NOTCH4 cDNA ORF clone, Bos taurus(cattle)

The following NOTCH4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOTCH4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa239105 NM_001206948.2
Latest version!
Bos taurus notch receptor 4 (NOTCH4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa229526 XM_024983664.1
Latest version!
Bos taurus notch 4 (NOTCH4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa32161 XM_010818347.2
Latest version!
Bos taurus notch 4 (NOTCH4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa38379 NM_001206948.1
Latest version!
Bos taurus notch 4 (NOTCH4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa32160 XM_005223669.3
Latest version!
Bos taurus notch 4 (NOTCH4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa32160 XM_010818346.2
Latest version!
Bos taurus notch 4 (NOTCH4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa239105
    Clone ID Related Accession (Same CDS sequence) NM_001206948.2
    Accession Version NM_001206948.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-08
    Organism Bos taurus(cattle)
    Product neurogenic locus notch homolog protein 4 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000023.1. On May 30, 2018 this sequence version replaced NM_001206948.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCAGCCCC CTTCACTGCT GCTGCCACTG CTGCTGCTGC TGTGTCAGCC AGTCGTCAGG 
    ACCAGAGGGC TGCAGTGCGG GAGTTTCCCA GAACCCTGTG CCAACGGAGG CACCTGCCTG
    AGCCTATCCC AGGGGCAAGG GACCTGCCAG TGTGCCCCTG GCTTCCTGGG TGAGACATGC
    CAGTTTCCTG ACCCCTGCCA GGATGCCCAG CCCTGCCAGA ACGGAGGCAG CTGCCACACC
    TTTCTTCCCA CCCTTCCCGG CTCCCCTGGC ACCCCCTCTC CCATGGCCCC CAGCTTCTTC
    TGCACCTGCC CCTCTGGCTT CACTGGCGAT AGGTGCCAGG CCCAGATCAG GGACCCCTGT
    TCCTCCTTCT GTTCCAAAAT GGGTCGCTGC CACCTCCAGG ACTCGGGCCG CCCACGATGC
    TCCTGCATGC CAGGATGGAC GGGTGAGCAC TGCCAGCTTC GAGACTTCTG CTCAGCCAAC
    CCCTGCGTCA ATGGAGGAGT GTGTCTGGCC ACCTACCCCC AGATCCAGTG CCGCTGCCCA
    CCCGGCTTCG AGGGCCACGC CTGCGAACAC GACGTCAACG AGTGTTACCT GGACCCGGGA
    CCCTGCCCCA AGGGCACCAC CTGCCATAAC ACCCTGGGAT CCTTCCAGTG TCTCTGCCCT
    GCTGGGCGGG AGGGTCCACG CTGTGGACTC CGGCCGGGAC CCTGCACCCC CAGGGGCTGT
    CTCAATGGGG GCACCTGTCA ACTGGTTCCA GGGAGAGACT CCACTTTCCA CCTGTGCCTC
    TGTCCCCCAG GCTTCACAGG CCCAAGCTGT GAGGTGAATC CAGATGACTG TGCTGGGCAC
    CAGTGTCAGA ACGGGGGCAC CTGCCAGGAT GGGCTGGGCA CCTACACCTG CCTCTGCCCA
    GAGGCCTGGA CAGGCTGGGA TTGCTCTGAA GATGTGGACG AATGTGAGGT TCAGGGTCCC
    CCTCGCTGCA GAAATGGGGG TACCTGCCAG AACTCAGCTG GAGACTTCTA CTGTGTGTGT
    GTGAGTGGCT GGGGAGGCGC CGGCTGCGAA GAGAACCTGG ACGACTGTGT GGCCGCCACC
    TGTGCCCCCG GATCCACCTG CATTGACCGC GTGGGCTCCT TCTCCTGCCT CTGCCCGCCT
    GGGCGCACAG GCCTCCTGTG CCACATGGAG GACATGTGTC TGAGTCAGCC ATGCCATGAG
    GAAGCCCAGT GCAGCACCAA CCCCCTGAGT GGCTCCACAC TCTGCGTATG TCAGCCAGGC
    TACACAGGGC CCACCTGCCA CCAGGACCTG GACGAGTGTC AGATGGCTCA GCAAGGCCCC
    AGTCCCTGTG AACACGGCGG CTCCTGCCTC AACACGCCCG GCTCCTTCGA GTGCCTCTGT
    CCGCCTGGCT ACACGGGTTC CCGCTGCGAG GCCGATCACA ACGAGTGCCT GTCGCAGCCC
    TGCCACAGGG GCGGCACCTG CCTGGACCTG CTCGCCACCT TCCAGTGCCT CTGCCCACCA
    GGCTTAGAAG GGCAGCTCTG TGAGGTGGAG ATAGACGAGT GCGCCTCGGC CCCCTGCTTG
    AACCAGGCTG ACTGCCACGA CCTGCTCAAC GGCTTCCAGT GCATCTGCCA GCCAGGTTTC
    ACTGGCCCCC GGTGTGAGGA GGACATCAAC GAATGTCAAA GCTCTCCCTG TGCCAATGGC
    GGGGAGTGCC AGGACCAGCC TGGATCCTTC CATTGCAAGT GTCCCCCAGG CTTTGAAGGC
    CCACGCTGTC AGGAGGAGGT GGACGAGTGT CTGAGTGGCC CATGCCCCAC CGGAGCCAGC
    TGCCTTGACC TCCCAGGAGC TTTCCTTTGC GTCTGCCCCT CTGGCTTCAC AGGCCATCTC
    TGTGAAATTC CCCTGTGTGC CCCCAACCTG TGCCAGCCCA AACAAAAATG TCAGGATCAG
    GAGGACAATG CCCACTGCCT CTGCCCTGAT GGAAACCCAG GCTGTGTCCC CACAGAAGAC
    AACTGCACCT GCCACCATGG GCACTGCCAG AGATCTTCAT GTGTGTGTGA TGGGGGCTGG
    ACAGGACCAG AGTGTGACAC AGATCTGGGG GGCTGTGTCT CCACCCCCTG TGCCCACGGA
    GGGACCTGCC ACCCCCAGCC CTTTGGCTAC AACTGCACCT GCCCCACAGG TTACACAGGG
    CCCACTTGCA GTGAGGAGGT GACAGCCTGT CACTCAGGGC CCTGTCTCAA TGGTGGTTCC
    TGCAGCCCCA GCCCCGGGGG CTACACCTGC ACCTGCCCTC TGAGCCACAC GGGGCTCCGC
    TGCCAGACCA GCATTGACCA TTGTGCCTCT GCACTGTGCC TCAATGGGGG CACCTGTGTG
    AACAAGCCGG GCACCTTCTC CTGCCTCTGC ACCCCAGGCT TCCAGGGCCC ACGCTGCGAG
    GGAAGGACCC GTCCCAGCTG CGCAGACAGC CCCTGTAGGA ACATGGCAAC CTGCCAAGAC
    AGCCCTCAGG GTCCCCGCTG CCTCTGTCCC CCTGGCTACA CAGGAGGCAG CTGTCAGACG
    CTGATGGACT TATGCGCCCA GAAGCCCTGC CCACAAAATT CCCACTGCCT CCAGACTGGG
    CCCTCCTTCC AGTGCCTGTG CCTCCAGGGA TGGACTGGGC CTCTCTGCAA CCTGCCGCTG
    TCCTCCTGCC AGAAGGTTGC TCTGAGCCAA GGCACAGAAG TCTCTTCCCT GTGCCAGAAC
    GGAGGCGTCT GCATTGACAG CGGCCCCTCC CATTTCTGCC ACTGTCCCCC TGGATTCCAA
    GGCAGCATAT GCCAGGACCA GGTGAACCCC TGTGAGTCCA GGCCCTGCCA GCATGGGGCC
    ACCTGCGTGG CCCAACCCAA CGGTTATCTC TGCCAGTGTG CACCCGGCTA CAGTGGACAG
    AACTGCTCAG AGGAACCGGA CGCATGTCAA TCCCAGCCCT GTCACAACCA GGGGACCTGT
    ATCTCCAAAC CCGGAGGCTT CCACTGTGCC TGCCCGCCAG GCTTTGTGGG GCTGCGCTGT
    GAGGGGGACG TGGATGAGTG TCTGGACCGG CCCTGTCACC CCACAGGCAC TGCAGCCTGC
    CACTCTCTGG CCAATGCCTT CTACTGCCAG TGTCTGCCTG GACACACAGG CCAGTGGTGT
    GAAGTGGAGC TAGACCCCTG CCAGAGCCAG CCCTGTGCCC ACAGCGGGTC ATGTGAGGCC
    ACAGCAGGGC CACCCCCAGG ATTCACCTGC CACTGTCCCC AGGGTTTTGA AGGCCCCACC
    TGCAGCCACA GAGCCCCCTC CTGTGGTCTC CATCACTGCC ACCATGGTGG CCTGTGTCTG
    CCCTCCCCCA AGCCTGGACT CCCACCCCGC TGCGCCTGCC TCAATGGTTA TGGGGGTCCG
    GATTGCCTGA CCCCACCAGC TCCTTCAGGC TGTGGCCCTC CTTCTCCATG CCTACACAAT
    GGCAGCTGCT CAGAGACCCC CGGGCTGGGG GGCCCAGGCT TTCGCTGTTC CTGCCCTGCC
    AGCTCTCCAG GGCCCCGGTG TCAGAGGCCC GGAGTCAAGG GATGCGAGGG CAGAAGTGGA
    GATGGGGCCT GTGATGCTGG CTGCAGTGGC CCAGGAGGAA ACTGGGATGG GGGGGACTGC
    TCCCTGGGGG TCCCAGACCC CTGGAAGGGC TGCCCCTCCC ACTCCCGGTG CTGGCTTCTT
    TTCCGGGACG GGCAGTGCCA CCCACAGTGT GACTCTGAAG AGTGTTTGTT TGATGGCTAC
    GACTGTGAGA CTCCTCCAGC CTGCATTCCA GCCTATGACC AGTACTGCCG CGATCACTTC
    CACAATGGAC ACTGCGAGAA GGGCTGCAAC ACGGCAGAAT GTGGCTGGGA CGGGGGAGAC
    TGCAGGCCGG AAGATGGAAA TTCAGAGTGG GGGCCCTCCC TGGCCTTGTT GGTGGTGCTG
    AGCCCCTCAA CCCTGGACCA GCAGCTGCTC GCCCTGGCCC GGGTGCTGTC CCTGACTCTG
    AGGGTAGGGC TCTGGGTGAG GAAGGACAGT GAAGGCAAGG ACATGGTGTA CCCCTATCCT
    GGAGCCCAAG CAGAAGAGGA GCTAGGAGGA ACCCCAGACC CCTCTCAGCA GGAGAGAGCA
    GCCCCCCAAA CAGAGCCTAC AGGCAAGGAG ACAGATTCCC TGAGCACTGG GTTTGTGGTG
    GTGATGGGTG TGGACTTGTC CCACTGTGGC CCCGAGCGCC CTGCATCCCG CTGCCCCTGG
    GACCCTGGGC TCCTACTCCG CTTCCTTGCT GCAATGGCTG CAGTGGGGGC CCTGGAACCC
    CTACTGCCAG GACCCCTGCT GGCTGCCCAC CCTCGTGCAG GCACCAAGCC CTCCGCCAAC
    CAGCTTCCCT GGCCTGTACT GTGCTCCCCA GTGGTCGGGG TCCTTCTCCT AGCCCTCGGG
    GCTCTTCTCG TCCTCCAGCT CATCCGTCGC AAGCGCCGAG AGCATGGGGC CCTCTGGCTG
    CCCCCTGGGT TCACTCGCAG GCCCCGGACT CAGCCAGTTT CCCGCCGACG TCGACCCCCG
    CTGGGCGAGG ACAGCATCGG CCTCAAGGCA CTGAAAGCAG AGGCAGACAT GGATGAGGAT
    GGTGTTGTGA TGTGCTCAGT CCCCAAGGAA GGAGAGGAGG CTGAAGAAAT GGCCTCACCC
    CCCAAGTGCC AGCTCTGGTC TCTGAGCAGT GACTGCCAAG AGCTCCCCCA GGCAGCCATG
    CTGACTCCTC CCCAGGATTC TGAGATGGAT GTCCCTGACG TGGACACCCA GGGACCTGAT
    GGGGTGACAC CCCTGATGTC AGCAGTCTGC TGTGGGGGCG TGGAGTCCAG GACCTACCAG
    GAGACATGGC TGGGAAGTCC TGAGCCCTGG GAACCTCTGC TGGAGGGAGG GGCCTGTCCC
    CAGGTTCACA CTGTGGGCAC TGGGGAGACA CCCCTGCACC TGGCTGCTCG ATTCTCCCGG
    CCAACCGCTG CCCGCCGCCT CCTCGAGGCT GGAGCAAACC CCAACCAGCC AGACCGGGCA
    GGGCGCACCC CGCTTCACAC GGCTGTGGCT GCTGATGCTA GGGAGGTTTG CCAGCTCTTA
    CTCCGCAGCA GACAGACTGC AGTGGATGCA CGGACAGAGG ATGGGACCAC AGCCCTGATG
    CTGGCCGCCA GGCTGGCTGT CGAAGACCTG GTTGAAGAAC TGATTGCAGC CCAAGCAGAT
    GTGGGGGCCA GAGACAAATG GGGAAAAACA GCGCTGCACT GGGCCGCCGC CGTGAACAAC
    GCCCGGGCCG CCCGCTCCCT TCTCCAGGCG GGAGCCGATA AAGACGCACA GGATGGCAGG
    GAGCAGACGC CGCTGTTCCT GGCGGCCCGA GAAGGGGCGA TGGAGGTAGC TCAGCTGCTG
    CTGGGGCTGG GAGCCGCCCA AGGACTACGG GACCAGGCCG GCTTAACACC CGGAGACGTC
    GCCCGTCAGC GCAACCACTG GGATCTGCTG ACGCTGCTGG AAGGGGCGGG GCCACCGGAG
    GCGCGTCACA AACCCACGCC GGGCCGCGGC GCCGGCACCT TCCAGCGCGC GCGGACCGCG
    TCCGGAAGCG TGCCCCCGCG TGGGGGCGGG GCTGTGCCGC GCTGCCGGAC GCTGTCAGCC
    GGAGCCCGTC CGCAAGCCCG GACTTTGTCC GTAGACTTGG CCGCGCACGG GGGCGGGGCC
    TACTCGCACT GCCGGAGCCT ATCGAAAGGA ACAGGAGAAG GCCCGCCCCT CCGCGGCCGT
    AGGTTCTCAG CAGGCATGCG CGGGCCTCGG CCCAACCCTG CAATGGTGCG AGGAAGACCC
    GGGGTCTCGG CCGGGAGCGG GGGCGTGGCT GCAACTGTTG ACTGGCCCTG CGACTGGGTG
    CCCCTGGGAG CCTGCGGCCC CGCTTCCAAC ACGCCAATCC CGCCTCCTTG CCTAACACCG
    TCCCCTGAGC GGGGATCCCC TCAAGTTGCC TGGGGTCCCC CAGCCCACCA AGTAATACCC
    TTAAATGCAG GAGGCGAGGG TCAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193877.2
    CDS1..5970
    Translation

    Target ORF information:

    RefSeq Version NM_001206948.2
    Organism Bos taurus(cattle)
    Definition Bos taurus notch receptor 4 (NOTCH4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206948.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCAGCCCC CTTCACTGCT GCTGCCACTG CTGCTGCTGC TGTGTCAGCC AGTCGTCAGG 
    ACCAGAGGGC TGCAGTGCGG GAGTTTCCCA GAACCCTGTG CCAACGGAGG CACCTGCCTG
    AGCCTATCCC AGGGGCAAGG GACCTGCCAG TGTGCCCCTG GCTTCCTGGG TGAGACATGC
    CAGTTTCCTG ACCCCTGCCA GGATGCCCAG CCCTGCCAGA ACGGAGGCAG CTGCCACACC
    TTTCTTCCCA CCCTTCCCGG CTCCCCTGGC ACCCCCTCTC CCATGGCCCC CAGCTTCTTC
    TGCACCTGCC CCTCTGGCTT CACTGGCGAT AGGTGCCAGG CCCAGATCAG GGACCCCTGT
    TCCTCCTTCT GTTCCAAAAT GGGTCGCTGC CACCTCCAGG ACTCGGGCCG CCCACGATGC
    TCCTGCATGC CAGGATGGAC GGGTGAGCAC TGCCAGCTTC GAGACTTCTG CTCAGCCAAC
    CCCTGCGTCA ATGGAGGAGT GTGTCTGGCC ACCTACCCCC AGATCCAGTG CCGCTGCCCA
    CCCGGCTTCG AGGGCCACGC CTGCGAACAC GACGTCAACG AGTGTTACCT GGACCCGGGA
    CCCTGCCCCA AGGGCACCAC CTGCCATAAC ACCCTGGGAT CCTTCCAGTG TCTCTGCCCT
    GCTGGGCGGG AGGGTCCACG CTGTGGACTC CGGCCGGGAC CCTGCACCCC CAGGGGCTGT
    CTCAATGGGG GCACCTGTCA ACTGGTTCCA GGGAGAGACT CCACTTTCCA CCTGTGCCTC
    TGTCCCCCAG GCTTCACAGG CCCAAGCTGT GAGGTGAATC CAGATGACTG TGCTGGGCAC
    CAGTGTCAGA ACGGGGGCAC CTGCCAGGAT GGGCTGGGCA CCTACACCTG CCTCTGCCCA
    GAGGCCTGGA CAGGCTGGGA TTGCTCTGAA GATGTGGACG AATGTGAGGT TCAGGGTCCC
    CCTCGCTGCA GAAATGGGGG TACCTGCCAG AACTCAGCTG GAGACTTCTA CTGTGTGTGT
    GTGAGTGGCT GGGGAGGCGC CGGCTGCGAA GAGAACCTGG ACGACTGTGT GGCCGCCACC
    TGTGCCCCCG GATCCACCTG CATTGACCGC GTGGGCTCCT TCTCCTGCCT CTGCCCGCCT
    GGGCGCACAG GCCTCCTGTG CCACATGGAG GACATGTGTC TGAGTCAGCC ATGCCATGAG
    GAAGCCCAGT GCAGCACCAA CCCCCTGAGT GGCTCCACAC TCTGCGTATG TCAGCCAGGC
    TACACAGGGC CCACCTGCCA CCAGGACCTG GACGAGTGTC AGATGGCTCA GCAAGGCCCC
    AGTCCCTGTG AACACGGCGG CTCCTGCCTC AACACGCCCG GCTCCTTCGA GTGCCTCTGT
    CCGCCTGGCT ACACGGGTTC CCGCTGCGAG GCCGATCACA ACGAGTGCCT GTCGCAGCCC
    TGCCACAGGG GCGGCACCTG CCTGGACCTG CTCGCCACCT TCCAGTGCCT CTGCCCACCA
    GGCTTAGAAG GGCAGCTCTG TGAGGTGGAG ATAGACGAGT GCGCCTCGGC CCCCTGCTTG
    AACCAGGCTG ACTGCCACGA CCTGCTCAAC GGCTTCCAGT GCATCTGCCA GCCAGGTTTC
    ACTGGCCCCC GGTGTGAGGA GGACATCAAC GAATGTCAAA GCTCTCCCTG TGCCAATGGC
    GGGGAGTGCC AGGACCAGCC TGGATCCTTC CATTGCAAGT GTCCCCCAGG CTTTGAAGGC
    CCACGCTGTC AGGAGGAGGT GGACGAGTGT CTGAGTGGCC CATGCCCCAC CGGAGCCAGC
    TGCCTTGACC TCCCAGGAGC TTTCCTTTGC GTCTGCCCCT CTGGCTTCAC AGGCCATCTC
    TGTGAAATTC CCCTGTGTGC CCCCAACCTG TGCCAGCCCA AACAAAAATG TCAGGATCAG
    GAGGACAATG CCCACTGCCT CTGCCCTGAT GGAAACCCAG GCTGTGTCCC CACAGAAGAC
