MYO6 cDNA ORF clone, Bos taurus(cattle)

The following MYO6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYO6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa237848 XM_024996745.1
Latest version!
Bos taurus myosin VI (MYO6), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa22477 NM_001206072.3
Latest version!
Bos taurus myosin VI (MYO6), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22475 XM_015472774.2
Latest version!
Bos taurus myosin VI (MYO6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22475 XM_005210674.4
Latest version!
Bos taurus myosin VI (MYO6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22475 XM_005210675.3
Latest version!
Bos taurus myosin VI (MYO6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22476 XM_005210676.4
Latest version!
Bos taurus myosin VI (MYO6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22477 XM_005210677.4
Latest version!
Bos taurus myosin VI (MYO6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22478 XM_005210678.4
Latest version!
Bos taurus myosin VI (MYO6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22479 XM_005210679.4
Latest version!
Bos taurus myosin VI (MYO6), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22480 XM_005210680.4
Latest version!
Bos taurus myosin VI (MYO6), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa53036 XM_010808372.3
Latest version!
Bos taurus myosin VI (MYO6), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa22477 NM_001206072.2 Bos taurus myosin VI (MYO6), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22478 XM_005210678.3 Bos taurus myosin VI (MYO6), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa53036 XM_010808372.2 Bos taurus myosin VI (MYO6), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22477 XM_005210677.3 Bos taurus myosin VI (MYO6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22476 XM_005210676.3 Bos taurus myosin VI (MYO6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22475 XM_005210674.3 Bos taurus myosin VI (MYO6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22475 XM_015472774.1 Bos taurus myosin VI (MYO6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22479 XM_005210679.3 Bos taurus myosin VI (MYO6), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22480 XM_005210680.3 Bos taurus myosin VI (MYO6), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa237848
    Clone ID Related Accession (Same CDS sequence) XM_024996745.1
    Accession Version XM_024996745.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AATTAACGGA
    ACAAGACGCA CAATGACACC GGAACAAATG GCGAAAGAGA TGTCAGAAAT TTTGAGTAGA
    GGCCCTGCTG TACAAGCCAC GAAGGCGGCT GCTGGTACCA AGAAACACGA TCTTAGTAAG
    TGGAAATACG CAGAACTCCG TGATACCATC AATACTTCTT GTGATATTGA GCTCCTGGCA
    GCTTGCAGAG AAGAATTTCA TAGGCGACTA AAAGTGTATC ATGCTTGGAA ATCCAAGAAC
    AAGAAGAGAA ATACCGAAAC AGAACAGCGT GCTCCAAAGT CTGTCACTGA TTATGCTCAG
    CAGAACCCGG CAGCCCAGCT TCCTGCCAGG CAGCAGGAGA TCGAGATGAA CCGACAGCAG
    CGTTTCTTCC GCATCCCATT TATCCGCCCC GCTGACCAGT ACAAAGACCC GCAGAATAAG
    AAGAAAGGCT GGTGGTATGC CCATTTTGAT GGGCCGTGGA TCGCCCGGCA GATGGAGCTT
    CATCCTGACA AGCCACCCAT TCTCCTCGTG GCTGGGAAGG ATGACATGGA GATGTGTGAG
    CTGAATCTTG AAGAGACAGG CCTGACTCGA AAGCGCGGTG CTGAGATTCT GCCAAGACAG
    TTCGAAGAAA TCTGGGAGCG CTGTGGAGGC ATCCAGTATC TCCAGAGTGC AATCGAGAGC
    AGGCAGGCCA GACCCACGTA TGCCACGGCC ATGCTGCAGA ATCTGTTAAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852513.1
    CDS271..4104
    Translation

    Target ORF information:

    RefSeq Version XM_024996745.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996745.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AATTAACGGA
    ACAAGACGCA CAATGACACC GGAACAAATG GCGAAAGAGA TGTCAGAAAT TTTGAGTAGA
    GGCCCTGCTG TACAAGCCAC GAAGGCGGCT GCTGGTACCA AGAAACACGA TCTTAGTAAG
    TGGAAATACG CAGAACTCCG TGATACCATC AATACTTCTT GTGATATTGA GCTCCTGGCA
    GCTTGCAGAG AAGAATTTCA TAGGCGACTA AAAGTGTATC ATGCTTGGAA ATCCAAGAAC
    AAGAAGAGAA ATACCGAAAC AGAACAGCGT GCTCCAAAGT CTGTCACTGA TTATGCTCAG
    CAGAACCCGG CAGCCCAGCT TCCTGCCAGG CAGCAGGAGA TCGAGATGAA CCGACAGCAG
    CGTTTCTTCC GCATCCCATT TATCCGCCCC GCTGACCAGT ACAAAGACCC GCAGAATAAG
    AAGAAAGGCT GGTGGTATGC CCATTTTGAT GGGCCGTGGA TCGCCCGGCA GATGGAGCTT
    CATCCTGACA AGCCACCCAT TCTCCTCGTG GCTGGGAAGG ATGACATGGA GATGTGTGAG
    CTGAATCTTG AAGAGACAGG CCTGACTCGA AAGCGCGGTG CTGAGATTCT GCCAAGACAG
    TTCGAAGAAA TCTGGGAGCG CTGTGGAGGC ATCCAGTATC TCCAGAGTGC AATCGAGAGC
    AGGCAGGCCA GACCCACGTA TGCCACGGCC ATGCTGCAGA ATCTGTTAAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22477
    Clone ID Related Accession (Same CDS sequence) NM_001206072.3 , XM_005210677.3 , NM_001206072.2 , XM_005210677.4
    Accession Version NM_001206072.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-23
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000009.1. On May 29, 2018 this sequence version replaced NM_001206072.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AATTAACGGA
    ACAAGACGCA CAATGACACC GGAACAAATG GCGAAAGAGA TGTCAGAAAT TTTGAGTAGA
    GGCCCTGCTG TACAAGCCAC GAAGGCGGCT GCTGGTACCA AGAAACACGA TCTTAGTAAG
    TGGAAATACG CAGAACTCCG TGATACCATC AATACTTCTT GTGATATTGA GCTCCTGGCA
    GCTTGCAGAG AAGAATTTCA TAGGCGACTA AAAGTGTATC ATGCTTGGAA ATCCAAGAAC
    AAGAAGAGAA ATACCGAAAC AGAACAGCGT GCTCCAAAGT CTGTCACTGA TTATGATTTT
    GCACCATTTT TGAACAATTC ACCTCAGCAG AACCCGGCAG CCCAGCTTCC TGCCAGGCAG
    CAGGAGATCG AGATGAACCG ACAGCAGCGT TTCTTCCGCA TCCCATTTAT CCGCCCCGCT
    GACCAGTACA AAGACCCGCA GAATAAGAAG AAAGGCTGGT GGTATGCCCA TTTTGATGGG
    CCGTGGATCG CCCGGCAGAT GGAGCTTCAT CCTGACAAGC CACCCATTCT CCTCGTGGCT
    GGGAAGGATG ACATGGAGAT GTGTGAGCTG AATCTTGAAG AGACAGGCCT GACTCGAAAG
    CGCGGTGCTG AGATTCTGCC AAGACAGTTC GAAGAAATCT GGGAGCGCTG TGGAGGCATC
    CAGTATCTCC AGAGTGCAAT CGAGAGCAGG CAGGCCAGAC CCACGTATGC CACGGCCATG
    CTGCAGAATC TGTTAAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193001.1
    CDS270..4130
    Translation

    Target ORF information:

    RefSeq Version NM_001206072.3
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206072.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AATTAACGGA
    ACAAGACGCA CAATGACACC GGAACAAATG GCGAAAGAGA TGTCAGAAAT TTTGAGTAGA
    GGCCCTGCTG TACAAGCCAC GAAGGCGGCT GCTGGTACCA AGAAACACGA TCTTAGTAAG
    TGGAAATACG CAGAACTCCG TGATACCATC AATACTTCTT GTGATATTGA GCTCCTGGCA
    GCTTGCAGAG AAGAATTTCA TAGGCGACTA AAAGTGTATC ATGCTTGGAA ATCCAAGAAC
    AAGAAGAGAA ATACCGAAAC AGAACAGCGT GCTCCAAAGT CTGTCACTGA TTATGATTTT
    GCACCATTTT TGAACAATTC ACCTCAGCAG AACCCGGCAG CCCAGCTTCC TGCCAGGCAG
    CAGGAGATCG AGATGAACCG ACAGCAGCGT TTCTTCCGCA TCCCATTTAT CCGCCCCGCT
    GACCAGTACA AAGACCCGCA GAATAAGAAG AAAGGCTGGT GGTATGCCCA TTTTGATGGG
    CCGTGGATCG CCCGGCAGAT GGAGCTTCAT CCTGACAAGC CACCCATTCT CCTCGTGGCT
    GGGAAGGATG ACATGGAGAT GTGTGAGCTG AATCTTGAAG AGACAGGCCT GACTCGAAAG
    CGCGGTGCTG AGATTCTGCC AAGACAGTTC GAAGAAATCT GGGAGCGCTG TGGAGGCATC
    CAGTATCTCC AGAGTGCAAT CGAGAGCAGG CAGGCCAGAC CCACGTATGC CACGGCCATG
    CTGCAGAATC TGTTAAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22477
    Clone ID Related Accession (Same CDS sequence) NM_001206072.3 , XM_005210677.3 , NM_001206072.2 , XM_005210677.4
    Accession Version NM_001206072.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02025284.1, DAAA02025285.1 and DAAA02025286.1. On Feb 23, 2016 this sequence version replaced NM_001206072.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822095, SAMN04019650 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AATTAACGGA
    ACAAGACGCA CAATGACACC GGAACAAATG GCGAAAGAGA TGTCAGAAAT TTTGAGTAGA
    GGCCCTGCTG TACAAGCCAC GAAGGCGGCT GCTGGTACCA AGAAACACGA TCTTAGTAAG
    TGGAAATACG CAGAACTCCG TGATACCATC AATACTTCTT GTGATATTGA GCTCCTGGCA
    GCTTGCAGAG AAGAATTTCA TAGGCGACTA AAAGTGTATC ATGCTTGGAA ATCCAAGAAC
    AAGAAGAGAA ATACCGAAAC AGAACAGCGT GCTCCAAAGT CTGTCACTGA TTATGATTTT
    GCACCATTTT TGAACAATTC ACCTCAGCAG AACCCGGCAG CCCAGCTTCC TGCCAGGCAG
    CAGGAGATCG AGATGAACCG ACAGCAGCGT TTCTTCCGCA TCCCATTTAT CCGCCCCGCT
    GACCAGTACA AAGACCCGCA GAATAAGAAG AAAGGCTGGT GGTATGCCCA TTTTGATGGG
    CCGTGGATCG CCCGGCAGAT GGAGCTTCAT CCTGACAAGC CACCCATTCT CCTCGTGGCT
    GGGAAGGATG ACATGGAGAT GTGTGAGCTG AATCTTGAAG AGACAGGCCT GACTCGAAAG
    CGCGGTGCTG AGATTCTGCC AAGACAGTTC GAAGAAATCT GGGAGCGCTG TGGAGGCATC
    CAGTATCTCC AGAGTGCAAT CGAGAGCAGG CAGGCCAGAC CCACGTATGC CACGGCCATG
    CTGCAGAATC TGTTAAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193001.1
    CDS270..4130
    Translation

