGCTG GCAATGCTGT TTAAGAAGAA ACGCTCCCTT
    CTTAACAGCC ACATTCTCCA TCTCACTTTT TCCTTGGTGG GAACTGTTGA CAGTGGACAT
    GAGACTTCCA TTATTCCAAA TTCAACAGCT TTCCAGGATC TACTTTGTGA TTTTGAAGTC
    TGGCTCCATG CTCCATATGA ACTTCATCTC TCCCTATTTG AACACTTTAT TGAATTGCTC
    ACAGAGTCCA GTGAAGCTTC AAAGAATGCC AAGTTGATGA GGGAATTCCA GCTAATCCCA
    AAGCTGCTAC TGACTCTTCG AGATATGTCT CTATCCCAGC CTACCATTGC TGCTATTAGT
    AACGTGCTGA GCTTCTTACT GCAGGGTTTT CCCAACAGCA ATGATCTACT CAGGTTTGGC
    CAGTTCATTT CTTCTACTTT ACCAACCTTT GCAGTTTGTG AGAAGTTTGT AGTTATGGAA
    ATAAACCACG AAGAGAAGCC TGACACTGGA ACTGAAGAAG AGTTTGGAGG TCTTGTATCT
    GCTAATCTTA TACTTTTGAG GAACAGACTT CTGGATATCT TACTAAAACT GGTTTACACA
    TCTAAAGAAA AGACAAGCAT TAATTTGCAA GCTTGTGAAG AGCTGGTCAG GACGCTGGGC
    TTTGACTGGA TTATGATGTT TATGGAAGAA CACTTGCATT CTACCACAGT CACAGCAGCC
    ATGAGGATTC TTGTTGTCCT ACTGAGTAAT CAGTCTATTC TCATCAAGTT CAAGGAGGGA
    CTCAGTGGTG GAGGCTGGCT GGAGCAGACA GATTCTGTCT TAACGAATAA GATCGGAACT
    GTATTAGGAT TCAATGTGGG CAGGAGTGCT GGCGGACGAT CAACTGTCAG GGAGATTAAC
    CGGGATGCTT GTCACTTTCC TGGCTTTCCG GTGCTTCAGT CATTCCTTCC TAAACACACT
    AATGTCCCTG CTCTCTATTT CCTCCTCATG GCATTGTTTC TGCAGCAGCC CGTTAGTGAG
    CTGCCTGAGA ACCTGCAGGT CAGTGTGCCT GTCGTCAGCA GCCGATGTAA GCAGGGTTGC
    CAGTTTGATT TAGATTCCAT CTGGACCTTT ATCTTTGGAG TTCCTGCCTC CAGTGGAACC
    GTGGTCTCAT CTATCCACAA TGTGTGCACA GAAGCTGCTT TTTTGTTACT GGGCATGCTC
    CGAAGCATGC TGAATTCACC TTGGCAATCA GAAGAAGAGG GGTCTTGGCT CCGGGAGTAT
    CCTGTGACCC TGATGCAGTT CTTCAGATAC TTGTATCACA ATGTCCCGGA CCTGGCCTCC
    ATGTGGATGA ACCCTGATTT CCTGTGTGCA CTTGCAGCCA CGGTCTTCCC CTTCAACATC
    CGCCCTTATT CAGAAATGGT GACTGATCTC GATGATGAAG TTGGATCTCC AGCTGAAGAA
    TTTAAAGCCT TTGCAGCAGA CACAGGGATG AACAGAAGCC AATCAGAGTA CTGCAACGTG
    GGCGTCAAGA CATACCTGAC TAATCACCCA GCTAAGAAAT TTGTTTTTGA CTTCATGCGG
    GTCTTAATAA TAGACAACCT CTGTCTTACA CCTGCCAGCA AGCAGACTCC ACTGATTGAT
    CTTTTGTTGG AGGCTTCTCC TGAGAGATCG ACAAGAACGC AGCAAAAAGA ATTTCAGACT
    TACATTTTGG ATAGTGTGAT GGATCATTTG CTTGCAGCTG ATGTTTTACT GGGAGAAGAT
    GCATCTCTTC CTATCACCAG TGGAGGAAGC TACCAGGTAT TGGTGAACAA TGTGTTTTAT
    TTCACACAAC GTGTGGTGGA CAAGCTTTGG CAAGGCATGT TCAACAAAGA ATCTAAACTT
    CTTATAGATT TTATAATTCA ACTAATTGCA CAGTCAAAGA GAAGATCCCA GGGGTTGTCA
    CTGGATGCAG TCTATCACTG CCTCAACAGG ACTATCCTGT ACCAGTTCTC CCGGGCGCAC
    AAAACCGTCC CGCAGCAAGT AGCCCTTCTT GACTCTCTCA GGGTCCTGAC TGTCAACAGG
    AACTTGATCC TGGGCCCCGG AAACCACGAC CAAGAATTCA TCAGCTGTCT GGCTCACTGC
