KIDINS220 cDNA ORF clone, Bos taurus(cattle)

The following KIDINS220 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIDINS220 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa220914 XM_024999552.1
Latest version!
Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa220915 XM_024999553.1
Latest version!
Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa220916 XM_024999554.1
Latest version!
Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa53366 XM_015465446.1
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa53366 XM_015465445.1
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa53366 XM_010810308.2
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa53366 XM_010810309.2
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa220911 XM_024999549.1
Latest version!
Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa220912 XM_024999550.1
Latest version!
Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa220913 XM_024999551.1
Latest version!
Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24271 XM_015465440.1
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24271 XM_005213101.4
Latest version!
Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24271 XM_024999548.1
Latest version!
Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24273 XM_015465441.1
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24273 XM_005213103.4
Latest version!
Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24274 XM_003586654.5
Latest version!
Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24274 XM_003582816.4
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24275 XM_015465443.1
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24275 XM_005213104.4
Latest version!
Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24276 XM_015465444.1
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24276 XM_005213105.4
Latest version!
Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa53363 XM_015473572.1
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa53363 XM_010810305.2
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa53363 XM_015465438.1
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa53363 XM_015465437.1
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa53364 XM_010810306.2
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa53364 XM_015465439.1
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa53365 XM_015465442.1
Latest version!
Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa53365 XM_010810307.3
Latest version!
Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
View Old Accession Versions >>
OBa24271 XM_005213101.3 Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24274 XM_003586654.4 Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa53365 XM_010810307.2 Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24276 XM_005213105.3 Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24275 XM_005213104.3 Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa24273 XM_005213103.3 Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa220914
    Clone ID Related Accession (Same CDS sequence) XM_024999552.1
    Accession Version XM_024999552.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GCCGTGTGAC
    TCTGGGTTTA ACAAACAGAG ACAGGGATCA GGCCCAGCCT CAGGCACAAT ACCACTGAAT
    TCGATGAACG TGGATGCAGT TTGTGAGAAA CTGAAACAAA TTGAAGGGCT GGACCAGAGC
    ATGCTGCCTC AGTACTGTGC AACCATCAAA AAGGCGAACA TAAATGGCCG TGTGTTGGCT
    CAGTGTAACA TTGATGAACT GAAGAAGGAA ATGAATATGA ATTTTGGAGA CTGGCATCTT
    TTTAGAAGCA CAATATTAGA AATGAGAAAT GCAGAAAACC AAGTGGTCCC TGAAGACCCA
    CGTTTACTGA GTGAAAACAG CAGCTCTGTT CCTCACGGTG AACCTGCTCG TCGCTCCGCA
    CACAGCGAGC TGCCCCACAC CGAGCTCTCC AGTCAGGCTC CCTACACCCT GAACTTCAGC
    TTTGAAGAGC TGAACACGCT CGGCCTGGAC GAAGGGGCTC CTCGCCACAG CAACCTGAGT
    TGGCAGAAAC TTCATGTTGT GCAAGTTGAA AAAGGTTCTT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855320.1
    CDS214..4338
    Translation

    Target ORF information:

    RefSeq Version XM_024999552.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999552.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GCCGTGTGAC
    TCTGGGTTTA ACAAACAGAG ACAGGGATCA GGCCCAGCCT CAGGCACAAT ACCACTGAAT
    TCGATGAACG TGGATGCAGT TTGTGAGAAA CTGAAACAAA TTGAAGGGCT GGACCAGAGC
    ATGCTGCCTC AGTACTGTGC AACCATCAAA AAGGCGAACA TAAATGGCCG TGTGTTGGCT
    CAGTGTAACA TTGATGAACT GAAGAAGGAA ATGAATATGA ATTTTGGAGA CTGGCATCTT
    TTTAGAAGCA CAATATTAGA AATGAGAAAT GCAGAAAACC AAGTGGTCCC TGAAGACCCA
    CGTTTACTGA GTGAAAACAG CAGCTCTGTT CCTCACGGTG AACCTGCTCG TCGCTCCGCA
    CACAGCGAGC TGCCCCACAC CGAGCTCTCC AGTCAGGCTC CCTACACCCT GAACTTCAGC
    TTTGAAGAGC TGAACACGCT CGGCCTGGAC GAAGGGGCTC CTCGCCACAG CAACCTGAGT
    TGGCAGAAAC TTCATGTTGT GCAAGTTGAA AAAGGTTCTT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220915
    Clone ID Related Accession (Same CDS sequence) XM_024999553.1
    Accession Version XM_024999553.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGGATCA
    GGCCCAGCCT CAGGCACAAT ACCACTGAAT TCGATGAACG TGGATGCAGT TTGTGAGAAA
    CTGAAACAAA TTGAAGGGCT GGACCAGAGC ATGCTGCCTC AGTACTGTGC AACCATCAAA
    AAGGCGAACA TAAATGGCCG TGTGTTGGCT CAGTGTAACA TTGATGAACT GAAGAAGGAA
    ATGAATATGA ATTTTGGAGA CTGGCATCTT TTTAGAAGCA CAATATTAGA AATGAGAAAT
    GCAGAAAACC AAGTGGTCCC TGAAGACCCA CGTTTACTGA GTGAAAACAG CAGCTCTGTT
    CCTCACGGTG AACCTGCTCG TCGCTCCGCA CACAGCGAGC TGCCCCACAC CGAGCTCTCC
    AGTCAGGCTC CCTACACCCT GAACTTCAGC TTTGAAGAGC TGAACACGCT CGGCCTGGAC
    GAAGGGGCTC CTCGCCACAG CAACCTGAGT TGGCAGAAAC TTCATGTTGT GCAAGTTGAA
    AAAGGTTCTT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855321.1
    CDS213..4247
    Translation

    Target ORF information:

    RefSeq Version XM_024999553.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999553.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGGATCA
    GGCCCAGCCT CAGGCACAAT ACCACTGAAT TCGATGAACG TGGATGCAGT TTGTGAGAAA
    CTGAAACAAA TTGAAGGGCT GGACCAGAGC ATGCTGCCTC AGTACTGTGC AACCATCAAA
    AAGGCGAACA TAAATGGCCG TGTGTTGGCT CAGTGTAACA TTGATGAACT GAAGAAGGAA
    ATGAATATGA ATTTTGGAGA CTGGCATCTT TTTAGAAGCA CAATATTAGA AATGAGAAAT
    GCAGAAAACC AAGTGGTCCC TGAAGACCCA CGTTTACTGA GTGAAAACAG CAGCTCTGTT
    CCTCACGGTG AACCTGCTCG TCGCTCCGCA CACAGCGAGC TGCCCCACAC CGAGCTCTCC
    AGTCAGGCTC CCTACACCCT GAACTTCAGC TTTGAAGAGC TGAACACGCT CGGCCTGGAC
    GAAGGGGCTC CTCGCCACAG CAACCTGAGT TGGCAGAAAC TTCATGTTGT GCAAGTTGAA
    AAAGGTTCTT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220916
    Clone ID Related Accession (Same CDS sequence) XM_024999554.1
    Accession Version XM_024999554.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGGATCAGGC
    CCAGCCTCAG GCACAATACC ACTGAATTCG ATGAACGTGG ATGCAGTTTG TGAGAAACTG
    AAACAAATTG AAGGGCTGGA CCAGAGCATG CTGCCTCAGT ACTGTGCAAC CATCAAAAAG
    GCGAACATAA ATGGCCGTGT GTTGGCTCAG TGTAACATTG ATGAACTGAA GAAGGAAATG
    AATATGAATT TTGGAGACTG GCATCTTTTT AGAAGCACAA TATTAGAAAT GAGAAATGCA
    GAAAACCAAG TGGTCCCTGA AGACCCACGT TTACTGAGTG AAAACAGCAG CTCTGTTCCT
    CACGGTGAAC CTGCTCGTCG CTCCGCACAC AGCGAGCTGC CCCACACCGA GCTCTCCAGT
    CAGGCTCCCT ACACCCTGAA CTTCAGCTTT GAAGAGCTGA ACACGCTCGG CCTGGACGAA
    GGGGCTCCTC GCCACAGCAA CCTGAGTTGG CAGAAACTTC ATGTTGTGCA AGTTGAAAAA
    GGTTCTTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855322.1
    CDS213..4244
    Translation

    Target ORF information:

    RefSeq Version XM_024999554.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999554.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGGATCAGGC
    CCAGCCTCAG GCACAATACC ACTGAATTCG ATGAACGTGG ATGCAGTTTG TGAGAAACTG
    AAACAAATTG AAGGGCTGGA CCAGAGCATG CTGCCTCAGT ACTGTGCAAC CATCAAAAAG
    GCGAACATAA ATGGCCGTGT GTTGGCTCAG TGTAACATTG ATGAACTGAA GAAGGAAATG
    AATATGAATT TTGGAGACTG GCATCTTTTT AGAAGCACAA TATTAGAAAT GAGAAATGCA
    GAAAACCAAG TGGTCCCTGA AGACCCACGT TTACTGAGTG AAAACAGCAG CTCTGTTCCT
    CACGGTGAAC CTGCTCGTCG CTCCGCACAC AGCGAGCTGC CCCACACCGA GCTCTCCAGT
    CAGGCTCCCT ACACCCTGAA CTTCAGCTTT GAAGAGCTGA ACACGCTCGG CCTGGACGAA
    GGGGCTCCTC GCCACAGCAA CCTGAGTTGG CAGAAACTTC ATGTTGTGCA AGTTGAAAAA
    GGTTCTTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53366
    Clone ID Related Accession (Same CDS sequence) XM_015465446.1 , XM_010810309.2 , XM_015465445.1 , XM_010810308.2
    Accession Version XM_015465446.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGAAGAAA ATGCTGCCTT TGAGATAGAT AGATCTTCCG AGGGATCCAG TTTAAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320932.1
    CDS209..4468
    Translation

    Target ORF information:

    RefSeq Version XM_015465446.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465446.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGAAGAAA ATGCTGCCTT TGAGATAGAT AGATCTTCCG AGGGATCCAG TTTAAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53366
    Clone ID Related Accession (Same CDS sequence) XM_015465446.1 , XM_010810309.2 , XM_015465445.1 , XM_010810308.2
    Accession Version XM_010810309.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810309.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGAAGAAA ATGCTGCCTT TGAGATAGAT AGATCTTCCG AGGGATCCAG TTTAAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808611.1
    CDS209..4468
    Translation

    Target ORF information:

    RefSeq Version XM_010810309.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810309.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGAAGAAA ATGCTGCCTT TGAGATAGAT AGATCTTCCG AGGGATCCAG TTTAAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53366
    Clone ID Related Accession (Same CDS sequence) XM_015465446.1 , XM_010810309.2 , XM_015465445.1 , XM_010810308.2
    Accession Version XM_015465445.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGAAGAAA ATGCTGCCTT TGAGATAGAT AGATCTTCCG AGGGATCCAG TTTAAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320931.1
    CDS209..4468
    Translation

    Target ORF information:

    RefSeq Version XM_015465445.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465445.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGAAGAAA ATGCTGCCTT TGAGATAGAT AGATCTTCCG AGGGATCCAG TTTAAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53366
    Clone ID Related Accession (Same CDS sequence) XM_015465446.1 , XM_010810309.2 , XM_015465445.1 , XM_010810308.2
    Accession Version XM_010810308.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810308.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGAAGAAA ATGCTGCCTT TGAGATAGAT AGATCTTCCG AGGGATCCAG TTTAAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808610.1
    CDS209..4468
    Translation

    Target ORF information:

    RefSeq Version XM_010810308.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810308.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGAAGAAA ATGCTGCCTT TGAGATAGAT AGATCTTCCG AGGGATCCAG TTTAAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220911
    Clone ID Related Accession (Same CDS sequence) XM_024999549.1
    Accession Version XM_024999549.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGAAGTTATC
    AAGGAGGATG CTGCTGAGGG CCTTTCTTCA CCCACAGACT CCTCGAGGCC GTGTGACTCT
    GGGTTTAACA AACAGAGACA GGGATCAGGC CCAGCCTCAG GCACAATACC ACTGAATTCG
    ATGAACGTGG ATGCAGTTTG TGAGAAACTG AAACAAATTG AAGGGCTGGA CCAGAGCATG
    CTGCCTCAGT ACTGTGCAAC CATCAAAAAG GCGAACATAA ATGGCCGTGT GTTGGCTCAG
    TGTAACATTG ATGAACTGAA GAAGGAAATG AATATGAATT TTGGAGACTG GCATCTTTTT
    AGAAGCACAA TATTAGAAAT GAGAAATGCA GAAAACCAAG TGGTCCCTGA AGACCCACGT
    TTACTGAGTG AAAACAGCAG CTCTGTTCCT CACGGTGAAC CTGCTCGTCG CTCCGCACAC
    AGCGAGCTGC CCCACACCGA GCTCTCCAGT CAGGCTCCCT ACACCCTGAA CTTCAGCTTT
    GAAGAGCTGA ACACGCTCGG CCTGGACGAA GGGGCTCCTC GCCACAGCAA CCTGAGTTGG
    CAGTCACAAA CTCGCAGAAC CCCAAGTCTT TCCAGTCTCA CTTCCCAGGA CTCCAGTATT
    GAAATTTCAA AGCTTACTGA TAAGGTGCAG GCTGAGTATC GAGACGCTTA TAGAGAGTAC
    ATTGCTCAGA TGTCTCAGTT AGAAGGGGGC ACAGGTTCCA CAACCATGAG TGGCAGGTCT
    TCTCCACACA GCACGTATTA TATGGGTCAG AGTTCATCCG GGGGCTCCAT TCACTCTAGT
    CTAGAACCCG AGAAAGGGAA GGATAGTGAA CCAAAGCAAG ATGATACACG GAAGTCATTT
    CTGATGAAGA GGGGAGATGT TATAGATTAT TCCTCATCAG GAGTTTCCAC CAATGATGCC
    TCACCCCTGG ATCCTATTAC CGAGGAAGAT GAAAAATCTG ATCAGTCAGG CAGTAAGCTT
    CTCCCAGGCA AGAAGTCCTC AGAAAGGTCA AGTCTCTTCC AGACAGACCT GAAGCTGAAG
    GGAGGTGGAC TGCGCTATCA GAAACTCCCG AGTGACGAAG ACGAGTCTGG GACAGAAGAG
    TCAGACAACA CTCCACTGCT CAAAGACGAC AAAGACAAGA AAGCTGAAGG GAAAGCAGAG
    AGAGCATTGA AGTCTCCAGA GCACAGTGCA GAGCCCATTC GAACCTTCAT CAAGGCCAAA
    GAGTACTTGT CAGATGCCCT CCTTGACAAA AAGGACTCCT CTGACTCAGG AGTGAGGTCC
    AATGAGAGCT CGCCCAACCA CTCCCTTCAC AACGAGGCGG CAGATGACTC CCAGCTTGAA
    AAGGCCAACC TCATAGAGCT CGAAGATGAC CCCCACAGTG GAAGACGGGG AGTCCCGCAC
    AGCCTGAGTG GCTTGCCAGA CCCCCTCGTA GCCCGGATGT CCATTTGCTC CGAGGACAAG
    AAGAGCCCTT CTGAATGCAG CTTGATAGCC AGCAGCCCCG AGGAAAACTG GCCAGCATGC
    CACAAATCCT ACAACCTGAA TCGCACCCCC AGCACCGTGA CCCTGAACAA CAACAGTGCC
    CCGGCTAACA GAGCCAACCA GAATTCCGAT GAGACGGAGG GGCTGAGGGA GACGCCTCAA
    GTCCTCCTGA GGTCAGGCTC CAGTTCCCAC TCCACCGCTC TGCAGAATGA GAACCTGAAG
    AGCATGGCAC ACAAGCGAGG CCAGCGTTCC AGCTACACGA GGCTCTCAAA GGACTCCTCA
    GAGCTCCACG CAGTAGCCTC CTCCGATAGT ACAGGTTTTG GAGAAGAAAG GGAGAGCATT
    CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855317.1
    CDS213..5558
    Translation

    Target ORF information:

    RefSeq Version XM_024999549.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999549.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGAAGTTATC
    AAGGAGGATG CTGCTGAGGG CCTTTCTTCA CCCACAGACT CCTCGAGGCC GTGTGACTCT
    GGGTTTAACA AACAGAGACA GGGATCAGGC CCAGCCTCAG GCACAATACC ACTGAATTCG
    ATGAACGTGG ATGCAGTTTG TGAGAAACTG AAACAAATTG AAGGGCTGGA CCAGAGCATG
    CTGCCTCAGT ACTGTGCAAC CATCAAAAAG GCGAACATAA ATGGCCGTGT GTTGGCTCAG
    TGTAACATTG ATGAACTGAA GAAGGAAATG AATATGAATT TTGGAGACTG GCATCTTTTT
    AGAAGCACAA TATTAGAAAT GAGAAATGCA GAAAACCAAG TGGTCCCTGA AGACCCACGT
    TTACTGAGTG AAAACAGCAG CTCTGTTCCT CACGGTGAAC CTGCTCGTCG CTCCGCACAC
    AGCGAGCTGC CCCACACCGA GCTCTCCAGT CAGGCTCCCT ACACCCTGAA CTTCAGCTTT
    GAAGAGCTGA ACACGCTCGG CCTGGACGAA GGGGCTCCTC GCCACAGCAA CCTGAGTTGG
    CAGTCACAAA CTCGCAGAAC CCCAAGTCTT TCCAGTCTCA CTTCCCAGGA CTCCAGTATT
    GAAATTTCAA AGCTTACTGA TAAGGTGCAG GCTGAGTATC GAGACGCTTA TAGAGAGTAC
    ATTGCTCAGA TGTCTCAGTT AGAAGGGGGC ACAGGTTCCA CAACCATGAG TGGCAGGTCT
    TCTCCACACA GCACGTATTA TATGGGTCAG AGTTCATCCG GGGGCTCCAT TCACTCTAGT
    CTAGAACCCG AGAAAGGGAA GGATAGTGAA CCAAAGCAAG ATGATACACG GAAGTCATTT
    CTGATGAAGA GGGGAGATGT TATAGATTAT TCCTCATCAG GAGTTTCCAC CAATGATGCC
    TCACCCCTGG ATCCTATTAC CGAGGAAGAT GAAAAATCTG ATCAGTCAGG CAGTAAGCTT
    CTCCCAGGCA AGAAGTCCTC AGAAAGGTCA AGTCTCTTCC AGACAGACCT GAAGCTGAAG
    GGAGGTGGAC TGCGCTATCA GAAACTCCCG AGTGACGAAG ACGAGTCTGG GACAGAAGAG
    TCAGACAACA CTCCACTGCT CAAAGACGAC AAAGACAAGA AAGCTGAAGG GAAAGCAGAG
    AGAGCATTGA AGTCTCCAGA GCACAGTGCA GAGCCCATTC GAACCTTCAT CAAGGCCAAA
    GAGTACTTGT CAGATGCCCT CCTTGACAAA AAGGACTCCT CTGACTCAGG AGTGAGGTCC
    AATGAGAGCT CGCCCAACCA CTCCCTTCAC AACGAGGCGG CAGATGACTC CCAGCTTGAA
    AAGGCCAACC TCATAGAGCT CGAAGATGAC CCCCACAGTG GAAGACGGGG AGTCCCGCAC
    AGCCTGAGTG GCTTGCCAGA CCCCCTCGTA GCCCGGATGT CCATTTGCTC CGAGGACAAG
    AAGAGCCCTT CTGAATGCAG CTTGATAGCC AGCAGCCCCG AGGAAAACTG GCCAGCATGC
    CACAAATCCT ACAACCTGAA TCGCACCCCC AGCACCGTGA CCCTGAACAA CAACAGTGCC
    CCGGCTAACA GAGCCAACCA GAATTCCGAT GAGACGGAGG GGCTGAGGGA GACGCCTCAA
    GTCCTCCTGA GGTCAGGCTC CAGTTCCCAC TCCACCGCTC TGCAGAATGA GAACCTGAAG
    AGCATGGCAC ACAAGCGAGG CCAGCGTTCC AGCTACACGA GGCTCTCAAA GGACTCCTCA
    GAGCTCCACG CAGTAGCCTC CTCCGATAGT ACAGGTTTTG GAGAAGAAAG GGAGAGCATT
    CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220912
    Clone ID Related Accession (Same CDS sequence) XM_024999550.1
    Accession Version XM_024999550.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGGATCAGGC
    CCAGCCTCAG GCACAATACC ACTGAATTCG ATGAACGTGG ATGCAGTTTG TGAGAAACTG
    AAACAAATTG AAGGGCTGGA CCAGAGCATG CTGCCTCAGT ACTGTGCAAC CATCAAAAAG
    GCGAACATAA ATGGCCGTGT GTTGGCTCAG TGTAACATTG ATGAACTGAA GAAGGAAATG
    AATATGAATT TTGGAGACTG GCATCTTTTT AGAAGCACAA TATTAGAAAT GAGAAATGCA
    GAAAACCAAG TGGTCCCTGA AGACCCACGT TTACTGAGTG AAAACAGCAG CTCTGTTCCT
    CACGGTGAAC CTGCTCGTCG CTCCGCACAC AGCGAGCTGC CCCACACCGA GCTCTCCAGT
    CAGGCTCCCT ACACCCTGAA CTTCAGCTTT GAAGAGCTGA ACACGCTCGG CCTGGACGAA
    GGGGCTCCTC GCCACAGCAA CCTGAGTTGG CAGTCACAAA CTCGCAGAAC CCCAAGTCTT
    TCCAGTCTCA CTTCCCAGGA CTCCAGTATT GAAATTTCAA AGCTTACTGA TAAGGTGCAG
    GCTGAGTATC GAGACGCTTA TAGAGAGTAC ATTGCTCAGA TGTCTCAGTT AGAAGGGGGC
    ACAGGTTCCA CAACCATGAG TGGCAGGTCT TCTCCACACA GCACGTATTA TATGGGTCAG
    AGTTCATCCG GGGGCTCCAT TCACTCTAGT CTAGAACCCG AGAAAGGGAA GGATAGTGAA
    CCAAAGCAAG ATGATACACG GAAGTCATTT CTGATGAAGA GGGGAGATGT TATAGATTAT
    TCCTCATCAG GAGTTTCCAC CAATGATGCC TCACCCCTGG ATCCTATTAC CGAGGAAGAT
    GAAAAATCTG ATCAGTCAGG CAGTAAGCTT CTCCCAGGCA AGAAGTCCTC AGAAAGGTCA
    AGTCTCTTCC AGACAGACCT GAAGCTGAAG GGAGGTGGAC TGCGCTATCA GAAACTCCCG
    AGTGACGAAG ACGAGTCTGG GACAGAAGAG TCAGACAACA CTCCACTGCT CAAAGACGAC
    AAAGACAAGA AAGCTGAAGG GAAAGCAGAG AGAGCATTGA AGTCTCCAGA GCACAGTGCA
    GAGCCCATTC GAACCTTCAT CAAGGCCAAA GAGTACTTGT CAGATGCCCT CCTTGACAAA
    AAGGACTCCT CTGACTCAGG AGTGAGGTCC AATGAGAGCT CGCCCAACCA CTCCCTTCAC
    AACGAGGCGG CAGATGACTC CCAGCTTGAA AAGGCCAACC TCATAGAGCT CGAAGATGAC
    CCCCACAGTG GAAGACGGGG AGTCCCGCAC AGCCTGAGTG GCTTGCCAGA CCCCCTCGTA
    GCCCGGATGT CCATTTGCTC CGAGGACAAG AAGAGCCCTT CTGAATGCAG CTTGATAGCC
    AGCAGCCCCG AGGAAAACTG GCCAGCATGC CACAAATCCT ACAACCTGAA TCGCACCCCC
    AGCACCGTGA CCCTGAACAA CAACAGTGCC CCGGCTAACA GAGCCAACCA GAATTCCGAT
    GAGACGGAGG GGCTGAGGGA GACGCCTCAA GTCCTCCTGA GGTCAGGCTC CAGTTCCCAC
    TCCACCGCTC TGCAGAATGA GAACCTGAAG AGCATGGCAC ACAAGCGAGG CCAGCGTTCC
    AGCTACACGA GGCTCTCAAA GGACTCCTCA GAGCTCCACG CAGTAGCCTC CTCCGATAGT
    ACAGGTTTTG GAGAAGAAAG GGAGAGCATT CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855318.1
    CDS213..5468
    Translation

    Target ORF information:

    RefSeq Version XM_024999550.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999550.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGGATCAGGC
    CCAGCCTCAG GCACAATACC ACTGAATTCG ATGAACGTGG ATGCAGTTTG TGAGAAACTG
    AAACAAATTG AAGGGCTGGA CCAGAGCATG CTGCCTCAGT ACTGTGCAAC CATCAAAAAG
    GCGAACATAA ATGGCCGTGT GTTGGCTCAG TGTAACATTG ATGAACTGAA GAAGGAAATG
    AATATGAATT TTGGAGACTG GCATCTTTTT AGAAGCACAA TATTAGAAAT GAGAAATGCA
    GAAAACCAAG TGGTCCCTGA AGACCCACGT TTACTGAGTG AAAACAGCAG CTCTGTTCCT
    CACGGTGAAC CTGCTCGTCG CTCCGCACAC AGCGAGCTGC CCCACACCGA GCTCTCCAGT
    CAGGCTCCCT ACACCCTGAA CTTCAGCTTT GAAGAGCTGA ACACGCTCGG CCTGGACGAA
    GGGGCTCCTC GCCACAGCAA CCTGAGTTGG CAGTCACAAA CTCGCAGAAC CCCAAGTCTT
    TCCAGTCTCA CTTCCCAGGA CTCCAGTATT GAAATTTCAA AGCTTACTGA TAAGGTGCAG
    GCTGAGTATC GAGACGCTTA TAGAGAGTAC ATTGCTCAGA TGTCTCAGTT AGAAGGGGGC
    ACAGGTTCCA CAACCATGAG TGGCAGGTCT TCTCCACACA GCACGTATTA TATGGGTCAG
    AGTTCATCCG GGGGCTCCAT TCACTCTAGT CTAGAACCCG AGAAAGGGAA GGATAGTGAA
    CCAAAGCAAG ATGATACACG GAAGTCATTT CTGATGAAGA GGGGAGATGT TATAGATTAT
    TCCTCATCAG GAGTTTCCAC CAATGATGCC TCACCCCTGG ATCCTATTAC CGAGGAAGAT
    GAAAAATCTG ATCAGTCAGG CAGTAAGCTT CTCCCAGGCA AGAAGTCCTC AGAAAGGTCA
    AGTCTCTTCC AGACAGACCT GAAGCTGAAG GGAGGTGGAC TGCGCTATCA GAAACTCCCG
    AGTGACGAAG ACGAGTCTGG GACAGAAGAG TCAGACAACA CTCCACTGCT CAAAGACGAC
    AAAGACAAGA AAGCTGAAGG GAAAGCAGAG AGAGCATTGA AGTCTCCAGA GCACAGTGCA
    GAGCCCATTC GAACCTTCAT CAAGGCCAAA GAGTACTTGT CAGATGCCCT CCTTGACAAA
    AAGGACTCCT CTGACTCAGG AGTGAGGTCC AATGAGAGCT CGCCCAACCA CTCCCTTCAC
    AACGAGGCGG CAGATGACTC CCAGCTTGAA AAGGCCAACC TCATAGAGCT CGAAGATGAC
    CCCCACAGTG GAAGACGGGG AGTCCCGCAC AGCCTGAGTG GCTTGCCAGA CCCCCTCGTA
    GCCCGGATGT CCATTTGCTC CGAGGACAAG AAGAGCCCTT CTGAATGCAG CTTGATAGCC
    AGCAGCCCCG AGGAAAACTG GCCAGCATGC CACAAATCCT ACAACCTGAA TCGCACCCCC
    AGCACCGTGA CCCTGAACAA CAACAGTGCC CCGGCTAACA GAGCCAACCA GAATTCCGAT
    GAGACGGAGG GGCTGAGGGA GACGCCTCAA GTCCTCCTGA GGTCAGGCTC CAGTTCCCAC
    TCCACCGCTC TGCAGAATGA GAACCTGAAG AGCATGGCAC ACAAGCGAGG CCAGCGTTCC
    AGCTACACGA GGCTCTCAAA GGACTCCTCA GAGCTCCACG CAGTAGCCTC CTCCGATAGT
    ACAGGTTTTG GAGAAGAAAG GGAGAGCATT CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220913
    Clone ID Related Accession (Same CDS sequence) XM_024999551.1
    Accession Version XM_024999551.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGGGA
    TCAGGCCCAG CCTCAGGCAC AATACCACTG AATTCGATGA ACGTGGATGC AGTTTGTGAG
    AAACTGAAAC AAATTGAAGG GCTGGACCAG AGCATGCTGC CTCAGTACTG TGCAACCATC
    AAAAAGGCGA ACATAAATGG CCGTGTGTTG GCTCAGTGTA ACATTGATGA ACTGAAGAAG
    GAAATGAATA TGAATTTTGG AGACTGGCAT CTTTTTAGAA GCACAATATT AGAAATGAGA
    AATGCAGAAA ACCAAGTGGT CCCTGAAGAC CCACGTTTAC TGAGTGAAAA CAGCAGCTCT
    GTTCCTCACG GTGAACCTGC TCGTCGCTCC GCACACAGCG AGCTGCCCCA CACCGAGCTC
    TCCAGTCAGG CTCCCTACAC CCTGAACTTC AGCTTTGAAG AGCTGAACAC GCTCGGCCTG
    GACGAAGGGG CTCCTCGCCA CAGCAACCTG AGTTGGCAGT CACAAACTCG CAGAACCCCA
    AGTCTTTCCA GTCTCACTTC CCAGGACTCC AGTATTGAAA TTTCAAAGCT TACTGATAAG
    GTGCAGGCTG AGTATCGAGA CGCTTATAGA GAGTACATTG CTCAGATGTC TCAGTTAGAA
    GGGGGCACAG GTTCCACAAC CATGAGTGGC AGGTCTTCTC CACACAGCAC GTATTATATG
    GGTCAGAGTT CATCCGGGGG CTCCATTCAC TCTAGTCTAG AACCCGAGAA AGGGAAGGAT
    AGTGAACCAA AGCAAGATGA TACACGGAAG TCATTTCTGA TGAAGAGGGG AGATGTTATA
    GATTATTCCT CATCAGGAGT TTCCACCAAT GATGCCTCAC CCCTGGATCC TATTACCGAG
    GAAGATGAAA AATCTGATCA GTCAGGCAGT AAGCTTCTCC CAGGCAAGAA GTCCTCAGAA
    AGGTCAAGTC TCTTCCAGAC AGACCTGAAG CTGAAGGGAG GTGGACTGCG CTATCAGAAA
    CTCCCGAGTG ACGAAGACGA GTCTGGGACA GAAGAGTCAG ACAACACTCC ACTGCTCAAA
    GACGACAAAG ACAAGAAAGC TGAAGGGAAA GCAGAGAGAG CATTGAAGTC TCCAGAGCAC
    AGTGCAGAGC CCATTCGAAC CTTCATCAAG GCCAAAGAGT ACTTGTCAGA TGCCCTCCTT
    GACAAAAAGG ACTCCTCTGA CTCAGGAGTG AGGTCCAATG AGAGCTCGCC CAACCACTCC
    CTTCACAACG AGGCGGCAGA TGACTCCCAG CTTGAAAAGG CCAACCTCAT AGAGCTCGAA
    GATGACCCCC ACAGTGGAAG ACGGGGAGTC CCGCACAGCC TGAGTGGCTT GCCAGACCCC
    CTCGTAGCCC GGATGTCCAT TTGCTCCGAG GACAAGAAGA GCCCTTCTGA ATGCAGCTTG
    ATAGCCAGCA GCCCCGAGGA AAACTGGCCA GCATGCCACA AATCCTACAA CCTGAATCGC
    ACCCCCAGCA CCGTGACCCT GAACAACAAC AGTGCCCCGG CTAACAGAGC CAACCAGAAT
    TCCGATGAGA CGGAGGGGCT GAGGGAGACG CCTCAAGTCC TCCTGAGGTC AGGCTCCAGT
    TCCCACTCCA CCGCTCTGCA GAATGAGAAC CTGAAGAGCA TGGCACACAA GCGAGGCCAG
    CGTTCCAGCT ACACGAGGCT CTCAAAGGAC TCCTCAGAGC TCCACGCAGT AGCCTCCTCC
    GATAGTACAG GTTTTGGAGA AGAAAGGGAG AGCATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855319.1
    CDS213..5354
    Translation

