POLR1A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR1A
Entrez Gene ID	536316
Full Name	RNA polymerase I subunit A
General protein information	Preferred Names RNA polymerase I subunit A Names DNA-directed RNA polymerase I subunit RPA1 polymerase (RNA) I polypeptide A, 194kDa
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001192876.2	NP_001179805.1	DNA-directed RNA polymerase I subunit RPA1

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLR1A	NP_056240.2
Pan troglodytes (chimpanzee)	POLR1A	XP_001135380.2
Caenorhabditis elegans (roundworm)	Y48E1A.1	NP_001022450.1
Eremothecium gossypii	AGOS_ADR374C	NP_984470.2
Schizosaccharomyces pombe (fission yeast)	nuc1	NP_596300.1
Saccharomyces cerevisiae (baker's yeast)	RPA190	NP_014986.3
Magnaporthe oryzae (rice blast fungus)	MGG_01158	XP_003717763.1
	POLR1A	XP_002808090.1
Gallus gallus (chicken)	POLR1A	XP_420851.3
Danio rerio (zebrafish)	polr1a	XP_001922839.2
Drosophila melanogaster (fruit fly)	RpI1	NP_523743.1
Xenopus tropicalis (tropical clawed frog)	polr1a	XP_002937328.2
Bos taurus (cattle)	POLR1A	NP_001179805.1
Rattus norvegicus (Norway rat)	Polr1a	NP_113960.1
Neurospora crassa	NCU01638	XP_956006.2
Arabidopsis thaliana (thale cress)	NRPA1	NP_191325.1
Canis lupus familiaris (dog)	POLR1A	XP_532976.3
Mus musculus (house mouse)	Polr1a	NP_033114.3
Kluyveromyces lactis	KLLA0F23243g	XP_456115.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLR1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa39503	NM_001192876.2 Latest version!	Bos taurus RNA polymerase I subunit A (POLR1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR1A ( NM_001192876.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179805.1 Bos taurus RNA polymerase I subunit A (POLR1A), mRNA.

Price & Availability↑

CloneID	OBa39503
Clone ID Related Accession (Same CDS sequence)	NM_001192876.2
Accession Version	NM_001192876.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	DNA-directed RNA polymerase I subunit RPA1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000011.1. On Feb 23, 2016 this sequence version replaced NM_001192876.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.47349.1, SRR6764281.40738.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGTTGGGCT CAAAGAACAT GCCCTGGCGG CGGCTGCAGG GCATTTCCTT CGGGATGTAT 
