TBC1D4 cDNA ORF clone, Bos taurus(cattle)

The following TBC1D4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBC1D4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa221162 XM_024999916.1
Latest version!
Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa239239 NM_001206310.3
Latest version!
Bos taurus TBC1 domain family member 4 (TBC1D4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221158 XM_005213844.4
Latest version!
Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221159 XM_024999914.1
Latest version!
Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221160 XM_005213847.4
Latest version!
Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221161 XM_024999915.1
Latest version!
Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa52893 XM_010810848.2
Latest version!
Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa216390 NM_001206310.2
Latest version!
Bos taurus TBC1 domain family member 4 (TBC1D4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa40613 NM_001206310.1
Latest version!
Bos taurus TBC1 domain family, member 4 (TBC1D4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa24805 XM_005213844.3
Latest version!
Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa24808 XM_005213847.3
Latest version!
Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52891 XM_010810846.2
Latest version!
Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa221162
    Clone ID Related Accession (Same CDS sequence) XM_024999916.1
    Accession Version XM_024999916.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGGAAAT CTCCCTCAGC GATGCAGCAG GATGGCCTGG ACAGGAGTGA GCTGCTGCCA 
    CTGTCCCCTC TTGCTCCAAC CATGGAGGAG GAACCTCTAA TGATATTCAT GTCTGATGAA
    GATGACCCAG AAAAGGGTGA AGAGAGAAAC AAGTCGGAGG AACTGAGGTG TTTGTGGAGA
    AAAGCTATAC ACCAGCAGAT CCTGTTGCTT CGAATGGAAA AAGAAAACCA GAAACTTGAA
    GCAAGCAGAG ATGAACTGCA GTCCAGAAAA GTTAAATTGG ATTATGAAGA AGTTGGTGCA
    TGTCAGAAGG AGGTCTTAAC AATCTGGGAT AAGAAGTTGT TAAACTGCAG AGCTAAAATC
    AGATGCGATA TGGAAGATAT TCATACTTCT CTTAAAGAAG GTGTTCCCAA AAGTCGACGA
    GGAGAAATCT GGCAGTTCCT AGCTTTGCAA TATCGTCTGA GACACAGATT GCCTAATAAA
    CAACAACCTC CCGACATATC CTATAAAGAG CTTTTAAAAC AGCTCACGGC TCAGCAGCAT
    GCTATCCTCG TGGATTTAGG GAGAACATTT CCTACTCATC CTTACTTTTC AGCACAGCTT
    GGGGCAGGGC AGCTGTCGCT CTTTAATCTC TTGAAAGCCT ACTCATTACT GGACAAAGAA
    GTGGGTTACT GTCAGGGGAT CAGCTTTGTG GCTGGAGTCT TGCTTCTACA CATGAGTGAA
    GAGCAAGCCT TTGAAATGCT GAAATTCCTC ATGTATGACC TGGGCTTCCG CAAGCAGTAC
    CGACCTGACA TGATGTCGCT GCAGATTCAA ATGTACCAGC TGTCCAGGCT CCTTCATGAT
    TATCACAGAG ATCTCTACAA TCATCTTGAA GAAAATGAAA TCAGCCCCAG TCTTTATGCT
    GCCCCCTGGT TCCTCACGTT GTTTGCCTCT CAATTTCCAT TAGGATTTGT AGCCAGAGTG
    TTTGATATTA TTTTTCTTCA GGGAACTGAA GTTATATTTA AGGTTGCCCT AAGCCTGTTG
    AGCAACCAAG AGACACTCAT AATGGAATGT GAAAACTTTG AAAATATTGT TGAATTCCTT
    AAAATCACTC TCCCCGATAT GAATTCATCT GAAATGGAAA AAATAATCAC CCAGGTTTTT
    GAGATGGATA TTTCTAAACA GTTACATGCC TATGAGGTGG AGTATCACGT GCTACAGGAT
    GAACTCCAAG AATCTTCCTA TGCTTGCGAG GACAGTGAGC CCATAGAGAA GCTGGAGAGG
    GCCAACAGCC AACTGAAAAG ACAAAACATG GACCTTCTAG AAAAATTACA GATAGCTCAT
    GCTAAAATTC AGACCTTGGA GTCCAACCTG GAAAATCTTT TAACCAGAGA GACCAAAATG
    AAGTCTTTGA TCCGGACCCT GGAACAGGAG AAGACGGCTT ATCAGAAGGC AGTGGAGCAG
    ATCTGGAAGC TGCTGCCTGC AGATATTTTA GCCAACTGCG AACCACTGCT GAGAGACTTA
    AACTGCAACC CTAACAACAA AGCCAAGACA GGAAACAAGC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855684.1
    CDS653..2197
    Translation

    Target ORF information:

    RefSeq Version XM_024999916.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999916.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGGAAAT CTCCCTCAGC GATGCAGCAG GATGGCCTGG ACAGGAGTGA GCTGCTGCCA 
    CTGTCCCCTC TTGCTCCAAC CATGGAGGAG GAACCTCTAA TGATATTCAT GTCTGATGAA
    GATGACCCAG AAAAGGGTGA AGAGAGAAAC AAGTCGGAGG AACTGAGGTG TTTGTGGAGA
    AAAGCTATAC ACCAGCAGAT CCTGTTGCTT CGAATGGAAA AAGAAAACCA GAAACTTGAA
    GCAAGCAGAG ATGAACTGCA GTCCAGAAAA GTTAAATTGG ATTATGAAGA AGTTGGTGCA
    TGTCAGAAGG AGGTCTTAAC AATCTGGGAT AAGAAGTTGT TAAACTGCAG AGCTAAAATC
    AGATGCGATA TGGAAGATAT TCATACTTCT CTTAAAGAAG GTGTTCCCAA AAGTCGACGA
    GGAGAAATCT GGCAGTTCCT AGCTTTGCAA TATCGTCTGA GACACAGATT GCCTAATAAA
    CAACAACCTC CCGACATATC CTATAAAGAG CTTTTAAAAC AGCTCACGGC TCAGCAGCAT
    GCTATCCTCG TGGATTTAGG GAGAACATTT CCTACTCATC CTTACTTTTC AGCACAGCTT
    GGGGCAGGGC AGCTGTCGCT CTTTAATCTC TTGAAAGCCT ACTCATTACT GGACAAAGAA
    GTGGGTTACT GTCAGGGGAT CAGCTTTGTG GCTGGAGTCT TGCTTCTACA CATGAGTGAA
    GAGCAAGCCT TTGAAATGCT GAAATTCCTC ATGTATGACC TGGGCTTCCG CAAGCAGTAC
    CGACCTGACA TGATGTCGCT GCAGATTCAA ATGTACCAGC TGTCCAGGCT CCTTCATGAT
    TATCACAGAG ATCTCTACAA TCATCTTGAA GAAAATGAAA TCAGCCCCAG TCTTTATGCT
    GCCCCCTGGT TCCTCACGTT GTTTGCCTCT CAATTTCCAT TAGGATTTGT AGCCAGAGTG
    TTTGATATTA TTTTTCTTCA GGGAACTGAA GTTATATTTA AGGTTGCCCT AAGCCTGTTG
    AGCAACCAAG AGACACTCAT AATGGAATGT GAAAACTTTG AAAATATTGT TGAATTCCTT
    AAAATCACTC TCCCCGATAT GAATTCATCT GAAATGGAAA AAATAATCAC CCAGGTTTTT
    GAGATGGATA TTTCTAAACA GTTACATGCC TATGAGGTGG AGTATCACGT GCTACAGGAT
    GAACTCCAAG AATCTTCCTA TGCTTGCGAG GACAGTGAGC CCATAGAGAA GCTGGAGAGG
    GCCAACAGCC AACTGAAAAG ACAAAACATG GACCTTCTAG AAAAATTACA GATAGCTCAT
    GCTAAAATTC AGACCTTGGA GTCCAACCTG GAAAATCTTT TAACCAGAGA GACCAAAATG
    AAGTCTTTGA TCCGGACCCT GGAACAGGAG AAGACGGCTT ATCAGAAGGC AGTGGAGCAG
    ATCTGGAAGC TGCTGCCTGC AGATATTTTA GCCAACTGCG AACCACTGCT GAGAGACTTA
    AACTGCAACC CTAACAACAA AGCCAAGACA GGAAACAAGC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa239239
    Clone ID Related Accession (Same CDS sequence) NM_001206310.3
    Accession Version NM_001206310.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-19
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000012.1. On Dec 20, 2018 this sequence version replaced NM_001206310.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAATGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGACT
    ATTTCAAATA GTACAATCCC GGAAAATGCG ACAAGCAGTG GAAGGTTCAA ACTTGACATT
    CTGAAAAATA AAGCTAAGAG ATCCTTAACT AGCTCCCTGG AAAATATCTT CTCAAGGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC GTTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGC
    ATCCTTCCAC CAGCTCGGCG CATGCACAAG GAGTGTAATT CTTCCTCCAG TCTTCCAAGT
    CTTCACACTT CCTTCTCTGC CCCTTCCTTC ACTGCCCCCC CTTTCTTGAA AAGCTTTTAC
    CAGAATTCAG GTAGACTATC CCCACAGTAT GAGAATGAAA TCAGACAAGA CACTGCTTCA
    GAATCAAGTG ATGGAGAGGG GAGAAAAAGG ACCTCGTCCA CCTGCAGCAA TGAATCCCTA
    AACGCTGGAG GAACCCCCGT CACTCCTCGC CGAGTTTCCT GGCGGCAGCG CATCTTCCTC
    AGGGTTGCTT CGCCCATGAG GAAATCTCCC TCAGCGATGC AGCAGGATGG CCTGGACAGG
    AGTGAGCTGC TGCCACTGTC CCCTCTTGCT CCAACCATGG AGGAGGAACC TCTAATGATA
    TTCATGTCTG ATGAAGATGA CCCAGAAAAG GGTGAAGAGA GAAACAAGTC GGAGGAACTG
    AGGTGTTTGT GGAGAAAAGC TATACACCAG CAGATCCTGT TGCTTCGAAT GGAAAAAGAA
    AACCAGAAAC TTGAAGCAAG CAGAGATGAA CTGCAGTCCA GAAAAGTTAA ATTGGATTAT
    GAAGAAGTTG GTGCATGTCA GAAGGAGGTC TTAACAATCT GGGATAAGAA GTTGTTAAAC
    TGCAGAGCTA AAATCAGATG CGATATGGAA GATATTCATA CTTCTCTTAA AGAAGGTGTT
    CCCAAAAGTC GACGAGGAGA AATCTGGCAG TTCCTAGCTT TGCAATATCG TCTGAGACAC
    AGATTGCCTA ATAAACAACA ACCTCCCGAC ATATCCTATA AAGAGCTTTT AAAACAGCTC
    ACGGCTCAGC AGCATGCTAT CCTCGTGGAT TTAGGGAGAA CATTTCCTAC TCATCCTTAC
    TTTTCAGCAC AGCTTGGGGC AGGGCAGCTG TCGCTCTTTA ATCTCTTGAA AGCCTACTCA
    TTACTGGACA AAGAAGTGGG TTACTGTCAG GGGATCAGCT TTGTGGCTGG AGTCTTGCTT
    CTACACATGA GTGAAGAGCA AGCCTTTGAA ATGCTGAAAT TCCTCATGTA TGACCTGGGC
    TTCCGCAAGC AGTACCGACC TGACATGATG TCGCTGCAGA TTCAAATGTA CCAGCTGTCC
    AGGCTCCTTC ATGATTATCA CAGAGATCTC TACAATCATC TTGAAGAAAA TGAAATCAGC
    CCCAGTCTTT ATGCTGCCCC CTGGTTCCTC ACGTTGTTTG CCTCTCAATT TCCATTAGGA
    TTTGTAGCCA GAGTGTTTGA TATTATTTTT CTTCAGGGAA CTGAAGTTAT ATTTAAGGTT
    GCCCTAAGCC TGTTGAGCAA CCAAGAGACA CTCATAATGG AATGTGAAAA CTTTGAAAAT
    ATTGTTGAAT TCCTTAAAAT CACTCTCCCC GATATGAATT CATCTGAAAT GGAAAAAATA
    ATCACCCAGG TTTTTGAGAT GGATATTTCT AAACAGTTAC ATGCCTATGA GGTGGAGTAT
    CACGTGCTAC AGGATGAACT CCAAGAATCT TCCTATGCTT GCGAGGACAG TGAGCCCATA
    GAGAAGCTGG AGAGGGCCAA CAGCCAACTG AAAAGACAAA ACATGGACCT TCTAGAAAAA
    TTACAGATAG CTCATGCTAA AATTCAGACC TTGGAGTCCA ACCTGGAAAA TCTTTTAACC
    AGAGAGACCA AAATGAAGTC TTTGATCCGG ACCCTGGAAC AGGAGAAGAC GGCTTATCAG
    AAGGCAGTGG AGCAGATCTG GAAGCTGCTG CCTGCAGATA TTTTAGCCAA CTGCGAACCA
    CTGCTGAGAG ACTTAAACTG CAACCCTAAC AACAAAGCCA AGACAGGAAA CAAGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193239.1
    CDS1..3900
    Translation

    Target ORF information:

