RAPGEF3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAPGEF3
Entrez Gene ID	535383
Full Name	Rap guanine nucleotide exchange factor 3
General protein information	Preferred Names Rap guanine nucleotide exchange factor 3 Names rap guanine nucleotide exchange factor 3 RAP guanine-nucleotide-exchange factor 3 Rap guanine nucleotide exchange factor (GEF) 3
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005206392.4	XP_005206449.1	rap guanine nucleotide exchange factor 3 isoform X1
XM_005206393.2	XP_005206450.1	rap guanine nucleotide exchange factor 3 isoform X2
NM_001080302.1	NP_001073771.1	rap guanine nucleotide exchange factor 3
XM_005206392.3	XP_005206449.1	rap guanine nucleotide exchange factor 3 isoform X1
XM_005206393.1	XP_005206450.1	rap guanine nucleotide exchange factor 3 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAPGEF3	XP_001162479.2
Xenopus tropicalis (tropical clawed frog)	rapgef3	XP_002935702.2
Rattus norvegicus (Norway rat)	Rapgef3	NP_067722.1
Danio rerio (zebrafish)	LOC101886094	XP_005162410.1
	RAPGEF3	XP_001098291.2
Gallus gallus (chicken)	RAPGEF3	XP_424494.4
Bos taurus (cattle)	RAPGEF3	NP_001073771.1
Homo sapiens (human)	RAPGEF3	NP_001092001.1
Canis lupus familiaris (dog)	RAPGEF3	NP_001093414.1
Mus musculus (house mouse)	Rapgef3	NP_001171281.1

Gene Ontology
Bibliography

Related articles in PubMed

Prostacyclin regulates bone growth via the Epac/Rap1 pathway.
Hutchison MR, White PC
Endocrinology156(2)499-510(2015 Feb)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

prostacyclin regulates bone growth via the Epac/Rap1 pathway
Title: Prostacyclin regulates bone growth via the Epac/Rap1 pathway.

The following RAPGEF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa39874	NM_001080302.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa19283	XM_005206392.4 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa19284	XM_005206393.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
OBa19283	XM_005206392.3	Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa19284	XM_005206393.1	Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAPGEF3 ( NM_001080302.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001073771.1 Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), mRNA.

Price & Availability↑

CloneID	OBa39874
Clone ID Related Accession (Same CDS sequence)	NM_001080302.1
Accession Version	NM_001080302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT029845.1. On Jan 16, 2007 this sequence version replaced XM_615454.3. ##Evidence-Data-START## Transcript exon combination :: BT029845.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGTGG GCTGGCCAGG TGAGGGCCGC TGGCAGGTGG GCCTGGCTGT AGAGGGCGAC 
TCGATTCTGG GAGCACCGCC GTTGGGAGGC CTCCCGGACG TGGTGCCGGA GGGGACGCTG 
CTCAACATGG TGCTCAGGAG GATGTATCGG TCCCGAAGCT GCTCCTACCA GCTGCTGCTC 
GAACACCAGC GCCCCAGCTG CATCCAGGGG CTGCGCTGGA CGCCACTCAC CAGCAGCGAG 
GAGTCCCTGG ATTTCAGTGT GAGCCTGGAG CAGGCCTCCG CGGAGCGGGT GCTCAGGGCG 
GGAAAGCAGC TGCATCGACA CCTCCTGGCC AACTGCCCCA ACCTCATCCG AGACAGAAAG 
TACCACCTCC GGCTCCACCG GCAGTGCTGC TCCGGCCGGG AGCTGGTAGA TGGGATCCTG 
GCCCTGGGGC TGGGCGTCCA CTCCCGGAGC CAAGCCGTGG GTATCTGCCA GGTGCTCCTG 
GATGAAGGCG CCCTCTGCCA TGTGAAACAC GACTGGGCCT TCCAGGACCG AGACACCCAG 
TTCTACCGTT TCCCGGGGCC GGAGCCAGAG CCTGTGGGTG TCCATGAGCT AGAGGAAGAG 
CTGGTGGAGG CTCTGGCCCT GTTGTCCCAG CGGGGGCCCG ATGCCCTGCT CACGGTGGCA 
CTTCGAAAGC CCCCTGGTCA GCGCACAGAT GAGGAGCTGG ACCTCATCTT TGAGGAGCTG 
CTGCACATCA AGGCCGTGGC CCACCTCTCC AACTCGGTGA AGCGGGAACT GGCTGCAGTC 
CTGCTCTTTG AACCGCACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGC 
ACCTCGTGGT ACATTATCTG GAAGGGATCG GTCAACGTGG TGACCCATGG CAAGGGCCTG 
GTGACCACCC TGCACGAGGG AGATGACTTC GGACAGCTGG CTCTGGTGAA CGATGCACCT 
CGGGCCGCCA CCATCATCCT GGGAGAAGAC AACTGTCATT TTCTTCGTGT GGACAAGCAG 
GACTTCAACC GTATCATCAA GGATGTGGAA GCAAAGACCA TGAGGCTGGA AGAACATGGC 
AAAGTGGTGT TAGTGCTGGA GAGAACCTCT CAGGGCACTG GCCCTTCTCG TCCCCCAACC 
CCAGGCAGGA ATCGGTATAC AGTGATGTCT GGCACCCCAG AGAAGATCCT AGAACTTCTG 
TTGGAGGCCA TGCGGCCTGA CTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTCCTGA CCCACAGTGT CTTCATGCCC ACTGCCCAGC TCTGTGCTGC CCTCCTGCAC 
CACTTCCACG CGGAGCCCGC CGGGGGCAGC GAGCAGGAGC GCAGCTCCTA CATATGCAAC 
AAGAGGCAGC AGATCCTGCG GCTGGTCAGT CAGTGGGTGA CCCTGTACGG CCCTACGCTC 
CGCACCGACC CTGTGGCCAC CAGCTTCCTC AAGAAACTGT CGGACCTGGT GAGCAGGGAC 
ACCCGGCTTA GCAACCTGCT GCGGGAGCAG TGGCCAGAGA GGCGGCGACA CCACAGGTTG 
GAGAACGGCT GTGGGAACGC GTCTCCGCAG ATGAAGGCCC GGAATGTTCC CGTTTGGCTC 
CCCAGCCAGG ACGAGCCTCT CCCTAGCAGC AACTGTGCCA TCAGAGTTGG GGACAAAGTC 
CCCTATGACA TCTGCCGGCC AGACCACTCC ATGCTAACCC TGCAGCTGCC TGTGACGGCC 
TCAGTGAGAG AGGTGATGGC AGCGTTGGCT CAGGAGGACG GCTGGACTAA GGGGCAGGTG 
CTGGTGAAGG TCAACTCGGC AGGTGATGCC ATTGGCCTGC AGCCAGATGC CCGTGGTGTG 
GCCACATCCC TGGGGCTTAA CGAGCGGCTC TTTGTTGTCA ACCCACAGGA AGTGCACGAG 
CTGACCCCAC ACCCCGATCA GCTGGGGCCC ACTGTGGGCT CTGCCGAGGG GCTGGACCTG 
GTGAGCACCA AGGACCTGGC GGGCCAGCTC ACGGACCACG ACTGGAGCCT CTTCAACAGC 
ATCCACCAGG TGGAGCTGAT CCACTATGTG CTGGGCCCCC AGCACCTGCG GGACGTCACC 
ACCGCCAACC TGGAGCGCTT CATGCGCCGC TTCAACCAGC TGCAGTACTG GGTGGCCACG 
GAGCTGTGTC TCTGCTCTGT GCCCGGCCTA CGGGCCCAGC TGCTCAGGAA GTTCATCAAA 
CTGGCAGCCC ACCTCAAGGA GCAAAAGAAT CTTAATTCCT TCTTTGCCAT CATGTTTGGT 
CTCAGCAACT CAGCCATCAG CCGCCTGGCC CATACCTGGG AGCGGCTGCC CCACAAAGTC 
CGGAAGCTCT ACTCGGCCCT AGAGAGGCTG CTGGACCCCT CATGGAACCA CCGAGTGTAC 
CGTCTGGCCC TCACCAAGCT CTCCCCTCCC CTCATCCCCT TCATGCCCCT TCTTCTCAAA 
GACATGACCT TCATCCATGA GGGAAATCAC ACATTGGTGG AGAATCTCAT CAACTTTGAG 
AAGATGAGAA TGATGGCCAG AGCTGCACGG ATGCTACACC ACTGCCGAAG CCACAGCAAT 
GTGCCTCTGT CACCGCTCAG AAGCCGAGTC TCCCACCTGC ACGAGGACAG CCAGGCGGTC 
AGGGTTTCCA CGTGCTCGGA GCAGTCCCTG AGCACCCGGA GTCCCGCCAG CACCTGGGCT 
TACGTCCAGC AGCTGAAGGT CATCGACAAC CAGCGGGAAC TGTCCCGCCT CTCCCGGGAG 
CTGGAGCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073771.1
CDS	269..3040
Translation	MKVGWPGEGRWQVGLAVEGDSILGAPPLGGLPDVVPEGTLLNMVLRRMYRSRSCSYQLLLEHQRPSCIQGLRWTPLTSSEESLDFSVSLEQASAERVLRAGKQLHRHLLANCPNLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWAFQDRDTQFYRFPGPEPEPVGVHELEEELVEALALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILGEDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGTGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPTAQLCAALLHHFHAEPAGGSEQERSSYICNKRQQILRLVSQWVTLYGPTLRTDPVATSFLKKLSDLVSRDTRLSNLLREQWPERRRHHRLENGCGNASPQMKARNVPVWLPSQDEPLPSSNCAIRVGDKVPYDICRPDHSMLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELTPHPDQLGPTVGSAEGLDLVSTKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNQLQYWVATELCLCSVPGLRAQLLRKFIKLAAHLKEQKNLNSFFAIMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPLIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHSNVPLSPLRSRVSHLHEDSQAVRVSTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	NM_001080302.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080302.1