    AACTGCACCT GCCACCATGG GCACTGCCAG AGATCTTCAT GTGTGTGTGA TGGGGGCTGG
    ACAGGACCAG AGTGTGACAC AGATCTGGGG GGCTGTGTCT CCACCCCCTG TGCCCACGGA
    GGGACCTGCC ACCCCCAGCC CTTTGGCTAC AACTGCACCT GCCCCACAGG TTACACAGGG
    CCCACTTGCA GTGAGGAGGT GACAGCCTGT CACTCAGGGC CCTGTCTCAA TGGTGGTTCC
    TGCAGCCCCA GCCCCGGGGG CTACACCTGC ACCTGCCCTC TGAGCCACAC GGGGCTCCGC
    TGCCAGACCA GCATTGACCA TTGTGCCTCT GCACTGTGCC TCAATGGGGG CACCTGTGTG
    AACAAGCCGG GCACCTTCTC CTGCCTCTGC ACCCCAGGCT TCCAGGGCCC ACGCTGCGAG
    GGAAGGACCC GTCCCAGCTG CGCAGACAGC CCCTGTAGGA ACATGGCAAC CTGCCAAGAC
    AGCCCTCAGG GTCCCCGCTG CCTCTGTCCC CCTGGCTACA CAGGAGGCAG CTGTCAGACG
    CTGATGGACT TATGCGCCCA GAAGCCCTGC CCACAAAATT CCCACTGCCT CCAGACTGGG
    CCCTCCTTCC AGTGCCTGTG CCTCCAGGGA TGGACTGGGC CTCTCTGCAA CCTGCCGCTG
    TCCTCCTGCC AGAAGGTTGC TCTGAGCCAA GGCACAGAAG TCTCTTCCCT GTGCCAGAAC
    GGAGGCGTCT GCATTGACAG CGGCCCCTCC CATTTCTGCC ACTGTCCCCC TGGATTCCAA
    GGCAGCATAT GCCAGGACCA GGTGAACCCC TGTGAGTCCA GGCCCTGCCA GCATGGGGCC
    ACCTGCGTGG CCCAACCCAA CGGTTATCTC TGCCAGTGTG CACCCGGCTA CAGTGGACAG
    AACTGCTCAG AGGAACCGGA CGCATGTCAA TCCCAGCCCT GTCACAACCA GGGGACCTGT
    ATCTCCAAAC CCGGAGGCTT CCACTGTGCC TGCCCGCCAG GCTTTGTGGG GCTGCGCTGT
    GAGGGGGACG TGGATGAGTG TCTGGACCGG CCCTGTCACC CCACAGGCAC TGCAGCCTGC
    CACTCTCTGG CCAATGCCTT CTACTGCCAG TGTCTGCCTG GACACACAGG CCAGTGGTGT
    GAAGTGGAGC TAGACCCCTG CCAGAGCCAG CCCTGTGCCC ACAGCGGGTC ATGTGAGGCC
    ACAGCAGGGC CACCCCCAGG ATTCACCTGC CACTGTCCCC AGGGTTTTGA AGGCCCCACC
    TGCAGCCACA GAGCCCCCTC CTGTGGTCTC CATCACTGCC ACCATGGTGG CCTGTGTCTG
    CCCTCCCCCA AGCCTGGACT CCCACCCCGC TGCGCCTGCC TCAATGGTTA TGGGGGTCCG
    GATTGCCTGA CCCCACCAGC TCCTTCAGGC TGTGGCCCTC CTTCTCCATG CCTACACAAT
    GGCAGCTGCT CAGAGACCCC CGGGCTGGGG GGCCCAGGCT TTCGCTGTTC CTGCCCTGCC
    AGCTCTCCAG GGCCCCGGTG TCAGAGGCCC GGAGTCAAGG GATGCGAGGG CAGAAGTGGA
    GATGGGGCCT GTGATGCTGG CTGCAGTGGC CCAGGAGGAA ACTGGGATGG GGGGGACTGC
    TCCCTGGGGG TCCCAGACCC CTGGAAGGGC TGCCCCTCCC ACTCCCGGTG CTGGCTTCTT
    TTCCGGGACG GGCAGTGCCA CCCACAGTGT GACTCTGAAG AGTGTTTGTT TGATGGCTAC
    GACTGTGAGA CTCCTCCAGC CTGCATTCCA GCCTATGACC AGTACTGCCG CGATCACTTC
    CACAATGGAC ACTGCGAGAA GGGCTGCAAC ACGGCAGAAT GTGGCTGGGA CGGGGGAGAC
    TGCAGGCCGG AAGATGGAAA TTCAGAGTGG GGGCCCTCCC TGGCCTTGTT GGTGGTGCTG
    AGCCCCTCAA CCCTGGACCA GCAGCTGCTC GCCCTGGCCC GGGTGCTGTC CCTGACTCTG
    AGGGTAGGGC TCTGGGTGAG GAAGGACAGT GAAGGCAAGG ACATGGTGTA CCCCTATCCT
    GGAGCCCAAG CAGAAGAGGA GCTAGGAGGA ACCCCAGACC CCTCTCAGCA GGAGAGAGCA
    GCCCCCCAAA CAGAGCCTAC AGGCAAGGAG ACAGATTCCC TGAGCACTGG GTTTGTGGTG
    GTGATGGGTG TGGACTTGTC CCACTGTGGC CCCGAGCGCC CTGCATCCCG CTGCCCCTGG
    GACCCTGGGC TCCTACTCCG CTTCCTTGCT GCAATGGCTG CAGTGGGGGC CCTGGAACCC
    CTACTGCCAG GACCCCTGCT GGCTGCCCAC CCTCGTGCAG GCACCAAGCC CTCCGCCAAC
    CAGCTTCCCT GGCCTGTACT GTGCTCCCCA GTGGTCGGGG TCCTTCTCCT AGCCCTCGGG
    GCTCTTCTCG TCCTCCAGCT CATCCGTCGC AAGCGCCGAG AGCATGGGGC CCTCTGGCTG
    CCCCCTGGGT TCACTCGCAG GCCCCGGACT CAGCCAGTTT CCCGCCGACG TCGACCCCCG
    CTGGGCGAGG ACAGCATCGG CCTCAAGGCA CTGAAAGCAG AGGCAGACAT GGATGAGGAT
    GGTGTTGTGA TGTGCTCAGT CCCCAAGGAA GGAGAGGAGG CTGAAGAAAT GGCCTCACCC
    CCCAAGTGCC AGCTCTGGTC TCTGAGCAGT GACTGCCAAG AGCTCCCCCA GGCAGCCATG
    CTGACTCCTC CCCAGGATTC TGAGATGGAT GTCCCTGACG TGGACACCCA GGGACCTGAT
    GGGGTGACAC CCCTGATGTC AGCAGTCTGC TGTGGGGGCG TGGAGTCCAG GACCTACCAG
    GAGACATGGC TGGGAAGTCC TGAGCCCTGG GAACCTCTGC TGGAGGGAGG GGCCTGTCCC
    CAGGTTCACA CTGTGGGCAC TGGGGAGACA CCCCTGCACC TGGCTGCTCG ATTCTCCCGG
    CCAACCGCTG CCCGCCGCCT CCTCGAGGCT GGAGCAAACC CCAACCAGCC AGACCGGGCA
    GGGCGCACCC CGCTTCACAC GGCTGTGGCT GCTGATGCTA GGGAGGTTTG CCAGCTCTTA
    CTCCGCAGCA GACAGACTGC AGTGGATGCA CGGACAGAGG ATGGGACCAC AGCCCTGATG
    CTGGCCGCCA GGCTGGCTGT CGAAGACCTG GTTGAAGAAC TGATTGCAGC CCAAGCAGAT
    GTGGGGGCCA GAGACAAATG GGGAAAAACA GCGCTGCACT GGGCCGCCGC CGTGAACAAC
    GCCCGGGCCG CCCGCTCCCT TCTCCAGGCG GGAGCCGATA AAGACGCACA GGATGGCAGG
    GAGCAGACGC CGCTGTTCCT GGCGGCCCGA GAAGGGGCGA TGGAGGTAGC TCAGCTGCTG
    CTGGGGCTGG GAGCCGCCCA AGGACTACGG GACCAGGCCG GCTTAACACC CGGAGACGTC
    GCCCGTCAGC GCAACCACTG GGATCTGCTG ACGCTGCTGG AAGGGGCGGG GCCACCGGAG
    GCGCGTCACA AACCCACGCC GGGCCGCGGC GCCGGCACCT TCCAGCGCGC GCGGACCGCG
    TCCGGAAGCG TGCCCCCGCG TGGGGGCGGG GCTGTGCCGC GCTGCCGGAC GCTGTCAGCC
    GGAGCCCGTC CGCAAGCCCG GACTTTGTCC GTAGACTTGG CCGCGCACGG GGGCGGGGCC
    TACTCGCACT GCCGGAGCCT ATCGAAAGGA ACAGGAGAAG GCCCGCCCCT CCGCGGCCGT
    AGGTTCTCAG CAGGCATGCG CGGGCCTCGG CCCAACCCTG CAATGGTGCG AGGAAGACCC
    GGGGTCTCGG CCGGGAGCGG GGGCGTGGCT GCAACTGTTG ACTGGCCCTG CGACTGGGTG
    CCCCTGGGAG CCTGCGGCCC CGCTTCCAAC ACGCCAATCC CGCCTCCTTG CCTAACACCG
    TCCCCTGAGC GGGGATCCCC TCAAGTTGCC TGGGGTCCCC CAGCCCACCA AGTAATACCC
    TTAAATGCAG GAGGCGAGGG TCAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229526
    Clone ID Related Accession (Same CDS sequence) XM_024983664.1
    Accession Version XM_024983664.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurogenic locus notch homolog protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCAGCCCC CTTCACTGCT GCTGCCACTG CTGCTGCTGC TGTGTCAGCC AGTCGTCAGG 
    ACCAGAGGGC TGCAGTGCGG GAGTTTCCCA GAACCCTGTG CCAACGGAGG CACCTGCCTG
    AGCCTATCCC AGGGGCAAGG GACCTGCCAG TGTGCCCCTG GCTTCCTGGG TGAGACATGC
    CAGTTTCCTG ACCCCTGCCA GGATGCCCAG CCCTGCCAGA ACGGAGGCAG CTGCCACACC
    TTTCTTCCCA CCCTTCCCGG CTCCCCTGGC ACCCCCTCTC CCATGGCCCC CAGCTTCTTC
    TGCACCTGCC CCTCTGGCTT CACTGGCGAT AGGTGCCAGG CCCAGATCAG GGACCCCTGT
    TCCTCCTTCT GTTCCAAAAT GGGTCGCTGC CACCTCCAGG ACTCGGGCCG CCCACGATGC
    TCCTGCATGC CAGGATGGAC GGGTGAGCAC TGCCAGCTTC GAGACTTCTG CTCAGCCAAC
    CCCTGCGTCA ATGGAGGAGT GTGTCTGGCC ACCTACCCCC AGATCCAGTG CCGCTGCCCA
    CCCGGCTTCG AGGGCCACGC CTGCGAACAC GACGTCAACG AGTGTTACCT GGACCCGGGA
    CCCTGCCCCA AGGGCACCAC CTGCCATAAC ACCCTGGGAT CCTTCCAGTG TCTCTGCCCT
    GCTGGGCGGG AGGGTCCACG CTGTGGACTC CGGCCGGGAC CCTGCACCCC CAGGGGCTGT
    CTCAATGGGG GCACCTGTCA ACTGGTTCCA GGGAGAGACT CCACTTTCCA CCTGTGCCTC
    TGTCCCCCAG GCTTCACAGG CCCAAGCTGT GAGGTGAATC CAGATGACTG TGCTGGGCAC
    CAGTGTCAGA ACGGGGGCAC CTGCCAGGAT GGGCTGGGCA CCTACACCTG CCTCTGCCCA
    GAGGCCTGGA CAGGCTGGGA TTGCTCTGAA GATGTGGACG AATGTGAGGT TCAGGGTCCC
    CCTCGCTGCA GAAATGGGGG TACCTGCCAG AACTCAGCTG GAGACTTCTA CTGTGTGTGT
    GTGAGTGGCT GGGGAGGCGC CGGCTGCGAA GAGAACCTGG ACGACTGTGT GGCCGCCACC
    TGTGCCCCCG GATCCACCTG CATTGACCGC GTGGGCTCCT TCTCCTGCCT CTGCCCGCCT
    GGGCGCACAG GCCTCCTGTG CCACATGGAG GACATGTGTC TGAGTCAGCC ATGCCATGAG
    GAAGCCCAGT GCAGCACCAA CCCCCTGAGT GGCTCCACAC TCTGCGTATG TCAGCCAGGC
    TACACAGGGC CCACCTGCCA CCAGGACCTG GACGAGTGTC AGATGGCTCA GCAAGGCCCC
    AGTCCCTGTG AACACGGCGG CTCCTGCCTC AACACGCCCG GCTCCTTCGA GTGCCTCTGT
    CCGCCTGGCT ACACGGGTTC CCGCTGCGAG GCCGATCACA ACGAGTGCCT GTCGCAGCCC
    TGCCACAGGG GCGGCACCTG CCTGGACCTG CTCGCCACCT TCCAGTGCCT CTGCCCACCA
    GGCTTAGAAG GGCAGCTCTG TGAGGTGGAG ATAGACGAGT GCGCCTCGGC CCCCTGCTTG
    AACCAGGCTG ACTGCCACGA CCTGCTCAAC GGCTTCCAGT GCATCTGCCA GCCAGGTTTC
    ACTGGCCCCC GGTGTGAGGA GGACATCAAC GAATGTCAAA GCTCTCCCTG TGCCAATGGC
    GGGGAGTGCC AGGACCAGCC TGGATCCTTC CATTGCAAGT GTCCCCCAGG CTTTGAAGGC
    CCACGCTGTC AGGAGGAGGT GGACGAGTGT CTGAGTGGCC CATGCCCCAC CGGAGCCAGC
    TGCCTTGACC TCCCAGGAGC TTTCCTTTGC GTCTGCCCCT CTGGCTTCAC AGGCCATCTC
    TGTGAAATTC CCCTGTGTGC CCCCAACCTG TGCCAGCCCA AACAAAAATG TCAGGATCAG
    GAGGACAATG CCCACTGCCT CTGCCCTGAT GGAAACCCAG GCTGTGTCCC CACAGAAGAC
    AACTGCACCT GCCACCATGG GCACTGCCAG AGATCTTCAT GTGTGTGTGA TGGGGGCTGG
    ACAGGACCAG AGTGTGACAC AGATCTGGGG GGCTGTGTCT CCACCCCCTG TGCCCACGGA
    GGGACCTGCC ACCCCCAGCC CTTTGGCTAC AACTGCACCT GCCCCACAGG TTACACAGGG
    CCCACTTGCA GTGAGGAGGT GACAGCCTGT CACTCAGGGC CCTGTCTCAA TGGTGGTTCC
    TGCAGCCCCA GCCCCGGGGG CTACACCTGC ACCTGCCCTC TGAGCCACAC GGGGCTCCGC
    TGCCAGACCA GCATTGACCA TTGTGCCTCT GCACTGTGCC TCAATGGGGG CACCTGTGTG
    AACAAGCCGG GCACCTTCTC CTGCCTCTGC ACCCCAGGCT TCCAGGGCCC ACGCTGCGAG
    GGAAGGACCC GTCCCAGCTG CGCAGACAGC CCCTGTAGGA ACATGGCAAC CTGCCAAGAC
    AGCCCTCAGG GTCCCCGCTG CCTCTGTCCC CCTGGCTACA CAGGAGGCAG CTGTCAGACG
    CTGATGGACT TATGCGCCCA GAAGCCCTGC CCACAAAATT CCCACTGCCT CCAGACTGGG
    CCCTCCTTCC AGTGCCTGTG CCTCCAGGGA TGGACTGGGC CTCTCTGCAA CCTGCCGCTG
    TCCTCCTGCC AGAAGGTTGC TCTGAGCCAA GGCACAGAAG TCTCTTCCCT GTGCCAGAAC
    GGAGGCGTCT GCATTGACAG CGGCCCCTCC CATTTCTGCC ACTGTCCCCC TGGATTCCAA
    GGCAGCATAT GCCAGGACCA GGTGAACCCC TGTGAGTCCA GGCCCTGCCA GCATGGGGCC
    ACCTGCGTGG CCCAACCCAA CGGTTATCTC TGCCAGTGTG CACCCGGCTA CAGTGGACAG
    AACTGCTCAG AGGAACCGGA CGCATGTCAA TCCCAGCCCT GTCACAACCA GGGGACCTGT
    ATCTCCAAAC CCGGAGGCTT CCACTGTGCC TGCCCGCCAG GCTTTGTGGG GCTGCGCTGT
    GAGGGGGACG TGGATGAGTG TCTGGACCGG CCCTGTCACC CCACAGGCAC TGCAGCCTGC
    CACTCTCTGG CCAATGCCTT CTACTGCCAG TGTCTGCCTG GACACACAGG CCAGTGGTGT
    GAAGTGGAGC TAGACCCCTG CCAGAGCCAG CCCTGTGCCC ACAGCGGGTC ATGTGAGGCC
    ACAGCAGGGC CACCCCCAGG ATTCACCTGC CACTGTCCCC AGGGTTTTGA AGGCCCCACC
    TGCAGCCACA GAGCCCCCTC CTGTGGTCTC CATCACTGCC ACCATGGTGG CCTGTGTCTG
    CCCTCCCCCA AGCCTGGACT CCCACCCCGC TGCGCCTGCC TCAATGGTTA TGGGGGTCCG
    GATTGCCTGA CCCCACCAGC TCCTTCAGGC TGTGGCCCTC CTTCTCCATG CCTACACAAT
    GGCAGCTGCT CAGAGACCCC CGGGCTGGGG GGCCCAGGCT TTCGCTGTTC CTGCCCTGCC
    AGCTCTCCAG GGCCCCGGTG TCAGAGGCCC GGAGTCAAGG GATGCGAGGG CAGAAGTGGA
    GATGGGGCCT GTGATGCTGG CTGCAGTGGC CCAGGAGGAA ACTGGGATGG GGGGGACTGC
    TCCCTGGGGG TCCCAGACCC CTGGAAGGGC TGCCCCTCCC ACTCCCGGTG CTGGCTTCTT
    TTCCGGGACG GGCAGTGCCA CCCACAGTGT GACTCTGAAG AGTGTTTGTT TGATGGCTAC
    GACTGTGAGA CTCCTCCAGC CTGCATTCCA GCCTATGACC AGTACTGCCG CGATCACTTC
    CACAATGGAC ACTGCGAGAA GGGCTGCAAC ACGGCAGAAT GTGGCTGGGA CGGGGGAGAC
    TGCAGGCCGG AAGATGGAAA TTCAGAGTGG GGGCCCTCCC TGGCCTTGTT GGTGGTGCTG
    AGCCCCTCAA CCCTGGACCA GCAGCTGCTC GCCCTGGCCC GGGTGCTGTC CCTGACTCTG
    AGGGTAGGGC TCTGGGTGAG GAAGGACAGT GAAGGCAAGG ACATGGTGTA CCCCTATCCT
    GGAGCCCAAG CAGAAGAGGA GCTAGGAGGA ACCCCAGACC CCTCTCAGCA GGAGAGAGCA
    GCCCCCCAAA CAGAGCCTAC AGGCAAGGAG ACAGATTCCC TGAGCACTGG GTTTGTGGTG
    GTGATGGGTG TGGACTTGTC CCACTGTGGC CCCGAGCGCC CTGCATCCCG CTGCCCCTGG
    GACCCTGGGC TCCTACTCCG CTTCCTTGCT GCAATGGCTG CAGTGGGGGC CCTGGAACCC
    CTACTGCCAG GACCCCTGCT GGCTGCCCAC CCTCGTGCAG GCACCAAGCC CTCCGCCAAC
    CAGCTTCCCT GGCCTGTACT GTGCTCCCCA GTGGTCGGGG TCCTTCTCCT AGCCCTCGGG
    GCTCTTCTCG TCCTCCAGCT CATCCGTCGC AAGCGCCGAG AGCATGGGGC CCTCTGGCTG
    CCCCCTGGGT TCACTCGCAG GCCCCGGACT CAGCCAGTTT CCCGCCGACG TCGACCCCCG
    CTGGGCGAGG ACAGCATCGG CCTCAAGGCA CTGAAAGCAG AGGCAGACAT GGATGAGGAT
    GGTGTTGTGA TGTGCTCAGT CCCCAAGGAA GGAGAGGAGG TGGGCCAGGC TGAAGAAATG
    GCCTCACCCC CCAAGTGCCA GCTCTGGTCT CTGAGCAGTG ACTGCCAAGA GCTCCCCCAG
    GCAGCCATGC TGACTCCTCC CCAGGATTCT GAGATGGATG TCCCTGACGT GGACACCCAG
    GGACCTGATG GGGTGACACC CCTGATGTCA GCAGTCTGCT GTGGGGGCGT GGAGTCCAGG
    ACCTACCAGG AGACATGGCT GGGAAGTCCT GAGCCCTGGG AACCTCTGCT GGAGGGAGGG
    GCCTGTCCCC AGGTTCACAC TGTGGGCACT GGGGAGACAC CCCTGCACCT GGCTGCTCGA
    TTCTCCCGGC CAACCGCTGC CCGCCGCCTC CTCGAGGCTG GAGCAAACCC CAACCAGCCA
    GACCGGGCAG GGCGCACCCC GCTTCACACG GCTGTGGCTG CTGATGCTAG GGAGGTTTGC
    CAGCTCTTAC TCCGCAGCAG ACAGACTGCA GTGGATGCAC GGACAGAGGA TGGGACCACA