TCGGCCGAAG AACTCAAGAA ATTAAGCGTG AAGTCCATTA CGAACCCTCG GTACCTGGAC 
AGCCTGGGGA ACCCCTCGGT AAATGGCCTG TATGATTTAG CTCTGGGCCC CGCTGACTCC 
AAAGAGGTGT GCTCCACCTG CGTGCAGGAC TTCACCAACT GCTCTGGGCA CTTGGGCCAC 
ATCGACCTCC CGCTCACCGT GTACAACCCT CTCCTCTTCG ATAAACTCTA CCTTTTGCTC 
CGGGGCTCCT GTTTAAACTG TCACATGCTG ACCTGCCCCC GGGCTGTGGT TCACCTCTTA 
CTGTGCCAGC TGAGAGTCCT GGAAGTTGGG GCCCTGCAAG CAGTGTATGA GCTGGAGAGA 
ATTCTCTACA GGTTTCTGGA AGAAAATGCT GATCCTACTG CCTGTGAAAT TCAGGAAGAG 
CTAGAGCAAT ATACAATCAA AATCCTGCAG AACAACCTCC CAGGATCCCA GGGTGCACAA 
GTGAAAAATG TCTGTGAGAG CAAGAGCAAA CTCATCTCTG TCTTCTGGAA GGTACATATG 
ACTGCTAAGA GATGTCCCCA CTGCAAAACT GGGCGGTCAG TGGTCCGGAA GGAGCACAAT 
AGCAAACTGA CCATCACGTA CCCGGCCATG GTGCACAGGA GAGCTGACCA GAAGGACAGT 
GAGCCCCAGG GAATTGAGGA AGCTCAGATG GGAAAACGAG GATACCTGAC ACCCTCCAGT 
GCGCAAGAAC ATCTCATAGC TCTTTGGAAA AATGAAGGGT TCTTTCTGAA TTACCTTTTT 
TTGGGACTGG AAGATGTTGG CATGGAGTCC AGGTTCAACC CCAGTATGTT CTTTCTGGAT 
TTCTTGGTGG TACCACCCTC AAGGTATCGC CCTGTCAACC GCCTTGGGGA CCAGATGTTC 
ACCAACGGGC AGACGGTGAA CTTGCAGGCT GTCATGAAGG ACGTGGTTCT GATCCGAAAG 
CTTCTGGCAC TGATGGCCCA AGAACAGGAG CTGCCAAGTG AGGTGGCAGC ACCCACTACA 
GAAGAGGAAA AAGACTCCTG GATGGCTATG GACCGATCTT TTTTGAGTCA GCTTCCAGGC 
CAGTCCCTCA CAGACAGACT TTACAACATT TGGATTCGCC TTCAGAGCCA CGTTAATATT 
GTGTTTGATA GCGAGATGGA CAAATTAATG ATGGAAAAGT ACCCCGGCAT TAGACAGATC 
CTGGAGAAGA AGGATGGTTT GTTCCGAAAG CACATGATGG GGAAGCGCGT AGACTACGCA 
GCCCGCTCGG TCATCTGCCC GGACATGTAC ATCAGTACCA ACGAAATTGG AATTCCCATG 
GTGTTTGCCA CAAAGCTGAC CTACCCTCAA CCAGTCACCC CCTGGAACGT TCAGGAACTG 
AGGCAGGCGG TCATCAACGG CCCCAACATG CACCCTGGAG CCTCTATGGT CATCAACGAG 
GATGGCAGTC GCACAGCCCT GAGCGCCTCG GACCTCACGC AGCGGGAGGC CGTGGCCAAA 
CAGCTCCTGA CACCAGCTAC GGGGGCCCCT AAGCCCCAGG GGACAAAAAT TGTGTGCCGG 
CATGTGAAGA ACGGGGACAT TCTCCTCCTG AACCGGCAGC CCACCCTGCA CAGGCCCTCC 
ATCCAGGCGC ATCGCGCCCG CGTCCTCCCC GAGGAGAAAG TCCTGCGGCT CCACTATGCC 
AACTGCAAGG CCTACAACGC CGACTTCGAT GGAGATGAGA TGAACGCCCA CTTTCCCCAG 
AGTGAACTGG GCCGGGCCGA GGCCTACGTC CTGGCCTGCA CCGATCAGCA GTACCTTGTT 
CCCAAGGATG GCCAGCCATT GGCGGGGCTG ATCCAAGATC ACATGGTTTC AGGAGCAAAC 
ATGACCATTC GAGGCTGCTT TTTCAACCGG GAGCAGTATA TGGAGCTGGT GTTCCGAGGA 
CTCACAGACA AAGTGGGGCG TGTGAAGCTC TTTCCTCCTG CCATCCTGAA GCCCTTTCCT 
CTGTGGACAG GAAAGCAGGT CGTGTCCACA TTGCTCATCA ACATAATCCC AGAGGACCAT 