ORF Insert Sequence:

ATGAAGGTGG GCTGGCCAGG TGAGGGCCGC TGGCAGGTGG GCCTGGCTGT AGAGGGCGAC 
TCGATTCTGG GAGCACCGCC GTTGGGAGGC CTCCCGGACG TGGTGCCGGA GGGGACGCTG 
CTCAACATGG TGCTCAGGAG GATGTATCGG TCCCGAAGCT GCTCCTACCA GCTGCTGCTC 
GAACACCAGC GCCCCAGCTG CATCCAGGGG CTGCGCTGGA CGCCACTCAC CAGCAGCGAG 
GAGTCCCTGG ATTTCAGTGT GAGCCTGGAG CAGGCCTCCG CGGAGCGGGT GCTCAGGGCG 
GGAAAGCAGC TGCATCGACA CCTCCTGGCC AACTGCCCCA ACCTCATCCG AGACAGAAAG 
TACCACCTCC GGCTCCACCG GCAGTGCTGC TCCGGCCGGG AGCTGGTAGA TGGGATCCTG 
GCCCTGGGGC TGGGCGTCCA CTCCCGGAGC CAAGCCGTGG GTATCTGCCA GGTGCTCCTG 
GATGAAGGCG CCCTCTGCCA TGTGAAACAC GACTGGGCCT TCCAGGACCG AGACACCCAG 
TTCTACCGTT TCCCGGGGCC GGAGCCAGAG CCTGTGGGTG TCCATGAGCT AGAGGAAGAG 
CTGGTGGAGG CTCTGGCCCT GTTGTCCCAG CGGGGGCCCG ATGCCCTGCT CACGGTGGCA 
CTTCGAAAGC CCCCTGGTCA GCGCACAGAT GAGGAGCTGG ACCTCATCTT TGAGGAGCTG 
CTGCACATCA AGGCCGTGGC CCACCTCTCC AACTCGGTGA AGCGGGAACT GGCTGCAGTC 
CTGCTCTTTG AACCGCACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGC 
ACCTCGTGGT ACATTATCTG GAAGGGATCG GTCAACGTGG TGACCCATGG CAAGGGCCTG 
GTGACCACCC TGCACGAGGG AGATGACTTC GGACAGCTGG CTCTGGTGAA CGATGCACCT 
CGGGCCGCCA CCATCATCCT GGGAGAAGAC AACTGTCATT TTCTTCGTGT GGACAAGCAG 
GACTTCAACC GTATCATCAA GGATGTGGAA GCAAAGACCA TGAGGCTGGA AGAACATGGC 
AAAGTGGTGT TAGTGCTGGA GAGAACCTCT CAGGGCACTG GCCCTTCTCG TCCCCCAACC 
CCAGGCAGGA ATCGGTATAC AGTGATGTCT GGCACCCCAG AGAAGATCCT AGAACTTCTG 
TTGGAGGCCA TGCGGCCTGA CTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTCCTGA CCCACAGTGT CTTCATGCCC ACTGCCCAGC TCTGTGCTGC CCTCCTGCAC 
CACTTCCACG CGGAGCCCGC CGGGGGCAGC GAGCAGGAGC GCAGCTCCTA CATATGCAAC 
AAGAGGCAGC AGATCCTGCG GCTGGTCAGT CAGTGGGTGA CCCTGTACGG CCCTACGCTC 
CGCACCGACC CTGTGGCCAC CAGCTTCCTC AAGAAACTGT CGGACCTGGT GAGCAGGGAC 
ACCCGGCTTA GCAACCTGCT GCGGGAGCAG TGGCCAGAGA GGCGGCGACA CCACAGGTTG 
GAGAACGGCT GTGGGAACGC GTCTCCGCAG ATGAAGGCCC GGAATGTTCC CGTTTGGCTC 
CCCAGCCAGG ACGAGCCTCT CCCTAGCAGC AACTGTGCCA TCAGAGTTGG GGACAAAGTC 
CCCTATGACA TCTGCCGGCC AGACCACTCC ATGCTAACCC TGCAGCTGCC TGTGACGGCC 
TCAGTGAGAG AGGTGATGGC AGCGTTGGCT CAGGAGGACG GCTGGACTAA GGGGCAGGTG 
CTGGTGAAGG TCAACTCGGC AGGTGATGCC ATTGGCCTGC AGCCAGATGC CCGTGGTGTG 
GCCACATCCC TGGGGCTTAA CGAGCGGCTC TTTGTTGTCA ACCCACAGGA AGTGCACGAG 
CTGACCCCAC ACCCCGATCA GCTGGGGCCC ACTGTGGGCT CTGCCGAGGG GCTGGACCTG 
GTGAGCACCA AGGACCTGGC GGGCCAGCTC ACGGACCACG ACTGGAGCCT CTTCAACAGC 
ATCCACCAGG TGGAGCTGAT CCACTATGTG CTGGGCCCCC AGCACCTGCG GGACGTCACC 
ACCGCCAACC TGGAGCGCTT CATGCGCCGC TTCAACCAGC TGCAGTACTG GGTGGCCACG 
GAGCTGTGTC TCTGCTCTGT GCCCGGCCTA CGGGCCCAGC TGCTCAGGAA GTTCATCAAA 
CTGGCAGCCC ACCTCAAGGA GCAAAAGAAT CTTAATTCCT TCTTTGCCAT CATGTTTGGT 
CTCAGCAACT CAGCCATCAG CCGCCTGGCC CATACCTGGG AGCGGCTGCC CCACAAAGTC 
CGGAAGCTCT ACTCGGCCCT AGAGAGGCTG CTGGACCCCT CATGGAACCA CCGAGTGTAC 
CGTCTGGCCC TCACCAAGCT CTCCCCTCCC CTCATCCCCT TCATGCCCCT TCTTCTCAAA 
GACATGACCT TCATCCATGA GGGAAATCAC ACATTGGTGG AGAATCTCAT CAACTTTGAG 
AAGATGAGAA TGATGGCCAG AGCTGCACGG ATGCTACACC ACTGCCGAAG CCACAGCAAT 
GTGCCTCTGT CACCGCTCAG AAGCCGAGTC TCCCACCTGC ACGAGGACAG CCAGGCGGTC 
AGGGTTTCCA CGTGCTCGGA GCAGTCCCTG AGCACCCGGA GTCCCGCCAG CACCTGGGCT 
TACGTCCAGC AGCTGAAGGT CATCGACAAC CAGCGGGAAC TGTCCCGCCT CTCCCGGGAG 
CTGGAGCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF3 ( XM_005206392.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206449.1 Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa19283
Clone ID Related Accession (Same CDS sequence)	XM_005206392.4 , XM_005206392.3
Accession Version	XM_005206392.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206392.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCGGTG GGGTGGGGCA GAATTCCAAA GAAGGGCCTG ATCTCTGGTA CGCCGCCGCC 
GCCGCCGGCT GGAGACCCCT AAGTGCGCCA GACCGGCAGG CCCCAGTGAA GGTCTTCCTG 
TCCCTCCCCC GGCTCCTGGC AGGGGCGCAG CACCTCCGCC TGACCAGTAC AGAGGATTCG 
AAGGAGGTGG GCTGGCCAGG TGAGGGCCGC TGGCAGGTGG GCCTGGCTGT AGAGGGCGAC 
TCGATTCTGG GAGCACCGCC GTTGGGAGGC CTCCCGGACG TGGTGCCGGA GGGGACGCTG 
CTCAACATGG TGCTCAGGAG GATGTATCGG TCCCGAAGCT GCTCCTACCA GCTGCTGCTC 
