KIF13A gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015469029.1	XP_015324515.1	kinesin-like protein KIF13A isoform X5
XM_015469030.1	XP_015324516.1	kinesin-like protein KIF13A isoform X6
XM_010818550.2	XP_010816852.1	kinesin-like protein KIF13A isoform X4
XM_010818551.2	XP_010816853.1	kinesin-like protein KIF13A isoform X5
XM_010818556.2	XP_010816858.1	kinesin-like protein KIF13A isoform X7
XM_010818549.3	XP_010816851.2	kinesin-like protein KIF13A isoform X1
XM_024983945.1	XP_024839713.1	kinesin-like protein KIF13A isoform X4
XM_010818549.2	XP_010816851.1	kinesin-like protein KIF13A isoform X3
XM_015469028.1	XP_015324514.1	kinesin-like protein KIF13A isoform X4
XM_015469025.1	XP_015324511.1	kinesin-like protein KIF13A isoform X1
XM_015469027.1	XP_015324513.1	kinesin-like protein KIF13A isoform X3
XM_015469031.1	XP_015324517.1	kinesin-like protein KIF13A isoform X7
XM_010818548.2	XP_010816850.1	kinesin-like protein KIF13A isoform X2
XM_010818552.2	XP_010816854.1	kinesin-like protein KIF13A isoform X6
XM_010818547.2	XP_010816849.1	kinesin-like protein KIF13A isoform X1
XM_010818552.3	XP_010816854.2	kinesin-like protein KIF13A isoform X3
XM_010818556.3	XP_010816858.2	kinesin-like protein KIF13A isoform X7
XM_024983947.1	XP_024839715.1	kinesin-like protein KIF13A isoform X6
XM_015469026.1	XP_015324512.1	kinesin-like protein KIF13A isoform X2
XM_010818550.3	XP_010816852.2	kinesin-like protein KIF13A isoform X2
XM_024983946.1	XP_024839714.1	kinesin-like protein KIF13A isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa229715	XM_010818556.3 Latest version!	Bos taurus kinesin family member 13A (KIF13A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa229709	XM_010818549.3 Latest version!	Bos taurus kinesin family member 13A (KIF13A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229710	XM_010818550.3 Latest version!	Bos taurus kinesin family member 13A (KIF13A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229711	XM_010818552.3 Latest version!	Bos taurus kinesin family member 13A (KIF13A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229712	XM_024983945.1 Latest version!	Bos taurus kinesin family member 13A (KIF13A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229713	XM_024983946.1 Latest version!	Bos taurus kinesin family member 13A (KIF13A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229714	XM_024983947.1 Latest version!	Bos taurus kinesin family member 13A (KIF13A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa32275	XM_015469028.1 Latest version!	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa32275	XM_010818550.2 Latest version!	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa32276	XM_010818552.2 Latest version!	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa32276	XM_015469030.1 Latest version!	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52954	XM_015469025.1 Latest version!	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52954	XM_010818547.2 Latest version!	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52955	XM_015469026.1 Latest version!	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52955	XM_010818548.2 Latest version!	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52956	XM_010818549.2 Latest version!	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52956	XM_015469027.1 Latest version!	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52957	XM_010818551.2 Latest version!	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52957	XM_015469029.1 Latest version!	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa52961	XM_010818556.2 Latest version!	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa52961	XM_015469031.1 Latest version!	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

KIF13A ( XM_010818556.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816858.2 Bos taurus kinesin family member 13A (KIF13A), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa229715
Clone ID Related Accession (Same CDS sequence)	XM_010818556.3
Accession Version	XM_010818556.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818556.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGATA CCAAGGTAAA AGTTGCCGTC CGGGTCCGGC CCCTGAACCG ACGAGAACTG 
GAACTCAACA CCAAGTGCGT GGTGGAGATG GAAGGGAATC AAACGGTCCT GCACCCTCCT 
CCTTCTAACA CCAAACAGGG AGAAAGGAAA CCTCCCAAGG TATTTGCATT TGATTATTGC 
TTTTGGTCCA TGGATGAATC CAACACTACA AAGTATGCTG GTCAAGAAGT GGTTTTCAAG 
TGCCTTGGGG AAGGAATTCT TGAAAAAGCC TTTCAGGGGT ATAATGCTTG CATTTTTGCA 
TATGGACAGA CAGGCTCAGG AAAATCCTTC TCCATGATGG GCAATGCTGA GCAGCGAGGC 
CTCATCCCAA GGCTCTGCTG TGCTTTATTT CAAAGGATCT CTCTGGAGCA GAATGAGTCA 
CAGACATTTA AAGTGGAAGT ATCCTATATG GAAATTTATA ATGAGAAAGT CCGGGACCTG 
TTAGACCCCA AAGGGAGTAG ACAGTCTCTT AAAGTTCGAG AGCATAAAGT TTTGGGACCA 
TATGTAGATG GTTTATCCCA ACTGGCTGTC ACTAGTTTTG AGGACATTGA GTCATTGATG 
TCTGAGGGAA ACAAATCTCG GACGGTAGCT GCGACCAACA TGAATGAAGA AAGCAGCCGC 
TCCCATGCAG TGTTCAACAT CGTCATCACG CAGACGCTGT ACGACCTGCA GTCTGGGAAC 
TCAGGGGAGA AGGTGAGCAA GGTGAGCTTG GTGGACCTGG CGGGCAGCGA GAGAGTGTCT 
AAAACTGGAG CTGCGGGAGA GCGCCTGAAA GAAGGCAGCA ACATTAACAA ATCACTTACA 
ACCTTGGGGT TGGTTATCTC ATCGCTGGCT GACCAGGCAG CTGGCAAGGG TAAAAACAAA 
TTTGTGCCTT ATCGCGACTC AGTCCTCACT TGGCTGCTTA AGGACAATCT GGGGGGCAAC 
AGCCAGACCT CCATGATCGC CACCATTAGC CCAGCAGCAG ACAACTACGA AGAGACCCTC 
TCCACGCTGA GATACGCAGA TCGGGCCAAA AGGATCGTTA ACCATGCCGT CGTGAATGAG 
GATCCCAACG CGAAGGTCAT CCGGGAACTG CGGGAAGAGG TGGAGAAACT GAGGGAGCAG 
CTCTCACAGG CTGAGGCTAT GAAGGCCCCT GAACTGAAAG AGAAGCTGGA AGAGTCTGAA 
AAGCTGATAA AAGAGCTAAC AGTGACTTGG GAAGAGAAGC TCAGGAAAAC AGAAGAAATT 
GCACAGGAGA GGCAGCGGCA ACTTGAAAGC ATGGGGATCT CCCTGGAGAC TTCTGGTATC 
AAAGTAGGAG ATGACAAGTG CTACTTGGTC AACCTGAATG CAGACCCTGC TCTCAACGAA 
CTTCTGGTTT ATTATTTAAA GGACCACACC AGGGTAGGTG CCGACACCTC TCAGGACATC 
CAGCTCTTTG GGATAGGAAT TCAGCCTGAG CACTGTGAGA TCGACATTGG CTCTGATGGA 
GAGGTCAGCC TCACTCCAAA AGAAAATGCA CGGTCCTGTG TCAATGGCAC CCTCGTGTGC 
AGCACCACCC AGCTGTGGCA TGGGGACCGA ATCCTGTGGG GAAACAACCA CTTCTTCAGG 
ATTAATTTGC CCAAGAGGAA ACGGCGAGAT TGGTTGAAAG ACTTTGAGAA AGAGACAGGC 
CCGCCGGAGC ACGACCTGGA CGCGGCCAGT GAAGCGTCCT CAGAACCAGA CTATAACTAC 
GAATTTGCGC AGATGGAGGT TATCATGAAA ACGCTGAATA GCAATGGTGG CTTCCCGATC 
ATAAGCCTGT CTGCCTGGTG GTCATCCCCT TGGTGGTCCT ATTTGAAGAT TCTCTTCCCT 
CTCATGTGGT TTCAGACGAA GCTCCTCCAC AAACACTGGG ATAAAGACCC AGTTCAGAAC 
GTGGTGCAGG TCTTGGAGAA ACAGTACCTG GAAGAAAAGC GAAGCGCCCT CGAGGAGCAG 
CGACTCATGT ACGAGCGGGA GCTGGAGCAG CTCCGCCAGC AGCTGTCCCC GGAGAGGCAG 
CCCCAGATCA GCGGCCCCGA CCGCCTGGCC TACAGCAGCC AGACGGCGCA GCAGAAGGTG 
ACCCAGTGGG CCGAGGAGAG GGACGAACTC TTTCGCCAAA GTCTGGCAAA ACTTCGGGAG 
CAGCTGGTGA AAGCCAACAC CCTGGTGAGA GAGGCAAACT TCTTAGCCGA GGAGATGAGC 
AAGCTCACCG ACTACCAGGT GACTCTGCAG ATCCCTGCTG CGAACCTCAG TGCCAACAGG 
AAGAGAGGAG CAATAGTGAG TGAGCCAGCT ATTCAAGTGA GGAGGAAAGG AAAGAGCACC 
CAAGTGTGGA CCATTGAGAA GCTGGAGAAT AAATTAATTG ATATGAGAGA CCTTTATCAA 
GAATGGAAAG AAAAGATTCC TGAGGCAAAG CGACTCTGCG GGAAACGAGG TGACCCTTTC 
TATGAAGCCC AAGAGAATCA CAACCTGATT GGCGTGGCGA ATGTGTTCCT GGAGTGTCTC 
TTCTGTGATG TGAAGCTTCA GTATGCAGTT CCTATCATCA GCCAGCAGGG GGAGGTTGCA 
GGGCGTCTCC ACGTGGAAGT GATGCGAGTG ACAGGCGCTG TCCCGGAGCG CATGGCGGAG 
GACGACTCCT CGGAGAACTC CAGTGAAAGC GGAAGTCTTG AAGTCGTAGA CAGCACCGGG 
GAAATCATTC ACCGAGTCAA GAAGCTGACA TGCCGGGTAA AAATTAAAGA AGCAACTGGG 
CTGCCCTTAA ACCTCTCAAA TTTCGTCTTC TGTCAATACA CGTTCTGGGA CCAGTGTGAG 
CCCACGGTGG CCGCCCCTGT CGTGGATCCT GAGGTGCCTT CCCCACAGTC CAAGGACGCC 
CAGTACACCG TGACCTTCTC CCACTGTCAG GATTACGTGG TCAACGTAAC GGAAGAATTC 
CTGGAGTTCA TTTCAGATGG AGCACTGGCC ATCGAAGTGT GGGGCCACCG GTGTACCGGG 
AACGGCAGCT CCATCTGGGA AGTCGATTCC CTTCATGCTA AGACGAGAAC GCTGCATGAC 
AGGTGGAATG AAGTTACTCG CAGAATAGAG ATGTGGATCT CCATCCTGGA GCTGAACGAG 
CTGGGGGAGT ACGCCGCCGT GGAGCTTCAC CAGGCCAAGG ACGTCAACAC AGGCGGCGTC 
CTTCAGCTCA GACAGGGTCA TTCCCGGAGA GTCCAAGTCA CAGTGAAGCC TGTCCAGCAT 
TCCGGGACAC TGCCACTCAT GGTTGAAGCC ATCTTGTCCA TCTCCATCGG CTGCATCACT 
GCCAGGTCAA CCAAGCTCCA GAGAGGGCTG GACAGTTACC AGAGAGATGA TGAGGATGGT 
GATGATATGG ATAGTTATCA GGAAGAAGAC TTAAACTGCG TGAGAGAGAG GTGGTCGGAT 
GCCCTCATTA AACGGCGAGA ATACCTAGAC GAACAGATTA AAAAAGTCAG CAACAAAAAA 
GAGAAGACGG AGGATGACGT GGAGCGGGAA GCCCGGCTGG TGGAGCAGTG GGTGGGGCTG 
ACGGAGGAGA GGAACGCTGT GCTGGTGCCT GCGCCGGGCA GCGGGATCCC CGGGGCGCCT 
GCCGACTGGA TCCCACCACC TGGAATGGAA ACCCACATAC CGGTTCTCTT CCTTGATTTG 
AACGCGGATG ACCTCAGTGC CAATGAGCAG CTCGTGGGCC CCCACGCATC AGGCGTGAAC 
TCCATCCTCC CCAAGGAGCA CGGCAGCCAG TTTTTCTACC TGCCCATCAT AAAGCACAGT 
GACGAGGAGG TTTCAGCCAC AGCCTCCTGG GACTCCTCGG TGCATGATTC CGTTCACTTG 
AACAGGGTCA CCCCGCAGAA TGAGAGGATC TACCTGATAG TGAAGACCAC AGTCCAGCTC 
AGCCACCCGG CCGCTATGGA GCTGGTCTTA CGAAAACGGA TTGCAGCCAA CATTTACAAC 
AAACAGAGTT TCACCCAGAG TTTGAAGAGG AGAATATCAC TGAAGAATAT ATTTTATTCC 
TGTGGTGTAA CCTATGAAAT AGTATCCAAC ATACCAAAGG CAACAGAGGA GATTGAGGAC 
CGGGAGACGC TGGCTCTCAT GGCTGCAAGG AGTGAGAATG AAGGCACGTC GGATGGGGAG 
ACGTACATCG AGAAGTACAC GCGGGGCGTG CTGCAGGTGG AGAACATTCT GAGCCTGGAA 
CGACTCCGGC AGGCGGTGAC GGTCAAAGAA GCACTTTCCA CCAAAGCTCG GCACCTACGG 
CGAAGCCTCA GCACGCCCAA CGTTCACAAT GTCTCTTCCA GTCGACCAGA CCTTTCTGGC 
TTTGACGATG ATGATAAGGG TTGGCCAGAG AACCAGTTAG ACATGTCTGA CTACAGCCCG 
AGTTACCAGG ATGTATCGTG TTATGGAACT TTGCCTAGGG ATTCACCTCG AAGGAGTAAA 
GAAGGACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816858.2
CDS	89..4540
Translation	MSDTKVKVAVRVRPLNRRELELNTKCVVEMEGNQTVLHPPPSNTKQGERKPPKVFAFDYCFWSMDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGL

Target ORF information:

RefSeq Version	XM_010818556.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 13A (KIF13A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818556.3

ORF Insert Sequence:

ATGTCGGATA CCAAGGTAAA AGTTGCCGTC CGGGTCCGGC CCCTGAACCG ACGAGAACTG 
GAACTCAACA CCAAGTGCGT GGTGGAGATG GAAGGGAATC AAACGGTCCT GCACCCTCCT 
CCTTCTAACA CCAAACAGGG AGAAAGGAAA CCTCCCAAGG TATTTGCATT TGATTATTGC 
TTTTGGTCCA TGGATGAATC CAACACTACA AAGTATGCTG GTCAAGAAGT GGTTTTCAAG 
TGCCTTGGGG AAGGAATTCT TGAAAAAGCC TTTCAGGGGT ATAATGCTTG CATTTTTGCA 
TATGGACAGA CAGGCTCAGG AAAATCCTTC TCCATGATGG GCAATGCTGA GCAGCGAGGC 
CTCATCCCAA GGCTCTGCTG TGCTTTATTT CAAAGGATCT CTCTGGAGCA GAATGAGTCA 
CAGACATTTA AAGTGGAAGT ATCCTATATG GAAATTTATA ATGAGAAAGT CCGGGACCTG 
TTAGACCCCA AAGGGAGTAG ACAGTCTCTT AAAGTTCGAG AGCATAAAGT TTTGGGACCA 
TATGTAGATG GTTTATCCCA ACTGGCTGTC ACTAGTTTTG AGGACATTGA GTCATTGATG 
TCTGAGGGAA ACAAATCTCG GACGGTAGCT GCGACCAACA TGAATGAAGA AAGCAGCCGC 
TCCCATGCAG TGTTCAACAT CGTCATCACG CAGACGCTGT ACGACCTGCA GTCTGGGAAC 
TCAGGGGAGA AGGTGAGCAA GGTGAGCTTG GTGGACCTGG CGGGCAGCGA GAGAGTGTCT 
AAAACTGGAG CTGCGGGAGA GCGCCTGAAA GAAGGCAGCA ACATTAACAA ATCACTTACA 
ACCTTGGGGT TGGTTATCTC ATCGCTGGCT GACCAGGCAG CTGGCAAGGG TAAAAACAAA 
TTTGTGCCTT ATCGCGACTC AGTCCTCACT TGGCTGCTTA AGGACAATCT GGGGGGCAAC 
AGCCAGACCT CCATGATCGC CACCATTAGC CCAGCAGCAG ACAACTACGA AGAGACCCTC 
TCCACGCTGA GATACGCAGA TCGGGCCAAA AGGATCGTTA ACCATGCCGT CGTGAATGAG 
GATCCCAACG CGAAGGTCAT CCGGGAACTG CGGGAAGAGG TGGAGAAACT GAGGGAGCAG 
CTCTCACAGG CTGAGGCTAT GAAGGCCCCT GAACTGAAAG AGAAGCTGGA AGAGTCTGAA 
AAGCTGATAA AAGAGCTAAC AGTGACTTGG GAAGAGAAGC TCAGGAAAAC AGAAGAAATT 
GCACAGGAGA GGCAGCGGCA ACTTGAAAGC ATGGGGATCT CCCTGGAGAC TTCTGGTATC 
AAAGTAGGAG ATGACAAGTG CTACTTGGTC AACCTGAATG CAGACCCTGC TCTCAACGAA 
CTTCTGGTTT ATTATTTAAA GGACCACACC AGGGTAGGTG CCGACACCTC TCAGGACATC 
CAGCTCTTTG GGATAGGAAT TCAGCCTGAG CACTGTGAGA TCGACATTGG CTCTGATGGA 
GAGGTCAGCC TCACTCCAAA AGAAAATGCA CGGTCCTGTG TCAATGGCAC CCTCGTGTGC 
AGCACCACCC AGCTGTGGCA TGGGGACCGA ATCCTGTGGG GAAACAACCA CTTCTTCAGG 
ATTAATTTGC CCAAGAGGAA ACGGCGAGAT TGGTTGAAAG ACTTTGAGAA AGAGACAGGC 
CCGCCGGAGC ACGACCTGGA CGCGGCCAGT GAAGCGTCCT CAGAACCAGA CTATAACTAC 
GAATTTGCGC AGATGGAGGT TATCATGAAA ACGCTGAATA GCAATGGTGG CTTCCCGATC 
ATAAGCCTGT CTGCCTGGTG GTCATCCCCT TGGTGGTCCT ATTTGAAGAT TCTCTTCCCT 
CTCATGTGGT TTCAGACGAA GCTCCTCCAC AAACACTGGG ATAAAGACCC AGTTCAGAAC 
GTGGTGCAGG TCTTGGAGAA ACAGTACCTG GAAGAAAAGC GAAGCGCCCT CGAGGAGCAG 
CGACTCATGT ACGAGCGGGA GCTGGAGCAG CTCCGCCAGC AGCTGTCCCC GGAGAGGCAG 
CCCCAGATCA GCGGCCCCGA CCGCCTGGCC TACAGCAGCC AGACGGCGCA GCAGAAGGTG 
ACCCAGTGGG CCGAGGAGAG GGACGAACTC TTTCGCCAAA GTCTGGCAAA ACTTCGGGAG 
CAGCTGGTGA AAGCCAACAC CCTGGTGAGA GAGGCAAACT TCTTAGCCGA GGAGATGAGC 
AAGCTCACCG ACTACCAGGT GACTCTGCAG ATCCCTGCTG CGAACCTCAG TGCCAACAGG 
AAGAGAGGAG CAATAGTGAG TGAGCCAGCT ATTCAAGTGA GGAGGAAAGG AAAGAGCACC 
CAAGTGTGGA CCATTGAGAA GCTGGAGAAT AAATTAATTG ATATGAGAGA CCTTTATCAA 
GAATGGAAAG AAAAGATTCC TGAGGCAAAG CGACTCTGCG GGAAACGAGG TGACCCTTTC 
TATGAAGCCC AAGAGAATCA CAACCTGATT GGCGTGGCGA ATGTGTTCCT GGAGTGTCTC 
TTCTGTGATG TGAAGCTTCA GTATGCAGTT CCTATCATCA GCCAGCAGGG GGAGGTTGCA 
GGGCGTCTCC ACGTGGAAGT GATGCGAGTG ACAGGCGCTG TCCCGGAGCG CATGGCGGAG 
GACGACTCCT CGGAGAACTC CAGTGAAAGC GGAAGTCTTG AAGTCGTAGA CAGCACCGGG 
GAAATCATTC ACCGAGTCAA GAAGCTGACA TGCCGGGTAA AAATTAAAGA AGCAACTGGG 
CTGCCCTTAA ACCTCTCAAA TTTCGTCTTC TGTCAATACA CGTTCTGGGA CCAGTGTGAG 
CCCACGGTGG CCGCCCCTGT CGTGGATCCT GAGGTGCCTT CCCCACAGTC CAAGGACGCC 
CAGTACACCG TGACCTTCTC CCACTGTCAG GATTACGTGG TCAACGTAAC GGAAGAATTC 
CTGGAGTTCA TTTCAGATGG AGCACTGGCC ATCGAAGTGT GGGGCCACCG GTGTACCGGG 
AACGGCAGCT CCATCTGGGA AGTCGATTCC CTTCATGCTA AGACGAGAAC GCTGCATGAC 
AGGTGGAATG AAGTTACTCG CAGAATAGAG ATGTGGATCT CCATCCTGGA GCTGAACGAG 
CTGGGGGAGT ACGCCGCCGT GGAGCTTCAC CAGGCCAAGG ACGTCAACAC AGGCGGCGTC 
CTTCAGCTCA GACAGGGTCA TTCCCGGAGA GTCCAAGTCA CAGTGAAGCC TGTCCAGCAT 
TCCGGGACAC TGCCACTCAT GGTTGAAGCC ATCTTGTCCA TCTCCATCGG CTGCATCACT 
GCCAGGTCAA CCAAGCTCCA GAGAGGGCTG GACAGTTACC AGAGAGATGA TGAGGATGGT 
GATGATATGG ATAGTTATCA GGAAGAAGAC TTAAACTGCG TGAGAGAGAG GTGGTCGGAT 
GCCCTCATTA AACGGCGAGA ATACCTAGAC GAACAGATTA AAAAAGTCAG CAACAAAAAA 
GAGAAGACGG AGGATGACGT GGAGCGGGAA GCCCGGCTGG TGGAGCAGTG GGTGGGGCTG 
ACGGAGGAGA GGAACGCTGT GCTGGTGCCT GCGCCGGGCA GCGGGATCCC CGGGGCGCCT 
GCCGACTGGA TCCCACCACC TGGAATGGAA ACCCACATAC CGGTTCTCTT CCTTGATTTG 
AACGCGGATG ACCTCAGTGC CAATGAGCAG CTCGTGGGCC CCCACGCATC AGGCGTGAAC 
TCCATCCTCC CCAAGGAGCA CGGCAGCCAG TTTTTCTACC TGCCCATCAT AAAGCACAGT 
GACGAGGAGG TTTCAGCCAC AGCCTCCTGG GACTCCTCGG TGCATGATTC CGTTCACTTG 
AACAGGGTCA CCCCGCAGAA TGAGAGGATC TACCTGATAG TGAAGACCAC AGTCCAGCTC 
AGCCACCCGG CCGCTATGGA GCTGGTCTTA CGAAAACGGA TTGCAGCCAA CATTTACAAC 
AAACAGAGTT TCACCCAGAG TTTGAAGAGG AGAATATCAC TGAAGAATAT ATTTTATTCC 
TGTGGTGTAA CCTATGAAAT AGTATCCAAC ATACCAAAGG CAACAGAGGA GATTGAGGAC 
CGGGAGACGC TGGCTCTCAT GGCTGCAAGG AGTGAGAATG AAGGCACGTC GGATGGGGAG 
ACGTACATCG AGAAGTACAC GCGGGGCGTG CTGCAGGTGG AGAACATTCT GAGCCTGGAA 
CGACTCCGGC AGGCGGTGAC GGTCAAAGAA GCACTTTCCA CCAAAGCTCG GCACCTACGG 
CGAAGCCTCA GCACGCCCAA CGTTCACAAT GTCTCTTCCA GTCGACCAGA CCTTTCTGGC 
TTTGACGATG ATGATAAGGG TTGGCCAGAG AACCAGTTAG ACATGTCTGA CTACAGCCCG 
AGTTACCAGG ATGTATCGTG TTATGGAACT TTGCCTAGGG ATTCACCTCG AAGGAGTAAA 
GAAGGACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_010818549.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816851.2 Bos taurus kinesin family member 13A (KIF13A), transcript variant X1, mRNA.

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CloneID	OBa229709
Clone ID Related Accession (Same CDS sequence)	XM_010818549.3
Accession Version	XM_010818549.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818549.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGATA CCAAGGTAAA AGTTGCCGTC CGGGTCCGGC CCCTGAACCG ACGAGAACTG 
GAACTCAACA CCAAGTGCGT GGTGGAGATG GAAGGGAATC AAACGGTCCT GCACCCTCCT 
CCTTCTAACA CCAAACAGGG AGAAAGGAAA CCTCCCAAGG TATTTGCATT TGATTATTGC 
TTTTGGTCCA TGGATGAATC CAACACTACA AAGTATGCTG GTCAAGAAGT GGTTTTCAAG 
TGCCTTGGGG AAGGAATTCT TGAAAAAGCC TTTCAGGGGT ATAATGCTTG CATTTTTGCA 
TATGGACAGA CAGGCTCAGG AAAATCCTTC TCCATGATGG GCAATGCTGA GCAGCGAGGC 
CTCATCCCAA GGCTCTGCTG TGCTTTATTT CAAAGGATCT CTCTGGAGCA GAATGAGTCA 
CAGACATTTA AAGTGGAAGT ATCCTATATG GAAATTTATA ATGAGAAAGT CCGGGACCTG 
TTAGACCCCA AAGGGAGTAG ACAGTCTCTT AAAGTTCGAG AGCATAAAGT TTTGGGACCA 
TATGTAGATG GTTTATCCCA ACTGGCTGTC ACTAGTTTTG AGGACATTGA GTCATTGATG 
TCTGAGGGAA ACAAATCTCG GACGGTAGCT GCGACCAACA TGAATGAAGA AAGCAGCCGC 
TCCCATGCAG TGTTCAACAT CGTCATCACG CAGACGCTGT ACGACCTGCA GTCTGGGAAC 
TCAGGGGAGA AGGTGAGCAA GGTGAGCTTG GTGGACCTGG CGGGCAGCGA GAGAGTGTCT 
AAAACTGGAG CTGCGGGAGA GCGCCTGAAA GAAGGCAGCA ACATTAACAA ATCACTTACA 
ACCTTGGGGT TGGTTATCTC ATCGCTGGCT GACCAGGCAG CTGGCAAGGG TAAAAACAAA 
TTTGTGCCTT ATCGCGACTC AGTCCTCACT TGGCTGCTTA AGGACAATCT GGGGGGCAAC 
AGCCAGACCT CCATGATCGC CACCATTAGC CCAGCAGCAG ACAACTACGA AGAGACCCTC 
TCCACGCTGA GATACGCAGA TCGGGCCAAA AGGATCGTTA ACCATGCCGT CGTGAATGAG 
GATCCCAACG CGAAGGTCAT CCGGGAACTG CGGGAAGAGG TGGAGAAACT GAGGGAGCAG 
CTCTCACAGG CTGAGGCTAT GAAGGCCCCT GAACTGAAAG AGAAGCTGGA AGAGTCTGAA 
AAGCTGATAA AAGAGCTAAC AGTGACTTGG GAAGAGAAGC TCAGGAAAAC AGAAGAAATT 
GCACAGGAGA GGCAGCGGCA ACTTGAAAGC ATGGGGATCT CCCTGGAGAC TTCTGGTATC 
AAAGTAGGAG ATGACAAGTG CTACTTGGTC AACCTGAATG CAGACCCTGC TCTCAACGAA 
CTTCTGGTTT ATTATTTAAA GGACCACACC AGGGTAGGTG CCGACACCTC TCAGGACATC 
CAGCTCTTTG GGATAGGAAT TCAGCCTGAG CACTGTGAGA TCGACATTGG CTCTGATGGA 
GAGGTCAGCC TCACTCCAAA AGAAAATGCA CGGTCCTGTG TCAATGGCAC CCTCGTGTGC 
AGCACCACCC AGCTGTGGCA TGGGGACCGA ATCCTGTGGG GAAACAACCA CTTCTTCAGG 
ATTAATTTGC CCAAGAGGAA ACGGCGAGAT TGGTTGAAAG ACTTTGAGAA AGAGACAGGC 
CCGCCGGAGC ACGACCTGGA CGCGGCCAGT GAAGCGTCCT CAGAACCAGA CTATAACTAC 
GAATTTGCGC AGATGGAGGT TATCATGAAA ACGCTGAATA GCAATGGTGG CTTCCCGATC 
ATAAGCCTGT CTGCCTGGTG GTCATCCCCT TGGTGGTCCT ATTTGAAGAT TCTCTTCCCT 
CTCATGTGGT TTCAGACGAA GCTCCTCCAC AAACACTGGG ATAAAGACCC AGTTCAGAAC 
GTGGTGCAGG TCTTGGAGAA ACAGTACCTG GAAGAAAAGC GAAGCGCCCT CGAGGAGCAG 
CGACTCATGT ACGAGCGGGA GCTGGAGCAG CTCCGCCAGC AGCTGTCCCC GGAGAGGCAG 
CCCCAGATCA GCGGCCCCGA CCGCCTGGCC TACAGCAGCC AGACGGCGCA GCAGAAGGTG 
ACCCAGTGGG CCGAGGAGAG GGACGAACTC TTTCGCCAAA GTCTGGCAAA ACTTCGGGAG 
CAGCTGGTGA AAGCCAACAC CCTGGTGAGA GAGGCAAACT TCTTAGCCGA GGAGATGAGC 
AAGCTCACCG ACTACCAGGT GACTCTGCAG ATCCCTGCTG CGAACCTCAG TGCCAACAGG 
AAGAGAGGAG CAATAGTGAG TGAGCCAGCT ATTCAAGTGA GGAGGAAAGG AAAGAGCACC 
CAAGTGTGGA CCATTGAGAA GCTGGAGAAT AAATTAATTG ATATGAGAGA CCTTTATCAA 
GAATGGAAAG AAAAGATTCC TGAGGCAAAG CGACTCTGCG GGAAACGAGG TGACCCTTTC 
TATGAAGCCC AAGAGAATCA CAACCTGATT GGCGTGGCGA ATGTGTTCCT GGAGTGTCTC 
TTCTGTGATG TGAAGCTTCA GTATGCAGTT CCTATCATCA GCCAGCAGGG GGAGGTTGCA 
GGGCGTCTCC ACGTGGAAGT GATGCGAGTG ACAGGCGCTG TCCCGGAGCG CATGGCGGAG 
GACGACTCCT CGGAGAACTC CAGTGAAAGC GGAAGTCTTG AAGTCGTAGA CAGCACCGGG 
GAAATCATTC ACCGAGTCAA GAAGCTGACA TGCCGGGTAA AAATTAAAGA AGCAACTGGG 
CTGCCCTTAA ACCTCTCAAA TTTCGTCTTC TGTCAATACA CGTTCTGGGA CCAGTGTGAG 
CCCACGGTGG CCGCCCCTGT CGTGGATCCT GAGGTGCCTT CCCCACAGTC CAAGGACGCC 
CAGTACACCG TGACCTTCTC CCACTGTCAG GATTACGTGG TCAACGTAAC GGAAGAATTC 
CTGGAGTTCA TTTCAGATGG AGCACTGGCC ATCGAAGTGT GGGGCCACCG GTGTACCGGG 
AACGGCAGCT CCATCTGGGA AGTCGATTCC CTTCATGCTA AGACGAGAAC GCTGCATGAC 
AGGTGGAATG AAGTTACTCG CAGAATAGAG ATGTGGATCT CCATCCTGGA GCTGAACGAG 
CTGGGGGAGT ACGCCGCCGT GGAGCTTCAC CAGGCCAAGG ACGTCAACAC AGGCGGCGTC 
CTTCAGCTCA GACAGGGTCA TTCCCGGAGA GTCCAAGTCA CAGTGAAGCC TGTCCAGCAT 
TCCGGGACAC TGCCACTCAT GGTTGAAGCC ATCTTGTCCA TCTCCATCGG CTGCATCACT 
GCCAGGTCAA CCAAGCTCCA GAGAGGGCTG GACAGTTACC AGAGAGATGA TGAGGATGGT 
GATGATATGG ATAGTTATCA GGAAGAAGAC TTAAACTGCG TGAGAGAGAG GTGGTCGGAT 
GCCCTCATTA AACGGCGAGA ATACCTAGAC GAACAGATTA AAAAAGTCAG CAACAAAAAA 
GAGAAGACGG AGGATGACGT GGAGCGGGAA GCCCGGCTGG TGGAGCAGTG GGTGGGGCTG 
ACGGAGGAGA GGAACGCTGT GCTGGTGCCT GCGCCGGGCA GCGGGATCCC CGGGGCGCCT 
GCCGACTGGA TCCCACCACC TGGAATGGAA ACCCACATAC CGGTTCTCTT CCTTGATTTG 
AACGCGGATG ACCTCAGTGC CAATGAGCAG CTCGTGGGCC CCCACGCATC AGGCGTGAAC 
TCCATCCTCC CCAAGGAGCA CGGCAGCCAG TTTTTCTACC TGCCCATCAT AAAGCACAGT 
GACGAGGAGG TTTCAGCCAC AGCCTCCTGG GACTCCTCGG TGCATGATTC CGTTCACTTG 
AACAGGGTCA CCCCGCAGAA TGAGAGGATC TACCTGATAG TGAAGACCAC AGTCCAGCTC 
AGCCACCCGG CCGCTATGGA GCTGGTCTTA CGAAAACGGA TTGCAGCCAA CATTTACAAC 
AAACAGAGTT TCACCCAGAG TTTGAAGAGG AGAATATCAC TGAAGAATAT ATTTTATTCC 
TGTGGTGTAA CCTATGAAAT AGTATCCAAC ATACCAAAGG CAACAGAGGA GATTGAGGAC 
CGGGAGACGC TGGCTCTCAT GGCTGCAAGG AGTGAGAATG AAGGCACGTC GGATGGGGAG 
ACGTACATCG AGAAGTACAC GCGGGGCGTG CTGCAGGTGG AGAACATTCT GAGCCTGGAA 
CGACTCCGGC AGGCGGTGAC GGTCAAAGAA GCACTTTCCA CCAAAGCTCG GCACCTACGG 
CGAAGCCTCA GCACGCCCAA CGTTCACAAT GTCTCTTCCA GTCGACCAGA CCTTTCTGGC 
TTTGACGATG ATGATAAGGG TTGGCCAGAG AACCAGTTAG ACATGTCTGA CTACAGCCCG 
AGTTACCAGG ATGTATCGTG TTATGGAACT TTGCCTAGGG ATTCACCTCG AAGGAGTAAA 
GAAGGTTGTA CATCAGAGAA TCCTCATGCC TTAACAGTCA GCCCTTTCAA AGCATTCTCT 
CCTCAGCCAC CAAAGTTTTT CAAGCCCCTA ATGCCTGTAA AAGAGGAGCA TAAGAAAAGG 
ATAGCGCTTG AAGCAAGGCC TCTTCTAAGC CAGGAGAGCA TGTCTCCATC TCAGGCACAT 
AATCCTGGCT GCATTGTGCC CTCGGGAAGC AATGGCAGCA GCATGCCAGT AGAACTCAAT 
AGCAAACGTG AGAAGAAGAT TGACTCTGAG GAGGAGGAAA ATGATCTAGA AGGTCTCAGC 
CGGAAGCTGA TAAGTTCACA GCCTTATGTA CCTGTGGAGT TCGCTGACTT CAGCGTTTAC 
AATGCCAGCT TGGAGAACAG AGAGTGGTTT TCTTCTAAAG GCGATCTGAC GAACTCAAAG 
GTCCTAGAGA AGGAGGTGTC CCGCAGCCCC ACCACCAGCA GCATCACCAG CGGCTACTTT 
TCCCACAGTG CCTCCAACGC CACCCTGTCC GACATGGTGG TCCCTTCCAG TGACAGCTCA 
GATCAGTTGG CCCTTCCAAC GAAGGACACA GATTCCAGCG AGCATCCCGG ACCGTCCCTT 
GGGCAGGATT TCAGACCATC TTCAAACAAA GAGTTGACAG AACTAGAAAG AGGCTTGGGA 
AAGGATAAGA TGACCGTGGT GCCACTCAAG GAAAACAGTG CCTTAGCCAA AGGGAGCCCA 
TTGTCCCCCA GTATCCCGGA GAAAAACTCC AGAACCCTCT GTAGGACCAG CTCGTGTTCA 
GAGCAGGATG CCTGCTCCAG CAAAAACGGC CAGCCGGCCA GGGAATTCTG CCCCAGGGAG 
GTGACCGTGG AACACACCAC AAACATCCTC GAGGATCACT CTTTCACAGA GTTTATGGGT 
GTGTCAGATG GGAAAGATTT TGATGGTTTG ACGGATTCTT CTGCTGGAGA ACTTTCAAGT 
AGGAGGAACC TACCAAATAC AGCGGACAGT AGGAGTTTCT CACATGGGCC ACAGGACCCT 
GGCCAGCTGT GTTCCTCGGC AGAGAATGAC CAGGGGAGCG GCACTGCTCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816851.2
CDS	89..5602
Translation	MSDTKVKVAVRVRPLNRRELELNTKCVVEMEGNQTVLHPPPSNTKQGERKPPKVFAFDYCFWSMDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQESMSPSQAHNPGCIVPSGSNGSSMPVELNSKREKKIDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_010818549.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 13A (KIF13A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818549.3

