GTF2I cDNA ORF clone, Bos taurus(cattle)

The following GTF2I gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GTF2I cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa44240 NM_001102177.1
Latest version!
Bos taurus general transcription factor IIi (GTF2I), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa33286 XM_005225172.3
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa33286 XM_015460484.1
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa33286 XM_005225174.3
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa33287 XM_005225175.3
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa33288 XM_005225176.3
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa33289 XM_005225177.3
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa33290 XM_005225178.3
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa44240 XM_024984995.1
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa230339 XM_005225174.4
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa230339 XM_015460484.2
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa230339 XM_005225172.4
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa230340 XM_005225175.4
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa230341 XM_005225176.4
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa230342 XM_005225177.4
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa230343 XM_005225178.4
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa230343 XM_024984996.1
Latest version!
Bos taurus general transcription factor IIi (GTF2I), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa44240
    Clone ID Related Accession (Same CDS sequence) NM_001102177.1 , XM_024984995.1
    Accession Version NM_001102177.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2937bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Bos taurus(cattle)
    Product general transcription factor II-I
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151387.1. On Aug 21, 2007 this sequence version replaced XM_868667.2. ##Evidence-Data-START## Transcript exon combination :: BC151387.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATGATGACTA TCCTCCACCT
    GCCAAGAGAC CAAAGAGCAG TGAACCTCCC CAGCCACCCG TCACAGAACC TGCGAATGCC
    GGCAAACGGA AAGTGAGGGA GTTCAACTTC GAGAAATGGA ATGCTCGAAT TACTGATCTG
    CGTAAACAAG TTGAAGAGTT GTTTGAAAGA AAGTATGCTC AAGCTATAAA AGCCAAAGGT
    CCTGTGACGA TCCCGTACCC TCTTTTCCAG TCCCATGTGG AAGATCTTTA TGTAGAAGGA
    CTTCCAGAAG GAATCCCCTT TAGAAGGCCA TCAACTTATG GAATCCCTCG CCTTGAGAGG
    ATATTACTTG CAAAGGAAAG GATTCGTTTT GTGATTAAGA AGCATGAACT TTTGAATTCA
    ACACGTGAAG ACTTACAGCT TGATAAACCA GCTTCAGGAG TCAAGGAAGA GTGGTATGCC
    AGAATCACTA AGTTACGAAA GATGGTAGAT CAGCTTTTCT GTAAAAAATT TGCTGAAGCC
    TTAGGGAGCA CCGAAGCTAA GGCTGTACCT TACCAGAAAT TTGAAGCACA CCCTAATGAC
    CTCTACGTGG AAGGACTGCC AGAAAACATT CCTTTTAGAA GTCCCTCGTG GTATGGGATC
    CCAAGGCTGG AAAAGATTAT TCAAGTGGGC AATCGAATTA AATTTGTTAT TAAAAGACCA
    GAGCTTCTGA CGCACAGCAC TACTGAAGTT ACTCAGCCCA GAACGAATAC ACCAGTCAAA
    GAAGATTGGA ATGTCAGAAT TACCAAGCTA CGGAAGCAAG TGGAAGAGAT TTTTAATTTG
    AAATTTGCTC AAGCTCTTGG ACTCACAGAA GCAGTAAAAG TACCATACCC GGTGTTTGAA
    TCAAACCCCG AGTTCCTCTA TGTAGAAGGC TTGCCAGAAG GAATTCCCTT TCGAAGCCCT
    ACCTGGTTTG GAATTCCAAG ACTGGAAAGG ATTGTCCGTG GAAGTAATAA AATCAAGTTT
    GTTGTTAAAA AACCTGAACT AGTTATTTCC TACTTGCCTC CTGGAATGGC TAGTAAAATA
    AACACTAAAG CTTTGCAGTC CCCGAAAAGA CCACGAAGCC CCGGGAGTAA TTCGAAAGTT
    CCTGAAATTG AGGTCACTGT GGAAGGCCCT AATAATAGCA ATCCTCAAAC CTCAGCCGTT
    CGAACCCCTA CCCAGACGAA TGGTTCTAAT GTTCCCTTCA AGCCTCGAGG GAGAGAGTTT
    TCCTTTGAGG CCTGGAATGC CAAAATCACA GACCTAAAAC AGAAAGTTGA AAATCTTTTC
    AATGAAAAAT GTGGTGAAGC TCTTGGCCTT AAACAAGCTG TGAAAGTGCC GTTCGCGTTA
    TTTGAGTCTT TCCCAGAAGA CTTTTATGTG GAAGGCTTAC CCGAGGGTGT GCCATTCCGA
    CGACCGTCTA CTTTTGGCAT TCCAAGGCTG GAGAAGATAC TCAGAAACAA AGCCAAAATT
    AAGTTCATCA TTAAAAAGCC AGAAATGTTT GAGACTGCAA TTAAGGAGAG CACCTCCTCA
    TCTAAGAGCC CTCCAAGAAA AATCAATTCA TCACCCAGTG TTAATACTAC TGCATCAGGT
    GTTGAAGACC TTAACATCAT TCAGGTGACA ATTCCAGACG ATGATAATGA AAGACTATCA
    AAAGTTGAAA AAGCTCGACA GCTAAGAGAG CAAGTTAATG ACCTCTTCAG TCGAAAATTT
    GGTGAAGCCA TTGGGATGGG TTTCCCCGTG AAGGTTCCTT ACAGGAAAAT CACAATTAAC
    CCTGGCTGTG TGGTGGTTGA TGGCATGCCC CCCGGAGTGT CATTCAAAGC CCCCAGCTAT
    CTGGAAATCA GCTCCATGAG AAGGATCTTA GATTCTGCTG AGTTCATTAA ATTCACAGTC
    ATTAGACCAT TTCCAGGACT TGTGATTAAT AACCAGTTGG TTGATCAGAA TGAGTCAGAA
    GGCCCAGTGA TCCAAGAATC AGCTGAGCCA ACCCAGTTGG AGGTTCCTGC AACCGAAGAA
    ATAAAGGAGA CTGATGGAAA CTCCCAAATC AAGCAAGAAC CAGACCCTAC GTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001095647.1
    CDS356..3292
    Translation

    Target ORF information:

    RefSeq Version NM_001102177.1
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102177.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATGATGACTA TCCTCCACCT
    GCCAAGAGAC CAAAGAGCAG TGAACCTCCC CAGCCACCCG TCACAGAACC TGCGAATGCC
    GGCAAACGGA AAGTGAGGGA GTTCAACTTC GAGAAATGGA ATGCTCGAAT TACTGATCTG
    CGTAAACAAG TTGAAGAGTT GTTTGAAAGA AAGTATGCTC AAGCTATAAA AGCCAAAGGT
    CCTGTGACGA TCCCGTACCC TCTTTTCCAG TCCCATGTGG AAGATCTTTA TGTAGAAGGA
    CTTCCAGAAG GAATCCCCTT TAGAAGGCCA TCAACTTATG GAATCCCTCG CCTTGAGAGG
    ATATTACTTG CAAAGGAAAG GATTCGTTTT GTGATTAAGA AGCATGAACT TTTGAATTCA
    ACACGTGAAG ACTTACAGCT TGATAAACCA GCTTCAGGAG TCAAGGAAGA GTGGTATGCC
    AGAATCACTA AGTTACGAAA GATGGTAGAT CAGCTTTTCT GTAAAAAATT TGCTGAAGCC
    TTAGGGAGCA CCGAAGCTAA GGCTGTACCT TACCAGAAAT TTGAAGCACA CCCTAATGAC
    CTCTACGTGG AAGGACTGCC AGAAAACATT CCTTTTAGAA GTCCCTCGTG GTATGGGATC
    CCAAGGCTGG AAAAGATTAT TCAAGTGGGC AATCGAATTA AATTTGTTAT TAAAAGACCA
    GAGCTTCTGA CGCACAGCAC TACTGAAGTT ACTCAGCCCA GAACGAATAC ACCAGTCAAA
    GAAGATTGGA ATGTCAGAAT TACCAAGCTA CGGAAGCAAG TGGAAGAGAT TTTTAATTTG
    AAATTTGCTC AAGCTCTTGG ACTCACAGAA GCAGTAAAAG TACCATACCC GGTGTTTGAA
    TCAAACCCCG AGTTCCTCTA TGTAGAAGGC TTGCCAGAAG GAATTCCCTT TCGAAGCCCT
    ACCTGGTTTG GAATTCCAAG ACTGGAAAGG ATTGTCCGTG GAAGTAATAA AATCAAGTTT
    GTTGTTAAAA AACCTGAACT AGTTATTTCC TACTTGCCTC CTGGAATGGC TAGTAAAATA
    AACACTAAAG CTTTGCAGTC CCCGAAAAGA CCACGAAGCC CCGGGAGTAA TTCGAAAGTT
    CCTGAAATTG AGGTCACTGT GGAAGGCCCT AATAATAGCA ATCCTCAAAC CTCAGCCGTT
    CGAACCCCTA CCCAGACGAA TGGTTCTAAT GTTCCCTTCA AGCCTCGAGG GAGAGAGTTT
    TCCTTTGAGG CCTGGAATGC CAAAATCACA GACCTAAAAC AGAAAGTTGA AAATCTTTTC
    AATGAAAAAT GTGGTGAAGC TCTTGGCCTT AAACAAGCTG TGAAAGTGCC GTTCGCGTTA
    TTTGAGTCTT TCCCAGAAGA CTTTTATGTG GAAGGCTTAC CCGAGGGTGT GCCATTCCGA
    CGACCGTCTA CTTTTGGCAT TCCAAGGCTG GAGAAGATAC TCAGAAACAA AGCCAAAATT
    AAGTTCATCA TTAAAAAGCC AGAAATGTTT GAGACTGCAA TTAAGGAGAG CACCTCCTCA
    TCTAAGAGCC CTCCAAGAAA AATCAATTCA TCACCCAGTG TTAATACTAC TGCATCAGGT
    GTTGAAGACC TTAACATCAT TCAGGTGACA ATTCCAGACG ATGATAATGA AAGACTATCA
    AAAGTTGAAA AAGCTCGACA GCTAAGAGAG CAAGTTAATG ACCTCTTCAG TCGAAAATTT
    GGTGAAGCCA TTGGGATGGG TTTCCCCGTG AAGGTTCCTT ACAGGAAAAT CACAATTAAC
    CCTGGCTGTG TGGTGGTTGA TGGCATGCCC CCCGGAGTGT CATTCAAAGC CCCCAGCTAT
    CTGGAAATCA GCTCCATGAG AAGGATCTTA GATTCTGCTG AGTTCATTAA ATTCACAGTC
    ATTAGACCAT TTCCAGGACT TGTGATTAAT AACCAGTTGG TTGATCAGAA TGAGTCAGAA
    GGCCCAGTGA TCCAAGAATC AGCTGAGCCA ACCCAGTTGG AGGTTCCTGC AACCGAAGAA
    ATAAAGGAGA CTGATGGAAA CTCCCAAATC AAGCAAGAAC CAGACCCTAC GTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33286
    Clone ID Related Accession (Same CDS sequence) XM_015460484.1 , XM_005225172.3 , XM_005225174.3
    Accession Version XM_015460484.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTCTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315970.1
    CDS456..3455
    Translation

    Target ORF information:

    RefSeq Version XM_015460484.1
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460484.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTCTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33286
    Clone ID Related Accession (Same CDS sequence) XM_015460484.1 , XM_005225172.3 , XM_005225174.3
    Accession Version XM_005225172.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225172.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTCTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225229.1
    CDS384..3383
    Translation

    Target ORF information:

    RefSeq Version XM_005225172.3
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225172.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTCTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33286
    Clone ID Related Accession (Same CDS sequence) XM_015460484.1 , XM_005225172.3 , XM_005225174.3
    Accession Version XM_005225174.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225174.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTCTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225231.1
    CDS384..3383
    Translation

    Target ORF information:

    RefSeq Version XM_005225174.3
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225174.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTCTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33287
    Clone ID Related Accession (Same CDS sequence) XM_005225175.3
    Accession Version XM_005225175.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225175.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGCCCTTC TGAAACTGAT
    GATGTTGATG ACAAACTCCC CCTTTCGAAG TCTTTGCAAG GAAGCCATCA TTCTTCAGAG
    GGCAATGAAG GAACAGAAAT GGAAGTACCA GCAGAAGATT CTACTCAACA TGTCCCTTCA
    GAAACAAGTG AGGACCCCGA AGTTGAGGTG ACTATTGAAG ATGATGACTA TCCTCCACCT
    GCCAAGAGAC CAAAGAGCAG TGAACCTCCC CAGCCACCCG TCACAGAACC TGCGAATGCC
    GGCAAACGGA AAGTGAGGGA GTTCAACTTC GAGAAATGGA ATGCTCGAAT TACTGATCTG
    CGTAAACAAG TTGAAGAGTT GTTTGAAAGA AAGTATGCTC AAGCTATAAA AGCCAAAGGT
    CCTGTGACGA TCCCGTACCC TCTTTTCCAG TCCCATGTGG AAGATCTTTA TGTAGAAGGA
    CTTCCAGAAG GAATCCCCTT TAGAAGGCCA TCAACTTATG GAATCCCTCG CCTTGAGAGG
    ATATTACTTG CAAAGGAAAG GATTCGTTTT GTGATTAAGA AGCATGAACT TCTGAATTCA
    ACACGTGAAG ACTTACAGCT TGATAAACCA GCTTCAGGAG TCAAGGAAGA GTGGTATGCC
    AGAATCACTA AGTTACGAAA GATGGTAGAT CAGCTTTTCT GTAAAAAATT TGCTGAAGCC
    TTAGGGAGCA CCGAAGCTAA GGCTGTACCT TACCAGAAAT TTGAAGCACA CCCTAATGAC
    CTCTACGTGG AAGGACTGCC AGAAAACATT CCTTTTAGAA GTCCCTCGTG GTATGGGATC
    CCAAGGCTGG AAAAGATTAT TCAAGTGGGC AATCGAATTA AATTTGTTAT TAAAAGACCA
    GAGCTTCTGA CGCACAGCAC TACTGAAGTT ACTCAGCCCA GAACGAATAC ACCAGTCAAA
    GAAGATTGGA ATGTCAGAAT TACCAAGCTA CGGAAGCAAG TGGAAGAGAT TTTTAATTTG
    AAATTTGCTC AAGCTCTTGG ACTCACAGAA GCAGTAAAAG TACCATACCC GGTGTTTGAA
    TCAAACCCCG AGTTCCTCTA TGTAGAAGGC TTGCCAGAAG GAATTCCCTT TCGAAGCCCT
    ACCTGGTTTG GAATTCCAAG ACTGGAAAGG ATTGTCCGTG GAAGTAATAA AATCAAGTTT
    GTTGTTAAAA AACCTGAACT AGTTATTTCC TACTTGCCTC CTGGAATGGC TAGTAAAATA
    AACACTAAAG CTTTGCAGTC CCCGAAAAGA CCACGAAGCC CCGGGAGTAA TTCGAAAGTT
    CCTGAAATTG AGGTCACTGT GGAAGGCCCT AATAATAGCA ATCCTCAAAC CTCAGCCGTT
    CGAACCCCTA CCCAGACGAA TGGTTCTAAT GTTCCCTTCA AGCCTCGAGG GAGAGAGTTT
    TCCTTTGAGG CCTGGAATGC CAAAATCACA GACCTAAAAC AGAAAGTTGA AAATCTTTTC
    AATGAAAAAT GTGGTGAAGC TCTTGGCCTT AAACAAGCTG TGAAAGTGCC GTTCGCGTTA
    TTTGAGTCTT TCCCAGAAGA CTTTTATGTG GAAGGCTTAC CCGAGGGTGT GCCATTCCGA
    CGACCGTCTA CTTTTGGCAT TCCAAGGCTG GAGAAGATAC TCAGAAACAA AGCCAAAATT
    AAGTTCATCA TTAAAAAGCC AGAAATGTTT GAGACTGCAA TTAAGGAGAG CACCTCCTCA
    TCTAAGAGCC CTCCAAGAAA AATCAATTCA TCACCCAGTG TTAATACTAC TGCATCAGGT
    GTTGAAGACC TTAACATCAT TCAGGTGACA ATTCCAGACG ATGATAATGA AAGACTATCA
    AAAGTTGAAA AAGCTCGACA GCTAAGAGAG CAAGTTAATG ACCTCTTCAG TCGAAAATTT
    GGTGAAGCCA TTGGGATGGG TTTCCCCGTG AAGGTTCCTT ACAGGAAAAT CACAATTAAC
    CCTGGCTGTG TGGTGGTTGA TGGCATGCCC CCCGGAGTGT CATTCAAAGC CCCCAGCTAT
    CTGGAAATCA GCTCCATGAG AAGGATCTTA GATTCTGCTG AGTTCATTAA ATTCACAGTC
    ATTAGACCAT TTCCAGGACT TGTGATTAAT AACCAGTTGG TTGATCAGAA TGAGTCAGAA
    GGCCCAGTGA TCCAAGAATC AGCTGAGCCA ACCCAGTTGG AGGTTCCTGC AACCGAAGAA
    ATAAAGGAGA CTGATGGAAA CTCCCAAATC AAGCAAGAAC CAGACCCTAC GTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225232.1
    CDS384..3380
    Translation

    Target ORF information:

    RefSeq Version XM_005225175.3
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225175.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGCCCTTC TGAAACTGAT
    GATGTTGATG ACAAACTCCC CCTTTCGAAG TCTTTGCAAG GAAGCCATCA TTCTTCAGAG
    GGCAATGAAG GAACAGAAAT GGAAGTACCA GCAGAAGATT CTACTCAACA TGTCCCTTCA
    GAAACAAGTG AGGACCCCGA AGTTGAGGTG ACTATTGAAG ATGATGACTA TCCTCCACCT
    GCCAAGAGAC CAAAGAGCAG TGAACCTCCC CAGCCACCCG TCACAGAACC TGCGAATGCC
    GGCAAACGGA AAGTGAGGGA GTTCAACTTC GAGAAATGGA ATGCTCGAAT TACTGATCTG
    CGTAAACAAG TTGAAGAGTT GTTTGAAAGA AAGTATGCTC AAGCTATAAA AGCCAAAGGT
    CCTGTGACGA TCCCGTACCC TCTTTTCCAG TCCCATGTGG AAGATCTTTA TGTAGAAGGA
    CTTCCAGAAG GAATCCCCTT TAGAAGGCCA TCAACTTATG GAATCCCTCG CCTTGAGAGG
    ATATTACTTG CAAAGGAAAG GATTCGTTTT GTGATTAAGA AGCATGAACT TCTGAATTCA
    ACACGTGAAG ACTTACAGCT TGATAAACCA GCTTCAGGAG TCAAGGAAGA GTGGTATGCC
    AGAATCACTA AGTTACGAAA GATGGTAGAT CAGCTTTTCT GTAAAAAATT TGCTGAAGCC
    TTAGGGAGCA CCGAAGCTAA GGCTGTACCT TACCAGAAAT TTGAAGCACA CCCTAATGAC
    CTCTACGTGG AAGGACTGCC AGAAAACATT CCTTTTAGAA GTCCCTCGTG GTATGGGATC
    CCAAGGCTGG AAAAGATTAT TCAAGTGGGC AATCGAATTA AATTTGTTAT TAAAAGACCA
    GAGCTTCTGA CGCACAGCAC TACTGAAGTT ACTCAGCCCA GAACGAATAC ACCAGTCAAA
    GAAGATTGGA ATGTCAGAAT TACCAAGCTA CGGAAGCAAG TGGAAGAGAT TTTTAATTTG
    AAATTTGCTC AAGCTCTTGG ACTCACAGAA GCAGTAAAAG TACCATACCC GGTGTTTGAA
    TCAAACCCCG AGTTCCTCTA TGTAGAAGGC TTGCCAGAAG GAATTCCCTT TCGAAGCCCT
    ACCTGGTTTG GAATTCCAAG ACTGGAAAGG ATTGTCCGTG GAAGTAATAA AATCAAGTTT
    GTTGTTAAAA AACCTGAACT AGTTATTTCC TACTTGCCTC CTGGAATGGC TAGTAAAATA
    AACACTAAAG CTTTGCAGTC CCCGAAAAGA CCACGAAGCC CCGGGAGTAA TTCGAAAGTT
    CCTGAAATTG AGGTCACTGT GGAAGGCCCT AATAATAGCA ATCCTCAAAC CTCAGCCGTT
    CGAACCCCTA CCCAGACGAA TGGTTCTAAT GTTCCCTTCA AGCCTCGAGG GAGAGAGTTT
    TCCTTTGAGG CCTGGAATGC CAAAATCACA GACCTAAAAC AGAAAGTTGA AAATCTTTTC
    AATGAAAAAT GTGGTGAAGC TCTTGGCCTT AAACAAGCTG TGAAAGTGCC GTTCGCGTTA
    TTTGAGTCTT TCCCAGAAGA CTTTTATGTG GAAGGCTTAC CCGAGGGTGT GCCATTCCGA
    CGACCGTCTA CTTTTGGCAT TCCAAGGCTG GAGAAGATAC TCAGAAACAA AGCCAAAATT
    AAGTTCATCA TTAAAAAGCC AGAAATGTTT GAGACTGCAA TTAAGGAGAG CACCTCCTCA
    TCTAAGAGCC CTCCAAGAAA AATCAATTCA TCACCCAGTG TTAATACTAC TGCATCAGGT
    GTTGAAGACC TTAACATCAT TCAGGTGACA ATTCCAGACG ATGATAATGA AAGACTATCA
    AAAGTTGAAA AAGCTCGACA GCTAAGAGAG CAAGTTAATG ACCTCTTCAG TCGAAAATTT
    GGTGAAGCCA TTGGGATGGG TTTCCCCGTG AAGGTTCCTT ACAGGAAAAT CACAATTAAC
    CCTGGCTGTG TGGTGGTTGA TGGCATGCCC CCCGGAGTGT CATTCAAAGC CCCCAGCTAT
    CTGGAAATCA GCTCCATGAG AAGGATCTTA GATTCTGCTG AGTTCATTAA ATTCACAGTC
    ATTAGACCAT TTCCAGGACT TGTGATTAAT AACCAGTTGG TTGATCAGAA TGAGTCAGAA
    GGCCCAGTGA TCCAAGAATC AGCTGAGCCA ACCCAGTTGG AGGTTCCTGC AACCGAAGAA
    ATAAAGGAGA CTGATGGAAA CTCCCAAATC AAGCAAGAAC CAGACCCTAC GTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33288
    Clone ID Related Accession (Same CDS sequence) XM_005225176.3
    Accession Version XM_005225176.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225176.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTCTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225233.1
    CDS384..3323
    Translation

    Target ORF information:

    RefSeq Version XM_005225176.3
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225176.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTCTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33289
    Clone ID Related Accession (Same CDS sequence) XM_005225177.3
    Accession Version XM_005225177.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225177.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGCCCTTC TGAAACTGAT
    GATGTTGATG ACAAACTCCC CCTTTCGAAG TCTTTGCAAG GAAGCCATCA TTCTTCAGAG
    GGCAATGAAG GAACAGAAAT GGAAGTACCA GCAGAAGATG ATGACTATCC TCCACCTGCC
    AAGAGACCAA AGAGCAGTGA ACCTCCCCAG CCACCCGTCA CAGAACCTGC GAATGCCGGC
    AAACGGAAAG TGAGGGAGTT CAACTTCGAG AAATGGAATG CTCGAATTAC TGATCTGCGT
    AAACAAGTTG AAGAGTTGTT TGAAAGAAAG TATGCTCAAG CTATAAAAGC CAAAGGTCCT
    GTGACGATCC CGTACCCTCT TTTCCAGTCC CATGTGGAAG ATCTTTATGT AGAAGGACTT
    CCAGAAGGAA TCCCCTTTAG AAGGCCATCA ACTTATGGAA TCCCTCGCCT TGAGAGGATA
    TTACTTGCAA AGGAAAGGAT TCGTTTTGTG ATTAAGAAGC ATGAACTTCT GAATTCAACA
    CGTGAAGACT TACAGCTTGA TAAACCAGCT TCAGGAGTCA AGGAAGAGTG GTATGCCAGA
    ATCACTAAGT TACGAAAGAT GGTAGATCAG CTTTTCTGTA AAAAATTTGC TGAAGCCTTA
    GGGAGCACCG AAGCTAAGGC TGTACCTTAC CAGAAATTTG AAGCACACCC TAATGACCTC
    TACGTGGAAG GACTGCCAGA AAACATTCCT TTTAGAAGTC CCTCGTGGTA TGGGATCCCA
    AGGCTGGAAA AGATTATTCA AGTGGGCAAT CGAATTAAAT TTGTTATTAA AAGACCAGAG
    CTTCTGACGC ACAGCACTAC TGAAGTTACT CAGCCCAGAA CGAATACACC AGTCAAAGAA
    GATTGGAATG TCAGAATTAC CAAGCTACGG AAGCAAGTGG AAGAGATTTT TAATTTGAAA
    TTTGCTCAAG CTCTTGGACT CACAGAAGCA GTAAAAGTAC CATACCCGGT GTTTGAATCA
    AACCCCGAGT TCCTCTATGT AGAAGGCTTG CCAGAAGGAA TTCCCTTTCG AAGCCCTACC
    TGGTTTGGAA TTCCAAGACT GGAAAGGATT GTCCGTGGAA GTAATAAAAT CAAGTTTGTT
    GTTAAAAAAC CTGAACTAGT TATTTCCTAC TTGCCTCCTG GAATGGCTAG TAAAATAAAC
    ACTAAAGCTT TGCAGTCCCC GAAAAGACCA CGAAGCCCCG GGAGTAATTC GAAAGTTCCT
    GAAATTGAGG TCACTGTGGA AGGCCCTAAT AATAGCAATC CTCAAACCTC AGCCGTTCGA
    ACCCCTACCC AGACGAATGG TTCTAATGTT CCCTTCAAGC CTCGAGGGAG AGAGTTTTCC
    TTTGAGGCCT GGAATGCCAA AATCACAGAC CTAAAACAGA AAGTTGAAAA TCTTTTCAAT
    GAAAAATGTG GTGAAGCTCT TGGCCTTAAA CAAGCTGTGA AAGTGCCGTT CGCGTTATTT
    GAGTCTTTCC CAGAAGACTT TTATGTGGAA GGCTTACCCG AGGGTGTGCC ATTCCGACGA
    CCGTCTACTT TTGGCATTCC AAGGCTGGAG AAGATACTCA GAAACAAAGC CAAAATTAAG
    TTCATCATTA AAAAGCCAGA AATGTTTGAG ACTGCAATTA AGGAGAGCAC CTCCTCATCT
    AAGAGCCCTC CAAGAAAAAT CAATTCATCA CCCAGTGTTA ATACTACTGC ATCAGGTGTT
    GAAGACCTTA ACATCATTCA GGTGACAATT CCAGACGATG ATAATGAAAG ACTATCAAAA
    GTTGAAAAAG CTCGACAGCT AAGAGAGCAA GTTAATGACC TCTTCAGTCG AAAATTTGGT
    GAAGCCATTG GGATGGGTTT CCCCGTGAAG GTTCCTTACA GGAAAATCAC AATTAACCCT
    GGCTGTGTGG TGGTTGATGG CATGCCCCCC GGAGTGTCAT TCAAAGCCCC CAGCTATCTG
    GAAATCAGCT CCATGAGAAG GATCTTAGAT TCTGCTGAGT TCATTAAATT CACAGTCATT
    AGACCATTTC CAGGACTTGT GATTAATAAC CAGTTGGTTG ATCAGAATGA GTCAGAAGGC
    CCAGTGATCC AAGAATCAGC TGAGCCAACC CAGTTGGAGG TTCCTGCAAC CGAAGAAATA
    AAGGAGACTG ATGGAAACTC CCAAATCAAG CAAGAACCAG ACCCTACGTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225234.1
    CDS384..3317
    Translation