    Target ORF information:

    RefSeq Version NM_001206072.2
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206072.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AATTAACGGA
    ACAAGACGCA CAATGACACC GGAACAAATG GCGAAAGAGA TGTCAGAAAT TTTGAGTAGA
    GGCCCTGCTG TACAAGCCAC GAAGGCGGCT GCTGGTACCA AGAAACACGA TCTTAGTAAG
    TGGAAATACG CAGAACTCCG TGATACCATC AATACTTCTT GTGATATTGA GCTCCTGGCA
    GCTTGCAGAG AAGAATTTCA TAGGCGACTA AAAGTGTATC ATGCTTGGAA ATCCAAGAAC
    AAGAAGAGAA ATACCGAAAC AGAACAGCGT GCTCCAAAGT CTGTCACTGA TTATGATTTT
    GCACCATTTT TGAACAATTC ACCTCAGCAG AACCCGGCAG CCCAGCTTCC TGCCAGGCAG
    CAGGAGATCG AGATGAACCG ACAGCAGCGT TTCTTCCGCA TCCCATTTAT CCGCCCCGCT
    GACCAGTACA AAGACCCGCA GAATAAGAAG AAAGGCTGGT GGTATGCCCA TTTTGATGGG
    CCGTGGATCG CCCGGCAGAT GGAGCTTCAT CCTGACAAGC CACCCATTCT CCTCGTGGCT
    GGGAAGGATG ACATGGAGAT GTGTGAGCTG AATCTTGAAG AGACAGGCCT GACTCGAAAG
    CGCGGTGCTG AGATTCTGCC AAGACAGTTC GAAGAAATCT GGGAGCGCTG TGGAGGCATC
    CAGTATCTCC AGAGTGCAAT CGAGAGCAGG CAGGCCAGAC CCACGTATGC CACGGCCATG
    CTGCAGAATC TGTTAAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22475
    Clone ID Related Accession (Same CDS sequence) XM_005210675.3 , XM_015472774.1 , XM_005210674.3 , XM_005210674.4 , XM_015472774.2
    Accession Version XM_005210675.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104168.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210675.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCGGA ACAAATGGCG
    AAAGAGATGT CAGAAATTTT GAGTAGAGGC CCTGCTGTAC AAGCCACGAA GGCGGCTGCT
    GGTACCAAGA AACACGATCT TAGTAAGTGG AAATACGCAG AACTCCGTGA TACCATCAAT
    ACTTCTTGTG ATATTGAGCT CCTGGCAGCT TGCAGAGAAG AATTTCATAG GCGACTAAAA
    GTGTATCATG CTTGGAAATC CAAGAACAAG AAGAGAAATA CCGAAACAGA ACAGCGTGCT
    CCAAAGTCTG TCACTGATTA TGATTTTGCA CCATTTTTGA ACAATTCACC TCAGCAGAAC
    CCGGCAGCCC AGCTTCCTGC CAGGCAGCAG GAGATCGAGA TGAACCGACA GCAGCGTTTC
    TTCCGCATCC CATTTATCCG CCCCGCTGAC CAGTACAAAG ACCCGCAGAA TAAGAAGAAA
    GGCTGGTGGT ATGCCCATTT TGATGGGCCG TGGATCGCCC GGCAGATGGA GCTTCATCCT
    GACAAGCCAC CCATTCTCCT CGTGGCTGGG AAGGATGACA TGGAGATGTG TGAGCTGAAT
    CTTGAAGAGA CAGGCCTGAC TCGAAAGCGC GGTGCTGAGA TTCTGCCAAG ACAGTTCGAA
    GAAATCTGGG AGCGCTGTGG AGGCATCCAG TATCTCCAGA GTGCAATCGA GAGCAGGCAG
    GCCAGACCCA CGTATGCCAC GGCCATGCTG CAGAATCTGT TAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210732.1
    CDS300..4187
    Translation

    Target ORF information:

    RefSeq Version XM_005210675.3
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210675.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCGGA ACAAATGGCG
    AAAGAGATGT CAGAAATTTT GAGTAGAGGC CCTGCTGTAC AAGCCACGAA GGCGGCTGCT
    GGTACCAAGA AACACGATCT TAGTAAGTGG AAATACGCAG AACTCCGTGA TACCATCAAT
    ACTTCTTGTG ATATTGAGCT CCTGGCAGCT TGCAGAGAAG AATTTCATAG GCGACTAAAA
    GTGTATCATG CTTGGAAATC CAAGAACAAG AAGAGAAATA CCGAAACAGA ACAGCGTGCT
    CCAAAGTCTG TCACTGATTA TGATTTTGCA CCATTTTTGA ACAATTCACC TCAGCAGAAC
    CCGGCAGCCC AGCTTCCTGC CAGGCAGCAG GAGATCGAGA TGAACCGACA GCAGCGTTTC
    TTCCGCATCC CATTTATCCG CCCCGCTGAC CAGTACAAAG ACCCGCAGAA TAAGAAGAAA
    GGCTGGTGGT ATGCCCATTT TGATGGGCCG TGGATCGCCC GGCAGATGGA GCTTCATCCT
    GACAAGCCAC CCATTCTCCT CGTGGCTGGG AAGGATGACA TGGAGATGTG TGAGCTGAAT
    CTTGAAGAGA CAGGCCTGAC TCGAAAGCGC GGTGCTGAGA TTCTGCCAAG ACAGTTCGAA
    GAAATCTGGG AGCGCTGTGG AGGCATCCAG TATCTCCAGA GTGCAATCGA GAGCAGGCAG
    GCCAGACCCA CGTATGCCAC GGCCATGCTG CAGAATCTGT TAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22475
    Clone ID Related Accession (Same CDS sequence) XM_005210675.3 , XM_015472774.1 , XM_005210674.3 , XM_005210674.4 , XM_015472774.2
    Accession Version XM_015472774.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104168.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCGGA ACAAATGGCG
    AAAGAGATGT CAGAAATTTT GAGTAGAGGC CCTGCTGTAC AAGCCACGAA GGCGGCTGCT
    GGTACCAAGA AACACGATCT TAGTAAGTGG AAATACGCAG AACTCCGTGA TACCATCAAT
    ACTTCTTGTG ATATTGAGCT CCTGGCAGCT TGCAGAGAAG AATTTCATAG GCGACTAAAA
    GTGTATCATG CTTGGAAATC CAAGAACAAG AAGAGAAATA CCGAAACAGA ACAGCGTGCT
    CCAAAGTCTG TCACTGATTA TGATTTTGCA CCATTTTTGA ACAATTCACC TCAGCAGAAC
    CCGGCAGCCC AGCTTCCTGC CAGGCAGCAG GAGATCGAGA TGAACCGACA GCAGCGTTTC
    TTCCGCATCC CATTTATCCG CCCCGCTGAC CAGTACAAAG ACCCGCAGAA TAAGAAGAAA
    GGCTGGTGGT ATGCCCATTT TGATGGGCCG TGGATCGCCC GGCAGATGGA GCTTCATCCT
    GACAAGCCAC CCATTCTCCT CGTGGCTGGG AAGGATGACA TGGAGATGTG TGAGCTGAAT
    CTTGAAGAGA CAGGCCTGAC TCGAAAGCGC GGTGCTGAGA TTCTGCCAAG ACAGTTCGAA
    GAAATCTGGG AGCGCTGTGG AGGCATCCAG TATCTCCAGA GTGCAATCGA GAGCAGGCAG
    GCCAGACCCA CGTATGCCAC GGCCATGCTG CAGAATCTGT TAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328260.1
    CDS45..3932
    Translation

    Target ORF information:

    RefSeq Version XM_015472774.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472774.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCGGA ACAAATGGCG
    AAAGAGATGT CAGAAATTTT GAGTAGAGGC CCTGCTGTAC AAGCCACGAA GGCGGCTGCT
    GGTACCAAGA AACACGATCT TAGTAAGTGG AAATACGCAG AACTCCGTGA TACCATCAAT
    ACTTCTTGTG ATATTGAGCT CCTGGCAGCT TGCAGAGAAG AATTTCATAG GCGACTAAAA
    GTGTATCATG CTTGGAAATC CAAGAACAAG AAGAGAAATA CCGAAACAGA ACAGCGTGCT
    CCAAAGTCTG TCACTGATTA TGATTTTGCA CCATTTTTGA ACAATTCACC TCAGCAGAAC
    CCGGCAGCCC AGCTTCCTGC CAGGCAGCAG GAGATCGAGA TGAACCGACA GCAGCGTTTC
    TTCCGCATCC CATTTATCCG CCCCGCTGAC CAGTACAAAG ACCCGCAGAA TAAGAAGAAA
    GGCTGGTGGT ATGCCCATTT TGATGGGCCG TGGATCGCCC GGCAGATGGA GCTTCATCCT
    GACAAGCCAC CCATTCTCCT CGTGGCTGGG AAGGATGACA TGGAGATGTG TGAGCTGAAT
    CTTGAAGAGA CAGGCCTGAC TCGAAAGCGC GGTGCTGAGA TTCTGCCAAG ACAGTTCGAA
    GAAATCTGGG AGCGCTGTGG AGGCATCCAG TATCTCCAGA GTGCAATCGA GAGCAGGCAG
    GCCAGACCCA CGTATGCCAC GGCCATGCTG CAGAATCTGT TAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22475
    Clone ID Related Accession (Same CDS sequence) XM_005210675.3 , XM_015472774.1 , XM_005210674.3 , XM_005210674.4 , XM_015472774.2
    Accession Version XM_005210674.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104168.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210674.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCGGA ACAAATGGCG
    AAAGAGATGT CAGAAATTTT GAGTAGAGGC CCTGCTGTAC AAGCCACGAA GGCGGCTGCT
    GGTACCAAGA AACACGATCT TAGTAAGTGG AAATACGCAG AACTCCGTGA TACCATCAAT
    ACTTCTTGTG ATATTGAGCT CCTGGCAGCT TGCAGAGAAG AATTTCATAG GCGACTAAAA
    GTGTATCATG CTTGGAAATC CAAGAACAAG AAGAGAAATA CCGAAACAGA ACAGCGTGCT
    CCAAAGTCTG TCACTGATTA TGATTTTGCA CCATTTTTGA ACAATTCACC TCAGCAGAAC
    CCGGCAGCCC AGCTTCCTGC CAGGCAGCAG GAGATCGAGA TGAACCGACA GCAGCGTTTC
    TTCCGCATCC CATTTATCCG CCCCGCTGAC CAGTACAAAG ACCCGCAGAA TAAGAAGAAA
    GGCTGGTGGT ATGCCCATTT TGATGGGCCG TGGATCGCCC GGCAGATGGA GCTTCATCCT
    GACAAGCCAC CCATTCTCCT CGTGGCTGGG AAGGATGACA TGGAGATGTG TGAGCTGAAT
    CTTGAAGAGA CAGGCCTGAC TCGAAAGCGC GGTGCTGAGA TTCTGCCAAG ACAGTTCGAA
    GAAATCTGGG AGCGCTGTGG AGGCATCCAG TATCTCCAGA GTGCAATCGA GAGCAGGCAG
    GCCAGACCCA CGTATGCCAC GGCCATGCTG CAGAATCTGT TAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210731.1
    CDS238..4125
    Translation

    Target ORF information:

    RefSeq Version XM_005210674.3
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210674.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCGGA ACAAATGGCG
    AAAGAGATGT CAGAAATTTT GAGTAGAGGC CCTGCTGTAC AAGCCACGAA GGCGGCTGCT
    GGTACCAAGA AACACGATCT TAGTAAGTGG AAATACGCAG AACTCCGTGA TACCATCAAT
    ACTTCTTGTG ATATTGAGCT CCTGGCAGCT TGCAGAGAAG AATTTCATAG GCGACTAAAA
    GTGTATCATG CTTGGAAATC CAAGAACAAG AAGAGAAATA CCGAAACAGA ACAGCGTGCT
    CCAAAGTCTG TCACTGATTA TGATTTTGCA CCATTTTTGA ACAATTCACC TCAGCAGAAC
    CCGGCAGCCC AGCTTCCTGC CAGGCAGCAG GAGATCGAGA TGAACCGACA GCAGCGTTTC
    TTCCGCATCC CATTTATCCG CCCCGCTGAC CAGTACAAAG ACCCGCAGAA TAAGAAGAAA
    GGCTGGTGGT ATGCCCATTT TGATGGGCCG TGGATCGCCC GGCAGATGGA GCTTCATCCT
    GACAAGCCAC CCATTCTCCT CGTGGCTGGG AAGGATGACA TGGAGATGTG TGAGCTGAAT
    CTTGAAGAGA CAGGCCTGAC TCGAAAGCGC GGTGCTGAGA TTCTGCCAAG ACAGTTCGAA
    GAAATCTGGG AGCGCTGTGG AGGCATCCAG TATCTCCAGA GTGCAATCGA GAGCAGGCAG
    GCCAGACCCA CGTATGCCAC GGCCATGCTG CAGAATCTGT TAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22475
    Clone ID Related Accession (Same CDS sequence) XM_005210675.3 , XM_015472774.1 , XM_005210674.3 , XM_005210674.4 , XM_015472774.2
    Accession Version XM_005210674.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210674.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCGGA ACAAATGGCG
    AAAGAGATGT CAGAAATTTT GAGTAGAGGC CCTGCTGTAC AAGCCACGAA GGCGGCTGCT
    GGTACCAAGA AACACGATCT TAGTAAGTGG AAATACGCAG AACTCCGTGA TACCATCAAT
    ACTTCTTGTG ATATTGAGCT CCTGGCAGCT TGCAGAGAAG AATTTCATAG GCGACTAAAA
    GTGTATCATG CTTGGAAATC CAAGAACAAG AAGAGAAATA CCGAAACAGA ACAGCGTGCT
    CCAAAGTCTG TCACTGATTA TGATTTTGCA CCATTTTTGA ACAATTCACC TCAGCAGAAC
    CCGGCAGCCC AGCTTCCTGC CAGGCAGCAG GAGATCGAGA TGAACCGACA GCAGCGTTTC
    TTCCGCATCC CATTTATCCG CCCCGCTGAC CAGTACAAAG ACCCGCAGAA TAAGAAGAAA
    GGCTGGTGGT ATGCCCATTT TGATGGGCCG TGGATCGCCC GGCAGATGGA GCTTCATCCT
    GACAAGCCAC CCATTCTCCT CGTGGCTGGG AAGGATGACA TGGAGATGTG TGAGCTGAAT
    CTTGAAGAGA CAGGCCTGAC TCGAAAGCGC GGTGCTGAGA TTCTGCCAAG ACAGTTCGAA
    GAAATCTGGG AGCGCTGTGG AGGCATCCAG TATCTCCAGA GTGCAATCGA GAGCAGGCAG
    GCCAGACCCA CGTATGCCAC GGCCATGCTG CAGAATCTGT TAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210731.1
    CDS271..4158
    Translation

    Target ORF information:

    RefSeq Version XM_005210674.4
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210674.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCGGA ACAAATGGCG
    AAAGAGATGT CAGAAATTTT GAGTAGAGGC CCTGCTGTAC AAGCCACGAA GGCGGCTGCT
    GGTACCAAGA AACACGATCT TAGTAAGTGG AAATACGCAG AACTCCGTGA TACCATCAAT
    ACTTCTTGTG ATATTGAGCT CCTGGCAGCT TGCAGAGAAG AATTTCATAG GCGACTAAAA
    GTGTATCATG CTTGGAAATC CAAGAACAAG AAGAGAAATA CCGAAACAGA ACAGCGTGCT
    CCAAAGTCTG TCACTGATTA TGATTTTGCA CCATTTTTGA ACAATTCACC TCAGCAGAAC
    CCGGCAGCCC AGCTTCCTGC CAGGCAGCAG GAGATCGAGA TGAACCGACA GCAGCGTTTC
    TTCCGCATCC CATTTATCCG CCCCGCTGAC CAGTACAAAG ACCCGCAGAA TAAGAAGAAA
    GGCTGGTGGT ATGCCCATTT TGATGGGCCG TGGATCGCCC GGCAGATGGA GCTTCATCCT
    GACAAGCCAC CCATTCTCCT CGTGGCTGGG AAGGATGACA TGGAGATGTG TGAGCTGAAT
    CTTGAAGAGA CAGGCCTGAC TCGAAAGCGC GGTGCTGAGA TTCTGCCAAG ACAGTTCGAA
    GAAATCTGGG AGCGCTGTGG AGGCATCCAG TATCTCCAGA GTGCAATCGA GAGCAGGCAG
    GCCAGACCCA CGTATGCCAC GGCCATGCTG CAGAATCTGT TAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22475
    Clone ID Related Accession (Same CDS sequence) XM_005210675.3 , XM_015472774.1 , XM_005210674.3 , XM_005210674.4 , XM_015472774.2
    Accession Version XM_015472774.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCGGA ACAAATGGCG
    AAAGAGATGT CAGAAATTTT GAGTAGAGGC CCTGCTGTAC AAGCCACGAA GGCGGCTGCT
    GGTACCAAGA AACACGATCT TAGTAAGTGG AAATACGCAG AACTCCGTGA TACCATCAAT
    ACTTCTTGTG ATATTGAGCT CCTGGCAGCT TGCAGAGAAG AATTTCATAG GCGACTAAAA
    GTGTATCATG CTTGGAAATC CAAGAACAAG AAGAGAAATA CCGAAACAGA ACAGCGTGCT
    CCAAAGTCTG TCACTGATTA TGATTTTGCA CCATTTTTGA ACAATTCACC TCAGCAGAAC
    CCGGCAGCCC AGCTTCCTGC CAGGCAGCAG GAGATCGAGA TGAACCGACA GCAGCGTTTC
    TTCCGCATCC CATTTATCCG CCCCGCTGAC CAGTACAAAG ACCCGCAGAA TAAGAAGAAA
    GGCTGGTGGT ATGCCCATTT TGATGGGCCG TGGATCGCCC GGCAGATGGA GCTTCATCCT
    GACAAGCCAC CCATTCTCCT CGTGGCTGGG AAGGATGACA TGGAGATGTG TGAGCTGAAT
    CTTGAAGAGA CAGGCCTGAC TCGAAAGCGC GGTGCTGAGA TTCTGCCAAG ACAGTTCGAA
    GAAATCTGGG AGCGCTGTGG AGGCATCCAG TATCTCCAGA GTGCAATCGA GAGCAGGCAG
    GCCAGACCCA CGTATGCCAC GGCCATGCTG CAGAATCTGT TAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328260.1
    CDS403..4290
    Translation

    Target ORF information:

    RefSeq Version XM_015472774.2
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472774.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCGGA ACAAATGGCG
    AAAGAGATGT CAGAAATTTT GAGTAGAGGC CCTGCTGTAC AAGCCACGAA GGCGGCTGCT
    GGTACCAAGA AACACGATCT TAGTAAGTGG AAATACGCAG AACTCCGTGA TACCATCAAT
    ACTTCTTGTG ATATTGAGCT CCTGGCAGCT TGCAGAGAAG AATTTCATAG GCGACTAAAA
    GTGTATCATG CTTGGAAATC CAAGAACAAG AAGAGAAATA CCGAAACAGA ACAGCGTGCT
    CCAAAGTCTG TCACTGATTA TGATTTTGCA CCATTTTTGA ACAATTCACC TCAGCAGAAC
    CCGGCAGCCC AGCTTCCTGC CAGGCAGCAG GAGATCGAGA TGAACCGACA GCAGCGTTTC
    TTCCGCATCC CATTTATCCG CCCCGCTGAC CAGTACAAAG ACCCGCAGAA TAAGAAGAAA
    GGCTGGTGGT ATGCCCATTT TGATGGGCCG TGGATCGCCC GGCAGATGGA GCTTCATCCT
    GACAAGCCAC CCATTCTCCT CGTGGCTGGG AAGGATGACA TGGAGATGTG TGAGCTGAAT
    CTTGAAGAGA CAGGCCTGAC TCGAAAGCGC GGTGCTGAGA TTCTGCCAAG ACAGTTCGAA
    GAAATCTGGG AGCGCTGTGG AGGCATCCAG TATCTCCAGA GTGCAATCGA GAGCAGGCAG
    GCCAGACCCA CGTATGCCAC GGCCATGCTG CAGAATCTGT TAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22476
    Clone ID Related Accession (Same CDS sequence) XM_005210676.4 , XM_005210676.3
    Accession Version XM_005210676.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210676.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCGGA ACAAATGGCG
    AAAGAGATGT CAGAAATTTT GAGTAGAGGC CCTGCTGTAC AAGCCACGAA GGCGGCTGCT
    GGTACCAAGA AACACGATCT TAGTAAGTGG AAATACGCAG AACTCCGTGA TACCATCAAT
    ACTTCTTGTG ATATTGAGCT CCTGGCAGCT TGCAGAGAAG AATTTCATAG GCGACTAAAA
    GTGTATCATG CTTGGAAATC CAAGAACAAG AAGAGAAATA CCGAAACAGA ACAGCGTGCT
    CCAAAGTCTG TCACTGATTA TGCTCAGCAG AACCCGGCAG CCCAGCTTCC TGCCAGGCAG
    CAGGAGATCG AGATGAACCG ACAGCAGCGT TTCTTCCGCA TCCCATTTAT CCGCCCCGCT
    GACCAGTACA AAGACCCGCA GAATAAGAAG AAAGGCTGGT GGTATGCCCA TTTTGATGGG
    CCGTGGATCG CCCGGCAGAT GGAGCTTCAT CCTGACAAGC CACCCATTCT CCTCGTGGCT
    GGGAAGGATG ACATGGAGAT GTGTGAGCTG AATCTTGAAG AGACAGGCCT GACTCGAAAG
    CGCGGTGCTG AGATTCTGCC AAGACAGTTC GAAGAAATCT GGGAGCGCTG TGGAGGCATC
    CAGTATCTCC AGAGTGCAAT CGAGAGCAGG CAGGCCAGAC CCACGTATGC CACGGCCATG
    CTGCAGAATC TGTTAAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210733.1
    CDS271..4131
    Translation

    Target ORF information:

    RefSeq Version XM_005210676.4
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210676.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCGGA ACAAATGGCG
    AAAGAGATGT CAGAAATTTT GAGTAGAGGC CCTGCTGTAC AAGCCACGAA GGCGGCTGCT
    GGTACCAAGA AACACGATCT TAGTAAGTGG AAATACGCAG AACTCCGTGA TACCATCAAT
    ACTTCTTGTG ATATTGAGCT CCTGGCAGCT TGCAGAGAAG AATTTCATAG GCGACTAAAA
    GTGTATCATG CTTGGAAATC CAAGAACAAG AAGAGAAATA CCGAAACAGA ACAGCGTGCT
    CCAAAGTCTG TCACTGATTA TGCTCAGCAG AACCCGGCAG CCCAGCTTCC TGCCAGGCAG
    CAGGAGATCG AGATGAACCG ACAGCAGCGT TTCTTCCGCA TCCCATTTAT CCGCCCCGCT
    GACCAGTACA AAGACCCGCA GAATAAGAAG AAAGGCTGGT GGTATGCCCA TTTTGATGGG
    CCGTGGATCG CCCGGCAGAT GGAGCTTCAT CCTGACAAGC CACCCATTCT CCTCGTGGCT
    GGGAAGGATG ACATGGAGAT GTGTGAGCTG AATCTTGAAG AGACAGGCCT GACTCGAAAG
    CGCGGTGCTG AGATTCTGCC AAGACAGTTC GAAGAAATCT GGGAGCGCTG TGGAGGCATC
    CAGTATCTCC AGAGTGCAAT CGAGAGCAGG CAGGCCAGAC CCACGTATGC CACGGCCATG
    CTGCAGAATC TGTTAAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22476
    Clone ID Related Accession (Same CDS sequence) XM_005210676.4 , XM_005210676.3
    Accession Version XM_005210676.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104168.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210676.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCGGA ACAAATGGCG
    AAAGAGATGT CAGAAATTTT GAGTAGAGGC CCTGCTGTAC AAGCCACGAA GGCGGCTGCT
    GGTACCAAGA AACACGATCT TAGTAAGTGG AAATACGCAG AACTCCGTGA TACCATCAAT
    ACTTCTTGTG ATATTGAGCT CCTGGCAGCT TGCAGAGAAG AATTTCATAG GCGACTAAAA
    GTGTATCATG CTTGGAAATC CAAGAACAAG AAGAGAAATA CCGAAACAGA ACAGCGTGCT
    CCAAAGTCTG TCACTGATTA TGCTCAGCAG AACCCGGCAG CCCAGCTTCC TGCCAGGCAG
    CAGGAGATCG AGATGAACCG ACAGCAGCGT TTCTTCCGCA TCCCATTTAT CCGCCCCGCT
    GACCAGTACA AAGACCCGCA GAATAAGAAG AAAGGCTGGT GGTATGCCCA TTTTGATGGG
    CCGTGGATCG CCCGGCAGAT GGAGCTTCAT CCTGACAAGC CACCCATTCT CCTCGTGGCT
    GGGAAGGATG ACATGGAGAT GTGTGAGCTG AATCTTGAAG AGACAGGCCT GACTCGAAAG
    CGCGGTGCTG AGATTCTGCC AAGACAGTTC GAAGAAATCT GGGAGCGCTG TGGAGGCATC
    CAGTATCTCC AGAGTGCAAT CGAGAGCAGG CAGGCCAGAC CCACGTATGC CACGGCCATG
    CTGCAGAATC TGTTAAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210733.1
    CDS237..4097
    Translation

    Target ORF information:

    RefSeq Version XM_005210676.3
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210676.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCGGA ACAAATGGCG
    AAAGAGATGT CAGAAATTTT GAGTAGAGGC CCTGCTGTAC AAGCCACGAA GGCGGCTGCT
    GGTACCAAGA AACACGATCT TAGTAAGTGG AAATACGCAG AACTCCGTGA TACCATCAAT
    ACTTCTTGTG ATATTGAGCT CCTGGCAGCT TGCAGAGAAG AATTTCATAG GCGACTAAAA
    GTGTATCATG CTTGGAAATC CAAGAACAAG AAGAGAAATA CCGAAACAGA ACAGCGTGCT
    CCAAAGTCTG TCACTGATTA TGCTCAGCAG AACCCGGCAG CCCAGCTTCC TGCCAGGCAG
    CAGGAGATCG AGATGAACCG ACAGCAGCGT TTCTTCCGCA TCCCATTTAT CCGCCCCGCT
    GACCAGTACA AAGACCCGCA GAATAAGAAG AAAGGCTGGT GGTATGCCCA TTTTGATGGG
    CCGTGGATCG CCCGGCAGAT GGAGCTTCAT CCTGACAAGC CACCCATTCT CCTCGTGGCT
    GGGAAGGATG ACATGGAGAT GTGTGAGCTG AATCTTGAAG AGACAGGCCT GACTCGAAAG
    CGCGGTGCTG AGATTCTGCC AAGACAGTTC GAAGAAATCT GGGAGCGCTG TGGAGGCATC
    CAGTATCTCC AGAGTGCAAT CGAGAGCAGG CAGGCCAGAC CCACGTATGC CACGGCCATG
    CTGCAGAATC TGTTAAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22477
    Clone ID Related Accession (Same CDS sequence) NM_001206072.3 , XM_005210677.3 , NM_001206072.2 , XM_005210677.4
    Accession Version XM_005210677.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104168.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210677.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AATTAACGGA
    ACAAGACGCA CAATGACACC GGAACAAATG GCGAAAGAGA TGTCAGAAAT TTTGAGTAGA
    GGCCCTGCTG TACAAGCCAC GAAGGCGGCT GCTGGTACCA AGAAACACGA TCTTAGTAAG
    TGGAAATACG CAGAACTCCG TGATACCATC AATACTTCTT GTGATATTGA GCTCCTGGCA
    GCTTGCAGAG AAGAATTTCA TAGGCGACTA AAAGTGTATC ATGCTTGGAA ATCCAAGAAC
    AAGAAGAGAA ATACCGAAAC AGAACAGCGT GCTCCAAAGT CTGTCACTGA TTATGATTTT
    GCACCATTTT TGAACAATTC ACCTCAGCAG AACCCGGCAG CCCAGCTTCC TGCCAGGCAG
    CAGGAGATCG AGATGAACCG ACAGCAGCGT TTCTTCCGCA TCCCATTTAT CCGCCCCGCT
    GACCAGTACA AAGACCCGCA GAATAAGAAG AAAGGCTGGT GGTATGCCCA TTTTGATGGG
    CCGTGGATCG CCCGGCAGAT GGAGCTTCAT CCTGACAAGC CACCCATTCT CCTCGTGGCT
    GGGAAGGATG ACATGGAGAT GTGTGAGCTG AATCTTGAAG AGACAGGCCT GACTCGAAAG
    CGCGGTGCTG AGATTCTGCC AAGACAGTTC GAAGAAATCT GGGAGCGCTG TGGAGGCATC
    CAGTATCTCC AGAGTGCAAT CGAGAGCAGG CAGGCCAGAC CCACGTATGC CACGGCCATG
    CTGCAGAATC TGTTAAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210734.1
    CDS238..4098
    Translation

