EIF4G3 cDNA ORF clone, Bos taurus(cattle)

The following EIF4G3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF4G3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa88097 XM_015460889.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88097 XM_015462483.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa227919 XM_024983016.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa227920 XM_024983022.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa227921 XM_024983027.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa88089 XM_015462472.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa88089 XM_015460870.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa88090 XM_015462473.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa88090 XM_015460871.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa88091 XM_015462474.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88091 XM_015460872.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88092 XM_015462475.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88092 XM_015460874.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88093 XM_015460876.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88093 XM_015462476.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88094 XM_015460878.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88094 XM_015462477.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88095 XM_015462480.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88095 XM_015460884.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88096 XM_024983008.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88096 XM_024983012.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88096 XM_015462482.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88096 XM_015460886.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa03120 XM_015460883.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa03120 XM_015462479.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa03120 XM_024982991.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa03122 XM_010802672.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa03122 XM_024983019.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa03122 XM_010826706.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa03122 XM_024982942.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa03122 XM_010802671.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa03122 XM_015460885.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa03122 XM_024982993.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa03122 XM_010826709.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa03122 XM_015462481.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa52861 XM_024982977.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa52861 XM_015460880.2
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa52861 XM_015462478.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa52861 XM_024982968.1
Latest version!
Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OBa88092 XM_015460874.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88090 XM_015460871.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa88089 XM_015460870.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa88094 XM_015460878.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa52861 XM_015460880.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa88095 XM_015460884.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88097 XM_015460889.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa03120 XM_015460883.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa88096 XM_015460886.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88091 XM_015460872.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa88093 XM_015460876.1 Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa88097
    Clone ID Related Accession (Same CDS sequence) XM_015462483.1 , XM_015460889.1 , XM_015460889.2
    Accession Version XM_015462483.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2778bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGGTAAGAA GAGAGGAAGG AGTAAAACCA
    GAAATCTCCA AGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317969.1
    CDS162..2939
    Translation

    Target ORF information:

    RefSeq Version XM_015462483.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462483.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGGTAAGAA GAGAGGAAGG AGTAAAACCA
    GAAATCTCCA AGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88097
    Clone ID Related Accession (Same CDS sequence) XM_015462483.1 , XM_015460889.1 , XM_015460889.2
    Accession Version XM_015460889.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2778bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGGTAAGAA GAGAGGAAGG AGTAAAACCA
    GAAATCTCCA AGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316375.1
    CDS162..2939
    Translation

    Target ORF information:

    RefSeq Version XM_015460889.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460889.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGGTAAGAA GAGAGGAAGG AGTAAAACCA
    GAAATCTCCA AGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88097
    Clone ID Related Accession (Same CDS sequence) XM_015462483.1 , XM_015460889.1 , XM_015460889.2
    Accession Version XM_015460889.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2778bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGGTAAGAA GAGAGGAAGG AGTAAAACCA
    GAAATCTCCA AGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316375.1
    CDS196..2973
    Translation

    Target ORF information:

    RefSeq Version XM_015460889.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460889.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGGTAAGAA GAGAGGAAGG AGTAAAACCA
    GAAATCTCCA AGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227919
    Clone ID Related Accession (Same CDS sequence) XM_024983016.1
    Accession Version XM_024983016.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTCTGTTGA ATGTTGGAGC ACGGAGATCT
    CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG ATCATCACGG TCTCCGTGAA AGAAGACGTG
    CACCTGAAGA AGGCAGAGAA CGCCTGGAAG CCCAGCCAGA GGCGGGATAG CCCAGCCGAG
    GACCCCGAGA ACATCAAGAC CCAGGAACTT TTTAGAAAAG TTCGAAGTAT CTTAAATAAA
    TTGACACCAC AGATGTTCAA CCAACTGATG AAGCAAGTGT CAGGACTTAC TGTTGACACA
    GAGGAGCGGC TGAAAGGAGT CATTGACCTG GTCTTTGAGA AGGCTATTGA CGAACCCAGT
    TTCTCTGTGG CTTACGCAAA CATGTGTCGA TGTCTAGTAA CGCTGAAAGT ACCCATGGCA
    GACAAGCCTG GTAACACAGT GAACTTCCGG AAGCTACTAC TGAACCGTTG CCAGAAGGAA
    TTTGAAAAAG ATAAAGCCGA TGATGATGTC TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT
    GCCAGTGCTC CAGAGGAGAG GACAAGGCTT CATGATGAAC TGGAAGAAGC TAAAGATAAA
    GCCCGGCGGA GATCCATTGG CAACATCAAG TTCATTGGAG AACTCTTTAA ACTCAAAATG
    CTGACTGAAG CCATCATGCA TGACTGTGTG GTGAAGCTGC TGAAGAACCA CGATGAAGAA
    TCCCTCGAGT GTCTGTGTCG CCTGCTCACC ACAATTGGCA AAGACCTGGA CTTTGAGAAA
    GCAAAGCCAC GTATGGACCA GTATTTTAAT CAGATGGAGA AAATTGTAAA AGAAAGAAAA
    ACCTCATCTA GGATTCGATT CATGCTTCAG GACGTAATAG ATCTAAGGCT GTGTAATTGG
    GTATCACGAC GAGCAGATCA GGGGCCTAAA ACTATTGAAC AGATTCACAA AGAAGCTAAA
    ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC CAACAACTCA TGACCAAAGA GAAGAGAAGA
    CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG TGGAACACTG TTCAAGGGGC CAAGAATAGT
    CGGGTATTGG ACCCCTCAAA ATTTCTGAAA ATCACTAAGC CCACAATTGA TGAGAAAATT
    CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG
    GCGAGCGAGG CCGCACCTTC AGGGTCTGCG TCATCCACAC CTCTAGAGTT TGATTCCCGG
    CGGACCTTAA CTAGTCGAGG AAGCACGGGC AGGGAGAAGA ATGACAAGCC CCTCCAGCCT
    GCAGCAGCTC GGCCGAACAC TTTCATGAGG GGTGGCAGCA GTAAAGACCT GTTAGACAAT
    CAGTCTCAGG AGGAGCAGCG GAGGGAGATG CTGGAGACCG TGAAGCAGCT CACAGGAGGC
    ATGGACGTGG AGAGGAGCAG CGCTGAGGCG GAGCGAAGCA GGACCAGGGA GCCAGTGACA
    AAACCGGAAA TTCCAGTAAC ATCAGCCTCT GACAAGCCCG CGTTGTCAGA GGAAGAGATG
    GAGAGGAAGT CCAAATCTAT CATTGATGAG TTTCTACACA TTAATGATTT TAAGGAAGCC
    ATGCAGTGTG TGGAAGAGCT GAACGCCCAG GGCCTGCAAC ATGTGTTCGT GAGGACGGGA
    GTGGAGTCCA CCCTGGAAAG GAGCCAGATC ACCCGGGATC ACATGGGCCA GTTACTGTAT
    CAGCTGGTGC AGTCAGAAAA GCTCAGCAAA CAGGACTTTT TCAGAGGTTT TTCAGAAACA
    TTGGAATTGG CAGATGACAT GGCCATTGAT ATTCCCCATA TTTGGTTGTA CCTTGCTGAA
    CTGGTGACTC CCATGTTAAA AGAAGGCGGA ATCTCCATGA GACAACTCAT CACAGAATTT
    TGCAAACCTT TACTTCCTGT TGGAAGAGCT GGAATCTTGA TTTCTGAAAT ATTGCACCTT
    CTATGCAAAC AAATGAGCCA TAAGAAAGTG GGAGCCCTAT GGAGGGAGGC TGACCTCAGC
    TGGAAGGACT TTTTACCAGA AGGGGAAGAT GTACATAATT TTCTCTTGGA GCAGAAGTTG
    GACTTTTTAG AGTCTGACAG TTCCTGTTCC TCCGAAGCAC TTTTGAAGAA AGAACTCTCT
    GCCGAAGAGC TGTATAAGCG ACTCGAGAAA CTCATTATTG AGGACAAGGC AAATGATGAA
    CAGATCTTTG ACTGGGTAGA GGCTAATCTA GATGAAAGCC AGATGAGTTC ACCTACATTC
    CTTAGAGCTT TAATGACAGC CGTCTGTAAA GCAGCTATTA TAGCCGACTG TTCGACCTTC
    CGAGTGGACA CTGCTGTTAT CAAGCAGAGA GTGCCGATCT TACTCAAGTA CCTAGACTCA
    GATACAGAGA AGGAACTGCA AGCACTTTAT GCACTACAAG CATCAATAGT AAAACTTGAT
    CAACCTGCCA ATTTGCTACG GATGTTTTTT GATTGCCTGT ATGACGAGGA GGTGATCTCT
    GAGGATGCCT TCTACAAGTG GGAGAGCAGC AAGGACCCGG CAGAGCAGAA TGGGAAGGGT
    GTGGCCCTGA AGTCTGTCAC GGCGTTCTTC ACATGGCTGC GGGAAGCAGA AGAGGAGTCT
    GAGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838784.1
    CDS196..5067
    Translation

    Target ORF information:

    RefSeq Version XM_024983016.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983016.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTCTGTTGA ATGTTGGAGC ACGGAGATCT
    CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG ATCATCACGG TCTCCGTGAA AGAAGACGTG
    CACCTGAAGA AGGCAGAGAA CGCCTGGAAG CCCAGCCAGA GGCGGGATAG CCCAGCCGAG
    GACCCCGAGA ACATCAAGAC CCAGGAACTT TTTAGAAAAG TTCGAAGTAT CTTAAATAAA
    TTGACACCAC AGATGTTCAA CCAACTGATG AAGCAAGTGT CAGGACTTAC TGTTGACACA
    GAGGAGCGGC TGAAAGGAGT CATTGACCTG GTCTTTGAGA AGGCTATTGA CGAACCCAGT
    TTCTCTGTGG CTTACGCAAA CATGTGTCGA TGTCTAGTAA CGCTGAAAGT ACCCATGGCA
    GACAAGCCTG GTAACACAGT GAACTTCCGG AAGCTACTAC TGAACCGTTG CCAGAAGGAA
    TTTGAAAAAG ATAAAGCCGA TGATGATGTC TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT
    GCCAGTGCTC CAGAGGAGAG GACAAGGCTT CATGATGAAC TGGAAGAAGC TAAAGATAAA
    GCCCGGCGGA GATCCATTGG CAACATCAAG TTCATTGGAG AACTCTTTAA ACTCAAAATG
    CTGACTGAAG CCATCATGCA TGACTGTGTG GTGAAGCTGC TGAAGAACCA CGATGAAGAA
    TCCCTCGAGT GTCTGTGTCG CCTGCTCACC ACAATTGGCA AAGACCTGGA CTTTGAGAAA
    GCAAAGCCAC GTATGGACCA GTATTTTAAT CAGATGGAGA AAATTGTAAA AGAAAGAAAA
    ACCTCATCTA GGATTCGATT CATGCTTCAG GACGTAATAG ATCTAAGGCT GTGTAATTGG
    GTATCACGAC GAGCAGATCA GGGGCCTAAA ACTATTGAAC AGATTCACAA AGAAGCTAAA
    ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC CAACAACTCA TGACCAAAGA GAAGAGAAGA
    CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG TGGAACACTG TTCAAGGGGC CAAGAATAGT
    CGGGTATTGG ACCCCTCAAA ATTTCTGAAA ATCACTAAGC CCACAATTGA TGAGAAAATT
    CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG
    GCGAGCGAGG CCGCACCTTC AGGGTCTGCG TCATCCACAC CTCTAGAGTT TGATTCCCGG
    CGGACCTTAA CTAGTCGAGG AAGCACGGGC AGGGAGAAGA ATGACAAGCC CCTCCAGCCT
    GCAGCAGCTC GGCCGAACAC TTTCATGAGG GGTGGCAGCA GTAAAGACCT GTTAGACAAT
    CAGTCTCAGG AGGAGCAGCG GAGGGAGATG CTGGAGACCG TGAAGCAGCT CACAGGAGGC
    ATGGACGTGG AGAGGAGCAG CGCTGAGGCG GAGCGAAGCA GGACCAGGGA GCCAGTGACA
    AAACCGGAAA TTCCAGTAAC ATCAGCCTCT GACAAGCCCG CGTTGTCAGA GGAAGAGATG
    GAGAGGAAGT CCAAATCTAT CATTGATGAG TTTCTACACA TTAATGATTT TAAGGAAGCC
    ATGCAGTGTG TGGAAGAGCT GAACGCCCAG GGCCTGCAAC ATGTGTTCGT GAGGACGGGA
    GTGGAGTCCA CCCTGGAAAG GAGCCAGATC ACCCGGGATC ACATGGGCCA GTTACTGTAT
    CAGCTGGTGC AGTCAGAAAA GCTCAGCAAA CAGGACTTTT TCAGAGGTTT TTCAGAAACA
    TTGGAATTGG CAGATGACAT GGCCATTGAT ATTCCCCATA TTTGGTTGTA CCTTGCTGAA
    CTGGTGACTC CCATGTTAAA AGAAGGCGGA ATCTCCATGA GACAACTCAT CACAGAATTT
    TGCAAACCTT TACTTCCTGT TGGAAGAGCT GGAATCTTGA TTTCTGAAAT ATTGCACCTT
    CTATGCAAAC AAATGAGCCA TAAGAAAGTG GGAGCCCTAT GGAGGGAGGC TGACCTCAGC
    TGGAAGGACT TTTTACCAGA AGGGGAAGAT GTACATAATT TTCTCTTGGA GCAGAAGTTG
    GACTTTTTAG AGTCTGACAG TTCCTGTTCC TCCGAAGCAC TTTTGAAGAA AGAACTCTCT
    GCCGAAGAGC TGTATAAGCG ACTCGAGAAA CTCATTATTG AGGACAAGGC AAATGATGAA
    CAGATCTTTG ACTGGGTAGA GGCTAATCTA GATGAAAGCC AGATGAGTTC ACCTACATTC
    CTTAGAGCTT TAATGACAGC CGTCTGTAAA GCAGCTATTA TAGCCGACTG TTCGACCTTC
    CGAGTGGACA CTGCTGTTAT CAAGCAGAGA GTGCCGATCT TACTCAAGTA CCTAGACTCA
    GATACAGAGA AGGAACTGCA AGCACTTTAT GCACTACAAG CATCAATAGT AAAACTTGAT
    CAACCTGCCA ATTTGCTACG GATGTTTTTT GATTGCCTGT ATGACGAGGA GGTGATCTCT
    GAGGATGCCT TCTACAAGTG GGAGAGCAGC AAGGACCCGG CAGAGCAGAA TGGGAAGGGT
    GTGGCCCTGA AGTCTGTCAC GGCGTTCTTC ACATGGCTGC GGGAAGCAGA AGAGGAGTCT
    GAGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227920
    Clone ID Related Accession (Same CDS sequence) XM_024983022.1
    Accession Version XM_024983022.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTTTCCAGA GGCCTCAAAT ACAGCCTCCC 
    AGAGCCACCA TCCCGAACAG CAGCCCTTCC ATTCGACCCG GTGCACAGAC ACCCACTGCA
    GTGTATCAGG CCAATCAGCA CATCATGATG GTTAACCATC TGCCCATGCC GTACCCAGTG
    CCCCAGGGTC CTCAGTACTG TATACCACAG TACCGTCACA GTGGCCCTCC GTATGTTGGG
    CCACCACAAC AGTACCCGGT TCAACCACCA GGACCAGGTC CTTTTTATCC TGGGCCAGGA
    CCTGGGGACT TCCCCAGTGC TTACGGAACG CCTTTTTACC CAAGTCAGCC AGTGTACCAG
    TCAGCACCTA TCATAGTGCC TACGCAGCAG CAGCCCCCTC CAGCCAAGAG AGAGAAAAAA
    ACAATAAGAA TTCGGGATCC AAACCAGGGA GGTAAAGACA TAACAGAGGA GATTATGTCT
    GGAGGTGGCA GCCGAAATCC TACTCCACCC ATAGGGAGAC CCACTTCCAC ACCTACTCCT
    CCTCAGCTGC CCAGCCAGGT CCCCGAGCAC AGCCCTGTGG TCTATGGGAC TGTGGAGAGC
    GCTCATCTTG CTGCCAGCAC CCCTGTCACT GCAGCGAGCG ACCAGAAGCA AGAAGAGAAG
    CCAAAACCAG ATCCAGTGTT AAAGACTCCT TCCCCAGTCC TTAGGCTAGC CCTCAGTGGA
    GAGAAGAAAG AACAAGCAGG CCCGACGTCT GAGATTACTG CAGTAGAGTC CATACCAGAG
    CTTCCTCTGC CTCCATCCCC TACTGCTATT TCTCCACTTG CTCGAAGTAC AATTACTTCT
    CCCACCTCTG TTGCTCTTAG TAGCCAACCA ATATTCACCA CTGCAATTGA TGACAGATGT
    GAACTCTCTT CCTCAAAAGA AGATACAATT CCTATTCCCA GCCCCACATC TTGCACAGAA
    GCATCAGATC CTTTACCAAC AGATGAAATT GATGATGATA TATGCAAGAA ATCTTGTAGT
    GTAGCACCAA ATGATATTCC ACTGATTTCT AGTACTAACC TAATTAATGA AATGAATGGA
    GTTAATGAAA AATTACCAGC CACGGAAAGC ATTGTGGAAA TAGTAAAACA GGAGGTGTTA
    CCGCTGACTC TTGAATTGGA GATTTTGGAA AATCCCCCTG AAGAAATCAA AGTGGAGTGT
    GTCCCAACTC CCATCACCCC TTCGATAGTT CCCTCCTCTT CTCCGTCTCC TCCAGCTCCT
    CCAGCTTCTC CTTCTCCCAC ACCAGTTATC GTTCCTGCTG CTGCCACCAG TGTTAATACT
    GGTCGTGGTT CCACCGGAGT CCAGAGAGCC TTCGAAGAGG GCGAGAACAT AAGAACTTGC
    CTTAGTGAAG ATGCAAAAGA GATTCAGAAC AAAGTAGAGG TAGAAGCAGA TGGGCAAACA
    GAAGAGATTG TGGATTCTCA GAACTTAAGT TCAAGAAAGA GCCCAGTCCC AGCTCAGACA
    GCTATACCTG CACCAAAGAT GTGGAAGAAA CCAAAAGACC GGACCCGAGC CACTGAAGAG
    TCGTTAGAGG CAGAATCAGA GCCTAAAGCT GAAGAGGAGC TTTCAGGTGA CAAAATACTT
    GAATCTGAAC AGGATAAAAT GAGCCAAGGG TTTCATCCTG AAAGAGACCC CTCTGACCTA
    AAAAACGTGA AAACTGTGGA AGAAAATGGA GAAGAAGCTG AGCCTGTACG TAATGGTGCT
    GAGAGTATTT CTGAGGGTGA AGGAATAGAT GCTAATTCAG GCTCCACTGA TAGTTCTGGT
    GAAGGGGTCA CATTCCCATT TAAGTCAGAA TCCTGGAAGC CTCCTGATTC GGAAGGCAAG
    AAGCAGTATG ACAGGGAGTT TTTGCTGGAT TTCCAATTCA TGCCAGCGTG TATTCAGAAA
    CCAGAGGGCC TGCCTCCCAT CAGTGATGTG GTTCTTGACA AGATCAATCA ACCAAAATTG
    CCAATGCGAA CTCTGGATCC TCGGATTTTG CCTCGAGGAC CAGACTTTAC TCCAGCCTTT
    GCAGATTTTG GAAGGCAAGC CCCTGGTGGA AGAGGTGTAC CTGTTTGCAA AGTGCAGAGC
    AGGCATGGAT TGCCAATTCT GGAACAGAGC AAAGCCCCAG CTTCCACTCC ACTGGCGATG
    TCGCACCCAC CCCTGAAGAG CCTGCCTCTA GGGCTGTTGA ATGTTGGAGC ACGGAGATCT
    CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG ATCATCACGG TCTCCGTGAA AGAAGACGTG
    CACCTGAAGA AGGCAGAGAA CGCCTGGAAG CCCAGCCAGA GGCGGGATAG CCCAGCCGAG
    GACCCCGAGA ACATCAAGAC CCAGGAACTT TTTAGAAAAG TTCGAAGTAT CTTAAATAAA
    TTGACACCAC AGATGTTCAA CCAACTGATG AAGCAAGTGT CAGGACTTAC TGTTGACACA
    GAGGAGCGGC TGAAAGGAGT CATTGACCTG GTCTTTGAGA AGGCTATTGA CGAACCCAGT
    TTCTCTGTGG CTTACGCAAA CATGTGTCGA TGTCTAGTAA CGCTGAAAGT ACCCATGGCA
    GACAAGCCTG GTAACACAGT GAACTTCCGG AAGCTACTAC TGAACCGTTG CCAGAAGGAA
    TTTGAAAAAG ATAAAGCCGA TGATGATGTC TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT
    GCCAGTGCTC CAGAGGAGAG GACAAGGCTT CATGATGAAC TGGAAGAAGC TAAAGATAAA
    GCCCGGCGGA GATCCATTGG CAACATCAAG TTCATTGGAG AACTCTTTAA ACTCAAAATG
    CTGACTGAAG CCATCATGCA TGACTGTGTG GTGAAGCTGC TGAAGAACCA CGATGAAGAA
    TCCCTCGAGT GTCTGTGTCG CCTGCTCACC ACAATTGGCA AAGACCTGGA CTTTGAGAAA
    GCAAAGCCAC GTATGGACCA GTATTTTAAT CAGATGGAGA AAATTGTAAA AGAAAGAAAA
    ACCTCATCTA GGATTCGATT CATGCTTCAG GACGTAATAG ATCTAAGGCT GTGTAATTGG
    GTATCACGAC GAGCAGATCA GGGGCCTAAA ACTATTGAAC AGATTCACAA AGAAGCTAAA
    ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC CAACAACTCA TGACCAAAGA GAAGAGAAGA
    CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG TGGAACACTG TTCAAGGGGC CAAGAATAGT
    CGGGTATTGG ACCCCTCAAA ATTTCTGAAA ATCACTAAGC CCACAATTGA TGAGAAAATT
    CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG
    GCGAGCGAGG CCGATGCCTT AAGATCAAGT GCTTCCAGTT TAAACAGATT CTCTGCTTTG
    CAACCTCCAG CACCTTCAGG GTCTGCGTCA TCCACACCTC TAGAGTTTGA TTCCCGGCGG
    ACCTTAACTA GTCGAGGAAG CACGGGCAGG GAGAAGAATG ACAAGCCCCT CCAGCCTGCA
    GCAGCTCGGC CGAACACTTT CATGAGGGGT GGCAGCAGTA AAGACCTGTT AGACAATCAG
    TCTCAGGAGG AGCAGCGGAG GGAGATGCTG GAGACCGTGA AGCAGCTCAC AGGAGGCATG
    GACGTGGAGA GGAGCAGCGC TGAGGCGGAG CGAAGCAGGA CCAGGGAGCC AGTGACAAAA
    CCGGAAATTC CAGTAACATC AGCCTCTGAC AAGCCCGCGT TGTCAGAGGA AGAGATGGAG
    AGGAAGTCCA AATCTATCAT TGATGAGTTT CTACACATTA ATGATTTTAA GGAAGCCATG
    CAGTGTGTGG AAGAGCTGAA CGCCCAGGGC CTGCAACATG TGTTCGTGAG GACGGGAGTG
    GAGTCCACCC TGGAAAGGAG CCAGATCACC CGGGATCACA TGGGCCAGTT ACTGTATCAG
    CTGGTGCAGT CAGAAAAGCT CAGCAAACAG GACTTTTTCA GAGGTTTTTC AGAAACATTG
    GAATTGGCAG ATGACATGGC CATTGATATT CCCCATATTT GGTTGTACCT TGCTGAACTG
    GTGACTCCCA TGTTAAAAGA AGGCGGAATC TCCATGAGAC AACTCATCAC AGAATTTTGC
    AAACCTTTAC TTCCTGTTGG AAGAGCTGGA ATCTTGATTT CTGAAATATT GCACCTTCTA
    TGCAAACAAA TGAGCCATAA GAAAGTGGGA GCCCTATGGA GGGAGGCTGA CCTCAGCTGG
    AAGGACTTTT TACCAGAAGG GGAAGATGTA CATAATTTTC TCTTGGAGCA GAAGTTGGAC
    TTTTTAGAGT CTGACAGTTC CTGTTCCTCC GAAGCACTTT TGAAGAAAGA ACTCTCTGCC
    GAAGAGCTGT ATAAGCGACT CGAGAAACTC ATTATTGAGG ACAAGGCAAA TGATGAACAG
    ATCTTTGACT GGGTAGAGGC TAATCTAGAT GAAAGCCAGA TGAGTTCACC TACATTCCTT
    AGAGCTTTAA TGACAGCCGT CTGTAAAGCA GCTATTATAG CCGACTGTTC GACCTTCCGA
    GTGGACACTG CTGTTATCAA GCAGAGAGTG CCGATCTTAC TCAAGTACCT AGACTCAGAT
    ACAGAGAAGG AACTGCAAGC ACTTTATGCA CTACAAGCAT CAATAGTAAA ACTTGATCAA
    CCTGCCAATT TGCTACGGAT GTTTTTTGAT TGCCTGTATG ACGAGGAGGT GATCTCTGAG
    GATGCCTTCT ACAAGTGGGA GAGCAGCAAG GACCCGGCAG AGCAGAATGG GAAGGGTGTG
    GCCCTGAAGT CTGTCACGGC GTTCTTCACA TGGCTGCGGG AAGCAGAAGA GGAGTCTGAG
    GATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838790.1
    CDS465..5333
    Translation

    Target ORF information:

    RefSeq Version XM_024983022.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983022.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTTTCCAGA GGCCTCAAAT ACAGCCTCCC 
    AGAGCCACCA TCCCGAACAG CAGCCCTTCC ATTCGACCCG GTGCACAGAC ACCCACTGCA
    GTGTATCAGG CCAATCAGCA CATCATGATG GTTAACCATC TGCCCATGCC GTACCCAGTG
    CCCCAGGGTC CTCAGTACTG TATACCACAG TACCGTCACA GTGGCCCTCC GTATGTTGGG
    CCACCACAAC AGTACCCGGT TCAACCACCA GGACCAGGTC CTTTTTATCC TGGGCCAGGA
    CCTGGGGACT TCCCCAGTGC TTACGGAACG CCTTTTTACC CAAGTCAGCC AGTGTACCAG
    TCAGCACCTA TCATAGTGCC TACGCAGCAG CAGCCCCCTC CAGCCAAGAG AGAGAAAAAA
    ACAATAAGAA TTCGGGATCC AAACCAGGGA GGTAAAGACA TAACAGAGGA GATTATGTCT
    GGAGGTGGCA GCCGAAATCC TACTCCACCC ATAGGGAGAC CCACTTCCAC ACCTACTCCT
    CCTCAGCTGC CCAGCCAGGT CCCCGAGCAC AGCCCTGTGG TCTATGGGAC TGTGGAGAGC
    GCTCATCTTG CTGCCAGCAC CCCTGTCACT GCAGCGAGCG ACCAGAAGCA AGAAGAGAAG
    CCAAAACCAG ATCCAGTGTT AAAGACTCCT TCCCCAGTCC TTAGGCTAGC CCTCAGTGGA
    GAGAAGAAAG AACAAGCAGG CCCGACGTCT GAGATTACTG CAGTAGAGTC CATACCAGAG
    CTTCCTCTGC CTCCATCCCC TACTGCTATT TCTCCACTTG CTCGAAGTAC AATTACTTCT
    CCCACCTCTG TTGCTCTTAG TAGCCAACCA ATATTCACCA CTGCAATTGA TGACAGATGT
    GAACTCTCTT CCTCAAAAGA AGATACAATT CCTATTCCCA GCCCCACATC TTGCACAGAA
    GCATCAGATC CTTTACCAAC AGATGAAATT GATGATGATA TATGCAAGAA ATCTTGTAGT
    GTAGCACCAA ATGATATTCC ACTGATTTCT AGTACTAACC TAATTAATGA AATGAATGGA
    GTTAATGAAA AATTACCAGC CACGGAAAGC ATTGTGGAAA TAGTAAAACA GGAGGTGTTA
    CCGCTGACTC TTGAATTGGA GATTTTGGAA AATCCCCCTG AAGAAATCAA AGTGGAGTGT
    GTCCCAACTC CCATCACCCC TTCGATAGTT CCCTCCTCTT CTCCGTCTCC TCCAGCTCCT
    CCAGCTTCTC CTTCTCCCAC ACCAGTTATC GTTCCTGCTG CTGCCACCAG TGTTAATACT
    GGTCGTGGTT CCACCGGAGT CCAGAGAGCC TTCGAAGAGG GCGAGAACAT AAGAACTTGC
    CTTAGTGAAG ATGCAAAAGA GATTCAGAAC AAAGTAGAGG TAGAAGCAGA TGGGCAAACA
    GAAGAGATTG TGGATTCTCA GAACTTAAGT TCAAGAAAGA GCCCAGTCCC AGCTCAGACA
    GCTATACCTG CACCAAAGAT GTGGAAGAAA CCAAAAGACC GGACCCGAGC CACTGAAGAG
    TCGTTAGAGG CAGAATCAGA GCCTAAAGCT GAAGAGGAGC TTTCAGGTGA CAAAATACTT
    GAATCTGAAC AGGATAAAAT GAGCCAAGGG TTTCATCCTG AAAGAGACCC CTCTGACCTA
    AAAAACGTGA AAACTGTGGA AGAAAATGGA GAAGAAGCTG AGCCTGTACG TAATGGTGCT
    GAGAGTATTT CTGAGGGTGA AGGAATAGAT GCTAATTCAG GCTCCACTGA TAGTTCTGGT
    GAAGGGGTCA CATTCCCATT TAAGTCAGAA TCCTGGAAGC CTCCTGATTC GGAAGGCAAG
    AAGCAGTATG ACAGGGAGTT TTTGCTGGAT TTCCAATTCA TGCCAGCGTG TATTCAGAAA
    CCAGAGGGCC TGCCTCCCAT CAGTGATGTG GTTCTTGACA AGATCAATCA ACCAAAATTG
    CCAATGCGAA CTCTGGATCC TCGGATTTTG CCTCGAGGAC CAGACTTTAC TCCAGCCTTT
    GCAGATTTTG GAAGGCAAGC CCCTGGTGGA AGAGGTGTAC CTGTTTGCAA AGTGCAGAGC
    AGGCATGGAT TGCCAATTCT GGAACAGAGC AAAGCCCCAG CTTCCACTCC ACTGGCGATG
    TCGCACCCAC CCCTGAAGAG CCTGCCTCTA GGGCTGTTGA ATGTTGGAGC ACGGAGATCT
    CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG ATCATCACGG TCTCCGTGAA AGAAGACGTG
    CACCTGAAGA AGGCAGAGAA CGCCTGGAAG CCCAGCCAGA GGCGGGATAG CCCAGCCGAG
    GACCCCGAGA ACATCAAGAC CCAGGAACTT TTTAGAAAAG TTCGAAGTAT CTTAAATAAA
    TTGACACCAC AGATGTTCAA CCAACTGATG AAGCAAGTGT CAGGACTTAC TGTTGACACA
    GAGGAGCGGC TGAAAGGAGT CATTGACCTG GTCTTTGAGA AGGCTATTGA CGAACCCAGT
    TTCTCTGTGG CTTACGCAAA CATGTGTCGA TGTCTAGTAA CGCTGAAAGT ACCCATGGCA
    GACAAGCCTG GTAACACAGT GAACTTCCGG AAGCTACTAC TGAACCGTTG CCAGAAGGAA
    TTTGAAAAAG ATAAAGCCGA TGATGATGTC TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT
    GCCAGTGCTC CAGAGGAGAG GACAAGGCTT CATGATGAAC TGGAAGAAGC TAAAGATAAA
    GCCCGGCGGA GATCCATTGG CAACATCAAG TTCATTGGAG AACTCTTTAA ACTCAAAATG
    CTGACTGAAG CCATCATGCA TGACTGTGTG GTGAAGCTGC TGAAGAACCA CGATGAAGAA
    TCCCTCGAGT GTCTGTGTCG CCTGCTCACC ACAATTGGCA AAGACCTGGA CTTTGAGAAA
    GCAAAGCCAC GTATGGACCA GTATTTTAAT CAGATGGAGA AAATTGTAAA AGAAAGAAAA
    ACCTCATCTA GGATTCGATT CATGCTTCAG GACGTAATAG ATCTAAGGCT GTGTAATTGG
    GTATCACGAC GAGCAGATCA GGGGCCTAAA ACTATTGAAC AGATTCACAA AGAAGCTAAA
    ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC CAACAACTCA TGACCAAAGA GAAGAGAAGA
    CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG TGGAACACTG TTCAAGGGGC CAAGAATAGT
    CGGGTATTGG ACCCCTCAAA ATTTCTGAAA ATCACTAAGC CCACAATTGA TGAGAAAATT
    CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG
    GCGAGCGAGG CCGATGCCTT AAGATCAAGT GCTTCCAGTT TAAACAGATT CTCTGCTTTG
    CAACCTCCAG CACCTTCAGG GTCTGCGTCA TCCACACCTC TAGAGTTTGA TTCCCGGCGG
    ACCTTAACTA GTCGAGGAAG CACGGGCAGG GAGAAGAATG ACAAGCCCCT CCAGCCTGCA
    GCAGCTCGGC CGAACACTTT CATGAGGGGT GGCAGCAGTA AAGACCTGTT AGACAATCAG
    TCTCAGGAGG AGCAGCGGAG GGAGATGCTG GAGACCGTGA AGCAGCTCAC AGGAGGCATG
    GACGTGGAGA GGAGCAGCGC TGAGGCGGAG CGAAGCAGGA CCAGGGAGCC AGTGACAAAA
    CCGGAAATTC CAGTAACATC AGCCTCTGAC AAGCCCGCGT TGTCAGAGGA AGAGATGGAG
    AGGAAGTCCA AATCTATCAT TGATGAGTTT CTACACATTA ATGATTTTAA GGAAGCCATG
    CAGTGTGTGG AAGAGCTGAA CGCCCAGGGC CTGCAACATG TGTTCGTGAG GACGGGAGTG
    GAGTCCACCC TGGAAAGGAG CCAGATCACC CGGGATCACA TGGGCCAGTT ACTGTATCAG
    CTGGTGCAGT CAGAAAAGCT CAGCAAACAG GACTTTTTCA GAGGTTTTTC AGAAACATTG
    GAATTGGCAG ATGACATGGC CATTGATATT CCCCATATTT GGTTGTACCT TGCTGAACTG
    GTGACTCCCA TGTTAAAAGA AGGCGGAATC TCCATGAGAC AACTCATCAC AGAATTTTGC
    AAACCTTTAC TTCCTGTTGG AAGAGCTGGA ATCTTGATTT CTGAAATATT GCACCTTCTA
    TGCAAACAAA TGAGCCATAA GAAAGTGGGA GCCCTATGGA GGGAGGCTGA CCTCAGCTGG
    AAGGACTTTT TACCAGAAGG GGAAGATGTA CATAATTTTC TCTTGGAGCA GAAGTTGGAC
    TTTTTAGAGT CTGACAGTTC CTGTTCCTCC GAAGCACTTT TGAAGAAAGA ACTCTCTGCC
    GAAGAGCTGT ATAAGCGACT CGAGAAACTC ATTATTGAGG ACAAGGCAAA TGATGAACAG
    ATCTTTGACT GGGTAGAGGC TAATCTAGAT GAAAGCCAGA TGAGTTCACC TACATTCCTT
    AGAGCTTTAA TGACAGCCGT CTGTAAAGCA GCTATTATAG CCGACTGTTC GACCTTCCGA
    GTGGACACTG CTGTTATCAA GCAGAGAGTG CCGATCTTAC TCAAGTACCT AGACTCAGAT
    ACAGAGAAGG AACTGCAAGC ACTTTATGCA CTACAAGCAT CAATAGTAAA ACTTGATCAA
    CCTGCCAATT TGCTACGGAT GTTTTTTGAT TGCCTGTATG ACGAGGAGGT GATCTCTGAG
    GATGCCTTCT ACAAGTGGGA GAGCAGCAAG GACCCGGCAG AGCAGAATGG GAAGGGTGTG
    GCCCTGAAGT CTGTCACGGC GTTCTTCACA TGGCTGCGGG AAGCAGAAGA GGAGTCTGAG
    GATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227921
    Clone ID Related Accession (Same CDS sequence) XM_024983027.1
    Accession Version XM_024983027.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4728bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATGGTTA ACCATCTGCC CATGCCGTAC CCAGTGCCCC AGGGTCCTCA GTACTGTATA 
    CCACAGTACC GTCACAGTGG CCCTCCGTAT GTTGGGCCAC CACAACAGTA CCCGGTTCAA
    CCACCAGGAC CAGGTCCTTT TTATCCTGGG CCAGGACCTG GGGACTTCCC CAGTGCTTAC
    GGAACGCCTT TTTACCCAAG TCAGCCAGTG TACCAGTCAG CACCTATCAT AGTGCCTACG
    CAGCAGCAGC CCCCTCCAGC CAAGAGAGAG AAAAAAACAA TAAGAATTCG GGATCCAAAC
    CAGGGAGGTA AAGACATAAC AGAGGAGATT ATGTCTGGAG GTGGCAGCCG AAATCCTACT
    CCACCCATAG GGAGACCCAC TTCCACACCT ACTCCTCCTC AGCAGCTGCC CAGCCAGGTC
    CCCGAGCACA GCCCTGTGGT CTATGGGACT GTGGAGAGCG CTCATCTTGC TGCCAGCACC
    CCTGTCACTG CAGCGAGCGA CCAGAAGCAA GAAGAGAAGC CAAAACCAGA TCCAGTGTTA
    AAGACTCCTT CCCCAGTCCT TAGGCTAGCC CTCAGTGGAG AGAAGAAAGA ACAAGCAGGC
    CCGACGTCTG AGATTACTGC AGTAGAGTCC ATACCAGAGC TTCCTCTGCC TCCATCCCCT
    ACTGCTATTT CTCCACTTGC TCGAAGTACA ATTACTTCTC CCACCTCTGT TGCTCTTAGT
    AGCCAACCAA TATTCACCAC TGCAATTGAT GACAGATGTG AACTCTCTTC CTCAAAAGAA
    GATACAATTC CTATTCCCAG CCCCACATCT TGCACAGAAG CATCAGATCC TTTACCAACA
    GATGAAATTG ATGATGATAT ATGCAAGAAA TCTTGTAGTG TAGCACCAAA TGATATTCCA
    CTGATTTCTA GTACTAACCT AATTAATGAA ATGAATGGAG TTAATGAAAA ATTACCAGCC
    ACGGAAAGCA TTGTGGAAAT AGTAAAACAG GAGGTGTTAC CGCTGACTCT TGAATTGGAG
    ATTTTGGAAA ATCCCCCTGA AGAAATCAAA GTGGAGTGTG TCCCAACTCC CATCACCCCT
    TCGATAGTTC CCTCCTCTTC TCCGTCTCCT CCAGCTCCTC CAGCTTCTCC TTCTCCCACA
    CCAGTTATCG TTCCTGCTGC TGCCACCAGT GTTAATACTG GTCGTGGTTC CACCGGAGTC
    CAGAGAGCCT TCGAAGAGGG CGAGAACATA AGAACTTGCC TTAGTGAAGA TGCAAAAGAG
    ATTCAGAACA AAGTAGAGGT AGAAGCAGAT GGGCAAACAG AAGAGATTGT GGATTCTCAG
    AACTTAAGTT CAAGAAAGAG CCCAGTCCCA GCTCAGACAG CTATACCTGC ACCAAAGATG
    TGGAAGAAAC CAAAAGACCG GACCCGAGCC ACTGAAGAGT CGTTAGAGGC AGAATCAGAG
    CCTAAAGCTG AAGAGGAGCT TTCAGGTGAC AAAATACTTG AATCTGAACA GGATAAAATG
    AGCCAAGGGT TTCATCCTGA AAGAGACCCC TCTGACCTAA AAAACGTGAA AACTGTGGAA
    GAAAATGGAG AAGAAGCTGA GCCTGTACGT AATGGTGCTG AGAGTATTTC TGAGGGTGAA
    GGAATAGATG CTAATTCAGG CTCCACTGAT AGTTCTGGTG AAGGGGTCAC ATTCCCATTT
    AAGTCAGAAT CCTGGAAGCC TCCTGATTCG GAAGGCAAGA AGCAGTATGA CAGGGAGTTT
    TTGCTGGATT TCCAATTCAT GCCAGCGTGT ATTCAGAAAC CAGAGGGCCT GCCTCCCATC
    AGTGATGTGG TTCTTGACAA GATCAATCAA CCAAAATTGC CAATGCGAAC TCTGGATCCT
    CGGATTTTGC CTCGAGGACC AGACTTTACT CCAGCCTTTG CAGATTTTGG AAGGCAAGCC
    CCTGGTGGAA GAGGTGTACC TGTTTGCAAA GTGCAGAGCA GGCATGGATT GCCAATTCTG
    GAACAGAGCA AAGCCCCAGC TTCCACTCCA CTGGCGATGT CGCACCCACC CCTGAAGAGC
    CTGCCTCTAG GGCTGTTGAA TGTTGGAGCA CGGAGATCTC AGCCTGGCCA GAGGAGAGAG
    CCCAGGAAGA TCATCACGGT CTCCGTGAAA GAAGACGTGC ACCTGAAGAA GGCAGAGAAC
    GCCTGGAAGC CCAGCCAGAG GCGGGATAGC CCAGCCGAGG ACCCCGAGAA CATCAAGACC
    CAGGAACTTT TTAGAAAAGT TCGAAGTATC TTAAATAAAT TGACACCACA GATGTTCAAC
    CAACTGATGA AGCAAGTGTC AGGACTTACT GTTGACACAG AGGAGCGGCT GAAAGGAGTC
    ATTGACCTGG TCTTTGAGAA GGCTATTGAC GAACCCAGTT TCTCTGTGGC TTACGCAAAC
    ATGTGTCGAT GTCTAGTAAC GCTGAAAGTA CCCATGGCAG ACAAGCCTGG TAACACAGTG
    AACTTCCGGA AGCTACTACT GAACCGTTGC CAGAAGGAAT TTGAAAAAGA TAAAGCCGAT
    GATGATGTCT TTGAGAAGAA GCAGAAAGAA CTCGAGGCTG CCAGTGCTCC AGAGGAGAGG
    ACAAGGCTTC ATGATGAACT GGAAGAAGCT AAAGATAAAG CCCGGCGGAG ATCCATTGGC
    AACATCAAGT TCATTGGAGA ACTCTTTAAA CTCAAAATGC TGACTGAAGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT GAAGAACCAC GATGAAGAAT CCCTCGAGTG TCTGTGTCGC
    CTGCTCACCA CAATTGGCAA AGACCTGGAC TTTGAGAAAG CAAAGCCACG TATGGACCAG
    TATTTTAATC AGATGGAGAA AATTGTAAAA GAAAGAAAAA CCTCATCTAG GATTCGATTC
    ATGCTTCAGG ACGTAATAGA TCTAAGGCTG TGTAATTGGG TATCACGACG AGCAGATCAG
    GGGCCTAAAA CTATTGAACA GATTCACAAA GAAGCTAAAA TAGAAGAACA GGAAGAGCAG
    AGGAAGGTCC AACAACTCAT GACCAAAGAG AAGAGAAGAC CAGGTGTCCA GAGAGTGGAT
    GAAGGTGGGT GGAACACTGT TCAAGGGGCC AAGAATAGTC GGGTATTGGA CCCCTCAAAA
    TTTCTGAAAA TCACTAAGCC CACAATTGAT GAGAAAATTC AGCTGGTACC TAAAGCGCAG
    CTGGGCAGCT GGGGAAAGGG CAGCAGTGGT GGAGCCAAGG CGAGCGAGGC CGATGCCTTA
    AGATCAAGTG CTTCCAGTTT AAACAGATTC TCTGCTTTGC AACCTCCAGC ACCTTCAGGG
    TCTGCGTCAT CCACACCTCT AGAGTTTGAT TCCCGGCGGA CCTTAACTAG TCGAGGAAGC
    ACGGGCAGGG AGAAGAATGA CAAGCCCCTC CAGCCTGCAG CAGCTCGGCC GAACACTTTC
    ATGAGGGGTG GCAGCAGTAA AGACCTGTTA GACAATCAGT CTCAGGAGGA GCAGCGGAGG
    GAGATGCTGG AGACCGTGAA GCAGCTCACA GGAGGCATGG ACGTGGAGAG GAGCAGCGCT
    GAGGCGGAGC GAAGCAGGAC CAGGGAGCCA GTGACAAAAC CGGAAATTCC AGTAACATCA
    GCCTCTGACA AGCCCGCGTT GTCAGAGGAA GAGATGGAGA GGAAGTCCAA ATCTATCATT
    GATGAGTTTC TACACATTAA TGATTTTAAG GAAGCCATGC AGTGTGTGGA AGAGCTGAAC
    GCCCAGGGCC TGCAACATGT GTTCGTGAGG ACGGGAGTGG AGTCCACCCT GGAAAGGAGC
    CAGATCACCC GGGATCACAT GGGCCAGTTA CTGTATCAGC TGGTGCAGTC AGAAAAGCTC
    AGCAAACAGG ACTTTTTCAG AGGTTTTTCA GAAACATTGG AATTGGCAGA TGACATGGCC
    ATTGATATTC CCCATATTTG GTTGTACCTT GCTGAACTGG TGACTCCCAT GTTAAAAGAA
    GGCGGAATCT CCATGAGACA ACTCATCACA GAATTTTGCA AACCTTTACT TCCTGTTGGA
    AGAGCTGGAA TCTTGATTTC TGAAATATTG CACCTTCTAT GCAAACAAAT GAGCCATAAG
    AAAGTGGGAG CCCTATGGAG GGAGGCTGAC CTCAGCTGGA AGGACTTTTT ACCAGAAGGG
    GAAGATGTAC ATAATTTTCT CTTGGAGCAG AAGTTGGACT TTTTAGAGTC TGACAGTTCC
    TGTTCCTCCG AAGCACTTTT GAAGAAAGAA CTCTCTGCCG AAGAGCTGTA TAAGCGACTC
    GAGAAACTCA TTATTGAGGA CAAGGCAAAT GATGAACAGA TCTTTGACTG GGTAGAGGCT
    AATCTAGATG AAAGCCAGAT GAGTTCACCT ACATTCCTTA GAGCTTTAAT GACAGCCGTC
    TGTAAAGCAG CTATTATAGC CGACTGTTCG ACCTTCCGAG TGGACACTGC TGTTATCAAG
    CAGAGAGTGC CGATCTTACT CAAGTACCTA GACTCAGATA CAGAGAAGGA ACTGCAAGCA
    CTTTATGCAC TACAAGCATC AATAGTAAAA CTTGATCAAC CTGCCAATTT GCTACGGATG
    TTTTTTGATT GCCTGTATGA CGAGGAGGTG ATCTCTGAGG ATGCCTTCTA CAAGTGGGAG
    AGCAGCAAGG ACCCGGCAGA GCAGAATGGG AAGGGTGTGG CCCTGAAGTC TGTCACGGCG
    TTCTTCACAT GGCTGCGGGA AGCAGAAGAG GAGTCTGAGG ATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838795.1
    CDS351..5078
    Translation

    Target ORF information:

    RefSeq Version XM_024983027.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983027.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATGGTTA ACCATCTGCC CATGCCGTAC CCAGTGCCCC AGGGTCCTCA GTACTGTATA 
    CCACAGTACC GTCACAGTGG CCCTCCGTAT GTTGGGCCAC CACAACAGTA CCCGGTTCAA
    CCACCAGGAC CAGGTCCTTT TTATCCTGGG CCAGGACCTG GGGACTTCCC CAGTGCTTAC
    GGAACGCCTT TTTACCCAAG TCAGCCAGTG TACCAGTCAG CACCTATCAT AGTGCCTACG
    CAGCAGCAGC CCCCTCCAGC CAAGAGAGAG AAAAAAACAA TAAGAATTCG GGATCCAAAC
    CAGGGAGGTA AAGACATAAC AGAGGAGATT ATGTCTGGAG GTGGCAGCCG AAATCCTACT
    CCACCCATAG GGAGACCCAC TTCCACACCT ACTCCTCCTC AGCAGCTGCC CAGCCAGGTC
    CCCGAGCACA GCCCTGTGGT CTATGGGACT GTGGAGAGCG CTCATCTTGC TGCCAGCACC
    CCTGTCACTG CAGCGAGCGA CCAGAAGCAA GAAGAGAAGC CAAAACCAGA TCCAGTGTTA
    AAGACTCCTT CCCCAGTCCT TAGGCTAGCC CTCAGTGGAG AGAAGAAAGA ACAAGCAGGC
    CCGACGTCTG AGATTACTGC AGTAGAGTCC ATACCAGAGC TTCCTCTGCC TCCATCCCCT
    ACTGCTATTT CTCCACTTGC TCGAAGTACA ATTACTTCTC CCACCTCTGT TGCTCTTAGT
    AGCCAACCAA TATTCACCAC TGCAATTGAT GACAGATGTG AACTCTCTTC CTCAAAAGAA
    GATACAATTC CTATTCCCAG CCCCACATCT TGCACAGAAG CATCAGATCC TTTACCAACA
    GATGAAATTG ATGATGATAT ATGCAAGAAA TCTTGTAGTG TAGCACCAAA TGATATTCCA
    CTGATTTCTA GTACTAACCT AATTAATGAA ATGAATGGAG TTAATGAAAA ATTACCAGCC
    ACGGAAAGCA TTGTGGAAAT AGTAAAACAG GAGGTGTTAC CGCTGACTCT TGAATTGGAG
    ATTTTGGAAA ATCCCCCTGA AGAAATCAAA GTGGAGTGTG TCCCAACTCC CATCACCCCT
    TCGATAGTTC CCTCCTCTTC TCCGTCTCCT CCAGCTCCTC CAGCTTCTCC TTCTCCCACA
    CCAGTTATCG TTCCTGCTGC TGCCACCAGT GTTAATACTG GTCGTGGTTC CACCGGAGTC
    CAGAGAGCCT TCGAAGAGGG CGAGAACATA AGAACTTGCC TTAGTGAAGA TGCAAAAGAG
    ATTCAGAACA AAGTAGAGGT AGAAGCAGAT GGGCAAACAG AAGAGATTGT GGATTCTCAG
    AACTTAAGTT CAAGAAAGAG CCCAGTCCCA GCTCAGACAG CTATACCTGC ACCAAAGATG
    TGGAAGAAAC CAAAAGACCG GACCCGAGCC ACTGAAGAGT CGTTAGAGGC AGAATCAGAG
    CCTAAAGCTG AAGAGGAGCT TTCAGGTGAC AAAATACTTG AATCTGAACA GGATAAAATG
    AGCCAAGGGT TTCATCCTGA AAGAGACCCC TCTGACCTAA AAAACGTGAA AACTGTGGAA
    GAAAATGGAG AAGAAGCTGA GCCTGTACGT AATGGTGCTG AGAGTATTTC TGAGGGTGAA
    GGAATAGATG CTAATTCAGG CTCCACTGAT AGTTCTGGTG AAGGGGTCAC ATTCCCATTT
    AAGTCAGAAT CCTGGAAGCC TCCTGATTCG GAAGGCAAGA AGCAGTATGA CAGGGAGTTT
    TTGCTGGATT TCCAATTCAT GCCAGCGTGT ATTCAGAAAC CAGAGGGCCT GCCTCCCATC
    AGTGATGTGG TTCTTGACAA GATCAATCAA CCAAAATTGC CAATGCGAAC TCTGGATCCT
    CGGATTTTGC CTCGAGGACC AGACTTTACT CCAGCCTTTG CAGATTTTGG AAGGCAAGCC
    CCTGGTGGAA GAGGTGTACC TGTTTGCAAA GTGCAGAGCA GGCATGGATT GCCAATTCTG
    GAACAGAGCA AAGCCCCAGC TTCCACTCCA CTGGCGATGT CGCACCCACC CCTGAAGAGC
    CTGCCTCTAG GGCTGTTGAA TGTTGGAGCA CGGAGATCTC AGCCTGGCCA GAGGAGAGAG
    CCCAGGAAGA TCATCACGGT CTCCGTGAAA GAAGACGTGC ACCTGAAGAA GGCAGAGAAC
    GCCTGGAAGC CCAGCCAGAG GCGGGATAGC CCAGCCGAGG ACCCCGAGAA CATCAAGACC
    CAGGAACTTT TTAGAAAAGT TCGAAGTATC TTAAATAAAT TGACACCACA GATGTTCAAC
    CAACTGATGA AGCAAGTGTC AGGACTTACT GTTGACACAG AGGAGCGGCT GAAAGGAGTC
    ATTGACCTGG TCTTTGAGAA GGCTATTGAC GAACCCAGTT TCTCTGTGGC TTACGCAAAC
    ATGTGTCGAT GTCTAGTAAC GCTGAAAGTA CCCATGGCAG ACAAGCCTGG TAACACAGTG
    AACTTCCGGA AGCTACTACT GAACCGTTGC CAGAAGGAAT TTGAAAAAGA TAAAGCCGAT
    GATGATGTCT TTGAGAAGAA GCAGAAAGAA CTCGAGGCTG CCAGTGCTCC AGAGGAGAGG
    ACAAGGCTTC ATGATGAACT GGAAGAAGCT AAAGATAAAG CCCGGCGGAG ATCCATTGGC
    AACATCAAGT TCATTGGAGA ACTCTTTAAA CTCAAAATGC TGACTGAAGC CATCATGCAT
    GACTGTGTGG TGAAGCTGCT GAAGAACCAC GATGAAGAAT CCCTCGAGTG TCTGTGTCGC
    CTGCTCACCA CAATTGGCAA AGACCTGGAC TTTGAGAAAG CAAAGCCACG TATGGACCAG
    TATTTTAATC AGATGGAGAA AATTGTAAAA GAAAGAAAAA CCTCATCTAG GATTCGATTC
    ATGCTTCAGG ACGTAATAGA TCTAAGGCTG TGTAATTGGG TATCACGACG AGCAGATCAG
    GGGCCTAAAA CTATTGAACA GATTCACAAA GAAGCTAAAA TAGAAGAACA GGAAGAGCAG
    AGGAAGGTCC AACAACTCAT GACCAAAGAG AAGAGAAGAC CAGGTGTCCA GAGAGTGGAT
    GAAGGTGGGT GGAACACTGT TCAAGGGGCC AAGAATAGTC GGGTATTGGA CCCCTCAAAA
    TTTCTGAAAA TCACTAAGCC CACAATTGAT GAGAAAATTC AGCTGGTACC TAAAGCGCAG
    CTGGGCAGCT GGGGAAAGGG CAGCAGTGGT GGAGCCAAGG CGAGCGAGGC CGATGCCTTA
    AGATCAAGTG CTTCCAGTTT AAACAGATTC TCTGCTTTGC AACCTCCAGC ACCTTCAGGG
    TCTGCGTCAT CCACACCTCT AGAGTTTGAT TCCCGGCGGA CCTTAACTAG TCGAGGAAGC
    ACGGGCAGGG AGAAGAATGA CAAGCCCCTC CAGCCTGCAG CAGCTCGGCC GAACACTTTC
    ATGAGGGGTG GCAGCAGTAA AGACCTGTTA GACAATCAGT CTCAGGAGGA GCAGCGGAGG
    GAGATGCTGG AGACCGTGAA GCAGCTCACA GGAGGCATGG ACGTGGAGAG GAGCAGCGCT
    GAGGCGGAGC GAAGCAGGAC CAGGGAGCCA GTGACAAAAC CGGAAATTCC AGTAACATCA
    GCCTCTGACA AGCCCGCGTT GTCAGAGGAA GAGATGGAGA GGAAGTCCAA ATCTATCATT
    GATGAGTTTC TACACATTAA TGATTTTAAG GAAGCCATGC AGTGTGTGGA AGAGCTGAAC
    GCCCAGGGCC TGCAACATGT GTTCGTGAGG ACGGGAGTGG AGTCCACCCT GGAAAGGAGC
    CAGATCACCC GGGATCACAT GGGCCAGTTA CTGTATCAGC TGGTGCAGTC AGAAAAGCTC
    AGCAAACAGG ACTTTTTCAG AGGTTTTTCA GAAACATTGG AATTGGCAGA TGACATGGCC
    ATTGATATTC CCCATATTTG GTTGTACCTT GCTGAACTGG TGACTCCCAT GTTAAAAGAA
    GGCGGAATCT CCATGAGACA ACTCATCACA GAATTTTGCA AACCTTTACT TCCTGTTGGA
    AGAGCTGGAA TCTTGATTTC TGAAATATTG CACCTTCTAT GCAAACAAAT GAGCCATAAG
    AAAGTGGGAG CCCTATGGAG GGAGGCTGAC CTCAGCTGGA AGGACTTTTT ACCAGAAGGG
    GAAGATGTAC ATAATTTTCT CTTGGAGCAG AAGTTGGACT TTTTAGAGTC TGACAGTTCC
    TGTTCCTCCG AAGCACTTTT GAAGAAAGAA CTCTCTGCCG AAGAGCTGTA TAAGCGACTC
    GAGAAACTCA TTATTGAGGA CAAGGCAAAT GATGAACAGA TCTTTGACTG GGTAGAGGCT
    AATCTAGATG AAAGCCAGAT GAGTTCACCT ACATTCCTTA GAGCTTTAAT GACAGCCGTC
    TGTAAAGCAG CTATTATAGC CGACTGTTCG ACCTTCCGAG TGGACACTGC TGTTATCAAG
    CAGAGAGTGC CGATCTTACT CAAGTACCTA GACTCAGATA CAGAGAAGGA ACTGCAAGCA
    CTTTATGCAC TACAAGCATC AATAGTAAAA CTTGATCAAC CTGCCAATTT GCTACGGATG
    TTTTTTGATT GCCTGTATGA CGAGGAGGTG ATCTCTGAGG ATGCCTTCTA CAAGTGGGAG
    AGCAGCAAGG ACCCGGCAGA GCAGAATGGG AAGGGTGTGG CCCTGAAGTC TGTCACGGCG
    TTCTTCACAT GGCTGCGGGA AGCAGAAGAG GAGTCTGAGG ATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88089
    Clone ID Related Accession (Same CDS sequence) XM_015460870.1 , XM_015460870.2 , XM_015462472.1
    Accession Version XM_015460870.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCTGCCC
    AGCCAGGTCC CCGAGCACAG CCCTGTGGTC TATGGGACTG TGGAGAGCGC TCATCTTGCT
    GCCAGCACCC CTGTCACTGC AGCGAGCGAC CAGAAGCAAG AAGAGAAGCC AAAACCAGAT
    CCAGTGTTAA AGACTCCTTC CCCAGTCCTT AGGCTAGCCC TCAGTGGAGA GAAGAAAGAA
    CAAGCAGGCC CGACGTCTGA GATTACTGCA GTAGAGTCCA TACCAGAGCT TCCTCTGCCT
    CCATCCCCTA CTGCTATTTC TCCACTTGCT CGAAGTACAA TTACTTCTCC CACCTCTGTT
    GCTCTTAGTA GCCAACCAAT ATTCACCACT GCAATTGATG ACAGATGTGA ACTCTCTTCC
    TCAAAAGAAG ATACAATTCC TATTCCCAGC CCCACATCTT GCACAGAAGC ATCAGATCCT
    TTACCAACAG ATGAAATTGA TGATGATATA TGCAAGAAAT CTTGTAGTGT AGCACCAAAT
    GATATTCCAC TGATTTCTAG TACTAACCTA ATTAATGAAA TGAATGGAGT TAATGAAAAA
    TTACCAGCCA CGGAAAGCAT TGTGGAAATA GTAAAACAGG AGGTGTTACC GCTGACTCTT
    GAATTGGAGA TTTTGGAAAA TCCCCCTGAA GAAATCAAAG TGGAGTGTGT CCCAACTCCC
    ATCACCCCTT CGATAGTTCC CTCCTCTTCT CCGTCTCCTC CAGCTCCTCC AGCTTCTCCT
    TCTCCCACAC CAGTTATCGT TCCTGCTGCT GCCACCAGTG TTAATACTGG TCGTGGTTCC
    ACCGGAGTCC AGAGAGCCTT CGAAGAGGGC GAGAACATAA GAACTTGCCT TAGTGAAGAT
    GCAAAAGAGA TTCAGAACAA AGTAGAGGTA GAAGCAGATG GGCAAACAGA AGAGATTGTG
    GATTCTCAGA ACTTAAGTTC AAGAAAGAGC CCAGTCCCAG CTCAGACAGC TATACCTGCA
    CCAAAGATGT GGAAGAAACC AAAAGACCGG ACCCGAGCCA CTGAAGAGTC GTTAGAGGCA
    GAATCAGAGC CTAAAGCTGA AGAGGAGCTT TCAGGTGACA AAATACTTGA ATCTGAACAG
    GATAAAATGA GCCAAGGGTT TCATCCTGAA AGAGACCCCT CTGACCTAAA AAACGTGAAA
    ACTGTGGAAG AAAATGGAGA AGAAGCTGAG CCTGTACGTA ATGGTGCTGA GAGTATTTCT
    GAGGGTGAAG GAATAGATGC TAATTCAGGC TCCACTGATA GTTCTGGTGA AGGGGTCACA
    TTCCCATTTA AGTCAGAATC CTGGAAGCCT CCTGATTCGG AAGGCAAGAA GCAGTATGAC
    AGGGAGTTTT TGCTGGATTT CCAATTCATG CCAGCGTGTA TTCAGAAACC AGAGGGCCTG
    CCTCCCATCA GTGATGTGGT TCTTGACAAG ATCAATCAAC CAAAATTGCC AATGCGAACT
    CTGGATCCTC GGATTTTGCC TCGAGGACCA GACTTTACTC CAGCCTTTGC AGATTTTGGA
    AGGCAAGCCC CTGGTGGAAG AGGTGTACCT GTTTGCAAAG TGCAGAGCAG GCATGGATTG
    CCAATTCTGG AACAGAGCAA AGCCCCAGCT TCCACTCCAC TGGCGATGTC GCACCCACCC
    CTGAAGAGCC TGCCTCTAGG GCTGTTGAAT GTTGGAGCAC GGAGATCTCA GCCTGGCCAG
    AGGAGAGAGC CCAGGAAGAT CATCACGGTC TCCGTGAAAG AAGACGTGCA CCTGAAGAAG
    GCAGAGAACG CCTGGAAGCC CAGCCAGAGG CGGGATAGCC CAGCCGAGGA CCCCGAGAAC
    ATCAAGACCC AGGAACTTTT TAGAAAAGTT CGAAGTATCT TAAATAAATT GACACCACAG
    ATGTTCAACC AACTGATGAA GCAAGTGTCA GGACTTACTG TTGACACAGA GGAGCGGCTG
    AAAGGAGTCA TTGACCTGGT CTTTGAGAAG GCTATTGACG AACCCAGTTT CTCTGTGGCT
    TACGCAAACA TGTGTCGATG TCTAGTAACG CTGAAAGTAC CCATGGCAGA CAAGCCTGGT
    AACACAGTGA ACTTCCGGAA GCTACTACTG AACCGTTGCC AGAAGGAATT TGAAAAAGAT
    AAAGCCGATG ATGATGTCTT TGAGAAGAAG CAGAAAGAAC TCGAGGCTGC CAGTGCTCCA
    GAGGAGAGGA CAAGGCTTCA TGATGAACTG GAAGAAGCTA AAGATAAAGC CCGGCGGAGA
    TCCATTGGCA ACATCAAGTT CATTGGAGAA CTCTTTAAAC TCAAAATGCT GACTGAAGCC
    ATCATGCATG ACTGTGTGGT GAAGCTGCTG AAGAACCACG ATGAAGAATC CCTCGAGTGT
    CTGTGTCGCC TGCTCACCAC AATTGGCAAA GACCTGGACT TTGAGAAAGC AAAGCCACGT
    ATGGACCAGT ATTTTAATCA GATGGAGAAA ATTGTAAAAG AAAGAAAAAC CTCATCTAGG
    ATTCGATTCA TGCTTCAGGA CGTAATAGAT CTAAGGCTGT GTAATTGGGT ATCACGACGA
    GCAGATCAGG GGCCTAAAAC TATTGAACAG ATTCACAAAG AAGCTAAAAT AGAAGAACAG
    GAAGAGCAGA GGAAGGTCCA ACAACTCATG ACCAAAGAGA AGAGAAGACC AGGTGTCCAG
    AGAGTGGATG AAGGTGGGTG GAACACTGTT CAAGGGGCCA AGAATAGTCG GGTATTGGAC
    CCCTCAAAAT TTCTGAAAAT CACTAAGCCC ACAATTGATG AGAAAATTCA GCTGGTACCT
    AAAGCGCAGC TGGGCAGCTG GGGAAAGGGC AGCAGTGGTG GAGCCAAGGC GAGCGAGGCC
    GATGCCTTAA GATCAAGTGC TTCCAGTTTA AACAGATTCT CTGCTTTGCA ACCTCCAGCA
    CCTTCAGGGT CTGCGTCATC CACACCTCTA GAGTTTGATT CCCGGCGGAC CTTAACTAGT
    CGAGGAAGCA CGGGCAGGGA GAAGAATGAC AAGCCCCTCC AGCCTGCAGC AGCTCGGCCG
    AACACTTTCA TGAGGGGTGG CAGCAGTAAA GACCTGTTAG ACAATCAGTC TCAGGAGGAG
    CAGCGGAGGG AGATGCTGGA GACCGTGAAG CAGCTCACAG GAGGCATGGA CGTGGAGAGG
    AGCAGCGCTG AGGCGGAGCG AAGCAGGACC AGGGAGCCAG TGACAAAACC GGAAATTCCA
    GTAACATCAG CCTCTGACAA GCCCGCGTTG TCAGAGGAAG AGATGGAGAG GAAGTCCAAA
    TCTATCATTG ATGAGTTTCT ACACATTAAT GATTTTAAGG AAGCCATGCA GTGTGTGGAA
    GAGCTGAACG CCCAGGGCCT GCAACATGTG TTCGTGAGGA CGGGAGTGGA GTCCACCCTG
    GAAAGGAGCC AGATCACCCG GGATCACATG GGCCAGTTAC TGTATCAGCT GGTGCAGTCA
    GAAAAGCTCA GCAAACAGGA CTTTTTCAGA GGTTTTTCAG AAACATTGGA ATTGGCAGAT
    GACATGGCCA TTGATATTCC CCATATTTGG TTGTACCTTG CTGAACTGGT GACTCCCATG
    TTAAAAGAAG GCGGAATCTC CATGAGACAA CTCATCACAG AATTTTGCAA ACCTTTACTT
    CCTGTTGGAA GAGCTGGAAT CTTGATTTCT GAAATATTGC ACCTTCTATG CAAACAAATG
    AGCCATAAGA AAGTGGGAGC CCTATGGAGG GAGGCTGACC TCAGCTGGAA GGACTTTTTA
    CCAGAAGGGG AAGATGTACA TAATTTTCTC TTGGAGCAGA AGTTGGACTT TTTAGAGTCT
    GACAGTTCCT GTTCCTCCGA AGCACTTTTG AAGAAAGAAC TCTCTGCCGA AGAGCTGTAT
    AAGCGACTCG AGAAACTCAT TATTGAGGAC AAGGCAAATG ATGAACAGAT CTTTGACTGG
    GTAGAGGCTA ATCTAGATGA AAGCCAGATG AGTTCACCTA CATTCCTTAG AGCTTTAATG
    ACAGCCGTCT GTAAAGCAGC TATTATAGCC GACTGTTCGA CCTTCCGAGT GGACACTGCT
    GTTATCAAGC AGAGAGTGCC GATCTTACTC AAGTACCTAG ACTCAGATAC AGAGAAGGAA
    CTGCAAGCAC TTTATGCACT ACAAGCATCA ATAGTAAAAC TTGATCAACC TGCCAATTTG
    CTACGGATGT TTTTTGATTG CCTGTATGAC GAGGAGGTGA TCTCTGAGGA TGCCTTCTAC
    AAGTGGGAGA GCAGCAAGGA CCCGGCAGAG CAGAATGGGA AGGGTGTGGC CCTGAAGTCT
    GTCACGGCGT TCTTCACATG GCTGCGGGAA GCAGAAGAGG AGTCTGAGGA TAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316356.1
    CDS162..5198
    Translation

    Target ORF information:

    RefSeq Version XM_015460870.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460870.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCTGCCC
    AGCCAGGTCC CCGAGCACAG CCCTGTGGTC TATGGGACTG TGGAGAGCGC TCATCTTGCT
    GCCAGCACCC CTGTCACTGC AGCGAGCGAC CAGAAGCAAG AAGAGAAGCC AAAACCAGAT
    CCAGTGTTAA AGACTCCTTC CCCAGTCCTT AGGCTAGCCC TCAGTGGAGA GAAGAAAGAA
    CAAGCAGGCC CGACGTCTGA GATTACTGCA GTAGAGTCCA TACCAGAGCT TCCTCTGCCT
    CCATCCCCTA CTGCTATTTC TCCACTTGCT CGAAGTACAA TTACTTCTCC CACCTCTGTT
    GCTCTTAGTA GCCAACCAAT ATTCACCACT GCAATTGATG ACAGATGTGA ACTCTCTTCC
    TCAAAAGAAG ATACAATTCC TATTCCCAGC CCCACATCTT GCACAGAAGC ATCAGATCCT
    TTACCAACAG ATGAAATTGA TGATGATATA TGCAAGAAAT CTTGTAGTGT AGCACCAAAT
    GATATTCCAC TGATTTCTAG TACTAACCTA ATTAATGAAA TGAATGGAGT TAATGAAAAA
    TTACCAGCCA CGGAAAGCAT TGTGGAAATA GTAAAACAGG AGGTGTTACC GCTGACTCTT
    GAATTGGAGA TTTTGGAAAA TCCCCCTGAA GAAATCAAAG TGGAGTGTGT CCCAACTCCC
    ATCACCCCTT CGATAGTTCC CTCCTCTTCT CCGTCTCCTC CAGCTCCTCC AGCTTCTCCT
    TCTCCCACAC CAGTTATCGT TCCTGCTGCT GCCACCAGTG TTAATACTGG TCGTGGTTCC
    ACCGGAGTCC AGAGAGCCTT CGAAGAGGGC GAGAACATAA GAACTTGCCT TAGTGAAGAT
    GCAAAAGAGA TTCAGAACAA AGTAGAGGTA GAAGCAGATG GGCAAACAGA AGAGATTGTG
    GATTCTCAGA ACTTAAGTTC AAGAAAGAGC CCAGTCCCAG CTCAGACAGC TATACCTGCA
    CCAAAGATGT GGAAGAAACC AAAAGACCGG ACCCGAGCCA CTGAAGAGTC GTTAGAGGCA
    GAATCAGAGC CTAAAGCTGA AGAGGAGCTT TCAGGTGACA AAATACTTGA ATCTGAACAG
    GATAAAATGA GCCAAGGGTT TCATCCTGAA AGAGACCCCT CTGACCTAAA AAACGTGAAA
    ACTGTGGAAG AAAATGGAGA AGAAGCTGAG CCTGTACGTA ATGGTGCTGA GAGTATTTCT
    GAGGGTGAAG GAATAGATGC TAATTCAGGC TCCACTGATA GTTCTGGTGA AGGGGTCACA
    TTCCCATTTA AGTCAGAATC CTGGAAGCCT CCTGATTCGG AAGGCAAGAA GCAGTATGAC
    AGGGAGTTTT TGCTGGATTT CCAATTCATG CCAGCGTGTA TTCAGAAACC AGAGGGCCTG
    CCTCCCATCA GTGATGTGGT TCTTGACAAG ATCAATCAAC CAAAATTGCC AATGCGAACT
    CTGGATCCTC GGATTTTGCC TCGAGGACCA GACTTTACTC CAGCCTTTGC AGATTTTGGA
    AGGCAAGCCC CTGGTGGAAG AGGTGTACCT GTTTGCAAAG TGCAGAGCAG GCATGGATTG
    CCAATTCTGG AACAGAGCAA AGCCCCAGCT TCCACTCCAC TGGCGATGTC GCACCCACCC
    CTGAAGAGCC TGCCTCTAGG GCTGTTGAAT GTTGGAGCAC GGAGATCTCA GCCTGGCCAG
    AGGAGAGAGC CCAGGAAGAT CATCACGGTC TCCGTGAAAG AAGACGTGCA CCTGAAGAAG
    GCAGAGAACG CCTGGAAGCC CAGCCAGAGG CGGGATAGCC CAGCCGAGGA CCCCGAGAAC
    ATCAAGACCC AGGAACTTTT TAGAAAAGTT CGAAGTATCT TAAATAAATT GACACCACAG
    ATGTTCAACC AACTGATGAA GCAAGTGTCA GGACTTACTG TTGACACAGA GGAGCGGCTG
    AAAGGAGTCA TTGACCTGGT CTTTGAGAAG GCTATTGACG AACCCAGTTT CTCTGTGGCT
    TACGCAAACA TGTGTCGATG TCTAGTAACG CTGAAAGTAC CCATGGCAGA CAAGCCTGGT
    AACACAGTGA ACTTCCGGAA GCTACTACTG AACCGTTGCC AGAAGGAATT TGAAAAAGAT
    AAAGCCGATG ATGATGTCTT TGAGAAGAAG CAGAAAGAAC TCGAGGCTGC CAGTGCTCCA
    GAGGAGAGGA CAAGGCTTCA TGATGAACTG GAAGAAGCTA AAGATAAAGC CCGGCGGAGA
    TCCATTGGCA ACATCAAGTT CATTGGAGAA CTCTTTAAAC TCAAAATGCT GACTGAAGCC
    ATCATGCATG ACTGTGTGGT GAAGCTGCTG AAGAACCACG ATGAAGAATC CCTCGAGTGT
    CTGTGTCGCC TGCTCACCAC AATTGGCAAA GACCTGGACT TTGAGAAAGC AAAGCCACGT
    ATGGACCAGT ATTTTAATCA GATGGAGAAA ATTGTAAAAG AAAGAAAAAC CTCATCTAGG
    ATTCGATTCA TGCTTCAGGA CGTAATAGAT CTAAGGCTGT GTAATTGGGT ATCACGACGA
    GCAGATCAGG GGCCTAAAAC TATTGAACAG ATTCACAAAG AAGCTAAAAT AGAAGAACAG
    GAAGAGCAGA GGAAGGTCCA ACAACTCATG ACCAAAGAGA AGAGAAGACC AGGTGTCCAG
    AGAGTGGATG AAGGTGGGTG GAACACTGTT CAAGGGGCCA AGAATAGTCG GGTATTGGAC
    CCCTCAAAAT TTCTGAAAAT CACTAAGCCC ACAATTGATG AGAAAATTCA GCTGGTACCT
    AAAGCGCAGC TGGGCAGCTG GGGAAAGGGC AGCAGTGGTG GAGCCAAGGC GAGCGAGGCC
    GATGCCTTAA GATCAAGTGC TTCCAGTTTA AACAGATTCT CTGCTTTGCA ACCTCCAGCA
    CCTTCAGGGT CTGCGTCATC CACACCTCTA GAGTTTGATT CCCGGCGGAC CTTAACTAGT
    CGAGGAAGCA CGGGCAGGGA GAAGAATGAC AAGCCCCTCC AGCCTGCAGC AGCTCGGCCG
    AACACTTTCA TGAGGGGTGG CAGCAGTAAA GACCTGTTAG ACAATCAGTC TCAGGAGGAG
    CAGCGGAGGG AGATGCTGGA GACCGTGAAG CAGCTCACAG GAGGCATGGA CGTGGAGAGG
    AGCAGCGCTG AGGCGGAGCG AAGCAGGACC AGGGAGCCAG TGACAAAACC GGAAATTCCA
    GTAACATCAG CCTCTGACAA GCCCGCGTTG TCAGAGGAAG AGATGGAGAG GAAGTCCAAA
    TCTATCATTG ATGAGTTTCT ACACATTAAT GATTTTAAGG AAGCCATGCA GTGTGTGGAA
    GAGCTGAACG CCCAGGGCCT GCAACATGTG TTCGTGAGGA CGGGAGTGGA GTCCACCCTG
    GAAAGGAGCC AGATCACCCG GGATCACATG GGCCAGTTAC TGTATCAGCT GGTGCAGTCA
    GAAAAGCTCA GCAAACAGGA CTTTTTCAGA GGTTTTTCAG AAACATTGGA ATTGGCAGAT
    GACATGGCCA TTGATATTCC CCATATTTGG TTGTACCTTG CTGAACTGGT GACTCCCATG
    TTAAAAGAAG GCGGAATCTC CATGAGACAA CTCATCACAG AATTTTGCAA ACCTTTACTT
    CCTGTTGGAA GAGCTGGAAT CTTGATTTCT GAAATATTGC ACCTTCTATG CAAACAAATG
    AGCCATAAGA AAGTGGGAGC CCTATGGAGG GAGGCTGACC TCAGCTGGAA GGACTTTTTA
    CCAGAAGGGG AAGATGTACA TAATTTTCTC TTGGAGCAGA AGTTGGACTT TTTAGAGTCT
    GACAGTTCCT GTTCCTCCGA AGCACTTTTG AAGAAAGAAC TCTCTGCCGA AGAGCTGTAT
    AAGCGACTCG AGAAACTCAT TATTGAGGAC AAGGCAAATG ATGAACAGAT CTTTGACTGG
    GTAGAGGCTA ATCTAGATGA AAGCCAGATG AGTTCACCTA CATTCCTTAG AGCTTTAATG
    ACAGCCGTCT GTAAAGCAGC TATTATAGCC GACTGTTCGA CCTTCCGAGT GGACACTGCT
    GTTATCAAGC AGAGAGTGCC GATCTTACTC AAGTACCTAG ACTCAGATAC AGAGAAGGAA
    CTGCAAGCAC TTTATGCACT ACAAGCATCA ATAGTAAAAC TTGATCAACC TGCCAATTTG
    CTACGGATGT TTTTTGATTG CCTGTATGAC GAGGAGGTGA TCTCTGAGGA TGCCTTCTAC
    AAGTGGGAGA GCAGCAAGGA CCCGGCAGAG CAGAATGGGA AGGGTGTGGC CCTGAAGTCT
    GTCACGGCGT TCTTCACATG GCTGCGGGAA GCAGAAGAGG AGTCTGAGGA TAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88089
    Clone ID Related Accession (Same CDS sequence) XM_015460870.1 , XM_015460870.2 , XM_015462472.1
    Accession Version XM_015460870.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCTGCCC
    AGCCAGGTCC CCGAGCACAG CCCTGTGGTC TATGGGACTG TGGAGAGCGC TCATCTTGCT
    GCCAGCACCC CTGTCACTGC AGCGAGCGAC CAGAAGCAAG AAGAGAAGCC AAAACCAGAT
    CCAGTGTTAA AGACTCCTTC CCCAGTCCTT AGGCTAGCCC TCAGTGGAGA GAAGAAAGAA
    CAAGCAGGCC CGACGTCTGA GATTACTGCA GTAGAGTCCA TACCAGAGCT TCCTCTGCCT
    CCATCCCCTA CTGCTATTTC TCCACTTGCT CGAAGTACAA TTACTTCTCC CACCTCTGTT
    GCTCTTAGTA GCCAACCAAT ATTCACCACT GCAATTGATG ACAGATGTGA ACTCTCTTCC
    TCAAAAGAAG ATACAATTCC TATTCCCAGC CCCACATCTT GCACAGAAGC ATCAGATCCT
    TTACCAACAG ATGAAATTGA TGATGATATA TGCAAGAAAT CTTGTAGTGT AGCACCAAAT
    GATATTCCAC TGATTTCTAG TACTAACCTA ATTAATGAAA TGAATGGAGT TAATGAAAAA
    TTACCAGCCA CGGAAAGCAT TGTGGAAATA GTAAAACAGG AGGTGTTACC GCTGACTCTT
    GAATTGGAGA TTTTGGAAAA TCCCCCTGAA GAAATCAAAG TGGAGTGTGT CCCAACTCCC
    ATCACCCCTT CGATAGTTCC CTCCTCTTCT CCGTCTCCTC CAGCTCCTCC AGCTTCTCCT
    TCTCCCACAC CAGTTATCGT TCCTGCTGCT GCCACCAGTG TTAATACTGG TCGTGGTTCC
    ACCGGAGTCC AGAGAGCCTT CGAAGAGGGC GAGAACATAA GAACTTGCCT TAGTGAAGAT
    GCAAAAGAGA TTCAGAACAA AGTAGAGGTA GAAGCAGATG GGCAAACAGA AGAGATTGTG
    GATTCTCAGA ACTTAAGTTC AAGAAAGAGC CCAGTCCCAG CTCAGACAGC TATACCTGCA
    CCAAAGATGT GGAAGAAACC AAAAGACCGG ACCCGAGCCA CTGAAGAGTC GTTAGAGGCA
    GAATCAGAGC CTAAAGCTGA AGAGGAGCTT TCAGGTGACA AAATACTTGA ATCTGAACAG
    GATAAAATGA GCCAAGGGTT TCATCCTGAA AGAGACCCCT CTGACCTAAA AAACGTGAAA
    ACTGTGGAAG AAAATGGAGA AGAAGCTGAG CCTGTACGTA ATGGTGCTGA GAGTATTTCT
    GAGGGTGAAG GAATAGATGC TAATTCAGGC TCCACTGATA GTTCTGGTGA AGGGGTCACA
    TTCCCATTTA AGTCAGAATC CTGGAAGCCT CCTGATTCGG AAGGCAAGAA GCAGTATGAC
    AGGGAGTTTT TGCTGGATTT CCAATTCATG CCAGCGTGTA TTCAGAAACC AGAGGGCCTG
    CCTCCCATCA GTGATGTGGT TCTTGACAAG ATCAATCAAC CAAAATTGCC AATGCGAACT
    CTGGATCCTC GGATTTTGCC TCGAGGACCA GACTTTACTC CAGCCTTTGC AGATTTTGGA
    AGGCAAGCCC CTGGTGGAAG AGGTGTACCT GTTTGCAAAG TGCAGAGCAG GCATGGATTG
    CCAATTCTGG AACAGAGCAA AGCCCCAGCT TCCACTCCAC TGGCGATGTC GCACCCACCC
    CTGAAGAGCC TGCCTCTAGG GCTGTTGAAT GTTGGAGCAC GGAGATCTCA GCCTGGCCAG
    AGGAGAGAGC CCAGGAAGAT CATCACGGTC TCCGTGAAAG AAGACGTGCA CCTGAAGAAG
    GCAGAGAACG CCTGGAAGCC CAGCCAGAGG CGGGATAGCC CAGCCGAGGA CCCCGAGAAC
    ATCAAGACCC AGGAACTTTT TAGAAAAGTT CGAAGTATCT TAAATAAATT GACACCACAG
    ATGTTCAACC AACTGATGAA GCAAGTGTCA GGACTTACTG TTGACACAGA GGAGCGGCTG
    AAAGGAGTCA TTGACCTGGT CTTTGAGAAG GCTATTGACG AACCCAGTTT CTCTGTGGCT
    TACGCAAACA TGTGTCGATG TCTAGTAACG CTGAAAGTAC CCATGGCAGA CAAGCCTGGT
    AACACAGTGA ACTTCCGGAA GCTACTACTG AACCGTTGCC AGAAGGAATT TGAAAAAGAT
    AAAGCCGATG ATGATGTCTT TGAGAAGAAG CAGAAAGAAC TCGAGGCTGC CAGTGCTCCA
    GAGGAGAGGA CAAGGCTTCA TGATGAACTG GAAGAAGCTA AAGATAAAGC CCGGCGGAGA
    TCCATTGGCA ACATCAAGTT CATTGGAGAA CTCTTTAAAC TCAAAATGCT GACTGAAGCC
    ATCATGCATG ACTGTGTGGT GAAGCTGCTG AAGAACCACG ATGAAGAATC CCTCGAGTGT
    CTGTGTCGCC TGCTCACCAC AATTGGCAAA GACCTGGACT TTGAGAAAGC AAAGCCACGT
    ATGGACCAGT ATTTTAATCA GATGGAGAAA ATTGTAAAAG AAAGAAAAAC CTCATCTAGG
    ATTCGATTCA TGCTTCAGGA CGTAATAGAT CTAAGGCTGT GTAATTGGGT ATCACGACGA
    GCAGATCAGG GGCCTAAAAC TATTGAACAG ATTCACAAAG AAGCTAAAAT AGAAGAACAG
    GAAGAGCAGA GGAAGGTCCA ACAACTCATG ACCAAAGAGA AGAGAAGACC AGGTGTCCAG
    AGAGTGGATG AAGGTGGGTG GAACACTGTT CAAGGGGCCA AGAATAGTCG GGTATTGGAC
    CCCTCAAAAT TTCTGAAAAT CACTAAGCCC ACAATTGATG AGAAAATTCA GCTGGTACCT
    AAAGCGCAGC TGGGCAGCTG GGGAAAGGGC AGCAGTGGTG GAGCCAAGGC GAGCGAGGCC
    GATGCCTTAA GATCAAGTGC TTCCAGTTTA AACAGATTCT CTGCTTTGCA ACCTCCAGCA
    CCTTCAGGGT CTGCGTCATC CACACCTCTA GAGTTTGATT CCCGGCGGAC CTTAACTAGT
    CGAGGAAGCA CGGGCAGGGA GAAGAATGAC AAGCCCCTCC AGCCTGCAGC AGCTCGGCCG
    AACACTTTCA TGAGGGGTGG CAGCAGTAAA GACCTGTTAG ACAATCAGTC TCAGGAGGAG
    CAGCGGAGGG AGATGCTGGA GACCGTGAAG CAGCTCACAG GAGGCATGGA CGTGGAGAGG
    AGCAGCGCTG AGGCGGAGCG AAGCAGGACC AGGGAGCCAG TGACAAAACC GGAAATTCCA
    GTAACATCAG CCTCTGACAA GCCCGCGTTG TCAGAGGAAG AGATGGAGAG GAAGTCCAAA
    TCTATCATTG ATGAGTTTCT ACACATTAAT GATTTTAAGG AAGCCATGCA GTGTGTGGAA
    GAGCTGAACG CCCAGGGCCT GCAACATGTG TTCGTGAGGA CGGGAGTGGA GTCCACCCTG
    GAAAGGAGCC AGATCACCCG GGATCACATG GGCCAGTTAC TGTATCAGCT GGTGCAGTCA
    GAAAAGCTCA GCAAACAGGA CTTTTTCAGA GGTTTTTCAG AAACATTGGA ATTGGCAGAT
    GACATGGCCA TTGATATTCC CCATATTTGG TTGTACCTTG CTGAACTGGT GACTCCCATG
    TTAAAAGAAG GCGGAATCTC CATGAGACAA CTCATCACAG AATTTTGCAA ACCTTTACTT
    CCTGTTGGAA GAGCTGGAAT CTTGATTTCT GAAATATTGC ACCTTCTATG CAAACAAATG
    AGCCATAAGA AAGTGGGAGC CCTATGGAGG GAGGCTGACC TCAGCTGGAA GGACTTTTTA
    CCAGAAGGGG AAGATGTACA TAATTTTCTC TTGGAGCAGA AGTTGGACTT TTTAGAGTCT
    GACAGTTCCT GTTCCTCCGA AGCACTTTTG AAGAAAGAAC TCTCTGCCGA AGAGCTGTAT
    AAGCGACTCG AGAAACTCAT TATTGAGGAC AAGGCAAATG ATGAACAGAT CTTTGACTGG
    GTAGAGGCTA ATCTAGATGA AAGCCAGATG AGTTCACCTA CATTCCTTAG AGCTTTAATG
    ACAGCCGTCT GTAAAGCAGC TATTATAGCC GACTGTTCGA CCTTCCGAGT GGACACTGCT
    GTTATCAAGC AGAGAGTGCC GATCTTACTC AAGTACCTAG ACTCAGATAC AGAGAAGGAA
    CTGCAAGCAC TTTATGCACT ACAAGCATCA ATAGTAAAAC TTGATCAACC TGCCAATTTG
    CTACGGATGT TTTTTGATTG CCTGTATGAC GAGGAGGTGA TCTCTGAGGA TGCCTTCTAC
    AAGTGGGAGA GCAGCAAGGA CCCGGCAGAG CAGAATGGGA AGGGTGTGGC CCTGAAGTCT
    GTCACGGCGT TCTTCACATG GCTGCGGGAA GCAGAAGAGG AGTCTGAGGA TAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316356.1
    CDS196..5232
    Translation

    Target ORF information:

    RefSeq Version XM_015460870.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460870.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCTGCCC
    AGCCAGGTCC CCGAGCACAG CCCTGTGGTC TATGGGACTG TGGAGAGCGC TCATCTTGCT
    GCCAGCACCC CTGTCACTGC AGCGAGCGAC CAGAAGCAAG AAGAGAAGCC AAAACCAGAT
    CCAGTGTTAA AGACTCCTTC CCCAGTCCTT AGGCTAGCCC TCAGTGGAGA GAAGAAAGAA
    CAAGCAGGCC CGACGTCTGA GATTACTGCA GTAGAGTCCA TACCAGAGCT TCCTCTGCCT
    CCATCCCCTA CTGCTATTTC TCCACTTGCT CGAAGTACAA TTACTTCTCC CACCTCTGTT
    GCTCTTAGTA GCCAACCAAT ATTCACCACT GCAATTGATG ACAGATGTGA ACTCTCTTCC
    TCAAAAGAAG ATACAATTCC TATTCCCAGC CCCACATCTT GCACAGAAGC ATCAGATCCT
    TTACCAACAG ATGAAATTGA TGATGATATA TGCAAGAAAT CTTGTAGTGT AGCACCAAAT
    GATATTCCAC TGATTTCTAG TACTAACCTA ATTAATGAAA TGAATGGAGT TAATGAAAAA
    TTACCAGCCA CGGAAAGCAT TGTGGAAATA GTAAAACAGG AGGTGTTACC GCTGACTCTT
    GAATTGGAGA TTTTGGAAAA TCCCCCTGAA GAAATCAAAG TGGAGTGTGT CCCAACTCCC
    ATCACCCCTT CGATAGTTCC CTCCTCTTCT CCGTCTCCTC CAGCTCCTCC AGCTTCTCCT
    TCTCCCACAC CAGTTATCGT TCCTGCTGCT GCCACCAGTG TTAATACTGG TCGTGGTTCC
    ACCGGAGTCC AGAGAGCCTT CGAAGAGGGC GAGAACATAA GAACTTGCCT TAGTGAAGAT
    GCAAAAGAGA TTCAGAACAA AGTAGAGGTA GAAGCAGATG GGCAAACAGA AGAGATTGTG
    GATTCTCAGA ACTTAAGTTC AAGAAAGAGC CCAGTCCCAG CTCAGACAGC TATACCTGCA
    CCAAAGATGT GGAAGAAACC AAAAGACCGG ACCCGAGCCA CTGAAGAGTC GTTAGAGGCA
    GAATCAGAGC CTAAAGCTGA AGAGGAGCTT TCAGGTGACA AAATACTTGA ATCTGAACAG
    GATAAAATGA GCCAAGGGTT TCATCCTGAA AGAGACCCCT CTGACCTAAA AAACGTGAAA
    ACTGTGGAAG AAAATGGAGA AGAAGCTGAG CCTGTACGTA ATGGTGCTGA GAGTATTTCT
    GAGGGTGAAG GAATAGATGC TAATTCAGGC TCCACTGATA GTTCTGGTGA AGGGGTCACA
    TTCCCATTTA AGTCAGAATC CTGGAAGCCT CCTGATTCGG AAGGCAAGAA GCAGTATGAC
    AGGGAGTTTT TGCTGGATTT CCAATTCATG CCAGCGTGTA TTCAGAAACC AGAGGGCCTG
    CCTCCCATCA GTGATGTGGT TCTTGACAAG ATCAATCAAC CAAAATTGCC AATGCGAACT
    CTGGATCCTC GGATTTTGCC TCGAGGACCA GACTTTACTC CAGCCTTTGC AGATTTTGGA
    AGGCAAGCCC CTGGTGGAAG AGGTGTACCT GTTTGCAAAG TGCAGAGCAG GCATGGATTG
    CCAATTCTGG AACAGAGCAA AGCCCCAGCT TCCACTCCAC TGGCGATGTC GCACCCACCC
    CTGAAGAGCC TGCCTCTAGG GCTGTTGAAT GTTGGAGCAC GGAGATCTCA GCCTGGCCAG
    AGGAGAGAGC CCAGGAAGAT CATCACGGTC TCCGTGAAAG AAGACGTGCA CCTGAAGAAG
    GCAGAGAACG CCTGGAAGCC CAGCCAGAGG CGGGATAGCC CAGCCGAGGA CCCCGAGAAC
    ATCAAGACCC AGGAACTTTT TAGAAAAGTT CGAAGTATCT TAAATAAATT GACACCACAG
    ATGTTCAACC AACTGATGAA GCAAGTGTCA GGACTTACTG TTGACACAGA GGAGCGGCTG
    AAAGGAGTCA TTGACCTGGT CTTTGAGAAG GCTATTGACG AACCCAGTTT CTCTGTGGCT
    TACGCAAACA TGTGTCGATG TCTAGTAACG CTGAAAGTAC CCATGGCAGA CAAGCCTGGT
    AACACAGTGA ACTTCCGGAA GCTACTACTG AACCGTTGCC AGAAGGAATT TGAAAAAGAT
    AAAGCCGATG ATGATGTCTT TGAGAAGAAG CAGAAAGAAC TCGAGGCTGC CAGTGCTCCA
    GAGGAGAGGA CAAGGCTTCA TGATGAACTG GAAGAAGCTA AAGATAAAGC CCGGCGGAGA
    TCCATTGGCA ACATCAAGTT CATTGGAGAA CTCTTTAAAC TCAAAATGCT GACTGAAGCC
    ATCATGCATG ACTGTGTGGT GAAGCTGCTG AAGAACCACG ATGAAGAATC CCTCGAGTGT
    CTGTGTCGCC TGCTCACCAC AATTGGCAAA GACCTGGACT TTGAGAAAGC AAAGCCACGT
    ATGGACCAGT ATTTTAATCA GATGGAGAAA ATTGTAAAAG AAAGAAAAAC CTCATCTAGG
    ATTCGATTCA TGCTTCAGGA CGTAATAGAT CTAAGGCTGT GTAATTGGGT ATCACGACGA
    GCAGATCAGG GGCCTAAAAC TATTGAACAG ATTCACAAAG AAGCTAAAAT AGAAGAACAG
    GAAGAGCAGA GGAAGGTCCA ACAACTCATG ACCAAAGAGA AGAGAAGACC AGGTGTCCAG
    AGAGTGGATG AAGGTGGGTG GAACACTGTT CAAGGGGCCA AGAATAGTCG GGTATTGGAC
    CCCTCAAAAT TTCTGAAAAT CACTAAGCCC ACAATTGATG AGAAAATTCA GCTGGTACCT
    AAAGCGCAGC TGGGCAGCTG GGGAAAGGGC AGCAGTGGTG GAGCCAAGGC GAGCGAGGCC
    GATGCCTTAA GATCAAGTGC TTCCAGTTTA AACAGATTCT CTGCTTTGCA ACCTCCAGCA
    CCTTCAGGGT CTGCGTCATC CACACCTCTA GAGTTTGATT CCCGGCGGAC CTTAACTAGT
    CGAGGAAGCA CGGGCAGGGA GAAGAATGAC AAGCCCCTCC AGCCTGCAGC AGCTCGGCCG
    AACACTTTCA TGAGGGGTGG CAGCAGTAAA GACCTGTTAG ACAATCAGTC TCAGGAGGAG
    CAGCGGAGGG AGATGCTGGA GACCGTGAAG CAGCTCACAG GAGGCATGGA CGTGGAGAGG
    AGCAGCGCTG AGGCGGAGCG AAGCAGGACC AGGGAGCCAG TGACAAAACC GGAAATTCCA
    GTAACATCAG CCTCTGACAA GCCCGCGTTG TCAGAGGAAG AGATGGAGAG GAAGTCCAAA
    TCTATCATTG ATGAGTTTCT ACACATTAAT GATTTTAAGG AAGCCATGCA GTGTGTGGAA
    GAGCTGAACG CCCAGGGCCT GCAACATGTG TTCGTGAGGA CGGGAGTGGA GTCCACCCTG
    GAAAGGAGCC AGATCACCCG GGATCACATG GGCCAGTTAC TGTATCAGCT GGTGCAGTCA
    GAAAAGCTCA GCAAACAGGA CTTTTTCAGA GGTTTTTCAG AAACATTGGA ATTGGCAGAT
    GACATGGCCA TTGATATTCC CCATATTTGG TTGTACCTTG CTGAACTGGT GACTCCCATG
    TTAAAAGAAG GCGGAATCTC CATGAGACAA CTCATCACAG AATTTTGCAA ACCTTTACTT
    CCTGTTGGAA GAGCTGGAAT CTTGATTTCT GAAATATTGC ACCTTCTATG CAAACAAATG
    AGCCATAAGA AAGTGGGAGC CCTATGGAGG GAGGCTGACC TCAGCTGGAA GGACTTTTTA
    CCAGAAGGGG AAGATGTACA TAATTTTCTC TTGGAGCAGA AGTTGGACTT TTTAGAGTCT
    GACAGTTCCT GTTCCTCCGA AGCACTTTTG AAGAAAGAAC TCTCTGCCGA AGAGCTGTAT
    AAGCGACTCG AGAAACTCAT TATTGAGGAC AAGGCAAATG ATGAACAGAT CTTTGACTGG
    GTAGAGGCTA ATCTAGATGA AAGCCAGATG AGTTCACCTA CATTCCTTAG AGCTTTAATG
    ACAGCCGTCT GTAAAGCAGC TATTATAGCC GACTGTTCGA CCTTCCGAGT GGACACTGCT
    GTTATCAAGC AGAGAGTGCC GATCTTACTC AAGTACCTAG ACTCAGATAC AGAGAAGGAA
    CTGCAAGCAC TTTATGCACT ACAAGCATCA ATAGTAAAAC TTGATCAACC TGCCAATTTG
    CTACGGATGT TTTTTGATTG CCTGTATGAC GAGGAGGTGA TCTCTGAGGA TGCCTTCTAC
    AAGTGGGAGA GCAGCAAGGA CCCGGCAGAG CAGAATGGGA AGGGTGTGGC CCTGAAGTCT
    GTCACGGCGT TCTTCACATG GCTGCGGGAA GCAGAAGAGG AGTCTGAGGA TAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88089
    Clone ID Related Accession (Same CDS sequence) XM_015460870.1 , XM_015460870.2 , XM_015462472.1
    Accession Version XM_015462472.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCTGCCC
    AGCCAGGTCC CCGAGCACAG CCCTGTGGTC TATGGGACTG TGGAGAGCGC TCATCTTGCT
    GCCAGCACCC CTGTCACTGC AGCGAGCGAC CAGAAGCAAG AAGAGAAGCC AAAACCAGAT
    CCAGTGTTAA AGACTCCTTC CCCAGTCCTT AGGCTAGCCC TCAGTGGAGA GAAGAAAGAA
    CAAGCAGGCC CGACGTCTGA GATTACTGCA GTAGAGTCCA TACCAGAGCT TCCTCTGCCT
    CCATCCCCTA CTGCTATTTC TCCACTTGCT CGAAGTACAA TTACTTCTCC CACCTCTGTT
    GCTCTTAGTA GCCAACCAAT ATTCACCACT GCAATTGATG ACAGATGTGA ACTCTCTTCC
    TCAAAAGAAG ATACAATTCC TATTCCCAGC CCCACATCTT GCACAGAAGC ATCAGATCCT
    TTACCAACAG ATGAAATTGA TGATGATATA TGCAAGAAAT CTTGTAGTGT AGCACCAAAT
    GATATTCCAC TGATTTCTAG TACTAACCTA ATTAATGAAA TGAATGGAGT TAATGAAAAA
    TTACCAGCCA CGGAAAGCAT TGTGGAAATA GTAAAACAGG AGGTGTTACC GCTGACTCTT
    GAATTGGAGA TTTTGGAAAA TCCCCCTGAA GAAATCAAAG TGGAGTGTGT CCCAACTCCC
    ATCACCCCTT CGATAGTTCC CTCCTCTTCT CCGTCTCCTC CAGCTCCTCC AGCTTCTCCT
    TCTCCCACAC CAGTTATCGT TCCTGCTGCT GCCACCAGTG TTAATACTGG TCGTGGTTCC
    ACCGGAGTCC AGAGAGCCTT CGAAGAGGGC GAGAACATAA GAACTTGCCT TAGTGAAGAT
    GCAAAAGAGA TTCAGAACAA AGTAGAGGTA GAAGCAGATG GGCAAACAGA AGAGATTGTG
    GATTCTCAGA ACTTAAGTTC AAGAAAGAGC CCAGTCCCAG CTCAGACAGC TATACCTGCA
    CCAAAGATGT GGAAGAAACC AAAAGACCGG ACCCGAGCCA CTGAAGAGTC GTTAGAGGCA
    GAATCAGAGC CTAAAGCTGA AGAGGAGCTT TCAGGTGACA AAATACTTGA ATCTGAACAG
    GATAAAATGA GCCAAGGGTT TCATCCTGAA AGAGACCCCT CTGACCTAAA AAACGTGAAA
    ACTGTGGAAG AAAATGGAGA AGAAGCTGAG CCTGTACGTA ATGGTGCTGA GAGTATTTCT
    GAGGGTGAAG GAATAGATGC TAATTCAGGC TCCACTGATA GTTCTGGTGA AGGGGTCACA
    TTCCCATTTA AGTCAGAATC CTGGAAGCCT CCTGATTCGG AAGGCAAGAA GCAGTATGAC
    AGGGAGTTTT TGCTGGATTT CCAATTCATG CCAGCGTGTA TTCAGAAACC AGAGGGCCTG
    CCTCCCATCA GTGATGTGGT TCTTGACAAG ATCAATCAAC CAAAATTGCC AATGCGAACT
    CTGGATCCTC GGATTTTGCC TCGAGGACCA GACTTTACTC CAGCCTTTGC AGATTTTGGA
    AGGCAAGCCC CTGGTGGAAG AGGTGTACCT GTTTGCAAAG TGCAGAGCAG GCATGGATTG
    CCAATTCTGG AACAGAGCAA AGCCCCAGCT TCCACTCCAC TGGCGATGTC GCACCCACCC
    CTGAAGAGCC TGCCTCTAGG GCTGTTGAAT GTTGGAGCAC GGAGATCTCA GCCTGGCCAG
    AGGAGAGAGC CCAGGAAGAT CATCACGGTC TCCGTGAAAG AAGACGTGCA CCTGAAGAAG
    GCAGAGAACG CCTGGAAGCC CAGCCAGAGG CGGGATAGCC CAGCCGAGGA CCCCGAGAAC
    ATCAAGACCC AGGAACTTTT TAGAAAAGTT CGAAGTATCT TAAATAAATT GACACCACAG
    ATGTTCAACC AACTGATGAA GCAAGTGTCA GGACTTACTG TTGACACAGA GGAGCGGCTG
    AAAGGAGTCA TTGACCTGGT CTTTGAGAAG GCTATTGACG AACCCAGTTT CTCTGTGGCT
    TACGCAAACA TGTGTCGATG TCTAGTAACG CTGAAAGTAC CCATGGCAGA CAAGCCTGGT
    AACACAGTGA ACTTCCGGAA GCTACTACTG AACCGTTGCC AGAAGGAATT TGAAAAAGAT
    AAAGCCGATG ATGATGTCTT TGAGAAGAAG CAGAAAGAAC TCGAGGCTGC CAGTGCTCCA
    GAGGAGAGGA CAAGGCTTCA TGATGAACTG GAAGAAGCTA AAGATAAAGC CCGGCGGAGA
    TCCATTGGCA ACATCAAGTT CATTGGAGAA CTCTTTAAAC TCAAAATGCT GACTGAAGCC
    ATCATGCATG ACTGTGTGGT GAAGCTGCTG AAGAACCACG ATGAAGAATC CCTCGAGTGT
    CTGTGTCGCC TGCTCACCAC AATTGGCAAA GACCTGGACT TTGAGAAAGC AAAGCCACGT
    ATGGACCAGT ATTTTAATCA GATGGAGAAA ATTGTAAAAG AAAGAAAAAC CTCATCTAGG
    ATTCGATTCA TGCTTCAGGA CGTAATAGAT CTAAGGCTGT GTAATTGGGT ATCACGACGA
    GCAGATCAGG GGCCTAAAAC TATTGAACAG ATTCACAAAG AAGCTAAAAT AGAAGAACAG
    GAAGAGCAGA GGAAGGTCCA ACAACTCATG ACCAAAGAGA AGAGAAGACC AGGTGTCCAG
    AGAGTGGATG AAGGTGGGTG GAACACTGTT CAAGGGGCCA AGAATAGTCG GGTATTGGAC
    CCCTCAAAAT TTCTGAAAAT CACTAAGCCC ACAATTGATG AGAAAATTCA GCTGGTACCT
    AAAGCGCAGC TGGGCAGCTG GGGAAAGGGC AGCAGTGGTG GAGCCAAGGC GAGCGAGGCC
    GATGCCTTAA GATCAAGTGC TTCCAGTTTA AACAGATTCT CTGCTTTGCA ACCTCCAGCA
    CCTTCAGGGT CTGCGTCATC CACACCTCTA GAGTTTGATT CCCGGCGGAC CTTAACTAGT
    CGAGGAAGCA CGGGCAGGGA GAAGAATGAC AAGCCCCTCC AGCCTGCAGC AGCTCGGCCG
    AACACTTTCA TGAGGGGTGG CAGCAGTAAA GACCTGTTAG ACAATCAGTC TCAGGAGGAG
    CAGCGGAGGG AGATGCTGGA GACCGTGAAG CAGCTCACAG GAGGCATGGA CGTGGAGAGG
    AGCAGCGCTG AGGCGGAGCG AAGCAGGACC AGGGAGCCAG TGACAAAACC GGAAATTCCA
    GTAACATCAG CCTCTGACAA GCCCGCGTTG TCAGAGGAAG AGATGGAGAG GAAGTCCAAA
    TCTATCATTG ATGAGTTTCT ACACATTAAT GATTTTAAGG AAGCCATGCA GTGTGTGGAA
    GAGCTGAACG CCCAGGGCCT GCAACATGTG TTCGTGAGGA CGGGAGTGGA GTCCACCCTG
    GAAAGGAGCC AGATCACCCG GGATCACATG GGCCAGTTAC TGTATCAGCT GGTGCAGTCA
    GAAAAGCTCA GCAAACAGGA CTTTTTCAGA GGTTTTTCAG AAACATTGGA ATTGGCAGAT
    GACATGGCCA TTGATATTCC CCATATTTGG TTGTACCTTG CTGAACTGGT GACTCCCATG
    TTAAAAGAAG GCGGAATCTC CATGAGACAA CTCATCACAG AATTTTGCAA ACCTTTACTT
    CCTGTTGGAA GAGCTGGAAT CTTGATTTCT GAAATATTGC ACCTTCTATG CAAACAAATG
    AGCCATAAGA AAGTGGGAGC CCTATGGAGG GAGGCTGACC TCAGCTGGAA GGACTTTTTA
    CCAGAAGGGG AAGATGTACA TAATTTTCTC TTGGAGCAGA AGTTGGACTT TTTAGAGTCT
    GACAGTTCCT GTTCCTCCGA AGCACTTTTG AAGAAAGAAC TCTCTGCCGA AGAGCTGTAT
    AAGCGACTCG AGAAACTCAT TATTGAGGAC AAGGCAAATG ATGAACAGAT CTTTGACTGG
    GTAGAGGCTA ATCTAGATGA AAGCCAGATG AGTTCACCTA CATTCCTTAG AGCTTTAATG
    ACAGCCGTCT GTAAAGCAGC TATTATAGCC GACTGTTCGA CCTTCCGAGT GGACACTGCT
    GTTATCAAGC AGAGAGTGCC GATCTTACTC AAGTACCTAG ACTCAGATAC AGAGAAGGAA
    CTGCAAGCAC TTTATGCACT ACAAGCATCA ATAGTAAAAC TTGATCAACC TGCCAATTTG
    CTACGGATGT TTTTTGATTG CCTGTATGAC GAGGAGGTGA TCTCTGAGGA TGCCTTCTAC
    AAGTGGGAGA GCAGCAAGGA CCCGGCAGAG CAGAATGGGA AGGGTGTGGC CCTGAAGTCT
    GTCACGGCGT TCTTCACATG GCTGCGGGAA GCAGAAGAGG AGTCTGAGGA TAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317958.1
    CDS162..5198
    Translation

    Target ORF information:

    RefSeq Version XM_015462472.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462472.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCTGCCC
    AGCCAGGTCC CCGAGCACAG CCCTGTGGTC TATGGGACTG TGGAGAGCGC TCATCTTGCT
    GCCAGCACCC CTGTCACTGC AGCGAGCGAC CAGAAGCAAG AAGAGAAGCC AAAACCAGAT
    CCAGTGTTAA AGACTCCTTC CCCAGTCCTT AGGCTAGCCC TCAGTGGAGA GAAGAAAGAA
    CAAGCAGGCC CGACGTCTGA GATTACTGCA GTAGAGTCCA TACCAGAGCT TCCTCTGCCT
    CCATCCCCTA CTGCTATTTC TCCACTTGCT CGAAGTACAA TTACTTCTCC CACCTCTGTT
    GCTCTTAGTA GCCAACCAAT ATTCACCACT GCAATTGATG ACAGATGTGA ACTCTCTTCC
    TCAAAAGAAG ATACAATTCC TATTCCCAGC CCCACATCTT GCACAGAAGC ATCAGATCCT
    TTACCAACAG ATGAAATTGA TGATGATATA TGCAAGAAAT CTTGTAGTGT AGCACCAAAT
    GATATTCCAC TGATTTCTAG TACTAACCTA ATTAATGAAA TGAATGGAGT TAATGAAAAA
    TTACCAGCCA CGGAAAGCAT TGTGGAAATA GTAAAACAGG AGGTGTTACC GCTGACTCTT
    GAATTGGAGA TTTTGGAAAA TCCCCCTGAA GAAATCAAAG TGGAGTGTGT CCCAACTCCC
    ATCACCCCTT CGATAGTTCC CTCCTCTTCT CCGTCTCCTC CAGCTCCTCC AGCTTCTCCT
    TCTCCCACAC CAGTTATCGT TCCTGCTGCT GCCACCAGTG TTAATACTGG TCGTGGTTCC
    ACCGGAGTCC AGAGAGCCTT CGAAGAGGGC GAGAACATAA GAACTTGCCT TAGTGAAGAT
    GCAAAAGAGA TTCAGAACAA AGTAGAGGTA GAAGCAGATG GGCAAACAGA AGAGATTGTG
    GATTCTCAGA ACTTAAGTTC AAGAAAGAGC CCAGTCCCAG CTCAGACAGC TATACCTGCA
    CCAAAGATGT GGAAGAAACC AAAAGACCGG ACCCGAGCCA CTGAAGAGTC GTTAGAGGCA
    GAATCAGAGC CTAAAGCTGA AGAGGAGCTT TCAGGTGACA AAATACTTGA ATCTGAACAG
    GATAAAATGA GCCAAGGGTT TCATCCTGAA AGAGACCCCT CTGACCTAAA AAACGTGAAA
    ACTGTGGAAG AAAATGGAGA AGAAGCTGAG CCTGTACGTA ATGGTGCTGA GAGTATTTCT
    GAGGGTGAAG GAATAGATGC TAATTCAGGC TCCACTGATA GTTCTGGTGA AGGGGTCACA
    TTCCCATTTA AGTCAGAATC CTGGAAGCCT CCTGATTCGG AAGGCAAGAA GCAGTATGAC
    AGGGAGTTTT TGCTGGATTT CCAATTCATG CCAGCGTGTA TTCAGAAACC AGAGGGCCTG
    CCTCCCATCA GTGATGTGGT TCTTGACAAG ATCAATCAAC CAAAATTGCC AATGCGAACT
    CTGGATCCTC GGATTTTGCC TCGAGGACCA GACTTTACTC CAGCCTTTGC AGATTTTGGA
    AGGCAAGCCC CTGGTGGAAG AGGTGTACCT GTTTGCAAAG TGCAGAGCAG GCATGGATTG
    CCAATTCTGG AACAGAGCAA AGCCCCAGCT TCCACTCCAC TGGCGATGTC GCACCCACCC
    CTGAAGAGCC TGCCTCTAGG GCTGTTGAAT GTTGGAGCAC GGAGATCTCA GCCTGGCCAG
    AGGAGAGAGC CCAGGAAGAT CATCACGGTC TCCGTGAAAG AAGACGTGCA CCTGAAGAAG
    GCAGAGAACG CCTGGAAGCC CAGCCAGAGG CGGGATAGCC CAGCCGAGGA CCCCGAGAAC
    ATCAAGACCC AGGAACTTTT TAGAAAAGTT CGAAGTATCT TAAATAAATT GACACCACAG
    ATGTTCAACC AACTGATGAA GCAAGTGTCA GGACTTACTG TTGACACAGA GGAGCGGCTG
    AAAGGAGTCA TTGACCTGGT CTTTGAGAAG GCTATTGACG AACCCAGTTT CTCTGTGGCT
    TACGCAAACA TGTGTCGATG TCTAGTAACG CTGAAAGTAC CCATGGCAGA CAAGCCTGGT
    AACACAGTGA ACTTCCGGAA GCTACTACTG AACCGTTGCC AGAAGGAATT TGAAAAAGAT
    AAAGCCGATG ATGATGTCTT TGAGAAGAAG CAGAAAGAAC TCGAGGCTGC CAGTGCTCCA
    GAGGAGAGGA CAAGGCTTCA TGATGAACTG GAAGAAGCTA AAGATAAAGC CCGGCGGAGA
    TCCATTGGCA ACATCAAGTT CATTGGAGAA CTCTTTAAAC TCAAAATGCT GACTGAAGCC
    ATCATGCATG ACTGTGTGGT GAAGCTGCTG AAGAACCACG ATGAAGAATC CCTCGAGTGT
    CTGTGTCGCC TGCTCACCAC AATTGGCAAA GACCTGGACT TTGAGAAAGC AAAGCCACGT
    ATGGACCAGT ATTTTAATCA GATGGAGAAA ATTGTAAAAG AAAGAAAAAC CTCATCTAGG
    ATTCGATTCA TGCTTCAGGA CGTAATAGAT CTAAGGCTGT GTAATTGGGT ATCACGACGA
    GCAGATCAGG GGCCTAAAAC TATTGAACAG ATTCACAAAG AAGCTAAAAT AGAAGAACAG
    GAAGAGCAGA GGAAGGTCCA ACAACTCATG ACCAAAGAGA AGAGAAGACC AGGTGTCCAG
    AGAGTGGATG AAGGTGGGTG GAACACTGTT CAAGGGGCCA AGAATAGTCG GGTATTGGAC
    CCCTCAAAAT TTCTGAAAAT CACTAAGCCC ACAATTGATG AGAAAATTCA GCTGGTACCT
    AAAGCGCAGC TGGGCAGCTG GGGAAAGGGC AGCAGTGGTG GAGCCAAGGC GAGCGAGGCC
    GATGCCTTAA GATCAAGTGC TTCCAGTTTA AACAGATTCT CTGCTTTGCA ACCTCCAGCA
    CCTTCAGGGT CTGCGTCATC CACACCTCTA GAGTTTGATT CCCGGCGGAC CTTAACTAGT
    CGAGGAAGCA CGGGCAGGGA GAAGAATGAC AAGCCCCTCC AGCCTGCAGC AGCTCGGCCG
    AACACTTTCA TGAGGGGTGG CAGCAGTAAA GACCTGTTAG ACAATCAGTC TCAGGAGGAG
    CAGCGGAGGG AGATGCTGGA GACCGTGAAG CAGCTCACAG GAGGCATGGA CGTGGAGAGG
    AGCAGCGCTG AGGCGGAGCG AAGCAGGACC AGGGAGCCAG TGACAAAACC GGAAATTCCA
    GTAACATCAG CCTCTGACAA GCCCGCGTTG TCAGAGGAAG AGATGGAGAG GAAGTCCAAA
    TCTATCATTG ATGAGTTTCT ACACATTAAT GATTTTAAGG AAGCCATGCA GTGTGTGGAA
    GAGCTGAACG CCCAGGGCCT GCAACATGTG TTCGTGAGGA CGGGAGTGGA GTCCACCCTG
    GAAAGGAGCC AGATCACCCG GGATCACATG GGCCAGTTAC TGTATCAGCT GGTGCAGTCA
    GAAAAGCTCA GCAAACAGGA CTTTTTCAGA GGTTTTTCAG AAACATTGGA ATTGGCAGAT
    GACATGGCCA TTGATATTCC CCATATTTGG TTGTACCTTG CTGAACTGGT GACTCCCATG
    TTAAAAGAAG GCGGAATCTC CATGAGACAA CTCATCACAG AATTTTGCAA ACCTTTACTT
    CCTGTTGGAA GAGCTGGAAT CTTGATTTCT GAAATATTGC ACCTTCTATG CAAACAAATG
    AGCCATAAGA AAGTGGGAGC CCTATGGAGG GAGGCTGACC TCAGCTGGAA GGACTTTTTA
    CCAGAAGGGG AAGATGTACA TAATTTTCTC TTGGAGCAGA AGTTGGACTT TTTAGAGTCT
    GACAGTTCCT GTTCCTCCGA AGCACTTTTG AAGAAAGAAC TCTCTGCCGA AGAGCTGTAT
    AAGCGACTCG AGAAACTCAT TATTGAGGAC AAGGCAAATG ATGAACAGAT CTTTGACTGG
    GTAGAGGCTA ATCTAGATGA AAGCCAGATG AGTTCACCTA CATTCCTTAG AGCTTTAATG
    ACAGCCGTCT GTAAAGCAGC TATTATAGCC GACTGTTCGA CCTTCCGAGT GGACACTGCT
    GTTATCAAGC AGAGAGTGCC GATCTTACTC AAGTACCTAG ACTCAGATAC AGAGAAGGAA
    CTGCAAGCAC TTTATGCACT ACAAGCATCA ATAGTAAAAC TTGATCAACC TGCCAATTTG
    CTACGGATGT TTTTTGATTG CCTGTATGAC GAGGAGGTGA TCTCTGAGGA TGCCTTCTAC
    AAGTGGGAGA GCAGCAAGGA CCCGGCAGAG CAGAATGGGA AGGGTGTGGC CCTGAAGTCT
    GTCACGGCGT TCTTCACATG GCTGCGGGAA GCAGAAGAGG AGTCTGAGGA TAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88090
    Clone ID Related Accession (Same CDS sequence) XM_015460871.2 , XM_015462473.1 , XM_015460871.1
    Accession Version XM_015460871.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460871.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGTTT
    TTCCAGAGGC CTCAAATACA GCCTCCCAGA GCCACCATCC CGAACAGCAG CCCTTCCATT
    CGACCCGGTG CACAGACACC CACTGCAGTG TATCAGGCCA ATCAGCACAT CATGATGGTT
    AACCATCTGC CCATGCCGTA CCCAGTGCCC CAGGGTCCTC AGTACTGTAT ACCACAGTAC
    CGTCACAGTG GCCCTCCGTA TGTTGGGCCA CCACAACAGT ACCCGGTTCA ACCACCAGGA
    CCAGGTCCTT TTTATCCTGG GCCAGGACCT GGGGACTTCC CCAGTGCTTA CGGAACGCCT
    TTTTACCCAA GTCAGCCAGT GTACCAGTCA GCACCTATCA TAGTGCCTAC GCAGCAGCAG
    CCCCCTCCAG CCAAGAGAGA GAAAAAAACA ATAAGAATTC GGGATCCAAA CCAGGGAGGT
    AAAGACATAA CAGAGGAGAT TATGTCTGGA GGTGGCAGCC GAAATCCTAC TCCACCCATA
    GGGAGACCCA CTTCCACACC TACTCCTCCT CAGCAGCTGC CCAGCCAGGT CCCCGAGCAC
    AGCCCTGTGG TCTATGGGAC TGTGGAGAGC GCTCATCTTG CTGCCAGCAC CCCTGTCACT
    GCAGCGAGCG ACCAGAAGCA AGAAGAGAAG CCAAAACCAG ATCCAGTGTT AAAGACTCCT
    TCCCCAGTCC TTAGGCTAGC CCTCAGTGGA GAGAAGAAAG AACAAGCAGG CCCGACGTCT
    GAGATTACTG CAGTAGAGTC CATACCAGAG CTTCCTCTGC CTCCATCCCC TACTGCTATT
    TCTCCACTTG CTCGAAGTAC AATTACTTCT CCCACCTCTG TTGCTCTTAG TAGCCAACCA
    ATATTCACCA CTGCAATTGA TGACAGATGT GAACTCTCTT CCTCAAAAGA AGATACAATT
    CCTATTCCCA GCCCCACATC TTGCACAGAA GCATCAGATC CTTTACCAAC AGATGAAATT
    GATGATGATA TATGCAAGAA ATCTTGTAGT GTAGCACCAA ATGATATTCC ACTGATTTCT
    AGTACTAACC TAATTAATGA AATGAATGGA GTTAATGAAA AATTACCAGC CACGGAAAGC
    ATTGTGGAAA TAGTAAAACA GGAGGTGTTA CCGCTGACTC TTGAATTGGA GATTTTGGAA
    AATCCCCCTG AAGAAATCAA AGTGGAGTGT GTCCCAACTC CCATCACCCC TTCGATAGTT
    CCCTCCTCTT CTCCGTCTCC TCCAGCTCCT CCAGCTTCTC CTTCTCCCAC ACCAGTTATC
    GTTCCTGCTG CTGCCACCAG TGTTAATACT GGTCGTGGTT CCACCGGAGT CCAGAGAGCC
    TTCGAAGAGG GCGAGAACAT AAGAACTTGC CTTAGTGAAG ATGCAAAAGA GATTCAGAAC
    AAAGTAGAGG TAGAAGCAGA TGGGCAAACA GAAGAGATTG TGGATTCTCA GAACTTAAGT
    TCAAGAAAGA GCCCAGTCCC AGCTCAGACA GCTATACCTG CACCAAAGAT GTGGAAGAAA
    CCAAAAGACC GGACCCGAGC CACTGAAGAG TCGTTAGAGG CAGAATCAGA GCCTAAAGCT
    GAAGAGGAGC TTTCAGGTGA CAAAATACTT GAATCTGAAC AGGATAAAAT GAGCCAAGGG
    TTTCATCCTG AAAGAGACCC CTCTGACCTA AAAAACGTGA AAACTGTGGA AGAAAATGGA
    GAAGAAGCTG AGCCTGTACG TAATGGTGCT GAGAGTATTT CTGAGGGTGA AGGAATAGAT
    GCTAATTCAG GCTCCACTGA TAGTTCTGGT GAAGGGGTCA CATTCCCATT TAAGTCAGAA
    TCCTGGAAGC CTCCTGATTC GGAAGGCAAG AAGCAGTATG ACAGGGAGTT TTTGCTGGAT
    TTCCAATTCA TGCCAGCGTG TATTCAGAAA CCAGAGGGCC TGCCTCCCAT CAGTGATGTG
    GTTCTTGACA AGATCAATCA ACCAAAATTG CCAATGCGAA CTCTGGATCC TCGGATTTTG
    CCTCGAGGAC CAGACTTTAC TCCAGCCTTT GCAGATTTTG GAAGGCAAGC CCCTGGTGGA
    AGAGGTGTAC CTGTTTGCAA AGTGCAGAGC AGGCATGGAT TGCCAATTCT GGAACAGAGC
    AAAGCCCCAG CTTCCACTCC ACTGGCGATG TCGCACCCAC CCCTGAAGAG CCTGCCTCTA
    GGGCTGTTGA ATGTTGGAGC ACGGAGATCT CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG
    ATCATCACGG TCTCCGTGAA AGAAGACGTG CACCTGAAGA AGGCAGAGAA CGCCTGGAAG
    CCCAGCCAGA GGCGGGATAG CCCAGCCGAG GACCCCGAGA ACATCAAGAC CCAGGAACTT
    TTTAGAAAAG TTCGAAGTAT CTTAAATAAA TTGACACCAC AGATGTTCAA CCAACTGATG
    AAGCAAGTGT CAGGACTTAC TGTTGACACA GAGGAGCGGC TGAAAGGAGT CATTGACCTG
    GTCTTTGAGA AGGCTATTGA CGAACCCAGT TTCTCTGTGG CTTACGCAAA CATGTGTCGA
    TGTCTAGTAA CGCTGAAAGT ACCCATGGCA GACAAGCCTG GTAACACAGT GAACTTCCGG
    AAGCTACTAC TGAACCGTTG CCAGAAGGAA TTTGAAAAAG ATAAAGCCGA TGATGATGTC
    TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT GCCAGTGCTC CAGAGGAGAG GACAAGGCTT
    CATGATGAAC TGGAAGAAGC TAAAGATAAA GCCCGGCGGA GATCCATTGG CAACATCAAG
    TTCATTGGAG AACTCTTTAA ACTCAAAATG CTGACTGAAG CCATCATGCA TGACTGTGTG
    GTGAAGCTGC TGAAGAACCA CGATGAAGAA TCCCTCGAGT GTCTGTGTCG CCTGCTCACC
    ACAATTGGCA AAGACCTGGA CTTTGAGAAA GCAAAGCCAC GTATGGACCA GTATTTTAAT
    CAGATGGAGA AAATTGTAAA AGAAAGAAAA ACCTCATCTA GGATTCGATT CATGCTTCAG
    GACGTAATAG ATCTAAGGCT GTGTAATTGG GTATCACGAC GAGCAGATCA GGGGCCTAAA
    ACTATTGAAC AGATTCACAA AGAAGCTAAA ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC
    CAACAACTCA TGACCAAAGA GAAGAGAAGA CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG
    TGGAACACTG TTCAAGGGGC CAAGAATAGT CGGGTATTGG ACCCCTCAAA ATTTCTGAAA
    ATCACTAAGC CCACAATTGA TGAGAAAATT CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC
    TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG GCGAGCGAGG CCGATGCCTT AAGATCAAGT
    GCTTCCAGTT TAAACAGATT CTCTGCTTTG CAACCTCCAG CACCTTCAGG GTCTGCGTCA
    TCCACACCTC TAGAGTTTGA TTCCCGGCGG ACCTTAACTA GTCGAGGAAG CACGGGCAGG
    GAGAAGAATG ACAAGCCCCT CCAGCCTGCA GCAGCTCGGC CGAACACTTT CATGAGGGGT
    GGCAGCAGTA AAGACCTGTT AGACAATCAG TCTCAGGAGG AGCAGCGGAG GGAGATGCTG
    GAGACCGTGA AGCAGCTCAC AGGAGGCATG GACGTGGAGA GGAGCAGCGC TGAGGCGGAG
    CGAAGCAGGA CCAGGGAGCC AGTGACAAAA CCGGAAATTC CAGTAACATC AGCCTCTGAC
    AAGCCCGCGT TGTCAGAGGA AGAGATGGAG AGGAAGTCCA AATCTATCAT TGATGAGTTT
    CTACACATTA ATGATTTTAA GGAAGCCATG CAGTGTGTGG AAGAGCTGAA CGCCCAGGGC
    CTGCAACATG TGTTCGTGAG GACGGGAGTG GAGTCCACCC TGGAAAGGAG CCAGATCACC
    CGGGATCACA TGGGCCAGTT ACTGTATCAG CTGGTGCAGT CAGAAAAGCT CAGCAAACAG
    GACTTTTTCA GAGGTTTTTC AGAAACATTG GAATTGGCAG ATGACATGGC CATTGATATT
    CCCCATATTT GGTTGTACCT TGCTGAACTG GTGACTCCCA TGTTAAAAGA AGGCGGAATC
    TCCATGAGAC AACTCATCAC AGAATTTTGC AAACCTTTAC TTCCTGTTGG AAGAGCTGGA
    ATCTTGATTT CTGAAATATT GCACCTTCTA TGCAAACAAA TGAGCCATAA GAAAGTGGGA
    GCCCTATGGA GGGAGGCTGA CCTCAGCTGG AAGGACTTTT TACCAGAAGG GGAAGATGTA
    CATAATTTTC TCTTGGAGCA GAAGTTGGAC TTTTTAGAGT CTGACAGTTC CTGTTCCTCC
    GAAGCACTTT TGAAGAAAGA ACTCTCTGCC GAAGAGCTGT ATAAGCGACT CGAGAAACTC
    ATTATTGAGG ACAAGGCAAA TGATGAACAG ATCTTTGACT GGGTAGAGGC TAATCTAGAT
    GAAAGCCAGA TGAGTTCACC TACATTCCTT AGAGCTTTAA TGACAGCCGT CTGTAAAGCA
    GCTATTATAG CCGACTGTTC GACCTTCCGA GTGGACACTG CTGTTATCAA GCAGAGAGTG
    CCGATCTTAC TCAAGTACCT AGACTCAGAT ACAGAGAAGG AACTGCAAGC ACTTTATGCA
    CTACAAGCAT CAATAGTAAA ACTTGATCAA CCTGCCAATT TGCTACGGAT GTTTTTTGAT
    TGCCTGTATG ACGAGGAGGT GATCTCTGAG GATGCCTTCT ACAAGTGGGA GAGCAGCAAG
    GACCCGGCAG AGCAGAATGG GAAGGGTGTG GCCCTGAAGT CTGTCACGGC GTTCTTCACA
    TGGCTGCGGG AAGCAGAAGA GGAGTCTGAG GATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316357.1
    CDS196..5214
    Translation

    Target ORF information:

    RefSeq Version XM_015460871.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460871.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGTTT
    TTCCAGAGGC CTCAAATACA GCCTCCCAGA GCCACCATCC CGAACAGCAG CCCTTCCATT
    CGACCCGGTG CACAGACACC CACTGCAGTG TATCAGGCCA ATCAGCACAT CATGATGGTT
    AACCATCTGC CCATGCCGTA CCCAGTGCCC CAGGGTCCTC AGTACTGTAT ACCACAGTAC
    CGTCACAGTG GCCCTCCGTA TGTTGGGCCA CCACAACAGT ACCCGGTTCA ACCACCAGGA
    CCAGGTCCTT TTTATCCTGG GCCAGGACCT GGGGACTTCC CCAGTGCTTA CGGAACGCCT
    TTTTACCCAA GTCAGCCAGT GTACCAGTCA GCACCTATCA TAGTGCCTAC GCAGCAGCAG
    CCCCCTCCAG CCAAGAGAGA GAAAAAAACA ATAAGAATTC GGGATCCAAA CCAGGGAGGT
    AAAGACATAA CAGAGGAGAT TATGTCTGGA GGTGGCAGCC GAAATCCTAC TCCACCCATA
    GGGAGACCCA CTTCCACACC TACTCCTCCT CAGCAGCTGC CCAGCCAGGT CCCCGAGCAC
    AGCCCTGTGG TCTATGGGAC TGTGGAGAGC GCTCATCTTG CTGCCAGCAC CCCTGTCACT
    GCAGCGAGCG ACCAGAAGCA AGAAGAGAAG CCAAAACCAG ATCCAGTGTT AAAGACTCCT
    TCCCCAGTCC TTAGGCTAGC CCTCAGTGGA GAGAAGAAAG AACAAGCAGG CCCGACGTCT
    GAGATTACTG CAGTAGAGTC CATACCAGAG CTTCCTCTGC CTCCATCCCC TACTGCTATT
    TCTCCACTTG CTCGAAGTAC AATTACTTCT CCCACCTCTG TTGCTCTTAG TAGCCAACCA
    ATATTCACCA CTGCAATTGA TGACAGATGT GAACTCTCTT CCTCAAAAGA AGATACAATT
    CCTATTCCCA GCCCCACATC TTGCACAGAA GCATCAGATC CTTTACCAAC AGATGAAATT
    GATGATGATA TATGCAAGAA ATCTTGTAGT GTAGCACCAA ATGATATTCC ACTGATTTCT
    AGTACTAACC TAATTAATGA AATGAATGGA GTTAATGAAA AATTACCAGC CACGGAAAGC
    ATTGTGGAAA TAGTAAAACA GGAGGTGTTA CCGCTGACTC TTGAATTGGA GATTTTGGAA
    AATCCCCCTG AAGAAATCAA AGTGGAGTGT GTCCCAACTC CCATCACCCC TTCGATAGTT
    CCCTCCTCTT CTCCGTCTCC TCCAGCTCCT CCAGCTTCTC CTTCTCCCAC ACCAGTTATC
    GTTCCTGCTG CTGCCACCAG TGTTAATACT GGTCGTGGTT CCACCGGAGT CCAGAGAGCC
    TTCGAAGAGG GCGAGAACAT AAGAACTTGC CTTAGTGAAG ATGCAAAAGA GATTCAGAAC
    AAAGTAGAGG TAGAAGCAGA TGGGCAAACA GAAGAGATTG TGGATTCTCA GAACTTAAGT
    TCAAGAAAGA GCCCAGTCCC AGCTCAGACA GCTATACCTG CACCAAAGAT GTGGAAGAAA
    CCAAAAGACC GGACCCGAGC CACTGAAGAG TCGTTAGAGG CAGAATCAGA GCCTAAAGCT
    GAAGAGGAGC TTTCAGGTGA CAAAATACTT GAATCTGAAC AGGATAAAAT GAGCCAAGGG
    TTTCATCCTG AAAGAGACCC CTCTGACCTA AAAAACGTGA AAACTGTGGA AGAAAATGGA
    GAAGAAGCTG AGCCTGTACG TAATGGTGCT GAGAGTATTT CTGAGGGTGA AGGAATAGAT
    GCTAATTCAG GCTCCACTGA TAGTTCTGGT GAAGGGGTCA CATTCCCATT TAAGTCAGAA
    TCCTGGAAGC CTCCTGATTC GGAAGGCAAG AAGCAGTATG ACAGGGAGTT TTTGCTGGAT
    TTCCAATTCA TGCCAGCGTG TATTCAGAAA CCAGAGGGCC TGCCTCCCAT CAGTGATGTG
    GTTCTTGACA AGATCAATCA ACCAAAATTG CCAATGCGAA CTCTGGATCC TCGGATTTTG
    CCTCGAGGAC CAGACTTTAC TCCAGCCTTT GCAGATTTTG GAAGGCAAGC CCCTGGTGGA
    AGAGGTGTAC CTGTTTGCAA AGTGCAGAGC AGGCATGGAT TGCCAATTCT GGAACAGAGC
    AAAGCCCCAG CTTCCACTCC ACTGGCGATG TCGCACCCAC CCCTGAAGAG CCTGCCTCTA
    GGGCTGTTGA ATGTTGGAGC ACGGAGATCT CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG
    ATCATCACGG TCTCCGTGAA AGAAGACGTG CACCTGAAGA AGGCAGAGAA CGCCTGGAAG
    CCCAGCCAGA GGCGGGATAG CCCAGCCGAG GACCCCGAGA ACATCAAGAC CCAGGAACTT
    TTTAGAAAAG TTCGAAGTAT CTTAAATAAA TTGACACCAC AGATGTTCAA CCAACTGATG
    AAGCAAGTGT CAGGACTTAC TGTTGACACA GAGGAGCGGC TGAAAGGAGT CATTGACCTG
    GTCTTTGAGA AGGCTATTGA CGAACCCAGT TTCTCTGTGG CTTACGCAAA CATGTGTCGA
    TGTCTAGTAA CGCTGAAAGT ACCCATGGCA GACAAGCCTG GTAACACAGT GAACTTCCGG
    AAGCTACTAC TGAACCGTTG CCAGAAGGAA TTTGAAAAAG ATAAAGCCGA TGATGATGTC
    TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT GCCAGTGCTC CAGAGGAGAG GACAAGGCTT
    CATGATGAAC TGGAAGAAGC TAAAGATAAA GCCCGGCGGA GATCCATTGG CAACATCAAG
    TTCATTGGAG AACTCTTTAA ACTCAAAATG CTGACTGAAG CCATCATGCA TGACTGTGTG
    GTGAAGCTGC TGAAGAACCA CGATGAAGAA TCCCTCGAGT GTCTGTGTCG CCTGCTCACC
    ACAATTGGCA AAGACCTGGA CTTTGAGAAA GCAAAGCCAC GTATGGACCA GTATTTTAAT
    CAGATGGAGA AAATTGTAAA AGAAAGAAAA ACCTCATCTA GGATTCGATT CATGCTTCAG
    GACGTAATAG ATCTAAGGCT GTGTAATTGG GTATCACGAC GAGCAGATCA GGGGCCTAAA
    ACTATTGAAC AGATTCACAA AGAAGCTAAA ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC
    CAACAACTCA TGACCAAAGA GAAGAGAAGA CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG
    TGGAACACTG TTCAAGGGGC CAAGAATAGT CGGGTATTGG ACCCCTCAAA ATTTCTGAAA
    ATCACTAAGC CCACAATTGA TGAGAAAATT CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC
    TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG GCGAGCGAGG CCGATGCCTT AAGATCAAGT
    GCTTCCAGTT TAAACAGATT CTCTGCTTTG CAACCTCCAG CACCTTCAGG GTCTGCGTCA
    TCCACACCTC TAGAGTTTGA TTCCCGGCGG ACCTTAACTA GTCGAGGAAG CACGGGCAGG
    GAGAAGAATG ACAAGCCCCT CCAGCCTGCA GCAGCTCGGC CGAACACTTT CATGAGGGGT
    GGCAGCAGTA AAGACCTGTT AGACAATCAG TCTCAGGAGG AGCAGCGGAG GGAGATGCTG
    GAGACCGTGA AGCAGCTCAC AGGAGGCATG GACGTGGAGA GGAGCAGCGC TGAGGCGGAG
    CGAAGCAGGA CCAGGGAGCC AGTGACAAAA CCGGAAATTC CAGTAACATC AGCCTCTGAC
    AAGCCCGCGT TGTCAGAGGA AGAGATGGAG AGGAAGTCCA AATCTATCAT TGATGAGTTT
    CTACACATTA ATGATTTTAA GGAAGCCATG CAGTGTGTGG AAGAGCTGAA CGCCCAGGGC
    CTGCAACATG TGTTCGTGAG GACGGGAGTG GAGTCCACCC TGGAAAGGAG CCAGATCACC
    CGGGATCACA TGGGCCAGTT ACTGTATCAG CTGGTGCAGT CAGAAAAGCT CAGCAAACAG
    GACTTTTTCA GAGGTTTTTC AGAAACATTG GAATTGGCAG ATGACATGGC CATTGATATT
    CCCCATATTT GGTTGTACCT TGCTGAACTG GTGACTCCCA TGTTAAAAGA AGGCGGAATC
    TCCATGAGAC AACTCATCAC AGAATTTTGC AAACCTTTAC TTCCTGTTGG AAGAGCTGGA
    ATCTTGATTT CTGAAATATT GCACCTTCTA TGCAAACAAA TGAGCCATAA GAAAGTGGGA
    GCCCTATGGA GGGAGGCTGA CCTCAGCTGG AAGGACTTTT TACCAGAAGG GGAAGATGTA
    CATAATTTTC TCTTGGAGCA GAAGTTGGAC TTTTTAGAGT CTGACAGTTC CTGTTCCTCC
    GAAGCACTTT TGAAGAAAGA ACTCTCTGCC GAAGAGCTGT ATAAGCGACT CGAGAAACTC
    ATTATTGAGG ACAAGGCAAA TGATGAACAG ATCTTTGACT GGGTAGAGGC TAATCTAGAT
    GAAAGCCAGA TGAGTTCACC TACATTCCTT AGAGCTTTAA TGACAGCCGT CTGTAAAGCA
    GCTATTATAG CCGACTGTTC GACCTTCCGA GTGGACACTG CTGTTATCAA GCAGAGAGTG
    CCGATCTTAC TCAAGTACCT AGACTCAGAT ACAGAGAAGG AACTGCAAGC ACTTTATGCA
    CTACAAGCAT CAATAGTAAA ACTTGATCAA CCTGCCAATT TGCTACGGAT GTTTTTTGAT
    TGCCTGTATG ACGAGGAGGT GATCTCTGAG GATGCCTTCT ACAAGTGGGA GAGCAGCAAG
    GACCCGGCAG AGCAGAATGG GAAGGGTGTG GCCCTGAAGT CTGTCACGGC GTTCTTCACA
    TGGCTGCGGG AAGCAGAAGA GGAGTCTGAG GATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88090
    Clone ID Related Accession (Same CDS sequence) XM_015460871.2 , XM_015462473.1 , XM_015460871.1
    Accession Version XM_015462473.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGTTT
    TTCCAGAGGC CTCAAATACA GCCTCCCAGA GCCACCATCC CGAACAGCAG CCCTTCCATT
    CGACCCGGTG CACAGACACC CACTGCAGTG TATCAGGCCA ATCAGCACAT CATGATGGTT
    AACCATCTGC CCATGCCGTA CCCAGTGCCC CAGGGTCCTC AGTACTGTAT ACCACAGTAC
    CGTCACAGTG GCCCTCCGTA TGTTGGGCCA CCACAACAGT ACCCGGTTCA ACCACCAGGA
    CCAGGTCCTT TTTATCCTGG GCCAGGACCT GGGGACTTCC CCAGTGCTTA CGGAACGCCT
    TTTTACCCAA GTCAGCCAGT GTACCAGTCA GCACCTATCA TAGTGCCTAC GCAGCAGCAG
    CCCCCTCCAG CCAAGAGAGA GAAAAAAACA ATAAGAATTC GGGATCCAAA CCAGGGAGGT
    AAAGACATAA CAGAGGAGAT TATGTCTGGA GGTGGCAGCC GAAATCCTAC TCCACCCATA
    GGGAGACCCA CTTCCACACC TACTCCTCCT CAGCAGCTGC CCAGCCAGGT CCCCGAGCAC
    AGCCCTGTGG TCTATGGGAC TGTGGAGAGC GCTCATCTTG CTGCCAGCAC CCCTGTCACT
    GCAGCGAGCG ACCAGAAGCA AGAAGAGAAG CCAAAACCAG ATCCAGTGTT AAAGACTCCT
    TCCCCAGTCC TTAGGCTAGC CCTCAGTGGA GAGAAGAAAG AACAAGCAGG CCCGACGTCT
    GAGATTACTG CAGTAGAGTC CATACCAGAG CTTCCTCTGC CTCCATCCCC TACTGCTATT
    TCTCCACTTG CTCGAAGTAC AATTACTTCT CCCACCTCTG TTGCTCTTAG TAGCCAACCA
    ATATTCACCA CTGCAATTGA TGACAGATGT GAACTCTCTT CCTCAAAAGA AGATACAATT
    CCTATTCCCA GCCCCACATC TTGCACAGAA GCATCAGATC CTTTACCAAC AGATGAAATT
    GATGATGATA TATGCAAGAA ATCTTGTAGT GTAGCACCAA ATGATATTCC ACTGATTTCT
    AGTACTAACC TAATTAATGA AATGAATGGA GTTAATGAAA AATTACCAGC CACGGAAAGC
    ATTGTGGAAA TAGTAAAACA GGAGGTGTTA CCGCTGACTC TTGAATTGGA GATTTTGGAA
    AATCCCCCTG AAGAAATCAA AGTGGAGTGT GTCCCAACTC CCATCACCCC TTCGATAGTT
    CCCTCCTCTT CTCCGTCTCC TCCAGCTCCT CCAGCTTCTC CTTCTCCCAC ACCAGTTATC
    GTTCCTGCTG CTGCCACCAG TGTTAATACT GGTCGTGGTT CCACCGGAGT CCAGAGAGCC
    TTCGAAGAGG GCGAGAACAT AAGAACTTGC CTTAGTGAAG ATGCAAAAGA GATTCAGAAC
    AAAGTAGAGG TAGAAGCAGA TGGGCAAACA GAAGAGATTG TGGATTCTCA GAACTTAAGT
    TCAAGAAAGA GCCCAGTCCC AGCTCAGACA GCTATACCTG CACCAAAGAT GTGGAAGAAA
    CCAAAAGACC GGACCCGAGC CACTGAAGAG TCGTTAGAGG CAGAATCAGA GCCTAAAGCT
    GAAGAGGAGC TTTCAGGTGA CAAAATACTT GAATCTGAAC AGGATAAAAT GAGCCAAGGG
    TTTCATCCTG AAAGAGACCC CTCTGACCTA AAAAACGTGA AAACTGTGGA AGAAAATGGA
    GAAGAAGCTG AGCCTGTACG TAATGGTGCT GAGAGTATTT CTGAGGGTGA AGGAATAGAT
    GCTAATTCAG GCTCCACTGA TAGTTCTGGT GAAGGGGTCA CATTCCCATT TAAGTCAGAA
    TCCTGGAAGC CTCCTGATTC GGAAGGCAAG AAGCAGTATG ACAGGGAGTT TTTGCTGGAT
    TTCCAATTCA TGCCAGCGTG TATTCAGAAA CCAGAGGGCC TGCCTCCCAT CAGTGATGTG
    GTTCTTGACA AGATCAATCA ACCAAAATTG CCAATGCGAA CTCTGGATCC TCGGATTTTG
    CCTCGAGGAC CAGACTTTAC TCCAGCCTTT GCAGATTTTG GAAGGCAAGC CCCTGGTGGA
    AGAGGTGTAC CTGTTTGCAA AGTGCAGAGC AGGCATGGAT TGCCAATTCT GGAACAGAGC
    AAAGCCCCAG CTTCCACTCC ACTGGCGATG TCGCACCCAC CCCTGAAGAG CCTGCCTCTA
    GGGCTGTTGA ATGTTGGAGC ACGGAGATCT CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG
    ATCATCACGG TCTCCGTGAA AGAAGACGTG CACCTGAAGA AGGCAGAGAA CGCCTGGAAG
    CCCAGCCAGA GGCGGGATAG CCCAGCCGAG GACCCCGAGA ACATCAAGAC CCAGGAACTT
    TTTAGAAAAG TTCGAAGTAT CTTAAATAAA TTGACACCAC AGATGTTCAA CCAACTGATG
    AAGCAAGTGT CAGGACTTAC TGTTGACACA GAGGAGCGGC TGAAAGGAGT CATTGACCTG
    GTCTTTGAGA AGGCTATTGA CGAACCCAGT TTCTCTGTGG CTTACGCAAA CATGTGTCGA
    TGTCTAGTAA CGCTGAAAGT ACCCATGGCA GACAAGCCTG GTAACACAGT GAACTTCCGG
    AAGCTACTAC TGAACCGTTG CCAGAAGGAA TTTGAAAAAG ATAAAGCCGA TGATGATGTC
    TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT GCCAGTGCTC CAGAGGAGAG GACAAGGCTT
    CATGATGAAC TGGAAGAAGC TAAAGATAAA GCCCGGCGGA GATCCATTGG CAACATCAAG
    TTCATTGGAG AACTCTTTAA ACTCAAAATG CTGACTGAAG CCATCATGCA TGACTGTGTG
    GTGAAGCTGC TGAAGAACCA CGATGAAGAA TCCCTCGAGT GTCTGTGTCG CCTGCTCACC
    ACAATTGGCA AAGACCTGGA CTTTGAGAAA GCAAAGCCAC GTATGGACCA GTATTTTAAT
    CAGATGGAGA AAATTGTAAA AGAAAGAAAA ACCTCATCTA GGATTCGATT CATGCTTCAG
    GACGTAATAG ATCTAAGGCT GTGTAATTGG GTATCACGAC GAGCAGATCA GGGGCCTAAA
    ACTATTGAAC AGATTCACAA AGAAGCTAAA ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC
    CAACAACTCA TGACCAAAGA GAAGAGAAGA CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG
    TGGAACACTG TTCAAGGGGC CAAGAATAGT CGGGTATTGG ACCCCTCAAA ATTTCTGAAA
    ATCACTAAGC CCACAATTGA TGAGAAAATT CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC
    TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG GCGAGCGAGG CCGATGCCTT AAGATCAAGT
    GCTTCCAGTT TAAACAGATT CTCTGCTTTG CAACCTCCAG CACCTTCAGG GTCTGCGTCA
    TCCACACCTC TAGAGTTTGA TTCCCGGCGG ACCTTAACTA GTCGAGGAAG CACGGGCAGG
    GAGAAGAATG ACAAGCCCCT CCAGCCTGCA GCAGCTCGGC CGAACACTTT CATGAGGGGT
    GGCAGCAGTA AAGACCTGTT AGACAATCAG TCTCAGGAGG AGCAGCGGAG GGAGATGCTG
    GAGACCGTGA AGCAGCTCAC AGGAGGCATG GACGTGGAGA GGAGCAGCGC TGAGGCGGAG
    CGAAGCAGGA CCAGGGAGCC AGTGACAAAA CCGGAAATTC CAGTAACATC AGCCTCTGAC
    AAGCCCGCGT TGTCAGAGGA AGAGATGGAG AGGAAGTCCA AATCTATCAT TGATGAGTTT
    CTACACATTA ATGATTTTAA GGAAGCCATG CAGTGTGTGG AAGAGCTGAA CGCCCAGGGC
    CTGCAACATG TGTTCGTGAG GACGGGAGTG GAGTCCACCC TGGAAAGGAG CCAGATCACC
    CGGGATCACA TGGGCCAGTT ACTGTATCAG CTGGTGCAGT CAGAAAAGCT CAGCAAACAG
    GACTTTTTCA GAGGTTTTTC AGAAACATTG GAATTGGCAG ATGACATGGC CATTGATATT
    CCCCATATTT GGTTGTACCT TGCTGAACTG GTGACTCCCA TGTTAAAAGA AGGCGGAATC
    TCCATGAGAC AACTCATCAC AGAATTTTGC AAACCTTTAC TTCCTGTTGG AAGAGCTGGA
    ATCTTGATTT CTGAAATATT GCACCTTCTA TGCAAACAAA TGAGCCATAA GAAAGTGGGA
    GCCCTATGGA GGGAGGCTGA CCTCAGCTGG AAGGACTTTT TACCAGAAGG GGAAGATGTA
    CATAATTTTC TCTTGGAGCA GAAGTTGGAC TTTTTAGAGT CTGACAGTTC CTGTTCCTCC
    GAAGCACTTT TGAAGAAAGA ACTCTCTGCC GAAGAGCTGT ATAAGCGACT CGAGAAACTC
    ATTATTGAGG ACAAGGCAAA TGATGAACAG ATCTTTGACT GGGTAGAGGC TAATCTAGAT
    GAAAGCCAGA TGAGTTCACC TACATTCCTT AGAGCTTTAA TGACAGCCGT CTGTAAAGCA
    GCTATTATAG CCGACTGTTC GACCTTCCGA GTGGACACTG CTGTTATCAA GCAGAGAGTG
    CCGATCTTAC TCAAGTACCT AGACTCAGAT ACAGAGAAGG AACTGCAAGC ACTTTATGCA
    CTACAAGCAT CAATAGTAAA ACTTGATCAA CCTGCCAATT TGCTACGGAT GTTTTTTGAT
    TGCCTGTATG ACGAGGAGGT GATCTCTGAG GATGCCTTCT ACAAGTGGGA GAGCAGCAAG
    GACCCGGCAG AGCAGAATGG GAAGGGTGTG GCCCTGAAGT CTGTCACGGC GTTCTTCACA
    TGGCTGCGGG AAGCAGAAGA GGAGTCTGAG GATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317959.1
    CDS162..5180
    Translation