ORF Insert Sequence:

ATGTCGGATA CCAAGGTAAA AGTTGCCGTC CGGGTCCGGC CCCTGAACCG ACGAGAACTG 
GAACTCAACA CCAAGTGCGT GGTGGAGATG GAAGGGAATC AAACGGTCCT GCACCCTCCT 
CCTTCTAACA CCAAACAGGG AGAAAGGAAA CCTCCCAAGG TATTTGCATT TGATTATTGC 
TTTTGGTCCA TGGATGAATC CAACACTACA AAGTATGCTG GTCAAGAAGT GGTTTTCAAG 
TGCCTTGGGG AAGGAATTCT TGAAAAAGCC TTTCAGGGGT ATAATGCTTG CATTTTTGCA 
TATGGACAGA CAGGCTCAGG AAAATCCTTC TCCATGATGG GCAATGCTGA GCAGCGAGGC 
CTCATCCCAA GGCTCTGCTG TGCTTTATTT CAAAGGATCT CTCTGGAGCA GAATGAGTCA 
CAGACATTTA AAGTGGAAGT ATCCTATATG GAAATTTATA ATGAGAAAGT CCGGGACCTG 
TTAGACCCCA AAGGGAGTAG ACAGTCTCTT AAAGTTCGAG AGCATAAAGT TTTGGGACCA 
TATGTAGATG GTTTATCCCA ACTGGCTGTC ACTAGTTTTG AGGACATTGA GTCATTGATG 
TCTGAGGGAA ACAAATCTCG GACGGTAGCT GCGACCAACA TGAATGAAGA AAGCAGCCGC 
TCCCATGCAG TGTTCAACAT CGTCATCACG CAGACGCTGT ACGACCTGCA GTCTGGGAAC 
TCAGGGGAGA AGGTGAGCAA GGTGAGCTTG GTGGACCTGG CGGGCAGCGA GAGAGTGTCT 
AAAACTGGAG CTGCGGGAGA GCGCCTGAAA GAAGGCAGCA ACATTAACAA ATCACTTACA 
ACCTTGGGGT TGGTTATCTC ATCGCTGGCT GACCAGGCAG CTGGCAAGGG TAAAAACAAA 
TTTGTGCCTT ATCGCGACTC AGTCCTCACT TGGCTGCTTA AGGACAATCT GGGGGGCAAC 
AGCCAGACCT CCATGATCGC CACCATTAGC CCAGCAGCAG ACAACTACGA AGAGACCCTC 
TCCACGCTGA GATACGCAGA TCGGGCCAAA AGGATCGTTA ACCATGCCGT CGTGAATGAG 
GATCCCAACG CGAAGGTCAT CCGGGAACTG CGGGAAGAGG TGGAGAAACT GAGGGAGCAG 
CTCTCACAGG CTGAGGCTAT GAAGGCCCCT GAACTGAAAG AGAAGCTGGA AGAGTCTGAA 
AAGCTGATAA AAGAGCTAAC AGTGACTTGG GAAGAGAAGC TCAGGAAAAC AGAAGAAATT 
GCACAGGAGA GGCAGCGGCA ACTTGAAAGC ATGGGGATCT CCCTGGAGAC TTCTGGTATC 
AAAGTAGGAG ATGACAAGTG CTACTTGGTC AACCTGAATG CAGACCCTGC TCTCAACGAA 
CTTCTGGTTT ATTATTTAAA GGACCACACC AGGGTAGGTG CCGACACCTC TCAGGACATC 
CAGCTCTTTG GGATAGGAAT TCAGCCTGAG CACTGTGAGA TCGACATTGG CTCTGATGGA 
GAGGTCAGCC TCACTCCAAA AGAAAATGCA CGGTCCTGTG TCAATGGCAC CCTCGTGTGC 
AGCACCACCC AGCTGTGGCA TGGGGACCGA ATCCTGTGGG GAAACAACCA CTTCTTCAGG 
ATTAATTTGC CCAAGAGGAA ACGGCGAGAT TGGTTGAAAG ACTTTGAGAA AGAGACAGGC 
CCGCCGGAGC ACGACCTGGA CGCGGCCAGT GAAGCGTCCT CAGAACCAGA CTATAACTAC 
GAATTTGCGC AGATGGAGGT TATCATGAAA ACGCTGAATA GCAATGGTGG CTTCCCGATC 
ATAAGCCTGT CTGCCTGGTG GTCATCCCCT TGGTGGTCCT ATTTGAAGAT TCTCTTCCCT 
CTCATGTGGT TTCAGACGAA GCTCCTCCAC AAACACTGGG ATAAAGACCC AGTTCAGAAC 
GTGGTGCAGG TCTTGGAGAA ACAGTACCTG GAAGAAAAGC GAAGCGCCCT CGAGGAGCAG 
CGACTCATGT ACGAGCGGGA GCTGGAGCAG CTCCGCCAGC AGCTGTCCCC GGAGAGGCAG 
CCCCAGATCA GCGGCCCCGA CCGCCTGGCC TACAGCAGCC AGACGGCGCA GCAGAAGGTG 
ACCCAGTGGG CCGAGGAGAG GGACGAACTC TTTCGCCAAA GTCTGGCAAA ACTTCGGGAG 
CAGCTGGTGA AAGCCAACAC CCTGGTGAGA GAGGCAAACT TCTTAGCCGA GGAGATGAGC 
AAGCTCACCG ACTACCAGGT GACTCTGCAG ATCCCTGCTG CGAACCTCAG TGCCAACAGG 
AAGAGAGGAG CAATAGTGAG TGAGCCAGCT ATTCAAGTGA GGAGGAAAGG AAAGAGCACC 
CAAGTGTGGA CCATTGAGAA GCTGGAGAAT AAATTAATTG ATATGAGAGA CCTTTATCAA 
GAATGGAAAG AAAAGATTCC TGAGGCAAAG CGACTCTGCG GGAAACGAGG TGACCCTTTC 
TATGAAGCCC AAGAGAATCA CAACCTGATT GGCGTGGCGA ATGTGTTCCT GGAGTGTCTC 
TTCTGTGATG TGAAGCTTCA GTATGCAGTT CCTATCATCA GCCAGCAGGG GGAGGTTGCA 
GGGCGTCTCC ACGTGGAAGT GATGCGAGTG ACAGGCGCTG TCCCGGAGCG CATGGCGGAG 
GACGACTCCT CGGAGAACTC CAGTGAAAGC GGAAGTCTTG AAGTCGTAGA CAGCACCGGG 
GAAATCATTC ACCGAGTCAA GAAGCTGACA TGCCGGGTAA AAATTAAAGA AGCAACTGGG 
CTGCCCTTAA ACCTCTCAAA TTTCGTCTTC TGTCAATACA CGTTCTGGGA CCAGTGTGAG 
CCCACGGTGG CCGCCCCTGT CGTGGATCCT GAGGTGCCTT CCCCACAGTC CAAGGACGCC 
CAGTACACCG TGACCTTCTC CCACTGTCAG GATTACGTGG TCAACGTAAC GGAAGAATTC 
CTGGAGTTCA TTTCAGATGG AGCACTGGCC ATCGAAGTGT GGGGCCACCG GTGTACCGGG 
AACGGCAGCT CCATCTGGGA AGTCGATTCC CTTCATGCTA AGACGAGAAC GCTGCATGAC 
AGGTGGAATG AAGTTACTCG CAGAATAGAG ATGTGGATCT CCATCCTGGA GCTGAACGAG 
CTGGGGGAGT ACGCCGCCGT GGAGCTTCAC CAGGCCAAGG ACGTCAACAC AGGCGGCGTC 
CTTCAGCTCA GACAGGGTCA TTCCCGGAGA GTCCAAGTCA CAGTGAAGCC TGTCCAGCAT 
TCCGGGACAC TGCCACTCAT GGTTGAAGCC ATCTTGTCCA TCTCCATCGG CTGCATCACT 
GCCAGGTCAA CCAAGCTCCA GAGAGGGCTG GACAGTTACC AGAGAGATGA TGAGGATGGT 
GATGATATGG ATAGTTATCA GGAAGAAGAC TTAAACTGCG TGAGAGAGAG GTGGTCGGAT 
GCCCTCATTA AACGGCGAGA ATACCTAGAC GAACAGATTA AAAAAGTCAG CAACAAAAAA 
GAGAAGACGG AGGATGACGT GGAGCGGGAA GCCCGGCTGG TGGAGCAGTG GGTGGGGCTG 
ACGGAGGAGA GGAACGCTGT GCTGGTGCCT GCGCCGGGCA GCGGGATCCC CGGGGCGCCT 
GCCGACTGGA TCCCACCACC TGGAATGGAA ACCCACATAC CGGTTCTCTT CCTTGATTTG 
AACGCGGATG ACCTCAGTGC CAATGAGCAG CTCGTGGGCC CCCACGCATC AGGCGTGAAC 
TCCATCCTCC CCAAGGAGCA CGGCAGCCAG TTTTTCTACC TGCCCATCAT AAAGCACAGT 
GACGAGGAGG TTTCAGCCAC AGCCTCCTGG GACTCCTCGG TGCATGATTC CGTTCACTTG 
AACAGGGTCA CCCCGCAGAA TGAGAGGATC TACCTGATAG TGAAGACCAC AGTCCAGCTC 
AGCCACCCGG CCGCTATGGA GCTGGTCTTA CGAAAACGGA TTGCAGCCAA CATTTACAAC 
AAACAGAGTT TCACCCAGAG TTTGAAGAGG AGAATATCAC TGAAGAATAT ATTTTATTCC 
TGTGGTGTAA CCTATGAAAT AGTATCCAAC ATACCAAAGG CAACAGAGGA GATTGAGGAC 
CGGGAGACGC TGGCTCTCAT GGCTGCAAGG AGTGAGAATG AAGGCACGTC GGATGGGGAG 
ACGTACATCG AGAAGTACAC GCGGGGCGTG CTGCAGGTGG AGAACATTCT GAGCCTGGAA 
CGACTCCGGC AGGCGGTGAC GGTCAAAGAA GCACTTTCCA CCAAAGCTCG GCACCTACGG 
CGAAGCCTCA GCACGCCCAA CGTTCACAAT GTCTCTTCCA GTCGACCAGA CCTTTCTGGC 
TTTGACGATG ATGATAAGGG TTGGCCAGAG AACCAGTTAG ACATGTCTGA CTACAGCCCG 
AGTTACCAGG ATGTATCGTG TTATGGAACT TTGCCTAGGG ATTCACCTCG AAGGAGTAAA 
GAAGGTTGTA CATCAGAGAA TCCTCATGCC TTAACAGTCA GCCCTTTCAA AGCATTCTCT 
CCTCAGCCAC CAAAGTTTTT CAAGCCCCTA ATGCCTGTAA AAGAGGAGCA TAAGAAAAGG 
ATAGCGCTTG AAGCAAGGCC TCTTCTAAGC CAGGAGAGCA TGTCTCCATC TCAGGCACAT 
AATCCTGGCT GCATTGTGCC CTCGGGAAGC AATGGCAGCA GCATGCCAGT AGAACTCAAT 
AGCAAACGTG AGAAGAAGAT TGACTCTGAG GAGGAGGAAA ATGATCTAGA AGGTCTCAGC 
CGGAAGCTGA TAAGTTCACA GCCTTATGTA CCTGTGGAGT TCGCTGACTT CAGCGTTTAC 
AATGCCAGCT TGGAGAACAG AGAGTGGTTT TCTTCTAAAG GCGATCTGAC GAACTCAAAG 
GTCCTAGAGA AGGAGGTGTC CCGCAGCCCC ACCACCAGCA GCATCACCAG CGGCTACTTT 
TCCCACAGTG CCTCCAACGC CACCCTGTCC GACATGGTGG TCCCTTCCAG TGACAGCTCA 
GATCAGTTGG CCCTTCCAAC GAAGGACACA GATTCCAGCG AGCATCCCGG ACCGTCCCTT 
GGGCAGGATT TCAGACCATC TTCAAACAAA GAGTTGACAG AACTAGAAAG AGGCTTGGGA 
AAGGATAAGA TGACCGTGGT GCCACTCAAG GAAAACAGTG CCTTAGCCAA AGGGAGCCCA 
TTGTCCCCCA GTATCCCGGA GAAAAACTCC AGAACCCTCT GTAGGACCAG CTCGTGTTCA 
GAGCAGGATG CCTGCTCCAG CAAAAACGGC CAGCCGGCCA GGGAATTCTG CCCCAGGGAG 
GTGACCGTGG AACACACCAC AAACATCCTC GAGGATCACT CTTTCACAGA GTTTATGGGT 
GTGTCAGATG GGAAAGATTT TGATGGTTTG ACGGATTCTT CTGCTGGAGA ACTTTCAAGT 
AGGAGGAACC TACCAAATAC AGCGGACAGT AGGAGTTTCT CACATGGGCC ACAGGACCCT 
GGCCAGCTGT GTTCCTCGGC AGAGAATGAC CAGGGGAGCG GCACTGCTCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_010818550.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816852.2 Bos taurus kinesin family member 13A (KIF13A), transcript variant X2, mRNA.

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CloneID	OBa229710
Clone ID Related Accession (Same CDS sequence)	XM_010818550.3
Accession Version	XM_010818550.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818550.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGATA CCAAGGTAAA AGTTGCCGTC CGGGTCCGGC CCCTGAACCG ACGAGAACTG 
GAACTCAACA CCAAGTGCGT GGTGGAGATG GAAGGGAATC AAACGGTCCT GCACCCTCCT 
CCTTCTAACA CCAAACAGGG AGAAAGGAAA CCTCCCAAGG TATTTGCATT TGATTATTGC 
TTTTGGTCCA TGGATGAATC CAACACTACA AAGTATGCTG GTCAAGAAGT GGTTTTCAAG 
TGCCTTGGGG AAGGAATTCT TGAAAAAGCC TTTCAGGGGT ATAATGCTTG CATTTTTGCA 
TATGGACAGA CAGGCTCAGG AAAATCCTTC TCCATGATGG GCAATGCTGA GCAGCGAGGC 
CTCATCCCAA GGCTCTGCTG TGCTTTATTT CAAAGGATCT CTCTGGAGCA GAATGAGTCA 
CAGACATTTA AAGTGGAAGT ATCCTATATG GAAATTTATA ATGAGAAAGT CCGGGACCTG 
TTAGACCCCA AAGGGAGTAG ACAGTCTCTT AAAGTTCGAG AGCATAAAGT TTTGGGACCA 
TATGTAGATG GTTTATCCCA ACTGGCTGTC ACTAGTTTTG AGGACATTGA GTCATTGATG 
TCTGAGGGAA ACAAATCTCG GACGGTAGCT GCGACCAACA TGAATGAAGA AAGCAGCCGC 
TCCCATGCAG TGTTCAACAT CGTCATCACG CAGACGCTGT ACGACCTGCA GTCTGGGAAC 
TCAGGGGAGA AGGTGAGCAA GGTGAGCTTG GTGGACCTGG CGGGCAGCGA GAGAGTGTCT 
AAAACTGGAG CTGCGGGAGA GCGCCTGAAA GAAGGCAGCA ACATTAACAA ATCACTTACA 
ACCTTGGGGT TGGTTATCTC ATCGCTGGCT GACCAGGCAG CTGGCAAGGG TAAAAACAAA 
TTTGTGCCTT ATCGCGACTC AGTCCTCACT TGGCTGCTTA AGGACAATCT GGGGGGCAAC 
AGCCAGACCT CCATGATCGC CACCATTAGC CCAGCAGCAG ACAACTACGA AGAGACCCTC 
TCCACGCTGA GATACGCAGA TCGGGCCAAA AGGATCGTTA ACCATGCCGT CGTGAATGAG 
GATCCCAACG CGAAGGTCAT CCGGGAACTG CGGGAAGAGG TGGAGAAACT GAGGGAGCAG 
CTCTCACAGG CTGAGGCTAT GAAGGCCCCT GAACTGAAAG AGAAGCTGGA AGAGTCTGAA 
AAGCTGATAA AAGAGCTAAC AGTGACTTGG GAAGAGAAGC TCAGGAAAAC AGAAGAAATT 
GCACAGGAGA GGCAGCGGCA ACTTGAAAGC ATGGGGATCT CCCTGGAGAC TTCTGGTATC 
AAAGTAGGAG ATGACAAGTG CTACTTGGTC AACCTGAATG CAGACCCTGC TCTCAACGAA 
CTTCTGGTTT ATTATTTAAA GGACCACACC AGGGTAGGTG CCGACACCTC TCAGGACATC 
CAGCTCTTTG GGATAGGAAT TCAGCCTGAG CACTGTGAGA TCGACATTGG CTCTGATGGA 
GAGGTCAGCC TCACTCCAAA AGAAAATGCA CGGTCCTGTG TCAATGGCAC CCTCGTGTGC 
AGCACCACCC AGCTGTGGCA TGGGGACCGA ATCCTGTGGG GAAACAACCA CTTCTTCAGG 
ATTAATTTGC CCAAGAGGAA ACGGCGAGAT TGGTTGAAAG ACTTTGAGAA AGAGACAGGC 
CCGCCGGAGC ACGACCTGGA CGCGGCCAGT GAAGCGTCCT CAGAACCAGA CTATAACTAC 
GAATTTGCGC AGATGGAGGT TATCATGAAA ACGCTGAATA GCAATGGTGG CTTCCCGATC 
ATAAGCCTGT CTGCCTGGTG GTCATCCCCT TGGTGGTCCT ATTTGAAGAT TCTCTTCCCT 
CTCATGTGGT TTCAGACGAA GCTCCTCCAC AAACACTGGG ATAAAGACCC AGTTCAGAAC 
GTGGTGCAGG TCTTGGAGAA ACAGTACCTG GAAGAAAAGC GAAGCGCCCT CGAGGAGCAG 
CGACTCATGT ACGAGCGGGA GCTGGAGCAG CTCCGCCAGC AGCTGTCCCC GGAGAGGCAG 
CCCCAGATCA GCGGCCCCGA CCGCCTGGCC TACAGCAGCC AGACGGCGCA GCAGAAGGTG 
ACCCAGTGGG CCGAGGAGAG GGACGAACTC TTTCGCCAAA GTCTGGCAAA ACTTCGGGAG 
CAGCTGGTGA AAGCCAACAC CCTGGTGAGA GAGGCAAACT TCTTAGCCGA GGAGATGAGC 
AAGCTCACCG ACTACCAGGT GACTCTGCAG ATCCCTGCTG CGAACCTCAG TGCCAACAGG 
AAGAGAGGAG CAATAGTGAG TGAGCCAGCT ATTCAAGTGA GGAGGAAAGG AAAGAGCACC 
CAAGTGTGGA CCATTGAGAA GCTGGAGAAT AAATTAATTG ATATGAGAGA CCTTTATCAA 
GAATGGAAAG AAAAGATTCC TGAGGCAAAG CGACTCTGCG GGAAACGAGG TGACCCTTTC 
TATGAAGCCC AAGAGAATCA CAACCTGATT GGCGTGGCGA ATGTGTTCCT GGAGTGTCTC 
TTCTGTGATG TGAAGCTTCA GTATGCAGTT CCTATCATCA GCCAGCAGGG GGAGGTTGCA 
GGGCGTCTCC ACGTGGAAGT GATGCGAGTG ACAGGCGCTG TCCCGGAGCG CATGGCGGAG 
GACGACTCCT CGGAGAACTC CAGTGAAAGC GGAAGTCTTG AAGTCGTAGA CAGCACCGGG 
GAAATCATTC ACCGAGTCAA GAAGCTGACA TGCCGGGTAA AAATTAAAGA AGCAACTGGG 
CTGCCCTTAA ACCTCTCAAA TTTCGTCTTC TGTCAATACA CGTTCTGGGA CCAGTGTGAG 
CCCACGGTGG CCGCCCCTGT CGTGGATCCT GAGGTGCCTT CCCCACAGTC CAAGGACGCC 
CAGTACACCG TGACCTTCTC CCACTGTCAG GATTACGTGG TCAACGTAAC GGAAGAATTC 
CTGGAGTTCA TTTCAGATGG AGCACTGGCC ATCGAAGTGT GGGGCCACCG GTGTACCGGG 
AACGGCAGCT CCATCTGGGA AGTCGATTCC CTTCATGCTA AGACGAGAAC GCTGCATGAC 
AGGTGGAATG AAGTTACTCG CAGAATAGAG ATGTGGATCT CCATCCTGGA GCTGAACGAG 
CTGGGGGAGT ACGCCGCCGT GGAGCTTCAC CAGGCCAAGG ACGTCAACAC AGGCGGCGTC 
CTTCAGCTCA GACAGGGTCA TTCCCGGAGA GTCCAAGTCA CAGTGAAGCC TGTCCAGCAT 
TCCGGGACAC TGCCACTCAT GGTTGAAGCC ATCTTGTCCA TCTCCATCGG CTGCATCACT 
GCCAGGTCAA CCAAGCTCCA GAGAGGGCTG GACAGTTACC AGAGAGATGA TGAGGATGGT 
GATGATATGG ATAGTTATCA GGAAGAAGAC TTAAACTGCG TGAGAGAGAG GTGGTCGGAT 
GCCCTCATTA AACGGCGAGA ATACCTAGAC GAACAGATTA AAAAAGTCAG CAACAAAAAA 
GAGAAGACGG AGGATGACGT GGAGCGGGAA GCCCGGCTGG TGGAGCAGTG GGTGGGGCTG 
ACGGAGGAGA GGAACGCTGT GCTGGTGCCT GCGCCGGGCA GCGGGATCCC CGGGGCGCCT 
GCCGACTGGA TCCCACCACC TGGAATGGAA ACCCACATAC CGGTTCTCTT CCTTGATTTG 
AACGCGGATG ACCTCAGTGC CAATGAGCAG CTCGTGGGCC CCCACGCATC AGGCGTGAAC 
TCCATCCTCC CCAAGGAGCA CGGCAGCCAG TTTTTCTACC TGCCCATCAT AAAGCACAGT 
GACGAGGAGG TTTCAGCCAC AGCCTCCTGG GACTCCTCGG TGCATGATTC CGTTCACTTG 
AACAGGGTCA CCCCGCAGAA TGAGAGGATC TACCTGATAG TGAAGACCAC AGTCCAGCTC 
AGCCACCCGG CCGCTATGGA GCTGGTCTTA CGAAAACGGA TTGCAGCCAA CATTTACAAC 
AAACAGAGTT TCACCCAGAG TTTGAAGAGG AGAATATCAC TGAAGAATAT ATTTTATTCC 
TGTGGTGTAA CCTATGAAAT AGTATCCAAC ATACCAAAGG CAACAGAGGA GATTGAGGAC 
CGGGAGACGC TGGCTCTCAT GGCTGCAAGG AGTGAGAATG AAGGCACGTC GGATGGGGAG 
ACGTACATCG AGAAGTACAC GCGGGGCGTG CTGCAGGTGG AGAACATTCT GAGCCTGGAA 
CGACTCCGGC AGGCGGTGAC GGTCAAAGAA GCACTTTCCA CCAAAGCTCG GCACCTACGG 
CGAAGCCTCA GCACGCCCAA CGTTCACAAT GTCTCTTCCA GTCGACCAGA CCTTTCTGGC 
TTTGACGATG ATGATAAGGG TTGGCCAGAG AACCAGTTAG ACATGTCTGA CTACAGCCCG 
AGTTACCAGG ATGTATCGTG TTATGGAACT TTGCCTAGGG ATTCACCTCG AAGGAGTAAA 
GAAGGTTGTA CATCAGAGAA TCCTCATGCC TTAACAGTCA GCCCTTTCAA AGCATTCTCT 
CCTCAGCCAC CAAAGTTTTT CAAGCCCCTA ATGCCTGTAA AAGAGGAGCA TAAGAAAAGG 
ATAGCGCTTG AAGCAAGGCC TCTTCTAAGC CAGGAGATTC TGAAGTTAAT CTACCTTGAG 
GAATTGCACT TACTTGGAGA CTCTGAGGAG GAGGAAAATG ATCTAGAAGG TCTCAGCCGG 
AAGCTGATAA GTTCACAGCC TTATGTACCT GTGGAGTTCG CTGACTTCAG CGTTTACAAT 
GCCAGCTTGG AGAACAGAGA GTGGTTTTCT TCTAAAGGCG ATCTGACGAA CTCAAAGGTC 
CTAGAGAAGG AGGTGTCCCG CAGCCCCACC ACCAGCAGCA TCACCAGCGG CTACTTTTCC 
CACAGTGCCT CCAACGCCAC CCTGTCCGAC ATGGTGGTCC CTTCCAGTGA CAGCTCAGAT 
CAGTTGGCCC TTCCAACGAA GGACACAGAT TCCAGCGAGC ATCCCGGACC GTCCCTTGGG 
CAGGATTTCA GACCATCTTC AAACAAAGAG TTGACAGAAC TAGAAAGAGG CTTGGGAAAG 
GATAAGATGA CCGTGGTGCC ACTCAAGGAA AACAGTGCCT TAGCCAAAGG GAGCCCATTG 
TCCCCCAGTA TCCCGGAGAA AAACTCCAGA ACCCTCTGTA GGACCAGCTC GTGTTCAGAG 
CAGGATGCCT GCTCCAGCAA AAACGGCCAG CCGGCCAGGG AATTCTGCCC CAGGGAGGTG 
ACCGTGGAAC ACACCACAAA CATCCTCGAG GATCACTCTT TCACAGAGTT TATGGGTGTG 
TCAGATGGGA AAGATTTTGA TGGTTTGACG GATTCTTCTG CTGGAGAACT TTCAAGTAGG 
AGGAACCTAC CAAATACAGC GGACAGTAGG AGTTTCTCAC ATGGGCCACA GGACCCTGGC 
CAGCTGTGTT CCTCGGCAGA GAATGACCAG GGGAGCGGCA CTGCTCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816852.2
CDS	89..5539
Translation	MSDTKVKVAVRVRPLNRRELELNTKCVVEMEGNQTVLHPPPSNTKQGERKPPKVFAFDYCFWSMDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQEILKLIYLEELHLLGDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_010818550.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 13A (KIF13A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818550.3

ORF Insert Sequence:

ATGTCGGATA CCAAGGTAAA AGTTGCCGTC CGGGTCCGGC CCCTGAACCG ACGAGAACTG 
GAACTCAACA CCAAGTGCGT GGTGGAGATG GAAGGGAATC AAACGGTCCT GCACCCTCCT 
CCTTCTAACA CCAAACAGGG AGAAAGGAAA CCTCCCAAGG TATTTGCATT TGATTATTGC 
TTTTGGTCCA TGGATGAATC CAACACTACA AAGTATGCTG GTCAAGAAGT GGTTTTCAAG 
TGCCTTGGGG AAGGAATTCT TGAAAAAGCC TTTCAGGGGT ATAATGCTTG CATTTTTGCA 
TATGGACAGA CAGGCTCAGG AAAATCCTTC TCCATGATGG GCAATGCTGA GCAGCGAGGC 
CTCATCCCAA GGCTCTGCTG TGCTTTATTT CAAAGGATCT CTCTGGAGCA GAATGAGTCA 
CAGACATTTA AAGTGGAAGT ATCCTATATG GAAATTTATA ATGAGAAAGT CCGGGACCTG 
TTAGACCCCA AAGGGAGTAG ACAGTCTCTT AAAGTTCGAG AGCATAAAGT TTTGGGACCA 
TATGTAGATG GTTTATCCCA ACTGGCTGTC ACTAGTTTTG AGGACATTGA GTCATTGATG 
TCTGAGGGAA ACAAATCTCG GACGGTAGCT GCGACCAACA TGAATGAAGA AAGCAGCCGC 
TCCCATGCAG TGTTCAACAT CGTCATCACG CAGACGCTGT ACGACCTGCA GTCTGGGAAC 
TCAGGGGAGA AGGTGAGCAA GGTGAGCTTG GTGGACCTGG CGGGCAGCGA GAGAGTGTCT 
AAAACTGGAG CTGCGGGAGA GCGCCTGAAA GAAGGCAGCA ACATTAACAA ATCACTTACA 
ACCTTGGGGT TGGTTATCTC ATCGCTGGCT GACCAGGCAG CTGGCAAGGG TAAAAACAAA 
TTTGTGCCTT ATCGCGACTC AGTCCTCACT TGGCTGCTTA AGGACAATCT GGGGGGCAAC 
AGCCAGACCT CCATGATCGC CACCATTAGC CCAGCAGCAG ACAACTACGA AGAGACCCTC 
TCCACGCTGA GATACGCAGA TCGGGCCAAA AGGATCGTTA ACCATGCCGT CGTGAATGAG 
GATCCCAACG CGAAGGTCAT CCGGGAACTG CGGGAAGAGG TGGAGAAACT GAGGGAGCAG 
CTCTCACAGG CTGAGGCTAT GAAGGCCCCT GAACTGAAAG AGAAGCTGGA AGAGTCTGAA 
AAGCTGATAA AAGAGCTAAC AGTGACTTGG GAAGAGAAGC TCAGGAAAAC AGAAGAAATT 
GCACAGGAGA GGCAGCGGCA ACTTGAAAGC ATGGGGATCT CCCTGGAGAC TTCTGGTATC 
AAAGTAGGAG ATGACAAGTG CTACTTGGTC AACCTGAATG CAGACCCTGC TCTCAACGAA 
CTTCTGGTTT ATTATTTAAA GGACCACACC AGGGTAGGTG CCGACACCTC TCAGGACATC 
CAGCTCTTTG GGATAGGAAT TCAGCCTGAG CACTGTGAGA TCGACATTGG CTCTGATGGA 
GAGGTCAGCC TCACTCCAAA AGAAAATGCA CGGTCCTGTG TCAATGGCAC CCTCGTGTGC 
AGCACCACCC AGCTGTGGCA TGGGGACCGA ATCCTGTGGG GAAACAACCA CTTCTTCAGG 
ATTAATTTGC CCAAGAGGAA ACGGCGAGAT TGGTTGAAAG ACTTTGAGAA AGAGACAGGC 
CCGCCGGAGC ACGACCTGGA CGCGGCCAGT GAAGCGTCCT CAGAACCAGA CTATAACTAC 
GAATTTGCGC AGATGGAGGT TATCATGAAA ACGCTGAATA GCAATGGTGG CTTCCCGATC 
ATAAGCCTGT CTGCCTGGTG GTCATCCCCT TGGTGGTCCT ATTTGAAGAT TCTCTTCCCT 
CTCATGTGGT TTCAGACGAA GCTCCTCCAC AAACACTGGG ATAAAGACCC AGTTCAGAAC 
GTGGTGCAGG TCTTGGAGAA ACAGTACCTG GAAGAAAAGC GAAGCGCCCT CGAGGAGCAG 
CGACTCATGT ACGAGCGGGA GCTGGAGCAG CTCCGCCAGC AGCTGTCCCC GGAGAGGCAG 
CCCCAGATCA GCGGCCCCGA CCGCCTGGCC TACAGCAGCC AGACGGCGCA GCAGAAGGTG 
ACCCAGTGGG CCGAGGAGAG GGACGAACTC TTTCGCCAAA GTCTGGCAAA ACTTCGGGAG 
CAGCTGGTGA AAGCCAACAC CCTGGTGAGA GAGGCAAACT TCTTAGCCGA GGAGATGAGC 
AAGCTCACCG ACTACCAGGT GACTCTGCAG ATCCCTGCTG CGAACCTCAG TGCCAACAGG 
AAGAGAGGAG CAATAGTGAG TGAGCCAGCT ATTCAAGTGA GGAGGAAAGG AAAGAGCACC 
CAAGTGTGGA CCATTGAGAA GCTGGAGAAT AAATTAATTG ATATGAGAGA CCTTTATCAA 
GAATGGAAAG AAAAGATTCC TGAGGCAAAG CGACTCTGCG GGAAACGAGG TGACCCTTTC 
TATGAAGCCC AAGAGAATCA CAACCTGATT GGCGTGGCGA ATGTGTTCCT GGAGTGTCTC 
TTCTGTGATG TGAAGCTTCA GTATGCAGTT CCTATCATCA GCCAGCAGGG GGAGGTTGCA 
GGGCGTCTCC ACGTGGAAGT GATGCGAGTG ACAGGCGCTG TCCCGGAGCG CATGGCGGAG 
GACGACTCCT CGGAGAACTC CAGTGAAAGC GGAAGTCTTG AAGTCGTAGA CAGCACCGGG 
GAAATCATTC ACCGAGTCAA GAAGCTGACA TGCCGGGTAA AAATTAAAGA AGCAACTGGG 
CTGCCCTTAA ACCTCTCAAA TTTCGTCTTC TGTCAATACA CGTTCTGGGA CCAGTGTGAG 
CCCACGGTGG CCGCCCCTGT CGTGGATCCT GAGGTGCCTT CCCCACAGTC CAAGGACGCC 
CAGTACACCG TGACCTTCTC CCACTGTCAG GATTACGTGG TCAACGTAAC GGAAGAATTC 
CTGGAGTTCA TTTCAGATGG AGCACTGGCC ATCGAAGTGT GGGGCCACCG GTGTACCGGG 
AACGGCAGCT CCATCTGGGA AGTCGATTCC CTTCATGCTA AGACGAGAAC GCTGCATGAC 
AGGTGGAATG AAGTTACTCG CAGAATAGAG ATGTGGATCT CCATCCTGGA GCTGAACGAG 
CTGGGGGAGT ACGCCGCCGT GGAGCTTCAC CAGGCCAAGG ACGTCAACAC AGGCGGCGTC 
CTTCAGCTCA GACAGGGTCA TTCCCGGAGA GTCCAAGTCA CAGTGAAGCC TGTCCAGCAT 
TCCGGGACAC TGCCACTCAT GGTTGAAGCC ATCTTGTCCA TCTCCATCGG CTGCATCACT 
GCCAGGTCAA CCAAGCTCCA GAGAGGGCTG GACAGTTACC AGAGAGATGA TGAGGATGGT 
GATGATATGG ATAGTTATCA GGAAGAAGAC TTAAACTGCG TGAGAGAGAG GTGGTCGGAT 
GCCCTCATTA AACGGCGAGA ATACCTAGAC GAACAGATTA AAAAAGTCAG CAACAAAAAA 
GAGAAGACGG AGGATGACGT GGAGCGGGAA GCCCGGCTGG TGGAGCAGTG GGTGGGGCTG 
ACGGAGGAGA GGAACGCTGT GCTGGTGCCT GCGCCGGGCA GCGGGATCCC CGGGGCGCCT 
GCCGACTGGA TCCCACCACC TGGAATGGAA ACCCACATAC CGGTTCTCTT CCTTGATTTG 
AACGCGGATG ACCTCAGTGC CAATGAGCAG CTCGTGGGCC CCCACGCATC AGGCGTGAAC 
TCCATCCTCC CCAAGGAGCA CGGCAGCCAG TTTTTCTACC TGCCCATCAT AAAGCACAGT 
GACGAGGAGG TTTCAGCCAC AGCCTCCTGG GACTCCTCGG TGCATGATTC CGTTCACTTG 
AACAGGGTCA CCCCGCAGAA TGAGAGGATC TACCTGATAG TGAAGACCAC AGTCCAGCTC 
AGCCACCCGG CCGCTATGGA GCTGGTCTTA CGAAAACGGA TTGCAGCCAA CATTTACAAC 
AAACAGAGTT TCACCCAGAG TTTGAAGAGG AGAATATCAC TGAAGAATAT ATTTTATTCC 
TGTGGTGTAA CCTATGAAAT AGTATCCAAC ATACCAAAGG CAACAGAGGA GATTGAGGAC 
CGGGAGACGC TGGCTCTCAT GGCTGCAAGG AGTGAGAATG AAGGCACGTC GGATGGGGAG 
ACGTACATCG AGAAGTACAC GCGGGGCGTG CTGCAGGTGG AGAACATTCT GAGCCTGGAA 
CGACTCCGGC AGGCGGTGAC GGTCAAAGAA GCACTTTCCA CCAAAGCTCG GCACCTACGG 
CGAAGCCTCA GCACGCCCAA CGTTCACAAT GTCTCTTCCA GTCGACCAGA CCTTTCTGGC 
TTTGACGATG ATGATAAGGG TTGGCCAGAG AACCAGTTAG ACATGTCTGA CTACAGCCCG 
AGTTACCAGG ATGTATCGTG TTATGGAACT TTGCCTAGGG ATTCACCTCG AAGGAGTAAA 
GAAGGTTGTA CATCAGAGAA TCCTCATGCC TTAACAGTCA GCCCTTTCAA AGCATTCTCT 
CCTCAGCCAC CAAAGTTTTT CAAGCCCCTA ATGCCTGTAA AAGAGGAGCA TAAGAAAAGG 
ATAGCGCTTG AAGCAAGGCC TCTTCTAAGC CAGGAGATTC TGAAGTTAAT CTACCTTGAG 
GAATTGCACT TACTTGGAGA CTCTGAGGAG GAGGAAAATG ATCTAGAAGG TCTCAGCCGG 
AAGCTGATAA GTTCACAGCC TTATGTACCT GTGGAGTTCG CTGACTTCAG CGTTTACAAT 
GCCAGCTTGG AGAACAGAGA GTGGTTTTCT TCTAAAGGCG ATCTGACGAA CTCAAAGGTC 
CTAGAGAAGG AGGTGTCCCG CAGCCCCACC ACCAGCAGCA TCACCAGCGG CTACTTTTCC 
CACAGTGCCT CCAACGCCAC CCTGTCCGAC ATGGTGGTCC CTTCCAGTGA CAGCTCAGAT 
CAGTTGGCCC TTCCAACGAA GGACACAGAT TCCAGCGAGC ATCCCGGACC GTCCCTTGGG 
CAGGATTTCA GACCATCTTC AAACAAAGAG TTGACAGAAC TAGAAAGAGG CTTGGGAAAG 
GATAAGATGA CCGTGGTGCC ACTCAAGGAA AACAGTGCCT TAGCCAAAGG GAGCCCATTG 
TCCCCCAGTA TCCCGGAGAA AAACTCCAGA ACCCTCTGTA GGACCAGCTC GTGTTCAGAG 
CAGGATGCCT GCTCCAGCAA AAACGGCCAG CCGGCCAGGG AATTCTGCCC CAGGGAGGTG 
ACCGTGGAAC ACACCACAAA CATCCTCGAG GATCACTCTT TCACAGAGTT TATGGGTGTG 
TCAGATGGGA AAGATTTTGA TGGTTTGACG GATTCTTCTG CTGGAGAACT TTCAAGTAGG 
AGGAACCTAC CAAATACAGC GGACAGTAGG AGTTTCTCAC ATGGGCCACA GGACCCTGGC 
CAGCTGTGTT CCTCGGCAGA GAATGACCAG GGGAGCGGCA CTGCTCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_010818552.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816854.2 Bos taurus kinesin family member 13A (KIF13A), transcript variant X3, mRNA.

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CloneID	OBa229711
Clone ID Related Accession (Same CDS sequence)	XM_010818552.3
Accession Version	XM_010818552.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818552.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGATA CCAAGGTAAA AGTTGCCGTC CGGGTCCGGC CCCTGAACCG ACGAGAACTG 
GAACTCAACA CCAAGTGCGT GGTGGAGATG GAAGGGAATC AAACGGTCCT GCACCCTCCT 
CCTTCTAACA CCAAACAGGG AGAAAGGAAA CCTCCCAAGG TATTTGCATT TGATTATTGC 
TTTTGGTCCA TGGATGAATC CAACACTACA AAGTATGCTG GTCAAGAAGT GGTTTTCAAG 
TGCCTTGGGG AAGGAATTCT TGAAAAAGCC TTTCAGGGGT ATAATGCTTG CATTTTTGCA 
TATGGACAGA CAGGCTCAGG AAAATCCTTC TCCATGATGG GCAATGCTGA GCAGCGAGGC 
CTCATCCCAA GGCTCTGCTG TGCTTTATTT CAAAGGATCT CTCTGGAGCA GAATGAGTCA 
CAGACATTTA AAGTGGAAGT ATCCTATATG GAAATTTATA ATGAGAAAGT CCGGGACCTG 
TTAGACCCCA AAGGGAGTAG ACAGTCTCTT AAAGTTCGAG AGCATAAAGT TTTGGGACCA 
TATGTAGATG GTTTATCCCA ACTGGCTGTC ACTAGTTTTG AGGACATTGA GTCATTGATG 
TCTGAGGGAA ACAAATCTCG GACGGTAGCT GCGACCAACA TGAATGAAGA AAGCAGCCGC 
TCCCATGCAG TGTTCAACAT CGTCATCACG CAGACGCTGT ACGACCTGCA GTCTGGGAAC 
TCAGGGGAGA AGGTGAGCAA GGTGAGCTTG GTGGACCTGG CGGGCAGCGA GAGAGTGTCT 
AAAACTGGAG CTGCGGGAGA GCGCCTGAAA GAAGGCAGCA ACATTAACAA ATCACTTACA 
ACCTTGGGGT TGGTTATCTC ATCGCTGGCT GACCAGGCAG CTGGCAAGGG TAAAAACAAA 
TTTGTGCCTT ATCGCGACTC AGTCCTCACT TGGCTGCTTA AGGACAATCT GGGGGGCAAC 
AGCCAGACCT CCATGATCGC CACCATTAGC CCAGCAGCAG ACAACTACGA AGAGACCCTC 
TCCACGCTGA GATACGCAGA TCGGGCCAAA AGGATCGTTA ACCATGCCGT CGTGAATGAG 
GATCCCAACG CGAAGGTCAT CCGGGAACTG CGGGAAGAGG TGGAGAAACT GAGGGAGCAG 
CTCTCACAGG CTGAGGCTAT GAAGGCCCCT GAACTGAAAG AGAAGCTGGA AGAGTCTGAA 
AAGCTGATAA AAGAGCTAAC AGTGACTTGG GAAGAGAAGC TCAGGAAAAC AGAAGAAATT 
GCACAGGAGA GGCAGCGGCA ACTTGAAAGC ATGGGGATCT CCCTGGAGAC TTCTGGTATC 
AAAGTAGGAG ATGACAAGTG CTACTTGGTC AACCTGAATG CAGACCCTGC TCTCAACGAA 
CTTCTGGTTT ATTATTTAAA GGACCACACC AGGGTAGGTG CCGACACCTC TCAGGACATC 
CAGCTCTTTG GGATAGGAAT TCAGCCTGAG CACTGTGAGA TCGACATTGG CTCTGATGGA 
GAGGTCAGCC TCACTCCAAA AGAAAATGCA CGGTCCTGTG TCAATGGCAC CCTCGTGTGC 
AGCACCACCC AGCTGTGGCA TGGGGACCGA ATCCTGTGGG GAAACAACCA CTTCTTCAGG 
ATTAATTTGC CCAAGAGGAA ACGGCGAGAT TGGTTGAAAG ACTTTGAGAA AGAGACAGGC 
CCGCCGGAGC ACGACCTGGA CGCGGCCAGT GAAGCGTCCT CAGAACCAGA CTATAACTAC 
GAATTTGCGC AGATGGAGGT TATCATGAAA ACGCTGAATA GCAATGGTGG CTTCCCGATC 
ATAAGCCTGT CTGCCTGGTG GTCATCCCCT TGGTGGTCCT ATTTGAAGAT TCTCTTCCCT 
CTCATGTGGT TTCAGACGAA GCTCCTCCAC AAACACTGGG ATAAAGACCC AGTTCAGAAC 
GTGGTGCAGG TCTTGGAGAA ACAGTACCTG GAAGAAAAGC GAAGCGCCCT CGAGGAGCAG 
CGACTCATGT ACGAGCGGGA GCTGGAGCAG CTCCGCCAGC AGCTGTCCCC GGAGAGGCAG 
CCCCAGATCA GCGGCCCCGA CCGCCTGGCC TACAGCAGCC AGACGGCGCA GCAGAAGGTG 
ACCCAGTGGG CCGAGGAGAG GGACGAACTC TTTCGCCAAA GTCTGGCAAA ACTTCGGGAG 
CAGCTGGTGA AAGCCAACAC CCTGGTGAGA GAGGCAAACT TCTTAGCCGA GGAGATGAGC 
AAGCTCACCG ACTACCAGGT GACTCTGCAG ATCCCTGCTG CGAACCTCAG TGCCAACAGG 
AAGAGAGGAG CAATAGTGAG TGAGCCAGCT ATTCAAGTGA GGAGGAAAGG AAAGAGCACC 
CAAGTGTGGA CCATTGAGAA GCTGGAGAAT AAATTAATTG ATATGAGAGA CCTTTATCAA 
GAATGGAAAG AAAAGATTCC TGAGGCAAAG CGACTCTGCG GGAAACGAGG TGACCCTTTC 
TATGAAGCCC AAGAGAATCA CAACCTGATT GGCGTGGCGA ATGTGTTCCT GGAGTGTCTC 
TTCTGTGATG TGAAGCTTCA GTATGCAGTT CCTATCATCA GCCAGCAGGG GGAGGTTGCA 
GGGCGTCTCC ACGTGGAAGT GATGCGAGTG ACAGGCGCTG TCCCGGAGCG CATGGCGGAG 
GACGACTCCT CGGAGAACTC CAGTGAAAGC GGAAGTCTTG AAGTCGTAGA CAGCACCGGG 
GAAATCATTC ACCGAGTCAA GAAGCTGACA TGCCGGGTAA AAATTAAAGA AGCAACTGGG 
CTGCCCTTAA ACCTCTCAAA TTTCGTCTTC TGTCAATACA CGTTCTGGGA CCAGTGTGAG 
CCCACGGTGG CCGCCCCTGT CGTGGATCCT GAGGTGCCTT CCCCACAGTC CAAGGACGCC 
CAGTACACCG TGACCTTCTC CCACTGTCAG GATTACGTGG TCAACGTAAC GGAAGAATTC 
CTGGAGTTCA TTTCAGATGG AGCACTGGCC ATCGAAGTGT GGGGCCACCG GTGTACCGGG 
AACGGCAGCT CCATCTGGGA AGTCGATTCC CTTCATGCTA AGACGAGAAC GCTGCATGAC 
AGGTGGAATG AAGTTACTCG CAGAATAGAG ATGTGGATCT CCATCCTGGA GCTGAACGAG 
CTGGGGGAGT ACGCCGCCGT GGAGCTTCAC CAGGCCAAGG ACGTCAACAC AGGCGGCGTC 
CTTCAGCTCA GACAGGGTCA TTCCCGGAGA GTCCAAGTCA CAGTGAAGCC TGTCCAGCAT 
TCCGGGACAC TGCCACTCAT GGTTGAAGCC ATCTTGTCCA TCTCCATCGG CTGCATCACT 
GCCAGGTCAA CCAAGCTCCA GAGAGGGCTG GACAGTTACC AGAGAGATGA TGAGGATGGT 
GATGATATGG ATAGTTATCA GGAAGAAGAC TTAAACTGCG TGAGAGAGAG GTGGTCGGAT 
GCCCTCATTA AACGGCGAGA ATACCTAGAC GAACAGATTA AAAAAGTCAG CAACAAAAAA 
GAGAAGACGG AGGATGACGT GGAGCGGGAA GCCCGGCTGG TGGAGCAGTG GGTGGGGCTG 
ACGGAGGAGA GGAACGCTGT GCTGGTGCCT GCGCCGGGCA GCGGGATCCC CGGGGCGCCT 
GCCGACTGGA TCCCACCACC TGGAATGGAA ACCCACATAC CGGTTCTCTT CCTTGATTTG 
AACGCGGATG ACCTCAGTGC CAATGAGCAG CTCGTGGGCC CCCACGCATC AGGCGTGAAC 
TCCATCCTCC CCAAGGAGCA CGGCAGCCAG TTTTTCTACC TGCCCATCAT AAAGCACAGT 
GACGAGGAGG TTTCAGCCAC AGCCTCCTGG GACTCCTCGG TGCATGATTC CGTTCACTTG 
AACAGGGTCA CCCCGCAGAA TGAGAGGATC TACCTGATAG TGAAGACCAC AGTCCAGCTC 
AGCCACCCGG CCGCTATGGA GCTGGTCTTA CGAAAACGGA TTGCAGCCAA CATTTACAAC 
AAACAGAGTT TCACCCAGAG TTTGAAGAGG AGAATATCAC TGAAGAATAT ATTTTATTCC 
TGTGGTGTAA CCTATGAAAT AGTATCCAAC ATACCAAAGG CAACAGAGGA GATTGAGGAC 
CGGGAGACGC TGGCTCTCAT GGCTGCAAGG AGTGAGAATG AAGGCACGTC GGATGGGGAG 
ACGTACATCG AGAAGTACAC GCGGGGCGTG CTGCAGGTGG AGAACATTCT GAGCCTGGAA 
CGACTCCGGC AGGCGGTGAC GGTCAAAGAA GCACTTTCCA CCAAAGCTCG GCACCTACGG 
CGAAGCCTCA GCACGCCCAA CGTTCACAAT GTCTCTTCCA GTCGACCAGA CCTTTCTGGC 
TTTGACGATG ATGATAAGGG TTGGCCAGAG AACCAGTTAG ACATGTCTGA CTACAGCCCG 
AGTTACCAGG ATGTATCGTG TTATGGAACT TTGCCTAGGG ATTCACCTCG AAGGAGTAAA 
GAAGGTTGTA CATCAGAGAA TCCTCATGCC TTAACAGTCA GCCCTTTCAA AGCATTCTCT 
CCTCAGCCAC CAAAGTTTTT CAAGCCCCTA ATGCCTGTAA AAGAGGAGCA TAAGAAAAGG 
ATAGCGCTTG AAGCAAGGCC TCTTCTAAGC CAGGAGGACT CTGAGGAGGA GGAAAATGAT 
CTAGAAGGTC TCAGCCGGAA GCTGATAAGT TCACAGCCTT ATGTACCTGT GGAGTTCGCT 
GACTTCAGCG TTTACAATGC CAGCTTGGAG AACAGAGAGT GGTTTTCTTC TAAAGGCGAT 
CTGACGAACT CAAAGGTCCT AGAGAAGGAG GTGTCCCGCA GCCCCACCAC CAGCAGCATC 
ACCAGCGGCT ACTTTTCCCA CAGTGCCTCC AACGCCACCC TGTCCGACAT GGTGGTCCCT 
TCCAGTGACA GCTCAGATCA GTTGGCCCTT CCAACGAAGG ACACAGATTC CAGCGAGCAT 
CCCGGACCGT CCCTTGGGCA GGATTTCAGA CCATCTTCAA ACAAAGAGTT GACAGAACTA 
GAAAGAGGCT TGGGAAAGGA TAAGATGACC GTGGTGCCAC TCAAGGAAAA CAGTGCCTTA 
GCCAAAGGGA GCCCATTGTC CCCCAGTATC CCGGAGAAAA ACTCCAGAAC CCTCTGTAGG 
ACCAGCTCGT GTTCAGAGCA GGATGCCTGC TCCAGCAAAA ACGGCCAGCC GGCCAGGGAA 
TTCTGCCCCA GGGAGGTGAC CGTGGAACAC ACCACAAACA TCCTCGAGGA TCACTCTTTC 
ACAGAGTTTA TGGGTGTGTC AGATGGGAAA GATTTTGATG GTTTGACGGA TTCTTCTGCT 
GGAGAACTTT CAAGTAGGAG GAACCTACCA AATACAGCGG ACAGTAGGAG TTTCTCACAT 
GGGCCACAGG ACCCTGGCCA GCTGTGTTCC TCGGCAGAGA ATGACCAGGG GAGCGGCACT 
GCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816854.2
CDS	89..5497
Translation	MSDTKVKVAVRVRPLNRRELELNTKCVVEMEGNQTVLHPPPSNTKQGERKPPKVFAFDYCFWSMDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQEDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_010818552.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 13A (KIF13A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818552.3

ORF Insert Sequence:

ATGTCGGATA CCAAGGTAAA AGTTGCCGTC CGGGTCCGGC CCCTGAACCG ACGAGAACTG 
GAACTCAACA CCAAGTGCGT GGTGGAGATG GAAGGGAATC AAACGGTCCT GCACCCTCCT 
CCTTCTAACA CCAAACAGGG AGAAAGGAAA CCTCCCAAGG TATTTGCATT TGATTATTGC 
TTTTGGTCCA TGGATGAATC CAACACTACA AAGTATGCTG GTCAAGAAGT GGTTTTCAAG 
TGCCTTGGGG AAGGAATTCT TGAAAAAGCC TTTCAGGGGT ATAATGCTTG CATTTTTGCA 
TATGGACAGA CAGGCTCAGG AAAATCCTTC TCCATGATGG GCAATGCTGA GCAGCGAGGC 
CTCATCCCAA GGCTCTGCTG TGCTTTATTT CAAAGGATCT CTCTGGAGCA GAATGAGTCA 
CAGACATTTA AAGTGGAAGT ATCCTATATG GAAATTTATA ATGAGAAAGT CCGGGACCTG 
TTAGACCCCA AAGGGAGTAG ACAGTCTCTT AAAGTTCGAG AGCATAAAGT TTTGGGACCA 
TATGTAGATG GTTTATCCCA ACTGGCTGTC ACTAGTTTTG AGGACATTGA GTCATTGATG 
TCTGAGGGAA ACAAATCTCG GACGGTAGCT GCGACCAACA TGAATGAAGA AAGCAGCCGC 
TCCCATGCAG TGTTCAACAT CGTCATCACG CAGACGCTGT ACGACCTGCA GTCTGGGAAC 
TCAGGGGAGA AGGTGAGCAA GGTGAGCTTG GTGGACCTGG CGGGCAGCGA GAGAGTGTCT 
AAAACTGGAG CTGCGGGAGA GCGCCTGAAA GAAGGCAGCA ACATTAACAA ATCACTTACA 
ACCTTGGGGT TGGTTATCTC ATCGCTGGCT GACCAGGCAG CTGGCAAGGG TAAAAACAAA 
TTTGTGCCTT ATCGCGACTC AGTCCTCACT TGGCTGCTTA AGGACAATCT GGGGGGCAAC 
AGCCAGACCT CCATGATCGC CACCATTAGC CCAGCAGCAG ACAACTACGA AGAGACCCTC 
TCCACGCTGA GATACGCAGA TCGGGCCAAA AGGATCGTTA ACCATGCCGT CGTGAATGAG 
GATCCCAACG CGAAGGTCAT CCGGGAACTG CGGGAAGAGG TGGAGAAACT GAGGGAGCAG 
CTCTCACAGG CTGAGGCTAT GAAGGCCCCT GAACTGAAAG AGAAGCTGGA AGAGTCTGAA 
AAGCTGATAA AAGAGCTAAC AGTGACTTGG GAAGAGAAGC TCAGGAAAAC AGAAGAAATT 
GCACAGGAGA GGCAGCGGCA ACTTGAAAGC ATGGGGATCT CCCTGGAGAC TTCTGGTATC 
AAAGTAGGAG ATGACAAGTG CTACTTGGTC AACCTGAATG CAGACCCTGC TCTCAACGAA 
CTTCTGGTTT ATTATTTAAA GGACCACACC AGGGTAGGTG CCGACACCTC TCAGGACATC 
CAGCTCTTTG GGATAGGAAT TCAGCCTGAG CACTGTGAGA TCGACATTGG CTCTGATGGA 
GAGGTCAGCC TCACTCCAAA AGAAAATGCA CGGTCCTGTG TCAATGGCAC CCTCGTGTGC 
AGCACCACCC AGCTGTGGCA TGGGGACCGA ATCCTGTGGG GAAACAACCA CTTCTTCAGG 
ATTAATTTGC CCAAGAGGAA ACGGCGAGAT TGGTTGAAAG ACTTTGAGAA AGAGACAGGC 
CCGCCGGAGC ACGACCTGGA CGCGGCCAGT GAAGCGTCCT CAGAACCAGA CTATAACTAC 
GAATTTGCGC AGATGGAGGT TATCATGAAA ACGCTGAATA GCAATGGTGG CTTCCCGATC 
ATAAGCCTGT CTGCCTGGTG GTCATCCCCT TGGTGGTCCT ATTTGAAGAT TCTCTTCCCT 
CTCATGTGGT TTCAGACGAA GCTCCTCCAC AAACACTGGG ATAAAGACCC AGTTCAGAAC 
GTGGTGCAGG TCTTGGAGAA ACAGTACCTG GAAGAAAAGC GAAGCGCCCT CGAGGAGCAG 
CGACTCATGT ACGAGCGGGA GCTGGAGCAG CTCCGCCAGC AGCTGTCCCC GGAGAGGCAG 
CCCCAGATCA GCGGCCCCGA CCGCCTGGCC TACAGCAGCC AGACGGCGCA GCAGAAGGTG 
ACCCAGTGGG CCGAGGAGAG GGACGAACTC TTTCGCCAAA GTCTGGCAAA ACTTCGGGAG 
CAGCTGGTGA AAGCCAACAC CCTGGTGAGA GAGGCAAACT TCTTAGCCGA GGAGATGAGC 
AAGCTCACCG ACTACCAGGT GACTCTGCAG ATCCCTGCTG CGAACCTCAG TGCCAACAGG 
AAGAGAGGAG CAATAGTGAG TGAGCCAGCT ATTCAAGTGA GGAGGAAAGG AAAGAGCACC 
CAAGTGTGGA CCATTGAGAA GCTGGAGAAT AAATTAATTG ATATGAGAGA CCTTTATCAA 
GAATGGAAAG AAAAGATTCC TGAGGCAAAG CGACTCTGCG GGAAACGAGG TGACCCTTTC 
TATGAAGCCC AAGAGAATCA CAACCTGATT GGCGTGGCGA ATGTGTTCCT GGAGTGTCTC 
TTCTGTGATG TGAAGCTTCA GTATGCAGTT CCTATCATCA GCCAGCAGGG GGAGGTTGCA 
GGGCGTCTCC ACGTGGAAGT GATGCGAGTG ACAGGCGCTG TCCCGGAGCG CATGGCGGAG 
GACGACTCCT CGGAGAACTC CAGTGAAAGC GGAAGTCTTG AAGTCGTAGA CAGCACCGGG 
GAAATCATTC ACCGAGTCAA GAAGCTGACA TGCCGGGTAA AAATTAAAGA AGCAACTGGG 
CTGCCCTTAA ACCTCTCAAA TTTCGTCTTC TGTCAATACA CGTTCTGGGA CCAGTGTGAG 
CCCACGGTGG CCGCCCCTGT CGTGGATCCT GAGGTGCCTT CCCCACAGTC CAAGGACGCC 
CAGTACACCG TGACCTTCTC CCACTGTCAG GATTACGTGG TCAACGTAAC GGAAGAATTC 
CTGGAGTTCA TTTCAGATGG AGCACTGGCC ATCGAAGTGT GGGGCCACCG GTGTACCGGG 
AACGGCAGCT CCATCTGGGA AGTCGATTCC CTTCATGCTA AGACGAGAAC GCTGCATGAC 
AGGTGGAATG AAGTTACTCG CAGAATAGAG ATGTGGATCT CCATCCTGGA GCTGAACGAG 
CTGGGGGAGT ACGCCGCCGT GGAGCTTCAC CAGGCCAAGG ACGTCAACAC AGGCGGCGTC 
CTTCAGCTCA GACAGGGTCA TTCCCGGAGA GTCCAAGTCA CAGTGAAGCC TGTCCAGCAT 
TCCGGGACAC TGCCACTCAT GGTTGAAGCC ATCTTGTCCA TCTCCATCGG CTGCATCACT 
GCCAGGTCAA CCAAGCTCCA GAGAGGGCTG GACAGTTACC AGAGAGATGA TGAGGATGGT 
GATGATATGG ATAGTTATCA GGAAGAAGAC TTAAACTGCG TGAGAGAGAG GTGGTCGGAT 
GCCCTCATTA AACGGCGAGA ATACCTAGAC GAACAGATTA AAAAAGTCAG CAACAAAAAA 
GAGAAGACGG AGGATGACGT GGAGCGGGAA GCCCGGCTGG TGGAGCAGTG GGTGGGGCTG 
ACGGAGGAGA GGAACGCTGT GCTGGTGCCT GCGCCGGGCA GCGGGATCCC CGGGGCGCCT 
GCCGACTGGA TCCCACCACC TGGAATGGAA ACCCACATAC CGGTTCTCTT CCTTGATTTG 
AACGCGGATG ACCTCAGTGC CAATGAGCAG CTCGTGGGCC CCCACGCATC AGGCGTGAAC 
TCCATCCTCC CCAAGGAGCA CGGCAGCCAG TTTTTCTACC TGCCCATCAT AAAGCACAGT 
GACGAGGAGG TTTCAGCCAC AGCCTCCTGG GACTCCTCGG TGCATGATTC CGTTCACTTG 
AACAGGGTCA CCCCGCAGAA TGAGAGGATC TACCTGATAG TGAAGACCAC AGTCCAGCTC 
AGCCACCCGG CCGCTATGGA GCTGGTCTTA CGAAAACGGA TTGCAGCCAA CATTTACAAC 
AAACAGAGTT TCACCCAGAG TTTGAAGAGG AGAATATCAC TGAAGAATAT ATTTTATTCC 
TGTGGTGTAA CCTATGAAAT AGTATCCAAC ATACCAAAGG CAACAGAGGA GATTGAGGAC 
CGGGAGACGC TGGCTCTCAT GGCTGCAAGG AGTGAGAATG AAGGCACGTC GGATGGGGAG 
ACGTACATCG AGAAGTACAC GCGGGGCGTG CTGCAGGTGG AGAACATTCT GAGCCTGGAA 
CGACTCCGGC AGGCGGTGAC GGTCAAAGAA GCACTTTCCA CCAAAGCTCG GCACCTACGG 
CGAAGCCTCA GCACGCCCAA CGTTCACAAT GTCTCTTCCA GTCGACCAGA CCTTTCTGGC 
TTTGACGATG ATGATAAGGG TTGGCCAGAG AACCAGTTAG ACATGTCTGA CTACAGCCCG 
AGTTACCAGG ATGTATCGTG TTATGGAACT TTGCCTAGGG ATTCACCTCG AAGGAGTAAA 
GAAGGTTGTA CATCAGAGAA TCCTCATGCC TTAACAGTCA GCCCTTTCAA AGCATTCTCT 
CCTCAGCCAC CAAAGTTTTT CAAGCCCCTA ATGCCTGTAA AAGAGGAGCA TAAGAAAAGG 
ATAGCGCTTG AAGCAAGGCC TCTTCTAAGC CAGGAGGACT CTGAGGAGGA GGAAAATGAT 
CTAGAAGGTC TCAGCCGGAA GCTGATAAGT TCACAGCCTT ATGTACCTGT GGAGTTCGCT 
GACTTCAGCG TTTACAATGC CAGCTTGGAG AACAGAGAGT GGTTTTCTTC TAAAGGCGAT 
CTGACGAACT CAAAGGTCCT AGAGAAGGAG GTGTCCCGCA GCCCCACCAC CAGCAGCATC 
ACCAGCGGCT ACTTTTCCCA CAGTGCCTCC AACGCCACCC TGTCCGACAT GGTGGTCCCT 
TCCAGTGACA GCTCAGATCA GTTGGCCCTT CCAACGAAGG ACACAGATTC CAGCGAGCAT 
CCCGGACCGT CCCTTGGGCA GGATTTCAGA CCATCTTCAA ACAAAGAGTT GACAGAACTA 
GAAAGAGGCT TGGGAAAGGA TAAGATGACC GTGGTGCCAC TCAAGGAAAA CAGTGCCTTA 
GCCAAAGGGA GCCCATTGTC CCCCAGTATC CCGGAGAAAA ACTCCAGAAC CCTCTGTAGG 
ACCAGCTCGT GTTCAGAGCA GGATGCCTGC TCCAGCAAAA ACGGCCAGCC GGCCAGGGAA 
TTCTGCCCCA GGGAGGTGAC CGTGGAACAC ACCACAAACA TCCTCGAGGA TCACTCTTTC 
ACAGAGTTTA TGGGTGTGTC AGATGGGAAA GATTTTGATG GTTTGACGGA TTCTTCTGCT 
GGAGAACTTT CAAGTAGGAG GAACCTACCA AATACAGCGG ACAGTAGGAG TTTCTCACAT 
GGGCCACAGG ACCCTGGCCA GCTGTGTTCC TCGGCAGAGA ATGACCAGGG GAGCGGCACT 
GCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_024983945.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839713.1 Bos taurus kinesin family member 13A (KIF13A), transcript variant X4, mRNA.