    CTCATTAACC TGCATGTTGG GAGCAATGTG GATGGATTTG GACTGGAAGC AGAAGCCCGC
    ATGACTACAT GGCATATTAT GATCCCCTCG GACATTGAAC CAGATGGTGG TTACAGCCAA
    GATATTAGTG AAGGGCGTCA GCTTCTCATA AAAGCTGTCA ACAGAGTTTG GACGGAATTA
    ATTCATAGTA AGAAACAAGT CTTGGAGGAG CTTTTCAAAG TCACTCTACC TGTGAATGAA
    AGAGGCCACG TGGACATAGC TATAGCAAGG CCGCTCATCG AAGAAGCTAG TTTGAAGTGC
    TGGCAGAATC ATCTAGCCCA TGAAAAGAAG TGCATAAGTC GAGGAGAAGC TTTAATGCCC
    ACCACACAAT CCAAATTGTC CCGTGTCAGC AGTGGCTTTG GTCTTTCCAA GTTAACGGGA
    TCAAGAAGGA ATCGAAAGGA AAGCGGGCTT CATAAACACA GCCTTTCCAC CCAGGAGATT
    TCTCAGTGGA TGTTTACACA CATCGCTGTT GTTCGTGACT TGGTAGACAC ACAATATAAG
    GAATACCAGG AGCGTCAGCA AAATGCCCTG AAGTACGTGA CGGAAGAGTG GTGCCAGATC
    GAGTATGAGC TGCTGCGGGA GCGGGGACTC TGGGGCCCCC CCATTGGTTC CCATCTCGAC
    AAGTGGATGT TGGAGATGAC AGAGGGTCCC TGCAGAATGA GGAAAAAAAT GGTGCGAAAT
    GATATGTTTT ATAACCATTA CCCTTACGTG CCAGAAACGG AGCAGGAAAC CAACGTGGCG
    TCTGAGATCC CAAGTAAACA GCCTGAGACA CCTGATGATA TCATTCCTCA AAAGAAGCCT
    GCTCGATACA GAAGAGCCGT AAGTTATGAC AGTAAAGAAT ACTACATGCG ACTAGCCTCC
    GGCAACCCAG CCATTGTGCA GGATGCCATC GTAGAGAGCT CTGAAGGAGA AGCTGCTCAG
    CAAGAACCAG AACATGGTGA AGACACGATT GCTAAAGTCA AAGGACTGGT CAAGCCACCT
    CTCAAACGGT CTCGGTCAGC ACCGGACGGA GGCGATGAGG AGACACAGGA GCAGTTACAG
    GACCAGACAG CTGAGGGCAG CTCGATAGAA GAGGAGGAGA AAACAGACAA CGCCACCCTG
    CTGCGCCTGT TGGAGGAGGG CGAAAAGATC CAGCACATGT ACCGCTGTGC TCGAGTCCAG
    GGCCTTGATA CCAGTGAGGG GCTACTCCTC TTTGGTAAAG AGCATTTCTA TGTGATTGAT
    GGATTTACAA TGACAGCAAC CAGAGAAATA AGAGATATTG AAACCTTACC TCCAAATATG
    CATGAGCCGA TTATCCCTAG AGGAGCCAGG CAAGGCCCCA GTCAGCTCAA GAGAACGTGC
    AGTATCTTTG CATATGAAGA TATCAAGGAA GTGCATAAAC GGAGATATCT CCTGCAGCCT
    ATCGCTGTGG AAGTTTTCTC TGGAGATGGA CGAAACTACC TCCTTGCTTT TCAGAAAGGA
    ATCAGAAACA AAGTTTATCA AAGGTTTTTG GCTGTAGTGC CATCTCTCAC TGACAGTTCA
    GAGTCTGTGT CTGGGCAGCG GCCGAACACC AGCGTGGAGC AGGGATCTGG GTTGCTTAGC
    ACTCTGGTTG GAGAGAAGTC TGTGACTCAG AGATGGGAGA GAGGTGAAAT CAGCAACTTT
    CAGTATTTGA TGCACTTGAA CACTTTGGCT GGAAGATCGT ATAATGACCT CATGCAATAT
    CCTGTCTTCC CCTGGATCCT TGCAGATTAC GACTCGGAGG AGGTCGATCT TACTAATCCC
    AAGACATTTA GAAACCTGGC CAAGCCAATG GGAGCACAGA CAGATGAACG ATTAGCTCAG
    TATAAGAAGC GATATAAAGA CTGGGAAGAT CCTAATGGAG AAACTCCAGC CTACCACTAT
    GGGACCCACT ATTCATCAGC CATGATTGTG GCTTCATACC TTGTCAGAAT GGAGCCCTTC
    ACACAGATAT TCCTAAGGCT ACAGGGAGGC CACTTTGACC TGGCTGACCG GATGTTTCAC
    AGCGTGCGTG AGGCCTGGTA CTCAGCGTCA AAGCACAACA TGGCAGATGT AAAGGAACTC