ATCCCCCTGA ACTTGTCAGG AAAGGCGAAG ATCGGTGGAA AGGCCTGGGT GAAGGAGTCT 
CCTCGGCTCG TTCCCGGCTT TAACCCCGAC TCCATGTGCG AGTCCCAGGT GATCATCAGA 
GCAGGGGAAC TGCTGTGCGG GGTGCTGGAC AAGGCGCACT ACGGAAGCTC CGCCTACGGC 
CTGGTCCACT GCTGCTACGA GATCTACGGT GGGGAGACCA GCGGCAGGGT TCTCACCTGC 
CTGGCCCGCC TCTTCACCGC CTACCTGCAG CTCTACAGAG GCTTCACCTT GGGAGTAGAA 
GACATTTTGG TCAAGCCGAA GGCGGATGTC AGGAGACATG GGATCATTGA AGACTCCACC 
CACTGTGGGC CCCGGGCCGT CAGGGCTGCG TTAAACCTGC CAGAGGCAGT GTCATGTGAT 
GAAGTCCAAG GGAAGTGGCA GGATGCCCAT CTGAGTAAAG ATCAGAGGGA TTTTAACATG 
ATTGACTTGA AGTTCAAGGA GGAAGTGAAC CATTACAGCA ATGAAATTAA CAAGGCATGC 
ATGCCCTTCG GCCTACACAG ACAGTTCCCA GAGAACAATC TGCAGATGAT GGTACAGTCT 
GGAGCCAAAG GTTCAACAGT GAACACCATG CAGATTTCGT GCCTGCTGGG CCAGATCGAA 
TTGGAAGGTC GGAGACCCCC GCTCATGGCA TCTGGCAAGT CGTTGCCCTG CTTCGAGCCT 
TATGAATTCA CCCCCAGGGC TGGCGGCTTC GTGACTGGCA GGTTCCTCAC TGGCATCAAG 
CCTCCGGAGT TCTTCTTCCA CTGCATGGCC GGCCGAGAGG GTCTGGTGGA CACTGCTGTG 
AAGACCAGCC GTTCCGGATA CCTCCAGAGG TGCATCATCA AACACCTGGA GGGGCTGGTT 
GTCCAATACG ACCTGACAGT CCGCGACAGT GATGGCAGCG TGGTGCAGTT CCTGTACGGG 
GAGGACGGCC TGGACATCCC CAAGACACAG TTCCTGCAGC CTAAGCAGTT CCCCTTCCTC 
ACCAGCAACT ATGAGGTAAT AATGAAATCA AAGCATCTCC ATGAAGTTTT ATCCAGAGCG 
GATCCCCAGA AAGCTCTCCG TCATTTCAGA GCCATCAAAA AATGGCACAG CAAGCATTCC 
AACACCCTCC TGAGGAAAGG CGCCTTCTTG AATTACTCCC AGAAAATTCA GGCAGCTGTG 
AAAGCCTTGA ACCTTGAGGG CACAAACCAG AACGGCCGCA GCCCCGAGAC TTACCAGATG 
CTGAGGATGT GGGCTGAGTT AGACGAACAA AGCCGGAGGA AGTACCAGAA GAAGGCGGCT 
CCTTGTCCCG ACCCCAGTCT GTCTGTCTGG CGCCCAGACA TCTACTTCGC ATCCGTGTCA 
GAAACATTTG AAAAGAAGAT GGACGACTAC AGCTGCGAGT GGGCAGCTCA GGCAGAGCGG 
AGTTACGAGA AATCGGGGCT TTCTCTCGAC AGTTTGAGGA CTCTGCTGCA GCTCAAGTGG 
CAGCGCTCTC TATGCGACCC GGGTGAGGCC GTGGGCCTGC TGGCCGCCCA GAGCATCGGG 
GAGCCCTCCA CACAGATGAC GCTCAACACC TTCCACTTTG CCGGCAGAGG CGAGATGAAT 
GTCACCCTGG GCATTCCGAG GTTGCGGGAG ATCCTCATGG TGGCCAGTTC CAACATCAAG 
ACGCCGATGA TGAGTGTACC TGTGTTCAAT ACCAAGAAAG CACTGAAGAA AGTGAAAAGC 
CTGAAGAAGC AACTCACCAG AGTGTGTCTC GGGGAGGTGT TGGAGAAAAT TGATGTCCAG 
GAGTCCTTCC GTATAGGGGA GAAACAGAAC AAGTTCCGGG TATACCAGCT GCGGTTTCAG 
TTCCTGCCGC ATGCGTACTA CCAGCAGGAG AAGTGCACGA GGCCCGAGGA CATCTTACAC 
TTCATGGAAA CCAGATTCTT TAAAATTCTG ATGGAAGCCA TCAGGAAGAA GAACAGCAAA 