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CloneID	OBa229712
Clone ID Related Accession (Same CDS sequence)	XM_024983945.1
Accession Version	XM_024983945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGATA CCAAGGTAAA AGTTGCCGTC CGGGTCCGGC CCCTGAACCG ACGAGAACTG 
GAACTCAACA CCAAGTGCGT GGTGGAGATG GAAGGGAATC AAACGGTCCT GCACCCTCCT 
CCTTCTAACA CCAAACAGGG AGAAAGGAAA CCTCCCAAGG TATTTGCATT TGATTATTGC 
TTTTGGTCCA TGGATGAATC CAACACTACA AAGTATGCTG GTCAAGAAGT GGTTTTCAAG 
TGCCTTGGGG AAGGAATTCT TGAAAAAGCC TTTCAGGGGT ATAATGCTTG CATTTTTGCA 
TATGGACAGA CAGGCTCAGG AAAATCCTTC TCCATGATGG GCAATGCTGA GCAGCGAGGC 
CTCATCCCAA GGCTCTGCTG TGCTTTATTT CAAAGGATCT CTCTGGAGCA GAATGAGTCA 
CAGACATTTA AAGTGGAAGT ATCCTATATG GAAATTTATA ATGAGAAAGT CCGGGACCTG 
TTAGACCCCA AAGGGAGTAG ACAGTCTCTT AAAGTTCGAG AGCATAAAGT TTTGGGACCA 
TATGTAGATG GTTTATCCCA ACTGGCTGTC ACTAGTTTTG AGGACATTGA GTCATTGATG 
TCTGAGGGAA ACAAATCTCG GACGGTAGCT GCGACCAACA TGAATGAAGA AAGCAGCCGC 
TCCCATGCAG TGTTCAACAT CGTCATCACG CAGACGCTGT ACGACCTGCA GTCTGGGAAC 
TCAGGGGAGA AGGTGAGCAA GGTGAGCTTG GTGGACCTGG CGGGCAGCGA GAGAGTGTCT 
AAAACTGGAG CTGCGGGAGA GCGCCTGAAA GAAGGCAGCA ACATTAACAA ATCACTTACA 
ACCTTGGGGT TGGTTATCTC ATCGCTGGCT GACCAGGCAG CTGGCAAGGG TAAAAACAAA 
TTTGTGCCTT ATCGCGACTC AGTCCTCACT TGGCTGCTTA AGGACAATCT GGGGGGCAAC 
AGCCAGACCT CCATGATCGC CACCATTAGC CCAGCAGCAG ACAACTACGA AGAGACCCTC 
TCCACGCTGA GATACGCAGA TCGGGCCAAA AGGATCGTTA ACCATGCCGT CGTGAATGAG 
GATCCCAACG CGAAGGTCAT CCGGGAACTG CGGGAAGAGG TGGAGAAACT GAGGGAGCAG 
CTCTCACAGG CTGAGGCTAT GAAGGCCCCT GAACTGAAAG AGAAGCTGGA AGAGTCTGAA 
AAGCTGATAA AAGAGCTAAC AGTGACTTGG GAAGAGAAGC TCAGGAAAAC AGAAGAAATT 
GCACAGGAGA GGCAGCGGCA ACTTGAAAGC ATGGGGATCT CCCTGGAGAC TTCTGGTATC 
AAAGTAGGAG ATGACAAGTG CTACTTGGTC AACCTGAATG CAGACCCTGC TCTCAACGAA 
CTTCTGGTTT ATTATTTAAA GGACCACACC AGGGTAGGTG CCGACACCTC TCAGGACATC 
CAGCTCTTTG GGATAGGAAT TCAGCCTGAG CACTGTGAGA TCGACATTGG CTCTGATGGA 
GAGGTCAGCC TCACTCCAAA AGAAAATGCA CGGTCCTGTG TCAATGGCAC CCTCGTGTGC 
AGCACCACCC AGCTGTGGCA TGGGGACCGA ATCCTGTGGG GAAACAACCA CTTCTTCAGG 
ATTAATTTGC CCAAGAGGAA ACGGCGAGAT TGGTTGAAAG ACTTTGAGAA AGAGACAGGC 
CCGCCGGAGC ACGACCTGGA CGCGGCCAGT GAAGCGTCCT CAGAACCAGA CTATAACTAC 
GAATTTGCGC AGATGGAGGT TATCATGAAA ACGCTGAATA GCAATGACCC AGTTCAGAAC 
GTGGTGCAGG TCTTGGAGAA ACAGTACCTG GAAGAAAAGC GAAGCGCCCT CGAGGAGCAG 
CGACTCATGT ACGAGCGGGA GCTGGAGCAG CTCCGCCAGC AGCTGTCCCC GGAGAGGCAG 
CCCCAGATCA GCGGCCCCGA CCGCCTGGCC TACAGCAGCC AGACGGCGCA GCAGAAGGTG 
ACCCAGTGGG CCGAGGAGAG GGACGAACTC TTTCGCCAAA GTCTGGCAAA ACTTCGGGAG 
CAGCTGGTGA AAGCCAACAC CCTGGTGAGA GAGGCAAACT TCTTAGCCGA GGAGATGAGC 
AAGCTCACCG ACTACCAGGT GACTCTGCAG ATCCCTGCTG CGAACCTCAG TGCCAACAGG 
AAGAGAGGAG CAATAGTGAG TGAGCCAGCT ATTCAAGTGA GGAGGAAAGG AAAGAGCACC 
CAAGTGTGGA CCATTGAGAA GCTGGAGAAT AAATTAATTG ATATGAGAGA CCTTTATCAA 
GAATGGAAAG AAAAGATTCC TGAGGCAAAG CGACTCTGCG GGAAACGAGG TGACCCTTTC 
TATGAAGCCC AAGAGAATCA CAACCTGATT GGCGTGGCGA ATGTGTTCCT GGAGTGTCTC 
TTCTGTGATG TGAAGCTTCA GTATGCAGTT CCTATCATCA GCCAGCAGGG GGAGGTTGCA 
GGGCGTCTCC ACGTGGAAGT GATGCGAGTG ACAGGCGCTG TCCCGGAGCG CATGGCGGAG 
GACGACTCCT CGGAGAACTC CAGTGAAAGC GGAAGTCTTG AAGTCGTAGA CAGCACCGGG 
GAAATCATTC ACCGAGTCAA GAAGCTGACA TGCCGGGTAA AAATTAAAGA AGCAACTGGG 
CTGCCCTTAA ACCTCTCAAA TTTCGTCTTC TGTCAATACA CGTTCTGGGA CCAGTGTGAG 
CCCACGGTGG CCGCCCCTGT CGTGGATCCT GAGGTGCCTT CCCCACAGTC CAAGGACGCC 
CAGTACACCG TGACCTTCTC CCACTGTCAG GATTACGTGG TCAACGTAAC GGAAGAATTC 
CTGGAGTTCA TTTCAGATGG AGCACTGGCC ATCGAAGTGT GGGGCCACCG GTGTACCGGG 
AACGGCAGCT CCATCTGGGA AGTCGATTCC CTTCATGCTA AGACGAGAAC GCTGCATGAC 
AGGTGGAATG AAGTTACTCG CAGAATAGAG ATGTGGATCT CCATCCTGGA GCTGAACGAG 
CTGGGGGAGT ACGCCGCCGT GGAGCTTCAC CAGGCCAAGG ACGTCAACAC AGGCGGCGTC 
CTTCAGCTCA GACAGGGTCA TTCCCGGAGA GTCCAAGTCA CAGTGAAGCC TGTCCAGCAT 
TCCGGGACAC TGCCACTCAT GGTTGAAGCC ATCTTGTCCA TCTCCATCGG CTGCATCACT 
GCCAGGTCAA CCAAGCTCCA GAGAGGGCTG GACAGTTACC AGAGAGATGA TGAGGATGGT 
GATGATATGG ATAGTTATCA GGAAGAAGAC TTAAACTGCG TGAGAGAGAG GTGGTCGGAT 
GCCCTCATTA AACGGCGAGA ATACCTAGAC GAACAGATTA AAAAAGTCAG CAACAAAAAA 
GAGAAGACGG AGGATGACGT GGAGCGGGAA GCCCGGCTGG TGGAGCAGTG GGTGGGGCTG 
ACGGAGGAGA GGAACGCTGT GCTGGTGCCT GCGCCGGGCA GCGGGATCCC CGGGGCGCCT 
GCCGACTGGA TCCCACCACC TGGAATGGAA ACCCACATAC CGGTTCTCTT CCTTGATTTG 
AACGCGGATG ACCTCAGTGC CAATGAGCAG CTCGTGGGCC CCCACGCATC AGGCGTGAAC 
TCCATCCTCC CCAAGGAGCA CGGCAGCCAG TTTTTCTACC TGCCCATCAT AAAGCACAGT 
GACGAGGAGG TTTCAGCCAC AGCCTCCTGG GACTCCTCGG TGCATGATTC CGTTCACTTG 
AACAGGGTCA CCCCGCAGAA TGAGAGGATC TACCTGATAG TGAAGACCAC AGTCCAGCTC 
AGCCACCCGG CCGCTATGGA GCTGGTCTTA CGAAAACGGA TTGCAGCCAA CATTTACAAC 
AAACAGAGTT TCACCCAGAG TTTGAAGAGG AGAATATCAC TGAAGAATAT ATTTTATTCC 
TGTGGTGTAA CCTATGAAAT AGTATCCAAC ATACCAAAGG CAACAGAGGA GATTGAGGAC 
CGGGAGACGC TGGCTCTCAT GGCTGCAAGG AGTGAGAATG AAGGCACGTC GGATGGGGAG 
ACGTACATCG AGAAGTACAC GCGGGGCGTG CTGCAGGTGG AGAACATTCT GAGCCTGGAA 
CGACTCCGGC AGGCGGTGAC GGTCAAAGAA GCACTTTCCA CCAAAGCTCG GCACCTACGG 
CGAAGCCTCA GCACGCCCAA CGTTCACAAT GTCTCTTCCA GTCGACCAGA CCTTTCTGGC 
TTTGACGATG ATGATAAGGG TTGGCCAGAG AACCAGTTAG ACATGTCTGA CTACAGCCCG 
AGTTACCAGG ATGTATCGTG TTATGGAACT TTGCCTAGGG ATTCACCTCG AAGGAGTAAA 
GAAGGTTGTA CATCAGAGAA TCCTCATGCC TTAACAGTCA GCCCTTTCAA AGCATTCTCT 
CCTCAGCCAC CAAAGTTTTT CAAGCCCCTA ATGCCTGTAA AAGAGGAGCA TAAGAAAAGG 
ATAGCGCTTG AAGCAAGGCC TCTTCTAAGC CAGGAGAGCA TGTCTCCATC TCAGGCACAT 
AATCCTGGCT GCATTGTGCC CTCGGGAAGC AATGGCAGCA GCATGCCAGT AGAACTCAAT 
AGCAAACGTG AGAAGAAGAT TGACTCTGAG GAGGAGGAAA ATGATCTAGA AGGTCTCAGC 
CGGAAGCTGA TAAGTTCACA GCCTTATGTA CCTGTGGAGT TCGCTGACTT CAGCGTTTAC 
AATGCCAGCT TGGAGAACAG AGAGTGGTTT TCTTCTAAAG GCGATCTGAC GAACTCAAAG 
GTCCTAGAGA AGGAGGTGTC CCGCAGCCCC ACCACCAGCA GCATCACCAG CGGCTACTTT 
TCCCACAGTG CCTCCAACGC CACCCTGTCC GACATGGTGG TCCCTTCCAG TGACAGCTCA 
GATCAGTTGG CCCTTCCAAC GAAGGACACA GATTCCAGCG AGCATCCCGG ACCGTCCCTT 
GGGCAGGATT TCAGACCATC TTCAAACAAA GAGTTGACAG AACTAGAAAG AGGCTTGGGA 
AAGGATAAGA TGACCGTGGT GCCACTCAAG GAAAACAGTG CCTTAGCCAA AGGGAGCCCA 
TTGTCCCCCA GTATCCCGGA GAAAAACTCC AGAACCCTCT GTAGGACCAG CTCGTGTTCA 
GAGCAGGATG CCTGCTCCAG CAAAAACGGC CAGCCGGCCA GGGAATTCTG CCCCAGGGAG 
GTGACCGTGG AACACACCAC AAACATCCTC GAGGATCACT CTTTCACAGA GTTTATGGGT 
GTGTCAGATG GGAAAGATTT TGATGGTTTG ACGGATTCTT CTGCTGGAGA ACTTTCAAGT 
AGGAGGAACC TACCAAATAC AGCGGACAGT AGGAGTTTCT CACATGGGCC ACAGGACCCT 
GGCCAGCTGT GTTCCTCGGC AGAGAATGAC CAGGGGAGCG GCACTGCTCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839713.1
CDS	123..5516
Translation	MSDTKVKVAVRVRPLNRRELELNTKCVVEMEGNQTVLHPPPSNTKQGERKPPKVFAFDYCFWSMDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQESMSPSQAHNPGCIVPSGSNGSSMPVELNSKREKKIDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_024983945.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 13A (KIF13A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983945.1

ORF Insert Sequence:

ATGTCGGATA CCAAGGTAAA AGTTGCCGTC CGGGTCCGGC CCCTGAACCG ACGAGAACTG 
GAACTCAACA CCAAGTGCGT GGTGGAGATG GAAGGGAATC AAACGGTCCT GCACCCTCCT 
CCTTCTAACA CCAAACAGGG AGAAAGGAAA CCTCCCAAGG TATTTGCATT TGATTATTGC 
TTTTGGTCCA TGGATGAATC CAACACTACA AAGTATGCTG GTCAAGAAGT GGTTTTCAAG 
TGCCTTGGGG AAGGAATTCT TGAAAAAGCC TTTCAGGGGT ATAATGCTTG CATTTTTGCA 
TATGGACAGA CAGGCTCAGG AAAATCCTTC TCCATGATGG GCAATGCTGA GCAGCGAGGC 
CTCATCCCAA GGCTCTGCTG TGCTTTATTT CAAAGGATCT CTCTGGAGCA GAATGAGTCA 
CAGACATTTA AAGTGGAAGT ATCCTATATG GAAATTTATA ATGAGAAAGT CCGGGACCTG 
TTAGACCCCA AAGGGAGTAG ACAGTCTCTT AAAGTTCGAG AGCATAAAGT TTTGGGACCA 
TATGTAGATG GTTTATCCCA ACTGGCTGTC ACTAGTTTTG AGGACATTGA GTCATTGATG 
TCTGAGGGAA ACAAATCTCG GACGGTAGCT GCGACCAACA TGAATGAAGA AAGCAGCCGC 
TCCCATGCAG TGTTCAACAT CGTCATCACG CAGACGCTGT ACGACCTGCA GTCTGGGAAC 
TCAGGGGAGA AGGTGAGCAA GGTGAGCTTG GTGGACCTGG CGGGCAGCGA GAGAGTGTCT 
AAAACTGGAG CTGCGGGAGA GCGCCTGAAA GAAGGCAGCA ACATTAACAA ATCACTTACA 
ACCTTGGGGT TGGTTATCTC ATCGCTGGCT GACCAGGCAG CTGGCAAGGG TAAAAACAAA 
TTTGTGCCTT ATCGCGACTC AGTCCTCACT TGGCTGCTTA AGGACAATCT GGGGGGCAAC 
AGCCAGACCT CCATGATCGC CACCATTAGC CCAGCAGCAG ACAACTACGA AGAGACCCTC 
TCCACGCTGA GATACGCAGA TCGGGCCAAA AGGATCGTTA ACCATGCCGT CGTGAATGAG 
GATCCCAACG CGAAGGTCAT CCGGGAACTG CGGGAAGAGG TGGAGAAACT GAGGGAGCAG 
CTCTCACAGG CTGAGGCTAT GAAGGCCCCT GAACTGAAAG AGAAGCTGGA AGAGTCTGAA 
AAGCTGATAA AAGAGCTAAC AGTGACTTGG GAAGAGAAGC TCAGGAAAAC AGAAGAAATT 
GCACAGGAGA GGCAGCGGCA ACTTGAAAGC ATGGGGATCT CCCTGGAGAC TTCTGGTATC 
AAAGTAGGAG ATGACAAGTG CTACTTGGTC AACCTGAATG CAGACCCTGC TCTCAACGAA 
CTTCTGGTTT ATTATTTAAA GGACCACACC AGGGTAGGTG CCGACACCTC TCAGGACATC 
CAGCTCTTTG GGATAGGAAT TCAGCCTGAG CACTGTGAGA TCGACATTGG CTCTGATGGA 
GAGGTCAGCC TCACTCCAAA AGAAAATGCA CGGTCCTGTG TCAATGGCAC CCTCGTGTGC 
AGCACCACCC AGCTGTGGCA TGGGGACCGA ATCCTGTGGG GAAACAACCA CTTCTTCAGG 
ATTAATTTGC CCAAGAGGAA ACGGCGAGAT TGGTTGAAAG ACTTTGAGAA AGAGACAGGC 
CCGCCGGAGC ACGACCTGGA CGCGGCCAGT GAAGCGTCCT CAGAACCAGA CTATAACTAC 
GAATTTGCGC AGATGGAGGT TATCATGAAA ACGCTGAATA GCAATGACCC AGTTCAGAAC 
GTGGTGCAGG TCTTGGAGAA ACAGTACCTG GAAGAAAAGC GAAGCGCCCT CGAGGAGCAG 
CGACTCATGT ACGAGCGGGA GCTGGAGCAG CTCCGCCAGC AGCTGTCCCC GGAGAGGCAG 
CCCCAGATCA GCGGCCCCGA CCGCCTGGCC TACAGCAGCC AGACGGCGCA GCAGAAGGTG 
ACCCAGTGGG CCGAGGAGAG GGACGAACTC TTTCGCCAAA GTCTGGCAAA ACTTCGGGAG 
CAGCTGGTGA AAGCCAACAC CCTGGTGAGA GAGGCAAACT TCTTAGCCGA GGAGATGAGC 
AAGCTCACCG ACTACCAGGT GACTCTGCAG ATCCCTGCTG CGAACCTCAG TGCCAACAGG 
AAGAGAGGAG CAATAGTGAG TGAGCCAGCT ATTCAAGTGA GGAGGAAAGG AAAGAGCACC 
CAAGTGTGGA CCATTGAGAA GCTGGAGAAT AAATTAATTG ATATGAGAGA CCTTTATCAA 
GAATGGAAAG AAAAGATTCC TGAGGCAAAG CGACTCTGCG GGAAACGAGG TGACCCTTTC 
TATGAAGCCC AAGAGAATCA CAACCTGATT GGCGTGGCGA ATGTGTTCCT GGAGTGTCTC 
TTCTGTGATG TGAAGCTTCA GTATGCAGTT CCTATCATCA GCCAGCAGGG GGAGGTTGCA 
GGGCGTCTCC ACGTGGAAGT GATGCGAGTG ACAGGCGCTG TCCCGGAGCG CATGGCGGAG 
GACGACTCCT CGGAGAACTC CAGTGAAAGC GGAAGTCTTG AAGTCGTAGA CAGCACCGGG 
GAAATCATTC ACCGAGTCAA GAAGCTGACA TGCCGGGTAA AAATTAAAGA AGCAACTGGG 
CTGCCCTTAA ACCTCTCAAA TTTCGTCTTC TGTCAATACA CGTTCTGGGA CCAGTGTGAG 
CCCACGGTGG CCGCCCCTGT CGTGGATCCT GAGGTGCCTT CCCCACAGTC CAAGGACGCC 
CAGTACACCG TGACCTTCTC CCACTGTCAG GATTACGTGG TCAACGTAAC GGAAGAATTC 
CTGGAGTTCA TTTCAGATGG AGCACTGGCC ATCGAAGTGT GGGGCCACCG GTGTACCGGG 
AACGGCAGCT CCATCTGGGA AGTCGATTCC CTTCATGCTA AGACGAGAAC GCTGCATGAC 
AGGTGGAATG AAGTTACTCG CAGAATAGAG ATGTGGATCT CCATCCTGGA GCTGAACGAG 
CTGGGGGAGT ACGCCGCCGT GGAGCTTCAC CAGGCCAAGG ACGTCAACAC AGGCGGCGTC 
CTTCAGCTCA GACAGGGTCA TTCCCGGAGA GTCCAAGTCA CAGTGAAGCC TGTCCAGCAT 
TCCGGGACAC TGCCACTCAT GGTTGAAGCC ATCTTGTCCA TCTCCATCGG CTGCATCACT 
GCCAGGTCAA CCAAGCTCCA GAGAGGGCTG GACAGTTACC AGAGAGATGA TGAGGATGGT 
GATGATATGG ATAGTTATCA GGAAGAAGAC TTAAACTGCG TGAGAGAGAG GTGGTCGGAT 
GCCCTCATTA AACGGCGAGA ATACCTAGAC GAACAGATTA AAAAAGTCAG CAACAAAAAA 
GAGAAGACGG AGGATGACGT GGAGCGGGAA GCCCGGCTGG TGGAGCAGTG GGTGGGGCTG 
ACGGAGGAGA GGAACGCTGT GCTGGTGCCT GCGCCGGGCA GCGGGATCCC CGGGGCGCCT 
GCCGACTGGA TCCCACCACC TGGAATGGAA ACCCACATAC CGGTTCTCTT CCTTGATTTG 
AACGCGGATG ACCTCAGTGC CAATGAGCAG CTCGTGGGCC CCCACGCATC AGGCGTGAAC 
TCCATCCTCC CCAAGGAGCA CGGCAGCCAG TTTTTCTACC TGCCCATCAT AAAGCACAGT 
GACGAGGAGG TTTCAGCCAC AGCCTCCTGG GACTCCTCGG TGCATGATTC CGTTCACTTG 
AACAGGGTCA CCCCGCAGAA TGAGAGGATC TACCTGATAG TGAAGACCAC AGTCCAGCTC 
AGCCACCCGG CCGCTATGGA GCTGGTCTTA CGAAAACGGA TTGCAGCCAA CATTTACAAC 
AAACAGAGTT TCACCCAGAG TTTGAAGAGG AGAATATCAC TGAAGAATAT ATTTTATTCC 
TGTGGTGTAA CCTATGAAAT AGTATCCAAC ATACCAAAGG CAACAGAGGA GATTGAGGAC 
CGGGAGACGC TGGCTCTCAT GGCTGCAAGG AGTGAGAATG AAGGCACGTC GGATGGGGAG 
ACGTACATCG AGAAGTACAC GCGGGGCGTG CTGCAGGTGG AGAACATTCT GAGCCTGGAA 
CGACTCCGGC AGGCGGTGAC GGTCAAAGAA GCACTTTCCA CCAAAGCTCG GCACCTACGG 
CGAAGCCTCA GCACGCCCAA CGTTCACAAT GTCTCTTCCA GTCGACCAGA CCTTTCTGGC 
TTTGACGATG ATGATAAGGG TTGGCCAGAG AACCAGTTAG ACATGTCTGA CTACAGCCCG 
AGTTACCAGG ATGTATCGTG TTATGGAACT TTGCCTAGGG ATTCACCTCG AAGGAGTAAA 
GAAGGTTGTA CATCAGAGAA TCCTCATGCC TTAACAGTCA GCCCTTTCAA AGCATTCTCT 
CCTCAGCCAC CAAAGTTTTT CAAGCCCCTA ATGCCTGTAA AAGAGGAGCA TAAGAAAAGG 
ATAGCGCTTG AAGCAAGGCC TCTTCTAAGC CAGGAGAGCA TGTCTCCATC TCAGGCACAT 
AATCCTGGCT GCATTGTGCC CTCGGGAAGC AATGGCAGCA GCATGCCAGT AGAACTCAAT 
AGCAAACGTG AGAAGAAGAT TGACTCTGAG GAGGAGGAAA ATGATCTAGA AGGTCTCAGC 
CGGAAGCTGA TAAGTTCACA GCCTTATGTA CCTGTGGAGT TCGCTGACTT CAGCGTTTAC 
AATGCCAGCT TGGAGAACAG AGAGTGGTTT TCTTCTAAAG GCGATCTGAC GAACTCAAAG 
GTCCTAGAGA AGGAGGTGTC CCGCAGCCCC ACCACCAGCA GCATCACCAG CGGCTACTTT 
TCCCACAGTG CCTCCAACGC CACCCTGTCC GACATGGTGG TCCCTTCCAG TGACAGCTCA 
GATCAGTTGG CCCTTCCAAC GAAGGACACA GATTCCAGCG AGCATCCCGG ACCGTCCCTT 
GGGCAGGATT TCAGACCATC TTCAAACAAA GAGTTGACAG AACTAGAAAG AGGCTTGGGA 
AAGGATAAGA TGACCGTGGT GCCACTCAAG GAAAACAGTG CCTTAGCCAA AGGGAGCCCA 
TTGTCCCCCA GTATCCCGGA GAAAAACTCC AGAACCCTCT GTAGGACCAG CTCGTGTTCA 
GAGCAGGATG CCTGCTCCAG CAAAAACGGC CAGCCGGCCA GGGAATTCTG CCCCAGGGAG 
GTGACCGTGG AACACACCAC AAACATCCTC GAGGATCACT CTTTCACAGA GTTTATGGGT 
GTGTCAGATG GGAAAGATTT TGATGGTTTG ACGGATTCTT CTGCTGGAGA ACTTTCAAGT 
AGGAGGAACC TACCAAATAC AGCGGACAGT AGGAGTTTCT CACATGGGCC ACAGGACCCT 
GGCCAGCTGT GTTCCTCGGC AGAGAATGAC CAGGGGAGCG GCACTGCTCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_024983946.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839714.1 Bos taurus kinesin family member 13A (KIF13A), transcript variant X5, mRNA.