    Target ORF information:

    RefSeq Version XM_015462473.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462473.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGTTT
    TTCCAGAGGC CTCAAATACA GCCTCCCAGA GCCACCATCC CGAACAGCAG CCCTTCCATT
    CGACCCGGTG CACAGACACC CACTGCAGTG TATCAGGCCA ATCAGCACAT CATGATGGTT
    AACCATCTGC CCATGCCGTA CCCAGTGCCC CAGGGTCCTC AGTACTGTAT ACCACAGTAC
    CGTCACAGTG GCCCTCCGTA TGTTGGGCCA CCACAACAGT ACCCGGTTCA ACCACCAGGA
    CCAGGTCCTT TTTATCCTGG GCCAGGACCT GGGGACTTCC CCAGTGCTTA CGGAACGCCT
    TTTTACCCAA GTCAGCCAGT GTACCAGTCA GCACCTATCA TAGTGCCTAC GCAGCAGCAG
    CCCCCTCCAG CCAAGAGAGA GAAAAAAACA ATAAGAATTC GGGATCCAAA CCAGGGAGGT
    AAAGACATAA CAGAGGAGAT TATGTCTGGA GGTGGCAGCC GAAATCCTAC TCCACCCATA
    GGGAGACCCA CTTCCACACC TACTCCTCCT CAGCAGCTGC CCAGCCAGGT CCCCGAGCAC
    AGCCCTGTGG TCTATGGGAC TGTGGAGAGC GCTCATCTTG CTGCCAGCAC CCCTGTCACT
    GCAGCGAGCG ACCAGAAGCA AGAAGAGAAG CCAAAACCAG ATCCAGTGTT AAAGACTCCT
    TCCCCAGTCC TTAGGCTAGC CCTCAGTGGA GAGAAGAAAG AACAAGCAGG CCCGACGTCT
    GAGATTACTG CAGTAGAGTC CATACCAGAG CTTCCTCTGC CTCCATCCCC TACTGCTATT
    TCTCCACTTG CTCGAAGTAC AATTACTTCT CCCACCTCTG TTGCTCTTAG TAGCCAACCA
    ATATTCACCA CTGCAATTGA TGACAGATGT GAACTCTCTT CCTCAAAAGA AGATACAATT
    CCTATTCCCA GCCCCACATC TTGCACAGAA GCATCAGATC CTTTACCAAC AGATGAAATT
    GATGATGATA TATGCAAGAA ATCTTGTAGT GTAGCACCAA ATGATATTCC ACTGATTTCT
    AGTACTAACC TAATTAATGA AATGAATGGA GTTAATGAAA AATTACCAGC CACGGAAAGC
    ATTGTGGAAA TAGTAAAACA GGAGGTGTTA CCGCTGACTC TTGAATTGGA GATTTTGGAA
    AATCCCCCTG AAGAAATCAA AGTGGAGTGT GTCCCAACTC CCATCACCCC TTCGATAGTT
    CCCTCCTCTT CTCCGTCTCC TCCAGCTCCT CCAGCTTCTC CTTCTCCCAC ACCAGTTATC
    GTTCCTGCTG CTGCCACCAG TGTTAATACT GGTCGTGGTT CCACCGGAGT CCAGAGAGCC
    TTCGAAGAGG GCGAGAACAT AAGAACTTGC CTTAGTGAAG ATGCAAAAGA GATTCAGAAC
    AAAGTAGAGG TAGAAGCAGA TGGGCAAACA GAAGAGATTG TGGATTCTCA GAACTTAAGT
    TCAAGAAAGA GCCCAGTCCC AGCTCAGACA GCTATACCTG CACCAAAGAT GTGGAAGAAA
    CCAAAAGACC GGACCCGAGC CACTGAAGAG TCGTTAGAGG CAGAATCAGA GCCTAAAGCT
    GAAGAGGAGC TTTCAGGTGA CAAAATACTT GAATCTGAAC AGGATAAAAT GAGCCAAGGG
    TTTCATCCTG AAAGAGACCC CTCTGACCTA AAAAACGTGA AAACTGTGGA AGAAAATGGA
    GAAGAAGCTG AGCCTGTACG TAATGGTGCT GAGAGTATTT CTGAGGGTGA AGGAATAGAT
    GCTAATTCAG GCTCCACTGA TAGTTCTGGT GAAGGGGTCA CATTCCCATT TAAGTCAGAA
    TCCTGGAAGC CTCCTGATTC GGAAGGCAAG AAGCAGTATG ACAGGGAGTT TTTGCTGGAT
    TTCCAATTCA TGCCAGCGTG TATTCAGAAA CCAGAGGGCC TGCCTCCCAT CAGTGATGTG
    GTTCTTGACA AGATCAATCA ACCAAAATTG CCAATGCGAA CTCTGGATCC TCGGATTTTG
    CCTCGAGGAC CAGACTTTAC TCCAGCCTTT GCAGATTTTG GAAGGCAAGC CCCTGGTGGA
    AGAGGTGTAC CTGTTTGCAA AGTGCAGAGC AGGCATGGAT TGCCAATTCT GGAACAGAGC
    AAAGCCCCAG CTTCCACTCC ACTGGCGATG TCGCACCCAC CCCTGAAGAG CCTGCCTCTA
    GGGCTGTTGA ATGTTGGAGC ACGGAGATCT CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG
    ATCATCACGG TCTCCGTGAA AGAAGACGTG CACCTGAAGA AGGCAGAGAA CGCCTGGAAG
    CCCAGCCAGA GGCGGGATAG CCCAGCCGAG GACCCCGAGA ACATCAAGAC CCAGGAACTT
    TTTAGAAAAG TTCGAAGTAT CTTAAATAAA TTGACACCAC AGATGTTCAA CCAACTGATG
    AAGCAAGTGT CAGGACTTAC TGTTGACACA GAGGAGCGGC TGAAAGGAGT CATTGACCTG
    GTCTTTGAGA AGGCTATTGA CGAACCCAGT TTCTCTGTGG CTTACGCAAA CATGTGTCGA
    TGTCTAGTAA CGCTGAAAGT ACCCATGGCA GACAAGCCTG GTAACACAGT GAACTTCCGG
    AAGCTACTAC TGAACCGTTG CCAGAAGGAA TTTGAAAAAG ATAAAGCCGA TGATGATGTC
    TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT GCCAGTGCTC CAGAGGAGAG GACAAGGCTT
    CATGATGAAC TGGAAGAAGC TAAAGATAAA GCCCGGCGGA GATCCATTGG CAACATCAAG
    TTCATTGGAG AACTCTTTAA ACTCAAAATG CTGACTGAAG CCATCATGCA TGACTGTGTG
    GTGAAGCTGC TGAAGAACCA CGATGAAGAA TCCCTCGAGT GTCTGTGTCG CCTGCTCACC
    ACAATTGGCA AAGACCTGGA CTTTGAGAAA GCAAAGCCAC GTATGGACCA GTATTTTAAT
    CAGATGGAGA AAATTGTAAA AGAAAGAAAA ACCTCATCTA GGATTCGATT CATGCTTCAG
    GACGTAATAG ATCTAAGGCT GTGTAATTGG GTATCACGAC GAGCAGATCA GGGGCCTAAA
    ACTATTGAAC AGATTCACAA AGAAGCTAAA ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC
    CAACAACTCA TGACCAAAGA GAAGAGAAGA CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG
    TGGAACACTG TTCAAGGGGC CAAGAATAGT CGGGTATTGG ACCCCTCAAA ATTTCTGAAA
    ATCACTAAGC CCACAATTGA TGAGAAAATT CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC
    TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG GCGAGCGAGG CCGATGCCTT AAGATCAAGT
    GCTTCCAGTT TAAACAGATT CTCTGCTTTG CAACCTCCAG CACCTTCAGG GTCTGCGTCA
    TCCACACCTC TAGAGTTTGA TTCCCGGCGG ACCTTAACTA GTCGAGGAAG CACGGGCAGG
    GAGAAGAATG ACAAGCCCCT CCAGCCTGCA GCAGCTCGGC CGAACACTTT CATGAGGGGT
    GGCAGCAGTA AAGACCTGTT AGACAATCAG TCTCAGGAGG AGCAGCGGAG GGAGATGCTG
    GAGACCGTGA AGCAGCTCAC AGGAGGCATG GACGTGGAGA GGAGCAGCGC TGAGGCGGAG
    CGAAGCAGGA CCAGGGAGCC AGTGACAAAA CCGGAAATTC CAGTAACATC AGCCTCTGAC
    AAGCCCGCGT TGTCAGAGGA AGAGATGGAG AGGAAGTCCA AATCTATCAT TGATGAGTTT
    CTACACATTA ATGATTTTAA GGAAGCCATG CAGTGTGTGG AAGAGCTGAA CGCCCAGGGC
    CTGCAACATG TGTTCGTGAG GACGGGAGTG GAGTCCACCC TGGAAAGGAG CCAGATCACC
    CGGGATCACA TGGGCCAGTT ACTGTATCAG CTGGTGCAGT CAGAAAAGCT CAGCAAACAG
    GACTTTTTCA GAGGTTTTTC AGAAACATTG GAATTGGCAG ATGACATGGC CATTGATATT
    CCCCATATTT GGTTGTACCT TGCTGAACTG GTGACTCCCA TGTTAAAAGA AGGCGGAATC
    TCCATGAGAC AACTCATCAC AGAATTTTGC AAACCTTTAC TTCCTGTTGG AAGAGCTGGA
    ATCTTGATTT CTGAAATATT GCACCTTCTA TGCAAACAAA TGAGCCATAA GAAAGTGGGA
    GCCCTATGGA GGGAGGCTGA CCTCAGCTGG AAGGACTTTT TACCAGAAGG GGAAGATGTA
    CATAATTTTC TCTTGGAGCA GAAGTTGGAC TTTTTAGAGT CTGACAGTTC CTGTTCCTCC
    GAAGCACTTT TGAAGAAAGA ACTCTCTGCC GAAGAGCTGT ATAAGCGACT CGAGAAACTC
    ATTATTGAGG ACAAGGCAAA TGATGAACAG ATCTTTGACT GGGTAGAGGC TAATCTAGAT
    GAAAGCCAGA TGAGTTCACC TACATTCCTT AGAGCTTTAA TGACAGCCGT CTGTAAAGCA
    GCTATTATAG CCGACTGTTC GACCTTCCGA GTGGACACTG CTGTTATCAA GCAGAGAGTG
    CCGATCTTAC TCAAGTACCT AGACTCAGAT ACAGAGAAGG AACTGCAAGC ACTTTATGCA
    CTACAAGCAT CAATAGTAAA ACTTGATCAA CCTGCCAATT TGCTACGGAT GTTTTTTGAT
    TGCCTGTATG ACGAGGAGGT GATCTCTGAG GATGCCTTCT ACAAGTGGGA GAGCAGCAAG
    GACCCGGCAG AGCAGAATGG GAAGGGTGTG GCCCTGAAGT CTGTCACGGC GTTCTTCACA
    TGGCTGCGGG AAGCAGAAGA GGAGTCTGAG GATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88090
    Clone ID Related Accession (Same CDS sequence) XM_015460871.2 , XM_015462473.1 , XM_015460871.1
    Accession Version XM_015460871.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGTTT
    TTCCAGAGGC CTCAAATACA GCCTCCCAGA GCCACCATCC CGAACAGCAG CCCTTCCATT
    CGACCCGGTG CACAGACACC CACTGCAGTG TATCAGGCCA ATCAGCACAT CATGATGGTT
    AACCATCTGC CCATGCCGTA CCCAGTGCCC CAGGGTCCTC AGTACTGTAT ACCACAGTAC
    CGTCACAGTG GCCCTCCGTA TGTTGGGCCA CCACAACAGT ACCCGGTTCA ACCACCAGGA
    CCAGGTCCTT TTTATCCTGG GCCAGGACCT GGGGACTTCC CCAGTGCTTA CGGAACGCCT
    TTTTACCCAA GTCAGCCAGT GTACCAGTCA GCACCTATCA TAGTGCCTAC GCAGCAGCAG
    CCCCCTCCAG CCAAGAGAGA GAAAAAAACA ATAAGAATTC GGGATCCAAA CCAGGGAGGT
    AAAGACATAA CAGAGGAGAT TATGTCTGGA GGTGGCAGCC GAAATCCTAC TCCACCCATA
    GGGAGACCCA CTTCCACACC TACTCCTCCT CAGCAGCTGC CCAGCCAGGT CCCCGAGCAC
    AGCCCTGTGG TCTATGGGAC TGTGGAGAGC GCTCATCTTG CTGCCAGCAC CCCTGTCACT
    GCAGCGAGCG ACCAGAAGCA AGAAGAGAAG CCAAAACCAG ATCCAGTGTT AAAGACTCCT
    TCCCCAGTCC TTAGGCTAGC CCTCAGTGGA GAGAAGAAAG AACAAGCAGG CCCGACGTCT
    GAGATTACTG CAGTAGAGTC CATACCAGAG CTTCCTCTGC CTCCATCCCC TACTGCTATT
    TCTCCACTTG CTCGAAGTAC AATTACTTCT CCCACCTCTG TTGCTCTTAG TAGCCAACCA
    ATATTCACCA CTGCAATTGA TGACAGATGT GAACTCTCTT CCTCAAAAGA AGATACAATT
    CCTATTCCCA GCCCCACATC TTGCACAGAA GCATCAGATC CTTTACCAAC AGATGAAATT
    GATGATGATA TATGCAAGAA ATCTTGTAGT GTAGCACCAA ATGATATTCC ACTGATTTCT
    AGTACTAACC TAATTAATGA AATGAATGGA GTTAATGAAA AATTACCAGC CACGGAAAGC
    ATTGTGGAAA TAGTAAAACA GGAGGTGTTA CCGCTGACTC TTGAATTGGA GATTTTGGAA
    AATCCCCCTG AAGAAATCAA AGTGGAGTGT GTCCCAACTC CCATCACCCC TTCGATAGTT
    CCCTCCTCTT CTCCGTCTCC TCCAGCTCCT CCAGCTTCTC CTTCTCCCAC ACCAGTTATC
    GTTCCTGCTG CTGCCACCAG TGTTAATACT GGTCGTGGTT CCACCGGAGT CCAGAGAGCC
    TTCGAAGAGG GCGAGAACAT AAGAACTTGC CTTAGTGAAG ATGCAAAAGA GATTCAGAAC
    AAAGTAGAGG TAGAAGCAGA TGGGCAAACA GAAGAGATTG TGGATTCTCA GAACTTAAGT
    TCAAGAAAGA GCCCAGTCCC AGCTCAGACA GCTATACCTG CACCAAAGAT GTGGAAGAAA
    CCAAAAGACC GGACCCGAGC CACTGAAGAG TCGTTAGAGG CAGAATCAGA GCCTAAAGCT
    GAAGAGGAGC TTTCAGGTGA CAAAATACTT GAATCTGAAC AGGATAAAAT GAGCCAAGGG
    TTTCATCCTG AAAGAGACCC CTCTGACCTA AAAAACGTGA AAACTGTGGA AGAAAATGGA
    GAAGAAGCTG AGCCTGTACG TAATGGTGCT GAGAGTATTT CTGAGGGTGA AGGAATAGAT
    GCTAATTCAG GCTCCACTGA TAGTTCTGGT GAAGGGGTCA CATTCCCATT TAAGTCAGAA
    TCCTGGAAGC CTCCTGATTC GGAAGGCAAG AAGCAGTATG ACAGGGAGTT TTTGCTGGAT
    TTCCAATTCA TGCCAGCGTG TATTCAGAAA CCAGAGGGCC TGCCTCCCAT CAGTGATGTG
    GTTCTTGACA AGATCAATCA ACCAAAATTG CCAATGCGAA CTCTGGATCC TCGGATTTTG
    CCTCGAGGAC CAGACTTTAC TCCAGCCTTT GCAGATTTTG GAAGGCAAGC CCCTGGTGGA
    AGAGGTGTAC CTGTTTGCAA AGTGCAGAGC AGGCATGGAT TGCCAATTCT GGAACAGAGC
    AAAGCCCCAG CTTCCACTCC ACTGGCGATG TCGCACCCAC CCCTGAAGAG CCTGCCTCTA
    GGGCTGTTGA ATGTTGGAGC ACGGAGATCT CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG
    ATCATCACGG TCTCCGTGAA AGAAGACGTG CACCTGAAGA AGGCAGAGAA CGCCTGGAAG
    CCCAGCCAGA GGCGGGATAG CCCAGCCGAG GACCCCGAGA ACATCAAGAC CCAGGAACTT
    TTTAGAAAAG TTCGAAGTAT CTTAAATAAA TTGACACCAC AGATGTTCAA CCAACTGATG
    AAGCAAGTGT CAGGACTTAC TGTTGACACA GAGGAGCGGC TGAAAGGAGT CATTGACCTG
    GTCTTTGAGA AGGCTATTGA CGAACCCAGT TTCTCTGTGG CTTACGCAAA CATGTGTCGA
    TGTCTAGTAA CGCTGAAAGT ACCCATGGCA GACAAGCCTG GTAACACAGT GAACTTCCGG
    AAGCTACTAC TGAACCGTTG CCAGAAGGAA TTTGAAAAAG ATAAAGCCGA TGATGATGTC
    TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT GCCAGTGCTC CAGAGGAGAG GACAAGGCTT
    CATGATGAAC TGGAAGAAGC TAAAGATAAA GCCCGGCGGA GATCCATTGG CAACATCAAG
    TTCATTGGAG AACTCTTTAA ACTCAAAATG CTGACTGAAG CCATCATGCA TGACTGTGTG
    GTGAAGCTGC TGAAGAACCA CGATGAAGAA TCCCTCGAGT GTCTGTGTCG CCTGCTCACC
    ACAATTGGCA AAGACCTGGA CTTTGAGAAA GCAAAGCCAC GTATGGACCA GTATTTTAAT
    CAGATGGAGA AAATTGTAAA AGAAAGAAAA ACCTCATCTA GGATTCGATT CATGCTTCAG
    GACGTAATAG ATCTAAGGCT GTGTAATTGG GTATCACGAC GAGCAGATCA GGGGCCTAAA
    ACTATTGAAC AGATTCACAA AGAAGCTAAA ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC
    CAACAACTCA TGACCAAAGA GAAGAGAAGA CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG
    TGGAACACTG TTCAAGGGGC CAAGAATAGT CGGGTATTGG ACCCCTCAAA ATTTCTGAAA
    ATCACTAAGC CCACAATTGA TGAGAAAATT CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC
    TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG GCGAGCGAGG CCGATGCCTT AAGATCAAGT
    GCTTCCAGTT TAAACAGATT CTCTGCTTTG CAACCTCCAG CACCTTCAGG GTCTGCGTCA
    TCCACACCTC TAGAGTTTGA TTCCCGGCGG ACCTTAACTA GTCGAGGAAG CACGGGCAGG
    GAGAAGAATG ACAAGCCCCT CCAGCCTGCA GCAGCTCGGC CGAACACTTT CATGAGGGGT
    GGCAGCAGTA AAGACCTGTT AGACAATCAG TCTCAGGAGG AGCAGCGGAG GGAGATGCTG
    GAGACCGTGA AGCAGCTCAC AGGAGGCATG GACGTGGAGA GGAGCAGCGC TGAGGCGGAG
    CGAAGCAGGA CCAGGGAGCC AGTGACAAAA CCGGAAATTC CAGTAACATC AGCCTCTGAC
    AAGCCCGCGT TGTCAGAGGA AGAGATGGAG AGGAAGTCCA AATCTATCAT TGATGAGTTT
    CTACACATTA ATGATTTTAA GGAAGCCATG CAGTGTGTGG AAGAGCTGAA CGCCCAGGGC
    CTGCAACATG TGTTCGTGAG GACGGGAGTG GAGTCCACCC TGGAAAGGAG CCAGATCACC
    CGGGATCACA TGGGCCAGTT ACTGTATCAG CTGGTGCAGT CAGAAAAGCT CAGCAAACAG
    GACTTTTTCA GAGGTTTTTC AGAAACATTG GAATTGGCAG ATGACATGGC CATTGATATT
    CCCCATATTT GGTTGTACCT TGCTGAACTG GTGACTCCCA TGTTAAAAGA AGGCGGAATC
    TCCATGAGAC AACTCATCAC AGAATTTTGC AAACCTTTAC TTCCTGTTGG AAGAGCTGGA
    ATCTTGATTT CTGAAATATT GCACCTTCTA TGCAAACAAA TGAGCCATAA GAAAGTGGGA
    GCCCTATGGA GGGAGGCTGA CCTCAGCTGG AAGGACTTTT TACCAGAAGG GGAAGATGTA
    CATAATTTTC TCTTGGAGCA GAAGTTGGAC TTTTTAGAGT CTGACAGTTC CTGTTCCTCC
    GAAGCACTTT TGAAGAAAGA ACTCTCTGCC GAAGAGCTGT ATAAGCGACT CGAGAAACTC
    ATTATTGAGG ACAAGGCAAA TGATGAACAG ATCTTTGACT GGGTAGAGGC TAATCTAGAT
    GAAAGCCAGA TGAGTTCACC TACATTCCTT AGAGCTTTAA TGACAGCCGT CTGTAAAGCA
    GCTATTATAG CCGACTGTTC GACCTTCCGA GTGGACACTG CTGTTATCAA GCAGAGAGTG
    CCGATCTTAC TCAAGTACCT AGACTCAGAT ACAGAGAAGG AACTGCAAGC ACTTTATGCA
    CTACAAGCAT CAATAGTAAA ACTTGATCAA CCTGCCAATT TGCTACGGAT GTTTTTTGAT
    TGCCTGTATG ACGAGGAGGT GATCTCTGAG GATGCCTTCT ACAAGTGGGA GAGCAGCAAG
    GACCCGGCAG AGCAGAATGG GAAGGGTGTG GCCCTGAAGT CTGTCACGGC GTTCTTCACA
    TGGCTGCGGG AAGCAGAAGA GGAGTCTGAG GATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316357.1
    CDS162..5180
    Translation

    Target ORF information:

    RefSeq Version XM_015460871.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460871.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGTTT
    TTCCAGAGGC CTCAAATACA GCCTCCCAGA GCCACCATCC CGAACAGCAG CCCTTCCATT
    CGACCCGGTG CACAGACACC CACTGCAGTG TATCAGGCCA ATCAGCACAT CATGATGGTT
    AACCATCTGC CCATGCCGTA CCCAGTGCCC CAGGGTCCTC AGTACTGTAT ACCACAGTAC
    CGTCACAGTG GCCCTCCGTA TGTTGGGCCA CCACAACAGT ACCCGGTTCA ACCACCAGGA
    CCAGGTCCTT TTTATCCTGG GCCAGGACCT GGGGACTTCC CCAGTGCTTA CGGAACGCCT
    TTTTACCCAA GTCAGCCAGT GTACCAGTCA GCACCTATCA TAGTGCCTAC GCAGCAGCAG
    CCCCCTCCAG CCAAGAGAGA GAAAAAAACA ATAAGAATTC GGGATCCAAA CCAGGGAGGT
    AAAGACATAA CAGAGGAGAT TATGTCTGGA GGTGGCAGCC GAAATCCTAC TCCACCCATA
    GGGAGACCCA CTTCCACACC TACTCCTCCT CAGCAGCTGC CCAGCCAGGT CCCCGAGCAC
    AGCCCTGTGG TCTATGGGAC TGTGGAGAGC GCTCATCTTG CTGCCAGCAC CCCTGTCACT
    GCAGCGAGCG ACCAGAAGCA AGAAGAGAAG CCAAAACCAG ATCCAGTGTT AAAGACTCCT
    TCCCCAGTCC TTAGGCTAGC CCTCAGTGGA GAGAAGAAAG AACAAGCAGG CCCGACGTCT
    GAGATTACTG CAGTAGAGTC CATACCAGAG CTTCCTCTGC CTCCATCCCC TACTGCTATT
    TCTCCACTTG CTCGAAGTAC AATTACTTCT CCCACCTCTG TTGCTCTTAG TAGCCAACCA
    ATATTCACCA CTGCAATTGA TGACAGATGT GAACTCTCTT CCTCAAAAGA AGATACAATT
    CCTATTCCCA GCCCCACATC TTGCACAGAA GCATCAGATC CTTTACCAAC AGATGAAATT
    GATGATGATA TATGCAAGAA ATCTTGTAGT GTAGCACCAA ATGATATTCC ACTGATTTCT
    AGTACTAACC TAATTAATGA AATGAATGGA GTTAATGAAA AATTACCAGC CACGGAAAGC
    ATTGTGGAAA TAGTAAAACA GGAGGTGTTA CCGCTGACTC TTGAATTGGA GATTTTGGAA
    AATCCCCCTG AAGAAATCAA AGTGGAGTGT GTCCCAACTC CCATCACCCC TTCGATAGTT
    CCCTCCTCTT CTCCGTCTCC TCCAGCTCCT CCAGCTTCTC CTTCTCCCAC ACCAGTTATC
    GTTCCTGCTG CTGCCACCAG TGTTAATACT GGTCGTGGTT CCACCGGAGT CCAGAGAGCC
    TTCGAAGAGG GCGAGAACAT AAGAACTTGC CTTAGTGAAG ATGCAAAAGA GATTCAGAAC
    AAAGTAGAGG TAGAAGCAGA TGGGCAAACA GAAGAGATTG TGGATTCTCA GAACTTAAGT
    TCAAGAAAGA GCCCAGTCCC AGCTCAGACA GCTATACCTG CACCAAAGAT GTGGAAGAAA
    CCAAAAGACC GGACCCGAGC CACTGAAGAG TCGTTAGAGG CAGAATCAGA GCCTAAAGCT
    GAAGAGGAGC TTTCAGGTGA CAAAATACTT GAATCTGAAC AGGATAAAAT GAGCCAAGGG
    TTTCATCCTG AAAGAGACCC CTCTGACCTA AAAAACGTGA AAACTGTGGA AGAAAATGGA
    GAAGAAGCTG AGCCTGTACG TAATGGTGCT GAGAGTATTT CTGAGGGTGA AGGAATAGAT
    GCTAATTCAG GCTCCACTGA TAGTTCTGGT GAAGGGGTCA CATTCCCATT TAAGTCAGAA
    TCCTGGAAGC CTCCTGATTC GGAAGGCAAG AAGCAGTATG ACAGGGAGTT TTTGCTGGAT
    TTCCAATTCA TGCCAGCGTG TATTCAGAAA CCAGAGGGCC TGCCTCCCAT CAGTGATGTG
    GTTCTTGACA AGATCAATCA ACCAAAATTG CCAATGCGAA CTCTGGATCC TCGGATTTTG
    CCTCGAGGAC CAGACTTTAC TCCAGCCTTT GCAGATTTTG GAAGGCAAGC CCCTGGTGGA
    AGAGGTGTAC CTGTTTGCAA AGTGCAGAGC AGGCATGGAT TGCCAATTCT GGAACAGAGC
    AAAGCCCCAG CTTCCACTCC ACTGGCGATG TCGCACCCAC CCCTGAAGAG CCTGCCTCTA
    GGGCTGTTGA ATGTTGGAGC ACGGAGATCT CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG
    ATCATCACGG TCTCCGTGAA AGAAGACGTG CACCTGAAGA AGGCAGAGAA CGCCTGGAAG
    CCCAGCCAGA GGCGGGATAG CCCAGCCGAG GACCCCGAGA ACATCAAGAC CCAGGAACTT
    TTTAGAAAAG TTCGAAGTAT CTTAAATAAA TTGACACCAC AGATGTTCAA CCAACTGATG
    AAGCAAGTGT CAGGACTTAC TGTTGACACA GAGGAGCGGC TGAAAGGAGT CATTGACCTG
    GTCTTTGAGA AGGCTATTGA CGAACCCAGT TTCTCTGTGG CTTACGCAAA CATGTGTCGA
    TGTCTAGTAA CGCTGAAAGT ACCCATGGCA GACAAGCCTG GTAACACAGT GAACTTCCGG
    AAGCTACTAC TGAACCGTTG CCAGAAGGAA TTTGAAAAAG ATAAAGCCGA TGATGATGTC
    TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT GCCAGTGCTC CAGAGGAGAG GACAAGGCTT
    CATGATGAAC TGGAAGAAGC TAAAGATAAA GCCCGGCGGA GATCCATTGG CAACATCAAG
    TTCATTGGAG AACTCTTTAA ACTCAAAATG CTGACTGAAG CCATCATGCA TGACTGTGTG
    GTGAAGCTGC TGAAGAACCA CGATGAAGAA TCCCTCGAGT GTCTGTGTCG CCTGCTCACC
    ACAATTGGCA AAGACCTGGA CTTTGAGAAA GCAAAGCCAC GTATGGACCA GTATTTTAAT
    CAGATGGAGA AAATTGTAAA AGAAAGAAAA ACCTCATCTA GGATTCGATT CATGCTTCAG
    GACGTAATAG ATCTAAGGCT GTGTAATTGG GTATCACGAC GAGCAGATCA GGGGCCTAAA
    ACTATTGAAC AGATTCACAA AGAAGCTAAA ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC
    CAACAACTCA TGACCAAAGA GAAGAGAAGA CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG
    TGGAACACTG TTCAAGGGGC CAAGAATAGT CGGGTATTGG ACCCCTCAAA ATTTCTGAAA
    ATCACTAAGC CCACAATTGA TGAGAAAATT CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC
    TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG GCGAGCGAGG CCGATGCCTT AAGATCAAGT
    GCTTCCAGTT TAAACAGATT CTCTGCTTTG CAACCTCCAG CACCTTCAGG GTCTGCGTCA
    TCCACACCTC TAGAGTTTGA TTCCCGGCGG ACCTTAACTA GTCGAGGAAG CACGGGCAGG
    GAGAAGAATG ACAAGCCCCT CCAGCCTGCA GCAGCTCGGC CGAACACTTT CATGAGGGGT
    GGCAGCAGTA AAGACCTGTT AGACAATCAG TCTCAGGAGG AGCAGCGGAG GGAGATGCTG
    GAGACCGTGA AGCAGCTCAC AGGAGGCATG GACGTGGAGA GGAGCAGCGC TGAGGCGGAG
    CGAAGCAGGA CCAGGGAGCC AGTGACAAAA CCGGAAATTC CAGTAACATC AGCCTCTGAC
    AAGCCCGCGT TGTCAGAGGA AGAGATGGAG AGGAAGTCCA AATCTATCAT TGATGAGTTT
    CTACACATTA ATGATTTTAA GGAAGCCATG CAGTGTGTGG AAGAGCTGAA CGCCCAGGGC
    CTGCAACATG TGTTCGTGAG GACGGGAGTG GAGTCCACCC TGGAAAGGAG CCAGATCACC
    CGGGATCACA TGGGCCAGTT ACTGTATCAG CTGGTGCAGT CAGAAAAGCT CAGCAAACAG
    GACTTTTTCA GAGGTTTTTC AGAAACATTG GAATTGGCAG ATGACATGGC CATTGATATT
    CCCCATATTT GGTTGTACCT TGCTGAACTG GTGACTCCCA TGTTAAAAGA AGGCGGAATC
    TCCATGAGAC AACTCATCAC AGAATTTTGC AAACCTTTAC TTCCTGTTGG AAGAGCTGGA
    ATCTTGATTT CTGAAATATT GCACCTTCTA TGCAAACAAA TGAGCCATAA GAAAGTGGGA
    GCCCTATGGA GGGAGGCTGA CCTCAGCTGG AAGGACTTTT TACCAGAAGG GGAAGATGTA
    CATAATTTTC TCTTGGAGCA GAAGTTGGAC TTTTTAGAGT CTGACAGTTC CTGTTCCTCC
    GAAGCACTTT TGAAGAAAGA ACTCTCTGCC GAAGAGCTGT ATAAGCGACT CGAGAAACTC
    ATTATTGAGG ACAAGGCAAA TGATGAACAG ATCTTTGACT GGGTAGAGGC TAATCTAGAT
    GAAAGCCAGA TGAGTTCACC TACATTCCTT AGAGCTTTAA TGACAGCCGT CTGTAAAGCA
    GCTATTATAG CCGACTGTTC GACCTTCCGA GTGGACACTG CTGTTATCAA GCAGAGAGTG
    CCGATCTTAC TCAAGTACCT AGACTCAGAT ACAGAGAAGG AACTGCAAGC ACTTTATGCA
    CTACAAGCAT CAATAGTAAA ACTTGATCAA CCTGCCAATT TGCTACGGAT GTTTTTTGAT
    TGCCTGTATG ACGAGGAGGT GATCTCTGAG GATGCCTTCT ACAAGTGGGA GAGCAGCAAG
    GACCCGGCAG AGCAGAATGG GAAGGGTGTG GCCCTGAAGT CTGTCACGGC GTTCTTCACA
    TGGCTGCGGG AAGCAGAAGA GGAGTCTGAG GATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88091
    Clone ID Related Accession (Same CDS sequence) XM_015460872.1 , XM_015462474.1 , XM_015460872.2
    Accession Version XM_015460872.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316358.1
    CDS162..5147
    Translation

    Target ORF information:

    RefSeq Version XM_015460872.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88091
    Clone ID Related Accession (Same CDS sequence) XM_015460872.1 , XM_015462474.1 , XM_015460872.2
    Accession Version XM_015462474.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317960.1
    CDS162..5147
    Translation

    Target ORF information:

    RefSeq Version XM_015462474.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462474.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88091
    Clone ID Related Accession (Same CDS sequence) XM_015460872.1 , XM_015462474.1 , XM_015460872.2
    Accession Version XM_015460872.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460872.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316358.1
    CDS196..5181
    Translation

    Target ORF information:

    RefSeq Version XM_015460872.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460872.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88092
    Clone ID Related Accession (Same CDS sequence) XM_015460874.2 , XM_015460874.1 , XM_015462475.1
    Accession Version XM_015460874.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460874.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGCACCTT CAGGGTCTGC GTCATCCACA CCTCTAGAGT TTGATTCCCG GCGGACCTTA
    ACTAGTCGAG GAAGCACGGG CAGGGAGAAG AATGACAAGC CCCTCCAGCC TGCAGCAGCT
    CGGCCGAACA CTTTCATGAG GGGTGGCAGC AGTAAAGACC TGTTAGACAA TCAGTCTCAG
    GAGGAGCAGC GGAGGGAGAT GCTGGAGACC GTGAAGCAGC TCACAGGAGG CATGGACGTG
    GAGAGGAGCA GCGCTGAGGC GGAGCGAAGC AGGACCAGGG AGCCAGTGAC AAAACCGGAA
    ATTCCAGTAA CATCAGCCTC TGACAAGCCC GCGTTGTCAG AGGAAGAGAT GGAGAGGAAG
    TCCAAATCTA TCATTGATGA GTTTCTACAC ATTAATGATT TTAAGGAAGC CATGCAGTGT
    GTGGAAGAGC TGAACGCCCA GGGCCTGCAA CATGTGTTCG TGAGGACGGG AGTGGAGTCC
    ACCCTGGAAA GGAGCCAGAT CACCCGGGAT CACATGGGCC AGTTACTGTA TCAGCTGGTG
    CAGTCAGAAA AGCTCAGCAA ACAGGACTTT TTCAGAGGTT TTTCAGAAAC ATTGGAATTG
    GCAGATGACA TGGCCATTGA TATTCCCCAT ATTTGGTTGT ACCTTGCTGA ACTGGTGACT
    CCCATGTTAA AAGAAGGCGG AATCTCCATG AGACAACTCA TCACAGAATT TTGCAAACCT
    TTACTTCCTG TTGGAAGAGC TGGAATCTTG ATTTCTGAAA TATTGCACCT TCTATGCAAA
    CAAATGAGCC ATAAGAAAGT GGGAGCCCTA TGGAGGGAGG CTGACCTCAG CTGGAAGGAC
    TTTTTACCAG AAGGGGAAGA TGTACATAAT TTTCTCTTGG AGCAGAAGTT GGACTTTTTA
    GAGTCTGACA GTTCCTGTTC CTCCGAAGCA CTTTTGAAGA AAGAACTCTC TGCCGAAGAG
    CTGTATAAGC GACTCGAGAA ACTCATTATT GAGGACAAGG CAAATGATGA ACAGATCTTT
    GACTGGGTAG AGGCTAATCT AGATGAAAGC CAGATGAGTT CACCTACATT CCTTAGAGCT
    TTAATGACAG CCGTCTGTAA AGCAGCTATT ATAGCCGACT GTTCGACCTT CCGAGTGGAC
    ACTGCTGTTA TCAAGCAGAG AGTGCCGATC TTACTCAAGT ACCTAGACTC AGATACAGAG
    AAGGAACTGC AAGCACTTTA TGCACTACAA GCATCAATAG TAAAACTTGA TCAACCTGCC
    AATTTGCTAC GGATGTTTTT TGATTGCCTG TATGACGAGG AGGTGATCTC TGAGGATGCC
    TTCTACAAGT GGGAGAGCAG CAAGGACCCG GCAGAGCAGA ATGGGAAGGG TGTGGCCCTG
    AAGTCTGTCA CGGCGTTCTT CACATGGCTG CGGGAAGCAG AAGAGGAGTC TGAGGATAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316360.1
    CDS196..5178
    Translation

    Target ORF information:

    RefSeq Version XM_015460874.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460874.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGCACCTT CAGGGTCTGC GTCATCCACA CCTCTAGAGT TTGATTCCCG GCGGACCTTA
    ACTAGTCGAG GAAGCACGGG CAGGGAGAAG AATGACAAGC CCCTCCAGCC TGCAGCAGCT
    CGGCCGAACA CTTTCATGAG GGGTGGCAGC AGTAAAGACC TGTTAGACAA TCAGTCTCAG
    GAGGAGCAGC GGAGGGAGAT GCTGGAGACC GTGAAGCAGC TCACAGGAGG CATGGACGTG
    GAGAGGAGCA GCGCTGAGGC GGAGCGAAGC AGGACCAGGG AGCCAGTGAC AAAACCGGAA
    ATTCCAGTAA CATCAGCCTC TGACAAGCCC GCGTTGTCAG AGGAAGAGAT GGAGAGGAAG
    TCCAAATCTA TCATTGATGA GTTTCTACAC ATTAATGATT TTAAGGAAGC CATGCAGTGT
    GTGGAAGAGC TGAACGCCCA GGGCCTGCAA CATGTGTTCG TGAGGACGGG AGTGGAGTCC
    ACCCTGGAAA GGAGCCAGAT CACCCGGGAT CACATGGGCC AGTTACTGTA TCAGCTGGTG
    CAGTCAGAAA AGCTCAGCAA ACAGGACTTT TTCAGAGGTT TTTCAGAAAC ATTGGAATTG
    GCAGATGACA TGGCCATTGA TATTCCCCAT ATTTGGTTGT ACCTTGCTGA ACTGGTGACT
    CCCATGTTAA AAGAAGGCGG AATCTCCATG AGACAACTCA TCACAGAATT TTGCAAACCT
    TTACTTCCTG TTGGAAGAGC TGGAATCTTG ATTTCTGAAA TATTGCACCT TCTATGCAAA
    CAAATGAGCC ATAAGAAAGT GGGAGCCCTA TGGAGGGAGG CTGACCTCAG CTGGAAGGAC
    TTTTTACCAG AAGGGGAAGA TGTACATAAT TTTCTCTTGG AGCAGAAGTT GGACTTTTTA
    GAGTCTGACA GTTCCTGTTC CTCCGAAGCA CTTTTGAAGA AAGAACTCTC TGCCGAAGAG
    CTGTATAAGC GACTCGAGAA ACTCATTATT GAGGACAAGG CAAATGATGA ACAGATCTTT
    GACTGGGTAG AGGCTAATCT AGATGAAAGC CAGATGAGTT CACCTACATT CCTTAGAGCT
    TTAATGACAG CCGTCTGTAA AGCAGCTATT ATAGCCGACT GTTCGACCTT CCGAGTGGAC
    ACTGCTGTTA TCAAGCAGAG AGTGCCGATC TTACTCAAGT ACCTAGACTC AGATACAGAG
    AAGGAACTGC AAGCACTTTA TGCACTACAA GCATCAATAG TAAAACTTGA TCAACCTGCC
    AATTTGCTAC GGATGTTTTT TGATTGCCTG TATGACGAGG AGGTGATCTC TGAGGATGCC
    TTCTACAAGT GGGAGAGCAG CAAGGACCCG GCAGAGCAGA ATGGGAAGGG TGTGGCCCTG
    AAGTCTGTCA CGGCGTTCTT CACATGGCTG CGGGAAGCAG AAGAGGAGTC TGAGGATAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88092
    Clone ID Related Accession (Same CDS sequence) XM_015460874.2 , XM_015460874.1 , XM_015462475.1
    Accession Version XM_015460874.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGCACCTT CAGGGTCTGC GTCATCCACA CCTCTAGAGT TTGATTCCCG GCGGACCTTA
    ACTAGTCGAG GAAGCACGGG CAGGGAGAAG AATGACAAGC CCCTCCAGCC TGCAGCAGCT
    CGGCCGAACA CTTTCATGAG GGGTGGCAGC AGTAAAGACC TGTTAGACAA TCAGTCTCAG
    GAGGAGCAGC GGAGGGAGAT GCTGGAGACC GTGAAGCAGC TCACAGGAGG CATGGACGTG
    GAGAGGAGCA GCGCTGAGGC GGAGCGAAGC AGGACCAGGG AGCCAGTGAC AAAACCGGAA
    ATTCCAGTAA CATCAGCCTC TGACAAGCCC GCGTTGTCAG AGGAAGAGAT GGAGAGGAAG
    TCCAAATCTA TCATTGATGA GTTTCTACAC ATTAATGATT TTAAGGAAGC CATGCAGTGT
    GTGGAAGAGC TGAACGCCCA GGGCCTGCAA CATGTGTTCG TGAGGACGGG AGTGGAGTCC
    ACCCTGGAAA GGAGCCAGAT CACCCGGGAT CACATGGGCC AGTTACTGTA TCAGCTGGTG
    CAGTCAGAAA AGCTCAGCAA ACAGGACTTT TTCAGAGGTT TTTCAGAAAC ATTGGAATTG
    GCAGATGACA TGGCCATTGA TATTCCCCAT ATTTGGTTGT ACCTTGCTGA ACTGGTGACT
    CCCATGTTAA AAGAAGGCGG AATCTCCATG AGACAACTCA TCACAGAATT TTGCAAACCT
    TTACTTCCTG TTGGAAGAGC TGGAATCTTG ATTTCTGAAA TATTGCACCT TCTATGCAAA
    CAAATGAGCC ATAAGAAAGT GGGAGCCCTA TGGAGGGAGG CTGACCTCAG CTGGAAGGAC
    TTTTTACCAG AAGGGGAAGA TGTACATAAT TTTCTCTTGG AGCAGAAGTT GGACTTTTTA
    GAGTCTGACA GTTCCTGTTC CTCCGAAGCA CTTTTGAAGA AAGAACTCTC TGCCGAAGAG
    CTGTATAAGC GACTCGAGAA ACTCATTATT GAGGACAAGG CAAATGATGA ACAGATCTTT
    GACTGGGTAG AGGCTAATCT AGATGAAAGC CAGATGAGTT CACCTACATT CCTTAGAGCT
    TTAATGACAG CCGTCTGTAA AGCAGCTATT ATAGCCGACT GTTCGACCTT CCGAGTGGAC
    ACTGCTGTTA TCAAGCAGAG AGTGCCGATC TTACTCAAGT ACCTAGACTC AGATACAGAG
    AAGGAACTGC AAGCACTTTA TGCACTACAA GCATCAATAG TAAAACTTGA TCAACCTGCC
    AATTTGCTAC GGATGTTTTT TGATTGCCTG TATGACGAGG AGGTGATCTC TGAGGATGCC
    TTCTACAAGT GGGAGAGCAG CAAGGACCCG GCAGAGCAGA ATGGGAAGGG TGTGGCCCTG
    AAGTCTGTCA CGGCGTTCTT CACATGGCTG CGGGAAGCAG AAGAGGAGTC TGAGGATAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316360.1
    CDS162..5144
    Translation

    Target ORF information:

    RefSeq Version XM_015460874.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460874.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGCACCTT CAGGGTCTGC GTCATCCACA CCTCTAGAGT TTGATTCCCG GCGGACCTTA
    ACTAGTCGAG GAAGCACGGG CAGGGAGAAG AATGACAAGC CCCTCCAGCC TGCAGCAGCT
    CGGCCGAACA CTTTCATGAG GGGTGGCAGC AGTAAAGACC TGTTAGACAA TCAGTCTCAG
    GAGGAGCAGC GGAGGGAGAT GCTGGAGACC GTGAAGCAGC TCACAGGAGG CATGGACGTG
    GAGAGGAGCA GCGCTGAGGC GGAGCGAAGC AGGACCAGGG AGCCAGTGAC AAAACCGGAA
    ATTCCAGTAA CATCAGCCTC TGACAAGCCC GCGTTGTCAG AGGAAGAGAT GGAGAGGAAG
    TCCAAATCTA TCATTGATGA GTTTCTACAC ATTAATGATT TTAAGGAAGC CATGCAGTGT
    GTGGAAGAGC TGAACGCCCA GGGCCTGCAA CATGTGTTCG TGAGGACGGG AGTGGAGTCC
    ACCCTGGAAA GGAGCCAGAT CACCCGGGAT CACATGGGCC AGTTACTGTA TCAGCTGGTG
    CAGTCAGAAA AGCTCAGCAA ACAGGACTTT TTCAGAGGTT TTTCAGAAAC ATTGGAATTG
    GCAGATGACA TGGCCATTGA TATTCCCCAT ATTTGGTTGT ACCTTGCTGA ACTGGTGACT
    CCCATGTTAA AAGAAGGCGG AATCTCCATG AGACAACTCA TCACAGAATT TTGCAAACCT
    TTACTTCCTG TTGGAAGAGC TGGAATCTTG ATTTCTGAAA TATTGCACCT TCTATGCAAA
    CAAATGAGCC ATAAGAAAGT GGGAGCCCTA TGGAGGGAGG CTGACCTCAG CTGGAAGGAC
    TTTTTACCAG AAGGGGAAGA TGTACATAAT TTTCTCTTGG AGCAGAAGTT GGACTTTTTA
    GAGTCTGACA GTTCCTGTTC CTCCGAAGCA CTTTTGAAGA AAGAACTCTC TGCCGAAGAG
    CTGTATAAGC GACTCGAGAA ACTCATTATT GAGGACAAGG CAAATGATGA ACAGATCTTT
    GACTGGGTAG AGGCTAATCT AGATGAAAGC CAGATGAGTT CACCTACATT CCTTAGAGCT
    TTAATGACAG CCGTCTGTAA AGCAGCTATT ATAGCCGACT GTTCGACCTT CCGAGTGGAC
    ACTGCTGTTA TCAAGCAGAG AGTGCCGATC TTACTCAAGT ACCTAGACTC AGATACAGAG
    AAGGAACTGC AAGCACTTTA TGCACTACAA GCATCAATAG TAAAACTTGA TCAACCTGCC
    AATTTGCTAC GGATGTTTTT TGATTGCCTG TATGACGAGG AGGTGATCTC TGAGGATGCC
    TTCTACAAGT GGGAGAGCAG CAAGGACCCG GCAGAGCAGA ATGGGAAGGG TGTGGCCCTG
    AAGTCTGTCA CGGCGTTCTT CACATGGCTG CGGGAAGCAG AAGAGGAGTC TGAGGATAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88092
    Clone ID Related Accession (Same CDS sequence) XM_015460874.2 , XM_015460874.1 , XM_015462475.1
    Accession Version XM_015462475.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGCACCTT CAGGGTCTGC GTCATCCACA CCTCTAGAGT TTGATTCCCG GCGGACCTTA
    ACTAGTCGAG GAAGCACGGG CAGGGAGAAG AATGACAAGC CCCTCCAGCC TGCAGCAGCT
    CGGCCGAACA CTTTCATGAG GGGTGGCAGC AGTAAAGACC TGTTAGACAA TCAGTCTCAG
    GAGGAGCAGC GGAGGGAGAT GCTGGAGACC GTGAAGCAGC TCACAGGAGG CATGGACGTG
    GAGAGGAGCA GCGCTGAGGC GGAGCGAAGC AGGACCAGGG AGCCAGTGAC AAAACCGGAA
    ATTCCAGTAA CATCAGCCTC TGACAAGCCC GCGTTGTCAG AGGAAGAGAT GGAGAGGAAG
    TCCAAATCTA TCATTGATGA GTTTCTACAC ATTAATGATT TTAAGGAAGC CATGCAGTGT
    GTGGAAGAGC TGAACGCCCA GGGCCTGCAA CATGTGTTCG TGAGGACGGG AGTGGAGTCC
    ACCCTGGAAA GGAGCCAGAT CACCCGGGAT CACATGGGCC AGTTACTGTA TCAGCTGGTG
    CAGTCAGAAA AGCTCAGCAA ACAGGACTTT TTCAGAGGTT TTTCAGAAAC ATTGGAATTG
    GCAGATGACA TGGCCATTGA TATTCCCCAT ATTTGGTTGT ACCTTGCTGA ACTGGTGACT
    CCCATGTTAA AAGAAGGCGG AATCTCCATG AGACAACTCA TCACAGAATT TTGCAAACCT
    TTACTTCCTG TTGGAAGAGC TGGAATCTTG ATTTCTGAAA TATTGCACCT TCTATGCAAA
    CAAATGAGCC ATAAGAAAGT GGGAGCCCTA TGGAGGGAGG CTGACCTCAG CTGGAAGGAC
    TTTTTACCAG AAGGGGAAGA TGTACATAAT TTTCTCTTGG AGCAGAAGTT GGACTTTTTA
    GAGTCTGACA GTTCCTGTTC CTCCGAAGCA CTTTTGAAGA AAGAACTCTC TGCCGAAGAG
    CTGTATAAGC GACTCGAGAA ACTCATTATT GAGGACAAGG CAAATGATGA ACAGATCTTT
    GACTGGGTAG AGGCTAATCT AGATGAAAGC CAGATGAGTT CACCTACATT CCTTAGAGCT
    TTAATGACAG CCGTCTGTAA AGCAGCTATT ATAGCCGACT GTTCGACCTT CCGAGTGGAC
    ACTGCTGTTA TCAAGCAGAG AGTGCCGATC TTACTCAAGT ACCTAGACTC AGATACAGAG
    AAGGAACTGC AAGCACTTTA TGCACTACAA GCATCAATAG TAAAACTTGA TCAACCTGCC
    AATTTGCTAC GGATGTTTTT TGATTGCCTG TATGACGAGG AGGTGATCTC TGAGGATGCC
    TTCTACAAGT GGGAGAGCAG CAAGGACCCG GCAGAGCAGA ATGGGAAGGG TGTGGCCCTG
    AAGTCTGTCA CGGCGTTCTT CACATGGCTG CGGGAAGCAG AAGAGGAGTC TGAGGATAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317961.1
    CDS162..5144
    Translation

    Target ORF information:

    RefSeq Version XM_015462475.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462475.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGCACCTT CAGGGTCTGC GTCATCCACA CCTCTAGAGT TTGATTCCCG GCGGACCTTA
    ACTAGTCGAG GAAGCACGGG CAGGGAGAAG AATGACAAGC CCCTCCAGCC TGCAGCAGCT
    CGGCCGAACA CTTTCATGAG GGGTGGCAGC AGTAAAGACC TGTTAGACAA TCAGTCTCAG
    GAGGAGCAGC GGAGGGAGAT GCTGGAGACC GTGAAGCAGC TCACAGGAGG CATGGACGTG
    GAGAGGAGCA GCGCTGAGGC GGAGCGAAGC AGGACCAGGG AGCCAGTGAC AAAACCGGAA
    ATTCCAGTAA CATCAGCCTC TGACAAGCCC GCGTTGTCAG AGGAAGAGAT GGAGAGGAAG
    TCCAAATCTA TCATTGATGA GTTTCTACAC ATTAATGATT TTAAGGAAGC CATGCAGTGT
    GTGGAAGAGC TGAACGCCCA GGGCCTGCAA CATGTGTTCG TGAGGACGGG AGTGGAGTCC
    ACCCTGGAAA GGAGCCAGAT CACCCGGGAT CACATGGGCC AGTTACTGTA TCAGCTGGTG
    CAGTCAGAAA AGCTCAGCAA ACAGGACTTT TTCAGAGGTT TTTCAGAAAC ATTGGAATTG
    GCAGATGACA TGGCCATTGA TATTCCCCAT ATTTGGTTGT ACCTTGCTGA ACTGGTGACT
    CCCATGTTAA AAGAAGGCGG AATCTCCATG AGACAACTCA TCACAGAATT TTGCAAACCT
    TTACTTCCTG TTGGAAGAGC TGGAATCTTG ATTTCTGAAA TATTGCACCT TCTATGCAAA
    CAAATGAGCC ATAAGAAAGT GGGAGCCCTA TGGAGGGAGG CTGACCTCAG CTGGAAGGAC
    TTTTTACCAG AAGGGGAAGA TGTACATAAT TTTCTCTTGG AGCAGAAGTT GGACTTTTTA
    GAGTCTGACA GTTCCTGTTC CTCCGAAGCA CTTTTGAAGA AAGAACTCTC TGCCGAAGAG
    CTGTATAAGC GACTCGAGAA ACTCATTATT GAGGACAAGG CAAATGATGA ACAGATCTTT
    GACTGGGTAG AGGCTAATCT AGATGAAAGC CAGATGAGTT CACCTACATT CCTTAGAGCT
    TTAATGACAG CCGTCTGTAA AGCAGCTATT ATAGCCGACT GTTCGACCTT CCGAGTGGAC
    ACTGCTGTTA TCAAGCAGAG AGTGCCGATC TTACTCAAGT ACCTAGACTC AGATACAGAG
    AAGGAACTGC AAGCACTTTA TGCACTACAA GCATCAATAG TAAAACTTGA TCAACCTGCC
    AATTTGCTAC GGATGTTTTT TGATTGCCTG TATGACGAGG AGGTGATCTC TGAGGATGCC
    TTCTACAAGT GGGAGAGCAG CAAGGACCCG GCAGAGCAGA ATGGGAAGGG TGTGGCCCTG
    AAGTCTGTCA CGGCGTTCTT CACATGGCTG CGGGAAGCAG AAGAGGAGTC TGAGGATAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88093
    Clone ID Related Accession (Same CDS sequence) XM_015462476.1 , XM_015460876.1 , XM_015460876.2
    Accession Version XM_015462476.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTCTGTTGA ATGTTGGAGC ACGGAGATCT
    CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG ATCATCACGG TCTCCGTGAA AGAAGACGTG
    CACCTGAAGA AGGCAGAGAA CGCCTGGAAG CCCAGCCAGA GGCGGGATAG CCCAGCCGAG
    GACCCCGAGA ACATCAAGAC CCAGGAACTT TTTAGAAAAG TTCGAAGTAT CTTAAATAAA
    TTGACACCAC AGATGTTCAA CCAACTGATG AAGCAAGTGT CAGGACTTAC TGTTGACACA
    GAGGAGCGGC TGAAAGGAGT CATTGACCTG GTCTTTGAGA AGGCTATTGA CGAACCCAGT
    TTCTCTGTGG CTTACGCAAA CATGTGTCGA TGTCTAGTAA CGCTGAAAGT ACCCATGGCA
    GACAAGCCTG GTAACACAGT GAACTTCCGG AAGCTACTAC TGAACCGTTG CCAGAAGGAA
    TTTGAAAAAG ATAAAGCCGA TGATGATGTC TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT
    GCCAGTGCTC CAGAGGAGAG GACAAGGCTT CATGATGAAC TGGAAGAAGC TAAAGATAAA
    GCCCGGCGGA GATCCATTGG CAACATCAAG TTCATTGGAG AACTCTTTAA ACTCAAAATG
    CTGACTGAAG CCATCATGCA TGACTGTGTG GTGAAGCTGC TGAAGAACCA CGATGAAGAA
    TCCCTCGAGT GTCTGTGTCG CCTGCTCACC ACAATTGGCA AAGACCTGGA CTTTGAGAAA
    GCAAAGCCAC GTATGGACCA GTATTTTAAT CAGATGGAGA AAATTGTAAA AGAAAGAAAA
    ACCTCATCTA GGATTCGATT CATGCTTCAG GACGTAATAG ATCTAAGGCT GTGTAATTGG
    GTATCACGAC GAGCAGATCA GGGGCCTAAA ACTATTGAAC AGATTCACAA AGAAGCTAAA
    ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC CAACAACTCA TGACCAAAGA GAAGAGAAGA
    CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG TGGAACACTG TTCAAGGGGC CAAGAATAGT
    CGGGTATTGG ACCCCTCAAA ATTTCTGAAA ATCACTAAGC CCACAATTGA TGAGAAAATT
    CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG
    GCGAGCGAGG CCGATGCCTT AAGATCAAGT GCTTCCAGTT TAAACAGATT CTCTGCTTTG
    CAACCTCCAG CACCTTCAGG GTCTGCGTCA TCCACACCTC TAGAGTTTGA TTCCCGGCGG
    ACCTTAACTA GTCGAGGAAG CACGGGCAGG GAGAAGAATG ACAAGCCCCT CCAGCCTGCA
    GCAGCTCGGC CGAACACTTT CATGAGGGGT GGCAGCAGTA AAGACCTGTT AGACAATCAG
    TCTCAGGAGG AGCAGCGGAG GGAGATGCTG GAGACCGTGA AGCAGCTCAC AGGAGGCATG
    GACGTGGAGA GGAGCAGCGC TGAGGCGGAG CGAAGCAGGA CCAGGGAGCC AGTGACAAAA
    CCGGAAATTC CAGTAACATC AGCCTCTGAC AAGCCCGCGT TGTCAGAGGA AGAGATGGAG
    AGGAAGTCCA AATCTATCAT TGATGAGTTT CTACACATTA ATGATTTTAA GGAAGCCATG
    CAGTGTGTGG AAGAGCTGAA CGCCCAGGGC CTGCAACATG TGTTCGTGAG GACGGGAGTG
    GAGTCCACCC TGGAAAGGAG CCAGATCACC CGGGATCACA TGGGCCAGTT ACTGTATCAG
    CTGGTGCAGT CAGAAAAGCT CAGCAAACAG GACTTTTTCA GAGGTTTTTC AGAAACATTG
    GAATTGGCAG ATGACATGGC CATTGATATT CCCCATATTT GGTTGTACCT TGCTGAACTG
    GTGACTCCCA TGTTAAAAGA AGGCGGAATC TCCATGAGAC AACTCATCAC AGAATTTTGC
    AAACCTTTAC TTCCTGTTGG AAGAGCTGGA ATCTTGATTT CTGAAATATT GCACCTTCTA
    TGCAAACAAA TGAGCCATAA GAAAGTGGGA GCCCTATGGA GGGAGGCTGA CCTCAGCTGG
    AAGGACTTTT TACCAGAAGG GGAAGATGTA CATAATTTTC TCTTGGAGCA GAAGTTGGAC
    TTTTTAGAGT CTGACAGTTC CTGTTCCTCC GAAGCACTTT TGAAGAAAGA ACTCTCTGCC
    GAAGAGCTGT ATAAGCGACT CGAGAAACTC ATTATTGAGG ACAAGGCAAA TGATGAACAG
    ATCTTTGACT GGGTAGAGGC TAATCTAGAT GAAAGCCAGA TGAGTTCACC TACATTCCTT
    AGAGCTTTAA TGACAGCCGT CTGTAAAGCA GCTATTATAG CCGACTGTTC GACCTTCCGA
    GTGGACACTG CTGTTATCAA GCAGAGAGTG CCGATCTTAC TCAAGTACCT AGACTCAGAT
    ACAGAGAAGG AACTGCAAGC ACTTTATGCA CTACAAGCAT CAATAGTAAA ACTTGATCAA
    CCTGCCAATT TGCTACGGAT GTTTTTTGAT TGCCTGTATG ACGAGGAGGT GATCTCTGAG
    GATGCCTTCT ACAAGTGGGA GAGCAGCAAG GACCCGGCAG AGCAGAATGG GAAGGGTGTG
    GCCCTGAAGT CTGTCACGGC GTTCTTCACA TGGCTGCGGG AAGCAGAAGA GGAGTCTGAG
    GATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317962.1
    CDS162..5090
    Translation