    Target ORF information:

    RefSeq Version XM_005210677.3
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210677.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AATTAACGGA
    ACAAGACGCA CAATGACACC GGAACAAATG GCGAAAGAGA TGTCAGAAAT TTTGAGTAGA
    GGCCCTGCTG TACAAGCCAC GAAGGCGGCT GCTGGTACCA AGAAACACGA TCTTAGTAAG
    TGGAAATACG CAGAACTCCG TGATACCATC AATACTTCTT GTGATATTGA GCTCCTGGCA
    GCTTGCAGAG AAGAATTTCA TAGGCGACTA AAAGTGTATC ATGCTTGGAA ATCCAAGAAC
    AAGAAGAGAA ATACCGAAAC AGAACAGCGT GCTCCAAAGT CTGTCACTGA TTATGATTTT
    GCACCATTTT TGAACAATTC ACCTCAGCAG AACCCGGCAG CCCAGCTTCC TGCCAGGCAG
    CAGGAGATCG AGATGAACCG ACAGCAGCGT TTCTTCCGCA TCCCATTTAT CCGCCCCGCT
    GACCAGTACA AAGACCCGCA GAATAAGAAG AAAGGCTGGT GGTATGCCCA TTTTGATGGG
    CCGTGGATCG CCCGGCAGAT GGAGCTTCAT CCTGACAAGC CACCCATTCT CCTCGTGGCT
    GGGAAGGATG ACATGGAGAT GTGTGAGCTG AATCTTGAAG AGACAGGCCT GACTCGAAAG
    CGCGGTGCTG AGATTCTGCC AAGACAGTTC GAAGAAATCT GGGAGCGCTG TGGAGGCATC
    CAGTATCTCC AGAGTGCAAT CGAGAGCAGG CAGGCCAGAC CCACGTATGC CACGGCCATG
    CTGCAGAATC TGTTAAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22477
    Clone ID Related Accession (Same CDS sequence) NM_001206072.3 , XM_005210677.3 , NM_001206072.2 , XM_005210677.4
    Accession Version XM_005210677.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210677.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AATTAACGGA
    ACAAGACGCA CAATGACACC GGAACAAATG GCGAAAGAGA TGTCAGAAAT TTTGAGTAGA
    GGCCCTGCTG TACAAGCCAC GAAGGCGGCT GCTGGTACCA AGAAACACGA TCTTAGTAAG
    TGGAAATACG CAGAACTCCG TGATACCATC AATACTTCTT GTGATATTGA GCTCCTGGCA
    GCTTGCAGAG AAGAATTTCA TAGGCGACTA AAAGTGTATC ATGCTTGGAA ATCCAAGAAC
    AAGAAGAGAA ATACCGAAAC AGAACAGCGT GCTCCAAAGT CTGTCACTGA TTATGATTTT
    GCACCATTTT TGAACAATTC ACCTCAGCAG AACCCGGCAG CCCAGCTTCC TGCCAGGCAG
    CAGGAGATCG AGATGAACCG ACAGCAGCGT TTCTTCCGCA TCCCATTTAT CCGCCCCGCT
    GACCAGTACA AAGACCCGCA GAATAAGAAG AAAGGCTGGT GGTATGCCCA TTTTGATGGG
    CCGTGGATCG CCCGGCAGAT GGAGCTTCAT CCTGACAAGC CACCCATTCT CCTCGTGGCT
    GGGAAGGATG ACATGGAGAT GTGTGAGCTG AATCTTGAAG AGACAGGCCT GACTCGAAAG
    CGCGGTGCTG AGATTCTGCC AAGACAGTTC GAAGAAATCT GGGAGCGCTG TGGAGGCATC
    CAGTATCTCC AGAGTGCAAT CGAGAGCAGG CAGGCCAGAC CCACGTATGC CACGGCCATG
    CTGCAGAATC TGTTAAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210734.1
    CDS271..4131
    Translation

    Target ORF information:

    RefSeq Version XM_005210677.4
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210677.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AATTAACGGA
    ACAAGACGCA CAATGACACC GGAACAAATG GCGAAAGAGA TGTCAGAAAT TTTGAGTAGA
    GGCCCTGCTG TACAAGCCAC GAAGGCGGCT GCTGGTACCA AGAAACACGA TCTTAGTAAG
    TGGAAATACG CAGAACTCCG TGATACCATC AATACTTCTT GTGATATTGA GCTCCTGGCA
    GCTTGCAGAG AAGAATTTCA TAGGCGACTA AAAGTGTATC ATGCTTGGAA ATCCAAGAAC
    AAGAAGAGAA ATACCGAAAC AGAACAGCGT GCTCCAAAGT CTGTCACTGA TTATGATTTT
    GCACCATTTT TGAACAATTC ACCTCAGCAG AACCCGGCAG CCCAGCTTCC TGCCAGGCAG
    CAGGAGATCG AGATGAACCG ACAGCAGCGT TTCTTCCGCA TCCCATTTAT CCGCCCCGCT
    GACCAGTACA AAGACCCGCA GAATAAGAAG AAAGGCTGGT GGTATGCCCA TTTTGATGGG
    CCGTGGATCG CCCGGCAGAT GGAGCTTCAT CCTGACAAGC CACCCATTCT CCTCGTGGCT
    GGGAAGGATG ACATGGAGAT GTGTGAGCTG AATCTTGAAG AGACAGGCCT GACTCGAAAG
    CGCGGTGCTG AGATTCTGCC AAGACAGTTC GAAGAAATCT GGGAGCGCTG TGGAGGCATC
    CAGTATCTCC AGAGTGCAAT CGAGAGCAGG CAGGCCAGAC CCACGTATGC CACGGCCATG
    CTGCAGAATC TGTTAAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22478
    Clone ID Related Accession (Same CDS sequence) XM_005210678.4 , XM_005210678.3
    Accession Version XM_005210678.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210678.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCAGG CCCTGCTGTA
    CAAGCCACGA AGGCGGCTGC TGGTACCAAG AAACACGATC TTAGTAAGTG GAAATACGCA
    GAACTCCGTG ATACCATCAA TACTTCTTGT GATATTGAGC TCCTGGCAGC TTGCAGAGAA
    GAATTTCATA GGCGACTAAA AGTGTATCAT GCTTGGAAAT CCAAGAACAA GAAGAGAAAT
    ACCGAAACAG AACAGCGTGC TCCAAAGTCT GTCACTGATT ATGATTTTGC ACCATTTTTG
    AACAATTCAC CTCAGCAGAA CCCGGCAGCC CAGCTTCCTG CCAGGCAGCA GGAGATCGAG
    ATGAACCGAC AGCAGCGTTT CTTCCGCATC CCATTTATCC GCCCCGCTGA CCAGTACAAA
    GACCCGCAGA ATAAGAAGAA AGGCTGGTGG TATGCCCATT TTGATGGGCC GTGGATCGCC
    CGGCAGATGG AGCTTCATCC TGACAAGCCA CCCATTCTCC TCGTGGCTGG GAAGGATGAC
    ATGGAGATGT GTGAGCTGAA TCTTGAAGAG ACAGGCCTGA CTCGAAAGCG CGGTGCTGAG
    ATTCTGCCAA GACAGTTCGA AGAAATCTGG GAGCGCTGTG GAGGCATCCA GTATCTCCAG
    AGTGCAATCG AGAGCAGGCA GGCCAGACCC ACGTATGCCA CGGCCATGCT GCAGAATCTG
    TTAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210735.1
    CDS271..4119
    Translation

    Target ORF information:

    RefSeq Version XM_005210678.4
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210678.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCAGG CCCTGCTGTA
    CAAGCCACGA AGGCGGCTGC TGGTACCAAG AAACACGATC TTAGTAAGTG GAAATACGCA
    GAACTCCGTG ATACCATCAA TACTTCTTGT GATATTGAGC TCCTGGCAGC TTGCAGAGAA
    GAATTTCATA GGCGACTAAA AGTGTATCAT GCTTGGAAAT CCAAGAACAA GAAGAGAAAT
    ACCGAAACAG AACAGCGTGC TCCAAAGTCT GTCACTGATT ATGATTTTGC ACCATTTTTG
    AACAATTCAC CTCAGCAGAA CCCGGCAGCC CAGCTTCCTG CCAGGCAGCA GGAGATCGAG
    ATGAACCGAC AGCAGCGTTT CTTCCGCATC CCATTTATCC GCCCCGCTGA CCAGTACAAA
    GACCCGCAGA ATAAGAAGAA AGGCTGGTGG TATGCCCATT TTGATGGGCC GTGGATCGCC
    CGGCAGATGG AGCTTCATCC TGACAAGCCA CCCATTCTCC TCGTGGCTGG GAAGGATGAC
    ATGGAGATGT GTGAGCTGAA TCTTGAAGAG ACAGGCCTGA CTCGAAAGCG CGGTGCTGAG
    ATTCTGCCAA GACAGTTCGA AGAAATCTGG GAGCGCTGTG GAGGCATCCA GTATCTCCAG
    AGTGCAATCG AGAGCAGGCA GGCCAGACCC ACGTATGCCA CGGCCATGCT GCAGAATCTG
    TTAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22478
    Clone ID Related Accession (Same CDS sequence) XM_005210678.4 , XM_005210678.3
    Accession Version XM_005210678.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104168.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210678.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCAGG CCCTGCTGTA
    CAAGCCACGA AGGCGGCTGC TGGTACCAAG AAACACGATC TTAGTAAGTG GAAATACGCA
    GAACTCCGTG ATACCATCAA TACTTCTTGT GATATTGAGC TCCTGGCAGC TTGCAGAGAA
    GAATTTCATA GGCGACTAAA AGTGTATCAT GCTTGGAAAT CCAAGAACAA GAAGAGAAAT
    ACCGAAACAG AACAGCGTGC TCCAAAGTCT GTCACTGATT ATGATTTTGC ACCATTTTTG
    AACAATTCAC CTCAGCAGAA CCCGGCAGCC CAGCTTCCTG CCAGGCAGCA GGAGATCGAG
    ATGAACCGAC AGCAGCGTTT CTTCCGCATC CCATTTATCC GCCCCGCTGA CCAGTACAAA
    GACCCGCAGA ATAAGAAGAA AGGCTGGTGG TATGCCCATT TTGATGGGCC GTGGATCGCC
    CGGCAGATGG AGCTTCATCC TGACAAGCCA CCCATTCTCC TCGTGGCTGG GAAGGATGAC
    ATGGAGATGT GTGAGCTGAA TCTTGAAGAG ACAGGCCTGA CTCGAAAGCG CGGTGCTGAG
    ATTCTGCCAA GACAGTTCGA AGAAATCTGG GAGCGCTGTG GAGGCATCCA GTATCTCCAG
    AGTGCAATCG AGAGCAGGCA GGCCAGACCC ACGTATGCCA CGGCCATGCT GCAGAATCTG
    TTAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210735.1
    CDS238..4086
    Translation