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CloneID	OBa229713
Clone ID Related Accession (Same CDS sequence)	XM_024983946.1
Accession Version	XM_024983946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGATA CCAAGGTAAA AGTTGCCGTC CGGGTCCGGC CCCTGAACCG ACGAGAACTG 
GAACTCAACA CCAAGTGCGT GGTGGAGATG GAAGGGAATC AAACGGTCCT GCACCCTCCT 
CCTTCTAACA CCAAACAGGG AGAAAGGAAA CCTCCCAAGG TATTTGCATT TGATTATTGC 
TTTTGGTCCA TGGATGAATC CAACACTACA AAGTATGCTG GTCAAGAAGT GGTTTTCAAG 
TGCCTTGGGG AAGGAATTCT TGAAAAAGCC TTTCAGGGGT ATAATGCTTG CATTTTTGCA 
TATGGACAGA CAGGCTCAGG AAAATCCTTC TCCATGATGG GCAATGCTGA GCAGCGAGGC 
CTCATCCCAA GGCTCTGCTG TGCTTTATTT CAAAGGATCT CTCTGGAGCA GAATGAGTCA 
CAGACATTTA AAGTGGAAGT ATCCTATATG GAAATTTATA ATGAGAAAGT CCGGGACCTG 
TTAGACCCCA AAGGGAGTAG ACAGTCTCTT AAAGTTCGAG AGCATAAAGT TTTGGGACCA 
TATGTAGATG GTTTATCCCA ACTGGCTGTC ACTAGTTTTG AGGACATTGA GTCATTGATG 
TCTGAGGGAA ACAAATCTCG GACGGTAGCT GCGACCAACA TGAATGAAGA AAGCAGCCGC 
TCCCATGCAG TGTTCAACAT CGTCATCACG CAGACGCTGT ACGACCTGCA GTCTGGGAAC 
TCAGGGGAGA AGGTGAGCAA GGTGAGCTTG GTGGACCTGG CGGGCAGCGA GAGAGTGTCT 
AAAACTGGAG CTGCGGGAGA GCGCCTGAAA GAAGGCAGCA ACATTAACAA ATCACTTACA 
ACCTTGGGGT TGGTTATCTC ATCGCTGGCT GACCAGGCAG CTGGCAAGGG TAAAAACAAA 
TTTGTGCCTT ATCGCGACTC AGTCCTCACT TGGCTGCTTA AGGACAATCT GGGGGGCAAC 
AGCCAGACCT CCATGATCGC CACCATTAGC CCAGCAGCAG ACAACTACGA AGAGACCCTC 
TCCACGCTGA GATACGCAGA TCGGGCCAAA AGGATCGTTA ACCATGCCGT CGTGAATGAG 
GATCCCAACG CGAAGGTCAT CCGGGAACTG CGGGAAGAGG TGGAGAAACT GAGGGAGCAG 
CTCTCACAGG CTGAGGCTAT GAAGGCCCCT GAACTGAAAG AGAAGCTGGA AGAGTCTGAA 
AAGCTGATAA AAGAGCTAAC AGTGACTTGG GAAGAGAAGC TCAGGAAAAC AGAAGAAATT 
GCACAGGAGA GGCAGCGGCA ACTTGAAAGC ATGGGGATCT CCCTGGAGAC TTCTGGTATC 
AAAGTAGGAG ATGACAAGTG CTACTTGGTC AACCTGAATG CAGACCCTGC TCTCAACGAA 
CTTCTGGTTT ATTATTTAAA GGACCACACC AGGGTAGGTG CCGACACCTC TCAGGACATC 
CAGCTCTTTG GGATAGGAAT TCAGCCTGAG CACTGTGAGA TCGACATTGG CTCTGATGGA 
GAGGTCAGCC TCACTCCAAA AGAAAATGCA CGGTCCTGTG TCAATGGCAC CCTCGTGTGC 
AGCACCACCC AGCTGTGGCA TGGGGACCGA ATCCTGTGGG GAAACAACCA CTTCTTCAGG 
ATTAATTTGC CCAAGAGGAA ACGGCGAGAT TGGTTGAAAG ACTTTGAGAA AGAGACAGGC 
CCGCCGGAGC ACGACCTGGA CGCGGCCAGT GAAGCGTCCT CAGAACCAGA CTATAACTAC 
GAATTTGCGC AGATGGAGGT TATCATGAAA ACGCTGAATA GCAATGACCC AGTTCAGAAC 
GTGGTGCAGG TCTTGGAGAA ACAGTACCTG GAAGAAAAGC GAAGCGCCCT CGAGGAGCAG 
CGACTCATGT ACGAGCGGGA GCTGGAGCAG CTCCGCCAGC AGCTGTCCCC GGAGAGGCAG 
CCCCAGATCA GCGGCCCCGA CCGCCTGGCC TACAGCAGCC AGACGGCGCA GCAGAAGGTG 
ACCCAGTGGG CCGAGGAGAG GGACGAACTC TTTCGCCAAA GTCTGGCAAA ACTTCGGGAG 
CAGCTGGTGA AAGCCAACAC CCTGGTGAGA GAGGCAAACT TCTTAGCCGA GGAGATGAGC 
AAGCTCACCG ACTACCAGGT GACTCTGCAG ATCCCTGCTG CGAACCTCAG TGCCAACAGG 
AAGAGAGGAG CAATAGTGAG TGAGCCAGCT ATTCAAGTGA GGAGGAAAGG AAAGAGCACC 
CAAGTGTGGA CCATTGAGAA GCTGGAGAAT AAATTAATTG ATATGAGAGA CCTTTATCAA 
GAATGGAAAG AAAAGATTCC TGAGGCAAAG CGACTCTGCG GGAAACGAGG TGACCCTTTC 
TATGAAGCCC AAGAGAATCA CAACCTGATT GGCGTGGCGA ATGTGTTCCT GGAGTGTCTC 
TTCTGTGATG TGAAGCTTCA GTATGCAGTT CCTATCATCA GCCAGCAGGG GGAGGTTGCA 
GGGCGTCTCC ACGTGGAAGT GATGCGAGTG ACAGGCGCTG TCCCGGAGCG CATGGCGGAG 
GACGACTCCT CGGAGAACTC CAGTGAAAGC GGAAGTCTTG AAGTCGTAGA CAGCACCGGG 
GAAATCATTC ACCGAGTCAA GAAGCTGACA TGCCGGGTAA AAATTAAAGA AGCAACTGGG 
CTGCCCTTAA ACCTCTCAAA TTTCGTCTTC TGTCAATACA CGTTCTGGGA CCAGTGTGAG 
CCCACGGTGG CCGCCCCTGT CGTGGATCCT GAGGTGCCTT CCCCACAGTC CAAGGACGCC 
CAGTACACCG TGACCTTCTC CCACTGTCAG GATTACGTGG TCAACGTAAC GGAAGAATTC 
CTGGAGTTCA TTTCAGATGG AGCACTGGCC ATCGAAGTGT GGGGCCACCG GTGTACCGGG 
AACGGCAGCT CCATCTGGGA AGTCGATTCC CTTCATGCTA AGACGAGAAC GCTGCATGAC 
AGGTGGAATG AAGTTACTCG CAGAATAGAG ATGTGGATCT CCATCCTGGA GCTGAACGAG 
CTGGGGGAGT ACGCCGCCGT GGAGCTTCAC CAGGCCAAGG ACGTCAACAC AGGCGGCGTC 
CTTCAGCTCA GACAGGGTCA TTCCCGGAGA GTCCAAGTCA CAGTGAAGCC TGTCCAGCAT 
TCCGGGACAC TGCCACTCAT GGTTGAAGCC ATCTTGTCCA TCTCCATCGG CTGCATCACT 
GCCAGGTCAA CCAAGCTCCA GAGAGGGCTG GACAGTTACC AGAGAGATGA TGAGGATGGT 
GATGATATGG ATAGTTATCA GGAAGAAGAC TTAAACTGCG TGAGAGAGAG GTGGTCGGAT 
GCCCTCATTA AACGGCGAGA ATACCTAGAC GAACAGATTA AAAAAGTCAG CAACAAAAAA 
GAGAAGACGG AGGATGACGT GGAGCGGGAA GCCCGGCTGG TGGAGCAGTG GGTGGGGCTG 
ACGGAGGAGA GGAACGCTGT GCTGGTGCCT GCGCCGGGCA GCGGGATCCC CGGGGCGCCT 
GCCGACTGGA TCCCACCACC TGGAATGGAA ACCCACATAC CGGTTCTCTT CCTTGATTTG 
AACGCGGATG ACCTCAGTGC CAATGAGCAG CTCGTGGGCC CCCACGCATC AGGCGTGAAC 
TCCATCCTCC CCAAGGAGCA CGGCAGCCAG TTTTTCTACC TGCCCATCAT AAAGCACAGT 
GACGAGGAGG TTTCAGCCAC AGCCTCCTGG GACTCCTCGG TGCATGATTC CGTTCACTTG 
AACAGGGTCA CCCCGCAGAA TGAGAGGATC TACCTGATAG TGAAGACCAC AGTCCAGCTC 
AGCCACCCGG CCGCTATGGA GCTGGTCTTA CGAAAACGGA TTGCAGCCAA CATTTACAAC 
AAACAGAGTT TCACCCAGAG TTTGAAGAGG AGAATATCAC TGAAGAATAT ATTTTATTCC 
TGTGGTGTAA CCTATGAAAT AGTATCCAAC ATACCAAAGG CAACAGAGGA GATTGAGGAC 
CGGGAGACGC TGGCTCTCAT GGCTGCAAGG AGTGAGAATG AAGGCACGTC GGATGGGGAG 
ACGTACATCG AGAAGTACAC GCGGGGCGTG CTGCAGGTGG AGAACATTCT GAGCCTGGAA 
CGACTCCGGC AGGCGGTGAC GGTCAAAGAA GCACTTTCCA CCAAAGCTCG GCACCTACGG 
CGAAGCCTCA GCACGCCCAA CGTTCACAAT GTCTCTTCCA GTCGACCAGA CCTTTCTGGC 
TTTGACGATG ATGATAAGGG TTGGCCAGAG AACCAGTTAG ACATGTCTGA CTACAGCCCG 
AGTTACCAGG ATGTATCGTG TTATGGAACT TTGCCTAGGG ATTCACCTCG AAGGAGTAAA 
GAAGGTTGTA CATCAGAGAA TCCTCATGCC TTAACAGTCA GCCCTTTCAA AGCATTCTCT 
CCTCAGCCAC CAAAGTTTTT CAAGCCCCTA ATGCCTGTAA AAGAGGAGCA TAAGAAAAGG 
ATAGCGCTTG AAGCAAGGCC TCTTCTAAGC CAGGAGGACT CTGAGGAGGA GGAAAATGAT 
CTAGAAGGTC TCAGCCGGAA GCTGATAAGT TCACAGCCTT ATGTACCTGT GGAGTTCGCT 
GACTTCAGCG TTTACAATGC CAGCTTGGAG AACAGAGAGT GGTTTTCTTC TAAAGGCGAT 
CTGACGAACT CAAAGGTCCT AGAGAAGGAG GTGTCCCGCA GCCCCACCAC CAGCAGCATC 
ACCAGCGGCT ACTTTTCCCA CAGTGCCTCC AACGCCACCC TGTCCGACAT GGTGGTCCCT 
TCCAGTGACA GCTCAGATCA GTTGGCCCTT CCAACGAAGG ACACAGATTC CAGCGAGCAT 
CCCGGACCGT CCCTTGGGCA GGATTTCAGA CCATCTTCAA ACAAAGAGTT GACAGAACTA 
GAAAGAGGCT TGGGAAAGGA TAAGATGACC GTGGTGCCAC TCAAGGAAAA CAGTGCCTTA 
GCCAAAGGGA GCCCATTGTC CCCCAGTATC CCGGAGAAAA ACTCCAGAAC CCTCTGTAGG 
ACCAGCTCGT GTTCAGAGCA GGATGCCTGC TCCAGCAAAA ACGGCCAGCC GGCCAGGGAA 
TTCTGCCCCA GGGAGGTGAC CGTGGAACAC ACCACAAACA TCCTCGAGGA TCACTCTTTC 
ACAGAGTTTA TGGGTGTGTC AGATGGGAAA GATTTTGATG GTTTGACGGA TTCTTCTGCT 
GGAGAACTTT CAAGTAGGAG GAACCTACCA AATACAGCGG ACAGTAGGAG TTTCTCACAT 
GGGCCACAGG ACCCTGGCCA GCTGTGTTCC TCGGCAGAGA ATGACCAGGG GAGCGGCACT 
GCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839714.1
CDS	1..5289
Translation	MSDTKVKVAVRVRPLNRRELELNTKCVVEMEGNQTVLHPPPSNTKQGERKPPKVFAFDYCFWSMDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQEDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_024983946.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 13A (KIF13A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983946.1

ORF Insert Sequence:

ATGTCGGATA CCAAGGTAAA AGTTGCCGTC CGGGTCCGGC CCCTGAACCG ACGAGAACTG 
GAACTCAACA CCAAGTGCGT GGTGGAGATG GAAGGGAATC AAACGGTCCT GCACCCTCCT 
CCTTCTAACA CCAAACAGGG AGAAAGGAAA CCTCCCAAGG TATTTGCATT TGATTATTGC 
TTTTGGTCCA TGGATGAATC CAACACTACA AAGTATGCTG GTCAAGAAGT GGTTTTCAAG 
TGCCTTGGGG AAGGAATTCT TGAAAAAGCC TTTCAGGGGT ATAATGCTTG CATTTTTGCA 
TATGGACAGA CAGGCTCAGG AAAATCCTTC TCCATGATGG GCAATGCTGA GCAGCGAGGC 
CTCATCCCAA GGCTCTGCTG TGCTTTATTT CAAAGGATCT CTCTGGAGCA GAATGAGTCA 
CAGACATTTA AAGTGGAAGT ATCCTATATG GAAATTTATA ATGAGAAAGT CCGGGACCTG 
TTAGACCCCA AAGGGAGTAG ACAGTCTCTT AAAGTTCGAG AGCATAAAGT TTTGGGACCA 
TATGTAGATG GTTTATCCCA ACTGGCTGTC ACTAGTTTTG AGGACATTGA GTCATTGATG 
TCTGAGGGAA ACAAATCTCG GACGGTAGCT GCGACCAACA TGAATGAAGA AAGCAGCCGC 
TCCCATGCAG TGTTCAACAT CGTCATCACG CAGACGCTGT ACGACCTGCA GTCTGGGAAC 
TCAGGGGAGA AGGTGAGCAA GGTGAGCTTG GTGGACCTGG CGGGCAGCGA GAGAGTGTCT 
AAAACTGGAG CTGCGGGAGA GCGCCTGAAA GAAGGCAGCA ACATTAACAA ATCACTTACA 
ACCTTGGGGT TGGTTATCTC ATCGCTGGCT GACCAGGCAG CTGGCAAGGG TAAAAACAAA 
TTTGTGCCTT ATCGCGACTC AGTCCTCACT TGGCTGCTTA AGGACAATCT GGGGGGCAAC 
AGCCAGACCT CCATGATCGC CACCATTAGC CCAGCAGCAG ACAACTACGA AGAGACCCTC 
TCCACGCTGA GATACGCAGA TCGGGCCAAA AGGATCGTTA ACCATGCCGT CGTGAATGAG 
GATCCCAACG CGAAGGTCAT CCGGGAACTG CGGGAAGAGG TGGAGAAACT GAGGGAGCAG 
CTCTCACAGG CTGAGGCTAT GAAGGCCCCT GAACTGAAAG AGAAGCTGGA AGAGTCTGAA 
AAGCTGATAA AAGAGCTAAC AGTGACTTGG GAAGAGAAGC TCAGGAAAAC AGAAGAAATT 
GCACAGGAGA GGCAGCGGCA ACTTGAAAGC ATGGGGATCT CCCTGGAGAC TTCTGGTATC 
AAAGTAGGAG ATGACAAGTG CTACTTGGTC AACCTGAATG CAGACCCTGC TCTCAACGAA 
CTTCTGGTTT ATTATTTAAA GGACCACACC AGGGTAGGTG CCGACACCTC TCAGGACATC 
CAGCTCTTTG GGATAGGAAT TCAGCCTGAG CACTGTGAGA TCGACATTGG CTCTGATGGA 
GAGGTCAGCC TCACTCCAAA AGAAAATGCA CGGTCCTGTG TCAATGGCAC CCTCGTGTGC 
AGCACCACCC AGCTGTGGCA TGGGGACCGA ATCCTGTGGG GAAACAACCA CTTCTTCAGG 
ATTAATTTGC CCAAGAGGAA ACGGCGAGAT TGGTTGAAAG ACTTTGAGAA AGAGACAGGC 
CCGCCGGAGC ACGACCTGGA CGCGGCCAGT GAAGCGTCCT CAGAACCAGA CTATAACTAC 
GAATTTGCGC AGATGGAGGT TATCATGAAA ACGCTGAATA GCAATGACCC AGTTCAGAAC 
GTGGTGCAGG TCTTGGAGAA ACAGTACCTG GAAGAAAAGC GAAGCGCCCT CGAGGAGCAG 
CGACTCATGT ACGAGCGGGA GCTGGAGCAG CTCCGCCAGC AGCTGTCCCC GGAGAGGCAG 
CCCCAGATCA GCGGCCCCGA CCGCCTGGCC TACAGCAGCC AGACGGCGCA GCAGAAGGTG 
ACCCAGTGGG CCGAGGAGAG GGACGAACTC TTTCGCCAAA GTCTGGCAAA ACTTCGGGAG 
CAGCTGGTGA AAGCCAACAC CCTGGTGAGA GAGGCAAACT TCTTAGCCGA GGAGATGAGC 
AAGCTCACCG ACTACCAGGT GACTCTGCAG ATCCCTGCTG CGAACCTCAG TGCCAACAGG 
AAGAGAGGAG CAATAGTGAG TGAGCCAGCT ATTCAAGTGA GGAGGAAAGG AAAGAGCACC 
CAAGTGTGGA CCATTGAGAA GCTGGAGAAT AAATTAATTG ATATGAGAGA CCTTTATCAA 
GAATGGAAAG AAAAGATTCC TGAGGCAAAG CGACTCTGCG GGAAACGAGG TGACCCTTTC 
TATGAAGCCC AAGAGAATCA CAACCTGATT GGCGTGGCGA ATGTGTTCCT GGAGTGTCTC 
TTCTGTGATG TGAAGCTTCA GTATGCAGTT CCTATCATCA GCCAGCAGGG GGAGGTTGCA 
GGGCGTCTCC ACGTGGAAGT GATGCGAGTG ACAGGCGCTG TCCCGGAGCG CATGGCGGAG 
GACGACTCCT CGGAGAACTC CAGTGAAAGC GGAAGTCTTG AAGTCGTAGA CAGCACCGGG 
GAAATCATTC ACCGAGTCAA GAAGCTGACA TGCCGGGTAA AAATTAAAGA AGCAACTGGG 
CTGCCCTTAA ACCTCTCAAA TTTCGTCTTC TGTCAATACA CGTTCTGGGA CCAGTGTGAG 
CCCACGGTGG CCGCCCCTGT CGTGGATCCT GAGGTGCCTT CCCCACAGTC CAAGGACGCC 
CAGTACACCG TGACCTTCTC CCACTGTCAG GATTACGTGG TCAACGTAAC GGAAGAATTC 
CTGGAGTTCA TTTCAGATGG AGCACTGGCC ATCGAAGTGT GGGGCCACCG GTGTACCGGG 
AACGGCAGCT CCATCTGGGA AGTCGATTCC CTTCATGCTA AGACGAGAAC GCTGCATGAC 
AGGTGGAATG AAGTTACTCG CAGAATAGAG ATGTGGATCT CCATCCTGGA GCTGAACGAG 
CTGGGGGAGT ACGCCGCCGT GGAGCTTCAC CAGGCCAAGG ACGTCAACAC AGGCGGCGTC 
CTTCAGCTCA GACAGGGTCA TTCCCGGAGA GTCCAAGTCA CAGTGAAGCC TGTCCAGCAT 
TCCGGGACAC TGCCACTCAT GGTTGAAGCC ATCTTGTCCA TCTCCATCGG CTGCATCACT 
GCCAGGTCAA CCAAGCTCCA GAGAGGGCTG GACAGTTACC AGAGAGATGA TGAGGATGGT 
GATGATATGG ATAGTTATCA GGAAGAAGAC TTAAACTGCG TGAGAGAGAG GTGGTCGGAT 
GCCCTCATTA AACGGCGAGA ATACCTAGAC GAACAGATTA AAAAAGTCAG CAACAAAAAA 
GAGAAGACGG AGGATGACGT GGAGCGGGAA GCCCGGCTGG TGGAGCAGTG GGTGGGGCTG 
ACGGAGGAGA GGAACGCTGT GCTGGTGCCT GCGCCGGGCA GCGGGATCCC CGGGGCGCCT 
GCCGACTGGA TCCCACCACC TGGAATGGAA ACCCACATAC CGGTTCTCTT CCTTGATTTG 
AACGCGGATG ACCTCAGTGC CAATGAGCAG CTCGTGGGCC CCCACGCATC AGGCGTGAAC 
TCCATCCTCC CCAAGGAGCA CGGCAGCCAG TTTTTCTACC TGCCCATCAT AAAGCACAGT 
GACGAGGAGG TTTCAGCCAC AGCCTCCTGG GACTCCTCGG TGCATGATTC CGTTCACTTG 
AACAGGGTCA CCCCGCAGAA TGAGAGGATC TACCTGATAG TGAAGACCAC AGTCCAGCTC 
AGCCACCCGG CCGCTATGGA GCTGGTCTTA CGAAAACGGA TTGCAGCCAA CATTTACAAC 
AAACAGAGTT TCACCCAGAG TTTGAAGAGG AGAATATCAC TGAAGAATAT ATTTTATTCC 
TGTGGTGTAA CCTATGAAAT AGTATCCAAC ATACCAAAGG CAACAGAGGA GATTGAGGAC 
CGGGAGACGC TGGCTCTCAT GGCTGCAAGG AGTGAGAATG AAGGCACGTC GGATGGGGAG 
ACGTACATCG AGAAGTACAC GCGGGGCGTG CTGCAGGTGG AGAACATTCT GAGCCTGGAA 
CGACTCCGGC AGGCGGTGAC GGTCAAAGAA GCACTTTCCA CCAAAGCTCG GCACCTACGG 
CGAAGCCTCA GCACGCCCAA CGTTCACAAT GTCTCTTCCA GTCGACCAGA CCTTTCTGGC 
TTTGACGATG ATGATAAGGG TTGGCCAGAG AACCAGTTAG ACATGTCTGA CTACAGCCCG 
AGTTACCAGG ATGTATCGTG TTATGGAACT TTGCCTAGGG ATTCACCTCG AAGGAGTAAA 
GAAGGTTGTA CATCAGAGAA TCCTCATGCC TTAACAGTCA GCCCTTTCAA AGCATTCTCT 
CCTCAGCCAC CAAAGTTTTT CAAGCCCCTA ATGCCTGTAA AAGAGGAGCA TAAGAAAAGG 
ATAGCGCTTG AAGCAAGGCC TCTTCTAAGC CAGGAGGACT CTGAGGAGGA GGAAAATGAT 
CTAGAAGGTC TCAGCCGGAA GCTGATAAGT TCACAGCCTT ATGTACCTGT GGAGTTCGCT 
GACTTCAGCG TTTACAATGC CAGCTTGGAG AACAGAGAGT GGTTTTCTTC TAAAGGCGAT 
CTGACGAACT CAAAGGTCCT AGAGAAGGAG GTGTCCCGCA GCCCCACCAC CAGCAGCATC 
ACCAGCGGCT ACTTTTCCCA CAGTGCCTCC AACGCCACCC TGTCCGACAT GGTGGTCCCT 
TCCAGTGACA GCTCAGATCA GTTGGCCCTT CCAACGAAGG ACACAGATTC CAGCGAGCAT 
CCCGGACCGT CCCTTGGGCA GGATTTCAGA CCATCTTCAA ACAAAGAGTT GACAGAACTA 
GAAAGAGGCT TGGGAAAGGA TAAGATGACC GTGGTGCCAC TCAAGGAAAA CAGTGCCTTA 
GCCAAAGGGA GCCCATTGTC CCCCAGTATC CCGGAGAAAA ACTCCAGAAC CCTCTGTAGG 
ACCAGCTCGT GTTCAGAGCA GGATGCCTGC TCCAGCAAAA ACGGCCAGCC GGCCAGGGAA 
TTCTGCCCCA GGGAGGTGAC CGTGGAACAC ACCACAAACA TCCTCGAGGA TCACTCTTTC 
ACAGAGTTTA TGGGTGTGTC AGATGGGAAA GATTTTGATG GTTTGACGGA TTCTTCTGCT 
GGAGAACTTT CAAGTAGGAG GAACCTACCA AATACAGCGG ACAGTAGGAG TTTCTCACAT 
GGGCCACAGG ACCCTGGCCA GCTGTGTTCC TCGGCAGAGA ATGACCAGGG GAGCGGCACT 
GCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_024983947.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839715.1 Bos taurus kinesin family member 13A (KIF13A), transcript variant X6, mRNA.

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CloneID	OBa229714
Clone ID Related Accession (Same CDS sequence)	XM_024983947.1
Accession Version	XM_024983947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGATA CCAAGGTAAA AGTTGCCGTC CGGGTCCGGC CCCTGAACCG ACGAGAACTG 
GAACTCAACA CCAAGTGCGT GGTGGAGATG GAAGGGAATC AAACGGTCCT GCACCCTCCT 
CCTTCTAACA CCAAACAGGG AGAAAGGAAA CCTCCCAAGG TATTTGCATT TGATTATTGC 
TTTTGGTCCA TGGATGAATC CAACACTACA AAGTATGCTG GTCAAGAAGT GGTTTTCAAG 
TGCCTTGGGG AAGGAATTCT TGAAAAAGCC TTTCAGGGGT ATAATGCTTG CATTTTTGCA 
TATGGACAGA CAGGCTCAGG AAAATCCTTC TCCATGATGG GCAATGCTGA GCAGCGAGGC 
CTCATCCCAA GGCTCTGCTG TGCTTTATTT CAAAGGATCT CTCTGGAGCA GAATGAGTCA 
CAGACATTTA AAGTGGAAGT ATCCTATATG GAAATTTATA ATGAGAAAGT CCGGGACCTG 
TTAGACCCCA AAGGGAGTAG ACAGTCTCTT AAAGTTCGAG AGCATAAAGT TTTGGGACCA 
TATGTAGATG GTTTATCCCA ACTGGCTGTC ACTAGTTTTG AGGACATTGA GTCATTGATG 
TCTGAGGGAA ACAAATCTCG GACGGTAGCT GCGACCAACA TGAATGAAGA AAGCAGCCGC 
TCCCATGCAG TGTTCAACAT CGTCATCACG CAGACGCTGT ACGACCTGCA GTCTGGGAAC 
TCAGGGGAGA AGGTGAGCAA GGTGAGCTTG GTGGACCTGG CGGGCAGCGA GAGAGTGTCT 
AAAACTGGAG CTGCGGGAGA GCGCCTGAAA GAAGGCAGCA ACATTAACAA ATCACTTACA 
ACCTTGGGGT TGGTTATCTC ATCGCTGGCT GACCAGGCAG CTGGCAAGGG TAAAAACAAA 
TTTGTGCCTT ATCGCGACTC AGTCCTCACT TGGCTGCTTA AGGACAATCT GGGGGGCAAC 
AGCCAGACCT CCATGATCGC CACCATTAGC CCAGCAGCAG ACAACTACGA AGAGACCCTC 
TCCACGCTGA GATACGCAGA TCGGGCCAAA AGGATCGTTA ACCATGCCGT CGTGAATGAG 
GATCCCAACG CGAAGGTCAT CCGGGAACTG CGGGAAGAGG TGGAGAAACT GAGGGAGCAG 
CTCTCACAGG CTGAGGCTAT GAAGGCCCCT GAACTGAAAG AGAAGCTGGA AGAGTCTGAA 
AAGCTGATAA AAGAGCTAAC AGTGACTTGG GAAGAGAAGC TCAGGAAAAC AGAAGAAATT 
GCACAGGAGA GGCAGCGGCA ACTTGAAAGC ATGGGGATCT CCCTGGAGAC TTCTGGTATC 
AAAGTAGGAG ATGACAAGTG CTACTTGGTC AACCTGAATG CAGACCCTGC TCTCAACGAA 
CTTCTGGTTT ATTATTTAAA GGACCACACC AGGGTAGGTG CCGACACCTC TCAGGACATC 
CAGCTCTTTG GGATAGGAAT TCAGCCTGAG CACTGTGAGA TCGACATTGG CTCTGATGGA 
GAGGTCAGCC TCACTCCAAA AGAAAATGCA CGGTCCTGTG TCAATGGCAC CCTCGTGTGC 
AGCACCACCC AGCTGTGGCA TGGGGACCGA ATCCTGTGGG GAAACAACCA CTTCTTCAGG 
ATTAATTTGC CCAAGAGGAA ACGGCGAGAT TGGTTGAAAG ACTTTGAGAA AGAGACAGGC 
CCGCCGGAGC ACGACCTGGA CGCGGCCAGT GAAGCGTCCT CAGAACCAGA CTATAACTAC 
GAATTTGCGC AGATGGAGGT TATCATGAAA ACGCTGAATA GCAATGACCC AGTTCAGAAC 
GTGGTGCAGG TCTTGGAGAA ACAGTACCTG GAAGAAAAGC GAAGCGCCCT CGAGGAGCAG 
CGACTCATGT ACGAGCGGGA GCTGGAGCAG CTCCGCCAGC AGCTGTCCCC GGAGAGGCAG 
CCCCAGATCA GCGGCCCCGA CCGCCTGGCC TACAGCAGCC AGACGGCGCA GCAGAAGGTG 
ACCCAGTGGG CCGAGGAGAG GGACGAACTC TTTCGCCAAA GTCTGGCAAA ACTTCGGGAG 
CAGCTGGTGA AAGCCAACAC CCTGGTGAGA GAGGCAAACT TCTTAGCCGA GGAGATGAGC 
AAGCTCACCG ACTACCAGGT GACTCTGCAG ATCCCTGCTG CGAACCTCAG TGCCAACAGG 
AAGAGAGGAG CAATAGTGAG TGAGCCAGCT ATTCAAGTGA GGAGGAAAGG AAAGAGCACC 
CAAGTGTGGA CCATTGAGAA GCTGGAGAAT AAATTAATTG ATATGAGAGA CCTTTATCAA 
GAATGGAAAG AAAAGATTCC TGAGGCAAAG CGACTCTGCG GGAAACGAGG TGACCCTTTC 
TATGAAGCCC AAGAGAATCA CAACCTGATT GGCGTGGCGA ATGTGTTCCT GGAGTGTCTC 
TTCTGTGATG TGAAGCTTCA GTATGCAGTT CCTATCATCA GCCAGCAGGG GGAGGTTGCA 
GGGCGTCTCC ACGTGGAAGT GATGCGAGTG ACAGGCGCTG TCCCGGAGCG CATGGCGGAG 
GACGACTCCT CGGAGAACTC CAGTGAAAGC GGAAGTCTTG AAGTCGTAGA CAGCACCGGG 
GAAATCATTC ACCGAGTCAA GAAGCTGACA TGCCGGGTAA AAATTAAAGA AGCAACTGGG 
CTGCCCTTAA ACCTCTCAAA TTTCGTCTTC TGTCAATACA CGTTCTGGGA CCAGTGTGAG 
CCCACGGTGG CCGCCCCTGT CGTGGATCCT GAGGTGCCTT CCCCACAGTC CAAGGACGCC 
CAGTACACCG TGACCTTCTC CCACTGTCAG GATTACGTGG TCAACGTAAC GGAAGAATTC 
CTGGAGTTCA TTTCAGATGG AGCACTGGCC ATCGAAGTGT GGGGCCACCG GTGTACCGGG 
AACGGCAGCT CCATCTGGGA AGTCGATTCC CTTCATGCTA AGACGAGAAC GCTGCATGAC 
AGGTGGAATG AAGTTACTCG CAGAATAGAG ATGTGGATCT CCATCCTGGA GCTGAACGAG 
CTGGGGGAGT ACGCCGCCGT GGAGCTTCAC CAGGCCAAGG ACGTCAACAC AGGCGGCGTC 
CTTCAGCTCA GACAGGGTCA TTCCCGGAGA GTCCAAGTCA CAGTGAAGCC TGTCCAGCAT 
TCCGGGACAC TGCCACTCAT GGTTGAAGCC ATCTTGTCCA TCTCCATCGG CTGCATCACT 
GCCAGGTCAA CCAAGCTCCA GAGAGGGCTG GACAGTTACC AGGAAGAAGA CTTAAACTGC 
GTGAGAGAGA GGTGGTCGGA TGCCCTCATT AAACGGCGAG AATACCTAGA CGAACAGATT 
AAAAAAGTCA GCAACAAAAA AGAGAAGACG GAGGATGACG TGGAGCGGGA AGCCCGGCTG 
GTGGAGCAGT GGGTGGGGCT GACGGAGGAG AGGAACGCTG TGCTGGTGCC TGCGCCGGGC 
AGCGGGATCC CCGGGGCGCC TGCCGACTGG ATCCCACCAC CTGGAATGGA AACCCACATA 
CCGGTTCTCT TCCTTGATTT GAACGCGGAT GACCTCAGTG CCAATGAGCA GCTCGTGGGC 
CCCCACGCAT CAGGCGTGAA CTCCATCCTC CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC 
CTGCCCATCA TAAAGCACAG TGACGAGGAG GTTTCAGCCA CAGCCTCCTG GGACTCCTCG 
GTGCATGATT CCGTTCACTT GAACAGGGTC ACCCCGCAGA ATGAGAGGAT CTACCTGATA 
GTGAAGACCA CAGTCCAGCT CAGCCACCCG GCCGCTATGG AGCTGGTCTT ACGAAAACGG 
ATTGCAGCCA ACATTTACAA CAAACAGAGT TTCACCCAGA GTTTGAAGAG GAGAATATCA 
CTGAAGAATA TATTTTATTC CTGTGGTGTA ACCTATGAAA TAGTATCCAA CATACCAAAG 
GCAACAGAGG AGATTGAGGA CCGGGAGACG CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT 
GAAGGCACGT CGGATGGGGA GACGTACATC GAGAAGTACA CGCGGGGCGT GCTGCAGGTG 
GAGAACATTC TGAGCCTGGA ACGACTCCGG CAGGCGGTGA CGGTCAAAGA AGCACTTTCC 
ACCAAAGCTC GGCACCTACG GCGAAGCCTC AGCACGCCCA ACGTTCACAA TGTCTCTTCC 
AGTCGACCAG ACCTTTCTGG CTTTGACGAT GATGATAAGG GTTGGCCAGA GAACCAGTTA 
GACATGTCTG ACTACAGCCC GAGTTACCAG GATGTATCGT GTTATGGAAC TTTGCCTAGG 
GATTCACCTC GAAGGAGTAA AGAAGGTTGT ACATCAGAGA ATCCTCATGC CTTAACAGTC 
AGCCCTTTCA AAGCATTCTC TCCTCAGCCA CCAAAGTTTT TCAAGCCCCT AATGCCTGTA 
AAAGAGGAGC ATAAGAAAAG GATAGCGCTT GAAGCAAGGC CTCTTCTAAG CCAGGAGGAC 
TCTGAGGAGG AGGAAAATGA TCTAGAAGGT CTCAGCCGGA AGCTGATAAG TTCACAGCCT 
TATGTACCTG TGGAGTTCGC TGACTTCAGC GTTTACAATG CCAGCTTGGA GAACAGAGAG 
TGGTTTTCTT CTAAAGGCGA TCTGACGAAC TCAAAGGTCC TAGAGAAGGA GGTGTCCCGC 
AGCCCCACCA CCAGCAGCAT CACCAGCGGC TACTTTTCCC ACAGTGCCTC CAACGCCACC 
CTGTCCGACA TGGTGGTCCC TTCCAGTGAC AGCTCAGATC AGTTGGCCCT TCCAACGAAG 
GACACAGATT CCAGCGAGCA TCCCGGACCG TCCCTTGGGC AGGATTTCAG ACCATCTTCA 
AACAAAGAGT TGACAGAACT AGAAAGAGGC TTGGGAAAGG ATAAGATGAC CGTGGTGCCA 
CTCAAGGAAA ACAGTGCCTT AGCCAAAGGG AGCCCATTGT CCCCCAGTAT CCCGGAGAAA 
AACTCCAGAA CCCTCTGTAG GACCAGCTCG TGTTCAGAGC AGGATGCCTG CTCCAGCAAA 
AACGGCCAGC CGGCCAGGGA ATTCTGCCCC AGGGAGGTGA CCGTGGAACA CACCACAAAC 
ATCCTCGAGG ATCACTCTTT CACAGAGTTT ATGGGTGTGT CAGATGGGAA AGATTTTGAT 
GGTTTGACGG ATTCTTCTGC TGGAGAACTT TCAAGTAGGA GGAACCTACC AAATACAGCG 
GACAGTAGGA GTTTCTCACA TGGGCCACAG GACCCTGGCC AGCTGTGTTC CTCGGCAGAG 
AATGACCAGG GGAGCGGCAC TGCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839715.1
CDS	1..5250
Translation	MSDTKVKVAVRVRPLNRRELELNTKCVVEMEGNQTVLHPPPSNTKQGERKPPKVFAFDYCFWSMDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQEDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_024983947.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 13A (KIF13A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983947.1