    GCCCTGATGC TGGCCGCCAG GCTGGCTGTC GAAGACCTGG TTGAAGAACT GATTGCAGCC
    CAAGCAGATG TGGGGGCCAG AGACAAATGG GGAAAAACAG CGCTGCACTG GGCCGCCGCC
    GTGAACAACG CCCGGGCCGC CCGCTCCCTT CTCCAGGCGG GAGCCGATAA AGACGCACAG
    GATGGCAGGG AGCAGACGCC GCTGTTCCTG GCGGCCCGAG AAGGGGCGAT GGAGGTAGCT
    CAGCTGCTGC TGGGGCTGGG AGCCGCCCAA GGACTACGGG ACCAGGCCGG CTTAACACCC
    GGAGACGTCG CCCGTCAGCG CAACCACTGG GATCTGCTGA CGCTGCTGGA AGGGGCGGGG
    CCACCGGAGG CGCGTCACAA ACCCACGCCG GGCCGCGGCG CCGGCACCTT CCAGCGCGCG
    CGGACCGCGT CCGGAAGCGT GCCCCCGCGT GGGGGCGGGG CTGTGCCGCG CTGCCGGACG
    CTGTCAGCCG GAGCCCGTCC GCAAGCCCGG ACTTTGTCCG TAGACTTGGC CGCGCACGGG
    GGCGGGGCCT ACTCGCACTG CCGGAGCCTA TCGAAAGGAA CAGGAGAAGG CCCGCCCCTC
    CGCGGCCGTA GGTTCTCAGC AGGCATGCGC GGGCCTCGGC CCAACCCTGC AATGGTGCGA
    GGAAGACCCG GGGTCTCGGC CGGGAGCGGG GGCGTGGCTG CAACTGTTGA CTGGCCCTGC
    GACTGGGTGC CCCTGGGAGC CTGCGGCCCC GCTTCCAACA CGCCAATCCC GCCTCCTTGC
    CTAACACCGT CCCCTGAGCG GGGATCCCCT CAAGTTGCCT GGGGTCCCCC AGCCCACCAA
    GTAATACCCT TAAATGCAGG AGGCGAGGGT CAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839432.1
    CDS137..6115
    Translation

    Target ORF information:

    RefSeq Version XM_024983664.1
    Organism Bos taurus(cattle)
    Definition Bos taurus notch 4 (NOTCH4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983664.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCAGCCCC CTTCACTGCT GCTGCCACTG CTGCTGCTGC TGTGTCAGCC AGTCGTCAGG 
    ACCAGAGGGC TGCAGTGCGG GAGTTTCCCA GAACCCTGTG CCAACGGAGG CACCTGCCTG
    AGCCTATCCC AGGGGCAAGG GACCTGCCAG TGTGCCCCTG GCTTCCTGGG TGAGACATGC
    CAGTTTCCTG ACCCCTGCCA GGATGCCCAG CCCTGCCAGA ACGGAGGCAG CTGCCACACC
    TTTCTTCCCA CCCTTCCCGG CTCCCCTGGC ACCCCCTCTC CCATGGCCCC CAGCTTCTTC
    TGCACCTGCC CCTCTGGCTT CACTGGCGAT AGGTGCCAGG CCCAGATCAG GGACCCCTGT
    TCCTCCTTCT GTTCCAAAAT GGGTCGCTGC CACCTCCAGG ACTCGGGCCG CCCACGATGC
    TCCTGCATGC CAGGATGGAC GGGTGAGCAC TGCCAGCTTC GAGACTTCTG CTCAGCCAAC
    CCCTGCGTCA ATGGAGGAGT GTGTCTGGCC ACCTACCCCC AGATCCAGTG CCGCTGCCCA
    CCCGGCTTCG AGGGCCACGC CTGCGAACAC GACGTCAACG AGTGTTACCT GGACCCGGGA
    CCCTGCCCCA AGGGCACCAC CTGCCATAAC ACCCTGGGAT CCTTCCAGTG TCTCTGCCCT
    GCTGGGCGGG AGGGTCCACG CTGTGGACTC CGGCCGGGAC CCTGCACCCC CAGGGGCTGT
    CTCAATGGGG GCACCTGTCA ACTGGTTCCA GGGAGAGACT CCACTTTCCA CCTGTGCCTC
    TGTCCCCCAG GCTTCACAGG CCCAAGCTGT GAGGTGAATC CAGATGACTG TGCTGGGCAC
    CAGTGTCAGA ACGGGGGCAC CTGCCAGGAT GGGCTGGGCA CCTACACCTG CCTCTGCCCA
    GAGGCCTGGA CAGGCTGGGA TTGCTCTGAA GATGTGGACG AATGTGAGGT TCAGGGTCCC
    CCTCGCTGCA GAAATGGGGG TACCTGCCAG AACTCAGCTG GAGACTTCTA CTGTGTGTGT
    GTGAGTGGCT GGGGAGGCGC CGGCTGCGAA GAGAACCTGG ACGACTGTGT GGCCGCCACC
    TGTGCCCCCG GATCCACCTG CATTGACCGC GTGGGCTCCT TCTCCTGCCT CTGCCCGCCT
    GGGCGCACAG GCCTCCTGTG CCACATGGAG GACATGTGTC TGAGTCAGCC ATGCCATGAG
    GAAGCCCAGT GCAGCACCAA CCCCCTGAGT GGCTCCACAC TCTGCGTATG TCAGCCAGGC
    TACACAGGGC CCACCTGCCA CCAGGACCTG GACGAGTGTC AGATGGCTCA GCAAGGCCCC
    AGTCCCTGTG AACACGGCGG CTCCTGCCTC AACACGCCCG GCTCCTTCGA GTGCCTCTGT
    CCGCCTGGCT ACACGGGTTC CCGCTGCGAG GCCGATCACA ACGAGTGCCT GTCGCAGCCC
    TGCCACAGGG GCGGCACCTG CCTGGACCTG CTCGCCACCT TCCAGTGCCT CTGCCCACCA
    GGCTTAGAAG GGCAGCTCTG TGAGGTGGAG ATAGACGAGT GCGCCTCGGC CCCCTGCTTG
    AACCAGGCTG ACTGCCACGA CCTGCTCAAC GGCTTCCAGT GCATCTGCCA GCCAGGTTTC
    ACTGGCCCCC GGTGTGAGGA GGACATCAAC GAATGTCAAA GCTCTCCCTG TGCCAATGGC
    GGGGAGTGCC AGGACCAGCC TGGATCCTTC CATTGCAAGT GTCCCCCAGG CTTTGAAGGC
    CCACGCTGTC AGGAGGAGGT GGACGAGTGT CTGAGTGGCC CATGCCCCAC CGGAGCCAGC
    TGCCTTGACC TCCCAGGAGC TTTCCTTTGC GTCTGCCCCT CTGGCTTCAC AGGCCATCTC
    TGTGAAATTC CCCTGTGTGC CCCCAACCTG TGCCAGCCCA AACAAAAATG TCAGGATCAG
    GAGGACAATG CCCACTGCCT CTGCCCTGAT GGAAACCCAG GCTGTGTCCC CACAGAAGAC
    AACTGCACCT GCCACCATGG GCACTGCCAG AGATCTTCAT GTGTGTGTGA TGGGGGCTGG
    ACAGGACCAG AGTGTGACAC AGATCTGGGG GGCTGTGTCT CCACCCCCTG TGCCCACGGA
    GGGACCTGCC ACCCCCAGCC CTTTGGCTAC AACTGCACCT GCCCCACAGG TTACACAGGG
    CCCACTTGCA GTGAGGAGGT GACAGCCTGT CACTCAGGGC CCTGTCTCAA TGGTGGTTCC
    TGCAGCCCCA GCCCCGGGGG CTACACCTGC ACCTGCCCTC TGAGCCACAC GGGGCTCCGC
    TGCCAGACCA GCATTGACCA TTGTGCCTCT GCACTGTGCC TCAATGGGGG CACCTGTGTG
    AACAAGCCGG GCACCTTCTC CTGCCTCTGC ACCCCAGGCT TCCAGGGCCC ACGCTGCGAG
    GGAAGGACCC GTCCCAGCTG CGCAGACAGC CCCTGTAGGA ACATGGCAAC CTGCCAAGAC
    AGCCCTCAGG GTCCCCGCTG CCTCTGTCCC CCTGGCTACA CAGGAGGCAG CTGTCAGACG
    CTGATGGACT TATGCGCCCA GAAGCCCTGC CCACAAAATT CCCACTGCCT CCAGACTGGG
    CCCTCCTTCC AGTGCCTGTG CCTCCAGGGA TGGACTGGGC CTCTCTGCAA CCTGCCGCTG
    TCCTCCTGCC AGAAGGTTGC TCTGAGCCAA GGCACAGAAG TCTCTTCCCT GTGCCAGAAC
    GGAGGCGTCT GCATTGACAG CGGCCCCTCC CATTTCTGCC ACTGTCCCCC TGGATTCCAA
    GGCAGCATAT GCCAGGACCA GGTGAACCCC TGTGAGTCCA GGCCCTGCCA GCATGGGGCC
    ACCTGCGTGG CCCAACCCAA CGGTTATCTC TGCCAGTGTG CACCCGGCTA CAGTGGACAG
    AACTGCTCAG AGGAACCGGA CGCATGTCAA TCCCAGCCCT GTCACAACCA GGGGACCTGT
    ATCTCCAAAC CCGGAGGCTT CCACTGTGCC TGCCCGCCAG GCTTTGTGGG GCTGCGCTGT
    GAGGGGGACG TGGATGAGTG TCTGGACCGG CCCTGTCACC CCACAGGCAC TGCAGCCTGC
    CACTCTCTGG CCAATGCCTT CTACTGCCAG TGTCTGCCTG GACACACAGG CCAGTGGTGT
    GAAGTGGAGC TAGACCCCTG CCAGAGCCAG CCCTGTGCCC ACAGCGGGTC ATGTGAGGCC
    ACAGCAGGGC CACCCCCAGG ATTCACCTGC CACTGTCCCC AGGGTTTTGA AGGCCCCACC
    TGCAGCCACA GAGCCCCCTC CTGTGGTCTC CATCACTGCC ACCATGGTGG CCTGTGTCTG
    CCCTCCCCCA AGCCTGGACT CCCACCCCGC TGCGCCTGCC TCAATGGTTA TGGGGGTCCG
    GATTGCCTGA CCCCACCAGC TCCTTCAGGC TGTGGCCCTC CTTCTCCATG CCTACACAAT
    GGCAGCTGCT CAGAGACCCC CGGGCTGGGG GGCCCAGGCT TTCGCTGTTC CTGCCCTGCC
    AGCTCTCCAG GGCCCCGGTG TCAGAGGCCC GGAGTCAAGG GATGCGAGGG CAGAAGTGGA
    GATGGGGCCT GTGATGCTGG CTGCAGTGGC CCAGGAGGAA ACTGGGATGG GGGGGACTGC
    TCCCTGGGGG TCCCAGACCC CTGGAAGGGC TGCCCCTCCC ACTCCCGGTG CTGGCTTCTT
    TTCCGGGACG GGCAGTGCCA CCCACAGTGT GACTCTGAAG AGTGTTTGTT TGATGGCTAC
    GACTGTGAGA CTCCTCCAGC CTGCATTCCA GCCTATGACC AGTACTGCCG CGATCACTTC
    CACAATGGAC ACTGCGAGAA GGGCTGCAAC ACGGCAGAAT GTGGCTGGGA CGGGGGAGAC
    TGCAGGCCGG AAGATGGAAA TTCAGAGTGG GGGCCCTCCC TGGCCTTGTT GGTGGTGCTG
    AGCCCCTCAA CCCTGGACCA GCAGCTGCTC GCCCTGGCCC GGGTGCTGTC CCTGACTCTG
    AGGGTAGGGC TCTGGGTGAG GAAGGACAGT GAAGGCAAGG ACATGGTGTA CCCCTATCCT
    GGAGCCCAAG CAGAAGAGGA GCTAGGAGGA ACCCCAGACC CCTCTCAGCA GGAGAGAGCA
    GCCCCCCAAA CAGAGCCTAC AGGCAAGGAG ACAGATTCCC TGAGCACTGG GTTTGTGGTG
    GTGATGGGTG TGGACTTGTC CCACTGTGGC CCCGAGCGCC CTGCATCCCG CTGCCCCTGG
    GACCCTGGGC TCCTACTCCG CTTCCTTGCT GCAATGGCTG CAGTGGGGGC CCTGGAACCC
    CTACTGCCAG GACCCCTGCT GGCTGCCCAC CCTCGTGCAG GCACCAAGCC CTCCGCCAAC
    CAGCTTCCCT GGCCTGTACT GTGCTCCCCA GTGGTCGGGG TCCTTCTCCT AGCCCTCGGG
    GCTCTTCTCG TCCTCCAGCT CATCCGTCGC AAGCGCCGAG AGCATGGGGC CCTCTGGCTG
    CCCCCTGGGT TCACTCGCAG GCCCCGGACT CAGCCAGTTT CCCGCCGACG TCGACCCCCG
    CTGGGCGAGG ACAGCATCGG CCTCAAGGCA CTGAAAGCAG AGGCAGACAT GGATGAGGAT
    GGTGTTGTGA TGTGCTCAGT CCCCAAGGAA GGAGAGGAGG TGGGCCAGGC TGAAGAAATG
    GCCTCACCCC CCAAGTGCCA GCTCTGGTCT CTGAGCAGTG ACTGCCAAGA GCTCCCCCAG
    GCAGCCATGC TGACTCCTCC CCAGGATTCT GAGATGGATG TCCCTGACGT GGACACCCAG
    GGACCTGATG GGGTGACACC CCTGATGTCA GCAGTCTGCT GTGGGGGCGT GGAGTCCAGG
    ACCTACCAGG AGACATGGCT GGGAAGTCCT GAGCCCTGGG AACCTCTGCT GGAGGGAGGG
    GCCTGTCCCC AGGTTCACAC TGTGGGCACT GGGGAGACAC CCCTGCACCT GGCTGCTCGA
    TTCTCCCGGC CAACCGCTGC CCGCCGCCTC CTCGAGGCTG GAGCAAACCC CAACCAGCCA
    GACCGGGCAG GGCGCACCCC GCTTCACACG GCTGTGGCTG CTGATGCTAG GGAGGTTTGC
    CAGCTCTTAC TCCGCAGCAG ACAGACTGCA GTGGATGCAC GGACAGAGGA TGGGACCACA
    GCCCTGATGC TGGCCGCCAG GCTGGCTGTC GAAGACCTGG TTGAAGAACT GATTGCAGCC
    CAAGCAGATG TGGGGGCCAG AGACAAATGG GGAAAAACAG CGCTGCACTG GGCCGCCGCC
    GTGAACAACG CCCGGGCCGC CCGCTCCCTT CTCCAGGCGG GAGCCGATAA AGACGCACAG
    GATGGCAGGG AGCAGACGCC GCTGTTCCTG GCGGCCCGAG AAGGGGCGAT GGAGGTAGCT
    CAGCTGCTGC TGGGGCTGGG AGCCGCCCAA GGACTACGGG ACCAGGCCGG CTTAACACCC
    GGAGACGTCG CCCGTCAGCG CAACCACTGG GATCTGCTGA CGCTGCTGGA AGGGGCGGGG
    CCACCGGAGG CGCGTCACAA ACCCACGCCG GGCCGCGGCG CCGGCACCTT CCAGCGCGCG
    CGGACCGCGT CCGGAAGCGT GCCCCCGCGT GGGGGCGGGG CTGTGCCGCG CTGCCGGACG
    CTGTCAGCCG GAGCCCGTCC GCAAGCCCGG ACTTTGTCCG TAGACTTGGC CGCGCACGGG
    GGCGGGGCCT ACTCGCACTG CCGGAGCCTA TCGAAAGGAA CAGGAGAAGG CCCGCCCCTC
    CGCGGCCGTA GGTTCTCAGC AGGCATGCGC GGGCCTCGGC CCAACCCTGC AATGGTGCGA
    GGAAGACCCG GGGTCTCGGC CGGGAGCGGG GGCGTGGCTG CAACTGTTGA CTGGCCCTGC
    GACTGGGTGC CCCTGGGAGC CTGCGGCCCC GCTTCCAACA CGCCAATCCC GCCTCCTTGC
    CTAACACCGT CCCCTGAGCG GGGATCCCCT CAAGTTGCCT GGGGTCCCCC AGCCCACCAA
    GTAATACCCT TAAATGCAGG AGGCGAGGGT CAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32161
    Clone ID Related Accession (Same CDS sequence) XM_010818347.2
    Accession Version XM_010818347.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5322bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurogenic locus notch homolog protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818347.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCAGCCCC CTTCACTGCT GCTGCCACTG CTGCTGCTGC TGTGTCAGCC AGTCGTCAGG 
    ACCAGAGGGC TGCAGTGCGG GAGTTTCCCA GAACCCTGTG CCAACGGAGG CACCTGCCTG
    AGCCTATCCC AGGGGCAAGG GACCTGCCAG TGTGCCCCTG GCTTCCTGGG TGAGACATGC
    CAGTTTCCTG ACCCCTGCCA GGATGCCCAG CCCTGCCAGA ACGGAGGCAG CTGCCACACC
    TTTCTTCCCA CCCTTCCCGG CTCCCCTGGC ACCCCCTCTC CCATGGCCCC CAGCTTCTTC
    TGCACCTGCC CCTCTGGCTT CACTGGCGAT AGGTGCCAGG CCCAGATCAG GGACCCCTGT
    TCCTCCTTCT GTTCCAAAAT GGGTCGCTGC CACCTCCAGG ACTCGGGCCG CCCACGATGC
    TCCTGCATGC CAGGATGGAC GGGTGAGCAC TGCCAGCTTC GAGACTTCTG CTCAGCCAAC
    CCCTGCGTCA ATGGAGGAGT GTGTCTGGCC ACCTACCCCC AGATCCAGTG CCGCTGCCCA
    CCCGGCTTCG AGGGCCACGC CTGCGAACAC GACGTCAACG AGTGTTACCT GGACCCGGGA
    CCGTGCCCCA AGGGCACCAC CTGCCATAAC ACCCTGGGAT CCTGCCAGTG TCTCTGCCCT
    GCTGGGCGGG AGGGTCCACG CTGTGGACTC CGGCCGGGAC CCTGCACCCC CAGGGGCTGT
    CTCAATGGGG GCACCTGTCA ACTGGTTCCA GGGAGAGACT CCACTTTCCA CCTGTGCCTC
    TGTCCCCCAG GCTTCACAGG CCCAAGCTGT GAGGTGAATC CAGATGACTG TGCTGGGCAC
    CAGTGTCAGA ACGGGGGCAC CTGCCAGGAT GGGCTGGGCA CCTACACCTG CCTCTGCCCA
    GAGGCCTGGA CAGGCTGGGA TTGCTCTGAA GATGTGGACG AATGTGAGGT TCAGGGTCCC
    CCTCGCTGCA GAAATGGGGG TACCTGCCAG AACTCAGCTG GAGACTTCTA CTGTGTGTGT
    GTGAGTGGCT GGGGAGGCGC CGGCTGCGAA GAGAACCTGG ACGACTGTGT GGCCGCCACC
    TGTGCCCCCG GATCCACCTG CATTGACCGC GTGGGCTCCT TCTCCTGCCT CTGCCCGCCT
    GGGCGCACAG GCCTCCTGTG CCACATGGAG GACATGTGTC TGAGTCAGCC ATGCCATGAG
    GAAGCCCAGT GCAGCACCAA CCCCCTGAGT GGCTCCACAC TCTGCGTATG TCAGCCAGGC
    TACACAGGGC CCACCTGCCA CCAGGACCTG GACGAGTGTC AGATGGCTCA GCAAGGCCCC
    AGTCCCTGTG AACACGGCGG CTCCTGCCTC AACACGCCCG GCTCCTTCGA GTGCCTCTGT
    CCGCCTGGCT ACACGGGTTC CCGCTGCGAG GCCGATCACA ACGAGTGCCT GTCGCAGCCC
    TGCCACAGGG GCGGCACCTG CCTGGACCTG CTCGCCACCT TCCAGTGCCT CTGCCCACCA
    GGCTTAGAAG GGCAGCTCTG TGAGGTGGAG ATAGACGAGT GCGCCTCGGC CCCCTGCTTG
    AACCAGGCTG ACTGCCACGA CCTGCTCAAC GGCTTCCAGT GCATCTGCCA GCCAGGTTTC
    ACTGGCCCCC GGTGTGAGGA GGACATCAAC GAATGTCAAA GCTCTCCCTG TGCCAATGGC
    GGGGAGTGCC AGGACCAGCC TGGATCCTTC CATTGCAAGT GTCCCCCAGG CTTTGAAGGC
    CCACGCTGTC AGGAGGAGGT GGACGAGTGT CTGAGTGGCC CATGCCCCAC CGGAGCCAGC
    TGCCTTGACC TCCCAGGAGC TTTCCTTTGC GTCTGCCCCT CTGGCTTCAC AGGCCATCTC
    TGTGAAATTC CCCTGTGTGC CCCCAACCTG TGCCAGCCCA AACAAAAATG TCAGGATCAG
