ADAMTS20 cDNA ORF clone, Bos taurus(cattle)

The following ADAMTS20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADAMTS20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa239046 NM_001206093.2
Latest version!
Bos taurus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa234494 XM_024991940.1
Latest version!
Bos taurus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa234495 XM_024991941.1
Latest version!
Bos taurus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa19305 NM_001206093.1
Latest version!
Bos taurus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa53323 XM_015471029.1
Latest version!
Bos taurus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa53325 XM_010805067.2
Latest version!
Bos taurus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa239046
    Clone ID Related Accession (Same CDS sequence) NM_001206093.2
    Accession Version NM_001206093.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000005.1. On May 30, 2018 this sequence version replaced NM_001206093.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN04019636, SAMN04019639 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCGGGTGG TCAAGTGGCT GACGGGGCTG CTCTACCAGC TCTCGCTCTT CATCACCAGG 
    TCTTGGGAAG TTCACTTCCA CCCCAGGCAG GAAGCCTTAG TGAGGACACT GGCCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAATGAG TTCGGCGAAG TATTCCCACA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCGGAGACT CTGGAGCCCA CACCGTTCAG AACCCACTAC
    CGAATCCGCG CCTACGGGCA GCTCTTCCAG CTGAACCTGA GCGCCGACGC GGCCTTCCTG
    GCTGCGGGAT ACACCGAGGT GCACTTAGGC ACTTCTGCGC ACCCGGCGGA GGAGCGCAGC
    GCGGCGCCCC CAGACCTACG CCACTGCTTC TACCGCGGCC AGGTCAATGC TCGCGAGGAT
    CACACAGCCG TCTTCAGCCT CTGTGGAGGC TTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGTG AACATGAAGA TGATCATAAC
    AAGCCACATC TTATATACAG ACAGGAATTA AAGAGGAACT ATTTTCTGCA GTCTCACAAG
    CCTTGTGATG TTTCAGAAAG TGAAATAAAG AAAACTACTT TACCCTTTCA TAACTACAGC
    GACGTGAATG AAAATCTCAA TATAAAGGAG GAAAGAGTTT TAGGATACAC TTCAAAAAAT
    ATATCTTTGG AAGATGAAAG AAGTCAATTG CATTCCAGGA AAAAACGTTT TTTATCATAT
    CCAAGATACG TGGAAGTTAT GGTTACAGCT GACTCTAAAA TGGTTCATCA TCACGGACAG
    AACTTGCAGC ACTATGTACT AACTCTGATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTTATAAA CATAGTAATT GTAAAATTAG TTATAATTCA TGATGAGCAG
    GAAGGACCAG TCATCAGTTT TAATGCAGCT ACCACATTAC GCAACTTCTG TTTATGGCAA
    CAAACTCAAA ATGTTCTTGA CGATGCCCAC CCATCCCACC ATGATACTGC TGTTCTTATC
    ACAAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTAGGTTT AGCAGAATTA
    GGTACCCTGT GCGATCCTTT ACGGAGCTGC TCCATTAGTG AAGAAAATGG ACTCAGTGCT
    GCCTTTACCA TAGCCCATGA GCTTGGTCAT GTATTTAATG TTCCACACGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAT TAAACATCAG TATCATGTCA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACCGGTCATG GGGAATGCCT CCTTGACAAA CCAAATGGAA GAATATATGA TCTATCTTCA
    CAGCTTCCTG GATTAATGTA TGATGTAAAC AAGCAATGTG AGCTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCCTATTT AAAACAATGC AGGCGTCTCT GGTGTACCAG TGCAGAAGGA
    GTTCACAAGG GCTGTCGCAC TCAACACATG CCACTGGCAG ATGGAACAAA CTGTGGTCCT
    GGGATGCATT GCCACCATGG GCTATGTGTA AATAAAGAAA TGGAAACACG TCCTGTAGAT
    GGCGATTGGG GACCATGGGG ACCTTACAGT TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA GCAGGCTCTG TAATCGCCCC GAGCCAAGAA ACGGAGGCAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAC ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTCCGTGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTCTCC
    CCTAACGTGA GGTGGCTTCC AAAGTACAGT GGGATTGCTG TAAAAGATCG TTGTAAACTC
    TATTGTCGGG TGGCTGGAAC CACTAGCTTC TACCAACTGA AGGACAGGGT TGCTGATGGC
    ACTCCTTGTG GAACTGAAAC AAATGACATC TGTGTTCAAG GCCTATGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAGT GTGGTGTGTG TGGTGGGGAT
    AACTCCTCCT GCAGAACAAT GGCAGGTGTC TTCAACAGTG CACATTATGG TTATAATGTC
    GTTGTAAAGA TTCCCCCAGG AGCAACAAAC ATTGATATTC TTCAGCACAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTGGCATTA TCGGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCCAAAAGG GAAATCAACG TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAACTC AATTGAAAGG ATTAATAGTA CTGATCGACT GGAAGAAGAA
    CTTGTTTTGC AGGTTTTGTG TGTGGGTAAT CTATACAACC CTGATGTACG TTATTCCTTC
    AATATCCCCG TGGAAGAGAA GAGTGATCTA TTCACATGGG ACCCATATGG ACCGTGGCAA
    GACTGTACAA AAATGTGTCA AGGTCTTCAT AGAAGAAAAA TTACTTGTGT ACGTAAGAGT
    GATCATATGG TCGTGTCTGA TCAAAGCTGT GACCACATAC CACTTCCATT GCTGGTGACT
    GAAAGGTGCA ATACAGACTG TGAACTAAGG TGGCACATCA TTGGCAAAAG TGAATGTTCA
    GCCCTTTGCG GTCAAGGATA CAAGTCACTG GACGTCCATT GTATGAAGTA TTCCATTCAT
    AACGGACAGA CTGTTCCAGT AGTTGACCAC TACTGCAGTG ACCAACTTAA GCCTCCAACC
    CGAGAACCCT GCCATGGTGA TTGTGTCTTA ACAAGGTGGC ATTATTTAGA ATGGTCTCAG
    TGCTCCAGAA GTTGTGGAGG AGGGGAGAGG TCTCGAGACT CTTACTGTAT AAATAACTTT
    GGCCACCGTC TTGCTGACAG AGAATGCCAG GAACTTCCCC GAGTGACAGT AGAGAATTGC
    AATGAATTTT CCTGTCCCAG TTGGGCCACT AGTGAGTGGA GTGAGTGTCT GGTTACCTGT
    GGAAAAGGAA CAAACCAGCG GCAGGTGTGG TGTCAACTGA ATGAAGATCA CTTGAGTGAC
    GGCTTCTGTG ACCCAAGCAC CAAGCCTGAG TCTCAGAGAC CCTGTGAGCT TCATGCGTGT
    ACTTCCTGGC AAGTAGGACC TTGGGGTTCT TGCACAGCCA CGTGTGGACA GGGGTACCAG
    ATGCGTGCTG TGAAATGTGT CAACGAGCTA TTTAGTGCAG TGCTAGATGA CAGACTGTGC
    CATGGAGCTA GTCGCCCAAG TGAAAGGCAG GATTGTATTG TTATACCTTG TCCAGTTATC
    CCTAAAATTG GGGCCACTTC CTTGCCAGCT GTTCCCATGA GAAAGATAAC ACAATGGCGA
    TATGGTTCTT GGACCCCATG CTCTGTGTCT TGTGGAAGAG GAAATCAAGC CCGCTATGTA
    AGTTGTCGTG ATGCTCATGA TGGAATAGCA GATGAATCAT TTTGTGCCCA CTTACCCCGA
    CCAGCTGAAA TAGCTGTCTG TTTTAGCCTT TGTGGAGAGT GGCAAACAGG GAATTGGTCA
    CCTTGTTCAG CTTCCTGTGG CCGTGGAAAA ATGACTCGCC AAGTTTTATG CATCAACTAT
    CATCAGCCAG TTAATGAGAA TTACTGTGAC CCCGAAGTCC GCCCTGTGAT AGAACAAGAA
    TGTAATCTGG CAGCCTGCCC TCCCACGTAC AGCCACTTTC CGAGTTCCTC TGAGCAGCCA
    AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAAGATAA TCAAAATCAG
    GGGATCCATC CATCAATCAG AGAAAACCAG TGGAGAACAG GACCATGGGG ATCATGCTCT
    AGCAGTTGTG CTGGGGGTGT TCAACATCGA GTTGTGGTCT GCCAGGATGA AAATGGACAA
    AGTGCCAGTT ACTGTGATTC AGCGTCCAAA CCCCCAGAGT CAAAGCACTG TGATTCGGGA
    CCTTGTCCAC AGTGGAACTA TGGAAGCTGG GGAGAATGTA CACAAACATG TGGAGGAGGA
    ATAAAATCGA GATTTGTAAT ATGTCAGTTT CCCAATGGCC AACTGTCACA AGAGCAAAAC
    TGTGAAATGT TGAACAAGCC ACCTAGTGTG GTGCAGTGTC ATATACGTGC TTGCCCTGAT
    GATGTATCAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCCTGTGG TAAAGGTTTA
    AAATACCGTG AGGTGCTTTG CATTGACCAA TTCCACGGGA AATTAGAAGA AAAATATTGC
    AGTCATCTAC AGAAACCTCG AACGCATAAA GCTTGTAGGT CTGTCCAATG CCCTTCATGG
    AAAGCCAACA GATGGAAGGA GTGCTCTGTG ACCTGCGGCT CCGGAGTTCA GCAACGGGAT
    GTGTACTGCA GACTGAGAGG AGTTGGTCGG GTGGCGGAGG AAAAGTGTGA TCCATCCACA
    CGGCCTTATT TTCAGAGAGA GTGTTGGCAT CGGAACTGCA CACAGTACCA CTGGGTGGCA
    GGAGAATGGT TGGATTGCTC AATATCGTGT AAGAAAAGAG AGACATATCG GCAAGTGAAG
    TGCGTGGATG CACAAAACAT TGAAGTGAGT GAGAGTTTCT GTGACCTCGC AACCAGACCT
    CTCGCGATCA AAAAGTGCAG AAACTCTCCC TGCAAGTATA TCGTGATAAC AGGAGACTCA
    TCCCAGTGTG CAGGTAACTG TGGATTTAGT TATCGACAAA GGATTACATA TTGCATTGGG
    GTCCAATCTA TGGAGAAACA TAAGCTTCAT GAGCTGTGGC CTATAGGCTA TCGAGTATGC
    CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA AGACCTGTTT GCATATGGCC
    ACTTGGAAAG TTGGAAAATG GAGCAAGTGC TCTGTGACTT GTGGAGTTGG GATGATGGAG
    AGAAGAGTGG AATGCATGGC CGATAATGGT CTGTCCAGTG ATTTATGTTT AAAGCATTTA
    AAACCAGATG CTCAAAAGAA ATGTTATGTC AGTGACTGTA AAACATTTAC CAGCTGTAAA
    GAAATACAAA TGAAAAACAA CATTACCAAG GATGGTGACT ATTACCTTAA CATTAAAGGA
    AGAATAACAA AGATCTATTG TGCTGGCATG CACTTGCAGA ACCCTAAGGA ATATATATCA
    TTGGTCAAAG GTGAAGAAGA CAACTTTTCT GAAGTGTATG GTTTTAGACT ACGAAATCCA
    TATGAATGTC CCTTTAATGG AAGCAGGAGG CAAGACTGTG AATGTAAAAA TGACTATATG
    GCTGCTGGAC ACACTGTTTT TAGCAAAATA AGAATTGATC TTACTTCCAT GCAAATTAAA
    ACTACAGACC TTCTTTTTGC CCAGACAATA TTTGGAAATG CAGTTCCATT TGCCACAGCT
    GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGACAGT TCAGCATTAA TTTGGCAGGA
    ACTGGGATGA AGATATCCAG TACAGCAAAG TGGCTGGCTC AGGGCAGTTA TGCCTCTGTG
    ATCATTCACA GATCACAGGA TGGAACCAAA GTCTATGGCA GATGTGGAGG ATTCTGTGGA
    AAGTGTATTC CTCACATGAC TACTGGTCTC CCAATTCAAG TAGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193022.1
    CDS1..5748
    Translation

    Target ORF information:

    RefSeq Version NM_001206093.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206093.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCGGGTGG TCAAGTGGCT GACGGGGCTG CTCTACCAGC TCTCGCTCTT CATCACCAGG 
    TCTTGGGAAG TTCACTTCCA CCCCAGGCAG GAAGCCTTAG TGAGGACACT GGCCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAATGAG TTCGGCGAAG TATTCCCACA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCGGAGACT CTGGAGCCCA CACCGTTCAG AACCCACTAC
    CGAATCCGCG CCTACGGGCA GCTCTTCCAG CTGAACCTGA GCGCCGACGC GGCCTTCCTG
    GCTGCGGGAT ACACCGAGGT GCACTTAGGC ACTTCTGCGC ACCCGGCGGA GGAGCGCAGC
    GCGGCGCCCC CAGACCTACG CCACTGCTTC TACCGCGGCC AGGTCAATGC TCGCGAGGAT
    CACACAGCCG TCTTCAGCCT CTGTGGAGGC TTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGTG AACATGAAGA TGATCATAAC
    AAGCCACATC TTATATACAG ACAGGAATTA AAGAGGAACT ATTTTCTGCA GTCTCACAAG
    CCTTGTGATG TTTCAGAAAG TGAAATAAAG AAAACTACTT TACCCTTTCA TAACTACAGC
    GACGTGAATG AAAATCTCAA TATAAAGGAG GAAAGAGTTT TAGGATACAC TTCAAAAAAT
    ATATCTTTGG AAGATGAAAG AAGTCAATTG CATTCCAGGA AAAAACGTTT TTTATCATAT
    CCAAGATACG TGGAAGTTAT GGTTACAGCT GACTCTAAAA TGGTTCATCA TCACGGACAG
    AACTTGCAGC ACTATGTACT AACTCTGATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTTATAAA CATAGTAATT GTAAAATTAG TTATAATTCA TGATGAGCAG
    GAAGGACCAG TCATCAGTTT TAATGCAGCT ACCACATTAC GCAACTTCTG TTTATGGCAA
    CAAACTCAAA ATGTTCTTGA CGATGCCCAC CCATCCCACC ATGATACTGC TGTTCTTATC
    ACAAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTAGGTTT AGCAGAATTA
    GGTACCCTGT GCGATCCTTT ACGGAGCTGC TCCATTAGTG AAGAAAATGG ACTCAGTGCT
    GCCTTTACCA TAGCCCATGA GCTTGGTCAT GTATTTAATG TTCCACACGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAT TAAACATCAG TATCATGTCA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACCGGTCATG GGGAATGCCT CCTTGACAAA CCAAATGGAA GAATATATGA TCTATCTTCA