    Target ORF information:

    RefSeq Version XM_015462476.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462476.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTCTGTTGA ATGTTGGAGC ACGGAGATCT
    CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG ATCATCACGG TCTCCGTGAA AGAAGACGTG
    CACCTGAAGA AGGCAGAGAA CGCCTGGAAG CCCAGCCAGA GGCGGGATAG CCCAGCCGAG
    GACCCCGAGA ACATCAAGAC CCAGGAACTT TTTAGAAAAG TTCGAAGTAT CTTAAATAAA
    TTGACACCAC AGATGTTCAA CCAACTGATG AAGCAAGTGT CAGGACTTAC TGTTGACACA
    GAGGAGCGGC TGAAAGGAGT CATTGACCTG GTCTTTGAGA AGGCTATTGA CGAACCCAGT
    TTCTCTGTGG CTTACGCAAA CATGTGTCGA TGTCTAGTAA CGCTGAAAGT ACCCATGGCA
    GACAAGCCTG GTAACACAGT GAACTTCCGG AAGCTACTAC TGAACCGTTG CCAGAAGGAA
    TTTGAAAAAG ATAAAGCCGA TGATGATGTC TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT
    GCCAGTGCTC CAGAGGAGAG GACAAGGCTT CATGATGAAC TGGAAGAAGC TAAAGATAAA
    GCCCGGCGGA GATCCATTGG CAACATCAAG TTCATTGGAG AACTCTTTAA ACTCAAAATG
    CTGACTGAAG CCATCATGCA TGACTGTGTG GTGAAGCTGC TGAAGAACCA CGATGAAGAA
    TCCCTCGAGT GTCTGTGTCG CCTGCTCACC ACAATTGGCA AAGACCTGGA CTTTGAGAAA
    GCAAAGCCAC GTATGGACCA GTATTTTAAT CAGATGGAGA AAATTGTAAA AGAAAGAAAA
    ACCTCATCTA GGATTCGATT CATGCTTCAG GACGTAATAG ATCTAAGGCT GTGTAATTGG
    GTATCACGAC GAGCAGATCA GGGGCCTAAA ACTATTGAAC AGATTCACAA AGAAGCTAAA
    ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC CAACAACTCA TGACCAAAGA GAAGAGAAGA
    CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG TGGAACACTG TTCAAGGGGC CAAGAATAGT
    CGGGTATTGG ACCCCTCAAA ATTTCTGAAA ATCACTAAGC CCACAATTGA TGAGAAAATT
    CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG
    GCGAGCGAGG CCGATGCCTT AAGATCAAGT GCTTCCAGTT TAAACAGATT CTCTGCTTTG
    CAACCTCCAG CACCTTCAGG GTCTGCGTCA TCCACACCTC TAGAGTTTGA TTCCCGGCGG
    ACCTTAACTA GTCGAGGAAG CACGGGCAGG GAGAAGAATG ACAAGCCCCT CCAGCCTGCA
    GCAGCTCGGC CGAACACTTT CATGAGGGGT GGCAGCAGTA AAGACCTGTT AGACAATCAG
    TCTCAGGAGG AGCAGCGGAG GGAGATGCTG GAGACCGTGA AGCAGCTCAC AGGAGGCATG
    GACGTGGAGA GGAGCAGCGC TGAGGCGGAG CGAAGCAGGA CCAGGGAGCC AGTGACAAAA
    CCGGAAATTC CAGTAACATC AGCCTCTGAC AAGCCCGCGT TGTCAGAGGA AGAGATGGAG
    AGGAAGTCCA AATCTATCAT TGATGAGTTT CTACACATTA ATGATTTTAA GGAAGCCATG
    CAGTGTGTGG AAGAGCTGAA CGCCCAGGGC CTGCAACATG TGTTCGTGAG GACGGGAGTG
    GAGTCCACCC TGGAAAGGAG CCAGATCACC CGGGATCACA TGGGCCAGTT ACTGTATCAG
    CTGGTGCAGT CAGAAAAGCT CAGCAAACAG GACTTTTTCA GAGGTTTTTC AGAAACATTG
    GAATTGGCAG ATGACATGGC CATTGATATT CCCCATATTT GGTTGTACCT TGCTGAACTG
    GTGACTCCCA TGTTAAAAGA AGGCGGAATC TCCATGAGAC AACTCATCAC AGAATTTTGC
    AAACCTTTAC TTCCTGTTGG AAGAGCTGGA ATCTTGATTT CTGAAATATT GCACCTTCTA
    TGCAAACAAA TGAGCCATAA GAAAGTGGGA GCCCTATGGA GGGAGGCTGA CCTCAGCTGG
    AAGGACTTTT TACCAGAAGG GGAAGATGTA CATAATTTTC TCTTGGAGCA GAAGTTGGAC
    TTTTTAGAGT CTGACAGTTC CTGTTCCTCC GAAGCACTTT TGAAGAAAGA ACTCTCTGCC
    GAAGAGCTGT ATAAGCGACT CGAGAAACTC ATTATTGAGG ACAAGGCAAA TGATGAACAG
    ATCTTTGACT GGGTAGAGGC TAATCTAGAT GAAAGCCAGA TGAGTTCACC TACATTCCTT
    AGAGCTTTAA TGACAGCCGT CTGTAAAGCA GCTATTATAG CCGACTGTTC GACCTTCCGA
    GTGGACACTG CTGTTATCAA GCAGAGAGTG CCGATCTTAC TCAAGTACCT AGACTCAGAT
    ACAGAGAAGG AACTGCAAGC ACTTTATGCA CTACAAGCAT CAATAGTAAA ACTTGATCAA
    CCTGCCAATT TGCTACGGAT GTTTTTTGAT TGCCTGTATG ACGAGGAGGT GATCTCTGAG
    GATGCCTTCT ACAAGTGGGA GAGCAGCAAG GACCCGGCAG AGCAGAATGG GAAGGGTGTG
    GCCCTGAAGT CTGTCACGGC GTTCTTCACA TGGCTGCGGG AAGCAGAAGA GGAGTCTGAG
    GATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88093
    Clone ID Related Accession (Same CDS sequence) XM_015462476.1 , XM_015460876.1 , XM_015460876.2
    Accession Version XM_015460876.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTCTGTTGA ATGTTGGAGC ACGGAGATCT
    CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG ATCATCACGG TCTCCGTGAA AGAAGACGTG
    CACCTGAAGA AGGCAGAGAA CGCCTGGAAG CCCAGCCAGA GGCGGGATAG CCCAGCCGAG
    GACCCCGAGA ACATCAAGAC CCAGGAACTT TTTAGAAAAG TTCGAAGTAT CTTAAATAAA
    TTGACACCAC AGATGTTCAA CCAACTGATG AAGCAAGTGT CAGGACTTAC TGTTGACACA
    GAGGAGCGGC TGAAAGGAGT CATTGACCTG GTCTTTGAGA AGGCTATTGA CGAACCCAGT
    TTCTCTGTGG CTTACGCAAA CATGTGTCGA TGTCTAGTAA CGCTGAAAGT ACCCATGGCA
    GACAAGCCTG GTAACACAGT GAACTTCCGG AAGCTACTAC TGAACCGTTG CCAGAAGGAA
    TTTGAAAAAG ATAAAGCCGA TGATGATGTC TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT
    GCCAGTGCTC CAGAGGAGAG GACAAGGCTT CATGATGAAC TGGAAGAAGC TAAAGATAAA
    GCCCGGCGGA GATCCATTGG CAACATCAAG TTCATTGGAG AACTCTTTAA ACTCAAAATG
    CTGACTGAAG CCATCATGCA TGACTGTGTG GTGAAGCTGC TGAAGAACCA CGATGAAGAA
    TCCCTCGAGT GTCTGTGTCG CCTGCTCACC ACAATTGGCA AAGACCTGGA CTTTGAGAAA
    GCAAAGCCAC GTATGGACCA GTATTTTAAT CAGATGGAGA AAATTGTAAA AGAAAGAAAA
    ACCTCATCTA GGATTCGATT CATGCTTCAG GACGTAATAG ATCTAAGGCT GTGTAATTGG
    GTATCACGAC GAGCAGATCA GGGGCCTAAA ACTATTGAAC AGATTCACAA AGAAGCTAAA
    ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC CAACAACTCA TGACCAAAGA GAAGAGAAGA
    CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG TGGAACACTG TTCAAGGGGC CAAGAATAGT
    CGGGTATTGG ACCCCTCAAA ATTTCTGAAA ATCACTAAGC CCACAATTGA TGAGAAAATT
    CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG
    GCGAGCGAGG CCGATGCCTT AAGATCAAGT GCTTCCAGTT TAAACAGATT CTCTGCTTTG
    CAACCTCCAG CACCTTCAGG GTCTGCGTCA TCCACACCTC TAGAGTTTGA TTCCCGGCGG
    ACCTTAACTA GTCGAGGAAG CACGGGCAGG GAGAAGAATG ACAAGCCCCT CCAGCCTGCA
    GCAGCTCGGC CGAACACTTT CATGAGGGGT GGCAGCAGTA AAGACCTGTT AGACAATCAG
    TCTCAGGAGG AGCAGCGGAG GGAGATGCTG GAGACCGTGA AGCAGCTCAC AGGAGGCATG
    GACGTGGAGA GGAGCAGCGC TGAGGCGGAG CGAAGCAGGA CCAGGGAGCC AGTGACAAAA
    CCGGAAATTC CAGTAACATC AGCCTCTGAC AAGCCCGCGT TGTCAGAGGA AGAGATGGAG
    AGGAAGTCCA AATCTATCAT TGATGAGTTT CTACACATTA ATGATTTTAA GGAAGCCATG
    CAGTGTGTGG AAGAGCTGAA CGCCCAGGGC CTGCAACATG TGTTCGTGAG GACGGGAGTG
    GAGTCCACCC TGGAAAGGAG CCAGATCACC CGGGATCACA TGGGCCAGTT ACTGTATCAG
    CTGGTGCAGT CAGAAAAGCT CAGCAAACAG GACTTTTTCA GAGGTTTTTC AGAAACATTG
    GAATTGGCAG ATGACATGGC CATTGATATT CCCCATATTT GGTTGTACCT TGCTGAACTG
    GTGACTCCCA TGTTAAAAGA AGGCGGAATC TCCATGAGAC AACTCATCAC AGAATTTTGC
    AAACCTTTAC TTCCTGTTGG AAGAGCTGGA ATCTTGATTT CTGAAATATT GCACCTTCTA
    TGCAAACAAA TGAGCCATAA GAAAGTGGGA GCCCTATGGA GGGAGGCTGA CCTCAGCTGG
    AAGGACTTTT TACCAGAAGG GGAAGATGTA CATAATTTTC TCTTGGAGCA GAAGTTGGAC
    TTTTTAGAGT CTGACAGTTC CTGTTCCTCC GAAGCACTTT TGAAGAAAGA ACTCTCTGCC
    GAAGAGCTGT ATAAGCGACT CGAGAAACTC ATTATTGAGG ACAAGGCAAA TGATGAACAG
    ATCTTTGACT GGGTAGAGGC TAATCTAGAT GAAAGCCAGA TGAGTTCACC TACATTCCTT
    AGAGCTTTAA TGACAGCCGT CTGTAAAGCA GCTATTATAG CCGACTGTTC GACCTTCCGA
    GTGGACACTG CTGTTATCAA GCAGAGAGTG CCGATCTTAC TCAAGTACCT AGACTCAGAT
    ACAGAGAAGG AACTGCAAGC ACTTTATGCA CTACAAGCAT CAATAGTAAA ACTTGATCAA
    CCTGCCAATT TGCTACGGAT GTTTTTTGAT TGCCTGTATG ACGAGGAGGT GATCTCTGAG
    GATGCCTTCT ACAAGTGGGA GAGCAGCAAG GACCCGGCAG AGCAGAATGG GAAGGGTGTG
    GCCCTGAAGT CTGTCACGGC GTTCTTCACA TGGCTGCGGG AAGCAGAAGA GGAGTCTGAG
    GATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316362.1
    CDS162..5090
    Translation

    Target ORF information:

    RefSeq Version XM_015460876.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460876.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTCTGTTGA ATGTTGGAGC ACGGAGATCT
    CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG ATCATCACGG TCTCCGTGAA AGAAGACGTG
    CACCTGAAGA AGGCAGAGAA CGCCTGGAAG CCCAGCCAGA GGCGGGATAG CCCAGCCGAG
    GACCCCGAGA ACATCAAGAC CCAGGAACTT TTTAGAAAAG TTCGAAGTAT CTTAAATAAA
    TTGACACCAC AGATGTTCAA CCAACTGATG AAGCAAGTGT CAGGACTTAC TGTTGACACA
    GAGGAGCGGC TGAAAGGAGT CATTGACCTG GTCTTTGAGA AGGCTATTGA CGAACCCAGT
    TTCTCTGTGG CTTACGCAAA CATGTGTCGA TGTCTAGTAA CGCTGAAAGT ACCCATGGCA
    GACAAGCCTG GTAACACAGT GAACTTCCGG AAGCTACTAC TGAACCGTTG CCAGAAGGAA
    TTTGAAAAAG ATAAAGCCGA TGATGATGTC TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT
    GCCAGTGCTC CAGAGGAGAG GACAAGGCTT CATGATGAAC TGGAAGAAGC TAAAGATAAA
    GCCCGGCGGA GATCCATTGG CAACATCAAG TTCATTGGAG AACTCTTTAA ACTCAAAATG
    CTGACTGAAG CCATCATGCA TGACTGTGTG GTGAAGCTGC TGAAGAACCA CGATGAAGAA
    TCCCTCGAGT GTCTGTGTCG CCTGCTCACC ACAATTGGCA AAGACCTGGA CTTTGAGAAA
    GCAAAGCCAC GTATGGACCA GTATTTTAAT CAGATGGAGA AAATTGTAAA AGAAAGAAAA
    ACCTCATCTA GGATTCGATT CATGCTTCAG GACGTAATAG ATCTAAGGCT GTGTAATTGG
    GTATCACGAC GAGCAGATCA GGGGCCTAAA ACTATTGAAC AGATTCACAA AGAAGCTAAA
    ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC CAACAACTCA TGACCAAAGA GAAGAGAAGA
    CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG TGGAACACTG TTCAAGGGGC CAAGAATAGT
    CGGGTATTGG ACCCCTCAAA ATTTCTGAAA ATCACTAAGC CCACAATTGA TGAGAAAATT
    CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG
    GCGAGCGAGG CCGATGCCTT AAGATCAAGT GCTTCCAGTT TAAACAGATT CTCTGCTTTG
    CAACCTCCAG CACCTTCAGG GTCTGCGTCA TCCACACCTC TAGAGTTTGA TTCCCGGCGG
    ACCTTAACTA GTCGAGGAAG CACGGGCAGG GAGAAGAATG ACAAGCCCCT CCAGCCTGCA
    GCAGCTCGGC CGAACACTTT CATGAGGGGT GGCAGCAGTA AAGACCTGTT AGACAATCAG
    TCTCAGGAGG AGCAGCGGAG GGAGATGCTG GAGACCGTGA AGCAGCTCAC AGGAGGCATG
    GACGTGGAGA GGAGCAGCGC TGAGGCGGAG CGAAGCAGGA CCAGGGAGCC AGTGACAAAA
    CCGGAAATTC CAGTAACATC AGCCTCTGAC AAGCCCGCGT TGTCAGAGGA AGAGATGGAG
    AGGAAGTCCA AATCTATCAT TGATGAGTTT CTACACATTA ATGATTTTAA GGAAGCCATG
    CAGTGTGTGG AAGAGCTGAA CGCCCAGGGC CTGCAACATG TGTTCGTGAG GACGGGAGTG
    GAGTCCACCC TGGAAAGGAG CCAGATCACC CGGGATCACA TGGGCCAGTT ACTGTATCAG
    CTGGTGCAGT CAGAAAAGCT CAGCAAACAG GACTTTTTCA GAGGTTTTTC AGAAACATTG
    GAATTGGCAG ATGACATGGC CATTGATATT CCCCATATTT GGTTGTACCT TGCTGAACTG
    GTGACTCCCA TGTTAAAAGA AGGCGGAATC TCCATGAGAC AACTCATCAC AGAATTTTGC
    AAACCTTTAC TTCCTGTTGG AAGAGCTGGA ATCTTGATTT CTGAAATATT GCACCTTCTA
    TGCAAACAAA TGAGCCATAA GAAAGTGGGA GCCCTATGGA GGGAGGCTGA CCTCAGCTGG
    AAGGACTTTT TACCAGAAGG GGAAGATGTA CATAATTTTC TCTTGGAGCA GAAGTTGGAC
    TTTTTAGAGT CTGACAGTTC CTGTTCCTCC GAAGCACTTT TGAAGAAAGA ACTCTCTGCC
    GAAGAGCTGT ATAAGCGACT CGAGAAACTC ATTATTGAGG ACAAGGCAAA TGATGAACAG
    ATCTTTGACT GGGTAGAGGC TAATCTAGAT GAAAGCCAGA TGAGTTCACC TACATTCCTT
    AGAGCTTTAA TGACAGCCGT CTGTAAAGCA GCTATTATAG CCGACTGTTC GACCTTCCGA
    GTGGACACTG CTGTTATCAA GCAGAGAGTG CCGATCTTAC TCAAGTACCT AGACTCAGAT
    ACAGAGAAGG AACTGCAAGC ACTTTATGCA CTACAAGCAT CAATAGTAAA ACTTGATCAA
    CCTGCCAATT TGCTACGGAT GTTTTTTGAT TGCCTGTATG ACGAGGAGGT GATCTCTGAG
    GATGCCTTCT ACAAGTGGGA GAGCAGCAAG GACCCGGCAG AGCAGAATGG GAAGGGTGTG
    GCCCTGAAGT CTGTCACGGC GTTCTTCACA TGGCTGCGGG AAGCAGAAGA GGAGTCTGAG
    GATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88093
    Clone ID Related Accession (Same CDS sequence) XM_015462476.1 , XM_015460876.1 , XM_015460876.2
    Accession Version XM_015460876.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460876.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTCTGTTGA ATGTTGGAGC ACGGAGATCT
    CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG ATCATCACGG TCTCCGTGAA AGAAGACGTG
    CACCTGAAGA AGGCAGAGAA CGCCTGGAAG CCCAGCCAGA GGCGGGATAG CCCAGCCGAG
    GACCCCGAGA ACATCAAGAC CCAGGAACTT TTTAGAAAAG TTCGAAGTAT CTTAAATAAA
    TTGACACCAC AGATGTTCAA CCAACTGATG AAGCAAGTGT CAGGACTTAC TGTTGACACA
    GAGGAGCGGC TGAAAGGAGT CATTGACCTG GTCTTTGAGA AGGCTATTGA CGAACCCAGT
    TTCTCTGTGG CTTACGCAAA CATGTGTCGA TGTCTAGTAA CGCTGAAAGT ACCCATGGCA
    GACAAGCCTG GTAACACAGT GAACTTCCGG AAGCTACTAC TGAACCGTTG CCAGAAGGAA
    TTTGAAAAAG ATAAAGCCGA TGATGATGTC TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT
    GCCAGTGCTC CAGAGGAGAG GACAAGGCTT CATGATGAAC TGGAAGAAGC TAAAGATAAA
    GCCCGGCGGA GATCCATTGG CAACATCAAG TTCATTGGAG AACTCTTTAA ACTCAAAATG
    CTGACTGAAG CCATCATGCA TGACTGTGTG GTGAAGCTGC TGAAGAACCA CGATGAAGAA
    TCCCTCGAGT GTCTGTGTCG CCTGCTCACC ACAATTGGCA AAGACCTGGA CTTTGAGAAA
    GCAAAGCCAC GTATGGACCA GTATTTTAAT CAGATGGAGA AAATTGTAAA AGAAAGAAAA
    ACCTCATCTA GGATTCGATT CATGCTTCAG GACGTAATAG ATCTAAGGCT GTGTAATTGG
    GTATCACGAC GAGCAGATCA GGGGCCTAAA ACTATTGAAC AGATTCACAA AGAAGCTAAA
    ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC CAACAACTCA TGACCAAAGA GAAGAGAAGA
    CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG TGGAACACTG TTCAAGGGGC CAAGAATAGT
    CGGGTATTGG ACCCCTCAAA ATTTCTGAAA ATCACTAAGC CCACAATTGA TGAGAAAATT
    CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG
    GCGAGCGAGG CCGATGCCTT AAGATCAAGT GCTTCCAGTT TAAACAGATT CTCTGCTTTG
    CAACCTCCAG CACCTTCAGG GTCTGCGTCA TCCACACCTC TAGAGTTTGA TTCCCGGCGG
    ACCTTAACTA GTCGAGGAAG CACGGGCAGG GAGAAGAATG ACAAGCCCCT CCAGCCTGCA
    GCAGCTCGGC CGAACACTTT CATGAGGGGT GGCAGCAGTA AAGACCTGTT AGACAATCAG
    TCTCAGGAGG AGCAGCGGAG GGAGATGCTG GAGACCGTGA AGCAGCTCAC AGGAGGCATG
    GACGTGGAGA GGAGCAGCGC TGAGGCGGAG CGAAGCAGGA CCAGGGAGCC AGTGACAAAA
    CCGGAAATTC CAGTAACATC AGCCTCTGAC AAGCCCGCGT TGTCAGAGGA AGAGATGGAG
    AGGAAGTCCA AATCTATCAT TGATGAGTTT CTACACATTA ATGATTTTAA GGAAGCCATG
    CAGTGTGTGG AAGAGCTGAA CGCCCAGGGC CTGCAACATG TGTTCGTGAG GACGGGAGTG
    GAGTCCACCC TGGAAAGGAG CCAGATCACC CGGGATCACA TGGGCCAGTT ACTGTATCAG
    CTGGTGCAGT CAGAAAAGCT CAGCAAACAG GACTTTTTCA GAGGTTTTTC AGAAACATTG
    GAATTGGCAG ATGACATGGC CATTGATATT CCCCATATTT GGTTGTACCT TGCTGAACTG
    GTGACTCCCA TGTTAAAAGA AGGCGGAATC TCCATGAGAC AACTCATCAC AGAATTTTGC
    AAACCTTTAC TTCCTGTTGG AAGAGCTGGA ATCTTGATTT CTGAAATATT GCACCTTCTA
    TGCAAACAAA TGAGCCATAA GAAAGTGGGA GCCCTATGGA GGGAGGCTGA CCTCAGCTGG
    AAGGACTTTT TACCAGAAGG GGAAGATGTA CATAATTTTC TCTTGGAGCA GAAGTTGGAC
    TTTTTAGAGT CTGACAGTTC CTGTTCCTCC GAAGCACTTT TGAAGAAAGA ACTCTCTGCC
    GAAGAGCTGT ATAAGCGACT CGAGAAACTC ATTATTGAGG ACAAGGCAAA TGATGAACAG
    ATCTTTGACT GGGTAGAGGC TAATCTAGAT GAAAGCCAGA TGAGTTCACC TACATTCCTT
    AGAGCTTTAA TGACAGCCGT CTGTAAAGCA GCTATTATAG CCGACTGTTC GACCTTCCGA
    GTGGACACTG CTGTTATCAA GCAGAGAGTG CCGATCTTAC TCAAGTACCT AGACTCAGAT
    ACAGAGAAGG AACTGCAAGC ACTTTATGCA CTACAAGCAT CAATAGTAAA ACTTGATCAA
    CCTGCCAATT TGCTACGGAT GTTTTTTGAT TGCCTGTATG ACGAGGAGGT GATCTCTGAG
    GATGCCTTCT ACAAGTGGGA GAGCAGCAAG GACCCGGCAG AGCAGAATGG GAAGGGTGTG
    GCCCTGAAGT CTGTCACGGC GTTCTTCACA TGGCTGCGGG AAGCAGAAGA GGAGTCTGAG
    GATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316362.1
    CDS196..5124
    Translation

    Target ORF information:

    RefSeq Version XM_015460876.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460876.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTCTGTTGA ATGTTGGAGC ACGGAGATCT
    CAGCCTGGCC AGAGGAGAGA GCCCAGGAAG ATCATCACGG TCTCCGTGAA AGAAGACGTG
    CACCTGAAGA AGGCAGAGAA CGCCTGGAAG CCCAGCCAGA GGCGGGATAG CCCAGCCGAG
    GACCCCGAGA ACATCAAGAC CCAGGAACTT TTTAGAAAAG TTCGAAGTAT CTTAAATAAA
    TTGACACCAC AGATGTTCAA CCAACTGATG AAGCAAGTGT CAGGACTTAC TGTTGACACA
    GAGGAGCGGC TGAAAGGAGT CATTGACCTG GTCTTTGAGA AGGCTATTGA CGAACCCAGT
    TTCTCTGTGG CTTACGCAAA CATGTGTCGA TGTCTAGTAA CGCTGAAAGT ACCCATGGCA
    GACAAGCCTG GTAACACAGT GAACTTCCGG AAGCTACTAC TGAACCGTTG CCAGAAGGAA
    TTTGAAAAAG ATAAAGCCGA TGATGATGTC TTTGAGAAGA AGCAGAAAGA ACTCGAGGCT
    GCCAGTGCTC CAGAGGAGAG GACAAGGCTT CATGATGAAC TGGAAGAAGC TAAAGATAAA
    GCCCGGCGGA GATCCATTGG CAACATCAAG TTCATTGGAG AACTCTTTAA ACTCAAAATG
    CTGACTGAAG CCATCATGCA TGACTGTGTG GTGAAGCTGC TGAAGAACCA CGATGAAGAA
    TCCCTCGAGT GTCTGTGTCG CCTGCTCACC ACAATTGGCA AAGACCTGGA CTTTGAGAAA
    GCAAAGCCAC GTATGGACCA GTATTTTAAT CAGATGGAGA AAATTGTAAA AGAAAGAAAA
    ACCTCATCTA GGATTCGATT CATGCTTCAG GACGTAATAG ATCTAAGGCT GTGTAATTGG
    GTATCACGAC GAGCAGATCA GGGGCCTAAA ACTATTGAAC AGATTCACAA AGAAGCTAAA
    ATAGAAGAAC AGGAAGAGCA GAGGAAGGTC CAACAACTCA TGACCAAAGA GAAGAGAAGA
    CCAGGTGTCC AGAGAGTGGA TGAAGGTGGG TGGAACACTG TTCAAGGGGC CAAGAATAGT
    CGGGTATTGG ACCCCTCAAA ATTTCTGAAA ATCACTAAGC CCACAATTGA TGAGAAAATT
    CAGCTGGTAC CTAAAGCGCA GCTGGGCAGC TGGGGAAAGG GCAGCAGTGG TGGAGCCAAG
    GCGAGCGAGG CCGATGCCTT AAGATCAAGT GCTTCCAGTT TAAACAGATT CTCTGCTTTG
    CAACCTCCAG CACCTTCAGG GTCTGCGTCA TCCACACCTC TAGAGTTTGA TTCCCGGCGG
    ACCTTAACTA GTCGAGGAAG CACGGGCAGG GAGAAGAATG ACAAGCCCCT CCAGCCTGCA
    GCAGCTCGGC CGAACACTTT CATGAGGGGT GGCAGCAGTA AAGACCTGTT AGACAATCAG
    TCTCAGGAGG AGCAGCGGAG GGAGATGCTG GAGACCGTGA AGCAGCTCAC AGGAGGCATG
    GACGTGGAGA GGAGCAGCGC TGAGGCGGAG CGAAGCAGGA CCAGGGAGCC AGTGACAAAA
    CCGGAAATTC CAGTAACATC AGCCTCTGAC AAGCCCGCGT TGTCAGAGGA AGAGATGGAG
    AGGAAGTCCA AATCTATCAT TGATGAGTTT CTACACATTA ATGATTTTAA GGAAGCCATG
    CAGTGTGTGG AAGAGCTGAA CGCCCAGGGC CTGCAACATG TGTTCGTGAG GACGGGAGTG
    GAGTCCACCC TGGAAAGGAG CCAGATCACC CGGGATCACA TGGGCCAGTT ACTGTATCAG
    CTGGTGCAGT CAGAAAAGCT CAGCAAACAG GACTTTTTCA GAGGTTTTTC AGAAACATTG
    GAATTGGCAG ATGACATGGC CATTGATATT CCCCATATTT GGTTGTACCT TGCTGAACTG
    GTGACTCCCA TGTTAAAAGA AGGCGGAATC TCCATGAGAC AACTCATCAC AGAATTTTGC
    AAACCTTTAC TTCCTGTTGG AAGAGCTGGA ATCTTGATTT CTGAAATATT GCACCTTCTA
    TGCAAACAAA TGAGCCATAA GAAAGTGGGA GCCCTATGGA GGGAGGCTGA CCTCAGCTGG
    AAGGACTTTT TACCAGAAGG GGAAGATGTA CATAATTTTC TCTTGGAGCA GAAGTTGGAC
    TTTTTAGAGT CTGACAGTTC CTGTTCCTCC GAAGCACTTT TGAAGAAAGA ACTCTCTGCC
    GAAGAGCTGT ATAAGCGACT CGAGAAACTC ATTATTGAGG ACAAGGCAAA TGATGAACAG
    ATCTTTGACT GGGTAGAGGC TAATCTAGAT GAAAGCCAGA TGAGTTCACC TACATTCCTT
    AGAGCTTTAA TGACAGCCGT CTGTAAAGCA GCTATTATAG CCGACTGTTC GACCTTCCGA
    GTGGACACTG CTGTTATCAA GCAGAGAGTG CCGATCTTAC TCAAGTACCT AGACTCAGAT
    ACAGAGAAGG AACTGCAAGC ACTTTATGCA CTACAAGCAT CAATAGTAAA ACTTGATCAA
    CCTGCCAATT TGCTACGGAT GTTTTTTGAT TGCCTGTATG ACGAGGAGGT GATCTCTGAG
    GATGCCTTCT ACAAGTGGGA GAGCAGCAAG GACCCGGCAG AGCAGAATGG GAAGGGTGTG
    GCCCTGAAGT CTGTCACGGC GTTCTTCACA TGGCTGCGGG AAGCAGAAGA GGAGTCTGAG
    GATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88094
    Clone ID Related Accession (Same CDS sequence) XM_015460878.1 , XM_015462477.1 , XM_015460878.2
    Accession Version XM_015460878.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCTGCCC
    AGCCAGGTCC CCGAGCACAG CCCTGTGGTC TATGGGACTG TGGAGAGCGC TCATCTTGCT
    GCCAGCACCC CTGTCACTGC AGCGAGCGAC CAGAAGCAAG AAGAGAAGCC AAAACCAGAT
    CCAGTGTTAA AGACTCCTTC CCCAGTCCTT AGGCTAGCCC TCAGTGGAGA GAAGAAAGAA
    CAAGCAGGCC CGACGTCTGA GATTACTGCA GTAGAGTCCA TACCAGAGCT TCCTCTGCCT
    CCATCCCCTA CTGCTATTTC TCCACTTGCT CGAAGTACAA TTACTTCTCC CACCTCTGTT
    GCTCTTAGTA GCCAACCAAT ATTCACCACT GCAATTGATG ACAGATGTGA ACTCTCTTCC
    TCAAAAGAAG ATACAATTCC TATTCCCAGC CCCACATCTT GCACAGAAGC ATCAGATCCT
    TTACCAACAG ATGAAATTGA TGATGATATA TGCAAGAAAT CTTGTAGTGT AGCACCAAAT
    GATATTCCAC TGATTTCTAG TACTAACCTA ATTAATGAAA TGAATGGAGT TAATGAAAAA
    TTACCAGCCA CGGAAAGCAT TGTGGAAATA GTAAAACAGG AGGTGTTACC GCTGACTCTT
    GAATTGGAGA TTTTGGAAAA TCCCCCTGAA GAAATCAAAG TGGAGTGTGT CCCAACTCCC
    ATCACCCCTT CGATAGTTCC CTCCTCTTCT CCGTCTCCTC CAGCTCCTCC AGCTTCTCCT
    TCTCCCACAC CAGTTATCGT TCCTGCTGCT GCCACCAGTG TTAATACTGG TCGTGGTTCC
    ACCGGAGTCC AGAGAGCCTT CGAAGAGGGC GAGAACATAA GAACTTGCCT TAGTGAAGAT
    GCAAAAGAGA TTCAGAACAA AGTAGAGGTA GAAGCAGATG GGCAAACAGA AGAGATTGTG
    GATTCTCAGA ACTTAAGTTC AAGAAAGAGC CCAGTCCCAG CTCAGACAGC TATACCTGCA
    CCAAAGATGT GGAAGAAACC AAAAGACCGG ACCCGAGCCA CTGAAGAGTC GTTAGAGGCA
    GAATCAGAGC CTAAAGCTGA AGAGGAGCTT TCAGGTGACA AAATACTTGA ATCTGAACAG
    GATAAAATGA GCCAAGGGTT TCATCCTGAA AGAGACCCCT CTGACCTAAA AAACGTGAAA
    ACTGTGGAAG AAAATGGAGA AGAAGCTGAG CCTGTACGTA ATGGTGCTGA GAGTATTTCT
    GAGGGTGAAG GAATAGATGC TAATTCAGGC TCCACTGATA GTTCTGGTGA AGGGGTCACA
    TTCCCATTTA AGTCAGAATC CTGGAAGCCT CCTGATTCGG AAGGCAAGAA GCAGTATGAC
    AGGGAGTTTT TGCTGGATTT CCAATTCATG CCAGCGTGTA TTCAGAAACC AGAGGGCCTG
    CCTCCCATCA GTGATGTGGT TCTTGACAAG ATCAATCAAC CAAAATTGCC AATGCGAACT
    CTGGATCCTC GGATTTTGCC TCGAGGACCA GACTTTACTC CAGCCTTTGC AGATTTTGGA
    AGGCAAGCCC CTGGTGGAAG AGGTGTACCT CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316364.1
    CDS162..5087
    Translation

    Target ORF information:

    RefSeq Version XM_015460878.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460878.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCTGCCC
    AGCCAGGTCC CCGAGCACAG CCCTGTGGTC TATGGGACTG TGGAGAGCGC TCATCTTGCT
    GCCAGCACCC CTGTCACTGC AGCGAGCGAC CAGAAGCAAG AAGAGAAGCC AAAACCAGAT
    CCAGTGTTAA AGACTCCTTC CCCAGTCCTT AGGCTAGCCC TCAGTGGAGA GAAGAAAGAA
    CAAGCAGGCC CGACGTCTGA GATTACTGCA GTAGAGTCCA TACCAGAGCT TCCTCTGCCT
    CCATCCCCTA CTGCTATTTC TCCACTTGCT CGAAGTACAA TTACTTCTCC CACCTCTGTT
    GCTCTTAGTA GCCAACCAAT ATTCACCACT GCAATTGATG ACAGATGTGA ACTCTCTTCC
    TCAAAAGAAG ATACAATTCC TATTCCCAGC CCCACATCTT GCACAGAAGC ATCAGATCCT
    TTACCAACAG ATGAAATTGA TGATGATATA TGCAAGAAAT CTTGTAGTGT AGCACCAAAT
    GATATTCCAC TGATTTCTAG TACTAACCTA ATTAATGAAA TGAATGGAGT TAATGAAAAA
    TTACCAGCCA CGGAAAGCAT TGTGGAAATA GTAAAACAGG AGGTGTTACC GCTGACTCTT
    GAATTGGAGA TTTTGGAAAA TCCCCCTGAA GAAATCAAAG TGGAGTGTGT CCCAACTCCC
    ATCACCCCTT CGATAGTTCC CTCCTCTTCT CCGTCTCCTC CAGCTCCTCC AGCTTCTCCT
    TCTCCCACAC CAGTTATCGT TCCTGCTGCT GCCACCAGTG TTAATACTGG TCGTGGTTCC
    ACCGGAGTCC AGAGAGCCTT CGAAGAGGGC GAGAACATAA GAACTTGCCT TAGTGAAGAT
    GCAAAAGAGA TTCAGAACAA AGTAGAGGTA GAAGCAGATG GGCAAACAGA AGAGATTGTG
    GATTCTCAGA ACTTAAGTTC AAGAAAGAGC CCAGTCCCAG CTCAGACAGC TATACCTGCA
    CCAAAGATGT GGAAGAAACC AAAAGACCGG ACCCGAGCCA CTGAAGAGTC GTTAGAGGCA
    GAATCAGAGC CTAAAGCTGA AGAGGAGCTT TCAGGTGACA AAATACTTGA ATCTGAACAG
    GATAAAATGA GCCAAGGGTT TCATCCTGAA AGAGACCCCT CTGACCTAAA AAACGTGAAA
    ACTGTGGAAG AAAATGGAGA AGAAGCTGAG CCTGTACGTA ATGGTGCTGA GAGTATTTCT
    GAGGGTGAAG GAATAGATGC TAATTCAGGC TCCACTGATA GTTCTGGTGA AGGGGTCACA
    TTCCCATTTA AGTCAGAATC CTGGAAGCCT CCTGATTCGG AAGGCAAGAA GCAGTATGAC
    AGGGAGTTTT TGCTGGATTT CCAATTCATG CCAGCGTGTA TTCAGAAACC AGAGGGCCTG
    CCTCCCATCA GTGATGTGGT TCTTGACAAG ATCAATCAAC CAAAATTGCC AATGCGAACT
    CTGGATCCTC GGATTTTGCC TCGAGGACCA GACTTTACTC CAGCCTTTGC AGATTTTGGA
    AGGCAAGCCC CTGGTGGAAG AGGTGTACCT CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88094
    Clone ID Related Accession (Same CDS sequence) XM_015460878.1 , XM_015462477.1 , XM_015460878.2
    Accession Version XM_015462477.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCTGCCC
    AGCCAGGTCC CCGAGCACAG CCCTGTGGTC TATGGGACTG TGGAGAGCGC TCATCTTGCT
    GCCAGCACCC CTGTCACTGC AGCGAGCGAC CAGAAGCAAG AAGAGAAGCC AAAACCAGAT
    CCAGTGTTAA AGACTCCTTC CCCAGTCCTT AGGCTAGCCC TCAGTGGAGA GAAGAAAGAA
    CAAGCAGGCC CGACGTCTGA GATTACTGCA GTAGAGTCCA TACCAGAGCT TCCTCTGCCT
    CCATCCCCTA CTGCTATTTC TCCACTTGCT CGAAGTACAA TTACTTCTCC CACCTCTGTT
    GCTCTTAGTA GCCAACCAAT ATTCACCACT GCAATTGATG ACAGATGTGA ACTCTCTTCC
    TCAAAAGAAG ATACAATTCC TATTCCCAGC CCCACATCTT GCACAGAAGC ATCAGATCCT
    TTACCAACAG ATGAAATTGA TGATGATATA TGCAAGAAAT CTTGTAGTGT AGCACCAAAT
    GATATTCCAC TGATTTCTAG TACTAACCTA ATTAATGAAA TGAATGGAGT TAATGAAAAA
    TTACCAGCCA CGGAAAGCAT TGTGGAAATA GTAAAACAGG AGGTGTTACC GCTGACTCTT
    GAATTGGAGA TTTTGGAAAA TCCCCCTGAA GAAATCAAAG TGGAGTGTGT CCCAACTCCC
    ATCACCCCTT CGATAGTTCC CTCCTCTTCT CCGTCTCCTC CAGCTCCTCC AGCTTCTCCT
    TCTCCCACAC CAGTTATCGT TCCTGCTGCT GCCACCAGTG TTAATACTGG TCGTGGTTCC
    ACCGGAGTCC AGAGAGCCTT CGAAGAGGGC GAGAACATAA GAACTTGCCT TAGTGAAGAT
    GCAAAAGAGA TTCAGAACAA AGTAGAGGTA GAAGCAGATG GGCAAACAGA AGAGATTGTG
    GATTCTCAGA ACTTAAGTTC AAGAAAGAGC CCAGTCCCAG CTCAGACAGC TATACCTGCA
    CCAAAGATGT GGAAGAAACC AAAAGACCGG ACCCGAGCCA CTGAAGAGTC GTTAGAGGCA
    GAATCAGAGC CTAAAGCTGA AGAGGAGCTT TCAGGTGACA AAATACTTGA ATCTGAACAG
    GATAAAATGA GCCAAGGGTT TCATCCTGAA AGAGACCCCT CTGACCTAAA AAACGTGAAA
    ACTGTGGAAG AAAATGGAGA AGAAGCTGAG CCTGTACGTA ATGGTGCTGA GAGTATTTCT
    GAGGGTGAAG GAATAGATGC TAATTCAGGC TCCACTGATA GTTCTGGTGA AGGGGTCACA
    TTCCCATTTA AGTCAGAATC CTGGAAGCCT CCTGATTCGG AAGGCAAGAA GCAGTATGAC
    AGGGAGTTTT TGCTGGATTT CCAATTCATG CCAGCGTGTA TTCAGAAACC AGAGGGCCTG
    CCTCCCATCA GTGATGTGGT TCTTGACAAG ATCAATCAAC CAAAATTGCC AATGCGAACT
    CTGGATCCTC GGATTTTGCC TCGAGGACCA GACTTTACTC CAGCCTTTGC AGATTTTGGA
    AGGCAAGCCC CTGGTGGAAG AGGTGTACCT CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317963.1
    CDS162..5087
    Translation

    Target ORF information:

    RefSeq Version XM_015462477.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462477.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCTGCCC
    AGCCAGGTCC CCGAGCACAG CCCTGTGGTC TATGGGACTG TGGAGAGCGC TCATCTTGCT
    GCCAGCACCC CTGTCACTGC AGCGAGCGAC CAGAAGCAAG AAGAGAAGCC AAAACCAGAT
    CCAGTGTTAA AGACTCCTTC CCCAGTCCTT AGGCTAGCCC TCAGTGGAGA GAAGAAAGAA
    CAAGCAGGCC CGACGTCTGA GATTACTGCA GTAGAGTCCA TACCAGAGCT TCCTCTGCCT
    CCATCCCCTA CTGCTATTTC TCCACTTGCT CGAAGTACAA TTACTTCTCC CACCTCTGTT
    GCTCTTAGTA GCCAACCAAT ATTCACCACT GCAATTGATG ACAGATGTGA ACTCTCTTCC
    TCAAAAGAAG ATACAATTCC TATTCCCAGC CCCACATCTT GCACAGAAGC ATCAGATCCT
    TTACCAACAG ATGAAATTGA TGATGATATA TGCAAGAAAT CTTGTAGTGT AGCACCAAAT
    GATATTCCAC TGATTTCTAG TACTAACCTA ATTAATGAAA TGAATGGAGT TAATGAAAAA
    TTACCAGCCA CGGAAAGCAT TGTGGAAATA GTAAAACAGG AGGTGTTACC GCTGACTCTT
    GAATTGGAGA TTTTGGAAAA TCCCCCTGAA GAAATCAAAG TGGAGTGTGT CCCAACTCCC
    ATCACCCCTT CGATAGTTCC CTCCTCTTCT CCGTCTCCTC CAGCTCCTCC AGCTTCTCCT
    TCTCCCACAC CAGTTATCGT TCCTGCTGCT GCCACCAGTG TTAATACTGG TCGTGGTTCC
    ACCGGAGTCC AGAGAGCCTT CGAAGAGGGC GAGAACATAA GAACTTGCCT TAGTGAAGAT
    GCAAAAGAGA TTCAGAACAA AGTAGAGGTA GAAGCAGATG GGCAAACAGA AGAGATTGTG
    GATTCTCAGA ACTTAAGTTC AAGAAAGAGC CCAGTCCCAG CTCAGACAGC TATACCTGCA
    CCAAAGATGT GGAAGAAACC AAAAGACCGG ACCCGAGCCA CTGAAGAGTC GTTAGAGGCA
    GAATCAGAGC CTAAAGCTGA AGAGGAGCTT TCAGGTGACA AAATACTTGA ATCTGAACAG
    GATAAAATGA GCCAAGGGTT TCATCCTGAA AGAGACCCCT CTGACCTAAA AAACGTGAAA
    ACTGTGGAAG AAAATGGAGA AGAAGCTGAG CCTGTACGTA ATGGTGCTGA GAGTATTTCT
    GAGGGTGAAG GAATAGATGC TAATTCAGGC TCCACTGATA GTTCTGGTGA AGGGGTCACA
    TTCCCATTTA AGTCAGAATC CTGGAAGCCT CCTGATTCGG AAGGCAAGAA GCAGTATGAC
    AGGGAGTTTT TGCTGGATTT CCAATTCATG CCAGCGTGTA TTCAGAAACC AGAGGGCCTG
    CCTCCCATCA GTGATGTGGT TCTTGACAAG ATCAATCAAC CAAAATTGCC AATGCGAACT
    CTGGATCCTC GGATTTTGCC TCGAGGACCA GACTTTACTC CAGCCTTTGC AGATTTTGGA
    AGGCAAGCCC CTGGTGGAAG AGGTGTACCT CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88094
    Clone ID Related Accession (Same CDS sequence) XM_015460878.1 , XM_015462477.1 , XM_015460878.2
    Accession Version XM_015460878.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460878.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCTGCCC
    AGCCAGGTCC CCGAGCACAG CCCTGTGGTC TATGGGACTG TGGAGAGCGC TCATCTTGCT
    GCCAGCACCC CTGTCACTGC AGCGAGCGAC CAGAAGCAAG AAGAGAAGCC AAAACCAGAT
    CCAGTGTTAA AGACTCCTTC CCCAGTCCTT AGGCTAGCCC TCAGTGGAGA GAAGAAAGAA
    CAAGCAGGCC CGACGTCTGA GATTACTGCA GTAGAGTCCA TACCAGAGCT TCCTCTGCCT
    CCATCCCCTA CTGCTATTTC TCCACTTGCT CGAAGTACAA TTACTTCTCC CACCTCTGTT
    GCTCTTAGTA GCCAACCAAT ATTCACCACT GCAATTGATG ACAGATGTGA ACTCTCTTCC
    TCAAAAGAAG ATACAATTCC TATTCCCAGC CCCACATCTT GCACAGAAGC ATCAGATCCT
    TTACCAACAG ATGAAATTGA TGATGATATA TGCAAGAAAT CTTGTAGTGT AGCACCAAAT
    GATATTCCAC TGATTTCTAG TACTAACCTA ATTAATGAAA TGAATGGAGT TAATGAAAAA
    TTACCAGCCA CGGAAAGCAT TGTGGAAATA GTAAAACAGG AGGTGTTACC GCTGACTCTT
    GAATTGGAGA TTTTGGAAAA TCCCCCTGAA GAAATCAAAG TGGAGTGTGT CCCAACTCCC
    ATCACCCCTT CGATAGTTCC CTCCTCTTCT CCGTCTCCTC CAGCTCCTCC AGCTTCTCCT
    TCTCCCACAC CAGTTATCGT TCCTGCTGCT GCCACCAGTG TTAATACTGG TCGTGGTTCC
    ACCGGAGTCC AGAGAGCCTT CGAAGAGGGC GAGAACATAA GAACTTGCCT TAGTGAAGAT
    GCAAAAGAGA TTCAGAACAA AGTAGAGGTA GAAGCAGATG GGCAAACAGA AGAGATTGTG
    GATTCTCAGA ACTTAAGTTC AAGAAAGAGC CCAGTCCCAG CTCAGACAGC TATACCTGCA
    CCAAAGATGT GGAAGAAACC AAAAGACCGG ACCCGAGCCA CTGAAGAGTC GTTAGAGGCA
    GAATCAGAGC CTAAAGCTGA AGAGGAGCTT TCAGGTGACA AAATACTTGA ATCTGAACAG
    GATAAAATGA GCCAAGGGTT TCATCCTGAA AGAGACCCCT CTGACCTAAA AAACGTGAAA
    ACTGTGGAAG AAAATGGAGA AGAAGCTGAG CCTGTACGTA ATGGTGCTGA GAGTATTTCT
    GAGGGTGAAG GAATAGATGC TAATTCAGGC TCCACTGATA GTTCTGGTGA AGGGGTCACA
    TTCCCATTTA AGTCAGAATC CTGGAAGCCT CCTGATTCGG AAGGCAAGAA GCAGTATGAC
    AGGGAGTTTT TGCTGGATTT CCAATTCATG CCAGCGTGTA TTCAGAAACC AGAGGGCCTG
    CCTCCCATCA GTGATGTGGT TCTTGACAAG ATCAATCAAC CAAAATTGCC AATGCGAACT
    CTGGATCCTC GGATTTTGCC TCGAGGACCA GACTTTACTC CAGCCTTTGC AGATTTTGGA
    AGGCAAGCCC CTGGTGGAAG AGGTGTACCT CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316364.1
    CDS196..5121
    Translation

    Target ORF information:

    RefSeq Version XM_015460878.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460878.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCTGCCC
    AGCCAGGTCC CCGAGCACAG CCCTGTGGTC TATGGGACTG TGGAGAGCGC TCATCTTGCT
    GCCAGCACCC CTGTCACTGC AGCGAGCGAC CAGAAGCAAG AAGAGAAGCC AAAACCAGAT
    CCAGTGTTAA AGACTCCTTC CCCAGTCCTT AGGCTAGCCC TCAGTGGAGA GAAGAAAGAA
    CAAGCAGGCC CGACGTCTGA GATTACTGCA GTAGAGTCCA TACCAGAGCT TCCTCTGCCT
    CCATCCCCTA CTGCTATTTC TCCACTTGCT CGAAGTACAA TTACTTCTCC CACCTCTGTT
    GCTCTTAGTA GCCAACCAAT ATTCACCACT GCAATTGATG ACAGATGTGA ACTCTCTTCC
    TCAAAAGAAG ATACAATTCC TATTCCCAGC CCCACATCTT GCACAGAAGC ATCAGATCCT
    TTACCAACAG ATGAAATTGA TGATGATATA TGCAAGAAAT CTTGTAGTGT AGCACCAAAT
    GATATTCCAC TGATTTCTAG TACTAACCTA ATTAATGAAA TGAATGGAGT TAATGAAAAA
    TTACCAGCCA CGGAAAGCAT TGTGGAAATA GTAAAACAGG AGGTGTTACC GCTGACTCTT
    GAATTGGAGA TTTTGGAAAA TCCCCCTGAA GAAATCAAAG TGGAGTGTGT CCCAACTCCC
    ATCACCCCTT CGATAGTTCC CTCCTCTTCT CCGTCTCCTC CAGCTCCTCC AGCTTCTCCT
    TCTCCCACAC CAGTTATCGT TCCTGCTGCT GCCACCAGTG TTAATACTGG TCGTGGTTCC
    ACCGGAGTCC AGAGAGCCTT CGAAGAGGGC GAGAACATAA GAACTTGCCT TAGTGAAGAT
    GCAAAAGAGA TTCAGAACAA AGTAGAGGTA GAAGCAGATG GGCAAACAGA AGAGATTGTG
    GATTCTCAGA ACTTAAGTTC AAGAAAGAGC CCAGTCCCAG CTCAGACAGC TATACCTGCA
    CCAAAGATGT GGAAGAAACC AAAAGACCGG ACCCGAGCCA CTGAAGAGTC GTTAGAGGCA
    GAATCAGAGC CTAAAGCTGA AGAGGAGCTT TCAGGTGACA AAATACTTGA ATCTGAACAG
    GATAAAATGA GCCAAGGGTT TCATCCTGAA AGAGACCCCT CTGACCTAAA AAACGTGAAA
    ACTGTGGAAG AAAATGGAGA AGAAGCTGAG CCTGTACGTA ATGGTGCTGA GAGTATTTCT
    GAGGGTGAAG GAATAGATGC TAATTCAGGC TCCACTGATA GTTCTGGTGA AGGGGTCACA
    TTCCCATTTA AGTCAGAATC CTGGAAGCCT CCTGATTCGG AAGGCAAGAA GCAGTATGAC
    AGGGAGTTTT TGCTGGATTT CCAATTCATG CCAGCGTGTA TTCAGAAACC AGAGGGCCTG
    CCTCCCATCA GTGATGTGGT TCTTGACAAG ATCAATCAAC CAAAATTGCC AATGCGAACT
    CTGGATCCTC GGATTTTGCC TCGAGGACCA GACTTTACTC CAGCCTTTGC AGATTTTGGA
    AGGCAAGCCC CTGGTGGAAG AGGTGTACCT CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88095
    Clone ID Related Accession (Same CDS sequence) XM_015462480.1 , XM_015460884.1 , XM_015460884.2
    Accession Version XM_015462480.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG GGAGGATTCA GACCCATCCA GTTTTTCCAG 
    AGGCCTCAAA TACAGCCTCC CAGAGCCACC ATCCCGAACA GCAGCCCTTC CATTCGACCC
    GGTGCACAGA CACCCACTGC AGTGTATCAG GCCAATCAGC ACATCATGAT GGTTAACCAT
    CTGCCCATGC CGTACCCAGT GCCCCAGGGT CCTCAGTACT GTATACCACA GTACCGTCAC
    AGTGGCCCTC CGTATGTTGG GCCACCACAA CAGTACCCGG TTCAACCACC AGGACCAGGT
    CCTTTTTATC CTGGGCCAGG ACCTGGGGAC TTCCCCAGTG CTTACGGAAC GCCTTTTTAC
    CCAAGTCAGC CAGTGTACCA GTCAGCACCT ATCATAGTGC CTACGCAGCA GCAGCCCCCT
    CCAGCCAAGA GAGAGAAAAA AACAATAAGA ATTCGGGATC CAAACCAGGG AGGTAAAGAC
    ATAACAGAGG AGATTATGTC TGGAGGTGGC AGCCGAAATC CTACTCCACC CATAGGGAGA
    CCCACTTCCA CACCTACTCC TCCTCAGCAG CTGCCCAGCC AGGTCCCCGA GCACAGCCCT
    GTGGTCTATG GGACTGTGGA GAGCGCTCAT CTTGCTGCCA GCACCCCTGT CACTGCAGCG
    AGCGACCAGA AGCAAGAAGA GAAGCCAAAA CCAGATCCAG TGTTAAAGAC TCCTTCCCCA
    GTCCTTAGGC TAGCCCTCAG TGGAGAGAAG AAAGAACAAG CAGGCCCGAC GTCTGAGATT
    ACTGCAGTAG AGTCCATACC AGAGCTTCCT CTGCCTCCAT CCCCTACTGC TATTTCTCCA
    CTTGCTCGAA GTACAATTAC TTCTCCCACC TCTGTTGCTC TTAGTAGCCA ACCAATATTC
    ACCACTGCAA TTGATGACAG ATGTGAACTC TCTTCCTCAA AAGAAGATAC AATTCCTATT
    CCCAGCCCCA CATCTTGCAC AGAAGCATCA GATCCTTTAC CAACAGATGA AATTGATGAT
    GATATATGCA AGAAATCTTG TAGTGTAGCA CCAAATGATA TTCCACTGAT TTCTAGTACT
    AACCTAATTA ATGAAATGAA TGGAGTTAAT GAAAAATTAC CAGCCACGGA AAGCATTGTG
    GAAATAGTAA AACAGGAGGT GTTACCGCTG ACTCTTGAAT TGGAGATTTT GGAAAATCCC
    CCTGAAGAAA TCAAAGTGGA GTGTGTCCCA ACTCCCATCA CCCCTTCGAT AGTTCCCTCC
    TCTTCTCCGT CTCCTCCAGC TCCTCCAGCT TCTCCTTCTC CCACACCAGT TATCGTTCCT
    GCTGCTGCCA CCAGTGTTAA TACTGGTCGT GGTTCCACCG GAGTCCAGAG AGCCTTCGAA
    GAGGGCGAGA ACATAAGAAC TTGCCTTAGT GAAGATGCAA AAGAGATTCA GAACAAAGTA
    GAGGTAGAAG CAGATGGGCA AACAGAAGAG ATTGTGGATT CTCAGAACTT AAGTTCAAGA
    AAGAGCCCAG TCCCAGCTCA GACAGCTATA CCTGCACCAA AGATGTGGAA GAAACCAAAA
    GACCGGACCC GAGCCACTGA AGAGTCGTTA GAGGCAGAAT CAGAGCCTAA AGCTGAAGAG
    GAGCTTTCAG GTGACAAAAT ACTTGAATCT GAACAGGATA AAATGAGCCA AGGGTTTCAT
    CCTGAAAGAG ACCCCTCTGA CCTAAAAAAC GTGAAAACTG TGGAAGAAAA TGGAGAAGAA
    GCTGAGCCTG TACGTAATGG TGCTGAGAGT ATTTCTGAGG GTGAAGGAAT AGATGCTAAT
    TCAGGCTCCA CTGATAGTTC TGGTGAAGGG GTCACATTCC CATTTAAGTC AGAATCCTGG
    AAGCCTCCTG ATTCGGAAGG CAAGAAGCAG TATGACAGGG AGTTTTTGCT GGATTTCCAA
    TTCATGCCAG CGTGTATTCA GAAACCAGAG GGCCTGCCTC CCATCAGTGA TGTGGTTCTT
    GACAAGATCA ATCAACCAAA ATTGCCAATG CGAACTCTGG ATCCTCGGAT TTTGCCTCGA
    GGACCAGACT TTACTCCAGC CTTTGCAGAT TTTGGAAGGC AAGCCCCTGG TGGAAGAGGT
    GTACCTGTTT GCAAAGTGCA GAGCAGGCAT GGATTGCCAA TTCTGGAACA GAGCAAAGCC
    CCAGCTTCCA CTCCACTGGC GATGTCGCAC CCACCCCTGA AGAGCCTGCC TCTAGGGCTG
    TTGAATGTTG GAGCACGGAG ATCTCAGCCT GGCCAGAGGA GAGAGCCCAG GAAGATCATC
    ACGGTCTCCG TGAAAGAAGA CGTGCACCTG AAGAAGGCAG AGAACGCCTG GAAGCCCAGC
    CAGAGGCGGG ATAGCCCAGC CGAGGACCCC GAGAACATCA AGACCCAGGA ACTTTTTAGA
    AAAGTTCGAA GTATCTTAAA TAAATTGACA CCACAGATGT TCAACCAACT GATGAAGCAA
    GTGTCAGGAC TTACTGTTGA CACAGAGGAG CGGCTGAAAG GAGTCATTGA CCTGGTCTTT
    GAGAAGGCTA TTGACGAACC CAGTTTCTCT GTGGCTTACG CAAACATGTG TCGATGTCTA
    GTAACGCTGA AAGTACCCAT GGCAGACAAG CCTGGTAACA CAGTGAACTT CCGGAAGCTA
    CTACTGAACC GTTGCCAGAA GGAATTTGAA AAAGATAAAG CCGATGATGA TGTCTTTGAG
    AAGAAGCAGA AAGAACTCGA GGCTGCCAGT GCTCCAGAGG AGAGGACAAG GCTTCATGAT
    GAACTGGAAG AAGCTAAAGA TAAAGCCCGG CGGAGATCCA TTGGCAACAT CAAGTTCATT
    GGAGAACTCT TTAAACTCAA AATGCTGACT GAAGCCATCA TGCATGACTG TGTGGTGAAG
    CTGCTGAAGA ACCACGATGA AGAATCCCTC GAGTGTCTGT GTCGCCTGCT CACCACAATT
    GGCAAAGACC TGGACTTTGA GAAAGCAAAG CCACGTATGG ACCAGTATTT TAATCAGATG
    GAGAAAATTG TAAAAGAAAG AAAAACCTCA TCTAGGATTC GATTCATGCT TCAGGACGTA
    ATAGATCTAA GGCTGTGTAA TTGGGTATCA CGACGAGCAG ATCAGGGGCC TAAAACTATT
    GAACAGATTC ACAAAGAAGC TAAAATAGAA GAACAGGAAG AGCAGAGGAA GGTCCAACAA
    CTCATGACCA AAGAGAAGAG AAGACCAGGT GTCCAGAGAG TGGATGAAGG TGGGTGGAAC
    ACTGTTCAAG GGGCCAAGAA TAGTCGGGTA TTGGACCCCT CAAAATTTCT GAAAATCACT
    AAGCCCACAA TTGATGAGAA AATTCAGCTG GTACCTAAAG CGCAGCTGGG CAGCTGGGGA
    AAGGGCAGCA GTGGTGGAGC CAAGGCGAGC GAGGCCGATG CCTTAAGATC AAGTGCTTCC
    AGTTTAAACA GATTCTCTGC TTTGCAACCT CCAGCACCTT CAGGGTCTGC GTCATCCACA
    CCTCTAGAGT TTGATTCCCG GCGGACCTTA ACTAGTCGAG GAAGCACGGG CAGGGAGAAG
    AATGACAAGC CCCTCCAGCC TGCAGCAGCT CGGCCGAACA CTTTCATGAG GGGTGGCAGC
    AGTAAAGACC TGTTAGACAA TCAGTCTCAG GAGGAGCAGC GGAGGGAGAT GCTGGAGACC
    GTGAAGCAGC TCACAGGAGG CATGGACGTG GAGAGGAGCA GCGCTGAGGC GGAGCGAAGC
    AGGACCAGGG AGCCAGTGAC AAAACCGGAA ATTCCAGTAA CATCAGCCTC TGACAAGCCC
    GCGTTGTCAG AGGAAGAGAT GGAGAGGAAG TCCAAATCTA TCATTGATGA GTTTCTACAC
    ATTAATGATT TTAAGGAAGC CATGCAGTGT GTGGAAGAGC TGAACGCCCA GGGCCTGCAA
    CATGTGTTCG TGAGGACGGG AGTGGAGTCC ACCCTGGAAA GGAGCCAGAT CACCCGGGAT
    CACATGGGCC AGTTACTGTA TCAGCTGGTG CAGTCAGAAA AGCTCAGCAA ACAGGACTTT
    TTCAGAGGTT TTTCAGAAAC ATTGGAATTG GCAGATGACA TGGCCATTGA TATTCCCCAT
    ATTTGGTTGT ACCTTGCTGA ACTGGTGACT CCCATGTTAA AAGAAGGCGG AATCTCCATG
    AGACAACTCA TCACAGAATT TTGCAAACCT TTACTTCCTG TTGGAAGAGC TGGAATCTTG
    ATTTCTGAAA TATTGCACCT TCTATGCAAA CAAATGAGCC ATAAGAAAGT GGGAGCCCTA
    TGGAGGGAGG CTGACCTCAG CTGGAAGGAC TTTTTACCAG AAGGGGAAGA TGTACATAAT
    TTTCTCTTGG AGCAGAAGTT GGACTTTTTA GAGTCTGACA GTTCCTGTTC CTCCGAAGCA
    CTTTTGAAGA AAGAACTCTC TGCCGAAGAG CTGTATAAGC GACTCGAGAA ACTCATTATT
    GAGGACAAGG CAAATGATGA ACAGATCTTT GACTGGGTAG AGGCTAATCT AGATGAAAGC
    CAGATGAGTT CACCTACATT CCTTAGAGCT TTAATGACAG CCGTCTGTAA AGCAGCTATT
    ATAGCCGACT GTTCGACCTT CCGAGTGGAC ACTGCTGTTA TCAAGCAGAG AGTGCCGATC
    TTACTCAAGT ACCTAGACTC AGATACAGAG AAGGAACTGC AAGCACTTTA TGCACTACAA
    GCATCAATAG TAAAACTTGA TCAACCTGCC AATTTGCTAC GGATGTTTTT TGATTGCCTG
    TATGACGAGG AGGTGATCTC TGAGGATGCC TTCTACAAGT GGGAGAGCAG CAAGGACCCG
    GCAGAGCAGA ATGGGAAGGG TGTGGCCCTG AAGTCTGTCA CGGCGTTCTT CACATGGCTG
    CGGGAAGCAG AAGAGGAGTC TGAGGATAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317966.1
    CDS162..5054
    Translation

    Target ORF information:

    RefSeq Version XM_015462480.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462480.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG GGAGGATTCA GACCCATCCA GTTTTTCCAG 
    AGGCCTCAAA TACAGCCTCC CAGAGCCACC ATCCCGAACA GCAGCCCTTC CATTCGACCC
    GGTGCACAGA CACCCACTGC AGTGTATCAG GCCAATCAGC ACATCATGAT GGTTAACCAT
    CTGCCCATGC CGTACCCAGT GCCCCAGGGT CCTCAGTACT GTATACCACA GTACCGTCAC
    AGTGGCCCTC CGTATGTTGG GCCACCACAA CAGTACCCGG TTCAACCACC AGGACCAGGT
    CCTTTTTATC CTGGGCCAGG ACCTGGGGAC TTCCCCAGTG CTTACGGAAC GCCTTTTTAC
    CCAAGTCAGC CAGTGTACCA GTCAGCACCT ATCATAGTGC CTACGCAGCA GCAGCCCCCT
    CCAGCCAAGA GAGAGAAAAA AACAATAAGA ATTCGGGATC CAAACCAGGG AGGTAAAGAC
    ATAACAGAGG AGATTATGTC TGGAGGTGGC AGCCGAAATC CTACTCCACC CATAGGGAGA
    CCCACTTCCA CACCTACTCC TCCTCAGCAG CTGCCCAGCC AGGTCCCCGA GCACAGCCCT
    GTGGTCTATG GGACTGTGGA GAGCGCTCAT CTTGCTGCCA GCACCCCTGT CACTGCAGCG
    AGCGACCAGA AGCAAGAAGA GAAGCCAAAA CCAGATCCAG TGTTAAAGAC TCCTTCCCCA
    GTCCTTAGGC TAGCCCTCAG TGGAGAGAAG AAAGAACAAG CAGGCCCGAC GTCTGAGATT
    ACTGCAGTAG AGTCCATACC AGAGCTTCCT CTGCCTCCAT CCCCTACTGC TATTTCTCCA
    CTTGCTCGAA GTACAATTAC TTCTCCCACC TCTGTTGCTC TTAGTAGCCA ACCAATATTC
    ACCACTGCAA TTGATGACAG ATGTGAACTC TCTTCCTCAA AAGAAGATAC AATTCCTATT
    CCCAGCCCCA CATCTTGCAC AGAAGCATCA GATCCTTTAC CAACAGATGA AATTGATGAT
    GATATATGCA AGAAATCTTG TAGTGTAGCA CCAAATGATA TTCCACTGAT TTCTAGTACT
    AACCTAATTA ATGAAATGAA TGGAGTTAAT GAAAAATTAC CAGCCACGGA AAGCATTGTG
    GAAATAGTAA AACAGGAGGT GTTACCGCTG ACTCTTGAAT TGGAGATTTT GGAAAATCCC
    CCTGAAGAAA TCAAAGTGGA GTGTGTCCCA ACTCCCATCA CCCCTTCGAT AGTTCCCTCC
    TCTTCTCCGT CTCCTCCAGC TCCTCCAGCT TCTCCTTCTC CCACACCAGT TATCGTTCCT
    GCTGCTGCCA CCAGTGTTAA TACTGGTCGT GGTTCCACCG GAGTCCAGAG AGCCTTCGAA
    GAGGGCGAGA ACATAAGAAC TTGCCTTAGT GAAGATGCAA AAGAGATTCA GAACAAAGTA
    GAGGTAGAAG CAGATGGGCA AACAGAAGAG ATTGTGGATT CTCAGAACTT AAGTTCAAGA
    AAGAGCCCAG TCCCAGCTCA GACAGCTATA CCTGCACCAA AGATGTGGAA GAAACCAAAA
    GACCGGACCC GAGCCACTGA AGAGTCGTTA GAGGCAGAAT CAGAGCCTAA AGCTGAAGAG
    GAGCTTTCAG GTGACAAAAT ACTTGAATCT GAACAGGATA AAATGAGCCA AGGGTTTCAT
    CCTGAAAGAG ACCCCTCTGA CCTAAAAAAC GTGAAAACTG TGGAAGAAAA TGGAGAAGAA
    GCTGAGCCTG TACGTAATGG TGCTGAGAGT ATTTCTGAGG GTGAAGGAAT AGATGCTAAT
    TCAGGCTCCA CTGATAGTTC TGGTGAAGGG GTCACATTCC CATTTAAGTC AGAATCCTGG
    AAGCCTCCTG ATTCGGAAGG CAAGAAGCAG TATGACAGGG AGTTTTTGCT GGATTTCCAA
    TTCATGCCAG CGTGTATTCA GAAACCAGAG GGCCTGCCTC CCATCAGTGA TGTGGTTCTT
    GACAAGATCA ATCAACCAAA ATTGCCAATG CGAACTCTGG ATCCTCGGAT TTTGCCTCGA
    GGACCAGACT TTACTCCAGC CTTTGCAGAT TTTGGAAGGC AAGCCCCTGG TGGAAGAGGT
    GTACCTGTTT GCAAAGTGCA GAGCAGGCAT GGATTGCCAA TTCTGGAACA GAGCAAAGCC
    CCAGCTTCCA CTCCACTGGC GATGTCGCAC CCACCCCTGA AGAGCCTGCC TCTAGGGCTG
    TTGAATGTTG GAGCACGGAG ATCTCAGCCT GGCCAGAGGA GAGAGCCCAG GAAGATCATC
    ACGGTCTCCG TGAAAGAAGA CGTGCACCTG AAGAAGGCAG AGAACGCCTG GAAGCCCAGC
    CAGAGGCGGG ATAGCCCAGC CGAGGACCCC GAGAACATCA AGACCCAGGA ACTTTTTAGA
    AAAGTTCGAA GTATCTTAAA TAAATTGACA CCACAGATGT TCAACCAACT GATGAAGCAA
    GTGTCAGGAC TTACTGTTGA CACAGAGGAG CGGCTGAAAG GAGTCATTGA CCTGGTCTTT
    GAGAAGGCTA TTGACGAACC CAGTTTCTCT GTGGCTTACG CAAACATGTG TCGATGTCTA
    GTAACGCTGA AAGTACCCAT GGCAGACAAG CCTGGTAACA CAGTGAACTT CCGGAAGCTA
    CTACTGAACC GTTGCCAGAA GGAATTTGAA AAAGATAAAG CCGATGATGA TGTCTTTGAG
    AAGAAGCAGA AAGAACTCGA GGCTGCCAGT GCTCCAGAGG AGAGGACAAG GCTTCATGAT
    GAACTGGAAG AAGCTAAAGA TAAAGCCCGG CGGAGATCCA TTGGCAACAT CAAGTTCATT
    GGAGAACTCT TTAAACTCAA AATGCTGACT GAAGCCATCA TGCATGACTG TGTGGTGAAG
    CTGCTGAAGA ACCACGATGA AGAATCCCTC GAGTGTCTGT GTCGCCTGCT CACCACAATT
    GGCAAAGACC TGGACTTTGA GAAAGCAAAG CCACGTATGG ACCAGTATTT TAATCAGATG
    GAGAAAATTG TAAAAGAAAG AAAAACCTCA TCTAGGATTC GATTCATGCT TCAGGACGTA
    ATAGATCTAA GGCTGTGTAA TTGGGTATCA CGACGAGCAG ATCAGGGGCC TAAAACTATT
    GAACAGATTC ACAAAGAAGC TAAAATAGAA GAACAGGAAG AGCAGAGGAA GGTCCAACAA
    CTCATGACCA AAGAGAAGAG AAGACCAGGT GTCCAGAGAG TGGATGAAGG TGGGTGGAAC
    ACTGTTCAAG GGGCCAAGAA TAGTCGGGTA TTGGACCCCT CAAAATTTCT GAAAATCACT
    AAGCCCACAA TTGATGAGAA AATTCAGCTG GTACCTAAAG CGCAGCTGGG CAGCTGGGGA
    AAGGGCAGCA GTGGTGGAGC CAAGGCGAGC GAGGCCGATG CCTTAAGATC AAGTGCTTCC
    AGTTTAAACA GATTCTCTGC TTTGCAACCT CCAGCACCTT CAGGGTCTGC GTCATCCACA
    CCTCTAGAGT TTGATTCCCG GCGGACCTTA ACTAGTCGAG GAAGCACGGG CAGGGAGAAG
    AATGACAAGC CCCTCCAGCC TGCAGCAGCT CGGCCGAACA CTTTCATGAG GGGTGGCAGC
    AGTAAAGACC TGTTAGACAA TCAGTCTCAG GAGGAGCAGC GGAGGGAGAT GCTGGAGACC
    GTGAAGCAGC TCACAGGAGG CATGGACGTG GAGAGGAGCA GCGCTGAGGC GGAGCGAAGC
    AGGACCAGGG AGCCAGTGAC AAAACCGGAA ATTCCAGTAA CATCAGCCTC TGACAAGCCC
    GCGTTGTCAG AGGAAGAGAT GGAGAGGAAG TCCAAATCTA TCATTGATGA GTTTCTACAC
    ATTAATGATT TTAAGGAAGC CATGCAGTGT GTGGAAGAGC TGAACGCCCA GGGCCTGCAA
    CATGTGTTCG TGAGGACGGG AGTGGAGTCC ACCCTGGAAA GGAGCCAGAT CACCCGGGAT
    CACATGGGCC AGTTACTGTA TCAGCTGGTG CAGTCAGAAA AGCTCAGCAA ACAGGACTTT
    TTCAGAGGTT TTTCAGAAAC ATTGGAATTG GCAGATGACA TGGCCATTGA TATTCCCCAT
    ATTTGGTTGT ACCTTGCTGA ACTGGTGACT CCCATGTTAA AAGAAGGCGG AATCTCCATG
    AGACAACTCA TCACAGAATT TTGCAAACCT TTACTTCCTG TTGGAAGAGC TGGAATCTTG
    ATTTCTGAAA TATTGCACCT TCTATGCAAA CAAATGAGCC ATAAGAAAGT GGGAGCCCTA
    TGGAGGGAGG CTGACCTCAG CTGGAAGGAC TTTTTACCAG AAGGGGAAGA TGTACATAAT
    TTTCTCTTGG AGCAGAAGTT GGACTTTTTA GAGTCTGACA GTTCCTGTTC CTCCGAAGCA
    CTTTTGAAGA AAGAACTCTC TGCCGAAGAG CTGTATAAGC GACTCGAGAA ACTCATTATT
    GAGGACAAGG CAAATGATGA ACAGATCTTT GACTGGGTAG AGGCTAATCT AGATGAAAGC
    CAGATGAGTT CACCTACATT CCTTAGAGCT TTAATGACAG CCGTCTGTAA AGCAGCTATT
    ATAGCCGACT GTTCGACCTT CCGAGTGGAC ACTGCTGTTA TCAAGCAGAG AGTGCCGATC
    TTACTCAAGT ACCTAGACTC AGATACAGAG AAGGAACTGC AAGCACTTTA TGCACTACAA
    GCATCAATAG TAAAACTTGA TCAACCTGCC AATTTGCTAC GGATGTTTTT TGATTGCCTG
    TATGACGAGG AGGTGATCTC TGAGGATGCC TTCTACAAGT GGGAGAGCAG CAAGGACCCG
    GCAGAGCAGA ATGGGAAGGG TGTGGCCCTG AAGTCTGTCA CGGCGTTCTT CACATGGCTG
    CGGGAAGCAG AAGAGGAGTC TGAGGATAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88095
    Clone ID Related Accession (Same CDS sequence) XM_015462480.1 , XM_015460884.1 , XM_015460884.2
    Accession Version XM_015460884.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG GGAGGATTCA GACCCATCCA GTTTTTCCAG 
    AGGCCTCAAA TACAGCCTCC CAGAGCCACC ATCCCGAACA GCAGCCCTTC CATTCGACCC
    GGTGCACAGA CACCCACTGC AGTGTATCAG GCCAATCAGC ACATCATGAT GGTTAACCAT
    CTGCCCATGC CGTACCCAGT GCCCCAGGGT CCTCAGTACT GTATACCACA GTACCGTCAC
    AGTGGCCCTC CGTATGTTGG GCCACCACAA CAGTACCCGG TTCAACCACC AGGACCAGGT
    CCTTTTTATC CTGGGCCAGG ACCTGGGGAC TTCCCCAGTG CTTACGGAAC GCCTTTTTAC
    CCAAGTCAGC CAGTGTACCA GTCAGCACCT ATCATAGTGC CTACGCAGCA GCAGCCCCCT
    CCAGCCAAGA GAGAGAAAAA AACAATAAGA ATTCGGGATC CAAACCAGGG AGGTAAAGAC
    ATAACAGAGG AGATTATGTC TGGAGGTGGC AGCCGAAATC CTACTCCACC CATAGGGAGA
    CCCACTTCCA CACCTACTCC TCCTCAGCAG CTGCCCAGCC AGGTCCCCGA GCACAGCCCT
    GTGGTCTATG GGACTGTGGA GAGCGCTCAT CTTGCTGCCA GCACCCCTGT CACTGCAGCG
    AGCGACCAGA AGCAAGAAGA GAAGCCAAAA CCAGATCCAG TGTTAAAGAC TCCTTCCCCA
    GTCCTTAGGC TAGCCCTCAG TGGAGAGAAG AAAGAACAAG CAGGCCCGAC GTCTGAGATT
    ACTGCAGTAG AGTCCATACC AGAGCTTCCT CTGCCTCCAT CCCCTACTGC TATTTCTCCA
    CTTGCTCGAA GTACAATTAC TTCTCCCACC TCTGTTGCTC TTAGTAGCCA ACCAATATTC
    ACCACTGCAA TTGATGACAG ATGTGAACTC TCTTCCTCAA AAGAAGATAC AATTCCTATT
    CCCAGCCCCA CATCTTGCAC AGAAGCATCA GATCCTTTAC CAACAGATGA AATTGATGAT
    GATATATGCA AGAAATCTTG TAGTGTAGCA CCAAATGATA TTCCACTGAT TTCTAGTACT
    AACCTAATTA ATGAAATGAA TGGAGTTAAT GAAAAATTAC CAGCCACGGA AAGCATTGTG
    GAAATAGTAA AACAGGAGGT GTTACCGCTG ACTCTTGAAT TGGAGATTTT GGAAAATCCC
    CCTGAAGAAA TCAAAGTGGA GTGTGTCCCA ACTCCCATCA CCCCTTCGAT AGTTCCCTCC
    TCTTCTCCGT CTCCTCCAGC TCCTCCAGCT TCTCCTTCTC CCACACCAGT TATCGTTCCT
    GCTGCTGCCA CCAGTGTTAA TACTGGTCGT GGTTCCACCG GAGTCCAGAG AGCCTTCGAA
    GAGGGCGAGA ACATAAGAAC TTGCCTTAGT GAAGATGCAA AAGAGATTCA GAACAAAGTA
    GAGGTAGAAG CAGATGGGCA AACAGAAGAG ATTGTGGATT CTCAGAACTT AAGTTCAAGA
    AAGAGCCCAG TCCCAGCTCA GACAGCTATA CCTGCACCAA AGATGTGGAA GAAACCAAAA
    GACCGGACCC GAGCCACTGA AGAGTCGTTA GAGGCAGAAT CAGAGCCTAA AGCTGAAGAG
    GAGCTTTCAG GTGACAAAAT ACTTGAATCT GAACAGGATA AAATGAGCCA AGGGTTTCAT
    CCTGAAAGAG ACCCCTCTGA CCTAAAAAAC GTGAAAACTG TGGAAGAAAA TGGAGAAGAA
    GCTGAGCCTG TACGTAATGG TGCTGAGAGT ATTTCTGAGG GTGAAGGAAT AGATGCTAAT
    TCAGGCTCCA CTGATAGTTC TGGTGAAGGG GTCACATTCC CATTTAAGTC AGAATCCTGG
    AAGCCTCCTG ATTCGGAAGG CAAGAAGCAG TATGACAGGG AGTTTTTGCT GGATTTCCAA
    TTCATGCCAG CGTGTATTCA GAAACCAGAG GGCCTGCCTC CCATCAGTGA TGTGGTTCTT
    GACAAGATCA ATCAACCAAA ATTGCCAATG CGAACTCTGG ATCCTCGGAT TTTGCCTCGA
    GGACCAGACT TTACTCCAGC CTTTGCAGAT TTTGGAAGGC AAGCCCCTGG TGGAAGAGGT
    GTACCTGTTT GCAAAGTGCA GAGCAGGCAT GGATTGCCAA TTCTGGAACA GAGCAAAGCC
    CCAGCTTCCA CTCCACTGGC GATGTCGCAC CCACCCCTGA AGAGCCTGCC TCTAGGGCTG
    TTGAATGTTG GAGCACGGAG ATCTCAGCCT GGCCAGAGGA GAGAGCCCAG GAAGATCATC
    ACGGTCTCCG TGAAAGAAGA CGTGCACCTG AAGAAGGCAG AGAACGCCTG GAAGCCCAGC
    CAGAGGCGGG ATAGCCCAGC CGAGGACCCC GAGAACATCA AGACCCAGGA ACTTTTTAGA
    AAAGTTCGAA GTATCTTAAA TAAATTGACA CCACAGATGT TCAACCAACT GATGAAGCAA
    GTGTCAGGAC TTACTGTTGA CACAGAGGAG CGGCTGAAAG GAGTCATTGA CCTGGTCTTT
    GAGAAGGCTA TTGACGAACC CAGTTTCTCT GTGGCTTACG CAAACATGTG TCGATGTCTA
    GTAACGCTGA AAGTACCCAT GGCAGACAAG CCTGGTAACA CAGTGAACTT CCGGAAGCTA
    CTACTGAACC GTTGCCAGAA GGAATTTGAA AAAGATAAAG CCGATGATGA TGTCTTTGAG
    AAGAAGCAGA AAGAACTCGA GGCTGCCAGT GCTCCAGAGG AGAGGACAAG GCTTCATGAT
    GAACTGGAAG AAGCTAAAGA TAAAGCCCGG CGGAGATCCA TTGGCAACAT CAAGTTCATT
    GGAGAACTCT TTAAACTCAA AATGCTGACT GAAGCCATCA TGCATGACTG TGTGGTGAAG
    CTGCTGAAGA ACCACGATGA AGAATCCCTC GAGTGTCTGT GTCGCCTGCT CACCACAATT
    GGCAAAGACC TGGACTTTGA GAAAGCAAAG CCACGTATGG ACCAGTATTT TAATCAGATG
    GAGAAAATTG TAAAAGAAAG AAAAACCTCA TCTAGGATTC GATTCATGCT TCAGGACGTA
    ATAGATCTAA GGCTGTGTAA TTGGGTATCA CGACGAGCAG ATCAGGGGCC TAAAACTATT
    GAACAGATTC ACAAAGAAGC TAAAATAGAA GAACAGGAAG AGCAGAGGAA GGTCCAACAA
    CTCATGACCA AAGAGAAGAG AAGACCAGGT GTCCAGAGAG TGGATGAAGG TGGGTGGAAC
    ACTGTTCAAG GGGCCAAGAA TAGTCGGGTA TTGGACCCCT CAAAATTTCT GAAAATCACT
    AAGCCCACAA TTGATGAGAA AATTCAGCTG GTACCTAAAG CGCAGCTGGG CAGCTGGGGA
    AAGGGCAGCA GTGGTGGAGC CAAGGCGAGC GAGGCCGATG CCTTAAGATC AAGTGCTTCC
    AGTTTAAACA GATTCTCTGC TTTGCAACCT CCAGCACCTT CAGGGTCTGC GTCATCCACA
    CCTCTAGAGT TTGATTCCCG GCGGACCTTA ACTAGTCGAG GAAGCACGGG CAGGGAGAAG
    AATGACAAGC CCCTCCAGCC TGCAGCAGCT CGGCCGAACA CTTTCATGAG GGGTGGCAGC
    AGTAAAGACC TGTTAGACAA TCAGTCTCAG GAGGAGCAGC GGAGGGAGAT GCTGGAGACC
    GTGAAGCAGC TCACAGGAGG CATGGACGTG GAGAGGAGCA GCGCTGAGGC GGAGCGAAGC
    AGGACCAGGG AGCCAGTGAC AAAACCGGAA ATTCCAGTAA CATCAGCCTC TGACAAGCCC
    GCGTTGTCAG AGGAAGAGAT GGAGAGGAAG TCCAAATCTA TCATTGATGA GTTTCTACAC
    ATTAATGATT TTAAGGAAGC CATGCAGTGT GTGGAAGAGC TGAACGCCCA GGGCCTGCAA
    CATGTGTTCG TGAGGACGGG AGTGGAGTCC ACCCTGGAAA GGAGCCAGAT CACCCGGGAT
    CACATGGGCC AGTTACTGTA TCAGCTGGTG CAGTCAGAAA AGCTCAGCAA ACAGGACTTT
    TTCAGAGGTT TTTCAGAAAC ATTGGAATTG GCAGATGACA TGGCCATTGA TATTCCCCAT
    ATTTGGTTGT ACCTTGCTGA ACTGGTGACT CCCATGTTAA AAGAAGGCGG AATCTCCATG
    AGACAACTCA TCACAGAATT TTGCAAACCT TTACTTCCTG TTGGAAGAGC TGGAATCTTG
    ATTTCTGAAA TATTGCACCT TCTATGCAAA CAAATGAGCC ATAAGAAAGT GGGAGCCCTA
    TGGAGGGAGG CTGACCTCAG CTGGAAGGAC TTTTTACCAG AAGGGGAAGA TGTACATAAT
    TTTCTCTTGG AGCAGAAGTT GGACTTTTTA GAGTCTGACA GTTCCTGTTC CTCCGAAGCA
    CTTTTGAAGA AAGAACTCTC TGCCGAAGAG CTGTATAAGC GACTCGAGAA ACTCATTATT
    GAGGACAAGG CAAATGATGA ACAGATCTTT GACTGGGTAG AGGCTAATCT AGATGAAAGC
    CAGATGAGTT CACCTACATT CCTTAGAGCT TTAATGACAG CCGTCTGTAA AGCAGCTATT
    ATAGCCGACT GTTCGACCTT CCGAGTGGAC ACTGCTGTTA TCAAGCAGAG AGTGCCGATC
    TTACTCAAGT ACCTAGACTC AGATACAGAG AAGGAACTGC AAGCACTTTA TGCACTACAA
    GCATCAATAG TAAAACTTGA TCAACCTGCC AATTTGCTAC GGATGTTTTT TGATTGCCTG
    TATGACGAGG AGGTGATCTC TGAGGATGCC TTCTACAAGT GGGAGAGCAG CAAGGACCCG
    GCAGAGCAGA ATGGGAAGGG TGTGGCCCTG AAGTCTGTCA CGGCGTTCTT CACATGGCTG
    CGGGAAGCAG AAGAGGAGTC TGAGGATAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316370.1
    CDS162..5054
    Translation

    Target ORF information:

    RefSeq Version XM_015460884.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG GGAGGATTCA GACCCATCCA GTTTTTCCAG 
    AGGCCTCAAA TACAGCCTCC CAGAGCCACC ATCCCGAACA GCAGCCCTTC CATTCGACCC
    GGTGCACAGA CACCCACTGC AGTGTATCAG GCCAATCAGC ACATCATGAT GGTTAACCAT
    CTGCCCATGC CGTACCCAGT GCCCCAGGGT CCTCAGTACT GTATACCACA GTACCGTCAC
    AGTGGCCCTC CGTATGTTGG GCCACCACAA CAGTACCCGG TTCAACCACC AGGACCAGGT
    CCTTTTTATC CTGGGCCAGG ACCTGGGGAC TTCCCCAGTG CTTACGGAAC GCCTTTTTAC
    CCAAGTCAGC CAGTGTACCA GTCAGCACCT ATCATAGTGC CTACGCAGCA GCAGCCCCCT
    CCAGCCAAGA GAGAGAAAAA AACAATAAGA ATTCGGGATC CAAACCAGGG AGGTAAAGAC
    ATAACAGAGG AGATTATGTC TGGAGGTGGC AGCCGAAATC CTACTCCACC CATAGGGAGA
    CCCACTTCCA CACCTACTCC TCCTCAGCAG CTGCCCAGCC AGGTCCCCGA GCACAGCCCT
    GTGGTCTATG GGACTGTGGA GAGCGCTCAT CTTGCTGCCA GCACCCCTGT CACTGCAGCG
    AGCGACCAGA AGCAAGAAGA GAAGCCAAAA CCAGATCCAG TGTTAAAGAC TCCTTCCCCA
    GTCCTTAGGC TAGCCCTCAG TGGAGAGAAG AAAGAACAAG CAGGCCCGAC GTCTGAGATT
    ACTGCAGTAG AGTCCATACC AGAGCTTCCT CTGCCTCCAT CCCCTACTGC TATTTCTCCA
    CTTGCTCGAA GTACAATTAC TTCTCCCACC TCTGTTGCTC TTAGTAGCCA ACCAATATTC
    ACCACTGCAA TTGATGACAG ATGTGAACTC TCTTCCTCAA AAGAAGATAC AATTCCTATT
    CCCAGCCCCA CATCTTGCAC AGAAGCATCA GATCCTTTAC CAACAGATGA AATTGATGAT
    GATATATGCA AGAAATCTTG TAGTGTAGCA CCAAATGATA TTCCACTGAT TTCTAGTACT
    AACCTAATTA ATGAAATGAA TGGAGTTAAT GAAAAATTAC CAGCCACGGA AAGCATTGTG
    GAAATAGTAA AACAGGAGGT GTTACCGCTG ACTCTTGAAT TGGAGATTTT GGAAAATCCC
    CCTGAAGAAA TCAAAGTGGA GTGTGTCCCA ACTCCCATCA CCCCTTCGAT AGTTCCCTCC
    TCTTCTCCGT CTCCTCCAGC TCCTCCAGCT TCTCCTTCTC CCACACCAGT TATCGTTCCT
    GCTGCTGCCA CCAGTGTTAA TACTGGTCGT GGTTCCACCG GAGTCCAGAG AGCCTTCGAA
    GAGGGCGAGA ACATAAGAAC TTGCCTTAGT GAAGATGCAA AAGAGATTCA GAACAAAGTA
    GAGGTAGAAG CAGATGGGCA AACAGAAGAG ATTGTGGATT CTCAGAACTT AAGTTCAAGA
    AAGAGCCCAG TCCCAGCTCA GACAGCTATA CCTGCACCAA AGATGTGGAA GAAACCAAAA
    GACCGGACCC GAGCCACTGA AGAGTCGTTA GAGGCAGAAT CAGAGCCTAA AGCTGAAGAG
    GAGCTTTCAG GTGACAAAAT ACTTGAATCT GAACAGGATA AAATGAGCCA AGGGTTTCAT
    CCTGAAAGAG ACCCCTCTGA CCTAAAAAAC GTGAAAACTG TGGAAGAAAA TGGAGAAGAA
    GCTGAGCCTG TACGTAATGG TGCTGAGAGT ATTTCTGAGG GTGAAGGAAT AGATGCTAAT
    TCAGGCTCCA CTGATAGTTC TGGTGAAGGG GTCACATTCC CATTTAAGTC AGAATCCTGG
    AAGCCTCCTG ATTCGGAAGG CAAGAAGCAG TATGACAGGG AGTTTTTGCT GGATTTCCAA
    TTCATGCCAG CGTGTATTCA GAAACCAGAG GGCCTGCCTC CCATCAGTGA TGTGGTTCTT
    GACAAGATCA ATCAACCAAA ATTGCCAATG CGAACTCTGG ATCCTCGGAT TTTGCCTCGA
    GGACCAGACT TTACTCCAGC CTTTGCAGAT TTTGGAAGGC AAGCCCCTGG TGGAAGAGGT
    GTACCTGTTT GCAAAGTGCA GAGCAGGCAT GGATTGCCAA TTCTGGAACA GAGCAAAGCC
    CCAGCTTCCA CTCCACTGGC GATGTCGCAC CCACCCCTGA AGAGCCTGCC TCTAGGGCTG
    TTGAATGTTG GAGCACGGAG ATCTCAGCCT GGCCAGAGGA GAGAGCCCAG GAAGATCATC
    ACGGTCTCCG TGAAAGAAGA CGTGCACCTG AAGAAGGCAG AGAACGCCTG GAAGCCCAGC
    CAGAGGCGGG ATAGCCCAGC CGAGGACCCC GAGAACATCA AGACCCAGGA ACTTTTTAGA
    AAAGTTCGAA GTATCTTAAA TAAATTGACA CCACAGATGT TCAACCAACT GATGAAGCAA
    GTGTCAGGAC TTACTGTTGA CACAGAGGAG CGGCTGAAAG GAGTCATTGA CCTGGTCTTT
    GAGAAGGCTA TTGACGAACC CAGTTTCTCT GTGGCTTACG CAAACATGTG TCGATGTCTA
    GTAACGCTGA AAGTACCCAT GGCAGACAAG CCTGGTAACA CAGTGAACTT CCGGAAGCTA
    CTACTGAACC GTTGCCAGAA GGAATTTGAA AAAGATAAAG CCGATGATGA TGTCTTTGAG
    AAGAAGCAGA AAGAACTCGA GGCTGCCAGT GCTCCAGAGG AGAGGACAAG GCTTCATGAT
    GAACTGGAAG AAGCTAAAGA TAAAGCCCGG CGGAGATCCA TTGGCAACAT CAAGTTCATT
    GGAGAACTCT TTAAACTCAA AATGCTGACT GAAGCCATCA TGCATGACTG TGTGGTGAAG
    CTGCTGAAGA ACCACGATGA AGAATCCCTC GAGTGTCTGT GTCGCCTGCT CACCACAATT
    GGCAAAGACC TGGACTTTGA GAAAGCAAAG CCACGTATGG ACCAGTATTT TAATCAGATG
    GAGAAAATTG TAAAAGAAAG AAAAACCTCA TCTAGGATTC GATTCATGCT TCAGGACGTA
    ATAGATCTAA GGCTGTGTAA TTGGGTATCA CGACGAGCAG ATCAGGGGCC TAAAACTATT
    GAACAGATTC ACAAAGAAGC TAAAATAGAA GAACAGGAAG AGCAGAGGAA GGTCCAACAA
    CTCATGACCA AAGAGAAGAG AAGACCAGGT GTCCAGAGAG TGGATGAAGG TGGGTGGAAC
    ACTGTTCAAG GGGCCAAGAA TAGTCGGGTA TTGGACCCCT CAAAATTTCT GAAAATCACT
    AAGCCCACAA TTGATGAGAA AATTCAGCTG GTACCTAAAG CGCAGCTGGG CAGCTGGGGA
    AAGGGCAGCA GTGGTGGAGC CAAGGCGAGC GAGGCCGATG CCTTAAGATC AAGTGCTTCC
    AGTTTAAACA GATTCTCTGC TTTGCAACCT CCAGCACCTT CAGGGTCTGC GTCATCCACA
    CCTCTAGAGT TTGATTCCCG GCGGACCTTA ACTAGTCGAG GAAGCACGGG CAGGGAGAAG
    AATGACAAGC CCCTCCAGCC TGCAGCAGCT CGGCCGAACA CTTTCATGAG GGGTGGCAGC
    AGTAAAGACC TGTTAGACAA TCAGTCTCAG GAGGAGCAGC GGAGGGAGAT GCTGGAGACC
    GTGAAGCAGC TCACAGGAGG CATGGACGTG GAGAGGAGCA GCGCTGAGGC GGAGCGAAGC
    AGGACCAGGG AGCCAGTGAC AAAACCGGAA ATTCCAGTAA CATCAGCCTC TGACAAGCCC
    GCGTTGTCAG AGGAAGAGAT GGAGAGGAAG TCCAAATCTA TCATTGATGA GTTTCTACAC
    ATTAATGATT TTAAGGAAGC CATGCAGTGT GTGGAAGAGC TGAACGCCCA GGGCCTGCAA
    CATGTGTTCG TGAGGACGGG AGTGGAGTCC ACCCTGGAAA GGAGCCAGAT CACCCGGGAT
    CACATGGGCC AGTTACTGTA TCAGCTGGTG CAGTCAGAAA AGCTCAGCAA ACAGGACTTT
    TTCAGAGGTT TTTCAGAAAC ATTGGAATTG GCAGATGACA TGGCCATTGA TATTCCCCAT
    ATTTGGTTGT ACCTTGCTGA ACTGGTGACT CCCATGTTAA AAGAAGGCGG AATCTCCATG
    AGACAACTCA TCACAGAATT TTGCAAACCT TTACTTCCTG TTGGAAGAGC TGGAATCTTG
    ATTTCTGAAA TATTGCACCT TCTATGCAAA CAAATGAGCC ATAAGAAAGT GGGAGCCCTA
    TGGAGGGAGG CTGACCTCAG CTGGAAGGAC TTTTTACCAG AAGGGGAAGA TGTACATAAT
    TTTCTCTTGG AGCAGAAGTT GGACTTTTTA GAGTCTGACA GTTCCTGTTC CTCCGAAGCA
    CTTTTGAAGA AAGAACTCTC TGCCGAAGAG CTGTATAAGC GACTCGAGAA ACTCATTATT
    GAGGACAAGG CAAATGATGA ACAGATCTTT GACTGGGTAG AGGCTAATCT AGATGAAAGC
    CAGATGAGTT CACCTACATT CCTTAGAGCT TTAATGACAG CCGTCTGTAA AGCAGCTATT
    ATAGCCGACT GTTCGACCTT CCGAGTGGAC ACTGCTGTTA TCAAGCAGAG AGTGCCGATC
    TTACTCAAGT ACCTAGACTC AGATACAGAG AAGGAACTGC AAGCACTTTA TGCACTACAA
    GCATCAATAG TAAAACTTGA TCAACCTGCC AATTTGCTAC GGATGTTTTT TGATTGCCTG
    TATGACGAGG AGGTGATCTC TGAGGATGCC TTCTACAAGT GGGAGAGCAG CAAGGACCCG
    GCAGAGCAGA ATGGGAAGGG TGTGGCCCTG AAGTCTGTCA CGGCGTTCTT CACATGGCTG
    CGGGAAGCAG AAGAGGAGTC TGAGGATAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88095
    Clone ID Related Accession (Same CDS sequence) XM_015462480.1 , XM_015460884.1 , XM_015460884.2
    Accession Version XM_015460884.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460884.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG GGAGGATTCA GACCCATCCA GTTTTTCCAG 
    AGGCCTCAAA TACAGCCTCC CAGAGCCACC ATCCCGAACA GCAGCCCTTC CATTCGACCC
    GGTGCACAGA CACCCACTGC AGTGTATCAG GCCAATCAGC ACATCATGAT GGTTAACCAT
    CTGCCCATGC CGTACCCAGT GCCCCAGGGT CCTCAGTACT GTATACCACA GTACCGTCAC
    AGTGGCCCTC CGTATGTTGG GCCACCACAA CAGTACCCGG TTCAACCACC AGGACCAGGT
    CCTTTTTATC CTGGGCCAGG ACCTGGGGAC TTCCCCAGTG CTTACGGAAC GCCTTTTTAC
    CCAAGTCAGC CAGTGTACCA GTCAGCACCT ATCATAGTGC CTACGCAGCA GCAGCCCCCT
    CCAGCCAAGA GAGAGAAAAA AACAATAAGA ATTCGGGATC CAAACCAGGG AGGTAAAGAC
    ATAACAGAGG AGATTATGTC TGGAGGTGGC AGCCGAAATC CTACTCCACC CATAGGGAGA
    CCCACTTCCA CACCTACTCC TCCTCAGCAG CTGCCCAGCC AGGTCCCCGA GCACAGCCCT
    GTGGTCTATG GGACTGTGGA GAGCGCTCAT CTTGCTGCCA GCACCCCTGT CACTGCAGCG
    AGCGACCAGA AGCAAGAAGA GAAGCCAAAA CCAGATCCAG TGTTAAAGAC TCCTTCCCCA
    GTCCTTAGGC TAGCCCTCAG TGGAGAGAAG AAAGAACAAG CAGGCCCGAC GTCTGAGATT
    ACTGCAGTAG AGTCCATACC AGAGCTTCCT CTGCCTCCAT CCCCTACTGC TATTTCTCCA
    CTTGCTCGAA GTACAATTAC TTCTCCCACC TCTGTTGCTC TTAGTAGCCA ACCAATATTC
    ACCACTGCAA TTGATGACAG ATGTGAACTC TCTTCCTCAA AAGAAGATAC AATTCCTATT
    CCCAGCCCCA CATCTTGCAC AGAAGCATCA GATCCTTTAC CAACAGATGA AATTGATGAT
    GATATATGCA AGAAATCTTG TAGTGTAGCA CCAAATGATA TTCCACTGAT TTCTAGTACT
    AACCTAATTA ATGAAATGAA TGGAGTTAAT GAAAAATTAC CAGCCACGGA AAGCATTGTG
    GAAATAGTAA AACAGGAGGT GTTACCGCTG ACTCTTGAAT TGGAGATTTT GGAAAATCCC
    CCTGAAGAAA TCAAAGTGGA GTGTGTCCCA ACTCCCATCA CCCCTTCGAT AGTTCCCTCC
    TCTTCTCCGT CTCCTCCAGC TCCTCCAGCT TCTCCTTCTC CCACACCAGT TATCGTTCCT
    GCTGCTGCCA CCAGTGTTAA TACTGGTCGT GGTTCCACCG GAGTCCAGAG AGCCTTCGAA
    GAGGGCGAGA ACATAAGAAC TTGCCTTAGT GAAGATGCAA AAGAGATTCA GAACAAAGTA
    GAGGTAGAAG CAGATGGGCA AACAGAAGAG ATTGTGGATT CTCAGAACTT AAGTTCAAGA
    AAGAGCCCAG TCCCAGCTCA GACAGCTATA CCTGCACCAA AGATGTGGAA GAAACCAAAA
    GACCGGACCC GAGCCACTGA AGAGTCGTTA GAGGCAGAAT CAGAGCCTAA AGCTGAAGAG
    GAGCTTTCAG GTGACAAAAT ACTTGAATCT GAACAGGATA AAATGAGCCA AGGGTTTCAT
    CCTGAAAGAG ACCCCTCTGA CCTAAAAAAC GTGAAAACTG TGGAAGAAAA TGGAGAAGAA
    GCTGAGCCTG TACGTAATGG TGCTGAGAGT ATTTCTGAGG GTGAAGGAAT AGATGCTAAT
    TCAGGCTCCA CTGATAGTTC TGGTGAAGGG GTCACATTCC CATTTAAGTC AGAATCCTGG
    AAGCCTCCTG ATTCGGAAGG CAAGAAGCAG TATGACAGGG AGTTTTTGCT GGATTTCCAA
    TTCATGCCAG CGTGTATTCA GAAACCAGAG GGCCTGCCTC CCATCAGTGA TGTGGTTCTT
    GACAAGATCA ATCAACCAAA ATTGCCAATG CGAACTCTGG ATCCTCGGAT TTTGCCTCGA
    GGACCAGACT TTACTCCAGC CTTTGCAGAT TTTGGAAGGC AAGCCCCTGG TGGAAGAGGT
    GTACCTGTTT GCAAAGTGCA GAGCAGGCAT GGATTGCCAA TTCTGGAACA GAGCAAAGCC
    CCAGCTTCCA CTCCACTGGC GATGTCGCAC CCACCCCTGA AGAGCCTGCC TCTAGGGCTG
    TTGAATGTTG GAGCACGGAG ATCTCAGCCT GGCCAGAGGA GAGAGCCCAG GAAGATCATC
    ACGGTCTCCG TGAAAGAAGA CGTGCACCTG AAGAAGGCAG AGAACGCCTG GAAGCCCAGC
    CAGAGGCGGG ATAGCCCAGC CGAGGACCCC GAGAACATCA AGACCCAGGA ACTTTTTAGA
    AAAGTTCGAA GTATCTTAAA TAAATTGACA CCACAGATGT TCAACCAACT GATGAAGCAA
    GTGTCAGGAC TTACTGTTGA CACAGAGGAG CGGCTGAAAG GAGTCATTGA CCTGGTCTTT
    GAGAAGGCTA TTGACGAACC CAGTTTCTCT GTGGCTTACG CAAACATGTG TCGATGTCTA
    GTAACGCTGA AAGTACCCAT GGCAGACAAG CCTGGTAACA CAGTGAACTT CCGGAAGCTA
    CTACTGAACC GTTGCCAGAA GGAATTTGAA AAAGATAAAG CCGATGATGA TGTCTTTGAG
    AAGAAGCAGA AAGAACTCGA GGCTGCCAGT GCTCCAGAGG AGAGGACAAG GCTTCATGAT
    GAACTGGAAG AAGCTAAAGA TAAAGCCCGG CGGAGATCCA TTGGCAACAT CAAGTTCATT
    GGAGAACTCT TTAAACTCAA AATGCTGACT GAAGCCATCA TGCATGACTG TGTGGTGAAG
    CTGCTGAAGA ACCACGATGA AGAATCCCTC GAGTGTCTGT GTCGCCTGCT CACCACAATT
    GGCAAAGACC TGGACTTTGA GAAAGCAAAG CCACGTATGG ACCAGTATTT TAATCAGATG
    GAGAAAATTG TAAAAGAAAG AAAAACCTCA TCTAGGATTC GATTCATGCT TCAGGACGTA
    ATAGATCTAA GGCTGTGTAA TTGGGTATCA CGACGAGCAG ATCAGGGGCC TAAAACTATT
    GAACAGATTC ACAAAGAAGC TAAAATAGAA GAACAGGAAG AGCAGAGGAA GGTCCAACAA
    CTCATGACCA AAGAGAAGAG AAGACCAGGT GTCCAGAGAG TGGATGAAGG TGGGTGGAAC
    ACTGTTCAAG GGGCCAAGAA TAGTCGGGTA TTGGACCCCT CAAAATTTCT GAAAATCACT
    AAGCCCACAA TTGATGAGAA AATTCAGCTG GTACCTAAAG CGCAGCTGGG CAGCTGGGGA
    AAGGGCAGCA GTGGTGGAGC CAAGGCGAGC GAGGCCGATG CCTTAAGATC AAGTGCTTCC
    AGTTTAAACA GATTCTCTGC TTTGCAACCT CCAGCACCTT CAGGGTCTGC GTCATCCACA
    CCTCTAGAGT TTGATTCCCG GCGGACCTTA ACTAGTCGAG GAAGCACGGG CAGGGAGAAG
    AATGACAAGC CCCTCCAGCC TGCAGCAGCT CGGCCGAACA CTTTCATGAG GGGTGGCAGC
    AGTAAAGACC TGTTAGACAA TCAGTCTCAG GAGGAGCAGC GGAGGGAGAT GCTGGAGACC
    GTGAAGCAGC TCACAGGAGG CATGGACGTG GAGAGGAGCA GCGCTGAGGC GGAGCGAAGC
    AGGACCAGGG AGCCAGTGAC AAAACCGGAA ATTCCAGTAA CATCAGCCTC TGACAAGCCC
    GCGTTGTCAG AGGAAGAGAT GGAGAGGAAG TCCAAATCTA TCATTGATGA GTTTCTACAC
    ATTAATGATT TTAAGGAAGC CATGCAGTGT GTGGAAGAGC TGAACGCCCA GGGCCTGCAA
    CATGTGTTCG TGAGGACGGG AGTGGAGTCC ACCCTGGAAA GGAGCCAGAT CACCCGGGAT
    CACATGGGCC AGTTACTGTA TCAGCTGGTG CAGTCAGAAA AGCTCAGCAA ACAGGACTTT
    TTCAGAGGTT TTTCAGAAAC ATTGGAATTG GCAGATGACA TGGCCATTGA TATTCCCCAT
    ATTTGGTTGT ACCTTGCTGA ACTGGTGACT CCCATGTTAA AAGAAGGCGG AATCTCCATG
    AGACAACTCA TCACAGAATT TTGCAAACCT TTACTTCCTG TTGGAAGAGC TGGAATCTTG
    ATTTCTGAAA TATTGCACCT TCTATGCAAA CAAATGAGCC ATAAGAAAGT GGGAGCCCTA
    TGGAGGGAGG CTGACCTCAG CTGGAAGGAC TTTTTACCAG AAGGGGAAGA TGTACATAAT
    TTTCTCTTGG AGCAGAAGTT GGACTTTTTA GAGTCTGACA GTTCCTGTTC CTCCGAAGCA
    CTTTTGAAGA AAGAACTCTC TGCCGAAGAG CTGTATAAGC GACTCGAGAA ACTCATTATT
    GAGGACAAGG CAAATGATGA ACAGATCTTT GACTGGGTAG AGGCTAATCT AGATGAAAGC
    CAGATGAGTT CACCTACATT CCTTAGAGCT TTAATGACAG CCGTCTGTAA AGCAGCTATT
    ATAGCCGACT GTTCGACCTT CCGAGTGGAC ACTGCTGTTA TCAAGCAGAG AGTGCCGATC
    TTACTCAAGT ACCTAGACTC AGATACAGAG AAGGAACTGC AAGCACTTTA TGCACTACAA
    GCATCAATAG TAAAACTTGA TCAACCTGCC AATTTGCTAC GGATGTTTTT TGATTGCCTG
    TATGACGAGG AGGTGATCTC TGAGGATGCC TTCTACAAGT GGGAGAGCAG CAAGGACCCG
    GCAGAGCAGA ATGGGAAGGG TGTGGCCCTG AAGTCTGTCA CGGCGTTCTT CACATGGCTG
    CGGGAAGCAG AAGAGGAGTC TGAGGATAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316370.1
    CDS196..5088
    Translation

    Target ORF information:

    RefSeq Version XM_015460884.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460884.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG GGAGGATTCA GACCCATCCA GTTTTTCCAG 
    AGGCCTCAAA TACAGCCTCC CAGAGCCACC ATCCCGAACA GCAGCCCTTC CATTCGACCC
    GGTGCACAGA CACCCACTGC AGTGTATCAG GCCAATCAGC ACATCATGAT GGTTAACCAT
    CTGCCCATGC CGTACCCAGT GCCCCAGGGT CCTCAGTACT GTATACCACA GTACCGTCAC
    AGTGGCCCTC CGTATGTTGG GCCACCACAA CAGTACCCGG TTCAACCACC AGGACCAGGT
    CCTTTTTATC CTGGGCCAGG ACCTGGGGAC TTCCCCAGTG CTTACGGAAC GCCTTTTTAC
    CCAAGTCAGC CAGTGTACCA GTCAGCACCT ATCATAGTGC CTACGCAGCA GCAGCCCCCT
    CCAGCCAAGA GAGAGAAAAA AACAATAAGA ATTCGGGATC CAAACCAGGG AGGTAAAGAC
    ATAACAGAGG AGATTATGTC TGGAGGTGGC AGCCGAAATC CTACTCCACC CATAGGGAGA
    CCCACTTCCA CACCTACTCC TCCTCAGCAG CTGCCCAGCC AGGTCCCCGA GCACAGCCCT
    GTGGTCTATG GGACTGTGGA GAGCGCTCAT CTTGCTGCCA GCACCCCTGT CACTGCAGCG
    AGCGACCAGA AGCAAGAAGA GAAGCCAAAA CCAGATCCAG TGTTAAAGAC TCCTTCCCCA
    GTCCTTAGGC TAGCCCTCAG TGGAGAGAAG AAAGAACAAG CAGGCCCGAC GTCTGAGATT
    ACTGCAGTAG AGTCCATACC AGAGCTTCCT CTGCCTCCAT CCCCTACTGC TATTTCTCCA
    CTTGCTCGAA GTACAATTAC TTCTCCCACC TCTGTTGCTC TTAGTAGCCA ACCAATATTC
    ACCACTGCAA TTGATGACAG ATGTGAACTC TCTTCCTCAA AAGAAGATAC AATTCCTATT
    CCCAGCCCCA CATCTTGCAC AGAAGCATCA GATCCTTTAC CAACAGATGA AATTGATGAT
    GATATATGCA AGAAATCTTG TAGTGTAGCA CCAAATGATA TTCCACTGAT TTCTAGTACT
    AACCTAATTA ATGAAATGAA TGGAGTTAAT GAAAAATTAC CAGCCACGGA AAGCATTGTG
    GAAATAGTAA AACAGGAGGT GTTACCGCTG ACTCTTGAAT TGGAGATTTT GGAAAATCCC
    CCTGAAGAAA TCAAAGTGGA GTGTGTCCCA ACTCCCATCA CCCCTTCGAT AGTTCCCTCC
    TCTTCTCCGT CTCCTCCAGC TCCTCCAGCT TCTCCTTCTC CCACACCAGT TATCGTTCCT
    GCTGCTGCCA CCAGTGTTAA TACTGGTCGT GGTTCCACCG GAGTCCAGAG AGCCTTCGAA
    GAGGGCGAGA ACATAAGAAC TTGCCTTAGT GAAGATGCAA AAGAGATTCA GAACAAAGTA
    GAGGTAGAAG CAGATGGGCA AACAGAAGAG ATTGTGGATT CTCAGAACTT AAGTTCAAGA
    AAGAGCCCAG TCCCAGCTCA GACAGCTATA CCTGCACCAA AGATGTGGAA GAAACCAAAA
    GACCGGACCC GAGCCACTGA AGAGTCGTTA GAGGCAGAAT CAGAGCCTAA AGCTGAAGAG
    GAGCTTTCAG GTGACAAAAT ACTTGAATCT GAACAGGATA AAATGAGCCA AGGGTTTCAT
    CCTGAAAGAG ACCCCTCTGA CCTAAAAAAC GTGAAAACTG TGGAAGAAAA TGGAGAAGAA
    GCTGAGCCTG TACGTAATGG TGCTGAGAGT ATTTCTGAGG GTGAAGGAAT AGATGCTAAT
    TCAGGCTCCA CTGATAGTTC TGGTGAAGGG GTCACATTCC CATTTAAGTC AGAATCCTGG
    AAGCCTCCTG ATTCGGAAGG CAAGAAGCAG TATGACAGGG AGTTTTTGCT GGATTTCCAA
    TTCATGCCAG CGTGTATTCA GAAACCAGAG GGCCTGCCTC CCATCAGTGA TGTGGTTCTT
    GACAAGATCA ATCAACCAAA ATTGCCAATG CGAACTCTGG ATCCTCGGAT TTTGCCTCGA
    GGACCAGACT TTACTCCAGC CTTTGCAGAT TTTGGAAGGC AAGCCCCTGG TGGAAGAGGT
    GTACCTGTTT GCAAAGTGCA GAGCAGGCAT GGATTGCCAA TTCTGGAACA GAGCAAAGCC
    CCAGCTTCCA CTCCACTGGC GATGTCGCAC CCACCCCTGA AGAGCCTGCC TCTAGGGCTG
    TTGAATGTTG GAGCACGGAG ATCTCAGCCT GGCCAGAGGA GAGAGCCCAG GAAGATCATC
    ACGGTCTCCG TGAAAGAAGA CGTGCACCTG AAGAAGGCAG AGAACGCCTG GAAGCCCAGC
    CAGAGGCGGG ATAGCCCAGC CGAGGACCCC GAGAACATCA AGACCCAGGA ACTTTTTAGA
    AAAGTTCGAA GTATCTTAAA TAAATTGACA CCACAGATGT TCAACCAACT GATGAAGCAA
    GTGTCAGGAC TTACTGTTGA CACAGAGGAG CGGCTGAAAG GAGTCATTGA CCTGGTCTTT
    GAGAAGGCTA TTGACGAACC CAGTTTCTCT GTGGCTTACG CAAACATGTG TCGATGTCTA
    GTAACGCTGA AAGTACCCAT GGCAGACAAG CCTGGTAACA CAGTGAACTT CCGGAAGCTA
    CTACTGAACC GTTGCCAGAA GGAATTTGAA AAAGATAAAG CCGATGATGA TGTCTTTGAG
    AAGAAGCAGA AAGAACTCGA GGCTGCCAGT GCTCCAGAGG AGAGGACAAG GCTTCATGAT
    GAACTGGAAG AAGCTAAAGA TAAAGCCCGG CGGAGATCCA TTGGCAACAT CAAGTTCATT
    GGAGAACTCT TTAAACTCAA AATGCTGACT GAAGCCATCA TGCATGACTG TGTGGTGAAG
    CTGCTGAAGA ACCACGATGA AGAATCCCTC GAGTGTCTGT GTCGCCTGCT CACCACAATT
    GGCAAAGACC TGGACTTTGA GAAAGCAAAG CCACGTATGG ACCAGTATTT TAATCAGATG
    GAGAAAATTG TAAAAGAAAG AAAAACCTCA TCTAGGATTC GATTCATGCT TCAGGACGTA
    ATAGATCTAA GGCTGTGTAA TTGGGTATCA CGACGAGCAG ATCAGGGGCC TAAAACTATT
    GAACAGATTC ACAAAGAAGC TAAAATAGAA GAACAGGAAG AGCAGAGGAA GGTCCAACAA
    CTCATGACCA AAGAGAAGAG AAGACCAGGT GTCCAGAGAG TGGATGAAGG TGGGTGGAAC
    ACTGTTCAAG GGGCCAAGAA TAGTCGGGTA TTGGACCCCT CAAAATTTCT GAAAATCACT
    AAGCCCACAA TTGATGAGAA AATTCAGCTG GTACCTAAAG CGCAGCTGGG CAGCTGGGGA
    AAGGGCAGCA GTGGTGGAGC CAAGGCGAGC GAGGCCGATG CCTTAAGATC AAGTGCTTCC
    AGTTTAAACA GATTCTCTGC TTTGCAACCT CCAGCACCTT CAGGGTCTGC GTCATCCACA
    CCTCTAGAGT TTGATTCCCG GCGGACCTTA ACTAGTCGAG GAAGCACGGG CAGGGAGAAG
    AATGACAAGC CCCTCCAGCC TGCAGCAGCT CGGCCGAACA CTTTCATGAG GGGTGGCAGC
    AGTAAAGACC TGTTAGACAA TCAGTCTCAG GAGGAGCAGC GGAGGGAGAT GCTGGAGACC
    GTGAAGCAGC TCACAGGAGG CATGGACGTG GAGAGGAGCA GCGCTGAGGC GGAGCGAAGC
    AGGACCAGGG AGCCAGTGAC AAAACCGGAA ATTCCAGTAA CATCAGCCTC TGACAAGCCC
    GCGTTGTCAG AGGAAGAGAT GGAGAGGAAG TCCAAATCTA TCATTGATGA GTTTCTACAC
    ATTAATGATT TTAAGGAAGC CATGCAGTGT GTGGAAGAGC TGAACGCCCA GGGCCTGCAA
    CATGTGTTCG TGAGGACGGG AGTGGAGTCC ACCCTGGAAA GGAGCCAGAT CACCCGGGAT
    CACATGGGCC AGTTACTGTA TCAGCTGGTG CAGTCAGAAA AGCTCAGCAA ACAGGACTTT
    TTCAGAGGTT TTTCAGAAAC ATTGGAATTG GCAGATGACA TGGCCATTGA TATTCCCCAT
    ATTTGGTTGT ACCTTGCTGA ACTGGTGACT CCCATGTTAA AAGAAGGCGG AATCTCCATG
    AGACAACTCA TCACAGAATT TTGCAAACCT TTACTTCCTG TTGGAAGAGC TGGAATCTTG
    ATTTCTGAAA TATTGCACCT TCTATGCAAA CAAATGAGCC ATAAGAAAGT GGGAGCCCTA
    TGGAGGGAGG CTGACCTCAG CTGGAAGGAC TTTTTACCAG AAGGGGAAGA TGTACATAAT
    TTTCTCTTGG AGCAGAAGTT GGACTTTTTA GAGTCTGACA GTTCCTGTTC CTCCGAAGCA
    CTTTTGAAGA AAGAACTCTC TGCCGAAGAG CTGTATAAGC GACTCGAGAA ACTCATTATT
    GAGGACAAGG CAAATGATGA ACAGATCTTT GACTGGGTAG AGGCTAATCT AGATGAAAGC
    CAGATGAGTT CACCTACATT CCTTAGAGCT TTAATGACAG CCGTCTGTAA AGCAGCTATT
    ATAGCCGACT GTTCGACCTT CCGAGTGGAC ACTGCTGTTA TCAAGCAGAG AGTGCCGATC
    TTACTCAAGT ACCTAGACTC AGATACAGAG AAGGAACTGC AAGCACTTTA TGCACTACAA
    GCATCAATAG TAAAACTTGA TCAACCTGCC AATTTGCTAC GGATGTTTTT TGATTGCCTG
    TATGACGAGG AGGTGATCTC TGAGGATGCC TTCTACAAGT GGGAGAGCAG CAAGGACCCG
    GCAGAGCAGA ATGGGAAGGG TGTGGCCCTG AAGTCTGTCA CGGCGTTCTT CACATGGCTG
    CGGGAAGCAG AAGAGGAGTC TGAGGATAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88096
    Clone ID Related Accession (Same CDS sequence) XM_015460886.2 , XM_024983008.1 , XM_015460886.1 , XM_015462482.1 , XM_024983012.1
    Accession Version XM_015460886.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTTTCCAGA GGCCTCAAAT ACAGCCTCCC 
    AGAGCCACCA TCCCGAACAG CAGCCCTTCC ATTCGACCCG GTGCACAGAC ACCCACTGCA
    GTGTATCAGG CCAATCAGCA CATCATGATG GTTAACCATC TGCCCATGCC GTACCCAGTG
    CCCCAGGGTC CTCAGTACTG TATACCACAG TACCGTCACA GTGGCCCTCC GTATGTTGGG
    CCACCACAAC AGTACCCGGT TCAACCACCA GGACCAGGTC CTTTTTATCC TGGGCCAGGA
    CCTGGGGACT TCCCCAGTGC TTACGGAACG CCTTTTTACC CAAGTCAGCC AGTGTACCAG
    TCAGCACCTA TCATAGTGCC TACGCAGCAG CAGCCCCCTC CAGCCAAGAG AGAGAAAAAA
    ACAATAAGAA TTCGGGATCC AAACCAGGGA GGTAAAGACA TAACAGAGGA GATTATGTCT
    GGAGGTGGCA GCCGAAATCC TACTCCACCC ATAGGGAGAC CCACTTCCAC ACCTACTCCT
    CCTCAGCAGC TGCCCAGCCA GGTCCCCGAG CACAGCCCTG TGGTCTATGG GACTGTGGAG
    AGCGCTCATC TTGCTGCCAG CACCCCTGTC ACTGCAGCGA GCGACCAGAA GCAAGAAGAG
    AAGCCAAAAC CAGATCCAGT GTTAAAGACT CCTTCCCCAG TCCTTAGGCT AGCCCTCAGT
    GGAGAGAAGA AAGAACAAGC AGGCCCGACG TCTGAGATTA CTGCAGTAGA GTCCATACCA
    GAGCTTCCTC TGCCTCCATC CCCTACTGCT ATTTCTCCAC TTGCTCGAAG TACAATTACT
    TCTCCCACCT CTGTTGCTCT TAGTAGCCAA CCAATATTCA CCACTGCAAT TGATGACAGA
    TGTGAACTCT CTTCCTCAAA AGAAGATACA ATTCCTATTC CCAGCCCCAC ATCTTGCACA
    GAAGCATCAG ATCCTTTACC AACAGATGAA ATTGATGATG ATATATGCAA GAAATCTTGT
    AGTGTAGCAC CAAATGATAT TCCACTGATT TCTAGTACTA ACCTAATTAA TGAAATGAAT
    GGAGTTAATG AAAAATTACC AGCCACGGAA AGCATTGTGG AAATAGTAAA ACAGGAGGTG
    TTACCGCTGA CTCTTGAATT GGAGATTTTG GAAAATCCCC CTGAAGAAAT CAAAGTGGAG
    TGTGTCCCAA CTCCCATCAC CCCTTCGATA GTTCCCTCCT CTTCTCCGTC TCCTCCAGCT
    CCTCCAGCTT CTCCTTCTCC CACACCAGTT ATCGTTCCTG CTGCTGCCAC CAGTGTTAAT
    ACTGGTCGTG GTTCCACCGG AGTCCAGAGA GCCTTCGAAG AGGGCGAGAA CATAAGAACT
    TGCCTTAGTG AAGATGCAAA AGAGATTCAG AACAAAGTAG AGGTAGAAGC AGATGGGCAA
    ACAGAAGAGA TTGTGGATTC TCAGAACTTA AGTTCAAGAA AGAGCCCAGT CCCAGCTCAG
    ACAGCTATAC CTGCACCAAA GATGTGGAAG AAACCAAAAG ACCGGACCCG AGCCACTGAA
    GAGTCGTTAG AGGCAGAATC AGAGCCTAAA GCTGAAGAGG AGCTTTCAGG TGACAAAATA
    CTTGAATCTG AACAGGATAA AATGAGCCAA GGGTTTCATC CTGAAAGAGA CCCCTCTGAC
    CTAAAAAACG TGAAAACTGT GGAAGAAAAT GGAGAAGAAG CTGAGCCTGT ACGTAATGGT
    GCTGAGAGTA TTTCTGAGGG TGAAGGAATA GATGCTAATT CAGGCTCCAC TGATAGTTCT
    GGTGAAGGGG TCACATTCCC ATTTAAGTCA GAATCCTGGA AGCCTCCTGA TTCGGAAGGC
    AAGAAGCAGT ATGACAGGGA GTTTTTGCTG GATTTCCAAT TCATGCCAGC GTGTATTCAG
    AAACCAGAGG GCCTGCCTCC CATCAGTGAT GTGGTTCTTG ACAAGATCAA TCAACCAAAA
    TTGCCAATGC GAACTCTGGA TCCTCGGATT TTGCCTCGAG GACCAGACTT TACTCCAGCC
    TTTGCAGATT TTGGAAGGCA AGCCCCTGGT GGAAGAGGTG TACCTGTTTG CAAAGTGCAG
    AGCAGGCATG GATTGCCAAT TCTGGAACAG AGCAAAGCCC CAGCTTCCAC TCCACTGGCG
    ATGTCGCACC CACCCCTGAA GAGCCTGCCT CTAGGGCTGT TGAATGTTGG AGCACGGAGA
    TCTCAGCCTG GCCAGAGGAG AGAGCCCAGG AAGATCATCA CGGTCTCCGT GAAAGAAGAC
    GTGCACCTGA AGAAGGCAGA GAACGCCTGG AAGCCCAGCC AGAGGCGGGA TAGCCCAGCC
    GAGGACCCCG AGAACATCAA GACCCAGGAA CTTTTTAGAA AAGTTCGAAG TATCTTAAAT
    AAATTGACAC CACAGATGTT CAACCAACTG ATGAAGCAAG TGTCAGGACT TACTGTTGAC
    ACAGAGGAGC GGCTGAAAGG AGTCATTGAC CTGGTCTTTG AGAAGGCTAT TGACGAACCC
    AGTTTCTCTG TGGCTTACGC AAACATGTGT CGATGTCTAG TAACGCTGAA AGTACCCATG
    GCAGACAAGC CTGGTAACAC AGTGAACTTC CGGAAGCTAC TACTGAACCG TTGCCAGAAG
    GAATTTGAAA AAGATAAAGC CGATGATGAT GTCTTTGAGA AGAAGCAGAA AGAACTCGAG
    GCTGCCAGTG CTCCAGAGGA GAGGACAAGG CTTCATGATG AACTGGAAGA AGCTAAAGAT
    AAAGCCCGGC GGAGATCCAT TGGCAACATC AAGTTCATTG GAGAACTCTT TAAACTCAAA
    ATGCTGACTG AAGCCATCAT GCATGACTGT GTGGTGAAGC TGCTGAAGAA CCACGATGAA
    GAATCCCTCG AGTGTCTGTG TCGCCTGCTC ACCACAATTG GCAAAGACCT GGACTTTGAG
    AAAGCAAAGC CACGTATGGA CCAGTATTTT AATCAGATGG AGAAAATTGT AAAAGAAAGA
    AAAACCTCAT CTAGGATTCG ATTCATGCTT CAGGACGTAA TAGATCTAAG GCTGTGTAAT
    TGGGTATCAC GACGAGCAGA TCAGGGGCCT AAAACTATTG AACAGATTCA CAAAGAAGCT
    AAAATAGAAG AACAGGAAGA GCAGAGGAAG GTCCAACAAC TCATGACCAA AGAGAAGAGA
    AGACCAGGTG TCCAGAGAGT GGATGAAGGT GGGTGGAACA CTGTTCAAGG GGCCAAGAAT
    AGTCGGGTAT TGGACCCCTC AAAATTTCTG AAAATCACTA AGCCCACAAT TGATGAGAAA
    ATTCAGCTGG TACCTAAAGC GCAGCTGGGC AGCTGGGGAA AGGGCAGCAG TGGTGGAGCC
    AAGGCGAGCG AGGCCGATGC CTTAAGATCA AGTGCTTCCA GTTTAAACAG ATTCTCTGCT
    TTGCAACCTC CAGCACCTTC AGGGTCTGCG TCATCCACAC CTCTAGAGTT TGATTCCCGG
    CGGACCTTAA CTAGTCGAGG AAGCACGGGC AGGGAGAAGA ATGACAAGCC CCTCCAGCCT
    GCAGCAGCTC GGCCGAACAC TTTCATGAGG GGTGGCAGCA GTAAAGACCT GTTAGACAAT
    CAGTCTCAGG AGGAGCAGCG GAGGGAGATG CTGGAGACCG TGAAGCAGCT CACAGGAGGC
    ATGGACGTGG AGAGGAGCAG CGCTGAGGCG GAGCGAAGCA GGACCAGGGA GCCAGTGACA
    AAACCGGAAA TTCCAGTAAC ATCAGCCTCT GACAAGCCCG CGTTGTCAGA GGAAGAGATG
    GAGAGGAAGT CCAAATCTAT CATTGATGAG TTTCTACACA TTAATGATTT TAAGGAAGCC
    ATGCAGTGTG TGGAAGAGCT GAACGCCCAG GGCCTGCAAC ATGTGTTCGT GAGGACGGGA
    GTGGAGTCCA CCCTGGAAAG GAGCCAGATC ACCCGGGATC ACATGGGCCA GTTACTGTAT
    CAGCTGGTGC AGTCAGAAAA GCTCAGCAAA CAGGACTTTT TCAGAGGTTT TTCAGAAACA
    TTGGAATTGG CAGATGACAT GGCCATTGAT ATTCCCCATA TTTGGTTGTA CCTTGCTGAA
    CTGGTGACTC CCATGTTAAA AGAAGGCGGA ATCTCCATGA GACAACTCAT CACAGAATTT
    TGCAAACCTT TACTTCCTGT TGGAAGAGCT GGAATCTTGA TTTCTGAAAT ATTGCACCTT
    CTATGCAAAC AAATGAGCCA TAAGAAAGTG GGAGCCCTAT GGAGGGAGGC TGACCTCAGC
    TGGAAGGACT TTTTACCAGA AGGGGAAGAT GTACATAATT TTCTCTTGGA GCAGAAGTTG
    GACTTTTTAG AGTCTGACAG TTCCTGTTCC TCCGAAGCAC TTTTGAAGAA AGAACTCTCT
    GCCGAAGAGC TGTATAAGCG ACTCGAGAAA CTCATTATTG AGGACAAGGC AAATGATGAA
    CAGATCTTTG ACTGGGTAGA GGCTAATCTA GATGAAAGCC AGATGAGTTC ACCTACATTC
    CTTAGAGCTT TAATGACAGC CGTCTGTAAA GCAGCTATTA TAGCCGACTG TTCGACCTTC
    CGAGTGGACA CTGCTGTTAT CAAGCAGAGA GTGCCGATCT TACTCAAGTA CCTAGACTCA
    GATACAGAGA AGGAACTGCA AGCACTTTAT GCACTACAAG CATCAATAGT AAAACTTGAT
    CAACCTGCCA ATTTGCTACG GATGTTTTTT GATTGCCTGT ATGACGAGGA GGTGATCTCT
    GAGGATGCCT TCTACAAGTG GGAGAGCAGC AAGGACCCGG CAGAGCAGAA TGGGAAGGGT
    GTGGCCCTGA AGTCTGTCAC GGCGTTCTTC ACATGGCTGC GGGAAGCAGA AGAGGAGTCT
    GAGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316372.1
    CDS232..5103
    Translation

    Target ORF information:

    RefSeq Version XM_015460886.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460886.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTTTCCAGA GGCCTCAAAT ACAGCCTCCC 
    AGAGCCACCA TCCCGAACAG CAGCCCTTCC ATTCGACCCG GTGCACAGAC ACCCACTGCA
    GTGTATCAGG CCAATCAGCA CATCATGATG GTTAACCATC TGCCCATGCC GTACCCAGTG
    CCCCAGGGTC CTCAGTACTG TATACCACAG TACCGTCACA GTGGCCCTCC GTATGTTGGG
    CCACCACAAC AGTACCCGGT TCAACCACCA GGACCAGGTC CTTTTTATCC TGGGCCAGGA
    CCTGGGGACT TCCCCAGTGC TTACGGAACG CCTTTTTACC CAAGTCAGCC AGTGTACCAG
    TCAGCACCTA TCATAGTGCC TACGCAGCAG CAGCCCCCTC CAGCCAAGAG AGAGAAAAAA
    ACAATAAGAA TTCGGGATCC AAACCAGGGA GGTAAAGACA TAACAGAGGA GATTATGTCT
    GGAGGTGGCA GCCGAAATCC TACTCCACCC ATAGGGAGAC CCACTTCCAC ACCTACTCCT
    CCTCAGCAGC TGCCCAGCCA GGTCCCCGAG CACAGCCCTG TGGTCTATGG GACTGTGGAG
    AGCGCTCATC TTGCTGCCAG CACCCCTGTC ACTGCAGCGA GCGACCAGAA GCAAGAAGAG
    AAGCCAAAAC CAGATCCAGT GTTAAAGACT CCTTCCCCAG TCCTTAGGCT AGCCCTCAGT
    GGAGAGAAGA AAGAACAAGC AGGCCCGACG TCTGAGATTA CTGCAGTAGA GTCCATACCA
    GAGCTTCCTC TGCCTCCATC CCCTACTGCT ATTTCTCCAC TTGCTCGAAG TACAATTACT
    TCTCCCACCT CTGTTGCTCT TAGTAGCCAA CCAATATTCA CCACTGCAAT TGATGACAGA
    TGTGAACTCT CTTCCTCAAA AGAAGATACA ATTCCTATTC CCAGCCCCAC ATCTTGCACA
    GAAGCATCAG ATCCTTTACC AACAGATGAA ATTGATGATG ATATATGCAA GAAATCTTGT
    AGTGTAGCAC CAAATGATAT TCCACTGATT TCTAGTACTA ACCTAATTAA TGAAATGAAT
    GGAGTTAATG AAAAATTACC AGCCACGGAA AGCATTGTGG AAATAGTAAA ACAGGAGGTG
    TTACCGCTGA CTCTTGAATT GGAGATTTTG GAAAATCCCC CTGAAGAAAT CAAAGTGGAG
    TGTGTCCCAA CTCCCATCAC CCCTTCGATA GTTCCCTCCT CTTCTCCGTC TCCTCCAGCT
    CCTCCAGCTT CTCCTTCTCC CACACCAGTT ATCGTTCCTG CTGCTGCCAC CAGTGTTAAT
    ACTGGTCGTG GTTCCACCGG AGTCCAGAGA GCCTTCGAAG AGGGCGAGAA CATAAGAACT
    TGCCTTAGTG AAGATGCAAA AGAGATTCAG AACAAAGTAG AGGTAGAAGC AGATGGGCAA
    ACAGAAGAGA TTGTGGATTC TCAGAACTTA AGTTCAAGAA AGAGCCCAGT CCCAGCTCAG
    ACAGCTATAC CTGCACCAAA GATGTGGAAG AAACCAAAAG ACCGGACCCG AGCCACTGAA
    GAGTCGTTAG AGGCAGAATC AGAGCCTAAA GCTGAAGAGG AGCTTTCAGG TGACAAAATA
    CTTGAATCTG AACAGGATAA AATGAGCCAA GGGTTTCATC CTGAAAGAGA CCCCTCTGAC
    CTAAAAAACG TGAAAACTGT GGAAGAAAAT GGAGAAGAAG CTGAGCCTGT ACGTAATGGT
    GCTGAGAGTA TTTCTGAGGG TGAAGGAATA GATGCTAATT CAGGCTCCAC TGATAGTTCT
    GGTGAAGGGG TCACATTCCC ATTTAAGTCA GAATCCTGGA AGCCTCCTGA TTCGGAAGGC
    AAGAAGCAGT ATGACAGGGA GTTTTTGCTG GATTTCCAAT TCATGCCAGC GTGTATTCAG
    AAACCAGAGG GCCTGCCTCC CATCAGTGAT GTGGTTCTTG ACAAGATCAA TCAACCAAAA
    TTGCCAATGC GAACTCTGGA TCCTCGGATT TTGCCTCGAG GACCAGACTT TACTCCAGCC
    TTTGCAGATT TTGGAAGGCA AGCCCCTGGT GGAAGAGGTG TACCTGTTTG CAAAGTGCAG
    AGCAGGCATG GATTGCCAAT TCTGGAACAG AGCAAAGCCC CAGCTTCCAC TCCACTGGCG
    ATGTCGCACC CACCCCTGAA GAGCCTGCCT CTAGGGCTGT TGAATGTTGG AGCACGGAGA
    TCTCAGCCTG GCCAGAGGAG AGAGCCCAGG AAGATCATCA CGGTCTCCGT GAAAGAAGAC
    GTGCACCTGA AGAAGGCAGA GAACGCCTGG AAGCCCAGCC AGAGGCGGGA TAGCCCAGCC
    GAGGACCCCG AGAACATCAA GACCCAGGAA CTTTTTAGAA AAGTTCGAAG TATCTTAAAT
    AAATTGACAC CACAGATGTT CAACCAACTG ATGAAGCAAG TGTCAGGACT TACTGTTGAC
    ACAGAGGAGC GGCTGAAAGG AGTCATTGAC CTGGTCTTTG AGAAGGCTAT TGACGAACCC
    AGTTTCTCTG TGGCTTACGC AAACATGTGT CGATGTCTAG TAACGCTGAA AGTACCCATG
    GCAGACAAGC CTGGTAACAC AGTGAACTTC CGGAAGCTAC TACTGAACCG TTGCCAGAAG
    GAATTTGAAA AAGATAAAGC CGATGATGAT GTCTTTGAGA AGAAGCAGAA AGAACTCGAG
    GCTGCCAGTG CTCCAGAGGA GAGGACAAGG CTTCATGATG AACTGGAAGA AGCTAAAGAT
    AAAGCCCGGC GGAGATCCAT TGGCAACATC AAGTTCATTG GAGAACTCTT TAAACTCAAA
    ATGCTGACTG AAGCCATCAT GCATGACTGT GTGGTGAAGC TGCTGAAGAA CCACGATGAA
    GAATCCCTCG AGTGTCTGTG TCGCCTGCTC ACCACAATTG GCAAAGACCT GGACTTTGAG
    AAAGCAAAGC CACGTATGGA CCAGTATTTT AATCAGATGG AGAAAATTGT AAAAGAAAGA
    AAAACCTCAT CTAGGATTCG ATTCATGCTT CAGGACGTAA TAGATCTAAG GCTGTGTAAT
    TGGGTATCAC GACGAGCAGA TCAGGGGCCT AAAACTATTG AACAGATTCA CAAAGAAGCT
    AAAATAGAAG AACAGGAAGA GCAGAGGAAG GTCCAACAAC TCATGACCAA AGAGAAGAGA
    AGACCAGGTG TCCAGAGAGT GGATGAAGGT GGGTGGAACA CTGTTCAAGG GGCCAAGAAT
    AGTCGGGTAT TGGACCCCTC AAAATTTCTG AAAATCACTA AGCCCACAAT TGATGAGAAA
    ATTCAGCTGG TACCTAAAGC GCAGCTGGGC AGCTGGGGAA AGGGCAGCAG TGGTGGAGCC
    AAGGCGAGCG AGGCCGATGC CTTAAGATCA AGTGCTTCCA GTTTAAACAG ATTCTCTGCT
    TTGCAACCTC CAGCACCTTC AGGGTCTGCG TCATCCACAC CTCTAGAGTT TGATTCCCGG
    CGGACCTTAA CTAGTCGAGG AAGCACGGGC AGGGAGAAGA ATGACAAGCC CCTCCAGCCT
    GCAGCAGCTC GGCCGAACAC TTTCATGAGG GGTGGCAGCA GTAAAGACCT GTTAGACAAT
    CAGTCTCAGG AGGAGCAGCG GAGGGAGATG CTGGAGACCG TGAAGCAGCT CACAGGAGGC
    ATGGACGTGG AGAGGAGCAG CGCTGAGGCG GAGCGAAGCA GGACCAGGGA GCCAGTGACA
    AAACCGGAAA TTCCAGTAAC ATCAGCCTCT GACAAGCCCG CGTTGTCAGA GGAAGAGATG
    GAGAGGAAGT CCAAATCTAT CATTGATGAG TTTCTACACA TTAATGATTT TAAGGAAGCC
    ATGCAGTGTG TGGAAGAGCT GAACGCCCAG GGCCTGCAAC ATGTGTTCGT GAGGACGGGA
    GTGGAGTCCA CCCTGGAAAG GAGCCAGATC ACCCGGGATC ACATGGGCCA GTTACTGTAT
    CAGCTGGTGC AGTCAGAAAA GCTCAGCAAA CAGGACTTTT TCAGAGGTTT TTCAGAAACA
    TTGGAATTGG CAGATGACAT GGCCATTGAT ATTCCCCATA TTTGGTTGTA CCTTGCTGAA
    CTGGTGACTC CCATGTTAAA AGAAGGCGGA ATCTCCATGA GACAACTCAT CACAGAATTT
    TGCAAACCTT TACTTCCTGT TGGAAGAGCT GGAATCTTGA TTTCTGAAAT ATTGCACCTT
    CTATGCAAAC AAATGAGCCA TAAGAAAGTG GGAGCCCTAT GGAGGGAGGC TGACCTCAGC
    TGGAAGGACT TTTTACCAGA AGGGGAAGAT GTACATAATT TTCTCTTGGA GCAGAAGTTG
    GACTTTTTAG AGTCTGACAG TTCCTGTTCC TCCGAAGCAC TTTTGAAGAA AGAACTCTCT
    GCCGAAGAGC TGTATAAGCG ACTCGAGAAA CTCATTATTG AGGACAAGGC AAATGATGAA
    CAGATCTTTG ACTGGGTAGA GGCTAATCTA GATGAAAGCC AGATGAGTTC ACCTACATTC
    CTTAGAGCTT TAATGACAGC CGTCTGTAAA GCAGCTATTA TAGCCGACTG TTCGACCTTC
    CGAGTGGACA CTGCTGTTAT CAAGCAGAGA GTGCCGATCT TACTCAAGTA CCTAGACTCA
    GATACAGAGA AGGAACTGCA AGCACTTTAT GCACTACAAG CATCAATAGT AAAACTTGAT
    CAACCTGCCA ATTTGCTACG GATGTTTTTT GATTGCCTGT ATGACGAGGA GGTGATCTCT
    GAGGATGCCT TCTACAAGTG GGAGAGCAGC AAGGACCCGG CAGAGCAGAA TGGGAAGGGT
    GTGGCCCTGA AGTCTGTCAC GGCGTTCTTC ACATGGCTGC GGGAAGCAGA AGAGGAGTCT
    GAGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88096
    Clone ID Related Accession (Same CDS sequence) XM_015460886.2 , XM_024983008.1 , XM_015460886.1 , XM_015462482.1 , XM_024983012.1
    Accession Version XM_024983008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTTTCCAGA GGCCTCAAAT ACAGCCTCCC 
    AGAGCCACCA TCCCGAACAG CAGCCCTTCC ATTCGACCCG GTGCACAGAC ACCCACTGCA
    GTGTATCAGG CCAATCAGCA CATCATGATG GTTAACCATC TGCCCATGCC GTACCCAGTG
    CCCCAGGGTC CTCAGTACTG TATACCACAG TACCGTCACA GTGGCCCTCC GTATGTTGGG
    CCACCACAAC AGTACCCGGT TCAACCACCA GGACCAGGTC CTTTTTATCC TGGGCCAGGA
    CCTGGGGACT TCCCCAGTGC TTACGGAACG CCTTTTTACC CAAGTCAGCC AGTGTACCAG
    TCAGCACCTA TCATAGTGCC TACGCAGCAG CAGCCCCCTC CAGCCAAGAG AGAGAAAAAA
    ACAATAAGAA TTCGGGATCC AAACCAGGGA GGTAAAGACA TAACAGAGGA GATTATGTCT
    GGAGGTGGCA GCCGAAATCC TACTCCACCC ATAGGGAGAC CCACTTCCAC ACCTACTCCT
    CCTCAGCAGC TGCCCAGCCA GGTCCCCGAG CACAGCCCTG TGGTCTATGG GACTGTGGAG
    AGCGCTCATC TTGCTGCCAG CACCCCTGTC ACTGCAGCGA GCGACCAGAA GCAAGAAGAG
    AAGCCAAAAC CAGATCCAGT GTTAAAGACT CCTTCCCCAG TCCTTAGGCT AGCCCTCAGT
    GGAGAGAAGA AAGAACAAGC AGGCCCGACG TCTGAGATTA CTGCAGTAGA GTCCATACCA
    GAGCTTCCTC TGCCTCCATC CCCTACTGCT ATTTCTCCAC TTGCTCGAAG TACAATTACT
    TCTCCCACCT CTGTTGCTCT TAGTAGCCAA CCAATATTCA CCACTGCAAT TGATGACAGA
    TGTGAACTCT CTTCCTCAAA AGAAGATACA ATTCCTATTC CCAGCCCCAC ATCTTGCACA
    GAAGCATCAG ATCCTTTACC AACAGATGAA ATTGATGATG ATATATGCAA GAAATCTTGT
    AGTGTAGCAC CAAATGATAT TCCACTGATT TCTAGTACTA ACCTAATTAA TGAAATGAAT
    GGAGTTAATG AAAAATTACC AGCCACGGAA AGCATTGTGG AAATAGTAAA ACAGGAGGTG
    TTACCGCTGA CTCTTGAATT GGAGATTTTG GAAAATCCCC CTGAAGAAAT CAAAGTGGAG
    TGTGTCCCAA CTCCCATCAC CCCTTCGATA GTTCCCTCCT CTTCTCCGTC TCCTCCAGCT
    CCTCCAGCTT CTCCTTCTCC CACACCAGTT ATCGTTCCTG CTGCTGCCAC CAGTGTTAAT
    ACTGGTCGTG GTTCCACCGG AGTCCAGAGA GCCTTCGAAG AGGGCGAGAA CATAAGAACT
    TGCCTTAGTG AAGATGCAAA AGAGATTCAG AACAAAGTAG AGGTAGAAGC AGATGGGCAA
    ACAGAAGAGA TTGTGGATTC TCAGAACTTA AGTTCAAGAA AGAGCCCAGT CCCAGCTCAG
    ACAGCTATAC CTGCACCAAA GATGTGGAAG AAACCAAAAG ACCGGACCCG AGCCACTGAA
    GAGTCGTTAG AGGCAGAATC AGAGCCTAAA GCTGAAGAGG AGCTTTCAGG TGACAAAATA
    CTTGAATCTG AACAGGATAA AATGAGCCAA GGGTTTCATC CTGAAAGAGA CCCCTCTGAC
    CTAAAAAACG TGAAAACTGT GGAAGAAAAT GGAGAAGAAG CTGAGCCTGT ACGTAATGGT
    GCTGAGAGTA TTTCTGAGGG TGAAGGAATA GATGCTAATT CAGGCTCCAC TGATAGTTCT
    GGTGAAGGGG TCACATTCCC ATTTAAGTCA GAATCCTGGA AGCCTCCTGA TTCGGAAGGC
    AAGAAGCAGT ATGACAGGGA GTTTTTGCTG GATTTCCAAT TCATGCCAGC GTGTATTCAG
    AAACCAGAGG GCCTGCCTCC CATCAGTGAT GTGGTTCTTG ACAAGATCAA TCAACCAAAA
    TTGCCAATGC GAACTCTGGA TCCTCGGATT TTGCCTCGAG GACCAGACTT TACTCCAGCC
    TTTGCAGATT TTGGAAGGCA AGCCCCTGGT GGAAGAGGTG TACCTGTTTG CAAAGTGCAG
    AGCAGGCATG GATTGCCAAT TCTGGAACAG AGCAAAGCCC CAGCTTCCAC TCCACTGGCG
    ATGTCGCACC CACCCCTGAA GAGCCTGCCT CTAGGGCTGT TGAATGTTGG AGCACGGAGA
    TCTCAGCCTG GCCAGAGGAG AGAGCCCAGG AAGATCATCA CGGTCTCCGT GAAAGAAGAC
    GTGCACCTGA AGAAGGCAGA GAACGCCTGG AAGCCCAGCC AGAGGCGGGA TAGCCCAGCC
    GAGGACCCCG AGAACATCAA GACCCAGGAA CTTTTTAGAA AAGTTCGAAG TATCTTAAAT
    AAATTGACAC CACAGATGTT CAACCAACTG ATGAAGCAAG TGTCAGGACT TACTGTTGAC
    ACAGAGGAGC GGCTGAAAGG AGTCATTGAC CTGGTCTTTG AGAAGGCTAT TGACGAACCC
    AGTTTCTCTG TGGCTTACGC AAACATGTGT CGATGTCTAG TAACGCTGAA AGTACCCATG
    GCAGACAAGC CTGGTAACAC AGTGAACTTC CGGAAGCTAC TACTGAACCG TTGCCAGAAG
    GAATTTGAAA AAGATAAAGC CGATGATGAT GTCTTTGAGA AGAAGCAGAA AGAACTCGAG
    GCTGCCAGTG CTCCAGAGGA GAGGACAAGG CTTCATGATG AACTGGAAGA AGCTAAAGAT
    AAAGCCCGGC GGAGATCCAT TGGCAACATC AAGTTCATTG GAGAACTCTT TAAACTCAAA
    ATGCTGACTG AAGCCATCAT GCATGACTGT GTGGTGAAGC TGCTGAAGAA CCACGATGAA
    GAATCCCTCG AGTGTCTGTG TCGCCTGCTC ACCACAATTG GCAAAGACCT GGACTTTGAG
    AAAGCAAAGC CACGTATGGA CCAGTATTTT AATCAGATGG AGAAAATTGT AAAAGAAAGA
    AAAACCTCAT CTAGGATTCG ATTCATGCTT CAGGACGTAA TAGATCTAAG GCTGTGTAAT
    TGGGTATCAC GACGAGCAGA TCAGGGGCCT AAAACTATTG AACAGATTCA CAAAGAAGCT
    AAAATAGAAG AACAGGAAGA GCAGAGGAAG GTCCAACAAC TCATGACCAA AGAGAAGAGA
    AGACCAGGTG TCCAGAGAGT GGATGAAGGT GGGTGGAACA CTGTTCAAGG GGCCAAGAAT
    AGTCGGGTAT TGGACCCCTC AAAATTTCTG AAAATCACTA AGCCCACAAT TGATGAGAAA
    ATTCAGCTGG TACCTAAAGC GCAGCTGGGC AGCTGGGGAA AGGGCAGCAG TGGTGGAGCC
    AAGGCGAGCG AGGCCGATGC CTTAAGATCA AGTGCTTCCA GTTTAAACAG ATTCTCTGCT
    TTGCAACCTC CAGCACCTTC AGGGTCTGCG TCATCCACAC CTCTAGAGTT TGATTCCCGG
    CGGACCTTAA CTAGTCGAGG AAGCACGGGC AGGGAGAAGA ATGACAAGCC CCTCCAGCCT
    GCAGCAGCTC GGCCGAACAC TTTCATGAGG GGTGGCAGCA GTAAAGACCT GTTAGACAAT
    CAGTCTCAGG AGGAGCAGCG GAGGGAGATG CTGGAGACCG TGAAGCAGCT CACAGGAGGC
    ATGGACGTGG AGAGGAGCAG CGCTGAGGCG GAGCGAAGCA GGACCAGGGA GCCAGTGACA
    AAACCGGAAA TTCCAGTAAC ATCAGCCTCT GACAAGCCCG CGTTGTCAGA GGAAGAGATG
    GAGAGGAAGT CCAAATCTAT CATTGATGAG TTTCTACACA TTAATGATTT TAAGGAAGCC
    ATGCAGTGTG TGGAAGAGCT GAACGCCCAG GGCCTGCAAC ATGTGTTCGT GAGGACGGGA
    GTGGAGTCCA CCCTGGAAAG GAGCCAGATC ACCCGGGATC ACATGGGCCA GTTACTGTAT
    CAGCTGGTGC AGTCAGAAAA GCTCAGCAAA CAGGACTTTT TCAGAGGTTT TTCAGAAACA
    TTGGAATTGG CAGATGACAT GGCCATTGAT ATTCCCCATA TTTGGTTGTA CCTTGCTGAA
    CTGGTGACTC CCATGTTAAA AGAAGGCGGA ATCTCCATGA GACAACTCAT CACAGAATTT
    TGCAAACCTT TACTTCCTGT TGGAAGAGCT GGAATCTTGA TTTCTGAAAT ATTGCACCTT
    CTATGCAAAC AAATGAGCCA TAAGAAAGTG GGAGCCCTAT GGAGGGAGGC TGACCTCAGC
    TGGAAGGACT TTTTACCAGA AGGGGAAGAT GTACATAATT TTCTCTTGGA GCAGAAGTTG
    GACTTTTTAG AGTCTGACAG TTCCTGTTCC TCCGAAGCAC TTTTGAAGAA AGAACTCTCT
    GCCGAAGAGC TGTATAAGCG ACTCGAGAAA CTCATTATTG AGGACAAGGC AAATGATGAA
    CAGATCTTTG ACTGGGTAGA GGCTAATCTA GATGAAAGCC AGATGAGTTC ACCTACATTC
    CTTAGAGCTT TAATGACAGC CGTCTGTAAA GCAGCTATTA TAGCCGACTG TTCGACCTTC
    CGAGTGGACA CTGCTGTTAT CAAGCAGAGA GTGCCGATCT TACTCAAGTA CCTAGACTCA
    GATACAGAGA AGGAACTGCA AGCACTTTAT GCACTACAAG CATCAATAGT AAAACTTGAT
    CAACCTGCCA ATTTGCTACG GATGTTTTTT GATTGCCTGT ATGACGAGGA GGTGATCTCT
    GAGGATGCCT TCTACAAGTG GGAGAGCAGC AAGGACCCGG CAGAGCAGAA TGGGAAGGGT
    GTGGCCCTGA AGTCTGTCAC GGCGTTCTTC ACATGGCTGC GGGAAGCAGA AGAGGAGTCT
    GAGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838776.1
    CDS76..4947
    Translation

    Target ORF information:

    RefSeq Version XM_024983008.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTTTCCAGA GGCCTCAAAT ACAGCCTCCC 
    AGAGCCACCA TCCCGAACAG CAGCCCTTCC ATTCGACCCG GTGCACAGAC ACCCACTGCA
    GTGTATCAGG CCAATCAGCA CATCATGATG GTTAACCATC TGCCCATGCC GTACCCAGTG
    CCCCAGGGTC CTCAGTACTG TATACCACAG TACCGTCACA GTGGCCCTCC GTATGTTGGG
    CCACCACAAC AGTACCCGGT TCAACCACCA GGACCAGGTC CTTTTTATCC TGGGCCAGGA
    CCTGGGGACT TCCCCAGTGC TTACGGAACG CCTTTTTACC CAAGTCAGCC AGTGTACCAG
    TCAGCACCTA TCATAGTGCC TACGCAGCAG CAGCCCCCTC CAGCCAAGAG AGAGAAAAAA
    ACAATAAGAA TTCGGGATCC AAACCAGGGA GGTAAAGACA TAACAGAGGA GATTATGTCT
    GGAGGTGGCA GCCGAAATCC TACTCCACCC ATAGGGAGAC CCACTTCCAC ACCTACTCCT
    CCTCAGCAGC TGCCCAGCCA GGTCCCCGAG CACAGCCCTG TGGTCTATGG GACTGTGGAG
    AGCGCTCATC TTGCTGCCAG CACCCCTGTC ACTGCAGCGA GCGACCAGAA GCAAGAAGAG
    AAGCCAAAAC CAGATCCAGT GTTAAAGACT CCTTCCCCAG TCCTTAGGCT AGCCCTCAGT
    GGAGAGAAGA AAGAACAAGC AGGCCCGACG TCTGAGATTA CTGCAGTAGA GTCCATACCA
    GAGCTTCCTC TGCCTCCATC CCCTACTGCT ATTTCTCCAC TTGCTCGAAG TACAATTACT
    TCTCCCACCT CTGTTGCTCT TAGTAGCCAA CCAATATTCA CCACTGCAAT TGATGACAGA
    TGTGAACTCT CTTCCTCAAA AGAAGATACA ATTCCTATTC CCAGCCCCAC ATCTTGCACA
    GAAGCATCAG ATCCTTTACC AACAGATGAA ATTGATGATG ATATATGCAA GAAATCTTGT
    AGTGTAGCAC CAAATGATAT TCCACTGATT TCTAGTACTA ACCTAATTAA TGAAATGAAT
    GGAGTTAATG AAAAATTACC AGCCACGGAA AGCATTGTGG AAATAGTAAA ACAGGAGGTG
    TTACCGCTGA CTCTTGAATT GGAGATTTTG GAAAATCCCC CTGAAGAAAT CAAAGTGGAG
    TGTGTCCCAA CTCCCATCAC CCCTTCGATA GTTCCCTCCT CTTCTCCGTC TCCTCCAGCT
    CCTCCAGCTT CTCCTTCTCC CACACCAGTT ATCGTTCCTG CTGCTGCCAC CAGTGTTAAT
    ACTGGTCGTG GTTCCACCGG AGTCCAGAGA GCCTTCGAAG AGGGCGAGAA CATAAGAACT
    TGCCTTAGTG AAGATGCAAA AGAGATTCAG AACAAAGTAG AGGTAGAAGC AGATGGGCAA
    ACAGAAGAGA TTGTGGATTC TCAGAACTTA AGTTCAAGAA AGAGCCCAGT CCCAGCTCAG
    ACAGCTATAC CTGCACCAAA GATGTGGAAG AAACCAAAAG ACCGGACCCG AGCCACTGAA
    GAGTCGTTAG AGGCAGAATC AGAGCCTAAA GCTGAAGAGG AGCTTTCAGG TGACAAAATA
    CTTGAATCTG AACAGGATAA AATGAGCCAA GGGTTTCATC CTGAAAGAGA CCCCTCTGAC
    CTAAAAAACG TGAAAACTGT GGAAGAAAAT GGAGAAGAAG CTGAGCCTGT ACGTAATGGT
    GCTGAGAGTA TTTCTGAGGG TGAAGGAATA GATGCTAATT CAGGCTCCAC TGATAGTTCT
    GGTGAAGGGG TCACATTCCC ATTTAAGTCA GAATCCTGGA AGCCTCCTGA TTCGGAAGGC
    AAGAAGCAGT ATGACAGGGA GTTTTTGCTG GATTTCCAAT TCATGCCAGC GTGTATTCAG
    AAACCAGAGG GCCTGCCTCC CATCAGTGAT GTGGTTCTTG ACAAGATCAA TCAACCAAAA
    TTGCCAATGC GAACTCTGGA TCCTCGGATT TTGCCTCGAG GACCAGACTT TACTCCAGCC
    TTTGCAGATT TTGGAAGGCA AGCCCCTGGT GGAAGAGGTG TACCTGTTTG CAAAGTGCAG
    AGCAGGCATG GATTGCCAAT TCTGGAACAG AGCAAAGCCC CAGCTTCCAC TCCACTGGCG
    ATGTCGCACC CACCCCTGAA GAGCCTGCCT CTAGGGCTGT TGAATGTTGG AGCACGGAGA
    TCTCAGCCTG GCCAGAGGAG AGAGCCCAGG AAGATCATCA CGGTCTCCGT GAAAGAAGAC
    GTGCACCTGA AGAAGGCAGA GAACGCCTGG AAGCCCAGCC AGAGGCGGGA TAGCCCAGCC
    GAGGACCCCG AGAACATCAA GACCCAGGAA CTTTTTAGAA AAGTTCGAAG TATCTTAAAT
    AAATTGACAC CACAGATGTT CAACCAACTG ATGAAGCAAG TGTCAGGACT TACTGTTGAC
    ACAGAGGAGC GGCTGAAAGG AGTCATTGAC CTGGTCTTTG AGAAGGCTAT TGACGAACCC
    AGTTTCTCTG TGGCTTACGC AAACATGTGT CGATGTCTAG TAACGCTGAA AGTACCCATG
    GCAGACAAGC CTGGTAACAC AGTGAACTTC CGGAAGCTAC TACTGAACCG TTGCCAGAAG
    GAATTTGAAA AAGATAAAGC CGATGATGAT GTCTTTGAGA AGAAGCAGAA AGAACTCGAG
    GCTGCCAGTG CTCCAGAGGA GAGGACAAGG CTTCATGATG AACTGGAAGA AGCTAAAGAT
    AAAGCCCGGC GGAGATCCAT TGGCAACATC AAGTTCATTG GAGAACTCTT TAAACTCAAA
    ATGCTGACTG AAGCCATCAT GCATGACTGT GTGGTGAAGC TGCTGAAGAA CCACGATGAA
    GAATCCCTCG AGTGTCTGTG TCGCCTGCTC ACCACAATTG GCAAAGACCT GGACTTTGAG
    AAAGCAAAGC CACGTATGGA CCAGTATTTT AATCAGATGG AGAAAATTGT AAAAGAAAGA
    AAAACCTCAT CTAGGATTCG ATTCATGCTT CAGGACGTAA TAGATCTAAG GCTGTGTAAT
    TGGGTATCAC GACGAGCAGA TCAGGGGCCT AAAACTATTG AACAGATTCA CAAAGAAGCT
    AAAATAGAAG AACAGGAAGA GCAGAGGAAG GTCCAACAAC TCATGACCAA AGAGAAGAGA
    AGACCAGGTG TCCAGAGAGT GGATGAAGGT GGGTGGAACA CTGTTCAAGG GGCCAAGAAT
    AGTCGGGTAT TGGACCCCTC AAAATTTCTG AAAATCACTA AGCCCACAAT TGATGAGAAA
    ATTCAGCTGG TACCTAAAGC GCAGCTGGGC AGCTGGGGAA AGGGCAGCAG TGGTGGAGCC
    AAGGCGAGCG AGGCCGATGC CTTAAGATCA AGTGCTTCCA GTTTAAACAG ATTCTCTGCT
    TTGCAACCTC CAGCACCTTC AGGGTCTGCG TCATCCACAC CTCTAGAGTT TGATTCCCGG
    CGGACCTTAA CTAGTCGAGG AAGCACGGGC AGGGAGAAGA ATGACAAGCC CCTCCAGCCT
    GCAGCAGCTC GGCCGAACAC TTTCATGAGG GGTGGCAGCA GTAAAGACCT GTTAGACAAT
    CAGTCTCAGG AGGAGCAGCG GAGGGAGATG CTGGAGACCG TGAAGCAGCT CACAGGAGGC
    ATGGACGTGG AGAGGAGCAG CGCTGAGGCG GAGCGAAGCA GGACCAGGGA GCCAGTGACA
    AAACCGGAAA TTCCAGTAAC ATCAGCCTCT GACAAGCCCG CGTTGTCAGA GGAAGAGATG
    GAGAGGAAGT CCAAATCTAT CATTGATGAG TTTCTACACA TTAATGATTT TAAGGAAGCC
    ATGCAGTGTG TGGAAGAGCT GAACGCCCAG GGCCTGCAAC ATGTGTTCGT GAGGACGGGA
    GTGGAGTCCA CCCTGGAAAG GAGCCAGATC ACCCGGGATC ACATGGGCCA GTTACTGTAT
    CAGCTGGTGC AGTCAGAAAA GCTCAGCAAA CAGGACTTTT TCAGAGGTTT TTCAGAAACA
    TTGGAATTGG CAGATGACAT GGCCATTGAT ATTCCCCATA TTTGGTTGTA CCTTGCTGAA
    CTGGTGACTC CCATGTTAAA AGAAGGCGGA ATCTCCATGA GACAACTCAT CACAGAATTT
    TGCAAACCTT TACTTCCTGT TGGAAGAGCT GGAATCTTGA TTTCTGAAAT ATTGCACCTT
    CTATGCAAAC AAATGAGCCA TAAGAAAGTG GGAGCCCTAT GGAGGGAGGC TGACCTCAGC
    TGGAAGGACT TTTTACCAGA AGGGGAAGAT GTACATAATT TTCTCTTGGA GCAGAAGTTG
    GACTTTTTAG AGTCTGACAG TTCCTGTTCC TCCGAAGCAC TTTTGAAGAA AGAACTCTCT
    GCCGAAGAGC TGTATAAGCG ACTCGAGAAA CTCATTATTG AGGACAAGGC AAATGATGAA
    CAGATCTTTG ACTGGGTAGA GGCTAATCTA GATGAAAGCC AGATGAGTTC ACCTACATTC
    CTTAGAGCTT TAATGACAGC CGTCTGTAAA GCAGCTATTA TAGCCGACTG TTCGACCTTC
    CGAGTGGACA CTGCTGTTAT CAAGCAGAGA GTGCCGATCT TACTCAAGTA CCTAGACTCA
    GATACAGAGA AGGAACTGCA AGCACTTTAT GCACTACAAG CATCAATAGT AAAACTTGAT
    CAACCTGCCA ATTTGCTACG GATGTTTTTT GATTGCCTGT ATGACGAGGA GGTGATCTCT
    GAGGATGCCT TCTACAAGTG GGAGAGCAGC AAGGACCCGG CAGAGCAGAA TGGGAAGGGT
    GTGGCCCTGA AGTCTGTCAC GGCGTTCTTC ACATGGCTGC GGGAAGCAGA AGAGGAGTCT
    GAGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88096
    Clone ID Related Accession (Same CDS sequence) XM_015460886.2 , XM_024983008.1 , XM_015460886.1 , XM_015462482.1 , XM_024983012.1
    Accession Version XM_015460886.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTTTCCAGA GGCCTCAAAT ACAGCCTCCC 
    AGAGCCACCA TCCCGAACAG CAGCCCTTCC ATTCGACCCG GTGCACAGAC ACCCACTGCA
    GTGTATCAGG CCAATCAGCA CATCATGATG GTTAACCATC TGCCCATGCC GTACCCAGTG
    CCCCAGGGTC CTCAGTACTG TATACCACAG TACCGTCACA GTGGCCCTCC GTATGTTGGG
    CCACCACAAC AGTACCCGGT TCAACCACCA GGACCAGGTC CTTTTTATCC TGGGCCAGGA
    CCTGGGGACT TCCCCAGTGC TTACGGAACG CCTTTTTACC CAAGTCAGCC AGTGTACCAG
    TCAGCACCTA TCATAGTGCC TACGCAGCAG CAGCCCCCTC CAGCCAAGAG AGAGAAAAAA
    ACAATAAGAA TTCGGGATCC AAACCAGGGA GGTAAAGACA TAACAGAGGA GATTATGTCT
    GGAGGTGGCA GCCGAAATCC TACTCCACCC ATAGGGAGAC CCACTTCCAC ACCTACTCCT
    CCTCAGCAGC TGCCCAGCCA GGTCCCCGAG CACAGCCCTG TGGTCTATGG GACTGTGGAG
    AGCGCTCATC TTGCTGCCAG CACCCCTGTC ACTGCAGCGA GCGACCAGAA GCAAGAAGAG
    AAGCCAAAAC CAGATCCAGT GTTAAAGACT CCTTCCCCAG TCCTTAGGCT AGCCCTCAGT
    GGAGAGAAGA AAGAACAAGC AGGCCCGACG TCTGAGATTA CTGCAGTAGA GTCCATACCA
    GAGCTTCCTC TGCCTCCATC CCCTACTGCT ATTTCTCCAC TTGCTCGAAG TACAATTACT
    TCTCCCACCT CTGTTGCTCT TAGTAGCCAA CCAATATTCA CCACTGCAAT TGATGACAGA
    TGTGAACTCT CTTCCTCAAA AGAAGATACA ATTCCTATTC CCAGCCCCAC ATCTTGCACA
    GAAGCATCAG ATCCTTTACC AACAGATGAA ATTGATGATG ATATATGCAA GAAATCTTGT
    AGTGTAGCAC CAAATGATAT TCCACTGATT TCTAGTACTA ACCTAATTAA TGAAATGAAT
    GGAGTTAATG AAAAATTACC AGCCACGGAA AGCATTGTGG AAATAGTAAA ACAGGAGGTG
    TTACCGCTGA CTCTTGAATT GGAGATTTTG GAAAATCCCC CTGAAGAAAT CAAAGTGGAG
    TGTGTCCCAA CTCCCATCAC CCCTTCGATA GTTCCCTCCT CTTCTCCGTC TCCTCCAGCT
    CCTCCAGCTT CTCCTTCTCC CACACCAGTT ATCGTTCCTG CTGCTGCCAC CAGTGTTAAT
    ACTGGTCGTG GTTCCACCGG AGTCCAGAGA GCCTTCGAAG AGGGCGAGAA CATAAGAACT
    TGCCTTAGTG AAGATGCAAA AGAGATTCAG AACAAAGTAG AGGTAGAAGC AGATGGGCAA
    ACAGAAGAGA TTGTGGATTC TCAGAACTTA AGTTCAAGAA AGAGCCCAGT CCCAGCTCAG
    ACAGCTATAC CTGCACCAAA GATGTGGAAG AAACCAAAAG ACCGGACCCG AGCCACTGAA
    GAGTCGTTAG AGGCAGAATC AGAGCCTAAA GCTGAAGAGG AGCTTTCAGG TGACAAAATA
    CTTGAATCTG AACAGGATAA AATGAGCCAA GGGTTTCATC CTGAAAGAGA CCCCTCTGAC
    CTAAAAAACG TGAAAACTGT GGAAGAAAAT GGAGAAGAAG CTGAGCCTGT ACGTAATGGT
    GCTGAGAGTA TTTCTGAGGG TGAAGGAATA GATGCTAATT CAGGCTCCAC TGATAGTTCT
    GGTGAAGGGG TCACATTCCC ATTTAAGTCA GAATCCTGGA AGCCTCCTGA TTCGGAAGGC
    AAGAAGCAGT ATGACAGGGA GTTTTTGCTG GATTTCCAAT TCATGCCAGC GTGTATTCAG
    AAACCAGAGG GCCTGCCTCC CATCAGTGAT GTGGTTCTTG ACAAGATCAA TCAACCAAAA
    TTGCCAATGC GAACTCTGGA TCCTCGGATT TTGCCTCGAG GACCAGACTT TACTCCAGCC
    TTTGCAGATT TTGGAAGGCA AGCCCCTGGT GGAAGAGGTG TACCTGTTTG CAAAGTGCAG
    AGCAGGCATG GATTGCCAAT TCTGGAACAG AGCAAAGCCC CAGCTTCCAC TCCACTGGCG
    ATGTCGCACC CACCCCTGAA GAGCCTGCCT CTAGGGCTGT TGAATGTTGG AGCACGGAGA
    TCTCAGCCTG GCCAGAGGAG AGAGCCCAGG AAGATCATCA CGGTCTCCGT GAAAGAAGAC
    GTGCACCTGA AGAAGGCAGA GAACGCCTGG AAGCCCAGCC AGAGGCGGGA TAGCCCAGCC
    GAGGACCCCG AGAACATCAA GACCCAGGAA CTTTTTAGAA AAGTTCGAAG TATCTTAAAT
    AAATTGACAC CACAGATGTT CAACCAACTG ATGAAGCAAG TGTCAGGACT TACTGTTGAC
    ACAGAGGAGC GGCTGAAAGG AGTCATTGAC CTGGTCTTTG AGAAGGCTAT TGACGAACCC
    AGTTTCTCTG TGGCTTACGC AAACATGTGT CGATGTCTAG TAACGCTGAA AGTACCCATG
    GCAGACAAGC CTGGTAACAC AGTGAACTTC CGGAAGCTAC TACTGAACCG TTGCCAGAAG
    GAATTTGAAA AAGATAAAGC CGATGATGAT GTCTTTGAGA AGAAGCAGAA AGAACTCGAG
    GCTGCCAGTG CTCCAGAGGA GAGGACAAGG CTTCATGATG AACTGGAAGA AGCTAAAGAT
    AAAGCCCGGC GGAGATCCAT TGGCAACATC AAGTTCATTG GAGAACTCTT TAAACTCAAA
    ATGCTGACTG AAGCCATCAT GCATGACTGT GTGGTGAAGC TGCTGAAGAA CCACGATGAA
    GAATCCCTCG AGTGTCTGTG TCGCCTGCTC ACCACAATTG GCAAAGACCT GGACTTTGAG
    AAAGCAAAGC CACGTATGGA CCAGTATTTT AATCAGATGG AGAAAATTGT AAAAGAAAGA
    AAAACCTCAT CTAGGATTCG ATTCATGCTT CAGGACGTAA TAGATCTAAG GCTGTGTAAT
    TGGGTATCAC GACGAGCAGA TCAGGGGCCT AAAACTATTG AACAGATTCA CAAAGAAGCT
    AAAATAGAAG AACAGGAAGA GCAGAGGAAG GTCCAACAAC TCATGACCAA AGAGAAGAGA
    AGACCAGGTG TCCAGAGAGT GGATGAAGGT GGGTGGAACA CTGTTCAAGG GGCCAAGAAT
    AGTCGGGTAT TGGACCCCTC AAAATTTCTG AAAATCACTA AGCCCACAAT TGATGAGAAA
    ATTCAGCTGG TACCTAAAGC GCAGCTGGGC AGCTGGGGAA AGGGCAGCAG TGGTGGAGCC
    AAGGCGAGCG AGGCCGATGC CTTAAGATCA AGTGCTTCCA GTTTAAACAG ATTCTCTGCT
    TTGCAACCTC CAGCACCTTC AGGGTCTGCG TCATCCACAC CTCTAGAGTT TGATTCCCGG
    CGGACCTTAA CTAGTCGAGG AAGCACGGGC AGGGAGAAGA ATGACAAGCC CCTCCAGCCT
    GCAGCAGCTC GGCCGAACAC TTTCATGAGG GGTGGCAGCA GTAAAGACCT GTTAGACAAT
    CAGTCTCAGG AGGAGCAGCG GAGGGAGATG CTGGAGACCG TGAAGCAGCT CACAGGAGGC
    ATGGACGTGG AGAGGAGCAG CGCTGAGGCG GAGCGAAGCA GGACCAGGGA GCCAGTGACA
    AAACCGGAAA TTCCAGTAAC ATCAGCCTCT GACAAGCCCG CGTTGTCAGA GGAAGAGATG
    GAGAGGAAGT CCAAATCTAT CATTGATGAG TTTCTACACA TTAATGATTT TAAGGAAGCC
    ATGCAGTGTG TGGAAGAGCT GAACGCCCAG GGCCTGCAAC ATGTGTTCGT GAGGACGGGA
    GTGGAGTCCA CCCTGGAAAG GAGCCAGATC ACCCGGGATC ACATGGGCCA GTTACTGTAT
    CAGCTGGTGC AGTCAGAAAA GCTCAGCAAA CAGGACTTTT TCAGAGGTTT TTCAGAAACA
    TTGGAATTGG CAGATGACAT GGCCATTGAT ATTCCCCATA TTTGGTTGTA CCTTGCTGAA
    CTGGTGACTC CCATGTTAAA AGAAGGCGGA ATCTCCATGA GACAACTCAT CACAGAATTT
    TGCAAACCTT TACTTCCTGT TGGAAGAGCT GGAATCTTGA TTTCTGAAAT ATTGCACCTT
    CTATGCAAAC AAATGAGCCA TAAGAAAGTG GGAGCCCTAT GGAGGGAGGC TGACCTCAGC
    TGGAAGGACT TTTTACCAGA AGGGGAAGAT GTACATAATT TTCTCTTGGA GCAGAAGTTG
    GACTTTTTAG AGTCTGACAG TTCCTGTTCC TCCGAAGCAC TTTTGAAGAA AGAACTCTCT
    GCCGAAGAGC TGTATAAGCG ACTCGAGAAA CTCATTATTG AGGACAAGGC AAATGATGAA
    CAGATCTTTG ACTGGGTAGA GGCTAATCTA GATGAAAGCC AGATGAGTTC ACCTACATTC
    CTTAGAGCTT TAATGACAGC CGTCTGTAAA GCAGCTATTA TAGCCGACTG TTCGACCTTC
    CGAGTGGACA CTGCTGTTAT CAAGCAGAGA GTGCCGATCT TACTCAAGTA CCTAGACTCA
    GATACAGAGA AGGAACTGCA AGCACTTTAT GCACTACAAG CATCAATAGT AAAACTTGAT
    CAACCTGCCA ATTTGCTACG GATGTTTTTT GATTGCCTGT ATGACGAGGA GGTGATCTCT
    GAGGATGCCT TCTACAAGTG GGAGAGCAGC AAGGACCCGG CAGAGCAGAA TGGGAAGGGT
    GTGGCCCTGA AGTCTGTCAC GGCGTTCTTC ACATGGCTGC GGGAAGCAGA AGAGGAGTCT
    GAGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316372.1
    CDS263..5134
    Translation

    Target ORF information:

    RefSeq Version XM_015460886.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTTTCCAGA GGCCTCAAAT ACAGCCTCCC 
    AGAGCCACCA TCCCGAACAG CAGCCCTTCC ATTCGACCCG GTGCACAGAC ACCCACTGCA
    GTGTATCAGG CCAATCAGCA CATCATGATG GTTAACCATC TGCCCATGCC GTACCCAGTG
    CCCCAGGGTC CTCAGTACTG TATACCACAG TACCGTCACA GTGGCCCTCC GTATGTTGGG
    CCACCACAAC AGTACCCGGT TCAACCACCA GGACCAGGTC CTTTTTATCC TGGGCCAGGA
    CCTGGGGACT TCCCCAGTGC TTACGGAACG CCTTTTTACC CAAGTCAGCC AGTGTACCAG
    TCAGCACCTA TCATAGTGCC TACGCAGCAG CAGCCCCCTC CAGCCAAGAG AGAGAAAAAA
    ACAATAAGAA TTCGGGATCC AAACCAGGGA GGTAAAGACA TAACAGAGGA GATTATGTCT
    GGAGGTGGCA GCCGAAATCC TACTCCACCC ATAGGGAGAC CCACTTCCAC ACCTACTCCT
    CCTCAGCAGC TGCCCAGCCA GGTCCCCGAG CACAGCCCTG TGGTCTATGG GACTGTGGAG
    AGCGCTCATC TTGCTGCCAG CACCCCTGTC ACTGCAGCGA GCGACCAGAA GCAAGAAGAG
    AAGCCAAAAC CAGATCCAGT GTTAAAGACT CCTTCCCCAG TCCTTAGGCT AGCCCTCAGT
    GGAGAGAAGA AAGAACAAGC AGGCCCGACG TCTGAGATTA CTGCAGTAGA GTCCATACCA
    GAGCTTCCTC TGCCTCCATC CCCTACTGCT ATTTCTCCAC TTGCTCGAAG TACAATTACT
    TCTCCCACCT CTGTTGCTCT TAGTAGCCAA CCAATATTCA CCACTGCAAT TGATGACAGA
    TGTGAACTCT CTTCCTCAAA AGAAGATACA ATTCCTATTC CCAGCCCCAC ATCTTGCACA
    GAAGCATCAG ATCCTTTACC AACAGATGAA ATTGATGATG ATATATGCAA GAAATCTTGT
    AGTGTAGCAC CAAATGATAT TCCACTGATT TCTAGTACTA ACCTAATTAA TGAAATGAAT
    GGAGTTAATG AAAAATTACC AGCCACGGAA AGCATTGTGG AAATAGTAAA ACAGGAGGTG
    TTACCGCTGA CTCTTGAATT GGAGATTTTG GAAAATCCCC CTGAAGAAAT CAAAGTGGAG
    TGTGTCCCAA CTCCCATCAC CCCTTCGATA GTTCCCTCCT CTTCTCCGTC TCCTCCAGCT
    CCTCCAGCTT CTCCTTCTCC CACACCAGTT ATCGTTCCTG CTGCTGCCAC CAGTGTTAAT
    ACTGGTCGTG GTTCCACCGG AGTCCAGAGA GCCTTCGAAG AGGGCGAGAA CATAAGAACT
    TGCCTTAGTG AAGATGCAAA AGAGATTCAG AACAAAGTAG AGGTAGAAGC AGATGGGCAA
    ACAGAAGAGA TTGTGGATTC TCAGAACTTA AGTTCAAGAA AGAGCCCAGT CCCAGCTCAG
    ACAGCTATAC CTGCACCAAA GATGTGGAAG AAACCAAAAG ACCGGACCCG AGCCACTGAA
    GAGTCGTTAG AGGCAGAATC AGAGCCTAAA GCTGAAGAGG AGCTTTCAGG TGACAAAATA
    CTTGAATCTG AACAGGATAA AATGAGCCAA GGGTTTCATC CTGAAAGAGA CCCCTCTGAC
    CTAAAAAACG TGAAAACTGT GGAAGAAAAT GGAGAAGAAG CTGAGCCTGT ACGTAATGGT
    GCTGAGAGTA TTTCTGAGGG TGAAGGAATA GATGCTAATT CAGGCTCCAC TGATAGTTCT
    GGTGAAGGGG TCACATTCCC ATTTAAGTCA GAATCCTGGA AGCCTCCTGA TTCGGAAGGC
    AAGAAGCAGT ATGACAGGGA GTTTTTGCTG GATTTCCAAT TCATGCCAGC GTGTATTCAG
    AAACCAGAGG GCCTGCCTCC CATCAGTGAT GTGGTTCTTG ACAAGATCAA TCAACCAAAA
    TTGCCAATGC GAACTCTGGA TCCTCGGATT TTGCCTCGAG GACCAGACTT TACTCCAGCC
    TTTGCAGATT TTGGAAGGCA AGCCCCTGGT GGAAGAGGTG TACCTGTTTG CAAAGTGCAG
    AGCAGGCATG GATTGCCAAT TCTGGAACAG AGCAAAGCCC CAGCTTCCAC TCCACTGGCG
    ATGTCGCACC CACCCCTGAA GAGCCTGCCT CTAGGGCTGT TGAATGTTGG AGCACGGAGA
    TCTCAGCCTG GCCAGAGGAG AGAGCCCAGG AAGATCATCA CGGTCTCCGT GAAAGAAGAC
    GTGCACCTGA AGAAGGCAGA GAACGCCTGG AAGCCCAGCC AGAGGCGGGA TAGCCCAGCC
    GAGGACCCCG AGAACATCAA GACCCAGGAA CTTTTTAGAA AAGTTCGAAG TATCTTAAAT
    AAATTGACAC CACAGATGTT CAACCAACTG ATGAAGCAAG TGTCAGGACT TACTGTTGAC
    ACAGAGGAGC GGCTGAAAGG AGTCATTGAC CTGGTCTTTG AGAAGGCTAT TGACGAACCC
    AGTTTCTCTG TGGCTTACGC AAACATGTGT CGATGTCTAG TAACGCTGAA AGTACCCATG
    GCAGACAAGC CTGGTAACAC AGTGAACTTC CGGAAGCTAC TACTGAACCG TTGCCAGAAG
    GAATTTGAAA AAGATAAAGC CGATGATGAT GTCTTTGAGA AGAAGCAGAA AGAACTCGAG
    GCTGCCAGTG CTCCAGAGGA GAGGACAAGG CTTCATGATG AACTGGAAGA AGCTAAAGAT
    AAAGCCCGGC GGAGATCCAT TGGCAACATC AAGTTCATTG GAGAACTCTT TAAACTCAAA
    ATGCTGACTG AAGCCATCAT GCATGACTGT GTGGTGAAGC TGCTGAAGAA CCACGATGAA
    GAATCCCTCG AGTGTCTGTG TCGCCTGCTC ACCACAATTG GCAAAGACCT GGACTTTGAG
    AAAGCAAAGC CACGTATGGA CCAGTATTTT AATCAGATGG AGAAAATTGT AAAAGAAAGA
    AAAACCTCAT CTAGGATTCG ATTCATGCTT CAGGACGTAA TAGATCTAAG GCTGTGTAAT
    TGGGTATCAC GACGAGCAGA TCAGGGGCCT AAAACTATTG AACAGATTCA CAAAGAAGCT
    AAAATAGAAG AACAGGAAGA GCAGAGGAAG GTCCAACAAC TCATGACCAA AGAGAAGAGA
    AGACCAGGTG TCCAGAGAGT GGATGAAGGT GGGTGGAACA CTGTTCAAGG GGCCAAGAAT
    AGTCGGGTAT TGGACCCCTC AAAATTTCTG AAAATCACTA AGCCCACAAT TGATGAGAAA
    ATTCAGCTGG TACCTAAAGC GCAGCTGGGC AGCTGGGGAA AGGGCAGCAG TGGTGGAGCC
    AAGGCGAGCG AGGCCGATGC CTTAAGATCA AGTGCTTCCA GTTTAAACAG ATTCTCTGCT
    TTGCAACCTC CAGCACCTTC AGGGTCTGCG TCATCCACAC CTCTAGAGTT TGATTCCCGG
    CGGACCTTAA CTAGTCGAGG AAGCACGGGC AGGGAGAAGA ATGACAAGCC CCTCCAGCCT
    GCAGCAGCTC GGCCGAACAC TTTCATGAGG GGTGGCAGCA GTAAAGACCT GTTAGACAAT
    CAGTCTCAGG AGGAGCAGCG GAGGGAGATG CTGGAGACCG TGAAGCAGCT CACAGGAGGC
    ATGGACGTGG AGAGGAGCAG CGCTGAGGCG GAGCGAAGCA GGACCAGGGA GCCAGTGACA
    AAACCGGAAA TTCCAGTAAC ATCAGCCTCT GACAAGCCCG CGTTGTCAGA GGAAGAGATG
    GAGAGGAAGT CCAAATCTAT CATTGATGAG TTTCTACACA TTAATGATTT TAAGGAAGCC
    ATGCAGTGTG TGGAAGAGCT GAACGCCCAG GGCCTGCAAC ATGTGTTCGT GAGGACGGGA
    GTGGAGTCCA CCCTGGAAAG GAGCCAGATC ACCCGGGATC ACATGGGCCA GTTACTGTAT
    CAGCTGGTGC AGTCAGAAAA GCTCAGCAAA CAGGACTTTT TCAGAGGTTT TTCAGAAACA
    TTGGAATTGG CAGATGACAT GGCCATTGAT ATTCCCCATA TTTGGTTGTA CCTTGCTGAA
    CTGGTGACTC CCATGTTAAA AGAAGGCGGA ATCTCCATGA GACAACTCAT CACAGAATTT
    TGCAAACCTT TACTTCCTGT TGGAAGAGCT GGAATCTTGA TTTCTGAAAT ATTGCACCTT
    CTATGCAAAC AAATGAGCCA TAAGAAAGTG GGAGCCCTAT GGAGGGAGGC TGACCTCAGC
    TGGAAGGACT TTTTACCAGA AGGGGAAGAT GTACATAATT TTCTCTTGGA GCAGAAGTTG
    GACTTTTTAG AGTCTGACAG TTCCTGTTCC TCCGAAGCAC TTTTGAAGAA AGAACTCTCT
    GCCGAAGAGC TGTATAAGCG ACTCGAGAAA CTCATTATTG AGGACAAGGC AAATGATGAA
    CAGATCTTTG ACTGGGTAGA GGCTAATCTA GATGAAAGCC AGATGAGTTC ACCTACATTC
    CTTAGAGCTT TAATGACAGC CGTCTGTAAA GCAGCTATTA TAGCCGACTG TTCGACCTTC
    CGAGTGGACA CTGCTGTTAT CAAGCAGAGA GTGCCGATCT TACTCAAGTA CCTAGACTCA
    GATACAGAGA AGGAACTGCA AGCACTTTAT GCACTACAAG CATCAATAGT AAAACTTGAT
    CAACCTGCCA ATTTGCTACG GATGTTTTTT GATTGCCTGT ATGACGAGGA GGTGATCTCT
    GAGGATGCCT TCTACAAGTG GGAGAGCAGC AAGGACCCGG CAGAGCAGAA TGGGAAGGGT
    GTGGCCCTGA AGTCTGTCAC GGCGTTCTTC ACATGGCTGC GGGAAGCAGA AGAGGAGTCT
    GAGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88096
    Clone ID Related Accession (Same CDS sequence) XM_015460886.2 , XM_024983008.1 , XM_015460886.1 , XM_015462482.1 , XM_024983012.1
    Accession Version XM_015462482.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTTTCCAGA GGCCTCAAAT ACAGCCTCCC 
    AGAGCCACCA TCCCGAACAG CAGCCCTTCC ATTCGACCCG GTGCACAGAC ACCCACTGCA
    GTGTATCAGG CCAATCAGCA CATCATGATG GTTAACCATC TGCCCATGCC GTACCCAGTG
    CCCCAGGGTC CTCAGTACTG TATACCACAG TACCGTCACA GTGGCCCTCC GTATGTTGGG
    CCACCACAAC AGTACCCGGT TCAACCACCA GGACCAGGTC CTTTTTATCC TGGGCCAGGA
    CCTGGGGACT TCCCCAGTGC TTACGGAACG CCTTTTTACC CAAGTCAGCC AGTGTACCAG
    TCAGCACCTA TCATAGTGCC TACGCAGCAG CAGCCCCCTC CAGCCAAGAG AGAGAAAAAA
    ACAATAAGAA TTCGGGATCC AAACCAGGGA GGTAAAGACA TAACAGAGGA GATTATGTCT
    GGAGGTGGCA GCCGAAATCC TACTCCACCC ATAGGGAGAC CCACTTCCAC ACCTACTCCT
    CCTCAGCAGC TGCCCAGCCA GGTCCCCGAG CACAGCCCTG TGGTCTATGG GACTGTGGAG
    AGCGCTCATC TTGCTGCCAG CACCCCTGTC ACTGCAGCGA GCGACCAGAA GCAAGAAGAG
    AAGCCAAAAC CAGATCCAGT GTTAAAGACT CCTTCCCCAG TCCTTAGGCT AGCCCTCAGT
    GGAGAGAAGA AAGAACAAGC AGGCCCGACG TCTGAGATTA CTGCAGTAGA GTCCATACCA
    GAGCTTCCTC TGCCTCCATC CCCTACTGCT ATTTCTCCAC TTGCTCGAAG TACAATTACT
    TCTCCCACCT CTGTTGCTCT TAGTAGCCAA CCAATATTCA CCACTGCAAT TGATGACAGA
    TGTGAACTCT CTTCCTCAAA AGAAGATACA ATTCCTATTC CCAGCCCCAC ATCTTGCACA
    GAAGCATCAG ATCCTTTACC AACAGATGAA ATTGATGATG ATATATGCAA GAAATCTTGT
    AGTGTAGCAC CAAATGATAT TCCACTGATT TCTAGTACTA ACCTAATTAA TGAAATGAAT
    GGAGTTAATG AAAAATTACC AGCCACGGAA AGCATTGTGG AAATAGTAAA ACAGGAGGTG
    TTACCGCTGA CTCTTGAATT GGAGATTTTG GAAAATCCCC CTGAAGAAAT CAAAGTGGAG
    TGTGTCCCAA CTCCCATCAC CCCTTCGATA GTTCCCTCCT CTTCTCCGTC TCCTCCAGCT
    CCTCCAGCTT CTCCTTCTCC CACACCAGTT ATCGTTCCTG CTGCTGCCAC CAGTGTTAAT
    ACTGGTCGTG GTTCCACCGG AGTCCAGAGA GCCTTCGAAG AGGGCGAGAA CATAAGAACT
    TGCCTTAGTG AAGATGCAAA AGAGATTCAG AACAAAGTAG AGGTAGAAGC AGATGGGCAA
    ACAGAAGAGA TTGTGGATTC TCAGAACTTA AGTTCAAGAA AGAGCCCAGT CCCAGCTCAG
    ACAGCTATAC CTGCACCAAA GATGTGGAAG AAACCAAAAG ACCGGACCCG AGCCACTGAA
    GAGTCGTTAG AGGCAGAATC AGAGCCTAAA GCTGAAGAGG AGCTTTCAGG TGACAAAATA
    CTTGAATCTG AACAGGATAA AATGAGCCAA GGGTTTCATC CTGAAAGAGA CCCCTCTGAC
    CTAAAAAACG TGAAAACTGT GGAAGAAAAT GGAGAAGAAG CTGAGCCTGT ACGTAATGGT
    GCTGAGAGTA TTTCTGAGGG TGAAGGAATA GATGCTAATT CAGGCTCCAC TGATAGTTCT
    GGTGAAGGGG TCACATTCCC ATTTAAGTCA GAATCCTGGA AGCCTCCTGA TTCGGAAGGC
    AAGAAGCAGT ATGACAGGGA GTTTTTGCTG GATTTCCAAT TCATGCCAGC GTGTATTCAG
    AAACCAGAGG GCCTGCCTCC CATCAGTGAT GTGGTTCTTG ACAAGATCAA TCAACCAAAA
    TTGCCAATGC GAACTCTGGA TCCTCGGATT TTGCCTCGAG GACCAGACTT TACTCCAGCC
    TTTGCAGATT TTGGAAGGCA AGCCCCTGGT GGAAGAGGTG TACCTGTTTG CAAAGTGCAG
    AGCAGGCATG GATTGCCAAT TCTGGAACAG AGCAAAGCCC CAGCTTCCAC TCCACTGGCG
    ATGTCGCACC CACCCCTGAA GAGCCTGCCT CTAGGGCTGT TGAATGTTGG AGCACGGAGA
    TCTCAGCCTG GCCAGAGGAG AGAGCCCAGG AAGATCATCA CGGTCTCCGT GAAAGAAGAC
    GTGCACCTGA AGAAGGCAGA GAACGCCTGG AAGCCCAGCC AGAGGCGGGA TAGCCCAGCC
    GAGGACCCCG AGAACATCAA GACCCAGGAA CTTTTTAGAA AAGTTCGAAG TATCTTAAAT
    AAATTGACAC CACAGATGTT CAACCAACTG ATGAAGCAAG TGTCAGGACT TACTGTTGAC
    ACAGAGGAGC GGCTGAAAGG AGTCATTGAC CTGGTCTTTG AGAAGGCTAT TGACGAACCC
    AGTTTCTCTG TGGCTTACGC AAACATGTGT CGATGTCTAG TAACGCTGAA AGTACCCATG
    GCAGACAAGC CTGGTAACAC AGTGAACTTC CGGAAGCTAC TACTGAACCG TTGCCAGAAG
    GAATTTGAAA AAGATAAAGC CGATGATGAT GTCTTTGAGA AGAAGCAGAA AGAACTCGAG
    GCTGCCAGTG CTCCAGAGGA GAGGACAAGG CTTCATGATG AACTGGAAGA AGCTAAAGAT
    AAAGCCCGGC GGAGATCCAT TGGCAACATC AAGTTCATTG GAGAACTCTT TAAACTCAAA
    ATGCTGACTG AAGCCATCAT GCATGACTGT GTGGTGAAGC TGCTGAAGAA CCACGATGAA
    GAATCCCTCG AGTGTCTGTG TCGCCTGCTC ACCACAATTG GCAAAGACCT GGACTTTGAG
    AAAGCAAAGC CACGTATGGA CCAGTATTTT AATCAGATGG AGAAAATTGT AAAAGAAAGA
    AAAACCTCAT CTAGGATTCG ATTCATGCTT CAGGACGTAA TAGATCTAAG GCTGTGTAAT
    TGGGTATCAC GACGAGCAGA TCAGGGGCCT AAAACTATTG AACAGATTCA CAAAGAAGCT
    AAAATAGAAG AACAGGAAGA GCAGAGGAAG GTCCAACAAC TCATGACCAA AGAGAAGAGA
    AGACCAGGTG TCCAGAGAGT GGATGAAGGT GGGTGGAACA CTGTTCAAGG GGCCAAGAAT
    AGTCGGGTAT TGGACCCCTC AAAATTTCTG AAAATCACTA AGCCCACAAT TGATGAGAAA
    ATTCAGCTGG TACCTAAAGC GCAGCTGGGC AGCTGGGGAA AGGGCAGCAG TGGTGGAGCC
    AAGGCGAGCG AGGCCGATGC CTTAAGATCA AGTGCTTCCA GTTTAAACAG ATTCTCTGCT
    TTGCAACCTC CAGCACCTTC AGGGTCTGCG TCATCCACAC CTCTAGAGTT TGATTCCCGG
    CGGACCTTAA CTAGTCGAGG AAGCACGGGC AGGGAGAAGA ATGACAAGCC CCTCCAGCCT
    GCAGCAGCTC GGCCGAACAC TTTCATGAGG GGTGGCAGCA GTAAAGACCT GTTAGACAAT
    CAGTCTCAGG AGGAGCAGCG GAGGGAGATG CTGGAGACCG TGAAGCAGCT CACAGGAGGC
    ATGGACGTGG AGAGGAGCAG CGCTGAGGCG GAGCGAAGCA GGACCAGGGA GCCAGTGACA
    AAACCGGAAA TTCCAGTAAC ATCAGCCTCT GACAAGCCCG CGTTGTCAGA GGAAGAGATG
    GAGAGGAAGT CCAAATCTAT CATTGATGAG TTTCTACACA TTAATGATTT TAAGGAAGCC
    ATGCAGTGTG TGGAAGAGCT GAACGCCCAG GGCCTGCAAC ATGTGTTCGT GAGGACGGGA
    GTGGAGTCCA CCCTGGAAAG GAGCCAGATC ACCCGGGATC ACATGGGCCA GTTACTGTAT
    CAGCTGGTGC AGTCAGAAAA GCTCAGCAAA CAGGACTTTT TCAGAGGTTT TTCAGAAACA
    TTGGAATTGG CAGATGACAT GGCCATTGAT ATTCCCCATA TTTGGTTGTA CCTTGCTGAA
    CTGGTGACTC CCATGTTAAA AGAAGGCGGA ATCTCCATGA GACAACTCAT CACAGAATTT
    TGCAAACCTT TACTTCCTGT TGGAAGAGCT GGAATCTTGA TTTCTGAAAT ATTGCACCTT
    CTATGCAAAC AAATGAGCCA TAAGAAAGTG GGAGCCCTAT GGAGGGAGGC TGACCTCAGC
    TGGAAGGACT TTTTACCAGA AGGGGAAGAT GTACATAATT TTCTCTTGGA GCAGAAGTTG
    GACTTTTTAG AGTCTGACAG TTCCTGTTCC TCCGAAGCAC TTTTGAAGAA AGAACTCTCT
    GCCGAAGAGC TGTATAAGCG ACTCGAGAAA CTCATTATTG AGGACAAGGC AAATGATGAA
    CAGATCTTTG ACTGGGTAGA GGCTAATCTA GATGAAAGCC AGATGAGTTC ACCTACATTC
    CTTAGAGCTT TAATGACAGC CGTCTGTAAA GCAGCTATTA TAGCCGACTG TTCGACCTTC
    CGAGTGGACA CTGCTGTTAT CAAGCAGAGA GTGCCGATCT TACTCAAGTA CCTAGACTCA
    GATACAGAGA AGGAACTGCA AGCACTTTAT GCACTACAAG CATCAATAGT AAAACTTGAT
    CAACCTGCCA ATTTGCTACG GATGTTTTTT GATTGCCTGT ATGACGAGGA GGTGATCTCT
    GAGGATGCCT TCTACAAGTG GGAGAGCAGC AAGGACCCGG CAGAGCAGAA TGGGAAGGGT
    GTGGCCCTGA AGTCTGTCAC GGCGTTCTTC ACATGGCTGC GGGAAGCAGA AGAGGAGTCT
    GAGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317968.1
    CDS263..5134
    Translation

    Target ORF information:

    RefSeq Version XM_015462482.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462482.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTTTCCAGA GGCCTCAAAT ACAGCCTCCC 
    AGAGCCACCA TCCCGAACAG CAGCCCTTCC ATTCGACCCG GTGCACAGAC ACCCACTGCA
    GTGTATCAGG CCAATCAGCA CATCATGATG GTTAACCATC TGCCCATGCC GTACCCAGTG
    CCCCAGGGTC CTCAGTACTG TATACCACAG TACCGTCACA GTGGCCCTCC GTATGTTGGG
    CCACCACAAC AGTACCCGGT TCAACCACCA GGACCAGGTC CTTTTTATCC TGGGCCAGGA
    CCTGGGGACT TCCCCAGTGC TTACGGAACG CCTTTTTACC CAAGTCAGCC AGTGTACCAG
    TCAGCACCTA TCATAGTGCC TACGCAGCAG CAGCCCCCTC CAGCCAAGAG AGAGAAAAAA
    ACAATAAGAA TTCGGGATCC AAACCAGGGA GGTAAAGACA TAACAGAGGA GATTATGTCT
    GGAGGTGGCA GCCGAAATCC TACTCCACCC ATAGGGAGAC CCACTTCCAC ACCTACTCCT
    CCTCAGCAGC TGCCCAGCCA GGTCCCCGAG CACAGCCCTG TGGTCTATGG GACTGTGGAG
    AGCGCTCATC TTGCTGCCAG CACCCCTGTC ACTGCAGCGA GCGACCAGAA GCAAGAAGAG
    AAGCCAAAAC CAGATCCAGT GTTAAAGACT CCTTCCCCAG TCCTTAGGCT AGCCCTCAGT
    GGAGAGAAGA AAGAACAAGC AGGCCCGACG TCTGAGATTA CTGCAGTAGA GTCCATACCA
    GAGCTTCCTC TGCCTCCATC CCCTACTGCT ATTTCTCCAC TTGCTCGAAG TACAATTACT
    TCTCCCACCT CTGTTGCTCT TAGTAGCCAA CCAATATTCA CCACTGCAAT TGATGACAGA
    TGTGAACTCT CTTCCTCAAA AGAAGATACA ATTCCTATTC CCAGCCCCAC ATCTTGCACA
    GAAGCATCAG ATCCTTTACC AACAGATGAA ATTGATGATG ATATATGCAA GAAATCTTGT
    AGTGTAGCAC CAAATGATAT TCCACTGATT TCTAGTACTA ACCTAATTAA TGAAATGAAT
    GGAGTTAATG AAAAATTACC AGCCACGGAA AGCATTGTGG AAATAGTAAA ACAGGAGGTG
    TTACCGCTGA CTCTTGAATT GGAGATTTTG GAAAATCCCC CTGAAGAAAT CAAAGTGGAG
    TGTGTCCCAA CTCCCATCAC CCCTTCGATA GTTCCCTCCT CTTCTCCGTC TCCTCCAGCT
    CCTCCAGCTT CTCCTTCTCC CACACCAGTT ATCGTTCCTG CTGCTGCCAC CAGTGTTAAT
    ACTGGTCGTG GTTCCACCGG AGTCCAGAGA GCCTTCGAAG AGGGCGAGAA CATAAGAACT
    TGCCTTAGTG AAGATGCAAA AGAGATTCAG AACAAAGTAG AGGTAGAAGC AGATGGGCAA
    ACAGAAGAGA TTGTGGATTC TCAGAACTTA AGTTCAAGAA AGAGCCCAGT CCCAGCTCAG
    ACAGCTATAC CTGCACCAAA GATGTGGAAG AAACCAAAAG ACCGGACCCG AGCCACTGAA
    GAGTCGTTAG AGGCAGAATC AGAGCCTAAA GCTGAAGAGG AGCTTTCAGG TGACAAAATA
    CTTGAATCTG AACAGGATAA AATGAGCCAA GGGTTTCATC CTGAAAGAGA CCCCTCTGAC
    CTAAAAAACG TGAAAACTGT GGAAGAAAAT GGAGAAGAAG CTGAGCCTGT ACGTAATGGT
    GCTGAGAGTA TTTCTGAGGG TGAAGGAATA GATGCTAATT CAGGCTCCAC TGATAGTTCT
    GGTGAAGGGG TCACATTCCC ATTTAAGTCA GAATCCTGGA AGCCTCCTGA TTCGGAAGGC
    AAGAAGCAGT ATGACAGGGA GTTTTTGCTG GATTTCCAAT TCATGCCAGC GTGTATTCAG
    AAACCAGAGG GCCTGCCTCC CATCAGTGAT GTGGTTCTTG ACAAGATCAA TCAACCAAAA
    TTGCCAATGC GAACTCTGGA TCCTCGGATT TTGCCTCGAG GACCAGACTT TACTCCAGCC
    TTTGCAGATT TTGGAAGGCA AGCCCCTGGT GGAAGAGGTG TACCTGTTTG CAAAGTGCAG
    AGCAGGCATG GATTGCCAAT TCTGGAACAG AGCAAAGCCC CAGCTTCCAC TCCACTGGCG
    ATGTCGCACC CACCCCTGAA GAGCCTGCCT CTAGGGCTGT TGAATGTTGG AGCACGGAGA
    TCTCAGCCTG GCCAGAGGAG AGAGCCCAGG AAGATCATCA CGGTCTCCGT GAAAGAAGAC
    GTGCACCTGA AGAAGGCAGA GAACGCCTGG AAGCCCAGCC AGAGGCGGGA TAGCCCAGCC
    GAGGACCCCG AGAACATCAA GACCCAGGAA CTTTTTAGAA AAGTTCGAAG TATCTTAAAT
    AAATTGACAC CACAGATGTT CAACCAACTG ATGAAGCAAG TGTCAGGACT TACTGTTGAC
    ACAGAGGAGC GGCTGAAAGG AGTCATTGAC CTGGTCTTTG AGAAGGCTAT TGACGAACCC
    AGTTTCTCTG TGGCTTACGC AAACATGTGT CGATGTCTAG TAACGCTGAA AGTACCCATG
    GCAGACAAGC CTGGTAACAC AGTGAACTTC CGGAAGCTAC TACTGAACCG TTGCCAGAAG
    GAATTTGAAA AAGATAAAGC CGATGATGAT GTCTTTGAGA AGAAGCAGAA AGAACTCGAG
    GCTGCCAGTG CTCCAGAGGA GAGGACAAGG CTTCATGATG AACTGGAAGA AGCTAAAGAT
    AAAGCCCGGC GGAGATCCAT TGGCAACATC AAGTTCATTG GAGAACTCTT TAAACTCAAA
    ATGCTGACTG AAGCCATCAT GCATGACTGT GTGGTGAAGC TGCTGAAGAA CCACGATGAA
    GAATCCCTCG AGTGTCTGTG TCGCCTGCTC ACCACAATTG GCAAAGACCT GGACTTTGAG
    AAAGCAAAGC CACGTATGGA CCAGTATTTT AATCAGATGG AGAAAATTGT AAAAGAAAGA
    AAAACCTCAT CTAGGATTCG ATTCATGCTT CAGGACGTAA TAGATCTAAG GCTGTGTAAT
    TGGGTATCAC GACGAGCAGA TCAGGGGCCT AAAACTATTG AACAGATTCA CAAAGAAGCT
    AAAATAGAAG AACAGGAAGA GCAGAGGAAG GTCCAACAAC TCATGACCAA AGAGAAGAGA
    AGACCAGGTG TCCAGAGAGT GGATGAAGGT GGGTGGAACA CTGTTCAAGG GGCCAAGAAT
    AGTCGGGTAT TGGACCCCTC AAAATTTCTG AAAATCACTA AGCCCACAAT TGATGAGAAA
    ATTCAGCTGG TACCTAAAGC GCAGCTGGGC AGCTGGGGAA AGGGCAGCAG TGGTGGAGCC
    AAGGCGAGCG AGGCCGATGC CTTAAGATCA AGTGCTTCCA GTTTAAACAG ATTCTCTGCT
    TTGCAACCTC CAGCACCTTC AGGGTCTGCG TCATCCACAC CTCTAGAGTT TGATTCCCGG
    CGGACCTTAA CTAGTCGAGG AAGCACGGGC AGGGAGAAGA ATGACAAGCC CCTCCAGCCT
    GCAGCAGCTC GGCCGAACAC TTTCATGAGG GGTGGCAGCA GTAAAGACCT GTTAGACAAT
    CAGTCTCAGG AGGAGCAGCG GAGGGAGATG CTGGAGACCG TGAAGCAGCT CACAGGAGGC
    ATGGACGTGG AGAGGAGCAG CGCTGAGGCG GAGCGAAGCA GGACCAGGGA GCCAGTGACA
    AAACCGGAAA TTCCAGTAAC ATCAGCCTCT GACAAGCCCG CGTTGTCAGA GGAAGAGATG
    GAGAGGAAGT CCAAATCTAT CATTGATGAG TTTCTACACA TTAATGATTT TAAGGAAGCC
    ATGCAGTGTG TGGAAGAGCT GAACGCCCAG GGCCTGCAAC ATGTGTTCGT GAGGACGGGA
    GTGGAGTCCA CCCTGGAAAG GAGCCAGATC ACCCGGGATC ACATGGGCCA GTTACTGTAT
    CAGCTGGTGC AGTCAGAAAA GCTCAGCAAA CAGGACTTTT TCAGAGGTTT TTCAGAAACA
    TTGGAATTGG CAGATGACAT GGCCATTGAT ATTCCCCATA TTTGGTTGTA CCTTGCTGAA
    CTGGTGACTC CCATGTTAAA AGAAGGCGGA ATCTCCATGA GACAACTCAT CACAGAATTT
    TGCAAACCTT TACTTCCTGT TGGAAGAGCT GGAATCTTGA TTTCTGAAAT ATTGCACCTT
    CTATGCAAAC AAATGAGCCA TAAGAAAGTG GGAGCCCTAT GGAGGGAGGC TGACCTCAGC
    TGGAAGGACT TTTTACCAGA AGGGGAAGAT GTACATAATT TTCTCTTGGA GCAGAAGTTG
    GACTTTTTAG AGTCTGACAG TTCCTGTTCC TCCGAAGCAC TTTTGAAGAA AGAACTCTCT
    GCCGAAGAGC TGTATAAGCG ACTCGAGAAA CTCATTATTG AGGACAAGGC AAATGATGAA
    CAGATCTTTG ACTGGGTAGA GGCTAATCTA GATGAAAGCC AGATGAGTTC ACCTACATTC
    CTTAGAGCTT TAATGACAGC CGTCTGTAAA GCAGCTATTA TAGCCGACTG TTCGACCTTC
    CGAGTGGACA CTGCTGTTAT CAAGCAGAGA GTGCCGATCT TACTCAAGTA CCTAGACTCA
    GATACAGAGA AGGAACTGCA AGCACTTTAT GCACTACAAG CATCAATAGT AAAACTTGAT
    CAACCTGCCA ATTTGCTACG GATGTTTTTT GATTGCCTGT ATGACGAGGA GGTGATCTCT
    GAGGATGCCT TCTACAAGTG GGAGAGCAGC AAGGACCCGG CAGAGCAGAA TGGGAAGGGT
    GTGGCCCTGA AGTCTGTCAC GGCGTTCTTC ACATGGCTGC GGGAAGCAGA AGAGGAGTCT
    GAGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88096
    Clone ID Related Accession (Same CDS sequence) XM_015460886.2 , XM_024983008.1 , XM_015460886.1 , XM_015462482.1 , XM_024983012.1
    Accession Version XM_024983012.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTTTCCAGA GGCCTCAAAT ACAGCCTCCC 
    AGAGCCACCA TCCCGAACAG CAGCCCTTCC ATTCGACCCG GTGCACAGAC ACCCACTGCA
    GTGTATCAGG CCAATCAGCA CATCATGATG GTTAACCATC TGCCCATGCC GTACCCAGTG
    CCCCAGGGTC CTCAGTACTG TATACCACAG TACCGTCACA GTGGCCCTCC GTATGTTGGG
    CCACCACAAC AGTACCCGGT TCAACCACCA GGACCAGGTC CTTTTTATCC TGGGCCAGGA
    CCTGGGGACT TCCCCAGTGC TTACGGAACG CCTTTTTACC CAAGTCAGCC AGTGTACCAG
    TCAGCACCTA TCATAGTGCC TACGCAGCAG CAGCCCCCTC CAGCCAAGAG AGAGAAAAAA
    ACAATAAGAA TTCGGGATCC AAACCAGGGA GGTAAAGACA TAACAGAGGA GATTATGTCT
    GGAGGTGGCA GCCGAAATCC TACTCCACCC ATAGGGAGAC CCACTTCCAC ACCTACTCCT
    CCTCAGCAGC TGCCCAGCCA GGTCCCCGAG CACAGCCCTG TGGTCTATGG GACTGTGGAG
    AGCGCTCATC TTGCTGCCAG CACCCCTGTC ACTGCAGCGA GCGACCAGAA GCAAGAAGAG
    AAGCCAAAAC CAGATCCAGT GTTAAAGACT CCTTCCCCAG TCCTTAGGCT AGCCCTCAGT
    GGAGAGAAGA AAGAACAAGC AGGCCCGACG TCTGAGATTA CTGCAGTAGA GTCCATACCA
    GAGCTTCCTC TGCCTCCATC CCCTACTGCT ATTTCTCCAC TTGCTCGAAG TACAATTACT
    TCTCCCACCT CTGTTGCTCT TAGTAGCCAA CCAATATTCA CCACTGCAAT TGATGACAGA
    TGTGAACTCT CTTCCTCAAA AGAAGATACA ATTCCTATTC CCAGCCCCAC ATCTTGCACA
    GAAGCATCAG ATCCTTTACC AACAGATGAA ATTGATGATG ATATATGCAA GAAATCTTGT
    AGTGTAGCAC CAAATGATAT TCCACTGATT TCTAGTACTA ACCTAATTAA TGAAATGAAT
    GGAGTTAATG AAAAATTACC AGCCACGGAA AGCATTGTGG AAATAGTAAA ACAGGAGGTG
    TTACCGCTGA CTCTTGAATT GGAGATTTTG GAAAATCCCC CTGAAGAAAT CAAAGTGGAG
    TGTGTCCCAA CTCCCATCAC CCCTTCGATA GTTCCCTCCT CTTCTCCGTC TCCTCCAGCT
    CCTCCAGCTT CTCCTTCTCC CACACCAGTT ATCGTTCCTG CTGCTGCCAC CAGTGTTAAT
    ACTGGTCGTG GTTCCACCGG AGTCCAGAGA GCCTTCGAAG AGGGCGAGAA CATAAGAACT
    TGCCTTAGTG AAGATGCAAA AGAGATTCAG AACAAAGTAG AGGTAGAAGC AGATGGGCAA
    ACAGAAGAGA TTGTGGATTC TCAGAACTTA AGTTCAAGAA AGAGCCCAGT CCCAGCTCAG
    ACAGCTATAC CTGCACCAAA GATGTGGAAG AAACCAAAAG ACCGGACCCG AGCCACTGAA
    GAGTCGTTAG AGGCAGAATC AGAGCCTAAA GCTGAAGAGG AGCTTTCAGG TGACAAAATA
    CTTGAATCTG AACAGGATAA AATGAGCCAA GGGTTTCATC CTGAAAGAGA CCCCTCTGAC
    CTAAAAAACG TGAAAACTGT GGAAGAAAAT GGAGAAGAAG CTGAGCCTGT ACGTAATGGT
    GCTGAGAGTA TTTCTGAGGG TGAAGGAATA GATGCTAATT CAGGCTCCAC TGATAGTTCT
    GGTGAAGGGG TCACATTCCC ATTTAAGTCA GAATCCTGGA AGCCTCCTGA TTCGGAAGGC
    AAGAAGCAGT ATGACAGGGA GTTTTTGCTG GATTTCCAAT TCATGCCAGC GTGTATTCAG
    AAACCAGAGG GCCTGCCTCC CATCAGTGAT GTGGTTCTTG ACAAGATCAA TCAACCAAAA
    TTGCCAATGC GAACTCTGGA TCCTCGGATT TTGCCTCGAG GACCAGACTT TACTCCAGCC
    TTTGCAGATT TTGGAAGGCA AGCCCCTGGT GGAAGAGGTG TACCTGTTTG CAAAGTGCAG
    AGCAGGCATG GATTGCCAAT TCTGGAACAG AGCAAAGCCC CAGCTTCCAC TCCACTGGCG
    ATGTCGCACC CACCCCTGAA GAGCCTGCCT CTAGGGCTGT TGAATGTTGG AGCACGGAGA
    TCTCAGCCTG GCCAGAGGAG AGAGCCCAGG AAGATCATCA CGGTCTCCGT GAAAGAAGAC
    GTGCACCTGA AGAAGGCAGA GAACGCCTGG AAGCCCAGCC AGAGGCGGGA TAGCCCAGCC
    GAGGACCCCG AGAACATCAA GACCCAGGAA CTTTTTAGAA AAGTTCGAAG TATCTTAAAT
    AAATTGACAC CACAGATGTT CAACCAACTG ATGAAGCAAG TGTCAGGACT TACTGTTGAC
    ACAGAGGAGC GGCTGAAAGG AGTCATTGAC CTGGTCTTTG AGAAGGCTAT TGACGAACCC
    AGTTTCTCTG TGGCTTACGC AAACATGTGT CGATGTCTAG TAACGCTGAA AGTACCCATG
    GCAGACAAGC CTGGTAACAC AGTGAACTTC CGGAAGCTAC TACTGAACCG TTGCCAGAAG
    GAATTTGAAA AAGATAAAGC CGATGATGAT GTCTTTGAGA AGAAGCAGAA AGAACTCGAG
    GCTGCCAGTG CTCCAGAGGA GAGGACAAGG CTTCATGATG AACTGGAAGA AGCTAAAGAT
    AAAGCCCGGC GGAGATCCAT TGGCAACATC AAGTTCATTG GAGAACTCTT TAAACTCAAA
    ATGCTGACTG AAGCCATCAT GCATGACTGT GTGGTGAAGC TGCTGAAGAA CCACGATGAA
    GAATCCCTCG AGTGTCTGTG TCGCCTGCTC ACCACAATTG GCAAAGACCT GGACTTTGAG
    AAAGCAAAGC CACGTATGGA CCAGTATTTT AATCAGATGG AGAAAATTGT AAAAGAAAGA
    AAAACCTCAT CTAGGATTCG ATTCATGCTT CAGGACGTAA TAGATCTAAG GCTGTGTAAT
    TGGGTATCAC GACGAGCAGA TCAGGGGCCT AAAACTATTG AACAGATTCA CAAAGAAGCT
    AAAATAGAAG AACAGGAAGA GCAGAGGAAG GTCCAACAAC TCATGACCAA AGAGAAGAGA
    AGACCAGGTG TCCAGAGAGT GGATGAAGGT GGGTGGAACA CTGTTCAAGG GGCCAAGAAT
    AGTCGGGTAT TGGACCCCTC AAAATTTCTG AAAATCACTA AGCCCACAAT TGATGAGAAA
    ATTCAGCTGG TACCTAAAGC GCAGCTGGGC AGCTGGGGAA AGGGCAGCAG TGGTGGAGCC
    AAGGCGAGCG AGGCCGATGC CTTAAGATCA AGTGCTTCCA GTTTAAACAG ATTCTCTGCT
    TTGCAACCTC CAGCACCTTC AGGGTCTGCG TCATCCACAC CTCTAGAGTT TGATTCCCGG
    CGGACCTTAA CTAGTCGAGG AAGCACGGGC AGGGAGAAGA ATGACAAGCC CCTCCAGCCT
    GCAGCAGCTC GGCCGAACAC TTTCATGAGG GGTGGCAGCA GTAAAGACCT GTTAGACAAT
    CAGTCTCAGG AGGAGCAGCG GAGGGAGATG CTGGAGACCG TGAAGCAGCT CACAGGAGGC
    ATGGACGTGG AGAGGAGCAG CGCTGAGGCG GAGCGAAGCA GGACCAGGGA GCCAGTGACA
    AAACCGGAAA TTCCAGTAAC ATCAGCCTCT GACAAGCCCG CGTTGTCAGA GGAAGAGATG
    GAGAGGAAGT CCAAATCTAT CATTGATGAG TTTCTACACA TTAATGATTT TAAGGAAGCC
    ATGCAGTGTG TGGAAGAGCT GAACGCCCAG GGCCTGCAAC ATGTGTTCGT GAGGACGGGA
    GTGGAGTCCA CCCTGGAAAG GAGCCAGATC ACCCGGGATC ACATGGGCCA GTTACTGTAT
    CAGCTGGTGC AGTCAGAAAA GCTCAGCAAA CAGGACTTTT TCAGAGGTTT TTCAGAAACA
    TTGGAATTGG CAGATGACAT GGCCATTGAT ATTCCCCATA TTTGGTTGTA CCTTGCTGAA
    CTGGTGACTC CCATGTTAAA AGAAGGCGGA ATCTCCATGA GACAACTCAT CACAGAATTT
    TGCAAACCTT TACTTCCTGT TGGAAGAGCT GGAATCTTGA TTTCTGAAAT ATTGCACCTT
    CTATGCAAAC AAATGAGCCA TAAGAAAGTG GGAGCCCTAT GGAGGGAGGC TGACCTCAGC
    TGGAAGGACT TTTTACCAGA AGGGGAAGAT GTACATAATT TTCTCTTGGA GCAGAAGTTG
    GACTTTTTAG AGTCTGACAG TTCCTGTTCC TCCGAAGCAC TTTTGAAGAA AGAACTCTCT
    GCCGAAGAGC TGTATAAGCG ACTCGAGAAA CTCATTATTG AGGACAAGGC AAATGATGAA
    CAGATCTTTG ACTGGGTAGA GGCTAATCTA GATGAAAGCC AGATGAGTTC ACCTACATTC
    CTTAGAGCTT TAATGACAGC CGTCTGTAAA GCAGCTATTA TAGCCGACTG TTCGACCTTC
    CGAGTGGACA CTGCTGTTAT CAAGCAGAGA GTGCCGATCT TACTCAAGTA CCTAGACTCA
    GATACAGAGA AGGAACTGCA AGCACTTTAT GCACTACAAG CATCAATAGT AAAACTTGAT
    CAACCTGCCA ATTTGCTACG GATGTTTTTT GATTGCCTGT ATGACGAGGA GGTGATCTCT
    GAGGATGCCT TCTACAAGTG GGAGAGCAGC AAGGACCCGG CAGAGCAGAA TGGGAAGGGT
    GTGGCCCTGA AGTCTGTCAC GGCGTTCTTC ACATGGCTGC GGGAAGCAGA AGAGGAGTCT
    GAGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838780.1
    CDS133..5004
    Translation

