BRAF cDNA ORF clone, Bos taurus(cattle)

The following BRAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa234059 XM_024991186.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa234060 XM_024991187.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa234061 XM_024991188.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa234062 XM_024991189.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa234063 XM_024991190.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa234064 XM_024991191.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa234065 XM_024991192.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa234066 XM_024991193.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa234067 XM_024991194.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa89788 XM_015470801.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa89788 XM_015463148.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa18923 XM_015463145.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa18923 XM_005205911.4
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa18924 XM_002687002.5
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa18924 XM_015463146.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa18929 XM_015463150.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa18929 XM_015463151.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa18929 XM_005205917.4
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa18929 XM_005205916.4
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa18930 XM_015463152.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa18930 XM_005205918.2
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa89787 XM_015470800.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa89787 XM_015463147.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa89789 XM_015463149.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa89789 XM_015470802.1
Latest version!
Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
View Old Accession Versions >>
OBa18929 XM_005205916.3 Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa18923 XM_005205911.3 Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa18930 XM_005205918.1 Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa18929 XM_005205917.3 Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa234059
    Clone ID Related Accession (Same CDS sequence) XM_024991186.1
    Accession Version XM_024991186.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2394bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAGTAGGA AAGACTCTCC TAAGCAAGAG ACAAAATTCA
    GAAGTTATCA GGGAAAAAGA TAAGCAGATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG CTTCCAAACA
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATTCAGGC AGGGGGATAT
    GAAGCAGATT TGGCTCTTAC ATCAAATAAA AATAGAGTAG AAGTTGGGAT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846954.1
    CDS196..2589
    Translation

    Target ORF information:

    RefSeq Version XM_024991186.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991186.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAGTAGGA AAGACTCTCC TAAGCAAGAG ACAAAATTCA
    GAAGTTATCA GGGAAAAAGA TAAGCAGATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG CTTCCAAACA
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATTCAGGC AGGGGGATAT
    GAAGCAGATT TGGCTCTTAC ATCAAATAAA AATAGAGTAG AAGTTGGGAT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234060
    Clone ID Related Accession (Same CDS sequence) XM_024991187.1
    Accession Version XM_024991187.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAGAAAGG TCCAGAAGAA AGTATAGACT CTTTAACAGT
    GGTTACCCCT GGAAGATTCT CGCCTCTATT GAGCTGCTGG CCCGCTCATT GCCAAAAATT
    CACCGCAGTG CATCAGAACC CTCCTTGAAT CGGGCTGGCT TCCAAACAGA GGATTTTAGT
    CTATATGCTT GTGCTTCTCC AAAAACACCC ATTCAGGCAG GGGGATATGA AGCAGATTTG
    GCTCTTACAT CAAATAAAAA TAGAGTAGAA GTTGGGATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846955.1
    CDS196..2577
    Translation

    Target ORF information:

    RefSeq Version XM_024991187.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991187.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAGAAAGG TCCAGAAGAA AGTATAGACT CTTTAACAGT
    GGTTACCCCT GGAAGATTCT CGCCTCTATT GAGCTGCTGG CCCGCTCATT GCCAAAAATT
    CACCGCAGTG CATCAGAACC CTCCTTGAAT CGGGCTGGCT TCCAAACAGA GGATTTTAGT
    CTATATGCTT GTGCTTCTCC AAAAACACCC ATTCAGGCAG GGGGATATGA AGCAGATTTG
    GCTCTTACAT CAAATAAAAA TAGAGTAGAA GTTGGGATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234061
    Clone ID Related Accession (Same CDS sequence) XM_024991188.1
    Accession Version XM_024991188.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAGTAGGA AAGACTCTCC TAAGCAAGAG ACAAAATTCA
    GAAGTTATCA GGGAAAAAGA TAAGCAGATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG CTTCCAAACA
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATTCAGGC AGGGGGATAT
    GGAGAATTTG CAGCCTTCAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846956.1
    CDS196..2559
    Translation

    Target ORF information:

    RefSeq Version XM_024991188.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991188.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAGTAGGA AAGACTCTCC TAAGCAAGAG ACAAAATTCA
    GAAGTTATCA GGGAAAAAGA TAAGCAGATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG CTTCCAAACA
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATTCAGGC AGGGGGATAT
    GGAGAATTTG CAGCCTTCAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234062
    Clone ID Related Accession (Same CDS sequence) XM_024991189.1
    Accession Version XM_024991189.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAGTAGGA AAGACTCTCC TAAGCAAGAG ACAAAATTCA
    GAAGTTATCA GGGAAAAAGA TAAGCAGATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG CTTCCAAACA
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATTCAGGC AGGGGGATAT
    GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846957.1
    CDS196..2541
    Translation

    Target ORF information:

    RefSeq Version XM_024991189.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991189.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAGTAGGA AAGACTCTCC TAAGCAAGAG ACAAAATTCA
    GAAGTTATCA GGGAAAAAGA TAAGCAGATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG CTTCCAAACA
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATTCAGGC AGGGGGATAT
    GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234063
    Clone ID Related Accession (Same CDS sequence) XM_024991190.1
    Accession Version XM_024991190.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGAA
    GCAGATTTGG CTCTTACATC AAATAAAAAT AGAGTAGAAG TTGGGATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846958.1
    CDS196..2526
    Translation

    Target ORF information:

    RefSeq Version XM_024991190.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991190.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGAA
    GCAGATTTGG CTCTTACATC AAATAAAAAT AGAGTAGAAG TTGGGATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234064
    Clone ID Related Accession (Same CDS sequence) XM_024991191.1
    Accession Version XM_024991191.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAAAATTC AGATGTCTTC TGTTAAGGTG TGGAATATCA AACAAATGAT TAAGTTGACA 
    CAGGAGCATA TAGAGGCCCT ATTGGACAAA TTTGGTGGGG AGCATAATCC ACCATCAATA
    TATCTGGAGG CCTATGAAGA ATACACCAGC AAGCTAGATG CCCTCCAACA AAGAGAACAA
    CAGTTATTGG AATCCCTGGG GAATGGAACT GATTTTTCTG TTTCTAGCTC TGCATCAACG
    GACACCGTTA CATCTTCTTC CTCTTCTAGC CTTTCAGTGC TGCCTTCATC TCTTTCAGTT
    TTTCAAAATC CCACAGATGT GTCACGGAGC AACCCCAAGT CACCACAAAA ACCTATCGTT
    AGAGTCTTCC TGCCCAATAA ACAGAGGACA GTGGTACCTG CACGGTGTGG AGTCACAGTC
    CGGGACAGCC TGAAGAAGGC ACTGATGATG AGAGGTCTAA TCCCAGAGTG CTGTGCTGTT
    TACAGAATTC AGGATGGGGA GAAGAAACCA ATTGGCTGGG ACACTGATAT TTCCTGGCTT
    ACTGGAGAGG AGTTGCATGT AGAAGTGTTG GAGAATGTTC CACTTACAAC ACACAACTTT
    GTACGGAAAA CTTTTTTCAC CTTAGCATTT TGTGACTTCT GTAGAAAGCT GCTTTTCCAG
    GGATTCCGCT GTCAAACATG TGGTTATAAA TTTCACCAGC GTTGTAGTAC AGAGGTTCCA
    CTGATGTGTG TTAATTATGA CCAACTAGAT TTGCTGTTTG TCTCCAAGTT CTTTGAACAC
    CACCCAATAC CACAGGAGGA GGCCTCCTTA GCAGAGACTA CCCTTCCATG TGGCTCATCC
    CCTTCTGCAC CCCCCTCCGA TTCTATTGGG CCCCCAATTC TCACCAGTCC ATCTCCTTCA
    AAATCCATTC CAATTCCACA GCCTTTCCGA CCAGCAGATG AAGATCATCG AAATCAGTTT
    GGACAACGAG ACCGGTCCTC ATCAGCTCCA AATGTGCATA TAAACACAAT AGAACCCGTC
    AATATTGATG ACTTGATTAG AGACCAAGGG TTTCGTAGTG ATGGAGGATC AACCACAGGT
    TTATCCGCCA CACCCCCTGC CTCATTACCT GGCTCACTCT CTAATGTGAA AGCATTGCAG
    AAATCTCCAG GACCTCAGCG AGAAAGAAAG TCCTCTTCAT CCTCAGAAGA CAGGAATCGA
    ATGAAAACGC TTGGTAGACG GGATTCAAGT GACGATTGGG AGATTCCTGA TGGACAGATC
    ACAGTGGGAC AAAGAATTGG ATCAGGGTCA TTTGGGACAG TCTACAAGGG AAAGTGGCAT
    GGTGATGTGG CAGTGAAAAT GTTGAATGTG ACAGCACCCA CACCTCAGCA GTTACAGGCC
    TTCAAAAATG AAGTAGGAGT ACTCAGGAAA ACGCGACATG TGAATATCCT CCTCTTCATG
    GGTTATTCAA CAAAGCCACA ACTGGCTATT GTTACCCAGT GGTGTGAGGG CTCCAGTTTA
    TATCATCATC TCCACATCAT TGAGACCAAA TTCGAGATGA TCAAACTTAT AGATATTGCA
    CGGCAGACTG CACAGGGCAT GGATTACTTA CACGCCAAGT CAATCATCCA CAGAGACCTC
    AAGAGTAATA ATATTTTTCT TCATGAAGAC CTCACAGTAA AAATAGGTGA TTTTGGTCTA
    GCCACAGTGA AATCTCGATG GAGTGGGTCC CATCAGTTTG AACAGTTGTC TGGATCCATT
    TTGTGGATGG CACCAGAAGT AATCAGAATG CAAGATAAAA ACCCATATAG CTTTCAGTCA
    GATGTATATG CATTTGGGAT TGTTCTGTAT GAATTGATGA CCGGACAGTT ACCTTATTCA
    AATATCAACA ACAGGGACCA GATAATTTTT ATGGTGGGAC GAGGATATCT GTCTCCAGAT
    CTCAGTAAGG TACGGAGTAA CTGTCCAAAA GCCATGAAGA GATTAATGGC AGAGTGCCTA
    AAAAAGAAAA GAGATGAAAG ACCACTCTTT CCCCAAGTAG GAAAGACTCT CCTAAGCAAG
    AGACAAAATT CAGAAGTTAT CAGGGAAAAA GATAAGCAGA TTCTCGCCTC TATTGAGCTG
    CTGGCCCGCT CATTGCCAAA AATTCACCGC AGTGCATCAG AACCCTCCTT GAATCGGGCT
    GGCTTCCAAA CAGAGGATTT TAGTCTATAT GCTTGTGCTT CTCCAAAAAC ACCCATTCAG
    GCAGGGGGAT ATGAAGCAGA TTTGGCTCTT ACATCAAATA AAAATAGAGT AGAAGTTGGG
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846959.1
    CDS18..2303
    Translation

    Target ORF information:

    RefSeq Version XM_024991191.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991191.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAAAATTC AGATGTCTTC TGTTAAGGTG TGGAATATCA AACAAATGAT TAAGTTGACA 
    CAGGAGCATA TAGAGGCCCT ATTGGACAAA TTTGGTGGGG AGCATAATCC ACCATCAATA
    TATCTGGAGG CCTATGAAGA ATACACCAGC AAGCTAGATG CCCTCCAACA AAGAGAACAA
    CAGTTATTGG AATCCCTGGG GAATGGAACT GATTTTTCTG TTTCTAGCTC TGCATCAACG
    GACACCGTTA CATCTTCTTC CTCTTCTAGC CTTTCAGTGC TGCCTTCATC TCTTTCAGTT
    TTTCAAAATC CCACAGATGT GTCACGGAGC AACCCCAAGT CACCACAAAA ACCTATCGTT
    AGAGTCTTCC TGCCCAATAA ACAGAGGACA GTGGTACCTG CACGGTGTGG AGTCACAGTC
    CGGGACAGCC TGAAGAAGGC ACTGATGATG AGAGGTCTAA TCCCAGAGTG CTGTGCTGTT
    TACAGAATTC AGGATGGGGA GAAGAAACCA ATTGGCTGGG ACACTGATAT TTCCTGGCTT
    ACTGGAGAGG AGTTGCATGT AGAAGTGTTG GAGAATGTTC CACTTACAAC ACACAACTTT
    GTACGGAAAA CTTTTTTCAC CTTAGCATTT TGTGACTTCT GTAGAAAGCT GCTTTTCCAG
    GGATTCCGCT GTCAAACATG TGGTTATAAA TTTCACCAGC GTTGTAGTAC AGAGGTTCCA
    CTGATGTGTG TTAATTATGA CCAACTAGAT TTGCTGTTTG TCTCCAAGTT CTTTGAACAC
    CACCCAATAC CACAGGAGGA GGCCTCCTTA GCAGAGACTA CCCTTCCATG TGGCTCATCC
    CCTTCTGCAC CCCCCTCCGA TTCTATTGGG CCCCCAATTC TCACCAGTCC ATCTCCTTCA
    AAATCCATTC CAATTCCACA GCCTTTCCGA CCAGCAGATG AAGATCATCG AAATCAGTTT
    GGACAACGAG ACCGGTCCTC ATCAGCTCCA AATGTGCATA TAAACACAAT AGAACCCGTC
    AATATTGATG ACTTGATTAG AGACCAAGGG TTTCGTAGTG ATGGAGGATC AACCACAGGT
    TTATCCGCCA CACCCCCTGC CTCATTACCT GGCTCACTCT CTAATGTGAA AGCATTGCAG
    AAATCTCCAG GACCTCAGCG AGAAAGAAAG TCCTCTTCAT CCTCAGAAGA CAGGAATCGA
    ATGAAAACGC TTGGTAGACG GGATTCAAGT GACGATTGGG AGATTCCTGA TGGACAGATC
    ACAGTGGGAC AAAGAATTGG ATCAGGGTCA TTTGGGACAG TCTACAAGGG AAAGTGGCAT
    GGTGATGTGG CAGTGAAAAT GTTGAATGTG ACAGCACCCA CACCTCAGCA GTTACAGGCC
    TTCAAAAATG AAGTAGGAGT ACTCAGGAAA ACGCGACATG TGAATATCCT CCTCTTCATG
    GGTTATTCAA CAAAGCCACA ACTGGCTATT GTTACCCAGT GGTGTGAGGG CTCCAGTTTA
    TATCATCATC TCCACATCAT TGAGACCAAA TTCGAGATGA TCAAACTTAT AGATATTGCA
    CGGCAGACTG CACAGGGCAT GGATTACTTA CACGCCAAGT CAATCATCCA CAGAGACCTC
    AAGAGTAATA ATATTTTTCT TCATGAAGAC CTCACAGTAA AAATAGGTGA TTTTGGTCTA
    GCCACAGTGA AATCTCGATG GAGTGGGTCC CATCAGTTTG AACAGTTGTC TGGATCCATT
    TTGTGGATGG CACCAGAAGT AATCAGAATG CAAGATAAAA ACCCATATAG CTTTCAGTCA
    GATGTATATG CATTTGGGAT TGTTCTGTAT GAATTGATGA CCGGACAGTT ACCTTATTCA
    AATATCAACA ACAGGGACCA GATAATTTTT ATGGTGGGAC GAGGATATCT GTCTCCAGAT
    CTCAGTAAGG TACGGAGTAA CTGTCCAAAA GCCATGAAGA GATTAATGGC AGAGTGCCTA
    AAAAAGAAAA GAGATGAAAG ACCACTCTTT CCCCAAGTAG GAAAGACTCT CCTAAGCAAG
    AGACAAAATT CAGAAGTTAT CAGGGAAAAA GATAAGCAGA TTCTCGCCTC TATTGAGCTG
    CTGGCCCGCT CATTGCCAAA AATTCACCGC AGTGCATCAG AACCCTCCTT GAATCGGGCT
    GGCTTCCAAA CAGAGGATTT TAGTCTATAT GCTTGTGCTT CTCCAAAAAC ACCCATTCAG
    GCAGGGGGAT ATGAAGCAGA TTTGGCTCTT ACATCAAATA AAAATAGAGT AGAAGTTGGG
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234065
    Clone ID Related Accession (Same CDS sequence) XM_024991192.1
    Accession Version XM_024991192.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGAGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAGTAGGA AAGACTCTCC TAAGCAAGAG ACAAAATTCA
    GAAGTTATCA GGGAAAAAGA TAAGCAGATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG CTTCCAAACA
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATTCAGGC AGGGGGATAT
    GAAGCAGATT TGGCTCTTAC ATCAAATAAA AATAGAGTAG AAGTTGGGAT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846960.1
    CDS196..2469
    Translation

    Target ORF information:

    RefSeq Version XM_024991192.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991192.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGAGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAGTAGGA AAGACTCTCC TAAGCAAGAG ACAAAATTCA
    GAAGTTATCA GGGAAAAAGA TAAGCAGATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA
    TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG CTTCCAAACA
    GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATTCAGGC AGGGGGATAT
    GAAGCAGATT TGGCTCTTAC ATCAAATAAA AATAGAGTAG AAGTTGGGAT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234066
    Clone ID Related Accession (Same CDS sequence) XM_024991193.1
    Accession Version XM_024991193.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAGAAAGG TCCAGAAGAA AGTATAGACT CTTTAACAGT
    GGTTACCCCT GGAAGGAAAA GTATGTTTCT TTAGTACATT CCAGGCATTT GGGATTACAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846961.1
    CDS196..2418
    Translation

    Target ORF information:

    RefSeq Version XM_024991193.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991193.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAGAAAGG TCCAGAAGAA AGTATAGACT CTTTAACAGT
    GGTTACCCCT GGAAGGAAAA GTATGTTTCT TTAGTACATT CCAGGCATTT GGGATTACAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234067
    Clone ID Related Accession (Same CDS sequence) XM_024991194.1
    Accession Version XM_024991194.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846962.1
    CDS196..1890
    Translation

    Target ORF information:

    RefSeq Version XM_024991194.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991194.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89788
    Clone ID Related Accession (Same CDS sequence) XM_015470801.1 , XM_015463148.1
    Accession Version XM_015470801.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2193bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAAAATTC AGATGTCTTC TGTTAAGGTG TGGAATATCA AACAAATGAT TAAGTTGACA 
    CAGGAGCATA TAGAGGCCCT ATTGGACAAA TTTGGTGGGG AGCATAATCC ACCATCAATA
    TATCTGGAGG CCTATGAAGA ATACACCAGC AAGCTAGATG CCCTCCAACA AAGAGAACAA
    CAGTTATTGG AATCCCTGGG GAATGGAACT GATTTTTCTG TTTCTAGCTC TGCATCAACG
    GACACCGTTA CATCTTCTTC CTCTTCTAGC CTTTCAGTGC TGCCTTCATC TCTTTCAGTT
    TTTCAAAATC CCACAGATGT GTCACGGAGC AACCCCAAGT CACCACAAAA ACCTATCGTT
    AGAGTCTTCC TGCCCAATAA ACAGAGGACA GTGGTACCTG CACGGTGTGG AGTCACAGTC
    CGGGACAGCC TGAAGAAGGC ACTGATGATG AGAGGTCTAA TCCCAGAGTG CTGTGCTGTT
    TACAGAATTC AGGATGGGGA GAAGAAACCA ATTGGCTGGG ACACTGATAT TTCCTGGCTT
    ACTGGAGAGG AGTTGCATGT AGAAGTGTTG GAGAATGTTC CACTTACAAC ACACAACTTT
    GTACGGAAAA CTTTTTTCAC CTTAGCATTT TGTGACTTCT GTAGAAAGCT GCTTTTCCAG
    GGATTCCGCT GTCAAACATG TGGTTATAAA TTTCACCAGC GTTGTAGTAC AGAGGTTCCA
    CTGATGTGTG TTAATTATGA CCAACTAGAT TTGCTGTTTG TCTCCAAGTT CTTTGAACAC
    CACCCAATAC CACAGGAGGA GGCCTCCTTA GCAGAGACTA CCCTTCCATG TGGCTCATCC
    CCTTCTGCAC CCCCCTCCGA TTCTATTGGG CCCCCAATTC TCACCAGTCC ATCTCCTTCA
    AAATCCATTC CAATTCCACA GCCTTTCCGA CCAGCAGATG AAGATCATCG AAATCAGTTT
    GGACAACGAG ACCGGTCCTC ATCAGCTCCA AATGTGCATA TAAACACAAT AGAACCCGTC
    AATATTGATG ACTTGATTAG AGACCAAGGG TTTCGTAGTG ATGGAGGATC AACCACAGGT
    TTATCCGCCA CACCCCCTGC CTCATTACCT GGCTCACTCT CTAATGTGAA AGCATTGCAG
    AAATCTCCAG GACCTCAGCG AGAAAGAAAG TCCTCTTCAT CCTCAGAAGA CAGGAATCGA
    ATGAAAACGC TTGGTAGACG GGATTCAAGT GACGATTGGG AGATTCCTGA TGGACAGATC
    ACAGTGGGAC AAAGAATTGG ATCAGGGTCA TTTGGGACAG TCTACAAGGG AAAGTGGCAT
    GGTGATGTGG CAGTGAAAAT GTTGAATGTG ACAGCACCCA CACCTCAGCA GTTACAGGCC
    TTCAAAAATG AAGTAGGAGT ACTCAGGAAA ACGCGACATG TGAATATCCT CCTCTTCATG
    GGTTATTCAA CAAAGCCACA ACTGGCTATT GTTACCCAGT GGTGTGAGGG CTCCAGTTTA
    TATCATCATC TCCACATCAT TGAGACCAAA TTCGAGATGA TCAAACTTAT AGATATTGCA
    CGGCAGACTG CACAGGGCAT GGATTACTTA CACGCCAAGT CAATCATCCA CAGAGACCTC
    AAGAGTAATA ATATTTTTCT TCATGAAGAC CTCACAGTAA AAATAGGTGA TTTTGGTCTA
    GCCACAGTGA AATCTCGATG GAGTGGGTCC CATCAGTTTG AACAGTTGTC TGGATCCATT
    TTGTGGATGG CACCAGAAGT AATCAGAATG CAAGATAAAA ACCCATATAG CTTTCAGTCA
    GATGTATATG CATTTGGGAT TGTTCTGTAT GAATTGATGA CCGGACAGTT ACCTTATTCA
    AATATCAACA ACAGGGACCA GATAATTTTT ATGGTGGGAC GAGGATATCT GTCTCCAGAT
    CTCAGTAAGG TACGGAGTAA CTGTCCAAAA GCCATGAAGA GATTAATGGC AGAGTGCCTA
    AAAAAGAAAA GAGATGAAAG ACCACTCTTT CCCCAAATTC TCGCCTCTAT TGAGCTGCTG
    GCCCGCTCAT TGCCAAAAAT TCACCGCAGT GCATCAGAAC CCTCCTTGAA TCGGGCTGGC
    TTCCAAACAG AGGATTTTAG TCTATATGCT TGTGCTTCTC CAAAAACACC CATTCAGGCA
    GGGGGATATG GAGAATTTGC AGCCTTCAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326287.1
    CDS58..2250
    Translation

    Target ORF information:

    RefSeq Version XM_015470801.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470801.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAAAATTC AGATGTCTTC TGTTAAGGTG TGGAATATCA AACAAATGAT TAAGTTGACA 
    CAGGAGCATA TAGAGGCCCT ATTGGACAAA TTTGGTGGGG AGCATAATCC ACCATCAATA
    TATCTGGAGG CCTATGAAGA ATACACCAGC AAGCTAGATG CCCTCCAACA AAGAGAACAA
    CAGTTATTGG AATCCCTGGG GAATGGAACT GATTTTTCTG TTTCTAGCTC TGCATCAACG
    GACACCGTTA CATCTTCTTC CTCTTCTAGC CTTTCAGTGC TGCCTTCATC TCTTTCAGTT
    TTTCAAAATC CCACAGATGT GTCACGGAGC AACCCCAAGT CACCACAAAA ACCTATCGTT
    AGAGTCTTCC TGCCCAATAA ACAGAGGACA GTGGTACCTG CACGGTGTGG AGTCACAGTC
    CGGGACAGCC TGAAGAAGGC ACTGATGATG AGAGGTCTAA TCCCAGAGTG CTGTGCTGTT
    TACAGAATTC AGGATGGGGA GAAGAAACCA ATTGGCTGGG ACACTGATAT TTCCTGGCTT
    ACTGGAGAGG AGTTGCATGT AGAAGTGTTG GAGAATGTTC CACTTACAAC ACACAACTTT
    GTACGGAAAA CTTTTTTCAC CTTAGCATTT TGTGACTTCT GTAGAAAGCT GCTTTTCCAG
    GGATTCCGCT GTCAAACATG TGGTTATAAA TTTCACCAGC GTTGTAGTAC AGAGGTTCCA
    CTGATGTGTG TTAATTATGA CCAACTAGAT TTGCTGTTTG TCTCCAAGTT CTTTGAACAC
    CACCCAATAC CACAGGAGGA GGCCTCCTTA GCAGAGACTA CCCTTCCATG TGGCTCATCC
    CCTTCTGCAC CCCCCTCCGA TTCTATTGGG CCCCCAATTC TCACCAGTCC ATCTCCTTCA
    AAATCCATTC CAATTCCACA GCCTTTCCGA CCAGCAGATG AAGATCATCG AAATCAGTTT
    GGACAACGAG ACCGGTCCTC ATCAGCTCCA AATGTGCATA TAAACACAAT AGAACCCGTC
    AATATTGATG ACTTGATTAG AGACCAAGGG TTTCGTAGTG ATGGAGGATC AACCACAGGT
    TTATCCGCCA CACCCCCTGC CTCATTACCT GGCTCACTCT CTAATGTGAA AGCATTGCAG
    AAATCTCCAG GACCTCAGCG AGAAAGAAAG TCCTCTTCAT CCTCAGAAGA CAGGAATCGA
    ATGAAAACGC TTGGTAGACG GGATTCAAGT GACGATTGGG AGATTCCTGA TGGACAGATC
    ACAGTGGGAC AAAGAATTGG ATCAGGGTCA TTTGGGACAG TCTACAAGGG AAAGTGGCAT
    GGTGATGTGG CAGTGAAAAT GTTGAATGTG ACAGCACCCA CACCTCAGCA GTTACAGGCC
    TTCAAAAATG AAGTAGGAGT ACTCAGGAAA ACGCGACATG TGAATATCCT CCTCTTCATG
    GGTTATTCAA CAAAGCCACA ACTGGCTATT GTTACCCAGT GGTGTGAGGG CTCCAGTTTA
    TATCATCATC TCCACATCAT TGAGACCAAA TTCGAGATGA TCAAACTTAT AGATATTGCA
    CGGCAGACTG CACAGGGCAT GGATTACTTA CACGCCAAGT CAATCATCCA CAGAGACCTC
    AAGAGTAATA ATATTTTTCT TCATGAAGAC CTCACAGTAA AAATAGGTGA TTTTGGTCTA
    GCCACAGTGA AATCTCGATG GAGTGGGTCC CATCAGTTTG AACAGTTGTC TGGATCCATT
    TTGTGGATGG CACCAGAAGT AATCAGAATG CAAGATAAAA ACCCATATAG CTTTCAGTCA
    GATGTATATG CATTTGGGAT TGTTCTGTAT GAATTGATGA CCGGACAGTT ACCTTATTCA
    AATATCAACA ACAGGGACCA GATAATTTTT ATGGTGGGAC GAGGATATCT GTCTCCAGAT
    CTCAGTAAGG TACGGAGTAA CTGTCCAAAA GCCATGAAGA GATTAATGGC AGAGTGCCTA
    AAAAAGAAAA GAGATGAAAG ACCACTCTTT CCCCAAATTC TCGCCTCTAT TGAGCTGCTG
    GCCCGCTCAT TGCCAAAAAT TCACCGCAGT GCATCAGAAC CCTCCTTGAA TCGGGCTGGC
    TTCCAAACAG AGGATTTTAG TCTATATGCT TGTGCTTCTC CAAAAACACC CATTCAGGCA
    GGGGGATATG GAGAATTTGC AGCCTTCAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89788
    Clone ID Related Accession (Same CDS sequence) XM_015470801.1 , XM_015463148.1
    Accession Version XM_015463148.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2193bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAAAATTC AGATGTCTTC TGTTAAGGTG TGGAATATCA AACAAATGAT TAAGTTGACA 
    CAGGAGCATA TAGAGGCCCT ATTGGACAAA TTTGGTGGGG AGCATAATCC ACCATCAATA
    TATCTGGAGG CCTATGAAGA ATACACCAGC AAGCTAGATG CCCTCCAACA AAGAGAACAA
    CAGTTATTGG AATCCCTGGG GAATGGAACT GATTTTTCTG TTTCTAGCTC TGCATCAACG
    GACACCGTTA CATCTTCTTC CTCTTCTAGC CTTTCAGTGC TGCCTTCATC TCTTTCAGTT
    TTTCAAAATC CCACAGATGT GTCACGGAGC AACCCCAAGT CACCACAAAA ACCTATCGTT
    AGAGTCTTCC TGCCCAATAA ACAGAGGACA GTGGTACCTG CACGGTGTGG AGTCACAGTC
    CGGGACAGCC TGAAGAAGGC ACTGATGATG AGAGGTCTAA TCCCAGAGTG CTGTGCTGTT
    TACAGAATTC AGGATGGGGA GAAGAAACCA ATTGGCTGGG ACACTGATAT TTCCTGGCTT
    ACTGGAGAGG AGTTGCATGT AGAAGTGTTG GAGAATGTTC CACTTACAAC ACACAACTTT
    GTACGGAAAA CTTTTTTCAC CTTAGCATTT TGTGACTTCT GTAGAAAGCT GCTTTTCCAG
    GGATTCCGCT GTCAAACATG TGGTTATAAA TTTCACCAGC GTTGTAGTAC AGAGGTTCCA
    CTGATGTGTG TTAATTATGA CCAACTAGAT TTGCTGTTTG TCTCCAAGTT CTTTGAACAC
    CACCCAATAC CACAGGAGGA GGCCTCCTTA GCAGAGACTA CCCTTCCATG TGGCTCATCC
    CCTTCTGCAC CCCCCTCCGA TTCTATTGGG CCCCCAATTC TCACCAGTCC ATCTCCTTCA
    AAATCCATTC CAATTCCACA GCCTTTCCGA CCAGCAGATG AAGATCATCG AAATCAGTTT
    GGACAACGAG ACCGGTCCTC ATCAGCTCCA AATGTGCATA TAAACACAAT AGAACCCGTC
    AATATTGATG ACTTGATTAG AGACCAAGGG TTTCGTAGTG ATGGAGGATC AACCACAGGT
    TTATCCGCCA CACCCCCTGC CTCATTACCT GGCTCACTCT CTAATGTGAA AGCATTGCAG
    AAATCTCCAG GACCTCAGCG AGAAAGAAAG TCCTCTTCAT CCTCAGAAGA CAGGAATCGA
    ATGAAAACGC TTGGTAGACG GGATTCAAGT GACGATTGGG AGATTCCTGA TGGACAGATC
    ACAGTGGGAC AAAGAATTGG ATCAGGGTCA TTTGGGACAG TCTACAAGGG AAAGTGGCAT
    GGTGATGTGG CAGTGAAAAT GTTGAATGTG ACAGCACCCA CACCTCAGCA GTTACAGGCC
    TTCAAAAATG AAGTAGGAGT ACTCAGGAAA ACGCGACATG TGAATATCCT CCTCTTCATG
    GGTTATTCAA CAAAGCCACA ACTGGCTATT GTTACCCAGT GGTGTGAGGG CTCCAGTTTA
    TATCATCATC TCCACATCAT TGAGACCAAA TTCGAGATGA TCAAACTTAT AGATATTGCA
    CGGCAGACTG CACAGGGCAT GGATTACTTA CACGCCAAGT CAATCATCCA CAGAGACCTC
    AAGAGTAATA ATATTTTTCT TCATGAAGAC CTCACAGTAA AAATAGGTGA TTTTGGTCTA
    GCCACAGTGA AATCTCGATG GAGTGGGTCC CATCAGTTTG AACAGTTGTC TGGATCCATT
    TTGTGGATGG CACCAGAAGT AATCAGAATG CAAGATAAAA ACCCATATAG CTTTCAGTCA
    GATGTATATG CATTTGGGAT TGTTCTGTAT GAATTGATGA CCGGACAGTT ACCTTATTCA
    AATATCAACA ACAGGGACCA GATAATTTTT ATGGTGGGAC GAGGATATCT GTCTCCAGAT
    CTCAGTAAGG TACGGAGTAA CTGTCCAAAA GCCATGAAGA GATTAATGGC AGAGTGCCTA
    AAAAAGAAAA GAGATGAAAG ACCACTCTTT CCCCAAATTC TCGCCTCTAT TGAGCTGCTG
    GCCCGCTCAT TGCCAAAAAT TCACCGCAGT GCATCAGAAC CCTCCTTGAA TCGGGCTGGC
    TTCCAAACAG AGGATTTTAG TCTATATGCT TGTGCTTCTC CAAAAACACC CATTCAGGCA
    GGGGGATATG GAGAATTTGC AGCCTTCAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318634.1
    CDS58..2250
    Translation