GAACACCAGC GCCCCAGCTG CATCCAGGGG CTGCGCTGGA CGCCACTCAC CAGCAGCGAG 
GAGTCCCTGG ATTTCAGTGT GAGCCTGGAG CAGGCCTCCG CGGAGCGGGT GCTCAGGGCG 
GGAAAGCAGC TGCATCGACA CCTCCTGGCC AACTGCCCCA ACCTCATCCG AGACAGAAAG 
TACCACCTCC GGCTCCACCG GCAGTGCTGC TCCGGCCGGG AGCTGGTAGA TGGGATCCTG 
GCCCTGGGGC TGGGCGTCCA CTCCCGGAGC CAAGCCGTGG GTATCTGCCA GGTGCTCCTG 
GATGAAGGCG CCCTCTGCCA TGTGAAACAC GACTGGGCCT TCCAGGACCG AGACACCCAG 
TTCTACCGTT TCCCGGGGCC GGAGCCAGAG CCTGTGGGTG TCCATGAGCT AGAGGAAGAG 
CTGGTGGAGG CTCTGGCCCT GTTGTCCCAG CGGGGGCCCG ATGCCCTGCT CACGGTGGCA 
CTTCGAAAGC CCCCTGGTCA GCGCACAGAT GAGGAGCTGG ACCTCATCTT TGAGGAGCTG 
CTGCACATCA AGGCCGTGGC CCACCTCTCC AACTCGGTGA AGCGGGAACT GGCTGCAGTC 
CTGCTCTTTG AACCGCACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGC 
ACCTCGTGGT ACATTATCTG GAAGGGATCG GTCAACGTGG TGACCCATGG CAAGGGCCTG 
GTGACCACCC TGCACGAGGG AGATGACTTC GGACAGCTGG CTCTGGTGAA CGATGCACCT 
CGGGCCGCCA CCATCATCCT GGGAGAAGAC AACTGTCATT TTCTTCGTGT GGACAAGCAG 
GACTTCAACC GTATCATCAA GGATGTGGAA GCAAAGACCA TGAGGCTGGA AGAACATGGC 
AAAGTGGTGT TAGTGCTGGA GAGAACCTCT CAGGGCACTG GCCCTTCTCG TCCCCCAACC 
CCAGGCAGGA ATCGGTATAC AGTGATGTCT GGCACCCCAG AGAAGATCCT AGAACTTCTG 
TTGGAGGCCA TGCGGCCTGA CTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTCCTGA CCCACAGTGT CTTCATGCCC ACTGCCCAGC TCTGTGCTGC CCTCCTGCAC 
CACTTCCACG CGGAGCCCGC CGGGGGCAGC GAGCAGGAGC GCAGCTCCTA CATATGCAAC 
AAGAGGCAGC AGATCCTGCG GCTGGTCAGT CAGTGGGTGA CCCTGTACGG CCCTACGCTC 
CGCACCGACC CTGTGGCCAC CAGCTTCCTC AAGAAACTGT CGGACCTGGT GAGCAGGGAC 
ACCCGGCTTA GCAACCTGCT GCGGGAGCAG TGGCCAGAGA GGCGGCGACA CCACAGGTTG 
GAGAACGGCT GTGGGAACGT GTCTCCGCAG ATGAAGGCCC GGAATGTTCC CGTTTGGCTC 
CCCAGCCAGG ACGAGCCTCT CCCTAGCAGC AACTGTGCCA TCAGAGTTGG GGACAAAGTC 
CCCTATGACA TCTGCCGGCC AGACCACTCC ATGCTAACCC TGCAGCTGCC TGTGACGGCC 
TCAGTGAGAG AGGTGATGGC AGCGTTGGCT CAGGAGGACG GCTGGACTAA GGGGCAGGTG 
CTGGTGAAGG TCAACTCGGC AGGTGATGCC ATTGGCCTGC AGCCAGATGC CCGTGGTGTG 
GCCACATCCC TGGGGCTTAA CGAGCGGCTC TTTGTTGTCA ACCCACAGGA AGTGCACGAG 
CTGACCCCAC ACCCCGATCA GCTGGGGCCC ACTGTGGGCT CTGCCGAGGG GCTGGACCTG 
GTGAGCACCA AGGACCTGGC GGGCCAGCTC ACGGACCACG ACTGGAGCCT CTTCAACAGC 
ATCCACCAGG TGGAGCTGAT CCACTATGTG CTGGGCCCCC AGCACCTGCG GGACGTCACC 
ACCGCCAACC TGGAGCGCTT CATGCGCCGC TTCAACGAGC TGCAGTACTG GGTGGCCACG 
GAGCTGTGTC TCTGCTCTGT GCCCGGCCTA CGGGCCCAGC TGCTCAGGAA GTTCATCAAA 
CTGGCAGCCC ACCTCAAGGA GCAAAAGAAT CTTAATTCCT TCTTTGCCAT CATGTTTGGT 
CTCAGCAACT CAGCCATCAG CCGCCTGGCC CATACCTGGG AGCGGCTGCC CCACAAAGTC 
CGGAAGCTCT ACTCGGCCCT CGAGAGGCTG CTGGACCCCT CATGGAACCA CCGAGTGTAC 
CGTCTGGCCC TCACCAAGCT CTCCCCTCCC CTCATCCCCT TCATGCCCCT TCTTCTCAAA 
GACATGACCT TCATCCATGA GGGAAATCAC ACATTGGTGG AGAATCTCAT CAACTTTGAG 
AAGATGAGAA TGATGGCCAG AGCTGCACGG ATGCTACACC ACTGCCGAAG CCACAGCAAT 
GTGCCTCTGT CACCGCTCAG AAGCCGAGTC TCCCACCTGC ACGAGGACAG CCAGGCGGTC 
AGGGTTTCCA CGTGCTCGGA GCAGTCCCTG AGCACCCGGA GTCCCGCCAG CACCTGGGCT 
TACGTCCAGC AGCTGAAGGT CATCGACAAC CAGCGGGAAC TGTCCCGCCT CTCCCGGGAG 
CTGGAGCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206449.1
CDS	93..3044
Translation	MVGGVGQNSKEGPDLWYAAAAAGWRPLSAPDRQAPVKVFLSLPRLLAGAQHLRLTSTEDSKEVGWPGEGRWQVGLAVEGDSILGAPPLGGLPDVVPEGTLLNMVLRRMYRSRSCSYQLLLEHQRPSCIQGLRWTPLTSSEESLDFSVSLEQASAERVLRAGKQLHRHLLANCPNLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWAFQDRDTQFYRFPGPEPEPVGVHELEEELVEALALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILGEDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGTGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPTAQLCAALLHHFHAEPAGGSEQERSSYICNKRQQILRLVSQWVTLYGPTLRTDPVATSFLKKLSDLVSRDTRLSNLLREQWPERRRHHRLENGCGNVSPQMKARNVPVWLPSQDEPLPSSNCAIRVGDKVPYDICRPDHSMLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELTPHPDQLGPTVGSAEGLDLVSTKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCSVPGLRAQLLRKFIKLAAHLKEQKNLNSFFAIMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPLIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHSNVPLSPLRSRVSHLHEDSQAVRVSTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	XM_005206392.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206392.4