    GAGGACAATG CCCACTGCCT CTGCCCTGAT GGAAACCCAG GCTGTGTCCC CACAGAAGAC
    AACTGCACCT GCCACCATGG GCACTGCCAG AGATCTTCAT GTGTGTGTGA TGGGGGCTGG
    ACAGGACCAG AGTGTGACAC AGATCTGGGG GGCTGTGTCT CCACCCCCTG TGCCCACGGA
    GGGACCTGCC ACCCCCAGCC CTTTGGCTAC AACTGCACCT GCCCCACAGG TTACACAGGG
    CCCACTTGCA GTGAGGAGGT GACAGCCTGT CACTCAGGGC CCTGTCTCAA TGGTGGTTCC
    TGCAGCCCCA GCCCCGGGGG CTACACCTGC ACCTGCCCTC TGAGCCACAC GGGGCTCCGC
    TGCCAGACCA GCATTGACCA TTGTGCCTCT GCACTGTGCC TCAATGGGGG CACCTGTGTG
    AACAAGCCGG GCACCTTCTC CTGCCTCTGC ACCCCAGGCT TCCAGGGCCC ACGCTGCGAG
    GGAAGGACCC GTCCCAGCTG CGCAGACAGC CCCTGTAGGA ACATGGCAAC CTGCCAAGAC
    AGCCCTCAGG GTCCCCGCTG CCTCTGTCCC CCTGGCTACA CAGGAGGCAG CTGTCAGACG
    CTGATGGACT TATGCGCCCA GAAGCCCTGC CCACAAAATT CCCACTGCCT CCAGACTGGG
    CCCTCCTTCC AGTGCCTGTG CCTCCAGGGA TGGACTGGGC CTCTCTGCAA CCTGCCGCTG
    TCCTCCTGCC AGAAGGTTGC TCTGAGCCAA GGCACAGAAG TCTCTTCCCT GTGCCAGAAC
    GGAGGCGTCT GCATTGACAG CGGCCCCTCC CATTTCTGCC ACTGTCCCCC TGGATTCCAA
    GGCAGCATAT GCCAGGACCA GGTGAACCCC TGTGAGTCCA GGCCCTGCCA GCATGGGGCC
    ACCTGCGTGG CCCAACCCAA CGGTTATCTC TGCCAGTGTG CACCCGGCTA CAGTGGACAG
    AACTGCTCAG AGGAACCGGA CGCATGTCAA TCCCAGCCCT GTCACAACCA GGGGACCTGT
    ATCTCCAAAC CCGGAGGCTT CCACTGTGCC TGCCCGCCAG GCTTTGTGGG GCTGCGCTGT
    GAGGGGGACG TGGATGAGTG TCTGGACCGG CCCTGTCACC CCACAGGCAC TGCAGCCTGC
    CACTCTCTGG CCAATGCCTT CTACTGCCAG TGTCTGCCTG GACACACAGG CCAGTGGTGT
    GAAGTGGAGC TAGACCCCTG CCAGAGCCAG CCCTGTGCCC ACAGCGGGTC ATGTGAGGCC
    ACAGCAGGGC CACCCCCAGG ATTCACCTGC CACTGTCCCC AGGGTTTTGA AGGCCCCACC
    TGCAGCCACA GAGCCCCCTC CTGTGGTCTC CATCACTGCC ACCATGGTGG CCTGTGTCTG
    CCCTCCCCCA AGCCTGGACT CCCACCCCGC TGCGCCTGCC TCAATGGTTA TGGGGGTCCG
    GATTGCCTGA CCCCACCAGC TCCTTCAGGC TGTGGCCCTC CTTCTCCATG CCTACACAAT
    GGCAGCTGCT CAGAGACCCC CGGGCTGGGG GGCCCAGGCT TTCGCTGTTC CTGCCCTGCC
    AGCTCTCCAG GGCCCCGGTG TCAGAGGCCC GGAGTCAAGG GATGCGAGGG CAGAAGTGGA
    GATGGGGCCT GTGATGCTGG CTGCAGTGGC CCAGGAGGAA ACTGGGATGG GGGGGACTGC
    TCCCTGGGGG TCCCAGACCC CTGGAAGGGC TGCCCCTCCC ACTCCCGGTG CTGGCTTCTT
    TTCCGGGACG GGCAGTGCCA CCCACAGTGT GACTCTGAAG AGTGTTTGTT TGATGGCTAC
    GACTGTGAGA CTCCTCCAGC CTGCATTCCA GCCTATGACC AGTACTGCCG CGATCACTTC
    CACAATGGAC ACTGCGAGAA GGGCTGCAAC ACGGCAGAAT GTGGCTGGGA CGGGGGAGAC
    TGCAGGCCGG AAGATGGAAA TTCAGAGTGG GGGCCCTCCC TGGCCTTGTT GGTGGTGCTG
    AGCCCCTCAA CCCTGGACCA GCAGCTGCTC GCCCTGGCCC GGGTGCTGTC CCTGACTCTG
    AGGGTAGGGC TCTGGGTGAG GAAGGACAGT GAAGGCAAGG ACATGGTGTA CCCCTATCCT
    GGAGCCCAAG CAGAAGAGGA GCTAGGAGGA ACCCCAGACC CCTCTCAGCA GGAGAGAGCA
    GCCCCCCAAA CAGAGCCTAC AGGCAAGGAG ACAGATTCCC TGAGCACTGG GTTTGTGGTG
    GTGATGGGTG TGGACTTGTC CCACTGTGGC CCCGAGCGCC CTGCATCCCG CTGCCCCTGG
    GACCCTGGGC TCCTACTCCG CTTCCTTGCT GCAATGGCTG CAGTGGGGGC CCTGGAACCC
    CTACTGCCAG GACCCCTGCT GGCTGCCCAC CCTCGTGCAG GCACCAAGCC CTCCGCCAAC
    CAGCTTCCCT GGCCTGTACT GTGCTCCCCA GTGGTCGGGG TCCTTCTCCT AGCCCTCGGG
    GCTCTTCTCG TCCTCCAGCT CATCCGTCGC AAGCGCCGAG AGCATGGGGC CCTCTGGCTG
    CCCCCTGGGT TCACTCGCAG GCCCCGGACT CAGCCAGTTT CCCGCCGACG TCGACCCCCG
    CTGGGCGAGG ACAGCATCGG CCTCAAGGCA CTGAAAGCAG AGGCAGACAT GGATGAGGAT
    GGTGTTGTGA TGTGCTCAGT CCCCAAGGAA GGAGAGGAGG TGGGCCAGGC TGAAGAAATG
    GCCTCACCCC CCAAGTGCCA GCTCTGGTCT CTGAGCAGTG ACTGCCAAGA GCTCCCCCAG
    GCAGCCATGC TGACTCCTCC CCAGGATTCT GAGATGGATG TCCCTGACGT GGACACCCAG
    GGACCTGATG GGGTGACACC CCTGATGTCA GCAGTCTGCT GTGGGGGCGT GGAGTCCAGG
    ACCTACCAGG AGACATGGCT GGGAAGTCCT GAGCCCTGGG AACCTCTGCT GGAGGGAGGG
    GCCTGTCCCC AGGTTCACAC TGTGGGCACT GGGGAGACAC CCCTGCACCT GGCTGCTCGA
    TTCTCCCGGC CAACCGCTGC CCGCCGCCTC CTCGAGGCTG GAGCAAACCC CAACCAGCCA
    GACCGGGCAG GGCGCACCCC GCTTCACACG GCTGTGGCTG CTGATGCTAG GGAGGTTTGC
    CAGCTCTTAC TCCGCAGCAG ACAGACTGCA GTGGATGCAC GGACAGAGGA TGGGACCACA
    GCCCTGATGC TGGCCGCCAG GCTGGCTGTC GAAGACCTGG TTGAAGAACT GATTGCAGCC
    CAAGCAGATG TGGGGGCCAG AGACAAATGG GGGGCAACGC TTCCTGCAGG CCGACGGTCA
    CCCGGACAAC GCTTCCGCCT CAGAGGGACC CCGCGGGCGG AACAGCCCTT CCCCCAAGAC
    CAGAACCCGT GGGAAACAGG AACCCAGGCG TCTGGCCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816649.1
    CDS131..5452
    Translation

    Target ORF information:

    RefSeq Version XM_010818347.2
    Organism Bos taurus(cattle)
    Definition Bos taurus notch 4 (NOTCH4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818347.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCAGCCCC CTTCACTGCT GCTGCCACTG CTGCTGCTGC TGTGTCAGCC AGTCGTCAGG 
    ACCAGAGGGC TGCAGTGCGG GAGTTTCCCA GAACCCTGTG CCAACGGAGG CACCTGCCTG
    AGCCTATCCC AGGGGCAAGG GACCTGCCAG TGTGCCCCTG GCTTCCTGGG TGAGACATGC
    CAGTTTCCTG ACCCCTGCCA GGATGCCCAG CCCTGCCAGA ACGGAGGCAG CTGCCACACC
    TTTCTTCCCA CCCTTCCCGG CTCCCCTGGC ACCCCCTCTC CCATGGCCCC CAGCTTCTTC
    TGCACCTGCC CCTCTGGCTT CACTGGCGAT AGGTGCCAGG CCCAGATCAG GGACCCCTGT
    TCCTCCTTCT GTTCCAAAAT GGGTCGCTGC CACCTCCAGG ACTCGGGCCG CCCACGATGC
    TCCTGCATGC CAGGATGGAC GGGTGAGCAC TGCCAGCTTC GAGACTTCTG CTCAGCCAAC
    CCCTGCGTCA ATGGAGGAGT GTGTCTGGCC ACCTACCCCC AGATCCAGTG CCGCTGCCCA
    CCCGGCTTCG AGGGCCACGC CTGCGAACAC GACGTCAACG AGTGTTACCT GGACCCGGGA
    CCGTGCCCCA AGGGCACCAC CTGCCATAAC ACCCTGGGAT CCTGCCAGTG TCTCTGCCCT
    GCTGGGCGGG AGGGTCCACG CTGTGGACTC CGGCCGGGAC CCTGCACCCC CAGGGGCTGT
    CTCAATGGGG GCACCTGTCA ACTGGTTCCA GGGAGAGACT CCACTTTCCA CCTGTGCCTC
    TGTCCCCCAG GCTTCACAGG CCCAAGCTGT GAGGTGAATC CAGATGACTG TGCTGGGCAC
    CAGTGTCAGA ACGGGGGCAC CTGCCAGGAT GGGCTGGGCA CCTACACCTG CCTCTGCCCA
    GAGGCCTGGA CAGGCTGGGA TTGCTCTGAA GATGTGGACG AATGTGAGGT TCAGGGTCCC
    CCTCGCTGCA GAAATGGGGG TACCTGCCAG AACTCAGCTG GAGACTTCTA CTGTGTGTGT
    GTGAGTGGCT GGGGAGGCGC CGGCTGCGAA GAGAACCTGG ACGACTGTGT GGCCGCCACC
    TGTGCCCCCG GATCCACCTG CATTGACCGC GTGGGCTCCT TCTCCTGCCT CTGCCCGCCT
    GGGCGCACAG GCCTCCTGTG CCACATGGAG GACATGTGTC TGAGTCAGCC ATGCCATGAG
    GAAGCCCAGT GCAGCACCAA CCCCCTGAGT GGCTCCACAC TCTGCGTATG TCAGCCAGGC
    TACACAGGGC CCACCTGCCA CCAGGACCTG GACGAGTGTC AGATGGCTCA GCAAGGCCCC
    AGTCCCTGTG AACACGGCGG CTCCTGCCTC AACACGCCCG GCTCCTTCGA GTGCCTCTGT
    CCGCCTGGCT ACACGGGTTC CCGCTGCGAG GCCGATCACA ACGAGTGCCT GTCGCAGCCC
    TGCCACAGGG GCGGCACCTG CCTGGACCTG CTCGCCACCT TCCAGTGCCT CTGCCCACCA
    GGCTTAGAAG GGCAGCTCTG TGAGGTGGAG ATAGACGAGT GCGCCTCGGC CCCCTGCTTG
    AACCAGGCTG ACTGCCACGA CCTGCTCAAC GGCTTCCAGT GCATCTGCCA GCCAGGTTTC
    ACTGGCCCCC GGTGTGAGGA GGACATCAAC GAATGTCAAA GCTCTCCCTG TGCCAATGGC
    GGGGAGTGCC AGGACCAGCC TGGATCCTTC CATTGCAAGT GTCCCCCAGG CTTTGAAGGC
    CCACGCTGTC AGGAGGAGGT GGACGAGTGT CTGAGTGGCC CATGCCCCAC CGGAGCCAGC
    TGCCTTGACC TCCCAGGAGC TTTCCTTTGC GTCTGCCCCT CTGGCTTCAC AGGCCATCTC
    TGTGAAATTC CCCTGTGTGC CCCCAACCTG TGCCAGCCCA AACAAAAATG TCAGGATCAG
    GAGGACAATG CCCACTGCCT CTGCCCTGAT GGAAACCCAG GCTGTGTCCC CACAGAAGAC
    AACTGCACCT GCCACCATGG GCACTGCCAG AGATCTTCAT GTGTGTGTGA TGGGGGCTGG
    ACAGGACCAG AGTGTGACAC AGATCTGGGG GGCTGTGTCT CCACCCCCTG TGCCCACGGA
    GGGACCTGCC ACCCCCAGCC CTTTGGCTAC AACTGCACCT GCCCCACAGG TTACACAGGG
    CCCACTTGCA GTGAGGAGGT GACAGCCTGT CACTCAGGGC CCTGTCTCAA TGGTGGTTCC
    TGCAGCCCCA GCCCCGGGGG CTACACCTGC ACCTGCCCTC TGAGCCACAC GGGGCTCCGC
    TGCCAGACCA GCATTGACCA TTGTGCCTCT GCACTGTGCC TCAATGGGGG CACCTGTGTG
    AACAAGCCGG GCACCTTCTC CTGCCTCTGC ACCCCAGGCT TCCAGGGCCC ACGCTGCGAG
    GGAAGGACCC GTCCCAGCTG CGCAGACAGC CCCTGTAGGA ACATGGCAAC CTGCCAAGAC
    AGCCCTCAGG GTCCCCGCTG CCTCTGTCCC CCTGGCTACA CAGGAGGCAG CTGTCAGACG
    CTGATGGACT TATGCGCCCA GAAGCCCTGC CCACAAAATT CCCACTGCCT CCAGACTGGG
    CCCTCCTTCC AGTGCCTGTG CCTCCAGGGA TGGACTGGGC CTCTCTGCAA CCTGCCGCTG
    TCCTCCTGCC AGAAGGTTGC TCTGAGCCAA GGCACAGAAG TCTCTTCCCT GTGCCAGAAC
    GGAGGCGTCT GCATTGACAG CGGCCCCTCC CATTTCTGCC ACTGTCCCCC TGGATTCCAA
    GGCAGCATAT GCCAGGACCA GGTGAACCCC TGTGAGTCCA GGCCCTGCCA GCATGGGGCC
    ACCTGCGTGG CCCAACCCAA CGGTTATCTC TGCCAGTGTG CACCCGGCTA CAGTGGACAG
    AACTGCTCAG AGGAACCGGA CGCATGTCAA TCCCAGCCCT GTCACAACCA GGGGACCTGT
    ATCTCCAAAC CCGGAGGCTT CCACTGTGCC TGCCCGCCAG GCTTTGTGGG GCTGCGCTGT
    GAGGGGGACG TGGATGAGTG TCTGGACCGG CCCTGTCACC CCACAGGCAC TGCAGCCTGC
    CACTCTCTGG CCAATGCCTT CTACTGCCAG TGTCTGCCTG GACACACAGG CCAGTGGTGT
    GAAGTGGAGC TAGACCCCTG CCAGAGCCAG CCCTGTGCCC ACAGCGGGTC ATGTGAGGCC
    ACAGCAGGGC CACCCCCAGG ATTCACCTGC CACTGTCCCC AGGGTTTTGA AGGCCCCACC
    TGCAGCCACA GAGCCCCCTC CTGTGGTCTC CATCACTGCC ACCATGGTGG CCTGTGTCTG
    CCCTCCCCCA AGCCTGGACT CCCACCCCGC TGCGCCTGCC TCAATGGTTA TGGGGGTCCG
    GATTGCCTGA CCCCACCAGC TCCTTCAGGC TGTGGCCCTC CTTCTCCATG CCTACACAAT
    GGCAGCTGCT CAGAGACCCC CGGGCTGGGG GGCCCAGGCT TTCGCTGTTC CTGCCCTGCC
    AGCTCTCCAG GGCCCCGGTG TCAGAGGCCC GGAGTCAAGG GATGCGAGGG CAGAAGTGGA
    GATGGGGCCT GTGATGCTGG CTGCAGTGGC CCAGGAGGAA ACTGGGATGG GGGGGACTGC
    TCCCTGGGGG TCCCAGACCC CTGGAAGGGC TGCCCCTCCC ACTCCCGGTG CTGGCTTCTT
    TTCCGGGACG GGCAGTGCCA CCCACAGTGT GACTCTGAAG AGTGTTTGTT TGATGGCTAC
    GACTGTGAGA CTCCTCCAGC CTGCATTCCA GCCTATGACC AGTACTGCCG CGATCACTTC
    CACAATGGAC ACTGCGAGAA GGGCTGCAAC ACGGCAGAAT GTGGCTGGGA CGGGGGAGAC
    TGCAGGCCGG AAGATGGAAA TTCAGAGTGG GGGCCCTCCC TGGCCTTGTT GGTGGTGCTG
    AGCCCCTCAA CCCTGGACCA GCAGCTGCTC GCCCTGGCCC GGGTGCTGTC CCTGACTCTG
    AGGGTAGGGC TCTGGGTGAG GAAGGACAGT GAAGGCAAGG ACATGGTGTA CCCCTATCCT
    GGAGCCCAAG CAGAAGAGGA GCTAGGAGGA ACCCCAGACC CCTCTCAGCA GGAGAGAGCA
    GCCCCCCAAA CAGAGCCTAC AGGCAAGGAG ACAGATTCCC TGAGCACTGG GTTTGTGGTG
    GTGATGGGTG TGGACTTGTC CCACTGTGGC CCCGAGCGCC CTGCATCCCG CTGCCCCTGG
    GACCCTGGGC TCCTACTCCG CTTCCTTGCT GCAATGGCTG CAGTGGGGGC CCTGGAACCC
    CTACTGCCAG GACCCCTGCT GGCTGCCCAC CCTCGTGCAG GCACCAAGCC CTCCGCCAAC
    CAGCTTCCCT GGCCTGTACT GTGCTCCCCA GTGGTCGGGG TCCTTCTCCT AGCCCTCGGG
    GCTCTTCTCG TCCTCCAGCT CATCCGTCGC AAGCGCCGAG AGCATGGGGC CCTCTGGCTG
    CCCCCTGGGT TCACTCGCAG GCCCCGGACT CAGCCAGTTT CCCGCCGACG TCGACCCCCG
    CTGGGCGAGG ACAGCATCGG CCTCAAGGCA CTGAAAGCAG AGGCAGACAT GGATGAGGAT
    GGTGTTGTGA TGTGCTCAGT CCCCAAGGAA GGAGAGGAGG TGGGCCAGGC TGAAGAAATG
    GCCTCACCCC CCAAGTGCCA GCTCTGGTCT CTGAGCAGTG ACTGCCAAGA GCTCCCCCAG
    GCAGCCATGC TGACTCCTCC CCAGGATTCT GAGATGGATG TCCCTGACGT GGACACCCAG
    GGACCTGATG GGGTGACACC CCTGATGTCA GCAGTCTGCT GTGGGGGCGT GGAGTCCAGG
    ACCTACCAGG AGACATGGCT GGGAAGTCCT GAGCCCTGGG AACCTCTGCT GGAGGGAGGG
    GCCTGTCCCC AGGTTCACAC TGTGGGCACT GGGGAGACAC CCCTGCACCT GGCTGCTCGA
    TTCTCCCGGC CAACCGCTGC CCGCCGCCTC CTCGAGGCTG GAGCAAACCC CAACCAGCCA
    GACCGGGCAG GGCGCACCCC GCTTCACACG GCTGTGGCTG CTGATGCTAG GGAGGTTTGC
    CAGCTCTTAC TCCGCAGCAG ACAGACTGCA GTGGATGCAC GGACAGAGGA TGGGACCACA
    GCCCTGATGC TGGCCGCCAG GCTGGCTGTC GAAGACCTGG TTGAAGAACT GATTGCAGCC
    CAAGCAGATG TGGGGGCCAG AGACAAATGG GGGGCAACGC TTCCTGCAGG CCGACGGTCA
    CCCGGACAAC GCTTCCGCCT CAGAGGGACC CCGCGGGCGG AACAGCCCTT CCCCCAAGAC
    CAGAACCCGT GGGAAACAGG AACCCAGGCG TCTGGCCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38379
    Clone ID Related Accession (Same CDS sequence) NM_001206948.1
    Accession Version NM_001206948.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product neurogenic locus notch homolog protein 4 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02055356.1 and DAAA02055357.1. On or before May 13, 2011 this sequence version replaced XM_001788629.1, XM_002697367.1, XM_617999.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822090 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCAGCCCC CTTCACTGCT GCTGCCACTG CTGCTGCTGC TGTGTCAGCC AGTCGTCAGG 