    Target ORF information:

    RefSeq Version XM_005210678.3
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210678.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG CTTGGATTTC
    CATCCAGTAA CTTCTAAAAT TAACGGAACA AGACGCACAA TGACACCAGG CCCTGCTGTA
    CAAGCCACGA AGGCGGCTGC TGGTACCAAG AAACACGATC TTAGTAAGTG GAAATACGCA
    GAACTCCGTG ATACCATCAA TACTTCTTGT GATATTGAGC TCCTGGCAGC TTGCAGAGAA
    GAATTTCATA GGCGACTAAA AGTGTATCAT GCTTGGAAAT CCAAGAACAA GAAGAGAAAT
    ACCGAAACAG AACAGCGTGC TCCAAAGTCT GTCACTGATT ATGATTTTGC ACCATTTTTG
    AACAATTCAC CTCAGCAGAA CCCGGCAGCC CAGCTTCCTG CCAGGCAGCA GGAGATCGAG
    ATGAACCGAC AGCAGCGTTT CTTCCGCATC CCATTTATCC GCCCCGCTGA CCAGTACAAA
    GACCCGCAGA ATAAGAAGAA AGGCTGGTGG TATGCCCATT TTGATGGGCC GTGGATCGCC
    CGGCAGATGG AGCTTCATCC TGACAAGCCA CCCATTCTCC TCGTGGCTGG GAAGGATGAC
    ATGGAGATGT GTGAGCTGAA TCTTGAAGAG ACAGGCCTGA CTCGAAAGCG CGGTGCTGAG
    ATTCTGCCAA GACAGTTCGA AGAAATCTGG GAGCGCTGTG GAGGCATCCA GTATCTCCAG
    AGTGCAATCG AGAGCAGGCA GGCCAGACCC ACGTATGCCA CGGCCATGCT GCAGAATCTG
    TTAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22479
    Clone ID Related Accession (Same CDS sequence) XM_005210679.3 , XM_005210679.4
    Accession Version XM_005210679.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104168.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210679.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AATTAACGGA
    ACAAGACGCA CAATGACACC AGGCCCTGCT GTACAAGCCA CGAAGGCGGC TGCTGGTACC
    AAGAAACACG ATCTTAGTAA GTGGAAATAC GCAGAACTCC GTGATACCAT CAATACTTCT
    TGTGATATTG AGCTCCTGGC AGCTTGCAGA GAAGAATTTC ATAGGCGACT AAAAGTGTAT
    CATGCTTGGA AATCCAAGAA CAAGAAGAGA AATACCGAAA CAGAACAGCG TGCTCCAAAG
    TCTGTCACTG ATTATGATTT TGCACCATTT TTGAACAATT CACCTCAGCA GAACCCGGCA
    GCCCAGCTTC CTGCCAGGCA GCAGGAGATC GAGATGAACC GACAGCAGCG TTTCTTCCGC
    ATCCCATTTA TCCGCCCCGC TGACCAGTAC AAAGACCCGC AGAATAAGAA GAAAGGCTGG
    TGGTATGCCC ATTTTGATGG GCCGTGGATC GCCCGGCAGA TGGAGCTTCA TCCTGACAAG
    CCACCCATTC TCCTCGTGGC TGGGAAGGAT GACATGGAGA TGTGTGAGCT GAATCTTGAA
    GAGACAGGCC TGACTCGAAA GCGCGGTGCT GAGATTCTGC CAAGACAGTT CGAAGAAATC
    TGGGAGCGCT GTGGAGGCAT CCAGTATCTC CAGAGTGCAA TCGAGAGCAG GCAGGCCAGA
    CCCACGTATG CCACGGCCAT GCTGCAGAAT CTGTTAAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210736.1
    CDS238..4059
    Translation

    Target ORF information:

    RefSeq Version XM_005210679.3
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210679.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AATTAACGGA
    ACAAGACGCA CAATGACACC AGGCCCTGCT GTACAAGCCA CGAAGGCGGC TGCTGGTACC
    AAGAAACACG ATCTTAGTAA GTGGAAATAC GCAGAACTCC GTGATACCAT CAATACTTCT
    TGTGATATTG AGCTCCTGGC AGCTTGCAGA GAAGAATTTC ATAGGCGACT AAAAGTGTAT
    CATGCTTGGA AATCCAAGAA CAAGAAGAGA AATACCGAAA CAGAACAGCG TGCTCCAAAG
    TCTGTCACTG ATTATGATTT TGCACCATTT TTGAACAATT CACCTCAGCA GAACCCGGCA
    GCCCAGCTTC CTGCCAGGCA GCAGGAGATC GAGATGAACC GACAGCAGCG TTTCTTCCGC
    ATCCCATTTA TCCGCCCCGC TGACCAGTAC AAAGACCCGC AGAATAAGAA GAAAGGCTGG
    TGGTATGCCC ATTTTGATGG GCCGTGGATC GCCCGGCAGA TGGAGCTTCA TCCTGACAAG
    CCACCCATTC TCCTCGTGGC TGGGAAGGAT GACATGGAGA TGTGTGAGCT GAATCTTGAA
    GAGACAGGCC TGACTCGAAA GCGCGGTGCT GAGATTCTGC CAAGACAGTT CGAAGAAATC
    TGGGAGCGCT GTGGAGGCAT CCAGTATCTC CAGAGTGCAA TCGAGAGCAG GCAGGCCAGA
    CCCACGTATG CCACGGCCAT GCTGCAGAAT CTGTTAAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22479
    Clone ID Related Accession (Same CDS sequence) XM_005210679.3 , XM_005210679.4
    Accession Version XM_005210679.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210679.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AATTAACGGA
    ACAAGACGCA CAATGACACC AGGCCCTGCT GTACAAGCCA CGAAGGCGGC TGCTGGTACC
    AAGAAACACG ATCTTAGTAA GTGGAAATAC GCAGAACTCC GTGATACCAT CAATACTTCT
    TGTGATATTG AGCTCCTGGC AGCTTGCAGA GAAGAATTTC ATAGGCGACT AAAAGTGTAT
    CATGCTTGGA AATCCAAGAA CAAGAAGAGA AATACCGAAA CAGAACAGCG TGCTCCAAAG
    TCTGTCACTG ATTATGATTT TGCACCATTT TTGAACAATT CACCTCAGCA GAACCCGGCA
    GCCCAGCTTC CTGCCAGGCA GCAGGAGATC GAGATGAACC GACAGCAGCG TTTCTTCCGC
    ATCCCATTTA TCCGCCCCGC TGACCAGTAC AAAGACCCGC AGAATAAGAA GAAAGGCTGG
    TGGTATGCCC ATTTTGATGG GCCGTGGATC GCCCGGCAGA TGGAGCTTCA TCCTGACAAG
    CCACCCATTC TCCTCGTGGC TGGGAAGGAT GACATGGAGA TGTGTGAGCT GAATCTTGAA
    GAGACAGGCC TGACTCGAAA GCGCGGTGCT GAGATTCTGC CAAGACAGTT CGAAGAAATC
    TGGGAGCGCT GTGGAGGCAT CCAGTATCTC CAGAGTGCAA TCGAGAGCAG GCAGGCCAGA
    CCCACGTATG CCACGGCCAT GCTGCAGAAT CTGTTAAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210736.1
    CDS271..4092
    Translation

    Target ORF information:

    RefSeq Version XM_005210679.4
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210679.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AATTAACGGA
    ACAAGACGCA CAATGACACC AGGCCCTGCT GTACAAGCCA CGAAGGCGGC TGCTGGTACC
    AAGAAACACG ATCTTAGTAA GTGGAAATAC GCAGAACTCC GTGATACCAT CAATACTTCT
    TGTGATATTG AGCTCCTGGC AGCTTGCAGA GAAGAATTTC ATAGGCGACT AAAAGTGTAT
    CATGCTTGGA AATCCAAGAA CAAGAAGAGA AATACCGAAA CAGAACAGCG TGCTCCAAAG
    TCTGTCACTG ATTATGATTT TGCACCATTT TTGAACAATT CACCTCAGCA GAACCCGGCA
    GCCCAGCTTC CTGCCAGGCA GCAGGAGATC GAGATGAACC GACAGCAGCG TTTCTTCCGC
    ATCCCATTTA TCCGCCCCGC TGACCAGTAC AAAGACCCGC AGAATAAGAA GAAAGGCTGG
    TGGTATGCCC ATTTTGATGG GCCGTGGATC GCCCGGCAGA TGGAGCTTCA TCCTGACAAG
    CCACCCATTC TCCTCGTGGC TGGGAAGGAT GACATGGAGA TGTGTGAGCT GAATCTTGAA
    GAGACAGGCC TGACTCGAAA GCGCGGTGCT GAGATTCTGC CAAGACAGTT CGAAGAAATC
    TGGGAGCGCT GTGGAGGCAT CCAGTATCTC CAGAGTGCAA TCGAGAGCAG GCAGGCCAGA
    CCCACGTATG CCACGGCCAT GCTGCAGAAT CTGTTAAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22480
    Clone ID Related Accession (Same CDS sequence) XM_005210680.3 , XM_005210680.4
    Accession Version XM_005210680.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104168.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210680.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AGGCCCTGCT
    GTACAAGCCA CGAAGGCGGC TGCTGGTACC AAGAAACACG ATCTTAGTAA GTGGAAATAC
    GCAGAACTCC GTGATACCAT CAATACTTCT TGTGATATTG AGCTCCTGGC AGCTTGCAGA
    GAAGAATTTC ATAGGCGACT AAAAGTGTAT CATGCTTGGA AATCCAAGAA CAAGAAGAGA
    AATACCGAAA CAGAACAGCG TGCTCCAAAG TCTGTCACTG ATTATGATTT TGCACCATTT
    TTGAACAATT CACCTCAGCA GAACCCGGCA GCCCAGCTTC CTGCCAGGCA GCAGGAGATC
    GAGATGAACC GACAGCAGCG TTTCTTCCGC ATCCCATTTA TCCGCCCCGC TGACCAGTAC
    AAAGACCCGC AGAATAAGAA GAAAGGCTGG TGGTATGCCC ATTTTGATGG GCCGTGGATC
    GCCCGGCAGA TGGAGCTTCA TCCTGACAAG CCACCCATTC TCCTCGTGGC TGGGAAGGAT
    GACATGGAGA TGTGTGAGCT GAATCTTGAA GAGACAGGCC TGACTCGAAA GCGCGGTGCT
    GAGATTCTGC CAAGACAGTT CGAAGAAATC TGGGAGCGCT GTGGAGGCAT CCAGTATCTC
    CAGAGTGCAA TCGAGAGCAG GCAGGCCAGA CCCACGTATG CCACGGCCAT GCTGCAGAAT
    CTGTTAAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210737.1
    CDS237..4028
    Translation