ORF Insert Sequence:

ATGGTCGGTG GGGTGGGGCA GAATTCCAAA GAAGGGCCTG ATCTCTGGTA CGCCGCCGCC 
GCCGCCGGCT GGAGACCCCT AAGTGCGCCA GACCGGCAGG CCCCAGTGAA GGTCTTCCTG 
TCCCTCCCCC GGCTCCTGGC AGGGGCGCAG CACCTCCGCC TGACCAGTAC AGAGGATTCG 
AAGGAGGTGG GCTGGCCAGG TGAGGGCCGC TGGCAGGTGG GCCTGGCTGT AGAGGGCGAC 
TCGATTCTGG GAGCACCGCC GTTGGGAGGC CTCCCGGACG TGGTGCCGGA GGGGACGCTG 
CTCAACATGG TGCTCAGGAG GATGTATCGG TCCCGAAGCT GCTCCTACCA GCTGCTGCTC 
GAACACCAGC GCCCCAGCTG CATCCAGGGG CTGCGCTGGA CGCCACTCAC CAGCAGCGAG 
GAGTCCCTGG ATTTCAGTGT GAGCCTGGAG CAGGCCTCCG CGGAGCGGGT GCTCAGGGCG 
GGAAAGCAGC TGCATCGACA CCTCCTGGCC AACTGCCCCA ACCTCATCCG AGACAGAAAG 
TACCACCTCC GGCTCCACCG GCAGTGCTGC TCCGGCCGGG AGCTGGTAGA TGGGATCCTG 
GCCCTGGGGC TGGGCGTCCA CTCCCGGAGC CAAGCCGTGG GTATCTGCCA GGTGCTCCTG 
GATGAAGGCG CCCTCTGCCA TGTGAAACAC GACTGGGCCT TCCAGGACCG AGACACCCAG 
TTCTACCGTT TCCCGGGGCC GGAGCCAGAG CCTGTGGGTG TCCATGAGCT AGAGGAAGAG 
CTGGTGGAGG CTCTGGCCCT GTTGTCCCAG CGGGGGCCCG ATGCCCTGCT CACGGTGGCA 
CTTCGAAAGC CCCCTGGTCA GCGCACAGAT GAGGAGCTGG ACCTCATCTT TGAGGAGCTG 
CTGCACATCA AGGCCGTGGC CCACCTCTCC AACTCGGTGA AGCGGGAACT GGCTGCAGTC 
CTGCTCTTTG AACCGCACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGC 
ACCTCGTGGT ACATTATCTG GAAGGGATCG GTCAACGTGG TGACCCATGG CAAGGGCCTG 
GTGACCACCC TGCACGAGGG AGATGACTTC GGACAGCTGG CTCTGGTGAA CGATGCACCT 
CGGGCCGCCA CCATCATCCT GGGAGAAGAC AACTGTCATT TTCTTCGTGT GGACAAGCAG 
GACTTCAACC GTATCATCAA GGATGTGGAA GCAAAGACCA TGAGGCTGGA AGAACATGGC 
AAAGTGGTGT TAGTGCTGGA GAGAACCTCT CAGGGCACTG GCCCTTCTCG TCCCCCAACC 
CCAGGCAGGA ATCGGTATAC AGTGATGTCT GGCACCCCAG AGAAGATCCT AGAACTTCTG 
TTGGAGGCCA TGCGGCCTGA CTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTCCTGA CCCACAGTGT CTTCATGCCC ACTGCCCAGC TCTGTGCTGC CCTCCTGCAC 
CACTTCCACG CGGAGCCCGC CGGGGGCAGC GAGCAGGAGC GCAGCTCCTA CATATGCAAC 
AAGAGGCAGC AGATCCTGCG GCTGGTCAGT CAGTGGGTGA CCCTGTACGG CCCTACGCTC 
CGCACCGACC CTGTGGCCAC CAGCTTCCTC AAGAAACTGT CGGACCTGGT GAGCAGGGAC 
ACCCGGCTTA GCAACCTGCT GCGGGAGCAG TGGCCAGAGA GGCGGCGACA CCACAGGTTG 
GAGAACGGCT GTGGGAACGT GTCTCCGCAG ATGAAGGCCC GGAATGTTCC CGTTTGGCTC 
CCCAGCCAGG ACGAGCCTCT CCCTAGCAGC AACTGTGCCA TCAGAGTTGG GGACAAAGTC 
CCCTATGACA TCTGCCGGCC AGACCACTCC ATGCTAACCC TGCAGCTGCC TGTGACGGCC 
TCAGTGAGAG AGGTGATGGC AGCGTTGGCT CAGGAGGACG GCTGGACTAA GGGGCAGGTG 
CTGGTGAAGG TCAACTCGGC AGGTGATGCC ATTGGCCTGC AGCCAGATGC CCGTGGTGTG 
GCCACATCCC TGGGGCTTAA CGAGCGGCTC TTTGTTGTCA ACCCACAGGA AGTGCACGAG 
CTGACCCCAC ACCCCGATCA GCTGGGGCCC ACTGTGGGCT CTGCCGAGGG GCTGGACCTG 
GTGAGCACCA AGGACCTGGC GGGCCAGCTC ACGGACCACG ACTGGAGCCT CTTCAACAGC 
ATCCACCAGG TGGAGCTGAT CCACTATGTG CTGGGCCCCC AGCACCTGCG GGACGTCACC 
ACCGCCAACC TGGAGCGCTT CATGCGCCGC TTCAACGAGC TGCAGTACTG GGTGGCCACG 
GAGCTGTGTC TCTGCTCTGT GCCCGGCCTA CGGGCCCAGC TGCTCAGGAA GTTCATCAAA 
CTGGCAGCCC ACCTCAAGGA GCAAAAGAAT CTTAATTCCT TCTTTGCCAT CATGTTTGGT 
CTCAGCAACT CAGCCATCAG CCGCCTGGCC CATACCTGGG AGCGGCTGCC CCACAAAGTC 
CGGAAGCTCT ACTCGGCCCT CGAGAGGCTG CTGGACCCCT CATGGAACCA CCGAGTGTAC 
CGTCTGGCCC TCACCAAGCT CTCCCCTCCC CTCATCCCCT TCATGCCCCT TCTTCTCAAA 
GACATGACCT TCATCCATGA GGGAAATCAC ACATTGGTGG AGAATCTCAT CAACTTTGAG 
AAGATGAGAA TGATGGCCAG AGCTGCACGG ATGCTACACC ACTGCCGAAG CCACAGCAAT 
GTGCCTCTGT CACCGCTCAG AAGCCGAGTC TCCCACCTGC ACGAGGACAG CCAGGCGGTC 
AGGGTTTCCA CGTGCTCGGA GCAGTCCCTG AGCACCCGGA GTCCCGCCAG CACCTGGGCT 
TACGTCCAGC AGCTGAAGGT CATCGACAAC CAGCGGGAAC TGTCCCGCCT CTCCCGGGAG 
CTGGAGCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF3 ( XM_005206392.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206449.1 Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa19283
Clone ID Related Accession (Same CDS sequence)	XM_005206392.4 , XM_005206392.3
Accession Version	XM_005206392.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103921.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206392.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCGGTG GGGTGGGGCA GAATTCCAAA GAAGGGCCTG ATCTCTGGTA CGCCGCCGCC 
GCCGCCGGCT GGAGACCCCT AAGTGCGCCA GACCGGCAGG CCCCAGTGAA GGTCTTCCTG 
TCCCTCCCCC GGCTCCTGGC AGGGGCGCAG CACCTCCGCC TGACCAGTAC AGAGGATTCG 
AAGGAGGTGG GCTGGCCAGG TGAGGGCCGC TGGCAGGTGG GCCTGGCTGT AGAGGGCGAC 