ORF Insert Sequence:

ATGTCGGATA CCAAGGTAAA AGTTGCCGTC CGGGTCCGGC CCCTGAACCG ACGAGAACTG 
GAACTCAACA CCAAGTGCGT GGTGGAGATG GAAGGGAATC AAACGGTCCT GCACCCTCCT 
CCTTCTAACA CCAAACAGGG AGAAAGGAAA CCTCCCAAGG TATTTGCATT TGATTATTGC 
TTTTGGTCCA TGGATGAATC CAACACTACA AAGTATGCTG GTCAAGAAGT GGTTTTCAAG 
TGCCTTGGGG AAGGAATTCT TGAAAAAGCC TTTCAGGGGT ATAATGCTTG CATTTTTGCA 
TATGGACAGA CAGGCTCAGG AAAATCCTTC TCCATGATGG GCAATGCTGA GCAGCGAGGC 
CTCATCCCAA GGCTCTGCTG TGCTTTATTT CAAAGGATCT CTCTGGAGCA GAATGAGTCA 
CAGACATTTA AAGTGGAAGT ATCCTATATG GAAATTTATA ATGAGAAAGT CCGGGACCTG 
TTAGACCCCA AAGGGAGTAG ACAGTCTCTT AAAGTTCGAG AGCATAAAGT TTTGGGACCA 
TATGTAGATG GTTTATCCCA ACTGGCTGTC ACTAGTTTTG AGGACATTGA GTCATTGATG 
TCTGAGGGAA ACAAATCTCG GACGGTAGCT GCGACCAACA TGAATGAAGA AAGCAGCCGC 
TCCCATGCAG TGTTCAACAT CGTCATCACG CAGACGCTGT ACGACCTGCA GTCTGGGAAC 
TCAGGGGAGA AGGTGAGCAA GGTGAGCTTG GTGGACCTGG CGGGCAGCGA GAGAGTGTCT 
AAAACTGGAG CTGCGGGAGA GCGCCTGAAA GAAGGCAGCA ACATTAACAA ATCACTTACA 
ACCTTGGGGT TGGTTATCTC ATCGCTGGCT GACCAGGCAG CTGGCAAGGG TAAAAACAAA 
TTTGTGCCTT ATCGCGACTC AGTCCTCACT TGGCTGCTTA AGGACAATCT GGGGGGCAAC 
AGCCAGACCT CCATGATCGC CACCATTAGC CCAGCAGCAG ACAACTACGA AGAGACCCTC 
TCCACGCTGA GATACGCAGA TCGGGCCAAA AGGATCGTTA ACCATGCCGT CGTGAATGAG 
GATCCCAACG CGAAGGTCAT CCGGGAACTG CGGGAAGAGG TGGAGAAACT GAGGGAGCAG 
CTCTCACAGG CTGAGGCTAT GAAGGCCCCT GAACTGAAAG AGAAGCTGGA AGAGTCTGAA 
AAGCTGATAA AAGAGCTAAC AGTGACTTGG GAAGAGAAGC TCAGGAAAAC AGAAGAAATT 
GCACAGGAGA GGCAGCGGCA ACTTGAAAGC ATGGGGATCT CCCTGGAGAC TTCTGGTATC 
AAAGTAGGAG ATGACAAGTG CTACTTGGTC AACCTGAATG CAGACCCTGC TCTCAACGAA 
CTTCTGGTTT ATTATTTAAA GGACCACACC AGGGTAGGTG CCGACACCTC TCAGGACATC 
CAGCTCTTTG GGATAGGAAT TCAGCCTGAG CACTGTGAGA TCGACATTGG CTCTGATGGA 
GAGGTCAGCC TCACTCCAAA AGAAAATGCA CGGTCCTGTG TCAATGGCAC CCTCGTGTGC 
AGCACCACCC AGCTGTGGCA TGGGGACCGA ATCCTGTGGG GAAACAACCA CTTCTTCAGG 
ATTAATTTGC CCAAGAGGAA ACGGCGAGAT TGGTTGAAAG ACTTTGAGAA AGAGACAGGC 
CCGCCGGAGC ACGACCTGGA CGCGGCCAGT GAAGCGTCCT CAGAACCAGA CTATAACTAC 
GAATTTGCGC AGATGGAGGT TATCATGAAA ACGCTGAATA GCAATGACCC AGTTCAGAAC 
GTGGTGCAGG TCTTGGAGAA ACAGTACCTG GAAGAAAAGC GAAGCGCCCT CGAGGAGCAG 
CGACTCATGT ACGAGCGGGA GCTGGAGCAG CTCCGCCAGC AGCTGTCCCC GGAGAGGCAG 
CCCCAGATCA GCGGCCCCGA CCGCCTGGCC TACAGCAGCC AGACGGCGCA GCAGAAGGTG 
ACCCAGTGGG CCGAGGAGAG GGACGAACTC TTTCGCCAAA GTCTGGCAAA ACTTCGGGAG 
CAGCTGGTGA AAGCCAACAC CCTGGTGAGA GAGGCAAACT TCTTAGCCGA GGAGATGAGC 
AAGCTCACCG ACTACCAGGT GACTCTGCAG ATCCCTGCTG CGAACCTCAG TGCCAACAGG 
AAGAGAGGAG CAATAGTGAG TGAGCCAGCT ATTCAAGTGA GGAGGAAAGG AAAGAGCACC 
CAAGTGTGGA CCATTGAGAA GCTGGAGAAT AAATTAATTG ATATGAGAGA CCTTTATCAA 
GAATGGAAAG AAAAGATTCC TGAGGCAAAG CGACTCTGCG GGAAACGAGG TGACCCTTTC 
TATGAAGCCC AAGAGAATCA CAACCTGATT GGCGTGGCGA ATGTGTTCCT GGAGTGTCTC 
TTCTGTGATG TGAAGCTTCA GTATGCAGTT CCTATCATCA GCCAGCAGGG GGAGGTTGCA 
GGGCGTCTCC ACGTGGAAGT GATGCGAGTG ACAGGCGCTG TCCCGGAGCG CATGGCGGAG 
GACGACTCCT CGGAGAACTC CAGTGAAAGC GGAAGTCTTG AAGTCGTAGA CAGCACCGGG 
GAAATCATTC ACCGAGTCAA GAAGCTGACA TGCCGGGTAA AAATTAAAGA AGCAACTGGG 
CTGCCCTTAA ACCTCTCAAA TTTCGTCTTC TGTCAATACA CGTTCTGGGA CCAGTGTGAG 
CCCACGGTGG CCGCCCCTGT CGTGGATCCT GAGGTGCCTT CCCCACAGTC CAAGGACGCC 
CAGTACACCG TGACCTTCTC CCACTGTCAG GATTACGTGG TCAACGTAAC GGAAGAATTC 
CTGGAGTTCA TTTCAGATGG AGCACTGGCC ATCGAAGTGT GGGGCCACCG GTGTACCGGG 
AACGGCAGCT CCATCTGGGA AGTCGATTCC CTTCATGCTA AGACGAGAAC GCTGCATGAC 
AGGTGGAATG AAGTTACTCG CAGAATAGAG ATGTGGATCT CCATCCTGGA GCTGAACGAG 
CTGGGGGAGT ACGCCGCCGT GGAGCTTCAC CAGGCCAAGG ACGTCAACAC AGGCGGCGTC 
CTTCAGCTCA GACAGGGTCA TTCCCGGAGA GTCCAAGTCA CAGTGAAGCC TGTCCAGCAT 
TCCGGGACAC TGCCACTCAT GGTTGAAGCC ATCTTGTCCA TCTCCATCGG CTGCATCACT 
GCCAGGTCAA CCAAGCTCCA GAGAGGGCTG GACAGTTACC AGGAAGAAGA CTTAAACTGC 
GTGAGAGAGA GGTGGTCGGA TGCCCTCATT AAACGGCGAG AATACCTAGA CGAACAGATT 
AAAAAAGTCA GCAACAAAAA AGAGAAGACG GAGGATGACG TGGAGCGGGA AGCCCGGCTG 
GTGGAGCAGT GGGTGGGGCT GACGGAGGAG AGGAACGCTG TGCTGGTGCC TGCGCCGGGC 
AGCGGGATCC CCGGGGCGCC TGCCGACTGG ATCCCACCAC CTGGAATGGA AACCCACATA 
CCGGTTCTCT TCCTTGATTT GAACGCGGAT GACCTCAGTG CCAATGAGCA GCTCGTGGGC 
CCCCACGCAT CAGGCGTGAA CTCCATCCTC CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC 
CTGCCCATCA TAAAGCACAG TGACGAGGAG GTTTCAGCCA CAGCCTCCTG GGACTCCTCG 
GTGCATGATT CCGTTCACTT GAACAGGGTC ACCCCGCAGA ATGAGAGGAT CTACCTGATA 
GTGAAGACCA CAGTCCAGCT CAGCCACCCG GCCGCTATGG AGCTGGTCTT ACGAAAACGG 
ATTGCAGCCA ACATTTACAA CAAACAGAGT TTCACCCAGA GTTTGAAGAG GAGAATATCA 
CTGAAGAATA TATTTTATTC CTGTGGTGTA ACCTATGAAA TAGTATCCAA CATACCAAAG 
GCAACAGAGG AGATTGAGGA CCGGGAGACG CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT 
GAAGGCACGT CGGATGGGGA GACGTACATC GAGAAGTACA CGCGGGGCGT GCTGCAGGTG 
GAGAACATTC TGAGCCTGGA ACGACTCCGG CAGGCGGTGA CGGTCAAAGA AGCACTTTCC 
ACCAAAGCTC GGCACCTACG GCGAAGCCTC AGCACGCCCA ACGTTCACAA TGTCTCTTCC 
AGTCGACCAG ACCTTTCTGG CTTTGACGAT GATGATAAGG GTTGGCCAGA GAACCAGTTA 
GACATGTCTG ACTACAGCCC GAGTTACCAG GATGTATCGT GTTATGGAAC TTTGCCTAGG 
GATTCACCTC GAAGGAGTAA AGAAGGTTGT ACATCAGAGA ATCCTCATGC CTTAACAGTC 
AGCCCTTTCA AAGCATTCTC TCCTCAGCCA CCAAAGTTTT TCAAGCCCCT AATGCCTGTA 
AAAGAGGAGC ATAAGAAAAG GATAGCGCTT GAAGCAAGGC CTCTTCTAAG CCAGGAGGAC 
TCTGAGGAGG AGGAAAATGA TCTAGAAGGT CTCAGCCGGA AGCTGATAAG TTCACAGCCT 
TATGTACCTG TGGAGTTCGC TGACTTCAGC GTTTACAATG CCAGCTTGGA GAACAGAGAG 
TGGTTTTCTT CTAAAGGCGA TCTGACGAAC TCAAAGGTCC TAGAGAAGGA GGTGTCCCGC 
AGCCCCACCA CCAGCAGCAT CACCAGCGGC TACTTTTCCC ACAGTGCCTC CAACGCCACC 
CTGTCCGACA TGGTGGTCCC TTCCAGTGAC AGCTCAGATC AGTTGGCCCT TCCAACGAAG 
GACACAGATT CCAGCGAGCA TCCCGGACCG TCCCTTGGGC AGGATTTCAG ACCATCTTCA 
AACAAAGAGT TGACAGAACT AGAAAGAGGC TTGGGAAAGG ATAAGATGAC CGTGGTGCCA 
CTCAAGGAAA ACAGTGCCTT AGCCAAAGGG AGCCCATTGT CCCCCAGTAT CCCGGAGAAA 
AACTCCAGAA CCCTCTGTAG GACCAGCTCG TGTTCAGAGC AGGATGCCTG CTCCAGCAAA 
AACGGCCAGC CGGCCAGGGA ATTCTGCCCC AGGGAGGTGA CCGTGGAACA CACCACAAAC 
ATCCTCGAGG ATCACTCTTT CACAGAGTTT ATGGGTGTGT CAGATGGGAA AGATTTTGAT 
GGTTTGACGG ATTCTTCTGC TGGAGAACTT TCAAGTAGGA GGAACCTACC AAATACAGCG 
GACAGTAGGA GTTTCTCACA TGGGCCACAG GACCCTGGCC AGCTGTGTTC CTCGGCAGAG 
AATGACCAGG GGAGCGGCAC TGCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_010818550.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816852.1 Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X4, mRNA.

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CloneID	OBa32275
Clone ID Related Accession (Same CDS sequence)	XM_010818550.2 , XM_015469028.1
Accession Version	XM_010818550.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104561.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818550.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGATT CTGAAGTTAA TCTACCTTGA GGAATTGCAC 
TTACTTGGAG ACTCTGAGGA GGAGGAAAAT GATCTAGAAG GTCTCAGCCG GAAGCTGATA 
AGTTCACAGC CTTATGTACC TGTGGAGTTC GCTGACTTCA GCGTTTACAA TGCCAGCTTG 
GAGAACAGAG AGTGGTTTTC TTCTAAAGGC GATCTGACGA ACTCAAAGGT CCTAGAGAAG 
GAGGTGTCCC GCAGCCCCAC CACCAGCAGC ATCACCAGCG GCTACTTTTC CCACAGTGCC 
TCCAACGCCA CCCTGTCCGA CATGGTGGTC CCTTCCAGTG ACAGCTCAGA TCAGTTGGCC 
CTTCCAACGA AGGACACAGA TTCCAGCGAG CATCCCGGAC CGTCCCTTGG GCAGGATTTC 
AGACCATCTT CAAACAAAGA GTTGACAGAA CTAGAAAGAG GCTTGGGAAA GGATAAGATG 
ACCGTGGTGC CACTCAAGGA AAACAGTGCC TTAGCCAAAG GGAGCCCATT GTCCCCCAGT 
ATCCCGGAGA AAAACTCCAG AACCCTCTGT AGGACCAGCT CGTGTTCAGA GCAGGATGCC 
TGCTCCAGCA AAAACGGCCA GCCGGCCAGG GAATTCTGCC CCAGGGAGGT GACCGTGGAA 
CACACCACAA ACATCCTCGA GGATCACTCT TTCACAGAGT TTATGGGTGT GTCAGATGGG 
AAAGATTTTG ATGGTTTGAC GGATTCTTCT GCTGGAGAAC TTTCAAGTAG GAGGAACCTA 
CCAAATACAG CGGACAGTAG GAGTTTCTCA CATGGGCCAC AGGACCCTGG CCAGCTGTGT 
TCCTCGGCAG AGAATGACCA GGGGAGCGGC ACTGCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816852.1
CDS	31..5292
Translation	MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQEILKLIYLEELHLLGDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_010818550.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818550.2

ORF Insert Sequence:

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGATT CTGAAGTTAA TCTACCTTGA GGAATTGCAC 
TTACTTGGAG ACTCTGAGGA GGAGGAAAAT GATCTAGAAG GTCTCAGCCG GAAGCTGATA 
AGTTCACAGC CTTATGTACC TGTGGAGTTC GCTGACTTCA GCGTTTACAA TGCCAGCTTG 
GAGAACAGAG AGTGGTTTTC TTCTAAAGGC GATCTGACGA ACTCAAAGGT CCTAGAGAAG 
GAGGTGTCCC GCAGCCCCAC CACCAGCAGC ATCACCAGCG GCTACTTTTC CCACAGTGCC 
TCCAACGCCA CCCTGTCCGA CATGGTGGTC CCTTCCAGTG ACAGCTCAGA TCAGTTGGCC 
CTTCCAACGA AGGACACAGA TTCCAGCGAG CATCCCGGAC CGTCCCTTGG GCAGGATTTC 
AGACCATCTT CAAACAAAGA GTTGACAGAA CTAGAAAGAG GCTTGGGAAA GGATAAGATG 
ACCGTGGTGC CACTCAAGGA AAACAGTGCC TTAGCCAAAG GGAGCCCATT GTCCCCCAGT 
ATCCCGGAGA AAAACTCCAG AACCCTCTGT AGGACCAGCT CGTGTTCAGA GCAGGATGCC 
TGCTCCAGCA AAAACGGCCA GCCGGCCAGG GAATTCTGCC CCAGGGAGGT GACCGTGGAA 
CACACCACAA ACATCCTCGA GGATCACTCT TTCACAGAGT TTATGGGTGT GTCAGATGGG 
AAAGATTTTG ATGGTTTGAC GGATTCTTCT GCTGGAGAAC TTTCAAGTAG GAGGAACCTA 
CCAAATACAG CGGACAGTAG GAGTTTCTCA CATGGGCCAC AGGACCCTGG CCAGCTGTGT 
TCCTCGGCAG AGAATGACCA GGGGAGCGGC ACTGCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_015469028.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324514.1 Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X4, mRNA.

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CloneID	OBa32275
Clone ID Related Accession (Same CDS sequence)	XM_010818550.2 , XM_015469028.1
Accession Version	XM_015469028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646615.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGATT CTGAAGTTAA TCTACCTTGA GGAATTGCAC 
TTACTTGGAG ACTCTGAGGA GGAGGAAAAT GATCTAGAAG GTCTCAGCCG GAAGCTGATA 
AGTTCACAGC CTTATGTACC TGTGGAGTTC GCTGACTTCA GCGTTTACAA TGCCAGCTTG 
GAGAACAGAG AGTGGTTTTC TTCTAAAGGC GATCTGACGA ACTCAAAGGT CCTAGAGAAG 
GAGGTGTCCC GCAGCCCCAC CACCAGCAGC ATCACCAGCG GCTACTTTTC CCACAGTGCC 
TCCAACGCCA CCCTGTCCGA CATGGTGGTC CCTTCCAGTG ACAGCTCAGA TCAGTTGGCC 
CTTCCAACGA AGGACACAGA TTCCAGCGAG CATCCCGGAC CGTCCCTTGG GCAGGATTTC 
AGACCATCTT CAAACAAAGA GTTGACAGAA CTAGAAAGAG GCTTGGGAAA GGATAAGATG 
ACCGTGGTGC CACTCAAGGA AAACAGTGCC TTAGCCAAAG GGAGCCCATT GTCCCCCAGT 
ATCCCGGAGA AAAACTCCAG AACCCTCTGT AGGACCAGCT CGTGTTCAGA GCAGGATGCC 
TGCTCCAGCA AAAACGGCCA GCCGGCCAGG GAATTCTGCC CCAGGGAGGT GACCGTGGAA 
CACACCACAA ACATCCTCGA GGATCACTCT TTCACAGAGT TTATGGGTGT GTCAGATGGG 
AAAGATTTTG ATGGTTTGAC GGATTCTTCT GCTGGAGAAC TTTCAAGTAG GAGGAACCTA 
CCAAATACAG CGGACAGTAG GAGTTTCTCA CATGGGCCAC AGGACCCTGG CCAGCTGTGT 
TCCTCGGCAG AGAATGACCA GGGGAGCGGC ACTGCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324514.1
CDS	31..5292
Translation	MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQEILKLIYLEELHLLGDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_015469028.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469028.1

ORF Insert Sequence:

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGATT CTGAAGTTAA TCTACCTTGA GGAATTGCAC 
TTACTTGGAG ACTCTGAGGA GGAGGAAAAT GATCTAGAAG GTCTCAGCCG GAAGCTGATA 
AGTTCACAGC CTTATGTACC TGTGGAGTTC GCTGACTTCA GCGTTTACAA TGCCAGCTTG 
GAGAACAGAG AGTGGTTTTC TTCTAAAGGC GATCTGACGA ACTCAAAGGT CCTAGAGAAG 
GAGGTGTCCC GCAGCCCCAC CACCAGCAGC ATCACCAGCG GCTACTTTTC CCACAGTGCC 
TCCAACGCCA CCCTGTCCGA CATGGTGGTC CCTTCCAGTG ACAGCTCAGA TCAGTTGGCC 
CTTCCAACGA AGGACACAGA TTCCAGCGAG CATCCCGGAC CGTCCCTTGG GCAGGATTTC 
AGACCATCTT CAAACAAAGA GTTGACAGAA CTAGAAAGAG GCTTGGGAAA GGATAAGATG 
ACCGTGGTGC CACTCAAGGA AAACAGTGCC TTAGCCAAAG GGAGCCCATT GTCCCCCAGT 
ATCCCGGAGA AAAACTCCAG AACCCTCTGT AGGACCAGCT CGTGTTCAGA GCAGGATGCC 
TGCTCCAGCA AAAACGGCCA GCCGGCCAGG GAATTCTGCC CCAGGGAGGT GACCGTGGAA 
CACACCACAA ACATCCTCGA GGATCACTCT TTCACAGAGT TTATGGGTGT GTCAGATGGG 
AAAGATTTTG ATGGTTTGAC GGATTCTTCT GCTGGAGAAC TTTCAAGTAG GAGGAACCTA 
CCAAATACAG CGGACAGTAG GAGTTTCTCA CATGGGCCAC AGGACCCTGG CCAGCTGTGT 
TCCTCGGCAG AGAATGACCA GGGGAGCGGC ACTGCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_015469030.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324516.1 Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X6, mRNA.

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CloneID	OBa32276
Clone ID Related Accession (Same CDS sequence)	XM_015469030.1 , XM_010818552.2
Accession Version	XM_015469030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646615.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGGAC TCTGAGGAGG AGGAAAATGA TCTAGAAGGT 
CTCAGCCGGA AGCTGATAAG TTCACAGCCT TATGTACCTG TGGAGTTCGC TGACTTCAGC 
GTTTACAATG CCAGCTTGGA GAACAGAGAG TGGTTTTCTT CTAAAGGCGA TCTGACGAAC 
TCAAAGGTCC TAGAGAAGGA GGTGTCCCGC AGCCCCACCA CCAGCAGCAT CACCAGCGGC 
TACTTTTCCC ACAGTGCCTC CAACGCCACC CTGTCCGACA TGGTGGTCCC TTCCAGTGAC 
AGCTCAGATC AGTTGGCCCT TCCAACGAAG GACACAGATT CCAGCGAGCA TCCCGGACCG 
TCCCTTGGGC AGGATTTCAG ACCATCTTCA AACAAAGAGT TGACAGAACT AGAAAGAGGC 
TTGGGAAAGG ATAAGATGAC CGTGGTGCCA CTCAAGGAAA ACAGTGCCTT AGCCAAAGGG 
AGCCCATTGT CCCCCAGTAT CCCGGAGAAA AACTCCAGAA CCCTCTGTAG GACCAGCTCG 
TGTTCAGAGC AGGATGCCTG CTCCAGCAAA AACGGCCAGC CGGCCAGGGA ATTCTGCCCC 
AGGGAGGTGA CCGTGGAACA CACCACAAAC ATCCTCGAGG ATCACTCTTT CACAGAGTTT 
ATGGGTGTGT CAGATGGGAA AGATTTTGAT GGTTTGACGG ATTCTTCTGC TGGAGAACTT 
TCAAGTAGGA GGAACCTACC AAATACAGCG GACAGTAGGA GTTTCTCACA TGGGCCACAG 
GACCCTGGCC AGCTGTGTTC CTCGGCAGAG AATGACCAGG GGAGCGGCAC TGCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324516.1
CDS	31..5250
Translation	MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQEDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_015469030.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469030.1

ORF Insert Sequence:

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGGAC TCTGAGGAGG AGGAAAATGA TCTAGAAGGT 
CTCAGCCGGA AGCTGATAAG TTCACAGCCT TATGTACCTG TGGAGTTCGC TGACTTCAGC 
GTTTACAATG CCAGCTTGGA GAACAGAGAG TGGTTTTCTT CTAAAGGCGA TCTGACGAAC 
TCAAAGGTCC TAGAGAAGGA GGTGTCCCGC AGCCCCACCA CCAGCAGCAT CACCAGCGGC 
TACTTTTCCC ACAGTGCCTC CAACGCCACC CTGTCCGACA TGGTGGTCCC TTCCAGTGAC 
AGCTCAGATC AGTTGGCCCT TCCAACGAAG GACACAGATT CCAGCGAGCA TCCCGGACCG 
TCCCTTGGGC AGGATTTCAG ACCATCTTCA AACAAAGAGT TGACAGAACT AGAAAGAGGC 
TTGGGAAAGG ATAAGATGAC CGTGGTGCCA CTCAAGGAAA ACAGTGCCTT AGCCAAAGGG 
AGCCCATTGT CCCCCAGTAT CCCGGAGAAA AACTCCAGAA CCCTCTGTAG GACCAGCTCG 
TGTTCAGAGC AGGATGCCTG CTCCAGCAAA AACGGCCAGC CGGCCAGGGA ATTCTGCCCC 
AGGGAGGTGA CCGTGGAACA CACCACAAAC ATCCTCGAGG ATCACTCTTT CACAGAGTTT 
ATGGGTGTGT CAGATGGGAA AGATTTTGAT GGTTTGACGG ATTCTTCTGC TGGAGAACTT 
TCAAGTAGGA GGAACCTACC AAATACAGCG GACAGTAGGA GTTTCTCACA TGGGCCACAG 
GACCCTGGCC AGCTGTGTTC CTCGGCAGAG AATGACCAGG GGAGCGGCAC TGCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_010818552.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816854.1 Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X6, mRNA.

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CloneID	OBa32276
Clone ID Related Accession (Same CDS sequence)	XM_015469030.1 , XM_010818552.2
Accession Version	XM_010818552.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104561.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818552.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGGAC TCTGAGGAGG AGGAAAATGA TCTAGAAGGT 
CTCAGCCGGA AGCTGATAAG TTCACAGCCT TATGTACCTG TGGAGTTCGC TGACTTCAGC 
GTTTACAATG CCAGCTTGGA GAACAGAGAG TGGTTTTCTT CTAAAGGCGA TCTGACGAAC 
TCAAAGGTCC TAGAGAAGGA GGTGTCCCGC AGCCCCACCA CCAGCAGCAT CACCAGCGGC 
TACTTTTCCC ACAGTGCCTC CAACGCCACC CTGTCCGACA TGGTGGTCCC TTCCAGTGAC 
AGCTCAGATC AGTTGGCCCT TCCAACGAAG GACACAGATT CCAGCGAGCA TCCCGGACCG 
TCCCTTGGGC AGGATTTCAG ACCATCTTCA AACAAAGAGT TGACAGAACT AGAAAGAGGC 
TTGGGAAAGG ATAAGATGAC CGTGGTGCCA CTCAAGGAAA ACAGTGCCTT AGCCAAAGGG 
AGCCCATTGT CCCCCAGTAT CCCGGAGAAA AACTCCAGAA CCCTCTGTAG GACCAGCTCG 
TGTTCAGAGC AGGATGCCTG CTCCAGCAAA AACGGCCAGC CGGCCAGGGA ATTCTGCCCC 
AGGGAGGTGA CCGTGGAACA CACCACAAAC ATCCTCGAGG ATCACTCTTT CACAGAGTTT 
ATGGGTGTGT CAGATGGGAA AGATTTTGAT GGTTTGACGG ATTCTTCTGC TGGAGAACTT 
TCAAGTAGGA GGAACCTACC AAATACAGCG GACAGTAGGA GTTTCTCACA TGGGCCACAG 
GACCCTGGCC AGCTGTGTTC CTCGGCAGAG AATGACCAGG GGAGCGGCAC TGCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816854.1
CDS	31..5250
Translation	MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQEDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_010818552.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818552.2

ORF Insert Sequence:

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGGAC TCTGAGGAGG AGGAAAATGA TCTAGAAGGT 
CTCAGCCGGA AGCTGATAAG TTCACAGCCT TATGTACCTG TGGAGTTCGC TGACTTCAGC 
GTTTACAATG CCAGCTTGGA GAACAGAGAG TGGTTTTCTT CTAAAGGCGA TCTGACGAAC 
TCAAAGGTCC TAGAGAAGGA GGTGTCCCGC AGCCCCACCA CCAGCAGCAT CACCAGCGGC 
TACTTTTCCC ACAGTGCCTC CAACGCCACC CTGTCCGACA TGGTGGTCCC TTCCAGTGAC 
AGCTCAGATC AGTTGGCCCT TCCAACGAAG GACACAGATT CCAGCGAGCA TCCCGGACCG 
TCCCTTGGGC AGGATTTCAG ACCATCTTCA AACAAAGAGT TGACAGAACT AGAAAGAGGC 
TTGGGAAAGG ATAAGATGAC CGTGGTGCCA CTCAAGGAAA ACAGTGCCTT AGCCAAAGGG 
AGCCCATTGT CCCCCAGTAT CCCGGAGAAA AACTCCAGAA CCCTCTGTAG GACCAGCTCG 
TGTTCAGAGC AGGATGCCTG CTCCAGCAAA AACGGCCAGC CGGCCAGGGA ATTCTGCCCC 
AGGGAGGTGA CCGTGGAACA CACCACAAAC ATCCTCGAGG ATCACTCTTT CACAGAGTTT 
ATGGGTGTGT CAGATGGGAA AGATTTTGAT GGTTTGACGG ATTCTTCTGC TGGAGAACTT 
TCAAGTAGGA GGAACCTACC AAATACAGCG GACAGTAGGA GTTTCTCACA TGGGCCACAG 
GACCCTGGCC AGCTGTGTTC CTCGGCAGAG AATGACCAGG GGAGCGGCAC TGCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_015469025.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324511.1 Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X1, mRNA.