    ACCAGAGGGC TGCAGTGCGG GAGTTTCCCA GAACCCTGTG CCAACGGAGG CACCTGCCTG
    AGCCTATCCC AGGGGCAAGG GACCTGCCAG TGTGCCCCTG GCTTCCTGGG TGAGACATGC
    CAGTTTCCTG ACCCCTGCCA GGATGCCCAG CCCTGCCAGA ACGGAGGCAG CTGCCACACC
    TTTCTTCCCA CCCTTCCCGG CTCCCCTGGC ACCCCCTCTC CCATGGCCCC CAGCTTCTTC
    TGCACCTGCC CCTCTGGCTT CACTGGCGAT AGGTGCCAGG CCCAGATCAG GGACCCCTGT
    TCCTCCTTCT GTTCCAAAAT GGGTCGCTGC CACCTCCAGG ACTCGGGCCG CCCACGATGC
    TCCTGCATGC CAGGATGGAC GGGTGAGCAC TGCCAGCTTC GAGACTTCTG CTCAGCCAAC
    CCCTGCGTCA ATGGAGGAGT GTGTCTGGCC ACCTACCCCC AGATCCAGTG CCGCTGCCCA
    CCCGGCTTCG AGGGCCACGC CTGCGAACAC GACGTCAACG AGTGTTACCT GGACCCGGGA
    CCGTGCCCCA AGGGCACCAC CTGCCATAAC ACCCTGGGAT CCTGCCAGTG TCTCTGCCCT
    GCTGGGCGGG AGGGTCCACG CTGTGGACTC CGGCCGGGAC CCTGCACCCC CAGGGGCTGT
    CTCAATGGGG GCACCTGTCA ACTGGTTCCA GGGAGAGACT CCACTTTCCA CCTGTGCCTC
    TGTCCCCCAG GCTTCACAGG CCCAAGCTGT GAGGTGAATC CAGATGACTG TGCTGGGCAC
    CAGTGTCAGA ACGGGGGCAC CTGCCAGGAT GGGCTGGGCA CCTACACCTG CCTCTGCCCA
    GAGGCCTGGA CAGGCTGGGA TTGCTCTGAA GATGTGGACG AATGTGAGGT TCAGGGTCCC
    CCTCGCTGCA GAAATGGGGG TACCTGCCAG AACTCAGCTG GAGACTTCTA CTGTGTGTGT
    GTGAGTGGCT GGGGAGGCGC CGGCTGCGAA GAGAACCTGG ACGACTGTGT GGCCGCCACC
    TGTGCCCCCG GATCCACCTG CATTGACCGC GTGGGCTCCT TCTCCTGCCT CTGCCCGCCT
    GGGCGCACAG GCCTCCTGTG CCACATGGAG GACATGTGTC TGAGTCAGCC ATGCCATGAG
    GAAGCCCAGT GCAGCACCAA CCCCCTGAGT GGCTCCACAC TCTGCGTATG TCAGCCAGGC
    TACACAGGGC CCACCTGCCA CCAGGACCTG GACGAGTGTC AGATGGCTCA GCAAGGCCCC
    AGTCCCTGTG AACACGGCGG CTCCTGCCTC AACACGCCCG GCTCCTTCGA GTGCCTCTGT
    CCGCCTGGCT ACACGGGTTC CCGCTGCGAG GCCGATCACA ACGAGTGCCT GTCGCAGCCC
    TGCCACAGGG GCGGCACCTG CCTGGACCTG CTCGCCACCT TCCAGTGCCT CTGCCCACCA
    GGCTTAGAAG GGCAGCTCTG TGAGGTGGAG ATAGACGAGT GCGCCTCGGC CCCCTGCTTG
    AACCAGGCTG ACTGCCACGA CCTGCTCAAC GGCTTCCAGT GCATCTGCCA GCCAGGTTTC
    ACTGGCCCCC GGTGTGAGGA GGACATCAAC GAATGTCAAA GCTCTCCCTG TGCCAATGGC
    GGGGAGTGCC AGGACCAGCC TGGATCCTTC CATTGCAAGT GTCCCCCAGG CTTTGAAGGC
    CCACGCTGTC AGGAGGAGGT GGACGAGTGT CTGAGTGGCC CATGCCCCAC CGGAGCCAGC
    TGCCTTGACC TCCCAGGAGC TTTCCTTTGC GTCTGCCCCT CTGGCTTCAC AGGCCATCTC
    TGTGAAATTC CCCTGTGTGC CCCCAACCTG TGCCAGCCCA AACAAAAATG TCAGGATCAG
    GAGGACAATG CCCACTGCCT CTGCCCTGAT GGAAACCCAG GCTGTGTCCC CACAGAAGAC
    AACTGCACCT GCCACCATGG GCACTGCCAG AGATCTTCAT GTGTGTGTGA TGGGGGCTGG
    ACAGGACCAG AGTGTGACAC AGATCTGGGG GGCTGTGTCT CCACCCCCTG TGCCCACGGA
    GGGACCTGCC ACCCCCAGCC CTTTGGCTAC AACTGCACCT GCCCCACAGG TTACACAGGG
    CCCACTTGCA GTGAGGAGGT GACAGCCTGT CACTCAGGGC CCTGTCTCAA TGGTGGTTCC
    TGCAGCCCCA GCCCCGGGGG CTACACCTGC ACCTGCCCTC TGAGCCACAC GGGGCTCCGC
    TGCCAGACCA GCATTGACCA TTGTGCCTCT GCACTGTGCC TCAATGGGGG CACCTGTGTG
    AACAAGCCGG GCACCTTCTC CTGCCTCTGC ACCCCAGGCT TCCAGGGCCC ACGCTGCGAG
    GGAAGGACCC GTCCCAGCTG CGCAGACAGC CCCTGTAGGA ACATGGCAAC CTGCCAAGAC
    AGCCCTCAGG GTCCCCGCTG CCTCTGTCCC CCTGGCTACA CAGGAGGCAG CTGTCAGACG
    CTGATGGACT TATGCGCCCA GAAGCCCTGC CCACAAAATT CCCACTGCCT CCAGACTGGG
    CCCTCCTTCC AGTGCCTGTG CCTCCAGGGA TGGACTGGGC CTCTCTGCAA CCTGCCGCTG
    TCCTCCTGCC AGAAGGTTGC TCTGAGCCAA GGCACAGAAG TCTCTTCCCT GTGCCAGAAC
    GGAGGCGTCT GCATTGACAG CGGCCCCTCC CATTTCTGCC ACTGTCCCCC TGGATTCCAA
    GGCAGCATAT GCCAGGACCA GGTGAACCCC TGTGAGTCCA GGCCCTGCCA GCATGGGGCC
    ACCTGCGTGG CCCAACCCAA CGGTTATCTC TGCCAGTGTG CACCCGGCTA CAGTGGACAG
    AACTGCTCAG AGGAACCGGA CGCATGTCAA TCCCAGCCCT GTCACAACCA GGGGACCTGT
    ATCTCCAAAC CCGGAGGCTT CCACTGTGCC TGCCCGCCAG GCTTTGTGGG GCTGCGCTGT
    GAGGGGGACG TGGATGAGTG TCTGGACCGG CCCTGTCACC CCACAGGCAC TGCAGCCTGC
    CACTCTCTGG CCAATGCCTT CTACTGCCAG TGTCTGCCTG GACACACAGG CCAGTGGTGT
    GAAGTGGAGC TAGACCCCTG CCAGAGCCAG CCCTGTGCCC ACAGCGGGTC ATGTGAGGCC
    ACAGCAGGGC CACCCCCAGG ATTCACCTGC CACTGTCCCC AGGGTTTTGA AGGCCCCACC
    TGCAGCCACA GAGCCCCCTC CTGTGGTCTC CATCACTGCC ACCATGGTGG CCTGTGTCTG
    CCCTCCCCCA AGCCTGGACT CCCACCCCGC TGCGCCTGCC TCAATGGTTA TGGGGGTCCG
    GATTGCCTGA CCCCACCAGC TCCTTCAGGC TGTGGCCCTC CTTCTCCATG CCTACACAAT
    GGCAGCTGCT CAGAGACCCC CGGGCTGGGG GGCCCAGGCT TTCGCTGTTC CTGCCCTGCC
    AGCTCTCCAG GGCCCCGGTG TCAGAGGCCC GGAGTCAAGG GATGCGAGGG CAGAAGTGGA
    GATGGGGCCT GTGATGCTGG CTGCAGTGGC CCAGGAGGAA ACTGGGATGG GGGGGACTGC
    TCCCTGGGGG TCCCAGACCC CTGGAAGGGC TGCCCCTCCC ACTCCCGGTG CTGGCTTCTT
    TTCCGGGACG GGCAGTGCCA CCCACAGTGT GACTCTGAAG AGTGTTTGTT TGATGGCTAC
    GACTGTGAGA CTCCTCCAGC CTGCATTCCA GCCTATGACC AGTACTGCCG CGATCACTTC
    CACAATGGAC ACTGCGAGAA GGGCTGCAAC ACGGCAGAAT GTGGCTGGGA CGGGGGAGAC
    TGCAGGCCGG AAGATGGAAA TTCAGAGTGG GGGCCCTCCC TGGCCTTGTT GGTGGTGCTG
    AGCCCCTCAA CCCTGGACCA GCAGCTGCTC GCCCTGGCCC GGGTGCTGTC CCTGACTCTG
    AGGGTAGGGC TCTGGGTGAG GAAGGACAGT GAAGGCAAGG ACATGGTGTA CCCCTATCCT
    GGAGCCCAAG CAGAAGAGGA GCTAGGAGGA ACCCCAGACC CCTCTCAGCA GGAGAGAGCA
    GCCCCCCAAA CAGAGCCTAC AGGCAAGGAG ACAGATTCCC TGAGCACTGG GTTTGTGGTG
    GTGATGGGTG TGGACTTGTC CCACTGTGGC CCCGAGCGCC CTGCATCCCG CTGCCCCTGG
    GACCCTGGGC TCCTACTCCG CTTCCTTGCT GCAATGGCTG CAGTGGGGGC CCTGGAACCC
    CTACTGCCAG GACCCCTGCT GGCTGCCCAC CCTCGTGCAG GCACCAAGCC CTCCGCCAAC
    CAGCTTCCCT GGCCTGTACT GTGCTCCCCA GTGGTCGGGG TCCTTCTCCT AGCCCTCGGG
    GCTCTTCTCG TCCTCCAGCT CATCCGTCGC AAGCGCCGAG AGCATGGGGC CCTCTGGCTG
    CCCCCTGGGT TCACTCGCAG GCCCCGGACT CAGCCAGTTT CCCGCCGACG TCGACCCCCG
    CTGGGCGAGG ACAGCATCGG CCTCAAGGCA CTGAAAGCAG AGGCAGACAT GGATGAGGAT
    GGTGTTGTGA TGTGCTCAGT CCCCAAGGAA GGAGAGGAGG CTGAAGAAAT GGCCTCACCC
    CCCAAGTGCC AGCTCTGGTC TCTGAGCAGT GACTGCCAAG AGCTCCCCCA GGCAGCCATG
    CTGACTCCTC CCCAGGATTC TGAGATGGAT GTCCCTGACG TGGACACCCA GGGACCTGAT
    GGGGTGACAC CCCTGATGTC AGCAGTCTGC TGTGGGGGCG TGGAGTCCAG GACCTACCAG
    GAGACATGGC TGGGAAGTCC TGAGCCCTGG GAACCTCTGC TGGAGGGAGG GGCCTGTCCC
    CAGGTTCACA CTGTGGGCAC TGGGGAGACA CCCCTGCACC TGGCTGCTCG ATTCTCCCGG
    CCAACCGCTG CCCGCCGCCT CCTCGAGGCT GGAGCAAACC CCAACCAGCC AGACCGGGCA
    GGGCGCACCC CGCTTCACAC GGCTGTGGCT GCTGATGCTA GGGAGGTTTG CCAGCTCTTA
    CTCCGCAGCA GACAGACTGC AGTGGATGCA CGGACAGAGG ATGGGACCAC AGCCCTGATG
    CTGGCCGCCA GGCTGGCTGT CGAAGACCTG GTTGAAGAAC TGATTGCAGC CCAAGCAGAT
    GTGGGGGCCA GAGACAAATG GGGAAAAACA GCGCTGCACT GGGCCGCCGC CGTGAACAAC
    GCCCGGGCCG CCCGCTCCCT TCTCCAGGCG GGAGCCGATA AAGACGCACA GGATGGCAGG
    GAGCAGACGC CGCTGTTCCT GGCGGCCCGA GAAGGGGCGA TGGAGGTAGC TCAGCTGCTG
    CTGGGGCTGG GAGCCGCCCA AGGACTACGG GACCAGGCCG GCTTAACACC CGGAGACGTC
    GCCCGTCAGC GCAACCACTG GGATCTGCTG ACGCTGCTGG AAGGGGCGGG GCCACCGGAG
    GCGCGTCACA AACCCACGCC GGGCCGCGGC GCCGGCACCT TCCAGCGCGC GCGGACCGCG
    TCCGGAAGCG TGCCCCCGCG TGGGGGCGGG GCTGTGCCGC GCTGCCGGAC GCTGTCAGCC
    GGAGCCCGTC CGCAAGCCCG GACTTTGTCC GTAGACTTGG CCGCGCACGG GGGCGGGGCC
    TACTCGCACT GCCGGAGCCT ATCGAAAGGA ACAGGAGAAG GCCCGCCCCT CCGCGGCCGT
    AGGTTCTCAG CAGGCATGCG CGGGCCTCGG CCCAACCCTG CAATGGTGCG AGGAAGACCC
    GGGGTCTCGG CCGGGAGCGG GGGCGTGGCT GCAACTGTTG ACTGGCCCTG CGACTGGGTG
    CCCCTGGGAG CCTGCGGCCC CGCTTCCAAC ACGCCAATCC CGCCTCCTTG CCTAACACCG
    TCCCCTGAGC GGGGATCCCC TCAAGTTGCC TGGGGTCCCC CAGCCCACCA AGTAATACCC
    TTAAATGCAG GAGGCGAGGG TCAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193877.1
    CDS1..5970
    Translation

    Target ORF information:

    RefSeq Version NM_001206948.1
    Organism Bos taurus(cattle)
    Definition Bos taurus notch 4 (NOTCH4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206948.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCAGCCCC CTTCACTGCT GCTGCCACTG CTGCTGCTGC TGTGTCAGCC AGTCGTCAGG 
    ACCAGAGGGC TGCAGTGCGG GAGTTTCCCA GAACCCTGTG CCAACGGAGG CACCTGCCTG
    AGCCTATCCC AGGGGCAAGG GACCTGCCAG TGTGCCCCTG GCTTCCTGGG TGAGACATGC
    CAGTTTCCTG ACCCCTGCCA GGATGCCCAG CCCTGCCAGA ACGGAGGCAG CTGCCACACC
    TTTCTTCCCA CCCTTCCCGG CTCCCCTGGC ACCCCCTCTC CCATGGCCCC CAGCTTCTTC
    TGCACCTGCC CCTCTGGCTT CACTGGCGAT AGGTGCCAGG CCCAGATCAG GGACCCCTGT
    TCCTCCTTCT GTTCCAAAAT GGGTCGCTGC CACCTCCAGG ACTCGGGCCG CCCACGATGC
    TCCTGCATGC CAGGATGGAC GGGTGAGCAC TGCCAGCTTC GAGACTTCTG CTCAGCCAAC
    CCCTGCGTCA ATGGAGGAGT GTGTCTGGCC ACCTACCCCC AGATCCAGTG CCGCTGCCCA
    CCCGGCTTCG AGGGCCACGC CTGCGAACAC GACGTCAACG AGTGTTACCT GGACCCGGGA
    CCGTGCCCCA AGGGCACCAC CTGCCATAAC ACCCTGGGAT CCTGCCAGTG TCTCTGCCCT
    GCTGGGCGGG AGGGTCCACG CTGTGGACTC CGGCCGGGAC CCTGCACCCC CAGGGGCTGT
    CTCAATGGGG GCACCTGTCA ACTGGTTCCA GGGAGAGACT CCACTTTCCA CCTGTGCCTC
    TGTCCCCCAG GCTTCACAGG CCCAAGCTGT GAGGTGAATC CAGATGACTG TGCTGGGCAC
    CAGTGTCAGA ACGGGGGCAC CTGCCAGGAT GGGCTGGGCA CCTACACCTG CCTCTGCCCA
    GAGGCCTGGA CAGGCTGGGA TTGCTCTGAA GATGTGGACG AATGTGAGGT TCAGGGTCCC
    CCTCGCTGCA GAAATGGGGG TACCTGCCAG AACTCAGCTG GAGACTTCTA CTGTGTGTGT
    GTGAGTGGCT GGGGAGGCGC CGGCTGCGAA GAGAACCTGG ACGACTGTGT GGCCGCCACC
    TGTGCCCCCG GATCCACCTG CATTGACCGC GTGGGCTCCT TCTCCTGCCT CTGCCCGCCT
    GGGCGCACAG GCCTCCTGTG CCACATGGAG GACATGTGTC TGAGTCAGCC ATGCCATGAG
    GAAGCCCAGT GCAGCACCAA CCCCCTGAGT GGCTCCACAC TCTGCGTATG TCAGCCAGGC
    TACACAGGGC CCACCTGCCA CCAGGACCTG GACGAGTGTC AGATGGCTCA GCAAGGCCCC
    AGTCCCTGTG AACACGGCGG CTCCTGCCTC AACACGCCCG GCTCCTTCGA GTGCCTCTGT
    CCGCCTGGCT ACACGGGTTC CCGCTGCGAG GCCGATCACA ACGAGTGCCT GTCGCAGCCC
    TGCCACAGGG GCGGCACCTG CCTGGACCTG CTCGCCACCT TCCAGTGCCT CTGCCCACCA
    GGCTTAGAAG GGCAGCTCTG TGAGGTGGAG ATAGACGAGT GCGCCTCGGC CCCCTGCTTG
    AACCAGGCTG ACTGCCACGA CCTGCTCAAC GGCTTCCAGT GCATCTGCCA GCCAGGTTTC
    ACTGGCCCCC GGTGTGAGGA GGACATCAAC GAATGTCAAA GCTCTCCCTG TGCCAATGGC
    GGGGAGTGCC AGGACCAGCC TGGATCCTTC CATTGCAAGT GTCCCCCAGG CTTTGAAGGC
    CCACGCTGTC AGGAGGAGGT GGACGAGTGT CTGAGTGGCC CATGCCCCAC CGGAGCCAGC
    TGCCTTGACC TCCCAGGAGC TTTCCTTTGC GTCTGCCCCT CTGGCTTCAC AGGCCATCTC
    TGTGAAATTC CCCTGTGTGC CCCCAACCTG TGCCAGCCCA AACAAAAATG TCAGGATCAG
    GAGGACAATG CCCACTGCCT CTGCCCTGAT GGAAACCCAG GCTGTGTCCC CACAGAAGAC
    AACTGCACCT GCCACCATGG GCACTGCCAG AGATCTTCAT GTGTGTGTGA TGGGGGCTGG
    ACAGGACCAG AGTGTGACAC AGATCTGGGG GGCTGTGTCT CCACCCCCTG TGCCCACGGA
    GGGACCTGCC ACCCCCAGCC CTTTGGCTAC AACTGCACCT GCCCCACAGG TTACACAGGG
    CCCACTTGCA GTGAGGAGGT GACAGCCTGT CACTCAGGGC CCTGTCTCAA TGGTGGTTCC
    TGCAGCCCCA GCCCCGGGGG CTACACCTGC ACCTGCCCTC TGAGCCACAC GGGGCTCCGC
    TGCCAGACCA GCATTGACCA TTGTGCCTCT GCACTGTGCC TCAATGGGGG CACCTGTGTG
    AACAAGCCGG GCACCTTCTC CTGCCTCTGC ACCCCAGGCT TCCAGGGCCC ACGCTGCGAG
    GGAAGGACCC GTCCCAGCTG CGCAGACAGC CCCTGTAGGA ACATGGCAAC CTGCCAAGAC
    AGCCCTCAGG GTCCCCGCTG CCTCTGTCCC CCTGGCTACA CAGGAGGCAG CTGTCAGACG
    CTGATGGACT TATGCGCCCA GAAGCCCTGC CCACAAAATT CCCACTGCCT CCAGACTGGG
    CCCTCCTTCC AGTGCCTGTG CCTCCAGGGA TGGACTGGGC CTCTCTGCAA CCTGCCGCTG
    TCCTCCTGCC AGAAGGTTGC TCTGAGCCAA GGCACAGAAG TCTCTTCCCT GTGCCAGAAC
    GGAGGCGTCT GCATTGACAG CGGCCCCTCC CATTTCTGCC ACTGTCCCCC TGGATTCCAA
    GGCAGCATAT GCCAGGACCA GGTGAACCCC TGTGAGTCCA GGCCCTGCCA GCATGGGGCC
    ACCTGCGTGG CCCAACCCAA CGGTTATCTC TGCCAGTGTG CACCCGGCTA CAGTGGACAG
    AACTGCTCAG AGGAACCGGA CGCATGTCAA TCCCAGCCCT GTCACAACCA GGGGACCTGT
    ATCTCCAAAC CCGGAGGCTT CCACTGTGCC TGCCCGCCAG GCTTTGTGGG GCTGCGCTGT