    Target ORF information:

    RefSeq Version XM_024999551.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999551.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGGGA
    TCAGGCCCAG CCTCAGGCAC AATACCACTG AATTCGATGA ACGTGGATGC AGTTTGTGAG
    AAACTGAAAC AAATTGAAGG GCTGGACCAG AGCATGCTGC CTCAGTACTG TGCAACCATC
    AAAAAGGCGA ACATAAATGG CCGTGTGTTG GCTCAGTGTA ACATTGATGA ACTGAAGAAG
    GAAATGAATA TGAATTTTGG AGACTGGCAT CTTTTTAGAA GCACAATATT AGAAATGAGA
    AATGCAGAAA ACCAAGTGGT CCCTGAAGAC CCACGTTTAC TGAGTGAAAA CAGCAGCTCT
    GTTCCTCACG GTGAACCTGC TCGTCGCTCC GCACACAGCG AGCTGCCCCA CACCGAGCTC
    TCCAGTCAGG CTCCCTACAC CCTGAACTTC AGCTTTGAAG AGCTGAACAC GCTCGGCCTG
    GACGAAGGGG CTCCTCGCCA CAGCAACCTG AGTTGGCAGT CACAAACTCG CAGAACCCCA
    AGTCTTTCCA GTCTCACTTC CCAGGACTCC AGTATTGAAA TTTCAAAGCT TACTGATAAG
    GTGCAGGCTG AGTATCGAGA CGCTTATAGA GAGTACATTG CTCAGATGTC TCAGTTAGAA
    GGGGGCACAG GTTCCACAAC CATGAGTGGC AGGTCTTCTC CACACAGCAC GTATTATATG
    GGTCAGAGTT CATCCGGGGG CTCCATTCAC TCTAGTCTAG AACCCGAGAA AGGGAAGGAT
    AGTGAACCAA AGCAAGATGA TACACGGAAG TCATTTCTGA TGAAGAGGGG AGATGTTATA
    GATTATTCCT CATCAGGAGT TTCCACCAAT GATGCCTCAC CCCTGGATCC TATTACCGAG
    GAAGATGAAA AATCTGATCA GTCAGGCAGT AAGCTTCTCC CAGGCAAGAA GTCCTCAGAA
    AGGTCAAGTC TCTTCCAGAC AGACCTGAAG CTGAAGGGAG GTGGACTGCG CTATCAGAAA
    CTCCCGAGTG ACGAAGACGA GTCTGGGACA GAAGAGTCAG ACAACACTCC ACTGCTCAAA
    GACGACAAAG ACAAGAAAGC TGAAGGGAAA GCAGAGAGAG CATTGAAGTC TCCAGAGCAC
    AGTGCAGAGC CCATTCGAAC CTTCATCAAG GCCAAAGAGT ACTTGTCAGA TGCCCTCCTT
    GACAAAAAGG ACTCCTCTGA CTCAGGAGTG AGGTCCAATG AGAGCTCGCC CAACCACTCC
    CTTCACAACG AGGCGGCAGA TGACTCCCAG CTTGAAAAGG CCAACCTCAT AGAGCTCGAA
    GATGACCCCC ACAGTGGAAG ACGGGGAGTC CCGCACAGCC TGAGTGGCTT GCCAGACCCC
    CTCGTAGCCC GGATGTCCAT TTGCTCCGAG GACAAGAAGA GCCCTTCTGA ATGCAGCTTG
    ATAGCCAGCA GCCCCGAGGA AAACTGGCCA GCATGCCACA AATCCTACAA CCTGAATCGC
    ACCCCCAGCA CCGTGACCCT GAACAACAAC AGTGCCCCGG CTAACAGAGC CAACCAGAAT
    TCCGATGAGA CGGAGGGGCT GAGGGAGACG CCTCAAGTCC TCCTGAGGTC AGGCTCCAGT
    TCCCACTCCA CCGCTCTGCA GAATGAGAAC CTGAAGAGCA TGGCACACAA GCGAGGCCAG
    CGTTCCAGCT ACACGAGGCT CTCAAAGGAC TCCTCAGAGC TCCACGCAGT AGCCTCCTCC
    GATAGTACAG GTTTTGGAGA AGAAAGGGAG AGCATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24271
    Clone ID Related Accession (Same CDS sequence) XM_005213101.3 , XM_015465440.1 , XM_005213101.4 , XM_024999548.1
    Accession Version XM_005213101.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213101.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GCCGTGTGAC
    TCTGGGTTTA ACAAACAGAG ACAGGGATCA GGCCCAGCCT CAGGCACAAT ACCACTGAAT
    TCGATGAACG TGGATGCAGT TTGTGAGAAA CTGAAACAAA TTGAAGGGCT GGACCAGAGC
    ATGCTGCCTC AGTACTGTGC AACCATCAAA AAGGCGAACA TAAATGGCCG TGTGTTGGCT
    CAGTGTAACA TTGATGAACT GAAGAAGGAA ATGAATATGA ATTTTGGAGA CTGGCATCTT
    TTTAGAAGCA CAATATTAGA AATGAGAAAT GCAGAAAACC AAGTGGTCCC TGAAGACCCA
    CGTTTACTGA GTGAAAACAG CAGCTCTGTT CCTCACGGTG AACCTGCTCG TCGCTCCGCA
    CACAGCGAGC TGCCCCACAC CGAGCTCTCC AGTCAGGCTC CCTACACCCT GAACTTCAGC
    TTTGAAGAGC TGAACACGCT CGGCCTGGAC GAAGGGGCTC CTCGCCACAG CAACCTGAGT
    TGGCAGTCAC AAACTCGCAG AACCCCAAGT CTTTCCAGTC TCACTTCCCA GGACTCCAGT
    ATTGAAATTT CAAAGCTTAC TGATAAGGTG CAGGCTGAGT ATCGAGACGC TTATAGAGAG
    TACATTGCTC AGATGTCTCA GTTAGAAGGG GGCACAGGTT CCACAACCAT GAGTGGCAGG
    TCTTCTCCAC ACAGCACGTA TTATATGGGT CAGAGTTCAT CCGGGGGCTC CATTCACTCT
    AGTCTAGAAC CCGAGAAAGG GAAGGATAGT GAACCAAAGC AAGATGATAC ACGGAAGTCA
    TTTCTGATGA AGAGGGGAGA TGTTATAGAT TATTCCTCAT CAGGAGTTTC CACCAATGAT
    GCCTCACCCC TGGATCCTAT TACCGAGGAA GATGAAAAAT CTGATCAGTC AGGCAGTAAG
    CTTCTCCCAG GCAAGAAGTC CTCAGAAAGG TCAAGTCTCT TCCAGACAGA CCTGAAGCTG
    AAGGGAGGTG GACTGCGCTA TCAGAAACTC CCGAGTGACG AAGACGAGTC TGGGACAGAA
    GAGTCAGACA ACACTCCACT GCTCAAAGAC GACAAAGACA AGAAAGCTGA AGGGAAAGCA
    GAGAGAGCAT TGAAGTCTCC AGAGCACAGT GCAGAGCCCA TTCGAACCTT CATCAAGGCC
    AAAGAGTACT TGTCAGATGC CCTCCTTGAC AAAAAGGACT CCTCTGACTC AGGAGTGAGG
    TCCAATGAGA GCTCGCCCAA CCACTCCCTT CACAACGAGG CGGCAGATGA CTCCCAGCTT
    GAAAAGGCCA ACCTCATAGA GCTCGAAGAT GACCCCCACA GTGGAAGACG GGGAGTCCCG
    CACAGCCTGA GTGGCTTGCC AGACCCCCTC GTAGCCCGGA TGTCCATTTG CTCCGAGGAC
    AAGAAGAGCC CTTCTGAATG CAGCTTGATA GCCAGCAGCC CCGAGGAAAA CTGGCCAGCA
    TGCCACAAAT CCTACAACCT GAATCGCACC CCCAGCACCG TGACCCTGAA CAACAACAGT
    GCCCCGGCTA ACAGAGCCAA CCAGAATTCC GATGAGACGG AGGGGCTGAG GGAGACGCCT
    CAAGTCCTCC TGAGGTCAGG CTCCAGTTCC CACTCCACCG CTCTGCAGAA TGAGAACCTG
    AAGAGCATGG CACACAAGCG AGGCCAGCGT TCCAGCTACA CGAGGCTCTC AAAGGACTCC
    TCAGAGCTCC ACGCAGTAGC CTCCTCCGAT AGTACAGGTT TTGGAGAAGA AAGGGAGAGC
    ATTCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213158.1
    CDS207..5555
    Translation

    Target ORF information:

    RefSeq Version XM_005213101.3
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213101.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GCCGTGTGAC
    TCTGGGTTTA ACAAACAGAG ACAGGGATCA GGCCCAGCCT CAGGCACAAT ACCACTGAAT
    TCGATGAACG TGGATGCAGT TTGTGAGAAA CTGAAACAAA TTGAAGGGCT GGACCAGAGC
    ATGCTGCCTC AGTACTGTGC AACCATCAAA AAGGCGAACA TAAATGGCCG TGTGTTGGCT
    CAGTGTAACA TTGATGAACT GAAGAAGGAA ATGAATATGA ATTTTGGAGA CTGGCATCTT
    TTTAGAAGCA CAATATTAGA AATGAGAAAT GCAGAAAACC AAGTGGTCCC TGAAGACCCA
    CGTTTACTGA GTGAAAACAG CAGCTCTGTT CCTCACGGTG AACCTGCTCG TCGCTCCGCA
    CACAGCGAGC TGCCCCACAC CGAGCTCTCC AGTCAGGCTC CCTACACCCT GAACTTCAGC
    TTTGAAGAGC TGAACACGCT CGGCCTGGAC GAAGGGGCTC CTCGCCACAG CAACCTGAGT
    TGGCAGTCAC AAACTCGCAG AACCCCAAGT CTTTCCAGTC TCACTTCCCA GGACTCCAGT
    ATTGAAATTT CAAAGCTTAC TGATAAGGTG CAGGCTGAGT ATCGAGACGC TTATAGAGAG
    TACATTGCTC AGATGTCTCA GTTAGAAGGG GGCACAGGTT CCACAACCAT GAGTGGCAGG
    TCTTCTCCAC ACAGCACGTA TTATATGGGT CAGAGTTCAT CCGGGGGCTC CATTCACTCT
    AGTCTAGAAC CCGAGAAAGG GAAGGATAGT GAACCAAAGC AAGATGATAC ACGGAAGTCA
    TTTCTGATGA AGAGGGGAGA TGTTATAGAT TATTCCTCAT CAGGAGTTTC CACCAATGAT
    GCCTCACCCC TGGATCCTAT TACCGAGGAA GATGAAAAAT CTGATCAGTC AGGCAGTAAG
    CTTCTCCCAG GCAAGAAGTC CTCAGAAAGG TCAAGTCTCT TCCAGACAGA CCTGAAGCTG
    AAGGGAGGTG GACTGCGCTA TCAGAAACTC CCGAGTGACG AAGACGAGTC TGGGACAGAA
    GAGTCAGACA ACACTCCACT GCTCAAAGAC GACAAAGACA AGAAAGCTGA AGGGAAAGCA
    GAGAGAGCAT TGAAGTCTCC AGAGCACAGT GCAGAGCCCA TTCGAACCTT CATCAAGGCC
    AAAGAGTACT TGTCAGATGC CCTCCTTGAC AAAAAGGACT CCTCTGACTC AGGAGTGAGG
    TCCAATGAGA GCTCGCCCAA CCACTCCCTT CACAACGAGG CGGCAGATGA CTCCCAGCTT
    GAAAAGGCCA ACCTCATAGA GCTCGAAGAT GACCCCCACA GTGGAAGACG GGGAGTCCCG
    CACAGCCTGA GTGGCTTGCC AGACCCCCTC GTAGCCCGGA TGTCCATTTG CTCCGAGGAC
    AAGAAGAGCC CTTCTGAATG CAGCTTGATA GCCAGCAGCC CCGAGGAAAA CTGGCCAGCA
    TGCCACAAAT CCTACAACCT GAATCGCACC CCCAGCACCG TGACCCTGAA CAACAACAGT
    GCCCCGGCTA ACAGAGCCAA CCAGAATTCC GATGAGACGG AGGGGCTGAG GGAGACGCCT
    CAAGTCCTCC TGAGGTCAGG CTCCAGTTCC CACTCCACCG CTCTGCAGAA TGAGAACCTG
    AAGAGCATGG CACACAAGCG AGGCCAGCGT TCCAGCTACA CGAGGCTCTC AAAGGACTCC
    TCAGAGCTCC ACGCAGTAGC CTCCTCCGAT AGTACAGGTT TTGGAGAAGA AAGGGAGAGC
    ATTCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24271
    Clone ID Related Accession (Same CDS sequence) XM_005213101.3 , XM_015465440.1 , XM_005213101.4 , XM_024999548.1
    Accession Version XM_015465440.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GCCGTGTGAC
    TCTGGGTTTA ACAAACAGAG ACAGGGATCA GGCCCAGCCT CAGGCACAAT ACCACTGAAT
    TCGATGAACG TGGATGCAGT TTGTGAGAAA CTGAAACAAA TTGAAGGGCT GGACCAGAGC
    ATGCTGCCTC AGTACTGTGC AACCATCAAA AAGGCGAACA TAAATGGCCG TGTGTTGGCT
    CAGTGTAACA TTGATGAACT GAAGAAGGAA ATGAATATGA ATTTTGGAGA CTGGCATCTT
    TTTAGAAGCA CAATATTAGA AATGAGAAAT GCAGAAAACC AAGTGGTCCC TGAAGACCCA
    CGTTTACTGA GTGAAAACAG CAGCTCTGTT CCTCACGGTG AACCTGCTCG TCGCTCCGCA
    CACAGCGAGC TGCCCCACAC CGAGCTCTCC AGTCAGGCTC CCTACACCCT GAACTTCAGC
    TTTGAAGAGC TGAACACGCT CGGCCTGGAC GAAGGGGCTC CTCGCCACAG CAACCTGAGT
    TGGCAGTCAC AAACTCGCAG AACCCCAAGT CTTTCCAGTC TCACTTCCCA GGACTCCAGT
    ATTGAAATTT CAAAGCTTAC TGATAAGGTG CAGGCTGAGT ATCGAGACGC TTATAGAGAG
    TACATTGCTC AGATGTCTCA GTTAGAAGGG GGCACAGGTT CCACAACCAT GAGTGGCAGG
    TCTTCTCCAC ACAGCACGTA TTATATGGGT CAGAGTTCAT CCGGGGGCTC CATTCACTCT
    AGTCTAGAAC CCGAGAAAGG GAAGGATAGT GAACCAAAGC AAGATGATAC ACGGAAGTCA
    TTTCTGATGA AGAGGGGAGA TGTTATAGAT TATTCCTCAT CAGGAGTTTC CACCAATGAT
    GCCTCACCCC TGGATCCTAT TACCGAGGAA GATGAAAAAT CTGATCAGTC AGGCAGTAAG
    CTTCTCCCAG GCAAGAAGTC CTCAGAAAGG TCAAGTCTCT TCCAGACAGA CCTGAAGCTG
    AAGGGAGGTG GACTGCGCTA TCAGAAACTC CCGAGTGACG AAGACGAGTC TGGGACAGAA
    GAGTCAGACA ACACTCCACT GCTCAAAGAC GACAAAGACA AGAAAGCTGA AGGGAAAGCA
    GAGAGAGCAT TGAAGTCTCC AGAGCACAGT GCAGAGCCCA TTCGAACCTT CATCAAGGCC
    AAAGAGTACT TGTCAGATGC CCTCCTTGAC AAAAAGGACT CCTCTGACTC AGGAGTGAGG
    TCCAATGAGA GCTCGCCCAA CCACTCCCTT CACAACGAGG CGGCAGATGA CTCCCAGCTT
    GAAAAGGCCA ACCTCATAGA GCTCGAAGAT GACCCCCACA GTGGAAGACG GGGAGTCCCG
    CACAGCCTGA GTGGCTTGCC AGACCCCCTC GTAGCCCGGA TGTCCATTTG CTCCGAGGAC
    AAGAAGAGCC CTTCTGAATG CAGCTTGATA GCCAGCAGCC CCGAGGAAAA CTGGCCAGCA
    TGCCACAAAT CCTACAACCT GAATCGCACC CCCAGCACCG TGACCCTGAA CAACAACAGT
    GCCCCGGCTA ACAGAGCCAA CCAGAATTCC GATGAGACGG AGGGGCTGAG GGAGACGCCT
    CAAGTCCTCC TGAGGTCAGG CTCCAGTTCC CACTCCACCG CTCTGCAGAA TGAGAACCTG
    AAGAGCATGG CACACAAGCG AGGCCAGCGT TCCAGCTACA CGAGGCTCTC AAAGGACTCC
    TCAGAGCTCC ACGCAGTAGC CTCCTCCGAT AGTACAGGTT TTGGAGAAGA AAGGGAGAGC
    ATTCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320926.1
    CDS207..5555
    Translation

    Target ORF information:

    RefSeq Version XM_015465440.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465440.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GCCGTGTGAC
    TCTGGGTTTA ACAAACAGAG ACAGGGATCA GGCCCAGCCT CAGGCACAAT ACCACTGAAT
    TCGATGAACG TGGATGCAGT TTGTGAGAAA CTGAAACAAA TTGAAGGGCT GGACCAGAGC
    ATGCTGCCTC AGTACTGTGC AACCATCAAA AAGGCGAACA TAAATGGCCG TGTGTTGGCT
    CAGTGTAACA TTGATGAACT GAAGAAGGAA ATGAATATGA ATTTTGGAGA CTGGCATCTT
    TTTAGAAGCA CAATATTAGA AATGAGAAAT GCAGAAAACC AAGTGGTCCC TGAAGACCCA
    CGTTTACTGA GTGAAAACAG CAGCTCTGTT CCTCACGGTG AACCTGCTCG TCGCTCCGCA
    CACAGCGAGC TGCCCCACAC CGAGCTCTCC AGTCAGGCTC CCTACACCCT GAACTTCAGC
    TTTGAAGAGC TGAACACGCT CGGCCTGGAC GAAGGGGCTC CTCGCCACAG CAACCTGAGT
    TGGCAGTCAC AAACTCGCAG AACCCCAAGT CTTTCCAGTC TCACTTCCCA GGACTCCAGT
    ATTGAAATTT CAAAGCTTAC TGATAAGGTG CAGGCTGAGT ATCGAGACGC TTATAGAGAG
    TACATTGCTC AGATGTCTCA GTTAGAAGGG GGCACAGGTT CCACAACCAT GAGTGGCAGG
    TCTTCTCCAC ACAGCACGTA TTATATGGGT CAGAGTTCAT CCGGGGGCTC CATTCACTCT
    AGTCTAGAAC CCGAGAAAGG GAAGGATAGT GAACCAAAGC AAGATGATAC ACGGAAGTCA
    TTTCTGATGA AGAGGGGAGA TGTTATAGAT TATTCCTCAT CAGGAGTTTC CACCAATGAT
    GCCTCACCCC TGGATCCTAT TACCGAGGAA GATGAAAAAT CTGATCAGTC AGGCAGTAAG
    CTTCTCCCAG GCAAGAAGTC CTCAGAAAGG TCAAGTCTCT TCCAGACAGA CCTGAAGCTG
    AAGGGAGGTG GACTGCGCTA TCAGAAACTC CCGAGTGACG AAGACGAGTC TGGGACAGAA
    GAGTCAGACA ACACTCCACT GCTCAAAGAC GACAAAGACA AGAAAGCTGA AGGGAAAGCA
    GAGAGAGCAT TGAAGTCTCC AGAGCACAGT GCAGAGCCCA TTCGAACCTT CATCAAGGCC
    AAAGAGTACT TGTCAGATGC CCTCCTTGAC AAAAAGGACT CCTCTGACTC AGGAGTGAGG
    TCCAATGAGA GCTCGCCCAA CCACTCCCTT CACAACGAGG CGGCAGATGA CTCCCAGCTT
    GAAAAGGCCA ACCTCATAGA GCTCGAAGAT GACCCCCACA GTGGAAGACG GGGAGTCCCG
    CACAGCCTGA GTGGCTTGCC AGACCCCCTC GTAGCCCGGA TGTCCATTTG CTCCGAGGAC
    AAGAAGAGCC CTTCTGAATG CAGCTTGATA GCCAGCAGCC CCGAGGAAAA CTGGCCAGCA
    TGCCACAAAT CCTACAACCT GAATCGCACC CCCAGCACCG TGACCCTGAA CAACAACAGT
    GCCCCGGCTA ACAGAGCCAA CCAGAATTCC GATGAGACGG AGGGGCTGAG GGAGACGCCT
    CAAGTCCTCC TGAGGTCAGG CTCCAGTTCC CACTCCACCG CTCTGCAGAA TGAGAACCTG
    AAGAGCATGG CACACAAGCG AGGCCAGCGT TCCAGCTACA CGAGGCTCTC AAAGGACTCC
    TCAGAGCTCC ACGCAGTAGC CTCCTCCGAT AGTACAGGTT TTGGAGAAGA AAGGGAGAGC
    ATTCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24271
    Clone ID Related Accession (Same CDS sequence) XM_005213101.3 , XM_015465440.1 , XM_005213101.4 , XM_024999548.1
    Accession Version XM_005213101.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213101.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GCCGTGTGAC
    TCTGGGTTTA ACAAACAGAG ACAGGGATCA GGCCCAGCCT CAGGCACAAT ACCACTGAAT
    TCGATGAACG TGGATGCAGT TTGTGAGAAA CTGAAACAAA TTGAAGGGCT GGACCAGAGC
    ATGCTGCCTC AGTACTGTGC AACCATCAAA AAGGCGAACA TAAATGGCCG TGTGTTGGCT
    CAGTGTAACA TTGATGAACT GAAGAAGGAA ATGAATATGA ATTTTGGAGA CTGGCATCTT
    TTTAGAAGCA CAATATTAGA AATGAGAAAT GCAGAAAACC AAGTGGTCCC TGAAGACCCA
    CGTTTACTGA GTGAAAACAG CAGCTCTGTT CCTCACGGTG AACCTGCTCG TCGCTCCGCA
    CACAGCGAGC TGCCCCACAC CGAGCTCTCC AGTCAGGCTC CCTACACCCT GAACTTCAGC
    TTTGAAGAGC TGAACACGCT CGGCCTGGAC GAAGGGGCTC CTCGCCACAG CAACCTGAGT
    TGGCAGTCAC AAACTCGCAG AACCCCAAGT CTTTCCAGTC TCACTTCCCA GGACTCCAGT
    ATTGAAATTT CAAAGCTTAC TGATAAGGTG CAGGCTGAGT ATCGAGACGC TTATAGAGAG
    TACATTGCTC AGATGTCTCA GTTAGAAGGG GGCACAGGTT CCACAACCAT GAGTGGCAGG
    TCTTCTCCAC ACAGCACGTA TTATATGGGT CAGAGTTCAT CCGGGGGCTC CATTCACTCT
    AGTCTAGAAC CCGAGAAAGG GAAGGATAGT GAACCAAAGC AAGATGATAC ACGGAAGTCA
    TTTCTGATGA AGAGGGGAGA TGTTATAGAT TATTCCTCAT CAGGAGTTTC CACCAATGAT
    GCCTCACCCC TGGATCCTAT TACCGAGGAA GATGAAAAAT CTGATCAGTC AGGCAGTAAG
    CTTCTCCCAG GCAAGAAGTC CTCAGAAAGG TCAAGTCTCT TCCAGACAGA CCTGAAGCTG
    AAGGGAGGTG GACTGCGCTA TCAGAAACTC CCGAGTGACG AAGACGAGTC TGGGACAGAA
    GAGTCAGACA ACACTCCACT GCTCAAAGAC GACAAAGACA AGAAAGCTGA AGGGAAAGCA
    GAGAGAGCAT TGAAGTCTCC AGAGCACAGT GCAGAGCCCA TTCGAACCTT CATCAAGGCC
    AAAGAGTACT TGTCAGATGC CCTCCTTGAC AAAAAGGACT CCTCTGACTC AGGAGTGAGG
    TCCAATGAGA GCTCGCCCAA CCACTCCCTT CACAACGAGG CGGCAGATGA CTCCCAGCTT
    GAAAAGGCCA ACCTCATAGA GCTCGAAGAT GACCCCCACA GTGGAAGACG GGGAGTCCCG
    CACAGCCTGA GTGGCTTGCC AGACCCCCTC GTAGCCCGGA TGTCCATTTG CTCCGAGGAC
    AAGAAGAGCC CTTCTGAATG CAGCTTGATA GCCAGCAGCC CCGAGGAAAA CTGGCCAGCA
    TGCCACAAAT CCTACAACCT GAATCGCACC CCCAGCACCG TGACCCTGAA CAACAACAGT
    GCCCCGGCTA ACAGAGCCAA CCAGAATTCC GATGAGACGG AGGGGCTGAG GGAGACGCCT
    CAAGTCCTCC TGAGGTCAGG CTCCAGTTCC CACTCCACCG CTCTGCAGAA TGAGAACCTG
    AAGAGCATGG CACACAAGCG AGGCCAGCGT TCCAGCTACA CGAGGCTCTC AAAGGACTCC
    TCAGAGCTCC ACGCAGTAGC CTCCTCCGAT AGTACAGGTT TTGGAGAAGA AAGGGAGAGC
    ATTCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213158.1
    CDS213..5561
    Translation

    Target ORF information:

    RefSeq Version XM_005213101.4
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213101.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GCCGTGTGAC
    TCTGGGTTTA ACAAACAGAG ACAGGGATCA GGCCCAGCCT CAGGCACAAT ACCACTGAAT
    TCGATGAACG TGGATGCAGT TTGTGAGAAA CTGAAACAAA TTGAAGGGCT GGACCAGAGC
    ATGCTGCCTC AGTACTGTGC AACCATCAAA AAGGCGAACA TAAATGGCCG TGTGTTGGCT
    CAGTGTAACA TTGATGAACT GAAGAAGGAA ATGAATATGA ATTTTGGAGA CTGGCATCTT
    TTTAGAAGCA CAATATTAGA AATGAGAAAT GCAGAAAACC AAGTGGTCCC TGAAGACCCA
    CGTTTACTGA GTGAAAACAG CAGCTCTGTT CCTCACGGTG AACCTGCTCG TCGCTCCGCA
    CACAGCGAGC TGCCCCACAC CGAGCTCTCC AGTCAGGCTC CCTACACCCT GAACTTCAGC
    TTTGAAGAGC TGAACACGCT CGGCCTGGAC GAAGGGGCTC CTCGCCACAG CAACCTGAGT
    TGGCAGTCAC AAACTCGCAG AACCCCAAGT CTTTCCAGTC TCACTTCCCA GGACTCCAGT
    ATTGAAATTT CAAAGCTTAC TGATAAGGTG CAGGCTGAGT ATCGAGACGC TTATAGAGAG
    TACATTGCTC AGATGTCTCA GTTAGAAGGG GGCACAGGTT CCACAACCAT GAGTGGCAGG
    TCTTCTCCAC ACAGCACGTA TTATATGGGT CAGAGTTCAT CCGGGGGCTC CATTCACTCT
    AGTCTAGAAC CCGAGAAAGG GAAGGATAGT GAACCAAAGC AAGATGATAC ACGGAAGTCA
    TTTCTGATGA AGAGGGGAGA TGTTATAGAT TATTCCTCAT CAGGAGTTTC CACCAATGAT
    GCCTCACCCC TGGATCCTAT TACCGAGGAA GATGAAAAAT CTGATCAGTC AGGCAGTAAG
    CTTCTCCCAG GCAAGAAGTC CTCAGAAAGG TCAAGTCTCT TCCAGACAGA CCTGAAGCTG
    AAGGGAGGTG GACTGCGCTA TCAGAAACTC CCGAGTGACG AAGACGAGTC TGGGACAGAA
    GAGTCAGACA ACACTCCACT GCTCAAAGAC GACAAAGACA AGAAAGCTGA AGGGAAAGCA
    GAGAGAGCAT TGAAGTCTCC AGAGCACAGT GCAGAGCCCA TTCGAACCTT CATCAAGGCC
    AAAGAGTACT TGTCAGATGC CCTCCTTGAC AAAAAGGACT CCTCTGACTC AGGAGTGAGG
    TCCAATGAGA GCTCGCCCAA CCACTCCCTT CACAACGAGG CGGCAGATGA CTCCCAGCTT
    GAAAAGGCCA ACCTCATAGA GCTCGAAGAT GACCCCCACA GTGGAAGACG GGGAGTCCCG
    CACAGCCTGA GTGGCTTGCC AGACCCCCTC GTAGCCCGGA TGTCCATTTG CTCCGAGGAC
    AAGAAGAGCC CTTCTGAATG CAGCTTGATA GCCAGCAGCC CCGAGGAAAA CTGGCCAGCA
    TGCCACAAAT CCTACAACCT GAATCGCACC CCCAGCACCG TGACCCTGAA CAACAACAGT
    GCCCCGGCTA ACAGAGCCAA CCAGAATTCC GATGAGACGG AGGGGCTGAG GGAGACGCCT
    CAAGTCCTCC TGAGGTCAGG CTCCAGTTCC CACTCCACCG CTCTGCAGAA TGAGAACCTG
    AAGAGCATGG CACACAAGCG AGGCCAGCGT TCCAGCTACA CGAGGCTCTC AAAGGACTCC
    TCAGAGCTCC ACGCAGTAGC CTCCTCCGAT AGTACAGGTT TTGGAGAAGA AAGGGAGAGC
    ATTCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24271
    Clone ID Related Accession (Same CDS sequence) XM_005213101.3 , XM_015465440.1 , XM_005213101.4 , XM_024999548.1
    Accession Version XM_024999548.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GCCGTGTGAC
    TCTGGGTTTA ACAAACAGAG ACAGGGATCA GGCCCAGCCT CAGGCACAAT ACCACTGAAT
    TCGATGAACG TGGATGCAGT TTGTGAGAAA CTGAAACAAA TTGAAGGGCT GGACCAGAGC
    ATGCTGCCTC AGTACTGTGC AACCATCAAA AAGGCGAACA TAAATGGCCG TGTGTTGGCT
    CAGTGTAACA TTGATGAACT GAAGAAGGAA ATGAATATGA ATTTTGGAGA CTGGCATCTT
    TTTAGAAGCA CAATATTAGA AATGAGAAAT GCAGAAAACC AAGTGGTCCC TGAAGACCCA
    CGTTTACTGA GTGAAAACAG CAGCTCTGTT CCTCACGGTG AACCTGCTCG TCGCTCCGCA
    CACAGCGAGC TGCCCCACAC CGAGCTCTCC AGTCAGGCTC CCTACACCCT GAACTTCAGC
    TTTGAAGAGC TGAACACGCT CGGCCTGGAC GAAGGGGCTC CTCGCCACAG CAACCTGAGT
    TGGCAGTCAC AAACTCGCAG AACCCCAAGT CTTTCCAGTC TCACTTCCCA GGACTCCAGT
    ATTGAAATTT CAAAGCTTAC TGATAAGGTG CAGGCTGAGT ATCGAGACGC TTATAGAGAG
    TACATTGCTC AGATGTCTCA GTTAGAAGGG GGCACAGGTT CCACAACCAT GAGTGGCAGG
    TCTTCTCCAC ACAGCACGTA TTATATGGGT CAGAGTTCAT CCGGGGGCTC CATTCACTCT
    AGTCTAGAAC CCGAGAAAGG GAAGGATAGT GAACCAAAGC AAGATGATAC ACGGAAGTCA
    TTTCTGATGA AGAGGGGAGA TGTTATAGAT TATTCCTCAT CAGGAGTTTC CACCAATGAT
    GCCTCACCCC TGGATCCTAT TACCGAGGAA GATGAAAAAT CTGATCAGTC AGGCAGTAAG
    CTTCTCCCAG GCAAGAAGTC CTCAGAAAGG TCAAGTCTCT TCCAGACAGA CCTGAAGCTG
    AAGGGAGGTG GACTGCGCTA TCAGAAACTC CCGAGTGACG AAGACGAGTC TGGGACAGAA
    GAGTCAGACA ACACTCCACT GCTCAAAGAC GACAAAGACA AGAAAGCTGA AGGGAAAGCA
    GAGAGAGCAT TGAAGTCTCC AGAGCACAGT GCAGAGCCCA TTCGAACCTT CATCAAGGCC
    AAAGAGTACT TGTCAGATGC CCTCCTTGAC AAAAAGGACT CCTCTGACTC AGGAGTGAGG
    TCCAATGAGA GCTCGCCCAA CCACTCCCTT CACAACGAGG CGGCAGATGA CTCCCAGCTT
    GAAAAGGCCA ACCTCATAGA GCTCGAAGAT GACCCCCACA GTGGAAGACG GGGAGTCCCG
    CACAGCCTGA GTGGCTTGCC AGACCCCCTC GTAGCCCGGA TGTCCATTTG CTCCGAGGAC
    AAGAAGAGCC CTTCTGAATG CAGCTTGATA GCCAGCAGCC CCGAGGAAAA CTGGCCAGCA
    TGCCACAAAT CCTACAACCT GAATCGCACC CCCAGCACCG TGACCCTGAA CAACAACAGT
    GCCCCGGCTA ACAGAGCCAA CCAGAATTCC GATGAGACGG AGGGGCTGAG GGAGACGCCT
    CAAGTCCTCC TGAGGTCAGG CTCCAGTTCC CACTCCACCG CTCTGCAGAA TGAGAACCTG
    AAGAGCATGG CACACAAGCG AGGCCAGCGT TCCAGCTACA CGAGGCTCTC AAAGGACTCC
    TCAGAGCTCC ACGCAGTAGC CTCCTCCGAT AGTACAGGTT TTGGAGAAGA AAGGGAGAGC
    ATTCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855316.1
    CDS309..5657
    Translation

    Target ORF information:

    RefSeq Version XM_024999548.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GCCGTGTGAC
    TCTGGGTTTA ACAAACAGAG ACAGGGATCA GGCCCAGCCT CAGGCACAAT ACCACTGAAT
    TCGATGAACG TGGATGCAGT TTGTGAGAAA CTGAAACAAA TTGAAGGGCT GGACCAGAGC
    ATGCTGCCTC AGTACTGTGC AACCATCAAA AAGGCGAACA TAAATGGCCG TGTGTTGGCT
    CAGTGTAACA TTGATGAACT GAAGAAGGAA ATGAATATGA ATTTTGGAGA CTGGCATCTT
    TTTAGAAGCA CAATATTAGA AATGAGAAAT GCAGAAAACC AAGTGGTCCC TGAAGACCCA
    CGTTTACTGA GTGAAAACAG CAGCTCTGTT CCTCACGGTG AACCTGCTCG TCGCTCCGCA
    CACAGCGAGC TGCCCCACAC CGAGCTCTCC AGTCAGGCTC CCTACACCCT GAACTTCAGC
    TTTGAAGAGC TGAACACGCT CGGCCTGGAC GAAGGGGCTC CTCGCCACAG CAACCTGAGT
    TGGCAGTCAC AAACTCGCAG AACCCCAAGT CTTTCCAGTC TCACTTCCCA GGACTCCAGT
    ATTGAAATTT CAAAGCTTAC TGATAAGGTG CAGGCTGAGT ATCGAGACGC TTATAGAGAG
    TACATTGCTC AGATGTCTCA GTTAGAAGGG GGCACAGGTT CCACAACCAT GAGTGGCAGG
    TCTTCTCCAC ACAGCACGTA TTATATGGGT CAGAGTTCAT CCGGGGGCTC CATTCACTCT
    AGTCTAGAAC CCGAGAAAGG GAAGGATAGT GAACCAAAGC AAGATGATAC ACGGAAGTCA
    TTTCTGATGA AGAGGGGAGA TGTTATAGAT TATTCCTCAT CAGGAGTTTC CACCAATGAT
    GCCTCACCCC TGGATCCTAT TACCGAGGAA GATGAAAAAT CTGATCAGTC AGGCAGTAAG
    CTTCTCCCAG GCAAGAAGTC CTCAGAAAGG TCAAGTCTCT TCCAGACAGA CCTGAAGCTG
    AAGGGAGGTG GACTGCGCTA TCAGAAACTC CCGAGTGACG AAGACGAGTC TGGGACAGAA
    GAGTCAGACA ACACTCCACT GCTCAAAGAC GACAAAGACA AGAAAGCTGA AGGGAAAGCA
    GAGAGAGCAT TGAAGTCTCC AGAGCACAGT GCAGAGCCCA TTCGAACCTT CATCAAGGCC
    AAAGAGTACT TGTCAGATGC CCTCCTTGAC AAAAAGGACT CCTCTGACTC AGGAGTGAGG
    TCCAATGAGA GCTCGCCCAA CCACTCCCTT CACAACGAGG CGGCAGATGA CTCCCAGCTT
    GAAAAGGCCA ACCTCATAGA GCTCGAAGAT GACCCCCACA GTGGAAGACG GGGAGTCCCG
    CACAGCCTGA GTGGCTTGCC AGACCCCCTC GTAGCCCGGA TGTCCATTTG CTCCGAGGAC
    AAGAAGAGCC CTTCTGAATG CAGCTTGATA GCCAGCAGCC CCGAGGAAAA CTGGCCAGCA
    TGCCACAAAT CCTACAACCT GAATCGCACC CCCAGCACCG TGACCCTGAA CAACAACAGT
    GCCCCGGCTA ACAGAGCCAA CCAGAATTCC GATGAGACGG AGGGGCTGAG GGAGACGCCT
    CAAGTCCTCC TGAGGTCAGG CTCCAGTTCC CACTCCACCG CTCTGCAGAA TGAGAACCTG
    AAGAGCATGG CACACAAGCG AGGCCAGCGT TCCAGCTACA CGAGGCTCTC AAAGGACTCC
    TCAGAGCTCC ACGCAGTAGC CTCCTCCGAT AGTACAGGTT TTGGAGAAGA AAGGGAGAGC
    ATTCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24273
    Clone ID Related Accession (Same CDS sequence) XM_015465441.1 , XM_005213103.4 , XM_005213103.3
    Accession Version XM_015465441.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGGATCAGGC
    CCAGCCTCAG GCACAATACC ACTGAATTCG ATGAACGTGG ATGCAGTTTG TGAGAAACTG
    AAACAAATTG AAGGGCTGGA CCAGAGCATG CTGCCTCAGT ACTGTGCAAC CATCAAAAAG
    GCGAACATAA ATGGCCGTGT GTTGGCTCAG TGTAACATTG ATGAACTGAA GAAGGAAATG
    AATATGAATT TTGGAGACTG GCATCTTTTT AGAAGCACAA TATTAGAAAT GAGAAATGCA
    GAAAACCAAG TGGTCCCTGA AGACCCACGT TTACTGAGTG AAAACAGCAG CTCTGTTCCT
    CACGGTGAAC CTGCTCGTCG CTCCGCACAC AGCGAGCTGC CCCACACCGA GCTCTCCAGT
    CAGGCTCCCT ACACCCTGAA CTTCAGCTTT GAAGAGCTGA ACACGCTCGG CCTGGACGAA
    GGGGCTCCTC GCCACAGCAA CCTGAGTTGG CAGTCACAAA CTCGCAGAAC CCCAAGTCTT
    TCCAGTCTCA CTTCCCAGGA CTCCAGTATT GAAATTTCAA AGCTTACTGA TAAGGTGCAG
    GCTGAGTATC GAGACGCTTA TAGAGAGTAC ATTGCTCAGA TGTCTCAGTT AGAAGGGGGC
    ACAGGTTCCA CAACCATGAG TGGCAGGTCT TCTCCACACA GCACGTATTA TATGGGTCAG
    AGTTCATCCG GGGGCTCCAT TCACTCTAGT CTAGAACCCG AGAAAGGGAA GGATAGTGAA
    CCAAAGCAAG ATGATACACG GAAGTCATTT CTGATGAAGA GGGGAGATGT TATAGATTAT
    TCCTCATCAG GAGTTTCCAC CAATGATGCC TCACCCCTGG ATCCTATTAC CGAGGAAGAT
    GAAAAATCTG ATCAGTCAGG CAGTAAGCTT CTCCCAGGCA AGAAGTCCTC AGAAAGGTCA
    AGTCTCTTCC AGACAGACCT GAAGCTGAAG GGAGGTGGAC TGCGCTATCA GAAACTCCCG
    AGTGACGAAG ACGAGTCTGG GACAGAAGAG TCAGACAACA CTCCACTGCT CAAAGACGAC
    AAAGACAAGA AAGCTGAAGG GAAAGCAGAG AGAGCATTGA AGTCTCCAGA GCACAGTGCA
    GAGCCCATTC GAACCTTCAT CAAGGCCAAA GAGTACTTGT CAGATGCCCT CCTTGACAAA
    AAGGACTCCT CTGACTCAGG AGTGAGGTCC AATGAGAGCT CGCCCAACCA CTCCCTTCAC
    AACGAGGCGG CAGATGACTC CCAGCTTGAA AAGGCCAACC TCATAGAGCT CGAAGATGAC
    CCCCACAGTG GAAGACGGGG AGTCCCGCAC AGCCTGAGTG GCTTGCCAGA CCCCCTCGTA
    GCCCGGATGT CCATTTGCTC CGAGGACAAG AAGAGCCCTT CTGAATGCAG CTTGATAGCC
    AGCAGCCCCG AGGAAAACTG GCCAGCATGC CACAAATCCT ACAACCTGAA TCGCACCCCC
    AGCACCGTGA CCCTGAACAA CAACAGTGCC CCGGCTAACA GAGCCAACCA GAATTCCGAT
    GAGACGGAGG GGCTGAGGGA GACGCCTCAA GTCCTCCTGA GGTCAGGCTC CAGTTCCCAC
    TCCACCGCTC TGCAGAATGA GAACCTGAAG AGCATGGCAC ACAAGCGAGG CCAGCGTTCC
    AGCTACACGA GGCTCTCAAA GGACTCCTCA GAGCTCCACG CAGTAGCCTC CTCCGATAGT
    ACAGGTTTTG GAGAAGAAAG GGAGAGCATT CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320927.1
    CDS207..5522
    Translation

    Target ORF information:

    RefSeq Version XM_015465441.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465441.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGGATCAGGC
    CCAGCCTCAG GCACAATACC ACTGAATTCG ATGAACGTGG ATGCAGTTTG TGAGAAACTG
    AAACAAATTG AAGGGCTGGA CCAGAGCATG CTGCCTCAGT ACTGTGCAAC CATCAAAAAG
    GCGAACATAA ATGGCCGTGT GTTGGCTCAG TGTAACATTG ATGAACTGAA GAAGGAAATG
    AATATGAATT TTGGAGACTG GCATCTTTTT AGAAGCACAA TATTAGAAAT GAGAAATGCA
    GAAAACCAAG TGGTCCCTGA AGACCCACGT TTACTGAGTG AAAACAGCAG CTCTGTTCCT
    CACGGTGAAC CTGCTCGTCG CTCCGCACAC AGCGAGCTGC CCCACACCGA GCTCTCCAGT
    CAGGCTCCCT ACACCCTGAA CTTCAGCTTT GAAGAGCTGA ACACGCTCGG CCTGGACGAA
    GGGGCTCCTC GCCACAGCAA CCTGAGTTGG CAGTCACAAA CTCGCAGAAC CCCAAGTCTT
    TCCAGTCTCA CTTCCCAGGA CTCCAGTATT GAAATTTCAA AGCTTACTGA TAAGGTGCAG
    GCTGAGTATC GAGACGCTTA TAGAGAGTAC ATTGCTCAGA TGTCTCAGTT AGAAGGGGGC
    ACAGGTTCCA CAACCATGAG TGGCAGGTCT TCTCCACACA GCACGTATTA TATGGGTCAG
    AGTTCATCCG GGGGCTCCAT TCACTCTAGT CTAGAACCCG AGAAAGGGAA GGATAGTGAA
    CCAAAGCAAG ATGATACACG GAAGTCATTT CTGATGAAGA GGGGAGATGT TATAGATTAT
    TCCTCATCAG GAGTTTCCAC CAATGATGCC TCACCCCTGG ATCCTATTAC CGAGGAAGAT
    GAAAAATCTG ATCAGTCAGG CAGTAAGCTT CTCCCAGGCA AGAAGTCCTC AGAAAGGTCA
    AGTCTCTTCC AGACAGACCT GAAGCTGAAG GGAGGTGGAC TGCGCTATCA GAAACTCCCG
    AGTGACGAAG ACGAGTCTGG GACAGAAGAG TCAGACAACA CTCCACTGCT CAAAGACGAC
    AAAGACAAGA AAGCTGAAGG GAAAGCAGAG AGAGCATTGA AGTCTCCAGA GCACAGTGCA
    GAGCCCATTC GAACCTTCAT CAAGGCCAAA GAGTACTTGT CAGATGCCCT CCTTGACAAA
    AAGGACTCCT CTGACTCAGG AGTGAGGTCC AATGAGAGCT CGCCCAACCA CTCCCTTCAC
    AACGAGGCGG CAGATGACTC CCAGCTTGAA AAGGCCAACC TCATAGAGCT CGAAGATGAC
    CCCCACAGTG GAAGACGGGG AGTCCCGCAC AGCCTGAGTG GCTTGCCAGA CCCCCTCGTA
    GCCCGGATGT CCATTTGCTC CGAGGACAAG AAGAGCCCTT CTGAATGCAG CTTGATAGCC
    AGCAGCCCCG AGGAAAACTG GCCAGCATGC CACAAATCCT ACAACCTGAA TCGCACCCCC
    AGCACCGTGA CCCTGAACAA CAACAGTGCC CCGGCTAACA GAGCCAACCA GAATTCCGAT
    GAGACGGAGG GGCTGAGGGA GACGCCTCAA GTCCTCCTGA GGTCAGGCTC CAGTTCCCAC
    TCCACCGCTC TGCAGAATGA GAACCTGAAG AGCATGGCAC ACAAGCGAGG CCAGCGTTCC
    AGCTACACGA GGCTCTCAAA GGACTCCTCA GAGCTCCACG CAGTAGCCTC CTCCGATAGT
    ACAGGTTTTG GAGAAGAAAG GGAGAGCATT CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24273
    Clone ID Related Accession (Same CDS sequence) XM_015465441.1 , XM_005213103.4 , XM_005213103.3
    Accession Version XM_005213103.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213103.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGGATCAGGC
    CCAGCCTCAG GCACAATACC ACTGAATTCG ATGAACGTGG ATGCAGTTTG TGAGAAACTG
    AAACAAATTG AAGGGCTGGA CCAGAGCATG CTGCCTCAGT ACTGTGCAAC CATCAAAAAG
    GCGAACATAA ATGGCCGTGT GTTGGCTCAG TGTAACATTG ATGAACTGAA GAAGGAAATG
    AATATGAATT TTGGAGACTG GCATCTTTTT AGAAGCACAA TATTAGAAAT GAGAAATGCA
    GAAAACCAAG TGGTCCCTGA AGACCCACGT TTACTGAGTG AAAACAGCAG CTCTGTTCCT
    CACGGTGAAC CTGCTCGTCG CTCCGCACAC AGCGAGCTGC CCCACACCGA GCTCTCCAGT
    CAGGCTCCCT ACACCCTGAA CTTCAGCTTT GAAGAGCTGA ACACGCTCGG CCTGGACGAA
    GGGGCTCCTC GCCACAGCAA CCTGAGTTGG CAGTCACAAA CTCGCAGAAC CCCAAGTCTT
    TCCAGTCTCA CTTCCCAGGA CTCCAGTATT GAAATTTCAA AGCTTACTGA TAAGGTGCAG
    GCTGAGTATC GAGACGCTTA TAGAGAGTAC ATTGCTCAGA TGTCTCAGTT AGAAGGGGGC
    ACAGGTTCCA CAACCATGAG TGGCAGGTCT TCTCCACACA GCACGTATTA TATGGGTCAG
    AGTTCATCCG GGGGCTCCAT TCACTCTAGT CTAGAACCCG AGAAAGGGAA GGATAGTGAA
    CCAAAGCAAG ATGATACACG GAAGTCATTT CTGATGAAGA GGGGAGATGT TATAGATTAT
    TCCTCATCAG GAGTTTCCAC CAATGATGCC TCACCCCTGG ATCCTATTAC CGAGGAAGAT
    GAAAAATCTG ATCAGTCAGG CAGTAAGCTT CTCCCAGGCA AGAAGTCCTC AGAAAGGTCA
    AGTCTCTTCC AGACAGACCT GAAGCTGAAG GGAGGTGGAC TGCGCTATCA GAAACTCCCG
    AGTGACGAAG ACGAGTCTGG GACAGAAGAG TCAGACAACA CTCCACTGCT CAAAGACGAC
    AAAGACAAGA AAGCTGAAGG GAAAGCAGAG AGAGCATTGA AGTCTCCAGA GCACAGTGCA
    GAGCCCATTC GAACCTTCAT CAAGGCCAAA GAGTACTTGT CAGATGCCCT CCTTGACAAA
    AAGGACTCCT CTGACTCAGG AGTGAGGTCC AATGAGAGCT CGCCCAACCA CTCCCTTCAC
    AACGAGGCGG CAGATGACTC CCAGCTTGAA AAGGCCAACC TCATAGAGCT CGAAGATGAC
    CCCCACAGTG GAAGACGGGG AGTCCCGCAC AGCCTGAGTG GCTTGCCAGA CCCCCTCGTA
    GCCCGGATGT CCATTTGCTC CGAGGACAAG AAGAGCCCTT CTGAATGCAG CTTGATAGCC
    AGCAGCCCCG AGGAAAACTG GCCAGCATGC CACAAATCCT ACAACCTGAA TCGCACCCCC
    AGCACCGTGA CCCTGAACAA CAACAGTGCC CCGGCTAACA GAGCCAACCA GAATTCCGAT
    GAGACGGAGG GGCTGAGGGA GACGCCTCAA GTCCTCCTGA GGTCAGGCTC CAGTTCCCAC
    TCCACCGCTC TGCAGAATGA GAACCTGAAG AGCATGGCAC ACAAGCGAGG CCAGCGTTCC
    AGCTACACGA GGCTCTCAAA GGACTCCTCA GAGCTCCACG CAGTAGCCTC CTCCGATAGT
    ACAGGTTTTG GAGAAGAAAG GGAGAGCATT CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213160.1
    CDS213..5528
    Translation

    Target ORF information:

    RefSeq Version XM_005213103.4
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213103.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGGATCAGGC
    CCAGCCTCAG GCACAATACC ACTGAATTCG ATGAACGTGG ATGCAGTTTG TGAGAAACTG
    AAACAAATTG AAGGGCTGGA CCAGAGCATG CTGCCTCAGT ACTGTGCAAC CATCAAAAAG
    GCGAACATAA ATGGCCGTGT GTTGGCTCAG TGTAACATTG ATGAACTGAA GAAGGAAATG
    AATATGAATT TTGGAGACTG GCATCTTTTT AGAAGCACAA TATTAGAAAT GAGAAATGCA
    GAAAACCAAG TGGTCCCTGA AGACCCACGT TTACTGAGTG AAAACAGCAG CTCTGTTCCT
    CACGGTGAAC CTGCTCGTCG CTCCGCACAC AGCGAGCTGC CCCACACCGA GCTCTCCAGT
    CAGGCTCCCT ACACCCTGAA CTTCAGCTTT GAAGAGCTGA ACACGCTCGG CCTGGACGAA
    GGGGCTCCTC GCCACAGCAA CCTGAGTTGG CAGTCACAAA CTCGCAGAAC CCCAAGTCTT
    TCCAGTCTCA CTTCCCAGGA CTCCAGTATT GAAATTTCAA AGCTTACTGA TAAGGTGCAG
    GCTGAGTATC GAGACGCTTA TAGAGAGTAC ATTGCTCAGA TGTCTCAGTT AGAAGGGGGC
    ACAGGTTCCA CAACCATGAG TGGCAGGTCT TCTCCACACA GCACGTATTA TATGGGTCAG
    AGTTCATCCG GGGGCTCCAT TCACTCTAGT CTAGAACCCG AGAAAGGGAA GGATAGTGAA
    CCAAAGCAAG ATGATACACG GAAGTCATTT CTGATGAAGA GGGGAGATGT TATAGATTAT
    TCCTCATCAG GAGTTTCCAC CAATGATGCC TCACCCCTGG ATCCTATTAC CGAGGAAGAT
    GAAAAATCTG ATCAGTCAGG CAGTAAGCTT CTCCCAGGCA AGAAGTCCTC AGAAAGGTCA
    AGTCTCTTCC AGACAGACCT GAAGCTGAAG GGAGGTGGAC TGCGCTATCA GAAACTCCCG
    AGTGACGAAG ACGAGTCTGG GACAGAAGAG TCAGACAACA CTCCACTGCT CAAAGACGAC
    AAAGACAAGA AAGCTGAAGG GAAAGCAGAG AGAGCATTGA AGTCTCCAGA GCACAGTGCA
    GAGCCCATTC GAACCTTCAT CAAGGCCAAA GAGTACTTGT CAGATGCCCT CCTTGACAAA
    AAGGACTCCT CTGACTCAGG AGTGAGGTCC AATGAGAGCT CGCCCAACCA CTCCCTTCAC
    AACGAGGCGG CAGATGACTC CCAGCTTGAA AAGGCCAACC TCATAGAGCT CGAAGATGAC
    CCCCACAGTG GAAGACGGGG AGTCCCGCAC AGCCTGAGTG GCTTGCCAGA CCCCCTCGTA
    GCCCGGATGT CCATTTGCTC CGAGGACAAG AAGAGCCCTT CTGAATGCAG CTTGATAGCC
    AGCAGCCCCG AGGAAAACTG GCCAGCATGC CACAAATCCT ACAACCTGAA TCGCACCCCC
    AGCACCGTGA CCCTGAACAA CAACAGTGCC CCGGCTAACA GAGCCAACCA GAATTCCGAT
    GAGACGGAGG GGCTGAGGGA GACGCCTCAA GTCCTCCTGA GGTCAGGCTC CAGTTCCCAC
    TCCACCGCTC TGCAGAATGA GAACCTGAAG AGCATGGCAC ACAAGCGAGG CCAGCGTTCC
    AGCTACACGA GGCTCTCAAA GGACTCCTCA GAGCTCCACG CAGTAGCCTC CTCCGATAGT
    ACAGGTTTTG GAGAAGAAAG GGAGAGCATT CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24273
    Clone ID Related Accession (Same CDS sequence) XM_015465441.1 , XM_005213103.4 , XM_005213103.3
    Accession Version XM_005213103.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213103.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGGATCAGGC
    CCAGCCTCAG GCACAATACC ACTGAATTCG ATGAACGTGG ATGCAGTTTG TGAGAAACTG
    AAACAAATTG AAGGGCTGGA CCAGAGCATG CTGCCTCAGT ACTGTGCAAC CATCAAAAAG
    GCGAACATAA ATGGCCGTGT GTTGGCTCAG TGTAACATTG ATGAACTGAA GAAGGAAATG
    AATATGAATT TTGGAGACTG GCATCTTTTT AGAAGCACAA TATTAGAAAT GAGAAATGCA
    GAAAACCAAG TGGTCCCTGA AGACCCACGT TTACTGAGTG AAAACAGCAG CTCTGTTCCT
    CACGGTGAAC CTGCTCGTCG CTCCGCACAC AGCGAGCTGC CCCACACCGA GCTCTCCAGT
    CAGGCTCCCT ACACCCTGAA CTTCAGCTTT GAAGAGCTGA ACACGCTCGG CCTGGACGAA
    GGGGCTCCTC GCCACAGCAA CCTGAGTTGG CAGTCACAAA CTCGCAGAAC CCCAAGTCTT
    TCCAGTCTCA CTTCCCAGGA CTCCAGTATT GAAATTTCAA AGCTTACTGA TAAGGTGCAG
    GCTGAGTATC GAGACGCTTA TAGAGAGTAC ATTGCTCAGA TGTCTCAGTT AGAAGGGGGC
    ACAGGTTCCA CAACCATGAG TGGCAGGTCT TCTCCACACA GCACGTATTA TATGGGTCAG
    AGTTCATCCG GGGGCTCCAT TCACTCTAGT CTAGAACCCG AGAAAGGGAA GGATAGTGAA
    CCAAAGCAAG ATGATACACG GAAGTCATTT CTGATGAAGA GGGGAGATGT TATAGATTAT
    TCCTCATCAG GAGTTTCCAC CAATGATGCC TCACCCCTGG ATCCTATTAC CGAGGAAGAT
    GAAAAATCTG ATCAGTCAGG CAGTAAGCTT CTCCCAGGCA AGAAGTCCTC AGAAAGGTCA
    AGTCTCTTCC AGACAGACCT GAAGCTGAAG GGAGGTGGAC TGCGCTATCA GAAACTCCCG
    AGTGACGAAG ACGAGTCTGG GACAGAAGAG TCAGACAACA CTCCACTGCT CAAAGACGAC
    AAAGACAAGA AAGCTGAAGG GAAAGCAGAG AGAGCATTGA AGTCTCCAGA GCACAGTGCA
    GAGCCCATTC GAACCTTCAT CAAGGCCAAA GAGTACTTGT CAGATGCCCT CCTTGACAAA
    AAGGACTCCT CTGACTCAGG AGTGAGGTCC AATGAGAGCT CGCCCAACCA CTCCCTTCAC
    AACGAGGCGG CAGATGACTC CCAGCTTGAA AAGGCCAACC TCATAGAGCT CGAAGATGAC
    CCCCACAGTG GAAGACGGGG AGTCCCGCAC AGCCTGAGTG GCTTGCCAGA CCCCCTCGTA
    GCCCGGATGT CCATTTGCTC CGAGGACAAG AAGAGCCCTT CTGAATGCAG CTTGATAGCC
    AGCAGCCCCG AGGAAAACTG GCCAGCATGC CACAAATCCT ACAACCTGAA TCGCACCCCC
    AGCACCGTGA CCCTGAACAA CAACAGTGCC CCGGCTAACA GAGCCAACCA GAATTCCGAT
    GAGACGGAGG GGCTGAGGGA GACGCCTCAA GTCCTCCTGA GGTCAGGCTC CAGTTCCCAC
    TCCACCGCTC TGCAGAATGA GAACCTGAAG AGCATGGCAC ACAAGCGAGG CCAGCGTTCC
    AGCTACACGA GGCTCTCAAA GGACTCCTCA GAGCTCCACG CAGTAGCCTC CTCCGATAGT
    ACAGGTTTTG GAGAAGAAAG GGAGAGCATT CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213160.1
    CDS207..5522
    Translation

    Target ORF information:

    RefSeq Version XM_005213103.3
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213103.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGGATCAGGC
    CCAGCCTCAG GCACAATACC ACTGAATTCG ATGAACGTGG ATGCAGTTTG TGAGAAACTG
    AAACAAATTG AAGGGCTGGA CCAGAGCATG CTGCCTCAGT ACTGTGCAAC CATCAAAAAG
    GCGAACATAA ATGGCCGTGT GTTGGCTCAG TGTAACATTG ATGAACTGAA GAAGGAAATG
    AATATGAATT TTGGAGACTG GCATCTTTTT AGAAGCACAA TATTAGAAAT GAGAAATGCA
    GAAAACCAAG TGGTCCCTGA AGACCCACGT TTACTGAGTG AAAACAGCAG CTCTGTTCCT
    CACGGTGAAC CTGCTCGTCG CTCCGCACAC AGCGAGCTGC CCCACACCGA GCTCTCCAGT
    CAGGCTCCCT ACACCCTGAA CTTCAGCTTT GAAGAGCTGA ACACGCTCGG CCTGGACGAA
    GGGGCTCCTC GCCACAGCAA CCTGAGTTGG CAGTCACAAA CTCGCAGAAC CCCAAGTCTT
    TCCAGTCTCA CTTCCCAGGA CTCCAGTATT GAAATTTCAA AGCTTACTGA TAAGGTGCAG
    GCTGAGTATC GAGACGCTTA TAGAGAGTAC ATTGCTCAGA TGTCTCAGTT AGAAGGGGGC
    ACAGGTTCCA CAACCATGAG TGGCAGGTCT TCTCCACACA GCACGTATTA TATGGGTCAG
    AGTTCATCCG GGGGCTCCAT TCACTCTAGT CTAGAACCCG AGAAAGGGAA GGATAGTGAA
    CCAAAGCAAG ATGATACACG GAAGTCATTT CTGATGAAGA GGGGAGATGT TATAGATTAT
    TCCTCATCAG GAGTTTCCAC CAATGATGCC TCACCCCTGG ATCCTATTAC CGAGGAAGAT
    GAAAAATCTG ATCAGTCAGG CAGTAAGCTT CTCCCAGGCA AGAAGTCCTC AGAAAGGTCA
    AGTCTCTTCC AGACAGACCT GAAGCTGAAG GGAGGTGGAC TGCGCTATCA GAAACTCCCG
    AGTGACGAAG ACGAGTCTGG GACAGAAGAG TCAGACAACA CTCCACTGCT CAAAGACGAC
    AAAGACAAGA AAGCTGAAGG GAAAGCAGAG AGAGCATTGA AGTCTCCAGA GCACAGTGCA
    GAGCCCATTC GAACCTTCAT CAAGGCCAAA GAGTACTTGT CAGATGCCCT CCTTGACAAA
    AAGGACTCCT CTGACTCAGG AGTGAGGTCC AATGAGAGCT CGCCCAACCA CTCCCTTCAC
    AACGAGGCGG CAGATGACTC CCAGCTTGAA AAGGCCAACC TCATAGAGCT CGAAGATGAC
    CCCCACAGTG GAAGACGGGG AGTCCCGCAC AGCCTGAGTG GCTTGCCAGA CCCCCTCGTA
    GCCCGGATGT CCATTTGCTC CGAGGACAAG AAGAGCCCTT CTGAATGCAG CTTGATAGCC
    AGCAGCCCCG AGGAAAACTG GCCAGCATGC CACAAATCCT ACAACCTGAA TCGCACCCCC
    AGCACCGTGA CCCTGAACAA CAACAGTGCC CCGGCTAACA GAGCCAACCA GAATTCCGAT
    GAGACGGAGG GGCTGAGGGA GACGCCTCAA GTCCTCCTGA GGTCAGGCTC CAGTTCCCAC
    TCCACCGCTC TGCAGAATGA GAACCTGAAG AGCATGGCAC ACAAGCGAGG CCAGCGTTCC
    AGCTACACGA GGCTCTCAAA GGACTCCTCA GAGCTCCACG CAGTAGCCTC CTCCGATAGT
    ACAGGTTTTG GAGAAGAAAG GGAGAGCATT CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24274
    Clone ID Related Accession (Same CDS sequence) XM_003586654.5 , XM_003586654.4 , XM_003582816.4
    Accession Version XM_003586654.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586654.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGAAGTTATC
    AAGGAGGATG CTGCTGAGGG CCTTTCTTCA CCCACAGACT CCTCGAGGGG ATCAGGCCCA
    GCCTCAGGCA CAATACCACT GAATTCGATG AACGTGGATG CAGTTTGTGA GAAACTGAAA
    CAAATTGAAG GGCTGGACCA GAGCATGCTG CCTCAGTACT GTGCAACCAT CAAAAAGGCG
    AACATAAATG GCCGTGTGTT GGCTCAGTGT AACATTGATG AACTGAAGAA GGAAATGAAT
    ATGAATTTTG GAGACTGGCA TCTTTTTAGA AGCACAATAT TAGAAATGAG AAATGCAGAA
    AACCAAGTGG TCCCTGAAGA CCCACGTTTA CTGAGTGAAA ACAGCAGCTC TGTTCCTCAC
    GGTGAACCTG CTCGTCGCTC CGCACACAGC GAGCTGCCCC ACACCGAGCT CTCCAGTCAG
    GCTCCCTACA CCCTGAACTT CAGCTTTGAA GAGCTGAACA CGCTCGGCCT GGACGAAGGG
    GCTCCTCGCC ACAGCAACCT GAGTTGGCAG TCACAAACTC GCAGAACCCC AAGTCTTTCC
    AGTCTCACTT CCCAGGACTC CAGTATTGAA ATTTCAAAGC TTACTGATAA GGTGCAGGCT
    GAGTATCGAG ACGCTTATAG AGAGTACATT GCTCAGATGT CTCAGTTAGA AGGGGGCACA
    GGTTCCACAA CCATGAGTGG CAGGTCTTCT CCACACAGCA CGTATTATAT GGGTCAGAGT
    TCATCCGGGG GCTCCATTCA CTCTAGTCTA GAACCCGAGA AAGGGAAGGA TAGTGAACCA
    AAGCAAGATG ATACACGGAA GTCATTTCTG ATGAAGAGGG GAGATGTTAT AGATTATTCC
    TCATCAGGAG TTTCCACCAA TGATGCCTCA CCCCTGGATC CTATTACCGA GGAAGATGAA
    AAATCTGATC AGTCAGGCAG TAAGCTTCTC CCAGGCAAGA AGTCCTCAGA AAGGTCAAGT
    CTCTTCCAGA CAGACCTGAA GCTGAAGGGA GGTGGACTGC GCTATCAGAA ACTCCCGAGT
    GACGAAGACG AGTCTGGGAC AGAAGAGTCA GACAACACTC CACTGCTCAA AGACGACAAA
    GACAAGAAAG CTGAAGGGAA AGCAGAGAGA GCATTGAAGT CTCCAGAGCA CAGTGCAGAG
    CCCATTCGAA CCTTCATCAA GGCCAAAGAG TACTTGTCAG ATGCCCTCCT TGACAAAAAG
    GACTCCTCTG ACTCAGGAGT GAGGTCCAAT GAGAGCTCGC CCAACCACTC CCTTCACAAC
    GAGGCGGCAG ATGACTCCCA GCTTGAAAAG GCCAACCTCA TAGAGCTCGA AGATGACCCC
    CACAGTGGAA GACGGGGAGT CCCGCACAGC CTGAGTGGCT TGCCAGACCC CCTCGTAGCC
    CGGATGTCCA TTTGCTCCGA GGACAAGAAG AGCCCTTCTG AATGCAGCTT GATAGCCAGC
    AGCCCCGAGG AAAACTGGCC AGCATGCCAC AAATCCTACA ACCTGAATCG CACCCCCAGC
    ACCGTGACCC TGAACAACAA CAGTGCCCCG GCTAACAGAG CCAACCAGAA TTCCGATGAG
    ACGGAGGGGC TGAGGGAGAC GCCTCAAGTC CTCCTGAGGT CAGGCTCCAG TTCCCACTCC
    ACCGCTCTGC AGAATGAGAA CCTGAAGAGC ATGGCACACA AGCGAGGCCA GCGTTCCAGC
    TACACGAGGC TCTCAAAGGA CTCCTCAGAG CTCCACGCAG TAGCCTCCTC CGATAGTACA
    GGTTTTGGAG AAGAAAGGGA GAGCATTCTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586702.1
    CDS213..5525
    Translation

    Target ORF information:

    RefSeq Version XM_003586654.5
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586654.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGAAGTTATC
    AAGGAGGATG CTGCTGAGGG CCTTTCTTCA CCCACAGACT CCTCGAGGGG ATCAGGCCCA
    GCCTCAGGCA CAATACCACT GAATTCGATG AACGTGGATG CAGTTTGTGA GAAACTGAAA
    CAAATTGAAG GGCTGGACCA GAGCATGCTG CCTCAGTACT GTGCAACCAT CAAAAAGGCG
    AACATAAATG GCCGTGTGTT GGCTCAGTGT AACATTGATG AACTGAAGAA GGAAATGAAT
    ATGAATTTTG GAGACTGGCA TCTTTTTAGA AGCACAATAT TAGAAATGAG AAATGCAGAA
    AACCAAGTGG TCCCTGAAGA CCCACGTTTA CTGAGTGAAA ACAGCAGCTC TGTTCCTCAC
    GGTGAACCTG CTCGTCGCTC CGCACACAGC GAGCTGCCCC ACACCGAGCT CTCCAGTCAG
    GCTCCCTACA CCCTGAACTT CAGCTTTGAA GAGCTGAACA CGCTCGGCCT GGACGAAGGG
    GCTCCTCGCC ACAGCAACCT GAGTTGGCAG TCACAAACTC GCAGAACCCC AAGTCTTTCC
    AGTCTCACTT CCCAGGACTC CAGTATTGAA ATTTCAAAGC TTACTGATAA GGTGCAGGCT
    GAGTATCGAG ACGCTTATAG AGAGTACATT GCTCAGATGT CTCAGTTAGA AGGGGGCACA
    GGTTCCACAA CCATGAGTGG CAGGTCTTCT CCACACAGCA CGTATTATAT GGGTCAGAGT
    TCATCCGGGG GCTCCATTCA CTCTAGTCTA GAACCCGAGA AAGGGAAGGA TAGTGAACCA
    AAGCAAGATG ATACACGGAA GTCATTTCTG ATGAAGAGGG GAGATGTTAT AGATTATTCC
    TCATCAGGAG TTTCCACCAA TGATGCCTCA CCCCTGGATC CTATTACCGA GGAAGATGAA
    AAATCTGATC AGTCAGGCAG TAAGCTTCTC CCAGGCAAGA AGTCCTCAGA AAGGTCAAGT
    CTCTTCCAGA CAGACCTGAA GCTGAAGGGA GGTGGACTGC GCTATCAGAA ACTCCCGAGT
    GACGAAGACG AGTCTGGGAC AGAAGAGTCA GACAACACTC CACTGCTCAA AGACGACAAA
    GACAAGAAAG CTGAAGGGAA AGCAGAGAGA GCATTGAAGT CTCCAGAGCA CAGTGCAGAG
    CCCATTCGAA CCTTCATCAA GGCCAAAGAG TACTTGTCAG ATGCCCTCCT TGACAAAAAG
    GACTCCTCTG ACTCAGGAGT GAGGTCCAAT GAGAGCTCGC CCAACCACTC CCTTCACAAC
    GAGGCGGCAG ATGACTCCCA GCTTGAAAAG GCCAACCTCA TAGAGCTCGA AGATGACCCC
    CACAGTGGAA GACGGGGAGT CCCGCACAGC CTGAGTGGCT TGCCAGACCC CCTCGTAGCC
    CGGATGTCCA TTTGCTCCGA GGACAAGAAG AGCCCTTCTG AATGCAGCTT GATAGCCAGC
    AGCCCCGAGG AAAACTGGCC AGCATGCCAC AAATCCTACA ACCTGAATCG CACCCCCAGC
    ACCGTGACCC TGAACAACAA CAGTGCCCCG GCTAACAGAG CCAACCAGAA TTCCGATGAG
    ACGGAGGGGC TGAGGGAGAC GCCTCAAGTC CTCCTGAGGT CAGGCTCCAG TTCCCACTCC
    ACCGCTCTGC AGAATGAGAA CCTGAAGAGC ATGGCACACA AGCGAGGCCA GCGTTCCAGC
    TACACGAGGC TCTCAAAGGA CTCCTCAGAG CTCCACGCAG TAGCCTCCTC CGATAGTACA
    GGTTTTGGAG AAGAAAGGGA GAGCATTCTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24274
    Clone ID Related Accession (Same CDS sequence) XM_003586654.5 , XM_003586654.4 , XM_003582816.4
    Accession Version XM_003586654.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586654.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGAAGTTATC
    AAGGAGGATG CTGCTGAGGG CCTTTCTTCA CCCACAGACT CCTCGAGGGG ATCAGGCCCA
    GCCTCAGGCA CAATACCACT GAATTCGATG AACGTGGATG CAGTTTGTGA GAAACTGAAA
    CAAATTGAAG GGCTGGACCA GAGCATGCTG CCTCAGTACT GTGCAACCAT CAAAAAGGCG
    AACATAAATG GCCGTGTGTT GGCTCAGTGT AACATTGATG AACTGAAGAA GGAAATGAAT
    ATGAATTTTG GAGACTGGCA TCTTTTTAGA AGCACAATAT TAGAAATGAG AAATGCAGAA
    AACCAAGTGG TCCCTGAAGA CCCACGTTTA CTGAGTGAAA ACAGCAGCTC TGTTCCTCAC
    GGTGAACCTG CTCGTCGCTC CGCACACAGC GAGCTGCCCC ACACCGAGCT CTCCAGTCAG
    GCTCCCTACA CCCTGAACTT CAGCTTTGAA GAGCTGAACA CGCTCGGCCT GGACGAAGGG
    GCTCCTCGCC ACAGCAACCT GAGTTGGCAG TCACAAACTC GCAGAACCCC AAGTCTTTCC
    AGTCTCACTT CCCAGGACTC CAGTATTGAA ATTTCAAAGC TTACTGATAA GGTGCAGGCT
    GAGTATCGAG ACGCTTATAG AGAGTACATT GCTCAGATGT CTCAGTTAGA AGGGGGCACA
    GGTTCCACAA CCATGAGTGG CAGGTCTTCT CCACACAGCA CGTATTATAT GGGTCAGAGT
    TCATCCGGGG GCTCCATTCA CTCTAGTCTA GAACCCGAGA AAGGGAAGGA TAGTGAACCA
    AAGCAAGATG ATACACGGAA GTCATTTCTG ATGAAGAGGG GAGATGTTAT AGATTATTCC
    TCATCAGGAG TTTCCACCAA TGATGCCTCA CCCCTGGATC CTATTACCGA GGAAGATGAA
    AAATCTGATC AGTCAGGCAG TAAGCTTCTC CCAGGCAAGA AGTCCTCAGA AAGGTCAAGT
    CTCTTCCAGA CAGACCTGAA GCTGAAGGGA GGTGGACTGC GCTATCAGAA ACTCCCGAGT
    GACGAAGACG AGTCTGGGAC AGAAGAGTCA GACAACACTC CACTGCTCAA AGACGACAAA
    GACAAGAAAG CTGAAGGGAA AGCAGAGAGA GCATTGAAGT CTCCAGAGCA CAGTGCAGAG
    CCCATTCGAA CCTTCATCAA GGCCAAAGAG TACTTGTCAG ATGCCCTCCT TGACAAAAAG
    GACTCCTCTG ACTCAGGAGT GAGGTCCAAT GAGAGCTCGC CCAACCACTC CCTTCACAAC
    GAGGCGGCAG ATGACTCCCA GCTTGAAAAG GCCAACCTCA TAGAGCTCGA AGATGACCCC
    CACAGTGGAA GACGGGGAGT CCCGCACAGC CTGAGTGGCT TGCCAGACCC CCTCGTAGCC
    CGGATGTCCA TTTGCTCCGA GGACAAGAAG AGCCCTTCTG AATGCAGCTT GATAGCCAGC
    AGCCCCGAGG AAAACTGGCC AGCATGCCAC AAATCCTACA ACCTGAATCG CACCCCCAGC
    ACCGTGACCC TGAACAACAA CAGTGCCCCG GCTAACAGAG CCAACCAGAA TTCCGATGAG
    ACGGAGGGGC TGAGGGAGAC GCCTCAAGTC CTCCTGAGGT CAGGCTCCAG TTCCCACTCC
    ACCGCTCTGC AGAATGAGAA CCTGAAGAGC ATGGCACACA AGCGAGGCCA GCGTTCCAGC
    TACACGAGGC TCTCAAAGGA CTCCTCAGAG CTCCACGCAG TAGCCTCCTC CGATAGTACA
    GGTTTTGGAG AAGAAAGGGA GAGCATTCTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586702.1
    CDS207..5519
    Translation

    Target ORF information:

    RefSeq Version XM_003586654.4
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586654.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGAAGTTATC
    AAGGAGGATG CTGCTGAGGG CCTTTCTTCA CCCACAGACT CCTCGAGGGG ATCAGGCCCA
    GCCTCAGGCA CAATACCACT GAATTCGATG AACGTGGATG CAGTTTGTGA GAAACTGAAA
    CAAATTGAAG GGCTGGACCA GAGCATGCTG CCTCAGTACT GTGCAACCAT CAAAAAGGCG
    AACATAAATG GCCGTGTGTT GGCTCAGTGT AACATTGATG AACTGAAGAA GGAAATGAAT
    ATGAATTTTG GAGACTGGCA TCTTTTTAGA AGCACAATAT TAGAAATGAG AAATGCAGAA
    AACCAAGTGG TCCCTGAAGA CCCACGTTTA CTGAGTGAAA ACAGCAGCTC TGTTCCTCAC
    GGTGAACCTG CTCGTCGCTC CGCACACAGC GAGCTGCCCC ACACCGAGCT CTCCAGTCAG
    GCTCCCTACA CCCTGAACTT CAGCTTTGAA GAGCTGAACA CGCTCGGCCT GGACGAAGGG
    GCTCCTCGCC ACAGCAACCT GAGTTGGCAG TCACAAACTC GCAGAACCCC AAGTCTTTCC
    AGTCTCACTT CCCAGGACTC CAGTATTGAA ATTTCAAAGC TTACTGATAA GGTGCAGGCT
    GAGTATCGAG ACGCTTATAG AGAGTACATT GCTCAGATGT CTCAGTTAGA AGGGGGCACA
    GGTTCCACAA CCATGAGTGG CAGGTCTTCT CCACACAGCA CGTATTATAT GGGTCAGAGT
    TCATCCGGGG GCTCCATTCA CTCTAGTCTA GAACCCGAGA AAGGGAAGGA TAGTGAACCA
    AAGCAAGATG ATACACGGAA GTCATTTCTG ATGAAGAGGG GAGATGTTAT AGATTATTCC
    TCATCAGGAG TTTCCACCAA TGATGCCTCA CCCCTGGATC CTATTACCGA GGAAGATGAA
    AAATCTGATC AGTCAGGCAG TAAGCTTCTC CCAGGCAAGA AGTCCTCAGA AAGGTCAAGT
    CTCTTCCAGA CAGACCTGAA GCTGAAGGGA GGTGGACTGC GCTATCAGAA ACTCCCGAGT
    GACGAAGACG AGTCTGGGAC AGAAGAGTCA GACAACACTC CACTGCTCAA AGACGACAAA
    GACAAGAAAG CTGAAGGGAA AGCAGAGAGA GCATTGAAGT CTCCAGAGCA CAGTGCAGAG
    CCCATTCGAA CCTTCATCAA GGCCAAAGAG TACTTGTCAG ATGCCCTCCT TGACAAAAAG
    GACTCCTCTG ACTCAGGAGT GAGGTCCAAT GAGAGCTCGC CCAACCACTC CCTTCACAAC
    GAGGCGGCAG ATGACTCCCA GCTTGAAAAG GCCAACCTCA TAGAGCTCGA AGATGACCCC
    CACAGTGGAA GACGGGGAGT CCCGCACAGC CTGAGTGGCT TGCCAGACCC CCTCGTAGCC
    CGGATGTCCA TTTGCTCCGA GGACAAGAAG AGCCCTTCTG AATGCAGCTT GATAGCCAGC
    AGCCCCGAGG AAAACTGGCC AGCATGCCAC AAATCCTACA ACCTGAATCG CACCCCCAGC
    ACCGTGACCC TGAACAACAA CAGTGCCCCG GCTAACAGAG CCAACCAGAA TTCCGATGAG
    ACGGAGGGGC TGAGGGAGAC GCCTCAAGTC CTCCTGAGGT CAGGCTCCAG TTCCCACTCC
    ACCGCTCTGC AGAATGAGAA CCTGAAGAGC ATGGCACACA AGCGAGGCCA GCGTTCCAGC
    TACACGAGGC TCTCAAAGGA CTCCTCAGAG CTCCACGCAG TAGCCTCCTC CGATAGTACA
    GGTTTTGGAG AAGAAAGGGA GAGCATTCTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24274
    Clone ID Related Accession (Same CDS sequence) XM_003586654.5 , XM_003586654.4 , XM_003582816.4
    Accession Version XM_003582816.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582816.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGAAGTTATC
    AAGGAGGATG CTGCTGAGGG CCTTTCTTCA CCCACAGACT CCTCGAGGGG ATCAGGCCCA
    GCCTCAGGCA CAATACCACT GAATTCGATG AACGTGGATG CAGTTTGTGA GAAACTGAAA
    CAAATTGAAG GGCTGGACCA GAGCATGCTG CCTCAGTACT GTGCAACCAT CAAAAAGGCG
    AACATAAATG GCCGTGTGTT GGCTCAGTGT AACATTGATG AACTGAAGAA GGAAATGAAT
    ATGAATTTTG GAGACTGGCA TCTTTTTAGA AGCACAATAT TAGAAATGAG AAATGCAGAA
    AACCAAGTGG TCCCTGAAGA CCCACGTTTA CTGAGTGAAA ACAGCAGCTC TGTTCCTCAC
    GGTGAACCTG CTCGTCGCTC CGCACACAGC GAGCTGCCCC ACACCGAGCT CTCCAGTCAG
    GCTCCCTACA CCCTGAACTT CAGCTTTGAA GAGCTGAACA CGCTCGGCCT GGACGAAGGG
    GCTCCTCGCC ACAGCAACCT GAGTTGGCAG TCACAAACTC GCAGAACCCC AAGTCTTTCC
    AGTCTCACTT CCCAGGACTC CAGTATTGAA ATTTCAAAGC TTACTGATAA GGTGCAGGCT
    GAGTATCGAG ACGCTTATAG AGAGTACATT GCTCAGATGT CTCAGTTAGA AGGGGGCACA
    GGTTCCACAA CCATGAGTGG CAGGTCTTCT CCACACAGCA CGTATTATAT GGGTCAGAGT
    TCATCCGGGG GCTCCATTCA CTCTAGTCTA GAACCCGAGA AAGGGAAGGA TAGTGAACCA
    AAGCAAGATG ATACACGGAA GTCATTTCTG ATGAAGAGGG GAGATGTTAT AGATTATTCC
    TCATCAGGAG TTTCCACCAA TGATGCCTCA CCCCTGGATC CTATTACCGA GGAAGATGAA
    AAATCTGATC AGTCAGGCAG TAAGCTTCTC CCAGGCAAGA AGTCCTCAGA AAGGTCAAGT
    CTCTTCCAGA CAGACCTGAA GCTGAAGGGA GGTGGACTGC GCTATCAGAA ACTCCCGAGT
    GACGAAGACG AGTCTGGGAC AGAAGAGTCA GACAACACTC CACTGCTCAA AGACGACAAA
    GACAAGAAAG CTGAAGGGAA AGCAGAGAGA GCATTGAAGT CTCCAGAGCA CAGTGCAGAG
    CCCATTCGAA CCTTCATCAA GGCCAAAGAG TACTTGTCAG ATGCCCTCCT TGACAAAAAG
    GACTCCTCTG ACTCAGGAGT GAGGTCCAAT GAGAGCTCGC CCAACCACTC CCTTCACAAC
    GAGGCGGCAG ATGACTCCCA GCTTGAAAAG GCCAACCTCA TAGAGCTCGA AGATGACCCC
    CACAGTGGAA GACGGGGAGT CCCGCACAGC CTGAGTGGCT TGCCAGACCC CCTCGTAGCC
    CGGATGTCCA TTTGCTCCGA GGACAAGAAG AGCCCTTCTG AATGCAGCTT GATAGCCAGC
    AGCCCCGAGG AAAACTGGCC AGCATGCCAC AAATCCTACA ACCTGAATCG CACCCCCAGC
    ACCGTGACCC TGAACAACAA CAGTGCCCCG GCTAACAGAG CCAACCAGAA TTCCGATGAG
    ACGGAGGGGC TGAGGGAGAC GCCTCAAGTC CTCCTGAGGT CAGGCTCCAG TTCCCACTCC
    ACCGCTCTGC AGAATGAGAA CCTGAAGAGC ATGGCACACA AGCGAGGCCA GCGTTCCAGC
    TACACGAGGC TCTCAAAGGA CTCCTCAGAG CTCCACGCAG TAGCCTCCTC CGATAGTACA
    GGTTTTGGAG AAGAAAGGGA GAGCATTCTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003582864.2
    CDS207..5519
    Translation

    Target ORF information:

    RefSeq Version XM_003582816.4
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003582816.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGAAGTTATC
    AAGGAGGATG CTGCTGAGGG CCTTTCTTCA CCCACAGACT CCTCGAGGGG ATCAGGCCCA
    GCCTCAGGCA CAATACCACT GAATTCGATG AACGTGGATG CAGTTTGTGA GAAACTGAAA
    CAAATTGAAG GGCTGGACCA GAGCATGCTG CCTCAGTACT GTGCAACCAT CAAAAAGGCG
    AACATAAATG GCCGTGTGTT GGCTCAGTGT AACATTGATG AACTGAAGAA GGAAATGAAT
    ATGAATTTTG GAGACTGGCA TCTTTTTAGA AGCACAATAT TAGAAATGAG AAATGCAGAA
    AACCAAGTGG TCCCTGAAGA CCCACGTTTA CTGAGTGAAA ACAGCAGCTC TGTTCCTCAC
    GGTGAACCTG CTCGTCGCTC CGCACACAGC GAGCTGCCCC ACACCGAGCT CTCCAGTCAG
    GCTCCCTACA CCCTGAACTT CAGCTTTGAA GAGCTGAACA CGCTCGGCCT GGACGAAGGG
    GCTCCTCGCC ACAGCAACCT GAGTTGGCAG TCACAAACTC GCAGAACCCC AAGTCTTTCC
    AGTCTCACTT CCCAGGACTC CAGTATTGAA ATTTCAAAGC TTACTGATAA GGTGCAGGCT
    GAGTATCGAG ACGCTTATAG AGAGTACATT GCTCAGATGT CTCAGTTAGA AGGGGGCACA
    GGTTCCACAA CCATGAGTGG CAGGTCTTCT CCACACAGCA CGTATTATAT GGGTCAGAGT
    TCATCCGGGG GCTCCATTCA CTCTAGTCTA GAACCCGAGA AAGGGAAGGA TAGTGAACCA
    AAGCAAGATG ATACACGGAA GTCATTTCTG ATGAAGAGGG GAGATGTTAT AGATTATTCC
    TCATCAGGAG TTTCCACCAA TGATGCCTCA CCCCTGGATC CTATTACCGA GGAAGATGAA
    AAATCTGATC AGTCAGGCAG TAAGCTTCTC CCAGGCAAGA AGTCCTCAGA AAGGTCAAGT
    CTCTTCCAGA CAGACCTGAA GCTGAAGGGA GGTGGACTGC GCTATCAGAA ACTCCCGAGT
    GACGAAGACG AGTCTGGGAC AGAAGAGTCA GACAACACTC CACTGCTCAA AGACGACAAA
    GACAAGAAAG CTGAAGGGAA AGCAGAGAGA GCATTGAAGT CTCCAGAGCA CAGTGCAGAG
    CCCATTCGAA CCTTCATCAA GGCCAAAGAG TACTTGTCAG ATGCCCTCCT TGACAAAAAG
    GACTCCTCTG ACTCAGGAGT GAGGTCCAAT GAGAGCTCGC CCAACCACTC CCTTCACAAC
    GAGGCGGCAG ATGACTCCCA GCTTGAAAAG GCCAACCTCA TAGAGCTCGA AGATGACCCC
    CACAGTGGAA GACGGGGAGT CCCGCACAGC CTGAGTGGCT TGCCAGACCC CCTCGTAGCC
    CGGATGTCCA TTTGCTCCGA GGACAAGAAG AGCCCTTCTG AATGCAGCTT GATAGCCAGC
    AGCCCCGAGG AAAACTGGCC AGCATGCCAC AAATCCTACA ACCTGAATCG CACCCCCAGC
    ACCGTGACCC TGAACAACAA CAGTGCCCCG GCTAACAGAG CCAACCAGAA TTCCGATGAG
    ACGGAGGGGC TGAGGGAGAC GCCTCAAGTC CTCCTGAGGT CAGGCTCCAG TTCCCACTCC
    ACCGCTCTGC AGAATGAGAA CCTGAAGAGC ATGGCACACA AGCGAGGCCA GCGTTCCAGC
    TACACGAGGC TCTCAAAGGA CTCCTCAGAG CTCCACGCAG TAGCCTCCTC CGATAGTACA
    GGTTTTGGAG AAGAAAGGGA GAGCATTCTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24275
    Clone ID Related Accession (Same CDS sequence) XM_005213104.3 , XM_015465443.1 , XM_005213104.4
    Accession Version XM_005213104.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213104.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGGATCA
    GGCCCAGCCT CAGGCACAAT ACCACTGAAT TCGATGAACG TGGATGCAGT TTGTGAGAAA
    CTGAAACAAA TTGAAGGGCT GGACCAGAGC ATGCTGCCTC AGTACTGTGC AACCATCAAA
    AAGGCGAACA TAAATGGCCG TGTGTTGGCT CAGTGTAACA TTGATGAACT GAAGAAGGAA
    ATGAATATGA ATTTTGGAGA CTGGCATCTT TTTAGAAGCA CAATATTAGA AATGAGAAAT
    GCAGAAAACC AAGTGGTCCC TGAAGACCCA CGTTTACTGA GTGAAAACAG CAGCTCTGTT
    CCTCACGGTG AACCTGCTCG TCGCTCCGCA CACAGCGAGC TGCCCCACAC CGAGCTCTCC
    AGTCAGGCTC CCTACACCCT GAACTTCAGC TTTGAAGAGC TGAACACGCT CGGCCTGGAC
    GAAGGGGCTC CTCGCCACAG CAACCTGAGT TGGCAGTCAC AAACTCGCAG AACCCCAAGT
    CTTTCCAGTC TCACTTCCCA GGACTCCAGT ATTGAAATTT CAAAGCTTAC TGATAAGGTG
    CAGGCTGAGT ATCGAGACGC TTATAGAGAG TACATTGCTC AGATGTCTCA GTTAGAAGGG
    GGCACAGGTT CCACAACCAT GAGTGGCAGG TCTTCTCCAC ACAGCACGTA TTATATGGGT
    CAGAGTTCAT CCGGGGGCTC CATTCACTCT AGTCTAGAAC CCGAGAAAGG GAAGGATAGT
    GAACCAAAGC AAGATGATAC ACGGAAGTCA TTTCTGATGA AGAGGGGAGA TGTTATAGAT
    TATTCCTCAT CAGGAGTTTC CACCAATGAT GCCTCACCCC TGGATCCTAT TACCGAGGAA
    GATGAAAAAT CTGATCAGTC AGGCAGTAAG CTTCTCCCAG GCAAGAAGTC CTCAGAAAGG
    TCAAGTCTCT TCCAGACAGA CCTGAAGCTG AAGGGAGGTG GACTGCGCTA TCAGAAACTC
    CCGAGTGACG AAGACGAGTC TGGGACAGAA GAGTCAGACA ACACTCCACT GCTCAAAGAC
    GACAAAGACA AGAAAGCTGA AGGGAAAGCA GAGAGAGCAT TGAAGTCTCC AGAGCACAGT
    GCAGAGCCCA TTCGAACCTT CATCAAGGCC AAAGAGTACT TGTCAGATGC CCTCCTTGAC
    AAAAAGGACT CCTCTGACTC AGGAGTGAGG TCCAATGAGA GCTCGCCCAA CCACTCCCTT
    CACAACGAGG CGGCAGATGA CTCCCAGCTT GAAAAGGCCA ACCTCATAGA GCTCGAAGAT
    GACCCCCACA GTGGAAGACG GGGAGTCCCG CACAGCCTGA GTGGCTTGCC AGACCCCCTC
    GTAGCCCGGA TGTCCATTTG CTCCGAGGAC AAGAAGAGCC CTTCTGAATG CAGCTTGATA
    GCCAGCAGCC CCGAGGAAAA CTGGCCAGCA TGCCACAAAT CCTACAACCT GAATCGCACC
    CCCAGCACCG TGACCCTGAA CAACAACAGT GCCCCGGCTA ACAGAGCCAA CCAGAATTCC
    GATGAGACGG AGGGGCTGAG GGAGACGCCT CAAGTCCTCC TGAGGTCAGG CTCCAGTTCC
    CACTCCACCG CTCTGCAGAA TGAGAACCTG AAGAGCATGG CACACAAGCG AGGCCAGCGT
    TCCAGCTACA CGAGGCTCTC AAAGGACTCC TCAGAGCTCC ACGCAGTAGC CTCCTCCGAT
    AGTACAGGTT TTGGAGAAGA AAGGGAGAGC ATTCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213161.1
    CDS207..5465
    Translation