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CloneID	OBa52954
Clone ID Related Accession (Same CDS sequence)	XM_015469025.1 , XM_010818547.2
Accession Version	XM_015469025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646615.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGAGC ATGTCTCCAT CTCAGGCACA TAATCCTGGC 
TGCATTGTGC CCTCGGGAAG CAATGGCAGC AGCATGCCAG TAGAACTCAA TAGCAAACGT 
GAGAAGAAGA TTATTCTGAA GTTAATCTAC CTTGAGGAAT TGCACTTACT TGGAGACTCT 
GAGGAGGAGG AAAATGATCT AGAAGGTCTC AGCCGGAAGC TGATAAGTTC ACAGCCTTAT 
GTACCTGTGG AGTTCGCTGA CTTCAGCGTT TACAATGCCA GCTTGGAGAA CAGAGAGTGG 
TTTTCTTCTA AAGGCGATCT GACGAACTCA AAGGTCCTAG AGAAGGAGGT GTCCCGCAGC 
CCCACCACCA GCAGCATCAC CAGCGGCTAC TTTTCCCACA GTGCCTCCAA CGCCACCCTG 
TCCGACATGG TGGTCCCTTC CAGTGACAGC TCAGATCAGT TGGCCCTTCC AACGAAGGAC 
ACAGATTCCA GCGAGCATCC CGGACCGTCC CTTGGGCAGG ATTTCAGACC ATCTTCAAAC 
AAAGAGTTGA CAGAACTAGA AAGAGGCTTG GGAAAGGATA AGATGACCGT GGTGCCACTC 
AAGGAAAACA GTGCCTTAGC CAAAGGGAGC CCATTGTCCC CCAGTATCCC GGAGAAAAAC 
TCCAGAACCC TCTGTAGGAC CAGCTCGTGT TCAGAGCAGG ATGCCTGCTC CAGCAAAAAC 
GGCCAGCCGG CCAGGGAATT CTGCCCCAGG GAGGTGACCG TGGAACACAC CACAAACATC 
CTCGAGGATC ACTCTTTCAC AGAGTTTATG GGTGTGTCAG ATGGGAAAGA TTTTGATGGT 
TTGACGGATT CTTCTGCTGG AGAACTTTCA AGTAGGAGGA ACCTACCAAA TACAGCGGAC 
AGTAGGAGTT TCTCACATGG GCCACAGGAC CCTGGCCAGC TGTGTTCCTC GGCAGAGAAT 
GACCAGGGGA GCGGCACTGC TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324511.1
CDS	31..5397
Translation	MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQESMSPSQAHNPGCIVPSGSNGSSMPVELNSKREKKIILKLIYLEELHLLGDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_015469025.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469025.1

ORF Insert Sequence:

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGAGC ATGTCTCCAT CTCAGGCACA TAATCCTGGC 
TGCATTGTGC CCTCGGGAAG CAATGGCAGC AGCATGCCAG TAGAACTCAA TAGCAAACGT 
GAGAAGAAGA TTATTCTGAA GTTAATCTAC CTTGAGGAAT TGCACTTACT TGGAGACTCT 
GAGGAGGAGG AAAATGATCT AGAAGGTCTC AGCCGGAAGC TGATAAGTTC ACAGCCTTAT 
GTACCTGTGG AGTTCGCTGA CTTCAGCGTT TACAATGCCA GCTTGGAGAA CAGAGAGTGG 
TTTTCTTCTA AAGGCGATCT GACGAACTCA AAGGTCCTAG AGAAGGAGGT GTCCCGCAGC 
CCCACCACCA GCAGCATCAC CAGCGGCTAC TTTTCCCACA GTGCCTCCAA CGCCACCCTG 
TCCGACATGG TGGTCCCTTC CAGTGACAGC TCAGATCAGT TGGCCCTTCC AACGAAGGAC 
ACAGATTCCA GCGAGCATCC CGGACCGTCC CTTGGGCAGG ATTTCAGACC ATCTTCAAAC 
AAAGAGTTGA CAGAACTAGA AAGAGGCTTG GGAAAGGATA AGATGACCGT GGTGCCACTC 
AAGGAAAACA GTGCCTTAGC CAAAGGGAGC CCATTGTCCC CCAGTATCCC GGAGAAAAAC 
TCCAGAACCC TCTGTAGGAC CAGCTCGTGT TCAGAGCAGG ATGCCTGCTC CAGCAAAAAC 
GGCCAGCCGG CCAGGGAATT CTGCCCCAGG GAGGTGACCG TGGAACACAC CACAAACATC 
CTCGAGGATC ACTCTTTCAC AGAGTTTATG GGTGTGTCAG ATGGGAAAGA TTTTGATGGT 
TTGACGGATT CTTCTGCTGG AGAACTTTCA AGTAGGAGGA ACCTACCAAA TACAGCGGAC 
AGTAGGAGTT TCTCACATGG GCCACAGGAC CCTGGCCAGC TGTGTTCCTC GGCAGAGAAT 
GACCAGGGGA GCGGCACTGC TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_010818547.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816849.1 Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X1, mRNA.

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CloneID	OBa52954
Clone ID Related Accession (Same CDS sequence)	XM_015469025.1 , XM_010818547.2
Accession Version	XM_010818547.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104561.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818547.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGAGC ATGTCTCCAT CTCAGGCACA TAATCCTGGC 
TGCATTGTGC CCTCGGGAAG CAATGGCAGC AGCATGCCAG TAGAACTCAA TAGCAAACGT 
GAGAAGAAGA TTATTCTGAA GTTAATCTAC CTTGAGGAAT TGCACTTACT TGGAGACTCT 
GAGGAGGAGG AAAATGATCT AGAAGGTCTC AGCCGGAAGC TGATAAGTTC ACAGCCTTAT 
GTACCTGTGG AGTTCGCTGA CTTCAGCGTT TACAATGCCA GCTTGGAGAA CAGAGAGTGG 
TTTTCTTCTA AAGGCGATCT GACGAACTCA AAGGTCCTAG AGAAGGAGGT GTCCCGCAGC 
CCCACCACCA GCAGCATCAC CAGCGGCTAC TTTTCCCACA GTGCCTCCAA CGCCACCCTG 
TCCGACATGG TGGTCCCTTC CAGTGACAGC TCAGATCAGT TGGCCCTTCC AACGAAGGAC 
ACAGATTCCA GCGAGCATCC CGGACCGTCC CTTGGGCAGG ATTTCAGACC ATCTTCAAAC 
AAAGAGTTGA CAGAACTAGA AAGAGGCTTG GGAAAGGATA AGATGACCGT GGTGCCACTC 
AAGGAAAACA GTGCCTTAGC CAAAGGGAGC CCATTGTCCC CCAGTATCCC GGAGAAAAAC 
TCCAGAACCC TCTGTAGGAC CAGCTCGTGT TCAGAGCAGG ATGCCTGCTC CAGCAAAAAC 
GGCCAGCCGG CCAGGGAATT CTGCCCCAGG GAGGTGACCG TGGAACACAC CACAAACATC 
CTCGAGGATC ACTCTTTCAC AGAGTTTATG GGTGTGTCAG ATGGGAAAGA TTTTGATGGT 
TTGACGGATT CTTCTGCTGG AGAACTTTCA AGTAGGAGGA ACCTACCAAA TACAGCGGAC 
AGTAGGAGTT TCTCACATGG GCCACAGGAC CCTGGCCAGC TGTGTTCCTC GGCAGAGAAT 
GACCAGGGGA GCGGCACTGC TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816849.1
CDS	31..5397
Translation	MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQESMSPSQAHNPGCIVPSGSNGSSMPVELNSKREKKIILKLIYLEELHLLGDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_010818547.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818547.2

ORF Insert Sequence:

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGAGC ATGTCTCCAT CTCAGGCACA TAATCCTGGC 
TGCATTGTGC CCTCGGGAAG CAATGGCAGC AGCATGCCAG TAGAACTCAA TAGCAAACGT 
GAGAAGAAGA TTATTCTGAA GTTAATCTAC CTTGAGGAAT TGCACTTACT TGGAGACTCT 
GAGGAGGAGG AAAATGATCT AGAAGGTCTC AGCCGGAAGC TGATAAGTTC ACAGCCTTAT 
GTACCTGTGG AGTTCGCTGA CTTCAGCGTT TACAATGCCA GCTTGGAGAA CAGAGAGTGG 
TTTTCTTCTA AAGGCGATCT GACGAACTCA AAGGTCCTAG AGAAGGAGGT GTCCCGCAGC 
CCCACCACCA GCAGCATCAC CAGCGGCTAC TTTTCCCACA GTGCCTCCAA CGCCACCCTG 
TCCGACATGG TGGTCCCTTC CAGTGACAGC TCAGATCAGT TGGCCCTTCC AACGAAGGAC 
ACAGATTCCA GCGAGCATCC CGGACCGTCC CTTGGGCAGG ATTTCAGACC ATCTTCAAAC 
AAAGAGTTGA CAGAACTAGA AAGAGGCTTG GGAAAGGATA AGATGACCGT GGTGCCACTC 
AAGGAAAACA GTGCCTTAGC CAAAGGGAGC CCATTGTCCC CCAGTATCCC GGAGAAAAAC 
TCCAGAACCC TCTGTAGGAC CAGCTCGTGT TCAGAGCAGG ATGCCTGCTC CAGCAAAAAC 
GGCCAGCCGG CCAGGGAATT CTGCCCCAGG GAGGTGACCG TGGAACACAC CACAAACATC 
CTCGAGGATC ACTCTTTCAC AGAGTTTATG GGTGTGTCAG ATGGGAAAGA TTTTGATGGT 
TTGACGGATT CTTCTGCTGG AGAACTTTCA AGTAGGAGGA ACCTACCAAA TACAGCGGAC 
AGTAGGAGTT TCTCACATGG GCCACAGGAC CCTGGCCAGC TGTGTTCCTC GGCAGAGAAT 
GACCAGGGGA GCGGCACTGC TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_010818548.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816850.1 Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X2, mRNA.

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CloneID	OBa52955
Clone ID Related Accession (Same CDS sequence)	XM_010818548.2 , XM_015469026.1
Accession Version	XM_010818548.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104561.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818548.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGGAAGAAG ACTTAAACTG CGTGAGAGAG 
AGGTGGTCGG ATGCCCTCAT TAAACGGCGA GAATACCTAG ACGAACAGAT TAAAAAAGTC 
AGCAACAAAA AAGAGAAGAC GGAGGATGAC GTGGAGCGGG AAGCCCGGCT GGTGGAGCAG 
TGGGTGGGGC TGACGGAGGA GAGGAACGCT GTGCTGGTGC CTGCGCCGGG CAGCGGGATC 
CCCGGGGCGC CTGCCGACTG GATCCCACCA CCTGGAATGG AAACCCACAT ACCGGTTCTC 
TTCCTTGATT TGAACGCGGA TGACCTCAGT GCCAATGAGC AGCTCGTGGG CCCCCACGCA 
TCAGGCGTGA ACTCCATCCT CCCCAAGGAG CACGGCAGCC AGTTTTTCTA CCTGCCCATC 
ATAAAGCACA GTGACGAGGA GGTTTCAGCC ACAGCCTCCT GGGACTCCTC GGTGCATGAT 
TCCGTTCACT TGAACAGGGT CACCCCGCAG AATGAGAGGA TCTACCTGAT AGTGAAGACC 
ACAGTCCAGC TCAGCCACCC GGCCGCTATG GAGCTGGTCT TACGAAAACG GATTGCAGCC 
AACATTTACA ACAAACAGAG TTTCACCCAG AGTTTGAAGA GGAGAATATC ACTGAAGAAT 
ATATTTTATT CCTGTGGTGT AACCTATGAA ATAGTATCCA ACATACCAAA GGCAACAGAG 
GAGATTGAGG ACCGGGAGAC GCTGGCTCTC ATGGCTGCAA GGAGTGAGAA TGAAGGCACG 
TCGGATGGGG AGACGTACAT CGAGAAGTAC ACGCGGGGCG TGCTGCAGGT GGAGAACATT 
CTGAGCCTGG AACGACTCCG GCAGGCGGTG ACGGTCAAAG AAGCACTTTC CACCAAAGCT 
CGGCACCTAC GGCGAAGCCT CAGCACGCCC AACGTTCACA ATGTCTCTTC CAGTCGACCA 
GACCTTTCTG GCTTTGACGA TGATGATAAG GGTTGGCCAG AGAACCAGTT AGACATGTCT 
GACTACAGCC CGAGTTACCA GGATGTATCG TGTTATGGAA CTTTGCCTAG GGATTCACCT 
CGAAGGAGTA AAGAAGGTTG TACATCAGAG AATCCTCATG CCTTAACAGT CAGCCCTTTC 
AAAGCATTCT CTCCTCAGCC ACCAAAGTTT TTCAAGCCCC TAATGCCTGT AAAAGAGGAG 
CATAAGAAAA GGATAGCGCT TGAAGCAAGG CCTCTTCTAA GCCAGGAGAG CATGTCTCCA 
TCTCAGGCAC ATAATCCTGG CTGCATTGTG CCCTCGGGAA GCAATGGCAG CAGCATGCCA 
GTAGAACTCA ATAGCAAACG TGAGAAGAAG ATTATTCTGA AGTTAATCTA CCTTGAGGAA 
TTGCACTTAC TTGGAGACTC TGAGGAGGAG GAAAATGATC TAGAAGGTCT CAGCCGGAAG 
CTGATAAGTT CACAGCCTTA TGTACCTGTG GAGTTCGCTG ACTTCAGCGT TTACAATGCC 
AGCTTGGAGA ACAGAGAGTG GTTTTCTTCT AAAGGCGATC TGACGAACTC AAAGGTCCTA 
GAGAAGGAGG TGTCCCGCAG CCCCACCACC AGCAGCATCA CCAGCGGCTA CTTTTCCCAC 
AGTGCCTCCA ACGCCACCCT GTCCGACATG GTGGTCCCTT CCAGTGACAG CTCAGATCAG 
TTGGCCCTTC CAACGAAGGA CACAGATTCC AGCGAGCATC CCGGACCGTC CCTTGGGCAG 
GATTTCAGAC CATCTTCAAA CAAAGAGTTG ACAGAACTAG AAAGAGGCTT GGGAAAGGAT 
AAGATGACCG TGGTGCCACT CAAGGAAAAC AGTGCCTTAG CCAAAGGGAG CCCATTGTCC 
CCCAGTATCC CGGAGAAAAA CTCCAGAACC CTCTGTAGGA CCAGCTCGTG TTCAGAGCAG 
GATGCCTGCT CCAGCAAAAA CGGCCAGCCG GCCAGGGAAT TCTGCCCCAG GGAGGTGACC 
GTGGAACACA CCACAAACAT CCTCGAGGAT CACTCTTTCA CAGAGTTTAT GGGTGTGTCA 
GATGGGAAAG ATTTTGATGG TTTGACGGAT TCTTCTGCTG GAGAACTTTC AAGTAGGAGG 
AACCTACCAA ATACAGCGGA CAGTAGGAGT TTCTCACATG GGCCACAGGA CCCTGGCCAG 
CTGTGTTCCT CGGCAGAGAA TGACCAGGGG AGCGGCACTG CTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816850.1
CDS	31..5358
Translation	MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQESMSPSQAHNPGCIVPSGSNGSSMPVELNSKREKKIILKLIYLEELHLLGDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_010818548.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818548.2

ORF Insert Sequence:

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGGAAGAAG ACTTAAACTG CGTGAGAGAG 
AGGTGGTCGG ATGCCCTCAT TAAACGGCGA GAATACCTAG ACGAACAGAT TAAAAAAGTC 
AGCAACAAAA AAGAGAAGAC GGAGGATGAC GTGGAGCGGG AAGCCCGGCT GGTGGAGCAG 
TGGGTGGGGC TGACGGAGGA GAGGAACGCT GTGCTGGTGC CTGCGCCGGG CAGCGGGATC 
CCCGGGGCGC CTGCCGACTG GATCCCACCA CCTGGAATGG AAACCCACAT ACCGGTTCTC 
TTCCTTGATT TGAACGCGGA TGACCTCAGT GCCAATGAGC AGCTCGTGGG CCCCCACGCA 
TCAGGCGTGA ACTCCATCCT CCCCAAGGAG CACGGCAGCC AGTTTTTCTA CCTGCCCATC 
ATAAAGCACA GTGACGAGGA GGTTTCAGCC ACAGCCTCCT GGGACTCCTC GGTGCATGAT 
TCCGTTCACT TGAACAGGGT CACCCCGCAG AATGAGAGGA TCTACCTGAT AGTGAAGACC 
ACAGTCCAGC TCAGCCACCC GGCCGCTATG GAGCTGGTCT TACGAAAACG GATTGCAGCC 
AACATTTACA ACAAACAGAG TTTCACCCAG AGTTTGAAGA GGAGAATATC ACTGAAGAAT 
ATATTTTATT CCTGTGGTGT AACCTATGAA ATAGTATCCA ACATACCAAA GGCAACAGAG 
GAGATTGAGG ACCGGGAGAC GCTGGCTCTC ATGGCTGCAA GGAGTGAGAA TGAAGGCACG 
TCGGATGGGG AGACGTACAT CGAGAAGTAC ACGCGGGGCG TGCTGCAGGT GGAGAACATT 
CTGAGCCTGG AACGACTCCG GCAGGCGGTG ACGGTCAAAG AAGCACTTTC CACCAAAGCT 
CGGCACCTAC GGCGAAGCCT CAGCACGCCC AACGTTCACA ATGTCTCTTC CAGTCGACCA 
GACCTTTCTG GCTTTGACGA TGATGATAAG GGTTGGCCAG AGAACCAGTT AGACATGTCT 
GACTACAGCC CGAGTTACCA GGATGTATCG TGTTATGGAA CTTTGCCTAG GGATTCACCT 
CGAAGGAGTA AAGAAGGTTG TACATCAGAG AATCCTCATG CCTTAACAGT CAGCCCTTTC 
AAAGCATTCT CTCCTCAGCC ACCAAAGTTT TTCAAGCCCC TAATGCCTGT AAAAGAGGAG 
CATAAGAAAA GGATAGCGCT TGAAGCAAGG CCTCTTCTAA GCCAGGAGAG CATGTCTCCA 
TCTCAGGCAC ATAATCCTGG CTGCATTGTG CCCTCGGGAA GCAATGGCAG CAGCATGCCA 
GTAGAACTCA ATAGCAAACG TGAGAAGAAG ATTATTCTGA AGTTAATCTA CCTTGAGGAA 
TTGCACTTAC TTGGAGACTC TGAGGAGGAG GAAAATGATC TAGAAGGTCT CAGCCGGAAG 
CTGATAAGTT CACAGCCTTA TGTACCTGTG GAGTTCGCTG ACTTCAGCGT TTACAATGCC 
AGCTTGGAGA ACAGAGAGTG GTTTTCTTCT AAAGGCGATC TGACGAACTC AAAGGTCCTA 
GAGAAGGAGG TGTCCCGCAG CCCCACCACC AGCAGCATCA CCAGCGGCTA CTTTTCCCAC 
AGTGCCTCCA ACGCCACCCT GTCCGACATG GTGGTCCCTT CCAGTGACAG CTCAGATCAG 
TTGGCCCTTC CAACGAAGGA CACAGATTCC AGCGAGCATC CCGGACCGTC CCTTGGGCAG 
GATTTCAGAC CATCTTCAAA CAAAGAGTTG ACAGAACTAG AAAGAGGCTT GGGAAAGGAT 
AAGATGACCG TGGTGCCACT CAAGGAAAAC AGTGCCTTAG CCAAAGGGAG CCCATTGTCC 
CCCAGTATCC CGGAGAAAAA CTCCAGAACC CTCTGTAGGA CCAGCTCGTG TTCAGAGCAG 
GATGCCTGCT CCAGCAAAAA CGGCCAGCCG GCCAGGGAAT TCTGCCCCAG GGAGGTGACC 
GTGGAACACA CCACAAACAT CCTCGAGGAT CACTCTTTCA CAGAGTTTAT GGGTGTGTCA 
GATGGGAAAG ATTTTGATGG TTTGACGGAT TCTTCTGCTG GAGAACTTTC AAGTAGGAGG 
AACCTACCAA ATACAGCGGA CAGTAGGAGT TTCTCACATG GGCCACAGGA CCCTGGCCAG 
CTGTGTTCCT CGGCAGAGAA TGACCAGGGG AGCGGCACTG CTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_015469026.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324512.1 Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X2, mRNA.

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CloneID	OBa52955
Clone ID Related Accession (Same CDS sequence)	XM_010818548.2 , XM_015469026.1
Accession Version	XM_015469026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646615.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGGAAGAAG ACTTAAACTG CGTGAGAGAG 
AGGTGGTCGG ATGCCCTCAT TAAACGGCGA GAATACCTAG ACGAACAGAT TAAAAAAGTC 
AGCAACAAAA AAGAGAAGAC GGAGGATGAC GTGGAGCGGG AAGCCCGGCT GGTGGAGCAG 
TGGGTGGGGC TGACGGAGGA GAGGAACGCT GTGCTGGTGC CTGCGCCGGG CAGCGGGATC 
CCCGGGGCGC CTGCCGACTG GATCCCACCA CCTGGAATGG AAACCCACAT ACCGGTTCTC 
TTCCTTGATT TGAACGCGGA TGACCTCAGT GCCAATGAGC AGCTCGTGGG CCCCCACGCA 
TCAGGCGTGA ACTCCATCCT CCCCAAGGAG CACGGCAGCC AGTTTTTCTA CCTGCCCATC 
ATAAAGCACA GTGACGAGGA GGTTTCAGCC ACAGCCTCCT GGGACTCCTC GGTGCATGAT 
TCCGTTCACT TGAACAGGGT CACCCCGCAG AATGAGAGGA TCTACCTGAT AGTGAAGACC 
ACAGTCCAGC TCAGCCACCC GGCCGCTATG GAGCTGGTCT TACGAAAACG GATTGCAGCC 
AACATTTACA ACAAACAGAG TTTCACCCAG AGTTTGAAGA GGAGAATATC ACTGAAGAAT 
ATATTTTATT CCTGTGGTGT AACCTATGAA ATAGTATCCA ACATACCAAA GGCAACAGAG 
GAGATTGAGG ACCGGGAGAC GCTGGCTCTC ATGGCTGCAA GGAGTGAGAA TGAAGGCACG 
TCGGATGGGG AGACGTACAT CGAGAAGTAC ACGCGGGGCG TGCTGCAGGT GGAGAACATT 
CTGAGCCTGG AACGACTCCG GCAGGCGGTG ACGGTCAAAG AAGCACTTTC CACCAAAGCT 
CGGCACCTAC GGCGAAGCCT CAGCACGCCC AACGTTCACA ATGTCTCTTC CAGTCGACCA 
GACCTTTCTG GCTTTGACGA TGATGATAAG GGTTGGCCAG AGAACCAGTT AGACATGTCT 
GACTACAGCC CGAGTTACCA GGATGTATCG TGTTATGGAA CTTTGCCTAG GGATTCACCT 
CGAAGGAGTA AAGAAGGTTG TACATCAGAG AATCCTCATG CCTTAACAGT CAGCCCTTTC 
AAAGCATTCT CTCCTCAGCC ACCAAAGTTT TTCAAGCCCC TAATGCCTGT AAAAGAGGAG 
CATAAGAAAA GGATAGCGCT TGAAGCAAGG CCTCTTCTAA GCCAGGAGAG CATGTCTCCA 
TCTCAGGCAC ATAATCCTGG CTGCATTGTG CCCTCGGGAA GCAATGGCAG CAGCATGCCA 
GTAGAACTCA ATAGCAAACG TGAGAAGAAG ATTATTCTGA AGTTAATCTA CCTTGAGGAA 
TTGCACTTAC TTGGAGACTC TGAGGAGGAG GAAAATGATC TAGAAGGTCT CAGCCGGAAG 
CTGATAAGTT CACAGCCTTA TGTACCTGTG GAGTTCGCTG ACTTCAGCGT TTACAATGCC 
AGCTTGGAGA ACAGAGAGTG GTTTTCTTCT AAAGGCGATC TGACGAACTC AAAGGTCCTA 
GAGAAGGAGG TGTCCCGCAG CCCCACCACC AGCAGCATCA CCAGCGGCTA CTTTTCCCAC 
AGTGCCTCCA ACGCCACCCT GTCCGACATG GTGGTCCCTT CCAGTGACAG CTCAGATCAG 
TTGGCCCTTC CAACGAAGGA CACAGATTCC AGCGAGCATC CCGGACCGTC CCTTGGGCAG 
GATTTCAGAC CATCTTCAAA CAAAGAGTTG ACAGAACTAG AAAGAGGCTT GGGAAAGGAT 
AAGATGACCG TGGTGCCACT CAAGGAAAAC AGTGCCTTAG CCAAAGGGAG CCCATTGTCC 
CCCAGTATCC CGGAGAAAAA CTCCAGAACC CTCTGTAGGA CCAGCTCGTG TTCAGAGCAG 
GATGCCTGCT CCAGCAAAAA CGGCCAGCCG GCCAGGGAAT TCTGCCCCAG GGAGGTGACC 
GTGGAACACA CCACAAACAT CCTCGAGGAT CACTCTTTCA CAGAGTTTAT GGGTGTGTCA 
GATGGGAAAG ATTTTGATGG TTTGACGGAT TCTTCTGCTG GAGAACTTTC AAGTAGGAGG 
AACCTACCAA ATACAGCGGA CAGTAGGAGT TTCTCACATG GGCCACAGGA CCCTGGCCAG 
CTGTGTTCCT CGGCAGAGAA TGACCAGGGG AGCGGCACTG CTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324512.1
CDS	31..5358
Translation	MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQESMSPSQAHNPGCIVPSGSNGSSMPVELNSKREKKIILKLIYLEELHLLGDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_015469026.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469026.1

ORF Insert Sequence:

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGGAAGAAG ACTTAAACTG CGTGAGAGAG 
AGGTGGTCGG ATGCCCTCAT TAAACGGCGA GAATACCTAG ACGAACAGAT TAAAAAAGTC 
AGCAACAAAA AAGAGAAGAC GGAGGATGAC GTGGAGCGGG AAGCCCGGCT GGTGGAGCAG 
TGGGTGGGGC TGACGGAGGA GAGGAACGCT GTGCTGGTGC CTGCGCCGGG CAGCGGGATC 
CCCGGGGCGC CTGCCGACTG GATCCCACCA CCTGGAATGG AAACCCACAT ACCGGTTCTC 
TTCCTTGATT TGAACGCGGA TGACCTCAGT GCCAATGAGC AGCTCGTGGG CCCCCACGCA 
TCAGGCGTGA ACTCCATCCT CCCCAAGGAG CACGGCAGCC AGTTTTTCTA CCTGCCCATC 
ATAAAGCACA GTGACGAGGA GGTTTCAGCC ACAGCCTCCT GGGACTCCTC GGTGCATGAT 
TCCGTTCACT TGAACAGGGT CACCCCGCAG AATGAGAGGA TCTACCTGAT AGTGAAGACC 
ACAGTCCAGC TCAGCCACCC GGCCGCTATG GAGCTGGTCT TACGAAAACG GATTGCAGCC 
AACATTTACA ACAAACAGAG TTTCACCCAG AGTTTGAAGA GGAGAATATC ACTGAAGAAT 
ATATTTTATT CCTGTGGTGT AACCTATGAA ATAGTATCCA ACATACCAAA GGCAACAGAG 
GAGATTGAGG ACCGGGAGAC GCTGGCTCTC ATGGCTGCAA GGAGTGAGAA TGAAGGCACG 
TCGGATGGGG AGACGTACAT CGAGAAGTAC ACGCGGGGCG TGCTGCAGGT GGAGAACATT 
CTGAGCCTGG AACGACTCCG GCAGGCGGTG ACGGTCAAAG AAGCACTTTC CACCAAAGCT 
CGGCACCTAC GGCGAAGCCT CAGCACGCCC AACGTTCACA ATGTCTCTTC CAGTCGACCA 
GACCTTTCTG GCTTTGACGA TGATGATAAG GGTTGGCCAG AGAACCAGTT AGACATGTCT 
GACTACAGCC CGAGTTACCA GGATGTATCG TGTTATGGAA CTTTGCCTAG GGATTCACCT 
CGAAGGAGTA AAGAAGGTTG TACATCAGAG AATCCTCATG CCTTAACAGT CAGCCCTTTC 
AAAGCATTCT CTCCTCAGCC ACCAAAGTTT TTCAAGCCCC TAATGCCTGT AAAAGAGGAG 
CATAAGAAAA GGATAGCGCT TGAAGCAAGG CCTCTTCTAA GCCAGGAGAG CATGTCTCCA 
TCTCAGGCAC ATAATCCTGG CTGCATTGTG CCCTCGGGAA GCAATGGCAG CAGCATGCCA 
GTAGAACTCA ATAGCAAACG TGAGAAGAAG ATTATTCTGA AGTTAATCTA CCTTGAGGAA 
TTGCACTTAC TTGGAGACTC TGAGGAGGAG GAAAATGATC TAGAAGGTCT CAGCCGGAAG 
CTGATAAGTT CACAGCCTTA TGTACCTGTG GAGTTCGCTG ACTTCAGCGT TTACAATGCC 
AGCTTGGAGA ACAGAGAGTG GTTTTCTTCT AAAGGCGATC TGACGAACTC AAAGGTCCTA 
GAGAAGGAGG TGTCCCGCAG CCCCACCACC AGCAGCATCA CCAGCGGCTA CTTTTCCCAC 
AGTGCCTCCA ACGCCACCCT GTCCGACATG GTGGTCCCTT CCAGTGACAG CTCAGATCAG 
TTGGCCCTTC CAACGAAGGA CACAGATTCC AGCGAGCATC CCGGACCGTC CCTTGGGCAG 
GATTTCAGAC CATCTTCAAA CAAAGAGTTG ACAGAACTAG AAAGAGGCTT GGGAAAGGAT 
AAGATGACCG TGGTGCCACT CAAGGAAAAC AGTGCCTTAG CCAAAGGGAG CCCATTGTCC 
CCCAGTATCC CGGAGAAAAA CTCCAGAACC CTCTGTAGGA CCAGCTCGTG TTCAGAGCAG 
GATGCCTGCT CCAGCAAAAA CGGCCAGCCG GCCAGGGAAT TCTGCCCCAG GGAGGTGACC 
GTGGAACACA CCACAAACAT CCTCGAGGAT CACTCTTTCA CAGAGTTTAT GGGTGTGTCA 
GATGGGAAAG ATTTTGATGG TTTGACGGAT TCTTCTGCTG GAGAACTTTC AAGTAGGAGG 
AACCTACCAA ATACAGCGGA CAGTAGGAGT TTCTCACATG GGCCACAGGA CCCTGGCCAG 
CTGTGTTCCT CGGCAGAGAA TGACCAGGGG AGCGGCACTG CTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_010818549.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816851.1 Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X3, mRNA.