    Target ORF information:

    RefSeq Version XM_024983012.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983012.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTTTCCAGA GGCCTCAAAT ACAGCCTCCC 
    AGAGCCACCA TCCCGAACAG CAGCCCTTCC ATTCGACCCG GTGCACAGAC ACCCACTGCA
    GTGTATCAGG CCAATCAGCA CATCATGATG GTTAACCATC TGCCCATGCC GTACCCAGTG
    CCCCAGGGTC CTCAGTACTG TATACCACAG TACCGTCACA GTGGCCCTCC GTATGTTGGG
    CCACCACAAC AGTACCCGGT TCAACCACCA GGACCAGGTC CTTTTTATCC TGGGCCAGGA
    CCTGGGGACT TCCCCAGTGC TTACGGAACG CCTTTTTACC CAAGTCAGCC AGTGTACCAG
    TCAGCACCTA TCATAGTGCC TACGCAGCAG CAGCCCCCTC CAGCCAAGAG AGAGAAAAAA
    ACAATAAGAA TTCGGGATCC AAACCAGGGA GGTAAAGACA TAACAGAGGA GATTATGTCT
    GGAGGTGGCA GCCGAAATCC TACTCCACCC ATAGGGAGAC CCACTTCCAC ACCTACTCCT
    CCTCAGCAGC TGCCCAGCCA GGTCCCCGAG CACAGCCCTG TGGTCTATGG GACTGTGGAG
    AGCGCTCATC TTGCTGCCAG CACCCCTGTC ACTGCAGCGA GCGACCAGAA GCAAGAAGAG
    AAGCCAAAAC CAGATCCAGT GTTAAAGACT CCTTCCCCAG TCCTTAGGCT AGCCCTCAGT
    GGAGAGAAGA AAGAACAAGC AGGCCCGACG TCTGAGATTA CTGCAGTAGA GTCCATACCA
    GAGCTTCCTC TGCCTCCATC CCCTACTGCT ATTTCTCCAC TTGCTCGAAG TACAATTACT
    TCTCCCACCT CTGTTGCTCT TAGTAGCCAA CCAATATTCA CCACTGCAAT TGATGACAGA
    TGTGAACTCT CTTCCTCAAA AGAAGATACA ATTCCTATTC CCAGCCCCAC ATCTTGCACA
    GAAGCATCAG ATCCTTTACC AACAGATGAA ATTGATGATG ATATATGCAA GAAATCTTGT
    AGTGTAGCAC CAAATGATAT TCCACTGATT TCTAGTACTA ACCTAATTAA TGAAATGAAT
    GGAGTTAATG AAAAATTACC AGCCACGGAA AGCATTGTGG AAATAGTAAA ACAGGAGGTG
    TTACCGCTGA CTCTTGAATT GGAGATTTTG GAAAATCCCC CTGAAGAAAT CAAAGTGGAG
    TGTGTCCCAA CTCCCATCAC CCCTTCGATA GTTCCCTCCT CTTCTCCGTC TCCTCCAGCT
    CCTCCAGCTT CTCCTTCTCC CACACCAGTT ATCGTTCCTG CTGCTGCCAC CAGTGTTAAT
    ACTGGTCGTG GTTCCACCGG AGTCCAGAGA GCCTTCGAAG AGGGCGAGAA CATAAGAACT
    TGCCTTAGTG AAGATGCAAA AGAGATTCAG AACAAAGTAG AGGTAGAAGC AGATGGGCAA
    ACAGAAGAGA TTGTGGATTC TCAGAACTTA AGTTCAAGAA AGAGCCCAGT CCCAGCTCAG
    ACAGCTATAC CTGCACCAAA GATGTGGAAG AAACCAAAAG ACCGGACCCG AGCCACTGAA
    GAGTCGTTAG AGGCAGAATC AGAGCCTAAA GCTGAAGAGG AGCTTTCAGG TGACAAAATA
    CTTGAATCTG AACAGGATAA AATGAGCCAA GGGTTTCATC CTGAAAGAGA CCCCTCTGAC
    CTAAAAAACG TGAAAACTGT GGAAGAAAAT GGAGAAGAAG CTGAGCCTGT ACGTAATGGT
    GCTGAGAGTA TTTCTGAGGG TGAAGGAATA GATGCTAATT CAGGCTCCAC TGATAGTTCT
    GGTGAAGGGG TCACATTCCC ATTTAAGTCA GAATCCTGGA AGCCTCCTGA TTCGGAAGGC
    AAGAAGCAGT ATGACAGGGA GTTTTTGCTG GATTTCCAAT TCATGCCAGC GTGTATTCAG
    AAACCAGAGG GCCTGCCTCC CATCAGTGAT GTGGTTCTTG ACAAGATCAA TCAACCAAAA
    TTGCCAATGC GAACTCTGGA TCCTCGGATT TTGCCTCGAG GACCAGACTT TACTCCAGCC
    TTTGCAGATT TTGGAAGGCA AGCCCCTGGT GGAAGAGGTG TACCTGTTTG CAAAGTGCAG
    AGCAGGCATG GATTGCCAAT TCTGGAACAG AGCAAAGCCC CAGCTTCCAC TCCACTGGCG
    ATGTCGCACC CACCCCTGAA GAGCCTGCCT CTAGGGCTGT TGAATGTTGG AGCACGGAGA
    TCTCAGCCTG GCCAGAGGAG AGAGCCCAGG AAGATCATCA CGGTCTCCGT GAAAGAAGAC
    GTGCACCTGA AGAAGGCAGA GAACGCCTGG AAGCCCAGCC AGAGGCGGGA TAGCCCAGCC
    GAGGACCCCG AGAACATCAA GACCCAGGAA CTTTTTAGAA AAGTTCGAAG TATCTTAAAT
    AAATTGACAC CACAGATGTT CAACCAACTG ATGAAGCAAG TGTCAGGACT TACTGTTGAC
    ACAGAGGAGC GGCTGAAAGG AGTCATTGAC CTGGTCTTTG AGAAGGCTAT TGACGAACCC
    AGTTTCTCTG TGGCTTACGC AAACATGTGT CGATGTCTAG TAACGCTGAA AGTACCCATG
    GCAGACAAGC CTGGTAACAC AGTGAACTTC CGGAAGCTAC TACTGAACCG TTGCCAGAAG
    GAATTTGAAA AAGATAAAGC CGATGATGAT GTCTTTGAGA AGAAGCAGAA AGAACTCGAG
    GCTGCCAGTG CTCCAGAGGA GAGGACAAGG CTTCATGATG AACTGGAAGA AGCTAAAGAT
    AAAGCCCGGC GGAGATCCAT TGGCAACATC AAGTTCATTG GAGAACTCTT TAAACTCAAA
    ATGCTGACTG AAGCCATCAT GCATGACTGT GTGGTGAAGC TGCTGAAGAA CCACGATGAA
    GAATCCCTCG AGTGTCTGTG TCGCCTGCTC ACCACAATTG GCAAAGACCT GGACTTTGAG
    AAAGCAAAGC CACGTATGGA CCAGTATTTT AATCAGATGG AGAAAATTGT AAAAGAAAGA
    AAAACCTCAT CTAGGATTCG ATTCATGCTT CAGGACGTAA TAGATCTAAG GCTGTGTAAT
    TGGGTATCAC GACGAGCAGA TCAGGGGCCT AAAACTATTG AACAGATTCA CAAAGAAGCT
    AAAATAGAAG AACAGGAAGA GCAGAGGAAG GTCCAACAAC TCATGACCAA AGAGAAGAGA
    AGACCAGGTG TCCAGAGAGT GGATGAAGGT GGGTGGAACA CTGTTCAAGG GGCCAAGAAT
    AGTCGGGTAT TGGACCCCTC AAAATTTCTG AAAATCACTA AGCCCACAAT TGATGAGAAA
    ATTCAGCTGG TACCTAAAGC GCAGCTGGGC AGCTGGGGAA AGGGCAGCAG TGGTGGAGCC
    AAGGCGAGCG AGGCCGATGC CTTAAGATCA AGTGCTTCCA GTTTAAACAG ATTCTCTGCT
    TTGCAACCTC CAGCACCTTC AGGGTCTGCG TCATCCACAC CTCTAGAGTT TGATTCCCGG
    CGGACCTTAA CTAGTCGAGG AAGCACGGGC AGGGAGAAGA ATGACAAGCC CCTCCAGCCT
    GCAGCAGCTC GGCCGAACAC TTTCATGAGG GGTGGCAGCA GTAAAGACCT GTTAGACAAT
    CAGTCTCAGG AGGAGCAGCG GAGGGAGATG CTGGAGACCG TGAAGCAGCT CACAGGAGGC
    ATGGACGTGG AGAGGAGCAG CGCTGAGGCG GAGCGAAGCA GGACCAGGGA GCCAGTGACA
    AAACCGGAAA TTCCAGTAAC ATCAGCCTCT GACAAGCCCG CGTTGTCAGA GGAAGAGATG
    GAGAGGAAGT CCAAATCTAT CATTGATGAG TTTCTACACA TTAATGATTT TAAGGAAGCC
    ATGCAGTGTG TGGAAGAGCT GAACGCCCAG GGCCTGCAAC ATGTGTTCGT GAGGACGGGA
    GTGGAGTCCA CCCTGGAAAG GAGCCAGATC ACCCGGGATC ACATGGGCCA GTTACTGTAT
    CAGCTGGTGC AGTCAGAAAA GCTCAGCAAA CAGGACTTTT TCAGAGGTTT TTCAGAAACA
    TTGGAATTGG CAGATGACAT GGCCATTGAT ATTCCCCATA TTTGGTTGTA CCTTGCTGAA
    CTGGTGACTC CCATGTTAAA AGAAGGCGGA ATCTCCATGA GACAACTCAT CACAGAATTT
    TGCAAACCTT TACTTCCTGT TGGAAGAGCT GGAATCTTGA TTTCTGAAAT ATTGCACCTT
    CTATGCAAAC AAATGAGCCA TAAGAAAGTG GGAGCCCTAT GGAGGGAGGC TGACCTCAGC
    TGGAAGGACT TTTTACCAGA AGGGGAAGAT GTACATAATT TTCTCTTGGA GCAGAAGTTG
    GACTTTTTAG AGTCTGACAG TTCCTGTTCC TCCGAAGCAC TTTTGAAGAA AGAACTCTCT
    GCCGAAGAGC TGTATAAGCG ACTCGAGAAA CTCATTATTG AGGACAAGGC AAATGATGAA
    CAGATCTTTG ACTGGGTAGA GGCTAATCTA GATGAAAGCC AGATGAGTTC ACCTACATTC
    CTTAGAGCTT TAATGACAGC CGTCTGTAAA GCAGCTATTA TAGCCGACTG TTCGACCTTC
    CGAGTGGACA CTGCTGTTAT CAAGCAGAGA GTGCCGATCT TACTCAAGTA CCTAGACTCA
    GATACAGAGA AGGAACTGCA AGCACTTTAT GCACTACAAG CATCAATAGT AAAACTTGAT
    CAACCTGCCA ATTTGCTACG GATGTTTTTT GATTGCCTGT ATGACGAGGA GGTGATCTCT
    GAGGATGCCT TCTACAAGTG GGAGAGCAGC AAGGACCCGG CAGAGCAGAA TGGGAAGGGT
    GTGGCCCTGA AGTCTGTCAC GGCGTTCTTC ACATGGCTGC GGGAAGCAGA AGAGGAGTCT
    GAGGATAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03120
    Clone ID Related Accession (Same CDS sequence) XM_015460883.2 , XM_015462479.1 , XM_024982991.1 , XM_015460883.1
    Accession Version XM_015460883.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460883.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGTTTTTCC AGAGGCCTCA AATACAGCCT CCCAGAGCCA CCATCCCGAA CAGCAGCCCT
    TCCATTCGAC CCGGTGCACA GACACCCACT GCAGTGTATC AGGCCAATCA GCACATCATG
    ATGGTTAACC ATCTGCCCAT GCCGTACCCA GTGCCCCAGG GTCCTCAGTA CTGTATACCA
    CAGTACCGTC ACAGTGGCCC TCCGTATGTT GGGCCACCAC AACAGTACCC GGTTCAACCA
    CCAGGACCAG GTCCTTTTTA TCCTGGGCCA GGACCTGGGG ACTTCCCCAG TGCTTACGGA
    ACGCCTTTTT ACCCAAGTCA GCCAGTGTAC CAGTCAGCAC CTATCATAGT GCCTACGCAG
    CAGCAGCCCC CTCCAGCCAA GAGAGAGAAA AAAACAATAA GAATTCGGGA TCCAAACCAG
    GGAGGTAAAG ACATAACAGA GGAGATTATG TCTGGAGGTG GCAGCCGAAA TCCTACTCCA
    CCCATAGGGA GACCCACTTC CACACCTACT CCTCCTCAGC AGCTGCCCAG CCAGGTCCCC
    GAGCACAGCC CTGTGGTCTA TGGGACTGTG GAGAGCGCTC ATCTTGCTGC CAGCACCCCT
    GTCACTGCAG CGAGCGACCA GAAGCAAGAA GAGAAGCCAA AACCAGATCC AGTGTTAAAG
    ACTCCTTCCC CAGTCCTTAG GCTAGCCCTC AGTGGAGAGA AGAAAGAACA AGCAGGCCCG
    ACGTCTGAGA TTACTGCAGT AGAGTCCATA CCAGAGCTTC CTCTGCCTCC ATCCCCTACT
    GCTATTTCTC CACTTGCTCG AAGTACAATT ACTTCTCCCA CCTCTGTTGC TCTTAGTAGC
    CAACCAATAT TCACCACTGC AATTGATGAC AGATGTGAAC TCTCTTCCTC AAAAGAAGAT
    ACAATTCCTA TTCCCAGCCC CACATCTTGC ACAGAAGCAT CAGATCCTTT ACCAACAGAT
    GAAATTGATG ATGATATATG CAAGAAATCT TGTAGTGTAG CACCAAATGA TATTCCACTG
    ATTTCTAGTA CTAACCTAAT TAATGAAATG AATGGAGTTA ATGAAAAATT ACCAGCCACG
    GAAAGCATTG TGGAAATAGT AAAACAGGAG GTGTTACCGC TGACTCTTGA ATTGGAGATT
    TTGGAAAATC CCCCTGAAGA AATCAAAGTG GAGTGTGTCC CAACTCCCAT CACCCCTTCG
    ATAGTTCCCT CCTCTTCTCC GTCTCCTCCA GCTCCTCCAG CTTCTCCTTC TCCCACACCA
    GTTATCGTTC CTGCTGCTGC CACCAGTGTT AATACTGGTC GTGGTTCCAC CGGAGTCCAG
    AGAGCCTTCG AAGAGGGCGA GAACATAAGA ACTTGCCTTA GTGAAGATGC AAAAGAGATT
    CAGAACAAAG TAGAGGTAGA AGCAGATGGG CAAACAGAAG AGATTGTGGA TTCTCAGAAC
    TTAAGTTCAA GAAAGAGCCC AGTCCCAGCT CAGACAGCTA TACCTGCACC AAAGATGTGG
    AAGAAACCAA AAGACCGGAC CCGAGCCACT GAAGAGTCGT TAGAGGCAGA ATCAGAGCCT
    AAAGCTGAAG AGGAGCTTTC AGGTGACAAA ATACTTGAAT CTGAACAGGA TAAAATGAGC
    CAAGGGTTTC ATCCTGAAAG AGACCCCTCT GACCTAAAAA ACGTGAAAAC TGTGGAAGAA
    AATGGAGAAG AAGCTGAGCC TGTACGTAAT GGTGCTGAGA GTATTTCTGA GGGTGAAGGA
    ATAGATGCTA ATTCAGGCTC CACTGATAGT TCTGGTGAAG GGGTCACATT CCCATTTAAG
    TCAGAATCCT GGAAGCCTCC TGATTCGGAA GGCAAGAAGC AGTATGACAG GGAGTTTTTG
    CTGGATTTCC AATTCATGCC AGCGTGTATT CAGAAACCAG AGGGCCTGCC TCCCATCAGT
    GATGTGGTTC TTGACAAGAT CAATCAACCA AAATTGCCAA TGCGAACTCT GGATCCTCGG
    ATTTTGCCTC GAGGACCAGA CTTTACTCCA GCCTTTGCAG ATTTTGGAAG GCAAGCCCCT
    GGTGGAAGAG GTGTACCTGT TTGCAAAGTG CAGAGCAGGC ATGGATTGCC AATTCTGGAA
    CAGAGCAAAG CCCCAGCTTC CACTCCACTG GCGATGTCGC ACCCACCCCT GAAGAGCCTG
    CCTCTAGGGC TGTTGAATGT TGGAGCACGG AGATCTCAGC CTGGCCAGAG GAGAGAGCCC
    AGGAAGATCA TCACGGTCTC CGTGAAAGAA GACGTGCACC TGAAGAAGGC AGAGAACGCC
    TGGAAGCCCA GCCAGAGGCG GGATAGCCCA GCCGAGGACC CCGAGAACAT CAAGACCCAG
    GAACTTTTTA GAAAAGTTCG AAGTATCTTA AATAAATTGA CACCACAGAT GTTCAACCAA
    CTGATGAAGC AAGTGTCAGG ACTTACTGTT GACACAGAGG AGCGGCTGAA AGGAGTCATT
    GACCTGGTCT TTGAGAAGGC TATTGACGAA CCCAGTTTCT CTGTGGCTTA CGCAAACATG
    TGTCGATGTC TAGTAACGCT GAAAGTACCC ATGGCAGACA AGCCTGGTAA CACAGTGAAC
    TTCCGGAAGC TACTACTGAA CCGTTGCCAG AAGGAATTTG AAAAAGATAA AGCCGATGAT
    GATGTCTTTG AGAAGAAGCA GAAAGAACTC GAGGCTGCCA GTGCTCCAGA GGAGAGGACA
    AGGCTTCATG ATGAACTGGA AGAAGCTAAA GATAAAGCCC GGCGGAGATC CATTGGCAAC
    ATCAAGTTCA TTGGAGAACT CTTTAAACTC AAAATGCTGA CTGAAGCCAT CATGCATGAC
    TGTGTGGTGA AGCTGCTGAA GAACCACGAT GAAGAATCCC TCGAGTGTCT GTGTCGCCTG
    CTCACCACAA TTGGCAAAGA CCTGGACTTT GAGAAAGCAA AGCCACGTAT GGACCAGTAT
    TTTAATCAGA TGGAGAAAAT TGTAAAAGAA AGAAAAACCT CATCTAGGAT TCGATTCATG
    CTTCAGGACG TAATAGATCT AAGGCTGTGT AATTGGGTAT CACGACGAGC AGATCAGGGG
    CCTAAAACTA TTGAACAGAT TCACAAAGAA GCTAAAATAG AAGAACAGGA AGAGCAGAGG
    AAGGTCCAAC AACTCATGAC CAAAGAGAAG AGAAGACCAG GTGTCCAGAG AGTGGATGAA
    GGTGGGTGGA ACACTGTTCA AGGGGCCAAG AATAGTCGGG TATTGGACCC CTCAAAATTT
    CTGAAAATCA CTAAGCCCAC AATTGATGAG AAAATTCAGC TGGTACCTAA AGCGCAGCTG
    GGCAGCTGGG GAAAGGGCAG CAGTGGTGGA GCCAAGGCGA GCGAGGCCGA TGCCTTAAGA
    TCAAGTGCTT CCAGTTTAAA CAGATTCTCT GCTTTGCAAC CTCCAGCACC TTCAGGGTCT
    GCGTCATCCA CACCTCTAGA GTTTGATTCC CGGCGGACCT TAACTAGTCG AGGAAGCACG
    GGCAGGGAGA AGAATGACAA GCCCCTCCAG CCTGCAGCAG CTCGGCCGAA CACTTTCATG
    AGGGGTGGCA GCAGTAAAGA CCTGTTAGAC AATCAGTCTC AGGAGGAGCA GCGGAGGGAG
    ATGCTGGAGA CCGTGAAGCA GCTCACAGGA GGCATGGACG TGGAGAGGAG CAGCGCTGAG
    GCGGAGCGAA GCAGGACCAG GGAGCCAGTG ACAAAACCGG AAATTCCAGT AACATCAGCC
    TCTGACAAGC CCGCGTTGTC AGAGGAAGAG ATGGAGAGGA AGTCCAAATC TATCATTGAT
    GAGTTTCTAC ACATTAATGA TTTTAAGGAA GCCATGCAGT GTGTGGAAGA GCTGAACGCC
    CAGGGCCTGC AACATGTGTT CGTGAGGACG GGAGTGGAGT CCACCCTGGA AAGGAGCCAG
    ATCACCCGGG ATCACATGGG CCAGTTACTG TATCAGCTGG TGCAGTCAGA AAAGCTCAGC
    AAACAGGACT TTTTCAGAGG TTTTTCAGAA ACATTGGAAT TGGCAGATGA CATGGCCATT
    GATATTCCCC ATATTTGGTT GTACCTTGCT GAACTGGTGA CTCCCATGTT AAAAGAAGGC
    GGAATCTCCA TGAGACAACT CATCACAGAA TTTTGCAAAC CTTTACTTCC TGTTGGAAGA
    GCTGGAATCT TGATTTCTGA AATATTGCAC CTTCTATGCA AACAAATGAG CCATAAGAAA
    GTGGGAGCCC TATGGAGGGA GGCTGACCTC AGCTGGAAGG ACTTTTTACC AGAAGGGGAA
    GATGTACATA ATTTTCTCTT GGAGCAGAAG TTGGACTTTT TAGAGTCTGA CAGTTCCTGT
    TCCTCCGAAG CACTTTTGAA GAAAGAACTC TCTGCCGAAG AGCTGTATAA GCGACTCGAG
    AAACTCATTA TTGAGGACAA GGCAAATGAT GAACAGATCT TTGACTGGGT AGAGGCTAAT
    CTAGATGAAA GCCAGATGAG TTCACCTACA TTCCTTAGAG CTTTAATGAC AGCCGTCTGT
    AAAGCAGCTA TTATAGCCGA CTGTTCGACC TTCCGAGTGG ACACTGCTGT TATCAAGCAG
    AGAGTGCCGA TCTTACTCAA GTACCTAGAC TCAGATACAG AGAAGGAACT GCAAGCACTT
    TATGCACTAC AAGCATCAAT AGTAAAACTT GATCAACCTG CCAATTTGCT ACGGATGTTT
    TTTGATTGCC TGTATGACGA GGAGGTGATC TCTGAGGATG CCTTCTACAA GTGGGAGAGC
    AGCAAGGACC CGGCAGAGCA GAATGGGAAG GGTGTGGCCC TGAAGTCTGT CACGGCGTTC
    TTCACATGGC TGCGGGAAGC AGAAGAGGAG TCTGAGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316369.1
    CDS196..5100
    Translation

    Target ORF information:

    RefSeq Version XM_015460883.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460883.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGTTTTTCC AGAGGCCTCA AATACAGCCT CCCAGAGCCA CCATCCCGAA CAGCAGCCCT
    TCCATTCGAC CCGGTGCACA GACACCCACT GCAGTGTATC AGGCCAATCA GCACATCATG
    ATGGTTAACC ATCTGCCCAT GCCGTACCCA GTGCCCCAGG GTCCTCAGTA CTGTATACCA
    CAGTACCGTC ACAGTGGCCC TCCGTATGTT GGGCCACCAC AACAGTACCC GGTTCAACCA
    CCAGGACCAG GTCCTTTTTA TCCTGGGCCA GGACCTGGGG ACTTCCCCAG TGCTTACGGA
    ACGCCTTTTT ACCCAAGTCA GCCAGTGTAC CAGTCAGCAC CTATCATAGT GCCTACGCAG
    CAGCAGCCCC CTCCAGCCAA GAGAGAGAAA AAAACAATAA GAATTCGGGA TCCAAACCAG
    GGAGGTAAAG ACATAACAGA GGAGATTATG TCTGGAGGTG GCAGCCGAAA TCCTACTCCA
    CCCATAGGGA GACCCACTTC CACACCTACT CCTCCTCAGC AGCTGCCCAG CCAGGTCCCC
    GAGCACAGCC CTGTGGTCTA TGGGACTGTG GAGAGCGCTC ATCTTGCTGC CAGCACCCCT
    GTCACTGCAG CGAGCGACCA GAAGCAAGAA GAGAAGCCAA AACCAGATCC AGTGTTAAAG
    ACTCCTTCCC CAGTCCTTAG GCTAGCCCTC AGTGGAGAGA AGAAAGAACA AGCAGGCCCG
    ACGTCTGAGA TTACTGCAGT AGAGTCCATA CCAGAGCTTC CTCTGCCTCC ATCCCCTACT
    GCTATTTCTC CACTTGCTCG AAGTACAATT ACTTCTCCCA CCTCTGTTGC TCTTAGTAGC
    CAACCAATAT TCACCACTGC AATTGATGAC AGATGTGAAC TCTCTTCCTC AAAAGAAGAT
    ACAATTCCTA TTCCCAGCCC CACATCTTGC ACAGAAGCAT CAGATCCTTT ACCAACAGAT
    GAAATTGATG ATGATATATG CAAGAAATCT TGTAGTGTAG CACCAAATGA TATTCCACTG
    ATTTCTAGTA CTAACCTAAT TAATGAAATG AATGGAGTTA ATGAAAAATT ACCAGCCACG
    GAAAGCATTG TGGAAATAGT AAAACAGGAG GTGTTACCGC TGACTCTTGA ATTGGAGATT
    TTGGAAAATC CCCCTGAAGA AATCAAAGTG GAGTGTGTCC CAACTCCCAT CACCCCTTCG
    ATAGTTCCCT CCTCTTCTCC GTCTCCTCCA GCTCCTCCAG CTTCTCCTTC TCCCACACCA
    GTTATCGTTC CTGCTGCTGC CACCAGTGTT AATACTGGTC GTGGTTCCAC CGGAGTCCAG
    AGAGCCTTCG AAGAGGGCGA GAACATAAGA ACTTGCCTTA GTGAAGATGC AAAAGAGATT
    CAGAACAAAG TAGAGGTAGA AGCAGATGGG CAAACAGAAG AGATTGTGGA TTCTCAGAAC
    TTAAGTTCAA GAAAGAGCCC AGTCCCAGCT CAGACAGCTA TACCTGCACC AAAGATGTGG
    AAGAAACCAA AAGACCGGAC CCGAGCCACT GAAGAGTCGT TAGAGGCAGA ATCAGAGCCT
    AAAGCTGAAG AGGAGCTTTC AGGTGACAAA ATACTTGAAT CTGAACAGGA TAAAATGAGC
    CAAGGGTTTC ATCCTGAAAG AGACCCCTCT GACCTAAAAA ACGTGAAAAC TGTGGAAGAA
    AATGGAGAAG AAGCTGAGCC TGTACGTAAT GGTGCTGAGA GTATTTCTGA GGGTGAAGGA
    ATAGATGCTA ATTCAGGCTC CACTGATAGT TCTGGTGAAG GGGTCACATT CCCATTTAAG
    TCAGAATCCT GGAAGCCTCC TGATTCGGAA GGCAAGAAGC AGTATGACAG GGAGTTTTTG
    CTGGATTTCC AATTCATGCC AGCGTGTATT CAGAAACCAG AGGGCCTGCC TCCCATCAGT
    GATGTGGTTC TTGACAAGAT CAATCAACCA AAATTGCCAA TGCGAACTCT GGATCCTCGG
    ATTTTGCCTC GAGGACCAGA CTTTACTCCA GCCTTTGCAG ATTTTGGAAG GCAAGCCCCT
    GGTGGAAGAG GTGTACCTGT TTGCAAAGTG CAGAGCAGGC ATGGATTGCC AATTCTGGAA
    CAGAGCAAAG CCCCAGCTTC CACTCCACTG GCGATGTCGC ACCCACCCCT GAAGAGCCTG
    CCTCTAGGGC TGTTGAATGT TGGAGCACGG AGATCTCAGC CTGGCCAGAG GAGAGAGCCC
    AGGAAGATCA TCACGGTCTC CGTGAAAGAA GACGTGCACC TGAAGAAGGC AGAGAACGCC
    TGGAAGCCCA GCCAGAGGCG GGATAGCCCA GCCGAGGACC CCGAGAACAT CAAGACCCAG
    GAACTTTTTA GAAAAGTTCG AAGTATCTTA AATAAATTGA CACCACAGAT GTTCAACCAA
    CTGATGAAGC AAGTGTCAGG ACTTACTGTT GACACAGAGG AGCGGCTGAA AGGAGTCATT
    GACCTGGTCT TTGAGAAGGC TATTGACGAA CCCAGTTTCT CTGTGGCTTA CGCAAACATG
    TGTCGATGTC TAGTAACGCT GAAAGTACCC ATGGCAGACA AGCCTGGTAA CACAGTGAAC
    TTCCGGAAGC TACTACTGAA CCGTTGCCAG AAGGAATTTG AAAAAGATAA AGCCGATGAT
    GATGTCTTTG AGAAGAAGCA GAAAGAACTC GAGGCTGCCA GTGCTCCAGA GGAGAGGACA
    AGGCTTCATG ATGAACTGGA AGAAGCTAAA GATAAAGCCC GGCGGAGATC CATTGGCAAC
    ATCAAGTTCA TTGGAGAACT CTTTAAACTC AAAATGCTGA CTGAAGCCAT CATGCATGAC
    TGTGTGGTGA AGCTGCTGAA GAACCACGAT GAAGAATCCC TCGAGTGTCT GTGTCGCCTG
    CTCACCACAA TTGGCAAAGA CCTGGACTTT GAGAAAGCAA AGCCACGTAT GGACCAGTAT
    TTTAATCAGA TGGAGAAAAT TGTAAAAGAA AGAAAAACCT CATCTAGGAT TCGATTCATG
    CTTCAGGACG TAATAGATCT AAGGCTGTGT AATTGGGTAT CACGACGAGC AGATCAGGGG
    CCTAAAACTA TTGAACAGAT TCACAAAGAA GCTAAAATAG AAGAACAGGA AGAGCAGAGG
    AAGGTCCAAC AACTCATGAC CAAAGAGAAG AGAAGACCAG GTGTCCAGAG AGTGGATGAA
    GGTGGGTGGA ACACTGTTCA AGGGGCCAAG AATAGTCGGG TATTGGACCC CTCAAAATTT
    CTGAAAATCA CTAAGCCCAC AATTGATGAG AAAATTCAGC TGGTACCTAA AGCGCAGCTG
    GGCAGCTGGG GAAAGGGCAG CAGTGGTGGA GCCAAGGCGA GCGAGGCCGA TGCCTTAAGA
    TCAAGTGCTT CCAGTTTAAA CAGATTCTCT GCTTTGCAAC CTCCAGCACC TTCAGGGTCT
    GCGTCATCCA CACCTCTAGA GTTTGATTCC CGGCGGACCT TAACTAGTCG AGGAAGCACG
    GGCAGGGAGA AGAATGACAA GCCCCTCCAG CCTGCAGCAG CTCGGCCGAA CACTTTCATG
    AGGGGTGGCA GCAGTAAAGA CCTGTTAGAC AATCAGTCTC AGGAGGAGCA GCGGAGGGAG
    ATGCTGGAGA CCGTGAAGCA GCTCACAGGA GGCATGGACG TGGAGAGGAG CAGCGCTGAG
    GCGGAGCGAA GCAGGACCAG GGAGCCAGTG ACAAAACCGG AAATTCCAGT AACATCAGCC
    TCTGACAAGC CCGCGTTGTC AGAGGAAGAG ATGGAGAGGA AGTCCAAATC TATCATTGAT
    GAGTTTCTAC ACATTAATGA TTTTAAGGAA GCCATGCAGT GTGTGGAAGA GCTGAACGCC
    CAGGGCCTGC AACATGTGTT CGTGAGGACG GGAGTGGAGT CCACCCTGGA AAGGAGCCAG
    ATCACCCGGG ATCACATGGG CCAGTTACTG TATCAGCTGG TGCAGTCAGA AAAGCTCAGC
    AAACAGGACT TTTTCAGAGG TTTTTCAGAA ACATTGGAAT TGGCAGATGA CATGGCCATT
    GATATTCCCC ATATTTGGTT GTACCTTGCT GAACTGGTGA CTCCCATGTT AAAAGAAGGC
    GGAATCTCCA TGAGACAACT CATCACAGAA TTTTGCAAAC CTTTACTTCC TGTTGGAAGA
    GCTGGAATCT TGATTTCTGA AATATTGCAC CTTCTATGCA AACAAATGAG CCATAAGAAA
    GTGGGAGCCC TATGGAGGGA GGCTGACCTC AGCTGGAAGG ACTTTTTACC AGAAGGGGAA
    GATGTACATA ATTTTCTCTT GGAGCAGAAG TTGGACTTTT TAGAGTCTGA CAGTTCCTGT
    TCCTCCGAAG CACTTTTGAA GAAAGAACTC TCTGCCGAAG AGCTGTATAA GCGACTCGAG
    AAACTCATTA TTGAGGACAA GGCAAATGAT GAACAGATCT TTGACTGGGT AGAGGCTAAT
    CTAGATGAAA GCCAGATGAG TTCACCTACA TTCCTTAGAG CTTTAATGAC AGCCGTCTGT
    AAAGCAGCTA TTATAGCCGA CTGTTCGACC TTCCGAGTGG ACACTGCTGT TATCAAGCAG
    AGAGTGCCGA TCTTACTCAA GTACCTAGAC TCAGATACAG AGAAGGAACT GCAAGCACTT
    TATGCACTAC AAGCATCAAT AGTAAAACTT GATCAACCTG CCAATTTGCT ACGGATGTTT
    TTTGATTGCC TGTATGACGA GGAGGTGATC TCTGAGGATG CCTTCTACAA GTGGGAGAGC
    AGCAAGGACC CGGCAGAGCA GAATGGGAAG GGTGTGGCCC TGAAGTCTGT CACGGCGTTC
    TTCACATGGC TGCGGGAAGC AGAAGAGGAG TCTGAGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03120
    Clone ID Related Accession (Same CDS sequence) XM_015460883.2 , XM_015462479.1 , XM_024982991.1 , XM_015460883.1
    Accession Version XM_015462479.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGTTTTTCC AGAGGCCTCA AATACAGCCT CCCAGAGCCA CCATCCCGAA CAGCAGCCCT
    TCCATTCGAC CCGGTGCACA GACACCCACT GCAGTGTATC AGGCCAATCA GCACATCATG
    ATGGTTAACC ATCTGCCCAT GCCGTACCCA GTGCCCCAGG GTCCTCAGTA CTGTATACCA
    CAGTACCGTC ACAGTGGCCC TCCGTATGTT GGGCCACCAC AACAGTACCC GGTTCAACCA
    CCAGGACCAG GTCCTTTTTA TCCTGGGCCA GGACCTGGGG ACTTCCCCAG TGCTTACGGA
    ACGCCTTTTT ACCCAAGTCA GCCAGTGTAC CAGTCAGCAC CTATCATAGT GCCTACGCAG
    CAGCAGCCCC CTCCAGCCAA GAGAGAGAAA AAAACAATAA GAATTCGGGA TCCAAACCAG
    GGAGGTAAAG ACATAACAGA GGAGATTATG TCTGGAGGTG GCAGCCGAAA TCCTACTCCA
    CCCATAGGGA GACCCACTTC CACACCTACT CCTCCTCAGC AGCTGCCCAG CCAGGTCCCC
    GAGCACAGCC CTGTGGTCTA TGGGACTGTG GAGAGCGCTC ATCTTGCTGC CAGCACCCCT
    GTCACTGCAG CGAGCGACCA GAAGCAAGAA GAGAAGCCAA AACCAGATCC AGTGTTAAAG
    ACTCCTTCCC CAGTCCTTAG GCTAGCCCTC AGTGGAGAGA AGAAAGAACA AGCAGGCCCG
    ACGTCTGAGA TTACTGCAGT AGAGTCCATA CCAGAGCTTC CTCTGCCTCC ATCCCCTACT
    GCTATTTCTC CACTTGCTCG AAGTACAATT ACTTCTCCCA CCTCTGTTGC TCTTAGTAGC
    CAACCAATAT TCACCACTGC AATTGATGAC AGATGTGAAC TCTCTTCCTC AAAAGAAGAT
    ACAATTCCTA TTCCCAGCCC CACATCTTGC ACAGAAGCAT CAGATCCTTT ACCAACAGAT
    GAAATTGATG ATGATATATG CAAGAAATCT TGTAGTGTAG CACCAAATGA TATTCCACTG
    ATTTCTAGTA CTAACCTAAT TAATGAAATG AATGGAGTTA ATGAAAAATT ACCAGCCACG
    GAAAGCATTG TGGAAATAGT AAAACAGGAG GTGTTACCGC TGACTCTTGA ATTGGAGATT
    TTGGAAAATC CCCCTGAAGA AATCAAAGTG GAGTGTGTCC CAACTCCCAT CACCCCTTCG
    ATAGTTCCCT CCTCTTCTCC GTCTCCTCCA GCTCCTCCAG CTTCTCCTTC TCCCACACCA
    GTTATCGTTC CTGCTGCTGC CACCAGTGTT AATACTGGTC GTGGTTCCAC CGGAGTCCAG
    AGAGCCTTCG AAGAGGGCGA GAACATAAGA ACTTGCCTTA GTGAAGATGC AAAAGAGATT
    CAGAACAAAG TAGAGGTAGA AGCAGATGGG CAAACAGAAG AGATTGTGGA TTCTCAGAAC
    TTAAGTTCAA GAAAGAGCCC AGTCCCAGCT CAGACAGCTA TACCTGCACC AAAGATGTGG
    AAGAAACCAA AAGACCGGAC CCGAGCCACT GAAGAGTCGT TAGAGGCAGA ATCAGAGCCT
    AAAGCTGAAG AGGAGCTTTC AGGTGACAAA ATACTTGAAT CTGAACAGGA TAAAATGAGC
    CAAGGGTTTC ATCCTGAAAG AGACCCCTCT GACCTAAAAA ACGTGAAAAC TGTGGAAGAA
    AATGGAGAAG AAGCTGAGCC TGTACGTAAT GGTGCTGAGA GTATTTCTGA GGGTGAAGGA
    ATAGATGCTA ATTCAGGCTC CACTGATAGT TCTGGTGAAG GGGTCACATT CCCATTTAAG
    TCAGAATCCT GGAAGCCTCC TGATTCGGAA GGCAAGAAGC AGTATGACAG GGAGTTTTTG
    CTGGATTTCC AATTCATGCC AGCGTGTATT CAGAAACCAG AGGGCCTGCC TCCCATCAGT
    GATGTGGTTC TTGACAAGAT CAATCAACCA AAATTGCCAA TGCGAACTCT GGATCCTCGG
    ATTTTGCCTC GAGGACCAGA CTTTACTCCA GCCTTTGCAG ATTTTGGAAG GCAAGCCCCT
    GGTGGAAGAG GTGTACCTGT TTGCAAAGTG CAGAGCAGGC ATGGATTGCC AATTCTGGAA
    CAGAGCAAAG CCCCAGCTTC CACTCCACTG GCGATGTCGC ACCCACCCCT GAAGAGCCTG
    CCTCTAGGGC TGTTGAATGT TGGAGCACGG AGATCTCAGC CTGGCCAGAG GAGAGAGCCC
    AGGAAGATCA TCACGGTCTC CGTGAAAGAA GACGTGCACC TGAAGAAGGC AGAGAACGCC
    TGGAAGCCCA GCCAGAGGCG GGATAGCCCA GCCGAGGACC CCGAGAACAT CAAGACCCAG
    GAACTTTTTA GAAAAGTTCG AAGTATCTTA AATAAATTGA CACCACAGAT GTTCAACCAA
    CTGATGAAGC AAGTGTCAGG ACTTACTGTT GACACAGAGG AGCGGCTGAA AGGAGTCATT
    GACCTGGTCT TTGAGAAGGC TATTGACGAA CCCAGTTTCT CTGTGGCTTA CGCAAACATG
    TGTCGATGTC TAGTAACGCT GAAAGTACCC ATGGCAGACA AGCCTGGTAA CACAGTGAAC
    TTCCGGAAGC TACTACTGAA CCGTTGCCAG AAGGAATTTG AAAAAGATAA AGCCGATGAT
    GATGTCTTTG AGAAGAAGCA GAAAGAACTC GAGGCTGCCA GTGCTCCAGA GGAGAGGACA
    AGGCTTCATG ATGAACTGGA AGAAGCTAAA GATAAAGCCC GGCGGAGATC CATTGGCAAC
    ATCAAGTTCA TTGGAGAACT CTTTAAACTC AAAATGCTGA CTGAAGCCAT CATGCATGAC
    TGTGTGGTGA AGCTGCTGAA GAACCACGAT GAAGAATCCC TCGAGTGTCT GTGTCGCCTG
    CTCACCACAA TTGGCAAAGA CCTGGACTTT GAGAAAGCAA AGCCACGTAT GGACCAGTAT
    TTTAATCAGA TGGAGAAAAT TGTAAAAGAA AGAAAAACCT CATCTAGGAT TCGATTCATG
    CTTCAGGACG TAATAGATCT AAGGCTGTGT AATTGGGTAT CACGACGAGC AGATCAGGGG
    CCTAAAACTA TTGAACAGAT TCACAAAGAA GCTAAAATAG AAGAACAGGA AGAGCAGAGG
    AAGGTCCAAC AACTCATGAC CAAAGAGAAG AGAAGACCAG GTGTCCAGAG AGTGGATGAA
    GGTGGGTGGA ACACTGTTCA AGGGGCCAAG AATAGTCGGG TATTGGACCC CTCAAAATTT
    CTGAAAATCA CTAAGCCCAC AATTGATGAG AAAATTCAGC TGGTACCTAA AGCGCAGCTG
    GGCAGCTGGG GAAAGGGCAG CAGTGGTGGA GCCAAGGCGA GCGAGGCCGA TGCCTTAAGA
    TCAAGTGCTT CCAGTTTAAA CAGATTCTCT GCTTTGCAAC CTCCAGCACC TTCAGGGTCT
    GCGTCATCCA CACCTCTAGA GTTTGATTCC CGGCGGACCT TAACTAGTCG AGGAAGCACG
    GGCAGGGAGA AGAATGACAA GCCCCTCCAG CCTGCAGCAG CTCGGCCGAA CACTTTCATG
    AGGGGTGGCA GCAGTAAAGA CCTGTTAGAC AATCAGTCTC AGGAGGAGCA GCGGAGGGAG
    ATGCTGGAGA CCGTGAAGCA GCTCACAGGA GGCATGGACG TGGAGAGGAG CAGCGCTGAG
    GCGGAGCGAA GCAGGACCAG GGAGCCAGTG ACAAAACCGG AAATTCCAGT AACATCAGCC
    TCTGACAAGC CCGCGTTGTC AGAGGAAGAG ATGGAGAGGA AGTCCAAATC TATCATTGAT
    GAGTTTCTAC ACATTAATGA TTTTAAGGAA GCCATGCAGT GTGTGGAAGA GCTGAACGCC
    CAGGGCCTGC AACATGTGTT CGTGAGGACG GGAGTGGAGT CCACCCTGGA AAGGAGCCAG
    ATCACCCGGG ATCACATGGG CCAGTTACTG TATCAGCTGG TGCAGTCAGA AAAGCTCAGC
    AAACAGGACT TTTTCAGAGG TTTTTCAGAA ACATTGGAAT TGGCAGATGA CATGGCCATT
    GATATTCCCC ATATTTGGTT GTACCTTGCT GAACTGGTGA CTCCCATGTT AAAAGAAGGC
    GGAATCTCCA TGAGACAACT CATCACAGAA TTTTGCAAAC CTTTACTTCC TGTTGGAAGA
    GCTGGAATCT TGATTTCTGA AATATTGCAC CTTCTATGCA AACAAATGAG CCATAAGAAA
    GTGGGAGCCC TATGGAGGGA GGCTGACCTC AGCTGGAAGG ACTTTTTACC AGAAGGGGAA
    GATGTACATA ATTTTCTCTT GGAGCAGAAG TTGGACTTTT TAGAGTCTGA CAGTTCCTGT
    TCCTCCGAAG CACTTTTGAA GAAAGAACTC TCTGCCGAAG AGCTGTATAA GCGACTCGAG
    AAACTCATTA TTGAGGACAA GGCAAATGAT GAACAGATCT TTGACTGGGT AGAGGCTAAT
    CTAGATGAAA GCCAGATGAG TTCACCTACA TTCCTTAGAG CTTTAATGAC AGCCGTCTGT
    AAAGCAGCTA TTATAGCCGA CTGTTCGACC TTCCGAGTGG ACACTGCTGT TATCAAGCAG
    AGAGTGCCGA TCTTACTCAA GTACCTAGAC TCAGATACAG AGAAGGAACT GCAAGCACTT
    TATGCACTAC AAGCATCAAT AGTAAAACTT GATCAACCTG CCAATTTGCT ACGGATGTTT
    TTTGATTGCC TGTATGACGA GGAGGTGATC TCTGAGGATG CCTTCTACAA GTGGGAGAGC
    AGCAAGGACC CGGCAGAGCA GAATGGGAAG GGTGTGGCCC TGAAGTCTGT CACGGCGTTC
    TTCACATGGC TGCGGGAAGC AGAAGAGGAG TCTGAGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317965.1
    CDS190..5094
    Translation

    Target ORF information:

    RefSeq Version XM_015462479.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462479.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGTTTTTCC AGAGGCCTCA AATACAGCCT CCCAGAGCCA CCATCCCGAA CAGCAGCCCT
    TCCATTCGAC CCGGTGCACA GACACCCACT GCAGTGTATC AGGCCAATCA GCACATCATG
    ATGGTTAACC ATCTGCCCAT GCCGTACCCA GTGCCCCAGG GTCCTCAGTA CTGTATACCA
    CAGTACCGTC ACAGTGGCCC TCCGTATGTT GGGCCACCAC AACAGTACCC GGTTCAACCA
    CCAGGACCAG GTCCTTTTTA TCCTGGGCCA GGACCTGGGG ACTTCCCCAG TGCTTACGGA
    ACGCCTTTTT ACCCAAGTCA GCCAGTGTAC CAGTCAGCAC CTATCATAGT GCCTACGCAG
    CAGCAGCCCC CTCCAGCCAA GAGAGAGAAA AAAACAATAA GAATTCGGGA TCCAAACCAG
    GGAGGTAAAG ACATAACAGA GGAGATTATG TCTGGAGGTG GCAGCCGAAA TCCTACTCCA
    CCCATAGGGA GACCCACTTC CACACCTACT CCTCCTCAGC AGCTGCCCAG CCAGGTCCCC
    GAGCACAGCC CTGTGGTCTA TGGGACTGTG GAGAGCGCTC ATCTTGCTGC CAGCACCCCT
    GTCACTGCAG CGAGCGACCA GAAGCAAGAA GAGAAGCCAA AACCAGATCC AGTGTTAAAG
    ACTCCTTCCC CAGTCCTTAG GCTAGCCCTC AGTGGAGAGA AGAAAGAACA AGCAGGCCCG
    ACGTCTGAGA TTACTGCAGT AGAGTCCATA CCAGAGCTTC CTCTGCCTCC ATCCCCTACT
    GCTATTTCTC CACTTGCTCG AAGTACAATT ACTTCTCCCA CCTCTGTTGC TCTTAGTAGC
    CAACCAATAT TCACCACTGC AATTGATGAC AGATGTGAAC TCTCTTCCTC AAAAGAAGAT
    ACAATTCCTA TTCCCAGCCC CACATCTTGC ACAGAAGCAT CAGATCCTTT ACCAACAGAT
    GAAATTGATG ATGATATATG CAAGAAATCT TGTAGTGTAG CACCAAATGA TATTCCACTG
    ATTTCTAGTA CTAACCTAAT TAATGAAATG AATGGAGTTA ATGAAAAATT ACCAGCCACG
    GAAAGCATTG TGGAAATAGT AAAACAGGAG GTGTTACCGC TGACTCTTGA ATTGGAGATT
    TTGGAAAATC CCCCTGAAGA AATCAAAGTG GAGTGTGTCC CAACTCCCAT CACCCCTTCG
    ATAGTTCCCT CCTCTTCTCC GTCTCCTCCA GCTCCTCCAG CTTCTCCTTC TCCCACACCA
    GTTATCGTTC CTGCTGCTGC CACCAGTGTT AATACTGGTC GTGGTTCCAC CGGAGTCCAG
    AGAGCCTTCG AAGAGGGCGA GAACATAAGA ACTTGCCTTA GTGAAGATGC AAAAGAGATT
    CAGAACAAAG TAGAGGTAGA AGCAGATGGG CAAACAGAAG AGATTGTGGA TTCTCAGAAC
    TTAAGTTCAA GAAAGAGCCC AGTCCCAGCT CAGACAGCTA TACCTGCACC AAAGATGTGG
    AAGAAACCAA AAGACCGGAC CCGAGCCACT GAAGAGTCGT TAGAGGCAGA ATCAGAGCCT
    AAAGCTGAAG AGGAGCTTTC AGGTGACAAA ATACTTGAAT CTGAACAGGA TAAAATGAGC
    CAAGGGTTTC ATCCTGAAAG AGACCCCTCT GACCTAAAAA ACGTGAAAAC TGTGGAAGAA
    AATGGAGAAG AAGCTGAGCC TGTACGTAAT GGTGCTGAGA GTATTTCTGA GGGTGAAGGA
    ATAGATGCTA ATTCAGGCTC CACTGATAGT TCTGGTGAAG GGGTCACATT CCCATTTAAG
    TCAGAATCCT GGAAGCCTCC TGATTCGGAA GGCAAGAAGC AGTATGACAG GGAGTTTTTG
    CTGGATTTCC AATTCATGCC AGCGTGTATT CAGAAACCAG AGGGCCTGCC TCCCATCAGT
    GATGTGGTTC TTGACAAGAT CAATCAACCA AAATTGCCAA TGCGAACTCT GGATCCTCGG
    ATTTTGCCTC GAGGACCAGA CTTTACTCCA GCCTTTGCAG ATTTTGGAAG GCAAGCCCCT
    GGTGGAAGAG GTGTACCTGT TTGCAAAGTG CAGAGCAGGC ATGGATTGCC AATTCTGGAA
    CAGAGCAAAG CCCCAGCTTC CACTCCACTG GCGATGTCGC ACCCACCCCT GAAGAGCCTG
    CCTCTAGGGC TGTTGAATGT TGGAGCACGG AGATCTCAGC CTGGCCAGAG GAGAGAGCCC
    AGGAAGATCA TCACGGTCTC CGTGAAAGAA GACGTGCACC TGAAGAAGGC AGAGAACGCC
    TGGAAGCCCA GCCAGAGGCG GGATAGCCCA GCCGAGGACC CCGAGAACAT CAAGACCCAG
    GAACTTTTTA GAAAAGTTCG AAGTATCTTA AATAAATTGA CACCACAGAT GTTCAACCAA
    CTGATGAAGC AAGTGTCAGG ACTTACTGTT GACACAGAGG AGCGGCTGAA AGGAGTCATT
    GACCTGGTCT TTGAGAAGGC TATTGACGAA CCCAGTTTCT CTGTGGCTTA CGCAAACATG
    TGTCGATGTC TAGTAACGCT GAAAGTACCC ATGGCAGACA AGCCTGGTAA CACAGTGAAC
    TTCCGGAAGC TACTACTGAA CCGTTGCCAG AAGGAATTTG AAAAAGATAA AGCCGATGAT
    GATGTCTTTG AGAAGAAGCA GAAAGAACTC GAGGCTGCCA GTGCTCCAGA GGAGAGGACA
    AGGCTTCATG ATGAACTGGA AGAAGCTAAA GATAAAGCCC GGCGGAGATC CATTGGCAAC
    ATCAAGTTCA TTGGAGAACT CTTTAAACTC AAAATGCTGA CTGAAGCCAT CATGCATGAC
    TGTGTGGTGA AGCTGCTGAA GAACCACGAT GAAGAATCCC TCGAGTGTCT GTGTCGCCTG
    CTCACCACAA TTGGCAAAGA CCTGGACTTT GAGAAAGCAA AGCCACGTAT GGACCAGTAT
    TTTAATCAGA TGGAGAAAAT TGTAAAAGAA AGAAAAACCT CATCTAGGAT TCGATTCATG
    CTTCAGGACG TAATAGATCT AAGGCTGTGT AATTGGGTAT CACGACGAGC AGATCAGGGG
    CCTAAAACTA TTGAACAGAT TCACAAAGAA GCTAAAATAG AAGAACAGGA AGAGCAGAGG
    AAGGTCCAAC AACTCATGAC CAAAGAGAAG AGAAGACCAG GTGTCCAGAG AGTGGATGAA
    GGTGGGTGGA ACACTGTTCA AGGGGCCAAG AATAGTCGGG TATTGGACCC CTCAAAATTT
    CTGAAAATCA CTAAGCCCAC AATTGATGAG AAAATTCAGC TGGTACCTAA AGCGCAGCTG
    GGCAGCTGGG GAAAGGGCAG CAGTGGTGGA GCCAAGGCGA GCGAGGCCGA TGCCTTAAGA
    TCAAGTGCTT CCAGTTTAAA CAGATTCTCT GCTTTGCAAC CTCCAGCACC TTCAGGGTCT
    GCGTCATCCA CACCTCTAGA GTTTGATTCC CGGCGGACCT TAACTAGTCG AGGAAGCACG
    GGCAGGGAGA AGAATGACAA GCCCCTCCAG CCTGCAGCAG CTCGGCCGAA CACTTTCATG
    AGGGGTGGCA GCAGTAAAGA CCTGTTAGAC AATCAGTCTC AGGAGGAGCA GCGGAGGGAG
    ATGCTGGAGA CCGTGAAGCA GCTCACAGGA GGCATGGACG TGGAGAGGAG CAGCGCTGAG
    GCGGAGCGAA GCAGGACCAG GGAGCCAGTG ACAAAACCGG AAATTCCAGT AACATCAGCC
    TCTGACAAGC CCGCGTTGTC AGAGGAAGAG ATGGAGAGGA AGTCCAAATC TATCATTGAT
    GAGTTTCTAC ACATTAATGA TTTTAAGGAA GCCATGCAGT GTGTGGAAGA GCTGAACGCC
    CAGGGCCTGC AACATGTGTT CGTGAGGACG GGAGTGGAGT CCACCCTGGA AAGGAGCCAG
    ATCACCCGGG ATCACATGGG CCAGTTACTG TATCAGCTGG TGCAGTCAGA AAAGCTCAGC
    AAACAGGACT TTTTCAGAGG TTTTTCAGAA ACATTGGAAT TGGCAGATGA CATGGCCATT
    GATATTCCCC ATATTTGGTT GTACCTTGCT GAACTGGTGA CTCCCATGTT AAAAGAAGGC
    GGAATCTCCA TGAGACAACT CATCACAGAA TTTTGCAAAC CTTTACTTCC TGTTGGAAGA
    GCTGGAATCT TGATTTCTGA AATATTGCAC CTTCTATGCA AACAAATGAG CCATAAGAAA
    GTGGGAGCCC TATGGAGGGA GGCTGACCTC AGCTGGAAGG ACTTTTTACC AGAAGGGGAA
    GATGTACATA ATTTTCTCTT GGAGCAGAAG TTGGACTTTT TAGAGTCTGA CAGTTCCTGT
    TCCTCCGAAG CACTTTTGAA GAAAGAACTC TCTGCCGAAG AGCTGTATAA GCGACTCGAG
    AAACTCATTA TTGAGGACAA GGCAAATGAT GAACAGATCT TTGACTGGGT AGAGGCTAAT
    CTAGATGAAA GCCAGATGAG TTCACCTACA TTCCTTAGAG CTTTAATGAC AGCCGTCTGT
    AAAGCAGCTA TTATAGCCGA CTGTTCGACC TTCCGAGTGG ACACTGCTGT TATCAAGCAG
    AGAGTGCCGA TCTTACTCAA GTACCTAGAC TCAGATACAG AGAAGGAACT GCAAGCACTT
    TATGCACTAC AAGCATCAAT AGTAAAACTT GATCAACCTG CCAATTTGCT ACGGATGTTT
    TTTGATTGCC TGTATGACGA GGAGGTGATC TCTGAGGATG CCTTCTACAA GTGGGAGAGC
    AGCAAGGACC CGGCAGAGCA GAATGGGAAG GGTGTGGCCC TGAAGTCTGT CACGGCGTTC
    TTCACATGGC TGCGGGAAGC AGAAGAGGAG TCTGAGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03120
    Clone ID Related Accession (Same CDS sequence) XM_015460883.2 , XM_015462479.1 , XM_024982991.1 , XM_015460883.1
    Accession Version XM_024982991.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGTTTTTCC AGAGGCCTCA AATACAGCCT CCCAGAGCCA CCATCCCGAA CAGCAGCCCT
    TCCATTCGAC CCGGTGCACA GACACCCACT GCAGTGTATC AGGCCAATCA GCACATCATG
    ATGGTTAACC ATCTGCCCAT GCCGTACCCA GTGCCCCAGG GTCCTCAGTA CTGTATACCA
    CAGTACCGTC ACAGTGGCCC TCCGTATGTT GGGCCACCAC AACAGTACCC GGTTCAACCA
    CCAGGACCAG GTCCTTTTTA TCCTGGGCCA GGACCTGGGG ACTTCCCCAG TGCTTACGGA
    ACGCCTTTTT ACCCAAGTCA GCCAGTGTAC CAGTCAGCAC CTATCATAGT GCCTACGCAG
    CAGCAGCCCC CTCCAGCCAA GAGAGAGAAA AAAACAATAA GAATTCGGGA TCCAAACCAG
    GGAGGTAAAG ACATAACAGA GGAGATTATG TCTGGAGGTG GCAGCCGAAA TCCTACTCCA
    CCCATAGGGA GACCCACTTC CACACCTACT CCTCCTCAGC AGCTGCCCAG CCAGGTCCCC
    GAGCACAGCC CTGTGGTCTA TGGGACTGTG GAGAGCGCTC ATCTTGCTGC CAGCACCCCT
    GTCACTGCAG CGAGCGACCA GAAGCAAGAA GAGAAGCCAA AACCAGATCC AGTGTTAAAG
    ACTCCTTCCC CAGTCCTTAG GCTAGCCCTC AGTGGAGAGA AGAAAGAACA AGCAGGCCCG
    ACGTCTGAGA TTACTGCAGT AGAGTCCATA CCAGAGCTTC CTCTGCCTCC ATCCCCTACT
    GCTATTTCTC CACTTGCTCG AAGTACAATT ACTTCTCCCA CCTCTGTTGC TCTTAGTAGC
    CAACCAATAT TCACCACTGC AATTGATGAC AGATGTGAAC TCTCTTCCTC AAAAGAAGAT
    ACAATTCCTA TTCCCAGCCC CACATCTTGC ACAGAAGCAT CAGATCCTTT ACCAACAGAT
    GAAATTGATG ATGATATATG CAAGAAATCT TGTAGTGTAG CACCAAATGA TATTCCACTG
    ATTTCTAGTA CTAACCTAAT TAATGAAATG AATGGAGTTA ATGAAAAATT ACCAGCCACG
    GAAAGCATTG TGGAAATAGT AAAACAGGAG GTGTTACCGC TGACTCTTGA ATTGGAGATT
    TTGGAAAATC CCCCTGAAGA AATCAAAGTG GAGTGTGTCC CAACTCCCAT CACCCCTTCG
    ATAGTTCCCT CCTCTTCTCC GTCTCCTCCA GCTCCTCCAG CTTCTCCTTC TCCCACACCA
    GTTATCGTTC CTGCTGCTGC CACCAGTGTT AATACTGGTC GTGGTTCCAC CGGAGTCCAG
    AGAGCCTTCG AAGAGGGCGA GAACATAAGA ACTTGCCTTA GTGAAGATGC AAAAGAGATT
    CAGAACAAAG TAGAGGTAGA AGCAGATGGG CAAACAGAAG AGATTGTGGA TTCTCAGAAC
    TTAAGTTCAA GAAAGAGCCC AGTCCCAGCT CAGACAGCTA TACCTGCACC AAAGATGTGG
    AAGAAACCAA AAGACCGGAC CCGAGCCACT GAAGAGTCGT TAGAGGCAGA ATCAGAGCCT
    AAAGCTGAAG AGGAGCTTTC AGGTGACAAA ATACTTGAAT CTGAACAGGA TAAAATGAGC
    CAAGGGTTTC ATCCTGAAAG AGACCCCTCT GACCTAAAAA ACGTGAAAAC TGTGGAAGAA
    AATGGAGAAG AAGCTGAGCC TGTACGTAAT GGTGCTGAGA GTATTTCTGA GGGTGAAGGA
    ATAGATGCTA ATTCAGGCTC CACTGATAGT TCTGGTGAAG GGGTCACATT CCCATTTAAG
    TCAGAATCCT GGAAGCCTCC TGATTCGGAA GGCAAGAAGC AGTATGACAG GGAGTTTTTG
    CTGGATTTCC AATTCATGCC AGCGTGTATT CAGAAACCAG AGGGCCTGCC TCCCATCAGT
    GATGTGGTTC TTGACAAGAT CAATCAACCA AAATTGCCAA TGCGAACTCT GGATCCTCGG
    ATTTTGCCTC GAGGACCAGA CTTTACTCCA GCCTTTGCAG ATTTTGGAAG GCAAGCCCCT
    GGTGGAAGAG GTGTACCTGT TTGCAAAGTG CAGAGCAGGC ATGGATTGCC AATTCTGGAA
    CAGAGCAAAG CCCCAGCTTC CACTCCACTG GCGATGTCGC ACCCACCCCT GAAGAGCCTG
    CCTCTAGGGC TGTTGAATGT TGGAGCACGG AGATCTCAGC CTGGCCAGAG GAGAGAGCCC
    AGGAAGATCA TCACGGTCTC CGTGAAAGAA GACGTGCACC TGAAGAAGGC AGAGAACGCC
    TGGAAGCCCA GCCAGAGGCG GGATAGCCCA GCCGAGGACC CCGAGAACAT CAAGACCCAG
    GAACTTTTTA GAAAAGTTCG AAGTATCTTA AATAAATTGA CACCACAGAT GTTCAACCAA
    CTGATGAAGC AAGTGTCAGG ACTTACTGTT GACACAGAGG AGCGGCTGAA AGGAGTCATT
    GACCTGGTCT TTGAGAAGGC TATTGACGAA CCCAGTTTCT CTGTGGCTTA CGCAAACATG
    TGTCGATGTC TAGTAACGCT GAAAGTACCC ATGGCAGACA AGCCTGGTAA CACAGTGAAC
    TTCCGGAAGC TACTACTGAA CCGTTGCCAG AAGGAATTTG AAAAAGATAA AGCCGATGAT
    GATGTCTTTG AGAAGAAGCA GAAAGAACTC GAGGCTGCCA GTGCTCCAGA GGAGAGGACA
    AGGCTTCATG ATGAACTGGA AGAAGCTAAA GATAAAGCCC GGCGGAGATC CATTGGCAAC
    ATCAAGTTCA TTGGAGAACT CTTTAAACTC AAAATGCTGA CTGAAGCCAT CATGCATGAC
    TGTGTGGTGA AGCTGCTGAA GAACCACGAT GAAGAATCCC TCGAGTGTCT GTGTCGCCTG
    CTCACCACAA TTGGCAAAGA CCTGGACTTT GAGAAAGCAA AGCCACGTAT GGACCAGTAT
    TTTAATCAGA TGGAGAAAAT TGTAAAAGAA AGAAAAACCT CATCTAGGAT TCGATTCATG
    CTTCAGGACG TAATAGATCT AAGGCTGTGT AATTGGGTAT CACGACGAGC AGATCAGGGG
    CCTAAAACTA TTGAACAGAT TCACAAAGAA GCTAAAATAG AAGAACAGGA AGAGCAGAGG
    AAGGTCCAAC AACTCATGAC CAAAGAGAAG AGAAGACCAG GTGTCCAGAG AGTGGATGAA
    GGTGGGTGGA ACACTGTTCA AGGGGCCAAG AATAGTCGGG TATTGGACCC CTCAAAATTT
    CTGAAAATCA CTAAGCCCAC AATTGATGAG AAAATTCAGC TGGTACCTAA AGCGCAGCTG
    GGCAGCTGGG GAAAGGGCAG CAGTGGTGGA GCCAAGGCGA GCGAGGCCGA TGCCTTAAGA
    TCAAGTGCTT CCAGTTTAAA CAGATTCTCT GCTTTGCAAC CTCCAGCACC TTCAGGGTCT
    GCGTCATCCA CACCTCTAGA GTTTGATTCC CGGCGGACCT TAACTAGTCG AGGAAGCACG
    GGCAGGGAGA AGAATGACAA GCCCCTCCAG CCTGCAGCAG CTCGGCCGAA CACTTTCATG
    AGGGGTGGCA GCAGTAAAGA CCTGTTAGAC AATCAGTCTC AGGAGGAGCA GCGGAGGGAG
    ATGCTGGAGA CCGTGAAGCA GCTCACAGGA GGCATGGACG TGGAGAGGAG CAGCGCTGAG
    GCGGAGCGAA GCAGGACCAG GGAGCCAGTG ACAAAACCGG AAATTCCAGT AACATCAGCC
    TCTGACAAGC CCGCGTTGTC AGAGGAAGAG ATGGAGAGGA AGTCCAAATC TATCATTGAT
    GAGTTTCTAC ACATTAATGA TTTTAAGGAA GCCATGCAGT GTGTGGAAGA GCTGAACGCC
    CAGGGCCTGC AACATGTGTT CGTGAGGACG GGAGTGGAGT CCACCCTGGA AAGGAGCCAG
    ATCACCCGGG ATCACATGGG CCAGTTACTG TATCAGCTGG TGCAGTCAGA AAAGCTCAGC
    AAACAGGACT TTTTCAGAGG TTTTTCAGAA ACATTGGAAT TGGCAGATGA CATGGCCATT
    GATATTCCCC ATATTTGGTT GTACCTTGCT GAACTGGTGA CTCCCATGTT AAAAGAAGGC
    GGAATCTCCA TGAGACAACT CATCACAGAA TTTTGCAAAC CTTTACTTCC TGTTGGAAGA
    GCTGGAATCT TGATTTCTGA AATATTGCAC CTTCTATGCA AACAAATGAG CCATAAGAAA
    GTGGGAGCCC TATGGAGGGA GGCTGACCTC AGCTGGAAGG ACTTTTTACC AGAAGGGGAA
    GATGTACATA ATTTTCTCTT GGAGCAGAAG TTGGACTTTT TAGAGTCTGA CAGTTCCTGT
    TCCTCCGAAG CACTTTTGAA GAAAGAACTC TCTGCCGAAG AGCTGTATAA GCGACTCGAG
    AAACTCATTA TTGAGGACAA GGCAAATGAT GAACAGATCT TTGACTGGGT AGAGGCTAAT
    CTAGATGAAA GCCAGATGAG TTCACCTACA TTCCTTAGAG CTTTAATGAC AGCCGTCTGT
    AAAGCAGCTA TTATAGCCGA CTGTTCGACC TTCCGAGTGG ACACTGCTGT TATCAAGCAG
    AGAGTGCCGA TCTTACTCAA GTACCTAGAC TCAGATACAG AGAAGGAACT GCAAGCACTT
    TATGCACTAC AAGCATCAAT AGTAAAACTT GATCAACCTG CCAATTTGCT ACGGATGTTT
    TTTGATTGCC TGTATGACGA GGAGGTGATC TCTGAGGATG CCTTCTACAA GTGGGAGAGC
    AGCAAGGACC CGGCAGAGCA GAATGGGAAG GGTGTGGCCC TGAAGTCTGT CACGGCGTTC
    TTCACATGGC TGCGGGAAGC AGAAGAGGAG TCTGAGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838759.1
    CDS494..5398
    Translation