    CAGCTTCCTG GATTAATGTA TGATGTAAAC AAGCAATGTG AGCTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCCTATTT AAAACAATGC AGGCGTCTCT GGTGTACCAG TGCAGAAGGA
    GTTCACAAGG GCTGTCGCAC TCAACACATG CCACTGGCAG ATGGAACAAA CTGTGGTCCT
    GGGATGCATT GCCACCATGG GCTATGTGTA AATAAAGAAA TGGAAACACG TCCTGTAGAT
    GGCGATTGGG GACCATGGGG ACCTTACAGT TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA GCAGGCTCTG TAATCGCCCC GAGCCAAGAA ACGGAGGCAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAC ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTCCGTGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTCTCC
    CCTAACGTGA GGTGGCTTCC AAAGTACAGT GGGATTGCTG TAAAAGATCG TTGTAAACTC
    TATTGTCGGG TGGCTGGAAC CACTAGCTTC TACCAACTGA AGGACAGGGT TGCTGATGGC
    ACTCCTTGTG GAACTGAAAC AAATGACATC TGTGTTCAAG GCCTATGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAGT GTGGTGTGTG TGGTGGGGAT
    AACTCCTCCT GCAGAACAAT GGCAGGTGTC TTCAACAGTG CACATTATGG TTATAATGTC
    GTTGTAAAGA TTCCCCCAGG AGCAACAAAC ATTGATATTC TTCAGCACAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTGGCATTA TCGGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCCAAAAGG GAAATCAACG TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAACTC AATTGAAAGG ATTAATAGTA CTGATCGACT GGAAGAAGAA
    CTTGTTTTGC AGGTTTTGTG TGTGGGTAAT CTATACAACC CTGATGTACG TTATTCCTTC
    AATATCCCCG TGGAAGAGAA GAGTGATCTA TTCACATGGG ACCCATATGG ACCGTGGCAA
    GACTGTACAA AAATGTGTCA AGGTCTTCAT AGAAGAAAAA TTACTTGTGT ACGTAAGAGT
    GATCATATGG TCGTGTCTGA TCAAAGCTGT GACCACATAC CACTTCCATT GCTGGTGACT
    GAAAGGTGCA ATACAGACTG TGAACTAAGG TGGCACATCA TTGGCAAAAG TGAATGTTCA
    GCCCTTTGCG GTCAAGGATA CAAGTCACTG GACGTCCATT GTATGAAGTA TTCCATTCAT
    AACGGACAGA CTGTTCCAGT AGTTGACCAC TACTGCAGTG ACCAACTTAA GCCTCCAACC
    CGAGAACCCT GCCATGGTGA TTGTGTCTTA ACAAGGTGGC ATTATTTAGA ATGGTCTCAG
    TGCTCCAGAA GTTGTGGAGG AGGGGAGAGG TCTCGAGACT CTTACTGTAT AAATAACTTT
    GGCCACCGTC TTGCTGACAG AGAATGCCAG GAACTTCCCC GAGTGACAGT AGAGAATTGC
    AATGAATTTT CCTGTCCCAG TTGGGCCACT AGTGAGTGGA GTGAGTGTCT GGTTACCTGT
    GGAAAAGGAA CAAACCAGCG GCAGGTGTGG TGTCAACTGA ATGAAGATCA CTTGAGTGAC
    GGCTTCTGTG ACCCAAGCAC CAAGCCTGAG TCTCAGAGAC CCTGTGAGCT TCATGCGTGT
    ACTTCCTGGC AAGTAGGACC TTGGGGTTCT TGCACAGCCA CGTGTGGACA GGGGTACCAG
    ATGCGTGCTG TGAAATGTGT CAACGAGCTA TTTAGTGCAG TGCTAGATGA CAGACTGTGC
    CATGGAGCTA GTCGCCCAAG TGAAAGGCAG GATTGTATTG TTATACCTTG TCCAGTTATC
    CCTAAAATTG GGGCCACTTC CTTGCCAGCT GTTCCCATGA GAAAGATAAC ACAATGGCGA
    TATGGTTCTT GGACCCCATG CTCTGTGTCT TGTGGAAGAG GAAATCAAGC CCGCTATGTA
    AGTTGTCGTG ATGCTCATGA TGGAATAGCA GATGAATCAT TTTGTGCCCA CTTACCCCGA
    CCAGCTGAAA TAGCTGTCTG TTTTAGCCTT TGTGGAGAGT GGCAAACAGG GAATTGGTCA
    CCTTGTTCAG CTTCCTGTGG CCGTGGAAAA ATGACTCGCC AAGTTTTATG CATCAACTAT
    CATCAGCCAG TTAATGAGAA TTACTGTGAC CCCGAAGTCC GCCCTGTGAT AGAACAAGAA
    TGTAATCTGG CAGCCTGCCC TCCCACGTAC AGCCACTTTC CGAGTTCCTC TGAGCAGCCA
    AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAAGATAA TCAAAATCAG
    GGGATCCATC CATCAATCAG AGAAAACCAG TGGAGAACAG GACCATGGGG ATCATGCTCT
    AGCAGTTGTG CTGGGGGTGT TCAACATCGA GTTGTGGTCT GCCAGGATGA AAATGGACAA
    AGTGCCAGTT ACTGTGATTC AGCGTCCAAA CCCCCAGAGT CAAAGCACTG TGATTCGGGA
    CCTTGTCCAC AGTGGAACTA TGGAAGCTGG GGAGAATGTA CACAAACATG TGGAGGAGGA
    ATAAAATCGA GATTTGTAAT ATGTCAGTTT CCCAATGGCC AACTGTCACA AGAGCAAAAC
    TGTGAAATGT TGAACAAGCC ACCTAGTGTG GTGCAGTGTC ATATACGTGC TTGCCCTGAT
    GATGTATCAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCCTGTGG TAAAGGTTTA
    AAATACCGTG AGGTGCTTTG CATTGACCAA TTCCACGGGA AATTAGAAGA AAAATATTGC
    AGTCATCTAC AGAAACCTCG AACGCATAAA GCTTGTAGGT CTGTCCAATG CCCTTCATGG
    AAAGCCAACA GATGGAAGGA GTGCTCTGTG ACCTGCGGCT CCGGAGTTCA GCAACGGGAT
    GTGTACTGCA GACTGAGAGG AGTTGGTCGG GTGGCGGAGG AAAAGTGTGA TCCATCCACA
    CGGCCTTATT TTCAGAGAGA GTGTTGGCAT CGGAACTGCA CACAGTACCA CTGGGTGGCA
    GGAGAATGGT TGGATTGCTC AATATCGTGT AAGAAAAGAG AGACATATCG GCAAGTGAAG
    TGCGTGGATG CACAAAACAT TGAAGTGAGT GAGAGTTTCT GTGACCTCGC AACCAGACCT
    CTCGCGATCA AAAAGTGCAG AAACTCTCCC TGCAAGTATA TCGTGATAAC AGGAGACTCA
    TCCCAGTGTG CAGGTAACTG TGGATTTAGT TATCGACAAA GGATTACATA TTGCATTGGG
    GTCCAATCTA TGGAGAAACA TAAGCTTCAT GAGCTGTGGC CTATAGGCTA TCGAGTATGC
    CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA AGACCTGTTT GCATATGGCC
    ACTTGGAAAG TTGGAAAATG GAGCAAGTGC TCTGTGACTT GTGGAGTTGG GATGATGGAG
    AGAAGAGTGG AATGCATGGC CGATAATGGT CTGTCCAGTG ATTTATGTTT AAAGCATTTA
    AAACCAGATG CTCAAAAGAA ATGTTATGTC AGTGACTGTA AAACATTTAC CAGCTGTAAA
    GAAATACAAA TGAAAAACAA CATTACCAAG GATGGTGACT ATTACCTTAA CATTAAAGGA
    AGAATAACAA AGATCTATTG TGCTGGCATG CACTTGCAGA ACCCTAAGGA ATATATATCA
    TTGGTCAAAG GTGAAGAAGA CAACTTTTCT GAAGTGTATG GTTTTAGACT ACGAAATCCA
    TATGAATGTC CCTTTAATGG AAGCAGGAGG CAAGACTGTG AATGTAAAAA TGACTATATG
    GCTGCTGGAC ACACTGTTTT TAGCAAAATA AGAATTGATC TTACTTCCAT GCAAATTAAA
    ACTACAGACC TTCTTTTTGC CCAGACAATA TTTGGAAATG CAGTTCCATT TGCCACAGCT
    GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGACAGT TCAGCATTAA TTTGGCAGGA
    ACTGGGATGA AGATATCCAG TACAGCAAAG TGGCTGGCTC AGGGCAGTTA TGCCTCTGTG
    ATCATTCACA GATCACAGGA TGGAACCAAA GTCTATGGCA GATGTGGAGG ATTCTGTGGA
    AAGTGTATTC CTCACATGAC TACTGGTCTC CCAATTCAAG TAGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234494
    Clone ID Related Accession (Same CDS sequence) XM_024991940.1
    Accession Version XM_024991940.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGGTGG TCAAGTGGCT GACGGGGCTG CTCTACCAGC TCTCGCTCTT CATCACCAGG 
    TCTTGGGAAG TTCACTTCCA CCCCAGGCAG GAAGCCTTAG TGAGGACACT GGCCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAATGAG TTCGGCGAAG TATTCCCACA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCGGAGACT CTGGAGCCCA CACCGTTCAG AACCCACTAC
    CGAATCCGCG CCTACGGGCA GCTCTTCCAG CTGAACCTGA GCGCCGACGC GGCCTTCCTG
    GCTGCGGGAT ACACCGAGGT GCACTTAGGC ACTTCTGCGC ACCCGGCGGA GGAGCGCAGC
    GCGGCGCCCC CAGACCTACG CCACTGCTTC TACCGCGGCC AGGTCAATGC TCGCGAGGAT
    CACACAGCCG TCTTCAGCCT CTGTGGAGGC TTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGTG AACATGAAGA TGATCATAAC
    AAGCCACATC TTATATACAG ACAGGAATTA AAGAGGAACT ATTTTCTGCA GTCTCACAAG
    CCTTGTGATG TTTCAGAAAG TGAAATAAAG AAAACTACTT TACCCTTTCA TAACTACAGC
    GACGTGAATG AAAATCTCAA TATAAAGGAG GAAAGAGTTT TAGGATACAC TTCAAAAAAT
    ATATCTTTGG AAGATGAAAG AAGTCAATTG CATTCCAGGA AAAAACGTTT TTTATCATAT
    CCAAGATACG TGGAAGTTAT GGTTACAGCT GACTCTAAAA TGGTTCATCA TCACGGACAG
    AACTTGCAGC ACTATGTACT AACTCTGATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTTATAAA CATAGTAATT GTAAAATTAG TTATAATTCA TGATGAGCAG
    GAAGGACCAG TCATCAGTTT TAATGCAGCT ACCACATTAC GCAACTTCTG TTTATGGCAA
    CAAACTCAAA ATGTTCTTGA CGATGCCCAC CCATCCCACC ATGATACTGC TGTTCTTATC
    ACAAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTAGGTTT AGCAGAATTA
    GGTACCCTGT GCGATCCTTT ACGGAGCTGC TCCATTAGTG AAGAAAATGG ACTCAGTGCT
    GCCTTTACCA TAGCCCATGA GCTTGGTCAT GTATTTAATG TTCCACACGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAT TAAACATCAG TATCATGTCA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACCGGTCATG GGGAATGCCT CCTTGACAAA CCAAATGGAA GAATATATGA TCTATCTTCA
    CAGCTTCCTG GATTAATGTA TGATGTAAAC AAGCAATGTG AGCTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCCTATTT AAAACAATGC AGGCGTCTCT GGTGTACCAG TGCAGAAGGA
    GTTCACAAGG GCTGTCGCAC TCAACACATG CCACTGGCAG ATGGAACAAA CTGTGGTCCT
    GGGATGCATT GCCACCATGG GCTATGTGTA AATAAAGAAA TGGAAACACG TCCTGTAGAT
    GGCGATTGGG GACCATGGGG ACCTTACAGT TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA GCAGGCTCTG TAATCGCCCC GAGCCAAGAA ACGGAGGCAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAC ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTCCGTGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTCTCC
    CCTAACGTGA GGTGGCTTCC AAAGTACAGT GGGATTGCTG TAAAAGATCG TTGTAAACTC
    TATTGTCGGG TGGCTGGAAC CACTAGCTTC TACCAACTGA AGGACAGGGT TGCTGATGGC
    ACTCCTTGTG GAACTGAAAC AAATGACATC TGTGTTCAAG GCCTATGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAGT GTGGTGTGTG TGGTGGGGAT
    AACTCCTCCT GCAGAACAAT GGCAGGTGTC TTCAACAGTG CACATTATGG TTATAATGTC
    GTTGTAAAGA TTCCCCCAGG AGCAACAAAC ATTGATATTC TTCAGCACAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTGGCATTA TCGGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCCAAAAGG GAAATCAACG TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAACTC AATTGAAAGG ATTAATAGTA CTGATCGACT GGAAGAAGAA
    CTTGTTTTGC AGGTTTTGTG TGTGGGTAAT CTATACAACC CTGATGTACG TTATTCCTTC
    AATATCCCCG TGGAAGAGAA GAGTGATCTA TTCACATGGG ACCCATATGG ACCGTGGCAA
    GACTGTACAA AAATGTGTCA AGGTCTTCAT AGAAGAAAAA TTACTTGTGT ACGTAAGAGT
    GATCATATGG TCGTGTCTGA TCAAAGCTGT GACCACATAC CACTTCCATT GCTGGTGACT
    GAAAGGTGCA ATACAGACTG TGAACTAAGG TGGCACATCA TTGGCAAAAG TGAATGTTCA
    GCCCTTTGCG GTCAAGGATA CAAGTCACTG GACGTCCATT GTATGAAGTA TTCCATTCAT
    AACGGACAGA CTGTTCCAGT AGTTGACCAC TACTGCAGTG ACCAACTTAA GCCTCCAACC
    CGAGAACCCT GCCATGGTGA TTGTGTCTTA ACAAGGTGGC ATTATTTAGA ATGGTCTCAG
    TGCTCCAGAA GTTGTGGAGG AGGGGAGAGG TCTCGAGACT CTTACTGTAT AAATAACTTT
    GGCCACCGTC TTGCTGACAG AGAATGCCAG GAACTTCCCC GAGTGACAGT AGAGAATTGC
    AATGAATTTT CCTGTCCCAG TTGGGCCACT AGTGAGTGGA GTGAGTGTCT GGTTACCTGT
    GGAAAAGGAA CAAACCAGCG GCAGGTGTGG TGTCAACTGA ATGAAGATCA CTTGAGTGAC
    GGCTTCTGTG ACCCAAGCAC CAAGCCTGAG TCTCAGAGAC CCTGTGAGCT TCATGCGTGT
    ACTTCCTGGC AAGTAGGACC TTGGGGTTCT TGCTCTGTGT CTTGTGGAAG AGGAAATCAA
    GCCCGCTATG TAAGTTGTCG TGATGCTCAT GATGGAATAG CAGATGAATC ATTTTGTGCC
    CACTTACCCC GACCAGCTGA AATAGCTGTC TGTTTTAGCC TTTGTGGAGA GTGGCAAACA
    GGGAATTGGT CACCTTGTTC AGCTTCCTGT GGCCGTGGAA AAATGACTCG CCAAGTTTTA
    TGCATCAACT ATCATCAGCC AGTTAATGAG AATTACTGTG ACCCCGAAGT CCGCCCTGTG
    ATAGAACAAG AATGTAATCT GGCAGCCTGC CCTCCCACGT ACAGCCACTT TCCGAGTTCC
    TCTGAGCAGC CAAGCCATTT TCCAGGCAGG AATTTTCCAT TAACTCACAA ACCAGAAGAT
    AATCAAAATC AGGGGATCCA TCCATCAATC AGAGAAAACC AGTGGAGAAC AGGACCATGG
    GGATCATGCT CTAGCAGTTG TGCTGGGGGT GTTCAACATC GAGTTGTGGT CTGCCAGGAT
    GAAAATGGAC AAAGTGCCAG TTACTGTGAT TCAGCGTCCA AACCCCCAGA GTCAAAGCAC
    TGTGATTCGG GACCTTGTCC ACAGTGGAAC TATGGAAGCT GGGGAGAATG TACACAAACA