    GAGGGGGACG TGGATGAGTG TCTGGACCGG CCCTGTCACC CCACAGGCAC TGCAGCCTGC
    CACTCTCTGG CCAATGCCTT CTACTGCCAG TGTCTGCCTG GACACACAGG CCAGTGGTGT
    GAAGTGGAGC TAGACCCCTG CCAGAGCCAG CCCTGTGCCC ACAGCGGGTC ATGTGAGGCC
    ACAGCAGGGC CACCCCCAGG ATTCACCTGC CACTGTCCCC AGGGTTTTGA AGGCCCCACC
    TGCAGCCACA GAGCCCCCTC CTGTGGTCTC CATCACTGCC ACCATGGTGG CCTGTGTCTG
    CCCTCCCCCA AGCCTGGACT CCCACCCCGC TGCGCCTGCC TCAATGGTTA TGGGGGTCCG
    GATTGCCTGA CCCCACCAGC TCCTTCAGGC TGTGGCCCTC CTTCTCCATG CCTACACAAT
    GGCAGCTGCT CAGAGACCCC CGGGCTGGGG GGCCCAGGCT TTCGCTGTTC CTGCCCTGCC
    AGCTCTCCAG GGCCCCGGTG TCAGAGGCCC GGAGTCAAGG GATGCGAGGG CAGAAGTGGA
    GATGGGGCCT GTGATGCTGG CTGCAGTGGC CCAGGAGGAA ACTGGGATGG GGGGGACTGC
    TCCCTGGGGG TCCCAGACCC CTGGAAGGGC TGCCCCTCCC ACTCCCGGTG CTGGCTTCTT
    TTCCGGGACG GGCAGTGCCA CCCACAGTGT GACTCTGAAG AGTGTTTGTT TGATGGCTAC
    GACTGTGAGA CTCCTCCAGC CTGCATTCCA GCCTATGACC AGTACTGCCG CGATCACTTC
    CACAATGGAC ACTGCGAGAA GGGCTGCAAC ACGGCAGAAT GTGGCTGGGA CGGGGGAGAC
    TGCAGGCCGG AAGATGGAAA TTCAGAGTGG GGGCCCTCCC TGGCCTTGTT GGTGGTGCTG
    AGCCCCTCAA CCCTGGACCA GCAGCTGCTC GCCCTGGCCC GGGTGCTGTC CCTGACTCTG
    AGGGTAGGGC TCTGGGTGAG GAAGGACAGT GAAGGCAAGG ACATGGTGTA CCCCTATCCT
    GGAGCCCAAG CAGAAGAGGA GCTAGGAGGA ACCCCAGACC CCTCTCAGCA GGAGAGAGCA
    GCCCCCCAAA CAGAGCCTAC AGGCAAGGAG ACAGATTCCC TGAGCACTGG GTTTGTGGTG
    GTGATGGGTG TGGACTTGTC CCACTGTGGC CCCGAGCGCC CTGCATCCCG CTGCCCCTGG
    GACCCTGGGC TCCTACTCCG CTTCCTTGCT GCAATGGCTG CAGTGGGGGC CCTGGAACCC
    CTACTGCCAG GACCCCTGCT GGCTGCCCAC CCTCGTGCAG GCACCAAGCC CTCCGCCAAC
    CAGCTTCCCT GGCCTGTACT GTGCTCCCCA GTGGTCGGGG TCCTTCTCCT AGCCCTCGGG
    GCTCTTCTCG TCCTCCAGCT CATCCGTCGC AAGCGCCGAG AGCATGGGGC CCTCTGGCTG
    CCCCCTGGGT TCACTCGCAG GCCCCGGACT CAGCCAGTTT CCCGCCGACG TCGACCCCCG
    CTGGGCGAGG ACAGCATCGG CCTCAAGGCA CTGAAAGCAG AGGCAGACAT GGATGAGGAT
    GGTGTTGTGA TGTGCTCAGT CCCCAAGGAA GGAGAGGAGG CTGAAGAAAT GGCCTCACCC
    CCCAAGTGCC AGCTCTGGTC TCTGAGCAGT GACTGCCAAG AGCTCCCCCA GGCAGCCATG
    CTGACTCCTC CCCAGGATTC TGAGATGGAT GTCCCTGACG TGGACACCCA GGGACCTGAT
    GGGGTGACAC CCCTGATGTC AGCAGTCTGC TGTGGGGGCG TGGAGTCCAG GACCTACCAG
    GAGACATGGC TGGGAAGTCC TGAGCCCTGG GAACCTCTGC TGGAGGGAGG GGCCTGTCCC
    CAGGTTCACA CTGTGGGCAC TGGGGAGACA CCCCTGCACC TGGCTGCTCG ATTCTCCCGG
    CCAACCGCTG CCCGCCGCCT CCTCGAGGCT GGAGCAAACC CCAACCAGCC AGACCGGGCA
    GGGCGCACCC CGCTTCACAC GGCTGTGGCT GCTGATGCTA GGGAGGTTTG CCAGCTCTTA
    CTCCGCAGCA GACAGACTGC AGTGGATGCA CGGACAGAGG ATGGGACCAC AGCCCTGATG
    CTGGCCGCCA GGCTGGCTGT CGAAGACCTG GTTGAAGAAC TGATTGCAGC CCAAGCAGAT
    GTGGGGGCCA GAGACAAATG GGGAAAAACA GCGCTGCACT GGGCCGCCGC CGTGAACAAC
    GCCCGGGCCG CCCGCTCCCT TCTCCAGGCG GGAGCCGATA AAGACGCACA GGATGGCAGG
    GAGCAGACGC CGCTGTTCCT GGCGGCCCGA GAAGGGGCGA TGGAGGTAGC TCAGCTGCTG
    CTGGGGCTGG GAGCCGCCCA AGGACTACGG GACCAGGCCG GCTTAACACC CGGAGACGTC
    GCCCGTCAGC GCAACCACTG GGATCTGCTG ACGCTGCTGG AAGGGGCGGG GCCACCGGAG
    GCGCGTCACA AACCCACGCC GGGCCGCGGC GCCGGCACCT TCCAGCGCGC GCGGACCGCG
    TCCGGAAGCG TGCCCCCGCG TGGGGGCGGG GCTGTGCCGC GCTGCCGGAC GCTGTCAGCC
    GGAGCCCGTC CGCAAGCCCG GACTTTGTCC GTAGACTTGG CCGCGCACGG GGGCGGGGCC
    TACTCGCACT GCCGGAGCCT ATCGAAAGGA ACAGGAGAAG GCCCGCCCCT CCGCGGCCGT
    AGGTTCTCAG CAGGCATGCG CGGGCCTCGG CCCAACCCTG CAATGGTGCG AGGAAGACCC
    GGGGTCTCGG CCGGGAGCGG GGGCGTGGCT GCAACTGTTG ACTGGCCCTG CGACTGGGTG
    CCCCTGGGAG CCTGCGGCCC CGCTTCCAAC ACGCCAATCC CGCCTCCTTG CCTAACACCG
    TCCCCTGAGC GGGGATCCCC TCAAGTTGCC TGGGGTCCCC CAGCCCACCA AGTAATACCC
    TTAAATGCAG GAGGCGAGGG TCAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32160
    Clone ID Related Accession (Same CDS sequence) XM_010818346.2 , XM_005223669.3
    Accession Version XM_010818346.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurogenic locus notch homolog protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCAGCCCC CTTCACTGCT GCTGCCACTG CTGCTGCTGC TGTGTCAGCC AGTCGTCAGG 
    ACCAGAGGGC TGCAGTGCGG GAGTTTCCCA GAACCCTGTG CCAACGGAGG CACCTGCCTG
    AGCCTATCCC AGGGGCAAGG GACCTGCCAG TGTGCCCCTG GCTTCCTGGG TGAGACATGC
    CAGTTTCCTG ACCCCTGCCA GGATGCCCAG CCCTGCCAGA ACGGAGGCAG CTGCCACACC
    TTTCTTCCCA CCCTTCCCGG CTCCCCTGGC ACCCCCTCTC CCATGGCCCC CAGCTTCTTC
    TGCACCTGCC CCTCTGGCTT CACTGGCGAT AGGTGCCAGG CCCAGATCAG GGACCCCTGT
    TCCTCCTTCT GTTCCAAAAT GGGTCGCTGC CACCTCCAGG ACTCGGGCCG CCCACGATGC
    TCCTGCATGC CAGGATGGAC GGGTGAGCAC TGCCAGCTTC GAGACTTCTG CTCAGCCAAC
    CCCTGCGTCA ATGGAGGAGT GTGTCTGGCC ACCTACCCCC AGATCCAGTG CCGCTGCCCA
    CCCGGCTTCG AGGGCCACGC CTGCGAACAC GACGTCAACG AGTGTTACCT GGACCCGGGA
    CCGTGCCCCA AGGGCACCAC CTGCCATAAC ACCCTGGGAT CCTGCCAGTG TCTCTGCCCT
    GCTGGGCGGG AGGGTCCACG CTGTGGACTC CGGCCGGGAC CCTGCACCCC CAGGGGCTGT
    CTCAATGGGG GCACCTGTCA ACTGGTTCCA GGGAGAGACT CCACTTTCCA CCTGTGCCTC
    TGTCCCCCAG GCTTCACAGG CCCAAGCTGT GAGGTGAATC CAGATGACTG TGCTGGGCAC
    CAGTGTCAGA ACGGGGGCAC CTGCCAGGAT GGGCTGGGCA CCTACACCTG CCTCTGCCCA
    GAGGCCTGGA CAGGCTGGGA TTGCTCTGAA GATGTGGACG AATGTGAGGT TCAGGGTCCC
    CCTCGCTGCA GAAATGGGGG TACCTGCCAG AACTCAGCTG GAGACTTCTA CTGTGTGTGT
    GTGAGTGGCT GGGGAGGCGC CGGCTGCGAA GAGAACCTGG ACGACTGTGT GGCCGCCACC
    TGTGCCCCCG GATCCACCTG CATTGACCGC GTGGGCTCCT TCTCCTGCCT CTGCCCGCCT
    GGGCGCACAG GCCTCCTGTG CCACATGGAG GACATGTGTC TGAGTCAGCC ATGCCATGAG
    GAAGCCCAGT GCAGCACCAA CCCCCTGAGT GGCTCCACAC TCTGCGTATG TCAGCCAGGC
    TACACAGGGC CCACCTGCCA CCAGGACCTG GACGAGTGTC AGATGGCTCA GCAAGGCCCC
    AGTCCCTGTG AACACGGCGG CTCCTGCCTC AACACGCCCG GCTCCTTCGA GTGCCTCTGT
    CCGCCTGGCT ACACGGGTTC CCGCTGCGAG GCCGATCACA ACGAGTGCCT GTCGCAGCCC
    TGCCACAGGG GCGGCACCTG CCTGGACCTG CTCGCCACCT TCCAGTGCCT CTGCCCACCA
    GGCTTAGAAG GGCAGCTCTG TGAGGTGGAG ATAGACGAGT GCGCCTCGGC CCCCTGCTTG
    AACCAGGCTG ACTGCCACGA CCTGCTCAAC GGCTTCCAGT GCATCTGCCA GCCAGGTTTC
    ACTGGCCCCC GGTGTGAGGA GGACATCAAC GAATGTCAAA GCTCTCCCTG TGCCAATGGC
    GGGGAGTGCC AGGACCAGCC TGGATCCTTC CATTGCAAGT GTCCCCCAGG CTTTGAAGGC
    CCACGCTGTC AGGAGGAGGT GGACGAGTGT CTGAGTGGCC CATGCCCCAC CGGAGCCAGC
    TGCCTTGACC TCCCAGGAGC TTTCCTTTGC GTCTGCCCCT CTGGCTTCAC AGGCCATCTC
    TGTGAAATTC CCCTGTGTGC CCCCAACCTG TGCCAGCCCA AACAAAAATG TCAGGATCAG
    GAGGACAATG CCCACTGCCT CTGCCCTGAT GGAAACCCAG GCTGTGTCCC CACAGAAGAC
    AACTGCACCT GCCACCATGG GCACTGCCAG AGATCTTCAT GTGTGTGTGA TGGGGGCTGG
    ACAGGACCAG AGTGTGACAC AGATCTGGGG GGCTGTGTCT CCACCCCCTG TGCCCACGGA
    GGGACCTGCC ACCCCCAGCC CTTTGGCTAC AACTGCACCT GCCCCACAGG TTACACAGGG
    CCCACTTGCA GTGAGGAGGT GACAGCCTGT CACTCAGGGC CCTGTCTCAA TGGTGGTTCC
    TGCAGCCCCA GCCCCGGGGG CTACACCTGC ACCTGCCCTC TGAGCCACAC GGGGCTCCGC
    TGCCAGACCA GCATTGACCA TTGTGCCTCT GCACTGTGCC TCAATGGGGG CACCTGTGTG
    AACAAGCCGG GCACCTTCTC CTGCCTCTGC ACCCCAGGCT TCCAGGGCCC ACGCTGCGAG
    GGAAGGACCC GTCCCAGCTG CGCAGACAGC CCCTGTAGGA ACATGGCAAC CTGCCAAGAC
    AGCCCTCAGG GTCCCCGCTG CCTCTGTCCC CCTGGCTACA CAGGAGGCAG CTGTCAGACG
    CTGATGGACT TATGCGCCCA GAAGCCCTGC CCACAAAATT CCCACTGCCT CCAGACTGGG
    CCCTCCTTCC AGTGCCTGTG CCTCCAGGGA TGGACTGGGC CTCTCTGCAA CCTGCCGCTG
    TCCTCCTGCC AGAAGGTTGC TCTGAGCCAA GGCACAGAAG TCTCTTCCCT GTGCCAGAAC
    GGAGGCGTCT GCATTGACAG CGGCCCCTCC CATTTCTGCC ACTGTCCCCC TGGATTCCAA
    GGCAGCATAT GCCAGGACCA GGTGAACCCC TGTGAGTCCA GGCCCTGCCA GCATGGGGCC
    ACCTGCGTGG CCCAACCCAA CGGTTATCTC TGCCAGTGTG CACCCGGCTA CAGTGGACAG
    AACTGCTCAG AGGAACCGGA CGCATGTCAA TCCCAGCCCT GTCACAACCA GGGGACCTGT
    ATCTCCAAAC CCGGAGGCTT CCACTGTGCC TGCCCGCCAG GCTTTGTGGG GCTGCGCTGT
    GAGGGGGACG TGGATGAGTG TCTGGACCGG CCCTGTCACC CCACAGGCAC TGCAGCCTGC
    CACTCTCTGG CCAATGCCTT CTACTGCCAG TGTCTGCCTG GACACACAGG CCAGTGGTGT
    GAAGTGGAGC TAGACCCCTG CCAGAGCCAG CCCTGTGCCC ACAGCGGGTC ATGTGAGGCC
    ACAGCAGGGC CACCCCCAGG ATTCACCTGC CACTGTCCCC AGGGTTTTGA AGGCCCCACC
    TGCAGCCACA GAGCCCCCTC CTGTGGTCTC CATCACTGCC ACCATGGTGG CCTGTGTCTG
    CCCTCCCCCA AGCCTGGACT CCCACCCCGC TGCGCCTGCC TCAATGGTTA TGGGGGTCCG
    GATTGCCTGA CCCCACCAGC TCCTTCAGGC TGTGGCCCTC CTTCTCCATG CCTACACAAT
    GGCAGCTGCT CAGAGACCCC CGGGCTGGGG GGCCCAGGCT TTCGCTGTTC CTGCCCTGCC
    AGCTCTCCAG GGCCCCGGTG TCAGAGGCCC GGAGTCAAGG GATGCGAGGG CAGAAGTGGA
    GATGGGGCCT GTGATGCTGG CTGCAGTGGC CCAGGAGGAA ACTGGGATGG GGGGGACTGC
    TCCCTGGGGG TCCCAGACCC CTGGAAGGGC TGCCCCTCCC ACTCCCGGTG CTGGCTTCTT
    TTCCGGGACG GGCAGTGCCA CCCACAGTGT GACTCTGAAG AGTGTTTGTT TGATGGCTAC
    GACTGTGAGA CTCCTCCAGC CTGCATTCCA GCCTATGACC AGTACTGCCG CGATCACTTC
    CACAATGGAC ACTGCGAGAA GGGCTGCAAC ACGGCAGAAT GTGGCTGGGA CGGGGGAGAC
    TGCAGGCCGG AAGATGGAAA TTCAGAGTGG GGGCCCTCCC TGGCCTTGTT GGTGGTGCTG
    AGCCCCTCAA CCCTGGACCA GCAGCTGCTC GCCCTGGCCC GGGTGCTGTC CCTGACTCTG
    AGGGTAGGGC TCTGGGTGAG GAAGGACAGT GAAGGCAAGG ACATGGTGTA CCCCTATCCT
    GGAGCCCAAG CAGAAGAGGA GCTAGGAGGA ACCCCAGACC CCTCTCAGCA GGAGAGAGCA
    GCCCCCCAAA CAGAGCCTAC AGGCAAGGAG ACAGATTCCC TGAGCACTGG GTTTGTGGTG
    GTGATGGGTG TGGACTTGTC CCACTGTGGC CCCGAGCGCC CTGCATCCCG CTGCCCCTGG
    GACCCTGGGC TCCTACTCCG CTTCCTTGCT GCAATGGCTG CAGTGGGGGC CCTGGAACCC
    CTACTGCCAG GACCCCTGCT GGCTGCCCAC CCTCGTGCAG GCACCAAGCC CTCCGCCAAC
    CAGCTTCCCT GGCCTGTACT GTGCTCCCCA GTGGTCGGGG TCCTTCTCCT AGCCCTCGGG
    GCTCTTCTCG TCCTCCAGCT CATCCGTCGC AAGCGCCGAG AGCATGGGGC CCTCTGGCTG
    CCCCCTGGGT TCACTCGCAG GCCCCGGACT CAGCCAGTTT CCCGCCGACG TCGACCCCCG
    CTGGGCGAGG ACAGCATCGG CCTCAAGGCA CTGAAAGCAG AGGCAGACAT GGATGAGGAT
    GGTGTTGTGA TGTGCTCAGT CCCCAAGGAA GGAGAGGAGG TGGGCCAGGC TGAAGAAATG
    GCCTCACCCC CCAAGTGCCA GCTCTGGTCT CTGAGCAGTG ACTGCCAAGA GCTCCCCCAG
    GCAGCCATGC TGACTCCTCC CCAGGATTCT GAGATGGATG TCCCTGACGT GGACACCCAG
    GGACCTGATG GGGTGACACC CCTGATGTCA GCAGTCTGCT GTGGGGGCGT GGAGTCCAGG
    ACCTACCAGG AGACATGGCT GGGAAGTCCT GAGCCCTGGG AACCTCTGCT GGAGGGAGGG
    GCCTGTCCCC AGGTTCACAC TGTGGGCACT GGGGAGACAC CCCTGCACCT GGCTGCTCGA
    TTCTCCCGGC CAACCGCTGC CCGCCGCCTC CTCGAGGCTG GAGCAAACCC CAACCAGCCA
    GACCGGGCAG GGCGCACCCC GCTTCACACG GCTGTGGCTG CTGATGCTAG GGAGGTTTGC
    CAGCTCTTAC TCCGCAGCAG ACAGACTGCA GTGGATGCAC GGACAGAGGA TGGGACCACA
    GCCCTGATGC TGGCCGCCAG GCTGGCTGTC GAAGACCTGG TTGAAGAACT GATTGCAGCC
    CAAGCAGATG TGGGGGCCAG AGACAAATGG GGAAAAACAG CGCTGCACTG GGCCGCCGCC
    GTGAACAACG CCCGGGCCGC CCGCTCCCTT CTCCAGGCGG GAGCCGATAA AGACGCACAG
    GATGGCAGGG AGCAGACGCC GCTGTTCCTG GCGGCCCGAG AAGGGGCGAT GGAGGTAGCT
    CAGCTGCTGC TGGGGCTGGG AGCCGCCCAA GGACTACGGG ACCAGGCCGG CTTAACACCC
    GGAGACGTCG CCCGTCAGCG CAACCACTGG GATCTGCTGA CGCTGCTGGA AGGGGCGGGG
    CCACCGGAGG CGCGTCACAA ACCCACGCCG GGCCGCGGCG CCGGCACCTT CCAGCGCGCG
    CGGACCGCGT CCGGAAGCGT GCCCCCGCGT GGGGGCGGGG CTGTGCCGCG CTGCCGGACG
    CTGTCAGCCG GAGCCCGTCC GCAAGCCCGG ACTTTGTCCG TAGACTTGGC CGCGCACGGG
    GGCGGGGCCT ACTCGCACTG CCGGAGCCTA TCGAAAGGAA CAGGAGAAGG CCCGCCCCTC
    CGCGGCCGTA GGTTCTCAGC AGGCATGCGC GGGCCTCGGC CCAACCCTGC AATGGTGCGA
    GGAAGACCCG GGGTCTCGGC CGGGAGCGGG GGCGTGGCTG CAACTGTTGA CTGGCCCTGC
    GACTGGGTGC CCCTGGGAGC CTGCGGCCCC GCTTCCAACA CGCCAATCCC GCCTCCTTGC
    CTAACACCGT CCCCTGAGCG GGGATCCCCT CAAGTTGCCT GGGGTCCCCC AGCCCACCAA
    GTAATACCCT TAAATGCAGG AGGCGAGGGT CAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816648.1
    CDS128..6106
    Translation

    Target ORF information:

    RefSeq Version XM_010818346.2