    RefSeq Version NM_001206310.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 4 (TBC1D4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206310.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAATGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGACT
    ATTTCAAATA GTACAATCCC GGAAAATGCG ACAAGCAGTG GAAGGTTCAA ACTTGACATT
    CTGAAAAATA AAGCTAAGAG ATCCTTAACT AGCTCCCTGG AAAATATCTT CTCAAGGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC GTTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGC
    ATCCTTCCAC CAGCTCGGCG CATGCACAAG GAGTGTAATT CTTCCTCCAG TCTTCCAAGT
    CTTCACACTT CCTTCTCTGC CCCTTCCTTC ACTGCCCCCC CTTTCTTGAA AAGCTTTTAC
    CAGAATTCAG GTAGACTATC CCCACAGTAT GAGAATGAAA TCAGACAAGA CACTGCTTCA
    GAATCAAGTG ATGGAGAGGG GAGAAAAAGG ACCTCGTCCA CCTGCAGCAA TGAATCCCTA
    AACGCTGGAG GAACCCCCGT CACTCCTCGC CGAGTTTCCT GGCGGCAGCG CATCTTCCTC
    AGGGTTGCTT CGCCCATGAG GAAATCTCCC TCAGCGATGC AGCAGGATGG CCTGGACAGG
    AGTGAGCTGC TGCCACTGTC CCCTCTTGCT CCAACCATGG AGGAGGAACC TCTAATGATA
    TTCATGTCTG ATGAAGATGA CCCAGAAAAG GGTGAAGAGA GAAACAAGTC GGAGGAACTG
    AGGTGTTTGT GGAGAAAAGC TATACACCAG CAGATCCTGT TGCTTCGAAT GGAAAAAGAA
    AACCAGAAAC TTGAAGCAAG CAGAGATGAA CTGCAGTCCA GAAAAGTTAA ATTGGATTAT
    GAAGAAGTTG GTGCATGTCA GAAGGAGGTC TTAACAATCT GGGATAAGAA GTTGTTAAAC
    TGCAGAGCTA AAATCAGATG CGATATGGAA GATATTCATA CTTCTCTTAA AGAAGGTGTT
    CCCAAAAGTC GACGAGGAGA AATCTGGCAG TTCCTAGCTT TGCAATATCG TCTGAGACAC
    AGATTGCCTA ATAAACAACA ACCTCCCGAC ATATCCTATA AAGAGCTTTT AAAACAGCTC
    ACGGCTCAGC AGCATGCTAT CCTCGTGGAT TTAGGGAGAA CATTTCCTAC TCATCCTTAC
    TTTTCAGCAC AGCTTGGGGC AGGGCAGCTG TCGCTCTTTA ATCTCTTGAA AGCCTACTCA
    TTACTGGACA AAGAAGTGGG TTACTGTCAG GGGATCAGCT TTGTGGCTGG AGTCTTGCTT
    CTACACATGA GTGAAGAGCA AGCCTTTGAA ATGCTGAAAT TCCTCATGTA TGACCTGGGC
    TTCCGCAAGC AGTACCGACC TGACATGATG TCGCTGCAGA TTCAAATGTA CCAGCTGTCC
    AGGCTCCTTC ATGATTATCA CAGAGATCTC TACAATCATC TTGAAGAAAA TGAAATCAGC
    CCCAGTCTTT ATGCTGCCCC CTGGTTCCTC ACGTTGTTTG CCTCTCAATT TCCATTAGGA
    TTTGTAGCCA GAGTGTTTGA TATTATTTTT CTTCAGGGAA CTGAAGTTAT ATTTAAGGTT
    GCCCTAAGCC TGTTGAGCAA CCAAGAGACA CTCATAATGG AATGTGAAAA CTTTGAAAAT
    ATTGTTGAAT TCCTTAAAAT CACTCTCCCC GATATGAATT CATCTGAAAT GGAAAAAATA
    ATCACCCAGG TTTTTGAGAT GGATATTTCT AAACAGTTAC ATGCCTATGA GGTGGAGTAT
    CACGTGCTAC AGGATGAACT CCAAGAATCT TCCTATGCTT GCGAGGACAG TGAGCCCATA
    GAGAAGCTGG AGAGGGCCAA CAGCCAACTG AAAAGACAAA ACATGGACCT TCTAGAAAAA
    TTACAGATAG CTCATGCTAA AATTCAGACC TTGGAGTCCA ACCTGGAAAA TCTTTTAACC
    AGAGAGACCA AAATGAAGTC TTTGATCCGG ACCCTGGAAC AGGAGAAGAC GGCTTATCAG
    AAGGCAGTGG AGCAGATCTG GAAGCTGCTG CCTGCAGATA TTTTAGCCAA CTGCGAACCA
    CTGCTGAGAG ACTTAAACTG CAACCCTAAC AACAAAGCCA AGACAGGAAA CAAGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221158
    Clone ID Related Accession (Same CDS sequence) XM_005213844.4
    Accession Version XM_005213844.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213844.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAATGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGACT
    ATTTCAAATA GTACAATCCC GGAAAATGCG ACAAGCAGTG GAAGGTTCAA ACTTGACATT
    CTGAAAAATA AAGCTAAGAG ATCCTTAACT AGCTCCCTGG AAAATATCTT CTCAAGGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC GTTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGC
    ATCCTTCCAC CAGCTCGGCG CATGCACAAG GAGTGTAATT CTTCCTCCAG TCTTCCAAGT
    CTTCACACTT CCTTCTCTGC CCCTTCCTTC ACTGCCCCCC CTTTCTTGAA AAGCTTTTAC
    CAGAATTCAG GTAGACTATC CCCACAGTAT GAGAATGAAA TCAGTGATGG AGAGGGGAGA
    AAAAGGACCT CGTCCACCTG CAGCAATGAA TCCCTAAACG CTGGAGGAAC CCCCGTCACT
    CCTCGCCGAG TTTCCTGGCG GCAGCGCATC TTCCTCAGGG TTGCTTCGCC CATGAGGAAA
    TCTCCCTCAG CGATGCAGCA GGATGGCCTG GACAGGAGTG AGCTGCTGCC ACTGTCCCCT
    CTTGCTCCAA CCATGGAGGA GGAACCTCTA ATGATATTCA TGTCTGATGA AGATGACCCA
    GAAAAGGGTG AAGAGAGAAA CAAGTCGGAG GAACTGAGGT GTTTGTGGAG AAAAGCTATA
    CACCAGCAGA TCCTGTTGCT TCGAATGGAA AAAGAAAACC AGAAACTTGA AGCAAGCAGA
    GATGAACTGC AGTCCAGAAA AGTTAAATTG GATTATGAAG AAGTTGGTGC ATGTCAGAAG
    GAGGTCTTAA CAATCTGGGA TAAGAAGTTG TTAAACTGCA GAGCTAAAAT CAGATGCGAT
    ATGGAAGATA TTCATACTTC TCTTAAAGAA GGTGTTCCCA AAAGTCGACG AGGAGAAATC
    TGGCAGTTCC TAGCTTTGCA ATATCGTCTG AGACACAGAT TGCCTAATAA ACAACAACCT
    CCCGACATAT CCTATAAAGA GCTTTTAAAA CAGCTCACGG CTCAGCAGCA TGCTATCCTC
    GTGGATTTAG GGAGAACATT TCCTACTCAT CCTTACTTTT CAGCACAGCT TGGGGCAGGG
    CAGCTGTCGC TCTTTAATCT CTTGAAAGCC TACTCATTAC TGGACAAAGA AGTGGGTTAC
    TGTCAGGGGA TCAGCTTTGT GGCTGGAGTC TTGCTTCTAC ACATGAGTGA AGAGCAAGCC
    TTTGAAATGC TGAAATTCCT CATGTATGAC CTGGGCTTCC GCAAGCAGTA CCGACCTGAC
    ATGATGTCGC TGCAGATTCA AATGTACCAG CTGTCCAGGC TCCTTCATGA TTATCACAGA
    GATCTCTACA ATCATCTTGA AGAAAATGAA ATCAGCCCCA GTCTTTATGC TGCCCCCTGG
    TTCCTCACGT TGTTTGCCTC TCAATTTCCA TTAGGATTTG TAGCCAGAGT GTTTGATATT
    ATTTTTCTTC AGGGAACTGA AGTTATATTT AAGGTTGCCC TAAGCCTGTT GAGCAACCAA
    GAGACACTCA TAATGGAATG TGAAAACTTT GAAAATATTG TTGAATTCCT TAAAATCACT
    CTCCCCGATA TGAATTCATC TGAAATGGAA AAAATAATCA CCCAGGTTTT TGAGATGGAT
    ATTTCTAAAC AGTTACATGC CTATGAGGTG GAGTATCACG TGCTACAGGA TGAACTCCAA
    GAATCTTCCT ATGCTTGCGA GGACAGTGAG CCCATAGAGA AGCTGGAGAG GGCCAACAGC
    CAACTGAAAA GACAAAACAT GGACCTTCTA GAAAAATTAC AGATAGCTCA TGCTAAAATT
    CAGACCTTGG AGTCCAACCT GGAAAATCTT TTAACCAGAG AGACCAAAAT GAAGTCTTTG
    ATCCGGACCC TGGAACAGGA GAAGACGGCT TATCAGAAGG CAGTGGAGCA GATCTGGAAG
    CTGCTGCCTG CAGATATTTT AGCCAACTGC GAACCACTGC TGAGAGACTT AAACTGCAAC
    CCTAACAACA AAGCCAAGAC AGGAAACAAG CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213901.1
    CDS369..4244
    Translation

    Target ORF information:

    RefSeq Version XM_005213844.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213844.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAATGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGACT
    ATTTCAAATA GTACAATCCC GGAAAATGCG ACAAGCAGTG GAAGGTTCAA ACTTGACATT
    CTGAAAAATA AAGCTAAGAG ATCCTTAACT AGCTCCCTGG AAAATATCTT CTCAAGGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC GTTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGC
    ATCCTTCCAC CAGCTCGGCG CATGCACAAG GAGTGTAATT CTTCCTCCAG TCTTCCAAGT
    CTTCACACTT CCTTCTCTGC CCCTTCCTTC ACTGCCCCCC CTTTCTTGAA AAGCTTTTAC
    CAGAATTCAG GTAGACTATC CCCACAGTAT GAGAATGAAA TCAGTGATGG AGAGGGGAGA
    AAAAGGACCT CGTCCACCTG CAGCAATGAA TCCCTAAACG CTGGAGGAAC CCCCGTCACT
    CCTCGCCGAG TTTCCTGGCG GCAGCGCATC TTCCTCAGGG TTGCTTCGCC CATGAGGAAA
    TCTCCCTCAG CGATGCAGCA GGATGGCCTG GACAGGAGTG AGCTGCTGCC ACTGTCCCCT
    CTTGCTCCAA CCATGGAGGA GGAACCTCTA ATGATATTCA TGTCTGATGA AGATGACCCA
    GAAAAGGGTG AAGAGAGAAA CAAGTCGGAG GAACTGAGGT GTTTGTGGAG AAAAGCTATA
    CACCAGCAGA TCCTGTTGCT TCGAATGGAA AAAGAAAACC AGAAACTTGA AGCAAGCAGA
    GATGAACTGC AGTCCAGAAA AGTTAAATTG GATTATGAAG AAGTTGGTGC ATGTCAGAAG
    GAGGTCTTAA CAATCTGGGA TAAGAAGTTG TTAAACTGCA GAGCTAAAAT CAGATGCGAT
    ATGGAAGATA TTCATACTTC TCTTAAAGAA GGTGTTCCCA AAAGTCGACG AGGAGAAATC
    TGGCAGTTCC TAGCTTTGCA ATATCGTCTG AGACACAGAT TGCCTAATAA ACAACAACCT
    CCCGACATAT CCTATAAAGA GCTTTTAAAA CAGCTCACGG CTCAGCAGCA TGCTATCCTC
    GTGGATTTAG GGAGAACATT TCCTACTCAT CCTTACTTTT CAGCACAGCT TGGGGCAGGG
    CAGCTGTCGC TCTTTAATCT CTTGAAAGCC TACTCATTAC TGGACAAAGA AGTGGGTTAC
    TGTCAGGGGA TCAGCTTTGT GGCTGGAGTC TTGCTTCTAC ACATGAGTGA AGAGCAAGCC
    TTTGAAATGC TGAAATTCCT CATGTATGAC CTGGGCTTCC GCAAGCAGTA CCGACCTGAC
    ATGATGTCGC TGCAGATTCA AATGTACCAG CTGTCCAGGC TCCTTCATGA TTATCACAGA
    GATCTCTACA ATCATCTTGA AGAAAATGAA ATCAGCCCCA GTCTTTATGC TGCCCCCTGG
    TTCCTCACGT TGTTTGCCTC TCAATTTCCA TTAGGATTTG TAGCCAGAGT GTTTGATATT
    ATTTTTCTTC AGGGAACTGA AGTTATATTT AAGGTTGCCC TAAGCCTGTT GAGCAACCAA
    GAGACACTCA TAATGGAATG TGAAAACTTT GAAAATATTG TTGAATTCCT TAAAATCACT
    CTCCCCGATA TGAATTCATC TGAAATGGAA AAAATAATCA CCCAGGTTTT TGAGATGGAT
    ATTTCTAAAC AGTTACATGC CTATGAGGTG GAGTATCACG TGCTACAGGA TGAACTCCAA
    GAATCTTCCT ATGCTTGCGA GGACAGTGAG CCCATAGAGA AGCTGGAGAG GGCCAACAGC
    CAACTGAAAA GACAAAACAT GGACCTTCTA GAAAAATTAC AGATAGCTCA TGCTAAAATT
    CAGACCTTGG AGTCCAACCT GGAAAATCTT TTAACCAGAG AGACCAAAAT GAAGTCTTTG
    ATCCGGACCC TGGAACAGGA GAAGACGGCT TATCAGAAGG CAGTGGAGCA GATCTGGAAG
    CTGCTGCCTG CAGATATTTT AGCCAACTGC GAACCACTGC TGAGAGACTT AAACTGCAAC
    CCTAACAACA AAGCCAAGAC AGGAAACAAG CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221159
    Clone ID Related Accession (Same CDS sequence) XM_024999914.1
    Accession Version XM_024999914.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAATGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC GTTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGC
    ATCCTTCCAC CAGCTCGGCG CATGCACAAG GAGTGTAATT CTTCCTCCAG TCTTCCAAGT
    CTTCACACTT CCTTCTCTGC CCCTTCCTTC ACTGCCCCCC CTTTCTTGAA AAGCTTTTAC
    CAGAATTCAG GTAGACTATC CCCACAGTAT GAGAATGAAA TCAGTGATGG AGAGGGGAGA
    AAAAGGACCT CGTCCACCTG CAGCAATGAA TCCCTAAACG CTGGAGGAAC CCCCGTCACT
    CCTCGCCGAG TTTCCTGGCG GCAGCGCATC TTCCTCAGGG TTGCTTCGCC CATGAGGAAA
    TCTCCCTCAG CGATGCAGCA GGATGGCCTG GACAGGAGTG AGCTGCTGCC ACTGTCCCCT
    CTTGCTCCAA CCATGGAGGA GGAACCTCTA ATGATATTCA TGTCTGATGA AGATGACCCA
    GAAAAGGGTG AAGAGAGAAA CAAGTCGGAG GAACTGAGGT GTTTGTGGAG AAAAGCTATA
    CACCAGCAGA TCCTGTTGCT TCGAATGGAA AAAGAAAACC AGAAACTTGA AGCAAGCAGA
    GATGAACTGC AGTCCAGAAA AGTTAAATTG GATTATGAAG AAGTTGGTGC ATGTCAGAAG
    GAGGTCTTAA CAATCTGGGA TAAGAAGTTG TTAAACTGCA GAGCTAAAAT CAGATGCGAT
    ATGGAAGATA TTCATACTTC TCTTAAAGAA GGTGTTCCCA AAAGTCGACG AGGAGAAATC
    TGGCAGTTCC TAGCTTTGCA ATATCGTCTG AGACACAGAT TGCCTAATAA ACAACAACCT
    CCCGACATAT CCTATAAAGA GCTTTTAAAA CAGCTCACGG CTCAGCAGCA TGCTATCCTC
    GTGGATTTAG GGAGAACATT TCCTACTCAT CCTTACTTTT CAGCACAGCT TGGGGCAGGG
    CAGCTGTCGC TCTTTAATCT CTTGAAAGCC TACTCATTAC TGGACAAAGA AGTGGGTTAC
    TGTCAGGGGA TCAGCTTTGT GGCTGGAGTC TTGCTTCTAC ACATGAGTGA AGAGCAAGCC
    TTTGAAATGC TGAAATTCCT CATGTATGAC CTGGGCTTCC GCAAGCAGTA CCGACCTGAC
    ATGATGTCGC TGCAGATTCA AATGTACCAG CTGTCCAGGC TCCTTCATGA TTATCACAGA
    GATCTCTACA ATCATCTTGA AGAAAATGAA ATCAGCCCCA GTCTTTATGC TGCCCCCTGG
    TTCCTCACGT TGTTTGCCTC TCAATTTCCA TTAGGATTTG TAGCCAGAGT GTTTGATATT
    ATTTTTCTTC AGGGAACTGA AGTTATATTT AAGGTTGCCC TAAGCCTGTT GAGCAACCAA
    GAGACACTCA TAATGGAATG TGAAAACTTT GAAAATATTG TTGAATTCCT TAAAATCACT
    CTCCCCGATA TGAATTCATC TGAAATGGAA AAAATAATCA CCCAGGTTTT TGAGATGGAT
    ATTTCTAAAC AGTTACATGC CTATGAGGTG GAGTATCACG TGCTACAGGA TGAACTCCAA
    GAATCTTCCT ATGCTTGCGA GGACAGTGAG CCCATAGAGA AGCTGGAGAG GGCCAACAGC
    CAACTGAAAA GACAAAACAT GGACCTTCTA GAAAAATTAC AGATAGCTCA TGCTAAAATT
    CAGACCTTGG AGTCCAACCT GGAAAATCTT TTAACCAGAG AGACCAAAAT GAAGTCTTTG
    ATCCGGACCC TGGAACAGGA GAAGACGGCT TATCAGAAGG CAGTGGAGCA GATCTGGAAG
    CTGCTGCCTG CAGATATTTT AGCCAACTGC GAACCACTGC TGAGAGACTT AAACTGCAAC
    CCTAACAACA AAGCCAAGAC AGGAAACAAG CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855682.1
    CDS369..4124
    Translation

    Target ORF information:

    RefSeq Version XM_024999914.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999914.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAATGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC GTTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGC
    ATCCTTCCAC CAGCTCGGCG CATGCACAAG GAGTGTAATT CTTCCTCCAG TCTTCCAAGT
    CTTCACACTT CCTTCTCTGC CCCTTCCTTC ACTGCCCCCC CTTTCTTGAA AAGCTTTTAC
    CAGAATTCAG GTAGACTATC CCCACAGTAT GAGAATGAAA TCAGTGATGG AGAGGGGAGA
    AAAAGGACCT CGTCCACCTG CAGCAATGAA TCCCTAAACG CTGGAGGAAC CCCCGTCACT
    CCTCGCCGAG TTTCCTGGCG GCAGCGCATC TTCCTCAGGG TTGCTTCGCC CATGAGGAAA
    TCTCCCTCAG CGATGCAGCA GGATGGCCTG GACAGGAGTG AGCTGCTGCC ACTGTCCCCT
    CTTGCTCCAA CCATGGAGGA GGAACCTCTA ATGATATTCA TGTCTGATGA AGATGACCCA
    GAAAAGGGTG AAGAGAGAAA CAAGTCGGAG GAACTGAGGT GTTTGTGGAG AAAAGCTATA
    CACCAGCAGA TCCTGTTGCT TCGAATGGAA AAAGAAAACC AGAAACTTGA AGCAAGCAGA
    GATGAACTGC AGTCCAGAAA AGTTAAATTG GATTATGAAG AAGTTGGTGC ATGTCAGAAG
    GAGGTCTTAA CAATCTGGGA TAAGAAGTTG TTAAACTGCA GAGCTAAAAT CAGATGCGAT
    ATGGAAGATA TTCATACTTC TCTTAAAGAA GGTGTTCCCA AAAGTCGACG AGGAGAAATC
    TGGCAGTTCC TAGCTTTGCA ATATCGTCTG AGACACAGAT TGCCTAATAA ACAACAACCT
    CCCGACATAT CCTATAAAGA GCTTTTAAAA CAGCTCACGG CTCAGCAGCA TGCTATCCTC
    GTGGATTTAG GGAGAACATT TCCTACTCAT CCTTACTTTT CAGCACAGCT TGGGGCAGGG
    CAGCTGTCGC TCTTTAATCT CTTGAAAGCC TACTCATTAC TGGACAAAGA AGTGGGTTAC
    TGTCAGGGGA TCAGCTTTGT GGCTGGAGTC TTGCTTCTAC ACATGAGTGA AGAGCAAGCC
    TTTGAAATGC TGAAATTCCT CATGTATGAC CTGGGCTTCC GCAAGCAGTA CCGACCTGAC
    ATGATGTCGC TGCAGATTCA AATGTACCAG CTGTCCAGGC TCCTTCATGA TTATCACAGA
    GATCTCTACA ATCATCTTGA AGAAAATGAA ATCAGCCCCA GTCTTTATGC TGCCCCCTGG
    TTCCTCACGT TGTTTGCCTC TCAATTTCCA TTAGGATTTG TAGCCAGAGT GTTTGATATT
    ATTTTTCTTC AGGGAACTGA AGTTATATTT AAGGTTGCCC TAAGCCTGTT GAGCAACCAA
    GAGACACTCA TAATGGAATG TGAAAACTTT GAAAATATTG TTGAATTCCT TAAAATCACT
    CTCCCCGATA TGAATTCATC TGAAATGGAA AAAATAATCA CCCAGGTTTT TGAGATGGAT
    ATTTCTAAAC AGTTACATGC CTATGAGGTG GAGTATCACG TGCTACAGGA TGAACTCCAA
    GAATCTTCCT ATGCTTGCGA GGACAGTGAG CCCATAGAGA AGCTGGAGAG GGCCAACAGC
    CAACTGAAAA GACAAAACAT GGACCTTCTA GAAAAATTAC AGATAGCTCA TGCTAAAATT
    CAGACCTTGG AGTCCAACCT GGAAAATCTT TTAACCAGAG AGACCAAAAT GAAGTCTTTG
    ATCCGGACCC TGGAACAGGA GAAGACGGCT TATCAGAAGG CAGTGGAGCA GATCTGGAAG
    CTGCTGCCTG CAGATATTTT AGCCAACTGC GAACCACTGC TGAGAGACTT AAACTGCAAC
    CCTAACAACA AAGCCAAGAC AGGAAACAAG CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221160
    Clone ID Related Accession (Same CDS sequence) XM_005213847.4
    Accession Version XM_005213847.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213847.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAATGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGACT
    ATTTCAAATA GTACAATCCC GGAAAATGCG ACAAGCAGTG GAAGGTTCAA ACTTGACATT
    CTGAAAAATA AAGCTAAGAG ATCCTTAACT AGCTCCCTGG AAAATATCTT CTCAAGGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC GTTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGT
    GATGGAGAGG GGAGAAAAAG GACCTCGTCC ACCTGCAGCA ATGAATCCCT AAACGCTGGA
    GGAACCCCCG TCACTCCTCG CCGAGTTTCC TGGCGGCAGC GCATCTTCCT CAGGGTTGCT
    TCGCCCATGA GGAAATCTCC CTCAGCGATG CAGCAGGATG GCCTGGACAG GAGTGAGCTG
    CTGCCACTGT CCCCTCTTGC TCCAACCATG GAGGAGGAAC CTCTAATGAT ATTCATGTCT
    GATGAAGATG ACCCAGAAAA GGGTGAAGAG AGAAACAAGT CGGAGGAACT GAGGTGTTTG
    TGGAGAAAAG CTATACACCA GCAGATCCTG TTGCTTCGAA TGGAAAAAGA AAACCAGAAA
    CTTGAAGCAA GCAGAGATGA ACTGCAGTCC AGAAAAGTTA AATTGGATTA TGAAGAAGTT
    GGTGCATGTC AGAAGGAGGT CTTAACAATC TGGGATAAGA AGTTGTTAAA CTGCAGAGCT
    AAAATCAGAT GCGATATGGA AGATATTCAT ACTTCTCTTA AAGAAGGTGT TCCCAAAAGT
    CGACGAGGAG AAATCTGGCA GTTCCTAGCT TTGCAATATC GTCTGAGACA CAGATTGCCT
    AATAAACAAC AACCTCCCGA CATATCCTAT AAAGAGCTTT TAAAACAGCT CACGGCTCAG
    CAGCATGCTA TCCTCGTGGA TTTAGGGAGA ACATTTCCTA CTCATCCTTA CTTTTCAGCA
    CAGCTTGGGG CAGGGCAGCT GTCGCTCTTT AATCTCTTGA AAGCCTACTC ATTACTGGAC
    AAAGAAGTGG GTTACTGTCA GGGGATCAGC TTTGTGGCTG GAGTCTTGCT TCTACACATG
    AGTGAAGAGC AAGCCTTTGA AATGCTGAAA TTCCTCATGT ATGACCTGGG CTTCCGCAAG
    CAGTACCGAC CTGACATGAT GTCGCTGCAG ATTCAAATGT ACCAGCTGTC CAGGCTCCTT
    CATGATTATC ACAGAGATCT CTACAATCAT CTTGAAGAAA ATGAAATCAG CCCCAGTCTT
    TATGCTGCCC CCTGGTTCCT CACGTTGTTT GCCTCTCAAT TTCCATTAGG ATTTGTAGCC
    AGAGTGTTTG ATATTATTTT TCTTCAGGGA ACTGAAGTTA TATTTAAGGT TGCCCTAAGC
    CTGTTGAGCA ACCAAGAGAC ACTCATAATG GAATGTGAAA ACTTTGAAAA TATTGTTGAA
    TTCCTTAAAA TCACTCTCCC CGATATGAAT TCATCTGAAA TGGAAAAAAT AATCACCCAG
    GTTTTTGAGA TGGATATTTC TAAACAGTTA CATGCCTATG AGGTGGAGTA TCACGTGCTA
    CAGGATGAAC TCCAAGAATC TTCCTATGCT TGCGAGGACA GTGAGCCCAT AGAGAAGCTG
    GAGAGGGCCA ACAGCCAACT GAAAAGACAA AACATGGACC TTCTAGAAAA ATTACAGATA
    GCTCATGCTA AAATTCAGAC CTTGGAGTCC AACCTGGAAA ATCTTTTAAC CAGAGAGACC
    AAAATGAAGT CTTTGATCCG GACCCTGGAA CAGGAGAAGA CGGCTTATCA GAAGGCAGTG
    GAGCAGATCT GGAAGCTGCT GCCTGCAGAT ATTTTAGCCA ACTGCGAACC ACTGCTGAGA
    GACTTAAACT GCAACCCTAA CAACAAAGCC AAGACAGGAA ACAAGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213904.1
    CDS570..4280
    Translation

    Target ORF information:

    RefSeq Version XM_005213847.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213847.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAATGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGACT
    ATTTCAAATA GTACAATCCC GGAAAATGCG ACAAGCAGTG GAAGGTTCAA ACTTGACATT
    CTGAAAAATA AAGCTAAGAG ATCCTTAACT AGCTCCCTGG AAAATATCTT CTCAAGGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC GTTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGT
    GATGGAGAGG GGAGAAAAAG GACCTCGTCC ACCTGCAGCA ATGAATCCCT AAACGCTGGA
    GGAACCCCCG TCACTCCTCG CCGAGTTTCC TGGCGGCAGC GCATCTTCCT CAGGGTTGCT
    TCGCCCATGA GGAAATCTCC CTCAGCGATG CAGCAGGATG GCCTGGACAG GAGTGAGCTG
    CTGCCACTGT CCCCTCTTGC TCCAACCATG GAGGAGGAAC CTCTAATGAT ATTCATGTCT
    GATGAAGATG ACCCAGAAAA GGGTGAAGAG AGAAACAAGT CGGAGGAACT GAGGTGTTTG
    TGGAGAAAAG CTATACACCA GCAGATCCTG TTGCTTCGAA TGGAAAAAGA AAACCAGAAA
    CTTGAAGCAA GCAGAGATGA ACTGCAGTCC AGAAAAGTTA AATTGGATTA TGAAGAAGTT
    GGTGCATGTC AGAAGGAGGT CTTAACAATC TGGGATAAGA AGTTGTTAAA CTGCAGAGCT
    AAAATCAGAT GCGATATGGA AGATATTCAT ACTTCTCTTA AAGAAGGTGT TCCCAAAAGT
    CGACGAGGAG AAATCTGGCA GTTCCTAGCT TTGCAATATC GTCTGAGACA CAGATTGCCT
    AATAAACAAC AACCTCCCGA CATATCCTAT AAAGAGCTTT TAAAACAGCT CACGGCTCAG
    CAGCATGCTA TCCTCGTGGA TTTAGGGAGA ACATTTCCTA CTCATCCTTA CTTTTCAGCA
    CAGCTTGGGG CAGGGCAGCT GTCGCTCTTT AATCTCTTGA AAGCCTACTC ATTACTGGAC
    AAAGAAGTGG GTTACTGTCA GGGGATCAGC TTTGTGGCTG GAGTCTTGCT TCTACACATG
    AGTGAAGAGC AAGCCTTTGA AATGCTGAAA TTCCTCATGT ATGACCTGGG CTTCCGCAAG
    CAGTACCGAC CTGACATGAT GTCGCTGCAG ATTCAAATGT ACCAGCTGTC CAGGCTCCTT
    CATGATTATC ACAGAGATCT CTACAATCAT CTTGAAGAAA ATGAAATCAG CCCCAGTCTT
    TATGCTGCCC CCTGGTTCCT CACGTTGTTT GCCTCTCAAT TTCCATTAGG ATTTGTAGCC
    AGAGTGTTTG ATATTATTTT TCTTCAGGGA ACTGAAGTTA TATTTAAGGT TGCCCTAAGC
    CTGTTGAGCA ACCAAGAGAC ACTCATAATG GAATGTGAAA ACTTTGAAAA TATTGTTGAA
    TTCCTTAAAA TCACTCTCCC CGATATGAAT TCATCTGAAA TGGAAAAAAT AATCACCCAG
    GTTTTTGAGA TGGATATTTC TAAACAGTTA CATGCCTATG AGGTGGAGTA TCACGTGCTA
    CAGGATGAAC TCCAAGAATC TTCCTATGCT TGCGAGGACA GTGAGCCCAT AGAGAAGCTG
    GAGAGGGCCA ACAGCCAACT GAAAAGACAA AACATGGACC TTCTAGAAAA ATTACAGATA
    GCTCATGCTA AAATTCAGAC CTTGGAGTCC AACCTGGAAA ATCTTTTAAC CAGAGAGACC
    AAAATGAAGT CTTTGATCCG GACCCTGGAA CAGGAGAAGA CGGCTTATCA GAAGGCAGTG
    GAGCAGATCT GGAAGCTGCT GCCTGCAGAT ATTTTAGCCA ACTGCGAACC ACTGCTGAGA
    GACTTAAACT GCAACCCTAA CAACAAAGCC AAGACAGGAA ACAAGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221161
    Clone ID Related Accession (Same CDS sequence) XM_024999915.1
    Accession Version XM_024999915.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAAGAGG ATGCAAAACC CAGCAAAGAC AACGAGGACG CCTTTTACAA CTCTCAGAAG 
    TTCGAGGTCC TGTACTGTGG CAGGGTGACC GTGACCCACA AGAAGGCGCC GTCCAGCCTC
    ATCGACGACT GCATCGAGAA GTTCAGCCTG CACGAGCAGC AGCGCTTGCG GCTCCAGAGC
    GAGCCGCGCG CAGGGGACTC GGGCGAAGAC CAGGCGGGAT CCGAGGCCCG AACGCCGGCC
    TGCCCCGTCG ACGTTCTGTC GGAGGAAGGG GATGGCGTCG CCGACACCCA CCCGGTCTTC
    CCTGCCGCGC CCAGCCAGCC TGGGCCATCT AGCTCGCGAG TCTGCTTCCC TGAGAGGATT
    TTGGAAGATT CGGGCTTCGA TGAACAGCAG GAGTTCCGGT CTCGGTGCAG CAGCGTCACG
    GGGGTCCTGC AAAGGAAAGT TCACGAGAAC AGCCAGAAGC CGCAGCCGCG GCGGAGGCAT
    GCCAGCGCGC CCAGCCACGT GCAGCCCTCG GACTCGGAGA AGAACAGAAC CATGCTGTTT
    CAGGTTGGGC GGTTTGAGAT TAACCTTATC AGTCCGGACA CTAAATCAGT TGTGCTTGAG
    AAGAATTTTA AAGATATCTC GTCTTGTTCC CAGGGTATAA AGCATGTTGA TCACTTCGGC
    TTTATTTGTC GGGAGTCCTC GGAGCCTGGA CTGAGCCAGT ATATCTGCTA CGTGTTCCAG
    TGTGCCAGTG AATCTCTGGT TGATGAGGTA ATGCTGACTC TGAAACAGGC TTTCAGCACG
    GCCGCTGCCC TGCAGAGTGC CAAGACCCAG ATTAAGCTGT GTGAGGCCTG CCCCATGCAC
    TCTTTGCATA AGCTCTGTGA AAGGATCGAA GGTCTCTACC CACCAAGAGC CAAGCTGGTA
    ATACAGAGGC ATCTCTCATC CCTGACAGAT AATGAGCAAG CTGACATCTT TGAACGCGTT
    CAGAAAATGA AGGCAGTCAA TGACCAGGAG GAGAATGAAC TTGTGATTTT ACATCTGAGG
    CAGCTATGCG AAGCCAAGCA GAAGACACAT GTTCATATCG GGGAAGGCCC ATTGACTATT
    TCAAATAGTA CAATCCCGGA AAATGCGACA AGCAGTGGAA GGTTCAAACT TGACATTCTG
    AAAAATAAAG CTAAGAGATC CTTAACTAGC TCCCTGGAAA ATATCTTCTC AAGGGGAGCT
    AACAGAATGA GAGGTCGGCT TGGAAGTGTG GACAGCTTTG AACGATCCAA CAGTGTTGCT
    TCAGAGAAGG ACTACTCGCC AGGAGACTCT CCGCCAGGGA CCCCGCCAGC TTCCCCCCTG
    TCCTCAGCTT GGCACACGTT CCCTGAGGAG GATTCCGACT CCCCGCAGTT TCGAAGACGG
    GCCCACACAT TCAGCCACCC ACCTTCAAGC ACGAAGAGGA AGCTGAATTT GCAGGATGGG
    AAGGCCCATG GGGTTCGCTC CCCTCTTCTG AGGCAGAGCT CCAGTGAGCA GTGCAGCATC
    CTTCCACCAG CTCGGCGCAT GCACAAGGAG TGTAATTCTT CCTCCAGTCT TCCAAGTCTT
    CACACTTCCT TCTCTGCCCC TTCCTTCACT GCCCCCCCTT TCTTGAAAAG CTTTTACCAG
    AATTCAGGTA GACTATCCCC ACAGTATGAG AATGAAATCA GTGATGGAGA GGGGAGAAAA
    AGGACCTCGT CCACCTGCAG CAATGAATCC CTAAACGCTG GAGGAACCCC CGTCACTCCT
    CGCCGAGTTT CCTGGCGGCA GCGCATCTTC CTCAGGGTTG CTTCGCCCAT GAGGAAATCT
    CCCTCAGCGA TGCAGCAGGA TGGCCTGGAC AGGAGTGAGC TGCTGCCACT GTCCCCTCTT
    GCTCCAACCA TGGAGGAGGA ACCTCTAATG ATATTCATGT CTGATGAAGA TGACCCAGAA
    AAGGGTGAAG AGAGAAACAA GTCGGAGGAA CTGAGGTGTT TGTGGAGAAA AGCTATACAC
    CAGCAGATCC TGTTGCTTCG AATGGAAAAA GAAAACCAGA AACTTGAAGC AAGCAGAGAT
    GAACTGCAGT CCAGAAAAGT TAAATTGGAT TATGAAGAAG TTGGTGCATG TCAGAAGGAG
    GTCTTAACAA TCTGGGATAA GAAGTTGTTA AACTGCAGAG CTAAAATCAG ATGCGATATG
    GAAGATATTC ATACTTCTCT TAAAGAAGGT GTTCCCAAAA GTCGACGAGG AGAAATCTGG
    CAGTTCCTAG CTTTGCAATA TCGTCTGAGA CACAGATTGC CTAATAAACA ACAACCTCCC
    GACATATCCT ATAAAGAGCT TTTAAAACAG CTCACGGCTC AGCAGCATGC TATCCTCGTG
    GATTTAGGGA GAACATTTCC TACTCATCCT TACTTTTCAG CACAGCTTGG GGCAGGGCAG
    CTGTCGCTCT TTAATCTCTT GAAAGCCTAC TCATTACTGG ACAAAGAAGT GGGTTACTGT
    CAGGGGATCA GCTTTGTGGC TGGAGTCTTG CTTCTACACA TGAGTGAAGA GCAAGCCTTT
    GAAATGCTGA AATTCCTCAT GTATGACCTG GGCTTCCGCA AGCAGTACCG ACCTGACATG
    ATGTCGCTGC AGATTCAAAT GTACCAGCTG TCCAGGCTCC TTCATGATTA TCACAGAGAT
    CTCTACAATC ATCTTGAAGA AAATGAAATC AGCCCCAGTC TTTATGCTGC CCCCTGGTTC
    CTCACGTTGT TTGCCTCTCA ATTTCCATTA GGATTTGTAG CCAGAGTGTT TGATATTATT
    TTTCTTCAGG GAACTGAAGT TATATTTAAG GTTGCCCTAA GCCTGTTGAG CAACCAAGAG
    ACACTCATAA TGGAATGTGA AAACTTTGAA AATATTGTTG AATTCCTTAA AATCACTCTC
    CCCGATATGA ATTCATCTGA AATGGAAAAA ATAATCACCC AGGTTTTTGA GATGGATATT
    TCTAAACAGT TACATGCCTA TGAGGTGGAG TATCACGTGC TACAGGATGA ACTCCAAGAA
    TCTTCCTATG CTTGCGAGGA CAGTGAGCCC ATAGAGAAGC TGGAGAGGGC CAACAGCCAA
    CTGAAAAGAC AAAACATGGA CCTTCTAGAA AAATTACAGA TAGCTCATGC TAAAATTCAG
    ACCTTGGAGT CCAACCTGGA AAATCTTTTA ACCAGAGAGA CCAAAATGAA GTCTTTGATC
    CGGACCCTGG AACAGGAGAA GACGGCTTAT CAGAAGGCAG TGGAGCAGAT CTGGAAGCTG
    CTGCCTGCAG ATATTTTAGC CAACTGCGAA CCACTGCTGA GAGACTTAAA CTGCAACCCT
    AACAACAAAG CCAAGACAGG AAACAAGCCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855683.1
    CDS147..3479
    Translation