    TGTGGAGGAG GAATAAAATC GAGATTTGTA ATATGTCAGT TTCCCAATGG CCAACTGTCA
    CAAGAGCAAA ACTGTGAAAT GTTGAACAAG CCACCTAGTG TGGTGCAGTG TCATATACGT
    GCTTGCCCTG ATGATGTATC ATGGCATCGG GGACCATGGA AATCGTGCTC AGCTTCCTGT
    GGTAAAGGTT TAAAATACCG TGAGGTGCTT TGCATTGACC AATTCCACGG GAAATTAGAA
    GAAAAATATT GCAGTCATCT ACAGAAACCT CGAACGCATA AAGCTTGTAG GTCTGTCCAA
    TGCCCTTCAT GGAAAGCCAA CAGATGGAAG GAGTGCTCTG TGACCTGCGG CTCCGGAGTT
    CAGCAACGGG ATGTGTACTG CAGACTGAGA GGAGTTGGTC GGGTGGCGGA GGAAAAGTGT
    GATCCATCCA CACGGCCTTA TTTTCAGAGA GAGTGTTGGC ATCGGAACTG CACACAGTAC
    CACTGGGTGG CAGGAGAATG GTTGGATTGC TCAATATCGT GTAAGAAAAG AGAGACATAT
    CGGCAAGTGA AGTGCGTGGA TGCACAAAAC ATTGAAGTGA GTGAGAGTTT CTGTGACCTC
    GCAACCAGAC CTCTCGCGAT CAAAAAGTGC AGAAACTCTC CCTGCAAGTA TATCGTGATA
    ACAGGAGACT CATCCCAGTG TGCAGGTAAC TGTGGATTTA GTTATCGACA AAGGATTACA
    TATTGCATTG GGGTCCAATC TATGGAGAAA CATAAGCTTC ATGAGCTGTG GCCTATAGGC
    TATCGAGTAT GCCCTGTGCT GCCTTCCCCT CAGGTTTACA AATGCAATTT TAAGACCTGT
    TTGCATATGG CCACTTGGAA AGTTGGAAAA TGGAGCAAGT GCTCTGTGAC TTGTGGAGTT
    GGGATGATGG AGAGAAGAGT GGAATGCATG GCCGATAATG GTCTGTCCAG TGATTTATGT
    TTAAAGCATT TAAAACCAGA TGCTCAAAAG AAATGTTATG TCAGTGACTG TAAAACATTT
    ACCAGCTGTA AAGAAATACA AATGAAAAAC AACATTACCA AGGATGGTGA CTATTACCTT
    AACATTAAAG GAAGAATAAC AAAGATCTAT TGTGCTGGCA TGCACTTGCA GAACCCTAAG
    GAATATATAT CATTGGTCAA AGGTGAAGAA GACAACTTTT CTGAAGTGTA TGGTTTTAGA
    CTACGAAATC CATATGAATG TCCCTTTAAT GGAAGCAGGA GGCAAGACTG TGAATGTAAA
    AATGACTATA TGGCTGCTGG ACACACTGTT TTTAGCAAAA TAAGAATTGA TCTTACTTCC
    ATGCAAATTA AAACTACAGA CCTTCTTTTT GCCCAGACAA TATTTGGAAA TGCAGTTCCA
    TTTGCCACAG CTGGAGATTG CTATAGTGCT GCCAGATGCC CACAGGGACA GTTCAGCATT
    AATTTGGCAG GAACTGGGAT GAAGATATCC AGTACAGCAA AGTGGCTGGC TCAGGGCAGT
    TATGCCTCTG TGATCATTCA CAGATCACAG GATGGAACCA AAGTCTATGG CAGATGTGGA
    GGATTCTGTG GAAAGTGTAT TCCTCACATG ACTACTGGTC TCCCAATTCA AGTAGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847708.1
    CDS309..5828
    Translation

    Target ORF information:

    RefSeq Version XM_024991940.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991940.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGGTGG TCAAGTGGCT GACGGGGCTG CTCTACCAGC TCTCGCTCTT CATCACCAGG 
    TCTTGGGAAG TTCACTTCCA CCCCAGGCAG GAAGCCTTAG TGAGGACACT GGCCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAATGAG TTCGGCGAAG TATTCCCACA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCGGAGACT CTGGAGCCCA CACCGTTCAG AACCCACTAC
    CGAATCCGCG CCTACGGGCA GCTCTTCCAG CTGAACCTGA GCGCCGACGC GGCCTTCCTG
    GCTGCGGGAT ACACCGAGGT GCACTTAGGC ACTTCTGCGC ACCCGGCGGA GGAGCGCAGC
    GCGGCGCCCC CAGACCTACG CCACTGCTTC TACCGCGGCC AGGTCAATGC TCGCGAGGAT
    CACACAGCCG TCTTCAGCCT CTGTGGAGGC TTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGTG AACATGAAGA TGATCATAAC
    AAGCCACATC TTATATACAG ACAGGAATTA AAGAGGAACT ATTTTCTGCA GTCTCACAAG
    CCTTGTGATG TTTCAGAAAG TGAAATAAAG AAAACTACTT TACCCTTTCA TAACTACAGC
    GACGTGAATG AAAATCTCAA TATAAAGGAG GAAAGAGTTT TAGGATACAC TTCAAAAAAT
    ATATCTTTGG AAGATGAAAG AAGTCAATTG CATTCCAGGA AAAAACGTTT TTTATCATAT
    CCAAGATACG TGGAAGTTAT GGTTACAGCT GACTCTAAAA TGGTTCATCA TCACGGACAG
    AACTTGCAGC ACTATGTACT AACTCTGATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTTATAAA CATAGTAATT GTAAAATTAG TTATAATTCA TGATGAGCAG
    GAAGGACCAG TCATCAGTTT TAATGCAGCT ACCACATTAC GCAACTTCTG TTTATGGCAA
    CAAACTCAAA ATGTTCTTGA CGATGCCCAC CCATCCCACC ATGATACTGC TGTTCTTATC
    ACAAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTAGGTTT AGCAGAATTA
    GGTACCCTGT GCGATCCTTT ACGGAGCTGC TCCATTAGTG AAGAAAATGG ACTCAGTGCT
    GCCTTTACCA TAGCCCATGA GCTTGGTCAT GTATTTAATG TTCCACACGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAT TAAACATCAG TATCATGTCA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACCGGTCATG GGGAATGCCT CCTTGACAAA CCAAATGGAA GAATATATGA TCTATCTTCA
    CAGCTTCCTG GATTAATGTA TGATGTAAAC AAGCAATGTG AGCTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCCTATTT AAAACAATGC AGGCGTCTCT GGTGTACCAG TGCAGAAGGA
    GTTCACAAGG GCTGTCGCAC TCAACACATG CCACTGGCAG ATGGAACAAA CTGTGGTCCT
    GGGATGCATT GCCACCATGG GCTATGTGTA AATAAAGAAA TGGAAACACG TCCTGTAGAT
    GGCGATTGGG GACCATGGGG ACCTTACAGT TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA GCAGGCTCTG TAATCGCCCC GAGCCAAGAA ACGGAGGCAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAC ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTCCGTGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTCTCC
    CCTAACGTGA GGTGGCTTCC AAAGTACAGT GGGATTGCTG TAAAAGATCG TTGTAAACTC
    TATTGTCGGG TGGCTGGAAC CACTAGCTTC TACCAACTGA AGGACAGGGT TGCTGATGGC
    ACTCCTTGTG GAACTGAAAC AAATGACATC TGTGTTCAAG GCCTATGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAGT GTGGTGTGTG TGGTGGGGAT
    AACTCCTCCT GCAGAACAAT GGCAGGTGTC TTCAACAGTG CACATTATGG TTATAATGTC
    GTTGTAAAGA TTCCCCCAGG AGCAACAAAC ATTGATATTC TTCAGCACAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTGGCATTA TCGGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCCAAAAGG GAAATCAACG TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAACTC AATTGAAAGG ATTAATAGTA CTGATCGACT GGAAGAAGAA
    CTTGTTTTGC AGGTTTTGTG TGTGGGTAAT CTATACAACC CTGATGTACG TTATTCCTTC
    AATATCCCCG TGGAAGAGAA GAGTGATCTA TTCACATGGG ACCCATATGG ACCGTGGCAA
    GACTGTACAA AAATGTGTCA AGGTCTTCAT AGAAGAAAAA TTACTTGTGT ACGTAAGAGT
    GATCATATGG TCGTGTCTGA TCAAAGCTGT GACCACATAC CACTTCCATT GCTGGTGACT
    GAAAGGTGCA ATACAGACTG TGAACTAAGG TGGCACATCA TTGGCAAAAG TGAATGTTCA
    GCCCTTTGCG GTCAAGGATA CAAGTCACTG GACGTCCATT GTATGAAGTA TTCCATTCAT
    AACGGACAGA CTGTTCCAGT AGTTGACCAC TACTGCAGTG ACCAACTTAA GCCTCCAACC
    CGAGAACCCT GCCATGGTGA TTGTGTCTTA ACAAGGTGGC ATTATTTAGA ATGGTCTCAG
    TGCTCCAGAA GTTGTGGAGG AGGGGAGAGG TCTCGAGACT CTTACTGTAT AAATAACTTT
    GGCCACCGTC TTGCTGACAG AGAATGCCAG GAACTTCCCC GAGTGACAGT AGAGAATTGC
    AATGAATTTT CCTGTCCCAG TTGGGCCACT AGTGAGTGGA GTGAGTGTCT GGTTACCTGT
    GGAAAAGGAA CAAACCAGCG GCAGGTGTGG TGTCAACTGA ATGAAGATCA CTTGAGTGAC
    GGCTTCTGTG ACCCAAGCAC CAAGCCTGAG TCTCAGAGAC CCTGTGAGCT TCATGCGTGT
    ACTTCCTGGC AAGTAGGACC TTGGGGTTCT TGCTCTGTGT CTTGTGGAAG AGGAAATCAA
    GCCCGCTATG TAAGTTGTCG TGATGCTCAT GATGGAATAG CAGATGAATC ATTTTGTGCC
    CACTTACCCC GACCAGCTGA AATAGCTGTC TGTTTTAGCC TTTGTGGAGA GTGGCAAACA
    GGGAATTGGT CACCTTGTTC AGCTTCCTGT GGCCGTGGAA AAATGACTCG CCAAGTTTTA
    TGCATCAACT ATCATCAGCC AGTTAATGAG AATTACTGTG ACCCCGAAGT CCGCCCTGTG
    ATAGAACAAG AATGTAATCT GGCAGCCTGC CCTCCCACGT ACAGCCACTT TCCGAGTTCC
    TCTGAGCAGC CAAGCCATTT TCCAGGCAGG AATTTTCCAT TAACTCACAA ACCAGAAGAT
    AATCAAAATC AGGGGATCCA TCCATCAATC AGAGAAAACC AGTGGAGAAC AGGACCATGG
    GGATCATGCT CTAGCAGTTG TGCTGGGGGT GTTCAACATC GAGTTGTGGT CTGCCAGGAT
    GAAAATGGAC AAAGTGCCAG TTACTGTGAT TCAGCGTCCA AACCCCCAGA GTCAAAGCAC
    TGTGATTCGG GACCTTGTCC ACAGTGGAAC TATGGAAGCT GGGGAGAATG TACACAAACA
    TGTGGAGGAG GAATAAAATC GAGATTTGTA ATATGTCAGT TTCCCAATGG CCAACTGTCA
    CAAGAGCAAA ACTGTGAAAT GTTGAACAAG CCACCTAGTG TGGTGCAGTG TCATATACGT
    GCTTGCCCTG ATGATGTATC ATGGCATCGG GGACCATGGA AATCGTGCTC AGCTTCCTGT
    GGTAAAGGTT TAAAATACCG TGAGGTGCTT TGCATTGACC AATTCCACGG GAAATTAGAA
    GAAAAATATT GCAGTCATCT ACAGAAACCT CGAACGCATA AAGCTTGTAG GTCTGTCCAA
    TGCCCTTCAT GGAAAGCCAA CAGATGGAAG GAGTGCTCTG TGACCTGCGG CTCCGGAGTT
    CAGCAACGGG ATGTGTACTG CAGACTGAGA GGAGTTGGTC GGGTGGCGGA GGAAAAGTGT
    GATCCATCCA CACGGCCTTA TTTTCAGAGA GAGTGTTGGC ATCGGAACTG CACACAGTAC
    CACTGGGTGG CAGGAGAATG GTTGGATTGC TCAATATCGT GTAAGAAAAG AGAGACATAT
    CGGCAAGTGA AGTGCGTGGA TGCACAAAAC ATTGAAGTGA GTGAGAGTTT CTGTGACCTC
    GCAACCAGAC CTCTCGCGAT CAAAAAGTGC AGAAACTCTC CCTGCAAGTA TATCGTGATA
    ACAGGAGACT CATCCCAGTG TGCAGGTAAC TGTGGATTTA GTTATCGACA AAGGATTACA
    TATTGCATTG GGGTCCAATC TATGGAGAAA CATAAGCTTC ATGAGCTGTG GCCTATAGGC
    TATCGAGTAT GCCCTGTGCT GCCTTCCCCT CAGGTTTACA AATGCAATTT TAAGACCTGT
    TTGCATATGG CCACTTGGAA AGTTGGAAAA TGGAGCAAGT GCTCTGTGAC TTGTGGAGTT
    GGGATGATGG AGAGAAGAGT GGAATGCATG GCCGATAATG GTCTGTCCAG TGATTTATGT
    TTAAAGCATT TAAAACCAGA TGCTCAAAAG AAATGTTATG TCAGTGACTG TAAAACATTT
    ACCAGCTGTA AAGAAATACA AATGAAAAAC AACATTACCA AGGATGGTGA CTATTACCTT
    AACATTAAAG GAAGAATAAC AAAGATCTAT TGTGCTGGCA TGCACTTGCA GAACCCTAAG
    GAATATATAT CATTGGTCAA AGGTGAAGAA GACAACTTTT CTGAAGTGTA TGGTTTTAGA
    CTACGAAATC CATATGAATG TCCCTTTAAT GGAAGCAGGA GGCAAGACTG TGAATGTAAA
    AATGACTATA TGGCTGCTGG ACACACTGTT TTTAGCAAAA TAAGAATTGA TCTTACTTCC
    ATGCAAATTA AAACTACAGA CCTTCTTTTT GCCCAGACAA TATTTGGAAA TGCAGTTCCA
    TTTGCCACAG CTGGAGATTG CTATAGTGCT GCCAGATGCC CACAGGGACA GTTCAGCATT
    AATTTGGCAG GAACTGGGAT GAAGATATCC AGTACAGCAA AGTGGCTGGC TCAGGGCAGT
    TATGCCTCTG TGATCATTCA CAGATCACAG GATGGAACCA AAGTCTATGG CAGATGTGGA
    GGATTCTGTG GAAAGTGTAT TCCTCACATG ACTACTGGTC TCCCAATTCA AGTAGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234495
    Clone ID Related Accession (Same CDS sequence) XM_024991941.1
    Accession Version XM_024991941.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTAGGTC AGACAGGCTA CACAGATCAA CAGACTAAGC AAAGAACTAG AACTTTGGAA 
    CTCTTTTCTT TCATTTACTG TATTGGATTA TATGACAAGC ACTTCTGGCC TCTATCCCTT
    GTTATCCTAT GCATGACCAG ATTTAATGTT CCACACGATG ATAGTTTTAA ATGTAAGGAA
    ACTGGAATTA AACATCAGTA TCATGTCATG GCTCCAACTT TAAATTACCA CACAAGTCCT
    TGGACCTGGT CAAAATGTAG TCAGAAATAT ATCACTGAAT TCCTAGATAC CGGTCATGGG
    GAATGCCTCC TTGACAAACC AAATGGAAGA ATATATGATC TATCTTCACA GCTTCCTGGA
    TTAATGTATG ATGTAAACAA GCAATGTGAG CTTATGTTTG GTCCTGGATC ACAAGTGTGT
    CCCTATTTAA AACAATGCAG GCGTCTCTGG TGTACCAGTG CAGAAGGAGT TCACAAGGGC
    TGTCGCACTC AACACATGCC ACTGGCAGAT GGAACAAACT GTGGTCCTGG GATGCATTGC
    CACCATGGGC TATGTGTAAA TAAAGAAATG GAAACACGTC CTGTAGATGG CGATTGGGGA
    CCATGGGGAC CTTACAGTTC TTGTTCAAGA ACATGTGGAG GTGGAATCAA AAGCACAAGC
    AGGCTCTGTA ATCGCCCCGA GCCAAGAAAC GGAGGCAAGT ACTGTGTGGG CCGCAGGATG
    AAATTTCGAT CATGTAACAC TGATTCATGT CCAAAAGGCA AGCAAGACTT CCGTGAGAAG
    CAGTGCTCTG ATTTTGATGG TAAACATTTC AACATCAATG GTCTCTCCCC TAACGTGAGG
    TGGCTTCCAA AGTACAGTGG GATTGCTGTA AAAGATCGTT GTAAACTCTA TTGTCGGGTG