    Organism Bos taurus(cattle)
    Definition Bos taurus notch 4 (NOTCH4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818346.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCAGCCCC CTTCACTGCT GCTGCCACTG CTGCTGCTGC TGTGTCAGCC AGTCGTCAGG 
    ACCAGAGGGC TGCAGTGCGG GAGTTTCCCA GAACCCTGTG CCAACGGAGG CACCTGCCTG
    AGCCTATCCC AGGGGCAAGG GACCTGCCAG TGTGCCCCTG GCTTCCTGGG TGAGACATGC
    CAGTTTCCTG ACCCCTGCCA GGATGCCCAG CCCTGCCAGA ACGGAGGCAG CTGCCACACC
    TTTCTTCCCA CCCTTCCCGG CTCCCCTGGC ACCCCCTCTC CCATGGCCCC CAGCTTCTTC
    TGCACCTGCC CCTCTGGCTT CACTGGCGAT AGGTGCCAGG CCCAGATCAG GGACCCCTGT
    TCCTCCTTCT GTTCCAAAAT GGGTCGCTGC CACCTCCAGG ACTCGGGCCG CCCACGATGC
    TCCTGCATGC CAGGATGGAC GGGTGAGCAC TGCCAGCTTC GAGACTTCTG CTCAGCCAAC
    CCCTGCGTCA ATGGAGGAGT GTGTCTGGCC ACCTACCCCC AGATCCAGTG CCGCTGCCCA
    CCCGGCTTCG AGGGCCACGC CTGCGAACAC GACGTCAACG AGTGTTACCT GGACCCGGGA
    CCGTGCCCCA AGGGCACCAC CTGCCATAAC ACCCTGGGAT CCTGCCAGTG TCTCTGCCCT
    GCTGGGCGGG AGGGTCCACG CTGTGGACTC CGGCCGGGAC CCTGCACCCC CAGGGGCTGT
    CTCAATGGGG GCACCTGTCA ACTGGTTCCA GGGAGAGACT CCACTTTCCA CCTGTGCCTC
    TGTCCCCCAG GCTTCACAGG CCCAAGCTGT GAGGTGAATC CAGATGACTG TGCTGGGCAC
    CAGTGTCAGA ACGGGGGCAC CTGCCAGGAT GGGCTGGGCA CCTACACCTG CCTCTGCCCA
    GAGGCCTGGA CAGGCTGGGA TTGCTCTGAA GATGTGGACG AATGTGAGGT TCAGGGTCCC
    CCTCGCTGCA GAAATGGGGG TACCTGCCAG AACTCAGCTG GAGACTTCTA CTGTGTGTGT
    GTGAGTGGCT GGGGAGGCGC CGGCTGCGAA GAGAACCTGG ACGACTGTGT GGCCGCCACC
    TGTGCCCCCG GATCCACCTG CATTGACCGC GTGGGCTCCT TCTCCTGCCT CTGCCCGCCT
    GGGCGCACAG GCCTCCTGTG CCACATGGAG GACATGTGTC TGAGTCAGCC ATGCCATGAG
    GAAGCCCAGT GCAGCACCAA CCCCCTGAGT GGCTCCACAC TCTGCGTATG TCAGCCAGGC
    TACACAGGGC CCACCTGCCA CCAGGACCTG GACGAGTGTC AGATGGCTCA GCAAGGCCCC
    AGTCCCTGTG AACACGGCGG CTCCTGCCTC AACACGCCCG GCTCCTTCGA GTGCCTCTGT
    CCGCCTGGCT ACACGGGTTC CCGCTGCGAG GCCGATCACA ACGAGTGCCT GTCGCAGCCC
    TGCCACAGGG GCGGCACCTG CCTGGACCTG CTCGCCACCT TCCAGTGCCT CTGCCCACCA
    GGCTTAGAAG GGCAGCTCTG TGAGGTGGAG ATAGACGAGT GCGCCTCGGC CCCCTGCTTG
    AACCAGGCTG ACTGCCACGA CCTGCTCAAC GGCTTCCAGT GCATCTGCCA GCCAGGTTTC
    ACTGGCCCCC GGTGTGAGGA GGACATCAAC GAATGTCAAA GCTCTCCCTG TGCCAATGGC
    GGGGAGTGCC AGGACCAGCC TGGATCCTTC CATTGCAAGT GTCCCCCAGG CTTTGAAGGC
    CCACGCTGTC AGGAGGAGGT GGACGAGTGT CTGAGTGGCC CATGCCCCAC CGGAGCCAGC
    TGCCTTGACC TCCCAGGAGC TTTCCTTTGC GTCTGCCCCT CTGGCTTCAC AGGCCATCTC
    TGTGAAATTC CCCTGTGTGC CCCCAACCTG TGCCAGCCCA AACAAAAATG TCAGGATCAG
    GAGGACAATG CCCACTGCCT CTGCCCTGAT GGAAACCCAG GCTGTGTCCC CACAGAAGAC
    AACTGCACCT GCCACCATGG GCACTGCCAG AGATCTTCAT GTGTGTGTGA TGGGGGCTGG
    ACAGGACCAG AGTGTGACAC AGATCTGGGG GGCTGTGTCT CCACCCCCTG TGCCCACGGA
    GGGACCTGCC ACCCCCAGCC CTTTGGCTAC AACTGCACCT GCCCCACAGG TTACACAGGG
    CCCACTTGCA GTGAGGAGGT GACAGCCTGT CACTCAGGGC CCTGTCTCAA TGGTGGTTCC
    TGCAGCCCCA GCCCCGGGGG CTACACCTGC ACCTGCCCTC TGAGCCACAC GGGGCTCCGC
    TGCCAGACCA GCATTGACCA TTGTGCCTCT GCACTGTGCC TCAATGGGGG CACCTGTGTG
    AACAAGCCGG GCACCTTCTC CTGCCTCTGC ACCCCAGGCT TCCAGGGCCC ACGCTGCGAG
    GGAAGGACCC GTCCCAGCTG CGCAGACAGC CCCTGTAGGA ACATGGCAAC CTGCCAAGAC
    AGCCCTCAGG GTCCCCGCTG CCTCTGTCCC CCTGGCTACA CAGGAGGCAG CTGTCAGACG
    CTGATGGACT TATGCGCCCA GAAGCCCTGC CCACAAAATT CCCACTGCCT CCAGACTGGG
    CCCTCCTTCC AGTGCCTGTG CCTCCAGGGA TGGACTGGGC CTCTCTGCAA CCTGCCGCTG
    TCCTCCTGCC AGAAGGTTGC TCTGAGCCAA GGCACAGAAG TCTCTTCCCT GTGCCAGAAC
    GGAGGCGTCT GCATTGACAG CGGCCCCTCC CATTTCTGCC ACTGTCCCCC TGGATTCCAA
    GGCAGCATAT GCCAGGACCA GGTGAACCCC TGTGAGTCCA GGCCCTGCCA GCATGGGGCC
    ACCTGCGTGG CCCAACCCAA CGGTTATCTC TGCCAGTGTG CACCCGGCTA CAGTGGACAG
    AACTGCTCAG AGGAACCGGA CGCATGTCAA TCCCAGCCCT GTCACAACCA GGGGACCTGT
    ATCTCCAAAC CCGGAGGCTT CCACTGTGCC TGCCCGCCAG GCTTTGTGGG GCTGCGCTGT
    GAGGGGGACG TGGATGAGTG TCTGGACCGG CCCTGTCACC CCACAGGCAC TGCAGCCTGC
    CACTCTCTGG CCAATGCCTT CTACTGCCAG TGTCTGCCTG GACACACAGG CCAGTGGTGT
    GAAGTGGAGC TAGACCCCTG CCAGAGCCAG CCCTGTGCCC ACAGCGGGTC ATGTGAGGCC
    ACAGCAGGGC CACCCCCAGG ATTCACCTGC CACTGTCCCC AGGGTTTTGA AGGCCCCACC
    TGCAGCCACA GAGCCCCCTC CTGTGGTCTC CATCACTGCC ACCATGGTGG CCTGTGTCTG
    CCCTCCCCCA AGCCTGGACT CCCACCCCGC TGCGCCTGCC TCAATGGTTA TGGGGGTCCG
    GATTGCCTGA CCCCACCAGC TCCTTCAGGC TGTGGCCCTC CTTCTCCATG CCTACACAAT
    GGCAGCTGCT CAGAGACCCC CGGGCTGGGG GGCCCAGGCT TTCGCTGTTC CTGCCCTGCC
    AGCTCTCCAG GGCCCCGGTG TCAGAGGCCC GGAGTCAAGG GATGCGAGGG CAGAAGTGGA
    GATGGGGCCT GTGATGCTGG CTGCAGTGGC CCAGGAGGAA ACTGGGATGG GGGGGACTGC
    TCCCTGGGGG TCCCAGACCC CTGGAAGGGC TGCCCCTCCC ACTCCCGGTG CTGGCTTCTT
    TTCCGGGACG GGCAGTGCCA CCCACAGTGT GACTCTGAAG AGTGTTTGTT TGATGGCTAC
    GACTGTGAGA CTCCTCCAGC CTGCATTCCA GCCTATGACC AGTACTGCCG CGATCACTTC
    CACAATGGAC ACTGCGAGAA GGGCTGCAAC ACGGCAGAAT GTGGCTGGGA CGGGGGAGAC
    TGCAGGCCGG AAGATGGAAA TTCAGAGTGG GGGCCCTCCC TGGCCTTGTT GGTGGTGCTG
    AGCCCCTCAA CCCTGGACCA GCAGCTGCTC GCCCTGGCCC GGGTGCTGTC CCTGACTCTG
    AGGGTAGGGC TCTGGGTGAG GAAGGACAGT GAAGGCAAGG ACATGGTGTA CCCCTATCCT
    GGAGCCCAAG CAGAAGAGGA GCTAGGAGGA ACCCCAGACC CCTCTCAGCA GGAGAGAGCA
    GCCCCCCAAA CAGAGCCTAC AGGCAAGGAG ACAGATTCCC TGAGCACTGG GTTTGTGGTG
    GTGATGGGTG TGGACTTGTC CCACTGTGGC CCCGAGCGCC CTGCATCCCG CTGCCCCTGG
    GACCCTGGGC TCCTACTCCG CTTCCTTGCT GCAATGGCTG CAGTGGGGGC CCTGGAACCC
    CTACTGCCAG GACCCCTGCT GGCTGCCCAC CCTCGTGCAG GCACCAAGCC CTCCGCCAAC
    CAGCTTCCCT GGCCTGTACT GTGCTCCCCA GTGGTCGGGG TCCTTCTCCT AGCCCTCGGG
    GCTCTTCTCG TCCTCCAGCT CATCCGTCGC AAGCGCCGAG AGCATGGGGC CCTCTGGCTG
    CCCCCTGGGT TCACTCGCAG GCCCCGGACT CAGCCAGTTT CCCGCCGACG TCGACCCCCG
    CTGGGCGAGG ACAGCATCGG CCTCAAGGCA CTGAAAGCAG AGGCAGACAT GGATGAGGAT
    GGTGTTGTGA TGTGCTCAGT CCCCAAGGAA GGAGAGGAGG TGGGCCAGGC TGAAGAAATG
    GCCTCACCCC CCAAGTGCCA GCTCTGGTCT CTGAGCAGTG ACTGCCAAGA GCTCCCCCAG
    GCAGCCATGC TGACTCCTCC CCAGGATTCT GAGATGGATG TCCCTGACGT GGACACCCAG
    GGACCTGATG GGGTGACACC CCTGATGTCA GCAGTCTGCT GTGGGGGCGT GGAGTCCAGG
    ACCTACCAGG AGACATGGCT GGGAAGTCCT GAGCCCTGGG AACCTCTGCT GGAGGGAGGG
    GCCTGTCCCC AGGTTCACAC TGTGGGCACT GGGGAGACAC CCCTGCACCT GGCTGCTCGA
    TTCTCCCGGC CAACCGCTGC CCGCCGCCTC CTCGAGGCTG GAGCAAACCC CAACCAGCCA
    GACCGGGCAG GGCGCACCCC GCTTCACACG GCTGTGGCTG CTGATGCTAG GGAGGTTTGC
    CAGCTCTTAC TCCGCAGCAG ACAGACTGCA GTGGATGCAC GGACAGAGGA TGGGACCACA
    GCCCTGATGC TGGCCGCCAG GCTGGCTGTC GAAGACCTGG TTGAAGAACT GATTGCAGCC
    CAAGCAGATG TGGGGGCCAG AGACAAATGG GGAAAAACAG CGCTGCACTG GGCCGCCGCC
    GTGAACAACG CCCGGGCCGC CCGCTCCCTT CTCCAGGCGG GAGCCGATAA AGACGCACAG
    GATGGCAGGG AGCAGACGCC GCTGTTCCTG GCGGCCCGAG AAGGGGCGAT GGAGGTAGCT
    CAGCTGCTGC TGGGGCTGGG AGCCGCCCAA GGACTACGGG ACCAGGCCGG CTTAACACCC
    GGAGACGTCG CCCGTCAGCG CAACCACTGG GATCTGCTGA CGCTGCTGGA AGGGGCGGGG
    CCACCGGAGG CGCGTCACAA ACCCACGCCG GGCCGCGGCG CCGGCACCTT CCAGCGCGCG
    CGGACCGCGT CCGGAAGCGT GCCCCCGCGT GGGGGCGGGG CTGTGCCGCG CTGCCGGACG
    CTGTCAGCCG GAGCCCGTCC GCAAGCCCGG ACTTTGTCCG TAGACTTGGC CGCGCACGGG
    GGCGGGGCCT ACTCGCACTG CCGGAGCCTA TCGAAAGGAA CAGGAGAAGG CCCGCCCCTC
    CGCGGCCGTA GGTTCTCAGC AGGCATGCGC GGGCCTCGGC CCAACCCTGC AATGGTGCGA
    GGAAGACCCG GGGTCTCGGC CGGGAGCGGG GGCGTGGCTG CAACTGTTGA CTGGCCCTGC
    GACTGGGTGC CCCTGGGAGC CTGCGGCCCC GCTTCCAACA CGCCAATCCC GCCTCCTTGC
    CTAACACCGT CCCCTGAGCG GGGATCCCCT CAAGTTGCCT GGGGTCCCCC AGCCCACCAA
    GTAATACCCT TAAATGCAGG AGGCGAGGGT CAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32160
    Clone ID Related Accession (Same CDS sequence) XM_010818346.2 , XM_005223669.3
    Accession Version XM_005223669.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurogenic locus notch homolog protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223669.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCAGCCCC CTTCACTGCT GCTGCCACTG CTGCTGCTGC TGTGTCAGCC AGTCGTCAGG 
    ACCAGAGGGC TGCAGTGCGG GAGTTTCCCA GAACCCTGTG CCAACGGAGG CACCTGCCTG
    AGCCTATCCC AGGGGCAAGG GACCTGCCAG TGTGCCCCTG GCTTCCTGGG TGAGACATGC
    CAGTTTCCTG ACCCCTGCCA GGATGCCCAG CCCTGCCAGA ACGGAGGCAG CTGCCACACC
    TTTCTTCCCA CCCTTCCCGG CTCCCCTGGC ACCCCCTCTC CCATGGCCCC CAGCTTCTTC
    TGCACCTGCC CCTCTGGCTT CACTGGCGAT AGGTGCCAGG CCCAGATCAG GGACCCCTGT
    TCCTCCTTCT GTTCCAAAAT GGGTCGCTGC CACCTCCAGG ACTCGGGCCG CCCACGATGC
    TCCTGCATGC CAGGATGGAC GGGTGAGCAC TGCCAGCTTC GAGACTTCTG CTCAGCCAAC
    CCCTGCGTCA ATGGAGGAGT GTGTCTGGCC ACCTACCCCC AGATCCAGTG CCGCTGCCCA
    CCCGGCTTCG AGGGCCACGC CTGCGAACAC GACGTCAACG AGTGTTACCT GGACCCGGGA
    CCGTGCCCCA AGGGCACCAC CTGCCATAAC ACCCTGGGAT CCTGCCAGTG TCTCTGCCCT
    GCTGGGCGGG AGGGTCCACG CTGTGGACTC CGGCCGGGAC CCTGCACCCC CAGGGGCTGT
    CTCAATGGGG GCACCTGTCA ACTGGTTCCA GGGAGAGACT CCACTTTCCA CCTGTGCCTC
    TGTCCCCCAG GCTTCACAGG CCCAAGCTGT GAGGTGAATC CAGATGACTG TGCTGGGCAC
    CAGTGTCAGA ACGGGGGCAC CTGCCAGGAT GGGCTGGGCA CCTACACCTG CCTCTGCCCA
    GAGGCCTGGA CAGGCTGGGA TTGCTCTGAA GATGTGGACG AATGTGAGGT TCAGGGTCCC
    CCTCGCTGCA GAAATGGGGG TACCTGCCAG AACTCAGCTG GAGACTTCTA CTGTGTGTGT
    GTGAGTGGCT GGGGAGGCGC CGGCTGCGAA GAGAACCTGG ACGACTGTGT GGCCGCCACC
    TGTGCCCCCG GATCCACCTG CATTGACCGC GTGGGCTCCT TCTCCTGCCT CTGCCCGCCT
    GGGCGCACAG GCCTCCTGTG CCACATGGAG GACATGTGTC TGAGTCAGCC ATGCCATGAG
    GAAGCCCAGT GCAGCACCAA CCCCCTGAGT GGCTCCACAC TCTGCGTATG TCAGCCAGGC
    TACACAGGGC CCACCTGCCA CCAGGACCTG GACGAGTGTC AGATGGCTCA GCAAGGCCCC
    AGTCCCTGTG AACACGGCGG CTCCTGCCTC AACACGCCCG GCTCCTTCGA GTGCCTCTGT
    CCGCCTGGCT ACACGGGTTC CCGCTGCGAG GCCGATCACA ACGAGTGCCT GTCGCAGCCC
    TGCCACAGGG GCGGCACCTG CCTGGACCTG CTCGCCACCT TCCAGTGCCT CTGCCCACCA
    GGCTTAGAAG GGCAGCTCTG TGAGGTGGAG ATAGACGAGT GCGCCTCGGC CCCCTGCTTG
    AACCAGGCTG ACTGCCACGA CCTGCTCAAC GGCTTCCAGT GCATCTGCCA GCCAGGTTTC
    ACTGGCCCCC GGTGTGAGGA GGACATCAAC GAATGTCAAA GCTCTCCCTG TGCCAATGGC
    GGGGAGTGCC AGGACCAGCC TGGATCCTTC CATTGCAAGT GTCCCCCAGG CTTTGAAGGC
    CCACGCTGTC AGGAGGAGGT GGACGAGTGT CTGAGTGGCC CATGCCCCAC CGGAGCCAGC
    TGCCTTGACC TCCCAGGAGC TTTCCTTTGC GTCTGCCCCT CTGGCTTCAC AGGCCATCTC
    TGTGAAATTC CCCTGTGTGC CCCCAACCTG TGCCAGCCCA AACAAAAATG TCAGGATCAG
    GAGGACAATG CCCACTGCCT CTGCCCTGAT GGAAACCCAG GCTGTGTCCC CACAGAAGAC
    AACTGCACCT GCCACCATGG GCACTGCCAG AGATCTTCAT GTGTGTGTGA TGGGGGCTGG
    ACAGGACCAG AGTGTGACAC AGATCTGGGG GGCTGTGTCT CCACCCCCTG TGCCCACGGA
    GGGACCTGCC ACCCCCAGCC CTTTGGCTAC AACTGCACCT GCCCCACAGG TTACACAGGG
    CCCACTTGCA GTGAGGAGGT GACAGCCTGT CACTCAGGGC CCTGTCTCAA TGGTGGTTCC
    TGCAGCCCCA GCCCCGGGGG CTACACCTGC ACCTGCCCTC TGAGCCACAC GGGGCTCCGC
    TGCCAGACCA GCATTGACCA TTGTGCCTCT GCACTGTGCC TCAATGGGGG CACCTGTGTG
    AACAAGCCGG GCACCTTCTC CTGCCTCTGC ACCCCAGGCT TCCAGGGCCC ACGCTGCGAG
    GGAAGGACCC GTCCCAGCTG CGCAGACAGC CCCTGTAGGA ACATGGCAAC CTGCCAAGAC
    AGCCCTCAGG GTCCCCGCTG CCTCTGTCCC CCTGGCTACA CAGGAGGCAG CTGTCAGACG
    CTGATGGACT TATGCGCCCA GAAGCCCTGC CCACAAAATT CCCACTGCCT CCAGACTGGG
    CCCTCCTTCC AGTGCCTGTG CCTCCAGGGA TGGACTGGGC CTCTCTGCAA CCTGCCGCTG
    TCCTCCTGCC AGAAGGTTGC TCTGAGCCAA GGCACAGAAG TCTCTTCCCT GTGCCAGAAC
    GGAGGCGTCT GCATTGACAG CGGCCCCTCC CATTTCTGCC ACTGTCCCCC TGGATTCCAA
    GGCAGCATAT GCCAGGACCA GGTGAACCCC TGTGAGTCCA GGCCCTGCCA GCATGGGGCC
    ACCTGCGTGG CCCAACCCAA CGGTTATCTC TGCCAGTGTG CACCCGGCTA CAGTGGACAG
    AACTGCTCAG AGGAACCGGA CGCATGTCAA TCCCAGCCCT GTCACAACCA GGGGACCTGT