    ATCCCAGAAT TTTTTTACTT ACCAGAGTTC CTGTTCAATT CCAACAACTT TGACCTTGGC
    TGTAAACAAA ACGGCACCAA ACTTGGAGAT GTTATCCTTC CACCCTGGGC AAAAGGGGAC
    CCACGAGAAT TCATCCGGGT GCACCGTGAG GCTCTGGAAT GTGACTACGT GAGTGCCCAT
    CTGCATGAGT GGATCGACTT AATCTTCGGC TATAAGCAGC AAGGTCCTGC CGCAGTAGAA
    GCTGTAAACG TCTTCCATCA CCTTTTCTAT GAGGGTCAAG TGGATATCTA CAACATAAAT
    GACCCCCTCA AGGAAACAGC CACCATAGGG TTCATTAATA ACTTTGGGCA GATCCCTAAA
    CAGTTATTTA AAAAACCTCA TCCACCCAAG CGAGTGAGGA GTCGACTCAA CGGAGACAAT
    GCGGGGGCCT CCGTCCCGCC AGGGTCTGCC AGTGACAAGA TCTTTTTTCA TCATCTGGAC
    AACCTGAGGC CTTCACTAAC ACCTGTAAAA GAACTCAAAG AGCCTGTGGG ACAAATCGTG
    TGTACAGACA AAGGCATTCT TGCGGTAGAA CAGAATAAGG TTCTTATCCC ACCAACCTGG
    AACAAGACTT TCGCCTGGGG CTACGCAGAC CTCAGCTGCA GGCTGGGGAC CTATGAGTCA
    GACAAGGCAG TGACTGTTTA TGAATGTTTG TCGGAGTGGG GCCAGATTCT CTGTGCGATC
    TGCCCCAACC CCAAGCTGGT CATCACGGGT GGAACAAGTA CGGTGGTGTG TGTTTGGGAG
    ATGAGTACCT CGAAAGAAAA GGCTAAGACT CTCACTCTCA AACAGGCCTT ACTTGGCCAC
    ACGGATACGG TTACCTGTGC CACCGCGTCG TTAGCCTATC ACATCATCGT CAGCGGGTCC
    CGAGACCGAA CCTGCATCAT CTGGGACCTG AACAAGCTGT CATTCCTAAC CCAGCTCCGA
    GGCCACCGGG CTCCCGTGTC TGCTCTGTGT ATCAATGAAT TAACCGGGGA CATCGTGTCC
    TGCGCCGGCA CGTACATCCA TGTGTGGAGC ATAAACGGGA ACCCCGTCGT GAGTGTGAAC
    ACGTTCACAG GCAGGAGCCA GCAGATCGTC TGTTGCTGTG TGTCGGAGAT GAACGAGTGG
    GACACGCAGA ACGTCATCGT CACGGGACAC TCGGATGGCG TGGTCCGGTT TTGGAGAATG
    GAATTTTTGC AAGTTCCTGA AACACCAGCC CCTGAGCCTG CTGAAGTCCT CGAAATGCAG
    GAAGACTGTC CAGAAGCACA AATCGGGCAA GAAGCCCAGG ACGAGGACAG TAGCGATTCC
    GAGGCCGACG AGCAGAGCGT CAGCCAGGAC CCCAAGGACA CGCCCAGCCA GCCCAGCAGC
    ACCAGCCACC GGCCCCGGGC AGCCTCCTGC AGAGCCACAG CCACCTGGTA TAACGACAGT
    GGCTCCGACG ACTCCAGGCG CTGGTCCGAT CAGCTCAGCC TCGATGAGAA AGACGGCTTC
    ATATTTGTGA ACTATTCAGA GGGCCAGAGC AGACCTCATC TTCAGACTCC CCTTAACCAC
    CCCCACCCCA ATCCCATCGA GGCGCGGAAT TACAGCCGAT TGAAGCCTGG GTACCGCTGG
    GAACGGCAGC TCGTGTTCAG GAGTAAGCTG ACCATGCACA CGGCCTTTGA CCGGAAGGAC
    AATGCTCACC CTGCCGAGAT CACTGCATTG GGCGTCTCCA AGGACCACAG CAGGATCCTC
    GTGGGCGACA GTCGGGGCCG GGTGTTCAGC TGGTCTGTGA GCGACCAGCC CGGCCGCTCT
    GCCGCTGACC ACTGGGTGAA GGACGAAGGA GGGGACAGCT GTTCAGGCTG CTCCGTGCGG
    TTCTCTCTCA CGGAAAGACG GCACCATTGC AGGAACTGTG GTCAGCTCTT CTGCCAGAAG
    TGCAGCCGAT TTCAGTCAGA AATCAAGCGC TTGAAAATCT CCTCCCCAGT ACGTGTCTGT
    CAGAACTGTT ACTACAACTT ACAGCATGAG CGGAGTTCAG AAGACGGGCC TCGGAATTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.