    Target ORF information:

    RefSeq Version XM_005225177.3
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225177.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGCCCTTC TGAAACTGAT
    GATGTTGATG ACAAACTCCC CCTTTCGAAG TCTTTGCAAG GAAGCCATCA TTCTTCAGAG
    GGCAATGAAG GAACAGAAAT GGAAGTACCA GCAGAAGATG ATGACTATCC TCCACCTGCC
    AAGAGACCAA AGAGCAGTGA ACCTCCCCAG CCACCCGTCA CAGAACCTGC GAATGCCGGC
    AAACGGAAAG TGAGGGAGTT CAACTTCGAG AAATGGAATG CTCGAATTAC TGATCTGCGT
    AAACAAGTTG AAGAGTTGTT TGAAAGAAAG TATGCTCAAG CTATAAAAGC CAAAGGTCCT
    GTGACGATCC CGTACCCTCT TTTCCAGTCC CATGTGGAAG ATCTTTATGT AGAAGGACTT
    CCAGAAGGAA TCCCCTTTAG AAGGCCATCA ACTTATGGAA TCCCTCGCCT TGAGAGGATA
    TTACTTGCAA AGGAAAGGAT TCGTTTTGTG ATTAAGAAGC ATGAACTTCT GAATTCAACA
    CGTGAAGACT TACAGCTTGA TAAACCAGCT TCAGGAGTCA AGGAAGAGTG GTATGCCAGA
    ATCACTAAGT TACGAAAGAT GGTAGATCAG CTTTTCTGTA AAAAATTTGC TGAAGCCTTA
    GGGAGCACCG AAGCTAAGGC TGTACCTTAC CAGAAATTTG AAGCACACCC TAATGACCTC
    TACGTGGAAG GACTGCCAGA AAACATTCCT TTTAGAAGTC CCTCGTGGTA TGGGATCCCA
    AGGCTGGAAA AGATTATTCA AGTGGGCAAT CGAATTAAAT TTGTTATTAA AAGACCAGAG
    CTTCTGACGC ACAGCACTAC TGAAGTTACT CAGCCCAGAA CGAATACACC AGTCAAAGAA
    GATTGGAATG TCAGAATTAC CAAGCTACGG AAGCAAGTGG AAGAGATTTT TAATTTGAAA
    TTTGCTCAAG CTCTTGGACT CACAGAAGCA GTAAAAGTAC CATACCCGGT GTTTGAATCA
    AACCCCGAGT TCCTCTATGT AGAAGGCTTG CCAGAAGGAA TTCCCTTTCG AAGCCCTACC
    TGGTTTGGAA TTCCAAGACT GGAAAGGATT GTCCGTGGAA GTAATAAAAT CAAGTTTGTT
    GTTAAAAAAC CTGAACTAGT TATTTCCTAC TTGCCTCCTG GAATGGCTAG TAAAATAAAC
    ACTAAAGCTT TGCAGTCCCC GAAAAGACCA CGAAGCCCCG GGAGTAATTC GAAAGTTCCT
    GAAATTGAGG TCACTGTGGA AGGCCCTAAT AATAGCAATC CTCAAACCTC AGCCGTTCGA
    ACCCCTACCC AGACGAATGG TTCTAATGTT CCCTTCAAGC CTCGAGGGAG AGAGTTTTCC
    TTTGAGGCCT GGAATGCCAA AATCACAGAC CTAAAACAGA AAGTTGAAAA TCTTTTCAAT
    GAAAAATGTG GTGAAGCTCT TGGCCTTAAA CAAGCTGTGA AAGTGCCGTT CGCGTTATTT
    GAGTCTTTCC CAGAAGACTT TTATGTGGAA GGCTTACCCG AGGGTGTGCC ATTCCGACGA
    CCGTCTACTT TTGGCATTCC AAGGCTGGAG AAGATACTCA GAAACAAAGC CAAAATTAAG
    TTCATCATTA AAAAGCCAGA AATGTTTGAG ACTGCAATTA AGGAGAGCAC CTCCTCATCT
    AAGAGCCCTC CAAGAAAAAT CAATTCATCA CCCAGTGTTA ATACTACTGC ATCAGGTGTT
    GAAGACCTTA ACATCATTCA GGTGACAATT CCAGACGATG ATAATGAAAG ACTATCAAAA
    GTTGAAAAAG CTCGACAGCT AAGAGAGCAA GTTAATGACC TCTTCAGTCG AAAATTTGGT
    GAAGCCATTG GGATGGGTTT CCCCGTGAAG GTTCCTTACA GGAAAATCAC AATTAACCCT
    GGCTGTGTGG TGGTTGATGG CATGCCCCCC GGAGTGTCAT TCAAAGCCCC CAGCTATCTG
    GAAATCAGCT CCATGAGAAG GATCTTAGAT TCTGCTGAGT TCATTAAATT CACAGTCATT
    AGACCATTTC CAGGACTTGT GATTAATAAC CAGTTGGTTG ATCAGAATGA GTCAGAAGGC
    CCAGTGATCC AAGAATCAGC TGAGCCAACC CAGTTGGAGG TTCCTGCAAC CGAAGAAATA
    AAGGAGACTG ATGGAAACTC CCAAATCAAG CAAGAACCAG ACCCTACGTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33290
    Clone ID Related Accession (Same CDS sequence) XM_005225178.3
    Accession Version XM_005225178.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2877bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225178.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATGATGACTA TCCTCCACCT
    GCCAAGAGAC CAAAGAGCAG TGAACCTCCC CAGCCACCCG TCACAGAACC TGCGAATGCC
    GGCAAACGGA AAGTGAGGGA GTTCAACTTC GAGAAATGGA ATGCTCGAAT TACTGATCTG
    CGTAAACAAG TTGAAGAGTT GTTTGAAAGA AAGTATGCTC AAGCTATAAA AGCCAAAGGT
    CCTGTGACGA TCCCGTACCC TCTTTTCCAG TCCCATGTGG AAGATCTTTA TGTAGAAGGA
    CTTCCAGAAG GAATCCCCTT TAGAAGGCCA TCAACTTATG GAATCCCTCG CCTTGAGAGG
    ATATTACTTG CAAAGGAAAG GATTCGTTTT GTGATTAAGA AGCATGAACT TCTGAATTCA
    ACACGTGAAG ACTTACAGCT TGATAAACCA GCTTCAGGAG TCAAGGAAGA GTGGTATGCC
    AGAATCACTA AGTTACGAAA GATGGTAGAT CAGCTTTTCT GTAAAAAATT TGCTGAAGCC
    TTAGGGAGCA CCGAAGCTAA GGCTGTACCT TACCAGAAAT TTGAAGCACA CCCTAATGAC
    CTCTACGTGG AAGGACTGCC AGAAAACATT CCTTTTAGAA GTCCCTCGTG GTATGGGATC
    CCAAGGCTGG AAAAGATTAT TCAAGTGGGC AATCGAATTA AATTTGTTAT TAAAAGACCA
    GAGCTTCTGA CGCACAGCAC TACTGAAGTT ACTCAGCCCA GAACGAATAC ACCAGTCAAA
    GAAGATTGGA ATGTCAGAAT TACCAAGCTA CGGAAGCAAG TGGAAGAGAT TTTTAATTTG
    AAATTTGCTC AAGCTCTTGG ACTCACAGAA GCAGTAAAAG TACCATACCC GGTGTTTGAA
    TCAAACCCCG AGTTCCTCTA TGTAGAAGGC TTGCCAGAAG GAATTCCCTT TCGAAGCCCT
    ACCTGGTTTG GAATTCCAAG ACTGGAAAGG ATTGTCCGTG GAAGTAATAA AATCAAGTTT
    GTTGTTAAAA AACCTGAACT AGTTATTTCC TACTTGCCTC CTGGAATGGC TAGTAAAATA
    AACACTAAAG CTTTGCAGTC CCCGAAAAGA CCACGAAGCC CCGGGAGTAA TTCGAAAGTT
    CCTGAAATTG AGGTCACTGT GGAAGGCCCT AATAATAGCA ATCCTCAAAC CTCAGCCGTT
    CGAACCCCTA CCCAGACGAA TGGTTCTAAT GTTCCCTTCA AGCCTCGAGG GAGAGAGTTT
    TCCTTTGAGG CCTGGAATGC CAAAATCACA GACCTAAAAC AGAAAGTTGA AAATCTTTTC
    AATGAAAAAT GTGGTGAAGC TCTTGGCCTT AAACAAGCTG TGAAAGTGCC GTTCGCGTTA
    TTTGAGTCTT TCCCAGAAGA CTTTTATGTG GAAGGCTTAC CCGAGGGTGT GCCATTCCGA
    CGACCGTCTA CTTTTGGCAT TCCAAGGCTG GAGAAGATAC TCAGAAACAA AGCCAAAATT
    AAGTTCATCA TTAAAAAGCC AGAAATGTTT GAGACTGCAA TTAAGGAGAG CACCTCCTCA
    TCTAAGAGCC CTCCAAGAAA AATCAATTCA TCACCCAGTG TTAATACTAC TGCATCAGGT
    GTTGAAGACC TTAACATCAT TCAGGTGACA ATTCCAGACG ATGATAATGA AAGACTATCA
    AAAGTTGAAA AAGCTCGACA GCTAAGAGAG CAAGTTAATG ACCTCTTCAG TCGAAAATTT
    GGTGAAGCCA TTGGGATGGG TTTCCCCGTG AAGGTTCCTT ACAGGAAAAT CACAATTAAC
    CCTGGCTGTG TGGTGGTTGA TGGCATGCCC CCCGGAGTGT CATTCAAAGC CCCCAGCTAT
    CTGGAAATCA GCTCCATGAG AAGGATCTTA GATTCTGCTG AGTTCATTAA ATTCACAGTC
    ATTAGACCAT TTCCAGGACT TGTGATTAAT AACCAGTTGG TTGATCAGAA TGAGTCAGAA
    GGCCCAGTGA TCCAAGAATC AGCTGAGCCA ACCCAGTTGG AGGTTCCTGC AACCGAAGAA
    ATAAAGGAGA CTGATGGAAA CTCCCAAATC AAGCAAGAAC CAGACCCTAC GTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225235.1
    CDS384..3260
    Translation

    Target ORF information:

    RefSeq Version XM_005225178.3
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225178.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATGATGACTA TCCTCCACCT
    GCCAAGAGAC CAAAGAGCAG TGAACCTCCC CAGCCACCCG TCACAGAACC TGCGAATGCC
    GGCAAACGGA AAGTGAGGGA GTTCAACTTC GAGAAATGGA ATGCTCGAAT TACTGATCTG
    CGTAAACAAG TTGAAGAGTT GTTTGAAAGA AAGTATGCTC AAGCTATAAA AGCCAAAGGT
    CCTGTGACGA TCCCGTACCC TCTTTTCCAG TCCCATGTGG AAGATCTTTA TGTAGAAGGA
    CTTCCAGAAG GAATCCCCTT TAGAAGGCCA TCAACTTATG GAATCCCTCG CCTTGAGAGG
    ATATTACTTG CAAAGGAAAG GATTCGTTTT GTGATTAAGA AGCATGAACT TCTGAATTCA
    ACACGTGAAG ACTTACAGCT TGATAAACCA GCTTCAGGAG TCAAGGAAGA GTGGTATGCC
    AGAATCACTA AGTTACGAAA GATGGTAGAT CAGCTTTTCT GTAAAAAATT TGCTGAAGCC
    TTAGGGAGCA CCGAAGCTAA GGCTGTACCT TACCAGAAAT TTGAAGCACA CCCTAATGAC
    CTCTACGTGG AAGGACTGCC AGAAAACATT CCTTTTAGAA GTCCCTCGTG GTATGGGATC
    CCAAGGCTGG AAAAGATTAT TCAAGTGGGC AATCGAATTA AATTTGTTAT TAAAAGACCA
    GAGCTTCTGA CGCACAGCAC TACTGAAGTT ACTCAGCCCA GAACGAATAC ACCAGTCAAA
    GAAGATTGGA ATGTCAGAAT TACCAAGCTA CGGAAGCAAG TGGAAGAGAT TTTTAATTTG
    AAATTTGCTC AAGCTCTTGG ACTCACAGAA GCAGTAAAAG TACCATACCC GGTGTTTGAA
    TCAAACCCCG AGTTCCTCTA TGTAGAAGGC TTGCCAGAAG GAATTCCCTT TCGAAGCCCT
    ACCTGGTTTG GAATTCCAAG ACTGGAAAGG ATTGTCCGTG GAAGTAATAA AATCAAGTTT
    GTTGTTAAAA AACCTGAACT AGTTATTTCC TACTTGCCTC CTGGAATGGC TAGTAAAATA
    AACACTAAAG CTTTGCAGTC CCCGAAAAGA CCACGAAGCC CCGGGAGTAA TTCGAAAGTT
    CCTGAAATTG AGGTCACTGT GGAAGGCCCT AATAATAGCA ATCCTCAAAC CTCAGCCGTT
    CGAACCCCTA CCCAGACGAA TGGTTCTAAT GTTCCCTTCA AGCCTCGAGG GAGAGAGTTT
    TCCTTTGAGG CCTGGAATGC CAAAATCACA GACCTAAAAC AGAAAGTTGA AAATCTTTTC
    AATGAAAAAT GTGGTGAAGC TCTTGGCCTT AAACAAGCTG TGAAAGTGCC GTTCGCGTTA
    TTTGAGTCTT TCCCAGAAGA CTTTTATGTG GAAGGCTTAC CCGAGGGTGT GCCATTCCGA
    CGACCGTCTA CTTTTGGCAT TCCAAGGCTG GAGAAGATAC TCAGAAACAA AGCCAAAATT
    AAGTTCATCA TTAAAAAGCC AGAAATGTTT GAGACTGCAA TTAAGGAGAG CACCTCCTCA
    TCTAAGAGCC CTCCAAGAAA AATCAATTCA TCACCCAGTG TTAATACTAC TGCATCAGGT
    GTTGAAGACC TTAACATCAT TCAGGTGACA ATTCCAGACG ATGATAATGA AAGACTATCA
    AAAGTTGAAA AAGCTCGACA GCTAAGAGAG CAAGTTAATG ACCTCTTCAG TCGAAAATTT
    GGTGAAGCCA TTGGGATGGG TTTCCCCGTG AAGGTTCCTT ACAGGAAAAT CACAATTAAC
    CCTGGCTGTG TGGTGGTTGA TGGCATGCCC CCCGGAGTGT CATTCAAAGC CCCCAGCTAT
    CTGGAAATCA GCTCCATGAG AAGGATCTTA GATTCTGCTG AGTTCATTAA ATTCACAGTC
    ATTAGACCAT TTCCAGGACT TGTGATTAAT AACCAGTTGG TTGATCAGAA TGAGTCAGAA
    GGCCCAGTGA TCCAAGAATC AGCTGAGCCA ACCCAGTTGG AGGTTCCTGC AACCGAAGAA
    ATAAAGGAGA CTGATGGAAA CTCCCAAATC AAGCAAGAAC CAGACCCTAC GTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa44240
    Clone ID Related Accession (Same CDS sequence) NM_001102177.1 , XM_024984995.1
    Accession Version XM_024984995.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2937bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATGATGACTA TCCTCCACCT
    GCCAAGAGAC CAAAGAGCAG TGAACCTCCC CAGCCACCCG TCACAGAACC TGCGAATGCC
    GGCAAACGGA AAGTGAGGGA GTTCAACTTC GAGAAATGGA ATGCTCGAAT TACTGATCTG
    CGTAAACAAG TTGAAGAGTT GTTTGAAAGA AAGTATGCTC AAGCTATAAA AGCCAAAGGT
    CCTGTGACGA TCCCGTACCC TCTTTTCCAG TCCCATGTGG AAGATCTTTA TGTAGAAGGA
    CTTCCAGAAG GAATCCCCTT TAGAAGGCCA TCAACTTATG GAATCCCTCG CCTTGAGAGG
    ATATTACTTG CAAAGGAAAG GATTCGTTTT GTGATTAAGA AGCATGAACT TTTGAATTCA
    ACACGTGAAG ACTTACAGCT TGATAAACCA GCTTCAGGAG TCAAGGAAGA GTGGTATGCC
    AGAATCACTA AGTTACGAAA GATGGTAGAT CAGCTTTTCT GTAAAAAATT TGCTGAAGCC
    TTAGGGAGCA CCGAAGCTAA GGCTGTACCT TACCAGAAAT TTGAAGCACA CCCTAATGAC
    CTCTACGTGG AAGGACTGCC AGAAAACATT CCTTTTAGAA GTCCCTCGTG GTATGGGATC
    CCAAGGCTGG AAAAGATTAT TCAAGTGGGC AATCGAATTA AATTTGTTAT TAAAAGACCA
    GAGCTTCTGA CGCACAGCAC TACTGAAGTT ACTCAGCCCA GAACGAATAC ACCAGTCAAA
    GAAGATTGGA ATGTCAGAAT TACCAAGCTA CGGAAGCAAG TGGAAGAGAT TTTTAATTTG
    AAATTTGCTC AAGCTCTTGG ACTCACAGAA GCAGTAAAAG TACCATACCC GGTGTTTGAA
    TCAAACCCCG AGTTCCTCTA TGTAGAAGGC TTGCCAGAAG GAATTCCCTT TCGAAGCCCT
    ACCTGGTTTG GAATTCCAAG ACTGGAAAGG ATTGTCCGTG GAAGTAATAA AATCAAGTTT
    GTTGTTAAAA AACCTGAACT AGTTATTTCC TACTTGCCTC CTGGAATGGC TAGTAAAATA
    AACACTAAAG CTTTGCAGTC CCCGAAAAGA CCACGAAGCC CCGGGAGTAA TTCGAAAGTT
    CCTGAAATTG AGGTCACTGT GGAAGGCCCT AATAATAGCA ATCCTCAAAC CTCAGCCGTT
    CGAACCCCTA CCCAGACGAA TGGTTCTAAT GTTCCCTTCA AGCCTCGAGG GAGAGAGTTT
    TCCTTTGAGG CCTGGAATGC CAAAATCACA GACCTAAAAC AGAAAGTTGA AAATCTTTTC
    AATGAAAAAT GTGGTGAAGC TCTTGGCCTT AAACAAGCTG TGAAAGTGCC GTTCGCGTTA
    TTTGAGTCTT TCCCAGAAGA CTTTTATGTG GAAGGCTTAC CCGAGGGTGT GCCATTCCGA
    CGACCGTCTA CTTTTGGCAT TCCAAGGCTG GAGAAGATAC TCAGAAACAA AGCCAAAATT
    AAGTTCATCA TTAAAAAGCC AGAAATGTTT GAGACTGCAA TTAAGGAGAG CACCTCCTCA
    TCTAAGAGCC CTCCAAGAAA AATCAATTCA TCACCCAGTG TTAATACTAC TGCATCAGGT
    GTTGAAGACC TTAACATCAT TCAGGTGACA ATTCCAGACG ATGATAATGA AAGACTATCA
    AAAGTTGAAA AAGCTCGACA GCTAAGAGAG CAAGTTAATG ACCTCTTCAG TCGAAAATTT
    GGTGAAGCCA TTGGGATGGG TTTCCCCGTG AAGGTTCCTT ACAGGAAAAT CACAATTAAC
    CCTGGCTGTG TGGTGGTTGA TGGCATGCCC CCCGGAGTGT CATTCAAAGC CCCCAGCTAT
    CTGGAAATCA GCTCCATGAG AAGGATCTTA GATTCTGCTG AGTTCATTAA ATTCACAGTC
    ATTAGACCAT TTCCAGGACT TGTGATTAAT AACCAGTTGG TTGATCAGAA TGAGTCAGAA
    GGCCCAGTGA TCCAAGAATC AGCTGAGCCA ACCCAGTTGG AGGTTCCTGC AACCGAAGAA
    ATAAAGGAGA CTGATGGAAA CTCCCAAATC AAGCAAGAAC CAGACCCTAC GTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840763.1
    CDS462..3398
    Translation

    Target ORF information:

    RefSeq Version XM_024984995.1
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984995.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATGATGACTA TCCTCCACCT
    GCCAAGAGAC CAAAGAGCAG TGAACCTCCC CAGCCACCCG TCACAGAACC TGCGAATGCC
    GGCAAACGGA AAGTGAGGGA GTTCAACTTC GAGAAATGGA ATGCTCGAAT TACTGATCTG
    CGTAAACAAG TTGAAGAGTT GTTTGAAAGA AAGTATGCTC AAGCTATAAA AGCCAAAGGT
    CCTGTGACGA TCCCGTACCC TCTTTTCCAG TCCCATGTGG AAGATCTTTA TGTAGAAGGA
    CTTCCAGAAG GAATCCCCTT TAGAAGGCCA TCAACTTATG GAATCCCTCG CCTTGAGAGG
    ATATTACTTG CAAAGGAAAG GATTCGTTTT GTGATTAAGA AGCATGAACT TTTGAATTCA
    ACACGTGAAG ACTTACAGCT TGATAAACCA GCTTCAGGAG TCAAGGAAGA GTGGTATGCC
    AGAATCACTA AGTTACGAAA GATGGTAGAT CAGCTTTTCT GTAAAAAATT TGCTGAAGCC
    TTAGGGAGCA CCGAAGCTAA GGCTGTACCT TACCAGAAAT TTGAAGCACA CCCTAATGAC
    CTCTACGTGG AAGGACTGCC AGAAAACATT CCTTTTAGAA GTCCCTCGTG GTATGGGATC
    CCAAGGCTGG AAAAGATTAT TCAAGTGGGC AATCGAATTA AATTTGTTAT TAAAAGACCA
    GAGCTTCTGA CGCACAGCAC TACTGAAGTT ACTCAGCCCA GAACGAATAC ACCAGTCAAA
    GAAGATTGGA ATGTCAGAAT TACCAAGCTA CGGAAGCAAG TGGAAGAGAT TTTTAATTTG
    AAATTTGCTC AAGCTCTTGG ACTCACAGAA GCAGTAAAAG TACCATACCC GGTGTTTGAA
    TCAAACCCCG AGTTCCTCTA TGTAGAAGGC TTGCCAGAAG GAATTCCCTT TCGAAGCCCT
    ACCTGGTTTG GAATTCCAAG ACTGGAAAGG ATTGTCCGTG GAAGTAATAA AATCAAGTTT
    GTTGTTAAAA AACCTGAACT AGTTATTTCC TACTTGCCTC CTGGAATGGC TAGTAAAATA
    AACACTAAAG CTTTGCAGTC CCCGAAAAGA CCACGAAGCC CCGGGAGTAA TTCGAAAGTT
    CCTGAAATTG AGGTCACTGT GGAAGGCCCT AATAATAGCA ATCCTCAAAC CTCAGCCGTT
    CGAACCCCTA CCCAGACGAA TGGTTCTAAT GTTCCCTTCA AGCCTCGAGG GAGAGAGTTT
    TCCTTTGAGG CCTGGAATGC CAAAATCACA GACCTAAAAC AGAAAGTTGA AAATCTTTTC
    AATGAAAAAT GTGGTGAAGC TCTTGGCCTT AAACAAGCTG TGAAAGTGCC GTTCGCGTTA
    TTTGAGTCTT TCCCAGAAGA CTTTTATGTG GAAGGCTTAC CCGAGGGTGT GCCATTCCGA
    CGACCGTCTA CTTTTGGCAT TCCAAGGCTG GAGAAGATAC TCAGAAACAA AGCCAAAATT
    AAGTTCATCA TTAAAAAGCC AGAAATGTTT GAGACTGCAA TTAAGGAGAG CACCTCCTCA
    TCTAAGAGCC CTCCAAGAAA AATCAATTCA TCACCCAGTG TTAATACTAC TGCATCAGGT
    GTTGAAGACC TTAACATCAT TCAGGTGACA ATTCCAGACG ATGATAATGA AAGACTATCA
    AAAGTTGAAA AAGCTCGACA GCTAAGAGAG CAAGTTAATG ACCTCTTCAG TCGAAAATTT
    GGTGAAGCCA TTGGGATGGG TTTCCCCGTG AAGGTTCCTT ACAGGAAAAT CACAATTAAC
    CCTGGCTGTG TGGTGGTTGA TGGCATGCCC CCCGGAGTGT CATTCAAAGC CCCCAGCTAT
    CTGGAAATCA GCTCCATGAG AAGGATCTTA GATTCTGCTG AGTTCATTAA ATTCACAGTC
    ATTAGACCAT TTCCAGGACT TGTGATTAAT AACCAGTTGG TTGATCAGAA TGAGTCAGAA
    GGCCCAGTGA TCCAAGAATC AGCTGAGCCA ACCCAGTTGG AGGTTCCTGC AACCGAAGAA
    ATAAAGGAGA CTGATGGAAA CTCCCAAATC AAGCAAGAAC CAGACCCTAC GTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230339
    Clone ID Related Accession (Same CDS sequence) XM_005225172.4 , XM_015460484.2 , XM_005225174.4
    Accession Version XM_005225172.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225172.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTTTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225229.1
    CDS397..3396
    Translation