    Target ORF information:

    RefSeq Version XM_005210680.3
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210680.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AGGCCCTGCT
    GTACAAGCCA CGAAGGCGGC TGCTGGTACC AAGAAACACG ATCTTAGTAA GTGGAAATAC
    GCAGAACTCC GTGATACCAT CAATACTTCT TGTGATATTG AGCTCCTGGC AGCTTGCAGA
    GAAGAATTTC ATAGGCGACT AAAAGTGTAT CATGCTTGGA AATCCAAGAA CAAGAAGAGA
    AATACCGAAA CAGAACAGCG TGCTCCAAAG TCTGTCACTG ATTATGATTT TGCACCATTT
    TTGAACAATT CACCTCAGCA GAACCCGGCA GCCCAGCTTC CTGCCAGGCA GCAGGAGATC
    GAGATGAACC GACAGCAGCG TTTCTTCCGC ATCCCATTTA TCCGCCCCGC TGACCAGTAC
    AAAGACCCGC AGAATAAGAA GAAAGGCTGG TGGTATGCCC ATTTTGATGG GCCGTGGATC
    GCCCGGCAGA TGGAGCTTCA TCCTGACAAG CCACCCATTC TCCTCGTGGC TGGGAAGGAT
    GACATGGAGA TGTGTGAGCT GAATCTTGAA GAGACAGGCC TGACTCGAAA GCGCGGTGCT
    GAGATTCTGC CAAGACAGTT CGAAGAAATC TGGGAGCGCT GTGGAGGCAT CCAGTATCTC
    CAGAGTGCAA TCGAGAGCAG GCAGGCCAGA CCCACGTATG CCACGGCCAT GCTGCAGAAT
    CTGTTAAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22480
    Clone ID Related Accession (Same CDS sequence) XM_005210680.3 , XM_005210680.4
    Accession Version XM_005210680.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210680.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AGGCCCTGCT
    GTACAAGCCA CGAAGGCGGC TGCTGGTACC AAGAAACACG ATCTTAGTAA GTGGAAATAC
    GCAGAACTCC GTGATACCAT CAATACTTCT TGTGATATTG AGCTCCTGGC AGCTTGCAGA
    GAAGAATTTC ATAGGCGACT AAAAGTGTAT CATGCTTGGA AATCCAAGAA CAAGAAGAGA
    AATACCGAAA CAGAACAGCG TGCTCCAAAG TCTGTCACTG ATTATGATTT TGCACCATTT
    TTGAACAATT CACCTCAGCA GAACCCGGCA GCCCAGCTTC CTGCCAGGCA GCAGGAGATC
    GAGATGAACC GACAGCAGCG TTTCTTCCGC ATCCCATTTA TCCGCCCCGC TGACCAGTAC
    AAAGACCCGC AGAATAAGAA GAAAGGCTGG TGGTATGCCC ATTTTGATGG GCCGTGGATC
    GCCCGGCAGA TGGAGCTTCA TCCTGACAAG CCACCCATTC TCCTCGTGGC TGGGAAGGAT
    GACATGGAGA TGTGTGAGCT GAATCTTGAA GAGACAGGCC TGACTCGAAA GCGCGGTGCT
    GAGATTCTGC CAAGACAGTT CGAAGAAATC TGGGAGCGCT GTGGAGGCAT CCAGTATCTC
    CAGAGTGCAA TCGAGAGCAG GCAGGCCAGA CCCACGTATG CCACGGCCAT GCTGCAGAAT
    CTGTTAAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210737.1
    CDS271..4062
    Translation

    Target ORF information:

    RefSeq Version XM_005210680.4
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210680.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AGGCCCTGCT
    GTACAAGCCA CGAAGGCGGC TGCTGGTACC AAGAAACACG ATCTTAGTAA GTGGAAATAC
    GCAGAACTCC GTGATACCAT CAATACTTCT TGTGATATTG AGCTCCTGGC AGCTTGCAGA
    GAAGAATTTC ATAGGCGACT AAAAGTGTAT CATGCTTGGA AATCCAAGAA CAAGAAGAGA
    AATACCGAAA CAGAACAGCG TGCTCCAAAG TCTGTCACTG ATTATGATTT TGCACCATTT
    TTGAACAATT CACCTCAGCA GAACCCGGCA GCCCAGCTTC CTGCCAGGCA GCAGGAGATC
    GAGATGAACC GACAGCAGCG TTTCTTCCGC ATCCCATTTA TCCGCCCCGC TGACCAGTAC
    AAAGACCCGC AGAATAAGAA GAAAGGCTGG TGGTATGCCC ATTTTGATGG GCCGTGGATC
    GCCCGGCAGA TGGAGCTTCA TCCTGACAAG CCACCCATTC TCCTCGTGGC TGGGAAGGAT
    GACATGGAGA TGTGTGAGCT GAATCTTGAA GAGACAGGCC TGACTCGAAA GCGCGGTGCT
    GAGATTCTGC CAAGACAGTT CGAAGAAATC TGGGAGCGCT GTGGAGGCAT CCAGTATCTC
    CAGAGTGCAA TCGAGAGCAG GCAGGCCAGA CCCACGTATG CCACGGCCAT GCTGCAGAAT
    CTGTTAAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53036
    Clone ID Related Accession (Same CDS sequence) XM_010808372.3 , XM_010808372.2
    Accession Version XM_010808372.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010808372.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AGGCCCTGCT
    GTACAAGCCA CGAAGGCGGC TGCTGGTACC AAGAAACACG ATCTTAGTAA GTGGAAATAC
    GCAGAACTCC GTGATACCAT CAATACTTCT TGTGATATTG AGCTCCTGGC AGCTTGCAGA
    GAAGAATTTC ATAGGCGACT AAAAGTGTAT CATGCTTGGA AATCCAAGAA CAAGAAGAGA
    AATACCGAAA CAGAACAGCG TGCTCCAAAG TCTGTCACTG ATTATGCTCA GCAGAACCCG
    GCAGCCCAGC TTCCTGCCAG GCAGCAGGAG ATCGAGATGA ACCGACAGCA GCGTTTCTTC
    CGCATCCCAT TTATCCGCCC CGCTGACCAG TACAAAGACC CGCAGAATAA GAAGAAAGGC
    TGGTGGTATG CCCATTTTGA TGGGCCGTGG ATCGCCCGGC AGATGGAGCT TCATCCTGAC
    AAGCCACCCA TTCTCCTCGT GGCTGGGAAG GATGACATGG AGATGTGTGA GCTGAATCTT
    GAAGAGACAG GCCTGACTCG AAAGCGCGGT GCTGAGATTC TGCCAAGACA GTTCGAAGAA
    ATCTGGGAGC GCTGTGGAGG CATCCAGTAT CTCCAGAGTG CAATCGAGAG CAGGCAGGCC
    AGACCCACGT ATGCCACGGC CATGCTGCAG AATCTGTTAA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806674.1
    CDS271..4035
    Translation

    Target ORF information:

    RefSeq Version XM_010808372.3
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808372.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AGGCCCTGCT
    GTACAAGCCA CGAAGGCGGC TGCTGGTACC AAGAAACACG ATCTTAGTAA GTGGAAATAC
    GCAGAACTCC GTGATACCAT CAATACTTCT TGTGATATTG AGCTCCTGGC AGCTTGCAGA
    GAAGAATTTC ATAGGCGACT AAAAGTGTAT CATGCTTGGA AATCCAAGAA CAAGAAGAGA
    AATACCGAAA CAGAACAGCG TGCTCCAAAG TCTGTCACTG ATTATGCTCA GCAGAACCCG
    GCAGCCCAGC TTCCTGCCAG GCAGCAGGAG ATCGAGATGA ACCGACAGCA GCGTTTCTTC
    CGCATCCCAT TTATCCGCCC CGCTGACCAG TACAAAGACC CGCAGAATAA GAAGAAAGGC
    TGGTGGTATG CCCATTTTGA TGGGCCGTGG ATCGCCCGGC AGATGGAGCT TCATCCTGAC
    AAGCCACCCA TTCTCCTCGT GGCTGGGAAG GATGACATGG AGATGTGTGA GCTGAATCTT
    GAAGAGACAG GCCTGACTCG AAAGCGCGGT GCTGAGATTC TGCCAAGACA GTTCGAAGAA
    ATCTGGGAGC GCTGTGGAGG CATCCAGTAT CTCCAGAGTG CAATCGAGAG CAGGCAGGCC
    AGACCCACGT ATGCCACGGC CATGCTGCAG AATCTGTTAA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53036
    Clone ID Related Accession (Same CDS sequence) XM_010808372.3 , XM_010808372.2
    Accession Version XM_010808372.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product unconventional myosin-VI isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104168.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808372.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AGGCCCTGCT
    GTACAAGCCA CGAAGGCGGC TGCTGGTACC AAGAAACACG ATCTTAGTAA GTGGAAATAC
    GCAGAACTCC GTGATACCAT CAATACTTCT TGTGATATTG AGCTCCTGGC AGCTTGCAGA
    GAAGAATTTC ATAGGCGACT AAAAGTGTAT CATGCTTGGA AATCCAAGAA CAAGAAGAGA
    AATACCGAAA CAGAACAGCG TGCTCCAAAG TCTGTCACTG ATTATGCTCA GCAGAACCCG
    GCAGCCCAGC TTCCTGCCAG GCAGCAGGAG ATCGAGATGA ACCGACAGCA GCGTTTCTTC
    CGCATCCCAT TTATCCGCCC CGCTGACCAG TACAAAGACC CGCAGAATAA GAAGAAAGGC
    TGGTGGTATG CCCATTTTGA TGGGCCGTGG ATCGCCCGGC AGATGGAGCT TCATCCTGAC
    AAGCCACCCA TTCTCCTCGT GGCTGGGAAG GATGACATGG AGATGTGTGA GCTGAATCTT
    GAAGAGACAG GCCTGACTCG AAAGCGCGGT GCTGAGATTC TGCCAAGACA GTTCGAAGAA
    ATCTGGGAGC GCTGTGGAGG CATCCAGTAT CTCCAGAGTG CAATCGAGAG CAGGCAGGCC
    AGACCCACGT ATGCCACGGC CATGCTGCAG AATCTGTTAA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806674.1
    CDS237..4001
    Translation