    Target ORF information:

    RefSeq Version XM_024999915.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAAGAGG ATGCAAAACC CAGCAAAGAC AACGAGGACG CCTTTTACAA CTCTCAGAAG 
    TTCGAGGTCC TGTACTGTGG CAGGGTGACC GTGACCCACA AGAAGGCGCC GTCCAGCCTC
    ATCGACGACT GCATCGAGAA GTTCAGCCTG CACGAGCAGC AGCGCTTGCG GCTCCAGAGC
    GAGCCGCGCG CAGGGGACTC GGGCGAAGAC CAGGCGGGAT CCGAGGCCCG AACGCCGGCC
    TGCCCCGTCG ACGTTCTGTC GGAGGAAGGG GATGGCGTCG CCGACACCCA CCCGGTCTTC
    CCTGCCGCGC CCAGCCAGCC TGGGCCATCT AGCTCGCGAG TCTGCTTCCC TGAGAGGATT
    TTGGAAGATT CGGGCTTCGA TGAACAGCAG GAGTTCCGGT CTCGGTGCAG CAGCGTCACG
    GGGGTCCTGC AAAGGAAAGT TCACGAGAAC AGCCAGAAGC CGCAGCCGCG GCGGAGGCAT
    GCCAGCGCGC CCAGCCACGT GCAGCCCTCG GACTCGGAGA AGAACAGAAC CATGCTGTTT
    CAGGTTGGGC GGTTTGAGAT TAACCTTATC AGTCCGGACA CTAAATCAGT TGTGCTTGAG
    AAGAATTTTA AAGATATCTC GTCTTGTTCC CAGGGTATAA AGCATGTTGA TCACTTCGGC
    TTTATTTGTC GGGAGTCCTC GGAGCCTGGA CTGAGCCAGT ATATCTGCTA CGTGTTCCAG
    TGTGCCAGTG AATCTCTGGT TGATGAGGTA ATGCTGACTC TGAAACAGGC TTTCAGCACG
    GCCGCTGCCC TGCAGAGTGC CAAGACCCAG ATTAAGCTGT GTGAGGCCTG CCCCATGCAC
    TCTTTGCATA AGCTCTGTGA AAGGATCGAA GGTCTCTACC CACCAAGAGC CAAGCTGGTA
    ATACAGAGGC ATCTCTCATC CCTGACAGAT AATGAGCAAG CTGACATCTT TGAACGCGTT
    CAGAAAATGA AGGCAGTCAA TGACCAGGAG GAGAATGAAC TTGTGATTTT ACATCTGAGG
    CAGCTATGCG AAGCCAAGCA GAAGACACAT GTTCATATCG GGGAAGGCCC ATTGACTATT
    TCAAATAGTA CAATCCCGGA AAATGCGACA AGCAGTGGAA GGTTCAAACT TGACATTCTG
    AAAAATAAAG CTAAGAGATC CTTAACTAGC TCCCTGGAAA ATATCTTCTC AAGGGGAGCT
    AACAGAATGA GAGGTCGGCT TGGAAGTGTG GACAGCTTTG AACGATCCAA CAGTGTTGCT
    TCAGAGAAGG ACTACTCGCC AGGAGACTCT CCGCCAGGGA CCCCGCCAGC TTCCCCCCTG
    TCCTCAGCTT GGCACACGTT CCCTGAGGAG GATTCCGACT CCCCGCAGTT TCGAAGACGG
    GCCCACACAT TCAGCCACCC ACCTTCAAGC ACGAAGAGGA AGCTGAATTT GCAGGATGGG
    AAGGCCCATG GGGTTCGCTC CCCTCTTCTG AGGCAGAGCT CCAGTGAGCA GTGCAGCATC
    CTTCCACCAG CTCGGCGCAT GCACAAGGAG TGTAATTCTT CCTCCAGTCT TCCAAGTCTT
    CACACTTCCT TCTCTGCCCC TTCCTTCACT GCCCCCCCTT TCTTGAAAAG CTTTTACCAG
    AATTCAGGTA GACTATCCCC ACAGTATGAG AATGAAATCA GTGATGGAGA GGGGAGAAAA
    AGGACCTCGT CCACCTGCAG CAATGAATCC CTAAACGCTG GAGGAACCCC CGTCACTCCT
    CGCCGAGTTT CCTGGCGGCA GCGCATCTTC CTCAGGGTTG CTTCGCCCAT GAGGAAATCT
    CCCTCAGCGA TGCAGCAGGA TGGCCTGGAC AGGAGTGAGC TGCTGCCACT GTCCCCTCTT
    GCTCCAACCA TGGAGGAGGA ACCTCTAATG ATATTCATGT CTGATGAAGA TGACCCAGAA
    AAGGGTGAAG AGAGAAACAA GTCGGAGGAA CTGAGGTGTT TGTGGAGAAA AGCTATACAC
    CAGCAGATCC TGTTGCTTCG AATGGAAAAA GAAAACCAGA AACTTGAAGC AAGCAGAGAT
    GAACTGCAGT CCAGAAAAGT TAAATTGGAT TATGAAGAAG TTGGTGCATG TCAGAAGGAG
    GTCTTAACAA TCTGGGATAA GAAGTTGTTA AACTGCAGAG CTAAAATCAG ATGCGATATG
    GAAGATATTC ATACTTCTCT TAAAGAAGGT GTTCCCAAAA GTCGACGAGG AGAAATCTGG
    CAGTTCCTAG CTTTGCAATA TCGTCTGAGA CACAGATTGC CTAATAAACA ACAACCTCCC
    GACATATCCT ATAAAGAGCT TTTAAAACAG CTCACGGCTC AGCAGCATGC TATCCTCGTG
    GATTTAGGGA GAACATTTCC TACTCATCCT TACTTTTCAG CACAGCTTGG GGCAGGGCAG
    CTGTCGCTCT TTAATCTCTT GAAAGCCTAC TCATTACTGG ACAAAGAAGT GGGTTACTGT
    CAGGGGATCA GCTTTGTGGC TGGAGTCTTG CTTCTACACA TGAGTGAAGA GCAAGCCTTT
    GAAATGCTGA AATTCCTCAT GTATGACCTG GGCTTCCGCA AGCAGTACCG ACCTGACATG
    ATGTCGCTGC AGATTCAAAT GTACCAGCTG TCCAGGCTCC TTCATGATTA TCACAGAGAT
    CTCTACAATC ATCTTGAAGA AAATGAAATC AGCCCCAGTC TTTATGCTGC CCCCTGGTTC
    CTCACGTTGT TTGCCTCTCA ATTTCCATTA GGATTTGTAG CCAGAGTGTT TGATATTATT
    TTTCTTCAGG GAACTGAAGT TATATTTAAG GTTGCCCTAA GCCTGTTGAG CAACCAAGAG
    ACACTCATAA TGGAATGTGA AAACTTTGAA AATATTGTTG AATTCCTTAA AATCACTCTC
    CCCGATATGA ATTCATCTGA AATGGAAAAA ATAATCACCC AGGTTTTTGA GATGGATATT
    TCTAAACAGT TACATGCCTA TGAGGTGGAG TATCACGTGC TACAGGATGA ACTCCAAGAA
    TCTTCCTATG CTTGCGAGGA CAGTGAGCCC ATAGAGAAGC TGGAGAGGGC CAACAGCCAA
    CTGAAAAGAC AAAACATGGA CCTTCTAGAA AAATTACAGA TAGCTCATGC TAAAATTCAG
    ACCTTGGAGT CCAACCTGGA AAATCTTTTA ACCAGAGAGA CCAAAATGAA GTCTTTGATC
    CGGACCCTGG AACAGGAGAA GACGGCTTAT CAGAAGGCAG TGGAGCAGAT CTGGAAGCTG
    CTGCCTGCAG ATATTTTAGC CAACTGCGAA CCACTGCTGA GAGACTTAAA CTGCAACCCT
    AACAACAAAG CCAAGACAGG AAACAAGCCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52893
    Clone ID Related Accession (Same CDS sequence) XM_010810848.2
    Accession Version XM_010810848.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104316.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAAGAGG ATGCAAAACC CAGCAAAGAC AACGAGGACG CCTTTTACAA CTCTCAGAAG 
    TTCGAGGTCC TGTACTGTGG CAGGGTGACC GTGACCCACA AGAAGGCGCC GTCCAGCCTC
    ATCGACGACT GCATCGAGAA GTTCAGCCTG CACGAGCAGC AGCGCTTGCG GCTCCAGAGC
    GAGCCGCGCG CAGGGGACTC GGGCGAAGAC CAGGCGGGAT CCGAGGCCCG AACGCCGGCC
    TGCCCCGTCG ACGTTCTGTC GGAGGAAGGG GATGGCGTCG CCGACACCCA CCCGGTCTTC
    CCTGCCGCGC CCAGCCAGCC TGGGCCATCT AGCTCGCGAG TCTGCTTCCC TGAGAGGATT
    TTGGAAGATT CGGGCTTCGA TGAACAGCAG GAGTTCCGGT CTCGGTGCAG CAGCGTCACG
    GGGGTCCTGC AAAGGAAAGT TCACGAGAAC AGCCAGAAGC CGCAGCCGCG GCGGAGGCAT
    GCCAGCGCGC CCAGCCACGT GCAGCCCTCG GACTCGGAGA AGAACAGAAC CATGCTGTTT
    CAGGTTGGGC GGTTTGAGAT TAACCTTATC AGTCCGGACA CTAAATCAGT TGTGCTTGAG
    AAGAATTTTA AAGATATCTC GTCTTGTTCC CAGGGTATAA AGCATGTTGA TCACTTCGGC
    TTTATTTGTC GGGAGTCCTC GGAGCCTGGA CTGAGCCAGT ATATCTGCTA CGTGTTCCAG
    TGTGCCAGTG AATCTCTGGT TGATGAGGTA ATGCTGACTC TGAAACAGGC TTTCAGCACG
    GCCGCTGCCC TGCAGAGTGC CAAGACCCAG ATTAAGCTGT GTGAGGCCTG CCCCATGCAC
    TCTTTGCATA AGCTCTGTGA AAGGATCGAA GGTCTCTACC CACCAAGAGC CAAGCTGGTA
    ATACAGAGGC ATCTCTCATC CCTGACAGAT AACGAGCAAG CTGACATCTT TGAACGCGTT
    CAGAAAATGA AGGCAGTCAA TGACCAGGAG GAGAATGAAC TTGTGATTTT ACATCTGAGG
    CAGCTATGCG AAGCCAAGCA GAAGACACAT GTTCATATCG GGGAAGGCCC ATTGACTATT
    TCAAATAGTA CAATCCCGGA AAATGCGACA AGCAGTGGAA GGTTCAAACT TGACATTCTG
    AAAAATAAAG CTAAGAGATC CTTAACTAGC TCCCTGGAAA ATATCTTCTC AAGGGGAGCT
    AACAGAATGA GAGGTCGGCT TGGAAGTGTG GACAGCTTTG AACGATCCAA CAGTGTTGCT
    TCAGAGAAGG ACTACTCGCC AGGAGACTCT CCGCCAGGGA CCCCGCCAGC TTCCCCCCTG
    TCCTCAGCTT GGCACACATT CCCTGAGGAG GATTCCGACT CCCCGCAGTT TCGAAGACGG
    GCCCACACAT TCAGCCACCC ACCTTCAAGC ACGAAGAGGA AGCTGAATTT GCAGGATGGG
    AAGGCCCATG GGGTTCGCTC CCCTCTTCTG AGGCAGAGCT CCAGTGAGCA GTGCAGCATC
    CTTCCACCAG CTCGGCGCAT GCACAAGGAG TGTAATTCTT CCTCCAGTCT TCCAAGTCTT
    CACACTTCCT TCTCTGCCCC TTCCTTCACT GCCCCCCCTT TCCTGAAAAG CTTTTACCAG
    AATTCAGGTA GACTATCCCC ACAGTATGAG AATGAAATCA GACAAGACAC TGCTTCAGAA
    TCAAGTGATG GAGAGGGGAG AAAAAGGACC TCGTCCACCT GCAGCAATGA ATCCCTAAAC
    GCTGGAGGAA CCCCCGTCAC TCCTCGCCGA GTTTCCTGGC GGCAGCGCAT CTTCCTCAGG
    GTTGCTTCGC CCATGAGGAA ATCTCCCTCA GCGATGCAGC AGGATGGCCT GGACAGGAGT
    GAGCTGCTGC CACTGTCCCC TCTTGCTCCA ACCATGGAGG AGGAACCTCT AATGATATTC
    ATGTCTGATG AAGATGACCC AGAAAAGGGT GAAGAGAGAA ACAAGTCGGA GGAACTGAGG
    TGTTTGTGGA GAAAAGCTAT ACACCAGCAG ATCCTGTTGC TTCGAATGGA AAAAGAAAAC
    CAGAAACTTG AAGCAAGCAG AGATGAACTG CAGTCCAGAA AAGTTAAATT GGATTATGAA
    GAAGTTGGTG CATGTCAGAA GGAGGTCTTA ACAATCTGGG ATAAGAAGTT GTTAAACTGC
    AGAGCTAAAA TCAGATGCGA TATGGAAGAT ATTCATACTT CTCTTAAAGA AGGTGTTCCC
    AAAAGTCGAC GAGGAGAAAT CTGGCAGTTC CTAGCTTTGC AATATCGTCT GAGACACAGA
    TTGCCTAATA AACAACAACC TCCCGACATA TCCTATAAAG AGCTTTTAAA ACAGCTCACG
    GCTCAGCAGC ATGCTATCCT CGTGGATTTA GGGAGAACAT TTCCTACTCA TCCTTACTTT
    TCAGCACAGC TTGGGGCAGG GCAGCTGTCG CTCTTTAATC TCTTGAAAGC CTACTCATTA
    CTGGACAAAG AAGTGGGTTA CTGTCAGGGG ATCAGCTTTG TGGCTGGAGT CTTGCTTCTA
    CACATGAGTG AAGAGCAAGC CTTTGAAATG CTGAAATTCC TCATGTATGA CCTGGGCTTC
    CGCAAGCAGT ACCGACCTGA CATGATGTCG CTGCAGATTC AAATGTACCA GCTGTCCAGG
    CTCCTTCATG ATTATCACAG AGATCTCTAC AATCATCTTG AAGAAAATGA AATCAGCCCC
    AGTCTTTATG CTGCCCCCTG GTTCCTCACG TTGTTTGCCT CTCAATTTCC ATTAGGATTT
    GTAGCCAGAG TGTTTGATAT TATTTTTCTT CAGGGAACTG AAGTTATATT TAAGGTTGCC
    CTAAGCCTGT TGAGCAACCA AGAGACACTC ATAATGGAAT GTGAAAACTT TGAAAATATT
    GTTGAATTCC TTAAAATCAC TCTCCCCGAT ATGAATTCAT CTGAAATGGA AAAAATAATC
    ACCCAGGTTT TTGAGATGGA TATTTCTAAA CAGTTACATG CCTATGAGGT GGAGTATCAC
    GTGCTACAGG ATGAACTCCA AGAATCTTCC TATGCTTGCG AGGACAGTGA GCCCATAGAG
    AAGCTGGAGA GGGCCAACAG CCAACTGAAA AGACAAAACA TGGACCTTCT AGAAAAATTA
    CAGATAGCTC ATGCTAAAAT TCAGACTTTG GAGTCCAACC TGGAAAATCT TTTAACCAGA
    GAGACCAAAA TGAAGTCTTT GATCCGGACC CTGGAACAGG AGAAGACGGC TTATCAGAAG
    GCAGTGGAGC AGATCTGGAA GCTGCTGCCT GCAGATATTT TAGCCAACTG CGAACCACTG
    CTGAGAGACT TAAACTGCAA CCCTAACAAC AAAGCCAAGA CAGGAAACAA GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010809150.1
    CDS158..3514
    Translation