    Target ORF information:

    RefSeq Version XM_005213104.3
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213104.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGGATCA
    GGCCCAGCCT CAGGCACAAT ACCACTGAAT TCGATGAACG TGGATGCAGT TTGTGAGAAA
    CTGAAACAAA TTGAAGGGCT GGACCAGAGC ATGCTGCCTC AGTACTGTGC AACCATCAAA
    AAGGCGAACA TAAATGGCCG TGTGTTGGCT CAGTGTAACA TTGATGAACT GAAGAAGGAA
    ATGAATATGA ATTTTGGAGA CTGGCATCTT TTTAGAAGCA CAATATTAGA AATGAGAAAT
    GCAGAAAACC AAGTGGTCCC TGAAGACCCA CGTTTACTGA GTGAAAACAG CAGCTCTGTT
    CCTCACGGTG AACCTGCTCG TCGCTCCGCA CACAGCGAGC TGCCCCACAC CGAGCTCTCC
    AGTCAGGCTC CCTACACCCT GAACTTCAGC TTTGAAGAGC TGAACACGCT CGGCCTGGAC
    GAAGGGGCTC CTCGCCACAG CAACCTGAGT TGGCAGTCAC AAACTCGCAG AACCCCAAGT
    CTTTCCAGTC TCACTTCCCA GGACTCCAGT ATTGAAATTT CAAAGCTTAC TGATAAGGTG
    CAGGCTGAGT ATCGAGACGC TTATAGAGAG TACATTGCTC AGATGTCTCA GTTAGAAGGG
    GGCACAGGTT CCACAACCAT GAGTGGCAGG TCTTCTCCAC ACAGCACGTA TTATATGGGT
    CAGAGTTCAT CCGGGGGCTC CATTCACTCT AGTCTAGAAC CCGAGAAAGG GAAGGATAGT
    GAACCAAAGC AAGATGATAC ACGGAAGTCA TTTCTGATGA AGAGGGGAGA TGTTATAGAT
    TATTCCTCAT CAGGAGTTTC CACCAATGAT GCCTCACCCC TGGATCCTAT TACCGAGGAA
    GATGAAAAAT CTGATCAGTC AGGCAGTAAG CTTCTCCCAG GCAAGAAGTC CTCAGAAAGG
    TCAAGTCTCT TCCAGACAGA CCTGAAGCTG AAGGGAGGTG GACTGCGCTA TCAGAAACTC
    CCGAGTGACG AAGACGAGTC TGGGACAGAA GAGTCAGACA ACACTCCACT GCTCAAAGAC
    GACAAAGACA AGAAAGCTGA AGGGAAAGCA GAGAGAGCAT TGAAGTCTCC AGAGCACAGT
    GCAGAGCCCA TTCGAACCTT CATCAAGGCC AAAGAGTACT TGTCAGATGC CCTCCTTGAC
    AAAAAGGACT CCTCTGACTC AGGAGTGAGG TCCAATGAGA GCTCGCCCAA CCACTCCCTT
    CACAACGAGG CGGCAGATGA CTCCCAGCTT GAAAAGGCCA ACCTCATAGA GCTCGAAGAT
    GACCCCCACA GTGGAAGACG GGGAGTCCCG CACAGCCTGA GTGGCTTGCC AGACCCCCTC
    GTAGCCCGGA TGTCCATTTG CTCCGAGGAC AAGAAGAGCC CTTCTGAATG CAGCTTGATA
    GCCAGCAGCC CCGAGGAAAA CTGGCCAGCA TGCCACAAAT CCTACAACCT GAATCGCACC
    CCCAGCACCG TGACCCTGAA CAACAACAGT GCCCCGGCTA ACAGAGCCAA CCAGAATTCC
    GATGAGACGG AGGGGCTGAG GGAGACGCCT CAAGTCCTCC TGAGGTCAGG CTCCAGTTCC
    CACTCCACCG CTCTGCAGAA TGAGAACCTG AAGAGCATGG CACACAAGCG AGGCCAGCGT
    TCCAGCTACA CGAGGCTCTC AAAGGACTCC TCAGAGCTCC ACGCAGTAGC CTCCTCCGAT
    AGTACAGGTT TTGGAGAAGA AAGGGAGAGC ATTCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24275
    Clone ID Related Accession (Same CDS sequence) XM_005213104.3 , XM_015465443.1 , XM_005213104.4
    Accession Version XM_015465443.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGGATCA
    GGCCCAGCCT CAGGCACAAT ACCACTGAAT TCGATGAACG TGGATGCAGT TTGTGAGAAA
    CTGAAACAAA TTGAAGGGCT GGACCAGAGC ATGCTGCCTC AGTACTGTGC AACCATCAAA
    AAGGCGAACA TAAATGGCCG TGTGTTGGCT CAGTGTAACA TTGATGAACT GAAGAAGGAA
    ATGAATATGA ATTTTGGAGA CTGGCATCTT TTTAGAAGCA CAATATTAGA AATGAGAAAT
    GCAGAAAACC AAGTGGTCCC TGAAGACCCA CGTTTACTGA GTGAAAACAG CAGCTCTGTT
    CCTCACGGTG AACCTGCTCG TCGCTCCGCA CACAGCGAGC TGCCCCACAC CGAGCTCTCC
    AGTCAGGCTC CCTACACCCT GAACTTCAGC TTTGAAGAGC TGAACACGCT CGGCCTGGAC
    GAAGGGGCTC CTCGCCACAG CAACCTGAGT TGGCAGTCAC AAACTCGCAG AACCCCAAGT
    CTTTCCAGTC TCACTTCCCA GGACTCCAGT ATTGAAATTT CAAAGCTTAC TGATAAGGTG
    CAGGCTGAGT ATCGAGACGC TTATAGAGAG TACATTGCTC AGATGTCTCA GTTAGAAGGG
    GGCACAGGTT CCACAACCAT GAGTGGCAGG TCTTCTCCAC ACAGCACGTA TTATATGGGT
    CAGAGTTCAT CCGGGGGCTC CATTCACTCT AGTCTAGAAC CCGAGAAAGG GAAGGATAGT
    GAACCAAAGC AAGATGATAC ACGGAAGTCA TTTCTGATGA AGAGGGGAGA TGTTATAGAT
    TATTCCTCAT CAGGAGTTTC CACCAATGAT GCCTCACCCC TGGATCCTAT TACCGAGGAA
    GATGAAAAAT CTGATCAGTC AGGCAGTAAG CTTCTCCCAG GCAAGAAGTC CTCAGAAAGG
    TCAAGTCTCT TCCAGACAGA CCTGAAGCTG AAGGGAGGTG GACTGCGCTA TCAGAAACTC
    CCGAGTGACG AAGACGAGTC TGGGACAGAA GAGTCAGACA ACACTCCACT GCTCAAAGAC
    GACAAAGACA AGAAAGCTGA AGGGAAAGCA GAGAGAGCAT TGAAGTCTCC AGAGCACAGT
    GCAGAGCCCA TTCGAACCTT CATCAAGGCC AAAGAGTACT TGTCAGATGC CCTCCTTGAC
    AAAAAGGACT CCTCTGACTC AGGAGTGAGG TCCAATGAGA GCTCGCCCAA CCACTCCCTT
    CACAACGAGG CGGCAGATGA CTCCCAGCTT GAAAAGGCCA ACCTCATAGA GCTCGAAGAT
    GACCCCCACA GTGGAAGACG GGGAGTCCCG CACAGCCTGA GTGGCTTGCC AGACCCCCTC
    GTAGCCCGGA TGTCCATTTG CTCCGAGGAC AAGAAGAGCC CTTCTGAATG CAGCTTGATA
    GCCAGCAGCC CCGAGGAAAA CTGGCCAGCA TGCCACAAAT CCTACAACCT GAATCGCACC
    CCCAGCACCG TGACCCTGAA CAACAACAGT GCCCCGGCTA ACAGAGCCAA CCAGAATTCC
    GATGAGACGG AGGGGCTGAG GGAGACGCCT CAAGTCCTCC TGAGGTCAGG CTCCAGTTCC
    CACTCCACCG CTCTGCAGAA TGAGAACCTG AAGAGCATGG CACACAAGCG AGGCCAGCGT
    TCCAGCTACA CGAGGCTCTC AAAGGACTCC TCAGAGCTCC ACGCAGTAGC CTCCTCCGAT
    AGTACAGGTT TTGGAGAAGA AAGGGAGAGC ATTCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320929.1
    CDS207..5465
    Translation

    Target ORF information:

    RefSeq Version XM_015465443.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465443.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGGATCA
    GGCCCAGCCT CAGGCACAAT ACCACTGAAT TCGATGAACG TGGATGCAGT TTGTGAGAAA
    CTGAAACAAA TTGAAGGGCT GGACCAGAGC ATGCTGCCTC AGTACTGTGC AACCATCAAA
    AAGGCGAACA TAAATGGCCG TGTGTTGGCT CAGTGTAACA TTGATGAACT GAAGAAGGAA
    ATGAATATGA ATTTTGGAGA CTGGCATCTT TTTAGAAGCA CAATATTAGA AATGAGAAAT
    GCAGAAAACC AAGTGGTCCC TGAAGACCCA CGTTTACTGA GTGAAAACAG CAGCTCTGTT
    CCTCACGGTG AACCTGCTCG TCGCTCCGCA CACAGCGAGC TGCCCCACAC CGAGCTCTCC
    AGTCAGGCTC CCTACACCCT GAACTTCAGC TTTGAAGAGC TGAACACGCT CGGCCTGGAC
    GAAGGGGCTC CTCGCCACAG CAACCTGAGT TGGCAGTCAC AAACTCGCAG AACCCCAAGT
    CTTTCCAGTC TCACTTCCCA GGACTCCAGT ATTGAAATTT CAAAGCTTAC TGATAAGGTG
    CAGGCTGAGT ATCGAGACGC TTATAGAGAG TACATTGCTC AGATGTCTCA GTTAGAAGGG
    GGCACAGGTT CCACAACCAT GAGTGGCAGG TCTTCTCCAC ACAGCACGTA TTATATGGGT
    CAGAGTTCAT CCGGGGGCTC CATTCACTCT AGTCTAGAAC CCGAGAAAGG GAAGGATAGT
    GAACCAAAGC AAGATGATAC ACGGAAGTCA TTTCTGATGA AGAGGGGAGA TGTTATAGAT
    TATTCCTCAT CAGGAGTTTC CACCAATGAT GCCTCACCCC TGGATCCTAT TACCGAGGAA
    GATGAAAAAT CTGATCAGTC AGGCAGTAAG CTTCTCCCAG GCAAGAAGTC CTCAGAAAGG
    TCAAGTCTCT TCCAGACAGA CCTGAAGCTG AAGGGAGGTG GACTGCGCTA TCAGAAACTC
    CCGAGTGACG AAGACGAGTC TGGGACAGAA GAGTCAGACA ACACTCCACT GCTCAAAGAC
    GACAAAGACA AGAAAGCTGA AGGGAAAGCA GAGAGAGCAT TGAAGTCTCC AGAGCACAGT
    GCAGAGCCCA TTCGAACCTT CATCAAGGCC AAAGAGTACT TGTCAGATGC CCTCCTTGAC
    AAAAAGGACT CCTCTGACTC AGGAGTGAGG TCCAATGAGA GCTCGCCCAA CCACTCCCTT
    CACAACGAGG CGGCAGATGA CTCCCAGCTT GAAAAGGCCA ACCTCATAGA GCTCGAAGAT
    GACCCCCACA GTGGAAGACG GGGAGTCCCG CACAGCCTGA GTGGCTTGCC AGACCCCCTC
    GTAGCCCGGA TGTCCATTTG CTCCGAGGAC AAGAAGAGCC CTTCTGAATG CAGCTTGATA
    GCCAGCAGCC CCGAGGAAAA CTGGCCAGCA TGCCACAAAT CCTACAACCT GAATCGCACC
    CCCAGCACCG TGACCCTGAA CAACAACAGT GCCCCGGCTA ACAGAGCCAA CCAGAATTCC
    GATGAGACGG AGGGGCTGAG GGAGACGCCT CAAGTCCTCC TGAGGTCAGG CTCCAGTTCC
    CACTCCACCG CTCTGCAGAA TGAGAACCTG AAGAGCATGG CACACAAGCG AGGCCAGCGT
    TCCAGCTACA CGAGGCTCTC AAAGGACTCC TCAGAGCTCC ACGCAGTAGC CTCCTCCGAT
    AGTACAGGTT TTGGAGAAGA AAGGGAGAGC ATTCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24275
    Clone ID Related Accession (Same CDS sequence) XM_005213104.3 , XM_015465443.1 , XM_005213104.4
    Accession Version XM_005213104.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213104.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGGATCA
    GGCCCAGCCT CAGGCACAAT ACCACTGAAT TCGATGAACG TGGATGCAGT TTGTGAGAAA
    CTGAAACAAA TTGAAGGGCT GGACCAGAGC ATGCTGCCTC AGTACTGTGC AACCATCAAA
    AAGGCGAACA TAAATGGCCG TGTGTTGGCT CAGTGTAACA TTGATGAACT GAAGAAGGAA
    ATGAATATGA ATTTTGGAGA CTGGCATCTT TTTAGAAGCA CAATATTAGA AATGAGAAAT
    GCAGAAAACC AAGTGGTCCC TGAAGACCCA CGTTTACTGA GTGAAAACAG CAGCTCTGTT
    CCTCACGGTG AACCTGCTCG TCGCTCCGCA CACAGCGAGC TGCCCCACAC CGAGCTCTCC
    AGTCAGGCTC CCTACACCCT GAACTTCAGC TTTGAAGAGC TGAACACGCT CGGCCTGGAC
    GAAGGGGCTC CTCGCCACAG CAACCTGAGT TGGCAGTCAC AAACTCGCAG AACCCCAAGT
    CTTTCCAGTC TCACTTCCCA GGACTCCAGT ATTGAAATTT CAAAGCTTAC TGATAAGGTG
    CAGGCTGAGT ATCGAGACGC TTATAGAGAG TACATTGCTC AGATGTCTCA GTTAGAAGGG
    GGCACAGGTT CCACAACCAT GAGTGGCAGG TCTTCTCCAC ACAGCACGTA TTATATGGGT
    CAGAGTTCAT CCGGGGGCTC CATTCACTCT AGTCTAGAAC CCGAGAAAGG GAAGGATAGT
    GAACCAAAGC AAGATGATAC ACGGAAGTCA TTTCTGATGA AGAGGGGAGA TGTTATAGAT
    TATTCCTCAT CAGGAGTTTC CACCAATGAT GCCTCACCCC TGGATCCTAT TACCGAGGAA
    GATGAAAAAT CTGATCAGTC AGGCAGTAAG CTTCTCCCAG GCAAGAAGTC CTCAGAAAGG
    TCAAGTCTCT TCCAGACAGA CCTGAAGCTG AAGGGAGGTG GACTGCGCTA TCAGAAACTC
    CCGAGTGACG AAGACGAGTC TGGGACAGAA GAGTCAGACA ACACTCCACT GCTCAAAGAC
    GACAAAGACA AGAAAGCTGA AGGGAAAGCA GAGAGAGCAT TGAAGTCTCC AGAGCACAGT
    GCAGAGCCCA TTCGAACCTT CATCAAGGCC AAAGAGTACT TGTCAGATGC CCTCCTTGAC
    AAAAAGGACT CCTCTGACTC AGGAGTGAGG TCCAATGAGA GCTCGCCCAA CCACTCCCTT
    CACAACGAGG CGGCAGATGA CTCCCAGCTT GAAAAGGCCA ACCTCATAGA GCTCGAAGAT
    GACCCCCACA GTGGAAGACG GGGAGTCCCG CACAGCCTGA GTGGCTTGCC AGACCCCCTC
    GTAGCCCGGA TGTCCATTTG CTCCGAGGAC AAGAAGAGCC CTTCTGAATG CAGCTTGATA
    GCCAGCAGCC CCGAGGAAAA CTGGCCAGCA TGCCACAAAT CCTACAACCT GAATCGCACC
    CCCAGCACCG TGACCCTGAA CAACAACAGT GCCCCGGCTA ACAGAGCCAA CCAGAATTCC
    GATGAGACGG AGGGGCTGAG GGAGACGCCT CAAGTCCTCC TGAGGTCAGG CTCCAGTTCC
    CACTCCACCG CTCTGCAGAA TGAGAACCTG AAGAGCATGG CACACAAGCG AGGCCAGCGT
    TCCAGCTACA CGAGGCTCTC AAAGGACTCC TCAGAGCTCC ACGCAGTAGC CTCCTCCGAT
    AGTACAGGTT TTGGAGAAGA AAGGGAGAGC ATTCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213161.1
    CDS213..5471
    Translation

    Target ORF information:

    RefSeq Version XM_005213104.4
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213104.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGGATCA
    GGCCCAGCCT CAGGCACAAT ACCACTGAAT TCGATGAACG TGGATGCAGT TTGTGAGAAA
    CTGAAACAAA TTGAAGGGCT GGACCAGAGC ATGCTGCCTC AGTACTGTGC AACCATCAAA
    AAGGCGAACA TAAATGGCCG TGTGTTGGCT CAGTGTAACA TTGATGAACT GAAGAAGGAA
    ATGAATATGA ATTTTGGAGA CTGGCATCTT TTTAGAAGCA CAATATTAGA AATGAGAAAT
    GCAGAAAACC AAGTGGTCCC TGAAGACCCA CGTTTACTGA GTGAAAACAG CAGCTCTGTT
    CCTCACGGTG AACCTGCTCG TCGCTCCGCA CACAGCGAGC TGCCCCACAC CGAGCTCTCC
    AGTCAGGCTC CCTACACCCT GAACTTCAGC TTTGAAGAGC TGAACACGCT CGGCCTGGAC
    GAAGGGGCTC CTCGCCACAG CAACCTGAGT TGGCAGTCAC AAACTCGCAG AACCCCAAGT
    CTTTCCAGTC TCACTTCCCA GGACTCCAGT ATTGAAATTT CAAAGCTTAC TGATAAGGTG
    CAGGCTGAGT ATCGAGACGC TTATAGAGAG TACATTGCTC AGATGTCTCA GTTAGAAGGG
    GGCACAGGTT CCACAACCAT GAGTGGCAGG TCTTCTCCAC ACAGCACGTA TTATATGGGT
    CAGAGTTCAT CCGGGGGCTC CATTCACTCT AGTCTAGAAC CCGAGAAAGG GAAGGATAGT
    GAACCAAAGC AAGATGATAC ACGGAAGTCA TTTCTGATGA AGAGGGGAGA TGTTATAGAT
    TATTCCTCAT CAGGAGTTTC CACCAATGAT GCCTCACCCC TGGATCCTAT TACCGAGGAA
    GATGAAAAAT CTGATCAGTC AGGCAGTAAG CTTCTCCCAG GCAAGAAGTC CTCAGAAAGG
    TCAAGTCTCT TCCAGACAGA CCTGAAGCTG AAGGGAGGTG GACTGCGCTA TCAGAAACTC
    CCGAGTGACG AAGACGAGTC TGGGACAGAA GAGTCAGACA ACACTCCACT GCTCAAAGAC
    GACAAAGACA AGAAAGCTGA AGGGAAAGCA GAGAGAGCAT TGAAGTCTCC AGAGCACAGT
    GCAGAGCCCA TTCGAACCTT CATCAAGGCC AAAGAGTACT TGTCAGATGC CCTCCTTGAC
    AAAAAGGACT CCTCTGACTC AGGAGTGAGG TCCAATGAGA GCTCGCCCAA CCACTCCCTT
    CACAACGAGG CGGCAGATGA CTCCCAGCTT GAAAAGGCCA ACCTCATAGA GCTCGAAGAT
    GACCCCCACA GTGGAAGACG GGGAGTCCCG CACAGCCTGA GTGGCTTGCC AGACCCCCTC
    GTAGCCCGGA TGTCCATTTG CTCCGAGGAC AAGAAGAGCC CTTCTGAATG CAGCTTGATA
    GCCAGCAGCC CCGAGGAAAA CTGGCCAGCA TGCCACAAAT CCTACAACCT GAATCGCACC
    CCCAGCACCG TGACCCTGAA CAACAACAGT GCCCCGGCTA ACAGAGCCAA CCAGAATTCC
    GATGAGACGG AGGGGCTGAG GGAGACGCCT CAAGTCCTCC TGAGGTCAGG CTCCAGTTCC
    CACTCCACCG CTCTGCAGAA TGAGAACCTG AAGAGCATGG CACACAAGCG AGGCCAGCGT
    TCCAGCTACA CGAGGCTCTC AAAGGACTCC TCAGAGCTCC ACGCAGTAGC CTCCTCCGAT
    AGTACAGGTT TTGGAGAAGA AAGGGAGAGC ATTCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24276
    Clone ID Related Accession (Same CDS sequence) XM_015465444.1 , XM_005213105.4 , XM_005213105.3
    Accession Version XM_015465444.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGTGCAGG CTGAGTATCG AGACGCTTAT AGAGAGTACA TTGCTCAGAT GTCTCAGTTA
    GAAGGGGGCA CAGGTTCCAC AACCATGAGT GGCAGGTCTT CTCCACACAG CACGTATTAT
    ATGGGTCAGA GTTCATCCGG GGGCTCCATT CACTCTAGTC TAGAACCCGA GAAAGGGAAG
    GATAGTGAAC CAAAGCAAGA TGATACACGG AAGTCATTTC TGATGAAGAG GGGAGATGTT
    ATAGATTATT CCTCATCAGG AGTTTCCACC AATGATGCCT CACCCCTGGA TCCTATTACC
    GAGGAAGATG AAAAATCTGA TCAGTCAGGC AGTAAGCTTC TCCCAGGCAA GAAGTCCTCA
    GAAAGGTCAA GTCTCTTCCA GACAGACCTG AAGCTGAAGG GAGGTGGACT GCGCTATCAG
    AAACTCCCGA GTGACGAAGA CGAGTCTGGG ACAGAAGAGT CAGACAACAC TCCACTGCTC
    AAAGACGACA AAGACAAGAA AGCTGAAGGG AAAGCAGAGA GAGCATTGAA GTCTCCAGAG
    CACAGTGCAG AGCCCATTCG AACCTTCATC AAGGCCAAAG AGTACTTGTC AGATGCCCTC
    CTTGACAAAA AGGACTCCTC TGACTCAGGA GTGAGGTCCA ATGAGAGCTC GCCCAACCAC
    TCCCTTCACA ACGAGGCGGC AGATGACTCC CAGCTTGAAA AGGCCAACCT CATAGAGCTC
    GAAGATGACC CCCACAGTGG AAGACGGGGA GTCCCGCACA GCCTGAGTGG CTTGCCAGAC
    CCCCTCGTAG CCCGGATGTC CATTTGCTCC GAGGACAAGA AGAGCCCTTC TGAATGCAGC
    TTGATAGCCA GCAGCCCCGA GGAAAACTGG CCAGCATGCC ACAAATCCTA CAACCTGAAT
    CGCACCCCCA GCACCGTGAC CCTGAACAAC AACAGTGCCC CGGCTAACAG AGCCAACCAG
    AATTCCGATG AGACGGAGGG GCTGAGGGAG ACGCCTCAAG TCCTCCTGAG GTCAGGCTCC
    AGTTCCCACT CCACCGCTCT GCAGAATGAG AACCTGAAGA GCATGGCACA CAAGCGAGGC
    CAGCGTTCCA GCTACACGAG GCTCTCAAAG GACTCCTCAG AGCTCCACGC AGTAGCCTCC
    TCCGATAGTA CAGGTTTTGG AGAAGAAAGG GAGAGCATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320930.1
    CDS207..5351
    Translation

    Target ORF information:

    RefSeq Version XM_015465444.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465444.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGTGCAGG CTGAGTATCG AGACGCTTAT AGAGAGTACA TTGCTCAGAT GTCTCAGTTA
    GAAGGGGGCA CAGGTTCCAC AACCATGAGT GGCAGGTCTT CTCCACACAG CACGTATTAT
    ATGGGTCAGA GTTCATCCGG GGGCTCCATT CACTCTAGTC TAGAACCCGA GAAAGGGAAG
    GATAGTGAAC CAAAGCAAGA TGATACACGG AAGTCATTTC TGATGAAGAG GGGAGATGTT
    ATAGATTATT CCTCATCAGG AGTTTCCACC AATGATGCCT CACCCCTGGA TCCTATTACC
    GAGGAAGATG AAAAATCTGA TCAGTCAGGC AGTAAGCTTC TCCCAGGCAA GAAGTCCTCA
    GAAAGGTCAA GTCTCTTCCA GACAGACCTG AAGCTGAAGG GAGGTGGACT GCGCTATCAG
    AAACTCCCGA GTGACGAAGA CGAGTCTGGG ACAGAAGAGT CAGACAACAC TCCACTGCTC
    AAAGACGACA AAGACAAGAA AGCTGAAGGG AAAGCAGAGA GAGCATTGAA GTCTCCAGAG
    CACAGTGCAG AGCCCATTCG AACCTTCATC AAGGCCAAAG AGTACTTGTC AGATGCCCTC
    CTTGACAAAA AGGACTCCTC TGACTCAGGA GTGAGGTCCA ATGAGAGCTC GCCCAACCAC
    TCCCTTCACA ACGAGGCGGC AGATGACTCC CAGCTTGAAA AGGCCAACCT CATAGAGCTC
    GAAGATGACC CCCACAGTGG AAGACGGGGA GTCCCGCACA GCCTGAGTGG CTTGCCAGAC
    CCCCTCGTAG CCCGGATGTC CATTTGCTCC GAGGACAAGA AGAGCCCTTC TGAATGCAGC
    TTGATAGCCA GCAGCCCCGA GGAAAACTGG CCAGCATGCC ACAAATCCTA CAACCTGAAT
    CGCACCCCCA GCACCGTGAC CCTGAACAAC AACAGTGCCC CGGCTAACAG AGCCAACCAG
    AATTCCGATG AGACGGAGGG GCTGAGGGAG ACGCCTCAAG TCCTCCTGAG GTCAGGCTCC
    AGTTCCCACT CCACCGCTCT GCAGAATGAG AACCTGAAGA GCATGGCACA CAAGCGAGGC
    CAGCGTTCCA GCTACACGAG GCTCTCAAAG GACTCCTCAG AGCTCCACGC AGTAGCCTCC
    TCCGATAGTA CAGGTTTTGG AGAAGAAAGG GAGAGCATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24276
    Clone ID Related Accession (Same CDS sequence) XM_015465444.1 , XM_005213105.4 , XM_005213105.3
    Accession Version XM_005213105.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213105.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGTGCAGG CTGAGTATCG AGACGCTTAT AGAGAGTACA TTGCTCAGAT GTCTCAGTTA
    GAAGGGGGCA CAGGTTCCAC AACCATGAGT GGCAGGTCTT CTCCACACAG CACGTATTAT
    ATGGGTCAGA GTTCATCCGG GGGCTCCATT CACTCTAGTC TAGAACCCGA GAAAGGGAAG
    GATAGTGAAC CAAAGCAAGA TGATACACGG AAGTCATTTC TGATGAAGAG GGGAGATGTT
    ATAGATTATT CCTCATCAGG AGTTTCCACC AATGATGCCT CACCCCTGGA TCCTATTACC
    GAGGAAGATG AAAAATCTGA TCAGTCAGGC AGTAAGCTTC TCCCAGGCAA GAAGTCCTCA
    GAAAGGTCAA GTCTCTTCCA GACAGACCTG AAGCTGAAGG GAGGTGGACT GCGCTATCAG
    AAACTCCCGA GTGACGAAGA CGAGTCTGGG ACAGAAGAGT CAGACAACAC TCCACTGCTC
    AAAGACGACA AAGACAAGAA AGCTGAAGGG AAAGCAGAGA GAGCATTGAA GTCTCCAGAG
    CACAGTGCAG AGCCCATTCG AACCTTCATC AAGGCCAAAG AGTACTTGTC AGATGCCCTC
    CTTGACAAAA AGGACTCCTC TGACTCAGGA GTGAGGTCCA ATGAGAGCTC GCCCAACCAC
    TCCCTTCACA ACGAGGCGGC AGATGACTCC CAGCTTGAAA AGGCCAACCT CATAGAGCTC
    GAAGATGACC CCCACAGTGG AAGACGGGGA GTCCCGCACA GCCTGAGTGG CTTGCCAGAC
    CCCCTCGTAG CCCGGATGTC CATTTGCTCC GAGGACAAGA AGAGCCCTTC TGAATGCAGC
    TTGATAGCCA GCAGCCCCGA GGAAAACTGG CCAGCATGCC ACAAATCCTA CAACCTGAAT
    CGCACCCCCA GCACCGTGAC CCTGAACAAC AACAGTGCCC CGGCTAACAG AGCCAACCAG
    AATTCCGATG AGACGGAGGG GCTGAGGGAG ACGCCTCAAG TCCTCCTGAG GTCAGGCTCC
    AGTTCCCACT CCACCGCTCT GCAGAATGAG AACCTGAAGA GCATGGCACA CAAGCGAGGC
    CAGCGTTCCA GCTACACGAG GCTCTCAAAG GACTCCTCAG AGCTCCACGC AGTAGCCTCC
    TCCGATAGTA CAGGTTTTGG AGAAGAAAGG GAGAGCATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213162.1
    CDS213..5357
    Translation