    Target ORF information:

    RefSeq Version XM_010808372.2
    Organism Bos taurus(cattle)
    Definition Bos taurus myosin VI (MYO6), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808372.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGGACG GAAGGCCGGT GTGGGCCCCG CACCCCACAG AGGGGTTTCA GATGGGCAAC 
    ATCGTGGATA TTGGCCCCGA CAGCTTAACC ATTGAGCCCC TGGGCCAAAA AGGCAAGACA
    TTTTTGGCAC TCATAAACCA AGTGTTTCCT GCAGAAGAGG ACAGTAAAAA AGATGTGGAA
    GATAACTGTT CATTAATGTA TTTAAATGAA GCCACACTAC TCCATAATAT CAAAGTTCGA
    TACAGTAAAG ACAGAATTTA TACCTATGTG GCCAACATTC TGATTGCAGT GAACCCATAC
    TTTGACATAC CTAAAATCTA TTCTTCAGAT TCAATAAAGT CGTACCAAGG AAAATCTCTT
    GGGACGATGC CACCCCATGT GTTTGCAATC GCTGATAAGG CTTTTCGAGA CATGAAGGTG
    CTCAAGATGA GTCAGTCCAT CATTGTATCT GGAGAATCAG GAGCTGGCAA AACGGAAAAT
    ACAAAATTTG TTCTAAGGTA TCTGACTGAA TCCTATGGAT CCGGTCAAGA CATTGATGAT
    AGAATTGTTG AAGCTAACCC ACTCTTAGAA GCTTTTGGAA ATGCAAAGAC TGTTCGCAAC
    AATAATAGTA GTCGATTTGG GAAATTTGTA GAAATACATT TCAATGAAAA GAGTTCAGTT
    GTTGGAGGAT TTGTTTCACA TTATCTTCTA GAGAAATCTA GGATCTGTGT TCAAGGCAAA
    GAGGAAAGGA ATTATCATAT CTTTTATAGG TTATGTGCTG GTGCTTCTGA AGATATTAGG
    GAAAGACTTC ATTTGAGCTC TCCAGATAAT TTTCGGTATT TAAACCGAGG CTGCACTAGA
    TACTTTGCTA ACAAAGAAAC TGACAAACAG ATTTTACAGA ACCGAAAAAC TCCTGAGCAT
    CTTAAGGCAG GTTCCTTGAA AGATCCTTTG TTGGATGACC ATGGAGATTT TGTTAGAATG
    TGCACAGCCA TGAAAAAGAT TGGCTTGGAT GATGAAGAAA AGCTTGATCT GTTCCGGGTA
    GTAGCTGGGG TCCTGCACCT TGGGAATATT GATTTTGAGG AAGCTGGCAG CACTTCAGGT
    GGTTGTAATC TGAAGAATAA ATCTACTCAG TCATTGGAAT ATTGTGCTGA ATTACTGGGT
    CTGGATCAAG ATGATCTTCG TGTAAGTTTA ACTACAAGAG TCATGCTGAC AACAGCAGGG
    GGCACCAAAG GAACAGTGAT AAAGGTACCC CTGAAAGTGG AACAGGCAAA CAATGCCCGG
    GATGCCCTGG CAAAGACCGT CTATAGCCAT CTTTTTGATC ATGTGGTCAA CAGAGTGAAC
    CAGTGTTTTC CTTTTGAAAC ATCATCCTAT TTTATTGGAG TCCTGGATAT TGCTGGGTTT
    GAGTACTTTG AACATAACAG TTTTGAACAA TTTTGTATCA ACTATTGCAA TGAGAAGCTT
    CAACAATTTT TTAATGAAAG GATTCTGAAG GAGGAACAAG AACTCTATCA AAAGGAAGGT
    TTAGGTGTGA ATGAAGTGCA TTATGTGGAT AATCAGGACT GTATAGATTT AATTGAAGCA
    AAATTAATGG GAATATTAGA TATTTTGGAT GAAGAAAATC GCCTTCCCCA GCCAAGTGAT
    CAGCACTTCA CATCTGCAGT TCACCAGAAG CACAAAGACC ATTTCCGACT CTCTATTCCT
    AGAAAGTCTA AGCTGGCAGT TCACAGGAAC ATCCGAGATG ACGAAGGCTT CATCGTCCGG
    CATTTTGCCG GGGCAGTGTG CTATGAAACA ACGCAGTTTG TGGAAAAAAA TAATGACGCC
    TTACATATGT CTCTTGAGTC CTTAATATGT GAATCCAGGG ATAAATTTAT CCGGGAATTA
    TTTGAGTCAT CCACAAATAA CAACAAAGAC ACCAAACAAA AAGCGGGAAA ACTTAGCTTC
    ATCAGTGTGG GAAACAAGTT TAAGACACAG TTAAATTTGC TTCTGGATAA ACTTCGAAGT
    ACAGGAGCAA GCTTTATTCG CTGTATCAAG CCTAATTTAA AGATGACAAG CCATGACTTT
    GAAGGAGCTC AAATTCTGTC TCAGCTTCAG TGTTCAGGTA TGGTGTCTGT CTTGGACTTG
    ATGCAAGGTG GTTTCCCATC ACGAGCTTCA TTTCACGAAC TCTACAACAT GTATAAAAAG
    TATATGCCAG ATAAACTTGC AAGATTAGAT CCAAGACTAT TTTGTAAGGC TCTTTTTAAA
    GCTTTGGGCT TAAATGAAGT TGACTACAAG TTTGGGTTAA CTAAAGTATT TTTTAGACCT
    GGCAAGTTTG CAGAATTTGA TCAGATTATG AAGTCTGACC CTGACCACTT AGCACAGCTG
    GTGAAGAGGG TCAACCACTG GCTAATCTGC AGTCGCTGGA AGAAGGTCCA GTGGTGCTCG
    CTGTCCGTCA TCAAATTGAA AAACAAAATA AAATATCGAG CTGAAGCCTG CATTAAAATG
    CAGAAAACTA TTCGAATGTG GCTTTGCAAA AGGAGACACA AACCTCGCAT TGATGGCCTG
    GTCAAGGTGG GCACACTGAA AAAACGGCTC GATAAATTTA ATGAAGTAGT AAGTGCATTG
    AAAGATGGAA AAGCAGAGAT GAATAAACAG GTCAAGGACC TTGAAATTTC TATTGATGCT
    TTAATGGCCA AAATTAAGTC CACGATGATG ACAAGGGAAC AAATACAGAA AGAATATGAT
    GCTCTAGTTA AAAGCTCAGA GGTCCTTCTC AGCGCATTAC AGAAAAAAAA GCAGCAGGAA
    GAAGAAGCGG AAAGGCTGAG GCGCATTCAA GAAGAAATGG AAAAAGAAAG AAAAAGACGT
    GAAGAAGATG AGCAGCGTCG AAGAAAGGAG GAGGAGGAAA GGCGGATGAA ACTTGAGATG
    GAAGCAAAGA GAAAACAAGA AGAAGAAGAA AGAAAGAAAA GGGAAGATGA TGAAAAACGT
    ATTCAGGCAG AGGTGGAGGC GCAGCTGGCC CGGCAGCGGG AGGAGGAGTC CCAGCAGCAG
    GCAGTTCTGG AGCAGGAGCG CCGGGACCGA GAGCTGGCGC TACGTATCGC CCGGAGCGAG
    GCCGAGCTCA TCATCGACGA GGCACAGGCC GACCCTGCGG CGCTGCGCAG AGGCCCTGCT
    GTACAAGCCA CGAAGGCGGC TGCTGGTACC AAGAAACACG ATCTTAGTAA GTGGAAATAC
    GCAGAACTCC GTGATACCAT CAATACTTCT TGTGATATTG AGCTCCTGGC AGCTTGCAGA
    GAAGAATTTC ATAGGCGACT AAAAGTGTAT CATGCTTGGA AATCCAAGAA CAAGAAGAGA
    AATACCGAAA CAGAACAGCG TGCTCCAAAG TCTGTCACTG ATTATGCTCA GCAGAACCCG
    GCAGCCCAGC TTCCTGCCAG GCAGCAGGAG ATCGAGATGA ACCGACAGCA GCGTTTCTTC
    CGCATCCCAT TTATCCGCCC CGCTGACCAG TACAAAGACC CGCAGAATAA GAAGAAAGGC
    TGGTGGTATG CCCATTTTGA TGGGCCGTGG ATCGCCCGGC AGATGGAGCT TCATCCTGAC
    AAGCCACCCA TTCTCCTCGT GGCTGGGAAG GATGACATGG AGATGTGTGA GCTGAATCTT
    GAAGAGACAG GCCTGACTCG AAAGCGCGGT GCTGAGATTC TGCCAAGACA GTTCGAAGAA
    ATCTGGGAGC GCTGTGGAGG CATCCAGTAT CTCCAGAGTG CAATCGAGAG CAGGCAGGCC
    AGACCCACGT ATGCCACGGC CATGCTGCAG AATCTGTTAA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.