    Target ORF information:

    RefSeq Version XM_005225172.4
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225172.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTTTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230339
    Clone ID Related Accession (Same CDS sequence) XM_005225172.4 , XM_015460484.2 , XM_005225174.4
    Accession Version XM_015460484.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460484.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTTTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315970.1
    CDS462..3461
    Translation

    Target ORF information:

    RefSeq Version XM_015460484.2
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460484.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTTTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230339
    Clone ID Related Accession (Same CDS sequence) XM_005225172.4 , XM_015460484.2 , XM_005225174.4
    Accession Version XM_005225174.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225174.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTTTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225231.1
    CDS390..3389
    Translation

    Target ORF information:

    RefSeq Version XM_005225174.4
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225174.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGCAGGCCC TTCTGAAACT
    GATGATGTTG ATGACAAACT CCCCCTTTCG AAGTCTTTGC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTTTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230340
    Clone ID Related Accession (Same CDS sequence) XM_005225175.4
    Accession Version XM_005225175.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225175.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGCCCTTC TGAAACTGAT
    GATGTTGATG ACAAACTCCC CCTTTCGAAG TCTTTGCAAG GAAGCCATCA TTCTTCAGAG
    GGCAATGAAG GAACAGAAAT GGAAGTACCA GCAGAAGATT CTACTCAACA TGTCCCTTCA
    GAAACAAGTG AGGACCCCGA AGTTGAGGTG ACTATTGAAG ATGATGACTA TCCTCCACCT
    GCCAAGAGAC CAAAGAGCAG TGAACCTCCC CAGCCACCCG TCACAGAACC TGCGAATGCC
    GGCAAACGGA AAGTGAGGGA GTTCAACTTC GAGAAATGGA ATGCTCGAAT TACTGATCTG
    CGTAAACAAG TTGAAGAGTT GTTTGAAAGA AAGTATGCTC AAGCTATAAA AGCCAAAGGT
    CCTGTGACGA TCCCGTACCC TCTTTTCCAG TCCCATGTGG AAGATCTTTA TGTAGAAGGA
    CTTCCAGAAG GAATCCCCTT TAGAAGGCCA TCAACTTATG GAATCCCTCG CCTTGAGAGG
    ATATTACTTG CAAAGGAAAG GATTCGTTTT GTGATTAAGA AGCATGAACT TTTGAATTCA
    ACACGTGAAG ACTTACAGCT TGATAAACCA GCTTCAGGAG TCAAGGAAGA GTGGTATGCC
    AGAATCACTA AGTTACGAAA GATGGTAGAT CAGCTTTTCT GTAAAAAATT TGCTGAAGCC
    TTAGGGAGCA CCGAAGCTAA GGCTGTACCT TACCAGAAAT TTGAAGCACA CCCTAATGAC
    CTCTACGTGG AAGGACTGCC AGAAAACATT CCTTTTAGAA GTCCCTCGTG GTATGGGATC
    CCAAGGCTGG AAAAGATTAT TCAAGTGGGC AATCGAATTA AATTTGTTAT TAAAAGACCA
    GAGCTTCTGA CGCACAGCAC TACTGAAGTT ACTCAGCCCA GAACGAATAC ACCAGTCAAA
    GAAGATTGGA ATGTCAGAAT TACCAAGCTA CGGAAGCAAG TGGAAGAGAT TTTTAATTTG
    AAATTTGCTC AAGCTCTTGG ACTCACAGAA GCAGTAAAAG TACCATACCC GGTGTTTGAA
    TCAAACCCCG AGTTCCTCTA TGTAGAAGGC TTGCCAGAAG GAATTCCCTT TCGAAGCCCT
    ACCTGGTTTG GAATTCCAAG ACTGGAAAGG ATTGTCCGTG GAAGTAATAA AATCAAGTTT
    GTTGTTAAAA AACCTGAACT AGTTATTTCC TACTTGCCTC CTGGAATGGC TAGTAAAATA
    AACACTAAAG CTTTGCAGTC CCCGAAAAGA CCACGAAGCC CCGGGAGTAA TTCGAAAGTT
    CCTGAAATTG AGGTCACTGT GGAAGGCCCT AATAATAGCA ATCCTCAAAC CTCAGCCGTT
    CGAACCCCTA CCCAGACGAA TGGTTCTAAT GTTCCCTTCA AGCCTCGAGG GAGAGAGTTT
    TCCTTTGAGG CCTGGAATGC CAAAATCACA GACCTAAAAC AGAAAGTTGA AAATCTTTTC
    AATGAAAAAT GTGGTGAAGC TCTTGGCCTT AAACAAGCTG TGAAAGTGCC GTTCGCGTTA
    TTTGAGTCTT TCCCAGAAGA CTTTTATGTG GAAGGCTTAC CCGAGGGTGT GCCATTCCGA
    CGACCGTCTA CTTTTGGCAT TCCAAGGCTG GAGAAGATAC TCAGAAACAA AGCCAAAATT
    AAGTTCATCA TTAAAAAGCC AGAAATGTTT GAGACTGCAA TTAAGGAGAG CACCTCCTCA
    TCTAAGAGCC CTCCAAGAAA AATCAATTCA TCACCCAGTG TTAATACTAC TGCATCAGGT
    GTTGAAGACC TTAACATCAT TCAGGTGACA ATTCCAGACG ATGATAATGA AAGACTATCA
    AAAGTTGAAA AAGCTCGACA GCTAAGAGAG CAAGTTAATG ACCTCTTCAG TCGAAAATTT
    GGTGAAGCCA TTGGGATGGG TTTCCCCGTG AAGGTTCCTT ACAGGAAAAT CACAATTAAC
    CCTGGCTGTG TGGTGGTTGA TGGCATGCCC CCCGGAGTGT CATTCAAAGC CCCCAGCTAT
    CTGGAAATCA GCTCCATGAG AAGGATCTTA GATTCTGCTG AGTTCATTAA ATTCACAGTC
    ATTAGACCAT TTCCAGGACT TGTGATTAAT AACCAGTTGG TTGATCAGAA TGAGTCAGAA
    GGCCCAGTGA TCCAAGAATC AGCTGAGCCA ACCCAGTTGG AGGTTCCTGC AACCGAAGAA
    ATAAAGGAGA CTGATGGAAA CTCCCAAATC AAGCAAGAAC CAGACCCTAC GTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225232.1
    CDS390..3386
    Translation

    Target ORF information:

    RefSeq Version XM_005225175.4
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225175.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGCCCTTC TGAAACTGAT
    GATGTTGATG ACAAACTCCC CCTTTCGAAG TCTTTGCAAG GAAGCCATCA TTCTTCAGAG
    GGCAATGAAG GAACAGAAAT GGAAGTACCA GCAGAAGATT CTACTCAACA TGTCCCTTCA
    GAAACAAGTG AGGACCCCGA AGTTGAGGTG ACTATTGAAG ATGATGACTA TCCTCCACCT
    GCCAAGAGAC CAAAGAGCAG TGAACCTCCC CAGCCACCCG TCACAGAACC TGCGAATGCC
    GGCAAACGGA AAGTGAGGGA GTTCAACTTC GAGAAATGGA ATGCTCGAAT TACTGATCTG
    CGTAAACAAG TTGAAGAGTT GTTTGAAAGA AAGTATGCTC AAGCTATAAA AGCCAAAGGT
    CCTGTGACGA TCCCGTACCC TCTTTTCCAG TCCCATGTGG AAGATCTTTA TGTAGAAGGA
    CTTCCAGAAG GAATCCCCTT TAGAAGGCCA TCAACTTATG GAATCCCTCG CCTTGAGAGG
    ATATTACTTG CAAAGGAAAG GATTCGTTTT GTGATTAAGA AGCATGAACT TTTGAATTCA
    ACACGTGAAG ACTTACAGCT TGATAAACCA GCTTCAGGAG TCAAGGAAGA GTGGTATGCC
    AGAATCACTA AGTTACGAAA GATGGTAGAT CAGCTTTTCT GTAAAAAATT TGCTGAAGCC
    TTAGGGAGCA CCGAAGCTAA GGCTGTACCT TACCAGAAAT TTGAAGCACA CCCTAATGAC
    CTCTACGTGG AAGGACTGCC AGAAAACATT CCTTTTAGAA GTCCCTCGTG GTATGGGATC
    CCAAGGCTGG AAAAGATTAT TCAAGTGGGC AATCGAATTA AATTTGTTAT TAAAAGACCA
    GAGCTTCTGA CGCACAGCAC TACTGAAGTT ACTCAGCCCA GAACGAATAC ACCAGTCAAA
    GAAGATTGGA ATGTCAGAAT TACCAAGCTA CGGAAGCAAG TGGAAGAGAT TTTTAATTTG
    AAATTTGCTC AAGCTCTTGG ACTCACAGAA GCAGTAAAAG TACCATACCC GGTGTTTGAA
    TCAAACCCCG AGTTCCTCTA TGTAGAAGGC TTGCCAGAAG GAATTCCCTT TCGAAGCCCT
    ACCTGGTTTG GAATTCCAAG ACTGGAAAGG ATTGTCCGTG GAAGTAATAA AATCAAGTTT
    GTTGTTAAAA AACCTGAACT AGTTATTTCC TACTTGCCTC CTGGAATGGC TAGTAAAATA
    AACACTAAAG CTTTGCAGTC CCCGAAAAGA CCACGAAGCC CCGGGAGTAA TTCGAAAGTT
    CCTGAAATTG AGGTCACTGT GGAAGGCCCT AATAATAGCA ATCCTCAAAC CTCAGCCGTT
    CGAACCCCTA CCCAGACGAA TGGTTCTAAT GTTCCCTTCA AGCCTCGAGG GAGAGAGTTT
    TCCTTTGAGG CCTGGAATGC CAAAATCACA GACCTAAAAC AGAAAGTTGA AAATCTTTTC
    AATGAAAAAT GTGGTGAAGC TCTTGGCCTT AAACAAGCTG TGAAAGTGCC GTTCGCGTTA
    TTTGAGTCTT TCCCAGAAGA CTTTTATGTG GAAGGCTTAC CCGAGGGTGT GCCATTCCGA
    CGACCGTCTA CTTTTGGCAT TCCAAGGCTG GAGAAGATAC TCAGAAACAA AGCCAAAATT
    AAGTTCATCA TTAAAAAGCC AGAAATGTTT GAGACTGCAA TTAAGGAGAG CACCTCCTCA
    TCTAAGAGCC CTCCAAGAAA AATCAATTCA TCACCCAGTG TTAATACTAC TGCATCAGGT
    GTTGAAGACC TTAACATCAT TCAGGTGACA ATTCCAGACG ATGATAATGA AAGACTATCA
    AAAGTTGAAA AAGCTCGACA GCTAAGAGAG CAAGTTAATG ACCTCTTCAG TCGAAAATTT
    GGTGAAGCCA TTGGGATGGG TTTCCCCGTG AAGGTTCCTT ACAGGAAAAT CACAATTAAC
    CCTGGCTGTG TGGTGGTTGA TGGCATGCCC CCCGGAGTGT CATTCAAAGC CCCCAGCTAT
    CTGGAAATCA GCTCCATGAG AAGGATCTTA GATTCTGCTG AGTTCATTAA ATTCACAGTC
    ATTAGACCAT TTCCAGGACT TGTGATTAAT AACCAGTTGG TTGATCAGAA TGAGTCAGAA
    GGCCCAGTGA TCCAAGAATC AGCTGAGCCA ACCCAGTTGG AGGTTCCTGC AACCGAAGAA
    ATAAAGGAGA CTGATGGAAA CTCCCAAATC AAGCAAGAAC CAGACCCTAC GTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230341
    Clone ID Related Accession (Same CDS sequence) XM_005225176.4
    Accession Version XM_005225176.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225176.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTTTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225233.1
    CDS394..3333
    Translation