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CloneID	OBa52956
Clone ID Related Accession (Same CDS sequence)	XM_010818549.2 , XM_015469027.1
Accession Version	XM_010818549.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104561.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818549.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGAGC ATGTCTCCAT CTCAGGCACA TAATCCTGGC 
TGCATTGTGC CCTCGGGAAG CAATGGCAGC AGCATGCCAG TAGAACTCAA TAGCAAACGT 
GAGAAGAAGA TTGACTCTGA GGAGGAGGAA AATGATCTAG AAGGTCTCAG CCGGAAGCTG 
ATAAGTTCAC AGCCTTATGT ACCTGTGGAG TTCGCTGACT TCAGCGTTTA CAATGCCAGC 
TTGGAGAACA GAGAGTGGTT TTCTTCTAAA GGCGATCTGA CGAACTCAAA GGTCCTAGAG 
AAGGAGGTGT CCCGCAGCCC CACCACCAGC AGCATCACCA GCGGCTACTT TTCCCACAGT 
GCCTCCAACG CCACCCTGTC CGACATGGTG GTCCCTTCCA GTGACAGCTC AGATCAGTTG 
GCCCTTCCAA CGAAGGACAC AGATTCCAGC GAGCATCCCG GACCGTCCCT TGGGCAGGAT 
TTCAGACCAT CTTCAAACAA AGAGTTGACA GAACTAGAAA GAGGCTTGGG AAAGGATAAG 
ATGACCGTGG TGCCACTCAA GGAAAACAGT GCCTTAGCCA AAGGGAGCCC ATTGTCCCCC 
AGTATCCCGG AGAAAAACTC CAGAACCCTC TGTAGGACCA GCTCGTGTTC AGAGCAGGAT 
GCCTGCTCCA GCAAAAACGG CCAGCCGGCC AGGGAATTCT GCCCCAGGGA GGTGACCGTG 
GAACACACCA CAAACATCCT CGAGGATCAC TCTTTCACAG AGTTTATGGG TGTGTCAGAT 
GGGAAAGATT TTGATGGTTT GACGGATTCT TCTGCTGGAG AACTTTCAAG TAGGAGGAAC 
CTACCAAATA CAGCGGACAG TAGGAGTTTC TCACATGGGC CACAGGACCC TGGCCAGCTG 
TGTTCCTCGG CAGAGAATGA CCAGGGGAGC GGCACTGCTC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816851.1
CDS	31..5355
Translation	MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQESMSPSQAHNPGCIVPSGSNGSSMPVELNSKREKKIDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_010818549.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818549.2

ORF Insert Sequence:

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGAGC ATGTCTCCAT CTCAGGCACA TAATCCTGGC 
TGCATTGTGC CCTCGGGAAG CAATGGCAGC AGCATGCCAG TAGAACTCAA TAGCAAACGT 
GAGAAGAAGA TTGACTCTGA GGAGGAGGAA AATGATCTAG AAGGTCTCAG CCGGAAGCTG 
ATAAGTTCAC AGCCTTATGT ACCTGTGGAG TTCGCTGACT TCAGCGTTTA CAATGCCAGC 
TTGGAGAACA GAGAGTGGTT TTCTTCTAAA GGCGATCTGA CGAACTCAAA GGTCCTAGAG 
AAGGAGGTGT CCCGCAGCCC CACCACCAGC AGCATCACCA GCGGCTACTT TTCCCACAGT 
GCCTCCAACG CCACCCTGTC CGACATGGTG GTCCCTTCCA GTGACAGCTC AGATCAGTTG 
GCCCTTCCAA CGAAGGACAC AGATTCCAGC GAGCATCCCG GACCGTCCCT TGGGCAGGAT 
TTCAGACCAT CTTCAAACAA AGAGTTGACA GAACTAGAAA GAGGCTTGGG AAAGGATAAG 
ATGACCGTGG TGCCACTCAA GGAAAACAGT GCCTTAGCCA AAGGGAGCCC ATTGTCCCCC 
AGTATCCCGG AGAAAAACTC CAGAACCCTC TGTAGGACCA GCTCGTGTTC AGAGCAGGAT 
GCCTGCTCCA GCAAAAACGG CCAGCCGGCC AGGGAATTCT GCCCCAGGGA GGTGACCGTG 
GAACACACCA CAAACATCCT CGAGGATCAC TCTTTCACAG AGTTTATGGG TGTGTCAGAT 
GGGAAAGATT TTGATGGTTT GACGGATTCT TCTGCTGGAG AACTTTCAAG TAGGAGGAAC 
CTACCAAATA CAGCGGACAG TAGGAGTTTC TCACATGGGC CACAGGACCC TGGCCAGCTG 
TGTTCCTCGG CAGAGAATGA CCAGGGGAGC GGCACTGCTC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_015469027.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324513.1 Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X3, mRNA.

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CloneID	OBa52956
Clone ID Related Accession (Same CDS sequence)	XM_010818549.2 , XM_015469027.1
Accession Version	XM_015469027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646615.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGAGC ATGTCTCCAT CTCAGGCACA TAATCCTGGC 
TGCATTGTGC CCTCGGGAAG CAATGGCAGC AGCATGCCAG TAGAACTCAA TAGCAAACGT 
GAGAAGAAGA TTGACTCTGA GGAGGAGGAA AATGATCTAG AAGGTCTCAG CCGGAAGCTG 
ATAAGTTCAC AGCCTTATGT ACCTGTGGAG TTCGCTGACT TCAGCGTTTA CAATGCCAGC 
TTGGAGAACA GAGAGTGGTT TTCTTCTAAA GGCGATCTGA CGAACTCAAA GGTCCTAGAG 
AAGGAGGTGT CCCGCAGCCC CACCACCAGC AGCATCACCA GCGGCTACTT TTCCCACAGT 
GCCTCCAACG CCACCCTGTC CGACATGGTG GTCCCTTCCA GTGACAGCTC AGATCAGTTG 
GCCCTTCCAA CGAAGGACAC AGATTCCAGC GAGCATCCCG GACCGTCCCT TGGGCAGGAT 
TTCAGACCAT CTTCAAACAA AGAGTTGACA GAACTAGAAA GAGGCTTGGG AAAGGATAAG 
ATGACCGTGG TGCCACTCAA GGAAAACAGT GCCTTAGCCA AAGGGAGCCC ATTGTCCCCC 
AGTATCCCGG AGAAAAACTC CAGAACCCTC TGTAGGACCA GCTCGTGTTC AGAGCAGGAT 
GCCTGCTCCA GCAAAAACGG CCAGCCGGCC AGGGAATTCT GCCCCAGGGA GGTGACCGTG 
GAACACACCA CAAACATCCT CGAGGATCAC TCTTTCACAG AGTTTATGGG TGTGTCAGAT 
GGGAAAGATT TTGATGGTTT GACGGATTCT TCTGCTGGAG AACTTTCAAG TAGGAGGAAC 
CTACCAAATA CAGCGGACAG TAGGAGTTTC TCACATGGGC CACAGGACCC TGGCCAGCTG 
TGTTCCTCGG CAGAGAATGA CCAGGGGAGC GGCACTGCTC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324513.1
CDS	31..5355
Translation	MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQESMSPSQAHNPGCIVPSGSNGSSMPVELNSKREKKIDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_015469027.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469027.1

ORF Insert Sequence:

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGAGC ATGTCTCCAT CTCAGGCACA TAATCCTGGC 
TGCATTGTGC CCTCGGGAAG CAATGGCAGC AGCATGCCAG TAGAACTCAA TAGCAAACGT 
GAGAAGAAGA TTGACTCTGA GGAGGAGGAA AATGATCTAG AAGGTCTCAG CCGGAAGCTG 
ATAAGTTCAC AGCCTTATGT ACCTGTGGAG TTCGCTGACT TCAGCGTTTA CAATGCCAGC 
TTGGAGAACA GAGAGTGGTT TTCTTCTAAA GGCGATCTGA CGAACTCAAA GGTCCTAGAG 
AAGGAGGTGT CCCGCAGCCC CACCACCAGC AGCATCACCA GCGGCTACTT TTCCCACAGT 
GCCTCCAACG CCACCCTGTC CGACATGGTG GTCCCTTCCA GTGACAGCTC AGATCAGTTG 
GCCCTTCCAA CGAAGGACAC AGATTCCAGC GAGCATCCCG GACCGTCCCT TGGGCAGGAT 
TTCAGACCAT CTTCAAACAA AGAGTTGACA GAACTAGAAA GAGGCTTGGG AAAGGATAAG 
ATGACCGTGG TGCCACTCAA GGAAAACAGT GCCTTAGCCA AAGGGAGCCC ATTGTCCCCC 
AGTATCCCGG AGAAAAACTC CAGAACCCTC TGTAGGACCA GCTCGTGTTC AGAGCAGGAT 
GCCTGCTCCA GCAAAAACGG CCAGCCGGCC AGGGAATTCT GCCCCAGGGA GGTGACCGTG 
GAACACACCA CAAACATCCT CGAGGATCAC TCTTTCACAG AGTTTATGGG TGTGTCAGAT 
GGGAAAGATT TTGATGGTTT GACGGATTCT TCTGCTGGAG AACTTTCAAG TAGGAGGAAC 
CTACCAAATA CAGCGGACAG TAGGAGTTTC TCACATGGGC CACAGGACCC TGGCCAGCTG 
TGTTCCTCGG CAGAGAATGA CCAGGGGAGC GGCACTGCTC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_015469029.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324515.1 Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X5, mRNA.

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CloneID	OBa52957
Clone ID Related Accession (Same CDS sequence)	XM_015469029.1 , XM_010818551.2
Accession Version	XM_015469029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646615.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGAGC ATGTCTCCAT CTCAGGCACA TAATCCTGGC 
TGCATTGTGC CCTCGGGAAG CAATGGCAGC AGCATGCCAG TAGAACTCAA TAGCAAACGT 
GAGAAGAAGA TTATTCTGAA GTTAATCTAC CTTGAGGAAT TGCACTTACT TGGAGACTCT 
GAGGAGGAGG AAAATGATCT AGAAGGTCTC AGCCGGAAGC TGATAAGTTC ACAGCCTTAT 
GTACCTGTGG AGTTCGCTGA CTTCAGCGTT TACAATGCCA GCTTGGAGAA CAGAGAGTGG 
TTTTCTTCTA AAGGCGATCT GACGAACTCA AAGGTCCTAG AGAAGGAGGT GTCCCGCAGC 
CCCACCACCA GCAGCATCAC CAGCGGCTAC TTTTCCCACA GTGCCTCCAA CGCCACCCTG 
TCCGACATGG TGGTCCCTTC CAGTGACAGC TCAGATCAGT TGGCCCTTCC AACGAAGGAC 
ACAGATTCCA GCGAGCATCC CGGACCGTCC CTTGGGCAGG ATTTCAGACC ATCTTCAAAC 
AAAGAGTTGA CAGAACTAGA AAGAGGCTTG GGAAAGGATA AGATGACCGT GGTGCCACTC 
AAGGAAAACA GTGCCTTAGC CAAAGGGAGC CCATTGTCCC CCAGTATCCC GGAGAAAAAC 
TCCAGAACCC TCTGTAGGAC CAGCTCGTGT TCAGAGCAGG ATGCCTGCTC CAGCAAAAAC 
GGCCAGCCGG CCAGGGAATT CTGCCCCAGG GAGGTGACCG TGGAACACAC CACAAACATC 
CTCGAGGATC ACTCTTTCAC AGAGTTTATG GGTGTGTCAG ATGGGAAAGA TTTTGATGGT 
TTGACGGATT CTTCTGCTGG AGAACTTTCA AGTAGGAGGA ACCTACCAAA TACAGCGGAC 
AGTAGGAGTT TCTCACATGG GCCACAGGAC CCTGGCCAGC TGTGTTCCTC GGCAGAGAAT 
GACCAGGGGA GCGGCACTGC TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324515.1
CDS	31..5277
Translation	MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQESMSPSQAHNPGCIVPSGSNGSSMPVELNSKREKKIILKLIYLEELHLLGDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_015469029.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469029.1

ORF Insert Sequence:

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGAGC ATGTCTCCAT CTCAGGCACA TAATCCTGGC 
TGCATTGTGC CCTCGGGAAG CAATGGCAGC AGCATGCCAG TAGAACTCAA TAGCAAACGT 
GAGAAGAAGA TTATTCTGAA GTTAATCTAC CTTGAGGAAT TGCACTTACT TGGAGACTCT 
GAGGAGGAGG AAAATGATCT AGAAGGTCTC AGCCGGAAGC TGATAAGTTC ACAGCCTTAT 
GTACCTGTGG AGTTCGCTGA CTTCAGCGTT TACAATGCCA GCTTGGAGAA CAGAGAGTGG 
TTTTCTTCTA AAGGCGATCT GACGAACTCA AAGGTCCTAG AGAAGGAGGT GTCCCGCAGC 
CCCACCACCA GCAGCATCAC CAGCGGCTAC TTTTCCCACA GTGCCTCCAA CGCCACCCTG 
TCCGACATGG TGGTCCCTTC CAGTGACAGC TCAGATCAGT TGGCCCTTCC AACGAAGGAC 
ACAGATTCCA GCGAGCATCC CGGACCGTCC CTTGGGCAGG ATTTCAGACC ATCTTCAAAC 
AAAGAGTTGA CAGAACTAGA AAGAGGCTTG GGAAAGGATA AGATGACCGT GGTGCCACTC 
AAGGAAAACA GTGCCTTAGC CAAAGGGAGC CCATTGTCCC CCAGTATCCC GGAGAAAAAC 
TCCAGAACCC TCTGTAGGAC CAGCTCGTGT TCAGAGCAGG ATGCCTGCTC CAGCAAAAAC 
GGCCAGCCGG CCAGGGAATT CTGCCCCAGG GAGGTGACCG TGGAACACAC CACAAACATC 
CTCGAGGATC ACTCTTTCAC AGAGTTTATG GGTGTGTCAG ATGGGAAAGA TTTTGATGGT 
TTGACGGATT CTTCTGCTGG AGAACTTTCA AGTAGGAGGA ACCTACCAAA TACAGCGGAC 
AGTAGGAGTT TCTCACATGG GCCACAGGAC CCTGGCCAGC TGTGTTCCTC GGCAGAGAAT 
GACCAGGGGA GCGGCACTGC TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_010818551.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816853.1 Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X5, mRNA.

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CloneID	OBa52957
Clone ID Related Accession (Same CDS sequence)	XM_015469029.1 , XM_010818551.2
Accession Version	XM_010818551.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104561.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGAGC ATGTCTCCAT CTCAGGCACA TAATCCTGGC 
TGCATTGTGC CCTCGGGAAG CAATGGCAGC AGCATGCCAG TAGAACTCAA TAGCAAACGT 
GAGAAGAAGA TTATTCTGAA GTTAATCTAC CTTGAGGAAT TGCACTTACT TGGAGACTCT 
GAGGAGGAGG AAAATGATCT AGAAGGTCTC AGCCGGAAGC TGATAAGTTC ACAGCCTTAT 
GTACCTGTGG AGTTCGCTGA CTTCAGCGTT TACAATGCCA GCTTGGAGAA CAGAGAGTGG 
TTTTCTTCTA AAGGCGATCT GACGAACTCA AAGGTCCTAG AGAAGGAGGT GTCCCGCAGC 
CCCACCACCA GCAGCATCAC CAGCGGCTAC TTTTCCCACA GTGCCTCCAA CGCCACCCTG 
TCCGACATGG TGGTCCCTTC CAGTGACAGC TCAGATCAGT TGGCCCTTCC AACGAAGGAC 
ACAGATTCCA GCGAGCATCC CGGACCGTCC CTTGGGCAGG ATTTCAGACC ATCTTCAAAC 
AAAGAGTTGA CAGAACTAGA AAGAGGCTTG GGAAAGGATA AGATGACCGT GGTGCCACTC 
AAGGAAAACA GTGCCTTAGC CAAAGGGAGC CCATTGTCCC CCAGTATCCC GGAGAAAAAC 
TCCAGAACCC TCTGTAGGAC CAGCTCGTGT TCAGAGCAGG ATGCCTGCTC CAGCAAAAAC 
GGCCAGCCGG CCAGGGAATT CTGCCCCAGG GAGGTGACCG TGGAACACAC CACAAACATC 
CTCGAGGATC ACTCTTTCAC AGAGTTTATG GGTGTGTCAG ATGGGAAAGA TTTTGATGGT 
TTGACGGATT CTTCTGCTGG AGAACTTTCA AGTAGGAGGA ACCTACCAAA TACAGCGGAC 
AGTAGGAGTT TCTCACATGG GCCACAGGAC CCTGGCCAGC TGTGTTCCTC GGCAGAGAAT 
GACCAGGGGA GCGGCACTGC TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816853.1
CDS	31..5277
Translation	MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGCTSENPHALTVSPFKAFSPQPPKFFKPLMPVKEEHKKRIALEARPLLSQESMSPSQAHNPGCIVPSGSNGSSMPVELNSKREKKIILKLIYLEELHLLGDSEEEENDLEGLSRKLISSQPYVPVEFADFSVYNASLENREWFSSKGDLTNSKVLEKEVSRSPTTSSITSGYFSHSASNATLSDMVVPSSDSSDQLALPTKDTDSSEHPGPSLGQDFRPSSNKELTELERGLGKDKMTVVPLKENSALAKGSPLSPSIPEKNSRTLCRTSSCSEQDACSSKNGQPAREFCPREVTVEHTTNILEDHSFTEFMGVSDGKDFDGLTDSSAGELSSRRNLPNTADSRSFSHGPQDPGQLCSSAENDQGSGTAR

Target ORF information:

RefSeq Version	XM_010818551.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818551.2

ORF Insert Sequence:

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGTTGT 
ACATCAGAGA ATCCTCATGC CTTAACAGTC AGCCCTTTCA AAGCATTCTC TCCTCAGCCA 
CCAAAGTTTT TCAAGCCCCT AATGCCTGTA AAAGAGGAGC ATAAGAAAAG GATAGCGCTT 
GAAGCAAGGC CTCTTCTAAG CCAGGAGAGC ATGTCTCCAT CTCAGGCACA TAATCCTGGC 
TGCATTGTGC CCTCGGGAAG CAATGGCAGC AGCATGCCAG TAGAACTCAA TAGCAAACGT 
GAGAAGAAGA TTATTCTGAA GTTAATCTAC CTTGAGGAAT TGCACTTACT TGGAGACTCT 
GAGGAGGAGG AAAATGATCT AGAAGGTCTC AGCCGGAAGC TGATAAGTTC ACAGCCTTAT 
GTACCTGTGG AGTTCGCTGA CTTCAGCGTT TACAATGCCA GCTTGGAGAA CAGAGAGTGG 
TTTTCTTCTA AAGGCGATCT GACGAACTCA AAGGTCCTAG AGAAGGAGGT GTCCCGCAGC 
CCCACCACCA GCAGCATCAC CAGCGGCTAC TTTTCCCACA GTGCCTCCAA CGCCACCCTG 
TCCGACATGG TGGTCCCTTC CAGTGACAGC TCAGATCAGT TGGCCCTTCC AACGAAGGAC 
ACAGATTCCA GCGAGCATCC CGGACCGTCC CTTGGGCAGG ATTTCAGACC ATCTTCAAAC 
AAAGAGTTGA CAGAACTAGA AAGAGGCTTG GGAAAGGATA AGATGACCGT GGTGCCACTC 
AAGGAAAACA GTGCCTTAGC CAAAGGGAGC CCATTGTCCC CCAGTATCCC GGAGAAAAAC 
TCCAGAACCC TCTGTAGGAC CAGCTCGTGT TCAGAGCAGG ATGCCTGCTC CAGCAAAAAC 
GGCCAGCCGG CCAGGGAATT CTGCCCCAGG GAGGTGACCG TGGAACACAC CACAAACATC 
CTCGAGGATC ACTCTTTCAC AGAGTTTATG GGTGTGTCAG ATGGGAAAGA TTTTGATGGT 
TTGACGGATT CTTCTGCTGG AGAACTTTCA AGTAGGAGGA ACCTACCAAA TACAGCGGAC 
AGTAGGAGTT TCTCACATGG GCCACAGGAC CCTGGCCAGC TGTGTTCCTC GGCAGAGAAT 
GACCAGGGGA GCGGCACTGC TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_010818556.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010816858.1 Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X7, mRNA.

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CloneID	OBa52961
Clone ID Related Accession (Same CDS sequence)	XM_010818556.2 , XM_015469031.1
Accession Version	XM_010818556.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104561.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818556.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010816858.1
CDS	31..4293
Translation	MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGL

Target ORF information:

RefSeq Version	XM_010818556.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818556.2

ORF Insert Sequence:

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF13A ( XM_015469031.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324517.1 Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X7, mRNA.

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CloneID	OBa52961
Clone ID Related Accession (Same CDS sequence)	XM_010818556.2 , XM_015469031.1
Accession Version	XM_015469031.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF13A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646615.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324517.1
CDS	31..4293
Translation	MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGNAEQRGLIPRLCCALFQRISLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFNIVITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEKLRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLFGIGIQPEHCEIDIGSDGEVSLTPKENARSCVNGTLVCSTTQLWHGDRILWGNNHFFRINLPKRKRRDWLKDFEKETGPPEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNGGFPIISLSAWWSSPWWSYLKILFPLMWFQTKLLHKHWDKDPVQNVVQVLEKQYLEEKRSALEEQRLMYERELEQLRQQLSPERQPQISGPDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREANFLAEEMSKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKGKSTQVWTIEKLENKLIDMRDLYQEWKEKIPEAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGAVPERMAEDDSSENSSESGSLEVVDSTGEIIHRVKKLTCRVKIKEATGLPLNLSNFVFCQYTFWDQCEPTVAAPVVDPEVPSPQSKDAQYTVTFSHCQDYVVNVTEEFLEFISDGALAIEVWGHRCTGNGSSIWEVDSLHAKTRTLHDRWNEVTRRIEMWISILELNELGEYAAVELHQAKDVNTGGVLQLRQGHSRRVQVTVKPVQHSGTLPLMVEAILSISIGCITARSTKLQRGLDSYQRDDEDGDDMDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDDVEREARLVEQWVGLTEERNAVLVPAPGSGIPGAPADWIPPPGMETHIPVLFLDLNADDLSANEQLVGPHASGVNSILPKEHGSQFFYLPIIKHSDEEVSATASWDSSVHDSVHLNRVTPQNERIYLIVKTTVQLSHPAAMELVLRKRIAANIYNKQSFTQSLKRRISLKNIFYSCGVTYEIVSNIPKATEEIEDRETLALMAARSENEGTSDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKARHLRRSLSTPNVHNVSSSRPDLSGFDDDDKGWPENQLDMSDYSPSYQDVSCYGTLPRDSPRRSKEGL

Target ORF information:

RefSeq Version	XM_015469031.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin-like protein KIF13A (LOC534832), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469031.1

ORF Insert Sequence:

ATGGATGAAT CCAACACTAC AAAGTATGCT GGTCAAGAAG TGGTTTTCAA GTGCCTTGGG 
GAAGGAATTC TTGAAAAAGC CTTTCAGGGG TATAATGCTT GCATTTTTGC ATATGGACAG 
ACAGGCTCAG GAAAATCCTT CTCCATGATG GGCAATGCTG AGCAGCGAGG CCTCATCCCA 
AGGCTCTGCT GTGCTTTATT TCAAAGGATC TCTCTGGAGC AGAATGAGTC ACAGACATTT 
AAAGTGGAAG TATCCTATAT GGAAATTTAT AATGAGAAAG TCCGGGACCT GTTAGACCCC 
AAAGGGAGTA GACAGTCTCT TAAAGTTCGA GAGCATAAAG TTTTGGGACC ATATGTAGAT 
GGTTTATCCC AACTGGCTGT CACTAGTTTT GAGGACATTG AGTCATTGAT GTCTGAGGGA 
AACAAATCTC GGACGGTAGC TGCGACCAAC ATGAATGAAG AAAGCAGCCG CTCCCATGCA 
GTGTTCAACA TCGTCATCAC GCAGACGCTG TACGACCTGC AGTCTGGGAA CTCAGGGGAG 
AAGGTGAGCA AGGTGAGCTT GGTGGACCTG GCGGGCAGCG AGAGAGTGTC TAAAACTGGA 
GCTGCGGGAG AGCGCCTGAA AGAAGGCAGC AACATTAACA AATCACTTAC AACCTTGGGG 
TTGGTTATCT CATCGCTGGC TGACCAGGCA GCTGGCAAGG GTAAAAACAA ATTTGTGCCT 
TATCGCGACT CAGTCCTCAC TTGGCTGCTT AAGGACAATC TGGGGGGCAA CAGCCAGACC 
TCCATGATCG CCACCATTAG CCCAGCAGCA GACAACTACG AAGAGACCCT CTCCACGCTG 
AGATACGCAG ATCGGGCCAA AAGGATCGTT AACCATGCCG TCGTGAATGA GGATCCCAAC 
GCGAAGGTCA TCCGGGAACT GCGGGAAGAG GTGGAGAAAC TGAGGGAGCA GCTCTCACAG 
GCTGAGGCTA TGAAGGCCCC TGAACTGAAA GAGAAGCTGG AAGAGTCTGA AAAGCTGATA 
AAAGAGCTAA CAGTGACTTG GGAAGAGAAG CTCAGGAAAA CAGAAGAAAT TGCACAGGAG 
AGGCAGCGGC AACTTGAAAG CATGGGGATC TCCCTGGAGA CTTCTGGTAT CAAAGTAGGA 
GATGACAAGT GCTACTTGGT CAACCTGAAT GCAGACCCTG CTCTCAACGA ACTTCTGGTT 
TATTATTTAA AGGACCACAC CAGGGTAGGT GCCGACACCT CTCAGGACAT CCAGCTCTTT 
GGGATAGGAA TTCAGCCTGA GCACTGTGAG ATCGACATTG GCTCTGATGG AGAGGTCAGC 
CTCACTCCAA AAGAAAATGC ACGGTCCTGT GTCAATGGCA CCCTCGTGTG CAGCACCACC 
CAGCTGTGGC ATGGGGACCG AATCCTGTGG GGAAACAACC ACTTCTTCAG GATTAATTTG 
CCCAAGAGGA AACGGCGAGA TTGGTTGAAA GACTTTGAGA AAGAGACAGG CCCGCCGGAG 
CACGACCTGG ACGCGGCCAG TGAAGCGTCC TCAGAACCAG ACTATAACTA CGAATTTGCG 
CAGATGGAGG TTATCATGAA AACGCTGAAT AGCAATGGTG GCTTCCCGAT CATAAGCCTG 
TCTGCCTGGT GGTCATCCCC TTGGTGGTCC TATTTGAAGA TTCTCTTCCC TCTCATGTGG 
TTTCAGACGA AGCTCCTCCA CAAACACTGG GATAAAGACC CAGTTCAGAA CGTGGTGCAG 
GTCTTGGAGA AACAGTACCT GGAAGAAAAG CGAAGCGCCC TCGAGGAGCA GCGACTCATG 
TACGAGCGGG AGCTGGAGCA GCTCCGCCAG CAGCTGTCCC CGGAGAGGCA GCCCCAGATC 
AGCGGCCCCG ACCGCCTGGC CTACAGCAGC CAGACGGCGC AGCAGAAGGT GACCCAGTGG 
GCCGAGGAGA GGGACGAACT CTTTCGCCAA AGTCTGGCAA AACTTCGGGA GCAGCTGGTG 
AAAGCCAACA CCCTGGTGAG AGAGGCAAAC TTCTTAGCCG AGGAGATGAG CAAGCTCACC 
GACTACCAGG TGACTCTGCA GATCCCTGCT GCGAACCTCA GTGCCAACAG GAAGAGAGGA 
GCAATAGTGA GTGAGCCAGC TATTCAAGTG AGGAGGAAAG GAAAGAGCAC CCAAGTGTGG 
ACCATTGAGA AGCTGGAGAA TAAATTAATT GATATGAGAG ACCTTTATCA AGAATGGAAA 
GAAAAGATTC CTGAGGCAAA GCGACTCTGC GGGAAACGAG GTGACCCTTT CTATGAAGCC 
CAAGAGAATC ACAACCTGAT TGGCGTGGCG AATGTGTTCC TGGAGTGTCT CTTCTGTGAT 
GTGAAGCTTC AGTATGCAGT TCCTATCATC AGCCAGCAGG GGGAGGTTGC AGGGCGTCTC 
CACGTGGAAG TGATGCGAGT GACAGGCGCT GTCCCGGAGC GCATGGCGGA GGACGACTCC 
TCGGAGAACT CCAGTGAAAG CGGAAGTCTT GAAGTCGTAG ACAGCACCGG GGAAATCATT 
CACCGAGTCA AGAAGCTGAC ATGCCGGGTA AAAATTAAAG AAGCAACTGG GCTGCCCTTA 
AACCTCTCAA ATTTCGTCTT CTGTCAATAC ACGTTCTGGG ACCAGTGTGA GCCCACGGTG 
GCCGCCCCTG TCGTGGATCC TGAGGTGCCT TCCCCACAGT CCAAGGACGC CCAGTACACC 
GTGACCTTCT CCCACTGTCA GGATTACGTG GTCAACGTAA CGGAAGAATT CCTGGAGTTC 
ATTTCAGATG GAGCACTGGC CATCGAAGTG TGGGGCCACC GGTGTACCGG GAACGGCAGC 
TCCATCTGGG AAGTCGATTC CCTTCATGCT AAGACGAGAA CGCTGCATGA CAGGTGGAAT 
GAAGTTACTC GCAGAATAGA GATGTGGATC TCCATCCTGG AGCTGAACGA GCTGGGGGAG 
TACGCCGCCG TGGAGCTTCA CCAGGCCAAG GACGTCAACA CAGGCGGCGT CCTTCAGCTC 
AGACAGGGTC ATTCCCGGAG AGTCCAAGTC ACAGTGAAGC CTGTCCAGCA TTCCGGGACA 
CTGCCACTCA TGGTTGAAGC CATCTTGTCC ATCTCCATCG GCTGCATCAC TGCCAGGTCA 
ACCAAGCTCC AGAGAGGGCT GGACAGTTAC CAGAGAGATG ATGAGGATGG TGATGATATG 
GATAGTTATC AGGAAGAAGA CTTAAACTGC GTGAGAGAGA GGTGGTCGGA TGCCCTCATT 
AAACGGCGAG AATACCTAGA CGAACAGATT AAAAAAGTCA GCAACAAAAA AGAGAAGACG 
GAGGATGACG TGGAGCGGGA AGCCCGGCTG GTGGAGCAGT GGGTGGGGCT GACGGAGGAG 
AGGAACGCTG TGCTGGTGCC TGCGCCGGGC AGCGGGATCC CCGGGGCGCC TGCCGACTGG 
ATCCCACCAC CTGGAATGGA AACCCACATA CCGGTTCTCT TCCTTGATTT GAACGCGGAT 
GACCTCAGTG CCAATGAGCA GCTCGTGGGC CCCCACGCAT CAGGCGTGAA CTCCATCCTC 
CCCAAGGAGC ACGGCAGCCA GTTTTTCTAC CTGCCCATCA TAAAGCACAG TGACGAGGAG 
GTTTCAGCCA CAGCCTCCTG GGACTCCTCG GTGCATGATT CCGTTCACTT GAACAGGGTC 
ACCCCGCAGA ATGAGAGGAT CTACCTGATA GTGAAGACCA CAGTCCAGCT CAGCCACCCG 
GCCGCTATGG AGCTGGTCTT ACGAAAACGG ATTGCAGCCA ACATTTACAA CAAACAGAGT 
TTCACCCAGA GTTTGAAGAG GAGAATATCA CTGAAGAATA TATTTTATTC CTGTGGTGTA 
ACCTATGAAA TAGTATCCAA CATACCAAAG GCAACAGAGG AGATTGAGGA CCGGGAGACG 
CTGGCTCTCA TGGCTGCAAG GAGTGAGAAT GAAGGCACGT CGGATGGGGA GACGTACATC 
GAGAAGTACA CGCGGGGCGT GCTGCAGGTG GAGAACATTC TGAGCCTGGA ACGACTCCGG 
CAGGCGGTGA CGGTCAAAGA AGCACTTTCC ACCAAAGCTC GGCACCTACG GCGAAGCCTC 
AGCACGCCCA ACGTTCACAA TGTCTCTTCC AGTCGACCAG ACCTTTCTGG CTTTGACGAT 
GATGATAAGG GTTGGCCAGA GAACCAGTTA GACATGTCTG ACTACAGCCC GAGTTACCAG 
GATGTATCGT GTTATGGAAC TTTGCCTAGG GATTCACCTC GAAGGAGTAA AGAAGGACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bos taurus (cattle)	KIF13A	XP_002697622.3
Mus musculus (house mouse)	Kif13a	NP_034747.2
Xenopus tropicalis (tropical clawed frog)	kif13a	XP_002932725.2
Pan troglodytes (chimpanzee)	KIF13A	XP_527240.2
Rattus norvegicus (Norway rat)	Kif13a	NP_001100932.1
Danio rerio (zebrafish)	kif13a	XP_002665108.2
	KIF13A	XP_001096670.1
Gallus gallus (chicken)	KIF13A	XP_418923.3
Homo sapiens (human)	KIF13A	NP_071396.4
Canis lupus familiaris (dog)	KIF13A	XP_005640119.1
Caenorhabditis elegans (roundworm)	klp-4	NP_508546.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007592	XP_308280.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

KIF13A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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