    Target ORF information:

    RefSeq Version XM_005213105.4
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213105.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGTGCAGG CTGAGTATCG AGACGCTTAT AGAGAGTACA TTGCTCAGAT GTCTCAGTTA
    GAAGGGGGCA CAGGTTCCAC AACCATGAGT GGCAGGTCTT CTCCACACAG CACGTATTAT
    ATGGGTCAGA GTTCATCCGG GGGCTCCATT CACTCTAGTC TAGAACCCGA GAAAGGGAAG
    GATAGTGAAC CAAAGCAAGA TGATACACGG AAGTCATTTC TGATGAAGAG GGGAGATGTT
    ATAGATTATT CCTCATCAGG AGTTTCCACC AATGATGCCT CACCCCTGGA TCCTATTACC
    GAGGAAGATG AAAAATCTGA TCAGTCAGGC AGTAAGCTTC TCCCAGGCAA GAAGTCCTCA
    GAAAGGTCAA GTCTCTTCCA GACAGACCTG AAGCTGAAGG GAGGTGGACT GCGCTATCAG
    AAACTCCCGA GTGACGAAGA CGAGTCTGGG ACAGAAGAGT CAGACAACAC TCCACTGCTC
    AAAGACGACA AAGACAAGAA AGCTGAAGGG AAAGCAGAGA GAGCATTGAA GTCTCCAGAG
    CACAGTGCAG AGCCCATTCG AACCTTCATC AAGGCCAAAG AGTACTTGTC AGATGCCCTC
    CTTGACAAAA AGGACTCCTC TGACTCAGGA GTGAGGTCCA ATGAGAGCTC GCCCAACCAC
    TCCCTTCACA ACGAGGCGGC AGATGACTCC CAGCTTGAAA AGGCCAACCT CATAGAGCTC
    GAAGATGACC CCCACAGTGG AAGACGGGGA GTCCCGCACA GCCTGAGTGG CTTGCCAGAC
    CCCCTCGTAG CCCGGATGTC CATTTGCTCC GAGGACAAGA AGAGCCCTTC TGAATGCAGC
    TTGATAGCCA GCAGCCCCGA GGAAAACTGG CCAGCATGCC ACAAATCCTA CAACCTGAAT
    CGCACCCCCA GCACCGTGAC CCTGAACAAC AACAGTGCCC CGGCTAACAG AGCCAACCAG
    AATTCCGATG AGACGGAGGG GCTGAGGGAG ACGCCTCAAG TCCTCCTGAG GTCAGGCTCC
    AGTTCCCACT CCACCGCTCT GCAGAATGAG AACCTGAAGA GCATGGCACA CAAGCGAGGC
    CAGCGTTCCA GCTACACGAG GCTCTCAAAG GACTCCTCAG AGCTCCACGC AGTAGCCTCC
    TCCGATAGTA CAGGTTTTGG AGAAGAAAGG GAGAGCATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24276
    Clone ID Related Accession (Same CDS sequence) XM_015465444.1 , XM_005213105.4 , XM_005213105.3
    Accession Version XM_005213105.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213105.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGTGCAGG CTGAGTATCG AGACGCTTAT AGAGAGTACA TTGCTCAGAT GTCTCAGTTA
    GAAGGGGGCA CAGGTTCCAC AACCATGAGT GGCAGGTCTT CTCCACACAG CACGTATTAT
    ATGGGTCAGA GTTCATCCGG GGGCTCCATT CACTCTAGTC TAGAACCCGA GAAAGGGAAG
    GATAGTGAAC CAAAGCAAGA TGATACACGG AAGTCATTTC TGATGAAGAG GGGAGATGTT
    ATAGATTATT CCTCATCAGG AGTTTCCACC AATGATGCCT CACCCCTGGA TCCTATTACC
    GAGGAAGATG AAAAATCTGA TCAGTCAGGC AGTAAGCTTC TCCCAGGCAA GAAGTCCTCA
    GAAAGGTCAA GTCTCTTCCA GACAGACCTG AAGCTGAAGG GAGGTGGACT GCGCTATCAG
    AAACTCCCGA GTGACGAAGA CGAGTCTGGG ACAGAAGAGT CAGACAACAC TCCACTGCTC
    AAAGACGACA AAGACAAGAA AGCTGAAGGG AAAGCAGAGA GAGCATTGAA GTCTCCAGAG
    CACAGTGCAG AGCCCATTCG AACCTTCATC AAGGCCAAAG AGTACTTGTC AGATGCCCTC
    CTTGACAAAA AGGACTCCTC TGACTCAGGA GTGAGGTCCA ATGAGAGCTC GCCCAACCAC
    TCCCTTCACA ACGAGGCGGC AGATGACTCC CAGCTTGAAA AGGCCAACCT CATAGAGCTC
    GAAGATGACC CCCACAGTGG AAGACGGGGA GTCCCGCACA GCCTGAGTGG CTTGCCAGAC
    CCCCTCGTAG CCCGGATGTC CATTTGCTCC GAGGACAAGA AGAGCCCTTC TGAATGCAGC
    TTGATAGCCA GCAGCCCCGA GGAAAACTGG CCAGCATGCC ACAAATCCTA CAACCTGAAT
    CGCACCCCCA GCACCGTGAC CCTGAACAAC AACAGTGCCC CGGCTAACAG AGCCAACCAG
    AATTCCGATG AGACGGAGGG GCTGAGGGAG ACGCCTCAAG TCCTCCTGAG GTCAGGCTCC
    AGTTCCCACT CCACCGCTCT GCAGAATGAG AACCTGAAGA GCATGGCACA CAAGCGAGGC
    CAGCGTTCCA GCTACACGAG GCTCTCAAAG GACTCCTCAG AGCTCCACGC AGTAGCCTCC
    TCCGATAGTA CAGGTTTTGG AGAAGAAAGG GAGAGCATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213162.1
    CDS207..5351
    Translation

    Target ORF information:

    RefSeq Version XM_005213105.3
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213105.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGTGCAGG CTGAGTATCG AGACGCTTAT AGAGAGTACA TTGCTCAGAT GTCTCAGTTA
    GAAGGGGGCA CAGGTTCCAC AACCATGAGT GGCAGGTCTT CTCCACACAG CACGTATTAT
    ATGGGTCAGA GTTCATCCGG GGGCTCCATT CACTCTAGTC TAGAACCCGA GAAAGGGAAG
    GATAGTGAAC CAAAGCAAGA TGATACACGG AAGTCATTTC TGATGAAGAG GGGAGATGTT
    ATAGATTATT CCTCATCAGG AGTTTCCACC AATGATGCCT CACCCCTGGA TCCTATTACC
    GAGGAAGATG AAAAATCTGA TCAGTCAGGC AGTAAGCTTC TCCCAGGCAA GAAGTCCTCA
    GAAAGGTCAA GTCTCTTCCA GACAGACCTG AAGCTGAAGG GAGGTGGACT GCGCTATCAG
    AAACTCCCGA GTGACGAAGA CGAGTCTGGG ACAGAAGAGT CAGACAACAC TCCACTGCTC
    AAAGACGACA AAGACAAGAA AGCTGAAGGG AAAGCAGAGA GAGCATTGAA GTCTCCAGAG
    CACAGTGCAG AGCCCATTCG AACCTTCATC AAGGCCAAAG AGTACTTGTC AGATGCCCTC
    CTTGACAAAA AGGACTCCTC TGACTCAGGA GTGAGGTCCA ATGAGAGCTC GCCCAACCAC
    TCCCTTCACA ACGAGGCGGC AGATGACTCC CAGCTTGAAA AGGCCAACCT CATAGAGCTC
    GAAGATGACC CCCACAGTGG AAGACGGGGA GTCCCGCACA GCCTGAGTGG CTTGCCAGAC
    CCCCTCGTAG CCCGGATGTC CATTTGCTCC GAGGACAAGA AGAGCCCTTC TGAATGCAGC
    TTGATAGCCA GCAGCCCCGA GGAAAACTGG CCAGCATGCC ACAAATCCTA CAACCTGAAT
    CGCACCCCCA GCACCGTGAC CCTGAACAAC AACAGTGCCC CGGCTAACAG AGCCAACCAG
    AATTCCGATG AGACGGAGGG GCTGAGGGAG ACGCCTCAAG TCCTCCTGAG GTCAGGCTCC
    AGTTCCCACT CCACCGCTCT GCAGAATGAG AACCTGAAGA GCATGGCACA CAAGCGAGGC
    CAGCGTTCCA GCTACACGAG GCTCTCAAAG GACTCCTCAG AGCTCCACGC AGTAGCCTCC
    TCCGATAGTA CAGGTTTTGG AGAAGAAAGG GAGAGCATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53363
    Clone ID Related Accession (Same CDS sequence) XM_015465437.1 , XM_015465438.1 , XM_010810305.2 , XM_015473572.1
    Accession Version XM_015465437.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGTGCAGG CTGAGTATCG AGACGCTTAT AGAGAGTACA TTGCTCAGAT GTCTCAGTTA
    GAAGGGGGCA CAGGTTCCAC AACCATGAGT GGCAGGTCTT CTCCACACAG CACGTATTAT
    ATGGGTCAGA GTTCATCCGG GGGCTCCATT CACTCTAGTC TAGAACCCGA GAAAGGGAAG
    GATAGTGAAC CAAAGCAAGA TGATACACGG AAGTCATTTC TGATGAAGAG GGGAGATGTT
    ATAGATTATT CCTCATCAGG AGTTTCCACC AATGATGCCT CACCCCTGGA TCCTATTACC
    GAGGAAGATG AAAAATCTGA TCAGTCAGGC AGTAAGCTTC TCCCAGGCAA GAAGTCCTCA
    GAAAGGTCAA GTCTCTTCCA GACAGACCTG AAGCTGAAGG GAGGTGGACT GCGCTATCAG
    AAACTCCCGA GTGACGAAGA CGAGTCTGGG ACAGAAGAGT CAGACAACAC TCCACTGCTC
    AAAGACGACA AAGACAAGAA AGCTGAAGGG AAAGCAGAGA GAGCATTGAA GTCTCCAGAG
    CACAGTGCAG AGCCCATTCG AACCTTCATC AAGGCCAAAG AGTACTTGTC AGATGCCCTC
    CTTGACAAAA AGGACTCCTC TGACTCAGGA GTGAGGTCCA ATGAGAGCTC GCCCAACCAC
    TCCCTTCACA ACGAGGCGGC AGATGACTCC CAGCTTGAAA AGGCCAACCT CATAGAGCTC
    GAAGATGACC CCCACAGTGG AAGACGGGGA GTCCCGCACA GCCTGAGTGG CTTGCCAGAC
    CCCCTCGTAG CCCGGATGTC CATTTGCTCC GAGGACAAGA AGAGCCCTTC TGAATGCAGC
    TTGATAGCCA GCAGCCCCGA GGAAAACTGG CCAGCATGCC ACAAATCCTA CAACCTGAAT
    CGCACCCCCA GCACCGTGAC CCTGAACAAC AACAGTGCCC CGGCTAACAG AGCCAACCAG
    AATTCCGATG AGACGGAGGG GCTGAGGGAG ACGCCTCAAG TCCTCCTGAG GTCAGGCTCC
    AGTTCCCACT CCACCGCTCT GCAGAATGAG AACCTGAAGA GCATGGCACA CAAGCGAGGC
    CAGCGTTCCA GCTACACGAG GCTCTCAAAG GACTCCTCAG AGCTCCACGC AGTAGCCTCC
    TCCGATAGTA CAGGTTTTGG AGAAGAAAGG GAGAGCATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320923.1
    CDS208..5592
    Translation

    Target ORF information:

    RefSeq Version XM_015465437.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465437.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGTGCAGG CTGAGTATCG AGACGCTTAT AGAGAGTACA TTGCTCAGAT GTCTCAGTTA
    GAAGGGGGCA CAGGTTCCAC AACCATGAGT GGCAGGTCTT CTCCACACAG CACGTATTAT
    ATGGGTCAGA GTTCATCCGG GGGCTCCATT CACTCTAGTC TAGAACCCGA GAAAGGGAAG
    GATAGTGAAC CAAAGCAAGA TGATACACGG AAGTCATTTC TGATGAAGAG GGGAGATGTT
    ATAGATTATT CCTCATCAGG AGTTTCCACC AATGATGCCT CACCCCTGGA TCCTATTACC
    GAGGAAGATG AAAAATCTGA TCAGTCAGGC AGTAAGCTTC TCCCAGGCAA GAAGTCCTCA
    GAAAGGTCAA GTCTCTTCCA GACAGACCTG AAGCTGAAGG GAGGTGGACT GCGCTATCAG
    AAACTCCCGA GTGACGAAGA CGAGTCTGGG ACAGAAGAGT CAGACAACAC TCCACTGCTC
    AAAGACGACA AAGACAAGAA AGCTGAAGGG AAAGCAGAGA GAGCATTGAA GTCTCCAGAG
    CACAGTGCAG AGCCCATTCG AACCTTCATC AAGGCCAAAG AGTACTTGTC AGATGCCCTC
    CTTGACAAAA AGGACTCCTC TGACTCAGGA GTGAGGTCCA ATGAGAGCTC GCCCAACCAC
    TCCCTTCACA ACGAGGCGGC AGATGACTCC CAGCTTGAAA AGGCCAACCT CATAGAGCTC
    GAAGATGACC CCCACAGTGG AAGACGGGGA GTCCCGCACA GCCTGAGTGG CTTGCCAGAC
    CCCCTCGTAG CCCGGATGTC CATTTGCTCC GAGGACAAGA AGAGCCCTTC TGAATGCAGC
    TTGATAGCCA GCAGCCCCGA GGAAAACTGG CCAGCATGCC ACAAATCCTA CAACCTGAAT
    CGCACCCCCA GCACCGTGAC CCTGAACAAC AACAGTGCCC CGGCTAACAG AGCCAACCAG
    AATTCCGATG AGACGGAGGG GCTGAGGGAG ACGCCTCAAG TCCTCCTGAG GTCAGGCTCC
    AGTTCCCACT CCACCGCTCT GCAGAATGAG AACCTGAAGA GCATGGCACA CAAGCGAGGC
    CAGCGTTCCA GCTACACGAG GCTCTCAAAG GACTCCTCAG AGCTCCACGC AGTAGCCTCC
    TCCGATAGTA CAGGTTTTGG AGAAGAAAGG GAGAGCATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53363
    Clone ID Related Accession (Same CDS sequence) XM_015465437.1 , XM_015465438.1 , XM_010810305.2 , XM_015473572.1
    Accession Version XM_015465438.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGTGCAGG CTGAGTATCG AGACGCTTAT AGAGAGTACA TTGCTCAGAT GTCTCAGTTA
    GAAGGGGGCA CAGGTTCCAC AACCATGAGT GGCAGGTCTT CTCCACACAG CACGTATTAT
    ATGGGTCAGA GTTCATCCGG GGGCTCCATT CACTCTAGTC TAGAACCCGA GAAAGGGAAG
    GATAGTGAAC CAAAGCAAGA TGATACACGG AAGTCATTTC TGATGAAGAG GGGAGATGTT
    ATAGATTATT CCTCATCAGG AGTTTCCACC AATGATGCCT CACCCCTGGA TCCTATTACC
    GAGGAAGATG AAAAATCTGA TCAGTCAGGC AGTAAGCTTC TCCCAGGCAA GAAGTCCTCA
    GAAAGGTCAA GTCTCTTCCA GACAGACCTG AAGCTGAAGG GAGGTGGACT GCGCTATCAG
    AAACTCCCGA GTGACGAAGA CGAGTCTGGG ACAGAAGAGT CAGACAACAC TCCACTGCTC
    AAAGACGACA AAGACAAGAA AGCTGAAGGG AAAGCAGAGA GAGCATTGAA GTCTCCAGAG
    CACAGTGCAG AGCCCATTCG AACCTTCATC AAGGCCAAAG AGTACTTGTC AGATGCCCTC
    CTTGACAAAA AGGACTCCTC TGACTCAGGA GTGAGGTCCA ATGAGAGCTC GCCCAACCAC
    TCCCTTCACA ACGAGGCGGC AGATGACTCC CAGCTTGAAA AGGCCAACCT CATAGAGCTC
    GAAGATGACC CCCACAGTGG AAGACGGGGA GTCCCGCACA GCCTGAGTGG CTTGCCAGAC
    CCCCTCGTAG CCCGGATGTC CATTTGCTCC GAGGACAAGA AGAGCCCTTC TGAATGCAGC
    TTGATAGCCA GCAGCCCCGA GGAAAACTGG CCAGCATGCC ACAAATCCTA CAACCTGAAT
    CGCACCCCCA GCACCGTGAC CCTGAACAAC AACAGTGCCC CGGCTAACAG AGCCAACCAG
    AATTCCGATG AGACGGAGGG GCTGAGGGAG ACGCCTCAAG TCCTCCTGAG GTCAGGCTCC
    AGTTCCCACT CCACCGCTCT GCAGAATGAG AACCTGAAGA GCATGGCACA CAAGCGAGGC
    CAGCGTTCCA GCTACACGAG GCTCTCAAAG GACTCCTCAG AGCTCCACGC AGTAGCCTCC
    TCCGATAGTA CAGGTTTTGG AGAAGAAAGG GAGAGCATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320924.1
    CDS304..5688
    Translation

    Target ORF information:

    RefSeq Version XM_015465438.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465438.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGTGCAGG CTGAGTATCG AGACGCTTAT AGAGAGTACA TTGCTCAGAT GTCTCAGTTA
    GAAGGGGGCA CAGGTTCCAC AACCATGAGT GGCAGGTCTT CTCCACACAG CACGTATTAT
    ATGGGTCAGA GTTCATCCGG GGGCTCCATT CACTCTAGTC TAGAACCCGA GAAAGGGAAG
    GATAGTGAAC CAAAGCAAGA TGATACACGG AAGTCATTTC TGATGAAGAG GGGAGATGTT
    ATAGATTATT CCTCATCAGG AGTTTCCACC AATGATGCCT CACCCCTGGA TCCTATTACC
    GAGGAAGATG AAAAATCTGA TCAGTCAGGC AGTAAGCTTC TCCCAGGCAA GAAGTCCTCA
    GAAAGGTCAA GTCTCTTCCA GACAGACCTG AAGCTGAAGG GAGGTGGACT GCGCTATCAG
    AAACTCCCGA GTGACGAAGA CGAGTCTGGG ACAGAAGAGT CAGACAACAC TCCACTGCTC
    AAAGACGACA AAGACAAGAA AGCTGAAGGG AAAGCAGAGA GAGCATTGAA GTCTCCAGAG
    CACAGTGCAG AGCCCATTCG AACCTTCATC AAGGCCAAAG AGTACTTGTC AGATGCCCTC
    CTTGACAAAA AGGACTCCTC TGACTCAGGA GTGAGGTCCA ATGAGAGCTC GCCCAACCAC
    TCCCTTCACA ACGAGGCGGC AGATGACTCC CAGCTTGAAA AGGCCAACCT CATAGAGCTC
    GAAGATGACC CCCACAGTGG AAGACGGGGA GTCCCGCACA GCCTGAGTGG CTTGCCAGAC
    CCCCTCGTAG CCCGGATGTC CATTTGCTCC GAGGACAAGA AGAGCCCTTC TGAATGCAGC
    TTGATAGCCA GCAGCCCCGA GGAAAACTGG CCAGCATGCC ACAAATCCTA CAACCTGAAT
    CGCACCCCCA GCACCGTGAC CCTGAACAAC AACAGTGCCC CGGCTAACAG AGCCAACCAG
    AATTCCGATG AGACGGAGGG GCTGAGGGAG ACGCCTCAAG TCCTCCTGAG GTCAGGCTCC
    AGTTCCCACT CCACCGCTCT GCAGAATGAG AACCTGAAGA GCATGGCACA CAAGCGAGGC
    CAGCGTTCCA GCTACACGAG GCTCTCAAAG GACTCCTCAG AGCTCCACGC AGTAGCCTCC
    TCCGATAGTA CAGGTTTTGG AGAAGAAAGG GAGAGCATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53363
    Clone ID Related Accession (Same CDS sequence) XM_015465437.1 , XM_015465438.1 , XM_010810305.2 , XM_015473572.1
    Accession Version XM_010810305.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810305.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGTGCAGG CTGAGTATCG AGACGCTTAT AGAGAGTACA TTGCTCAGAT GTCTCAGTTA
    GAAGGGGGCA CAGGTTCCAC AACCATGAGT GGCAGGTCTT CTCCACACAG CACGTATTAT
    ATGGGTCAGA GTTCATCCGG GGGCTCCATT CACTCTAGTC TAGAACCCGA GAAAGGGAAG
    GATAGTGAAC CAAAGCAAGA TGATACACGG AAGTCATTTC TGATGAAGAG GGGAGATGTT
    ATAGATTATT CCTCATCAGG AGTTTCCACC AATGATGCCT CACCCCTGGA TCCTATTACC
    GAGGAAGATG AAAAATCTGA TCAGTCAGGC AGTAAGCTTC TCCCAGGCAA GAAGTCCTCA
    GAAAGGTCAA GTCTCTTCCA GACAGACCTG AAGCTGAAGG GAGGTGGACT GCGCTATCAG
    AAACTCCCGA GTGACGAAGA CGAGTCTGGG ACAGAAGAGT CAGACAACAC TCCACTGCTC
    AAAGACGACA AAGACAAGAA AGCTGAAGGG AAAGCAGAGA GAGCATTGAA GTCTCCAGAG
    CACAGTGCAG AGCCCATTCG AACCTTCATC AAGGCCAAAG AGTACTTGTC AGATGCCCTC
    CTTGACAAAA AGGACTCCTC TGACTCAGGA GTGAGGTCCA ATGAGAGCTC GCCCAACCAC
    TCCCTTCACA ACGAGGCGGC AGATGACTCC CAGCTTGAAA AGGCCAACCT CATAGAGCTC
    GAAGATGACC CCCACAGTGG AAGACGGGGA GTCCCGCACA GCCTGAGTGG CTTGCCAGAC
    CCCCTCGTAG CCCGGATGTC CATTTGCTCC GAGGACAAGA AGAGCCCTTC TGAATGCAGC
    TTGATAGCCA GCAGCCCCGA GGAAAACTGG CCAGCATGCC ACAAATCCTA CAACCTGAAT
    CGCACCCCCA GCACCGTGAC CCTGAACAAC AACAGTGCCC CGGCTAACAG AGCCAACCAG
    AATTCCGATG AGACGGAGGG GCTGAGGGAG ACGCCTCAAG TCCTCCTGAG GTCAGGCTCC
    AGTTCCCACT CCACCGCTCT GCAGAATGAG AACCTGAAGA GCATGGCACA CAAGCGAGGC
    CAGCGTTCCA GCTACACGAG GCTCTCAAAG GACTCCTCAG AGCTCCACGC AGTAGCCTCC
    TCCGATAGTA CAGGTTTTGG AGAAGAAAGG GAGAGCATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808607.1
    CDS208..5592
    Translation

    Target ORF information:

    RefSeq Version XM_010810305.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810305.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGTGCAGG CTGAGTATCG AGACGCTTAT AGAGAGTACA TTGCTCAGAT GTCTCAGTTA
    GAAGGGGGCA CAGGTTCCAC AACCATGAGT GGCAGGTCTT CTCCACACAG CACGTATTAT
    ATGGGTCAGA GTTCATCCGG GGGCTCCATT CACTCTAGTC TAGAACCCGA GAAAGGGAAG
    GATAGTGAAC CAAAGCAAGA TGATACACGG AAGTCATTTC TGATGAAGAG GGGAGATGTT
    ATAGATTATT CCTCATCAGG AGTTTCCACC AATGATGCCT CACCCCTGGA TCCTATTACC
    GAGGAAGATG AAAAATCTGA TCAGTCAGGC AGTAAGCTTC TCCCAGGCAA GAAGTCCTCA
    GAAAGGTCAA GTCTCTTCCA GACAGACCTG AAGCTGAAGG GAGGTGGACT GCGCTATCAG
    AAACTCCCGA GTGACGAAGA CGAGTCTGGG ACAGAAGAGT CAGACAACAC TCCACTGCTC
    AAAGACGACA AAGACAAGAA AGCTGAAGGG AAAGCAGAGA GAGCATTGAA GTCTCCAGAG
    CACAGTGCAG AGCCCATTCG AACCTTCATC AAGGCCAAAG AGTACTTGTC AGATGCCCTC
    CTTGACAAAA AGGACTCCTC TGACTCAGGA GTGAGGTCCA ATGAGAGCTC GCCCAACCAC
    TCCCTTCACA ACGAGGCGGC AGATGACTCC CAGCTTGAAA AGGCCAACCT CATAGAGCTC
    GAAGATGACC CCCACAGTGG AAGACGGGGA GTCCCGCACA GCCTGAGTGG CTTGCCAGAC
    CCCCTCGTAG CCCGGATGTC CATTTGCTCC GAGGACAAGA AGAGCCCTTC TGAATGCAGC
    TTGATAGCCA GCAGCCCCGA GGAAAACTGG CCAGCATGCC ACAAATCCTA CAACCTGAAT
    CGCACCCCCA GCACCGTGAC CCTGAACAAC AACAGTGCCC CGGCTAACAG AGCCAACCAG
    AATTCCGATG AGACGGAGGG GCTGAGGGAG ACGCCTCAAG TCCTCCTGAG GTCAGGCTCC
    AGTTCCCACT CCACCGCTCT GCAGAATGAG AACCTGAAGA GCATGGCACA CAAGCGAGGC
    CAGCGTTCCA GCTACACGAG GCTCTCAAAG GACTCCTCAG AGCTCCACGC AGTAGCCTCC
    TCCGATAGTA CAGGTTTTGG AGAAGAAAGG GAGAGCATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53363
    Clone ID Related Accession (Same CDS sequence) XM_015465437.1 , XM_015465438.1 , XM_010810305.2 , XM_015473572.1
    Accession Version XM_015473572.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGTGCAGG CTGAGTATCG AGACGCTTAT AGAGAGTACA TTGCTCAGAT GTCTCAGTTA
    GAAGGGGGCA CAGGTTCCAC AACCATGAGT GGCAGGTCTT CTCCACACAG CACGTATTAT
    ATGGGTCAGA GTTCATCCGG GGGCTCCATT CACTCTAGTC TAGAACCCGA GAAAGGGAAG
    GATAGTGAAC CAAAGCAAGA TGATACACGG AAGTCATTTC TGATGAAGAG GGGAGATGTT
    ATAGATTATT CCTCATCAGG AGTTTCCACC AATGATGCCT CACCCCTGGA TCCTATTACC
    GAGGAAGATG AAAAATCTGA TCAGTCAGGC AGTAAGCTTC TCCCAGGCAA GAAGTCCTCA
    GAAAGGTCAA GTCTCTTCCA GACAGACCTG AAGCTGAAGG GAGGTGGACT GCGCTATCAG
    AAACTCCCGA GTGACGAAGA CGAGTCTGGG ACAGAAGAGT CAGACAACAC TCCACTGCTC
    AAAGACGACA AAGACAAGAA AGCTGAAGGG AAAGCAGAGA GAGCATTGAA GTCTCCAGAG
    CACAGTGCAG AGCCCATTCG AACCTTCATC AAGGCCAAAG AGTACTTGTC AGATGCCCTC
    CTTGACAAAA AGGACTCCTC TGACTCAGGA GTGAGGTCCA ATGAGAGCTC GCCCAACCAC
    TCCCTTCACA ACGAGGCGGC AGATGACTCC CAGCTTGAAA AGGCCAACCT CATAGAGCTC
    GAAGATGACC CCCACAGTGG AAGACGGGGA GTCCCGCACA GCCTGAGTGG CTTGCCAGAC
    CCCCTCGTAG CCCGGATGTC CATTTGCTCC GAGGACAAGA AGAGCCCTTC TGAATGCAGC
    TTGATAGCCA GCAGCCCCGA GGAAAACTGG CCAGCATGCC ACAAATCCTA CAACCTGAAT
    CGCACCCCCA GCACCGTGAC CCTGAACAAC AACAGTGCCC CGGCTAACAG AGCCAACCAG
    AATTCCGATG AGACGGAGGG GCTGAGGGAG ACGCCTCAAG TCCTCCTGAG GTCAGGCTCC
    AGTTCCCACT CCACCGCTCT GCAGAATGAG AACCTGAAGA GCATGGCACA CAAGCGAGGC
    CAGCGTTCCA GCTACACGAG GCTCTCAAAG GACTCCTCAG AGCTCCACGC AGTAGCCTCC
    TCCGATAGTA CAGGTTTTGG AGAAGAAAGG GAGAGCATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329058.1
    CDS304..5688
    Translation

    Target ORF information:

    RefSeq Version XM_015473572.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473572.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTAGAAGTT
    ATCAAGGAGG ATGCTGCTGA GGGCCTTTCT TCACCCACAG ACTCCTCGAG GGAGCAAACT
    TGGACGAGAA AGCTAAAGAT GCCATGGCCG TGTGACTCTG GGTTTAACAA ACAGAGACAG
    GGATCAGGCC CAGCCTCAGG CACAATACCA CTGAATTCGA TGAACGTGGA TGCAGTTTGT
    GAGAAACTGA AACAAATTGA AGGGCTGGAC CAGAGCATGC TGCCTCAGTA CTGTGCAACC
    ATCAAAAAGG CGAACATAAA TGGCCGTGTG TTGGCTCAGT GTAACATTGA TGAACTGAAG
    AAGGAAATGA ATATGAATTT TGGAGACTGG CATCTTTTTA GAAGCACAAT ATTAGAAATG
    AGAAATGCAG AAAACCAAGT GGTCCCTGAA GACCCACGTT TACTGAGTGA AAACAGCAGC
    TCTGTTCCTC ACGGTGAACC TGCTCGTCGC TCCGCACACA GCGAGCTGCC CCACACCGAG
    CTCTCCAGTC AGGCTCCCTA CACCCTGAAC TTCAGCTTTG AAGAGCTGAA CACGCTCGGC
    CTGGACGAAG GGGCTCCTCG CCACAGCAAC CTGAGTTGGC AGTCACAAAC TCGCAGAACC
    CCAAGTCTTT CCAGTCTCAC TTCCCAGGAC TCCAGTATTG AAATTTCAAA GCTTACTGAT
    AAGGTGCAGG CTGAGTATCG AGACGCTTAT AGAGAGTACA TTGCTCAGAT GTCTCAGTTA
    GAAGGGGGCA CAGGTTCCAC AACCATGAGT GGCAGGTCTT CTCCACACAG CACGTATTAT
    ATGGGTCAGA GTTCATCCGG GGGCTCCATT CACTCTAGTC TAGAACCCGA GAAAGGGAAG
    GATAGTGAAC CAAAGCAAGA TGATACACGG AAGTCATTTC TGATGAAGAG GGGAGATGTT
    ATAGATTATT CCTCATCAGG AGTTTCCACC AATGATGCCT CACCCCTGGA TCCTATTACC
    GAGGAAGATG AAAAATCTGA TCAGTCAGGC AGTAAGCTTC TCCCAGGCAA GAAGTCCTCA
    GAAAGGTCAA GTCTCTTCCA GACAGACCTG AAGCTGAAGG GAGGTGGACT GCGCTATCAG
    AAACTCCCGA GTGACGAAGA CGAGTCTGGG ACAGAAGAGT CAGACAACAC TCCACTGCTC
    AAAGACGACA AAGACAAGAA AGCTGAAGGG AAAGCAGAGA GAGCATTGAA GTCTCCAGAG
    CACAGTGCAG AGCCCATTCG AACCTTCATC AAGGCCAAAG AGTACTTGTC AGATGCCCTC
    CTTGACAAAA AGGACTCCTC TGACTCAGGA GTGAGGTCCA ATGAGAGCTC GCCCAACCAC
    TCCCTTCACA ACGAGGCGGC AGATGACTCC CAGCTTGAAA AGGCCAACCT CATAGAGCTC
    GAAGATGACC CCCACAGTGG AAGACGGGGA GTCCCGCACA GCCTGAGTGG CTTGCCAGAC
    CCCCTCGTAG CCCGGATGTC CATTTGCTCC GAGGACAAGA AGAGCCCTTC TGAATGCAGC
    TTGATAGCCA GCAGCCCCGA GGAAAACTGG CCAGCATGCC ACAAATCCTA CAACCTGAAT
    CGCACCCCCA GCACCGTGAC CCTGAACAAC AACAGTGCCC CGGCTAACAG AGCCAACCAG
    AATTCCGATG AGACGGAGGG GCTGAGGGAG ACGCCTCAAG TCCTCCTGAG GTCAGGCTCC
    AGTTCCCACT CCACCGCTCT GCAGAATGAG AACCTGAAGA GCATGGCACA CAAGCGAGGC
    CAGCGTTCCA GCTACACGAG GCTCTCAAAG GACTCCTCAG AGCTCCACGC AGTAGCCTCC
    TCCGATAGTA CAGGTTTTGG AGAAGAAAGG GAGAGCATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53364
    Clone ID Related Accession (Same CDS sequence) XM_015465439.1 , XM_010810306.2
    Accession Version XM_015465439.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGAAGTTATC
    AAGGAGGATG CTGCTGAGGG CCTTTCTTCA CCCACAGACT CCTCGAGGGA GCAAACTTGG
    ACGAGAAAGC TAAAGATGCC ATGGCCGTGT GACTCTGGGT TTAACAAACA GAGACAGGGA
    TCAGGCCCAG CCTCAGGCAC AATACCACTG AATTCGATGA ACGTGGATGC AGTTTGTGAG
    AAACTGAAAC AAATTGAAGG GCTGGACCAG AGCATGCTGC CTCAGTACTG TGCAACCATC
    AAAAAGGCGA ACATAAATGG CCGTGTGTTG GCTCAGTGTA ACATTGATGA ACTGAAGAAG
    GAAATGAATA TGAATTTTGG AGACTGGCAT CTTTTTAGAA GCACAATATT AGAAATGAGA
    AATGCAGAAA ACCAAGTGGT CCCTGAAGAC CCACGTTTAC TGAGTGAAAA CAGCAGCTCT
    GTTCCTCACG GTGAACCTGC TCGTCGCTCC GCACACAGCG AGCTGCCCCA CACCGAGCTC
    TCCAGTCAGG CTCCCTACAC CCTGAACTTC AGCTTTGAAG AGCTGAACAC GCTCGGCCTG
    GACGAAGGGG CTCCTCGCCA CAGCAACCTG AGTTGGCAGT CACAAACTCG CAGAACCCCA
    AGTCTTTCCA GTCTCACTTC CCAGGACTCC AGTATTGAAA TTTCAAAGCT TACTGATAAG
    GTGCAGGCTG AGTATCGAGA CGCTTATAGA GAGTACATTG CTCAGATGTC TCAGTTAGAA
    GGGGGCACAG GTTCCACAAC CATGAGTGGC AGGTCTTCTC CACACAGCAC GTATTATATG
    GGTCAGAGTT CATCCGGGGG CTCCATTCAC TCTAGTCTAG AACCCGAGAA AGGGAAGGAT
    AGTGAACCAA AGCAAGATGA TACACGGAAG TCATTTCTGA TGAAGAGGGG AGATGTTATA
    GATTATTCCT CATCAGGAGT TTCCACCAAT GATGCCTCAC CCCTGGATCC TATTACCGAG
    GAAGATGAAA AATCTGATCA GTCAGGCAGT AAGCTTCTCC CAGGCAAGAA GTCCTCAGAA
    AGGTCAAGTC TCTTCCAGAC AGACCTGAAG CTGAAGGGAG GTGGACTGCG CTATCAGAAA
    CTCCCGAGTG ACGAAGACGA GTCTGGGACA GAAGAGTCAG ACAACACTCC ACTGCTCAAA
    GACGACAAAG ACAAGAAAGC TGAAGGGAAA GCAGAGAGAG CATTGAAGTC TCCAGAGCAC
    AGTGCAGAGC CCATTCGAAC CTTCATCAAG GCCAAAGAGT ACTTGTCAGA TGCCCTCCTT
    GACAAAAAGG ACTCCTCTGA CTCAGGAGTG AGGTCCAATG AGAGCTCGCC CAACCACTCC
    CTTCACAACG AGGCGGCAGA TGACTCCCAG CTTGAAAAGG CCAACCTCAT AGAGCTCGAA
    GATGACCCCC ACAGTGGAAG ACGGGGAGTC CCGCACAGCC TGAGTGGCTT GCCAGACCCC
    CTCGTAGCCC GGATGTCCAT TTGCTCCGAG GACAAGAAGA GCCCTTCTGA ATGCAGCTTG
    ATAGCCAGCA GCCCCGAGGA AAACTGGCCA GCATGCCACA AATCCTACAA CCTGAATCGC
    ACCCCCAGCA CCGTGACCCT GAACAACAAC AGTGCCCCGG CTAACAGAGC CAACCAGAAT
    TCCGATGAGA CGGAGGGGCT GAGGGAGACG CCTCAAGTCC TCCTGAGGTC AGGCTCCAGT
    TCCCACTCCA CCGCTCTGCA GAATGAGAAC CTGAAGAGCA TGGCACACAA GCGAGGCCAG
    CGTTCCAGCT ACACGAGGCT CTCAAAGGAC TCCTCAGAGC TCCACGCAGT AGCCTCCTCC
    GATAGTACAG GTTTTGGAGA AGAAAGGGAG AGCATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320925.1
    CDS207..5588
    Translation

    Target ORF information:

    RefSeq Version XM_015465439.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465439.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGAAGTTATC
    AAGGAGGATG CTGCTGAGGG CCTTTCTTCA CCCACAGACT CCTCGAGGGA GCAAACTTGG
    ACGAGAAAGC TAAAGATGCC ATGGCCGTGT GACTCTGGGT TTAACAAACA GAGACAGGGA
    TCAGGCCCAG CCTCAGGCAC AATACCACTG AATTCGATGA ACGTGGATGC AGTTTGTGAG
    AAACTGAAAC AAATTGAAGG GCTGGACCAG AGCATGCTGC CTCAGTACTG TGCAACCATC
    AAAAAGGCGA ACATAAATGG CCGTGTGTTG GCTCAGTGTA ACATTGATGA ACTGAAGAAG
    GAAATGAATA TGAATTTTGG AGACTGGCAT CTTTTTAGAA GCACAATATT AGAAATGAGA
    AATGCAGAAA ACCAAGTGGT CCCTGAAGAC CCACGTTTAC TGAGTGAAAA CAGCAGCTCT
    GTTCCTCACG GTGAACCTGC TCGTCGCTCC GCACACAGCG AGCTGCCCCA CACCGAGCTC
    TCCAGTCAGG CTCCCTACAC CCTGAACTTC AGCTTTGAAG AGCTGAACAC GCTCGGCCTG
    GACGAAGGGG CTCCTCGCCA CAGCAACCTG AGTTGGCAGT CACAAACTCG CAGAACCCCA
    AGTCTTTCCA GTCTCACTTC CCAGGACTCC AGTATTGAAA TTTCAAAGCT TACTGATAAG
    GTGCAGGCTG AGTATCGAGA CGCTTATAGA GAGTACATTG CTCAGATGTC TCAGTTAGAA
    GGGGGCACAG GTTCCACAAC CATGAGTGGC AGGTCTTCTC CACACAGCAC GTATTATATG
    GGTCAGAGTT CATCCGGGGG CTCCATTCAC TCTAGTCTAG AACCCGAGAA AGGGAAGGAT
    AGTGAACCAA AGCAAGATGA TACACGGAAG TCATTTCTGA TGAAGAGGGG AGATGTTATA
    GATTATTCCT CATCAGGAGT TTCCACCAAT GATGCCTCAC CCCTGGATCC TATTACCGAG
    GAAGATGAAA AATCTGATCA GTCAGGCAGT AAGCTTCTCC CAGGCAAGAA GTCCTCAGAA
    AGGTCAAGTC TCTTCCAGAC AGACCTGAAG CTGAAGGGAG GTGGACTGCG CTATCAGAAA
    CTCCCGAGTG ACGAAGACGA GTCTGGGACA GAAGAGTCAG ACAACACTCC ACTGCTCAAA
    GACGACAAAG ACAAGAAAGC TGAAGGGAAA GCAGAGAGAG CATTGAAGTC TCCAGAGCAC
    AGTGCAGAGC CCATTCGAAC CTTCATCAAG GCCAAAGAGT ACTTGTCAGA TGCCCTCCTT
    GACAAAAAGG ACTCCTCTGA CTCAGGAGTG AGGTCCAATG AGAGCTCGCC CAACCACTCC
    CTTCACAACG AGGCGGCAGA TGACTCCCAG CTTGAAAAGG CCAACCTCAT AGAGCTCGAA
    GATGACCCCC ACAGTGGAAG ACGGGGAGTC CCGCACAGCC TGAGTGGCTT GCCAGACCCC
    CTCGTAGCCC GGATGTCCAT TTGCTCCGAG GACAAGAAGA GCCCTTCTGA ATGCAGCTTG
    ATAGCCAGCA GCCCCGAGGA AAACTGGCCA GCATGCCACA AATCCTACAA CCTGAATCGC
    ACCCCCAGCA CCGTGACCCT GAACAACAAC AGTGCCCCGG CTAACAGAGC CAACCAGAAT
    TCCGATGAGA CGGAGGGGCT GAGGGAGACG CCTCAAGTCC TCCTGAGGTC AGGCTCCAGT
    TCCCACTCCA CCGCTCTGCA GAATGAGAAC CTGAAGAGCA TGGCACACAA GCGAGGCCAG
    CGTTCCAGCT ACACGAGGCT CTCAAAGGAC TCCTCAGAGC TCCACGCAGT AGCCTCCTCC
    GATAGTACAG GTTTTGGAGA AGAAAGGGAG AGCATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53364
    Clone ID Related Accession (Same CDS sequence) XM_015465439.1 , XM_010810306.2
    Accession Version XM_010810306.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810306.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGAAGTTATC
    AAGGAGGATG CTGCTGAGGG CCTTTCTTCA CCCACAGACT CCTCGAGGGA GCAAACTTGG
    ACGAGAAAGC TAAAGATGCC ATGGCCGTGT GACTCTGGGT TTAACAAACA GAGACAGGGA
    TCAGGCCCAG CCTCAGGCAC AATACCACTG AATTCGATGA ACGTGGATGC AGTTTGTGAG
    AAACTGAAAC AAATTGAAGG GCTGGACCAG AGCATGCTGC CTCAGTACTG TGCAACCATC
    AAAAAGGCGA ACATAAATGG CCGTGTGTTG GCTCAGTGTA ACATTGATGA ACTGAAGAAG
    GAAATGAATA TGAATTTTGG AGACTGGCAT CTTTTTAGAA GCACAATATT AGAAATGAGA
    AATGCAGAAA ACCAAGTGGT CCCTGAAGAC CCACGTTTAC TGAGTGAAAA CAGCAGCTCT
    GTTCCTCACG GTGAACCTGC TCGTCGCTCC GCACACAGCG AGCTGCCCCA CACCGAGCTC
    TCCAGTCAGG CTCCCTACAC CCTGAACTTC AGCTTTGAAG AGCTGAACAC GCTCGGCCTG
    GACGAAGGGG CTCCTCGCCA CAGCAACCTG AGTTGGCAGT CACAAACTCG CAGAACCCCA
    AGTCTTTCCA GTCTCACTTC CCAGGACTCC AGTATTGAAA TTTCAAAGCT TACTGATAAG
    GTGCAGGCTG AGTATCGAGA CGCTTATAGA GAGTACATTG CTCAGATGTC TCAGTTAGAA
    GGGGGCACAG GTTCCACAAC CATGAGTGGC AGGTCTTCTC CACACAGCAC GTATTATATG
    GGTCAGAGTT CATCCGGGGG CTCCATTCAC TCTAGTCTAG AACCCGAGAA AGGGAAGGAT
    AGTGAACCAA AGCAAGATGA TACACGGAAG TCATTTCTGA TGAAGAGGGG AGATGTTATA
    GATTATTCCT CATCAGGAGT TTCCACCAAT GATGCCTCAC CCCTGGATCC TATTACCGAG
    GAAGATGAAA AATCTGATCA GTCAGGCAGT AAGCTTCTCC CAGGCAAGAA GTCCTCAGAA
    AGGTCAAGTC TCTTCCAGAC AGACCTGAAG CTGAAGGGAG GTGGACTGCG CTATCAGAAA
    CTCCCGAGTG ACGAAGACGA GTCTGGGACA GAAGAGTCAG ACAACACTCC ACTGCTCAAA
    GACGACAAAG ACAAGAAAGC TGAAGGGAAA GCAGAGAGAG CATTGAAGTC TCCAGAGCAC
    AGTGCAGAGC CCATTCGAAC CTTCATCAAG GCCAAAGAGT ACTTGTCAGA TGCCCTCCTT
    GACAAAAAGG ACTCCTCTGA CTCAGGAGTG AGGTCCAATG AGAGCTCGCC CAACCACTCC
    CTTCACAACG AGGCGGCAGA TGACTCCCAG CTTGAAAAGG CCAACCTCAT AGAGCTCGAA
    GATGACCCCC ACAGTGGAAG ACGGGGAGTC CCGCACAGCC TGAGTGGCTT GCCAGACCCC
    CTCGTAGCCC GGATGTCCAT TTGCTCCGAG GACAAGAAGA GCCCTTCTGA ATGCAGCTTG
    ATAGCCAGCA GCCCCGAGGA AAACTGGCCA GCATGCCACA AATCCTACAA CCTGAATCGC
    ACCCCCAGCA CCGTGACCCT GAACAACAAC AGTGCCCCGG CTAACAGAGC CAACCAGAAT
    TCCGATGAGA CGGAGGGGCT GAGGGAGACG CCTCAAGTCC TCCTGAGGTC AGGCTCCAGT
    TCCCACTCCA CCGCTCTGCA GAATGAGAAC CTGAAGAGCA TGGCACACAA GCGAGGCCAG
    CGTTCCAGCT ACACGAGGCT CTCAAAGGAC TCCTCAGAGC TCCACGCAGT AGCCTCCTCC
    GATAGTACAG GTTTTGGAGA AGAAAGGGAG AGCATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808608.1
    CDS207..5588
    Translation

    Target ORF information:

    RefSeq Version XM_010810306.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810306.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGTACAG TGTCTACCCA
    ATCATTTGGG CAGCAGGCAG AGGCCATGCT GATATAGTGC AACTTTTACT GCAAAATGGT
    GCCAAGGTCA ACTGCTCTGA CAAGTATGGA ACCACCCCTT TGGTTTGGGC TGCACGAAAA
    GGTCATTTGG AATGTGTAAA ACATTTATTA GCCATGGGAG CTGATGTTGA TCAAGAAGGA
    GCTAATTCAA TGACCGCACT TATTGTGGCA GTAAAAGGAG GCTACACACA GTCAGTGAAA
    GAAATTTTGA AGCGAAATCC AAATGTAAAT TTAACAGATA AGGATGGAAA TACAGCCTTG
    ATGATTGCAT CGAAGGAGGG ACATACAGAA ATTGTACAGG ATCTCCTTGA CGCTGGGACA
    TATGTGAACA TACCTGATAG GAGTGGAGAT ACTGTCTTAA TTGGTGCTGT CAGAGGTGGT
    CATGTGGAAA TTGTTCGAGC ACTTCTCCAA AAATATGCTG ATATCGACAT TAGAGGACAG
    GACAGTAAAA CTGCCTTGTA TTGGGCTGTT GAGAAAGGAA ATGCAACAAT GGTGAGAGAC
    ATCTTACAGT GCAATCCTGA CACTGAAATA TGCACGAAGG ATGGCGAAAC ACCCCTTATA
    AAGGCTACCA AGATGAGAAA CATAGAAGTG GTAGAGCTGC TGCTGGATAA AGGAGCCAAA
    GTGTCTGCTG TCGATAAGAA AGGAGACACC CCTCTGCACA TTGCTATTCG TGGGAGGAGT
    CGGAAACTGG CAGAACTCCT TTTAAGAAAC CCCAAAGATG GACGGTTACT GTATAGACCC
    AACAAAGCAG GCGAGACTCC CTACAATATT GACTGTAGCC ATCAGAAAAG TATTTTAACT
    CAAATATTTG GAGCCAGACA CTTGTCTCCT ACCGAGACAG ATGGTGACAT GCTTGGTTAC
    GATCTGTACA GCAGTGCCCT GGCAGATATT CTCAGTGAGC CTACCATGCA GCCGCCCATT
    TGTGTGGGCC TGTATGCACA GTGGGGAAGT GGTAAATCCT TCTTACTCAA GAAACTAGAA
    GATGAAATGA AAACTTTTGC AGGACAACAG ATTGAACCTC TGTTTCAGTT TTCATGGCTT
    GTGGTGTTTC TGACCCTGCT CCTTTGTGGA GGGCTTGGTT TATTGTTTGC TTTTACAGTA
    GACCTAAATC TTGGAATTGC AGTGTCATTG AGCTTCTTGG CTGTTTTGTA TATATTTTTT
    ATTGTCATTT ACTTTGGTGG ACGGAGGGAA GGAGAGAGTT GGAATTGGGC CTGGGTCCTC
    AGTACTAGAT TGGCAAGACA TATCGGCTAT TTGGAGCTCC TCCTCAAATT GATGTTTGTG
    AACCCACCTG AGCTGCCAGA GCAAACCACT AAAGCTCTAC CTGTGAGGTT TTTGTTTACA
    GATTATAATA GACTGTCCAG CGTAGGTGGG GAAACTTCAA TGGCTGAAAT GATCGCCACC
    CTCTCGGATG CTTGTGAAAG AGAGTTTGGC TTTTTGGCAA CCAGGCTTTT CCGAGTGTTC
    AAGACTGAAG ATACGCAGGG TAAAAAGAAA TGGAAAAAAA CGTGTTGTCT CCCATCTTTT
    GTCATCTTCC TTTTCATCTT TGGCTGCATT ATTGCTGCGA TTACTCTTCT GGCTATATTC
    AGAGTTGACC CGAAGCATAT GACAGTGAAT GCTGTCCTCA TATCAATTGC GTCCATAGTG
    GGGTTGGCGT TTGTGCTGAA CTGTCGAACA TGGTGGCAGG TGCTGGACTC ACTTCTGAAT
    TCTCAAAGGA AACGCCTCCA TAATGCTGCC TCCAAACTGC ACAAACTGAA AAGTGAAGGA
    TTCATGAAAG TTCTTAAGTG TGAAGTGGAA TTGATGGCCA GGATGGCAAA AACCATTGAC
    AGCTTCACTC AGAATCAGAC AAGGCTGGTG GTTATCATCG ATGGGTTAGA TGCCTGTGAG
    CAGGACAAAG TACTCCAGAT GCTGGACACT GTCCGAGTTC TGTTTTCAAA AGGCCCATTC
    ATTGCCATTT TTGCAAGTGA TCCACATATT ATCATAAAGG CAATTAATCA GAACCTCAAT
    AGTGTGCTTC GTGACTCAAA CATCAATGGA CATGACTATA TGCGCAATAT AGTTCATCTG
    CCCGTTTTCC TCAACAGCCG GGGACTAAGC AGTGCAAGAA AATTTCTTGT AACTTCAACA
    ACTAATGGAG ATGTTCCATG CTCGGATAAT ACAGGGATGC AGGAAGATGC TGACAGAAGA
    GTTTCACAAA ACAGCCTTGG AGAGATGACA AAACTTGGTA GCAAGACGGC CCTCAATAGA
    CGGGACACTT ACCGAAGAAG GCAGATGCAG CGCACGATTA CCCGGCAGAT GTCCTTCGAC
    CTCACGAAAC TGCTGGTTAC GGAGGACTGG TTCAGTGACA TAAGCCCGCA GACCATGAGG
    AGACTGCTCA ACATTGTTTC TGTGACAGGA CGATTACTGA GAGCCAACCA GATAAGTTTC
    AACTGGGATA GGCTTGCTAG CTGGATCAAC CTAACTGAGC AGTGGCCGTA CAGAACTTCA
    TGGCTCATAT TGTATTTGGA AGAGACCGAA GGTATCCCAG ATCAAATGAC ATTAAAAACC
    ATCTATGAAA GAATATCAAA GAATATTCCA ACAACTAAAG ATGTTGAGCC ACTGCTTGAA
    ATAGATGGAG ATATAAGAAA TTTTGAAGTG TTTTTGTCTT CAAGGACCCC AGTTCTTGTG
    GCTCGAGATG TGAAAACCTT TTTGCCATGC ACTGTAAATC TGGACCCCAA ACTGCGGGAG
    ATTATTGCAG ATGTTCGTGC TGCAAGAGAC CAGATTAATA TCGGAGGACT TGCGTATCCC
    CCGCTACCTC TGCACGAGCC TCCCCCTCGA CCGCCGTCCG GCTACAGTCA GCCAGCGTCT
    GTCTGCTCTT CGTCCACCTC CTTCAATGGG CCATTCGGGG GAGGGGTTGT GTCGCCGCAG
    CCTCATAGCA GCTACTACAG CGGCATGACA GGACCCCAGC ACCCCTTCTA CAACAGGCCA
    TTCTTTGCCC CCTACCTTTA CACGCCAAGG TACTGCCCTG GCGGCTCCCA CCATCTCATC
    TCACGTCCAT CAGTAAAAAC GAATTTGCCC AGAGATCAGA GCAATGGCCT AGAAGTTATC
    AAGGAGGATG CTGCTGAGGG CCTTTCTTCA CCCACAGACT CCTCGAGGGA GCAAACTTGG
    ACGAGAAAGC TAAAGATGCC ATGGCCGTGT GACTCTGGGT TTAACAAACA GAGACAGGGA
    TCAGGCCCAG CCTCAGGCAC AATACCACTG AATTCGATGA ACGTGGATGC AGTTTGTGAG
    AAACTGAAAC AAATTGAAGG GCTGGACCAG AGCATGCTGC CTCAGTACTG TGCAACCATC
    AAAAAGGCGA ACATAAATGG CCGTGTGTTG GCTCAGTGTA ACATTGATGA ACTGAAGAAG
    GAAATGAATA TGAATTTTGG AGACTGGCAT CTTTTTAGAA GCACAATATT AGAAATGAGA
    AATGCAGAAA ACCAAGTGGT CCCTGAAGAC CCACGTTTAC TGAGTGAAAA CAGCAGCTCT
    GTTCCTCACG GTGAACCTGC TCGTCGCTCC GCACACAGCG AGCTGCCCCA CACCGAGCTC
    TCCAGTCAGG CTCCCTACAC CCTGAACTTC AGCTTTGAAG AGCTGAACAC GCTCGGCCTG
    GACGAAGGGG CTCCTCGCCA CAGCAACCTG AGTTGGCAGT CACAAACTCG CAGAACCCCA
    AGTCTTTCCA GTCTCACTTC CCAGGACTCC AGTATTGAAA TTTCAAAGCT TACTGATAAG
    GTGCAGGCTG AGTATCGAGA CGCTTATAGA GAGTACATTG CTCAGATGTC TCAGTTAGAA
    GGGGGCACAG GTTCCACAAC CATGAGTGGC AGGTCTTCTC CACACAGCAC GTATTATATG
    GGTCAGAGTT CATCCGGGGG CTCCATTCAC TCTAGTCTAG AACCCGAGAA AGGGAAGGAT
    AGTGAACCAA AGCAAGATGA TACACGGAAG TCATTTCTGA TGAAGAGGGG AGATGTTATA
    GATTATTCCT CATCAGGAGT TTCCACCAAT GATGCCTCAC CCCTGGATCC TATTACCGAG
    GAAGATGAAA AATCTGATCA GTCAGGCAGT AAGCTTCTCC CAGGCAAGAA GTCCTCAGAA
    AGGTCAAGTC TCTTCCAGAC AGACCTGAAG CTGAAGGGAG GTGGACTGCG CTATCAGAAA
    CTCCCGAGTG ACGAAGACGA GTCTGGGACA GAAGAGTCAG ACAACACTCC ACTGCTCAAA
    GACGACAAAG ACAAGAAAGC TGAAGGGAAA GCAGAGAGAG CATTGAAGTC TCCAGAGCAC
    AGTGCAGAGC CCATTCGAAC CTTCATCAAG GCCAAAGAGT ACTTGTCAGA TGCCCTCCTT
    GACAAAAAGG ACTCCTCTGA CTCAGGAGTG AGGTCCAATG AGAGCTCGCC CAACCACTCC
    CTTCACAACG AGGCGGCAGA TGACTCCCAG CTTGAAAAGG CCAACCTCAT AGAGCTCGAA
    GATGACCCCC ACAGTGGAAG ACGGGGAGTC CCGCACAGCC TGAGTGGCTT GCCAGACCCC
    CTCGTAGCCC GGATGTCCAT TTGCTCCGAG GACAAGAAGA GCCCTTCTGA ATGCAGCTTG
    ATAGCCAGCA GCCCCGAGGA AAACTGGCCA GCATGCCACA AATCCTACAA CCTGAATCGC
    ACCCCCAGCA CCGTGACCCT GAACAACAAC AGTGCCCCGG CTAACAGAGC CAACCAGAAT
    TCCGATGAGA CGGAGGGGCT GAGGGAGACG CCTCAAGTCC TCCTGAGGTC AGGCTCCAGT
    TCCCACTCCA CCGCTCTGCA GAATGAGAAC CTGAAGAGCA TGGCACACAA GCGAGGCCAG
    CGTTCCAGCT ACACGAGGCT CTCAAAGGAC TCCTCAGAGC TCCACGCAGT AGCCTCCTCC
    GATAGTACAG GTTTTGGAGA AGAAAGGGAG AGCATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53365
    Clone ID Related Accession (Same CDS sequence) XM_015465442.1 , XM_010810307.3 , XM_010810307.2
    Accession Version XM_015465442.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTACCGTGT
    GACTCTGGGT TTAACAAACA GAGACAGGGA TCAGGCCCAG CCTCAGGCAC AATACCACTG
    AATTCGATGA ACGTGGATGC AGTTTGTGAG AAACTGAAAC AAATTGAAGG GCTGGACCAG
    AGCATGCTGC CTCAGTACTG TGCAACCATC AAAAAGGCGA ACATAAATGG CCGTGTGTTG
    GCTCAGTGTA ACATTGATGA ACTGAAGAAG GAAATGAATA TGAATTTTGG AGACTGGCAT
    CTTTTTAGAA GCACAATATT AGAAATGAGA AATGCAGAAA ACCAAGTGGT CCCTGAAGAC
    CCACGTTTAC TGAGTGAAAA CAGCAGCTCT GTTCCTCACG GTGAACCTGC TCGTCGCTCC
    GCACACAGCG AGCTGCCCCA CACCGAGCTC TCCAGTCAGG CTCCCTACAC CCTGAACTTC
    AGCTTTGAAG AGCTGAACAC GCTCGGCCTG GACGAAGGGG CTCCTCGCCA CAGCAACCTG
    AGTTGGCAGT CACAAACTCG CAGAACCCCA AGTCTTTCCA GTCTCACTTC CCAGGACTCC
    AGTATTGAAA TTTCAAAGCT TACTGATAAG GTGCAGGCTG AGTATCGAGA CGCTTATAGA
    GAGTACATTG CTCAGATGTC TCAGTTAGAA GGGGGCACAG GTTCCACAAC CATGAGTGGC
    AGGTCTTCTC CACACAGCAC GTATTATATG GGTCAGAGTT CATCCGGGGG CTCCATTCAC
    TCTAGTCTAG AACCCGAGAA AGGGAAGGAT AGTGAACCAA AGCAAGATGA TACACGGAAG
    TCATTTCTGA TGAAGAGGGG AGATGTTATA GATTATTCCT CATCAGGAGT TTCCACCAAT
    GATGCCTCAC CCCTGGATCC TATTACCGAG GAAGATGAAA AATCTGATCA GTCAGGCAGT
    AAGCTTCTCC CAGGCAAGAA GTCCTCAGAA AGGTCAAGTC TCTTCCAGAC AGACCTGAAG
    CTGAAGGGAG GTGGACTGCG CTATCAGAAA CTCCCGAGTG ACGAAGACGA GTCTGGGACA
    GAAGAGTCAG ACAACACTCC ACTGCTCAAA GACGACAAAG ACAAGAAAGC TGAAGGGAAA
    GCAGAGAGAG CATTGAAGTC TCCAGAGCAC AGTGCAGAGC CCATTCGAAC CTTCATCAAG
    GCCAAAGAGT ACTTGTCAGA TGCCCTCCTT GACAAAAAGG ACTCCTCTGA CTCAGGAGTG
    AGGTCCAATG AGAGCTCGCC CAACCACTCC CTTCACAACG AGGCGGCAGA TGACTCCCAG
    CTTGAAAAGG CCAACCTCAT AGAGCTCGAA GATGACCCCC ACAGTGGAAG ACGGGGAGTC
    CCGCACAGCC TGAGTGGCTT GCCAGACCCC CTCGTAGCCC GGATGTCCAT TTGCTCCGAG
    GACAAGAAGA GCCCTTCTGA ATGCAGCTTG ATAGCCAGCA GCCCCGAGGA AAACTGGCCA
    GCATGCCACA AATCCTACAA CCTGAATCGC ACCCCCAGCA CCGTGACCCT GAACAACAAC
    AGTGCCCCGG CTAACAGAGC CAACCAGAAT TCCGATGAGA CGGAGGGGCT GAGGGAGACG
    CCTCAAGTCC TCCTGAGGTC AGGCTCCAGT TCCCACTCCA CCGCTCTGCA GAATGAGAAC
    CTGAAGAGCA TGGCACACAA GCGAGGCCAG CGTTCCAGCT ACACGAGGCT CTCAAAGGAC
    TCCTCAGAGC TCCACGCAGT AGCCTCCTCC GATAGTACAG GTTTTGGAGA AGAAAGGGAG
    AGCATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320928.1
    CDS207..5498
    Translation