GCATCAGCTT TCAGAAATGT GAACACGCGG AAAGCCACCC AGCGGGATCT AGACAATGCT 
GGCGAGTCCG AGAGGAATCG GGGAGAGCAG GAGGGCGACG AGGAAGAGGA GGGGCACATT 
GTGGACGCTG AAGCTGAGGA GGGAGATGCT GATGCCTCAG ATGCCAAACG CAAGGAAAAA 
CAAGAGGAGG AGGTCGACTA TGACAGCGAG GAGGAGGAGG AGAAGGAGGA AGAGGAGAAG 
GAGGAAGAGG ACATTCAGGA GGAAGGGACC GGCCTCAAAG AAGGCGTTCA GCAGGAGCAG 
GAGGAAGGCG GCTGGGCCCC CGAAGAGAGC TCGCTCCCCG GTGCCCCCCT GACGCAGCCA 
CAGAAGCGCG CCCGAAGCCG GGAGCCCCAG GGGGCCGAGG CGGTGGAGCG CCGGGCACAG 
GCGGTGCGCG AGTGCCATCC GTTCATAGAG GACTACCAGT ATGACACACA GGGCAGCCTG 
TGGTGCCAGG TGACGCTGAA GCTCCCTCTC ATGAAAGTCA ACTTTGACAT GAGCTCCCTG 
GTAGCGTCCT TGGCCCAAGG AGCCATCATC TATGCAACCA AGGGTATCAC AAGGTGCCTC 
CTGAATGAGA CCATCAACAG TAAGAATGAG AAGGAGCTTG TCTTAAACAC AGAAGGCATC 
AACCTCCCGG AGCTGTTCAA GTACGCCGAG GTCCTAGATC TCCGCCGTCT CTATTCCAAT 
GACATCCATG CCATGGCCAG CACGTACGGC ATCGAGGCCG CACTGCGAGT GATTGAAAAG 
GAGATCAAGG ACGTGTTTGC CGTATACGGC ATCGCCGTTG ACCCCCGCCA CCTCTCGCTG 
GTGGCTGACT ACATGTGCTT CGAGGGTGTT TACAAGCCAC TGAACCGCTT TGGGATCCGG 
TCAAACTCCT CGCCACTGCA GCAGATGACG TTTGAGACCA GCTTCCAGTT TCTGAAGCAG 
GCCACCCTCA TGGGATCCCA TGACGAACTG AGGTCCCCAT CAGCCTGCCT CGTGGTCGGG 
AAGGTCGTCA AGGGTGGGAC CGGCCTCTTC GAGCTCAAGC AGCCACTAAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179805.1
CDS	1..5154
Translation	MLGSKNMPWRRLQGISFGMYSAEELKKLSVKSITNPRYLDSLGNPSVNGLYDLALGPADSKEVCSTCVQDFTNCSGHLGHIDLPLTVYNPLLFDKLYLLLRGSCLNCHMLTCPRAVVHLLLCQLRVLEVGALQAVYELERILYRFLEENADPTACEIQEELEQYTIKILQNNLPGSQGAQVKNVCESKSKLISVFWKVHMTAKRCPHCKTGRSVVRKEHNSKLTITYPAMVHRRADQKDSEPQGIEEAQMGKRGYLTPSSAQEHLIALWKNEGFFLNYLFLGLEDVGMESRFNPSMFFLDFLVVPPSRYRPVNRLGDQMFTNGQTVNLQAVMKDVVLIRKLLALMAQEQELPSEVAAPTTEEEKDSWMAMDRSFLSQLPGQSLTDRLYNIWIRLQSHVNIVFDSEMDKLMMEKYPGIRQILEKKDGLFRKHMMGKRVDYAARSVICPDMYISTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNMHPGASMVINEDGSRTALSASDLTQREAVAKQLLTPATGAPKPQGTKIVCRHVKNGDILLLNRQPTLHRPSIQAHRARVLPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGANMTIRGCFFNREQYMELVFRGLTDKVGRVKLFPPAILKPFPLWTGKQVVSTLLINIIPEDHIPLNLSGKAKIGGKAWVKESPRLVPGFNPDSMCESQVIIRAGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGRVLTCLARLFTAYLQLYRGFTLGVEDILVKPKADVRRHGIIEDSTHCGPRAVRAALNLPEAVSCDEVQGKWQDAHLSKDQRDFNMIDLKFKEEVNHYSNEINKACMPFGLHRQFPENNLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLTSNYEVIMKSKHLHEVLSRADPQKALRHFRAIKKWHSKHSNTLLRKGAFLNYSQKIQAAVKALNLEGTNQNGRSPETYQMLRMWAELDEQSRRKYQKKAAPCPDPSLSVWRPDIYFASVSETFEKKMDDYSCEWAAQAERSYEKSGLSLDSLRTLLQLKWQRSLCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASSNIKTPMMSVPVFNTKKALKKVKSLKKQLTRVCLGEVLEKIDVQESFRIGEKQNKFRVYQLRFQFLPHAYYQQEKCTRPEDILHFMETRFFKILMEAIRKKNSKASAFRNVNTRKATQRDLDNAGESERNRGEQEGDEEEEGHIVDAEAEEGDADASDAKRKEKQEEEVDYDSEEEEEKEEEEKEEEDIQEEGTGLKEGVQQEQEEGGWAPEESSLPGAPLTQPQKRARSREPQGAEAVERRAQAVRECHPFIEDYQYDTQGSLWCQVTLKLPLMKVNFDMSSLVASLAQGAIIYATKGITRCLLNETINSKNEKELVLNTEGINLPELFKYAEVLDLRRLYSNDIHAMASTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIRSNSSPLQQMTFETSFQFLKQATLMGSHDELRSPSACLVVGKVVKGGTGLFELKQPLR

Target ORF information:

RefSeq Version	NM_001192876.2
Organism	Bos taurus(cattle)
Definition	Bos taurus RNA polymerase I subunit A (POLR1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192876.2

ORF Insert Sequence:

ATGTTGGGCT CAAAGAACAT GCCCTGGCGG CGGCTGCAGG GCATTTCCTT CGGGATGTAT 
TCGGCCGAAG AACTCAAGAA ATTAAGCGTG AAGTCCATTA CGAACCCTCG GTACCTGGAC 
AGCCTGGGGA ACCCCTCGGT AAATGGCCTG TATGATTTAG CTCTGGGCCC CGCTGACTCC 
AAAGAGGTGT GCTCCACCTG CGTGCAGGAC TTCACCAACT GCTCTGGGCA CTTGGGCCAC 
ATCGACCTCC CGCTCACCGT GTACAACCCT CTCCTCTTCG ATAAACTCTA CCTTTTGCTC 
CGGGGCTCCT GTTTAAACTG TCACATGCTG ACCTGCCCCC GGGCTGTGGT TCACCTCTTA 
CTGTGCCAGC TGAGAGTCCT GGAAGTTGGG GCCCTGCAAG CAGTGTATGA GCTGGAGAGA 
ATTCTCTACA GGTTTCTGGA AGAAAATGCT GATCCTACTG CCTGTGAAAT TCAGGAAGAG 
CTAGAGCAAT ATACAATCAA AATCCTGCAG AACAACCTCC CAGGATCCCA GGGTGCACAA 
GTGAAAAATG TCTGTGAGAG CAAGAGCAAA CTCATCTCTG TCTTCTGGAA GGTACATATG 
ACTGCTAAGA GATGTCCCCA CTGCAAAACT GGGCGGTCAG TGGTCCGGAA GGAGCACAAT 
AGCAAACTGA CCATCACGTA CCCGGCCATG GTGCACAGGA GAGCTGACCA GAAGGACAGT 
GAGCCCCAGG GAATTGAGGA AGCTCAGATG GGAAAACGAG GATACCTGAC ACCCTCCAGT 
GCGCAAGAAC ATCTCATAGC TCTTTGGAAA AATGAAGGGT TCTTTCTGAA TTACCTTTTT 
TTGGGACTGG AAGATGTTGG CATGGAGTCC AGGTTCAACC CCAGTATGTT CTTTCTGGAT 
TTCTTGGTGG TACCACCCTC AAGGTATCGC CCTGTCAACC GCCTTGGGGA CCAGATGTTC 
ACCAACGGGC AGACGGTGAA CTTGCAGGCT GTCATGAAGG ACGTGGTTCT GATCCGAAAG 
CTTCTGGCAC TGATGGCCCA AGAACAGGAG CTGCCAAGTG AGGTGGCAGC ACCCACTACA 
GAAGAGGAAA AAGACTCCTG GATGGCTATG GACCGATCTT TTTTGAGTCA GCTTCCAGGC 
CAGTCCCTCA CAGACAGACT TTACAACATT TGGATTCGCC TTCAGAGCCA CGTTAATATT 
GTGTTTGATA GCGAGATGGA CAAATTAATG ATGGAAAAGT ACCCCGGCAT TAGACAGATC 