    GCTGGAACCA CTAGCTTCTA CCAACTGAAG GACAGGGTTG CTGATGGCAC TCCTTGTGGA
    ACTGAAACAA ATGACATCTG TGTTCAAGGC CTATGTCGGC AAGCTGGCTG TGATCATGTG
    TTAAACTCCA AGGCCAGGAG AGACAAGTGT GGTGTGTGTG GTGGGGATAA CTCCTCCTGC
    AGAACAATGG CAGGTGTCTT CAACAGTGCA CATTATGGTT ATAATGTCGT TGTAAAGATT
    CCCCCAGGAG CAACAAACAT TGATATTCTT CAGCACAGCT ATTCTGGAAA ACCAGAAGAT
    GACAATTACC TGGCATTATC GGACACTCAA GGGAATTTTC TTTTGAATGG GAATTTTGTT
    GTAAGTATGT CCAAAAGGGA AATCAACGTA CAAGGAGCTA TTTTTGAATA CAGTGGTTCA
    AACAACTCAA TTGAAAGGAT TAATAGTACT GATCGACTGG AAGAAGAACT TGTTTTGCAG
    GTTTTGTGTG TGGGTAATCT ATACAACCCT GATGTACGTT ATTCCTTCAA TATCCCCGTG
    GAAGAGAAGA GTGATCTATT CACATGGGAC CCATATGGAC CGTGGCAAGA CTGTACAAAA
    ATGTGTCAAG GTCTTCATAG AAGAAAAATT ACTTGTGTAC GTAAGAGTGA TCATATGGTC
    GTGTCTGATC AAAGCTGTGA CCACATACCA CTTCCATTGC TGGTGACTGA AAGGTGCAAT
    ACAGACTGTG AACTAAGGTG GCACATCATT GGCAAAAGTG AATGTTCAGC CCTTTGCGGT
    CAAGGATACA AGTCACTGGA CGTCCATTGT ATGAAGTATT CCATTCATAA CGGACAGACT
    GTTCCAGTAG TTGACCACTA CTGCAGTGAC CAACTTAAGC CTCCAACCCG AGAACCCTGC
    CATGGTGATT GTGTCTTAAC AAGGTGGCAT TATTTAGAAT GGTCTCAGTG CTCCAGAAGT
    TGTGGAGGAG GGGAGAGGTC TCGAGACTCT TACTGTATAA ATAACTTTGG CCACCGTCTT
    GCTGACAGAG AATGCCAGGA ACTTCCCCGA GTGACAGTAG AGAATTGCAA TGAATTTTCC
    TGTCCCAGTT GGGCCACTAG TGAGTGGAGT GAGTGTCTGG TTACCTGTGG AAAAGGAACA
    AACCAGCGGC AGGTGTGGTG TCAACTGAAT GAAGATCACT TGAGTGACGG CTTCTGTGAC
    CCAAGCACCA AGCCTGAGTC TCAGAGACCC TGTGAGCTTC ATGCGTGTAC TTCCTGGCAA
    GTAGGACCTT GGGGTTCTTG CACAGCCACG TGTGGACAGG GGTACCAGAT GCGTGCTGTG
    AAATGTGTCA ACGAGCTATT TAGTGCAGTG CTAGATGACA GACTGTGCCA TGGAGCTAGT
    CGCCCAAGTG AAAGGCAGGA TTGTATTGTT ATACCTTGTC CAGTTATCCC TAAAATTGGG
    GCCACTTCCT TGCCAGCTGT TCCCATGAGA AAGATAACAC AATGGCGATA TGGTTCTTGG
    ACCCCATGCT CTGTGTCTTG TGGAAGAGGA AATCAAGCCC GCTATGTAAG TTGTCGTGAT
    GCTCATGATG GAATAGCAGA TGAATCATTT TGTGCCCACT TACCCCGACC AGCTGAAATA
    GCTGTCTGTT TTAGCCTTTG TGGAGAGTGG CAAACAGGGA ATTGGTCACC TTGTTCAGCT
    TCCTGTGGCC GTGGAAAAAT GACTCGCCAA GTTTTATGCA TCAACTATCA TCAGCCAGTT
    AATGAGAATT ACTGTGACCC CGAAGTCCGC CCTGTGATAG AACAAGAATG TAATCTGGCA
    GCCTGCCCTC CCACGTACAG CCACTTTCCG AGTTCCTCTG AGCAGCCAAG CCATTTTCCA
    GGCAGGAATT TTCCATTAAC TCACAAACCA GAAGATAATC AAAATCAGGG GATCCATCCA
    TCAATCAGAG AAAACCAGTG GAGAACAGGA CCATGGGGAT CATGCTCTAG CAGTTGTGCT
    GGGGGTGTTC AACATCGAGT TGTGGTCTGC CAGGATGAAA ATGGACAAAG TGCCAGTTAC
    TGTGATTCAG CGTCCAAACC CCCAGAGTCA AAGCACTGTG ATTCGGGACC TTGTCCACAG
    TGGAACTATG GAAGCTGGGG AGAATGTACA CAAACATGTG GAGGAGGAAT AAAATCGAGA
    TTTGTAATAT GTCAGTTTCC CAATGGCCAA CTGTCACAAG AGCAAAACTG TGAAATGTTG
    AACAAGCCAC CTAGTGTGGT GCAGTGTCAT ATACGTGCTT GCCCTGATGA TGTATCATGG
    CATCGGGGAC CATGGAAATC GTGCTCAGCT TCCTGTGGTA AAGGTTTAAA ATACCGTGAG
    GTGCTTTGCA TTGACCAATT CCACGGGAAA TTAGAAGAAA AATATTGCAG TCATCTACAG
    AAACCTCGAA CGCATAAAGC TTGTAGGTCT GTCCAATGCC CTTCATGGAA AGCCAACAGA
    TGGAAGGAGT GCTCTGTGAC CTGCGGCTCC GGAGTTCAGC AACGGGATGT GTACTGCAGA
    CTGAGAGGAG TTGGTCGGGT GGCGGAGGAA AAGTGTGATC CATCCACACG GCCTTATTTT
    CAGAGAGAGT GTTGGCATCG GAACTGCACA CAGTACCACT GGGTGGCAGG AGAATGGTTG
    GATTGCTCAA TATCGTGTAA GAAAAGAGAG ACATATCGGC AAGTGAAGTG CGTGGATGCA
    CAAAACATTG AAGTGAGTGA GAGTTTCTGT GACCTCGCAA CCAGACCTCT CGCGATCAAA
    AAGTGCAGAA ACTCTCCCTG CAAGTATATC GTGATAACAG GAGACTCATC CCAGTGTGCA
    GGTAACTGTG GATTTAGTTA TCGACAAAGG ATTACATATT GCATTGGGGT CCAATCTATG
    GAGAAACATA AGCTTCATGA GCTGTGGCCT ATAGGCTATC GAGTATGCCC TGTGCTGCCT
    TCCCCTCAGG TTTACAAATG CAATTTTAAG ACCTGTTTGC ATATGGCCAC TTGGAAAGTT
    GGAAAATGGA GCAAGTGCTC TGTGACTTGT GGAGTTGGGA TGATGGAGAG AAGAGTGGAA
    TGCATGGCCG ATAATGGTCT GTCCAGTGAT TTATGTTTAA AGCATTTAAA ACCAGATGCT
    CAAAAGAAAT GTTATGTCAG TGACTGTAAA ACATTTACCA GCTGTAAAGA AATACAAATG
    AAAAACAACA TTACCAAGGA TGGTGACTAT TACCTTAACA TTAAAGGAAG AATAACAAAG
    ATCTATTGTG CTGGCATGCA CTTGCAGAAC CCTAAGGAAT ATATATCATT GGTCAAAGGT
    GAAGAAGACA ACTTTTCTGA AGTGTATGGT TTTAGACTAC GAAATCCATA TGAATGTCCC
    TTTAATGGAA GCAGGAGGCA AGACTGTGAA TGTAAAAATG ACTATATGGC TGCTGGACAC
    ACTGTTTTTA GCAAAATAAG AATTGATCTT ACTTCCATGC AAATTAAAAC TACAGACCTT
    CTTTTTGCCC AGACAATATT TGGAAATGCA GTTCCATTTG CCACAGCTGG AGATTGCTAT
    AGTGCTGCCA GATGCCCACA GGGACAGTTC AGCATTAATT TGGCAGGAAC TGGGATGAAG
    ATATCCAGTA CAGCAAAGTG GCTGGCTCAG GGCAGTTATG CCTCTGTGAT CATTCACAGA
    TCACAGGATG GAACCAAAGT CTATGGCAGA TGTGGAGGAT TCTGTGGAAA GTGTATTCCT
    CACATGACTA CTGGTCTCCC AATTCAAGTA GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847709.1
    CDS7..4662
    Translation

    Target ORF information:

    RefSeq Version XM_024991941.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991941.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTAGGTC AGACAGGCTA CACAGATCAA CAGACTAAGC AAAGAACTAG AACTTTGGAA 
    CTCTTTTCTT TCATTTACTG TATTGGATTA TATGACAAGC ACTTCTGGCC TCTATCCCTT
    GTTATCCTAT GCATGACCAG ATTTAATGTT CCACACGATG ATAGTTTTAA ATGTAAGGAA
    ACTGGAATTA AACATCAGTA TCATGTCATG GCTCCAACTT TAAATTACCA CACAAGTCCT
    TGGACCTGGT CAAAATGTAG TCAGAAATAT ATCACTGAAT TCCTAGATAC CGGTCATGGG
    GAATGCCTCC TTGACAAACC AAATGGAAGA ATATATGATC TATCTTCACA GCTTCCTGGA
    TTAATGTATG ATGTAAACAA GCAATGTGAG CTTATGTTTG GTCCTGGATC ACAAGTGTGT
    CCCTATTTAA AACAATGCAG GCGTCTCTGG TGTACCAGTG CAGAAGGAGT TCACAAGGGC
    TGTCGCACTC AACACATGCC ACTGGCAGAT GGAACAAACT GTGGTCCTGG GATGCATTGC
    CACCATGGGC TATGTGTAAA TAAAGAAATG GAAACACGTC CTGTAGATGG CGATTGGGGA
    CCATGGGGAC CTTACAGTTC TTGTTCAAGA ACATGTGGAG GTGGAATCAA AAGCACAAGC
    AGGCTCTGTA ATCGCCCCGA GCCAAGAAAC GGAGGCAAGT ACTGTGTGGG CCGCAGGATG
    AAATTTCGAT CATGTAACAC TGATTCATGT CCAAAAGGCA AGCAAGACTT CCGTGAGAAG
    CAGTGCTCTG ATTTTGATGG TAAACATTTC AACATCAATG GTCTCTCCCC TAACGTGAGG
    TGGCTTCCAA AGTACAGTGG GATTGCTGTA AAAGATCGTT GTAAACTCTA TTGTCGGGTG
    GCTGGAACCA CTAGCTTCTA CCAACTGAAG GACAGGGTTG CTGATGGCAC TCCTTGTGGA
    ACTGAAACAA ATGACATCTG TGTTCAAGGC CTATGTCGGC AAGCTGGCTG TGATCATGTG
    TTAAACTCCA AGGCCAGGAG AGACAAGTGT GGTGTGTGTG GTGGGGATAA CTCCTCCTGC
    AGAACAATGG CAGGTGTCTT CAACAGTGCA CATTATGGTT ATAATGTCGT TGTAAAGATT
    CCCCCAGGAG CAACAAACAT TGATATTCTT CAGCACAGCT ATTCTGGAAA ACCAGAAGAT
    GACAATTACC TGGCATTATC GGACACTCAA GGGAATTTTC TTTTGAATGG GAATTTTGTT
    GTAAGTATGT CCAAAAGGGA AATCAACGTA CAAGGAGCTA TTTTTGAATA CAGTGGTTCA
    AACAACTCAA TTGAAAGGAT TAATAGTACT GATCGACTGG AAGAAGAACT TGTTTTGCAG
    GTTTTGTGTG TGGGTAATCT ATACAACCCT GATGTACGTT ATTCCTTCAA TATCCCCGTG
    GAAGAGAAGA GTGATCTATT CACATGGGAC CCATATGGAC CGTGGCAAGA CTGTACAAAA
    ATGTGTCAAG GTCTTCATAG AAGAAAAATT ACTTGTGTAC GTAAGAGTGA TCATATGGTC
    GTGTCTGATC AAAGCTGTGA CCACATACCA CTTCCATTGC TGGTGACTGA AAGGTGCAAT
    ACAGACTGTG AACTAAGGTG GCACATCATT GGCAAAAGTG AATGTTCAGC CCTTTGCGGT
    CAAGGATACA AGTCACTGGA CGTCCATTGT ATGAAGTATT CCATTCATAA CGGACAGACT
    GTTCCAGTAG TTGACCACTA CTGCAGTGAC CAACTTAAGC CTCCAACCCG AGAACCCTGC
    CATGGTGATT GTGTCTTAAC AAGGTGGCAT TATTTAGAAT GGTCTCAGTG CTCCAGAAGT
    TGTGGAGGAG GGGAGAGGTC TCGAGACTCT TACTGTATAA ATAACTTTGG CCACCGTCTT
    GCTGACAGAG AATGCCAGGA ACTTCCCCGA GTGACAGTAG AGAATTGCAA TGAATTTTCC
    TGTCCCAGTT GGGCCACTAG TGAGTGGAGT GAGTGTCTGG TTACCTGTGG AAAAGGAACA
    AACCAGCGGC AGGTGTGGTG TCAACTGAAT GAAGATCACT TGAGTGACGG CTTCTGTGAC
    CCAAGCACCA AGCCTGAGTC TCAGAGACCC TGTGAGCTTC ATGCGTGTAC TTCCTGGCAA
    GTAGGACCTT GGGGTTCTTG CACAGCCACG TGTGGACAGG GGTACCAGAT GCGTGCTGTG
    AAATGTGTCA ACGAGCTATT TAGTGCAGTG CTAGATGACA GACTGTGCCA TGGAGCTAGT
    CGCCCAAGTG AAAGGCAGGA TTGTATTGTT ATACCTTGTC CAGTTATCCC TAAAATTGGG
    GCCACTTCCT TGCCAGCTGT TCCCATGAGA AAGATAACAC AATGGCGATA TGGTTCTTGG
    ACCCCATGCT CTGTGTCTTG TGGAAGAGGA AATCAAGCCC GCTATGTAAG TTGTCGTGAT
    GCTCATGATG GAATAGCAGA TGAATCATTT TGTGCCCACT TACCCCGACC AGCTGAAATA
    GCTGTCTGTT TTAGCCTTTG TGGAGAGTGG CAAACAGGGA ATTGGTCACC TTGTTCAGCT
    TCCTGTGGCC GTGGAAAAAT GACTCGCCAA GTTTTATGCA TCAACTATCA TCAGCCAGTT
    AATGAGAATT ACTGTGACCC CGAAGTCCGC CCTGTGATAG AACAAGAATG TAATCTGGCA
    GCCTGCCCTC CCACGTACAG CCACTTTCCG AGTTCCTCTG AGCAGCCAAG CCATTTTCCA
    GGCAGGAATT TTCCATTAAC TCACAAACCA GAAGATAATC AAAATCAGGG GATCCATCCA
    TCAATCAGAG AAAACCAGTG GAGAACAGGA CCATGGGGAT CATGCTCTAG CAGTTGTGCT
    GGGGGTGTTC AACATCGAGT TGTGGTCTGC CAGGATGAAA ATGGACAAAG TGCCAGTTAC
    TGTGATTCAG CGTCCAAACC CCCAGAGTCA AAGCACTGTG ATTCGGGACC TTGTCCACAG
    TGGAACTATG GAAGCTGGGG AGAATGTACA CAAACATGTG GAGGAGGAAT AAAATCGAGA
    TTTGTAATAT GTCAGTTTCC CAATGGCCAA CTGTCACAAG AGCAAAACTG TGAAATGTTG
    AACAAGCCAC CTAGTGTGGT GCAGTGTCAT ATACGTGCTT GCCCTGATGA TGTATCATGG
    CATCGGGGAC CATGGAAATC GTGCTCAGCT TCCTGTGGTA AAGGTTTAAA ATACCGTGAG
    GTGCTTTGCA TTGACCAATT CCACGGGAAA TTAGAAGAAA AATATTGCAG TCATCTACAG
    AAACCTCGAA CGCATAAAGC TTGTAGGTCT GTCCAATGCC CTTCATGGAA AGCCAACAGA
    TGGAAGGAGT GCTCTGTGAC CTGCGGCTCC GGAGTTCAGC AACGGGATGT GTACTGCAGA
    CTGAGAGGAG TTGGTCGGGT GGCGGAGGAA AAGTGTGATC CATCCACACG GCCTTATTTT
    CAGAGAGAGT GTTGGCATCG GAACTGCACA CAGTACCACT GGGTGGCAGG AGAATGGTTG
    GATTGCTCAA TATCGTGTAA GAAAAGAGAG ACATATCGGC AAGTGAAGTG CGTGGATGCA
    CAAAACATTG AAGTGAGTGA GAGTTTCTGT GACCTCGCAA CCAGACCTCT CGCGATCAAA
    AAGTGCAGAA ACTCTCCCTG CAAGTATATC GTGATAACAG GAGACTCATC CCAGTGTGCA
    GGTAACTGTG GATTTAGTTA TCGACAAAGG ATTACATATT GCATTGGGGT CCAATCTATG
    GAGAAACATA AGCTTCATGA GCTGTGGCCT ATAGGCTATC GAGTATGCCC TGTGCTGCCT
    TCCCCTCAGG TTTACAAATG CAATTTTAAG ACCTGTTTGC ATATGGCCAC TTGGAAAGTT
    GGAAAATGGA GCAAGTGCTC TGTGACTTGT GGAGTTGGGA TGATGGAGAG AAGAGTGGAA
    TGCATGGCCG ATAATGGTCT GTCCAGTGAT TTATGTTTAA AGCATTTAAA ACCAGATGCT
    CAAAAGAAAT GTTATGTCAG TGACTGTAAA ACATTTACCA GCTGTAAAGA AATACAAATG
    AAAAACAACA TTACCAAGGA TGGTGACTAT TACCTTAACA TTAAAGGAAG AATAACAAAG
    ATCTATTGTG CTGGCATGCA CTTGCAGAAC CCTAAGGAAT ATATATCATT GGTCAAAGGT
    GAAGAAGACA ACTTTTCTGA AGTGTATGGT TTTAGACTAC GAAATCCATA TGAATGTCCC
    TTTAATGGAA GCAGGAGGCA AGACTGTGAA TGTAAAAATG ACTATATGGC TGCTGGACAC
    ACTGTTTTTA GCAAAATAAG AATTGATCTT ACTTCCATGC AAATTAAAAC TACAGACCTT
    CTTTTTGCCC AGACAATATT TGGAAATGCA GTTCCATTTG CCACAGCTGG AGATTGCTAT
    AGTGCTGCCA GATGCCCACA GGGACAGTTC AGCATTAATT TGGCAGGAAC TGGGATGAAG