    Target ORF information:

    RefSeq Version XM_024982991.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982991.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGTTTTTCC AGAGGCCTCA AATACAGCCT CCCAGAGCCA CCATCCCGAA CAGCAGCCCT
    TCCATTCGAC CCGGTGCACA GACACCCACT GCAGTGTATC AGGCCAATCA GCACATCATG
    ATGGTTAACC ATCTGCCCAT GCCGTACCCA GTGCCCCAGG GTCCTCAGTA CTGTATACCA
    CAGTACCGTC ACAGTGGCCC TCCGTATGTT GGGCCACCAC AACAGTACCC GGTTCAACCA
    CCAGGACCAG GTCCTTTTTA TCCTGGGCCA GGACCTGGGG ACTTCCCCAG TGCTTACGGA
    ACGCCTTTTT ACCCAAGTCA GCCAGTGTAC CAGTCAGCAC CTATCATAGT GCCTACGCAG
    CAGCAGCCCC CTCCAGCCAA GAGAGAGAAA AAAACAATAA GAATTCGGGA TCCAAACCAG
    GGAGGTAAAG ACATAACAGA GGAGATTATG TCTGGAGGTG GCAGCCGAAA TCCTACTCCA
    CCCATAGGGA GACCCACTTC CACACCTACT CCTCCTCAGC AGCTGCCCAG CCAGGTCCCC
    GAGCACAGCC CTGTGGTCTA TGGGACTGTG GAGAGCGCTC ATCTTGCTGC CAGCACCCCT
    GTCACTGCAG CGAGCGACCA GAAGCAAGAA GAGAAGCCAA AACCAGATCC AGTGTTAAAG
    ACTCCTTCCC CAGTCCTTAG GCTAGCCCTC AGTGGAGAGA AGAAAGAACA AGCAGGCCCG
    ACGTCTGAGA TTACTGCAGT AGAGTCCATA CCAGAGCTTC CTCTGCCTCC ATCCCCTACT
    GCTATTTCTC CACTTGCTCG AAGTACAATT ACTTCTCCCA CCTCTGTTGC TCTTAGTAGC
    CAACCAATAT TCACCACTGC AATTGATGAC AGATGTGAAC TCTCTTCCTC AAAAGAAGAT
    ACAATTCCTA TTCCCAGCCC CACATCTTGC ACAGAAGCAT CAGATCCTTT ACCAACAGAT
    GAAATTGATG ATGATATATG CAAGAAATCT TGTAGTGTAG CACCAAATGA TATTCCACTG
    ATTTCTAGTA CTAACCTAAT TAATGAAATG AATGGAGTTA ATGAAAAATT ACCAGCCACG
    GAAAGCATTG TGGAAATAGT AAAACAGGAG GTGTTACCGC TGACTCTTGA ATTGGAGATT
    TTGGAAAATC CCCCTGAAGA AATCAAAGTG GAGTGTGTCC CAACTCCCAT CACCCCTTCG
    ATAGTTCCCT CCTCTTCTCC GTCTCCTCCA GCTCCTCCAG CTTCTCCTTC TCCCACACCA
    GTTATCGTTC CTGCTGCTGC CACCAGTGTT AATACTGGTC GTGGTTCCAC CGGAGTCCAG
    AGAGCCTTCG AAGAGGGCGA GAACATAAGA ACTTGCCTTA GTGAAGATGC AAAAGAGATT
    CAGAACAAAG TAGAGGTAGA AGCAGATGGG CAAACAGAAG AGATTGTGGA TTCTCAGAAC
    TTAAGTTCAA GAAAGAGCCC AGTCCCAGCT CAGACAGCTA TACCTGCACC AAAGATGTGG
    AAGAAACCAA AAGACCGGAC CCGAGCCACT GAAGAGTCGT TAGAGGCAGA ATCAGAGCCT
    AAAGCTGAAG AGGAGCTTTC AGGTGACAAA ATACTTGAAT CTGAACAGGA TAAAATGAGC
    CAAGGGTTTC ATCCTGAAAG AGACCCCTCT GACCTAAAAA ACGTGAAAAC TGTGGAAGAA
    AATGGAGAAG AAGCTGAGCC TGTACGTAAT GGTGCTGAGA GTATTTCTGA GGGTGAAGGA
    ATAGATGCTA ATTCAGGCTC CACTGATAGT TCTGGTGAAG GGGTCACATT CCCATTTAAG
    TCAGAATCCT GGAAGCCTCC TGATTCGGAA GGCAAGAAGC AGTATGACAG GGAGTTTTTG
    CTGGATTTCC AATTCATGCC AGCGTGTATT CAGAAACCAG AGGGCCTGCC TCCCATCAGT
    GATGTGGTTC TTGACAAGAT CAATCAACCA AAATTGCCAA TGCGAACTCT GGATCCTCGG
    ATTTTGCCTC GAGGACCAGA CTTTACTCCA GCCTTTGCAG ATTTTGGAAG GCAAGCCCCT
    GGTGGAAGAG GTGTACCTGT TTGCAAAGTG CAGAGCAGGC ATGGATTGCC AATTCTGGAA
    CAGAGCAAAG CCCCAGCTTC CACTCCACTG GCGATGTCGC ACCCACCCCT GAAGAGCCTG
    CCTCTAGGGC TGTTGAATGT TGGAGCACGG AGATCTCAGC CTGGCCAGAG GAGAGAGCCC
    AGGAAGATCA TCACGGTCTC CGTGAAAGAA GACGTGCACC TGAAGAAGGC AGAGAACGCC
    TGGAAGCCCA GCCAGAGGCG GGATAGCCCA GCCGAGGACC CCGAGAACAT CAAGACCCAG
    GAACTTTTTA GAAAAGTTCG AAGTATCTTA AATAAATTGA CACCACAGAT GTTCAACCAA
    CTGATGAAGC AAGTGTCAGG ACTTACTGTT GACACAGAGG AGCGGCTGAA AGGAGTCATT
    GACCTGGTCT TTGAGAAGGC TATTGACGAA CCCAGTTTCT CTGTGGCTTA CGCAAACATG
    TGTCGATGTC TAGTAACGCT GAAAGTACCC ATGGCAGACA AGCCTGGTAA CACAGTGAAC
    TTCCGGAAGC TACTACTGAA CCGTTGCCAG AAGGAATTTG AAAAAGATAA AGCCGATGAT
    GATGTCTTTG AGAAGAAGCA GAAAGAACTC GAGGCTGCCA GTGCTCCAGA GGAGAGGACA
    AGGCTTCATG ATGAACTGGA AGAAGCTAAA GATAAAGCCC GGCGGAGATC CATTGGCAAC
    ATCAAGTTCA TTGGAGAACT CTTTAAACTC AAAATGCTGA CTGAAGCCAT CATGCATGAC
    TGTGTGGTGA AGCTGCTGAA GAACCACGAT GAAGAATCCC TCGAGTGTCT GTGTCGCCTG
    CTCACCACAA TTGGCAAAGA CCTGGACTTT GAGAAAGCAA AGCCACGTAT GGACCAGTAT
    TTTAATCAGA TGGAGAAAAT TGTAAAAGAA AGAAAAACCT CATCTAGGAT TCGATTCATG
    CTTCAGGACG TAATAGATCT AAGGCTGTGT AATTGGGTAT CACGACGAGC AGATCAGGGG
    CCTAAAACTA TTGAACAGAT TCACAAAGAA GCTAAAATAG AAGAACAGGA AGAGCAGAGG
    AAGGTCCAAC AACTCATGAC CAAAGAGAAG AGAAGACCAG GTGTCCAGAG AGTGGATGAA
    GGTGGGTGGA ACACTGTTCA AGGGGCCAAG AATAGTCGGG TATTGGACCC CTCAAAATTT
    CTGAAAATCA CTAAGCCCAC AATTGATGAG AAAATTCAGC TGGTACCTAA AGCGCAGCTG
    GGCAGCTGGG GAAAGGGCAG CAGTGGTGGA GCCAAGGCGA GCGAGGCCGA TGCCTTAAGA
    TCAAGTGCTT CCAGTTTAAA CAGATTCTCT GCTTTGCAAC CTCCAGCACC TTCAGGGTCT
    GCGTCATCCA CACCTCTAGA GTTTGATTCC CGGCGGACCT TAACTAGTCG AGGAAGCACG
    GGCAGGGAGA AGAATGACAA GCCCCTCCAG CCTGCAGCAG CTCGGCCGAA CACTTTCATG
    AGGGGTGGCA GCAGTAAAGA CCTGTTAGAC AATCAGTCTC AGGAGGAGCA GCGGAGGGAG
    ATGCTGGAGA CCGTGAAGCA GCTCACAGGA GGCATGGACG TGGAGAGGAG CAGCGCTGAG
    GCGGAGCGAA GCAGGACCAG GGAGCCAGTG ACAAAACCGG AAATTCCAGT AACATCAGCC
    TCTGACAAGC CCGCGTTGTC AGAGGAAGAG ATGGAGAGGA AGTCCAAATC TATCATTGAT
    GAGTTTCTAC ACATTAATGA TTTTAAGGAA GCCATGCAGT GTGTGGAAGA GCTGAACGCC
    CAGGGCCTGC AACATGTGTT CGTGAGGACG GGAGTGGAGT CCACCCTGGA AAGGAGCCAG
    ATCACCCGGG ATCACATGGG CCAGTTACTG TATCAGCTGG TGCAGTCAGA AAAGCTCAGC
    AAACAGGACT TTTTCAGAGG TTTTTCAGAA ACATTGGAAT TGGCAGATGA CATGGCCATT
    GATATTCCCC ATATTTGGTT GTACCTTGCT GAACTGGTGA CTCCCATGTT AAAAGAAGGC
    GGAATCTCCA TGAGACAACT CATCACAGAA TTTTGCAAAC CTTTACTTCC TGTTGGAAGA
    GCTGGAATCT TGATTTCTGA AATATTGCAC CTTCTATGCA AACAAATGAG CCATAAGAAA
    GTGGGAGCCC TATGGAGGGA GGCTGACCTC AGCTGGAAGG ACTTTTTACC AGAAGGGGAA
    GATGTACATA ATTTTCTCTT GGAGCAGAAG TTGGACTTTT TAGAGTCTGA CAGTTCCTGT
    TCCTCCGAAG CACTTTTGAA GAAAGAACTC TCTGCCGAAG AGCTGTATAA GCGACTCGAG
    AAACTCATTA TTGAGGACAA GGCAAATGAT GAACAGATCT TTGACTGGGT AGAGGCTAAT
    CTAGATGAAA GCCAGATGAG TTCACCTACA TTCCTTAGAG CTTTAATGAC AGCCGTCTGT
    AAAGCAGCTA TTATAGCCGA CTGTTCGACC TTCCGAGTGG ACACTGCTGT TATCAAGCAG
    AGAGTGCCGA TCTTACTCAA GTACCTAGAC TCAGATACAG AGAAGGAACT GCAAGCACTT
    TATGCACTAC AAGCATCAAT AGTAAAACTT GATCAACCTG CCAATTTGCT ACGGATGTTT
    TTTGATTGCC TGTATGACGA GGAGGTGATC TCTGAGGATG CCTTCTACAA GTGGGAGAGC
    AGCAAGGACC CGGCAGAGCA GAATGGGAAG GGTGTGGCCC TGAAGTCTGT CACGGCGTTC
    TTCACATGGC TGCGGGAAGC AGAAGAGGAG TCTGAGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03120
    Clone ID Related Accession (Same CDS sequence) XM_015460883.2 , XM_015462479.1 , XM_024982991.1 , XM_015460883.1
    Accession Version XM_015460883.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGTTTTTCC AGAGGCCTCA AATACAGCCT CCCAGAGCCA CCATCCCGAA CAGCAGCCCT
    TCCATTCGAC CCGGTGCACA GACACCCACT GCAGTGTATC AGGCCAATCA GCACATCATG
    ATGGTTAACC ATCTGCCCAT GCCGTACCCA GTGCCCCAGG GTCCTCAGTA CTGTATACCA
    CAGTACCGTC ACAGTGGCCC TCCGTATGTT GGGCCACCAC AACAGTACCC GGTTCAACCA
    CCAGGACCAG GTCCTTTTTA TCCTGGGCCA GGACCTGGGG ACTTCCCCAG TGCTTACGGA
    ACGCCTTTTT ACCCAAGTCA GCCAGTGTAC CAGTCAGCAC CTATCATAGT GCCTACGCAG
    CAGCAGCCCC CTCCAGCCAA GAGAGAGAAA AAAACAATAA GAATTCGGGA TCCAAACCAG
    GGAGGTAAAG ACATAACAGA GGAGATTATG TCTGGAGGTG GCAGCCGAAA TCCTACTCCA
    CCCATAGGGA GACCCACTTC CACACCTACT CCTCCTCAGC AGCTGCCCAG CCAGGTCCCC
    GAGCACAGCC CTGTGGTCTA TGGGACTGTG GAGAGCGCTC ATCTTGCTGC CAGCACCCCT
    GTCACTGCAG CGAGCGACCA GAAGCAAGAA GAGAAGCCAA AACCAGATCC AGTGTTAAAG
    ACTCCTTCCC CAGTCCTTAG GCTAGCCCTC AGTGGAGAGA AGAAAGAACA AGCAGGCCCG
    ACGTCTGAGA TTACTGCAGT AGAGTCCATA CCAGAGCTTC CTCTGCCTCC ATCCCCTACT
    GCTATTTCTC CACTTGCTCG AAGTACAATT ACTTCTCCCA CCTCTGTTGC TCTTAGTAGC
    CAACCAATAT TCACCACTGC AATTGATGAC AGATGTGAAC TCTCTTCCTC AAAAGAAGAT
    ACAATTCCTA TTCCCAGCCC CACATCTTGC ACAGAAGCAT CAGATCCTTT ACCAACAGAT
    GAAATTGATG ATGATATATG CAAGAAATCT TGTAGTGTAG CACCAAATGA TATTCCACTG
    ATTTCTAGTA CTAACCTAAT TAATGAAATG AATGGAGTTA ATGAAAAATT ACCAGCCACG
    GAAAGCATTG TGGAAATAGT AAAACAGGAG GTGTTACCGC TGACTCTTGA ATTGGAGATT
    TTGGAAAATC CCCCTGAAGA AATCAAAGTG GAGTGTGTCC CAACTCCCAT CACCCCTTCG
    ATAGTTCCCT CCTCTTCTCC GTCTCCTCCA GCTCCTCCAG CTTCTCCTTC TCCCACACCA
    GTTATCGTTC CTGCTGCTGC CACCAGTGTT AATACTGGTC GTGGTTCCAC CGGAGTCCAG
    AGAGCCTTCG AAGAGGGCGA GAACATAAGA ACTTGCCTTA GTGAAGATGC AAAAGAGATT
    CAGAACAAAG TAGAGGTAGA AGCAGATGGG CAAACAGAAG AGATTGTGGA TTCTCAGAAC
    TTAAGTTCAA GAAAGAGCCC AGTCCCAGCT CAGACAGCTA TACCTGCACC AAAGATGTGG
    AAGAAACCAA AAGACCGGAC CCGAGCCACT GAAGAGTCGT TAGAGGCAGA ATCAGAGCCT
    AAAGCTGAAG AGGAGCTTTC AGGTGACAAA ATACTTGAAT CTGAACAGGA TAAAATGAGC
    CAAGGGTTTC ATCCTGAAAG AGACCCCTCT GACCTAAAAA ACGTGAAAAC TGTGGAAGAA
    AATGGAGAAG AAGCTGAGCC TGTACGTAAT GGTGCTGAGA GTATTTCTGA GGGTGAAGGA
    ATAGATGCTA ATTCAGGCTC CACTGATAGT TCTGGTGAAG GGGTCACATT CCCATTTAAG
    TCAGAATCCT GGAAGCCTCC TGATTCGGAA GGCAAGAAGC AGTATGACAG GGAGTTTTTG
    CTGGATTTCC AATTCATGCC AGCGTGTATT CAGAAACCAG AGGGCCTGCC TCCCATCAGT
    GATGTGGTTC TTGACAAGAT CAATCAACCA AAATTGCCAA TGCGAACTCT GGATCCTCGG
    ATTTTGCCTC GAGGACCAGA CTTTACTCCA GCCTTTGCAG ATTTTGGAAG GCAAGCCCCT
    GGTGGAAGAG GTGTACCTGT TTGCAAAGTG CAGAGCAGGC ATGGATTGCC AATTCTGGAA
    CAGAGCAAAG CCCCAGCTTC CACTCCACTG GCGATGTCGC ACCCACCCCT GAAGAGCCTG
    CCTCTAGGGC TGTTGAATGT TGGAGCACGG AGATCTCAGC CTGGCCAGAG GAGAGAGCCC
    AGGAAGATCA TCACGGTCTC CGTGAAAGAA GACGTGCACC TGAAGAAGGC AGAGAACGCC
    TGGAAGCCCA GCCAGAGGCG GGATAGCCCA GCCGAGGACC CCGAGAACAT CAAGACCCAG
    GAACTTTTTA GAAAAGTTCG AAGTATCTTA AATAAATTGA CACCACAGAT GTTCAACCAA
    CTGATGAAGC AAGTGTCAGG ACTTACTGTT GACACAGAGG AGCGGCTGAA AGGAGTCATT
    GACCTGGTCT TTGAGAAGGC TATTGACGAA CCCAGTTTCT CTGTGGCTTA CGCAAACATG
    TGTCGATGTC TAGTAACGCT GAAAGTACCC ATGGCAGACA AGCCTGGTAA CACAGTGAAC
    TTCCGGAAGC TACTACTGAA CCGTTGCCAG AAGGAATTTG AAAAAGATAA AGCCGATGAT
    GATGTCTTTG AGAAGAAGCA GAAAGAACTC GAGGCTGCCA GTGCTCCAGA GGAGAGGACA
    AGGCTTCATG ATGAACTGGA AGAAGCTAAA GATAAAGCCC GGCGGAGATC CATTGGCAAC
    ATCAAGTTCA TTGGAGAACT CTTTAAACTC AAAATGCTGA CTGAAGCCAT CATGCATGAC
    TGTGTGGTGA AGCTGCTGAA GAACCACGAT GAAGAATCCC TCGAGTGTCT GTGTCGCCTG
    CTCACCACAA TTGGCAAAGA CCTGGACTTT GAGAAAGCAA AGCCACGTAT GGACCAGTAT
    TTTAATCAGA TGGAGAAAAT TGTAAAAGAA AGAAAAACCT CATCTAGGAT TCGATTCATG
    CTTCAGGACG TAATAGATCT AAGGCTGTGT AATTGGGTAT CACGACGAGC AGATCAGGGG
    CCTAAAACTA TTGAACAGAT TCACAAAGAA GCTAAAATAG AAGAACAGGA AGAGCAGAGG
    AAGGTCCAAC AACTCATGAC CAAAGAGAAG AGAAGACCAG GTGTCCAGAG AGTGGATGAA
    GGTGGGTGGA ACACTGTTCA AGGGGCCAAG AATAGTCGGG TATTGGACCC CTCAAAATTT
    CTGAAAATCA CTAAGCCCAC AATTGATGAG AAAATTCAGC TGGTACCTAA AGCGCAGCTG
    GGCAGCTGGG GAAAGGGCAG CAGTGGTGGA GCCAAGGCGA GCGAGGCCGA TGCCTTAAGA
    TCAAGTGCTT CCAGTTTAAA CAGATTCTCT GCTTTGCAAC CTCCAGCACC TTCAGGGTCT
    GCGTCATCCA CACCTCTAGA GTTTGATTCC CGGCGGACCT TAACTAGTCG AGGAAGCACG
    GGCAGGGAGA AGAATGACAA GCCCCTCCAG CCTGCAGCAG CTCGGCCGAA CACTTTCATG
    AGGGGTGGCA GCAGTAAAGA CCTGTTAGAC AATCAGTCTC AGGAGGAGCA GCGGAGGGAG
    ATGCTGGAGA CCGTGAAGCA GCTCACAGGA GGCATGGACG TGGAGAGGAG CAGCGCTGAG
    GCGGAGCGAA GCAGGACCAG GGAGCCAGTG ACAAAACCGG AAATTCCAGT AACATCAGCC
    TCTGACAAGC CCGCGTTGTC AGAGGAAGAG ATGGAGAGGA AGTCCAAATC TATCATTGAT
    GAGTTTCTAC ACATTAATGA TTTTAAGGAA GCCATGCAGT GTGTGGAAGA GCTGAACGCC
    CAGGGCCTGC AACATGTGTT CGTGAGGACG GGAGTGGAGT CCACCCTGGA AAGGAGCCAG
    ATCACCCGGG ATCACATGGG CCAGTTACTG TATCAGCTGG TGCAGTCAGA AAAGCTCAGC
    AAACAGGACT TTTTCAGAGG TTTTTCAGAA ACATTGGAAT TGGCAGATGA CATGGCCATT
    GATATTCCCC ATATTTGGTT GTACCTTGCT GAACTGGTGA CTCCCATGTT AAAAGAAGGC
    GGAATCTCCA TGAGACAACT CATCACAGAA TTTTGCAAAC CTTTACTTCC TGTTGGAAGA
    GCTGGAATCT TGATTTCTGA AATATTGCAC CTTCTATGCA AACAAATGAG CCATAAGAAA
    GTGGGAGCCC TATGGAGGGA GGCTGACCTC AGCTGGAAGG ACTTTTTACC AGAAGGGGAA
    GATGTACATA ATTTTCTCTT GGAGCAGAAG TTGGACTTTT TAGAGTCTGA CAGTTCCTGT
    TCCTCCGAAG CACTTTTGAA GAAAGAACTC TCTGCCGAAG AGCTGTATAA GCGACTCGAG
    AAACTCATTA TTGAGGACAA GGCAAATGAT GAACAGATCT TTGACTGGGT AGAGGCTAAT
    CTAGATGAAA GCCAGATGAG TTCACCTACA TTCCTTAGAG CTTTAATGAC AGCCGTCTGT
    AAAGCAGCTA TTATAGCCGA CTGTTCGACC TTCCGAGTGG ACACTGCTGT TATCAAGCAG
    AGAGTGCCGA TCTTACTCAA GTACCTAGAC TCAGATACAG AGAAGGAACT GCAAGCACTT
    TATGCACTAC AAGCATCAAT AGTAAAACTT GATCAACCTG CCAATTTGCT ACGGATGTTT
    TTTGATTGCC TGTATGACGA GGAGGTGATC TCTGAGGATG CCTTCTACAA GTGGGAGAGC
    AGCAAGGACC CGGCAGAGCA GAATGGGAAG GGTGTGGCCC TGAAGTCTGT CACGGCGTTC
    TTCACATGGC TGCGGGAAGC AGAAGAGGAG TCTGAGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316369.1
    CDS190..5094
    Translation

    Target ORF information:

    RefSeq Version XM_015460883.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460883.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGTTTTTCC AGAGGCCTCA AATACAGCCT CCCAGAGCCA CCATCCCGAA CAGCAGCCCT
    TCCATTCGAC CCGGTGCACA GACACCCACT GCAGTGTATC AGGCCAATCA GCACATCATG
    ATGGTTAACC ATCTGCCCAT GCCGTACCCA GTGCCCCAGG GTCCTCAGTA CTGTATACCA
    CAGTACCGTC ACAGTGGCCC TCCGTATGTT GGGCCACCAC AACAGTACCC GGTTCAACCA
    CCAGGACCAG GTCCTTTTTA TCCTGGGCCA GGACCTGGGG ACTTCCCCAG TGCTTACGGA
    ACGCCTTTTT ACCCAAGTCA GCCAGTGTAC CAGTCAGCAC CTATCATAGT GCCTACGCAG
    CAGCAGCCCC CTCCAGCCAA GAGAGAGAAA AAAACAATAA GAATTCGGGA TCCAAACCAG
    GGAGGTAAAG ACATAACAGA GGAGATTATG TCTGGAGGTG GCAGCCGAAA TCCTACTCCA
    CCCATAGGGA GACCCACTTC CACACCTACT CCTCCTCAGC AGCTGCCCAG CCAGGTCCCC
    GAGCACAGCC CTGTGGTCTA TGGGACTGTG GAGAGCGCTC ATCTTGCTGC CAGCACCCCT
    GTCACTGCAG CGAGCGACCA GAAGCAAGAA GAGAAGCCAA AACCAGATCC AGTGTTAAAG
    ACTCCTTCCC CAGTCCTTAG GCTAGCCCTC AGTGGAGAGA AGAAAGAACA AGCAGGCCCG
    ACGTCTGAGA TTACTGCAGT AGAGTCCATA CCAGAGCTTC CTCTGCCTCC ATCCCCTACT
    GCTATTTCTC CACTTGCTCG AAGTACAATT ACTTCTCCCA CCTCTGTTGC TCTTAGTAGC
    CAACCAATAT TCACCACTGC AATTGATGAC AGATGTGAAC TCTCTTCCTC AAAAGAAGAT
    ACAATTCCTA TTCCCAGCCC CACATCTTGC ACAGAAGCAT CAGATCCTTT ACCAACAGAT
    GAAATTGATG ATGATATATG CAAGAAATCT TGTAGTGTAG CACCAAATGA TATTCCACTG
    ATTTCTAGTA CTAACCTAAT TAATGAAATG AATGGAGTTA ATGAAAAATT ACCAGCCACG
    GAAAGCATTG TGGAAATAGT AAAACAGGAG GTGTTACCGC TGACTCTTGA ATTGGAGATT
    TTGGAAAATC CCCCTGAAGA AATCAAAGTG GAGTGTGTCC CAACTCCCAT CACCCCTTCG
    ATAGTTCCCT CCTCTTCTCC GTCTCCTCCA GCTCCTCCAG CTTCTCCTTC TCCCACACCA
    GTTATCGTTC CTGCTGCTGC CACCAGTGTT AATACTGGTC GTGGTTCCAC CGGAGTCCAG
    AGAGCCTTCG AAGAGGGCGA GAACATAAGA ACTTGCCTTA GTGAAGATGC AAAAGAGATT
    CAGAACAAAG TAGAGGTAGA AGCAGATGGG CAAACAGAAG AGATTGTGGA TTCTCAGAAC
    TTAAGTTCAA GAAAGAGCCC AGTCCCAGCT CAGACAGCTA TACCTGCACC AAAGATGTGG
    AAGAAACCAA AAGACCGGAC CCGAGCCACT GAAGAGTCGT TAGAGGCAGA ATCAGAGCCT
    AAAGCTGAAG AGGAGCTTTC AGGTGACAAA ATACTTGAAT CTGAACAGGA TAAAATGAGC
    CAAGGGTTTC ATCCTGAAAG AGACCCCTCT GACCTAAAAA ACGTGAAAAC TGTGGAAGAA
    AATGGAGAAG AAGCTGAGCC TGTACGTAAT GGTGCTGAGA GTATTTCTGA GGGTGAAGGA
    ATAGATGCTA ATTCAGGCTC CACTGATAGT TCTGGTGAAG GGGTCACATT CCCATTTAAG
    TCAGAATCCT GGAAGCCTCC TGATTCGGAA GGCAAGAAGC AGTATGACAG GGAGTTTTTG
    CTGGATTTCC AATTCATGCC AGCGTGTATT CAGAAACCAG AGGGCCTGCC TCCCATCAGT
    GATGTGGTTC TTGACAAGAT CAATCAACCA AAATTGCCAA TGCGAACTCT GGATCCTCGG
    ATTTTGCCTC GAGGACCAGA CTTTACTCCA GCCTTTGCAG ATTTTGGAAG GCAAGCCCCT
    GGTGGAAGAG GTGTACCTGT TTGCAAAGTG CAGAGCAGGC ATGGATTGCC AATTCTGGAA
    CAGAGCAAAG CCCCAGCTTC CACTCCACTG GCGATGTCGC ACCCACCCCT GAAGAGCCTG
    CCTCTAGGGC TGTTGAATGT TGGAGCACGG AGATCTCAGC CTGGCCAGAG GAGAGAGCCC
    AGGAAGATCA TCACGGTCTC CGTGAAAGAA GACGTGCACC TGAAGAAGGC AGAGAACGCC
    TGGAAGCCCA GCCAGAGGCG GGATAGCCCA GCCGAGGACC CCGAGAACAT CAAGACCCAG
    GAACTTTTTA GAAAAGTTCG AAGTATCTTA AATAAATTGA CACCACAGAT GTTCAACCAA
    CTGATGAAGC AAGTGTCAGG ACTTACTGTT GACACAGAGG AGCGGCTGAA AGGAGTCATT
    GACCTGGTCT TTGAGAAGGC TATTGACGAA CCCAGTTTCT CTGTGGCTTA CGCAAACATG
    TGTCGATGTC TAGTAACGCT GAAAGTACCC ATGGCAGACA AGCCTGGTAA CACAGTGAAC
    TTCCGGAAGC TACTACTGAA CCGTTGCCAG AAGGAATTTG AAAAAGATAA AGCCGATGAT
    GATGTCTTTG AGAAGAAGCA GAAAGAACTC GAGGCTGCCA GTGCTCCAGA GGAGAGGACA
    AGGCTTCATG ATGAACTGGA AGAAGCTAAA GATAAAGCCC GGCGGAGATC CATTGGCAAC
    ATCAAGTTCA TTGGAGAACT CTTTAAACTC AAAATGCTGA CTGAAGCCAT CATGCATGAC
    TGTGTGGTGA AGCTGCTGAA GAACCACGAT GAAGAATCCC TCGAGTGTCT GTGTCGCCTG
    CTCACCACAA TTGGCAAAGA CCTGGACTTT GAGAAAGCAA AGCCACGTAT GGACCAGTAT
    TTTAATCAGA TGGAGAAAAT TGTAAAAGAA AGAAAAACCT CATCTAGGAT TCGATTCATG
    CTTCAGGACG TAATAGATCT AAGGCTGTGT AATTGGGTAT CACGACGAGC AGATCAGGGG
    CCTAAAACTA TTGAACAGAT TCACAAAGAA GCTAAAATAG AAGAACAGGA AGAGCAGAGG
    AAGGTCCAAC AACTCATGAC CAAAGAGAAG AGAAGACCAG GTGTCCAGAG AGTGGATGAA
    GGTGGGTGGA ACACTGTTCA AGGGGCCAAG AATAGTCGGG TATTGGACCC CTCAAAATTT
    CTGAAAATCA CTAAGCCCAC AATTGATGAG AAAATTCAGC TGGTACCTAA AGCGCAGCTG
    GGCAGCTGGG GAAAGGGCAG CAGTGGTGGA GCCAAGGCGA GCGAGGCCGA TGCCTTAAGA
    TCAAGTGCTT CCAGTTTAAA CAGATTCTCT GCTTTGCAAC CTCCAGCACC TTCAGGGTCT
    GCGTCATCCA CACCTCTAGA GTTTGATTCC CGGCGGACCT TAACTAGTCG AGGAAGCACG
    GGCAGGGAGA AGAATGACAA GCCCCTCCAG CCTGCAGCAG CTCGGCCGAA CACTTTCATG
    AGGGGTGGCA GCAGTAAAGA CCTGTTAGAC AATCAGTCTC AGGAGGAGCA GCGGAGGGAG
    ATGCTGGAGA CCGTGAAGCA GCTCACAGGA GGCATGGACG TGGAGAGGAG CAGCGCTGAG
    GCGGAGCGAA GCAGGACCAG GGAGCCAGTG ACAAAACCGG AAATTCCAGT AACATCAGCC
    TCTGACAAGC CCGCGTTGTC AGAGGAAGAG ATGGAGAGGA AGTCCAAATC TATCATTGAT
    GAGTTTCTAC ACATTAATGA TTTTAAGGAA GCCATGCAGT GTGTGGAAGA GCTGAACGCC
    CAGGGCCTGC AACATGTGTT CGTGAGGACG GGAGTGGAGT CCACCCTGGA AAGGAGCCAG
    ATCACCCGGG ATCACATGGG CCAGTTACTG TATCAGCTGG TGCAGTCAGA AAAGCTCAGC
    AAACAGGACT TTTTCAGAGG TTTTTCAGAA ACATTGGAAT TGGCAGATGA CATGGCCATT
    GATATTCCCC ATATTTGGTT GTACCTTGCT GAACTGGTGA CTCCCATGTT AAAAGAAGGC
    GGAATCTCCA TGAGACAACT CATCACAGAA TTTTGCAAAC CTTTACTTCC TGTTGGAAGA
    GCTGGAATCT TGATTTCTGA AATATTGCAC CTTCTATGCA AACAAATGAG CCATAAGAAA
    GTGGGAGCCC TATGGAGGGA GGCTGACCTC AGCTGGAAGG ACTTTTTACC AGAAGGGGAA
    GATGTACATA ATTTTCTCTT GGAGCAGAAG TTGGACTTTT TAGAGTCTGA CAGTTCCTGT
    TCCTCCGAAG CACTTTTGAA GAAAGAACTC TCTGCCGAAG AGCTGTATAA GCGACTCGAG
    AAACTCATTA TTGAGGACAA GGCAAATGAT GAACAGATCT TTGACTGGGT AGAGGCTAAT
    CTAGATGAAA GCCAGATGAG TTCACCTACA TTCCTTAGAG CTTTAATGAC AGCCGTCTGT
    AAAGCAGCTA TTATAGCCGA CTGTTCGACC TTCCGAGTGG ACACTGCTGT TATCAAGCAG
    AGAGTGCCGA TCTTACTCAA GTACCTAGAC TCAGATACAG AGAAGGAACT GCAAGCACTT
    TATGCACTAC AAGCATCAAT AGTAAAACTT GATCAACCTG CCAATTTGCT ACGGATGTTT
    TTTGATTGCC TGTATGACGA GGAGGTGATC TCTGAGGATG CCTTCTACAA GTGGGAGAGC
    AGCAAGGACC CGGCAGAGCA GAATGGGAAG GGTGTGGCCC TGAAGTCTGT CACGGCGTTC
    TTCACATGGC TGCGGGAAGC AGAAGAGGAG TCTGAGGATA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03122
    Clone ID Related Accession (Same CDS sequence) XM_015460885.1 , XM_024983019.1 , XM_010802672.1 , XM_010826706.2 , XM_015462481.1 , XM_024982942.1 , XM_010802671.2 , XM_010826709.1 , XM_024982993.1
    Accession Version XM_015460885.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316371.1
    CDS450..5489
    Translation

    Target ORF information:

    RefSeq Version XM_015460885.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460885.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03122
    Clone ID Related Accession (Same CDS sequence) XM_015460885.1 , XM_024983019.1 , XM_010802672.1 , XM_010826706.2 , XM_015462481.1 , XM_024982942.1 , XM_010802671.2 , XM_010826709.1 , XM_024982993.1
    Accession Version XM_024983019.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838787.1
    CDS336..5375
    Translation

    Target ORF information:

    RefSeq Version XM_024983019.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983019.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03122
    Clone ID Related Accession (Same CDS sequence) XM_015460885.1 , XM_024983019.1 , XM_010802672.1 , XM_010826706.2 , XM_015462481.1 , XM_024982942.1 , XM_010802671.2 , XM_010826709.1 , XM_024982993.1
    Accession Version XM_010802672.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800974.1
    CDS289..5328
    Translation

    Target ORF information:

    RefSeq Version XM_010802672.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802672.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03122
    Clone ID Related Accession (Same CDS sequence) XM_015460885.1 , XM_024983019.1 , XM_010802672.1 , XM_010826706.2 , XM_015462481.1 , XM_024982942.1 , XM_010802671.2 , XM_010826709.1 , XM_024982993.1
    Accession Version XM_010826706.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826706.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010825008.1
    CDS579..5618
    Translation

    Target ORF information:

    RefSeq Version XM_010826706.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826706.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03122
    Clone ID Related Accession (Same CDS sequence) XM_015460885.1 , XM_024983019.1 , XM_010802672.1 , XM_010826706.2 , XM_015462481.1 , XM_024982942.1 , XM_010802671.2 , XM_010826709.1 , XM_024982993.1
    Accession Version XM_015462481.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317967.1
    CDS450..5489
    Translation

    Target ORF information:

    RefSeq Version XM_015462481.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462481.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03122
    Clone ID Related Accession (Same CDS sequence) XM_015460885.1 , XM_024983019.1 , XM_010802672.1 , XM_010826706.2 , XM_015462481.1 , XM_024982942.1 , XM_010802671.2 , XM_010826709.1 , XM_024982993.1
    Accession Version XM_024982942.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838710.1
    CDS75..5114
    Translation

    Target ORF information:

    RefSeq Version XM_024982942.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982942.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03122
    Clone ID Related Accession (Same CDS sequence) XM_015460885.1 , XM_024983019.1 , XM_010802672.1 , XM_010826706.2 , XM_015462481.1 , XM_024982942.1 , XM_010802671.2 , XM_010826709.1 , XM_024982993.1
    Accession Version XM_010802671.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802671.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800973.1
    CDS579..5618
    Translation

    Target ORF information:

    RefSeq Version XM_010802671.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802671.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03122
    Clone ID Related Accession (Same CDS sequence) XM_015460885.1 , XM_024983019.1 , XM_010802672.1 , XM_010826706.2 , XM_015462481.1 , XM_024982942.1 , XM_010802671.2 , XM_010826709.1 , XM_024982993.1
    Accession Version XM_010826709.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010825011.1
    CDS289..5328
    Translation

    Target ORF information:

    RefSeq Version XM_010826709.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826709.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03122
    Clone ID Related Accession (Same CDS sequence) XM_015460885.1 , XM_024983019.1 , XM_010802672.1 , XM_010826706.2 , XM_015462481.1 , XM_024982942.1 , XM_010802671.2 , XM_010826709.1 , XM_024982993.1
    Accession Version XM_024982993.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838761.1
    CDS465..5504
    Translation

    Target ORF information:

    RefSeq Version XM_024982993.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982993.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG CCCAGCAGAA CAGTGCCAAT ACATTGCACA 
    GACAACTGGA AGAGGAGGAA GGTTTTGGAA CAGACTCCCG TTTACAGGTC CTTGGCTGGA
    AGAGGCTGGA TAAAATACTG CATTTTTGCA GCGGGGCCTC GACCTGCCCA TCATCAGGGA
    GGATTCAGAC CCATCCAGTT TTTCCAGAGG CCTCAAATAC AGCCTCCCAG AGCCACCATC
    CCGAACAGCA GCCCTTCCAT TCGACCCGGT GCACAGACAC CCACTGCAGT GTATCAGGCC
    AATCAGCACA TCATGATGGT TAACCATCTG CCCATGCCGT ACCCAGTGCC CCAGGGTCCT
    CAGTACTGTA TACCACAGTA CCGTCACAGT GGCCCTCCGT ATGTTGGGCC ACCACAACAG
    TACCCGGTTC AACCACCAGG ACCAGGTCCT TTTTATCCTG GGCCAGGACC TGGGGACTTC
    CCCAGTGCTT ACGGAACGCC TTTTTACCCA AGTCAGCCAG TGTACCAGTC AGCACCTATC
    ATAGTGCCTA CGCAGCAGCA GCCCCCTCCA GCCAAGAGAG AGAAAAAAAC AATAAGAATT
    CGGGATCCAA ACCAGGGAGG TAAAGACATA ACAGAGGAGA TTATGTCTGG AGGTGGCAGC
    CGAAATCCTA CTCCACCCAT AGGGAGACCC ACTTCCACAC CTACTCCTCC TCAGCAGCTG
    CCCAGCCAGG TCCCCGAGCA CAGCCCTGTG GTCTATGGGA CTGTGGAGAG CGCTCATCTT
    GCTGCCAGCA CCCCTGTCAC TGCAGCGAGC GACCAGAAGC AAGAAGAGAA GCCAAAACCA
    GATCCAGTGT TAAAGACTCC TTCCCCAGTC CTTAGGCTAG CCCTCAGTGG AGAGAAGAAA
    GAACAAGCAG GCCCGACGTC TGAGATTACT GCAGTAGAGT CCATACCAGA GCTTCCTCTG
    CCTCCATCCC CTACTGCTAT TTCTCCACTT GCTCGAAGTA CAATTACTTC TCCCACCTCT
    GTTGCTCTTA GTAGCCAACC AATATTCACC ACTGCAATTG ATGACAGATG TGAACTCTCT
    TCCTCAAAAG AAGATACAAT TCCTATTCCC AGCCCCACAT CTTGCACAGA AGCATCAGAT
    CCTTTACCAA CAGATGAAAT TGATGATGAT ATATGCAAGA AATCTTGTAG TGTAGCACCA
    AATGATATTC CACTGATTTC TAGTACTAAC CTAATTAATG AAATGAATGG AGTTAATGAA
    AAATTACCAG CCACGGAAAG CATTGTGGAA ATAGTAAAAC AGGAGGTGTT ACCGCTGACT
    CTTGAATTGG AGATTTTGGA AAATCCCCCT GAAGAAATCA AAGTGGAGTG TGTCCCAACT
    CCCATCACCC CTTCGATAGT TCCCTCCTCT TCTCCGTCTC CTCCAGCTCC TCCAGCTTCT
    CCTTCTCCCA CACCAGTTAT CGTTCCTGCT GCTGCCACCA GTGTTAATAC TGGTCGTGGT
    TCCACCGGAG TCCAGAGAGC CTTCGAAGAG GGCGAGAACA TAAGAACTTG CCTTAGTGAA
    GATGCAAAAG AGATTCAGAA CAAAGTAGAG GTAGAAGCAG ATGGGCAAAC AGAAGAGATT
    GTGGATTCTC AGAACTTAAG TTCAAGAAAG AGCCCAGTCC CAGCTCAGAC AGCTATACCT
    GCACCAAAGA TGTGGAAGAA ACCAAAAGAC CGGACCCGAG CCACTGAAGA GTCGTTAGAG
    GCAGAATCAG AGCCTAAAGC TGAAGAGGAG CTTTCAGGTG ACAAAATACT TGAATCTGAA
    CAGGATAAAA TGAGCCAAGG GTTTCATCCT GAAAGAGACC CCTCTGACCT AAAAAACGTG
    AAAACTGTGG AAGAAAATGG AGAAGAAGCT GAGCCTGTAC GTAATGGTGC TGAGAGTATT
    TCTGAGGGTG AAGGAATAGA TGCTAATTCA GGCTCCACTG ATAGTTCTGG TGAAGGGGTC
    ACATTCCCAT TTAAGTCAGA ATCCTGGAAG CCTCCTGATT CGGAAGGCAA GAAGCAGTAT
    GACAGGGAGT TTTTGCTGGA TTTCCAATTC ATGCCAGCGT GTATTCAGAA ACCAGAGGGC
    CTGCCTCCCA TCAGTGATGT GGTTCTTGAC AAGATCAATC AACCAAAATT GCCAATGCGA
    ACTCTGGATC CTCGGATTTT GCCTCGAGGA CCAGACTTTA CTCCAGCCTT TGCAGATTTT
    GGAAGGCAAG CCCCTGGTGG AAGAGGTGTA CCTGTTTGCA AAGTGCAGAG CAGGCATGGA
    TTGCCAATTC TGGAACAGAG CAAAGCCCCA GCTTCCACTC CACTGGCGAT GTCGCACCCA
    CCCCTGAAGA GCCTGCCTCT AGGGCTGTTG AATGTTGGAG CACGGAGATC TCAGCCTGGC
    CAGAGGAGAG AGCCCAGGAA GATCATCACG GTCTCCGTGA AAGAAGACGT GCACCTGAAG
    AAGGCAGAGA ACGCCTGGAA GCCCAGCCAG AGGCGGGATA GCCCAGCCGA GGACCCCGAG
    AACATCAAGA CCCAGGAACT TTTTAGAAAA GTTCGAAGTA TCTTAAATAA ATTGACACCA
    CAGATGTTCA ACCAACTGAT GAAGCAAGTG TCAGGACTTA CTGTTGACAC AGAGGAGCGG
    CTGAAAGGAG TCATTGACCT GGTCTTTGAG AAGGCTATTG ACGAACCCAG TTTCTCTGTG
    GCTTACGCAA ACATGTGTCG ATGTCTAGTA ACGCTGAAAG TACCCATGGC AGACAAGCCT
    GGTAACACAG TGAACTTCCG GAAGCTACTA CTGAACCGTT GCCAGAAGGA ATTTGAAAAA
    GATAAAGCCG ATGATGATGT CTTTGAGAAG AAGCAGAAAG AACTCGAGGC TGCCAGTGCT
    CCAGAGGAGA GGACAAGGCT TCATGATGAA CTGGAAGAAG CTAAAGATAA AGCCCGGCGG
    AGATCCATTG GCAACATCAA GTTCATTGGA GAACTCTTTA AACTCAAAAT GCTGACTGAA
    GCCATCATGC ATGACTGTGT GGTGAAGCTG CTGAAGAACC ACGATGAAGA ATCCCTCGAG
    TGTCTGTGTC GCCTGCTCAC CACAATTGGC AAAGACCTGG ACTTTGAGAA AGCAAAGCCA
    CGTATGGACC AGTATTTTAA TCAGATGGAG AAAATTGTAA AAGAAAGAAA AACCTCATCT
    AGGATTCGAT TCATGCTTCA GGACGTAATA GATCTAAGGC TGTGTAATTG GGTATCACGA
    CGAGCAGATC AGGGGCCTAA AACTATTGAA CAGATTCACA AAGAAGCTAA AATAGAAGAA
    CAGGAAGAGC AGAGGAAGGT CCAACAACTC ATGACCAAAG AGAAGAGAAG ACCAGGTGTC
    CAGAGAGTGG ATGAAGGTGG GTGGAACACT GTTCAAGGGG CCAAGAATAG TCGGGTATTG
    GACCCCTCAA AATTTCTGAA AATCACTAAG CCCACAATTG ATGAGAAAAT TCAGCTGGTA
    CCTAAAGCGC AGCTGGGCAG CTGGGGAAAG GGCAGCAGTG GTGGAGCCAA GGCGAGCGAG
    GCCGATGCCT TAAGATCAAG TGCTTCCAGT TTAAACAGAT TCTCTGCTTT GCAACCTCCA
    GCACCTTCAG GGTCTGCGTC ATCCACACCT CTAGAGTTTG ATTCCCGGCG GACCTTAACT
    AGTCGAGGAA GCACGGGCAG GGAGAAGAAT GACAAGCCCC TCCAGCCTGC AGCAGCTCGG
    CCGAACACTT TCATGAGGGG TGGCAGCAGT AAAGACCTGT TAGACAATCA GTCTCAGGAG
    GAGCAGCGGA GGGAGATGCT GGAGACCGTG AAGCAGCTCA CAGGAGGCAT GGACGTGGAG
    AGGAGCAGCG CTGAGGCGGA GCGAAGCAGG ACCAGGGAGC CAGTGACAAA ACCGGAAATT
    CCAGTAACAT CAGCCTCTGA CAAGCCCGCG TTGTCAGAGG AAGAGATGGA GAGGAAGTCC
    AAATCTATCA TTGATGAGTT TCTACACATT AATGATTTTA AGGAAGCCAT GCAGTGTGTG
    GAAGAGCTGA ACGCCCAGGG CCTGCAACAT GTGTTCGTGA GGACGGGAGT GGAGTCCACC
    CTGGAAAGGA GCCAGATCAC CCGGGATCAC ATGGGCCAGT TACTGTATCA GCTGGTGCAG
    TCAGAAAAGC TCAGCAAACA GGACTTTTTC AGAGGTTTTT CAGAAACATT GGAATTGGCA
    GATGACATGG CCATTGATAT TCCCCATATT TGGTTGTACC TTGCTGAACT GGTGACTCCC
    ATGTTAAAAG AAGGCGGAAT CTCCATGAGA CAACTCATCA CAGAATTTTG CAAACCTTTA
    CTTCCTGTTG GAAGAGCTGG AATCTTGATT TCTGAAATAT TGCACCTTCT ATGCAAACAA
    ATGAGCCATA AGAAAGTGGG AGCCCTATGG AGGGAGGCTG ACCTCAGCTG GAAGGACTTT
    TTACCAGAAG GGGAAGATGT ACATAATTTT CTCTTGGAGC AGAAGTTGGA CTTTTTAGAG
    TCTGACAGTT CCTGTTCCTC CGAAGCACTT TTGAAGAAAG AACTCTCTGC CGAAGAGCTG
    TATAAGCGAC TCGAGAAACT CATTATTGAG GACAAGGCAA ATGATGAACA GATCTTTGAC
    TGGGTAGAGG CTAATCTAGA TGAAAGCCAG ATGAGTTCAC CTACATTCCT TAGAGCTTTA
    ATGACAGCCG TCTGTAAAGC AGCTATTATA GCCGACTGTT CGACCTTCCG AGTGGACACT
    GCTGTTATCA AGCAGAGAGT GCCGATCTTA CTCAAGTACC TAGACTCAGA TACAGAGAAG
    GAACTGCAAG CACTTTATGC ACTACAAGCA TCAATAGTAA AACTTGATCA ACCTGCCAAT
    TTGCTACGGA TGTTTTTTGA TTGCCTGTAT GACGAGGAGG TGATCTCTGA GGATGCCTTC
    TACAAGTGGG AGAGCAGCAA GGACCCGGCA GAGCAGAATG GGAAGGGTGT GGCCCTGAAG
    TCTGTCACGG CGTTCTTCAC ATGGCTGCGG GAAGCAGAAG AGGAGTCTGA GGATAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52861
    Clone ID Related Accession (Same CDS sequence) XM_015460880.1 , XM_015462478.1 , XM_015460880.2 , XM_024982968.1 , XM_024982977.1
    Accession Version XM_015460880.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGGGAGGAT TCAGACCCAT CCAGTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316366.1
    CDS162..5087
    Translation

    Target ORF information:

    RefSeq Version XM_015460880.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460880.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGGGAGGAT TCAGACCCAT CCAGTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52861
    Clone ID Related Accession (Same CDS sequence) XM_015460880.1 , XM_015462478.1 , XM_015460880.2 , XM_024982968.1 , XM_024982977.1
    Accession Version XM_015462478.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGGGAGGAT TCAGACCCAT CCAGTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317964.1
    CDS162..5087
    Translation

    Target ORF information:

    RefSeq Version XM_015462478.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462478.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGGGAGGAT TCAGACCCAT CCAGTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52861
    Clone ID Related Accession (Same CDS sequence) XM_015460880.1 , XM_015462478.1 , XM_015460880.2 , XM_024982968.1 , XM_024982977.1
    Accession Version XM_015460880.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGGGAGGAT TCAGACCCAT CCAGTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316366.1
    CDS196..5121
    Translation

    Target ORF information:

    RefSeq Version XM_015460880.2
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460880.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGGGAGGAT TCAGACCCAT CCAGTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52861
    Clone ID Related Accession (Same CDS sequence) XM_015460880.1 , XM_015462478.1 , XM_015460880.2 , XM_024982968.1 , XM_024982977.1
    Accession Version XM_024982968.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGGGAGGAT TCAGACCCAT CCAGTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838736.1
    CDS494..5419
    Translation

    Target ORF information:

    RefSeq Version XM_024982968.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982968.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGGGAGGAT TCAGACCCAT CCAGTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52861
    Clone ID Related Accession (Same CDS sequence) XM_015460880.1 , XM_015462478.1 , XM_015460880.2 , XM_024982968.1 , XM_024982977.1
    Accession Version XM_024982977.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product eukaryotic translation initiation factor 4 gamma 3 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGGGAGGAT TCAGACCCAT CCAGTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838745.1
    CDS75..5000
    Translation

    Target ORF information:

    RefSeq Version XM_024982977.1
    Organism Bos taurus(cattle)
    Definition Bos taurus eukaryotic translation initiation factor 4 gamma 3 (EIF4G3), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982977.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATTCAC AACCTCAAAC CCGTTCTCCG TTTGCAGCGG GGCCTCGACC TGCCCATCAT 
    CAGGGAGGAT TCAGACCCAT CCAGTTTTTC CAGAGGCCTC AAATACAGCC TCCCAGAGCC
    ACCATCCCGA ACAGCAGCCC TTCCATTCGA CCCGGTGCAC AGACACCCAC TGCAGTGTAT
    CAGGCCAATC AGCACATCAT GATGGTTAAC CATCTGCCCA TGCCGTACCC AGTGCCCCAG
    GGTCCTCAGT ACTGTATACC ACAGTACCGT CACAGTGGCC CTCCGTATGT TGGGCCACCA
    CAACAGTACC CGGTTCAACC ACCAGGACCA GGTCCTTTTT ATCCTGGGCC AGGACCTGGG
    GACTTCCCCA GTGCTTACGG AACGCCTTTT TACCCAAGTC AGCCAGTGTA CCAGTCAGCA
    CCTATCATAG TGCCTACGCA GCAGCAGCCC CCTCCAGCCA AGAGAGAGAA AAAAACAATA
    AGAATTCGGG ATCCAAACCA GGGAGGTAAA GACATAACAG AGGAGATTAT GTCTGGAGGT
    GGCAGCCGAA ATCCTACTCC ACCCATAGGG AGACCCACTT CCACACCTAC TCCTCCTCAG
    CAGCTGCCCA GCCAGGTCCC CGAGCACAGC CCTGTGGTCT ATGGGACTGT GGAGAGCGCT
    CATCTTGCTG CCAGCACCCC TGTCACTGCA GCGAGCGACC AGAAGCAAGA AGAGAAGCCA
    AAACCAGATC CAGTGTTAAA GACTCCTTCC CCAGTCCTTA GGCTAGCCCT CAGTGGAGAG
    AAGAAAGAAC AAGCAGGCCC GACGTCTGAG ATTACTGCAG TAGAGTCCAT ACCAGAGCTT
    CCTCTGCCTC CATCCCCTAC TGCTATTTCT CCACTTGCTC GAAGTACAAT TACTTCTCCC
    ACCTCTGTTG CTCTTAGTAG CCAACCAATA TTCACCACTG CAATTGATGA CAGATGTGAA
    CTCTCTTCCT CAAAAGAAGA TACAATTCCT ATTCCCAGCC CCACATCTTG CACAGAAGCA
    TCAGATCCTT TACCAACAGA TGAAATTGAT GATGATATAT GCAAGAAATC TTGTAGTGTA
    GCACCAAATG ATATTCCACT GATTTCTAGT ACTAACCTAA TTAATGAAAT GAATGGAGTT
    AATGAAAAAT TACCAGCCAC GGAAAGCATT GTGGAAATAG TAAAACAGGA GGTGTTACCG
    CTGACTCTTG AATTGGAGAT TTTGGAAAAT CCCCCTGAAG AAATCAAAGT GGAGTGTGTC
    CCAACTCCCA TCACCCCTTC GATAGTTCCC TCCTCTTCTC CGTCTCCTCC AGCTCCTCCA
    GCTTCTCCTT CTCCCACACC AGTTATCGTT CCTGCTGCTG CCACCAGTGT TAATACTGGT
    CGTGGTTCCA CCGGAGTCCA GAGAGCCTTC GAAGAGGGCG AGAACATAAG AACTTGCCTT
    AGTGAAGATG CAAAAGAGAT TCAGAACAAA GTAGAGGTAG AAGCAGATGG GCAAACAGAA
    GAGATTGTGG ATTCTCAGAA CTTAAGTTCA AGAAAGAGCC CAGTCCCAGC TCAGACAGCT
    ATACCTGCAC CAAAGATGTG GAAGAAACCA AAAGACCGGA CCCGAGCCAC TGAAGAGTCG
    TTAGAGGCAG AATCAGAGCC TAAAGCTGAA GAGGAGCTTT CAGGTGACAA AATACTTGAA
    TCTGAACAGG ATAAAATGAG CCAAGGGTTT CATCCTGAAA GAGACCCCTC TGACCTAAAA
    AACGTGAAAA CTGTGGAAGA AAATGGAGAA GAAGCTGAGC CTGTACGTAA TGGTGCTGAG
    AGTATTTCTG AGGGTGAAGG AATAGATGCT AATTCAGGCT CCACTGATAG TTCTGGTGAA
    GGGGTCACAT TCCCATTTAA GTCAGAATCC TGGAAGCCTC CTGATTCGGA AGGCAAGAAG
    CAGTATGACA GGGAGTTTTT GCTGGATTTC CAATTCATGC CAGCGTGTAT TCAGAAACCA
    GAGGGCCTGC CTCCCATCAG TGATGTGGTT CTTGACAAGA TCAATCAACC AAAATTGCCA
    ATGCGAACTC TGGATCCTCG GATTTTGCCT CGAGGACCAG ACTTTACTCC AGCCTTTGCA
    GATTTTGGAA GGCAAGCCCC TGGTGGAAGA GGTGTACCTG TTTGCAAAGT GCAGAGCAGG
    CATGGATTGC CAATTCTGGA ACAGAGCAAA GCCCCAGCTT CCACTCCACT GGCGATGTCG
    CACCCACCCC TGAAGAGCCT GCCTCTAGGG CTGTTGAATG TTGGAGCACG GAGATCTCAG
    CCTGGCCAGA GGAGAGAGCC CAGGAAGATC ATCACGGTCT CCGTGAAAGA AGACGTGCAC
    CTGAAGAAGG CAGAGAACGC CTGGAAGCCC AGCCAGAGGC GGGATAGCCC AGCCGAGGAC
    CCCGAGAACA TCAAGACCCA GGAACTTTTT AGAAAAGTTC GAAGTATCTT AAATAAATTG
    ACACCACAGA TGTTCAACCA ACTGATGAAG CAAGTGTCAG GACTTACTGT TGACACAGAG
    GAGCGGCTGA AAGGAGTCAT TGACCTGGTC TTTGAGAAGG CTATTGACGA ACCCAGTTTC
    TCTGTGGCTT ACGCAAACAT GTGTCGATGT CTAGTAACGC TGAAAGTACC CATGGCAGAC
    AAGCCTGGTA ACACAGTGAA CTTCCGGAAG CTACTACTGA ACCGTTGCCA GAAGGAATTT
    GAAAAAGATA AAGCCGATGA TGATGTCTTT GAGAAGAAGC AGAAAGAACT CGAGGCTGCC
    AGTGCTCCAG AGGAGAGGAC AAGGCTTCAT GATGAACTGG AAGAAGCTAA AGATAAAGCC
    CGGCGGAGAT CCATTGGCAA CATCAAGTTC ATTGGAGAAC TCTTTAAACT CAAAATGCTG
    ACTGAAGCCA TCATGCATGA CTGTGTGGTG AAGCTGCTGA AGAACCACGA TGAAGAATCC
    CTCGAGTGTC TGTGTCGCCT GCTCACCACA ATTGGCAAAG ACCTGGACTT TGAGAAAGCA
    AAGCCACGTA TGGACCAGTA TTTTAATCAG ATGGAGAAAA TTGTAAAAGA AAGAAAAACC
    TCATCTAGGA TTCGATTCAT GCTTCAGGAC GTAATAGATC TAAGGCTGTG TAATTGGGTA
    TCACGACGAG CAGATCAGGG GCCTAAAACT ATTGAACAGA TTCACAAAGA AGCTAAAATA
    GAAGAACAGG AAGAGCAGAG GAAGGTCCAA CAACTCATGA CCAAAGAGAA GAGAAGACCA
    GGTGTCCAGA GAGTGGATGA AGGTGGGTGG AACACTGTTC AAGGGGCCAA GAATAGTCGG
    GTATTGGACC CCTCAAAATT TCTGAAAATC ACTAAGCCCA CAATTGATGA GAAAATTCAG
    CTGGTACCTA AAGCGCAGCT GGGCAGCTGG GGAAAGGGCA GCAGTGGTGG AGCCAAGGCG
    AGCGAGGCCG ATGCCTTAAG ATCAAGTGCT TCCAGTTTAA ACAGATTCTC TGCTTTGCAA
    CCTCCAGCAC CTTCAGGGTC TGCGTCATCC ACACCTCTAG AGTTTGATTC CCGGCGGACC
    TTAACTAGTC GAGGAAGCAC GGGCAGGGAG AAGAATGACA AGCCCCTCCA GCCTGCAGCA
    GCTCGGCCGA ACACTTTCAT GAGGGGTGGC AGCAGTAAAG ACCTGTTAGA CAATCAGTCT
    CAGGAGGAGC AGCGGAGGGA GATGCTGGAG ACCGTGAAGC AGCTCACAGG AGGCATGGAC
    GTGGAGAGGA GCAGCGCTGA GGCGGAGCGA AGCAGGACCA GGGAGCCAGT GACAAAACCG
    GAAATTCCAG TAACATCAGC CTCTGACAAG CCCGCGTTGT CAGAGGAAGA GATGGAGAGG
    AAGTCCAAAT CTATCATTGA TGAGTTTCTA CACATTAATG ATTTTAAGGA AGCCATGCAG
    TGTGTGGAAG AGCTGAACGC CCAGGGCCTG CAACATGTGT TCGTGAGGAC GGGAGTGGAG
    TCCACCCTGG AAAGGAGCCA GATCACCCGG GATCACATGG GCCAGTTACT GTATCAGCTG
    GTGCAGTCAG AAAAGCTCAG CAAACAGGAC TTTTTCAGAG GTTTTTCAGA AACATTGGAA
    TTGGCAGATG ACATGGCCAT TGATATTCCC CATATTTGGT TGTACCTTGC TGAACTGGTG
    ACTCCCATGT TAAAAGAAGG CGGAATCTCC ATGAGACAAC TCATCACAGA ATTTTGCAAA
    CCTTTACTTC CTGTTGGAAG AGCTGGAATC TTGATTTCTG AAATATTGCA CCTTCTATGC
    AAACAAATGA GCCATAAGAA AGTGGGAGCC CTATGGAGGG AGGCTGACCT CAGCTGGAAG
    GACTTTTTAC CAGAAGGGGA AGATGTACAT AATTTTCTCT TGGAGCAGAA GTTGGACTTT
    TTAGAGTCTG ACAGTTCCTG TTCCTCCGAA GCACTTTTGA AGAAAGAACT CTCTGCCGAA
    GAGCTGTATA AGCGACTCGA GAAACTCATT ATTGAGGACA AGGCAAATGA TGAACAGATC
    TTTGACTGGG TAGAGGCTAA TCTAGATGAA AGCCAGATGA GTTCACCTAC ATTCCTTAGA
    GCTTTAATGA CAGCCGTCTG TAAAGCAGCT ATTATAGCCG ACTGTTCGAC CTTCCGAGTG
    GACACTGCTG TTATCAAGCA GAGAGTGCCG ATCTTACTCA AGTACCTAGA CTCAGATACA
    GAGAAGGAAC TGCAAGCACT TTATGCACTA CAAGCATCAA TAGTAAAACT TGATCAACCT
    GCCAATTTGC TACGGATGTT TTTTGATTGC CTGTATGACG AGGAGGTGAT CTCTGAGGAT
    GCCTTCTACA AGTGGGAGAG CAGCAAGGAC CCGGCAGAGC AGAATGGGAA GGGTGTGGCC
    CTGAAGTCTG TCACGGCGTT CTTCACATGG CTGCGGGAAG CAGAAGAGGA GTCTGAGGAT
    AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.