CTGGAGAAGA AGGATGGTTT GTTCCGAAAG CACATGATGG GGAAGCGCGT AGACTACGCA 
GCCCGCTCGG TCATCTGCCC GGACATGTAC ATCAGTACCA ACGAAATTGG AATTCCCATG 
GTGTTTGCCA CAAAGCTGAC CTACCCTCAA CCAGTCACCC CCTGGAACGT TCAGGAACTG 
AGGCAGGCGG TCATCAACGG CCCCAACATG CACCCTGGAG CCTCTATGGT CATCAACGAG 
GATGGCAGTC GCACAGCCCT GAGCGCCTCG GACCTCACGC AGCGGGAGGC CGTGGCCAAA 
CAGCTCCTGA CACCAGCTAC GGGGGCCCCT AAGCCCCAGG GGACAAAAAT TGTGTGCCGG 
CATGTGAAGA ACGGGGACAT TCTCCTCCTG AACCGGCAGC CCACCCTGCA CAGGCCCTCC 
ATCCAGGCGC ATCGCGCCCG CGTCCTCCCC GAGGAGAAAG TCCTGCGGCT CCACTATGCC 
AACTGCAAGG CCTACAACGC CGACTTCGAT GGAGATGAGA TGAACGCCCA CTTTCCCCAG 
AGTGAACTGG GCCGGGCCGA GGCCTACGTC CTGGCCTGCA CCGATCAGCA GTACCTTGTT 
CCCAAGGATG GCCAGCCATT GGCGGGGCTG ATCCAAGATC ACATGGTTTC AGGAGCAAAC 
ATGACCATTC GAGGCTGCTT TTTCAACCGG GAGCAGTATA TGGAGCTGGT GTTCCGAGGA 
CTCACAGACA AAGTGGGGCG TGTGAAGCTC TTTCCTCCTG CCATCCTGAA GCCCTTTCCT 
CTGTGGACAG GAAAGCAGGT CGTGTCCACA TTGCTCATCA ACATAATCCC AGAGGACCAT 
ATCCCCCTGA ACTTGTCAGG AAAGGCGAAG ATCGGTGGAA AGGCCTGGGT GAAGGAGTCT 
CCTCGGCTCG TTCCCGGCTT TAACCCCGAC TCCATGTGCG AGTCCCAGGT GATCATCAGA 
GCAGGGGAAC TGCTGTGCGG GGTGCTGGAC AAGGCGCACT ACGGAAGCTC CGCCTACGGC 
CTGGTCCACT GCTGCTACGA GATCTACGGT GGGGAGACCA GCGGCAGGGT TCTCACCTGC 
CTGGCCCGCC TCTTCACCGC CTACCTGCAG CTCTACAGAG GCTTCACCTT GGGAGTAGAA 
GACATTTTGG TCAAGCCGAA GGCGGATGTC AGGAGACATG GGATCATTGA AGACTCCACC 
CACTGTGGGC CCCGGGCCGT CAGGGCTGCG TTAAACCTGC CAGAGGCAGT GTCATGTGAT 
GAAGTCCAAG GGAAGTGGCA GGATGCCCAT CTGAGTAAAG ATCAGAGGGA TTTTAACATG 
ATTGACTTGA AGTTCAAGGA GGAAGTGAAC CATTACAGCA ATGAAATTAA CAAGGCATGC 
ATGCCCTTCG GCCTACACAG ACAGTTCCCA GAGAACAATC TGCAGATGAT GGTACAGTCT 
GGAGCCAAAG GTTCAACAGT GAACACCATG CAGATTTCGT GCCTGCTGGG CCAGATCGAA 
TTGGAAGGTC GGAGACCCCC GCTCATGGCA TCTGGCAAGT CGTTGCCCTG CTTCGAGCCT 
TATGAATTCA CCCCCAGGGC TGGCGGCTTC GTGACTGGCA GGTTCCTCAC TGGCATCAAG 
CCTCCGGAGT TCTTCTTCCA CTGCATGGCC GGCCGAGAGG GTCTGGTGGA CACTGCTGTG 
AAGACCAGCC GTTCCGGATA CCTCCAGAGG TGCATCATCA AACACCTGGA GGGGCTGGTT 
GTCCAATACG ACCTGACAGT CCGCGACAGT GATGGCAGCG TGGTGCAGTT CCTGTACGGG 
GAGGACGGCC TGGACATCCC CAAGACACAG TTCCTGCAGC CTAAGCAGTT CCCCTTCCTC 
ACCAGCAACT ATGAGGTAAT AATGAAATCA AAGCATCTCC ATGAAGTTTT ATCCAGAGCG 
GATCCCCAGA AAGCTCTCCG TCATTTCAGA GCCATCAAAA AATGGCACAG CAAGCATTCC 
AACACCCTCC TGAGGAAAGG CGCCTTCTTG AATTACTCCC AGAAAATTCA GGCAGCTGTG 