    Target ORF information:

    RefSeq Version XM_005225176.4
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225176.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATTCTACTCA ACATGTCCCT
    TCAGAAACAA GTGAGGACCC CGAAGTTGAG GTGACTATTG AAGATGATGA CTATCCTCCA
    CCTGCCAAGA GACCAAAGAG CAGTGAACCT CCCCAGCCAC CCGTCACAGA ACCTGCGAAT
    GCCGGCAAAC GGAAAGTGAG GGAGTTCAAC TTCGAGAAAT GGAATGCTCG AATTACTGAT
    CTGCGTAAAC AAGTTGAAGA GTTGTTTGAA AGAAAGTATG CTCAAGCTAT AAAAGCCAAA
    GGTCCTGTGA CGATCCCGTA CCCTCTTTTC CAGTCCCATG TGGAAGATCT TTATGTAGAA
    GGACTTCCAG AAGGAATCCC CTTTAGAAGG CCATCAACTT ATGGAATCCC TCGCCTTGAG
    AGGATATTAC TTGCAAAGGA AAGGATTCGT TTTGTGATTA AGAAGCATGA ACTTTTGAAT
    TCAACACGTG AAGACTTACA GCTTGATAAA CCAGCTTCAG GAGTCAAGGA AGAGTGGTAT
    GCCAGAATCA CTAAGTTACG AAAGATGGTA GATCAGCTTT TCTGTAAAAA ATTTGCTGAA
    GCCTTAGGGA GCACCGAAGC TAAGGCTGTA CCTTACCAGA AATTTGAAGC ACACCCTAAT
    GACCTCTACG TGGAAGGACT GCCAGAAAAC ATTCCTTTTA GAAGTCCCTC GTGGTATGGG
    ATCCCAAGGC TGGAAAAGAT TATTCAAGTG GGCAATCGAA TTAAATTTGT TATTAAAAGA
    CCAGAGCTTC TGACGCACAG CACTACTGAA GTTACTCAGC CCAGAACGAA TACACCAGTC
    AAAGAAGATT GGAATGTCAG AATTACCAAG CTACGGAAGC AAGTGGAAGA GATTTTTAAT
    TTGAAATTTG CTCAAGCTCT TGGACTCACA GAAGCAGTAA AAGTACCATA CCCGGTGTTT
    GAATCAAACC CCGAGTTCCT CTATGTAGAA GGCTTGCCAG AAGGAATTCC CTTTCGAAGC
    CCTACCTGGT TTGGAATTCC AAGACTGGAA AGGATTGTCC GTGGAAGTAA TAAAATCAAG
    TTTGTTGTTA AAAAACCTGA ACTAGTTATT TCCTACTTGC CTCCTGGAAT GGCTAGTAAA
    ATAAACACTA AAGCTTTGCA GTCCCCGAAA AGACCACGAA GCCCCGGGAG TAATTCGAAA
    GTTCCTGAAA TTGAGGTCAC TGTGGAAGGC CCTAATAATA GCAATCCTCA AACCTCAGCC
    GTTCGAACCC CTACCCAGAC GAATGGTTCT AATGTTCCCT TCAAGCCTCG AGGGAGAGAG
    TTTTCCTTTG AGGCCTGGAA TGCCAAAATC ACAGACCTAA AACAGAAAGT TGAAAATCTT
    TTCAATGAAA AATGTGGTGA AGCTCTTGGC CTTAAACAAG CTGTGAAAGT GCCGTTCGCG
    TTATTTGAGT CTTTCCCAGA AGACTTTTAT GTGGAAGGCT TACCCGAGGG TGTGCCATTC
    CGACGACCGT CTACTTTTGG CATTCCAAGG CTGGAGAAGA TACTCAGAAA CAAAGCCAAA
    ATTAAGTTCA TCATTAAAAA GCCAGAAATG TTTGAGACTG CAATTAAGGA GAGCACCTCC
    TCATCTAAGA GCCCTCCAAG AAAAATCAAT TCATCACCCA GTGTTAATAC TACTGCATCA
    GGTGTTGAAG ACCTTAACAT CATTCAGGTG ACAATTCCAG ACGATGATAA TGAAAGACTA
    TCAAAAGTTG AAAAAGCTCG ACAGCTAAGA GAGCAAGTTA ATGACCTCTT CAGTCGAAAA
    TTTGGTGAAG CCATTGGGAT GGGTTTCCCC GTGAAGGTTC CTTACAGGAA AATCACAATT
    AACCCTGGCT GTGTGGTGGT TGATGGCATG CCCCCCGGAG TGTCATTCAA AGCCCCCAGC
    TATCTGGAAA TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTCAT TAAATTCACA
    GTCATTAGAC CATTTCCAGG ACTTGTGATT AATAACCAGT TGGTTGATCA GAATGAGTCA
    GAAGGCCCAG TGATCCAAGA ATCAGCTGAG CCAACCCAGT TGGAGGTTCC TGCAACCGAA
    GAAATAAAGG AGACTGATGG AAACTCCCAA ATCAAGCAAG AACCAGACCC TACGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230342
    Clone ID Related Accession (Same CDS sequence) XM_005225177.4
    Accession Version XM_005225177.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225177.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGCCCTTC TGAAACTGAT
    GATGTTGATG ACAAACTCCC CCTTTCGAAG TCTTTGCAAG GAAGCCATCA TTCTTCAGAG
    GGCAATGAAG GAACAGAAAT GGAAGTACCA GCAGAAGATG ATGACTATCC TCCACCTGCC
    AAGAGACCAA AGAGCAGTGA ACCTCCCCAG CCACCCGTCA CAGAACCTGC GAATGCCGGC
    AAACGGAAAG TGAGGGAGTT CAACTTCGAG AAATGGAATG CTCGAATTAC TGATCTGCGT
    AAACAAGTTG AAGAGTTGTT TGAAAGAAAG TATGCTCAAG CTATAAAAGC CAAAGGTCCT
    GTGACGATCC CGTACCCTCT TTTCCAGTCC CATGTGGAAG ATCTTTATGT AGAAGGACTT
    CCAGAAGGAA TCCCCTTTAG AAGGCCATCA ACTTATGGAA TCCCTCGCCT TGAGAGGATA
    TTACTTGCAA AGGAAAGGAT TCGTTTTGTG ATTAAGAAGC ATGAACTTTT GAATTCAACA
    CGTGAAGACT TACAGCTTGA TAAACCAGCT TCAGGAGTCA AGGAAGAGTG GTATGCCAGA
    ATCACTAAGT TACGAAAGAT GGTAGATCAG CTTTTCTGTA AAAAATTTGC TGAAGCCTTA
    GGGAGCACCG AAGCTAAGGC TGTACCTTAC CAGAAATTTG AAGCACACCC TAATGACCTC
    TACGTGGAAG GACTGCCAGA AAACATTCCT TTTAGAAGTC CCTCGTGGTA TGGGATCCCA
    AGGCTGGAAA AGATTATTCA AGTGGGCAAT CGAATTAAAT TTGTTATTAA AAGACCAGAG
    CTTCTGACGC ACAGCACTAC TGAAGTTACT CAGCCCAGAA CGAATACACC AGTCAAAGAA
    GATTGGAATG TCAGAATTAC CAAGCTACGG AAGCAAGTGG AAGAGATTTT TAATTTGAAA
    TTTGCTCAAG CTCTTGGACT CACAGAAGCA GTAAAAGTAC CATACCCGGT GTTTGAATCA
    AACCCCGAGT TCCTCTATGT AGAAGGCTTG CCAGAAGGAA TTCCCTTTCG AAGCCCTACC
    TGGTTTGGAA TTCCAAGACT GGAAAGGATT GTCCGTGGAA GTAATAAAAT CAAGTTTGTT
    GTTAAAAAAC CTGAACTAGT TATTTCCTAC TTGCCTCCTG GAATGGCTAG TAAAATAAAC
    ACTAAAGCTT TGCAGTCCCC GAAAAGACCA CGAAGCCCCG GGAGTAATTC GAAAGTTCCT
    GAAATTGAGG TCACTGTGGA AGGCCCTAAT AATAGCAATC CTCAAACCTC AGCCGTTCGA
    ACCCCTACCC AGACGAATGG TTCTAATGTT CCCTTCAAGC CTCGAGGGAG AGAGTTTTCC
    TTTGAGGCCT GGAATGCCAA AATCACAGAC CTAAAACAGA AAGTTGAAAA TCTTTTCAAT
    GAAAAATGTG GTGAAGCTCT TGGCCTTAAA CAAGCTGTGA AAGTGCCGTT CGCGTTATTT
    GAGTCTTTCC CAGAAGACTT TTATGTGGAA GGCTTACCCG AGGGTGTGCC ATTCCGACGA
    CCGTCTACTT TTGGCATTCC AAGGCTGGAG AAGATACTCA GAAACAAAGC CAAAATTAAG
    TTCATCATTA AAAAGCCAGA AATGTTTGAG ACTGCAATTA AGGAGAGCAC CTCCTCATCT
    AAGAGCCCTC CAAGAAAAAT CAATTCATCA CCCAGTGTTA ATACTACTGC ATCAGGTGTT
    GAAGACCTTA ACATCATTCA GGTGACAATT CCAGACGATG ATAATGAAAG ACTATCAAAA
    GTTGAAAAAG CTCGACAGCT AAGAGAGCAA GTTAATGACC TCTTCAGTCG AAAATTTGGT
    GAAGCCATTG GGATGGGTTT CCCCGTGAAG GTTCCTTACA GGAAAATCAC AATTAACCCT
    GGCTGTGTGG TGGTTGATGG CATGCCCCCC GGAGTGTCAT TCAAAGCCCC CAGCTATCTG
    GAAATCAGCT CCATGAGAAG GATCTTAGAT TCTGCTGAGT TCATTAAATT CACAGTCATT
    AGACCATTTC CAGGACTTGT GATTAATAAC CAGTTGGTTG ATCAGAATGA GTCAGAAGGC
    CCAGTGATCC AAGAATCAGC TGAGCCAACC CAGTTGGAGG TTCCTGCAAC CGAAGAAATA
    AAGGAGACTG ATGGAAACTC CCAAATCAAG CAAGAACCAG ACCCTACGTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225234.1
    CDS390..3323
    Translation

    Target ORF information:

    RefSeq Version XM_005225177.4
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225177.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGCCCTTC TGAAACTGAT
    GATGTTGATG ACAAACTCCC CCTTTCGAAG TCTTTGCAAG GAAGCCATCA TTCTTCAGAG
    GGCAATGAAG GAACAGAAAT GGAAGTACCA GCAGAAGATG ATGACTATCC TCCACCTGCC
    AAGAGACCAA AGAGCAGTGA ACCTCCCCAG CCACCCGTCA CAGAACCTGC GAATGCCGGC
    AAACGGAAAG TGAGGGAGTT CAACTTCGAG AAATGGAATG CTCGAATTAC TGATCTGCGT
    AAACAAGTTG AAGAGTTGTT TGAAAGAAAG TATGCTCAAG CTATAAAAGC CAAAGGTCCT
    GTGACGATCC CGTACCCTCT TTTCCAGTCC CATGTGGAAG ATCTTTATGT AGAAGGACTT
    CCAGAAGGAA TCCCCTTTAG AAGGCCATCA ACTTATGGAA TCCCTCGCCT TGAGAGGATA
    TTACTTGCAA AGGAAAGGAT TCGTTTTGTG ATTAAGAAGC ATGAACTTTT GAATTCAACA
    CGTGAAGACT TACAGCTTGA TAAACCAGCT TCAGGAGTCA AGGAAGAGTG GTATGCCAGA
    ATCACTAAGT TACGAAAGAT GGTAGATCAG CTTTTCTGTA AAAAATTTGC TGAAGCCTTA
    GGGAGCACCG AAGCTAAGGC TGTACCTTAC CAGAAATTTG AAGCACACCC TAATGACCTC
    TACGTGGAAG GACTGCCAGA AAACATTCCT TTTAGAAGTC CCTCGTGGTA TGGGATCCCA
    AGGCTGGAAA AGATTATTCA AGTGGGCAAT CGAATTAAAT TTGTTATTAA AAGACCAGAG
    CTTCTGACGC ACAGCACTAC TGAAGTTACT CAGCCCAGAA CGAATACACC AGTCAAAGAA
    GATTGGAATG TCAGAATTAC CAAGCTACGG AAGCAAGTGG AAGAGATTTT TAATTTGAAA
    TTTGCTCAAG CTCTTGGACT CACAGAAGCA GTAAAAGTAC CATACCCGGT GTTTGAATCA
    AACCCCGAGT TCCTCTATGT AGAAGGCTTG CCAGAAGGAA TTCCCTTTCG AAGCCCTACC
    TGGTTTGGAA TTCCAAGACT GGAAAGGATT GTCCGTGGAA GTAATAAAAT CAAGTTTGTT
    GTTAAAAAAC CTGAACTAGT TATTTCCTAC TTGCCTCCTG GAATGGCTAG TAAAATAAAC
    ACTAAAGCTT TGCAGTCCCC GAAAAGACCA CGAAGCCCCG GGAGTAATTC GAAAGTTCCT
    GAAATTGAGG TCACTGTGGA AGGCCCTAAT AATAGCAATC CTCAAACCTC AGCCGTTCGA
    ACCCCTACCC AGACGAATGG TTCTAATGTT CCCTTCAAGC CTCGAGGGAG AGAGTTTTCC
    TTTGAGGCCT GGAATGCCAA AATCACAGAC CTAAAACAGA AAGTTGAAAA TCTTTTCAAT
    GAAAAATGTG GTGAAGCTCT TGGCCTTAAA CAAGCTGTGA AAGTGCCGTT CGCGTTATTT
    GAGTCTTTCC CAGAAGACTT TTATGTGGAA GGCTTACCCG AGGGTGTGCC ATTCCGACGA
    CCGTCTACTT TTGGCATTCC AAGGCTGGAG AAGATACTCA GAAACAAAGC CAAAATTAAG
    TTCATCATTA AAAAGCCAGA AATGTTTGAG ACTGCAATTA AGGAGAGCAC CTCCTCATCT
    AAGAGCCCTC CAAGAAAAAT CAATTCATCA CCCAGTGTTA ATACTACTGC ATCAGGTGTT
    GAAGACCTTA ACATCATTCA GGTGACAATT CCAGACGATG ATAATGAAAG ACTATCAAAA
    GTTGAAAAAG CTCGACAGCT AAGAGAGCAA GTTAATGACC TCTTCAGTCG AAAATTTGGT
    GAAGCCATTG GGATGGGTTT CCCCGTGAAG GTTCCTTACA GGAAAATCAC AATTAACCCT
    GGCTGTGTGG TGGTTGATGG CATGCCCCCC GGAGTGTCAT TCAAAGCCCC CAGCTATCTG
    GAAATCAGCT CCATGAGAAG GATCTTAGAT TCTGCTGAGT TCATTAAATT CACAGTCATT
    AGACCATTTC CAGGACTTGT GATTAATAAC CAGTTGGTTG ATCAGAATGA GTCAGAAGGC
    CCAGTGATCC AAGAATCAGC TGAGCCAACC CAGTTGGAGG TTCCTGCAAC CGAAGAAATA
    AAGGAGACTG ATGGAAACTC CCAAATCAAG CAAGAACCAG ACCCTACGTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230343
    Clone ID Related Accession (Same CDS sequence) XM_005225178.4 , XM_024984996.1
    Accession Version XM_005225178.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2877bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225178.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATGATGACTA TCCTCCACCT
    GCCAAGAGAC CAAAGAGCAG TGAACCTCCC CAGCCACCCG TCACAGAACC TGCGAATGCC
    GGCAAACGGA AAGTGAGGGA GTTCAACTTC GAGAAATGGA ATGCTCGAAT TACTGATCTG
    CGTAAACAAG TTGAAGAGTT GTTTGAAAGA AAGTATGCTC AAGCTATAAA AGCCAAAGGT
    CCTGTGACGA TCCCGTACCC TCTTTTCCAG TCCCATGTGG AAGATCTTTA TGTAGAAGGA
    CTTCCAGAAG GAATCCCCTT TAGAAGGCCA TCAACTTATG GAATCCCTCG CCTTGAGAGG
    ATATTACTTG CAAAGGAAAG GATTCGTTTT GTGATTAAGA AGCATGAACT TTTGAATTCA
    ACACGTGAAG ACTTACAGCT TGATAAACCA GCTTCAGGAG TCAAGGAAGA GTGGTATGCC
    AGAATCACTA AGTTACGAAA GATGGTAGAT CAGCTTTTCT GTAAAAAATT TGCTGAAGCC
    TTAGGGAGCA CCGAAGCTAA GGCTGTACCT TACCAGAAAT TTGAAGCACA CCCTAATGAC
    CTCTACGTGG AAGGACTGCC AGAAAACATT CCTTTTAGAA GTCCCTCGTG GTATGGGATC
    CCAAGGCTGG AAAAGATTAT TCAAGTGGGC AATCGAATTA AATTTGTTAT TAAAAGACCA
    GAGCTTCTGA CGCACAGCAC TACTGAAGTT ACTCAGCCCA GAACGAATAC ACCAGTCAAA
    GAAGATTGGA ATGTCAGAAT TACCAAGCTA CGGAAGCAAG TGGAAGAGAT TTTTAATTTG
    AAATTTGCTC AAGCTCTTGG ACTCACAGAA GCAGTAAAAG TACCATACCC GGTGTTTGAA
    TCAAACCCCG AGTTCCTCTA TGTAGAAGGC TTGCCAGAAG GAATTCCCTT TCGAAGCCCT
    ACCTGGTTTG GAATTCCAAG ACTGGAAAGG ATTGTCCGTG GAAGTAATAA AATCAAGTTT
    GTTGTTAAAA AACCTGAACT AGTTATTTCC TACTTGCCTC CTGGAATGGC TAGTAAAATA
    AACACTAAAG CTTTGCAGTC CCCGAAAAGA CCACGAAGCC CCGGGAGTAA TTCGAAAGTT
    CCTGAAATTG AGGTCACTGT GGAAGGCCCT AATAATAGCA ATCCTCAAAC CTCAGCCGTT
    CGAACCCCTA CCCAGACGAA TGGTTCTAAT GTTCCCTTCA AGCCTCGAGG GAGAGAGTTT
    TCCTTTGAGG CCTGGAATGC CAAAATCACA GACCTAAAAC AGAAAGTTGA AAATCTTTTC
    AATGAAAAAT GTGGTGAAGC TCTTGGCCTT AAACAAGCTG TGAAAGTGCC GTTCGCGTTA
    TTTGAGTCTT TCCCAGAAGA CTTTTATGTG GAAGGCTTAC CCGAGGGTGT GCCATTCCGA
    CGACCGTCTA CTTTTGGCAT TCCAAGGCTG GAGAAGATAC TCAGAAACAA AGCCAAAATT
    AAGTTCATCA TTAAAAAGCC AGAAATGTTT GAGACTGCAA TTAAGGAGAG CACCTCCTCA
    TCTAAGAGCC CTCCAAGAAA AATCAATTCA TCACCCAGTG TTAATACTAC TGCATCAGGT
    GTTGAAGACC TTAACATCAT TCAGGTGACA ATTCCAGACG ATGATAATGA AAGACTATCA
    AAAGTTGAAA AAGCTCGACA GCTAAGAGAG CAAGTTAATG ACCTCTTCAG TCGAAAATTT
    GGTGAAGCCA TTGGGATGGG TTTCCCCGTG AAGGTTCCTT ACAGGAAAAT CACAATTAAC
    CCTGGCTGTG TGGTGGTTGA TGGCATGCCC CCCGGAGTGT CATTCAAAGC CCCCAGCTAT
    CTGGAAATCA GCTCCATGAG AAGGATCTTA GATTCTGCTG AGTTCATTAA ATTCACAGTC
    ATTAGACCAT TTCCAGGACT TGTGATTAAT AACCAGTTGG TTGATCAGAA TGAGTCAGAA
    GGCCCAGTGA TCCAAGAATC AGCTGAGCCA ACCCAGTTGG AGGTTCCTGC AACCGAAGAA
    ATAAAGGAGA CTGATGGAAA CTCCCAAATC AAGCAAGAAC CAGACCCTAC GTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225235.1
    CDS414..3290
    Translation

    Target ORF information:

    RefSeq Version XM_005225178.4
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225178.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATGATGACTA TCCTCCACCT
    GCCAAGAGAC CAAAGAGCAG TGAACCTCCC CAGCCACCCG TCACAGAACC TGCGAATGCC
    GGCAAACGGA AAGTGAGGGA GTTCAACTTC GAGAAATGGA ATGCTCGAAT TACTGATCTG
    CGTAAACAAG TTGAAGAGTT GTTTGAAAGA AAGTATGCTC AAGCTATAAA AGCCAAAGGT
    CCTGTGACGA TCCCGTACCC TCTTTTCCAG TCCCATGTGG AAGATCTTTA TGTAGAAGGA
    CTTCCAGAAG GAATCCCCTT TAGAAGGCCA TCAACTTATG GAATCCCTCG CCTTGAGAGG
    ATATTACTTG CAAAGGAAAG GATTCGTTTT GTGATTAAGA AGCATGAACT TTTGAATTCA
    ACACGTGAAG ACTTACAGCT TGATAAACCA GCTTCAGGAG TCAAGGAAGA GTGGTATGCC
    AGAATCACTA AGTTACGAAA GATGGTAGAT CAGCTTTTCT GTAAAAAATT TGCTGAAGCC
    TTAGGGAGCA CCGAAGCTAA GGCTGTACCT TACCAGAAAT TTGAAGCACA CCCTAATGAC
    CTCTACGTGG AAGGACTGCC AGAAAACATT CCTTTTAGAA GTCCCTCGTG GTATGGGATC
    CCAAGGCTGG AAAAGATTAT TCAAGTGGGC AATCGAATTA AATTTGTTAT TAAAAGACCA
    GAGCTTCTGA CGCACAGCAC TACTGAAGTT ACTCAGCCCA GAACGAATAC ACCAGTCAAA
    GAAGATTGGA ATGTCAGAAT TACCAAGCTA CGGAAGCAAG TGGAAGAGAT TTTTAATTTG
    AAATTTGCTC AAGCTCTTGG ACTCACAGAA GCAGTAAAAG TACCATACCC GGTGTTTGAA
    TCAAACCCCG AGTTCCTCTA TGTAGAAGGC TTGCCAGAAG GAATTCCCTT TCGAAGCCCT
    ACCTGGTTTG GAATTCCAAG ACTGGAAAGG ATTGTCCGTG GAAGTAATAA AATCAAGTTT
    GTTGTTAAAA AACCTGAACT AGTTATTTCC TACTTGCCTC CTGGAATGGC TAGTAAAATA
    AACACTAAAG CTTTGCAGTC CCCGAAAAGA CCACGAAGCC CCGGGAGTAA TTCGAAAGTT
    CCTGAAATTG AGGTCACTGT GGAAGGCCCT AATAATAGCA ATCCTCAAAC CTCAGCCGTT
    CGAACCCCTA CCCAGACGAA TGGTTCTAAT GTTCCCTTCA AGCCTCGAGG GAGAGAGTTT
    TCCTTTGAGG CCTGGAATGC CAAAATCACA GACCTAAAAC AGAAAGTTGA AAATCTTTTC
    AATGAAAAAT GTGGTGAAGC TCTTGGCCTT AAACAAGCTG TGAAAGTGCC GTTCGCGTTA
    TTTGAGTCTT TCCCAGAAGA CTTTTATGTG GAAGGCTTAC CCGAGGGTGT GCCATTCCGA
    CGACCGTCTA CTTTTGGCAT TCCAAGGCTG GAGAAGATAC TCAGAAACAA AGCCAAAATT
    AAGTTCATCA TTAAAAAGCC AGAAATGTTT GAGACTGCAA TTAAGGAGAG CACCTCCTCA
    TCTAAGAGCC CTCCAAGAAA AATCAATTCA TCACCCAGTG TTAATACTAC TGCATCAGGT
    GTTGAAGACC TTAACATCAT TCAGGTGACA ATTCCAGACG ATGATAATGA AAGACTATCA
    AAAGTTGAAA AAGCTCGACA GCTAAGAGAG CAAGTTAATG ACCTCTTCAG TCGAAAATTT
    GGTGAAGCCA TTGGGATGGG TTTCCCCGTG AAGGTTCCTT ACAGGAAAAT CACAATTAAC
    CCTGGCTGTG TGGTGGTTGA TGGCATGCCC CCCGGAGTGT CATTCAAAGC CCCCAGCTAT
    CTGGAAATCA GCTCCATGAG AAGGATCTTA GATTCTGCTG AGTTCATTAA ATTCACAGTC
    ATTAGACCAT TTCCAGGACT TGTGATTAAT AACCAGTTGG TTGATCAGAA TGAGTCAGAA
    GGCCCAGTGA TCCAAGAATC AGCTGAGCCA ACCCAGTTGG AGGTTCCTGC AACCGAAGAA
    ATAAAGGAGA CTGATGGAAA CTCCCAAATC AAGCAAGAAC CAGACCCTAC GTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230343
    Clone ID Related Accession (Same CDS sequence) XM_005225178.4 , XM_024984996.1
    Accession Version XM_024984996.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2877bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product general transcription factor II-I isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATGATGACTA TCCTCCACCT
    GCCAAGAGAC CAAAGAGCAG TGAACCTCCC CAGCCACCCG TCACAGAACC TGCGAATGCC
    GGCAAACGGA AAGTGAGGGA GTTCAACTTC GAGAAATGGA ATGCTCGAAT TACTGATCTG
    CGTAAACAAG TTGAAGAGTT GTTTGAAAGA AAGTATGCTC AAGCTATAAA AGCCAAAGGT
    CCTGTGACGA TCCCGTACCC TCTTTTCCAG TCCCATGTGG AAGATCTTTA TGTAGAAGGA
    CTTCCAGAAG GAATCCCCTT TAGAAGGCCA TCAACTTATG GAATCCCTCG CCTTGAGAGG
    ATATTACTTG CAAAGGAAAG GATTCGTTTT GTGATTAAGA AGCATGAACT TTTGAATTCA
    ACACGTGAAG ACTTACAGCT TGATAAACCA GCTTCAGGAG TCAAGGAAGA GTGGTATGCC
    AGAATCACTA AGTTACGAAA GATGGTAGAT CAGCTTTTCT GTAAAAAATT TGCTGAAGCC
    TTAGGGAGCA CCGAAGCTAA GGCTGTACCT TACCAGAAAT TTGAAGCACA CCCTAATGAC
    CTCTACGTGG AAGGACTGCC AGAAAACATT CCTTTTAGAA GTCCCTCGTG GTATGGGATC
    CCAAGGCTGG AAAAGATTAT TCAAGTGGGC AATCGAATTA AATTTGTTAT TAAAAGACCA
    GAGCTTCTGA CGCACAGCAC TACTGAAGTT ACTCAGCCCA GAACGAATAC ACCAGTCAAA
    GAAGATTGGA ATGTCAGAAT TACCAAGCTA CGGAAGCAAG TGGAAGAGAT TTTTAATTTG
    AAATTTGCTC AAGCTCTTGG ACTCACAGAA GCAGTAAAAG TACCATACCC GGTGTTTGAA
    TCAAACCCCG AGTTCCTCTA TGTAGAAGGC TTGCCAGAAG GAATTCCCTT TCGAAGCCCT
    ACCTGGTTTG GAATTCCAAG ACTGGAAAGG ATTGTCCGTG GAAGTAATAA AATCAAGTTT
    GTTGTTAAAA AACCTGAACT AGTTATTTCC TACTTGCCTC CTGGAATGGC TAGTAAAATA
    AACACTAAAG CTTTGCAGTC CCCGAAAAGA CCACGAAGCC CCGGGAGTAA TTCGAAAGTT
    CCTGAAATTG AGGTCACTGT GGAAGGCCCT AATAATAGCA ATCCTCAAAC CTCAGCCGTT
    CGAACCCCTA CCCAGACGAA TGGTTCTAAT GTTCCCTTCA AGCCTCGAGG GAGAGAGTTT
    TCCTTTGAGG CCTGGAATGC CAAAATCACA GACCTAAAAC AGAAAGTTGA AAATCTTTTC
    AATGAAAAAT GTGGTGAAGC TCTTGGCCTT AAACAAGCTG TGAAAGTGCC GTTCGCGTTA
    TTTGAGTCTT TCCCAGAAGA CTTTTATGTG GAAGGCTTAC CCGAGGGTGT GCCATTCCGA
    CGACCGTCTA CTTTTGGCAT TCCAAGGCTG GAGAAGATAC TCAGAAACAA AGCCAAAATT
    AAGTTCATCA TTAAAAAGCC AGAAATGTTT GAGACTGCAA TTAAGGAGAG CACCTCCTCA
    TCTAAGAGCC CTCCAAGAAA AATCAATTCA TCACCCAGTG TTAATACTAC TGCATCAGGT
    GTTGAAGACC TTAACATCAT TCAGGTGACA ATTCCAGACG ATGATAATGA AAGACTATCA
    AAAGTTGAAA AAGCTCGACA GCTAAGAGAG CAAGTTAATG ACCTCTTCAG TCGAAAATTT
    GGTGAAGCCA TTGGGATGGG TTTCCCCGTG AAGGTTCCTT ACAGGAAAAT CACAATTAAC
    CCTGGCTGTG TGGTGGTTGA TGGCATGCCC CCCGGAGTGT CATTCAAAGC CCCCAGCTAT
    CTGGAAATCA GCTCCATGAG AAGGATCTTA GATTCTGCTG AGTTCATTAA ATTCACAGTC
    ATTAGACCAT TTCCAGGACT TGTGATTAAT AACCAGTTGG TTGATCAGAA TGAGTCAGAA
    GGCCCAGTGA TCCAAGAATC AGCTGAGCCA ACCCAGTTGG AGGTTCCTGC AACCGAAGAA
    ATAAAGGAGA CTGATGGAAA CTCCCAAATC AAGCAAGAAC CAGACCCTAC GTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840764.1
    CDS461..3337
    Translation

    Target ORF information:

    RefSeq Version XM_024984996.1
    Organism Bos taurus(cattle)
    Definition Bos taurus general transcription factor IIi (GTF2I), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984996.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCCCAAG TAGCAGTGTC TACCCTGCCT GTCGAAGACG AGGAGTCCTC AGAGAGCAGG 
    ATGGTGGTGA CGTTCCTCAT GTCTGCTCTT GAGTCCATGT GTAAAGAACT GGCCAAGTCC
    AAGGCGGAAG TAGCGTGCAT CGCAGTGTAT GAGACAGACG TGTTTGTTGT TGGAACCGAG
    AGAGGACGTG CTTTTGTCAA CACTAGAAAG GACTTTCAGA AAGATTTTGT AAAATACTGT
    GTTGAAGAAG AAGAAAAGGC TGCAGAGATG CATAAAATGA AATCTACAGC CCAGGCAAAT
    CGGATGAGTG TCGATGCTGT GGAGATAGAA ACACTCAGAA AAACAGTTGA GGACTATTTC
    TGCTTTTGCT ATGGGAAAGC TTTAGGCAAG TCAACAGTGG TGCCTGTTCC ATATGAAAAG
    ATGCTCCGAG ATCAGTCGGC CGTGGTGGTG CAGGGGCTTC CAGAAGGAGT AGCCTTCAAA
    CACCCTGAAA ACTATGATCT TGCAACCCTG AAGTGGATTT TGGAGAACAA AGCAGGGATT
    TCATTTATCA TTAAAAGACC TTTCCTAGAG CCAAAGAAGC ACCTAGGTGG TCGTGTGATG
    GTTACAGATG CTGAAAGGTC AATGATATCT CCAAGTGGAA GCTGTGGCCC CGTCAAAGTA
    AAAACAGAAC CCTCAGAGGA TTCTGGCATT TCTCTGGAAA TGGCAGCTGT GACAGTGAAG
    GAAGAATCTG AAGATCCTGA TTACTATCAA TATAACATTC AAGGAAGCCA TCATTCTTCA
    GAGGGCAATG AAGGAACAGA AATGGAAGTA CCAGCAGAAG ATGATGACTA TCCTCCACCT
    GCCAAGAGAC CAAAGAGCAG TGAACCTCCC CAGCCACCCG TCACAGAACC TGCGAATGCC
    GGCAAACGGA AAGTGAGGGA GTTCAACTTC GAGAAATGGA ATGCTCGAAT TACTGATCTG
    CGTAAACAAG TTGAAGAGTT GTTTGAAAGA AAGTATGCTC AAGCTATAAA AGCCAAAGGT
    CCTGTGACGA TCCCGTACCC TCTTTTCCAG TCCCATGTGG AAGATCTTTA TGTAGAAGGA
    CTTCCAGAAG GAATCCCCTT TAGAAGGCCA TCAACTTATG GAATCCCTCG CCTTGAGAGG
    ATATTACTTG CAAAGGAAAG GATTCGTTTT GTGATTAAGA AGCATGAACT TTTGAATTCA
    ACACGTGAAG ACTTACAGCT TGATAAACCA GCTTCAGGAG TCAAGGAAGA GTGGTATGCC
    AGAATCACTA AGTTACGAAA GATGGTAGAT CAGCTTTTCT GTAAAAAATT TGCTGAAGCC
    TTAGGGAGCA CCGAAGCTAA GGCTGTACCT TACCAGAAAT TTGAAGCACA CCCTAATGAC
    CTCTACGTGG AAGGACTGCC AGAAAACATT CCTTTTAGAA GTCCCTCGTG GTATGGGATC
    CCAAGGCTGG AAAAGATTAT TCAAGTGGGC AATCGAATTA AATTTGTTAT TAAAAGACCA
    GAGCTTCTGA CGCACAGCAC TACTGAAGTT ACTCAGCCCA GAACGAATAC ACCAGTCAAA
    GAAGATTGGA ATGTCAGAAT TACCAAGCTA CGGAAGCAAG TGGAAGAGAT TTTTAATTTG
    AAATTTGCTC AAGCTCTTGG ACTCACAGAA GCAGTAAAAG TACCATACCC GGTGTTTGAA
    TCAAACCCCG AGTTCCTCTA TGTAGAAGGC TTGCCAGAAG GAATTCCCTT TCGAAGCCCT
    ACCTGGTTTG GAATTCCAAG ACTGGAAAGG ATTGTCCGTG GAAGTAATAA AATCAAGTTT
    GTTGTTAAAA AACCTGAACT AGTTATTTCC TACTTGCCTC CTGGAATGGC TAGTAAAATA
    AACACTAAAG CTTTGCAGTC CCCGAAAAGA CCACGAAGCC CCGGGAGTAA TTCGAAAGTT
    CCTGAAATTG AGGTCACTGT GGAAGGCCCT AATAATAGCA ATCCTCAAAC CTCAGCCGTT
    CGAACCCCTA CCCAGACGAA TGGTTCTAAT GTTCCCTTCA AGCCTCGAGG GAGAGAGTTT
    TCCTTTGAGG CCTGGAATGC CAAAATCACA GACCTAAAAC AGAAAGTTGA AAATCTTTTC
    AATGAAAAAT GTGGTGAAGC TCTTGGCCTT AAACAAGCTG TGAAAGTGCC GTTCGCGTTA
    TTTGAGTCTT TCCCAGAAGA CTTTTATGTG GAAGGCTTAC CCGAGGGTGT GCCATTCCGA
    CGACCGTCTA CTTTTGGCAT TCCAAGGCTG GAGAAGATAC TCAGAAACAA AGCCAAAATT
    AAGTTCATCA TTAAAAAGCC AGAAATGTTT GAGACTGCAA TTAAGGAGAG CACCTCCTCA
    TCTAAGAGCC CTCCAAGAAA AATCAATTCA TCACCCAGTG TTAATACTAC TGCATCAGGT
    GTTGAAGACC TTAACATCAT TCAGGTGACA ATTCCAGACG ATGATAATGA AAGACTATCA
    AAAGTTGAAA AAGCTCGACA GCTAAGAGAG CAAGTTAATG ACCTCTTCAG TCGAAAATTT
    GGTGAAGCCA TTGGGATGGG TTTCCCCGTG AAGGTTCCTT ACAGGAAAAT CACAATTAAC
    CCTGGCTGTG TGGTGGTTGA TGGCATGCCC CCCGGAGTGT CATTCAAAGC CCCCAGCTAT
    CTGGAAATCA GCTCCATGAG AAGGATCTTA GATTCTGCTG AGTTCATTAA ATTCACAGTC
    ATTAGACCAT TTCCAGGACT TGTGATTAAT AACCAGTTGG TTGATCAGAA TGAGTCAGAA
    GGCCCAGTGA TCCAAGAATC AGCTGAGCCA ACCCAGTTGG AGGTTCCTGC AACCGAAGAA
    ATAAAGGAGA CTGATGGAAA CTCCCAAATC AAGCAAGAAC CAGACCCTAC GTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.