TCGATTCTGG GAGCACCGCC GTTGGGAGGC CTCCCGGACG TGGTGCCGGA GGGGACGCTG 
CTCAACATGG TGCTCAGGAG GATGTATCGG TCCCGAAGCT GCTCCTACCA GCTGCTGCTC 
GAACACCAGC GCCCCAGCTG CATCCAGGGG CTGCGCTGGA CGCCACTCAC CAGCAGCGAG 
GAGTCCCTGG ATTTCAGTGT GAGCCTGGAG CAGGCCTCCG CGGAGCGGGT GCTCAGGGCG 
GGAAAGCAGC TGCATCGACA CCTCCTGGCC AACTGCCCCA ACCTCATCCG AGACAGAAAG 
TACCACCTCC GGCTCCACCG GCAGTGCTGC TCCGGCCGGG AGCTGGTAGA TGGGATCCTG 
GCCCTGGGGC TGGGCGTCCA CTCCCGGAGC CAAGCCGTGG GTATCTGCCA GGTGCTCCTG 
GATGAAGGCG CCCTCTGCCA TGTGAAACAC GACTGGGCCT TCCAGGACCG AGACACCCAG 
TTCTACCGTT TCCCGGGGCC GGAGCCAGAG CCTGTGGGTG TCCATGAGCT AGAGGAAGAG 
CTGGTGGAGG CTCTGGCCCT GTTGTCCCAG CGGGGGCCCG ATGCCCTGCT CACGGTGGCA 
CTTCGAAAGC CCCCTGGTCA GCGCACAGAT GAGGAGCTGG ACCTCATCTT TGAGGAGCTG 
CTGCACATCA AGGCCGTGGC CCACCTCTCC AACTCGGTGA AGCGGGAACT GGCTGCAGTC 
CTGCTCTTTG AACCGCACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGC 
ACCTCGTGGT ACATTATCTG GAAGGGATCG GTCAACGTGG TGACCCATGG CAAGGGCCTG 
GTGACCACCC TGCACGAGGG AGATGACTTC GGACAGCTGG CTCTGGTGAA CGATGCACCT 
CGGGCCGCCA CCATCATCCT GGGAGAAGAC AACTGTCATT TTCTTCGTGT GGACAAGCAG 
GACTTCAACC GTATCATCAA GGATGTGGAA GCAAAGACCA TGAGGCTGGA AGAACATGGC 
AAAGTGGTGT TAGTGCTGGA GAGAACCTCT CAGGGCACTG GCCCTTCTCG TCCCCCAACC 
CCAGGCAGGA ATCGGTATAC AGTGATGTCT GGCACCCCAG AGAAGATCCT AGAACTTCTG 
TTGGAGGCCA TGCGGCCTGA CTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTCCTGA CCCACAGTGT CTTCATGCCC ACTGCCCAGC TCTGTGCTGC CCTCCTGCAC 
CACTTCCACG CGGAGCCCGC CGGGGGCAGC GAGCAGGAGC GCAGCTCCTA CATATGCAAC 
AAGAGGCAGC AGATCCTGCG GCTGGTCAGT CAGTGGGTGA CCCTGTACGG CCCTACGCTC 
CGCACCGACC CTGTGGCCAC CAGCTTCCTC AAGAAACTGT CGGACCTGGT GAGCAGGGAC 
ACCCGGCTTA GCAACCTGCT GCGGGAGCAG TGGCCAGAGA GGCGGCGACA CCACAGGTTG 
GAGAACGGCT GTGGGAACGT GTCTCCGCAG ATGAAGGCCC GGAATGTTCC CGTTTGGCTC 
CCCAGCCAGG ACGAGCCTCT CCCTAGCAGC AACTGTGCCA TCAGAGTTGG GGACAAAGTC 
CCCTATGACA TCTGCCGGCC AGACCACTCC ATGCTAACCC TGCAGCTGCC TGTGACGGCC 
TCAGTGAGAG AGGTGATGGC AGCGTTGGCT CAGGAGGACG GCTGGACTAA GGGGCAGGTG 
CTGGTGAAGG TCAACTCGGC AGGTGATGCC ATTGGCCTGC AGCCAGATGC CCGTGGTGTG 
GCCACATCCC TGGGGCTTAA CGAGCGGCTC TTTGTTGTCA ACCCACAGGA AGTGCACGAG 
CTGACCCCAC ACCCCGATCA GCTGGGGCCC ACTGTGGGCT CTGCCGAGGG GCTGGACCTG 
GTGAGCACCA AGGACCTGGC GGGCCAGCTC ACGGACCACG ACTGGAGCCT CTTCAACAGC 
ATCCACCAGG TGGAGCTGAT CCACTATGTG CTGGGCCCCC AGCACCTGCG GGACGTCACC 
ACCGCCAACC TGGAGCGCTT CATGCGCCGC TTCAACGAGC TGCAGTACTG GGTGGCCACG 
GAGCTGTGTC TCTGCTCTGT GCCCGGCCTA CGGGCCCAGC TGCTCAGGAA GTTCATCAAA 
CTGGCAGCCC ACCTCAAGGA GCAAAAGAAT CTTAATTCCT TCTTTGCCAT CATGTTTGGT 
CTCAGCAACT CAGCCATCAG CCGCCTGGCC CATACCTGGG AGCGGCTGCC CCACAAAGTC 
CGGAAGCTCT ACTCGGCCCT CGAGAGGCTG CTGGACCCCT CATGGAACCA CCGAGTGTAC 
CGTCTGGCCC TCACCAAGCT CTCCCCTCCC CTCATCCCCT TCATGCCCCT TCTTCTCAAA 
GACATGACCT TCATCCATGA GGGAAATCAC ACATTGGTGG AGAATCTCAT CAACTTTGAG 
AAGATGAGAA TGATGGCCAG AGCTGCACGG ATGCTACACC ACTGCCGAAG CCACAGCAAT 
GTGCCTCTGT CACCGCTCAG AAGCCGAGTC TCCCACCTGC ACGAGGACAG CCAGGCGGTC 
AGGGTTTCCA CGTGCTCGGA GCAGTCCCTG AGCACCCGGA GTCCCGCCAG CACCTGGGCT 
TACGTCCAGC AGCTGAAGGT CATCGACAAC CAGCGGGAAC TGTCCCGCCT CTCCCGGGAG 
CTGGAGCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206449.1
CDS	106..3057
Translation	MVGGVGQNSKEGPDLWYAAAAAGWRPLSAPDRQAPVKVFLSLPRLLAGAQHLRLTSTEDSKEVGWPGEGRWQVGLAVEGDSILGAPPLGGLPDVVPEGTLLNMVLRRMYRSRSCSYQLLLEHQRPSCIQGLRWTPLTSSEESLDFSVSLEQASAERVLRAGKQLHRHLLANCPNLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWAFQDRDTQFYRFPGPEPEPVGVHELEEELVEALALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILGEDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGTGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPTAQLCAALLHHFHAEPAGGSEQERSSYICNKRQQILRLVSQWVTLYGPTLRTDPVATSFLKKLSDLVSRDTRLSNLLREQWPERRRHHRLENGCGNVSPQMKARNVPVWLPSQDEPLPSSNCAIRVGDKVPYDICRPDHSMLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELTPHPDQLGPTVGSAEGLDLVSTKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCSVPGLRAQLLRKFIKLAAHLKEQKNLNSFFAIMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPLIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHSNVPLSPLRSRVSHLHEDSQAVRVSTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	XM_005206392.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206392.3