AAAGCCTTGA ACCTTGAGGG CACAAACCAG AACGGCCGCA GCCCCGAGAC TTACCAGATG 
CTGAGGATGT GGGCTGAGTT AGACGAACAA AGCCGGAGGA AGTACCAGAA GAAGGCGGCT 
CCTTGTCCCG ACCCCAGTCT GTCTGTCTGG CGCCCAGACA TCTACTTCGC ATCCGTGTCA 
GAAACATTTG AAAAGAAGAT GGACGACTAC AGCTGCGAGT GGGCAGCTCA GGCAGAGCGG 
AGTTACGAGA AATCGGGGCT TTCTCTCGAC AGTTTGAGGA CTCTGCTGCA GCTCAAGTGG 
CAGCGCTCTC TATGCGACCC GGGTGAGGCC GTGGGCCTGC TGGCCGCCCA GAGCATCGGG 
GAGCCCTCCA CACAGATGAC GCTCAACACC TTCCACTTTG CCGGCAGAGG CGAGATGAAT 
GTCACCCTGG GCATTCCGAG GTTGCGGGAG ATCCTCATGG TGGCCAGTTC CAACATCAAG 
ACGCCGATGA TGAGTGTACC TGTGTTCAAT ACCAAGAAAG CACTGAAGAA AGTGAAAAGC 
CTGAAGAAGC AACTCACCAG AGTGTGTCTC GGGGAGGTGT TGGAGAAAAT TGATGTCCAG 
GAGTCCTTCC GTATAGGGGA GAAACAGAAC AAGTTCCGGG TATACCAGCT GCGGTTTCAG 
TTCCTGCCGC ATGCGTACTA CCAGCAGGAG AAGTGCACGA GGCCCGAGGA CATCTTACAC 
TTCATGGAAA CCAGATTCTT TAAAATTCTG ATGGAAGCCA TCAGGAAGAA GAACAGCAAA 
GCATCAGCTT TCAGAAATGT GAACACGCGG AAAGCCACCC AGCGGGATCT AGACAATGCT 
GGCGAGTCCG AGAGGAATCG GGGAGAGCAG GAGGGCGACG AGGAAGAGGA GGGGCACATT 
GTGGACGCTG AAGCTGAGGA GGGAGATGCT GATGCCTCAG ATGCCAAACG CAAGGAAAAA 
CAAGAGGAGG AGGTCGACTA TGACAGCGAG GAGGAGGAGG AGAAGGAGGA AGAGGAGAAG 
GAGGAAGAGG ACATTCAGGA GGAAGGGACC GGCCTCAAAG AAGGCGTTCA GCAGGAGCAG 
GAGGAAGGCG GCTGGGCCCC CGAAGAGAGC TCGCTCCCCG GTGCCCCCCT GACGCAGCCA 
CAGAAGCGCG CCCGAAGCCG GGAGCCCCAG GGGGCCGAGG CGGTGGAGCG CCGGGCACAG 
GCGGTGCGCG AGTGCCATCC GTTCATAGAG GACTACCAGT ATGACACACA GGGCAGCCTG 
TGGTGCCAGG TGACGCTGAA GCTCCCTCTC ATGAAAGTCA ACTTTGACAT GAGCTCCCTG 
GTAGCGTCCT TGGCCCAAGG AGCCATCATC TATGCAACCA AGGGTATCAC AAGGTGCCTC 
CTGAATGAGA CCATCAACAG TAAGAATGAG AAGGAGCTTG TCTTAAACAC AGAAGGCATC 
AACCTCCCGG AGCTGTTCAA GTACGCCGAG GTCCTAGATC TCCGCCGTCT CTATTCCAAT 
GACATCCATG CCATGGCCAG CACGTACGGC ATCGAGGCCG CACTGCGAGT GATTGAAAAG 
GAGATCAAGG ACGTGTTTGC CGTATACGGC ATCGCCGTTG ACCCCCGCCA CCTCTCGCTG 
GTGGCTGACT ACATGTGCTT CGAGGGTGTT TACAAGCCAC TGAACCGCTT TGGGATCCGG 
TCAAACTCCT CGCCACTGCA GCAGATGACG TTTGAGACCA GCTTCCAGTT TCTGAAGCAG 
GCCACCCTCA TGGGATCCCA TGACGAACTG AGGTCCCCAT CAGCCTGCCT CGTGGTCGGG 
AAGGTCGTCA AGGGTGGGAC CGGCCTCTTC GAGCTCAAGC AGCCACTAAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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POLR1A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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