    Target ORF information:

    RefSeq Version XM_015465442.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465442.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTACCGTGT
    GACTCTGGGT TTAACAAACA GAGACAGGGA TCAGGCCCAG CCTCAGGCAC AATACCACTG
    AATTCGATGA ACGTGGATGC AGTTTGTGAG AAACTGAAAC AAATTGAAGG GCTGGACCAG
    AGCATGCTGC CTCAGTACTG TGCAACCATC AAAAAGGCGA ACATAAATGG CCGTGTGTTG
    GCTCAGTGTA ACATTGATGA ACTGAAGAAG GAAATGAATA TGAATTTTGG AGACTGGCAT
    CTTTTTAGAA GCACAATATT AGAAATGAGA AATGCAGAAA ACCAAGTGGT CCCTGAAGAC
    CCACGTTTAC TGAGTGAAAA CAGCAGCTCT GTTCCTCACG GTGAACCTGC TCGTCGCTCC
    GCACACAGCG AGCTGCCCCA CACCGAGCTC TCCAGTCAGG CTCCCTACAC CCTGAACTTC
    AGCTTTGAAG AGCTGAACAC GCTCGGCCTG GACGAAGGGG CTCCTCGCCA CAGCAACCTG
    AGTTGGCAGT CACAAACTCG CAGAACCCCA AGTCTTTCCA GTCTCACTTC CCAGGACTCC
    AGTATTGAAA TTTCAAAGCT TACTGATAAG GTGCAGGCTG AGTATCGAGA CGCTTATAGA
    GAGTACATTG CTCAGATGTC TCAGTTAGAA GGGGGCACAG GTTCCACAAC CATGAGTGGC
    AGGTCTTCTC CACACAGCAC GTATTATATG GGTCAGAGTT CATCCGGGGG CTCCATTCAC
    TCTAGTCTAG AACCCGAGAA AGGGAAGGAT AGTGAACCAA AGCAAGATGA TACACGGAAG
    TCATTTCTGA TGAAGAGGGG AGATGTTATA GATTATTCCT CATCAGGAGT TTCCACCAAT
    GATGCCTCAC CCCTGGATCC TATTACCGAG GAAGATGAAA AATCTGATCA GTCAGGCAGT
    AAGCTTCTCC CAGGCAAGAA GTCCTCAGAA AGGTCAAGTC TCTTCCAGAC AGACCTGAAG
    CTGAAGGGAG GTGGACTGCG CTATCAGAAA CTCCCGAGTG ACGAAGACGA GTCTGGGACA
    GAAGAGTCAG ACAACACTCC ACTGCTCAAA GACGACAAAG ACAAGAAAGC TGAAGGGAAA
    GCAGAGAGAG CATTGAAGTC TCCAGAGCAC AGTGCAGAGC CCATTCGAAC CTTCATCAAG
    GCCAAAGAGT ACTTGTCAGA TGCCCTCCTT GACAAAAAGG ACTCCTCTGA CTCAGGAGTG
    AGGTCCAATG AGAGCTCGCC CAACCACTCC CTTCACAACG AGGCGGCAGA TGACTCCCAG
    CTTGAAAAGG CCAACCTCAT AGAGCTCGAA GATGACCCCC ACAGTGGAAG ACGGGGAGTC
    CCGCACAGCC TGAGTGGCTT GCCAGACCCC CTCGTAGCCC GGATGTCCAT TTGCTCCGAG
    GACAAGAAGA GCCCTTCTGA ATGCAGCTTG ATAGCCAGCA GCCCCGAGGA AAACTGGCCA
    GCATGCCACA AATCCTACAA CCTGAATCGC ACCCCCAGCA CCGTGACCCT GAACAACAAC
    AGTGCCCCGG CTAACAGAGC CAACCAGAAT TCCGATGAGA CGGAGGGGCT GAGGGAGACG
    CCTCAAGTCC TCCTGAGGTC AGGCTCCAGT TCCCACTCCA CCGCTCTGCA GAATGAGAAC
    CTGAAGAGCA TGGCACACAA GCGAGGCCAG CGTTCCAGCT ACACGAGGCT CTCAAAGGAC
    TCCTCAGAGC TCCACGCAGT AGCCTCCTCC GATAGTACAG GTTTTGGAGA AGAAAGGGAG
    AGCATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53365
    Clone ID Related Accession (Same CDS sequence) XM_015465442.1 , XM_010810307.3 , XM_010810307.2
    Accession Version XM_010810307.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810307.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTACCGTGT
    GACTCTGGGT TTAACAAACA GAGACAGGGA TCAGGCCCAG CCTCAGGCAC AATACCACTG
    AATTCGATGA ACGTGGATGC AGTTTGTGAG AAACTGAAAC AAATTGAAGG GCTGGACCAG
    AGCATGCTGC CTCAGTACTG TGCAACCATC AAAAAGGCGA ACATAAATGG CCGTGTGTTG
    GCTCAGTGTA ACATTGATGA ACTGAAGAAG GAAATGAATA TGAATTTTGG AGACTGGCAT
    CTTTTTAGAA GCACAATATT AGAAATGAGA AATGCAGAAA ACCAAGTGGT CCCTGAAGAC
    CCACGTTTAC TGAGTGAAAA CAGCAGCTCT GTTCCTCACG GTGAACCTGC TCGTCGCTCC
    GCACACAGCG AGCTGCCCCA CACCGAGCTC TCCAGTCAGG CTCCCTACAC CCTGAACTTC
    AGCTTTGAAG AGCTGAACAC GCTCGGCCTG GACGAAGGGG CTCCTCGCCA CAGCAACCTG
    AGTTGGCAGT CACAAACTCG CAGAACCCCA AGTCTTTCCA GTCTCACTTC CCAGGACTCC
    AGTATTGAAA TTTCAAAGCT TACTGATAAG GTGCAGGCTG AGTATCGAGA CGCTTATAGA
    GAGTACATTG CTCAGATGTC TCAGTTAGAA GGGGGCACAG GTTCCACAAC CATGAGTGGC
    AGGTCTTCTC CACACAGCAC GTATTATATG GGTCAGAGTT CATCCGGGGG CTCCATTCAC
    TCTAGTCTAG AACCCGAGAA AGGGAAGGAT AGTGAACCAA AGCAAGATGA TACACGGAAG
    TCATTTCTGA TGAAGAGGGG AGATGTTATA GATTATTCCT CATCAGGAGT TTCCACCAAT
    GATGCCTCAC CCCTGGATCC TATTACCGAG GAAGATGAAA AATCTGATCA GTCAGGCAGT
    AAGCTTCTCC CAGGCAAGAA GTCCTCAGAA AGGTCAAGTC TCTTCCAGAC AGACCTGAAG
    CTGAAGGGAG GTGGACTGCG CTATCAGAAA CTCCCGAGTG ACGAAGACGA GTCTGGGACA
    GAAGAGTCAG ACAACACTCC ACTGCTCAAA GACGACAAAG ACAAGAAAGC TGAAGGGAAA
    GCAGAGAGAG CATTGAAGTC TCCAGAGCAC AGTGCAGAGC CCATTCGAAC CTTCATCAAG
    GCCAAAGAGT ACTTGTCAGA TGCCCTCCTT GACAAAAAGG ACTCCTCTGA CTCAGGAGTG
    AGGTCCAATG AGAGCTCGCC CAACCACTCC CTTCACAACG AGGCGGCAGA TGACTCCCAG
    CTTGAAAAGG CCAACCTCAT AGAGCTCGAA GATGACCCCC ACAGTGGAAG ACGGGGAGTC
    CCGCACAGCC TGAGTGGCTT GCCAGACCCC CTCGTAGCCC GGATGTCCAT TTGCTCCGAG
    GACAAGAAGA GCCCTTCTGA ATGCAGCTTG ATAGCCAGCA GCCCCGAGGA AAACTGGCCA
    GCATGCCACA AATCCTACAA CCTGAATCGC ACCCCCAGCA CCGTGACCCT GAACAACAAC
    AGTGCCCCGG CTAACAGAGC CAACCAGAAT TCCGATGAGA CGGAGGGGCT GAGGGAGACG
    CCTCAAGTCC TCCTGAGGTC AGGCTCCAGT TCCCACTCCA CCGCTCTGCA GAATGAGAAC
    CTGAAGAGCA TGGCACACAA GCGAGGCCAG CGTTCCAGCT ACACGAGGCT CTCAAAGGAC
    TCCTCAGAGC TCCACGCAGT AGCCTCCTCC GATAGTACAG GTTTTGGAGA AGAAAGGGAG
    AGCATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808609.1
    CDS213..5504
    Translation

    Target ORF information:

    RefSeq Version XM_010810307.3
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D interacting substrate 220 (KIDINS220), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810307.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTACCGTGT
    GACTCTGGGT TTAACAAACA GAGACAGGGA TCAGGCCCAG CCTCAGGCAC AATACCACTG
    AATTCGATGA ACGTGGATGC AGTTTGTGAG AAACTGAAAC AAATTGAAGG GCTGGACCAG
    AGCATGCTGC CTCAGTACTG TGCAACCATC AAAAAGGCGA ACATAAATGG CCGTGTGTTG
    GCTCAGTGTA ACATTGATGA ACTGAAGAAG GAAATGAATA TGAATTTTGG AGACTGGCAT
    CTTTTTAGAA GCACAATATT AGAAATGAGA AATGCAGAAA ACCAAGTGGT CCCTGAAGAC
    CCACGTTTAC TGAGTGAAAA CAGCAGCTCT GTTCCTCACG GTGAACCTGC TCGTCGCTCC
    GCACACAGCG AGCTGCCCCA CACCGAGCTC TCCAGTCAGG CTCCCTACAC CCTGAACTTC
    AGCTTTGAAG AGCTGAACAC GCTCGGCCTG GACGAAGGGG CTCCTCGCCA CAGCAACCTG
    AGTTGGCAGT CACAAACTCG CAGAACCCCA AGTCTTTCCA GTCTCACTTC CCAGGACTCC
    AGTATTGAAA TTTCAAAGCT TACTGATAAG GTGCAGGCTG AGTATCGAGA CGCTTATAGA
    GAGTACATTG CTCAGATGTC TCAGTTAGAA GGGGGCACAG GTTCCACAAC CATGAGTGGC
    AGGTCTTCTC CACACAGCAC GTATTATATG GGTCAGAGTT CATCCGGGGG CTCCATTCAC
    TCTAGTCTAG AACCCGAGAA AGGGAAGGAT AGTGAACCAA AGCAAGATGA TACACGGAAG
    TCATTTCTGA TGAAGAGGGG AGATGTTATA GATTATTCCT CATCAGGAGT TTCCACCAAT
    GATGCCTCAC CCCTGGATCC TATTACCGAG GAAGATGAAA AATCTGATCA GTCAGGCAGT
    AAGCTTCTCC CAGGCAAGAA GTCCTCAGAA AGGTCAAGTC TCTTCCAGAC AGACCTGAAG
    CTGAAGGGAG GTGGACTGCG CTATCAGAAA CTCCCGAGTG ACGAAGACGA GTCTGGGACA
    GAAGAGTCAG ACAACACTCC ACTGCTCAAA GACGACAAAG ACAAGAAAGC TGAAGGGAAA
    GCAGAGAGAG CATTGAAGTC TCCAGAGCAC AGTGCAGAGC CCATTCGAAC CTTCATCAAG
    GCCAAAGAGT ACTTGTCAGA TGCCCTCCTT GACAAAAAGG ACTCCTCTGA CTCAGGAGTG
    AGGTCCAATG AGAGCTCGCC CAACCACTCC CTTCACAACG AGGCGGCAGA TGACTCCCAG
    CTTGAAAAGG CCAACCTCAT AGAGCTCGAA GATGACCCCC ACAGTGGAAG ACGGGGAGTC
    CCGCACAGCC TGAGTGGCTT GCCAGACCCC CTCGTAGCCC GGATGTCCAT TTGCTCCGAG
    GACAAGAAGA GCCCTTCTGA ATGCAGCTTG ATAGCCAGCA GCCCCGAGGA AAACTGGCCA
    GCATGCCACA AATCCTACAA CCTGAATCGC ACCCCCAGCA CCGTGACCCT GAACAACAAC
    AGTGCCCCGG CTAACAGAGC CAACCAGAAT TCCGATGAGA CGGAGGGGCT GAGGGAGACG
    CCTCAAGTCC TCCTGAGGTC AGGCTCCAGT TCCCACTCCA CCGCTCTGCA GAATGAGAAC
    CTGAAGAGCA TGGCACACAA GCGAGGCCAG CGTTCCAGCT ACACGAGGCT CTCAAAGGAC
    TCCTCAGAGC TCCACGCAGT AGCCTCCTCC GATAGTACAG GTTTTGGAGA AGAAAGGGAG
    AGCATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53365
    Clone ID Related Accession (Same CDS sequence) XM_015465442.1 , XM_010810307.3 , XM_010810307.2
    Accession Version XM_010810307.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinase D-interacting substrate of 220 kDa isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810307.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTACCGTGT
    GACTCTGGGT TTAACAAACA GAGACAGGGA TCAGGCCCAG CCTCAGGCAC AATACCACTG
    AATTCGATGA ACGTGGATGC AGTTTGTGAG AAACTGAAAC AAATTGAAGG GCTGGACCAG
    AGCATGCTGC CTCAGTACTG TGCAACCATC AAAAAGGCGA ACATAAATGG CCGTGTGTTG
    GCTCAGTGTA ACATTGATGA ACTGAAGAAG GAAATGAATA TGAATTTTGG AGACTGGCAT
    CTTTTTAGAA GCACAATATT AGAAATGAGA AATGCAGAAA ACCAAGTGGT CCCTGAAGAC
    CCACGTTTAC TGAGTGAAAA CAGCAGCTCT GTTCCTCACG GTGAACCTGC TCGTCGCTCC
    GCACACAGCG AGCTGCCCCA CACCGAGCTC TCCAGTCAGG CTCCCTACAC CCTGAACTTC
    AGCTTTGAAG AGCTGAACAC GCTCGGCCTG GACGAAGGGG CTCCTCGCCA CAGCAACCTG
    AGTTGGCAGT CACAAACTCG CAGAACCCCA AGTCTTTCCA GTCTCACTTC CCAGGACTCC
    AGTATTGAAA TTTCAAAGCT TACTGATAAG GTGCAGGCTG AGTATCGAGA CGCTTATAGA
    GAGTACATTG CTCAGATGTC TCAGTTAGAA GGGGGCACAG GTTCCACAAC CATGAGTGGC
    AGGTCTTCTC CACACAGCAC GTATTATATG GGTCAGAGTT CATCCGGGGG CTCCATTCAC
    TCTAGTCTAG AACCCGAGAA AGGGAAGGAT AGTGAACCAA AGCAAGATGA TACACGGAAG
    TCATTTCTGA TGAAGAGGGG AGATGTTATA GATTATTCCT CATCAGGAGT TTCCACCAAT
    GATGCCTCAC CCCTGGATCC TATTACCGAG GAAGATGAAA AATCTGATCA GTCAGGCAGT
    AAGCTTCTCC CAGGCAAGAA GTCCTCAGAA AGGTCAAGTC TCTTCCAGAC AGACCTGAAG
    CTGAAGGGAG GTGGACTGCG CTATCAGAAA CTCCCGAGTG ACGAAGACGA GTCTGGGACA
    GAAGAGTCAG ACAACACTCC ACTGCTCAAA GACGACAAAG ACAAGAAAGC TGAAGGGAAA
    GCAGAGAGAG CATTGAAGTC TCCAGAGCAC AGTGCAGAGC CCATTCGAAC CTTCATCAAG
    GCCAAAGAGT ACTTGTCAGA TGCCCTCCTT GACAAAAAGG ACTCCTCTGA CTCAGGAGTG
    AGGTCCAATG AGAGCTCGCC CAACCACTCC CTTCACAACG AGGCGGCAGA TGACTCCCAG
    CTTGAAAAGG CCAACCTCAT AGAGCTCGAA GATGACCCCC ACAGTGGAAG ACGGGGAGTC
    CCGCACAGCC TGAGTGGCTT GCCAGACCCC CTCGTAGCCC GGATGTCCAT TTGCTCCGAG
    GACAAGAAGA GCCCTTCTGA ATGCAGCTTG ATAGCCAGCA GCCCCGAGGA AAACTGGCCA
    GCATGCCACA AATCCTACAA CCTGAATCGC ACCCCCAGCA CCGTGACCCT GAACAACAAC
    AGTGCCCCGG CTAACAGAGC CAACCAGAAT TCCGATGAGA CGGAGGGGCT GAGGGAGACG
    CCTCAAGTCC TCCTGAGGTC AGGCTCCAGT TCCCACTCCA CCGCTCTGCA GAATGAGAAC
    CTGAAGAGCA TGGCACACAA GCGAGGCCAG CGTTCCAGCT ACACGAGGCT CTCAAAGGAC
    TCCTCAGAGC TCCACGCAGT AGCCTCCTCC GATAGTACAG GTTTTGGAGA AGAAAGGGAG
    AGCATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808609.1
    CDS207..5498
    Translation

    Target ORF information:

    RefSeq Version XM_010810307.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinase D-interacting substrate 220kDa (KIDINS220), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810307.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCAGTTC TAATATCACA GAGTGTAATA AATTATGTAG AAGAAGAAAA CATTCCTGCT 
    TTGAAAGCTC TTCTTGAAAA ATGCAAAGAT GTTGATGAGA GAAATGAGTG TGGCCAGACT
    CCGTTGATGA TAGCTGCTGA ACAAGGCAAT CTGGAAATAG TGAAAGAATT AATTAAGAAT
    GGCGCTAATT GTAATCTGGA AGATTTGGAT AACTGGACAG CGCTTATATC AGCCTCAAAA
    GAAGGGCACC TGCACGTTGT GGACGAGCTG CTCAAGTGCG GGGCCAGCTT GGAACACCGG
    GATATGGGAG GATGGACGGC TCTTATGTGG GCGTGTTATA AAGGCCGTAC TGAAGTGGTC
    GAGCTGCTGC TTTCTCATGG TGCCAATCCA AGTGTTACTG GTCTGCAGTA CAGTGTCTAC
    CCAATCATTT GGGCAGCAGG CAGAGGCCAT GCTGATATAG TGCAACTTTT ACTGCAAAAT
    GGTGCCAAGG TCAACTGCTC TGACAAGTAT GGAACCACCC CTTTGGTTTG GGCTGCACGA
    AAAGGTCATT TGGAATGTGT AAAACATTTA TTAGCCATGG GAGCTGATGT TGATCAAGAA
    GGAGCTAATT CAATGACCGC ACTTATTGTG GCAGTAAAAG GAGGCTACAC ACAGTCAGTG
    AAAGAAATTT TGAAGCGAAA TCCAAATGTA AATTTAACAG ATAAGGATGG AAATACAGCC
    TTGATGATTG CATCGAAGGA GGGACATACA GAAATTGTAC AGGATCTCCT TGACGCTGGG
    ACATATGTGA ACATACCTGA TAGGAGTGGA GATACTGTCT TAATTGGTGC TGTCAGAGGT
    GGTCATGTGG AAATTGTTCG AGCACTTCTC CAAAAATATG CTGATATCGA CATTAGAGGA
    CAGGACAGTA AAACTGCCTT GTATTGGGCT GTTGAGAAAG GAAATGCAAC AATGGTGAGA
    GACATCTTAC AGTGCAATCC TGACACTGAA ATATGCACGA AGGATGGCGA AACACCCCTT
    ATAAAGGCTA CCAAGATGAG AAACATAGAA GTGGTAGAGC TGCTGCTGGA TAAAGGAGCC
    AAAGTGTCTG CTGTCGATAA GAAAGGAGAC ACCCCTCTGC ACATTGCTAT TCGTGGGAGG
    AGTCGGAAAC TGGCAGAACT CCTTTTAAGA AACCCCAAAG ATGGACGGTT ACTGTATAGA
    CCCAACAAAG CAGGCGAGAC TCCCTACAAT ATTGACTGTA GCCATCAGAA AAGTATTTTA
    ACTCAAATAT TTGGAGCCAG ACACTTGTCT CCTACCGAGA CAGATGGTGA CATGCTTGGT
    TACGATCTGT ACAGCAGTGC CCTGGCAGAT ATTCTCAGTG AGCCTACCAT GCAGCCGCCC
    ATTTGTGTGG GCCTGTATGC ACAGTGGGGA AGTGGTAAAT CCTTCTTACT CAAGAAACTA
    GAAGATGAAA TGAAAACTTT TGCAGGACAA CAGATTGAAC CTCTGTTTCA GTTTTCATGG
    CTTGTGGTGT TTCTGACCCT GCTCCTTTGT GGAGGGCTTG GTTTATTGTT TGCTTTTACA
    GTAGACCTAA ATCTTGGAAT TGCAGTGTCA TTGAGCTTCT TGGCTGTTTT GTATATATTT
    TTTATTGTCA TTTACTTTGG TGGACGGAGG GAAGGAGAGA GTTGGAATTG GGCCTGGGTC
    CTCAGTACTA GATTGGCAAG ACATATCGGC TATTTGGAGC TCCTCCTCAA ATTGATGTTT
    GTGAACCCAC CTGAGCTGCC AGAGCAAACC ACTAAAGCTC TACCTGTGAG GTTTTTGTTT
    ACAGATTATA ATAGACTGTC CAGCGTAGGT GGGGAAACTT CAATGGCTGA AATGATCGCC
    ACCCTCTCGG ATGCTTGTGA AAGAGAGTTT GGCTTTTTGG CAACCAGGCT TTTCCGAGTG
    TTCAAGACTG AAGATACGCA GGGTAAAAAG AAATGGAAAA AAACGTGTTG TCTCCCATCT
    TTTGTCATCT TCCTTTTCAT CTTTGGCTGC ATTATTGCTG CGATTACTCT TCTGGCTATA
    TTCAGAGTTG ACCCGAAGCA TATGACAGTG AATGCTGTCC TCATATCAAT TGCGTCCATA
    GTGGGGTTGG CGTTTGTGCT GAACTGTCGA ACATGGTGGC AGGTGCTGGA CTCACTTCTG
    AATTCTCAAA GGAAACGCCT CCATAATGCT GCCTCCAAAC TGCACAAACT GAAAAGTGAA
    GGATTCATGA AAGTTCTTAA GTGTGAAGTG GAATTGATGG CCAGGATGGC AAAAACCATT
    GACAGCTTCA CTCAGAATCA GACAAGGCTG GTGGTTATCA TCGATGGGTT AGATGCCTGT
    GAGCAGGACA AAGTACTCCA GATGCTGGAC ACTGTCCGAG TTCTGTTTTC AAAAGGCCCA
    TTCATTGCCA TTTTTGCAAG TGATCCACAT ATTATCATAA AGGCAATTAA TCAGAACCTC
    AATAGTGTGC TTCGTGACTC AAACATCAAT GGACATGACT ATATGCGCAA TATAGTTCAT
    CTGCCCGTTT TCCTCAACAG CCGGGGACTA AGCAGTGCAA GAAAATTTCT TGTAACTTCA
    ACAACTAATG GAGATGTTCC ATGCTCGGAT AATACAGGGA TGCAGGAAGA TGCTGACAGA
    AGAGTTTCAC AAAACAGCCT TGGAGAGATG ACAAAACTTG GTAGCAAGAC GGCCCTCAAT
    AGACGGGACA CTTACCGAAG AAGGCAGATG CAGCGCACGA TTACCCGGCA GATGTCCTTC
    GACCTCACGA AACTGCTGGT TACGGAGGAC TGGTTCAGTG ACATAAGCCC GCAGACCATG
    AGGAGACTGC TCAACATTGT TTCTGTGACA GGACGATTAC TGAGAGCCAA CCAGATAAGT
    TTCAACTGGG ATAGGCTTGC TAGCTGGATC AACCTAACTG AGCAGTGGCC GTACAGAACT
    TCATGGCTCA TATTGTATTT GGAAGAGACC GAAGGTATCC CAGATCAAAT GACATTAAAA
    ACCATCTATG AAAGAATATC AAAGAATATT CCAACAACTA AAGATGTTGA GCCACTGCTT
    GAAATAGATG GAGATATAAG AAATTTTGAA GTGTTTTTGT CTTCAAGGAC CCCAGTTCTT
    GTGGCTCGAG ATGTGAAAAC CTTTTTGCCA TGCACTGTAA ATCTGGACCC CAAACTGCGG
    GAGATTATTG CAGATGTTCG TGCTGCAAGA GACCAGATTA ATATCGGAGG ACTTGCGTAT
    CCCCCGCTAC CTCTGCACGA GCCTCCCCCT CGACCGCCGT CCGGCTACAG TCAGCCAGCG
    TCTGTCTGCT CTTCGTCCAC CTCCTTCAAT GGGCCATTCG GGGGAGGGGT TGTGTCGCCG
    CAGCCTCATA GCAGCTACTA CAGCGGCATG ACAGGACCCC AGCACCCCTT CTACAACAGG
    CCATTCTTTG CCCCCTACCT TTACACGCCA AGGTACTGCC CTGGCGGCTC CCACCATCTC
    ATCTCACGTC CATCAGTAAA AACGAATTTG CCCAGAGATC AGAGCAATGG CCTACCGTGT
    GACTCTGGGT TTAACAAACA GAGACAGGGA TCAGGCCCAG CCTCAGGCAC AATACCACTG
    AATTCGATGA ACGTGGATGC AGTTTGTGAG AAACTGAAAC AAATTGAAGG GCTGGACCAG
    AGCATGCTGC CTCAGTACTG TGCAACCATC AAAAAGGCGA ACATAAATGG CCGTGTGTTG
    GCTCAGTGTA ACATTGATGA ACTGAAGAAG GAAATGAATA TGAATTTTGG AGACTGGCAT
    CTTTTTAGAA GCACAATATT AGAAATGAGA AATGCAGAAA ACCAAGTGGT CCCTGAAGAC
    CCACGTTTAC TGAGTGAAAA CAGCAGCTCT GTTCCTCACG GTGAACCTGC TCGTCGCTCC
    GCACACAGCG AGCTGCCCCA CACCGAGCTC TCCAGTCAGG CTCCCTACAC CCTGAACTTC
    AGCTTTGAAG AGCTGAACAC GCTCGGCCTG GACGAAGGGG CTCCTCGCCA CAGCAACCTG
    AGTTGGCAGT CACAAACTCG CAGAACCCCA AGTCTTTCCA GTCTCACTTC CCAGGACTCC
    AGTATTGAAA TTTCAAAGCT TACTGATAAG GTGCAGGCTG AGTATCGAGA CGCTTATAGA
    GAGTACATTG CTCAGATGTC TCAGTTAGAA GGGGGCACAG GTTCCACAAC CATGAGTGGC
    AGGTCTTCTC CACACAGCAC GTATTATATG GGTCAGAGTT CATCCGGGGG CTCCATTCAC
    TCTAGTCTAG AACCCGAGAA AGGGAAGGAT AGTGAACCAA AGCAAGATGA TACACGGAAG
    TCATTTCTGA TGAAGAGGGG AGATGTTATA GATTATTCCT CATCAGGAGT TTCCACCAAT
    GATGCCTCAC CCCTGGATCC TATTACCGAG GAAGATGAAA AATCTGATCA GTCAGGCAGT
    AAGCTTCTCC CAGGCAAGAA GTCCTCAGAA AGGTCAAGTC TCTTCCAGAC AGACCTGAAG
    CTGAAGGGAG GTGGACTGCG CTATCAGAAA CTCCCGAGTG ACGAAGACGA GTCTGGGACA
    GAAGAGTCAG ACAACACTCC ACTGCTCAAA GACGACAAAG ACAAGAAAGC TGAAGGGAAA
    GCAGAGAGAG CATTGAAGTC TCCAGAGCAC AGTGCAGAGC CCATTCGAAC CTTCATCAAG
    GCCAAAGAGT ACTTGTCAGA TGCCCTCCTT GACAAAAAGG ACTCCTCTGA CTCAGGAGTG
    AGGTCCAATG AGAGCTCGCC CAACCACTCC CTTCACAACG AGGCGGCAGA TGACTCCCAG
    CTTGAAAAGG CCAACCTCAT AGAGCTCGAA GATGACCCCC ACAGTGGAAG ACGGGGAGTC
    CCGCACAGCC TGAGTGGCTT GCCAGACCCC CTCGTAGCCC GGATGTCCAT TTGCTCCGAG
    GACAAGAAGA GCCCTTCTGA ATGCAGCTTG ATAGCCAGCA GCCCCGAGGA AAACTGGCCA
    GCATGCCACA AATCCTACAA CCTGAATCGC ACCCCCAGCA CCGTGACCCT GAACAACAAC
    AGTGCCCCGG CTAACAGAGC CAACCAGAAT TCCGATGAGA CGGAGGGGCT GAGGGAGACG
    CCTCAAGTCC TCCTGAGGTC AGGCTCCAGT TCCCACTCCA CCGCTCTGCA GAATGAGAAC
    CTGAAGAGCA TGGCACACAA GCGAGGCCAG CGTTCCAGCT ACACGAGGCT CTCAAAGGAC
    TCCTCAGAGC TCCACGCAGT AGCCTCCTCC GATAGTACAG GTTTTGGAGA AGAAAGGGAG
    AGCATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.