ORF Insert Sequence:

ATGGTCGGTG GGGTGGGGCA GAATTCCAAA GAAGGGCCTG ATCTCTGGTA CGCCGCCGCC 
GCCGCCGGCT GGAGACCCCT AAGTGCGCCA GACCGGCAGG CCCCAGTGAA GGTCTTCCTG 
TCCCTCCCCC GGCTCCTGGC AGGGGCGCAG CACCTCCGCC TGACCAGTAC AGAGGATTCG 
AAGGAGGTGG GCTGGCCAGG TGAGGGCCGC TGGCAGGTGG GCCTGGCTGT AGAGGGCGAC 
TCGATTCTGG GAGCACCGCC GTTGGGAGGC CTCCCGGACG TGGTGCCGGA GGGGACGCTG 
CTCAACATGG TGCTCAGGAG GATGTATCGG TCCCGAAGCT GCTCCTACCA GCTGCTGCTC 
GAACACCAGC GCCCCAGCTG CATCCAGGGG CTGCGCTGGA CGCCACTCAC CAGCAGCGAG 
GAGTCCCTGG ATTTCAGTGT GAGCCTGGAG CAGGCCTCCG CGGAGCGGGT GCTCAGGGCG 
GGAAAGCAGC TGCATCGACA CCTCCTGGCC AACTGCCCCA ACCTCATCCG AGACAGAAAG 
TACCACCTCC GGCTCCACCG GCAGTGCTGC TCCGGCCGGG AGCTGGTAGA TGGGATCCTG 
GCCCTGGGGC TGGGCGTCCA CTCCCGGAGC CAAGCCGTGG GTATCTGCCA GGTGCTCCTG 
GATGAAGGCG CCCTCTGCCA TGTGAAACAC GACTGGGCCT TCCAGGACCG AGACACCCAG 
TTCTACCGTT TCCCGGGGCC GGAGCCAGAG CCTGTGGGTG TCCATGAGCT AGAGGAAGAG 
CTGGTGGAGG CTCTGGCCCT GTTGTCCCAG CGGGGGCCCG ATGCCCTGCT CACGGTGGCA 
CTTCGAAAGC CCCCTGGTCA GCGCACAGAT GAGGAGCTGG ACCTCATCTT TGAGGAGCTG 
CTGCACATCA AGGCCGTGGC CCACCTCTCC AACTCGGTGA AGCGGGAACT GGCTGCAGTC 
CTGCTCTTTG AACCGCACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGC 
ACCTCGTGGT ACATTATCTG GAAGGGATCG GTCAACGTGG TGACCCATGG CAAGGGCCTG 
GTGACCACCC TGCACGAGGG AGATGACTTC GGACAGCTGG CTCTGGTGAA CGATGCACCT 
CGGGCCGCCA CCATCATCCT GGGAGAAGAC AACTGTCATT TTCTTCGTGT GGACAAGCAG 
GACTTCAACC GTATCATCAA GGATGTGGAA GCAAAGACCA TGAGGCTGGA AGAACATGGC 
AAAGTGGTGT TAGTGCTGGA GAGAACCTCT CAGGGCACTG GCCCTTCTCG TCCCCCAACC 
CCAGGCAGGA ATCGGTATAC AGTGATGTCT GGCACCCCAG AGAAGATCCT AGAACTTCTG 
TTGGAGGCCA TGCGGCCTGA CTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTCCTGA CCCACAGTGT CTTCATGCCC ACTGCCCAGC TCTGTGCTGC CCTCCTGCAC 
CACTTCCACG CGGAGCCCGC CGGGGGCAGC GAGCAGGAGC GCAGCTCCTA CATATGCAAC 
AAGAGGCAGC AGATCCTGCG GCTGGTCAGT CAGTGGGTGA CCCTGTACGG CCCTACGCTC 
CGCACCGACC CTGTGGCCAC CAGCTTCCTC AAGAAACTGT CGGACCTGGT GAGCAGGGAC 
ACCCGGCTTA GCAACCTGCT GCGGGAGCAG TGGCCAGAGA GGCGGCGACA CCACAGGTTG 
GAGAACGGCT GTGGGAACGT GTCTCCGCAG ATGAAGGCCC GGAATGTTCC CGTTTGGCTC 
CCCAGCCAGG ACGAGCCTCT CCCTAGCAGC AACTGTGCCA TCAGAGTTGG GGACAAAGTC 
CCCTATGACA TCTGCCGGCC AGACCACTCC ATGCTAACCC TGCAGCTGCC TGTGACGGCC 
TCAGTGAGAG AGGTGATGGC AGCGTTGGCT CAGGAGGACG GCTGGACTAA GGGGCAGGTG 
CTGGTGAAGG TCAACTCGGC AGGTGATGCC ATTGGCCTGC AGCCAGATGC CCGTGGTGTG 
GCCACATCCC TGGGGCTTAA CGAGCGGCTC TTTGTTGTCA ACCCACAGGA AGTGCACGAG 
CTGACCCCAC ACCCCGATCA GCTGGGGCCC ACTGTGGGCT CTGCCGAGGG GCTGGACCTG 
GTGAGCACCA AGGACCTGGC GGGCCAGCTC ACGGACCACG ACTGGAGCCT CTTCAACAGC 
ATCCACCAGG TGGAGCTGAT CCACTATGTG CTGGGCCCCC AGCACCTGCG GGACGTCACC 
ACCGCCAACC TGGAGCGCTT CATGCGCCGC TTCAACGAGC TGCAGTACTG GGTGGCCACG 
GAGCTGTGTC TCTGCTCTGT GCCCGGCCTA CGGGCCCAGC TGCTCAGGAA GTTCATCAAA 
CTGGCAGCCC ACCTCAAGGA GCAAAAGAAT CTTAATTCCT TCTTTGCCAT CATGTTTGGT 
CTCAGCAACT CAGCCATCAG CCGCCTGGCC CATACCTGGG AGCGGCTGCC CCACAAAGTC 
CGGAAGCTCT ACTCGGCCCT CGAGAGGCTG CTGGACCCCT CATGGAACCA CCGAGTGTAC 
CGTCTGGCCC TCACCAAGCT CTCCCCTCCC CTCATCCCCT TCATGCCCCT TCTTCTCAAA 
GACATGACCT TCATCCATGA GGGAAATCAC ACATTGGTGG AGAATCTCAT CAACTTTGAG 
AAGATGAGAA TGATGGCCAG AGCTGCACGG ATGCTACACC ACTGCCGAAG CCACAGCAAT 
GTGCCTCTGT CACCGCTCAG AAGCCGAGTC TCCCACCTGC ACGAGGACAG CCAGGCGGTC 
AGGGTTTCCA CGTGCTCGGA GCAGTCCCTG AGCACCCGGA GTCCCGCCAG CACCTGGGCT 
TACGTCCAGC AGCTGAAGGT CATCGACAAC CAGCGGGAAC TGTCCCGCCT CTCCCGGGAG 
CTGGAGCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF3 ( XM_005206393.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206450.1 Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa19284
Clone ID Related Accession (Same CDS sequence)	XM_005206393.2 , XM_005206393.1
Accession Version	XM_005206393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA GGAGGATGTA TCGGTCCCGA AGCTGCTCCT ACCAGCTGCT GCTCGAACAC 
CAGCGCCCCA GCTGCATCCA GGGGCTGCGC TGGACGCCAC TCACCAGCAG CGAGGAGTCC 
CTGGATTTCA GTGTGAGCCT GGAGCAGGCC TCCGCGGAGC GGGTGCTCAG GGCGGGAAAG 
CAGCTGCATC GACACCTCCT GGCCAACTGC CCCAACCTCA TCCGAGACAG AAAGTACCAC 
CTCCGGCTCC ACCGGCAGTG CTGCTCCGGC CGGGAGCTGG TAGATGGGAT CCTGGCCCTG 
GGGCTGGGCG TCCACTCCCG GAGCCAAGCC GTGGGTATCT GCCAGGTGCT CCTGGATGAA 
GGCGCCCTCT GCCATGTGAA ACACGACTGG GCCTTCCAGG ACCGAGACAC CCAGTTCTAC 
CGTTTCCCGG GGCCGGAGCC AGAGCCTGTG GGTGTCCATG AGCTAGAGGA AGAGCTGGTG 
GAGGCTCTGG CCCTGTTGTC CCAGCGGGGG CCCGATGCCC TGCTCACGGT GGCACTTCGA 
AAGCCCCCTG GTCAGCGCAC AGATGAGGAG CTGGACCTCA TCTTTGAGGA GCTGCTGCAC 
ATCAAGGCCG TGGCCCACCT CTCCAACTCG GTGAAGCGGG AACTGGCTGC AGTCCTGCTC 
TTTGAACCGC ACAGCAAGGC AGGAACTGTG TTGTTCAGCC AGGGGGACAA GGGCACCTCG 
TGGTACATTA TCTGGAAGGG ATCGGTCAAC GTGGTGACCC ATGGCAAGGG CCTGGTGACC 
ACCCTGCACG AGGGAGATGA CTTCGGACAG CTGGCTCTGG TGAACGATGC ACCTCGGGCC 
GCCACCATCA TCCTGGGAGA AGACAACTGT CATTTTCTTC GTGTGGACAA GCAGGACTTC 
AACCGTATCA TCAAGGATGT GGAAGCAAAG ACCATGAGGC TGGAAGAACA TGGCAAAGTG 
GTGTTAGTGC TGGAGAGAAC CTCTCAGGGC ACTGGCCCTT CTCGTCCCCC AACCCCAGGC 
AGGAATCGGT ATACAGTGAT GTCTGGCACC CCAGAGAAGA TCCTAGAACT TCTGTTGGAG 
GCCATGCGGC CTGACTCCAG TGCTCATGAC CCAACAGAGA CATTCCTCAG TGACTTCCTC 
CTGACCCACA GTGTCTTCAT GCCCACTGCC CAGCTCTGTG CTGCCCTCCT GCACCACTTC 
CACGCGGAGC CCGCCGGGGG CAGCGAGCAG GAGCGCAGCT CCTACATATG CAACAAGAGG 
CAGCAGATCC TGCGGCTGGT CAGTCAGTGG GTGACCCTGT ACGGCCCTAC GCTCCGCACC 
GACCCTGTGG CCACCAGCTT CCTCAAGAAA CTGTCGGACC TGGTGAGCAG GGACACCCGG 
CTTAGCAACC TGCTGCGGGA GCAGTGGCCA GAGAGGCGGC GACACCACAG GTTGGAGAAC 
GGCTGTGGGA ACGTGTCTCC GCAGATGAAG GCCCGGAATG TTCCCGTTTG GCTCCCCAGC 
CAGGACGAGC CTCTCCCTAG CAGCAACTGT GCCATCAGAG TTGGGGACAA AGTCCCCTAT 
GACATCTGCC GGCCAGACCA CTCCATGCTA ACCCTGCAGC TGCCTGTGAC GGCCTCAGTG 
AGAGAGGTGA TGGCAGCGTT GGCTCAGGAG GACGGCTGGA CTAAGGGGCA GGTGCTGGTG 
AAGGTCAACT CGGCAGGTGA TGCCATTGGC CTGCAGCCAG ATGCCCGTGG TGTGGCCACA 
TCCCTGGGGC TTAACGAGCG GCTCTTTGTT GTCAACCCAC AGGAAGTGCA CGAGCTGACC 
CCACACCCCG ATCAGCTGGG GCCCACTGTG GGCTCTGCCG AGGGGCTGGA CCTGGTGAGC 
ACCAAGGACC TGGCGGGCCA GCTCACGGAC CACGACTGGA GCCTCTTCAA CAGCATCCAC 
CAGGTGGAGC TGATCCACTA TGTGCTGGGC CCCCAGCACC TGCGGGACGT CACCACCGCC 
AACCTGGAGC GCTTCATGCG CCGCTTCAAC GAGCTGCAGT ACTGGGTGGC CACGGAGCTG 
TGTCTCTGCT CTGTGCCCGG CCTACGGGCC CAGCTGCTCA GGAAGTTCAT CAAACTGGCA 
GCCCACCTCA AGGAGCAAAA GAATCTTAAT TCCTTCTTTG CCATCATGTT TGGTCTCAGC 
AACTCAGCCA TCAGCCGCCT GGCCCATACC TGGGAGCGGC TGCCCCACAA AGTCCGGAAG 
CTCTACTCGG CCCTCGAGAG GCTGCTGGAC CCCTCATGGA ACCACCGAGT GTACCGTCTG 
GCCCTCACCA AGCTCTCCCC TCCCCTCATC CCCTTCATGC CCCTTCTTCT CAAAGACATG 
ACCTTCATCC ATGAGGGAAA TCACACATTG GTGGAGAATC TCATCAACTT TGAGAAGATG 
AGAATGATGG CCAGAGCTGC ACGGATGCTA CACCACTGCC GAAGCCACAG CAATGTGCCT 
CTGTCACCGC TCAGAAGCCG AGTCTCCCAC CTGCACGAGG ACAGCCAGGC GGTCAGGGTT 
TCCACGTGCT CGGAGCAGTC CCTGAGCACC CGGAGTCCCG CCAGCACCTG GGCTTACGTC 
CAGCAGCTGA AGGTCATCGA CAACCAGCGG GAACTGTCCC GCCTCTCCCG GGAGCTGGAG 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206450.1
CDS	246..2891
Translation	MVLRRMYRSRSCSYQLLLEHQRPSCIQGLRWTPLTSSEESLDFSVSLEQASAERVLRAGKQLHRHLLANCPNLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWAFQDRDTQFYRFPGPEPEPVGVHELEEELVEALALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILGEDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGTGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPTAQLCAALLHHFHAEPAGGSEQERSSYICNKRQQILRLVSQWVTLYGPTLRTDPVATSFLKKLSDLVSRDTRLSNLLREQWPERRRHHRLENGCGNVSPQMKARNVPVWLPSQDEPLPSSNCAIRVGDKVPYDICRPDHSMLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELTPHPDQLGPTVGSAEGLDLVSTKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCSVPGLRAQLLRKFIKLAAHLKEQKNLNSFFAIMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPLIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHSNVPLSPLRSRVSHLHEDSQAVRVSTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	XM_005206393.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206393.2

ORF Insert Sequence:

ATGGTGCTCA GGAGGATGTA TCGGTCCCGA AGCTGCTCCT ACCAGCTGCT GCTCGAACAC 
CAGCGCCCCA GCTGCATCCA GGGGCTGCGC TGGACGCCAC TCACCAGCAG CGAGGAGTCC 
CTGGATTTCA GTGTGAGCCT GGAGCAGGCC TCCGCGGAGC GGGTGCTCAG GGCGGGAAAG 
CAGCTGCATC GACACCTCCT GGCCAACTGC CCCAACCTCA TCCGAGACAG AAAGTACCAC 
CTCCGGCTCC ACCGGCAGTG CTGCTCCGGC CGGGAGCTGG TAGATGGGAT CCTGGCCCTG 
GGGCTGGGCG TCCACTCCCG GAGCCAAGCC GTGGGTATCT GCCAGGTGCT CCTGGATGAA 
GGCGCCCTCT GCCATGTGAA ACACGACTGG GCCTTCCAGG ACCGAGACAC CCAGTTCTAC 
CGTTTCCCGG GGCCGGAGCC AGAGCCTGTG GGTGTCCATG AGCTAGAGGA AGAGCTGGTG 
GAGGCTCTGG CCCTGTTGTC CCAGCGGGGG CCCGATGCCC TGCTCACGGT GGCACTTCGA 
AAGCCCCCTG GTCAGCGCAC AGATGAGGAG CTGGACCTCA TCTTTGAGGA GCTGCTGCAC 
ATCAAGGCCG TGGCCCACCT CTCCAACTCG GTGAAGCGGG AACTGGCTGC AGTCCTGCTC 
TTTGAACCGC ACAGCAAGGC AGGAACTGTG TTGTTCAGCC AGGGGGACAA GGGCACCTCG 
TGGTACATTA TCTGGAAGGG ATCGGTCAAC GTGGTGACCC ATGGCAAGGG CCTGGTGACC 
ACCCTGCACG AGGGAGATGA CTTCGGACAG CTGGCTCTGG TGAACGATGC ACCTCGGGCC 
GCCACCATCA TCCTGGGAGA AGACAACTGT CATTTTCTTC GTGTGGACAA GCAGGACTTC 
AACCGTATCA TCAAGGATGT GGAAGCAAAG ACCATGAGGC TGGAAGAACA TGGCAAAGTG 
GTGTTAGTGC TGGAGAGAAC CTCTCAGGGC ACTGGCCCTT CTCGTCCCCC AACCCCAGGC 
AGGAATCGGT ATACAGTGAT GTCTGGCACC CCAGAGAAGA TCCTAGAACT TCTGTTGGAG 
GCCATGCGGC CTGACTCCAG TGCTCATGAC CCAACAGAGA CATTCCTCAG TGACTTCCTC 
CTGACCCACA GTGTCTTCAT GCCCACTGCC CAGCTCTGTG CTGCCCTCCT GCACCACTTC 
CACGCGGAGC CCGCCGGGGG CAGCGAGCAG GAGCGCAGCT CCTACATATG CAACAAGAGG 
CAGCAGATCC TGCGGCTGGT CAGTCAGTGG GTGACCCTGT ACGGCCCTAC GCTCCGCACC 
GACCCTGTGG CCACCAGCTT CCTCAAGAAA CTGTCGGACC TGGTGAGCAG GGACACCCGG 
CTTAGCAACC TGCTGCGGGA GCAGTGGCCA GAGAGGCGGC GACACCACAG GTTGGAGAAC 
GGCTGTGGGA ACGTGTCTCC GCAGATGAAG GCCCGGAATG TTCCCGTTTG GCTCCCCAGC 
CAGGACGAGC CTCTCCCTAG CAGCAACTGT GCCATCAGAG TTGGGGACAA AGTCCCCTAT 
GACATCTGCC GGCCAGACCA CTCCATGCTA ACCCTGCAGC TGCCTGTGAC GGCCTCAGTG 
AGAGAGGTGA TGGCAGCGTT GGCTCAGGAG GACGGCTGGA CTAAGGGGCA GGTGCTGGTG 
AAGGTCAACT CGGCAGGTGA TGCCATTGGC CTGCAGCCAG ATGCCCGTGG TGTGGCCACA 
TCCCTGGGGC TTAACGAGCG GCTCTTTGTT GTCAACCCAC AGGAAGTGCA CGAGCTGACC 
CCACACCCCG ATCAGCTGGG GCCCACTGTG GGCTCTGCCG AGGGGCTGGA CCTGGTGAGC 
ACCAAGGACC TGGCGGGCCA GCTCACGGAC CACGACTGGA GCCTCTTCAA CAGCATCCAC 
CAGGTGGAGC TGATCCACTA TGTGCTGGGC CCCCAGCACC TGCGGGACGT CACCACCGCC 
AACCTGGAGC GCTTCATGCG CCGCTTCAAC GAGCTGCAGT ACTGGGTGGC CACGGAGCTG 
TGTCTCTGCT CTGTGCCCGG CCTACGGGCC CAGCTGCTCA GGAAGTTCAT CAAACTGGCA 
GCCCACCTCA AGGAGCAAAA GAATCTTAAT TCCTTCTTTG CCATCATGTT TGGTCTCAGC 
AACTCAGCCA TCAGCCGCCT GGCCCATACC TGGGAGCGGC TGCCCCACAA AGTCCGGAAG 
CTCTACTCGG CCCTCGAGAG GCTGCTGGAC CCCTCATGGA ACCACCGAGT GTACCGTCTG 
GCCCTCACCA AGCTCTCCCC TCCCCTCATC CCCTTCATGC CCCTTCTTCT CAAAGACATG 
ACCTTCATCC ATGAGGGAAA TCACACATTG GTGGAGAATC TCATCAACTT TGAGAAGATG 
AGAATGATGG CCAGAGCTGC ACGGATGCTA CACCACTGCC GAAGCCACAG CAATGTGCCT 
CTGTCACCGC TCAGAAGCCG AGTCTCCCAC CTGCACGAGG ACAGCCAGGC GGTCAGGGTT 
TCCACGTGCT CGGAGCAGTC CCTGAGCACC CGGAGTCCCG CCAGCACCTG GGCTTACGTC 
CAGCAGCTGA AGGTCATCGA CAACCAGCGG GAACTGTCCC GCCTCTCCCG GGAGCTGGAG 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF3 ( XM_005206393.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206450.1 Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X3, mRNA.

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CloneID	OBa19284
Clone ID Related Accession (Same CDS sequence)	XM_005206393.2 , XM_005206393.1
Accession Version	XM_005206393.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103921.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA GGAGGATGTA TCGGTCCCGA AGCTGCTCCT ACCAGCTGCT GCTCGAACAC 
CAGCGCCCCA GCTGCATCCA GGGGCTGCGC TGGACGCCAC TCACCAGCAG CGAGGAGTCC 
CTGGATTTCA GTGTGAGCCT GGAGCAGGCC TCCGCGGAGC GGGTGCTCAG GGCGGGAAAG 
CAGCTGCATC GACACCTCCT GGCCAACTGC CCCAACCTCA TCCGAGACAG AAAGTACCAC 
CTCCGGCTCC ACCGGCAGTG CTGCTCCGGC CGGGAGCTGG TAGATGGGAT CCTGGCCCTG 
GGGCTGGGCG TCCACTCCCG GAGCCAAGCC GTGGGTATCT GCCAGGTGCT CCTGGATGAA 
GGCGCCCTCT GCCATGTGAA ACACGACTGG GCCTTCCAGG ACCGAGACAC CCAGTTCTAC 
CGTTTCCCGG GGCCGGAGCC AGAGCCTGTG GGTGTCCATG AGCTAGAGGA AGAGCTGGTG 
GAGGCTCTGG CCCTGTTGTC CCAGCGGGGG CCCGATGCCC TGCTCACGGT GGCACTTCGA 
AAGCCCCCTG GTCAGCGCAC AGATGAGGAG CTGGACCTCA TCTTTGAGGA GCTGCTGCAC 
ATCAAGGCCG TGGCCCACCT CTCCAACTCG GTGAAGCGGG AACTGGCTGC AGTCCTGCTC 
TTTGAACCGC ACAGCAAGGC AGGAACTGTG TTGTTCAGCC AGGGGGACAA GGGCACCTCG 
TGGTACATTA TCTGGAAGGG ATCGGTCAAC GTGGTGACCC ATGGCAAGGG CCTGGTGACC 
ACCCTGCACG AGGGAGATGA CTTCGGACAG CTGGCTCTGG TGAACGATGC ACCTCGGGCC 
GCCACCATCA TCCTGGGAGA AGACAACTGT CATTTTCTTC GTGTGGACAA GCAGGACTTC 
AACCGTATCA TCAAGGATGT GGAAGCAAAG ACCATGAGGC TGGAAGAACA TGGCAAAGTG 
GTGTTAGTGC TGGAGAGAAC CTCTCAGGGC ACTGGCCCTT CTCGTCCCCC AACCCCAGGC 
AGGAATCGGT ATACAGTGAT GTCTGGCACC CCAGAGAAGA TCCTAGAACT TCTGTTGGAG 
GCCATGCGGC CTGACTCCAG TGCTCATGAC CCAACAGAGA CATTCCTCAG TGACTTCCTC 
CTGACCCACA GTGTCTTCAT GCCCACTGCC CAGCTCTGTG CTGCCCTCCT GCACCACTTC 
CACGCGGAGC CCGCCGGGGG CAGCGAGCAG GAGCGCAGCT CCTACATATG CAACAAGAGG 
CAGCAGATCC TGCGGCTGGT CAGTCAGTGG GTGACCCTGT ACGGCCCTAC GCTCCGCACC 
GACCCTGTGG CCACCAGCTT CCTCAAGAAA CTGTCGGACC TGGTGAGCAG GGACACCCGG 
CTTAGCAACC TGCTGCGGGA GCAGTGGCCA GAGAGGCGGC GACACCACAG GTTGGAGAAC 
GGCTGTGGGA ACGTGTCTCC GCAGATGAAG GCCCGGAATG TTCCCGTTTG GCTCCCCAGC 
CAGGACGAGC CTCTCCCTAG CAGCAACTGT GCCATCAGAG TTGGGGACAA AGTCCCCTAT 
GACATCTGCC GGCCAGACCA CTCCATGCTA ACCCTGCAGC TGCCTGTGAC GGCCTCAGTG 
AGAGAGGTGA TGGCAGCGTT GGCTCAGGAG GACGGCTGGA CTAAGGGGCA GGTGCTGGTG 
AAGGTCAACT CGGCAGGTGA TGCCATTGGC CTGCAGCCAG ATGCCCGTGG TGTGGCCACA 
TCCCTGGGGC TTAACGAGCG GCTCTTTGTT GTCAACCCAC AGGAAGTGCA CGAGCTGACC 
CCACACCCCG ATCAGCTGGG GCCCACTGTG GGCTCTGCCG AGGGGCTGGA CCTGGTGAGC 
ACCAAGGACC TGGCGGGCCA GCTCACGGAC CACGACTGGA GCCTCTTCAA CAGCATCCAC 
CAGGTGGAGC TGATCCACTA TGTGCTGGGC CCCCAGCACC TGCGGGACGT CACCACCGCC 
AACCTGGAGC GCTTCATGCG CCGCTTCAAC GAGCTGCAGT ACTGGGTGGC CACGGAGCTG 
TGTCTCTGCT CTGTGCCCGG CCTACGGGCC CAGCTGCTCA GGAAGTTCAT CAAACTGGCA 
GCCCACCTCA AGGAGCAAAA GAATCTTAAT TCCTTCTTTG CCATCATGTT TGGTCTCAGC 
AACTCAGCCA TCAGCCGCCT GGCCCATACC TGGGAGCGGC TGCCCCACAA AGTCCGGAAG 
CTCTACTCGG CCCTCGAGAG GCTGCTGGAC CCCTCATGGA ACCACCGAGT GTACCGTCTG 
GCCCTCACCA AGCTCTCCCC TCCCCTCATC CCCTTCATGC CCCTTCTTCT CAAAGACATG 
ACCTTCATCC ATGAGGGAAA TCACACATTG GTGGAGAATC TCATCAACTT TGAGAAGATG 
AGAATGATGG CCAGAGCTGC ACGGATGCTA CACCACTGCC GAAGCCACAG CAATGTGCCT 
CTGTCACCGC TCAGAAGCCG AGTCTCCCAC CTGCACGAGG ACAGCCAGGC GGTCAGGGTT 
TCCACGTGCT CGGAGCAGTC CCTGAGCACC CGGAGTCCCG CCAGCACCTG GGCTTACGTC 
CAGCAGCTGA AGGTCATCGA CAACCAGCGG GAACTGTCCC GCCTCTCCCG GGAGCTGGAG 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206450.1
CDS	240..2885
Translation	MVLRRMYRSRSCSYQLLLEHQRPSCIQGLRWTPLTSSEESLDFSVSLEQASAERVLRAGKQLHRHLLANCPNLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWAFQDRDTQFYRFPGPEPEPVGVHELEEELVEALALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILGEDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGTGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPTAQLCAALLHHFHAEPAGGSEQERSSYICNKRQQILRLVSQWVTLYGPTLRTDPVATSFLKKLSDLVSRDTRLSNLLREQWPERRRHHRLENGCGNVSPQMKARNVPVWLPSQDEPLPSSNCAIRVGDKVPYDICRPDHSMLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELTPHPDQLGPTVGSAEGLDLVSTKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCSVPGLRAQLLRKFIKLAAHLKEQKNLNSFFAIMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPLIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHSNVPLSPLRSRVSHLHEDSQAVRVSTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	XM_005206393.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 3 (RAPGEF3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206393.1