    Target ORF information:

    RefSeq Version XM_015463148.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463148.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAAAATTC AGATGTCTTC TGTTAAGGTG TGGAATATCA AACAAATGAT TAAGTTGACA 
    CAGGAGCATA TAGAGGCCCT ATTGGACAAA TTTGGTGGGG AGCATAATCC ACCATCAATA
    TATCTGGAGG CCTATGAAGA ATACACCAGC AAGCTAGATG CCCTCCAACA AAGAGAACAA
    CAGTTATTGG AATCCCTGGG GAATGGAACT GATTTTTCTG TTTCTAGCTC TGCATCAACG
    GACACCGTTA CATCTTCTTC CTCTTCTAGC CTTTCAGTGC TGCCTTCATC TCTTTCAGTT
    TTTCAAAATC CCACAGATGT GTCACGGAGC AACCCCAAGT CACCACAAAA ACCTATCGTT
    AGAGTCTTCC TGCCCAATAA ACAGAGGACA GTGGTACCTG CACGGTGTGG AGTCACAGTC
    CGGGACAGCC TGAAGAAGGC ACTGATGATG AGAGGTCTAA TCCCAGAGTG CTGTGCTGTT
    TACAGAATTC AGGATGGGGA GAAGAAACCA ATTGGCTGGG ACACTGATAT TTCCTGGCTT
    ACTGGAGAGG AGTTGCATGT AGAAGTGTTG GAGAATGTTC CACTTACAAC ACACAACTTT
    GTACGGAAAA CTTTTTTCAC CTTAGCATTT TGTGACTTCT GTAGAAAGCT GCTTTTCCAG
    GGATTCCGCT GTCAAACATG TGGTTATAAA TTTCACCAGC GTTGTAGTAC AGAGGTTCCA
    CTGATGTGTG TTAATTATGA CCAACTAGAT TTGCTGTTTG TCTCCAAGTT CTTTGAACAC
    CACCCAATAC CACAGGAGGA GGCCTCCTTA GCAGAGACTA CCCTTCCATG TGGCTCATCC
    CCTTCTGCAC CCCCCTCCGA TTCTATTGGG CCCCCAATTC TCACCAGTCC ATCTCCTTCA
    AAATCCATTC CAATTCCACA GCCTTTCCGA CCAGCAGATG AAGATCATCG AAATCAGTTT
    GGACAACGAG ACCGGTCCTC ATCAGCTCCA AATGTGCATA TAAACACAAT AGAACCCGTC
    AATATTGATG ACTTGATTAG AGACCAAGGG TTTCGTAGTG ATGGAGGATC AACCACAGGT
    TTATCCGCCA CACCCCCTGC CTCATTACCT GGCTCACTCT CTAATGTGAA AGCATTGCAG
    AAATCTCCAG GACCTCAGCG AGAAAGAAAG TCCTCTTCAT CCTCAGAAGA CAGGAATCGA
    ATGAAAACGC TTGGTAGACG GGATTCAAGT GACGATTGGG AGATTCCTGA TGGACAGATC
    ACAGTGGGAC AAAGAATTGG ATCAGGGTCA TTTGGGACAG TCTACAAGGG AAAGTGGCAT
    GGTGATGTGG CAGTGAAAAT GTTGAATGTG ACAGCACCCA CACCTCAGCA GTTACAGGCC
    TTCAAAAATG AAGTAGGAGT ACTCAGGAAA ACGCGACATG TGAATATCCT CCTCTTCATG
    GGTTATTCAA CAAAGCCACA ACTGGCTATT GTTACCCAGT GGTGTGAGGG CTCCAGTTTA
    TATCATCATC TCCACATCAT TGAGACCAAA TTCGAGATGA TCAAACTTAT AGATATTGCA
    CGGCAGACTG CACAGGGCAT GGATTACTTA CACGCCAAGT CAATCATCCA CAGAGACCTC
    AAGAGTAATA ATATTTTTCT TCATGAAGAC CTCACAGTAA AAATAGGTGA TTTTGGTCTA
    GCCACAGTGA AATCTCGATG GAGTGGGTCC CATCAGTTTG AACAGTTGTC TGGATCCATT
    TTGTGGATGG CACCAGAAGT AATCAGAATG CAAGATAAAA ACCCATATAG CTTTCAGTCA
    GATGTATATG CATTTGGGAT TGTTCTGTAT GAATTGATGA CCGGACAGTT ACCTTATTCA
    AATATCAACA ACAGGGACCA GATAATTTTT ATGGTGGGAC GAGGATATCT GTCTCCAGAT
    CTCAGTAAGG TACGGAGTAA CTGTCCAAAA GCCATGAAGA GATTAATGGC AGAGTGCCTA
    AAAAAGAAAA GAGATGAAAG ACCACTCTTT CCCCAAATTC TCGCCTCTAT TGAGCTGCTG
    GCCCGCTCAT TGCCAAAAAT TCACCGCAGT GCATCAGAAC CCTCCTTGAA TCGGGCTGGC
    TTCCAAACAG AGGATTTTAG TCTATATGCT TGTGCTTCTC CAAAAACACC CATTCAGGCA
    GGGGGATATG GAGAATTTGC AGCCTTCAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18923
    Clone ID Related Accession (Same CDS sequence) XM_005205911.3 , XM_005205911.4 , XM_015463145.1
    Accession Version XM_005205911.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205911.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGA
    GAATTTGCAG CCTTCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205968.1
    CDS195..2495
    Translation

    Target ORF information:

    RefSeq Version XM_005205911.3
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205911.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGA
    GAATTTGCAG CCTTCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18923
    Clone ID Related Accession (Same CDS sequence) XM_005205911.3 , XM_005205911.4 , XM_015463145.1
    Accession Version XM_005205911.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205911.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGA
    GAATTTGCAG CCTTCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205968.1
    CDS216..2516
    Translation