    Target ORF information:

    RefSeq Version XM_010810848.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810848.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAAGAGG ATGCAAAACC CAGCAAAGAC AACGAGGACG CCTTTTACAA CTCTCAGAAG 
    TTCGAGGTCC TGTACTGTGG CAGGGTGACC GTGACCCACA AGAAGGCGCC GTCCAGCCTC
    ATCGACGACT GCATCGAGAA GTTCAGCCTG CACGAGCAGC AGCGCTTGCG GCTCCAGAGC
    GAGCCGCGCG CAGGGGACTC GGGCGAAGAC CAGGCGGGAT CCGAGGCCCG AACGCCGGCC
    TGCCCCGTCG ACGTTCTGTC GGAGGAAGGG GATGGCGTCG CCGACACCCA CCCGGTCTTC
    CCTGCCGCGC CCAGCCAGCC TGGGCCATCT AGCTCGCGAG TCTGCTTCCC TGAGAGGATT
    TTGGAAGATT CGGGCTTCGA TGAACAGCAG GAGTTCCGGT CTCGGTGCAG CAGCGTCACG
    GGGGTCCTGC AAAGGAAAGT TCACGAGAAC AGCCAGAAGC CGCAGCCGCG GCGGAGGCAT
    GCCAGCGCGC CCAGCCACGT GCAGCCCTCG GACTCGGAGA AGAACAGAAC CATGCTGTTT
    CAGGTTGGGC GGTTTGAGAT TAACCTTATC AGTCCGGACA CTAAATCAGT TGTGCTTGAG
    AAGAATTTTA AAGATATCTC GTCTTGTTCC CAGGGTATAA AGCATGTTGA TCACTTCGGC
    TTTATTTGTC GGGAGTCCTC GGAGCCTGGA CTGAGCCAGT ATATCTGCTA CGTGTTCCAG
    TGTGCCAGTG AATCTCTGGT TGATGAGGTA ATGCTGACTC TGAAACAGGC TTTCAGCACG
    GCCGCTGCCC TGCAGAGTGC CAAGACCCAG ATTAAGCTGT GTGAGGCCTG CCCCATGCAC
    TCTTTGCATA AGCTCTGTGA AAGGATCGAA GGTCTCTACC CACCAAGAGC CAAGCTGGTA
    ATACAGAGGC ATCTCTCATC CCTGACAGAT AACGAGCAAG CTGACATCTT TGAACGCGTT
    CAGAAAATGA AGGCAGTCAA TGACCAGGAG GAGAATGAAC TTGTGATTTT ACATCTGAGG
    CAGCTATGCG AAGCCAAGCA GAAGACACAT GTTCATATCG GGGAAGGCCC ATTGACTATT
    TCAAATAGTA CAATCCCGGA AAATGCGACA AGCAGTGGAA GGTTCAAACT TGACATTCTG
    AAAAATAAAG CTAAGAGATC CTTAACTAGC TCCCTGGAAA ATATCTTCTC AAGGGGAGCT
    AACAGAATGA GAGGTCGGCT TGGAAGTGTG GACAGCTTTG AACGATCCAA CAGTGTTGCT
    TCAGAGAAGG ACTACTCGCC AGGAGACTCT CCGCCAGGGA CCCCGCCAGC TTCCCCCCTG
    TCCTCAGCTT GGCACACATT CCCTGAGGAG GATTCCGACT CCCCGCAGTT TCGAAGACGG
    GCCCACACAT TCAGCCACCC ACCTTCAAGC ACGAAGAGGA AGCTGAATTT GCAGGATGGG
    AAGGCCCATG GGGTTCGCTC CCCTCTTCTG AGGCAGAGCT CCAGTGAGCA GTGCAGCATC
    CTTCCACCAG CTCGGCGCAT GCACAAGGAG TGTAATTCTT CCTCCAGTCT TCCAAGTCTT
    CACACTTCCT TCTCTGCCCC TTCCTTCACT GCCCCCCCTT TCCTGAAAAG CTTTTACCAG
    AATTCAGGTA GACTATCCCC ACAGTATGAG AATGAAATCA GACAAGACAC TGCTTCAGAA
    TCAAGTGATG GAGAGGGGAG AAAAAGGACC TCGTCCACCT GCAGCAATGA ATCCCTAAAC
    GCTGGAGGAA CCCCCGTCAC TCCTCGCCGA GTTTCCTGGC GGCAGCGCAT CTTCCTCAGG
    GTTGCTTCGC CCATGAGGAA ATCTCCCTCA GCGATGCAGC AGGATGGCCT GGACAGGAGT
    GAGCTGCTGC CACTGTCCCC TCTTGCTCCA ACCATGGAGG AGGAACCTCT AATGATATTC
    ATGTCTGATG AAGATGACCC AGAAAAGGGT GAAGAGAGAA ACAAGTCGGA GGAACTGAGG
    TGTTTGTGGA GAAAAGCTAT ACACCAGCAG ATCCTGTTGC TTCGAATGGA AAAAGAAAAC
    CAGAAACTTG AAGCAAGCAG AGATGAACTG CAGTCCAGAA AAGTTAAATT GGATTATGAA
    GAAGTTGGTG CATGTCAGAA GGAGGTCTTA ACAATCTGGG ATAAGAAGTT GTTAAACTGC
    AGAGCTAAAA TCAGATGCGA TATGGAAGAT ATTCATACTT CTCTTAAAGA AGGTGTTCCC
    AAAAGTCGAC GAGGAGAAAT CTGGCAGTTC CTAGCTTTGC AATATCGTCT GAGACACAGA
    TTGCCTAATA AACAACAACC TCCCGACATA TCCTATAAAG AGCTTTTAAA ACAGCTCACG
    GCTCAGCAGC ATGCTATCCT CGTGGATTTA GGGAGAACAT TTCCTACTCA TCCTTACTTT
    TCAGCACAGC TTGGGGCAGG GCAGCTGTCG CTCTTTAATC TCTTGAAAGC CTACTCATTA
    CTGGACAAAG AAGTGGGTTA CTGTCAGGGG ATCAGCTTTG TGGCTGGAGT CTTGCTTCTA
    CACATGAGTG AAGAGCAAGC CTTTGAAATG CTGAAATTCC TCATGTATGA CCTGGGCTTC
    CGCAAGCAGT ACCGACCTGA CATGATGTCG CTGCAGATTC AAATGTACCA GCTGTCCAGG
    CTCCTTCATG ATTATCACAG AGATCTCTAC AATCATCTTG AAGAAAATGA AATCAGCCCC
    AGTCTTTATG CTGCCCCCTG GTTCCTCACG TTGTTTGCCT CTCAATTTCC ATTAGGATTT
    GTAGCCAGAG TGTTTGATAT TATTTTTCTT CAGGGAACTG AAGTTATATT TAAGGTTGCC
    CTAAGCCTGT TGAGCAACCA AGAGACACTC ATAATGGAAT GTGAAAACTT TGAAAATATT
    GTTGAATTCC TTAAAATCAC TCTCCCCGAT ATGAATTCAT CTGAAATGGA AAAAATAATC
    ACCCAGGTTT TTGAGATGGA TATTTCTAAA CAGTTACATG CCTATGAGGT GGAGTATCAC
    GTGCTACAGG ATGAACTCCA AGAATCTTCC TATGCTTGCG AGGACAGTGA GCCCATAGAG
    AAGCTGGAGA GGGCCAACAG CCAACTGAAA AGACAAAACA TGGACCTTCT AGAAAAATTA
    CAGATAGCTC ATGCTAAAAT TCAGACTTTG GAGTCCAACC TGGAAAATCT TTTAACCAGA
    GAGACCAAAA TGAAGTCTTT GATCCGGACC CTGGAACAGG AGAAGACGGC TTATCAGAAG
    GCAGTGGAGC AGATCTGGAA GCTGCTGCCT GCAGATATTT TAGCCAACTG CGAACCACTG
    CTGAGAGACT TAAACTGCAA CCCTAACAAC AAAGCCAAGA CAGGAAACAA GCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216390
    Clone ID Related Accession (Same CDS sequence) NM_001206310.2
    Accession Version NM_001206310.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02033566.1 and DAAA02033560.1. On Jun 30, 2017 this sequence version replaced NM_001206310.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN04019650 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAACGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGACT
    ATTTCAAATA GTACAATCCC GGAAAATGCG ACAAGCAGTG GAAGGTTCAA ACTTGACATT
    CTGAAAAATA AAGCTAAGAG ATCCTTAACT AGCTCCCTGG AAAATATCTT CTCAAGGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC ATTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGC
    ATCCTTCCAC CAGCTCGGCG CATGCACAAG GAGTGTAATT CTTCCTCCAG TCTTCCAAGT
    CTTCACACTT CCTTCTCTGC CCCTTCCTTC ACTGCCCCCC CTTTCCTGAA AAGCTTTTAC
    CAGAATTCAG GTAGACTATC CCCACAGTAT GAGAATGAAA TCAGACAAGA CACTGCTTCA
    GAATCAAGTG ATGGAGAGGG GAGAAAAAGG ACCTCGTCCA CCTGCAGCAA TGAATCCCTA
    AACGCTGGAG GAACCCCCGT CACTCCTCGC CGAGTTTCCT GGCGGCAGCG CATCTTCCTC
    AGGGTTGCTT CGCCCATGAG GAAATCTCCC TCAGCGATGC AGCAGGATGG CCTGGACAGG
    AGTGAGCTGC TGCCACTGTC CCCTCTTGCT CCAACCATGG AGGAGGAACC TCTAATGATA
    TTCATGTCTG ATGAAGATGA CCCAGAAAAG GGTGAAGAGA GAAACAAGTC GGAGGAACTG
    AGGTGTTTGT GGAGAAAAGC TATACACCAG CAGATCCTGT TGCTTCGAAT GGAAAAAGAA
    AACCAGAAAC TTGAAGCAAG CAGAGATGAA CTGCAGTCCA GAAAAGTTAA ATTGGATTAT
    GAAGAAGTTG GTGCATGTCA GAAGGAGGTC TTAACAATCT GGGATAAGAA GTTGTTAAAC
    TGCAGAGCTA AAATCAGATG CGATATGGAA GATATTCATA CTTCTCTTAA AGAAGGTGTT
    CCCAAAAGTC GACGAGGAGA AATCTGGCAG TTCCTAGCTT TGCAATATCG TCTGAGACAC
    AGATTGCCTA ATAAACAACA ACCTCCCGAC ATATCCTATA AAGAGCTTTT AAAACAGCTC
    ACGGCTCAGC AGCATGCTAT CCTCGTGGAT TTAGGGAGAA CATTTCCTAC TCATCCTTAC
    TTTTCAGCAC AGCTTGGGGC AGGGCAGCTG TCGCTCTTTA ATCTCTTGAA AGCCTACTCA
    TTACTGGACA AAGAAGTGGG TTACTGTCAG GGGATCAGCT TTGTGGCTGG AGTCTTGCTT
    CTACACATGA GTGAAGAGCA AGCCTTTGAA ATGCTGAAAT TCCTCATGTA TGACCTGGGC
    TTCCGCAAGC AGTACCGACC TGACATGATG TCGCTGCAGA TTCAAATGTA CCAGCTGTCC
    AGGCTCCTTC ATGATTATCA CAGAGATCTC TACAATCATC TTGAAGAAAA TGAAATCAGC
    CCCAGTCTTT ATGCTGCCCC CTGGTTCCTC ACGTTGTTTG CCTCTCAATT TCCATTAGGA
    TTTGTAGCCA GAGTGTTTGA TATTATTTTT CTTCAGGGAA CTGAAGTTAT ATTTAAGGTT
    GCCCTAAGCC TGTTGAGCAA CCAAGAGACA CTCATAATGG AATGTGAAAA CTTTGAAAAT
    ATTGTTGAAT TCCTTAAAAT CACTCTCCCC GATATGAATT CATCTGAAAT GGAAAAAATA
    ATCACCCAGG TTTTTGAGAT GGATATTTCT AAACAGTTAC ATGCCTATGA GGTGGAGTAT
    CACGTGCTAC AGGATGAACT CCAAGAATCT TCCTATGCTT GCGAGGACAG TGAGCCCATA
    GAGAAGCTGG AGAGGGCCAA CAGCCAACTG AAAAGACAAA ACATGGACCT TCTAGAAAAA
    TTACAGATAG CTCATGCTAA AATTCAGACT TTGGAGTCCA ACCTGGAAAA TCTTTTAACC
    AGAGAGACCA AAATGAAGTC TTTGATCCGG ACCCTGGAAC AGGAGAAGAC GGCTTATCAG
    AAGGCAGTGG AGCAGATCTG GAAGCTGCTG CCTGCAGATA TTTTAGCCAA CTGCGAACCA
    CTGCTGAGAG ACTTAAACTG CAACCCTAAC AACAAAGCCA AGACAGGAAA CAAGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193239.1
    CDS1..3900
    Translation