    ATATCCAGTA CAGCAAAGTG GCTGGCTCAG GGCAGTTATG CCTCTGTGAT CATTCACAGA
    TCACAGGATG GAACCAAAGT CTATGGCAGA TGTGGAGGAT TCTGTGGAAA GTGTATTCCT
    CACATGACTA CTGGTCTCCC AATTCAAGTA GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19305
    Clone ID Related Accession (Same CDS sequence) NM_001206093.1
    Accession Version NM_001206093.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02013044.1, DAAA02013048.1, DAAA02013049.1, DAAA02013051.1, DAAA02013052.1, DAAA02013053.1, DAAA02013060.1 and DAAA02013061.1. On Apr 20, 2011 this sequence version replaced XM_616258.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN04019636, SAMN04019639 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCGGGTGG TCAAGTGGCT GACGGGGCTG CTCTACCAGC TCTCGCTCTT CATCACCAGG 
    TCTTGGGAAG TTCACTTCCA CCCCAGGCAG GAAGCCTTAG TGAGGACACT GGCCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAATGAG TTCGGCGAAG TATTCCCACA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCGGAGACT CTGGAGCCCA CACCGTTCAG AACCCACTAC
    CGAATCCGCG CCTACGGGCA GCTCTTCCAG CTGAACCTGA GCGCCGACGC GGCCTTCCTG
    GCTGCGGGAT ACACCGAGGT GCACTTAGGC ACTTCTGCGC ACCCGGCGGA GGAGCGCAGC
    GCGGCGCCCC CAGACCTACG CCACTGCTTC TACCGCGGCC AGGTCAATGC TCGCGAGGAT
    CACACAGCCG TCTTCAGCCT CTGTGGAGGC TTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGTG AACATGAAGA TGATCATAAC
    AAGCCACATC TTATATACAG ACAGGAATTA AAGAGGAACT ATTTTCTGCA GTCTCACAAG
    CCTTGTGATG TTTCAGAAAG TGAAATAAAG AAAACTACTT TACCCTTTCA TAACTACAGC
    GACGTGAATG AAAATCTCAA TATAAAGGAG GAAAGAGTTT TAGGATACAC TTCAAAAAAT
    ATATCTTTGG AAGATGAAAG AAGTCAATTG CATTCCAGGA AAAAACGTTT TTTATCATAT
    CCAAGATACG TGGAAGTTAT GGTTACAGCT GACTCTAAAA TGGTTCATCA TCACGGACAG
    AACTTGCAGC ACTATGTACT AACTCTGATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTTATAAA CATAGTAATT GTAAAATTAG TTATAATTCA TGATGAGCAG
    GAAGGACCAG TCATCAGTTT TAATGCAGCT ACCACATTAC GCAACTTCTG TTTATGGCAA
    CAAACTCAAA ATGTTCTTGA CGATGCCCAC CCATCCCACC ATGATACTGC TGTTCTTATC
    ACAAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTAGGTTT AGCAGAATTA
    GGTACCCTGT GCGATCCTTT ACGGAGCTGC TCCATTAGTG AAGAAAATGG ACTCAGTGCT
    GCCTTTACCA TAGCCCATGA GCTTGGTCAT GTATTTAATG TTCCACACGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAT TAAACATCAG TATCATGTCA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACCGGTCATG GGGAATGCCT CCTTGACAAA CCAAATGGAA GAATATATGA TCTATCTTCA
    CAGCTTCCTG GATTAATGTA TGATGTAAAC AAGCAATGTG AGCTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCCTATTT AAAACAATGC AGGCGTCTCT GGTGTACCAG TGCAGAAGGA
    GTTCACAAGG GCTGTCGCAC TCAACACATG CCACTGGCAG ATGGAACAAA CTGTGGTCCT
    GGGATGCATT GCCACCATGG GCTATGTGTA AATAAAGAAA TGGAAACACG TCCTGTAGAT
    GGCGATTGGG GACCATGGGG ACCTTACAGT TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA GCAGGCTCTG TAATCGCCCC GAGCCAAGAA ACGGAGGCAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAC ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTCCGTGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTCTCC
    CCTAACGTGA GGTGGCTTCC AAAGTACAGT GGGATTGCTG TAAAAGATCG TTGTAAACTC
    TATTGTCGGG TGGCTGGAAC CACTAGCTTC TACCAACTGA AGGACAGGGT TGCCGATGGC
    ACTCCTTGTG GAACTGAAAC AAATGACATC TGTGTTCAAG GCCTATGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAGT GTGGTGTGTG TGGTGGGGAT
    AACTCCTCCT GCAGAACAAT GGCAGGTGTC TTCAACAGTG CACATTATGG TTATAATGTC
    GTTGTAAAGA TTCCCCCAGG AGCAACAAAC ATTGATATTC TTCAGCACAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTGGCATTA TCGGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCCAAAAGG GAAATCAACG TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAACTC AATTGAAAGG ATTAATAGTA CTGATCGACT GGAAGAAGAA
    CTTGTTTTGC AGGTTTTGTG TGTGGGTAAT CTATACAACC CTGATGTACG TTATTCCTTC
    AATATCCCCG TGGAAGAGAA GAGTGATCTA TTCACATGGG ACCCATATGG ACCGTGGCAA
    GACTGTACAA AAATGTGTCA AGGTCTTCAT AGAAGAAAAA TTACTTGTGT ACGTAAGAGT
    GATCATATGG TCGTGTCTGA TCAAAGCTGT GACCACATAC CACTTCCATT GCTGGTGACT
    GAAAGGTGCA ATACAGACTG TGAACTAAGG TGGCACATCA TTGGCAAAAG TGAATGTTCA
    GCCCTTTGCG GTCAAGGATA CAAGTCACTG GACGTCCATT GTATGAAGTA TTCCATTCAT
    AACGGACAGA CTGTTCCAGT AGTTGACCAC TACTGCAGTG ACCAACTTAA GCCTCCAACC
    CGAGAACCCT GCCATGGTGA TTGTGTCTTA ACAAGGTGGC ATTATTTAGA ATGGTCTCAG
    TGCTCCAGAA GTTGTGGAGG AGGGGAGAGG TCTCGAGACT CTTACTGTAT AAATAACTTT
    GGCCACCGTC TTGCTGACAG AGAATGCCAG GAACTTCCCC GAGTGACAGT AGAGAATTGC
    AATGAATTTT CCTGTCCCAG TTGGGCCACT AGTGAGTGGA GTGAGTGTCT GGTTACCTGT
    GGAAAAGGAA CAAACCAGCG GCAGGTGTGG TGTCAACTGA ATGAAGATCA CTTGAGTGAC
    GGCTTCTGTG ACCCAAGCAC CAAGCCTGAG TCTCAGAGAC CCTGTGAGCT TCATGCGTGT
    ACTTCCTGGC AAGTAGGACC TTGGGGTTCT TGCACAGCCA CGTGTGGACA GGGGTACCAG
    ATGCGTGCTG TGAAATGTGT CAACGAGCTA TTTAGTGCAG TGCTAGATGA CAGACTGTGC
    CATGGAGCTA GTCGCCCAAG TGAAAGGCAG GATTGTATTG TTATACCTTG TCCAGTTATC
    CCTAAAATTG GGGCCACTTC CTTGCCAGCT GTTCCCATGA GAAAGATAAC ACAATGGCGA
    TATGGTTCTT GGACCCCATG CTCTGTGTCT TGTGGAAGAG GAAATCAAGC CCGCTATGTA
    AGTTGTCGTG ATGCTCATGA TGGAATAGCA GATGAATCAT TTTGTGCCCA CTTACCCCGA
    CCAGCTGAAA TAGCTGTCTG TTTTAGCCTT TGTGGAGAGT GGCAAACAGG GAATTGGTCA
    CCTTGTTCAG CTTCCTGTGG CCGTGGAAAA ATGACTCGCC AAGTTTTATG CATCAACTAT
    CATCAGCCAG TTAATGAGAA TTACTGTGAC CCCGAAGTCC GCCCTGTGAT AGAACAAGAA
    TGTAATCTGG CAGCCTGCCC TCCCACGTAC AGCCACTTTC CGAGTTCCTC TGAGCAGCCA
    AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAAGATAA TCAAAATCAG
    GGGATCCATC CATCAATCAG AGAAAACCAG TGGAGAACAG GACCATGGGG ATCATGCTCT
    AGCAGTTGTG CTGGGGGTGT TCAACATCGA GTTGTGGTCT GCCAGGATGA AAATGGACAA
    AGTGCCAGTT ACTGTGATTC AGCGTCCAAA CCCCCAGAGT CAAAGCACTG TGATTCGGGA
    CCTTGTCCAC AGTGGAACTA TGGAAGCTGG GGAGAATGTA CACAAACATG TGGAGGAGGA
    ATAAAATCGA GATTTGTAAT ATGTCAGTTT CCCAATGGCC AACTGTCACA AGAGCAAAAC
    TGTGAAATGT TGAACAAGCC ACCTAGTGTG GTGCAGTGTC ATATACGTGC TTGCCCTGAT
    GATGTATCAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCCTGTGG TAAAGGTTTA
    AAATACCGTG AGGTGCTTTG CATTGACCAA TTCCACGGGA AATTAGAAGA AAAATATTGC
    AGTCATCTAC AGAAACCTCG AACGCATAAA GCTTGTAGGT CTGTCCAATG CCCTTCATGG
    AAAGCCAACA GATGGAAGGA GTGCTCTGTG ACCTGCGGCT CCGGAGTTCA GCAACGGGAT
    GTGTACTGCA GACTGAGAGG AGTTGGTCGG GTGGCGGAGG AAAAGTGTGA TCCATCCACA
    CGGCCTTATT TTCAGAGAGA GTGTTGGCAT CGGAACTGCA CACAGTACCA CTGGGTGGCA
    GGAGAATGGT TGGATTGCTC AATATCGTGT AAGAAAAGAG AGACATATCG GCAAGTGAAG
    TGCGTGGATG CACAAAACAT TGAAGTGAGT GAGAGTTTCT GTGACCTCGC AACCAGACCT
    CTCGCGATCA AAAAGTGCAG AAACTCTCCC TGCAAGTATA TCGTGATAAC AGGAGACTCA
    TCCCAGTGTG CAGGTAACTG TGGATTTAGT TATCGACAAA GGATTACATA TTGCATTGGG
    GTCCAATCTA TGGAGAAACA TAAGCTTCAT GAGCTGTGGC CTATAGGCTA TCGAGTATGC
    CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA AGACCTGTTT GCATATGGCC
    ACTTGGAAAG TTGGAAAATG GAGCAAGTGC TCTGTGACTT GTGGAGTTGG GATGATGGAG
    AGAAGAGTGG AATGCATGGC CGATAATGGT CTGTCCAGTG ATTTATGTTT AAAGCATTTA
    AAACCAGATG CTCAAAAGAA ATGTTATGTC AGTGACTGTA AAACATTTAC CAGCTGTAAA
    GAAATACAAA TGAAAAACAA CATTACCAAG GATGGTGACT ATTACCTTAA CATTAAAGGA
    AGAATAACAA AGATCTATTG TGCTGGCATG CACTTGCAGA ACCCTAAGGA ATATATATCA
    TTGGTCAAAG GTGAAGAAGA CAACTTTTCT GAAGTGTATG GTTTTAGACT ACGAAATCCA
    TATGAATGTC CCTTTAATGG AAGCAGGAGG CAAGACTGTG AATGTAAAAA TGACTATATG
    GCTGCTGGAC ACACTGTTTT TAGCAAAATA AGAATTGATC TTACTTCCAT GCAAATTAAA
    ACTACAGACC TTCTTTTTGC CCAGACAATA TTTGGAAATG CAGTTCCATT TGCCACAGCT
    GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGACAGT TCAGCATTAA TTTGGCAGGA
    ACTGGGATGA AGATATCCAG TACAGCAAAG TGGCTGGCTC AGGGCAGTTA TGCCTCTGTG
    ATCATTCACA GATCACAGGA TGGAACCAAA GTCTATGGCA GATGTGGAGG ATTCTGTGGA
    AAGTGTATTC CTCACATGAC TACTGGTCTC CCAATTCAAG TAGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193022.1
    CDS1..5748
    Translation

    Target ORF information:

    RefSeq Version NM_001206093.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206093.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCGGGTGG TCAAGTGGCT GACGGGGCTG CTCTACCAGC TCTCGCTCTT CATCACCAGG 
    TCTTGGGAAG TTCACTTCCA CCCCAGGCAG GAAGCCTTAG TGAGGACACT GGCCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAATGAG TTCGGCGAAG TATTCCCACA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCGGAGACT CTGGAGCCCA CACCGTTCAG AACCCACTAC
    CGAATCCGCG CCTACGGGCA GCTCTTCCAG CTGAACCTGA GCGCCGACGC GGCCTTCCTG
    GCTGCGGGAT ACACCGAGGT GCACTTAGGC ACTTCTGCGC ACCCGGCGGA GGAGCGCAGC
    GCGGCGCCCC CAGACCTACG CCACTGCTTC TACCGCGGCC AGGTCAATGC TCGCGAGGAT
    CACACAGCCG TCTTCAGCCT CTGTGGAGGC TTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGTG AACATGAAGA TGATCATAAC
    AAGCCACATC TTATATACAG ACAGGAATTA AAGAGGAACT ATTTTCTGCA GTCTCACAAG
    CCTTGTGATG TTTCAGAAAG TGAAATAAAG AAAACTACTT TACCCTTTCA TAACTACAGC
    GACGTGAATG AAAATCTCAA TATAAAGGAG GAAAGAGTTT TAGGATACAC TTCAAAAAAT
    ATATCTTTGG AAGATGAAAG AAGTCAATTG CATTCCAGGA AAAAACGTTT TTTATCATAT
    CCAAGATACG TGGAAGTTAT GGTTACAGCT GACTCTAAAA TGGTTCATCA TCACGGACAG
    AACTTGCAGC ACTATGTACT AACTCTGATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTTATAAA CATAGTAATT GTAAAATTAG TTATAATTCA TGATGAGCAG
    GAAGGACCAG TCATCAGTTT TAATGCAGCT ACCACATTAC GCAACTTCTG TTTATGGCAA
    CAAACTCAAA ATGTTCTTGA CGATGCCCAC CCATCCCACC ATGATACTGC TGTTCTTATC
    ACAAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTAGGTTT AGCAGAATTA
    GGTACCCTGT GCGATCCTTT ACGGAGCTGC TCCATTAGTG AAGAAAATGG ACTCAGTGCT
    GCCTTTACCA TAGCCCATGA GCTTGGTCAT GTATTTAATG TTCCACACGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAT TAAACATCAG TATCATGTCA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACCGGTCATG GGGAATGCCT CCTTGACAAA CCAAATGGAA GAATATATGA TCTATCTTCA
    CAGCTTCCTG GATTAATGTA TGATGTAAAC AAGCAATGTG AGCTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCCTATTT AAAACAATGC AGGCGTCTCT GGTGTACCAG TGCAGAAGGA
    GTTCACAAGG GCTGTCGCAC TCAACACATG CCACTGGCAG ATGGAACAAA CTGTGGTCCT
    GGGATGCATT GCCACCATGG GCTATGTGTA AATAAAGAAA TGGAAACACG TCCTGTAGAT
    GGCGATTGGG GACCATGGGG ACCTTACAGT TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA GCAGGCTCTG TAATCGCCCC GAGCCAAGAA ACGGAGGCAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAC ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTCCGTGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTCTCC
    CCTAACGTGA GGTGGCTTCC AAAGTACAGT GGGATTGCTG TAAAAGATCG TTGTAAACTC
    TATTGTCGGG TGGCTGGAAC CACTAGCTTC TACCAACTGA AGGACAGGGT TGCCGATGGC
    ACTCCTTGTG GAACTGAAAC AAATGACATC TGTGTTCAAG GCCTATGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAGT GTGGTGTGTG TGGTGGGGAT
    AACTCCTCCT GCAGAACAAT GGCAGGTGTC TTCAACAGTG CACATTATGG TTATAATGTC
    GTTGTAAAGA TTCCCCCAGG AGCAACAAAC ATTGATATTC TTCAGCACAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTGGCATTA TCGGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCCAAAAGG GAAATCAACG TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAACTC AATTGAAAGG ATTAATAGTA CTGATCGACT GGAAGAAGAA
    CTTGTTTTGC AGGTTTTGTG TGTGGGTAAT CTATACAACC CTGATGTACG TTATTCCTTC
    AATATCCCCG TGGAAGAGAA GAGTGATCTA TTCACATGGG ACCCATATGG ACCGTGGCAA
    GACTGTACAA AAATGTGTCA AGGTCTTCAT AGAAGAAAAA TTACTTGTGT ACGTAAGAGT
    GATCATATGG TCGTGTCTGA TCAAAGCTGT GACCACATAC CACTTCCATT GCTGGTGACT
    GAAAGGTGCA ATACAGACTG TGAACTAAGG TGGCACATCA TTGGCAAAAG TGAATGTTCA
    GCCCTTTGCG GTCAAGGATA CAAGTCACTG GACGTCCATT GTATGAAGTA TTCCATTCAT
    AACGGACAGA CTGTTCCAGT AGTTGACCAC TACTGCAGTG ACCAACTTAA GCCTCCAACC
    CGAGAACCCT GCCATGGTGA TTGTGTCTTA ACAAGGTGGC ATTATTTAGA ATGGTCTCAG
    TGCTCCAGAA GTTGTGGAGG AGGGGAGAGG TCTCGAGACT CTTACTGTAT AAATAACTTT
    GGCCACCGTC TTGCTGACAG AGAATGCCAG GAACTTCCCC GAGTGACAGT AGAGAATTGC
    AATGAATTTT CCTGTCCCAG TTGGGCCACT AGTGAGTGGA GTGAGTGTCT GGTTACCTGT
    GGAAAAGGAA CAAACCAGCG GCAGGTGTGG TGTCAACTGA ATGAAGATCA CTTGAGTGAC
    GGCTTCTGTG ACCCAAGCAC CAAGCCTGAG TCTCAGAGAC CCTGTGAGCT TCATGCGTGT
    ACTTCCTGGC AAGTAGGACC TTGGGGTTCT TGCACAGCCA CGTGTGGACA GGGGTACCAG
    ATGCGTGCTG TGAAATGTGT CAACGAGCTA TTTAGTGCAG TGCTAGATGA CAGACTGTGC
    CATGGAGCTA GTCGCCCAAG TGAAAGGCAG GATTGTATTG TTATACCTTG TCCAGTTATC
    CCTAAAATTG GGGCCACTTC CTTGCCAGCT GTTCCCATGA GAAAGATAAC ACAATGGCGA
    TATGGTTCTT GGACCCCATG CTCTGTGTCT TGTGGAAGAG GAAATCAAGC CCGCTATGTA
    AGTTGTCGTG ATGCTCATGA TGGAATAGCA GATGAATCAT TTTGTGCCCA CTTACCCCGA
    CCAGCTGAAA TAGCTGTCTG TTTTAGCCTT TGTGGAGAGT GGCAAACAGG GAATTGGTCA
    CCTTGTTCAG CTTCCTGTGG CCGTGGAAAA ATGACTCGCC AAGTTTTATG CATCAACTAT
    CATCAGCCAG TTAATGAGAA TTACTGTGAC CCCGAAGTCC GCCCTGTGAT AGAACAAGAA
    TGTAATCTGG CAGCCTGCCC TCCCACGTAC AGCCACTTTC CGAGTTCCTC TGAGCAGCCA
    AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAAGATAA TCAAAATCAG
    GGGATCCATC CATCAATCAG AGAAAACCAG TGGAGAACAG GACCATGGGG ATCATGCTCT
    AGCAGTTGTG CTGGGGGTGT TCAACATCGA GTTGTGGTCT GCCAGGATGA AAATGGACAA
    AGTGCCAGTT ACTGTGATTC AGCGTCCAAA CCCCCAGAGT CAAAGCACTG TGATTCGGGA
    CCTTGTCCAC AGTGGAACTA TGGAAGCTGG GGAGAATGTA CACAAACATG TGGAGGAGGA
    ATAAAATCGA GATTTGTAAT ATGTCAGTTT CCCAATGGCC AACTGTCACA AGAGCAAAAC
    TGTGAAATGT TGAACAAGCC ACCTAGTGTG GTGCAGTGTC ATATACGTGC TTGCCCTGAT
    GATGTATCAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCCTGTGG TAAAGGTTTA
    AAATACCGTG AGGTGCTTTG CATTGACCAA TTCCACGGGA AATTAGAAGA AAAATATTGC
    AGTCATCTAC AGAAACCTCG AACGCATAAA GCTTGTAGGT CTGTCCAATG CCCTTCATGG
    AAAGCCAACA GATGGAAGGA GTGCTCTGTG ACCTGCGGCT CCGGAGTTCA GCAACGGGAT
    GTGTACTGCA GACTGAGAGG AGTTGGTCGG GTGGCGGAGG AAAAGTGTGA TCCATCCACA
    CGGCCTTATT TTCAGAGAGA GTGTTGGCAT CGGAACTGCA CACAGTACCA CTGGGTGGCA
    GGAGAATGGT TGGATTGCTC AATATCGTGT AAGAAAAGAG AGACATATCG GCAAGTGAAG
    TGCGTGGATG CACAAAACAT TGAAGTGAGT GAGAGTTTCT GTGACCTCGC AACCAGACCT
    CTCGCGATCA AAAAGTGCAG AAACTCTCCC TGCAAGTATA TCGTGATAAC AGGAGACTCA
    TCCCAGTGTG CAGGTAACTG TGGATTTAGT TATCGACAAA GGATTACATA TTGCATTGGG
    GTCCAATCTA TGGAGAAACA TAAGCTTCAT GAGCTGTGGC CTATAGGCTA TCGAGTATGC
    CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA AGACCTGTTT GCATATGGCC
    ACTTGGAAAG TTGGAAAATG GAGCAAGTGC TCTGTGACTT GTGGAGTTGG GATGATGGAG
    AGAAGAGTGG AATGCATGGC CGATAATGGT CTGTCCAGTG ATTTATGTTT AAAGCATTTA
    AAACCAGATG CTCAAAAGAA ATGTTATGTC AGTGACTGTA AAACATTTAC CAGCTGTAAA
    GAAATACAAA TGAAAAACAA CATTACCAAG GATGGTGACT ATTACCTTAA CATTAAAGGA
    AGAATAACAA AGATCTATTG TGCTGGCATG CACTTGCAGA ACCCTAAGGA ATATATATCA
    TTGGTCAAAG GTGAAGAAGA CAACTTTTCT GAAGTGTATG GTTTTAGACT ACGAAATCCA
    TATGAATGTC CCTTTAATGG AAGCAGGAGG CAAGACTGTG AATGTAAAAA TGACTATATG
    GCTGCTGGAC ACACTGTTTT TAGCAAAATA AGAATTGATC TTACTTCCAT GCAAATTAAA
    ACTACAGACC TTCTTTTTGC CCAGACAATA TTTGGAAATG CAGTTCCATT TGCCACAGCT
    GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGACAGT TCAGCATTAA TTTGGCAGGA
    ACTGGGATGA AGATATCCAG TACAGCAAAG TGGCTGGCTC AGGGCAGTTA TGCCTCTGTG
    ATCATTCACA GATCACAGGA TGGAACCAAA GTCTATGGCA GATGTGGAGG ATTCTGTGGA
    AAGTGTATTC CTCACATGAC TACTGGTCTC CCAATTCAAG TAGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53323
    Clone ID Related Accession (Same CDS sequence) XM_015471029.1
    Accession Version XM_015471029.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103922.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGGTGG TCAAGTGGCT GACGGGGCTG CTCTACCAGC TCTCGCTCTT CATCACCAGG 
    TCTTGGGAAG TTCACTTCCA CCCCAGGCAG GAAGCCTTAG TGAGGACACT GGCCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAATGAG TTCGGCGAAG TATTCCCACA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCGGAGACT CTGGAGCCCA CACCGTTCAG AACCCACTAC
    CGAATCCGCG CCTACGGGCA GCTCTTCCAG CTGAACCTGA GCGCCGACGC GGCCTTCCTG
    GCTGCGGGAT ACACCGAGGT GCACTTAGGC ACTTCTGCGC ACCCGGCGGA GGAGCGCAGC
    GCGGCGCCCC CAGACCTACG CCACTGCTTC TACCGCGGCC AGGTCAATGC TCGCGAGGAT
    CACACAGCCG TCTTCAGCCT CTGTGGAGGC TTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGTG AACATGAAGA TGATCATAAC
    AAGCCACATC TTATATACAG ACAGGAATTA AAGAGGAACT ATTTTCTGCA GTCTCACAAG
    CCTTGTGATG TTTCAGAAAG TGAAATAAAG AAAACTACTT TACCCTTTCA TAACTACAGC
    GACGTGAATG AAAATCTCAA TATAAAGGAG GAAAGAGTTT TAGGATACAC TTCAAAAAAT
    ATATCTTTGG AAGATGAAAG AAGTCAATTG CATTCCAGGA AAAAACGTTT TTTATCATAT
    CCAAGATACG TGGAAGTTAT GGTTACAGCT GACTCTAAAA TGGTTCATCA TCACGGACAG
    AACTTGCAGC ACTATGTACT AACTCTGATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTTATAAA CATAGTAATT GTAAAATTAG TTATAATTCA TGATGAGCAG
    GAAGGACCAG TCATCAGTTT TAATGCAGCT ACCACATTAC GCAACTTCTG TTTATGGCAA
    CAAACTCAAA ATGTTCTTGA CGATGCCCAC CCATCCCACC ATGATACTGC TGTTCTTATC
    ACAAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTAGGTTT AGCAGAATTA
    GGTACCCTGT GCGATCCTTT ACGGAGCTGC TCCATTAGTG AAGAAAATGG ACTCAGTGCT
    GCCTTTACCA TAGCCCATGA GCTTGGTCAT GTATTTAATG TTCCACACGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAT TAAACATCAG TATCATGTCA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACCGGTCATG GGGAATGCCT CCTTGACAAA CCAAATGGAA GAATATATGA TCTATCTTCA
    CAGCTTCCTG GATTAATGTA TGATGTAAAC AAGCAATGTG AGCTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCCTATTT AAAACAATGC AGGCGTCTCT GGTGTACCAG TGCAGAAGGA
    GTTCACAAGG GCTGTCGCAC TCAACACATG CCACTGGCAG ATGGAACAAA CTGTGGTCCT
    GGGATGCATT GCCACCATGG GCTATGTGTA AATAAAGAAA TGGAAACACG TCCTGTAGAT
    GGCGATTGGG GACCATGGGG ACCTTACAGT TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA GCAGGCTCTG TAATCGCCCC GAGCCAAGAA ACGGAGGCAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAC ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTCCGTGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTCTCC
    CCTAACGTGA GGTGGCTTCC AAAGTACAGT GGGATTGCTG TAAAAGATCG TTGTAAACTC
    TATTGTCGGG TGGCTGGAAC CACTAGCTTC TACCAACTGA AGGACAGGGT TGCCGATGGC
    ACTCCTTGTG GAACTGAAAC AAATGACATC TGTGTTCAAG GCCTATGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAGT GTGGTGTGTG TGGTGGGGAT
    AACTCCTCCT GCAGAACAAT GGCAGGTGTC TTCAACAGTG CACATTATGG TTATAATGTC
    GTTGTAAAGA TTCCCCCAGG AGCAACAAAC ATTGATATTC TTCAGCACAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTGGCATTA TCGGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCCAAAAGG GAAATCAACG TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAACTC AATTGAAAGG ATTAATAGTA CTGATCGACT GGAAGAAGAA
    CTTGTTTTGC AGGTTTTGTG TGTGGGTAAT CTATACAACC CTGATGTACG TTATTCCTTC
    AATATCCCCG TGGAAGAGAA GAGTGATCTA TTCACATGGG ACCCATATGG ACCGTGGCAA
    GACTGTACAA AAATGTGTCA AGGTCTTCAT AGAAGAAAAA TTACTTGTGT ACGTAAGAGT
    GATCATATGG TCGTGTCTGA TCAAAGCTGT GACCACATAC CACTTCCATT GCTGGTGACT
    GAAAGGTGCA ATACAGACTG TGAACTAAGG TGGCACATCA TTGGCAAAAG TGAATGTTCA
    GCCCTTTGCG GTCAAGGATA CAAGTCACTG GACGTCCATT GTATGAAGTA TTCCATTCAT
    AACGGACAGA CTGTTCCAGT AGTTGACCAC TACTGCAGTG ACCAACTTAA GCCTCCAACC
    CGAGAACCCT GCCATGGTGA TTGTGTCTTA ACAAGGTGGC ATTATTTAGA ATGGTCTCAG
    TGCTCCAGAA GTTGTGGAGG AGGGGAGAGG TCTCGAGACT CTTACTGTAT AAATAACTTT
    GGCCACCGTC TTGCTGACAG AGAATGCCAG GAACTTCCCC GAGTGACAGT AGAGAATTGC
    AATGAATTTT CCTGTCCCAG TTGGGCCACT AGTGAGTGGA GTGAGTGTCT GGTTACCTGT
    GGAAAAGGAA CAAACCAGCG GCAGGTGTGG TGTCAACTGA ATGAAGATCA CTTGAGTGAC
    GGCTTCTGTG ACCCAAGCAC CAAGCCTGAG TCTCAGAGAC CCTGTGAGCT TCATGCGTGT
    ACTTCCTGGC AAGTAGGACC TTGGGGTTCT TGCTCTGTGT CTTGTGGAAG AGGAAATCAA
    GCCCGCTATG TAAGTTGTCG TGATGCTCAT GATGGAATAG CAGATGAATC ATTTTGTGCC
    CACTTACCCC GACCAGCTGA AATAGCTGTC TGTTTTAGCC TTTGTGGAGA GTGGCAAACA
    GGGAATTGGT CACCTTGTTC AGCTTCCTGT GGCCGTGGAA AAATGACTCG CCAAGTTTTA
    TGCATCAACT ATCATCAGCC AGTTAATGAG AATTACTGTG ACCCCGAAGT CCGCCCTGTG
    ATAGAACAAG AATGTAATCT GGCAGCCTGC CCTCCCACGT ACAGCCACTT TCCGAGTTCC
    TCTGAGCAGC CAAGCCATTT TCCAGGCAGG AATTTTCCAT TAACTCACAA ACCAGAAGAT
    AATCAAAATC AGGGGATCCA TCCATCAATC AGAGAAAACC AGTGGAGAAC AGGACCATGG
    GGATCATGCT CTAGCAGTTG TGCTGGGGGT GTTCAACATC GAGTTGTGGT CTGCCAGGAT
    GAAAATGGAC AAAGTGCCAG TTACTGTGAT TCAGCGTCCA AACCCCCAGA GTCAAAGCAC
    TGTGATTCGG GACCTTGTCC ACAGTGGAAC TATGGAAGCT GGGGAGAATG TACACAAACA
    TGTGGAGGAG GAATAAAATC GAGATTTGTA ATATGTCAGT TTCCCAATGG CCAACTGTCA
    CAAGAGCAAA ACTGTGAAAT GTTGAACAAG CCACCTAGTG TGGTGCAGTG TCATATACGT
    GCTTGCCCTG ATGATGTATC ATGGCATCGG GGACCATGGA AATCGTGCTC AGCTTCCTGT
    GGTAAAGGTT TAAAATACCG TGAGGTGCTT TGCATTGACC AATTCCACGG GAAATTAGAA
    GAAAAATATT GCAGTCATCT ACAGAAACCT CGAACGCATA AAGCTTGTAG GTCTGTCCAA