    ATCTCCAAAC CCGGAGGCTT CCACTGTGCC TGCCCGCCAG GCTTTGTGGG GCTGCGCTGT
    GAGGGGGACG TGGATGAGTG TCTGGACCGG CCCTGTCACC CCACAGGCAC TGCAGCCTGC
    CACTCTCTGG CCAATGCCTT CTACTGCCAG TGTCTGCCTG GACACACAGG CCAGTGGTGT
    GAAGTGGAGC TAGACCCCTG CCAGAGCCAG CCCTGTGCCC ACAGCGGGTC ATGTGAGGCC
    ACAGCAGGGC CACCCCCAGG ATTCACCTGC CACTGTCCCC AGGGTTTTGA AGGCCCCACC
    TGCAGCCACA GAGCCCCCTC CTGTGGTCTC CATCACTGCC ACCATGGTGG CCTGTGTCTG
    CCCTCCCCCA AGCCTGGACT CCCACCCCGC TGCGCCTGCC TCAATGGTTA TGGGGGTCCG
    GATTGCCTGA CCCCACCAGC TCCTTCAGGC TGTGGCCCTC CTTCTCCATG CCTACACAAT
    GGCAGCTGCT CAGAGACCCC CGGGCTGGGG GGCCCAGGCT TTCGCTGTTC CTGCCCTGCC
    AGCTCTCCAG GGCCCCGGTG TCAGAGGCCC GGAGTCAAGG GATGCGAGGG CAGAAGTGGA
    GATGGGGCCT GTGATGCTGG CTGCAGTGGC CCAGGAGGAA ACTGGGATGG GGGGGACTGC
    TCCCTGGGGG TCCCAGACCC CTGGAAGGGC TGCCCCTCCC ACTCCCGGTG CTGGCTTCTT
    TTCCGGGACG GGCAGTGCCA CCCACAGTGT GACTCTGAAG AGTGTTTGTT TGATGGCTAC
    GACTGTGAGA CTCCTCCAGC CTGCATTCCA GCCTATGACC AGTACTGCCG CGATCACTTC
    CACAATGGAC ACTGCGAGAA GGGCTGCAAC ACGGCAGAAT GTGGCTGGGA CGGGGGAGAC
    TGCAGGCCGG AAGATGGAAA TTCAGAGTGG GGGCCCTCCC TGGCCTTGTT GGTGGTGCTG
    AGCCCCTCAA CCCTGGACCA GCAGCTGCTC GCCCTGGCCC GGGTGCTGTC CCTGACTCTG
    AGGGTAGGGC TCTGGGTGAG GAAGGACAGT GAAGGCAAGG ACATGGTGTA CCCCTATCCT
    GGAGCCCAAG CAGAAGAGGA GCTAGGAGGA ACCCCAGACC CCTCTCAGCA GGAGAGAGCA
    GCCCCCCAAA CAGAGCCTAC AGGCAAGGAG ACAGATTCCC TGAGCACTGG GTTTGTGGTG
    GTGATGGGTG TGGACTTGTC CCACTGTGGC CCCGAGCGCC CTGCATCCCG CTGCCCCTGG
    GACCCTGGGC TCCTACTCCG CTTCCTTGCT GCAATGGCTG CAGTGGGGGC CCTGGAACCC
    CTACTGCCAG GACCCCTGCT GGCTGCCCAC CCTCGTGCAG GCACCAAGCC CTCCGCCAAC
    CAGCTTCCCT GGCCTGTACT GTGCTCCCCA GTGGTCGGGG TCCTTCTCCT AGCCCTCGGG
    GCTCTTCTCG TCCTCCAGCT CATCCGTCGC AAGCGCCGAG AGCATGGGGC CCTCTGGCTG
    CCCCCTGGGT TCACTCGCAG GCCCCGGACT CAGCCAGTTT CCCGCCGACG TCGACCCCCG
    CTGGGCGAGG ACAGCATCGG CCTCAAGGCA CTGAAAGCAG AGGCAGACAT GGATGAGGAT
    GGTGTTGTGA TGTGCTCAGT CCCCAAGGAA GGAGAGGAGG TGGGCCAGGC TGAAGAAATG
    GCCTCACCCC CCAAGTGCCA GCTCTGGTCT CTGAGCAGTG ACTGCCAAGA GCTCCCCCAG
    GCAGCCATGC TGACTCCTCC CCAGGATTCT GAGATGGATG TCCCTGACGT GGACACCCAG
    GGACCTGATG GGGTGACACC CCTGATGTCA GCAGTCTGCT GTGGGGGCGT GGAGTCCAGG
    ACCTACCAGG AGACATGGCT GGGAAGTCCT GAGCCCTGGG AACCTCTGCT GGAGGGAGGG
    GCCTGTCCCC AGGTTCACAC TGTGGGCACT GGGGAGACAC CCCTGCACCT GGCTGCTCGA
    TTCTCCCGGC CAACCGCTGC CCGCCGCCTC CTCGAGGCTG GAGCAAACCC CAACCAGCCA
    GACCGGGCAG GGCGCACCCC GCTTCACACG GCTGTGGCTG CTGATGCTAG GGAGGTTTGC
    CAGCTCTTAC TCCGCAGCAG ACAGACTGCA GTGGATGCAC GGACAGAGGA TGGGACCACA
    GCCCTGATGC TGGCCGCCAG GCTGGCTGTC GAAGACCTGG TTGAAGAACT GATTGCAGCC
    CAAGCAGATG TGGGGGCCAG AGACAAATGG GGAAAAACAG CGCTGCACTG GGCCGCCGCC
    GTGAACAACG CCCGGGCCGC CCGCTCCCTT CTCCAGGCGG GAGCCGATAA AGACGCACAG
    GATGGCAGGG AGCAGACGCC GCTGTTCCTG GCGGCCCGAG AAGGGGCGAT GGAGGTAGCT
    CAGCTGCTGC TGGGGCTGGG AGCCGCCCAA GGACTACGGG ACCAGGCCGG CTTAACACCC
    GGAGACGTCG CCCGTCAGCG CAACCACTGG GATCTGCTGA CGCTGCTGGA AGGGGCGGGG
    CCACCGGAGG CGCGTCACAA ACCCACGCCG GGCCGCGGCG CCGGCACCTT CCAGCGCGCG
    CGGACCGCGT CCGGAAGCGT GCCCCCGCGT GGGGGCGGGG CTGTGCCGCG CTGCCGGACG
    CTGTCAGCCG GAGCCCGTCC GCAAGCCCGG ACTTTGTCCG TAGACTTGGC CGCGCACGGG
    GGCGGGGCCT ACTCGCACTG CCGGAGCCTA TCGAAAGGAA CAGGAGAAGG CCCGCCCCTC
    CGCGGCCGTA GGTTCTCAGC AGGCATGCGC GGGCCTCGGC CCAACCCTGC AATGGTGCGA
    GGAAGACCCG GGGTCTCGGC CGGGAGCGGG GGCGTGGCTG CAACTGTTGA CTGGCCCTGC
    GACTGGGTGC CCCTGGGAGC CTGCGGCCCC GCTTCCAACA CGCCAATCCC GCCTCCTTGC
    CTAACACCGT CCCCTGAGCG GGGATCCCCT CAAGTTGCCT GGGGTCCCCC AGCCCACCAA
    GTAATACCCT TAAATGCAGG AGGCGAGGGT CAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223726.1
    CDS128..6106
    Translation

    Target ORF information:

    RefSeq Version XM_005223669.3
    Organism Bos taurus(cattle)
    Definition Bos taurus notch 4 (NOTCH4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223669.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCAGCCCC CTTCACTGCT GCTGCCACTG CTGCTGCTGC TGTGTCAGCC AGTCGTCAGG 
    ACCAGAGGGC TGCAGTGCGG GAGTTTCCCA GAACCCTGTG CCAACGGAGG CACCTGCCTG
    AGCCTATCCC AGGGGCAAGG GACCTGCCAG TGTGCCCCTG GCTTCCTGGG TGAGACATGC
    CAGTTTCCTG ACCCCTGCCA GGATGCCCAG CCCTGCCAGA ACGGAGGCAG CTGCCACACC
    TTTCTTCCCA CCCTTCCCGG CTCCCCTGGC ACCCCCTCTC CCATGGCCCC CAGCTTCTTC
    TGCACCTGCC CCTCTGGCTT CACTGGCGAT AGGTGCCAGG CCCAGATCAG GGACCCCTGT
    TCCTCCTTCT GTTCCAAAAT GGGTCGCTGC CACCTCCAGG ACTCGGGCCG CCCACGATGC
    TCCTGCATGC CAGGATGGAC GGGTGAGCAC TGCCAGCTTC GAGACTTCTG CTCAGCCAAC
    CCCTGCGTCA ATGGAGGAGT GTGTCTGGCC ACCTACCCCC AGATCCAGTG CCGCTGCCCA
    CCCGGCTTCG AGGGCCACGC CTGCGAACAC GACGTCAACG AGTGTTACCT GGACCCGGGA
    CCGTGCCCCA AGGGCACCAC CTGCCATAAC ACCCTGGGAT CCTGCCAGTG TCTCTGCCCT
    GCTGGGCGGG AGGGTCCACG CTGTGGACTC CGGCCGGGAC CCTGCACCCC CAGGGGCTGT
    CTCAATGGGG GCACCTGTCA ACTGGTTCCA GGGAGAGACT CCACTTTCCA CCTGTGCCTC
    TGTCCCCCAG GCTTCACAGG CCCAAGCTGT GAGGTGAATC CAGATGACTG TGCTGGGCAC
    CAGTGTCAGA ACGGGGGCAC CTGCCAGGAT GGGCTGGGCA CCTACACCTG CCTCTGCCCA
    GAGGCCTGGA CAGGCTGGGA TTGCTCTGAA GATGTGGACG AATGTGAGGT TCAGGGTCCC
    CCTCGCTGCA GAAATGGGGG TACCTGCCAG AACTCAGCTG GAGACTTCTA CTGTGTGTGT
    GTGAGTGGCT GGGGAGGCGC CGGCTGCGAA GAGAACCTGG ACGACTGTGT GGCCGCCACC
    TGTGCCCCCG GATCCACCTG CATTGACCGC GTGGGCTCCT TCTCCTGCCT CTGCCCGCCT
    GGGCGCACAG GCCTCCTGTG CCACATGGAG GACATGTGTC TGAGTCAGCC ATGCCATGAG
    GAAGCCCAGT GCAGCACCAA CCCCCTGAGT GGCTCCACAC TCTGCGTATG TCAGCCAGGC
    TACACAGGGC CCACCTGCCA CCAGGACCTG GACGAGTGTC AGATGGCTCA GCAAGGCCCC
    AGTCCCTGTG AACACGGCGG CTCCTGCCTC AACACGCCCG GCTCCTTCGA GTGCCTCTGT
    CCGCCTGGCT ACACGGGTTC CCGCTGCGAG GCCGATCACA ACGAGTGCCT GTCGCAGCCC
    TGCCACAGGG GCGGCACCTG CCTGGACCTG CTCGCCACCT TCCAGTGCCT CTGCCCACCA
    GGCTTAGAAG GGCAGCTCTG TGAGGTGGAG ATAGACGAGT GCGCCTCGGC CCCCTGCTTG
    AACCAGGCTG ACTGCCACGA CCTGCTCAAC GGCTTCCAGT GCATCTGCCA GCCAGGTTTC
    ACTGGCCCCC GGTGTGAGGA GGACATCAAC GAATGTCAAA GCTCTCCCTG TGCCAATGGC
    GGGGAGTGCC AGGACCAGCC TGGATCCTTC CATTGCAAGT GTCCCCCAGG CTTTGAAGGC
    CCACGCTGTC AGGAGGAGGT GGACGAGTGT CTGAGTGGCC CATGCCCCAC CGGAGCCAGC
    TGCCTTGACC TCCCAGGAGC TTTCCTTTGC GTCTGCCCCT CTGGCTTCAC AGGCCATCTC
    TGTGAAATTC CCCTGTGTGC CCCCAACCTG TGCCAGCCCA AACAAAAATG TCAGGATCAG
    GAGGACAATG CCCACTGCCT CTGCCCTGAT GGAAACCCAG GCTGTGTCCC CACAGAAGAC
    AACTGCACCT GCCACCATGG GCACTGCCAG AGATCTTCAT GTGTGTGTGA TGGGGGCTGG
    ACAGGACCAG AGTGTGACAC AGATCTGGGG GGCTGTGTCT CCACCCCCTG TGCCCACGGA
    GGGACCTGCC ACCCCCAGCC CTTTGGCTAC AACTGCACCT GCCCCACAGG TTACACAGGG
    CCCACTTGCA GTGAGGAGGT GACAGCCTGT CACTCAGGGC CCTGTCTCAA TGGTGGTTCC
    TGCAGCCCCA GCCCCGGGGG CTACACCTGC ACCTGCCCTC TGAGCCACAC GGGGCTCCGC
    TGCCAGACCA GCATTGACCA TTGTGCCTCT GCACTGTGCC TCAATGGGGG CACCTGTGTG
    AACAAGCCGG GCACCTTCTC CTGCCTCTGC ACCCCAGGCT TCCAGGGCCC ACGCTGCGAG
    GGAAGGACCC GTCCCAGCTG CGCAGACAGC CCCTGTAGGA ACATGGCAAC CTGCCAAGAC
    AGCCCTCAGG GTCCCCGCTG CCTCTGTCCC CCTGGCTACA CAGGAGGCAG CTGTCAGACG
    CTGATGGACT TATGCGCCCA GAAGCCCTGC CCACAAAATT CCCACTGCCT CCAGACTGGG
    CCCTCCTTCC AGTGCCTGTG CCTCCAGGGA TGGACTGGGC CTCTCTGCAA CCTGCCGCTG
    TCCTCCTGCC AGAAGGTTGC TCTGAGCCAA GGCACAGAAG TCTCTTCCCT GTGCCAGAAC
    GGAGGCGTCT GCATTGACAG CGGCCCCTCC CATTTCTGCC ACTGTCCCCC TGGATTCCAA
    GGCAGCATAT GCCAGGACCA GGTGAACCCC TGTGAGTCCA GGCCCTGCCA GCATGGGGCC
    ACCTGCGTGG CCCAACCCAA CGGTTATCTC TGCCAGTGTG CACCCGGCTA CAGTGGACAG
    AACTGCTCAG AGGAACCGGA CGCATGTCAA TCCCAGCCCT GTCACAACCA GGGGACCTGT
    ATCTCCAAAC CCGGAGGCTT CCACTGTGCC TGCCCGCCAG GCTTTGTGGG GCTGCGCTGT
    GAGGGGGACG TGGATGAGTG TCTGGACCGG CCCTGTCACC CCACAGGCAC TGCAGCCTGC
    CACTCTCTGG CCAATGCCTT CTACTGCCAG TGTCTGCCTG GACACACAGG CCAGTGGTGT
    GAAGTGGAGC TAGACCCCTG CCAGAGCCAG CCCTGTGCCC ACAGCGGGTC ATGTGAGGCC
    ACAGCAGGGC CACCCCCAGG ATTCACCTGC CACTGTCCCC AGGGTTTTGA AGGCCCCACC
    TGCAGCCACA GAGCCCCCTC CTGTGGTCTC CATCACTGCC ACCATGGTGG CCTGTGTCTG
    CCCTCCCCCA AGCCTGGACT CCCACCCCGC TGCGCCTGCC TCAATGGTTA TGGGGGTCCG
    GATTGCCTGA CCCCACCAGC TCCTTCAGGC TGTGGCCCTC CTTCTCCATG CCTACACAAT
    GGCAGCTGCT CAGAGACCCC CGGGCTGGGG GGCCCAGGCT TTCGCTGTTC CTGCCCTGCC
    AGCTCTCCAG GGCCCCGGTG TCAGAGGCCC GGAGTCAAGG GATGCGAGGG CAGAAGTGGA
    GATGGGGCCT GTGATGCTGG CTGCAGTGGC CCAGGAGGAA ACTGGGATGG GGGGGACTGC
    TCCCTGGGGG TCCCAGACCC CTGGAAGGGC TGCCCCTCCC ACTCCCGGTG CTGGCTTCTT
    TTCCGGGACG GGCAGTGCCA CCCACAGTGT GACTCTGAAG AGTGTTTGTT TGATGGCTAC
    GACTGTGAGA CTCCTCCAGC CTGCATTCCA GCCTATGACC AGTACTGCCG CGATCACTTC
    CACAATGGAC ACTGCGAGAA GGGCTGCAAC ACGGCAGAAT GTGGCTGGGA CGGGGGAGAC
    TGCAGGCCGG AAGATGGAAA TTCAGAGTGG GGGCCCTCCC TGGCCTTGTT GGTGGTGCTG
    AGCCCCTCAA CCCTGGACCA GCAGCTGCTC GCCCTGGCCC GGGTGCTGTC CCTGACTCTG
    AGGGTAGGGC TCTGGGTGAG GAAGGACAGT GAAGGCAAGG ACATGGTGTA CCCCTATCCT
    GGAGCCCAAG CAGAAGAGGA GCTAGGAGGA ACCCCAGACC CCTCTCAGCA GGAGAGAGCA
    GCCCCCCAAA CAGAGCCTAC AGGCAAGGAG ACAGATTCCC TGAGCACTGG GTTTGTGGTG
    GTGATGGGTG TGGACTTGTC CCACTGTGGC CCCGAGCGCC CTGCATCCCG CTGCCCCTGG
    GACCCTGGGC TCCTACTCCG CTTCCTTGCT GCAATGGCTG CAGTGGGGGC CCTGGAACCC
    CTACTGCCAG GACCCCTGCT GGCTGCCCAC CCTCGTGCAG GCACCAAGCC CTCCGCCAAC
    CAGCTTCCCT GGCCTGTACT GTGCTCCCCA GTGGTCGGGG TCCTTCTCCT AGCCCTCGGG
    GCTCTTCTCG TCCTCCAGCT CATCCGTCGC AAGCGCCGAG AGCATGGGGC CCTCTGGCTG
    CCCCCTGGGT TCACTCGCAG GCCCCGGACT CAGCCAGTTT CCCGCCGACG TCGACCCCCG
    CTGGGCGAGG ACAGCATCGG CCTCAAGGCA CTGAAAGCAG AGGCAGACAT GGATGAGGAT
    GGTGTTGTGA TGTGCTCAGT CCCCAAGGAA GGAGAGGAGG TGGGCCAGGC TGAAGAAATG
    GCCTCACCCC CCAAGTGCCA GCTCTGGTCT CTGAGCAGTG ACTGCCAAGA GCTCCCCCAG
    GCAGCCATGC TGACTCCTCC CCAGGATTCT GAGATGGATG TCCCTGACGT GGACACCCAG
    GGACCTGATG GGGTGACACC CCTGATGTCA GCAGTCTGCT GTGGGGGCGT GGAGTCCAGG
    ACCTACCAGG AGACATGGCT GGGAAGTCCT GAGCCCTGGG AACCTCTGCT GGAGGGAGGG
    GCCTGTCCCC AGGTTCACAC TGTGGGCACT GGGGAGACAC CCCTGCACCT GGCTGCTCGA
    TTCTCCCGGC CAACCGCTGC CCGCCGCCTC CTCGAGGCTG GAGCAAACCC CAACCAGCCA
    GACCGGGCAG GGCGCACCCC GCTTCACACG GCTGTGGCTG CTGATGCTAG GGAGGTTTGC
    CAGCTCTTAC TCCGCAGCAG ACAGACTGCA GTGGATGCAC GGACAGAGGA TGGGACCACA
    GCCCTGATGC TGGCCGCCAG GCTGGCTGTC GAAGACCTGG TTGAAGAACT GATTGCAGCC
    CAAGCAGATG TGGGGGCCAG AGACAAATGG GGAAAAACAG CGCTGCACTG GGCCGCCGCC
    GTGAACAACG CCCGGGCCGC CCGCTCCCTT CTCCAGGCGG GAGCCGATAA AGACGCACAG
    GATGGCAGGG AGCAGACGCC GCTGTTCCTG GCGGCCCGAG AAGGGGCGAT GGAGGTAGCT
    CAGCTGCTGC TGGGGCTGGG AGCCGCCCAA GGACTACGGG ACCAGGCCGG CTTAACACCC
    GGAGACGTCG CCCGTCAGCG CAACCACTGG GATCTGCTGA CGCTGCTGGA AGGGGCGGGG
    CCACCGGAGG CGCGTCACAA ACCCACGCCG GGCCGCGGCG CCGGCACCTT CCAGCGCGCG
    CGGACCGCGT CCGGAAGCGT GCCCCCGCGT GGGGGCGGGG CTGTGCCGCG CTGCCGGACG
    CTGTCAGCCG GAGCCCGTCC GCAAGCCCGG ACTTTGTCCG TAGACTTGGC CGCGCACGGG
    GGCGGGGCCT ACTCGCACTG CCGGAGCCTA TCGAAAGGAA CAGGAGAAGG CCCGCCCCTC
    CGCGGCCGTA GGTTCTCAGC AGGCATGCGC GGGCCTCGGC CCAACCCTGC AATGGTGCGA
    GGAAGACCCG GGGTCTCGGC CGGGAGCGGG GGCGTGGCTG CAACTGTTGA CTGGCCCTGC
    GACTGGGTGC CCCTGGGAGC CTGCGGCCCC GCTTCCAACA CGCCAATCCC GCCTCCTTGC
    CTAACACCGT CCCCTGAGCG GGGATCCCCT CAAGTTGCCT GGGGTCCCCC AGCCCACCAA
    GTAATACCCT TAAATGCAGG AGGCGAGGGT CAAAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.