    Target ORF information:

    RefSeq Version NM_001206310.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 4 (TBC1D4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206310.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAACGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGACT
    ATTTCAAATA GTACAATCCC GGAAAATGCG ACAAGCAGTG GAAGGTTCAA ACTTGACATT
    CTGAAAAATA AAGCTAAGAG ATCCTTAACT AGCTCCCTGG AAAATATCTT CTCAAGGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC ATTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGC
    ATCCTTCCAC CAGCTCGGCG CATGCACAAG GAGTGTAATT CTTCCTCCAG TCTTCCAAGT
    CTTCACACTT CCTTCTCTGC CCCTTCCTTC ACTGCCCCCC CTTTCCTGAA AAGCTTTTAC
    CAGAATTCAG GTAGACTATC CCCACAGTAT GAGAATGAAA TCAGACAAGA CACTGCTTCA
    GAATCAAGTG ATGGAGAGGG GAGAAAAAGG ACCTCGTCCA CCTGCAGCAA TGAATCCCTA
    AACGCTGGAG GAACCCCCGT CACTCCTCGC CGAGTTTCCT GGCGGCAGCG CATCTTCCTC
    AGGGTTGCTT CGCCCATGAG GAAATCTCCC TCAGCGATGC AGCAGGATGG CCTGGACAGG
    AGTGAGCTGC TGCCACTGTC CCCTCTTGCT CCAACCATGG AGGAGGAACC TCTAATGATA
    TTCATGTCTG ATGAAGATGA CCCAGAAAAG GGTGAAGAGA GAAACAAGTC GGAGGAACTG
    AGGTGTTTGT GGAGAAAAGC TATACACCAG CAGATCCTGT TGCTTCGAAT GGAAAAAGAA
    AACCAGAAAC TTGAAGCAAG CAGAGATGAA CTGCAGTCCA GAAAAGTTAA ATTGGATTAT
    GAAGAAGTTG GTGCATGTCA GAAGGAGGTC TTAACAATCT GGGATAAGAA GTTGTTAAAC
    TGCAGAGCTA AAATCAGATG CGATATGGAA GATATTCATA CTTCTCTTAA AGAAGGTGTT
    CCCAAAAGTC GACGAGGAGA AATCTGGCAG TTCCTAGCTT TGCAATATCG TCTGAGACAC
    AGATTGCCTA ATAAACAACA ACCTCCCGAC ATATCCTATA AAGAGCTTTT AAAACAGCTC
    ACGGCTCAGC AGCATGCTAT CCTCGTGGAT TTAGGGAGAA CATTTCCTAC TCATCCTTAC
    TTTTCAGCAC AGCTTGGGGC AGGGCAGCTG TCGCTCTTTA ATCTCTTGAA AGCCTACTCA
    TTACTGGACA AAGAAGTGGG TTACTGTCAG GGGATCAGCT TTGTGGCTGG AGTCTTGCTT
    CTACACATGA GTGAAGAGCA AGCCTTTGAA ATGCTGAAAT TCCTCATGTA TGACCTGGGC
    TTCCGCAAGC AGTACCGACC TGACATGATG TCGCTGCAGA TTCAAATGTA CCAGCTGTCC
    AGGCTCCTTC ATGATTATCA CAGAGATCTC TACAATCATC TTGAAGAAAA TGAAATCAGC
    CCCAGTCTTT ATGCTGCCCC CTGGTTCCTC ACGTTGTTTG CCTCTCAATT TCCATTAGGA
    TTTGTAGCCA GAGTGTTTGA TATTATTTTT CTTCAGGGAA CTGAAGTTAT ATTTAAGGTT
    GCCCTAAGCC TGTTGAGCAA CCAAGAGACA CTCATAATGG AATGTGAAAA CTTTGAAAAT
    ATTGTTGAAT TCCTTAAAAT CACTCTCCCC GATATGAATT CATCTGAAAT GGAAAAAATA
    ATCACCCAGG TTTTTGAGAT GGATATTTCT AAACAGTTAC ATGCCTATGA GGTGGAGTAT
    CACGTGCTAC AGGATGAACT CCAAGAATCT TCCTATGCTT GCGAGGACAG TGAGCCCATA
    GAGAAGCTGG AGAGGGCCAA CAGCCAACTG AAAAGACAAA ACATGGACCT TCTAGAAAAA
    TTACAGATAG CTCATGCTAA AATTCAGACT TTGGAGTCCA ACCTGGAAAA TCTTTTAACC
    AGAGAGACCA AAATGAAGTC TTTGATCCGG ACCCTGGAAC AGGAGAAGAC GGCTTATCAG
    AAGGCAGTGG AGCAGATCTG GAAGCTGCTG CCTGCAGATA TTTTAGCCAA CTGCGAACCA
    CTGCTGAGAG ACTTAAACTG CAACCCTAAC AACAAAGCCA AGACAGGAAA CAAGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40613
    Clone ID Related Accession (Same CDS sequence) NM_001206310.1
    Accession Version NM_001206310.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product TBC1 domain family member 4
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03121191.1, AAFC03034019.1, AAFC03093646.1 and AAFC03093647.1. On Apr 20, 2011 this sequence version replaced XM_874885.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCTTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAATGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGACT
    ATTTCAAATA GTACAATCCC GGAAAATGCG ACAAGCAGTG GAAGGTTCAA ACTTGACATT
    CTGAAAAATA AAGCTAAGAG ATCCTTAACT AGCTCCCTGG AAAATATCTT CTCAAGGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC GTTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGC
    ATCCTTCCAC CAGCTCGGCG CATGCACAAG GAGTGTAATT CTTCCTCCAG TCTTCCAAGT
    CTTCACACTT CCTTCTCTGC CCCTTCCTTC ACTGCCCCCC CTTTCCTGAA AAGCTTTTAC
    CAGAATTCAG GTAGACTATC CCCACAGTAT GAGAATGAAA TCAGACAAGA CACTGCTTCA
    GAATCAAGTG ATGGAGAGGG GAGAAAAAGG ACCTCGTCCA CCTGCAGCAA TGAATCCCTA
    AACGCTGGAG GAACCCCCGT CACTCCTCGC CGAGTTTCCT GGCGGCAGCG CATCTTCCTC
    AGGGTTGCTT CGCCCATGAG GAAATCTCCC TCAGCGATGC AGCAGGATGG CCTGGACAGG
    AGTGAGCTGC TGCCACTGTC CCCTCTCGCT CCAACCATGG AGGAGGAACC TCTGATGATA
    TTCATGTCTG ATGAAGATGA CCCAGAAAAG GGTGAAGAGA GAAACAAGTC GGAGGAACTG
    AGGTGTTTGT GGAGAAAAGC TATACACCAG CAGATCCTGT TGCTTCGAAT GGAAAAAGAA
    AACCAGAAAC TTGAAGCAAG CAGAGATGAA CTGCAGTCCA GAAAAGTTAA ATTGGATTAT
    GAAGAAGTTG GTGCATGTCA GAAGGAGGTC TTAACAATCT GGGATAAGAA GTTGTTAAAC
    TGCAGAGCTA AAATCAGATG CGATATGGAA GATATTCATA CTTCTCTTAA AGAAGGTGTT
    CCCAAAAGTC GACGAGGAGA AATCTGGCAG TTCCTAGCTT TGCAATATCG TCTGAGACAC
    AGATTGCCTA ATAAACAACA ACCTCCCGAC ATATCCTATA AAGAGCTTTT AAAACAGCTC
    ACGGCTCAGC AGCATGCTAT CCTCGTGGAT TTAGGGAGAA CATTTCCTAC TCATCCTTAC
    TTTTCAGCAC AGCTTGGGGC AGGGCAGCTG TCGCTCTTTA ATCTCTTGAA AGCCTACTCA
    TTACTGGACA AAGAAGTGGG TTACTGTCAG GGGATCAGCT TTGTGGCTGG AGTCTTGCTT
    CTACACATGA GTGAAGAGCA AGCCTTTGAA ATGCTGAAAT TCCTCATGTA TGACCTGGGC
    TTCCGCAAGC AGTACCGACC TGACATGATG TCGCTGCAGA TTCAAATGTA CCAGCTGTCC
    AGGCTCCTTC ATGATTATCA CAGAGATCTC TACAATCATC TTGAAGAAAA TGAAATCAGC
    CCCAGTCTTT ATGCTGCCCC CTGGTTCCTC ACGTTGTTTG CCTCTCAATT TCCATTAGGA
    TTTGTAGCCA GAGTGTTTGA TATTATTTTT CTTCAGGGAA CTGAAGTTAT ATTTAAGGTT
    GCCCTAAGCC TGTTGAGCAA CCAAGAGACA CTCATAATGG AATGTGAAAA CTTTGAAAAT
    ATTGTTGAAT TCCTTAAAAT CACTCTCCCC GATATGAATT CATCTGAAAT GGAAAAAATA
    ATCACCCAGG TTTTTGAGAT GGATATTTCT AAACAGTTAC ATGCCTATGA GGTGGAGTAT
    CACGTGCTAC AGGATGAACT CCAAGAATCT TCCTATGCTT GCGAGGACAG TGAGCCCATA
    GAGAAGCTGG AGAGGGCCAA CAGCCAACTG AAAAGACAAA ACATGGACCT TCTAGAAAAA
    TTACAGATAG CTCATGCTAA AATTCAGACT TTGGAGTCCA ACCTGGAAAA TCTTTTAACC
    AGAGAGACCA AAATGAAGTC TTTGATCCGG ACCCTGGAAC AGGAGAAGAC GGCTTATCAG
    AAGGCAGTGG AGCAGATCTG GAAGCTGCTG CCTGCAGATA TTTTAGCCAA CTGCGAACCA
    CTGCTGAGAG ACTTAAACTG CAACCCTAAC AACAAAGCCA AGACAGGAAA CAAGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193239.1
    CDS1..3900
    Translation

    Target ORF information:

    RefSeq Version NM_001206310.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus TBC1 domain family, member 4 (TBC1D4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206310.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCTTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAATGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGACT
    ATTTCAAATA GTACAATCCC GGAAAATGCG ACAAGCAGTG GAAGGTTCAA ACTTGACATT
    CTGAAAAATA AAGCTAAGAG ATCCTTAACT AGCTCCCTGG AAAATATCTT CTCAAGGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC GTTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGC
    ATCCTTCCAC CAGCTCGGCG CATGCACAAG GAGTGTAATT CTTCCTCCAG TCTTCCAAGT
    CTTCACACTT CCTTCTCTGC CCCTTCCTTC ACTGCCCCCC CTTTCCTGAA AAGCTTTTAC
    CAGAATTCAG GTAGACTATC CCCACAGTAT GAGAATGAAA TCAGACAAGA CACTGCTTCA
    GAATCAAGTG ATGGAGAGGG GAGAAAAAGG ACCTCGTCCA CCTGCAGCAA TGAATCCCTA
    AACGCTGGAG GAACCCCCGT CACTCCTCGC CGAGTTTCCT GGCGGCAGCG CATCTTCCTC
    AGGGTTGCTT CGCCCATGAG GAAATCTCCC TCAGCGATGC AGCAGGATGG CCTGGACAGG
    AGTGAGCTGC TGCCACTGTC CCCTCTCGCT CCAACCATGG AGGAGGAACC TCTGATGATA
    TTCATGTCTG ATGAAGATGA CCCAGAAAAG GGTGAAGAGA GAAACAAGTC GGAGGAACTG
    AGGTGTTTGT GGAGAAAAGC TATACACCAG CAGATCCTGT TGCTTCGAAT GGAAAAAGAA
    AACCAGAAAC TTGAAGCAAG CAGAGATGAA CTGCAGTCCA GAAAAGTTAA ATTGGATTAT
    GAAGAAGTTG GTGCATGTCA GAAGGAGGTC TTAACAATCT GGGATAAGAA GTTGTTAAAC
    TGCAGAGCTA AAATCAGATG CGATATGGAA GATATTCATA CTTCTCTTAA AGAAGGTGTT
    CCCAAAAGTC GACGAGGAGA AATCTGGCAG TTCCTAGCTT TGCAATATCG TCTGAGACAC
    AGATTGCCTA ATAAACAACA ACCTCCCGAC ATATCCTATA AAGAGCTTTT AAAACAGCTC
    ACGGCTCAGC AGCATGCTAT CCTCGTGGAT TTAGGGAGAA CATTTCCTAC TCATCCTTAC
    TTTTCAGCAC AGCTTGGGGC AGGGCAGCTG TCGCTCTTTA ATCTCTTGAA AGCCTACTCA
    TTACTGGACA AAGAAGTGGG TTACTGTCAG GGGATCAGCT TTGTGGCTGG AGTCTTGCTT
    CTACACATGA GTGAAGAGCA AGCCTTTGAA ATGCTGAAAT TCCTCATGTA TGACCTGGGC
    TTCCGCAAGC AGTACCGACC TGACATGATG TCGCTGCAGA TTCAAATGTA CCAGCTGTCC
    AGGCTCCTTC ATGATTATCA CAGAGATCTC TACAATCATC TTGAAGAAAA TGAAATCAGC
    CCCAGTCTTT ATGCTGCCCC CTGGTTCCTC ACGTTGTTTG CCTCTCAATT TCCATTAGGA
    TTTGTAGCCA GAGTGTTTGA TATTATTTTT CTTCAGGGAA CTGAAGTTAT ATTTAAGGTT
    GCCCTAAGCC TGTTGAGCAA CCAAGAGACA CTCATAATGG AATGTGAAAA CTTTGAAAAT
    ATTGTTGAAT TCCTTAAAAT CACTCTCCCC GATATGAATT CATCTGAAAT GGAAAAAATA
    ATCACCCAGG TTTTTGAGAT GGATATTTCT AAACAGTTAC ATGCCTATGA GGTGGAGTAT
    CACGTGCTAC AGGATGAACT CCAAGAATCT TCCTATGCTT GCGAGGACAG TGAGCCCATA
    GAGAAGCTGG AGAGGGCCAA CAGCCAACTG AAAAGACAAA ACATGGACCT TCTAGAAAAA
    TTACAGATAG CTCATGCTAA AATTCAGACT TTGGAGTCCA ACCTGGAAAA TCTTTTAACC
    AGAGAGACCA AAATGAAGTC TTTGATCCGG ACCCTGGAAC AGGAGAAGAC GGCTTATCAG
    AAGGCAGTGG AGCAGATCTG GAAGCTGCTG CCTGCAGATA TTTTAGCCAA CTGCGAACCA
    CTGCTGAGAG ACTTAAACTG CAACCCTAAC AACAAAGCCA AGACAGGAAA CAAGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24805
    Clone ID Related Accession (Same CDS sequence) XM_005213844.3
    Accession Version XM_005213844.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104316.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213844.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAACGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGACT
    ATTTCAAATA GTACAATCCC GGAAAATGCG ACAAGCAGTG GAAGGTTCAA ACTTGACATT
    CTGAAAAATA AAGCTAAGAG ATCCTTAACT AGCTCCCTGG AAAATATCTT CTCAAGGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC ATTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGC
    ATCCTTCCAC CAGCTCGGCG CATGCACAAG GAGTGTAATT CTTCCTCCAG TCTTCCAAGT
    CTTCACACTT CCTTCTCTGC CCCTTCCTTC ACTGCCCCCC CTTTCCTGAA AAGCTTTTAC
    CAGAATTCAG GTAGACTATC CCCACAGTAT GAGAATGAAA TCAGTGATGG AGAGGGGAGA
    AAAAGGACCT CGTCCACCTG CAGCAATGAA TCCCTAAACG CTGGAGGAAC CCCCGTCACT
    CCTCGCCGAG TTTCCTGGCG GCAGCGCATC TTCCTCAGGG TTGCTTCGCC CATGAGGAAA
    TCTCCCTCAG CGATGCAGCA GGATGGCCTG GACAGGAGTG AGCTGCTGCC ACTGTCCCCT
    CTTGCTCCAA CCATGGAGGA GGAACCTCTA ATGATATTCA TGTCTGATGA AGATGACCCA
    GAAAAGGGTG AAGAGAGAAA CAAGTCGGAG GAACTGAGGT GTTTGTGGAG AAAAGCTATA
    CACCAGCAGA TCCTGTTGCT TCGAATGGAA AAAGAAAACC AGAAACTTGA AGCAAGCAGA
    GATGAACTGC AGTCCAGAAA AGTTAAATTG GATTATGAAG AAGTTGGTGC ATGTCAGAAG
    GAGGTCTTAA CAATCTGGGA TAAGAAGTTG TTAAACTGCA GAGCTAAAAT CAGATGCGAT
    ATGGAAGATA TTCATACTTC TCTTAAAGAA GGTGTTCCCA AAAGTCGACG AGGAGAAATC
    TGGCAGTTCC TAGCTTTGCA ATATCGTCTG AGACACAGAT TGCCTAATAA ACAACAACCT
    CCCGACATAT CCTATAAAGA GCTTTTAAAA CAGCTCACGG CTCAGCAGCA TGCTATCCTC
    GTGGATTTAG GGAGAACATT TCCTACTCAT CCTTACTTTT CAGCACAGCT TGGGGCAGGG
    CAGCTGTCGC TCTTTAATCT CTTGAAAGCC TACTCATTAC TGGACAAAGA AGTGGGTTAC
    TGTCAGGGGA TCAGCTTTGT GGCTGGAGTC TTGCTTCTAC ACATGAGTGA AGAGCAAGCC
    TTTGAAATGC TGAAATTCCT CATGTATGAC CTGGGCTTCC GCAAGCAGTA CCGACCTGAC
    ATGATGTCGC TGCAGATTCA AATGTACCAG CTGTCCAGGC TCCTTCATGA TTATCACAGA
    GATCTCTACA ATCATCTTGA AGAAAATGAA ATCAGCCCCA GTCTTTATGC TGCCCCCTGG
    TTCCTCACGT TGTTTGCCTC TCAATTTCCA TTAGGATTTG TAGCCAGAGT GTTTGATATT
    ATTTTTCTTC AGGGAACTGA AGTTATATTT AAGGTTGCCC TAAGCCTGTT GAGCAACCAA
    GAGACACTCA TAATGGAATG TGAAAACTTT GAAAATATTG TTGAATTCCT TAAAATCACT
    CTCCCCGATA TGAATTCATC TGAAATGGAA AAAATAATCA CCCAGGTTTT TGAGATGGAT
    ATTTCTAAAC AGTTACATGC CTATGAGGTG GAGTATCACG TGCTACAGGA TGAACTCCAA
    GAATCTTCCT ATGCTTGCGA GGACAGTGAG CCCATAGAGA AGCTGGAGAG GGCCAACAGC
    CAACTGAAAA GACAAAACAT GGACCTTCTA GAAAAATTAC AGATAGCTCA TGCTAAAATT
    CAGACTTTGG AGTCCAACCT GGAAAATCTT TTAACCAGAG AGACCAAAAT GAAGTCTTTG
    ATCCGGACCC TGGAACAGGA GAAGACGGCT TATCAGAAGG CAGTGGAGCA GATCTGGAAG
    CTGCTGCCTG CAGATATTTT AGCCAACTGC GAACCACTGC TGAGAGACTT AAACTGCAAC
    CCTAACAACA AAGCCAAGAC AGGAAACAAG CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213901.1
    CDS112..3987
    Translation

    Target ORF information:

    RefSeq Version XM_005213844.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213844.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAACGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGACT
    ATTTCAAATA GTACAATCCC GGAAAATGCG ACAAGCAGTG GAAGGTTCAA ACTTGACATT
    CTGAAAAATA AAGCTAAGAG ATCCTTAACT AGCTCCCTGG AAAATATCTT CTCAAGGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC ATTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGC
    ATCCTTCCAC CAGCTCGGCG CATGCACAAG GAGTGTAATT CTTCCTCCAG TCTTCCAAGT
    CTTCACACTT CCTTCTCTGC CCCTTCCTTC ACTGCCCCCC CTTTCCTGAA AAGCTTTTAC
    CAGAATTCAG GTAGACTATC CCCACAGTAT GAGAATGAAA TCAGTGATGG AGAGGGGAGA
    AAAAGGACCT CGTCCACCTG CAGCAATGAA TCCCTAAACG CTGGAGGAAC CCCCGTCACT
    CCTCGCCGAG TTTCCTGGCG GCAGCGCATC TTCCTCAGGG TTGCTTCGCC CATGAGGAAA
    TCTCCCTCAG CGATGCAGCA GGATGGCCTG GACAGGAGTG AGCTGCTGCC ACTGTCCCCT
    CTTGCTCCAA CCATGGAGGA GGAACCTCTA ATGATATTCA TGTCTGATGA AGATGACCCA
    GAAAAGGGTG AAGAGAGAAA CAAGTCGGAG GAACTGAGGT GTTTGTGGAG AAAAGCTATA
    CACCAGCAGA TCCTGTTGCT TCGAATGGAA AAAGAAAACC AGAAACTTGA AGCAAGCAGA
    GATGAACTGC AGTCCAGAAA AGTTAAATTG GATTATGAAG AAGTTGGTGC ATGTCAGAAG
    GAGGTCTTAA CAATCTGGGA TAAGAAGTTG TTAAACTGCA GAGCTAAAAT CAGATGCGAT
    ATGGAAGATA TTCATACTTC TCTTAAAGAA GGTGTTCCCA AAAGTCGACG AGGAGAAATC
    TGGCAGTTCC TAGCTTTGCA ATATCGTCTG AGACACAGAT TGCCTAATAA ACAACAACCT
    CCCGACATAT CCTATAAAGA GCTTTTAAAA CAGCTCACGG CTCAGCAGCA TGCTATCCTC
    GTGGATTTAG GGAGAACATT TCCTACTCAT CCTTACTTTT CAGCACAGCT TGGGGCAGGG
    CAGCTGTCGC TCTTTAATCT CTTGAAAGCC TACTCATTAC TGGACAAAGA AGTGGGTTAC
    TGTCAGGGGA TCAGCTTTGT GGCTGGAGTC TTGCTTCTAC ACATGAGTGA AGAGCAAGCC
    TTTGAAATGC TGAAATTCCT CATGTATGAC CTGGGCTTCC GCAAGCAGTA CCGACCTGAC
    ATGATGTCGC TGCAGATTCA AATGTACCAG CTGTCCAGGC TCCTTCATGA TTATCACAGA
    GATCTCTACA ATCATCTTGA AGAAAATGAA ATCAGCCCCA GTCTTTATGC TGCCCCCTGG
    TTCCTCACGT TGTTTGCCTC TCAATTTCCA TTAGGATTTG TAGCCAGAGT GTTTGATATT
    ATTTTTCTTC AGGGAACTGA AGTTATATTT AAGGTTGCCC TAAGCCTGTT GAGCAACCAA
    GAGACACTCA TAATGGAATG TGAAAACTTT GAAAATATTG TTGAATTCCT TAAAATCACT
    CTCCCCGATA TGAATTCATC TGAAATGGAA AAAATAATCA CCCAGGTTTT TGAGATGGAT
    ATTTCTAAAC AGTTACATGC CTATGAGGTG GAGTATCACG TGCTACAGGA TGAACTCCAA
    GAATCTTCCT ATGCTTGCGA GGACAGTGAG CCCATAGAGA AGCTGGAGAG GGCCAACAGC
    CAACTGAAAA GACAAAACAT GGACCTTCTA GAAAAATTAC AGATAGCTCA TGCTAAAATT
    CAGACTTTGG AGTCCAACCT GGAAAATCTT TTAACCAGAG AGACCAAAAT GAAGTCTTTG
    ATCCGGACCC TGGAACAGGA GAAGACGGCT TATCAGAAGG CAGTGGAGCA GATCTGGAAG
    CTGCTGCCTG CAGATATTTT AGCCAACTGC GAACCACTGC TGAGAGACTT AAACTGCAAC
    CCTAACAACA AAGCCAAGAC AGGAAACAAG CCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24808
    Clone ID Related Accession (Same CDS sequence) XM_005213847.3
    Accession Version XM_005213847.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104316.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213847.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAACGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGACT
    ATTTCAAATA GTACAATCCC GGAAAATGCG ACAAGCAGTG GAAGGTTCAA ACTTGACATT
    CTGAAAAATA AAGCTAAGAG ATCCTTAACT AGCTCCCTGG AAAATATCTT CTCAAGGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC ATTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGT
    GATGGAGAGG GGAGAAAAAG GACCTCGTCC ACCTGCAGCA ATGAATCCCT AAACGCTGGA
    GGAACCCCCG TCACTCCTCG CCGAGTTTCC TGGCGGCAGC GCATCTTCCT CAGGGTTGCT
    TCGCCCATGA GGAAATCTCC CTCAGCGATG CAGCAGGATG GCCTGGACAG GAGTGAGCTG
    CTGCCACTGT CCCCTCTTGC TCCAACCATG GAGGAGGAAC CTCTAATGAT ATTCATGTCT
    GATGAAGATG ACCCAGAAAA GGGTGAAGAG AGAAACAAGT CGGAGGAACT GAGGTGTTTG
    TGGAGAAAAG CTATACACCA GCAGATCCTG TTGCTTCGAA TGGAAAAAGA AAACCAGAAA
    CTTGAAGCAA GCAGAGATGA ACTGCAGTCC AGAAAAGTTA AATTGGATTA TGAAGAAGTT
    GGTGCATGTC AGAAGGAGGT CTTAACAATC TGGGATAAGA AGTTGTTAAA CTGCAGAGCT
    AAAATCAGAT GCGATATGGA AGATATTCAT ACTTCTCTTA AAGAAGGTGT TCCCAAAAGT
    CGACGAGGAG AAATCTGGCA GTTCCTAGCT TTGCAATATC GTCTGAGACA CAGATTGCCT
    AATAAACAAC AACCTCCCGA CATATCCTAT AAAGAGCTTT TAAAACAGCT CACGGCTCAG
    CAGCATGCTA TCCTCGTGGA TTTAGGGAGA ACATTTCCTA CTCATCCTTA CTTTTCAGCA
    CAGCTTGGGG CAGGGCAGCT GTCGCTCTTT AATCTCTTGA AAGCCTACTC ATTACTGGAC
    AAAGAAGTGG GTTACTGTCA GGGGATCAGC TTTGTGGCTG GAGTCTTGCT TCTACACATG
    AGTGAAGAGC AAGCCTTTGA AATGCTGAAA TTCCTCATGT ATGACCTGGG CTTCCGCAAG
    CAGTACCGAC CTGACATGAT GTCGCTGCAG ATTCAAATGT ACCAGCTGTC CAGGCTCCTT
    CATGATTATC ACAGAGATCT CTACAATCAT CTTGAAGAAA ATGAAATCAG CCCCAGTCTT
    TATGCTGCCC CCTGGTTCCT CACGTTGTTT GCCTCTCAAT TTCCATTAGG ATTTGTAGCC
    AGAGTGTTTG ATATTATTTT TCTTCAGGGA ACTGAAGTTA TATTTAAGGT TGCCCTAAGC
    CTGTTGAGCA ACCAAGAGAC ACTCATAATG GAATGTGAAA ACTTTGAAAA TATTGTTGAA
    TTCCTTAAAA TCACTCTCCC CGATATGAAT TCATCTGAAA TGGAAAAAAT AATCACCCAG
    GTTTTTGAGA TGGATATTTC TAAACAGTTA CATGCCTATG AGGTGGAGTA TCACGTGCTA
    CAGGATGAAC TCCAAGAATC TTCCTATGCT TGCGAGGACA GTGAGCCCAT AGAGAAGCTG
    GAGAGGGCCA ACAGCCAACT GAAAAGACAA AACATGGACC TTCTAGAAAA ATTACAGATA
    GCTCATGCTA AAATTCAGAC TTTGGAGTCC AACCTGGAAA ATCTTTTAAC CAGAGAGACC
    AAAATGAAGT CTTTGATCCG GACCCTGGAA CAGGAGAAGA CGGCTTATCA GAAGGCAGTG
    GAGCAGATCT GGAAGCTGCT GCCTGCAGAT ATTTTAGCCA ACTGCGAACC ACTGCTGAGA
    GACTTAAACT GCAACCCTAA CAACAAAGCC AAGACAGGAA ACAAGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213904.1
    CDS111..3821
    Translation

    Target ORF information:

    RefSeq Version XM_005213847.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213847.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAACGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGACT
    ATTTCAAATA GTACAATCCC GGAAAATGCG ACAAGCAGTG GAAGGTTCAA ACTTGACATT
    CTGAAAAATA AAGCTAAGAG ATCCTTAACT AGCTCCCTGG AAAATATCTT CTCAAGGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC ATTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGT
    GATGGAGAGG GGAGAAAAAG GACCTCGTCC ACCTGCAGCA ATGAATCCCT AAACGCTGGA
    GGAACCCCCG TCACTCCTCG CCGAGTTTCC TGGCGGCAGC GCATCTTCCT CAGGGTTGCT
    TCGCCCATGA GGAAATCTCC CTCAGCGATG CAGCAGGATG GCCTGGACAG GAGTGAGCTG
    CTGCCACTGT CCCCTCTTGC TCCAACCATG GAGGAGGAAC CTCTAATGAT ATTCATGTCT
    GATGAAGATG ACCCAGAAAA GGGTGAAGAG AGAAACAAGT CGGAGGAACT GAGGTGTTTG
    TGGAGAAAAG CTATACACCA GCAGATCCTG TTGCTTCGAA TGGAAAAAGA AAACCAGAAA
    CTTGAAGCAA GCAGAGATGA ACTGCAGTCC AGAAAAGTTA AATTGGATTA TGAAGAAGTT
    GGTGCATGTC AGAAGGAGGT CTTAACAATC TGGGATAAGA AGTTGTTAAA CTGCAGAGCT
    AAAATCAGAT GCGATATGGA AGATATTCAT ACTTCTCTTA AAGAAGGTGT TCCCAAAAGT
    CGACGAGGAG AAATCTGGCA GTTCCTAGCT TTGCAATATC GTCTGAGACA CAGATTGCCT
    AATAAACAAC AACCTCCCGA CATATCCTAT AAAGAGCTTT TAAAACAGCT CACGGCTCAG
    CAGCATGCTA TCCTCGTGGA TTTAGGGAGA ACATTTCCTA CTCATCCTTA CTTTTCAGCA
    CAGCTTGGGG CAGGGCAGCT GTCGCTCTTT AATCTCTTGA AAGCCTACTC ATTACTGGAC
    AAAGAAGTGG GTTACTGTCA GGGGATCAGC TTTGTGGCTG GAGTCTTGCT TCTACACATG
    AGTGAAGAGC AAGCCTTTGA AATGCTGAAA TTCCTCATGT ATGACCTGGG CTTCCGCAAG
    CAGTACCGAC CTGACATGAT GTCGCTGCAG ATTCAAATGT ACCAGCTGTC CAGGCTCCTT
    CATGATTATC ACAGAGATCT CTACAATCAT CTTGAAGAAA ATGAAATCAG CCCCAGTCTT
    TATGCTGCCC CCTGGTTCCT CACGTTGTTT GCCTCTCAAT TTCCATTAGG ATTTGTAGCC
    AGAGTGTTTG ATATTATTTT TCTTCAGGGA ACTGAAGTTA TATTTAAGGT TGCCCTAAGC
    CTGTTGAGCA ACCAAGAGAC ACTCATAATG GAATGTGAAA ACTTTGAAAA TATTGTTGAA
    TTCCTTAAAA TCACTCTCCC CGATATGAAT TCATCTGAAA TGGAAAAAAT AATCACCCAG
    GTTTTTGAGA TGGATATTTC TAAACAGTTA CATGCCTATG AGGTGGAGTA TCACGTGCTA
    CAGGATGAAC TCCAAGAATC TTCCTATGCT TGCGAGGACA GTGAGCCCAT AGAGAAGCTG
    GAGAGGGCCA ACAGCCAACT GAAAAGACAA AACATGGACC TTCTAGAAAA ATTACAGATA
    GCTCATGCTA AAATTCAGAC TTTGGAGTCC AACCTGGAAA ATCTTTTAAC CAGAGAGACC
    AAAATGAAGT CTTTGATCCG GACCCTGGAA CAGGAGAAGA CGGCTTATCA GAAGGCAGTG
    GAGCAGATCT GGAAGCTGCT GCCTGCAGAT ATTTTAGCCA ACTGCGAACC ACTGCTGAGA
    GACTTAAACT GCAACCCTAA CAACAAAGCC AAGACAGGAA ACAAGCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52891
    Clone ID Related Accession (Same CDS sequence) XM_010810846.2
    Accession Version XM_010810846.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104316.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810846.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAACGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC ATTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGC
    ATCCTTCCAC CAGCTCGGCG CATGCACAAG GAGTGTAATT CTTCCTCCAG TCTTCCAAGT
    CTTCACACTT CCTTCTCTGC CCCTTCCTTC ACTGCCCCCC CTTTCCTGAA AAGCTTTTAC
    CAGAATTCAG GTAGACTATC CCCACAGTAT GAGAATGAAA TCAGACAAGA CACTGCTTCA
    GAATCAAGTG ATGGAGAGGG GAGAAAAAGG ACCTCGTCCA CCTGCAGCAA TGAATCCCTA
    AACGCTGGAG GAACCCCCGT CACTCCTCGC CGAGTTTCCT GGCGGCAGCG CATCTTCCTC
    AGGGTTGCTT CGCCCATGAG GAAATCTCCC TCAGCGATGC AGCAGGATGG CCTGGACAGG
    AGTGAGCTGC TGCCACTGTC CCCTCTTGCT CCAACCATGG AGGAGGAACC TCTAATGATA
    TTCATGTCTG ATGAAGATGA CCCAGAAAAG GGTGAAGAGA GAAACAAGTC GGAGGAACTG
    AGGTGTTTGT GGAGAAAAGC TATACACCAG CAGATCCTGT TGCTTCGAAT GGAAAAAGAA
    AACCAGAAAC TTGAAGCAAG CAGAGATGAA CTGCAGTCCA GAAAAGTTAA ATTGGATTAT
    GAAGAAGTTG GTGCATGTCA GAAGGAGGTC TTAACAATCT GGGATAAGAA GTTGTTAAAC
    TGCAGAGCTA AAATCAGATG CGATATGGAA GATATTCATA CTTCTCTTAA AGAAGGTGTT
    CCCAAAAGTC GACGAGGAGA AATCTGGCAG TTCCTAGCTT TGCAATATCG TCTGAGACAC
    AGATTGCCTA ATAAACAACA ACCTCCCGAC ATATCCTATA AAGAGCTTTT AAAACAGCTC
    ACGGCTCAGC AGCATGCTAT CCTCGTGGAT TTAGGGAGAA CATTTCCTAC TCATCCTTAC
    TTTTCAGCAC AGCTTGGGGC AGGGCAGCTG TCGCTCTTTA ATCTCTTGAA AGCCTACTCA
    TTACTGGACA AAGAAGTGGG TTACTGTCAG GGGATCAGCT TTGTGGCTGG AGTCTTGCTT
    CTACACATGA GTGAAGAGCA AGCCTTTGAA ATGCTGAAAT TCCTCATGTA TGACCTGGGC
    TTCCGCAAGC AGTACCGACC TGACATGATG TCGCTGCAGA TTCAAATGTA CCAGCTGTCC
    AGGCTCCTTC ATGATTATCA CAGAGATCTC TACAATCATC TTGAAGAAAA TGAAATCAGC
    CCCAGTCTTT ATGCTGCCCC CTGGTTCCTC ACGTTGTTTG CCTCTCAATT TCCATTAGGA
    TTTGTAGCCA GAGTGTTTGA TATTATTTTT CTTCAGGGAA CTGAAGTTAT ATTTAAGGTT
    GCCCTAAGCC TGTTGAGCAA CCAAGAGACA CTCATAATGG AATGTGAAAA CTTTGAAAAT
    ATTGTTGAAT TCCTTAAAAT CACTCTCCCC GATATGAATT CATCTGAAAT GGAAAAAATA
    ATCACCCAGG TTTTTGAGAT GGATATTTCT AAACAGTTAC ATGCCTATGA GGTGGAGTAT
    CACGTGCTAC AGGATGAACT CCAAGAATCT TCCTATGCTT GCGAGGACAG TGAGCCCATA
    GAGAAGCTGG AGAGGGCCAA CAGCCAACTG AAAAGACAAA ACATGGACCT TCTAGAAAAA
    TTACAGATAG CTCATGCTAA AATTCAGACT TTGGAGTCCA ACCTGGAAAA TCTTTTAACC
    AGAGAGACCA AAATGAAGTC TTTGATCCGG ACCCTGGAAC AGGAGAAGAC GGCTTATCAG
    AAGGCAGTGG AGCAGATCTG GAAGCTGCTG CCTGCAGATA TTTTAGCCAA CTGCGAACCA
    CTGCTGAGAG ACTTAAACTG CAACCCTAAC AACAAAGCCA AGACAGGAAA CAAGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010809148.1
    CDS307..4086
    Translation

    Target ORF information:

    RefSeq Version XM_010810846.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 4 (TBC1D4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810846.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGCCCC CCAGCTGCAT TCAGGATGAG CCGTTCCCGC ACCCACTGGA GCCCGAGCCT 
    GGCACCCCGG CGCAGCCTGG CCCGGGGAAG CCGAGCGACA AAAGGTTCCG GCTGTGGTAC
    GTAGGGGGGT CGTGCCTGGA CCGCAGGACC ACGCTACCCA TGCTGCCCTG GCTCATGGCC
    GAGATCCGCC GGCGCAGCCA GAAGCCCGAG GCGGGCGGCT GCGGGGCGCC GGCGGCCCGC
    GAGGTGCTTC TGGTGCTCAG CGCGCCCTTC CTGCGTTGCA TCCCTGCGCC GGGCGCCGGG
    GCCACTGGGG GCTCCGGCTC GACGGCCGCG CAGCCCAACC CGGCCGTGTT CATTTTCGAG
    CACAAGGCGC AACACATCTC GCGCTTCATC CACAACAGCC AGGACCTCAC CTACTTTGCT
    TACCTGATCA AGGCTCAGCC CGACGACCCG GAGTCACAGA TGGCCTGCCA TGTTTTCCGC
    GCTGCAGACC CCAATCAGGT TCCTGATGTT ATCAGCAGCA TAAGGCAGTT ATCGAAAGCT
    GCCATGAAAG AGGATGCAAA ACCCAGCAAA GACAACGAGG ACGCCTTTTA CAACTCTCAG
    AAGTTCGAGG TCCTGTACTG TGGCAGGGTG ACCGTGACCC ACAAGAAGGC GCCGTCCAGC
    CTCATCGACG ACTGCATCGA GAAGTTCAGC CTGCACGAGC AGCAGCGCTT GCGGCTCCAG
    AGCGAGCCGC GCGCAGGGGA CTCGGGCGAA GACCAGGCGG GATCCGAGGC CCGAACGCCG
    GCCTGCCCCG TCGACGTTCT GTCGGAGGAA GGGGATGGCG TCGCCGACAC CCACCCGGTC
    TTCCCTGCCG CGCCCAGCCA GCCTGGGCCA TCTAGCTCGC GAGTCTGCTT CCCTGAGAGG
    ATTTTGGAAG ATTCGGGCTT CGATGAACAG CAGGAGTTCC GGTCTCGGTG CAGCAGCGTC
    ACGGGGGTCC TGCAAAGGAA AGTTCACGAG AACAGCCAGA AGCCGCAGCC GCGGCGGAGG
    CATGCCAGCG CGCCCAGCCA CGTGCAGCCC TCGGACTCGG AGAAGAACAG AACCATGCTG
    TTTCAGGTTG GGCGGTTTGA GATTAACCTT ATCAGTCCGG ACACTAAATC AGTTGTGCTT
    GAGAAGAATT TTAAAGATAT CTCGTCTTGT TCCCAGGGTA TAAAGCATGT TGATCACTTC
    GGCTTTATTT GTCGGGAGTC CTCGGAGCCT GGACTGAGCC AGTATATCTG CTACGTGTTC
    CAGTGTGCCA GTGAATCTCT GGTTGATGAG GTAATGCTGA CTCTGAAACA GGCTTTCAGC
    ACGGCCGCTG CCCTGCAGAG TGCCAAGACC CAGATTAAGC TGTGTGAGGC CTGCCCCATG
    CACTCTTTGC ATAAGCTCTG TGAAAGGATC GAAGGTCTCT ACCCACCAAG AGCCAAGCTG
    GTAATACAGA GGCATCTCTC ATCCCTGACA GATAACGAGC AAGCTGACAT CTTTGAACGC
    GTTCAGAAAA TGAAGGCAGT CAATGACCAG GAGGAGAATG AACTTGTGAT TTTACATCTG
    AGGCAGCTAT GCGAAGCCAA GCAGAAGACA CATGTTCATA TCGGGGAAGG CCCATTGGGA
    GCTAACAGAA TGAGAGGTCG GCTTGGAAGT GTGGACAGCT TTGAACGATC CAACAGTGTT
    GCTTCAGAGA AGGACTACTC GCCAGGAGAC TCTCCGCCAG GGACCCCGCC AGCTTCCCCC
    CTGTCCTCAG CTTGGCACAC ATTCCCTGAG GAGGATTCCG ACTCCCCGCA GTTTCGAAGA
    CGGGCCCACA CATTCAGCCA CCCACCTTCA AGCACGAAGA GGAAGCTGAA TTTGCAGGAT
    GGGAAGGCCC ATGGGGTTCG CTCCCCTCTT CTGAGGCAGA GCTCCAGTGA GCAGTGCAGC
    ATCCTTCCAC CAGCTCGGCG CATGCACAAG GAGTGTAATT CTTCCTCCAG TCTTCCAAGT
    CTTCACACTT CCTTCTCTGC CCCTTCCTTC ACTGCCCCCC CTTTCCTGAA AAGCTTTTAC
    CAGAATTCAG GTAGACTATC CCCACAGTAT GAGAATGAAA TCAGACAAGA CACTGCTTCA
    GAATCAAGTG ATGGAGAGGG GAGAAAAAGG ACCTCGTCCA CCTGCAGCAA TGAATCCCTA
    AACGCTGGAG GAACCCCCGT CACTCCTCGC CGAGTTTCCT GGCGGCAGCG CATCTTCCTC
    AGGGTTGCTT CGCCCATGAG GAAATCTCCC TCAGCGATGC AGCAGGATGG CCTGGACAGG
    AGTGAGCTGC TGCCACTGTC CCCTCTTGCT CCAACCATGG AGGAGGAACC TCTAATGATA
    TTCATGTCTG ATGAAGATGA CCCAGAAAAG GGTGAAGAGA GAAACAAGTC GGAGGAACTG
    AGGTGTTTGT GGAGAAAAGC TATACACCAG CAGATCCTGT TGCTTCGAAT GGAAAAAGAA
    AACCAGAAAC TTGAAGCAAG CAGAGATGAA CTGCAGTCCA GAAAAGTTAA ATTGGATTAT
    GAAGAAGTTG GTGCATGTCA GAAGGAGGTC TTAACAATCT GGGATAAGAA GTTGTTAAAC
    TGCAGAGCTA AAATCAGATG CGATATGGAA GATATTCATA CTTCTCTTAA AGAAGGTGTT
    CCCAAAAGTC GACGAGGAGA AATCTGGCAG TTCCTAGCTT TGCAATATCG TCTGAGACAC
    AGATTGCCTA ATAAACAACA ACCTCCCGAC ATATCCTATA AAGAGCTTTT AAAACAGCTC
    ACGGCTCAGC AGCATGCTAT CCTCGTGGAT TTAGGGAGAA CATTTCCTAC TCATCCTTAC
    TTTTCAGCAC AGCTTGGGGC AGGGCAGCTG TCGCTCTTTA ATCTCTTGAA AGCCTACTCA
    TTACTGGACA AAGAAGTGGG TTACTGTCAG GGGATCAGCT TTGTGGCTGG AGTCTTGCTT
    CTACACATGA GTGAAGAGCA AGCCTTTGAA ATGCTGAAAT TCCTCATGTA TGACCTGGGC
    TTCCGCAAGC AGTACCGACC TGACATGATG TCGCTGCAGA TTCAAATGTA CCAGCTGTCC
    AGGCTCCTTC ATGATTATCA CAGAGATCTC TACAATCATC TTGAAGAAAA TGAAATCAGC
    CCCAGTCTTT ATGCTGCCCC CTGGTTCCTC ACGTTGTTTG CCTCTCAATT TCCATTAGGA
    TTTGTAGCCA GAGTGTTTGA TATTATTTTT CTTCAGGGAA CTGAAGTTAT ATTTAAGGTT
    GCCCTAAGCC TGTTGAGCAA CCAAGAGACA CTCATAATGG AATGTGAAAA CTTTGAAAAT
    ATTGTTGAAT TCCTTAAAAT CACTCTCCCC GATATGAATT CATCTGAAAT GGAAAAAATA
    ATCACCCAGG TTTTTGAGAT GGATATTTCT AAACAGTTAC ATGCCTATGA GGTGGAGTAT
    CACGTGCTAC AGGATGAACT CCAAGAATCT TCCTATGCTT GCGAGGACAG TGAGCCCATA
    GAGAAGCTGG AGAGGGCCAA CAGCCAACTG AAAAGACAAA ACATGGACCT TCTAGAAAAA
    TTACAGATAG CTCATGCTAA AATTCAGACT TTGGAGTCCA ACCTGGAAAA TCTTTTAACC
    AGAGAGACCA AAATGAAGTC TTTGATCCGG ACCCTGGAAC AGGAGAAGAC GGCTTATCAG
    AAGGCAGTGG AGCAGATCTG GAAGCTGCTG CCTGCAGATA TTTTAGCCAA CTGCGAACCA
    CTGCTGAGAG ACTTAAACTG CAACCCTAAC AACAAAGCCA AGACAGGAAA CAAGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.