    TGCCCTTCAT GGAAAGCCAA CAGATGGAAG GAGTGCTCTG TGACCTGCGG CTCCGGAGTT
    CAGCAACGGG ATGTGTACTG CAGACTGAGA GGAGTTGGTC GGGTGGCGGA GGAAAAGTGT
    GATCCATCCA CACGGCCTTA TTTTCAGAGA GAGTGTTGGC ATCGGAACTG CACACAGTAC
    CACTGGGTGG CAGGAGAATG GTTGGATTGC TCAATATCGT GTAAGAAAAG AGAGACATAT
    CGGCAAGTGA AGTGCGTGGA TGCACAAAAC ATTGAAGTGA GTGAGAGTTT CTGTGACCTC
    GCAACCAGAC CTCTCGCGAT CAAAAAGTGC AGAAACTCTC CCTGCAAGTA TATCGTGATA
    ACAGGAGACT CATCCCAGTG TGCAGGTAAC TGTGGATTTA GTTATCGACA AAGGATTACA
    TATTGCATTG GGGTCCAATC TATGGAGAAA CATAAGCTTC ATGAGCTGTG GCCTATAGGC
    TATCGAGTAT GCCCTGTGCT GCCTTCCCCT CAGGTTTACA AATGCAATTT TAAGACCTGT
    TTGCATATGG CCACTTGGAA AGTTGGAAAA TGGAGCAAGT GCTCTGTGAC TTGTGGAGTT
    GGGATGATGG AGAGAAGAGT GGAATGCATG GCCGATAATG GTCTGTCCAG TGATTTATGT
    TTAAAGCATT TAAAACCAGA TGCTCAAAAG AAATGTTATG TCAGTGACTG TAAAACATTT
    ACCAGCTGTA AAGAAATACA AATGAAAAAC AACATTACCA AGGATGGTGA CTATTACCTT
    AACATTAAAG GAAGAATAAC AAAGATCTAT TGTGCTGGCA TGCACTTGCA GAACCCTAAG
    GAATATATAT CATTGGTCAA AGGTGAAGAA GACAACTTTT CTGAAGTGTA TGGTTTTAGA
    CTACGAAATC CATATGAATG TCCCTTTAAT GGAAGCAGGA GGCAAGACTG TGAATGTAAA
    AATGACTATA TGGCTGCTGG ACACACTGTT TTTAGCAAAA TAAGAATTGA TCTTACTTCC
    ATGCAAATTA AAACTACAGA CCTTCTTTTT GCCCAGACAA TATTTGGAAA TGCAGTTCCA
    TTTGCCACAG CTGGAGATTG CTATAGTGCT GCCAGATGCC CACAGGGACA GTTCAGCATT
    AATTTGGCAG GAACTGGGAT GAAGATATCC AGTACAGCAA AGTGGCTGGC TCAGGGCAGT
    TATGCCTCTG TGATCATTCA CAGATCACAG GATGGAACCA AAGTCTATGG CAGATGTGGA
    GGATTCTGTG GAAAGTGTAT TCCTCACATG ACTACTGGTC TCCCAATTCA AGTAGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326515.1
    CDS309..5828
    Translation

    Target ORF information:

    RefSeq Version XM_015471029.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471029.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGCGGGTGG TCAAGTGGCT GACGGGGCTG CTCTACCAGC TCTCGCTCTT CATCACCAGG 
    TCTTGGGAAG TTCACTTCCA CCCCAGGCAG GAAGCCTTAG TGAGGACACT GGCCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAATGAG TTCGGCGAAG TATTCCCACA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCGGAGACT CTGGAGCCCA CACCGTTCAG AACCCACTAC
    CGAATCCGCG CCTACGGGCA GCTCTTCCAG CTGAACCTGA GCGCCGACGC GGCCTTCCTG
    GCTGCGGGAT ACACCGAGGT GCACTTAGGC ACTTCTGCGC ACCCGGCGGA GGAGCGCAGC
    GCGGCGCCCC CAGACCTACG CCACTGCTTC TACCGCGGCC AGGTCAATGC TCGCGAGGAT
    CACACAGCCG TCTTCAGCCT CTGTGGAGGC TTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGTG AACATGAAGA TGATCATAAC
    AAGCCACATC TTATATACAG ACAGGAATTA AAGAGGAACT ATTTTCTGCA GTCTCACAAG
    CCTTGTGATG TTTCAGAAAG TGAAATAAAG AAAACTACTT TACCCTTTCA TAACTACAGC
    GACGTGAATG AAAATCTCAA TATAAAGGAG GAAAGAGTTT TAGGATACAC TTCAAAAAAT
    ATATCTTTGG AAGATGAAAG AAGTCAATTG CATTCCAGGA AAAAACGTTT TTTATCATAT
    CCAAGATACG TGGAAGTTAT GGTTACAGCT GACTCTAAAA TGGTTCATCA TCACGGACAG
    AACTTGCAGC ACTATGTACT AACTCTGATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTTATAAA CATAGTAATT GTAAAATTAG TTATAATTCA TGATGAGCAG
    GAAGGACCAG TCATCAGTTT TAATGCAGCT ACCACATTAC GCAACTTCTG TTTATGGCAA
    CAAACTCAAA ATGTTCTTGA CGATGCCCAC CCATCCCACC ATGATACTGC TGTTCTTATC
    ACAAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTAGGTTT AGCAGAATTA
    GGTACCCTGT GCGATCCTTT ACGGAGCTGC TCCATTAGTG AAGAAAATGG ACTCAGTGCT
    GCCTTTACCA TAGCCCATGA GCTTGGTCAT GTATTTAATG TTCCACACGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAT TAAACATCAG TATCATGTCA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACCGGTCATG GGGAATGCCT CCTTGACAAA CCAAATGGAA GAATATATGA TCTATCTTCA
    CAGCTTCCTG GATTAATGTA TGATGTAAAC AAGCAATGTG AGCTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCCTATTT AAAACAATGC AGGCGTCTCT GGTGTACCAG TGCAGAAGGA
    GTTCACAAGG GCTGTCGCAC TCAACACATG CCACTGGCAG ATGGAACAAA CTGTGGTCCT
    GGGATGCATT GCCACCATGG GCTATGTGTA AATAAAGAAA TGGAAACACG TCCTGTAGAT
    GGCGATTGGG GACCATGGGG ACCTTACAGT TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA GCAGGCTCTG TAATCGCCCC GAGCCAAGAA ACGGAGGCAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAC ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTCCGTGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTCTCC
    CCTAACGTGA GGTGGCTTCC AAAGTACAGT GGGATTGCTG TAAAAGATCG TTGTAAACTC
    TATTGTCGGG TGGCTGGAAC CACTAGCTTC TACCAACTGA AGGACAGGGT TGCCGATGGC
    ACTCCTTGTG GAACTGAAAC AAATGACATC TGTGTTCAAG GCCTATGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAGT GTGGTGTGTG TGGTGGGGAT
    AACTCCTCCT GCAGAACAAT GGCAGGTGTC TTCAACAGTG CACATTATGG TTATAATGTC
    GTTGTAAAGA TTCCCCCAGG AGCAACAAAC ATTGATATTC TTCAGCACAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTGGCATTA TCGGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCCAAAAGG GAAATCAACG TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAACTC AATTGAAAGG ATTAATAGTA CTGATCGACT GGAAGAAGAA
    CTTGTTTTGC AGGTTTTGTG TGTGGGTAAT CTATACAACC CTGATGTACG TTATTCCTTC
    AATATCCCCG TGGAAGAGAA GAGTGATCTA TTCACATGGG ACCCATATGG ACCGTGGCAA
    GACTGTACAA AAATGTGTCA AGGTCTTCAT AGAAGAAAAA TTACTTGTGT ACGTAAGAGT
    GATCATATGG TCGTGTCTGA TCAAAGCTGT GACCACATAC CACTTCCATT GCTGGTGACT
    GAAAGGTGCA ATACAGACTG TGAACTAAGG TGGCACATCA TTGGCAAAAG TGAATGTTCA
    GCCCTTTGCG GTCAAGGATA CAAGTCACTG GACGTCCATT GTATGAAGTA TTCCATTCAT
    AACGGACAGA CTGTTCCAGT AGTTGACCAC TACTGCAGTG ACCAACTTAA GCCTCCAACC
    CGAGAACCCT GCCATGGTGA TTGTGTCTTA ACAAGGTGGC ATTATTTAGA ATGGTCTCAG
    TGCTCCAGAA GTTGTGGAGG AGGGGAGAGG TCTCGAGACT CTTACTGTAT AAATAACTTT
    GGCCACCGTC TTGCTGACAG AGAATGCCAG GAACTTCCCC GAGTGACAGT AGAGAATTGC
    AATGAATTTT CCTGTCCCAG TTGGGCCACT AGTGAGTGGA GTGAGTGTCT GGTTACCTGT
    GGAAAAGGAA CAAACCAGCG GCAGGTGTGG TGTCAACTGA ATGAAGATCA CTTGAGTGAC
    GGCTTCTGTG ACCCAAGCAC CAAGCCTGAG TCTCAGAGAC CCTGTGAGCT TCATGCGTGT
    ACTTCCTGGC AAGTAGGACC TTGGGGTTCT TGCTCTGTGT CTTGTGGAAG AGGAAATCAA
    GCCCGCTATG TAAGTTGTCG TGATGCTCAT GATGGAATAG CAGATGAATC ATTTTGTGCC
    CACTTACCCC GACCAGCTGA AATAGCTGTC TGTTTTAGCC TTTGTGGAGA GTGGCAAACA
    GGGAATTGGT CACCTTGTTC AGCTTCCTGT GGCCGTGGAA AAATGACTCG CCAAGTTTTA
    TGCATCAACT ATCATCAGCC AGTTAATGAG AATTACTGTG ACCCCGAAGT CCGCCCTGTG
    ATAGAACAAG AATGTAATCT GGCAGCCTGC CCTCCCACGT ACAGCCACTT TCCGAGTTCC
    TCTGAGCAGC CAAGCCATTT TCCAGGCAGG AATTTTCCAT TAACTCACAA ACCAGAAGAT
    AATCAAAATC AGGGGATCCA TCCATCAATC AGAGAAAACC AGTGGAGAAC AGGACCATGG
    GGATCATGCT CTAGCAGTTG TGCTGGGGGT GTTCAACATC GAGTTGTGGT CTGCCAGGAT
    GAAAATGGAC AAAGTGCCAG TTACTGTGAT TCAGCGTCCA AACCCCCAGA GTCAAAGCAC
    TGTGATTCGG GACCTTGTCC ACAGTGGAAC TATGGAAGCT GGGGAGAATG TACACAAACA
    TGTGGAGGAG GAATAAAATC GAGATTTGTA ATATGTCAGT TTCCCAATGG CCAACTGTCA
    CAAGAGCAAA ACTGTGAAAT GTTGAACAAG CCACCTAGTG TGGTGCAGTG TCATATACGT
    GCTTGCCCTG ATGATGTATC ATGGCATCGG GGACCATGGA AATCGTGCTC AGCTTCCTGT
    GGTAAAGGTT TAAAATACCG TGAGGTGCTT TGCATTGACC AATTCCACGG GAAATTAGAA
    GAAAAATATT GCAGTCATCT ACAGAAACCT CGAACGCATA AAGCTTGTAG GTCTGTCCAA
    TGCCCTTCAT GGAAAGCCAA CAGATGGAAG GAGTGCTCTG TGACCTGCGG CTCCGGAGTT
    CAGCAACGGG ATGTGTACTG CAGACTGAGA GGAGTTGGTC GGGTGGCGGA GGAAAAGTGT
    GATCCATCCA CACGGCCTTA TTTTCAGAGA GAGTGTTGGC ATCGGAACTG CACACAGTAC
    CACTGGGTGG CAGGAGAATG GTTGGATTGC TCAATATCGT GTAAGAAAAG AGAGACATAT
    CGGCAAGTGA AGTGCGTGGA TGCACAAAAC ATTGAAGTGA GTGAGAGTTT CTGTGACCTC
    GCAACCAGAC CTCTCGCGAT CAAAAAGTGC AGAAACTCTC CCTGCAAGTA TATCGTGATA
    ACAGGAGACT CATCCCAGTG TGCAGGTAAC TGTGGATTTA GTTATCGACA AAGGATTACA
    TATTGCATTG GGGTCCAATC TATGGAGAAA CATAAGCTTC ATGAGCTGTG GCCTATAGGC
    TATCGAGTAT GCCCTGTGCT GCCTTCCCCT CAGGTTTACA AATGCAATTT TAAGACCTGT
    TTGCATATGG CCACTTGGAA AGTTGGAAAA TGGAGCAAGT GCTCTGTGAC TTGTGGAGTT
    GGGATGATGG AGAGAAGAGT GGAATGCATG GCCGATAATG GTCTGTCCAG TGATTTATGT
    TTAAAGCATT TAAAACCAGA TGCTCAAAAG AAATGTTATG TCAGTGACTG TAAAACATTT
    ACCAGCTGTA AAGAAATACA AATGAAAAAC AACATTACCA AGGATGGTGA CTATTACCTT
    AACATTAAAG GAAGAATAAC AAAGATCTAT TGTGCTGGCA TGCACTTGCA GAACCCTAAG
    GAATATATAT CATTGGTCAA AGGTGAAGAA GACAACTTTT CTGAAGTGTA TGGTTTTAGA
    CTACGAAATC CATATGAATG TCCCTTTAAT GGAAGCAGGA GGCAAGACTG TGAATGTAAA
    AATGACTATA TGGCTGCTGG ACACACTGTT TTTAGCAAAA TAAGAATTGA TCTTACTTCC
    ATGCAAATTA AAACTACAGA CCTTCTTTTT GCCCAGACAA TATTTGGAAA TGCAGTTCCA
    TTTGCCACAG CTGGAGATTG CTATAGTGCT GCCAGATGCC CACAGGGACA GTTCAGCATT
    AATTTGGCAG GAACTGGGAT GAAGATATCC AGTACAGCAA AGTGGCTGGC TCAGGGCAGT
    TATGCCTCTG TGATCATTCA CAGATCACAG GATGGAACCA AAGTCTATGG CAGATGTGGA
    GGATTCTGTG GAAAGTGTAT TCCTCACATG ACTACTGGTC TCCCAATTCA AGTAGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53325
    Clone ID Related Accession (Same CDS sequence) XM_010805067.2
    Accession Version XM_010805067.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103922.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805067.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGCTTGG TCATGTGTCA GACAGGCTAC ACAGATCAAC AGACTAAGCA AAGAACTAGA 
    ACTTTGGAAC TCTTTTCTTT CATTTACTGT ATTGGATTAT ATGACAAGCA CTTCTGGCCT
    CTATCCCTTG TTATCCTATG CATGACCAGA TTTAATGTTC CACACGATGA TAGTTTTAAA
    TGTAAGGAAA CTGGAATTAA ACATCAGTAT CATGTCATGG CTCCAACTTT AAATTACCAC
    ACAAGTCCTT GGACCTGGTC AAAATGTAGT CAGAAATATA TCACTGAATT CCTAGATACC
    GGTCATGGGG AATGCCTCCT TGACAAACCA AATGGAAGAA TATATGATCT ATCTTCACAG
    CTTCCTGGAT TAATGTATGA TGTAAACAAG CAATGTGAGC TTATGTTTGG TCCTGGATCA
    CAAGTGTGTC CCTATTTAAA ACAATGCAGG CGTCTCTGGT GTACCAGTGC AGAAGGAGTT
    CACAAGGGCT GTCGCACTCA ACACATGCCA CTGGCAGATG GAACAAACTG TGGTCCTGGG
    ATGCATTGCC ACCATGGGCT ATGTGTAAAT AAAGAAATGG AAACACGTCC TGTAGATGGC
    GATTGGGGAC CATGGGGACC TTACAGTTCT TGTTCAAGAA CATGTGGAGG TGGAATCAAA
    AGCACAAGCA GGCTCTGTAA TCGCCCCGAG CCAAGAAACG GAGGCAAGTA CTGTGTGGGC
    CGCAGGATGA AATTTCGATC ATGTAACACT GATTCATGTC CAAAAGGCAA GCAAGACTTC
    CGTGAGAAGC AGTGCTCTGA TTTTGATGGT AAACATTTCA ACATCAATGG TCTCTCCCCT
    AACGTGAGGT GGCTTCCAAA GTACAGTGGG ATTGCTGTAA AAGATCGTTG TAAACTCTAT
    TGTCGGGTGG CTGGAACCAC TAGCTTCTAC CAACTGAAGG ACAGGGTTGC CGATGGCACT
    CCTTGTGGAA CTGAAACAAA TGACATCTGT GTTCAAGGCC TATGTCGGCA AGCTGGCTGT
    GATCATGTGT TAAACTCCAA GGCCAGGAGA GACAAGTGTG GTGTGTGTGG TGGGGATAAC