ORF Insert Sequence:

ATGGTGCTCA GGAGGATGTA TCGGTCCCGA AGCTGCTCCT ACCAGCTGCT GCTCGAACAC 
CAGCGCCCCA GCTGCATCCA GGGGCTGCGC TGGACGCCAC TCACCAGCAG CGAGGAGTCC 
CTGGATTTCA GTGTGAGCCT GGAGCAGGCC TCCGCGGAGC GGGTGCTCAG GGCGGGAAAG 
CAGCTGCATC GACACCTCCT GGCCAACTGC CCCAACCTCA TCCGAGACAG AAAGTACCAC 
CTCCGGCTCC ACCGGCAGTG CTGCTCCGGC CGGGAGCTGG TAGATGGGAT CCTGGCCCTG 
GGGCTGGGCG TCCACTCCCG GAGCCAAGCC GTGGGTATCT GCCAGGTGCT CCTGGATGAA 
GGCGCCCTCT GCCATGTGAA ACACGACTGG GCCTTCCAGG ACCGAGACAC CCAGTTCTAC 
CGTTTCCCGG GGCCGGAGCC AGAGCCTGTG GGTGTCCATG AGCTAGAGGA AGAGCTGGTG 
GAGGCTCTGG CCCTGTTGTC CCAGCGGGGG CCCGATGCCC TGCTCACGGT GGCACTTCGA 
AAGCCCCCTG GTCAGCGCAC AGATGAGGAG CTGGACCTCA TCTTTGAGGA GCTGCTGCAC 
ATCAAGGCCG TGGCCCACCT CTCCAACTCG GTGAAGCGGG AACTGGCTGC AGTCCTGCTC 
TTTGAACCGC ACAGCAAGGC AGGAACTGTG TTGTTCAGCC AGGGGGACAA GGGCACCTCG 
TGGTACATTA TCTGGAAGGG ATCGGTCAAC GTGGTGACCC ATGGCAAGGG CCTGGTGACC 
ACCCTGCACG AGGGAGATGA CTTCGGACAG CTGGCTCTGG TGAACGATGC ACCTCGGGCC 
GCCACCATCA TCCTGGGAGA AGACAACTGT CATTTTCTTC GTGTGGACAA GCAGGACTTC 
AACCGTATCA TCAAGGATGT GGAAGCAAAG ACCATGAGGC TGGAAGAACA TGGCAAAGTG 
GTGTTAGTGC TGGAGAGAAC CTCTCAGGGC ACTGGCCCTT CTCGTCCCCC AACCCCAGGC 
AGGAATCGGT ATACAGTGAT GTCTGGCACC CCAGAGAAGA TCCTAGAACT TCTGTTGGAG 
GCCATGCGGC CTGACTCCAG TGCTCATGAC CCAACAGAGA CATTCCTCAG TGACTTCCTC 
CTGACCCACA GTGTCTTCAT GCCCACTGCC CAGCTCTGTG CTGCCCTCCT GCACCACTTC 
CACGCGGAGC CCGCCGGGGG CAGCGAGCAG GAGCGCAGCT CCTACATATG CAACAAGAGG 
CAGCAGATCC TGCGGCTGGT CAGTCAGTGG GTGACCCTGT ACGGCCCTAC GCTCCGCACC 
GACCCTGTGG CCACCAGCTT CCTCAAGAAA CTGTCGGACC TGGTGAGCAG GGACACCCGG 
CTTAGCAACC TGCTGCGGGA GCAGTGGCCA GAGAGGCGGC GACACCACAG GTTGGAGAAC 
GGCTGTGGGA ACGTGTCTCC GCAGATGAAG GCCCGGAATG TTCCCGTTTG GCTCCCCAGC 
CAGGACGAGC CTCTCCCTAG CAGCAACTGT GCCATCAGAG TTGGGGACAA AGTCCCCTAT 
GACATCTGCC GGCCAGACCA CTCCATGCTA ACCCTGCAGC TGCCTGTGAC GGCCTCAGTG 
AGAGAGGTGA TGGCAGCGTT GGCTCAGGAG GACGGCTGGA CTAAGGGGCA GGTGCTGGTG 
AAGGTCAACT CGGCAGGTGA TGCCATTGGC CTGCAGCCAG ATGCCCGTGG TGTGGCCACA 
TCCCTGGGGC TTAACGAGCG GCTCTTTGTT GTCAACCCAC AGGAAGTGCA CGAGCTGACC 
CCACACCCCG ATCAGCTGGG GCCCACTGTG GGCTCTGCCG AGGGGCTGGA CCTGGTGAGC 
ACCAAGGACC TGGCGGGCCA GCTCACGGAC CACGACTGGA GCCTCTTCAA CAGCATCCAC 
CAGGTGGAGC TGATCCACTA TGTGCTGGGC CCCCAGCACC TGCGGGACGT CACCACCGCC 
AACCTGGAGC GCTTCATGCG CCGCTTCAAC GAGCTGCAGT ACTGGGTGGC CACGGAGCTG 
TGTCTCTGCT CTGTGCCCGG CCTACGGGCC CAGCTGCTCA GGAAGTTCAT CAAACTGGCA 
GCCCACCTCA AGGAGCAAAA GAATCTTAAT TCCTTCTTTG CCATCATGTT TGGTCTCAGC 
AACTCAGCCA TCAGCCGCCT GGCCCATACC TGGGAGCGGC TGCCCCACAA AGTCCGGAAG 
CTCTACTCGG CCCTCGAGAG GCTGCTGGAC CCCTCATGGA ACCACCGAGT GTACCGTCTG 
GCCCTCACCA AGCTCTCCCC TCCCCTCATC CCCTTCATGC CCCTTCTTCT CAAAGACATG 
ACCTTCATCC ATGAGGGAAA TCACACATTG GTGGAGAATC TCATCAACTT TGAGAAGATG 
AGAATGATGG CCAGAGCTGC ACGGATGCTA CACCACTGCC GAAGCCACAG CAATGTGCCT 
CTGTCACCGC TCAGAAGCCG AGTCTCCCAC CTGCACGAGG ACAGCCAGGC GGTCAGGGTT 
TCCACGTGCT CGGAGCAGTC CCTGAGCACC CGGAGTCCCG CCAGCACCTG GGCTTACGTC 
CAGCAGCTGA AGGTCATCGA CAACCAGCGG GAACTGTCCC GCCTCTCCCG GGAGCTGGAG 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prostacyclin regulates bone growth via the Epac/Rap1 pathway.
Endocrinology156(2)499-510(2015 Feb)
Hutchison MR,White PC

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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RAPGEF3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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