    Target ORF information:

    RefSeq Version XM_005205911.4
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205911.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGA
    GAATTTGCAG CCTTCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18923
    Clone ID Related Accession (Same CDS sequence) XM_005205911.3 , XM_005205911.4 , XM_015463145.1
    Accession Version XM_015463145.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGA
    GAATTTGCAG CCTTCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318631.1
    CDS195..2495
    Translation

    Target ORF information:

    RefSeq Version XM_015463145.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463145.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGA
    GAATTTGCAG CCTTCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18924
    Clone ID Related Accession (Same CDS sequence) XM_015463146.1 , XM_002687002.5
    Accession Version XM_015463146.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGT
    ACGTTTCCTG TTCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318632.1
    CDS195..2492
    Translation

    Target ORF information:

    RefSeq Version XM_015463146.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463146.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGT
    ACGTTTCCTG TTCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18924
    Clone ID Related Accession (Same CDS sequence) XM_015463146.1 , XM_002687002.5
    Accession Version XM_002687002.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002687002.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGT
    ACGTTTCCTG TTCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002687048.1
    CDS195..2492
    Translation

    Target ORF information:

    RefSeq Version XM_002687002.5
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002687002.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGT
    ACGTTTCCTG TTCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18929
    Clone ID Related Accession (Same CDS sequence) XM_015463151.1 , XM_005205916.3 , XM_005205916.4 , XM_015463150.1 , XM_005205917.4 , XM_005205917.3
    Accession Version XM_015463151.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGG TGCTTTGTCC TCCATGGGAG TGTAATAAAT GCTGTGCAAG GGCTTACTTC
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318637.1
    CDS195..2240
    Translation

    Target ORF information:

    RefSeq Version XM_015463151.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463151.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGG TGCTTTGTCC TCCATGGGAG TGTAATAAAT GCTGTGCAAG GGCTTACTTC
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18929
    Clone ID Related Accession (Same CDS sequence) XM_015463151.1 , XM_005205916.3 , XM_005205916.4 , XM_015463150.1 , XM_005205917.4 , XM_005205917.3
    Accession Version XM_005205916.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205916.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGG TGCTTTGTCC TCCATGGGAG TGTAATAAAT GCTGTGCAAG GGCTTACTTC
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205973.1
    CDS195..2240
    Translation

    Target ORF information:

    RefSeq Version XM_005205916.3
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205916.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGG TGCTTTGTCC TCCATGGGAG TGTAATAAAT GCTGTGCAAG GGCTTACTTC
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18929
    Clone ID Related Accession (Same CDS sequence) XM_015463151.1 , XM_005205916.3 , XM_005205916.4 , XM_015463150.1 , XM_005205917.4 , XM_005205917.3
    Accession Version XM_005205916.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205916.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGG TGCTTTGTCC TCCATGGGAG TGTAATAAAT GCTGTGCAAG GGCTTACTTC
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205973.1
    CDS196..2241
    Translation

    Target ORF information:

    RefSeq Version XM_005205916.4
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205916.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGG TGCTTTGTCC TCCATGGGAG TGTAATAAAT GCTGTGCAAG GGCTTACTTC
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18929
    Clone ID Related Accession (Same CDS sequence) XM_015463151.1 , XM_005205916.3 , XM_005205916.4 , XM_015463150.1 , XM_005205917.4 , XM_005205917.3
    Accession Version XM_015463150.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGG TGCTTTGTCC TCCATGGGAG TGTAATAAAT GCTGTGCAAG GGCTTACTTC
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318636.1
    CDS195..2240
    Translation

    Target ORF information:

    RefSeq Version XM_015463150.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463150.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGG TGCTTTGTCC TCCATGGGAG TGTAATAAAT GCTGTGCAAG GGCTTACTTC
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18929
    Clone ID Related Accession (Same CDS sequence) XM_015463151.1 , XM_005205916.3 , XM_005205916.4 , XM_015463150.1 , XM_005205917.4 , XM_005205917.3
    Accession Version XM_005205917.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205917.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGG TGCTTTGTCC TCCATGGGAG TGTAATAAAT GCTGTGCAAG GGCTTACTTC
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205974.1
    CDS196..2241
    Translation

    Target ORF information:

    RefSeq Version XM_005205917.4
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205917.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGG TGCTTTGTCC TCCATGGGAG TGTAATAAAT GCTGTGCAAG GGCTTACTTC
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18929
    Clone ID Related Accession (Same CDS sequence) XM_015463151.1 , XM_005205916.3 , XM_005205916.4 , XM_015463150.1 , XM_005205917.4 , XM_005205917.3
    Accession Version XM_005205917.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205917.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGG TGCTTTGTCC TCCATGGGAG TGTAATAAAT GCTGTGCAAG GGCTTACTTC
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205974.1
    CDS195..2240
    Translation

    Target ORF information:

    RefSeq Version XM_005205917.3
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205917.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGG TGCTTTGTCC TCCATGGGAG TGTAATAAAT GCTGTGCAAG GGCTTACTTC
    CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18930
    Clone ID Related Accession (Same CDS sequence) XM_015463152.1 , XM_005205918.1 , XM_005205918.2
    Accession Version XM_015463152.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGT CTGTGCAAGG AAAAGAGACA AAGCCACGGA TCAGAAGCAC ATGGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318638.1
    CDS195..2234
    Translation

    Target ORF information:

    RefSeq Version XM_015463152.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463152.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGT CTGTGCAAGG AAAAGAGACA AAGCCACGGA TCAGAAGCAC ATGGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18930
    Clone ID Related Accession (Same CDS sequence) XM_015463152.1 , XM_005205918.1 , XM_005205918.2
    Accession Version XM_005205918.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGT CTGTGCAAGG AAAAGAGACA AAGCCACGGA TCAGAAGCAC ATGGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205975.1
    CDS195..2234
    Translation

    Target ORF information:

    RefSeq Version XM_005205918.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205918.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGT CTGTGCAAGG AAAAGAGACA AAGCCACGGA TCAGAAGCAC ATGGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18930
    Clone ID Related Accession (Same CDS sequence) XM_015463152.1 , XM_005205918.1 , XM_005205918.2
    Accession Version XM_005205918.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGT CTGTGCAAGG AAAAGAGACA AAGCCACGGA TCAGAAGCAC ATGGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205975.1
    CDS196..2235
    Translation

    Target ORF information:

    RefSeq Version XM_005205918.2
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205918.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGT CTGTGCAAGG AAAAGAGACA AAGCCACGGA TCAGAAGCAC ATGGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89787
    Clone ID Related Accession (Same CDS sequence) XM_015470800.1 , XM_015463147.1
    Accession Version XM_015470800.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326286.1
    CDS195..2477
    Translation

    Target ORF information:

    RefSeq Version XM_015470800.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470800.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89787
    Clone ID Related Accession (Same CDS sequence) XM_015470800.1 , XM_015463147.1
    Accession Version XM_015463147.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318633.1
    CDS195..2477
    Translation

    Target ORF information:

    RefSeq Version XM_015463147.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463147.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGATTT GCTGTTTGTC TCCAAGTTCT TTGAACACCA CCCAATACCA
    CAGGAGGAGG CCTCCTTAGC AGAGACTACC CTTCCATGTG GCTCATCCCC TTCTGCACCC
    CCCTCCGATT CTATTGGGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89789
    Clone ID Related Accession (Same CDS sequence) XM_015463149.1 , XM_015470802.1
    Accession Version XM_015463149.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGAGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGA
    GAATTTGCAG CCTTCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318635.1
    CDS195..2375
    Translation

    Target ORF information:

    RefSeq Version XM_015463149.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463149.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGAGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGA
    GAATTTGCAG CCTTCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89789
    Clone ID Related Accession (Same CDS sequence) XM_015463149.1 , XM_015470802.1
    Accession Version XM_015470802.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase B-raf isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGAGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGA
    GAATTTGCAG CCTTCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326288.1
    CDS195..2375
    Translation

    Target ORF information:

    RefSeq Version XM_015470802.1
    Organism Bos taurus(cattle)
    Definition Bos taurus B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470802.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCGC TGAGTGGCGG CGGCGGCGGC GGCGGCGGTG GCGCGGAGCA GGGCCAGGCT 
    CTGTTCAACG GGGACATGGA GCCCGAGGCC GGCGCCGCGG CCTCTTCGGC TGCGGACCCC
    GCCATTCCCG AGGAGGTGTG GAATATCAAA CAAATGATTA AGTTGACACA GGAGCATATA
    GAGGCCCTAT TGGACAAATT TGGTGGGGAG CATAATCCAC CATCAATATA TCTGGAGGCC
    TATGAAGAAT ACACCAGCAA GCTAGATGCC CTCCAACAAA GAGAACAACA GTTATTGGAA
    TCCCTGGGGA ATGGAACTGA TTTTTCTGTT TCTAGCTCTG CATCAACGGA CACCGTTACA
    TCTTCTTCCT CTTCTAGCCT TTCAGTGCTG CCTTCATCTC TTTCAGTTTT TCAAAATCCC
    ACAGATGTGT CACGGAGCAA CCCCAAGTCA CCACAAAAAC CTATCGTTAG AGTCTTCCTG
    CCCAATAAAC AGAGGACAGT GGTACCTGCA CGGTGTGGAG TCACAGTCCG GGACAGCCTG
    AAGAAGGCAC TGATGATGAG AGGTCTAATC CCAGAGTGCT GTGCTGTTTA CAGAATTCAG
    GATGGGGAGA AGAAACCAAT TGGCTGGGAC ACTGATATTT CCTGGCTTAC TGGAGAGGAG
    TTGCATGTAG AAGTGTTGGA GAATGTTCCA CTTACAACAC ACAACTTTGT ACGGAAAACT
    TTTTTCACCT TAGCATTTTG TGACTTCTGT AGAAAGCTGC TTTTCCAGGG ATTCCGCTGT
    CAAACATGTG GTTATAAATT TCACCAGCGT TGTAGTACAG AGGTTCCACT GATGTGTGTT
    AATTATGACC AACTAGAGCC CCCAATTCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA
    ATTCCACAGC CTTTCCGACC AGCAGATGAA GATCATCGAA ATCAGTTTGG ACAACGAGAC
    CGGTCCTCAT CAGCTCCAAA TGTGCATATA AACACAATAG AACCCGTCAA TATTGATGAC
    TTGATTAGAG ACCAAGGGTT TCGTAGTGAT GGAGGATCAA CCACAGGTTT ATCCGCCACA
    CCCCCTGCCT CATTACCTGG CTCACTCTCT AATGTGAAAG CATTGCAGAA ATCTCCAGGA
    CCTCAGCGAG AAAGAAAGTC CTCTTCATCC TCAGAAGACA GGAATCGAAT GAAAACGCTT
    GGTAGACGGG ATTCAAGTGA CGATTGGGAG ATTCCTGATG GACAGATCAC AGTGGGACAA
    AGAATTGGAT CAGGGTCATT TGGGACAGTC TACAAGGGAA AGTGGCATGG TGATGTGGCA
    GTGAAAATGT TGAATGTGAC AGCACCCACA CCTCAGCAGT TACAGGCCTT CAAAAATGAA
    GTAGGAGTAC TCAGGAAAAC GCGACATGTG AATATCCTCC TCTTCATGGG TTATTCAACA
    AAGCCACAAC TGGCTATTGT TACCCAGTGG TGTGAGGGCT CCAGTTTATA TCATCATCTC
    CACATCATTG AGACCAAATT CGAGATGATC AAACTTATAG ATATTGCACG GCAGACTGCA
    CAGGGCATGG ATTACTTACA CGCCAAGTCA ATCATCCACA GAGACCTCAA GAGTAATAAT
    ATTTTTCTTC ATGAAGACCT CACAGTAAAA ATAGGTGATT TTGGTCTAGC CACAGTGAAA
    TCTCGATGGA GTGGGTCCCA TCAGTTTGAA CAGTTGTCTG GATCCATTTT GTGGATGGCA
    CCAGAAGTAA TCAGAATGCA AGATAAAAAC CCATATAGCT TTCAGTCAGA TGTATATGCA
    TTTGGGATTG TTCTGTATGA ATTGATGACC GGACAGTTAC CTTATTCAAA TATCAACAAC
    AGGGACCAGA TAATTTTTAT GGTGGGACGA GGATATCTGT CTCCAGATCT CAGTAAGGTA
    CGGAGTAACT GTCCAAAAGC CATGAAGAGA TTAATGGCAG AGTGCCTAAA AAAGAAAAGA
    GATGAAAGAC CACTCTTTCC CCAAATTCTC GCCTCTATTG AGCTGCTGGC CCGCTCATTG
    CCAAAAATTC ACCGCAGTGC ATCAGAACCC TCCTTGAATC GGGCTGGCTT CCAAACAGAG
    GATTTTAGTC TATATGCTTG TGCTTCTCCA AAAACACCCA TTCAGGCAGG GGGATATGGA
    GAATTTGCAG CCTTCAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.