    TCCTCCTGCA GAACAATGGC AGGTGTCTTC AACAGTGCAC ATTATGGTTA TAATGTCGTT
    GTAAAGATTC CCCCAGGAGC AACAAACATT GATATTCTTC AGCACAGCTA TTCTGGAAAA
    CCAGAAGATG ACAATTACCT GGCATTATCG GACACTCAAG GGAATTTTCT TTTGAATGGG
    AATTTTGTTG TAAGTATGTC CAAAAGGGAA ATCAACGTAC AAGGAGCTAT TTTTGAATAC
    AGTGGTTCAA ACAACTCAAT TGAAAGGATT AATAGTACTG ATCGACTGGA AGAAGAACTT
    GTTTTGCAGG TTTTGTGTGT GGGTAATCTA TACAACCCTG ATGTACGTTA TTCCTTCAAT
    ATCCCCGTGG AAGAGAAGAG TGATCTATTC ACATGGGACC CATATGGACC GTGGCAAGAC
    TGTACAAAAA TGTGTCAAGG TCTTCATAGA AGAAAAATTA CTTGTGTACG TAAGAGTGAT
    CATATGGTCG TGTCTGATCA AAGCTGTGAC CACATACCAC TTCCATTGCT GGTGACTGAA
    AGGTGCAATA CAGACTGTGA ACTAAGGTGG CACATCATTG GCAAAAGTGA ATGTTCAGCC
    CTTTGCGGTC AAGGATACAA GTCACTGGAC GTCCATTGTA TGAAGTATTC CATTCATAAC
    GGACAGACTG TTCCAGTAGT TGACCACTAC TGCAGTGACC AACTTAAGCC TCCAACCCGA
    GAACCCTGCC ATGGTGATTG TGTCTTAACA AGGTGGCATT ATTTAGAATG GTCTCAGTGC
    TCCAGAAGTT GTGGAGGAGG GGAGAGGTCT CGAGACTCTT ACTGTATAAA TAACTTTGGC
    CACCGTCTTG CTGACAGAGA ATGCCAGGAA CTTCCCCGAG TGACAGTAGA GAATTGCAAT
    GAATTTTCCT GTCCCAGTTG GGCCACTAGT GAGTGGAGTG AGTGTCTGGT TACCTGTGGA
    AAAGGAACAA ACCAGCGGCA GGTGTGGTGT CAACTGAATG AAGATCACTT GAGTGACGGC
    TTCTGTGACC CAAGCACCAA GCCTGAGTCT CAGAGACCCT GTGAGCTTCA TGCGTGTACT
    TCCTGGCAAG TAGGACCTTG GGGTTCTTGC ACAGCCACGT GTGGACAGGG GTACCAGATG
    CGTGCTGTGA AATGTGTCAA CGAGCTATTT AGTGCAGTGC TAGATGACAG ACTGTGCCAT
    GGAGCTAGTC GCCCAAGTGA AAGGCAGGAT TGTATTGTTA TACCTTGTCC AGTTATCCCT
    AAAATTGGGG CCACTTCCTT GCCAGCTGTT CCCATGAGAA AGATAACACA ATGGCGATAT
    GGTTCTTGGA CCCCATGCTC TGTGTCTTGT GGAAGAGGAA ATCAAGCCCG CTATGTAAGT
    TGTCGTGATG CTCATGATGG AATAGCAGAT GAATCATTTT GTGCCCACTT ACCCCGACCA
    GCTGAAATAG CTGTCTGTTT TAGCCTTTGT GGAGAGTGGC AAACAGGGAA TTGGTCACCT
    TGTTCAGCTT CCTGTGGCCG TGGAAAAATG ACTCGCCAAG TTTTATGCAT CAACTATCAT
    CAGCCAGTTA ATGAGAATTA CTGTGACCCC GAAGTCCGCC CTGTGATAGA ACAAGAATGT
    AATCTGGCAG CCTGCCCTCC CACGTACAGC CACTTTCCGA GTTCCTCTGA GCAGCCAAGC
    CATTTTCCAG GCAGGAATTT TCCATTAACT CACAAACCAG AAGATAATCA AAATCAGGGG
    ATCCATCCAT CAATCAGAGA AAACCAGTGG AGAACAGGAC CATGGGGATC ATGCTCTAGC
    AGTTGTGCTG GGGGTGTTCA ACATCGAGTT GTGGTCTGCC AGGATGAAAA TGGACAAAGT
    GCCAGTTACT GTGATTCAGC GTCCAAACCC CCAGAGTCAA AGCACTGTGA TTCGGGACCT
    TGTCCACAGT GGAACTATGG AAGCTGGGGA GAATGTACAC AAACATGTGG AGGAGGAATA
    AAATCGAGAT TTGTAATATG TCAGTTTCCC AATGGCCAAC TGTCACAAGA GCAAAACTGT
    GAAATGTTGA ACAAGCCACC TAGTGTGGTG CAGTGTCATA TACGTGCTTG CCCTGATGAT
    GTATCATGGC ATCGGGGACC ATGGAAATCG TGCTCAGCTT CCTGTGGTAA AGGTTTAAAA
    TACCGTGAGG TGCTTTGCAT TGACCAATTC CACGGGAAAT TAGAAGAAAA ATATTGCAGT
    CATCTACAGA AACCTCGAAC GCATAAAGCT TGTAGGTCTG TCCAATGCCC TTCATGGAAA
    GCCAACAGAT GGAAGGAGTG CTCTGTGACC TGCGGCTCCG GAGTTCAGCA ACGGGATGTG
    TACTGCAGAC TGAGAGGAGT TGGTCGGGTG GCGGAGGAAA AGTGTGATCC ATCCACACGG
    CCTTATTTTC AGAGAGAGTG TTGGCATCGG AACTGCACAC AGTACCACTG GGTGGCAGGA
    GAATGGTTGG ATTGCTCAAT ATCGTGTAAG AAAAGAGAGA CATATCGGCA AGTGAAGTGC
    GTGGATGCAC AAAACATTGA AGTGAGTGAG AGTTTCTGTG ACCTCGCAAC CAGACCTCTC
    GCGATCAAAA AGTGCAGAAA CTCTCCCTGC AAGTATATCG TGATAACAGG AGACTCATCC
    CAGTGTGCAG GTAACTGTGG ATTTAGTTAT CGACAAAGGA TTACATATTG CATTGGGGTC
    CAATCTATGG AGAAACATAA GCTTCATGAG CTGTGGCCTA TAGGCTATCG AGTATGCCCT
    GTGCTGCCTT CCCCTCAGGT TTACAAATGC AATTTTAAGA CCTGTTTGCA TATGGCCACT
    TGGAAAGTTG GAAAATGGAG CAAGTGCTCT GTGACTTGTG GAGTTGGGAT GATGGAGAGA
    AGAGTGGAAT GCATGGCCGA TAATGGTCTG TCCAGTGATT TATGTTTAAA GCATTTAAAA
    CCAGATGCTC AAAAGAAATG TTATGTCAGT GACTGTAAAA CATTTACCAG CTGTAAAGAA
    ATACAAATGA AAAACAACAT TACCAAGGAT GGTGACTATT ACCTTAACAT TAAAGGAAGA
    ATAACAAAGA TCTATTGTGC TGGCATGCAC TTGCAGAACC CTAAGGAATA TATATCATTG
    GTCAAAGGTG AAGAAGACAA CTTTTCTGAA GTGTATGGTT TTAGACTACG AAATCCATAT
    GAATGTCCCT TTAATGGAAG CAGGAGGCAA GACTGTGAAT GTAAAAATGA CTATATGGCT
    GCTGGACACA CTGTTTTTAG CAAAATAAGA ATTGATCTTA CTTCCATGCA AATTAAAACT
    ACAGACCTTC TTTTTGCCCA GACAATATTT GGAAATGCAG TTCCATTTGC CACAGCTGGA
    GATTGCTATA GTGCTGCCAG ATGCCCACAG GGACAGTTCA GCATTAATTT GGCAGGAACT
    GGGATGAAGA TATCCAGTAC AGCAAAGTGG CTGGCTCAGG GCAGTTATGC CTCTGTGATC
    ATTCACAGAT CACAGGATGG AACCAAAGTC TATGGCAGAT GTGGAGGATT CTGTGGAAAG
    TGTATTCCTC ACATGACTAC TGGTCTCCCA ATTCAAGTAG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803369.1
    CDS74..4738
    Translation

    Target ORF information:

    RefSeq Version XM_010805067.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805067.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGCTTGG TCATGTGTCA GACAGGCTAC ACAGATCAAC AGACTAAGCA AAGAACTAGA 
    ACTTTGGAAC TCTTTTCTTT CATTTACTGT ATTGGATTAT ATGACAAGCA CTTCTGGCCT
    CTATCCCTTG TTATCCTATG CATGACCAGA TTTAATGTTC CACACGATGA TAGTTTTAAA
    TGTAAGGAAA CTGGAATTAA ACATCAGTAT CATGTCATGG CTCCAACTTT AAATTACCAC
    ACAAGTCCTT GGACCTGGTC AAAATGTAGT CAGAAATATA TCACTGAATT CCTAGATACC
    GGTCATGGGG AATGCCTCCT TGACAAACCA AATGGAAGAA TATATGATCT ATCTTCACAG
    CTTCCTGGAT TAATGTATGA TGTAAACAAG CAATGTGAGC TTATGTTTGG TCCTGGATCA
    CAAGTGTGTC CCTATTTAAA ACAATGCAGG CGTCTCTGGT GTACCAGTGC AGAAGGAGTT
    CACAAGGGCT GTCGCACTCA ACACATGCCA CTGGCAGATG GAACAAACTG TGGTCCTGGG
    ATGCATTGCC ACCATGGGCT ATGTGTAAAT AAAGAAATGG AAACACGTCC TGTAGATGGC
    GATTGGGGAC CATGGGGACC TTACAGTTCT TGTTCAAGAA CATGTGGAGG TGGAATCAAA
    AGCACAAGCA GGCTCTGTAA TCGCCCCGAG CCAAGAAACG GAGGCAAGTA CTGTGTGGGC
    CGCAGGATGA AATTTCGATC ATGTAACACT GATTCATGTC CAAAAGGCAA GCAAGACTTC
    CGTGAGAAGC AGTGCTCTGA TTTTGATGGT AAACATTTCA ACATCAATGG TCTCTCCCCT
    AACGTGAGGT GGCTTCCAAA GTACAGTGGG ATTGCTGTAA AAGATCGTTG TAAACTCTAT
    TGTCGGGTGG CTGGAACCAC TAGCTTCTAC CAACTGAAGG ACAGGGTTGC CGATGGCACT
    CCTTGTGGAA CTGAAACAAA TGACATCTGT GTTCAAGGCC TATGTCGGCA AGCTGGCTGT
    GATCATGTGT TAAACTCCAA GGCCAGGAGA GACAAGTGTG GTGTGTGTGG TGGGGATAAC
    TCCTCCTGCA GAACAATGGC AGGTGTCTTC AACAGTGCAC ATTATGGTTA TAATGTCGTT
    GTAAAGATTC CCCCAGGAGC AACAAACATT GATATTCTTC AGCACAGCTA TTCTGGAAAA
    CCAGAAGATG ACAATTACCT GGCATTATCG GACACTCAAG GGAATTTTCT TTTGAATGGG
    AATTTTGTTG TAAGTATGTC CAAAAGGGAA ATCAACGTAC AAGGAGCTAT TTTTGAATAC
    AGTGGTTCAA ACAACTCAAT TGAAAGGATT AATAGTACTG ATCGACTGGA AGAAGAACTT
    GTTTTGCAGG TTTTGTGTGT GGGTAATCTA TACAACCCTG ATGTACGTTA TTCCTTCAAT
    ATCCCCGTGG AAGAGAAGAG TGATCTATTC ACATGGGACC CATATGGACC GTGGCAAGAC
    TGTACAAAAA TGTGTCAAGG TCTTCATAGA AGAAAAATTA CTTGTGTACG TAAGAGTGAT
    CATATGGTCG TGTCTGATCA AAGCTGTGAC CACATACCAC TTCCATTGCT GGTGACTGAA
    AGGTGCAATA CAGACTGTGA ACTAAGGTGG CACATCATTG GCAAAAGTGA ATGTTCAGCC
    CTTTGCGGTC AAGGATACAA GTCACTGGAC GTCCATTGTA TGAAGTATTC CATTCATAAC
    GGACAGACTG TTCCAGTAGT TGACCACTAC TGCAGTGACC AACTTAAGCC TCCAACCCGA
    GAACCCTGCC ATGGTGATTG TGTCTTAACA AGGTGGCATT ATTTAGAATG GTCTCAGTGC
    TCCAGAAGTT GTGGAGGAGG GGAGAGGTCT CGAGACTCTT ACTGTATAAA TAACTTTGGC
    CACCGTCTTG CTGACAGAGA ATGCCAGGAA CTTCCCCGAG TGACAGTAGA GAATTGCAAT
    GAATTTTCCT GTCCCAGTTG GGCCACTAGT GAGTGGAGTG AGTGTCTGGT TACCTGTGGA
    AAAGGAACAA ACCAGCGGCA GGTGTGGTGT CAACTGAATG AAGATCACTT GAGTGACGGC
    TTCTGTGACC CAAGCACCAA GCCTGAGTCT CAGAGACCCT GTGAGCTTCA TGCGTGTACT
    TCCTGGCAAG TAGGACCTTG GGGTTCTTGC ACAGCCACGT GTGGACAGGG GTACCAGATG
    CGTGCTGTGA AATGTGTCAA CGAGCTATTT AGTGCAGTGC TAGATGACAG ACTGTGCCAT
    GGAGCTAGTC GCCCAAGTGA AAGGCAGGAT TGTATTGTTA TACCTTGTCC AGTTATCCCT
    AAAATTGGGG CCACTTCCTT GCCAGCTGTT CCCATGAGAA AGATAACACA ATGGCGATAT
    GGTTCTTGGA CCCCATGCTC TGTGTCTTGT GGAAGAGGAA ATCAAGCCCG CTATGTAAGT
    TGTCGTGATG CTCATGATGG AATAGCAGAT GAATCATTTT GTGCCCACTT ACCCCGACCA
    GCTGAAATAG CTGTCTGTTT TAGCCTTTGT GGAGAGTGGC AAACAGGGAA TTGGTCACCT
    TGTTCAGCTT CCTGTGGCCG TGGAAAAATG ACTCGCCAAG TTTTATGCAT CAACTATCAT
    CAGCCAGTTA ATGAGAATTA CTGTGACCCC GAAGTCCGCC CTGTGATAGA ACAAGAATGT
    AATCTGGCAG CCTGCCCTCC CACGTACAGC CACTTTCCGA GTTCCTCTGA GCAGCCAAGC
    CATTTTCCAG GCAGGAATTT TCCATTAACT CACAAACCAG AAGATAATCA AAATCAGGGG
    ATCCATCCAT CAATCAGAGA AAACCAGTGG AGAACAGGAC CATGGGGATC ATGCTCTAGC
    AGTTGTGCTG GGGGTGTTCA ACATCGAGTT GTGGTCTGCC AGGATGAAAA TGGACAAAGT
    GCCAGTTACT GTGATTCAGC GTCCAAACCC CCAGAGTCAA AGCACTGTGA TTCGGGACCT
    TGTCCACAGT GGAACTATGG AAGCTGGGGA GAATGTACAC AAACATGTGG AGGAGGAATA
    AAATCGAGAT TTGTAATATG TCAGTTTCCC AATGGCCAAC TGTCACAAGA GCAAAACTGT
    GAAATGTTGA ACAAGCCACC TAGTGTGGTG CAGTGTCATA TACGTGCTTG CCCTGATGAT
    GTATCATGGC ATCGGGGACC ATGGAAATCG TGCTCAGCTT CCTGTGGTAA AGGTTTAAAA
    TACCGTGAGG TGCTTTGCAT TGACCAATTC CACGGGAAAT TAGAAGAAAA ATATTGCAGT
    CATCTACAGA AACCTCGAAC GCATAAAGCT TGTAGGTCTG TCCAATGCCC TTCATGGAAA
    GCCAACAGAT GGAAGGAGTG CTCTGTGACC TGCGGCTCCG GAGTTCAGCA ACGGGATGTG
    TACTGCAGAC TGAGAGGAGT TGGTCGGGTG GCGGAGGAAA AGTGTGATCC ATCCACACGG
    CCTTATTTTC AGAGAGAGTG TTGGCATCGG AACTGCACAC AGTACCACTG GGTGGCAGGA
    GAATGGTTGG ATTGCTCAAT ATCGTGTAAG AAAAGAGAGA CATATCGGCA AGTGAAGTGC
    GTGGATGCAC AAAACATTGA AGTGAGTGAG AGTTTCTGTG ACCTCGCAAC CAGACCTCTC
    GCGATCAAAA AGTGCAGAAA CTCTCCCTGC AAGTATATCG TGATAACAGG AGACTCATCC
    CAGTGTGCAG GTAACTGTGG ATTTAGTTAT CGACAAAGGA TTACATATTG CATTGGGGTC
    CAATCTATGG AGAAACATAA GCTTCATGAG CTGTGGCCTA TAGGCTATCG AGTATGCCCT
    GTGCTGCCTT CCCCTCAGGT TTACAAATGC AATTTTAAGA CCTGTTTGCA TATGGCCACT
    TGGAAAGTTG GAAAATGGAG CAAGTGCTCT GTGACTTGTG GAGTTGGGAT GATGGAGAGA
    AGAGTGGAAT GCATGGCCGA TAATGGTCTG TCCAGTGATT TATGTTTAAA GCATTTAAAA
    CCAGATGCTC AAAAGAAATG TTATGTCAGT GACTGTAAAA CATTTACCAG CTGTAAAGAA
    ATACAAATGA AAAACAACAT TACCAAGGAT GGTGACTATT ACCTTAACAT TAAAGGAAGA
    ATAACAAAGA TCTATTGTGC TGGCATGCAC TTGCAGAACC CTAAGGAATA TATATCATTG
    GTCAAAGGTG AAGAAGACAA CTTTTCTGAA GTGTATGGTT TTAGACTACG AAATCCATAT
    GAATGTCCCT TTAATGGAAG CAGGAGGCAA GACTGTGAAT GTAAAAATGA CTATATGGCT
    GCTGGACACA CTGTTTTTAG CAAAATAAGA ATTGATCTTA CTTCCATGCA AATTAAAACT
    ACAGACCTTC TTTTTGCCCA GACAATATTT GGAAATGCAG TTCCATTTGC CACAGCTGGA
    GATTGCTATA GTGCTGCCAG ATGCCCACAG GGACAGTTCA GCATTAATTT GGCAGGAACT
    GGGATGAAGA TATCCAGTAC AGCAAAGTGG CTGGCTCAGG GCAGTTATGC CTCTGTGATC
    ATTCACAGAT CACAGGATGG AACCAAAGTC TATGGCAGAT GTGGAGGATT CTGTGGAAAG
    TGTATTCCTC ACATGACTAC TGGTCTCCCA ATTCAAGTAG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.